LOCUS       DATDNW010000001         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_273562, whole genome shotgun sequence.
ACCESSION   DATDNW010000001 DATDNW010000000
VERSION     DATDNW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273562"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VI359_00005"
     CDS             <1..266
                     /locus_tag="VI359_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY6972660.1"
                     /translation="HMPERSDTLVRDRLARQRTELANERTLLSYVRTSLGFFIVGIPA
                     VWWLELPGIQALGVVSLVTGVVFLAVGVWRFFTIKSMINQQPD"
     gene            403..2043
                     /locus_tag="VI359_00010"
     CDS             403..2043
                     /locus_tag="VI359_00010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY6972661.1"
                     /translation="MKAAKLLVRCLENEGVQLVFGLPGEETLELMDALLDSNIRFIET
                     RHEQGAAFMADVYGRLSGKAGVCLSTLGPGATNLLTGVVDAYLDRAPLVAITGQASLN
                     RRHKESHQYIDVISMFKPVTKWNASLPKAEVIPEAIRKAFKIAQTEKPGATHVELPED
                     VAEEALDDANTLEPLFVQAPVTPDPAPSQVARAVRAIAGAERPVVLAGNGVIRGHAHE
                     AVRSFARQLQIPVLHTFMAKGVYPDSDPLSLYTIGLQARDYATVVMEQADVVVAVGYD
                     FVEFAPCFWNPRRDKRIVHVDVSPAEVDEHYIVEVGVLGNIRLSLEDIAEDLAPFDPT
                     WARGARKTVVDGFEAELVGPGSWPMRPQHIMRDLRAVLEPDDLVVCDVGAHKLWMSRM
                     FPCEVPNSCIISNGFAAMGMSLPGAIAAKLLYPERRVVAVTGDGGFLMNSQELETAVR
                     LHLPLVILVWRDDGYGVIRWKQMVRFGRTSSVDFGNPEFVAYASSFGAVGYRVTDQSE
                     LRPILAEALKSKVPAVIDCPVDYGENLRLTDRLNALAH"
     gene            complement(2177..2401)
                     /locus_tag="VI359_00015"
     CDS             complement(2177..2401)
                     /locus_tag="VI359_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972662.1"
                     /translation="MKLVFSLLIALAVWLPTFAFAEGAGGTYRGIAQIYYTLIWVILS
                     YGLYDVFGKKAMYVGAPLLAIGIYLLLPPG"
     gene            complement(2704..>2914)
                     /locus_tag="VI359_00020"
     CDS             complement(2704..>2914)
                     /locus_tag="VI359_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972663.1"
                     /translation="GFVSNEGSLSASRTAADHYVIVTYWRSFEDHERSHANEIFNKKF
                     AVLAAMCTDTKELGYDMLWQGAPAS"
BASE COUNT          587 a          814 c          896 g          617 t
ORIGIN      
        1 ctcacatgcc ggaacgatcg gacacgctgg ttcgagaccg gctggctcgg caacgaaccg
       61 agctggcgaa cgagcgtacg ctgctgtcgt atgtcagaac ttcgctgggg tttttcatcg
      121 ttgggattcc cgccgtgtgg tggctggaat tgcctgggat tcaagccctg ggtgtggtgt
      181 cgctggtgac gggcgtcgtg ttcttggcgg tcggagtttg gcgattcttc accatcaaat
      241 cgatgatcaa ccaacagccg gactagccgc ccatacgctc acattcatca gtgagtaccg
      301 ggagatagaa tagaagatgc gcccctgcga ccgactcggc tgagcagcgg gtcacctctg
      361 ccgcgaaccg gttcgtgctc tcagatcgaa gaaaggcatt tcatgaaggc ggcaaagcta
      421 ctcgttcgtt gcctggaaaa tgaaggggtc caactggtct ttggtttgcc gggcgaggaa
      481 accctggagt tgatggatgc cctgctggat tccaacattc gctttatcga gacgcggcat
      541 gaacaaggag cggcgttcat ggccgacgtc tacgggcgtc tcagcgggaa agccggggtc
      601 tgcctgtcta cgctggggcc tggcgccacg aatctcctga cgggcgtcgt ggatgcctac
      661 ctggatcggg ccccgctggt cgcgattacc ggacaagcgt cgctgaatcg ccgccacaag
      721 gaatcgcacc agtacatcga tgtcatctcg atgttcaagc ccgtcaccaa atggaatgcc
      781 tcgctcccca aggcggaggt cattcccgaa gcaattcgga aggcgttcaa gatcgcccag
      841 acggaaaaac ctggcgcaac tcatgtagag ttgccggaag acgtggcgga agaggcgctt
      901 gacgacgcga acacgttgga gccgctcttt gtacaggccc ctgtcacacc tgacccggca
      961 ccgagccagg tcgcgagggc agtccgggcg atcgccggag cggagcgacc ggtagtcctc
     1021 gccgggaatg gcgtgatccg tgggcatgca cacgaggcgg tcagatcgtt cgcgcggcag
     1081 cttcagattc ccgtcctgca tacgtttatg gccaagggcg tttacccaga cagcgatccg
     1141 ctgtcgcttt atacgatcgg cctccaggcc agagattacg cgacggtcgt catggagcag
     1201 gccgacgtgg tcgttgccgt tgggtatgat tttgtggagt tcgccccctg cttctggaat
     1261 cccagacggg ataagcgcat tgtccacgtc gacgtgtcac cggcagaggt ggacgagcat
     1321 tacatcgtcg aagtgggcgt actggggaac attcgtcttt cgttggagga catcgccgag
     1381 gatctcgccc cgttcgatcc gacatgggcg cgaggtgctc gcaagactgt cgtcgatggg
     1441 ttcgaagccg aactggtcgg ccctggttca tggccgatgc ggcctcaaca catcatgcgg
     1501 gatctccgtg cggtcctcga gcccgacgat cttgtggtct gtgatgttgg cgcccacaaa
     1561 ctttggatgt cccggatgtt tccctgtgag gttcccaact cctgcattat ttcgaacggc
     1621 tttgctgcaa tgggaatgag tctgcccggt gcaattgccg ccaagttact gtatccggaa
     1681 cggcgcgtgg tcgcagtgac gggagacggc gggtttctca tgaactcgca ggagttggag
     1741 acggccgtgc ggttgcacct gccgttagtc attttggtat ggcgggacga cgggtacggc
     1801 gtgattcgtt ggaaacagat ggtgcggttt ggccggacct cttccgtaga tttcgggaat
     1861 ccggagttcg tcgcctacgc gagcagtttc ggtgccgtcg gctatcgggt gacagaccag
     1921 tcggagcttc gaccaatcct cgcggaagcg ctcaagagca aggtgccggc ggtcatcgat
     1981 tgtccggtag attatggaga gaatctccgg ctcacggatc gcttaaacgc ccttgcgcat
     2041 tgaatcaaat cttttcccca caccagtgcg tcgttgtaca catgcgcgca cgcatcgtca
     2101 aatcgggcgg tggcgatcga ccggaggccc tgagaacaca gagcagcacc tcccagggcc
     2161 tatcagttta tccgcgtcag cccgggggga ggagaagata gatgccgatt gccaggagcg
     2221 gagctcccac atacatggct tttttcccga acacatcgta caagccataa gataggatta
     2281 cccatatcaa cgtgtaataa atctgggcga tgccacgata ggtgcctccg gctccctccg
     2341 caaaggcgaa agtaggcagc cagacagcga gtgcgatcag gagactgaac accagtttca
     2401 tcacgctcct cctttgaaca ggtgggaatg cctcttgccg cgggggaaga ctgacgccac
     2461 gtgcaaacgc cctcacggca ggaccttacc gtgagggcgt cacattgttc cggatcgact
     2521 caaccgtatg gtatcgaaca acgctacccc ggctccattc gtggcctcgc tctgggggat
     2581 tcgctaatcc tgcgctttct caatcagcct gtcaacatga gggaacgcgt cgccaaggcg
     2641 tggaggttct attattccgg caagtcgccc gcgataggcg agactactgg ctcgctcgag
     2701 cgactaggat gccggcgcgc cctgccacag catgtcgtat cccagttcct tggtgtctgt
     2761 gcacatggct gctaaaacgg cgaacttctt attgaagatt tcattggcgt gggatcgttc
     2821 gtgatcttcg aacgagcgcc aataggtgac gatgacataa tggtcggcag cggtcctgga
     2881 tgccgagagg gagccttcat tcgaaacgaa cccg
//
LOCUS       DATDNW010000002         4108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1298010, whole genome shotgun sequence.
ACCESSION   DATDNW010000002 DATDNW010000000
VERSION     DATDNW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298010"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..429)
                     /gene="smbP"
                     /locus_tag="VI359_00025"
     CDS             complement(82..429)
                     /gene="smbP"
                     /locus_tag="VI359_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HEY6972664.1"
                     /translation="MKIQQGALIVAAVAALVGVPLFGGMALADNKHVSEAIEHAKEAV
                     SHGKQGHADALVQHAEGALKHAQAAQKDMKNPHLDEGVKHLQEAVEHGKAGHADVATK
                     HAEGAVTHLSEVK"
     gene            complement(535..1416)
                     /locus_tag="VI359_00030"
     CDS             complement(535..1416)
                     /locus_tag="VI359_00030"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY6972665.1"
                     /translation="MTHPLLIDTETLQKQLGQPGLVVIDVRGRAAYEFGGHIPGAINS
                     TWHEYSDPKAVAKGLLDPDIGRMQQKIRALGINDDSDIVIYSNPFDNWGDEGRMFWML
                     EYLGHQHLRILDGGWVKWVEEKRPFEHGPVRPKPGNFTVRLKTEVMANKDEVKQLVKQ
                     PPPGTVIIDARSLEEYLGKEVSGIPRPGHIPSAMHVGWNGFLQPNATVKDIEAIKASL
                     DEKGLHPDQAVVCYCTGGVRSAWLYFVLKLAGYRKVRNYPGSWWEWSRDFACPVEKDF
                     KGLQKVLGLVDEAARPS"
     gene            complement(1450..2739)
                     /locus_tag="VI359_00035"
     CDS             complement(1450..2739)
                     /locus_tag="VI359_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY6972666.1"
                     /translation="MSRGLKIVLGVVLAAGAGYVYYTEIKPVVIFGLRSDYAHAIPYQ
                     KVPAGLDSLKAESCGTCHREIYDEWKASIHAHAFADPFFQAYWKKDKNIWVCLNCHTP
                     LENQQPTLIKEIPRGRVEKAIQEPNPHYDAEYQKEAITCAACHVRDGIIYGPFEDSAA
                     PHPTKFDPNFRTTQICYRCHNVVSGPAQFYNAGPCGTYAEYEGKFFMRERGFICQSCH
                     MPEIDRPVATNGPIRRGRQHLWRGGHDPDMIKRAVAVQIKADPSHPKSGDQVTFTLTL
                     INAGAGHKIPTGDPDRHFTIEFLVEDRAKQVVHQDKSTMGRWIMWQPAIVELYDNRLL
                     PLASREYRFTYRMPAESEGLALKARVQYHILTDGQHEMLRTKYGLTANDPYRFEIYVR
                     EFPLTGNLAAALSRESKQQAAVSVPPESLSCKVRAES"
     gene            complement(2736..3680)
                     /locus_tag="VI359_00040"
     CDS             complement(2736..3680)
                     /locus_tag="VI359_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY6972667.1"
                     /translation="MGKTGLVYDPHYLEHDMGQGHPESPHRLRAIMQQLEQSGTLRQL
                     TRIEPRRAEDEWVTQIHQPGYVAALAKHAPIQGRISLDPDTSMSPGSLTAAYLAAGGA
                     LAAVDAIMNHQVDHVFCAVRPPGHHAEAGRAMGFCLFNNIAIAARYAQKRYGLPRILI
                     VDWDVHHGNGTQHSFEDDSSILFFSTHQYPHYPGTGRGTECGTGGGEGFTINVPMEAG
                     GGDEDYRTVFRKTLVPAADTFKPDFVMISAGFDAHKDDPLASMELTDAGYADLTAIVA
                     GIAKRYAGGRILSTLEGGYNLTALAASVDSHLTALLNV"
     gene            3785..4102
                     /locus_tag="VI359_00045"
     CDS             3785..4102
                     /locus_tag="VI359_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972668.1"
                     /translation="MPNPRIEPLKKVLTIDPNDDVAWFGLGKAYMEDGNFDEAAKALQ
                     QCITVKPSYSAAYFALAQSLHKLGRIDECRAVCTSGIDVSTRNGDAMVTKNLEALTSS
                     LPT"
BASE COUNT          856 a         1220 c         1107 g          925 t
ORIGIN      
        1 actcgcacgc tgcgacctct ccagacaaca acgggcaggg gggactcccc tgcccgttgc
       61 atcagcttct gatctgatca actacttcac ttcggacaga tgcgtgacag ctccctcggc
      121 atgtttggtc gccacatccg catgaccggc cttgccgtgc tcgacggctt cctgcagatg
      181 cttgacgcct tcgtccaaat gcggattctt catatctttc tgggccgcct gtgcatgctt
      241 gagcgcccct tccgcatgtt gtacaagggc atcggcatgg ccctgctttc catgagacac
      301 ggcttccttc gcatgctcga tcgcttcgct tacatgctta ttgtcggcca gggccatccc
      361 cccaaatagc ggtacgccga ccagtgctgc gaccgccgcg acaataagtg ctccctgttg
      421 gattttcatc agtccctcct ccgtggttga gattcgccgg ttgtatgaat gatgataaca
      481 agaagtccct aagacttgag acttggttca ccttacacag gcgatttttc cgtgtcaaga
      541 aggccgggcc gcctcatcca cgagacccag aactttctgc aagcctttga aatctttttc
      601 caccgggcag gcaaaatccc ggctccattc ccaccacgat ccgggatagt tgcgcacctt
      661 gcgataaccg gcaagcttca gaacgaaata gagccaggcc gagcggactc cgccggtgca
      721 atagcacacc acggcttgat ccgggtgaag acctttctcg tccagactcg ccttgatggc
      781 ctcgatgtcc ttgacggtcg cgttgggttg caaaaatccg ttccatccta catgcattgc
      841 cgaaggaatg tgcccgggac gcggaatccc cgagacttct ttaccgagat attcttcgag
      901 gctgcgggca tcgatgatca ctgtgcctgg aggtggttgc ttcacaagct gcttcacttc
      961 gtccttgttg gccatcacct cggtcttcaa tcggaccgta aaattccctg gtttcggtcg
     1021 tacaggccca tgctcaaatg gacgcttttc ctccacccac ttgacccatc ctccgtcgag
     1081 aattcgtaga tgctgatgtc ccaaatactc caacatccag aacatgcgac cttcgtcgcc
     1141 ccaattgtcg aagggattgg agtaaatgac gatgtcgctg tcgtcattga ttcccagggc
     1201 acgaattttc tgctgcatgc ggccgatatc gggatcgagc aaacccttcg cgacggcctt
     1261 tggatcgctg tactcgtgcc aggtggaatt gatggcaccg ggaatgtggc cgccgaattc
     1321 ataggcggca cgcccgcgga cgtcgatgac aacgaggccg ggctgcccca attgtttctg
     1381 gagcgtctct gtatcgatca ataacggatg cgtcatagta ctcctcttgt tgcgaccagc
     1441 gctcagtgat tagctctctg ccctcacctt acagctcaac gactccggcg gaacgctgac
     1501 agcagcctgt tgcttgctct cacggctcaa tgctgccgct aggtttccgg tcaacggaaa
     1561 ctcccgcaca taaatctcga atcggtaggg atcattcgct gtcaatccgt acttcgtgcg
     1621 gagcatctcg tgctgcccat cggtcaggat atggtactgc acacgggcct tcagggccaa
     1681 gccctcagat tcagccggca tgcgatacgt aaaccgatac tcgcggctgg ctagcggcag
     1741 gagccggttg tcgtacaact ccacaatcgc cggctgccac atgatccatc gccccatggt
     1801 cgacttgtcc tgatgaacca cctgtttcgc gcgatcctcg accagaaact cgatcgtgaa
     1861 atgccggtcc ggatcccccg tcggaatctt gtgaccggcg ccggcgttga tcagcgtcag
     1921 cgtaaacgtc acctgatcgc ccgacttcgg gtgcgacggg tcggccttga tctgcaccgc
     1981 gacggctcgt ttgatcatgt ccggatcatg ccctccgcgc cagaggtgct ggcgtccacg
     2041 tcggatcggg ccattcgttg caaccggtcg gtcgatctcc ggcatgtgac agctctgaca
     2101 gatgaagccc cgctcccgca tgaagaactt gccttcgtat tcagcatagg taccgcaggg
     2161 acccgcatta tagaactggg cgggaccgga cacgacgttg tgacaccggt aacagatttg
     2221 cgtcgtgcga aagttaggat cgaatttcgt cggatggggg gccgctgaat cctcgaaggg
     2281 cccgtagatg atgccatcac gcacatgaca ggcggcacag gtgatggctt ccttttgata
     2341 ctccgcatcg tagtgagggt tcggctcctg aattgctttc tcgacgcgcc cgcgcggaat
     2401 ttccttgatg agcgtcggct gctggttctc cagtggagta tggcagttca aacagaccca
     2461 gatgttcttg tctttcttcc aataggcttg gaagaacgga tccgcgaagg cgtgagcatg
     2521 gatgctcgcc ttccactcat cataaatctc gcggtggcag gtaccacaag actcggcctt
     2581 gagactgtcg agtccggctg gaaccttttg atagggaatg gcgtgcgcgt agtcggatcg
     2641 caatccgaag atcacgaccg gcttaatttc ggtgtaataa acgtatccgg cgccggcggc
     2701 aagcactacg ccgagaacga ttttgagtcc tcggctcata cgttcaacag tgccgtgaga
     2761 tgggagtcaa cagaggccgc cagcgccgtc aggttatatc ccccttcaag cgtcgagaga
     2821 attcgcccgc cggcatatcg cttggcgatt ccagcgacga tcgcggtcag atccgcatac
     2881 cctgcgtcgg tcaattccat actcgcgagc gggtcgtctt tatgcgcatc aaagccggct
     2941 gagatcatga cgaagtccgg cttgaaagtg tccgctgccg gaaccagcgt cttgcgaaaa
     3001 acggtccggt agtcctcatc gccaccacct gcttccatcg gcacgttgat ggtgaatcct
     3061 tctccacctc cagtaccaca ctctgtccca cgtcccgtgc cgggatagtg gggatattgg
     3121 tgagtgctaa aaaacaggat cgaagaatcg tcttcaaagc tatgttgcgt gccgttccca
     3181 tgatgcacat cccaatcaac gatgagtatc cgtggaagcc cgtaccgctt ctgcgcatag
     3241 cgagccgcga tggcaatatt attgaacagg caaaatccca tcgcgcgacc tgcctcggca
     3301 tgatgacctg gtggccgtac cgcacagaac acatggtcaa cttggtgatt catgatcgca
     3361 tcgacagcgg caagtgcccc acctgctgcg agataggcag cagttaagga ccccggcgac
     3421 atggaggtat ccggatcgag agagattcgg ccctgtatcg gagcgtgctt cgcgagcgcc
     3481 gccacgtatc ctggctggtg gatctgtgtc acccactcgt cttcagctct tcgaggttca
     3541 attcgagtga gctgccggag cgtcccgctc tgttccagct gctgcatgat cgcgcgaagg
     3601 cgatgagggg actcgggatg tccctgcccc atgtcgtgtt cgagatagtg cggatcgtag
     3661 acgagtcccg tcttgcccat aaaacgctcc actggaatag ccagaaatcg tagcacgcga
     3721 cgaacagcat agacaatcag cggaaacgcg tgttacggtc tttcggagtt caccggaggg
     3781 cgggatgcct aatccacgta tcgagccgct aaagaaagtc ctgacgatcg atccaaatga
     3841 cgatgttgcc tggttcgggc tagggaaagc ctatatggag gacgggaatt ttgacgaggc
     3901 cgccaaggcc ttacaacaat gcattacggt caagcccagc tattccgctg cctactttgc
     3961 gcttgctcaa tcgctccaca aactgggacg catcgacgaa tgtcgcgcag tttgcaccag
     4021 cggtatcgat gtttctacca gaaacggcga cgccatggtg accaagaacc tcgaggcgct
     4081 taccagctcg ctgcctacct agtcacac
//
LOCUS       DATDNW010000003         8485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1093321, whole genome shotgun sequence.
ACCESSION   DATDNW010000003 DATDNW010000000
VERSION     DATDNW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093321"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="ffh"
                     /locus_tag="VI359_00050"
     CDS             complement(<1..1158)
                     /gene="ffh"
                     /locus_tag="VI359_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY6972669.1"
                     /translation="MLDSLSEKFEKILKKLRGSGVLTEQDVAEALKEVRLALLEADVN
                     FKIVKDFLERVRVKALGQEVLQSLTPGHQVVKIVWDELRVMMGQERAGLALVSSPPTV
                     VMMVGLQGAGKTTTCGKLARLFKGQGRRVLLVAADPRRPAAGEQLASLGCELQIDVYR
                     FDQVHASQADVVRICQAGVERGRDQGYDLVVLDTGGRLHIDDELMEELVAVKNAVHPH
                     EVLLVADAMTGQDAVTMATQFDQRVGLTGVILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GVGEKLDALEPFHPDRIASRILGMGDVLSLIEKAQETFTREQAEEAQKRLTSNTFTLA
                     DFRDQLGQVNRLGSLDQILGMLPGGQKLKGAMEGQLPEREMKRVAAMIDSMT"
     gene            1383..1643
                     /locus_tag="VI359_00055"
     CDS             1383..1643
                     /locus_tag="VI359_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEY6972670.1"
                     /translation="MRKSPFLLLVFVLIGGLLGGVLGEILRVMAPQGTIQAIFATNFT
                     PGISPPLTIDLILVKFTIGFSLKINLLSLLGMFLGVYLYKNV"
     gene            complement(1640..3649)
                     /gene="uvrB"
                     /locus_tag="VI359_00060"
     CDS             complement(1640..3649)
                     /gene="uvrB"
                     /locus_tag="VI359_00060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY6972671.1"
                     /translation="MPSFRLEAPFAPCGDQGPAIDQLAAGVRAGTKHQVLLGVTGSGK
                     TFTMANLIERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVVIVSSVSCIYGLGSPEVYHGMVVYL
                     EEGMEIRREKILAKLVEIQYARNDIDFHRGTFRARGDVIEIFPASNEAVSVRIELFGD
                     VVDAIHEIDPLTGKSLKKLPKVTVYPNTHYLIAPDRYERAITGIEEELDERVAYFKKQ
                     NQLVEAQRIAQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGEPPPTLIDYFPKDFLL
                     IVDESHATVPQVGGMYEGDYSRKKTLVEYGFRLPSAVDNRPLKFAEFERCLNQVVYVS
                     ATPGPYELEHAREHVIEQIIRPTGLMDPVIDVRPAKGQVDHLLGEVQAEVAQGGRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIVRDLRRGVFDVLVGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARHVRGRVIFYGDVITDSMQLAIDE
                     TARRRRIQAEYNRVHGIVPESIRKEIPSLEYATAEMDYVQLDLAAESQPVYAKDEAVE
                     ETIHRLELEMKAAAKELEFERAAALRNRIRDLRIRELALKDQVSD"
     gene            complement(3674..4981)
                     /locus_tag="VI359_00065"
     CDS             complement(3674..4981)
                     /locus_tag="VI359_00065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6972672.1"
                     /translation="MSTFAEMSLPLFLAQRLQHAGMINPTPVQTAAIPPALEGRDILA
                     QAKTGSGKTLAFLLPLIERALRCELLSFGSGQFPKTGAGPIGGPKFLVLAPTRELALQ
                     IETELRKYAPAALASLAVYGGTPIERHYRALKRPPMVVVGTPGRLLDLAGSGHLRLGA
                     VTFLVMDEADQMLDRGFLPDIKRILNLLPKERQTLLFSATFASEIQTLAQSMQRDPVR
                     VSVDPGLNSPTSIVHAYYVVPAEQSRMQLVHTLLQTVKPGERSMVFCDQKYKVRRLAA
                     RLGGEPASVGSLTGNHSQAQRERTLGAFRTGRLRSLVATDVAARGLDIPDVSQVIHYE
                     LPANPNSYVHRSGRTGRAERQGSTFLILSQAEEREYLHMVRQLRIKTTKLPLPTLATL
                     PPAAALTNEDRPARHPRPQHRPDRGPRGFQEKEGRLSSSRYRW"
     gene            complement(5130..5429)
                     /locus_tag="VI359_00070"
     CDS             complement(5130..5429)
                     /locus_tag="VI359_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6972673.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAAHGTVASARIITDKFTGQSR
                     GFGFVEMSADNEAQAAITALNGTQLGGRTLTVNEARPQEPRSGGGGRGGFGGGRR"
     gene            complement(5695..6450)
                     /locus_tag="VI359_00075"
     CDS             complement(5695..6450)
                     /locus_tag="VI359_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972674.1"
                     /translation="MVAAFVLAMMVCERVTLDPNAQALQAESQTDKETNFKRQALGGL
                     FNQWTFDTQKPDEPPSGFSPLNLGEGPAAVWDIAADAEAPTKPNVVRGSSSCQESTCY
                     RLMVADKFEYEYPDITVRIQLPSQGVVGAGGVVLGVKDAENFYAVSVDLVGQTLEVIR
                     VIDGKESVLGRVPIKPKAVAWHTLRVQRNTIISKDFMETFFDGQLAASVEDQTLGLGR
                     IGLLLRGKTTLSFDSLHAVPLFSQRPLSPPAAY"
     gene            complement(6515..6769)
                     /locus_tag="VI359_00080"
     CDS             complement(6515..6769)
                     /locus_tag="VI359_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972675.1"
                     /translation="MGANDQSKGLYDHLYRRFFESRDRHQGYEFQHAPGGSAPTPVVS
                     FSRKLRWWTWDRWQRRKKILAERDRLQREIVEIKNHSSHS"
     gene            6940..>8485
                     /locus_tag="VI359_00085"
     CDS             6940..>8485
                     /locus_tag="VI359_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6972676.1"
                     /translation="MLVKTEFPVLNLALIPVIAFVFAVLPPPVHSLDGPGALSSTIGQ
                     ISGERMMADVAALSGIAFNGRQTGTADDLRSAQWVANRFRTSQLSLVAIPTDRLDTPD
                     GSGEGLEPPTGFMSTPHKAVIIAPGPVLQVSTARVPLNQELGTDFLPVLDSPSSHVRA
                     PIVFVGYGIVDSAIGMNEYDGVDVHNCIVLFLRGKPDHYKGTITHADKIRIAAQKGAI
                     AYLTVTGPVLSAYEMRRGVTGRPSAFYNLAADADTLPGAWISTSQVEYILAGTDPSKS
                     RHLRTIQEQLNASPTARSFRTDYFGVLEWEVRQEHGLLINVVATLPGTDPKMANEAIV
                     IGAHRDHFGKQAGLLFPGADDNASGTAVMLEVARTIAKLPIKPKRTLLFLSFSGEEQG
                     LLGSRLYVGKPVIPLGHTKAMINIDHAGIGNGRLTVGATGLDKTLVQEAGQSAGLADK
                     VDVFGFFPGGDHVPFKEAGIPTVTVVSGGTHLHFHQPTDTVETLNQEILVSAARYVLA
                     LAWQLAN"
BASE COUNT         1770 a         2581 c         2280 g         1854 t
ORIGIN      
        1 agtcatcgaa tcgatcatcg cggccacccg cttcatctct cgttccggca gctgcccttc
       61 catcgccccc ttgagctttt ggccgcccgg cagcatcccc agaatctggt ccaaggagcc
      121 gaggcgattg acctgcccca actgatctcg aaagtctgcc agcgtaaagg tgttgctggt
      181 cagccgcttc tgggcttcct ccgcctgttc gcgggtaaac gtctcttggg ccttttcgat
      241 cagcgagagg acatcgccca tcccaagaat gcgcgaggcg atccgatcgg ggtgaaacgg
      301 ctcgagcgca tcgagttttt caccgacgcc gaggaacttg atcggtttac cagtcaccgc
      361 ccggatggac aacaccgcac cgccacgggc atcgccttca accttggtca gaatgactcc
      421 ggtgagtccg actcgttggt cgaactgggt cgccattgtc acggcatcct ggccggtcat
      481 ggcatcggcc acgagcaaca cttcgtgggg atggaccgcg ttttttaccg cgactaattc
      541 ctccatcaac tcgtcgtcga tgtggagtcg ccccccggta tccaacacca cgaggtcgta
      601 cccttgatca cgtcctcgtt ccacgccagc ctggcagata cggaccacgt ccgcttgaga
      661 tgcgtggacc tgatcaaacc ggtagacgtc aatctggagt tcgcacccta aacttgccaa
      721 ctgttcaccg gctgccggcc gccgaggatc cgctgcgacc aggagcacgc gtctcccctg
      781 tcctttgaac aatctggcga gcttcccgca cgtcgtcgtt ttgccagctc cctgaagccc
      841 caccatcatc accaccgtgg gcgggctgga cacgagagcc aggccggctc gttcctggcc
      901 catcatcacc cgcagctcgt cccagacgat cttgaccacc tggtgaccgg gagtcaggct
      961 ctgaaggacc tcctgaccga gcgccttgac tcggactcgt tcgaggaagt ctttgacgat
     1021 tttgaaattg acgtcggctt ccagcagtgc gaggcggacc tctttcaacg cttccgcgac
     1081 gtcctgttcc gtcagcaccc cggaaccgcg gagcttcttc agaattttct cgaatttctc
     1141 gcttaaacta tccagcatgg gctcacaaag aaaaaggcca tcgaagcctt acacccagat
     1201 ctccgatcgc ctcgaaaaat ctagcaaaag agcatcgggc agtctaaagg acgagggggg
     1261 agaagtcaag ggacgatccg acacgagaag accgcgcaag ttgttgacag actagagacc
     1321 tttcgctatg gtcctgatga atgtcgtcgt gagacaacca gtcaaattat gagatcggaa
     1381 tcgtgagaaa atcccctttc ctcctcctcg tttttgtcct gatcggcgga ttactgggcg
     1441 gggtgctggg agaaatcctg cgggtcatgg cgccacaagg caccattcaa gcgatatttg
     1501 ccacgaactt tactcccgga atcagccccc cgctgaccat cgatctcatt ctggtcaagt
     1561 tcacgatcgg cttcagcttg aagatcaatc tcctgagcct gctgggcatg ttcctgggcg
     1621 tctacctcta caagaacgtc taatcgctca cctgatcctt taacgcgagc tctctgattc
     1681 ttaagtctcg gatccgattg cgcagcgctg ccgctcgttc aaactccaac tcttttgctg
     1741 cggctttcat ctccagttcc agccgatgaa tcgtttcctc gactgcctcg tctttcgcat
     1801 agaccggttg ggattcggct gccagatcca gctggacata gtccatttcc gcggtggcgt
     1861 attcgagcga cggaatctcc tttctgatac tctcaggcac gatcccgtgg acccggttgt
     1921 actcggcctg gatccgacga cgacgtgccg tctcgtcgat ggccaattgc attgagtcgg
     1981 tgatcacgtc gccgtagaag atgacgcgac cccgtacatg tcgtgccgct cggcccgcgg
     2041 tctgaatcaa ggagcgatgc gagcgaagat agccttcttt gtccgcatcg agaatggcca
     2101 cgaggctcac ctcgggtaga tccagccctt cccgcaacaa attgattccg accagaacgt
     2161 cgaagacccc acggcgcaag tcccgcacaa tctcggctcg ttccaacgtc ttgatgtcgg
     2221 aatggaggta ccgcaccttg acgccgaggt cgtgatagta ctcggtcaaa tcctcggcca
     2281 tgcgcttcgt gagcgtggtg acgagcaccc gccctccctg cgccacctcc gcttgtacct
     2341 cgccaaggag gtgatccacc tgcccttttg ccggccgcac gtcgatcacg ggatccatga
     2401 gcccggtcgg tcgaataatc tgttcaatta catgctcacg cgcatgctcc agctcgtagg
     2461 gacctggcgt cgcagagaca tacaccacct gattcagaca gcgttcaaat tccgcaaatt
     2521 tcaacggccg gttatccacc gcggatggga ggcggaatcc atattcaacc agggtctttt
     2581 tgcgcgaata gtcaccttcg tacatgccgc cgacttgtgg aactgtcgcg tgtgattcat
     2641 caactatcaa cagaaaatct tttgggaaat aatcgatcag cgtgggaggc ggttcgccgg
     2701 gcgcccgccc gctgaggtgg cgggaataat tctcgatgcc gtggcagtag cccatggcgc
     2761 ggatcatttc caggtcaaac ttcgtccgct gcgcgatacg ctgcgcttcc accagttgat
     2821 tctgcttctt gaagtacgcc acgcgctcgt cgagctcttc ttcaatgccc gtgatggccc
     2881 gttcataccg atcgggcgcg atgaggtagt gcgtgttcgg atagaccgtg actttgggaa
     2941 gcttcttcag cgactttccc gtcagcggat cgatctcatg aatcgcgtcg acgacatccc
     3001 cgaatagttc gatccgtacc gacacggctt cgttcgaggc cgggaaaatt tcaatgacgt
     3061 cgccgcgcgc gcggaaggtt ccgcgatgga aatcgatatc gttgcgcgcg tactgaatct
     3121 cgaccagctt cgcgagaatc ttctcccgcc tgatctccat cccctcttca agatagacca
     3181 ccataccgtg atatacctcg ggcgatccga gcccatagat gcacgagacc gacgacacga
     3241 tcaccacgtc gttgcgctgc aggagcgagg ttgtcgccgc atggcgcatc tggtcgatcg
     3301 cgtcgttgat cgacgcatct ttggcaatgt acgtgtccga ttgcggaata taggcttcgg
     3361 gctgatagta atcgtagtag ctgatgaagt attcgactgc gttgtggggg aagaactgct
     3421 tgaattcctg gtacagctgg ccggcgagcg tcttgttgtg caccaggacg agcgtgggtt
     3481 tttgcacacg ctcgatcagg ttggccatcg tgaatgtttt gccggagcct gtgactccca
     3541 aaagcacttg atgtttcgtc cccgcccgaa ccccggcggc cagctggtca atcgctggcc
     3601 cctggtctcc acaaggtgca aacggggctt ctaggcgaaa cgacggcatg gcggcgatat
     3661 tcccgccgtc atcttaccat cggtacctcg agcttgatag ccggccttct ttttcctgaa
     3721 atcctctcgg cccacgatct ggtctgtgct gaggccgagg atggcgcgcc ggtctgtctt
     3781 cgttcgtcag cgcggcagcc ggtggcaagg tcgcgagtgt gggaagaggc aatttcgttg
     3841 tcttgatgcg caactgacgc accatgtgca aatactctcg ctcttcggct tgagacagga
     3901 tcaggaaggt cgatccctgc cgctccgctc gtccggtccg accgctgcgg tgcacgtatg
     3961 aattcgggtt ggcaggcaat tcatagtgaa tgacctgcga cacatcgggt atgtccaacc
     4021 ctctcgctgc cacatcggtg gcgacgagtg aacggagccg acctgttcga aacgcaccga
     4081 gcgtccgttc ccgttgtgcc tggctatgat tgccggtcag cgaaccgacc gacgccggtt
     4141 caccgcccag tcgcgccgcc aagcggcgta ctttgtactt ctgatcgcaa aacaccatcg
     4201 accgttctcc cggcttcacc gtctggagca atgtatgaac cagttgcatc ctcgactgct
     4261 cggccggcac gacatagtag gcatgcacga tcgacgtcgg gctgttcaga ccggggtcga
     4321 cggagacgcg aaccggatcg cgctgcatgc tctgcgcgag ggtctggatc tctgacgcga
     4381 acgtcgccga gaacaacaat gtctggcgct ccttcgggag cagattcagg attcgtttga
     4441 tgtccggcaa gaaccctcga tccaacattt gatccgcctc atccatcacc agaaacgtga
     4501 cggcgccaag acgcaggtga ccggatccgg ccagatccaa taaacgaccg ggggtgccca
     4561 ccacgaccat cggcgggcgt ttcagcgcac ggtaatggcg ctcgatcggt gtgcccccat
     4621 agacggccag ggacgcgaga gcagccggtg cgtacttgcg caactcggtt tcaatctgca
     4681 gcgcgagttc cctggttggt gccaacacca gaaactttgg tccaccgatg ggaccggctc
     4741 ctgtcttggg aaattgcccg gatccgaacg acagtaactc gcagcgcagc gcccgctcga
     4801 tcagcggcag caagaaggcc agcgtttttc cgctccccgt cttggcttgg gcgagaatat
     4861 cgcggccttc gagggccggt gggatcgcag ccgtttggac aggggtggga ttgatcatcc
     4921 cagcatgctg caggcgctgg gcaaggaaca ggggtagact catttcagca aacgtcgaca
     4981 taacactcct tgtgttgtga acttcctggg actgcacggt tcggccgaat cagacggccg
     5041 agtgaggcat cgaaacgatg ggcaattcta ggaaggaaat agcggggcca ccatgacggt
     5101 ggccccaatg gttttctttt cgttgcggtt tatcgccgcc cgccaccgaa tccgccgcgg
     5161 cctccgccgc cggaacgtgg ctcctgggga cgtgcttcgt tgaccgtcaa cgtgcgacca
     5221 cccaactggg tgccgttcag cgccgtgatt gccgcctgcg cttcgttatc cgcggacatc
     5281 tccacgaatc caaagccacg ggactggccg gtaaacttat cagtgatgat ccgggctgac
     5341 gccactgtcc cgtgtgcagc aaacagatcg ctcagctgct gctcggttgt ggaatacggc
     5401 aaacccccaa cataaatctt cgaacccatt gggttcctcc tttgagatat tggtgttgtc
     5461 ttggactcga gaacgaagtg aggaaggaat gggccgaaga cgcaaacacg gtggcggctt
     5521 agctctggct tccgatcaga ttcccgggca cgaccaaaac aacatccgtt caggccttgt
     5581 cactttctgt tgcatcacta ccaggccttt cgcagtgaca cacggacgca ctctacctta
     5641 caacctcgca aaaatcaagc acgagacgcg actcccacag accatccgac acttctagta
     5701 ggcagcaggg ggagataagg gccgctgaga aaagagcgga accgcgtgca agctgtcgaa
     5761 agataaggtt gtcttccctc gaagcagcaa gccgattcga cccaggccaa gggtctgatc
     5821 ttccaccgag gctgccagct gcccgtcaaa gaacgtctcc ataaaatcct tactgataat
     5881 cgtattgcgt tggacccgaa gggtatgcca cgcgaccgcc ttcggtttga tcgggactcg
     5941 gcccaagacc gattccttcc cgtcgatcac acgaatgacc tcgagcgttt gtccaaccag
     6001 gtcaaccgac actgcataaa aattctccgc atctttgaca ccaagaacga cccctcctgc
     6061 tccaacaacc ccctgactcg gaagttgaat cctgacagta atgtcgggat attcatattc
     6121 gaacttgtct gccaccatca gccgataaca cgtactctct tggcaagacg aggatcccct
     6181 gaccacgttc ggtttggtcg gagcttcggc atcagcggca atatcccata ccgcagccgg
     6241 tccttctccg agattcaacg gcgagaaacc gctcggaggt tcatcaggct tctgcgtatc
     6301 gaacgtccat tggttaaaca atcctcctaa cgcctgccgt ttgaagtttg tctctttgtc
     6361 cgtttgactt tcagcctgca aagcctgggc attggggtcg agcgtcaccc gctcacagac
     6421 catcatggcc agaacgaacg ccgctaccac aacatggaat cgctgccccc tcaggcacgc
     6481 agaggtcttt cttaccgacg aatttcttga catgtcacga gtgtgaggag tggttcttga
     6541 tttcaacaat ttcccgctga agtcgatctc gttccgccag aattttcttc cgtcgttgcc
     6601 atcgatccca tgtccaccac cggagtttcc ggctgaacga cacaaccggc gttggtgcgc
     6661 tcccacccgg agcatgctgg aattcatatc cctgatggcg atcacgactt tcgaagaacc
     6721 gccgatacag atgatcgtat aagcccttgc tctggtcgtt ggcccccacg atgtctccct
     6781 tcactcatcc gcagtgcaac ggctcagact agccgtcttc cattcatgag gcggcatact
     6841 accgtggccg gaagcggctc tgcaaccggt ctccatcacc gttcaaacta tgttatatag
     6901 gctcctctgt gcactcggag acggcccggg ttgaagaaga tgcttgtcaa aacagaattt
     6961 ccggttctga acttagccct gattcccgtc atcgcctttg tgttcgcggt tctccctccc
     7021 cctgtccact cgctcgatgg acctggcgca ctgagctcaa cgatcggtca gatttcgggc
     7081 gaacgtatga tggccgatgt ggccgcattg agcgggatcg cattcaatgg ccggcaaacg
     7141 ggaactgccg atgacctgcg atcggcacaa tgggtggcca atcgattccg tacatcccaa
     7201 ctcagccttg tcgccattcc aacagatcga ctcgatactc cagatggttc tggagaaggg
     7261 ctggaaccac cgaccggctt catgtcgacc ccccacaagg ccgtcatcat cgcccctgga
     7321 ccggtccttc aagtctcgac tgcacgcgta ccgctcaacc aggagctcgg aacggacttt
     7381 ctgccggtgc tcgattctcc ttcctctcat gttcgtgcac cgatcgtatt cgttgggtac
     7441 ggaattgttg attcagccat cggcatgaat gagtacgacg gagtcgatgt gcacaactgc
     7501 atcgtactgt ttctcagagg caaacctgat cattacaagg gcacgatcac ccatgcggac
     7561 aagatacgga tcgccgccca gaaaggcgca attgcgtatc tgaccgtgac cggaccggtc
     7621 ctgagtgcct atgagatgcg gcgtggcgtc accggacggc ccagtgcctt ctacaatctt
     7681 gctgcagacg ctgacacgct gcctggcgcg tggatcagca cctcgcaagt ggaatacatc
     7741 ctcgcaggaa ctgatccttc caaatcgaga cacctgcgca caatccaaga acagctaaat
     7801 gcctctccta cagctcggtc ttttcgcaca gattacttcg gagtgctcga atgggaggta
     7861 cggcaggagc atggactctt gatcaatgtg gtggcaaccc tgcccggaac cgatccgaaa
     7921 atggcaaatg aggccatcgt gatcggcgct catcgggatc attttgggaa acaagccggc
     7981 ttgctctttc ccggcgctga tgacaacgcc tcaggaaccg ccgtcatgtt ggaggtcgcc
     8041 cggaccattg ccaagctacc gatcaaaccc aagcgcacgc tgctgtttct gtcgtttagc
     8101 ggagaagagc aaggcctgct cggatcgcgg ctctacgttg gaaaaccggt gattccactt
     8161 ggccacacca aagccatgat caacatcgat catgcgggaa tcggaaacgg aagattgacg
     8221 gtcggcgcca cagggttaga caagaccctc gtgcaggagg caggtcagtc agctggcctc
     8281 gctgataagg tggatgtgtt cgggttcttc ccgggcggcg accatgtgcc cttcaaagaa
     8341 gcggggattc ccactgtcac agtcgtgagt ggcggaacac atctgcattt tcatcaaccg
     8401 acggatacgg tcgagacact caatcaggag attcttgttt cagcggcccg ctatgtcctc
     8461 gcactcgcct ggcaactcgc caatg
//
LOCUS       DATDNW010000004         3989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_342317, whole genome shotgun sequence.
ACCESSION   DATDNW010000004 DATDNW010000000
VERSION     DATDNW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342317"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VI359_00090"
     CDS             complement(<1..635)
                     /locus_tag="VI359_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY6972677.1"
                     /translation="MPARVVITGLGIVSPIGIGVSEFWKAALAGRSGITAIKSFEPFP
                     MDGYRSRVAGQIHDFVPEQLLTGVLAERVDRYAQFALVAAKEALADSHLQMGRENPHR
                     VGVIVGAGMGGMVMGEREITQLYQQQKPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQALQCIRDGRADVVITVGADASITPLVFAGFCSLRAL"
     gene            complement(803..1894)
                     /gene="lptG"
                     /locus_tag="VI359_00095"
     CDS             complement(803..1894)
                     /gene="lptG"
                     /locus_tag="VI359_00095"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY6972678.1"
                     /translation="MTILFRYILREYARIFLMCFAGLMTIYLVIEFFEKVRRFLNYDA
                     SWIDVLAYFALKTPAISFQIAPLAVLMATLLTLGLLSRSHEITAMRSCGISLPWIASP
                     FLLFAAGVALILLSFSSTIIPLAADKAEDIKVTKIEKRPPAATLTIQQPWIRVGPYTL
                     MNVTSGAVGGTKIGGIRLFQFERGFRLVDLTEAEEARYGDQEWMLYRGRHRRFLPDGS
                     VSVVEFDQQPIALPLIPEDFTSRFGSDSENMTFRDIRSYTARLSHEGAQLSRLLTDYY
                     GRVAFPFVTIIMVLVGIAMSLRRSGVRGGSMAIGIGQSLAVGFCYWTTHSIAIALGRG
                     GALSPMMAGWAANMLFLSFALYLMLKVRY"
     gene            complement(1898..2989)
                     /gene="lptF"
                     /locus_tag="VI359_00100"
     CDS             complement(1898..2989)
                     /gene="lptF"
                     /locus_tag="VI359_00100"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY6972679.1"
                     /translation="MFLRTLLDRYILSELLSPFGLSLGVLCFVMLTKELLRLVELLVA
                     KGVGLLAVIKVFAHLLPSFLVLTLPIAGLIASITAFGRLSYDKELVAMRAAGLSLFRL
                     SRSVVLFAMLVFSLTLLLAQWGQPWSSLNLKKVALNLLRDQLVMALERGTFNEPIPRL
                     VIFVTDAQDPGVTPGIFISDERNVEDPRIIVATQYQVLMEPSNNHVALRLQNGVIHSR
                     PDQANQYQETSFENYDLKLSLSQSGYSTAEERPSYESIVARLDQSAWHDPTALRRLIE
                     YYKDLAFPTAALIFCILGVPVGIVSKRSGRIGGFAVGVLIVIIYYVLNVGCEFLVTTL
                     VIPPFAGAWIPNAMFVLITLWWFRAVSRQ"
     gene            3287..3508
                     /locus_tag="VI359_00105"
     CDS             3287..3508
                     /locus_tag="VI359_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY6972680.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGDGFKTLKE
                     GESVEFDIVQGAKGPQAANVLKMSVSASQ"
     gene            3651..>3989
                     /locus_tag="VI359_00110"
     CDS             3651..>3989
                     /locus_tag="VI359_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972681.1"
                     /translation="MGIDRGKLLHNAQLFASRGQFDAAIAEWKKLIAESPADGSLHNS
                     IGELHLKRNANAEAVAAFLQAASAFRAEGATLKAIATYKKILKIDSSRYEVYRHLGDL
                     NAERGLLSSAV"
BASE COUNT         1020 a         1038 c         1108 g          823 t
ORIGIN      
        1 agagcgcgga gagaacagaa tccggcgaat accaacggcg taatgctggc atcggcgccg
       61 actgtgatca cgacgtctgc acgaccgtcg cgaatgcact gaagcgcttg cccgagcgcg
      121 tgggcgctcg atgagcaggc cgtagaaata gtcaggttag gacccttggc tccataggcc
      181 attgcgacga tgccggaggc agagttcaac gtaatcaccg ggatgaagtt cggatggacc
      241 cgatgaggtt tctgttgttg gtacagctgg gtgatttctc gttctcccat caccatgccg
      301 cccatgcctg ctcctacgat cacaccgaca cgatgaggat tttcccgtcc catctgaaga
      361 tgagaatccg ccagtgcctc tttggctgca acgagtgcga attgcgcgta ccggtcaact
      421 cgttcggcaa gcacaccagt taacagctgt tcaggaacaa aatcatggat ctgaccggct
      481 acacgtgacc gataaccatc catgggaaat ggctcaaaag acttgatcgc ggtgattcct
      541 gagcggccag ccaacgcggc cttccagaat tctgagacac cgatcccgat cggggagaca
      601 atacccagtc cagtaatgac tactcgagcc ggcatgagag accaatcctt tccaaaagaa
      661 acagcttcag tacttaccgg aagccggtca tacagtcaac cggtcagatg aggttcaggg
      721 aagcaggctg ggagatcgtt ccgatgccac aagggacagc ttcgatgatg aaagcacagc
      781 agaggttttc cccatgcttt tgctaataac gtactttgag catcagatag agcgcaaaac
      841 tcaaaaagag catattggct gcccagccgg ccatcatcgg cgacaatgcc ccaccgcggc
      901 cgagggcgat agcgatcgag tgggtagtcc aatagcaaaa ccctaccgcg agcgactggc
      961 caatcccaat cgccatgctt cccccgcgga caccgcttcg ccggagactc atggcgatcc
     1021 cgacgagaac catgatgatg gttacgaacg gaaacgcaac acgaccatag tagtccgtga
     1081 gcaatcgaga gagctgtgcg ccttcgtgag aaaggcgtgc ggtataggaa cgaatgtcgc
     1141 gaaaggtcat gttctcagaa tcacttccga atcgcgaggt gaaatcttct ggaatcagcg
     1201 gcaacgcaat cggctgctga tcgaactcga cgactgagac ggagccatca ggaagaaacc
     1261 gtctgtgccg tccccgatag agcatccatt cctgatcgcc ataacgagct tcttcggctt
     1321 cggtcaaatc gaccagacga aaccctcgct cgaactgaaa aaggcgaatg cctccaatct
     1381 ttgttccacc aacggccccg ctggtcacat tcatcagcgt gtagggtcct accctgatcc
     1441 aaggctgttg aattgtcagt gtcgcagccg gtggcctttt ttcgatcttc gtgaccttaa
     1501 tatcttcggc tttgtcagca gctaagggaa tgatagttga gctaaacgag aggagaatca
     1561 gggcaacgcc cgcggcgaac aacaggaagg gggaagcgat ccagggcaaa ctgattccgc
     1621 aactgcgcat agcagtgatt tcgtggctgc gtgacagaag gcctagggtc aacagcgtgg
     1681 ccatcaatac cgcgagcgga gcaatttgaa aggaaatggc cggcgtcttc agcgcaaagt
     1741 aagcgagaac atcgatccag ctggcgtcat aatttaaaaa gcgacggact ttttcaaaaa
     1801 actcgatgac gagatagatc gtcatcagcc cggcgaagca catcaaaaat attctggcat
     1861 actctcgcag aatatatcgg aagaggatcg tcataaccta ttgacggctg actgcacgaa
     1921 accaccacaa cgtgatcagg acaaacatgg cgttcgggat ccacgctccg gcaaagggag
     1981 gaataaccag agtcgtcacc aagaattcgc agcccacgtt caagacatag tagatgatca
     2041 cgatgagaac accgacagcg aacccgccga tccgtcctga ccgcttcgag actattccga
     2101 ccggtacccc caggatacag aagatgagcg cggcggtcgg aaatgccaag tctttgtaat
     2161 attcaatgag acgtcgtagg gcggtggggt cgtgccaggc agattggtcc agtcgagcca
     2221 caatcgattc gtaggacggg cgctcctccg ccgtcgaata tccactttgg ctcagcgaca
     2281 gtttgaggtc gtagttctca aaagaggtct cttggtattg gttcgcttgg tcaggacgac
     2341 tgtggatgac tccattctgc aaacggagcg ccacatggtt gttcgagggc tccataagaa
     2401 cctgatattg tgtcgcgaca ataattctgg gatcttcgac gttacgttcg tctgagataa
     2461 agataccggg cgtcacacca ggatcttggg catcggtgac aaagatcacc aatcgcggga
     2521 taggttcatt gaatgtgcct cgttccaagg ccatcaccaa ttgatcccgt aagagattta
     2581 gcgccacctt ttttaaattg agcgaactcc agggctgtcc ccattgggcc aagagcaacg
     2641 tcaggctgaa taccagcatc gcaaagagaa cgaccgaacg agagagccgg aacaggctca
     2701 agccggcggc ccgcatagcg acgagttctt tgtcatagga caagcggcca aatgccgtaa
     2761 tggatgcgat gagtcctgcg atgggcaacg tcagaacgag aaaagaaggg agtagatggg
     2821 caaatacttt tatgaccgcg agcaagccga cgcccttcgc aacgagcagc tcaaccaaac
     2881 gaagtaactc tttggttaac atgacgaaac agagcactcc gagactgagc ccgaaggggg
     2941 agagaagctc cgagaggatg tagcgatcga gcagcgtgcg taggaacatc tgagggatca
     3001 cactcctgtt ggtcagcgtc actataggcg agtgagcggg tcttgtctag aaaaccgctg
     3061 actgcggatg ggtcacaaag aagtgcttga caggtgtgaa tcgctatgtt acatgcaccg
     3121 cgattttcaa ttgagatggg ttttgtgaac gcttcacatc tcctccagcg agcccgtcgc
     3181 ccggtcttct tctcgcactg tcattctttc tttatcgcca gtcaacatca gggggtttgg
     3241 gccggtcacg taacatcgcg tgaccgttga ggaaaggagg gtttttgtga agacgaaagg
     3301 tactgtgaag tggttcaacg atcgaaaggg gtttggattt attcgtcttg acagcgggga
     3361 ggatgtgttt gtccattact cggcgctcca aggcgacgga ttcaagacac tcaaagaagg
     3421 agagagcgtc gagttcgata tcgtccaagg ggcgaaggga ccgcaggcgg ctaatgtgtt
     3481 gaagatgtcc gtctcggcgt cccaatagac aagtcctttt caaggtgtgt gcgaaggtct
     3541 gctgcactgt tggtggagca gaccttcgtc gtttgcaggg tttctgttta cccatttcgt
     3601 tgacaaaacc cattgatact gttagctttt gttcgttatg gaggtctgcc ttgggcattg
     3661 accgcgggaa gctattgcat aacgcccagc tctttgcctc gcgcggccaa ttcgacgcgg
     3721 ccatagccga gtggaagaag ctcatagcgg agtcaccggc tgacgggagc ctgcataact
     3781 ccatcggaga gctacatctc aagcgcaacg cgaacgcaga agcggtggcg gccttcctac
     3841 aggccgcaag cgcgttccgc gcggaaggcg ccaccctcaa agcgatcgcg acctacaaga
     3901 aaatcctcaa gatcgattcg tctcgttacg aagtctatcg gcatctcggc gatctcaatg
     3961 ccgagcgagg cctgctaagc agcgcggtt
//
LOCUS       DATDNW010000005         6447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_479045, whole genome shotgun sequence.
ACCESSION   DATDNW010000005 DATDNW010000000
VERSION     DATDNW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479045"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VI359_00115"
     CDS             <1..952
                     /locus_tag="VI359_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY6972682.1"
                     /translation="QAHRQPGSLVKPFVYLAALEAAREGNAGAFTPASLLADEPVTFE
                     SEKGLWAPLNYDRQFRGHVTLRQALEQSLNVPAVRVAHAVGTKPIVQLLHRLGVNSPI
                     SEDLSMVLGSSSVSLLEITSAFGGIANGGVAVHPTAVRTARDRQGDSVWSGVPDRSQA
                     VSPQSAYVLTSLLEGVIERGTATKAKVMGLQGAVAGKTGTTDGYRDAWFVGYSPDLVI
                     GVWVGFDDERPIGLTGAQAALPIWIDIARRVIPSQTPTFVMPTGVVTRTIDPKTGQLA
                     TSQCPEQVSEVFVEGTAPSVYCEIHGGGIWERFRRTFGFS"
     gene            1012..1455
                     /locus_tag="VI359_00120"
     CDS             1012..1455
                     /locus_tag="VI359_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6972683.1"
                     /translation="MKKPGFTGDDNITLLAKGVELKGEIRVEGTVRIDGSLEGDIQTK
                     GQVIVGEDGLVQGTISAGTIISSGRIKATVTAIERIQLLKTATLIGEVHTPILMMEEG
                     AKLQGTTDMGVAAWPEELPKLPGTVRNLSARRTKQVVAIDHEPRT"
     gene            complement(1541..2140)
                     /locus_tag="VI359_00125"
     CDS             complement(1541..2140)
                     /locus_tag="VI359_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6972684.1"
                     /translation="MDRVGHSISTASIFFLVLASVTSATPPDVKREAPVASIPAEKVA
                     AYVHAVIQADRTIYTSQIVNRMQEKHIVTAAEHWEQDNALPLPAQFLQQSGRAVAEGG
                     SGIRFRLIGLSPIYQRNAPATEFERKALETLARQSDRPVAGIVTSGQKRYYQAIYPDR
                     AVAAACVTCHNAHPLSPKRDFKVNDVMGGIAITIPLDEK"
     gene            complement(2086..4620)
                     /locus_tag="VI359_00130"
     CDS             complement(2086..4620)
                     /locus_tag="VI359_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6972685.1"
                     /translation="MGSSHPSRTGPRKGWGLQPKLILSMLMVGVLPLLVGLGMAFWQG
                     SHEIQEVNGESFKALAAETARKLDILVAEEVARTSRIARDPVIVAELEHRRDVLNTRR
                     DQESLRAVRATINEDAIRWEAKDPAIVKTVTGGPLSNLLRSYYTGSQSEQEQLLPQVV
                     RAATKMLFVTDVQGSLVASMTTRPKYSNGSAPWWIGAYHKGIGQLYIEDVSYHEQAGT
                     YAFTLSLPIMDSLHYEVVGVLHRVIDAKEFFSPSIQPIRFGKTGHVMLIDSRGIVMSC
                     PILPTGVSISDLSLIPLVTPFHPGWVAAPSDGHGGRSTSIIGFAPLPETSRATNGRLE
                     EGSWNTFVWQSSDELFAPIRHLFTWMTIFGLVAVCLLVALGYLAANRIVLPIRQLQKA
                     AESIGRGELRQSIQIQTGDELEALAEDFNRMNKQLEAAFAGLTDQVALKTQEVQSLER
                     STDQILNAVPTPILMVDAQAQVQYVNHAAQESLGLSPSLSPSTSLFDILPLDAPAQQR
                     LRKEFQERDSTGLPTMERAMETSTDEPRDPLAPTLTGGRTNGRSELRIEQRVYHYEWF
                     RTDGRPGEGPRVGLVLRDTTDESRLQDQLIQAEKSGSLGILTAGIGHELNNPLFGILG
                     LGEAIQEEANLDHVKAHARDIVQHGRRMAAIIRDFTGMAARDTGTRLIPVDVHLELDQ
                     ALAALQTTHDLSRFEIHKSYACRDRVSASPEQLRQAFLNILTNAVQAMPGPGTLRVAT
                     TADNKMVTITIEDSGHGIPSQHLSKVFDPFFTMKGQGEGSGLGLTVARRIIRKFGGDI
                     RIENLDRGGVCCTISLPVIQSSLGPQEASWTASGIPSAPHPSSSSS"
     gene            complement(4626..5669)
                     /locus_tag="VI359_00135"
     CDS             complement(4626..5669)
                     /locus_tag="VI359_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6972686.1"
                     /translation="MDIVPPTKVAIVGAGRGGRALLDLFHQISSIEVIGIADRDPRAP
                     GLQRAREYEIPVTTDVAELIRNPAISVILDVTGDPGMESLLRIQKPATTELLSGSASR
                     LLWQIIQYESALQAELMQAEKLAGIGSFATGIAHDINNPLQLIMGLAENLIEERDLPT
                     IREQAREIVDAVKRTSAICQDLTRYSRQSSASEASLVSVNGKLDEALKIARYAARLHD
                     IEIVKHYTQGAAVRGNPDELLHMFVNLITNAVHAMEHVGGTLTLETRVMADTVDVRIS
                     DTGCGIAPELREKIFEPFFTTKPPGKGTGLGLYNARNIVHEMHGNIAVDSEVDKGSTF
                     TLTFLTQETVSHG"
     gene            complement(5771..6205)
                     /locus_tag="VI359_00140"
     CDS             complement(5771..6205)
                     /locus_tag="VI359_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6972687.1"
                     /translation="MTEFKSGFFTGGEACNGRVLVVDDEPDIRKVVRMTLQKAGYEVI
                     EAENGEKAIQAINEGENRLLLDVVICDIRMPKINGAEAIAYFRSNYPRVPLIVLTGFP
                     DTDMATSFLRQGVVDYLVKPVEGEKLKEAVRRAMEERELAKL"
BASE COUNT         1288 a         1780 c         1952 g         1427 t
ORIGIN      
        1 gcaagcgcat cgccaaccag gctcgctggt caaaccgttc gtctatcttg cggcgctcga
       61 ggcggcacgc gaaggaaatg ccggagcgtt cacaccggcg agtctcttgg cggacgagcc
      121 ggtcacgttt gagtcggaaa aagggctatg ggcgccactc aactatgatc gtcaattccg
      181 cggtcatgtc acgttgcgtc aagccttgga gcaatccctc aatgtaccgg ctgtcagggt
      241 cgcccacgct gtcggcacca agccgatcgt tcagcttctg caccgactcg gagtgaatag
      301 cccgatctca gaggacctgt ccatggtctt ggggagttct tccgtctcct tattggagat
      361 cacgtcggcg ttcggcggga ttgcgaacgg aggggtggcg gttcatccga cggcggtccg
      421 gacggcaagg gatcgacaag gagattccgt gtggagcggg gtaccggatc gctcgcaagc
      481 tgtgtccccc cagagtgcgt acgtgctgac gtcgttgctt gaaggggtga ttgagcgagg
      541 aacggctacc aaggccaaag tcatgggctt gcaaggcgcg gtggcaggaa aaaccgggac
      601 gaccgatggg tatcgagatg catggttcgt gggatattcg ccggacctcg tcatcggcgt
      661 ctgggtgggc ttcgacgatg aacgtcctat cggtctcacg ggcgcgcagg cggcgctgcc
      721 gatctggatc gacatcgcac ggcgggtcat cccttcacaa actccgacgt ttgtcatgcc
      781 gaccggagtg gtcacacgta ccatcgatcc gaaaaccggc cagctcgcta catcccaatg
      841 tccggagcag gtgtcggagg tcttcgtcga ggggacggca cccagcgtct attgtgaaat
      901 ccacggcggt gggatctggg aacgattccg gcggactttt ggattctcct aggtcttttt
      961 ttcgcggatg gtgtaagctg tcgtcaagtt gaatggacga gggagtgacg catgaagaaa
     1021 cccggcttta caggcgatga caacatcacg ctcttggcca agggcgtcga gctgaagggg
     1081 gaaattcgcg tcgaggggac ggtccgtatc gacgggtctc tcgaaggcga catccagacc
     1141 aagggccaag taatcgtcgg cgaagacgga ctcgtacagg gcacgatttc tgccggtacc
     1201 atcattagta gcggccgcat taaggcgacg gtcacagcca tcgagcggat tcaactgctc
     1261 aagaccgcca cccttatcgg agaggtgcat accccgattc tgatgatgga agagggggct
     1321 aagctccagg ggacgactga catgggtgtt gcggcctggc cggaggaact ccccaagttg
     1381 cccgggacgg tccgtaatct atcggcgcga cggacgaagc aggtcgtcgc gatcgatcac
     1441 gagccacgca cttagttgca caggagagga ttcttccctc cacccgttct gtagttctca
     1501 ttcacccgat gatctgagcc cgaacaaccg acggcgttct ctacttctcg tccagtggaa
     1561 tcgtaattgc gattccaccc atcacgtcat tgaccttgaa atctctcttc gggctgagcg
     1621 ggtgggcgtt atggcaggtc acgcaagcgg cggccacggc gcggtccggg taaatcgctt
     1681 gataatagcg tttttgtccg ctggtgacga tacctgcgac gggacgatcc gactggcgag
     1741 cgagcgtctc taaagccttc cgctcgaatt ccgtggctgg tgcgtttcgc tgatagatgg
     1801 gagagagccc gatgaggcgg aatcggatgc cgctgcctcc ttcggcgacg gccctacctg
     1861 attgttggag aaactgggcg ggaagcggaa gagcattgtc ttgctcccaa tgttccgctg
     1921 cagtgacgat gtgtttctcc tgcatccgat tgacgatttg gctggtatag atcgtccgat
     1981 cggcctgaat gacggcatgc acatatgccg ccactttttc ggcggggatg ctggctacgg
     2041 gagcctcccg cttcacatca ggcggcgtgg cggaggtcac tgatgctaag acgaggaaga
     2101 agatggatgc ggtgctgatg gaatgcccga cgcggtccat gatgcctcct gtggccccaa
     2161 ggatgactgg atcaccggca agctgatcgt gcagcatacg cccccacgat ccagattctc
     2221 aatgcggatg tcaccgccga atttgcggat gatccgtcga gcgaccgtca gtcctaatcc
     2281 tgagccttct ccttgtcctt tcatggtgaa aaacggatcg aacaccttgg agagatgctg
     2341 ggacggaata ccatgaccag aatcctcaat cgtgatggtc accattttat tgtcagcggt
     2401 ggtcgcgaca cggagcgtac cagggcccgg catcgcctgg accgcgttcg tcaggatgtt
     2461 cagaaacgcc tggcggagtt gctcaggact tgctgaaacc cggtctcgac atgcgtagct
     2521 cttgtggatt tcaaaacggc tgaggtcgtg tgtcgtctgc aacgccgcca gagcctgatc
     2581 gagctcgagg tggacgtcaa cgggaataag gcgcgttcct gtgtcacgtg cggccatgcc
     2641 ggtaaagtct cggatgattg cggccatgcg ccgtccgtgc tggacaatgt cacgggcatg
     2701 agccttgacg tggtcgaggt tggcctcctc ttgaatagct tctcctagtc ccaggatgcc
     2761 gaagagcggg ttgttcaact catggccgat gccggcggtg aggatgccca ggctcccaga
     2821 tttttcggct tggatgagtt ggtcttgtag gcgactctca tccgttgtat cgcgcaacac
     2881 gagaccaacg cgaggacctt cgcccgggcg cccgtcagtt cgaaaccatt cataatgata
     2941 gacccgctgt tcgatgcgca gttcgctccg tccgttcgtg cggccgcccg tgagtgtcgg
     3001 cgcaagaggg tcacggggtt cgtctgttga cgtctccatc gccctctcca tcgtcggcag
     3061 accagtggag tcccgttcct gaaattcctt tcgtaggcgt tgttgtgcgg gagcatcaag
     3121 cggcaggatg tcgaagaggc tcgtggaggg tgagagggac ggcgacaacc ccaacgactc
     3181 ctgcgcggcg tgattgacat attgcacctg ggcttgggcg tcaaccatga gaatgggggt
     3241 cgggactgca ttcaggatct ggtcggttga ccgctcgaga gactggactt cttgggtctt
     3301 cagggcgact tgatccgtga ggcctgcgaa cgccgcttcc agctgcttgt tcatccggtt
     3361 gaaatcttcg gccaacgctt ccagctcatc accggtttgg atctgaatcg actgacggag
     3421 ttcgccccgt ccgatcgact cggcggcctt ttgcagctgg cgaattggca acacgatccg
     3481 attggctgcc agatacccaa gggccacgag caggcaaacc gcgaccaagc caaagatggt
     3541 catccaggta aagagatggc gaatgggagc gaacaattcg tcggaggatt gccagacaaa
     3601 cgtgttccag gagccctcct caagcctacc gttcgtggcc cggctggttt cgggaagggg
     3661 ggcgaagccg atgatcgacg ttgagcgacc accatggccg tcactcggcg cggcaaccca
     3721 gccggggtga aagggagtta cgagggggat cagcgagaga tcagagatgc tcactccggt
     3781 gggaaggatc ggacaactca tgacaatccc ccggctgtcg atgagcatca catgtccggt
     3841 cttgccgaag cggatgggtt gaatggaagg agagaaaaac tctttggcat cgatcacgcg
     3901 atggagcact ccgaccactt cgtaatgaag gctgtccatg atggggagcg aaagggtgaa
     3961 cgcataggtt cccgcctgtt catgataact gacgtcctcg atatacagct gtccgattcc
     4021 cttgtgatag gcgccgatcc accacggtgc actgccgttt gagtacttgg ggcgtgtggt
     4081 cattgacgcg acgagcgatc cttggacatc tgtgacgaac aacattttcg tggccgcccg
     4141 gactacttgg ggcaggagct gttcctgctc actttgagac cccgtgtaat aactgcggag
     4201 gagattggac agggggccgc ctgtcacggt tttgacgata gcgggatcct tggcctccca
     4261 tcggatcgca tcctcattga tagttgcgcg aacagcgcgc agactctcct gatctctgcg
     4321 agtattgagg acgtcgcgcc ggtgttccag ttcggcgacg atcacgggat cccgtgcgat
     4381 gcgcgaggta cgggcgacct cctcggccac gaggatatcc agctttcggg ctgtctccgc
     4441 ggccagtgcc ttaaaactct cgccgttgac ttcttggatt tcgtgggatc cctgccagaa
     4501 cgccatcccc aatcccacga gcaagggaag gaccccgacc atcagcatag acagaatcag
     4561 cttcggttga agcccccagc ccttgcgggg tccggtccgg gatggatgtg atgaacccat
     4621 gacactcacc cgtgcgaaac ggtctcctgc gtgagaaagg tcagcgtgaa cgtcgaacct
     4681 ttatcgactt cgctgtcgac ggcaatgttg ccgtgcattt catggacgat gtttcgagca
     4741 ttatataagc cgagtcccgt ccctttccca gggggtttcg tggtgaagaa cggctcgaat
     4801 attttctccc tcagctctgg tgcgattccg cagccggtat cggagatgcg aacgtcgacc
     4861 gtatcggcca tcacgcgggt ttcgagagtc aacgttccgc ccacatgctc catggcgtgg
     4921 acggcattcg tgatcagatt cacgaacatg tgcaagagtt catccgggtt gccgcggacg
     4981 gctgctcctt gcgtatagtg cttgacgatc tcgatatcat gcagccgggc ggcgtaacgg
     5041 gctattttga gggcctcgtc gagcttcccg ttgaccgata caagacttgc ctctgaggca
     5101 gaagattgac gggaataccg cgtcaggtct tggcaaatgg ccgaggtccg tttgacggcg
     5161 tcgacaatct cgcgggcctg ttcccggatc gtcggaaggt cacgttcttc gatcagattt
     5221 tcggccaagc ccatgatgag ctgaagtgga ttgttgatgt catgtgcgat gccggtggcg
     5281 aacgatccga tcccggccaa cttttctgcc tgcattaatt cagcttgcaa cgcggattcg
     5341 tactggatga tctgccacag cagacgggat gcggaaccgc tgagcaactc tgtggttgcc
     5401 ggtttctgga tgcgcagaag cgattccatg cccggatcac ctgtgacatc caggatcacg
     5461 ctgatggcag ggtttcggat cagctcggcg acatcggtgg ttacgggaat ctcatactcg
     5521 cgggcacgct gcaaaccggg cgcacgcggg tcacggtcgg caatcccgat gacctcgatg
     5581 gacgaaatct gatggaacaa gtccaggagc gctctgcctc cgcgccctgc tcccacgatg
     5641 gcgactttcg tgggggggac gatgtccatg atgacggcct ttcgtgtgtg gtggtcacaa
     5701 ccgtgcgact gagggggaat gataagaggg gttcctcctc agccgcacag aggttggttg
     5761 tcaagcctcg ctagagcttt gccaactccc gttcctccat cgcgcgccgg actgcctctt
     5821 tcaatttttc tccctcgacc ggcttgacga gataatccac aacgccttgc cgcaagaacg
     5881 aggtcgccat gtcggtatcc gggaatccgg tcagcacgat caatggaacg cgaggataat
     5941 tgctgcggaa gtaggcaatc gcttcggctc cgttgatctt cggcatacgg atgtcacaga
     6001 tgaccacgtc gagcaggagg cggttctctc cttcattgat cgcctgaatg gctttttctc
     6061 cattttcggc ctcgatgacc tcatacccgg ccttttgtaa tgtcattctc accactttgc
     6121 ggatgtccgg ctcgtcgtcg acgacgagaa cccggccatt acaggcctca cctccagtga
     6181 agaatccaga tttgaactca gtcatgtcgt cctccttagc aatcggttga ggttgatgtg
     6241 tcactttgtt catgtccttc cgccaacttt tcgctgttgt ctctattatg cgtacagcaa
     6301 tgggcgtgcc agaatagttt gaggaaaacc ttctcgattt cctagcaatt tgaatatgcc
     6361 gtctctgcga ttaagcatgt tccggctgaa atgcaccacc atgtgctgag atccaccacc
     6421 atgaggtcct gttcgggaat cattgtg
//
LOCUS       DATDNW010000006         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_683992, whole genome shotgun sequence.
ACCESSION   DATDNW010000006 DATDNW010000000
VERSION     DATDNW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683992"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VI359_00145"
     CDS             <1..520
                     /locus_tag="VI359_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEY6972688.1"
                     /translation="CQAVRGYPVAILDTRKTLPGWRSLQKWAVALGGGINHRRSLSDG
                     ILIKDNHLVLLNQKEAVREASRLAKIAAPTGMPTIVEVESLKEVTQALAGQADIILLD
                     NMTPAMVKKALQLINKRALVEVSGGINLNNVRAMAAAGPDRISIGALTHSAPAATISL
                     ELYPSTKGRRRR"
     gene            526..1365
                     /locus_tag="VI359_00150"
     CDS             526..1365
                     /locus_tag="VI359_00150"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY6972689.1"
                     /translation="MQPSPPLSLDAIQRSLATASLGRRIELRDQIGSTNREAVSLAQA
                     GVEHGTVVLADSQSEGRGRLSRLWFSPPGVNLYCSIVIRKVIPADSLSEWLSWLPLTA
                     ASAAAEAIETVAATSVAVKWPNDLLINGRKVGGILCESGTNTRSGPFQVIGIGLNING
                     ERSDFPDELRETATTIRHEAGSFIDRNRLLAQLLYELEGCLDEYMQRGFERLAVAYGR
                     RCSTIGKTVKATLADGKELVGLAEAIGKDGSLTLVQRAMSDDERAPKVQQLRAADIVH
                     LRS"
     gene            1413..>2110
                     /locus_tag="VI359_00155"
     CDS             1413..>2110
                     /locus_tag="VI359_00155"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY6972690.1"
                     /translation="MLLVVDVGNTNVVLGIYDGPALKAHWRLATDAKTTSDEYGILFT
                     TLLSSAGLLPRQMTGAIVSSVVPALTGTFETLIEQYFGQRPLFVTAETDTGLTLRYAN
                     PKEIGSDRLVNAAAAYHRYRRDLIIVDFGTATTFCIVTKDAEYLGGVIAPGLGISAEA
                     LFSRAAKLSKVELTRPKTVIGTDTATSIQSGLLFGYAGLVDTLVKRIEQELGRSTYVI
                     ATGGLSTVIAPETTS"
BASE COUNT          474 a          601 c          600 g          435 t
ORIGIN      
        1 ttgccaggcc gtgcgtggct atccggttgc catcctcgac acgagaaaaa cgctgcccgg
       61 gtggaggtcc ctccagaaat gggcagtggc gctcggcggg ggaataaatc accgtcggtc
      121 gctgagcgac ggtatcctga tcaaagacaa tcatttggtg cttcttaatc agaaagaagc
      181 ggtacgtgaa gccagccgtc tcgcgaagat tgcggcaccg actgggatgc cgacgatcgt
      241 tgaagtggaa tcgctgaagg aagtcaccca ggccttggcc gggcaggcag atatcatctt
      301 gctcgacaac atgacccctg ccatggtcaa gaaagccctg cagctcatta acaaacgggc
      361 cttggtggaa gtttcaggtg gcattaactt gaacaatgtc cgcgccatgg ccgcagctgg
      421 accggaccgc atttccatcg gcgcactcac ccattctgct ccggcagcca cgataagctt
      481 ggaactctat ccatctacta aaggccgcag aaggcgttga tcagaatgca gccctcacca
      541 ccgctcagtc tagacgcgat ccaacgttct ctggccaccg cgtcgttggg acgccggatt
      601 gagctacgcg atcagatcgg ctcgacgaac cgcgaagcgg tctcgttggc tcaagccggt
      661 gtcgagcatg gcaccgtcgt gcttgccgat tcacagtcgg aaggacgagg ccgactttcg
      721 cgcctgtggt tttctcctcc gggcgtcaat ctgtattgct caatcgttat tcgaaaagtg
      781 attcccgccg atagcctctc cgaatggctc tcatggctgc cgctgaccgc tgcctcggct
      841 gcggctgaag caattgaaac ggtcgccgca accagcgtcg ccgtcaagtg gccgaatgat
      901 ctcctgatca acgggcgcaa agtcgggggc attctgtgcg aaagcggaac gaacacgcga
      961 tccggcccgt ttcaagtcat cggcattgga ttaaacatca atggagagcg gagtgatttt
     1021 cccgacgagt tgcgagagac ggcgaccacc atccgccacg aagcaggaag ctttatagat
     1081 aggaataggc ttctcgcaca gttgctctat gaacttgaag ggtgccttga cgaatatatg
     1141 caacgtggat tcgagcggct cgcggtggcc tacggacggc gctgttcgac gatcggaaaa
     1201 accgtcaagg ccacgttggc cgacggcaag gagttagtcg gactggccga ggcgattggg
     1261 aaggacggtt ctctgaccct cgtccaacga gccatgtcag atgatgagcg ggcaccgaaa
     1321 gtccagcagc ttcgtgcggc cgacatcgtt catctcagat cataatcagc agcctgctag
     1381 cgaaatcagg tcaaaccccg taaaatagat ttatgctgct tgtcgttgat gtcggcaaca
     1441 cgaacgtcgt cttaggcatt tatgatggtc cggcgctgaa ggcacattgg cgcctggcca
     1501 ccgatgcgaa gactacttcc gacgaatacg ggattctgtt caccactctt ctcagttccg
     1561 cgggactcct tccccgacag atgaccggcg ccattgtctc cagcgtcgtg ccggcgctta
     1621 ccggaacgtt cgagacactc attgagcaat acttcggtca acggcctctc tttgtgaccg
     1681 ctgagacaga cacgggcctc acactccgct acgcgaatcc gaaggaaata ggcagcgacc
     1741 ggcttgtcaa cgcggctgcg gcttatcaca ggtatcgtcg cgacctgatt atcgtcgact
     1801 tcggcacggc aacgactttt tgtatcgtga cgaaagacgc ggaatacttg gggggagtca
     1861 tcgcgccagg cctaggcatc tcagccgaag ctctcttttc cagagctgcc aagctatcga
     1921 aagtggaatt gaccagaccc aagacggtga tcggcaccga tacggccaca agcattcaat
     1981 caggcctgct cttcggatat gccggactcg tggatacgtt ggtgaagcgg atcgaacagg
     2041 aactcggccg ctcgacctat gtgatcgcaa ccggtgggtt gagtactgtg attgccccgg
     2101 aaaccacctc
//
LOCUS       DATDNW010000007         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_274399, whole genome shotgun sequence.
ACCESSION   DATDNW010000007 DATDNW010000000
VERSION     DATDNW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274399"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1236)
                     /locus_tag="VI359_00160"
     CDS             complement(268..1236)
                     /locus_tag="VI359_00160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY6972691.1"
                     /translation="MRDYLEFEKPIREIEEKIEKLAAATSGKPAPQDEIRKLRTKLAQ
                     IEHELYGHLTAWQRTQLARHPQRPSTLDYIGGLFRDFLEFHGDRSFGDDRAIVGGFAR
                     FNDRSVMVIGHQKGRTLKERMQRNFGMPNPEGYRKALRLMRMAEKFGRPIITFIDTPG
                     AYPGIGAEERGQAEAIARNLLVMSRLAVPIISVVIGEGGSGGALALAVSDRVLMLENS
                     VYSVISPEGCAAILWDDPAKVPDAAAALKMTAHDLKDLRIADDLIPEPLGGAHRDPKA
                     VTDRVAKALTNHLFTLTELSTDHLLAQREQKYRRIGAINGIEASPA"
     gene            complement(1302..>4467)
                     /gene="dnaE"
                     /locus_tag="VI359_00165"
     CDS             complement(1302..>4467)
                     /gene="dnaE"
                     /locus_tag="VI359_00165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY6972692.1"
                     /translation="GYQNLIKLVSKAYLEGFYYKPRMDKDILKEHHEGIIALSGCLSG
                     EIPYLIGQKDMEGAIAVAGEFQEIFGKDHFYLEVQANGLDHQRIANAGLIELHKKLGI
                     PLAGTNDCHYLKKEDSRPHDLMLCLQTGKTISDPNRLKFDTDQLYVKSTEEIAEAFAE
                     FPGAVSNTCTIADHCDLTLALNKTYLPQYRIPEGFKDRETYLEHLAIEGLNARLKERP
                     STIPATAYELRLREELMVICSMGFAGYFLIVWDIIRFARSRGIPVGPGRGSAAGSLVA
                     YALRITDLDPLVYTLLFERFLNPERVSLPDIDMDFCMDRRGEVINYVVDKYGSDHVAQ
                     IITFGTLGAKAAIRDVGRVLEMPYAEADKVAKLVPTQLNITLQQALDTEPRLRDLVET
                     DPKVKELMSVAQSLEGLARHASTHAAGIVISDEPLTNHVPLYKGTNDEIVTQYSMGDV
                     EKIGLVKFDFLGLKTLTMIRRAEMLINDSRPSEPPLAVDRLPFDDQKTFALLASGKTT
                     GLFQLESSGMRDLLTGLKPDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKIPIAYETP
                     ELEPILKDTYGVIVYQEQVMAIANKVAGFSLGQADILRRAMGKKKPDEMEKLRVKFLD
                     GAKKNKIAERKAEKLYELIQKFAGYGFNKSHAAAYAVVCYHTAYLKAHYPTEFMASLM
                     TTDMGNTDKIMGYFTECRELGINVLGPDVNESQKNFAVVDNAIRFGLAAIKNVGEGAV
                     ESVIAVRNEGGPFPSFFDFCRRVDLRKVNKRMLEGLIKAGAFDSTGAKRSQLMAVLDH
                     AVEDGAAAQRERDLGQTSIFAAAWSGHETEPHAALPPLPDIPEWDQGQRLKYERELTG
                     FYITAHPLTRYEATIRALSTATTGGLSEMSDGREVKLCGIITTVKTMLTKKGDRMAYL
                     TLEDLQGTVEVIAFPDLYKAAGALIAPERIVRLIGTIDRGDKGTKIRGSKIEPLADVQ
                     TQTVKRVLIRLSDRPEAKDQLPRLRDVLLRHPGATSVALTVLMDHTMEADTTPLPNVT
                     VTANEHFVADVEEVLGKGALSLLS"
BASE COUNT          925 a         1244 c         1263 g         1035 t
ORIGIN      
        1 cgacggacac gtcacttgcg atcgcagcgc cacccaggga gagattgtcg atgcggcctt
       61 ccccgcgtac cgagtcaccg gagaacgtca cgtttccgct gatagaaaat ctctcgaatt
      121 tccgttgctc catatctgac cgtcagcgta gcaagatctg cacaatcagc aagcatttta
      181 tgcgtgtcgg ccagatgacg tgtggttagt agatcgccac cctctcggcc tgaacaggga
      241 gactcagtgt aatcgggagc gacaatgcta cgcgggggag gcctcgatac cgttgatggc
      301 accgatgcga cggtacttct gttcgcgctg agccagcagg tgatccgttg agagttctgt
      361 gagagtgaag agatgattcg tcagggcctt agccacgcga tcagtaacag ctttaggatc
      421 gcgatgggct ccgccgaggg gttcaggaat aagatcatcg gcgatacgaa gatctttgag
      481 gtcgtgagca gtcatcttca gtgcggcggc agcatcgggc actttcgccg gatcatccca
      541 cagaatggcc gcacagccct ccggggaaat cacggaatac acggaatttt ccagcatcaa
      601 gacccggtcc gatacggcta aggccagtgc accgccgctc ccgccttctc cgatcaccac
      661 ggagataatc gggaccgcca accgcgacat gactaacaga ttccgtgcaa tggcttcggc
      721 ctggcctcgc tcttcggctc cgattccagg gtaggcgccg ggggtatcaa taaacgtaat
      781 aatcgggcgt ccgaacttct cggccatacg catcaaccgc agcgcttttc tgtagccttc
      841 cggattcggc attccaaagt ttcgctgcat ccgctccttc agcgtcctgc ctttctgatg
      901 gccgataacc atgaccgatc gatcgttgaa ccgcgcaaaa cccccgacaa tggcccggtc
      961 atcgccaaat gaccgatcac catggaactc gaggaagtct cgaaagaggc cgccgatata
     1021 gtccaaggtg ctcggccgct gggggtggcg tgcgagctgc gtccgttgcc aggcggtaag
     1081 atggccgtac agttcatgct caatctgcgc gagtttcgtg cgcagcttgc ggatttcatc
     1141 ctggggggcc ggcttgcctg aggtcgccgc cgcgagtttt tcgatctttt cttcgatctc
     1201 gcggatcggt ttctcgaatt cgaggtaatc gcgcatagac atcaatggga catgtgccgt
     1261 taggggaggg gctacgggca ccgcaccccg aacatactct gttacgatag caaagacagg
     1321 gcccctttgc ctagcacttc ttcaacatca gccacgaagt gttcgttggc cgtcacggtg
     1381 acgtttggga gcggtgtcgt gtcggcttcc atggtatggt ccatgaggac cgtcaaagcg
     1441 accgaggtgg cgccgggatg ccggagcagg acgtcgcgca atcgtggcaa ttgatctttt
     1501 gcctccggtc ggtcactgag acgaatcagg acgcgtttga cggtctgagt ctgcacatcg
     1561 gccaggggtt cgatcttgct gccacggatt ttggtacctt tgtctccacg gtcgatcgtg
     1621 ccgatcaatc tgacaatccg ctccggtgca atcaatgctc cggccgcctt atagaggtcg
     1681 ggaaaggcga tcacttcgac cgttccttgc agatcttcaa gggtcaaata cgccatccgg
     1741 tcgcccttct tggtaagcat cgtcttgacc gttgtaatga tgccgcagag tttcacttct
     1801 ctgccgtcgg acatctccga caatccgccg gttgttgcag tcgaaagcgc tctgatcgtc
     1861 gcttcatatc gggtaagtgg atgggccgtg atatagaagc cggtcagttc acgttcgtat
     1921 ttgaggcgtt gaccttgatc ccattctgga atgtccggga gaggcgggag agccgcatga
     1981 ggttccgtct catggcccga ccaggctgct gcgaaaatac ttgtttgtcc gaggtctcgc
     2041 tcccgttgcg cagccgcgcc gtcttcgacc gcgtggtcga gcaccgccat gagttgcgat
     2101 cgtttggcgc cggtggaatc aaaggcgccc gctttgatca acccttccag catccgcttg
     2161 ttgaccttgc gaagatcgac ccggcggcag aaatcgaaga aggacgggaa gggccctccc
     2221 tcgtttcgaa cggcgatgac ggactcgacc gcaccttcac ccacgttctt aatggctgcc
     2281 agaccgaatc ggattgcgtt gtcaacgacg gcgaaattct tctggctttc gttgacgtcg
     2341 ggccctagca cgttgatacc aagttctcga cattccgtga aatagcccat gatcttgtcg
     2401 gtgttaccca tgtcggtcgt catgagtgac gccataaatt ccgtcggata atgagccttg
     2461 agataggcgg tgtgataaca gacgactgca taggccgcgg catgggattt gttgaagcca
     2521 tatccggcga acttctggat gagttcgtag agcttctcgg cctttctctc ggcaatcttg
     2581 ttcttctttg ccccatccag aaatttgacg cgcagctttt ccatctcgtc cggcttcttt
     2641 tttcccatcg cgcgccgaag aatgtcggct tgtcccagcg agaagccggc aaccttgttc
     2701 gcaatcgcca tgacctgttc ctgatagacg atgacaccat aggtgtcctt caggatcggc
     2761 tcgagctccg gcgtctcgta ggcgatagga atcttgccct gtttgcgctt gatgaagtcc
     2821 ggaatcagat ccatcggacc tggccgatat agggcgatga tcgctatgat gtcctcgaac
     2881 cgatccggtt tcagaccggt gaggagatcc cgcatacccg agctctcgag ctggaacaac
     2941 ccggttgtct tgcctgaggc gaggagggcg aacgttttct ggtcgtcgaa gggtagtcgg
     3001 tctactgcca gcggcggttc gctcggtcgg ctgtcgttga tgagcatttc cgcgcgtcgg
     3061 atcatcgtga gtgtcttaag cccaaggaag tcgaacttca ccaggccgat cttttccacg
     3121 tcacccatgg agtactgcgt cacgatttca tcgttcgtcc ctttgtagag cgggacgtga
     3181 ttggtcaacg gctcatcgga gatgacgatg ccggccgcgt gagtcgaggc atgacgagcc
     3241 agaccttcca acgattgtgc gacggacatc agctctttga ccttgggatc ggtttcgacc
     3301 agatcacgga gtcgaggttc agtatcgagg gcttgttgca aggtgatgtt caattgggtc
     3361 ggtaccaact tggcgacttt gtcagcctcc gcatacggca tttccaacac acggcccaca
     3421 tcgcggatcg cggccttcgc gcccaaggtg ccgaacgtaa tgatctgtgc cacatggtcc
     3481 gagccatact tgtcgacgac atagttgatc acttccccgc gtcgatccat gcagaagtcc
     3541 atgtcgatat ccgggagcga cacgcgctcg ggattcagaa accgctcgaa gagcagggta
     3601 tagacgagcg ggtccaaatc ggtgatgcgc aacgcatacg ccaccaaact gcctgcggca
     3661 gagccgcggc cgggaccaac gggaatgcca cgcgagcggg cgaaccggat gatatcccac
     3721 acgatgagga agtaaccggc gaatcccatc gaacagatga ccataagctc ttcgcgcaac
     3781 cggagctcgt acgccgttgc ggggatcgtg ctgggacgct cctttagccg cgcattcaat
     3841 ccttcaatcg cgagatgctc aagataggtc tcgcggtcct taaacccttc gggaatccta
     3901 tactgaggaa ggtacgtctt gttcagcgcc agcgtgagat cgcaatggtc ggcgattgtg
     3961 caagtgttgg aaactgcgcc gggaaattca gcaaatgcct cggcgatctc ctccgtcgat
     4021 ttcacgtaca gctgatcagt atcaaacttc agccgattcg ggtcgctgat ggtcttaccc
     4081 gtttgaaggc agagcatcag gtcgtgcggg cgggagtctt cctttttgag gtaatggcaa
     4141 tcattcgtgc cggctaaggg aatgccaagt ttcttgtgaa gttcgatcaa cccggcgttg
     4201 gcaatacgct gatgatcgag accgttggct tggacctcaa gatagaaatg gtcttttccg
     4261 aaaatctcct gaaactcacc cgcgacggct atggccccct ccatatcttt ctggccgatt
     4321 aggtaaggga tttcaccgct caaacaaccg gaaagagcga tgattccttc gtgatgctcc
     4381 ttgagaatat ctttatccat ccgaggctta tagtagaatc cctcaagata ggctttactg
     4441 acgagtttaa tgaggttctg atagccg
//
LOCUS       DATDNW010000008         4663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1230721, whole genome shotgun sequence.
ACCESSION   DATDNW010000008 DATDNW010000000
VERSION     DATDNW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230721"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VI359_00170"
     CDS             <1..246
                     /locus_tag="VI359_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972693.1"
                     /translation="QGCVSLGGVCVMSWGYWGIDMGLVGLVAMLFVCLQLVYSNSKTS
                     PDASPSLRDRSGEQSQKQPVAVDWPHRELRQVGTIQS"
     gene            complement(457..633)
                     /locus_tag="VI359_00175"
     CDS             complement(457..633)
                     /locus_tag="VI359_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972694.1"
                     /translation="MSASVERGYQETLGYVYGHRGVVGGSVLSCWMVLEMLAAGVVFD
                     EERLGFLLPNGQVE"
     gene            988..1533
                     /locus_tag="VI359_00180"
     CDS             988..1533
                     /locus_tag="VI359_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEY6972695.1"
                     /translation="MENRGMTVILAVYMAALFGSAGATESPYGASQTGRFAILETFKQ
                     EAVLDLRTQLVWERSPHPAEVTWSTAHTRCALKTVGGQTGWRLPSFIELMTLVEPHVY
                     QAPAVPTLPPGHPFQGIKAGAYWTSDSSSSEPAQAYTVDLLRADVATQEKSQVHPLWC
                     VRGGIDEQPSQSSSVRPSGMI"
     gene            1671..2216
                     /locus_tag="VI359_00185"
     CDS             1671..2216
                     /locus_tag="VI359_00185"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6972696.1"
                     /translation="MRDRIRFKAGVLGAIVVMAIAIPLGYAAEFSRDAAAKYILATAK
                     AFRTVYTKQVVAQAAKSNVKPDENWTKDDHAIMLPAQFLKAAGSELKDFELGLIGLTP
                     IYKENLPRTKAEEDALKKLAANPATGILTFEDGNQFKGIAADLAIADSCVSCHNAHPA
                     SPKKDFKKGDLMGAIVVRFAK"
     gene            2641..2934
                     /locus_tag="VI359_00190"
     CDS             2641..2934
                     /locus_tag="VI359_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972697.1"
                     /translation="MHRQSFIHCAILSGAFTFFMGMLMSSAYAADPSPNTPVPSAIIA
                     EGNALASGSIEDSLKACLARIPNDATTGQRMIAEQGCRRDEADRKPIQAVPGR"
     gene            3066..3194
                     /locus_tag="VI359_00195"
     CDS             3066..3194
                     /locus_tag="VI359_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972698.1"
                     /translation="MVIVQNLLPWLTHHLMGAVVISYVTGILGVLVYTFLEPRDQR"
     gene            3244..3507
                     /locus_tag="VI359_00200"
     CDS             3244..3507
                     /locus_tag="VI359_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6972699.1"
                     /translation="MGKRVLYVGGLSDTISDYQLRDLFSTYGVVGRAYIVRHKHTGKS
                     AGYGFVEMGSGEQALQAVVALEGAQFAGNCLRLYVTPYVSHSS"
     gene            3964..4302
                     /locus_tag="VI359_00205"
     CDS             3964..4302
                     /locus_tag="VI359_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972700.1"
                     /translation="MSTPKQNNPHTTSPWIRIPDGTMVKHRHEGHEGFIDGLTELVTG
                     PARNPDGKTQYRMNVGAPTRQLVTEDDLCILIDAENLVRMMKQKEPYRRSVTEQLHGI
                     FADDRFIKSV"
BASE COUNT         1045 a         1372 c         1205 g         1041 t
ORIGIN      
        1 caaggctgtg tatcactcgg cggagtgtgc gtcatgtcat ggggatactg gggcatcgac
       61 atgggtctcg tggggttggt ggccatgctc ttcgtctgtc tgcagttggt gtactccaac
      121 tccaagacat ctcccgacgc atcgccttcc ctcagggatc gatcaggtga gcagtcgcaa
      181 aaacagccgg tggcagtcga ctggccgcat agagagctca gacaagtcgg cacgatacag
      241 agctgatgtc ttattaggtg ggccatacgg tcgagatcgt tggtctcgtg atgattggcg
      301 cgctcattga acaaggtgca gcttctcaaa accgccgtcc agatcgggga tgtgcaatca
      361 ccgattctct cgattgaaga gggagcgtac ttcaaggggt tcgcagacat gggggccagc
      421 ccatgcaccg acgagtcccc tcagagcgac gatcctttac tcgacttgac cattcggcaa
      481 aaggaatccg agccgctcct cgtcgaacac gactccggca gctagcatct ccaacaccat
      541 ccaacacgag agtacacttc cgccgaccac gccgcggtgg ccgtatacgt aaccaagggt
      601 ttcctgatac ccccgctcaa cggatgcgct caccctcatc ttcccggcaa gtgccccaac
      661 gctgtaactg gcatatccgt cccgcccgga gtcgcattct cgatgaggag tttttcagct
      721 ttgctgccga aaattggcct tgtgcagtgc aggaaagaac gctcgccccg ctctcaagaa
      781 ctatgattct ggatccaccc tcccggttcc tttcacacag agctgcaaaa ctcttcaaaa
      841 acaatagacc tgcctgactt tacctctctc aaattcgcat ccccgctcct tctgacctcc
      901 gcttgcacgg cttggatccg gaggtccagg ttttgctgac gtcatccacc cgtcagtcgc
      961 caccacctct gttgagaagg atcgcgaatg gagaatcgag ggatgacagt aatcctcgcc
     1021 gtctacatgg cggctctatt cggaagtgcg ggcgcgactg agtctcctta cggagcctcc
     1081 caaaccgggc gatttgcgat cttggagacc ttcaaacaag aagctgtctt ggacctcaga
     1141 acccagttgg tttgggaacg atctccccat cccgctgaag tgacctggtc gaccgcacat
     1201 acccgctgcg ccctgaagac tgttggagga cagactgggt ggcgtcttcc ttcgttcatt
     1261 gagctcatga cactcgttga accacacgtg taccaggcac ctgcggttcc gactttgcct
     1321 ccgggacatc ccttccaagg gatcaaggcg ggggcttatt ggaccagcga ttcctcgtca
     1381 tccgaaccgg cacaggcata tactgtcgat cttctccgtg ccgacgtggc gacgcaagag
     1441 aagagtcaag tgcatcccct ctggtgtgtg cggggtggga tcgatgagca gccctcgcaa
     1501 tcatcctcag tacggccttc aggaatgatc taaccgtttc agtccgcatc tttcacgtgt
     1561 tgaacaattt cacaatccgc gaataagcta cttgacaact ccatccacgg ggcgcaccgt
     1621 attcgtctat tgcgccgttc tgaccgacaa tctcaacctg gaggtggggt atgagggaca
     1681 ggatcagatt caaagcaggt gtgcttggag cgattgttgt gatggcaatc gctattcccc
     1741 tggggtacgc ggcagaattt tctcgcgatg cggcagccaa atacatactc gcaacggcca
     1801 aggcattccg cacggtctac accaagcagg tcgttgccca agccgctaaa tcaaatgtga
     1861 aacccgacga aaactggacg aaagacgacc acgccatcat gctaccggcc caatttctga
     1921 aggccgcagg aagcgaactc aaggacttcg aactcggcct gatcggtttg acccctatct
     1981 ataaggagaa cttgcccagg accaaggcag aggaagacgc gctcaaaaag cttgcggcaa
     2041 atccggctac cgggattctg acgtttgaag acggcaatca gtttaagggc atcgccgccg
     2101 acttggccat tgccgactcc tgcgtctcct gccacaatgc ccatccagcc agcccgaaga
     2161 aggatttcaa gaaaggcgat ctcatgggcg caatcgttgt acgatttgcg aagtaattcc
     2221 ttttcacgat tcgatgcggg cacaaaggcc ggacgagagt cccctctcct ccggcctggt
     2281 tattcacgcc tgctgctgtc taaaccgaat tgaaaaacct cctgccgcgc cgtgaaatcg
     2341 ttctcgacag catgttcccg ctgctttata ccctcctggc aaatcaaatc agcgctcgct
     2401 atctgggcca atgtgtgtta gcgtaattca tagttttgaa ctacggtcac tacctgatcc
     2461 ctcgcgcctc cctcccacct tgttagctgc tgcgcgatcg atgctctagt ttccgccgtt
     2521 cctccgaata gccggctcct tacgagcccc gactcaggac ctcaggccct gatcactcga
     2581 tcgtcacggg ccacgacgag tgtctgctgt cctcgccctc accacggaaa ggacacaagt
     2641 atgcatcgcc aatcattcat ccattgcgcc attctctctg gggccttcac cttcttcatg
     2701 gggatgctta tgtcatccgc atacgcggcc gacccctctc cgaacacacc tgttccatcg
     2761 gcaatcatag ctgaaggcaa tgcgctcgcc agcggttcaa tcgaagattc tttgaaagcc
     2821 tgtctggcaa gaattcccaa cgatgcgacg accggacaac gcatgatcgc cgaacagggc
     2881 tgccggcgag acgaagccga ccgcaaacca attcaagctg ttccgggccg gtagggcatt
     2941 ggcgcttccg caatggttta cgtgaatgtg cggcctgaca cgattgtccc cgtgcgggat
     3001 gaccactgaa cgtcgtggaa ccgtgctgag agtaaccgat ctctgataaa ggggccgcga
     3061 gtctcatggt gatcgtccag aatctgctcc catggctcac ccaccatctc atgggtgcgg
     3121 tcgtgatttc gtatgtgact ggtatcctcg gggttctcgt ctataccttc ttggagccta
     3181 gggaccagcg ttaacgcagt ctgcccccta gtcgtccaat gtgagagcag aggaggaacg
     3241 ttcatgggaa agcgagtttt gtatgtcggc ggcctctcgg acaccatttc cgattaccaa
     3301 ctccgagatc tcttcagtac ctacggagtg gtcggtcgag cctatatcgt tcgtcataaa
     3361 cataccggga aatcggccgg gtatggtttc gtcgaaatgg gctctggcga acaggcatta
     3421 caggccgtgg tggcgcttga aggcgcccaa ttcgccggta actgtctccg gctctacgtc
     3481 acgccctatg tctcgcacag ttcctaatta agaagcagct atgtaagggg agaaccgatg
     3541 agccgtatca acctggacct tctcatgact ttgccggatg gttcgcacct tgtgatcagc
     3601 acccagtgtt cgagagaggg agatttctct tgtgcactct actccgcgac catcgcggaa
     3661 gatgattgcg ccgcctttca gatgatctca aaccatctct ctgcgtcaac gtgtttgggc
     3721 gcgcaggaac atgcgtacgg ctatgcagtc cggatgtacc ctcgtgcggc tgagttcatc
     3781 aagaaaccgc cgtacctgat ctggtctgga ccacaatccc atagccaagc ctaacaaccg
     3841 gccgagtggc ggagaatgat cttgggcatc atcaggctcc cctcgagcct tcatagatcg
     3901 caacgagtgg acgatatatg gcgaagaacg gcatactcac caatacaggg agacacaggc
     3961 actatgagca caccgaagca gaataacccc cataccacgt caccgtggat acgcattccg
     4021 gatggaacca tggtcaaaca ccgtcatgaa ggccacgagg gattcatcga tggactcacc
     4081 gagcttgtca cagggcctgc ccggaatccc gacggcaaga cacaatatcg tatgaacgtc
     4141 ggggcgccga ccagacaact tgtcacggag gacgacctct gcattctgat cgatgccgag
     4201 aacctcgtga ggatgatgaa gcagaaagag ccttatcgtc gctccgtcac agagcagttg
     4261 cacggtatct ttgccgacga ccgatttatt aaatcggtat aagaggtaac cgtgtgcgtc
     4321 gctccgatcc atcatagggt cttggtgaca gcagattcgc tcacacggtc ggcttgagta
     4381 gctattttgt tcctgttctg gtataaatac cgtgtttcat tgcgacgggc ctcgcgtgaa
     4441 gcatcatatc gaacggccca tcaccgatgt tgttgtcacc gagaatttga tcggaagtct
     4501 gacctaagcc gcctcactga tcgcgtcttc ggcccgctcc ttcccctctc ctgctcgcgt
     4561 ctcagacaat gtcattatca gaaggaggaa cccccatggg ttcaaaacta tatgtcggcg
     4621 ggttgccgta tgcggcgacc gaatcacaac ttaccacctt gtt
//
LOCUS       DATDNW010000009         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_411547, whole genome shotgun sequence.
ACCESSION   DATDNW010000009 DATDNW010000000
VERSION     DATDNW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411547"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VI359_00210"
     CDS             complement(<1..738)
                     /locus_tag="VI359_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972701.1"
                     /translation="MNLFEKATQVLEWPRLLEALARHAQSTIGMERCRVLTLSADIIG
                     AQRHQQETSEMRRLQDSSDPLPTLSFPDIRELLVRAQKGATLEAHELRDCAVALKLSR
                     AVDSVLGRHRLEAPKLADVVAPLRTDVELVRLRNALEAAIDDDGSIKESATPELKRLS
                     HHSQVLKQQMRHHLEQILHSRRYAEVLQEQYFAQREGRYVVPIKTDMRGRIPGIVHDI
                     SASGATVFLEPRELVELNNSIKVADLEV"
     gene            993..2006
                     /gene="gap"
                     /locus_tag="VI359_00215"
     CDS             993..2006
                     /gene="gap"
                     /locus_tag="VI359_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY6972702.1"
                     /translation="MAIRIGINGFGRIGRNVLRASLGDPDLQFVAINDLTDAKTLAYL
                     LKYDSVHGTLKGTVEAKDDQILVDGKAIKVLAIKDPKELPWKDLNIDVVIESTGRFTD
                     REGAGKHLSAGAKSVIISAPAKDPDVTIVLGVNENTYDPKSHHIVSNASCTTNCLAPV
                     AKVLMETFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKMDGLAIRVPTPNVSLVDLTVETEKDCDVASVNEAFRKAANGPMKGILEYS
                     EAPIVSIDQKGDDHSATVDAPLTTVIDKRLVKVTAWYDNEWGYSCRVRDLIKVIAAKS
                     KGR"
     gene            2090..3289
                     /locus_tag="VI359_00220"
     CDS             2090..3289
                     /locus_tag="VI359_00220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY6972703.1"
                     /translation="MNLHKLTIDDVQLRNQRVIIRADFNVPLDDSLQITDDTRIRSTL
                     PTINHVVDDGGKVILCSHLGRPKGKFDPKYSLAPVAKRLGRLLGKEVIFAPDCIGHAV
                     EALVAKMKPGDVMLLENLRFHPGEESNDDAFSKALAALGDVYINDAFGAAHRAHASTV
                     GITKFIKTSVAGFLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKRVDK
                     VIIGGGMAFTFLKAKGLEIGNSLVENDMLDFAKGIEEHAFSRGVKFYLPVDCVVAASR
                     ELSAESKIVPVQEIPKDWYALDIGPASVKLFTEAVQNAKTILWNGPMGVFEIDAYARG
                     TLSIAHAIADAYALTIVGGGETAMAVHRAGESENISFISTGGGAALELLEGKPLPGLT
                     ALPERQD"
     gene            3333..4103
                     /gene="tpiA"
                     /locus_tag="VI359_00225"
     CDS             3333..4103
                     /gene="tpiA"
                     /locus_tag="VI359_00225"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY6972704.1"
                     /translation="MRTPFIIGNWKMNKTSSEASAFVPQLLGRLPRPLNCELVLAPPF
                     TALESVRIALGPSSEIGLAAQNLHWESHGPFTGEVAGPMLRDLGCRYVIVGHSERRSL
                     FGERDDEVQKKIRAALTHDLHPILCVGESLTERETGQTESVVTTQLKRALADRSEQEL
                     SRIAIAYEPIWAIGTGRAATVEQAAAVHRSIRGFIATGWNKEVASTMRVLYGGSVTPQ
                     NIGSLLATDDIDGVLVGGACLDPDSFARIALAGHKKHS"
     gene            4120..>4284
                     /gene="secG"
                     /locus_tag="VI359_00230"
     CDS             4120..>4284
                     /gene="secG"
                     /locus_tag="VI359_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY6972705.1"
                     /translation="MYTLLVIIHVFVCFLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFT"
BASE COUNT          954 a         1232 c         1183 g          915 t
ORIGIN      
        1 aacttcgaga tccgcgactt tgatggagtt gttcagctca accaactcac gcggttcgag
       61 aaacaccgtg gcgccgctgg ctgatatgtc atgcacgatg ccggggatgc gtccgcgcat
      121 atccgtctta atcggcacaa catatcgccc ttcacgctga gcaaagtact gttcctgcaa
      181 gacttcggcg tagcgacgtg aatggagaat ctgctcaagg tgatgtcgca tttgctgctt
      241 gagaacctga ctatgatgag acaatcgctt cagctccggc gtggcggatt ctttgattga
      301 tccgtcgtcg tcaatcgcag cttcgagcgc gttcctcaaa cggacaagtt ccacgtcagt
      361 ccgaagcggc gccaccacat ccgccagctt gggagcttcc agtcgatgtc gtcccaacac
      421 gctgtccact gccctgctca atttcagcgc gaccgcacaa tcgcgtagtt catgagcctc
      481 gagtgtggct cccttctgtg cccgcaccag cagctcccgg atatctggga acgagagggt
      541 gggaagcgga tcgctgctgt cctgcagccg tctcatctcc gatgtttctt gctggtgccg
      601 ttgcgcacca atgatgtcgg cgctcagggt aaggacacgg caacgttcca tgcctatcgt
      661 cgactgcgca tgacgggcca gagcctcaag gagtctgggc cattccaaca cctgcgtggc
      721 tttctcaaat agattcacga ctccctcatc aaattaagat tttgcgtgag ggtagactct
      781 agcgaaacga gcgtcatgga cgcaaggcaa acgccgatca ggtagagacc aaccatggta
      841 catccctttt cgagtcagat agaaactcct caagtccgac gcggggttac cccttccgta
      901 tagcttgaca tggtgcggag ccaccgatac tatcgccgtc ggtctcatgc gcgaaatcac
      961 cgtgtacttt ctattctgag gaactgaaca ctatggctat caggatcggg atcaatggct
     1021 ttgggcgcat cgggcgaaat gtgttgcggg cctcgttggg cgaccctgac cttcaatttg
     1081 tggcgatcaa tgacctcacc gacgcgaaga cgctcgccta cttgttaaaa tacgactcgg
     1141 ttcacgggac gctgaaagga accgtcgagg ccaaggacga tcagattctt gtcgacggga
     1201 aagccattaa agtcctggcc atcaaggatc cgaaagaact cccttggaaa gatctcaata
     1261 tcgacgttgt gatcgaatcg accggacgat tcaccgaccg ggagggcgcg gggaaacatt
     1321 tgtctgccgg cgcgaagtcc gtcatcatat ccgcgccggc gaaagacccg gatgttacga
     1381 tcgtgctggg cgtcaacgag aacacctacg atccgaaatc tcaccacatc gtctcgaacg
     1441 cgtcctgcac gacgaactgc ctggctcccg tggccaaagt cttgatggag accttcggca
     1501 tcaagcacgg cgtgatgacg acgatccatt cctatacgaa cgatcagcag ctactcgatt
     1561 tgccccataa ggacttgcgt cgcgctcgag cggccgggat gtcgatgatc cccaccagta
     1621 cgggagccgc aaaggccctc caccttgtca tccctcaact caaaggaaag atggacggcc
     1681 tcgccatccg tgtcccaacc ccgaacgtct cgctcgtgga cctgacggtg gaaaccgaaa
     1741 aggattgcga cgtcgcttcg gtcaatgaag cgtttcgtaa ggcagccaac ggcccgatga
     1801 aaggcattct cgagtactcc gaagctccca ttgtctcgat tgaccagaag ggtgacgacc
     1861 attccgctac cgtagatgca cccctcacca ctgtgatcga caagcggctg gtgaaagtga
     1921 ccgcctggta cgacaatgaa tggggctatt cgtgccgagt gcgggatttg atcaaagtta
     1981 tcgccgccaa atcaaaaggg cgctgagttg ttccgaacac tccggtttca ccccagtaac
     2041 ctgcgacggt tgatgcgttg atccgacagg gccggtacag ggagcgtgca tgaatcttca
     2101 taagctcaca atcgacgatg tacagctacg taaccagcgg gtgatcatcc gtgccgattt
     2161 caacgttcca ttagacgact ccctgcagat tacggacgac acgcgtattc gctcaaccct
     2221 cccgaccatc aatcatgtcg tcgatgacgg agggaaagtc atcctctgct ctcacctcgg
     2281 ccgacccaaa ggcaaattcg atccgaagta cagcttggct ccagtcgcaa aacgcttggg
     2341 acggctgctt ggcaaggagg ttatcttcgc cccggattgt attggccatg ccgtcgaagc
     2401 actcgtggcc aaaatgaaac cgggcgatgt gatgttgctc gaaaatctcc gcttccatcc
     2461 aggggaagag agcaacgacg acgccttttc gaaagctcta gcggctctgg gcgacgtgta
     2521 tatcaacgat gccttcggtg cagctcatcg agcccacgcc tcgacggtcg ggattaccaa
     2581 attcatcaag acctcggtcg cgggctttct cctcaagaaa gagatcgaat acctcgaggg
     2641 ggccgtcgaa aaccccgtgc gacccttcgt cgccattctc ggcggcgcga aagtgtccgg
     2701 aaagatcggc gtcattgaga atctcggcaa gcgcgtggat aaagtcatca tcggcggggg
     2761 catggcgttt acctttctga aggccaaggg actcgagatc ggcaactcat tggttgaaaa
     2821 cgacatgctg gattttgcca aaggcatcga ggagcatgct ttttctcgtg gtgtgaagtt
     2881 ctacctgccg gtggactgcg tcgtagcggc cagccgcgaa ctcagcgcgg aatcgaagat
     2941 cgtccctgtg caggagatcc ccaaggattg gtatgcgctc gacatcggcc ccgcctccgt
     3001 gaagcttttc accgaagccg tgcagaacgc caagacgatc ctctggaacg gcccaatggg
     3061 ggtgtttgaa atcgatgctt atgcacgcgg cacgctctcc atcgctcacg cgatcgccga
     3121 tgcctacgcc ctgacgatcg tcggtggcgg cgaaactgcg atggccgtcc atcgagccgg
     3181 agaatcagaa aacatttcgt tcatctcaac cggtggcggc gcggcgctgg aactcctgga
     3241 aggcaagcca ctgccggggt tgaccgcgtt gccggaacgc caagactaac acctctggtc
     3301 tgataccgcc cacgccgata ctttgaaacg ctgtgcgcac accattcatc atcggaaact
     3361 ggaaaatgaa caagacctcg tcggaggcgt ctgcgttcgt ccctcaactc ctcggtcgat
     3421 taccgcgtcc cctgaactgt gagttggtcc ttgccccccc ctttacggcc ttggaatccg
     3481 ttcgtatcgc cctaggtccc tcctccgaga tcggtcttgc ggcccaaaat ctccattggg
     3541 aaagccatgg ccccttcacc ggtgaagtgg ccggtcccat gttacgcgat cttggatgtc
     3601 gctatgtgat cgtgggacat tcggaacgtc gctctctctt tggcgagcgc gacgacgagg
     3661 tacaaaagaa aataagggcc gcactcaccc atgatctgca tccgattctc tgcgtgggag
     3721 agtctcttac cgaacgggaa accggccaga cggaatcggt cgtgaccaca caattgaagc
     3781 gcgcactggc agaccgatct gagcaggaat tgtcacggat tgcgatcgct tatgagccga
     3841 tctgggccat tggcacaggg cgggcggcaa ccgtcgaaca agcggcggct gtccatcgct
     3901 cgattcgcgg ttttattgcg acgggctgga acaaggaagt ggcctcgacg atgagagtgc
     3961 tatacggagg gagtgtgacg ccacaaaaca ttggctccct gttggcaact gacgacatcg
     4021 atggcgtact tgtcggtggg gcttgtctcg atccggactc gtttgctaga atcgccctcg
     4081 ctgggcacaa gaagcattct taatcgatcg gaacagagta tgtatacctt gctcgtcatc
     4141 atccacgtgt ttgtttgttt cctcatgatt ggggcaattt tgctgcaatc gggcaaaggg
     4201 gccgaaatcg gcgcggcttt cggcggatcg agccaaacgg tattcggcag ccgtgggccg
     4261 gctaacttcc tgagtaaatt cacc
//
LOCUS       DATDNW010000010         9430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1162600, whole genome shotgun sequence.
ACCESSION   DATDNW010000010 DATDNW010000000
VERSION     DATDNW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162600"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VI359_00235"
     CDS             complement(<1..716)
                     /locus_tag="VI359_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY6972706.1"
                     /translation="MLAGFGLVFAGIVVISIASYRNTSVLIKNSRLDTRSHELVQLLG
                     SIGEALDDAERGHRRYLVTGDEAYLKGHKTVLEQMPVFSQYLRGLTEGTSDQQERVAS
                     LERLIGQQLDAEAAAISARETNGYEGVRRIALSGAAKQELDAIHRLIVELETTEQKAL
                     RSRVIQSTGSTRNTIVLLVLGALLQLVLLASVYYLIHHDVTERRRVAAELRRRGELLQ
                     AANKELEAFSYSVSHDLRAPL"
     gene            complement(993..2486)
                     /locus_tag="VI359_00240"
     CDS             complement(993..2486)
                     /locus_tag="VI359_00240"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY6972707.1"
                     /translation="MNFSLAMSLSDLLLLLPEMLLTFWICLILVVDFAFPRLPKEQLA
                     YLSIGGLAITLGCLAWFDVTGITGTLFANMFVVDRMALFFKMLIVLATILVIIASIEY
                     VRRFTFFRGEYYFLVAMSALGMMFMASANDLLSVFVTLEFSTFGFYVLVAYLREDAAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMTSAQATPGLIIGYLLIFAALG
                     FKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKGAAFAILLRMFYVALASFKPTWVLML
                     VAASILSMTYGNIVAIAQKNIKRLLAYSGIAQIGNVLIGLAAGTKMGSDAILFYLLTY
                     LFANLGAFAIVIAVSQAIGSDEIEDYSGLGRRSPFLAFAMLIFLLSLAGVPPLAGFIG
                     KIYIFVAAIKEGLYTLITVGLINIVISMYYYLIVVKKMYIIEPHDPSPVSISGPMKAV
                     VYVGLAGTLAIGIYPQPVIDWVVAATLMFSNIAGPAASIPPPSPFGG"
     gene            complement(2483..4174)
                     /locus_tag="VI359_00245"
     CDS             complement(2483..4174)
                     /locus_tag="VI359_00245"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6972708.1"
                     /translation="MSEYVLLYILFAPFVGVTALIFISNRQLMLVRGVAATSAGICLL
                     ASLYLFFAYDAARGGFQFVQRFEWSRQLGISLYLGVDGIGTPLVLASSILLFAGIFVS
                     WHIKDRTKEFYIWLLILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     NEMTDPEGLKQRDSVGFILNFASNSKEYAAMKLVLFLSAFAVVALMGILLIYKYSGVN
                     TFDILVLREQAKLMNIPVLGTTLDKIIWVLIFFGFASIAPLWPLHSWSPVGHAAAPAA
                     TSMLHAGVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCMFSIIYGGFVAFYAKDTK
                     YVIGYSSSSHMGYVFLGMAALNYIGLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRD
                     IPSLGGLSNRMPFIAGCFVVACMASIGMPGTVNFIAEIMIIVGSWEKYPLQVIVAVIG
                     IVITLAYLFRMMRGVFYGTMDQKYSHSHDAVAVADRLPLLVMIAVSIGFGLFPMHLYN
                     VVRSGVDPLVARITHVVPIAAEATGTRHGGRGDDGGTSTAQLLPRSTSHESGDRP"
     gene            complement(4180..5739)
                     /locus_tag="VI359_00250"
     CDS             complement(4180..5739)
                     /locus_tag="VI359_00250"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6972709.1"
                     /translation="MLEELASAFPILSCILFLPVAGAAVLWLFDDEDMIRTSALTIAL
                     VELALSLFVLLRFVPDSAAMQFAEHVRWIPALGISYHLAVDGISVLFVGLTAFLTVLI
                     VIYSWDTVRHQVKLYMMALLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDLNYHQWATLHHMEPSYSFDLLELLTVPI
                     PLHQQILVFWLMFLGFAFKAPVFPFHTWLPDALLEGPIGMAVVLAGLKLGTFGFIRFS
                     IPLLPEASKSQMVVSIVMALGLAAILYGAIMALIQPDFRRLLAYSSISHLGFIVIGLF
                     ALNYQGLQGSLLTMINLGFSTAGLFFIAGFLYSRQQTTQLASFGGMAKQVPLLATFFL
                     FIGLASIGLPGTNGFVGEFLILLGTFKAHWLYGSIAVLGVIFGAAYFLWYYERAMLGP
                     LSKTVRESIGDLHFREVVIALSLSVMILWIGLYPAPFLKMMNGSVQALVDRLDRGTVA
                     SMNNASTVRTD"
     gene            complement(5749..7719)
                     /gene="nuoL"
                     /locus_tag="VI359_00255"
     CDS             complement(5749..7719)
                     /gene="nuoL"
                     /locus_tag="VI359_00255"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY6972710.1"
                     /translation="MPLTDWLILLIPVFPLVAVIINGLLGSRYSHDWAYWLACGSVGA
                     SFLCTIGVFGDILKTGTPHEVIAYQWIFGGDLTINLAYLVDSLTCIMLLVVTGVGFLI
                     HVYSVGYMHGETGFTRFFTYMNLFMVSMLLLVMGNNYAVLFIGWEGVGLCSYLLIGYY
                     YERVSAAKAATKAFVVNRIGDAGFLLAIFLVFINFKTLDYTKVFAQVSQLPPEMATAI
                     AICLLVGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIYDLS
                     PTSMTVVGIIGGSTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGIGAYTAAIFH
                     LMTHAFFKALLFLSAGSVIHALSGEQDIRKMGGLKHKIPWTHWLFLIGTVAIAGIPPL
                     AGFWSKDEIMAHAFTHHQYLLYGMAAAGAFLTAFYMFRLTYLTFYGPSRMDHHTEEHV
                     HESPMVMIWPLAILGGLSLVGGFLGVPPEHGWFQRFLASVAGTGSGHEAAVGIVLTLM
                     SIATGIALFGWALAHYFYSVSPATPERWATKFSTVYTTLLNKYYVDELYDVLFVEPTK
                     KLGWLFDWFDRIVIDGIVRAVGQAAELGAAGSTWFEKHVIYGGINIIGYGNHLAARQW
                     RHLQSGMVQHYAAMIVAGLFLLALVIQLVLQL"
     gene            complement(7724..8026)
                     /gene="nuoK"
                     /locus_tag="VI359_00260"
     CDS             complement(7724..8026)
                     /gene="nuoK"
                     /locus_tag="VI359_00260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY6972711.1"
                     /translation="MVPLSAYVAVSAILFLTGLLGVLIRRNFIIVLMAVEIMLNAANI
                     NLVAFSHYMESMAGQLVALFIIAIAAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            complement(8102..8626)
                     /locus_tag="VI359_00265"
     CDS             complement(8102..8626)
                     /locus_tag="VI359_00265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY6972712.1"
                     /translation="MTLVFFAYFALVSIAAGVLTVALRNPVHCGLALLALLLHVSGLF
                     VLLNAEFLWAVQVVVYAGAILVLYLFVLMLLNLKTEERYFHSTFMYFLAPALIGSLYV
                     LALLFRSPFGGAKGDAPAAAILQDGDTHAVGIKMFSDYLLQFEIVGVFLLGAIIGAIV
                     LAKTPKPLDVERER"
     gene            complement(8634..9221)
                     /gene="nuoI"
                     /locus_tag="VI359_00270"
     CDS             complement(8634..9221)
                     /gene="nuoI"
                     /locus_tag="VI359_00270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY6972713.1"
                     /translation="MSVVTLTKKVLQAALFFEIWDAMKVTFKHMFHKPITFQYPREQR
                     IIPDTHRGALGLLRYDDGRERCVGCDLCEAACPSRCIKVISAEDNTRPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEYSTHDKRTLLFDKARLYDIGERHLEDAKKYL
                     YAHNQEKNVEESREYRYYFPQSVLKATQPSPKHLS"
     gene            complement(9238..>9430)
                     /locus_tag="VI359_00275"
     CDS             complement(9238..>9430)
                     /locus_tag="VI359_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY6972714.1"
                     /translation="VRVTSDRGSLQLGVQPDQSVAPHTCFFPEHFNEPPVKDLMSIQL
                     DPVTNVPSFKLTRVTIEKA"
BASE COUNT         2390 a         2560 c         2703 g         1777 t
ORIGIN      
        1 aggggcgcgc gcagatcatg cgagacggaa tagctgaagg cctcgagttc cttgttggcc
       61 gcttggagta actctccgcg gcggcgaagt tctgctgcga cgcgacgtcg ctccgtgaca
      121 tcgtgatgga tcaggtaata cacggaggcg agcaaaacga gctgcaggag cgcaccgagg
      181 accaggagca cgatggtgtt tctcgtgctg cctgtcgatt gaatgacgcg gctccgaagg
      241 gccttctgct cagtcgtttc cagttcgacg atcaagcgat ggatggcgtc gagttcttgt
      301 ttcgctgcgc cggagagggc gatacggcga accccctcgt agccgtttgt ctctcgtgct
      361 gaaatagcgg cggcctcggc atcgagttgt tgaccgatta atcgttcaag gctcgccacc
      421 cgttcttgtt ggtcggaggt tccttctgtc aagcctcgga gatactgaga gaagacgggc
      481 atctgctcga ggactgtctt gtgtcctttg aggtaggcct cgtcgccggt cacaagatag
      541 cggcgatgcc cgcgctcggc atcatccaat gcctcgccga tcgaacctag aagttgcacg
      601 agttcatggc ttctcgtatc gagacggctg ttcttgatca gcacggacgt gtttcgatac
      661 gacgcgatgc tgatcacgac gattcccgca aacacgagtc caaacccagc gagcacgcgc
      721 tgctcgatgg accagttggc gaaccatcgc acagctctag ttacgcgcga gggaggcgag
      781 gatgtcttct cggggcccga cgttgatggc acgggcgccg tcggggctga aacggtaggg
      841 cggtcttgtg tgacggatgc catgatagat gtagaaccca caccgcggcg ttaggggaaa
      901 acccgtgacg gaactgtact ctgccccact agaccctgca agaaatattt taccagaagt
      961 cccgctgggc gcggcgtgac gaagaggaaa gattatccac cgaaaggcga cgggggcgga
     1021 atggacgccg ctggaccggc aatgttggag aacatcagcg ttgcggccac tacccagtcg
     1081 atcaccggtt gtggatagat gccgatcgcg agcgtccccg cgagtccgac gtataccacg
     1141 gccttcatgg ggcccgagat tgacaccggt gacgggtcgt gaggctcgat gatgtacatc
     1201 ttcttcacga cgatcagata gtaatacatg gagatgacga tgttgatcag tccgaccgtg
     1261 atgagcgtat agagtccttc tttgatcgct gcgacaaaaa tatagatttt gccgatgaat
     1321 cctgcgagtg ggggcacgcc ggccagagac aacagaaaaa tcagcatggc gaaagcgaga
     1381 aacggcgacc ggcggccaag tccgctgtaa tcctcgattt catcgctgcc gatcgcctgg
     1441 ctcaccgcaa tgacgatggc gaaggctccg agatttgcga acaggtaggt cagcaaataa
     1501 aacaagatcg catcacttcc catcttcgtc ccagcggcga ggccgatgag cacattgcca
     1561 atctgagcta tgccagaata ggccagcagt cgtttgatgt tcttctgcgc gattgccacg
     1621 atgttgccat aggtcatcga cagaatcgag gccgccacca gcatgaggac ccaggtgggt
     1681 ttgaagctgg cgagcgcgac gtagaacatc cgaaggagaa tggcgaacgc tgcccctttg
     1741 ggtgcaatcg acagaaatgc ggtcaccggt gtcggtgatc cgtgataggt gtcgggaatc
     1801 caggaatgga atggcacagc cccgattttg aatcctagtg cggcgaagat aagcaaataa
     1861 ccgataatca accccggcgt agcctgtgca gacgtcatat ccgagaagac gagtttcccc
     1921 gtctcgccgt agaccaagct gattccatag gcaagaaggc cggccgcaaa aacacccagg
     1981 atgaagaatt tgagccccgc ttcgtttgac gcggcgtctt cccgcaagta ggccaccaaa
     2041 acatagaacc cgaatgttga gaactcgagg gtcacaaaga cggacaggag atcgttcgcc
     2101 gaggccatga acatcatacc cagcgccgac atcgcgacca gaaaataata ttccccgcgg
     2161 aaaaacgtga aacgacgaac atattcgatg gaggcgatga tgactaatat ggtggccagc
     2221 acgataagca tcttgaagaa caaggccatg cggtcgacca caaacatgtt ggcaaagagc
     2281 gttccggtga tgccggtcac atcgaaccat gcgaggcatc ccagcgtgat tgcgagccct
     2341 cctatactga gataggccag ttgttccttc ggcagccggg gaaaggcaaa atcgacgacc
     2401 agaatcaggc agatccagaa cgtgaggagc atctccggca ggagcaacag gagatcggat
     2461 agcgacatgg ccagcgagaa gttcatggac gatctccgga ctcgtggctg gtggagcggg
     2521 gtaatagctg ggcggtactt gttccgccgt catcgcctcg ccccccgtgc ctcgtgcctg
     2581 tcgcttccgc ggcgatcggc accacgtgag tgatcctggc gacgagcggg tcgacgccgg
     2641 atcgaaccac gttatacagg tgcatgggga acaacccgaa cccgatgctg acggcaatca
     2701 tcaccagcag cgggaggcga tcggcgacgg ccacggcatc atgcgaatgg ctatactttt
     2761 gatccatcgt cccgtagaaa accccgcgca tcatccggaa caaataggcc aaggtgatca
     2821 caatgccgat caccgcgacg atgacctgca agggatattt ctcccaactc ccgacgatga
     2881 tcatgatttc ggcgataaaa ttgacggttc ccggcatccc gatcgatgcc atgcaagcca
     2941 ccacgaagca gccagcgatg aagggcatgc gatttgacag cccacccaac gacgggatgt
     3001 cgcgcgtgtg ggtctgatcg tacacccatc cggccatggc gaagagcatg ccggttgcca
     3061 tcgcgtgggc gaacatataa atcaccgcgc cgctcagtcc aatatagttc aatgcggcca
     3121 tgcccaggaa tacgtatccc atgtggctgg aactcgagta gccgatgaca tatttcgtat
     3181 ccttggcata aaaggcgaca aatcccccat agatgatact gaacatgcag agcactgcgg
     3241 cgatcggcat gagctctctg gtcgtttcgg gaagaatttc gaacgccaca cggatgatcg
     3301 aaaaatgccc cagcttcatc agcacgcccg cgtgcaacat gctcgtcgca gcaggggcag
     3361 ccgcgtgacc gacgggggac caggagtgca gaggccagag cggtgctatg gaggcaaagc
     3421 cgaagaagat cagtacccaa atgatcttgt cgagagtcgt cccgagcacc gggatattca
     3481 tgagcttcgc ctgttcccgc aagaccagaa tatcgaaggt attcacgccg gagtacttgt
     3541 aaatgaggag gatgcccatc agggcgacca ccgcgaaggc ggataagaag aggacgagtt
     3601 tcatcgcagc gtattctttg ctgttcgaag caaaattcaa aatgaagccg acggaatccc
     3661 gctgcttgag accttctgga tctgtcatct cgttgtattt tttcgtgtga cttccccaca
     3721 tgcccaagag tagatacatc gggatgacgg acatttcgta gaagaagtac aagaagaata
     3781 aatccaacga catgaacacg ccgatcgtcg cagcggccaa gatcagcagc caaatataga
     3841 attctttggt gcgatctttg atatgccagg agacgaagat gccggcgaac agcaggatcg
     3901 acgaagccaa taccagcggt gtcccgatgc cgtcgacgcc aaggtagagg gagatgccga
     3961 gttgcctgga ccattcgaag cgttgcacga actgaaaccc gcccctcgcg gcatcgtagg
     4021 caaaaaacag gtagagtgac gcgagtaagc agatgccggc cgatgttgcc gcgactccgc
     4081 ggacgagcat caattgccga ttcgagatga agatgagcgc agtcacgccc acgaacggcg
     4141 cgaacaaaat atagagaagc acgtattcac tcatacgatt cagtccgtcc tgacggtgga
     4201 tgcgttattc atggaggcga ccgtcccgcg atctaaccga tcgaccaaag cctgcaccga
     4261 tccgttcatc atcttcaaga acggggctgg atagaggccg atccacagaa tcatgacgga
     4321 caacgacaat gcaatcacga cttcgcgaaa atgaagatcg ccgatcgact ccctgacggt
     4381 tttacttaac ggaccaagca tcgcccgctc gtagtaccac aagaagtatg ctgccccgaa
     4441 gatgactccg agcaccgcga tggaaccata gagccaatgg gccttaaatg ttcccaggag
     4501 gatcaaaaat tcccctacga atccgttggt gcctggtaga ccgatcgagg cgagaccgat
     4561 gaacaggaaa aacgtcgcca acaagggtac ctgtttggcc ataccaccga acgacgcaag
     4621 ctgagttgtc tgctgtctcg aatagagaaa cccggcgata aagaacagtc ccgccgtact
     4681 gaatcccaga ttaatcattg tgagcaggct gccctgaaga ccctggtagt tgagcgcgaa
     4741 gaggccgatc acgatgaagc ccaggtggct gatactgcta taggctaaca ggcgacgaaa
     4801 atccggttga atcagcgcca tgatcgcgcc gtacagaatg gcggccaggc caagcgccat
     4861 cacgatcgag acgaccatct ggctcttcga cgcttccggc agtagcggga tgctgaaccg
     4921 tatgaatccg aacgtgccca atttcaaccc ggccaggacc acggccatac cgatcggacc
     4981 ttcgagcaac gcatccggca accatgtatg gaacgggaac acgggagcct tgaaggcgaa
     5041 gcccagaaac atcaaccaga agaccagaat ttgctgatgg agcggaatcg gtaccgtcaa
     5101 tagctcaagc agatcgaagg aataggacgg ctccatgtga tggagcgtcg cccactgatg
     5161 atagttgagg tccaggaggg caatgcccac cagcatgaag acgctgccga gcaaggtgta
     5221 gagcacgtat ttcagcgccg catagtgtcg ctctgcccct ccaccccaga gcttgatgag
     5281 aaagtagctg gggatgagca tcaactccca aaatacaaaa aagaggatga ggtcgaggga
     5341 gacaaagacg cccatcgtcg tggtttcaag tgcgagcagc gccatcatgt agagcttgac
     5401 ctggtgacgt acggtgtccc acgaatagat gacaatgaga accgtgagga aggccgtcag
     5461 tccgacgaac agaacgctga tgccatcgac cgccaggtga tagctgattc caagggctgg
     5521 aatccatcgg acatgctcgg caaactgcat cgcggccgag tcgggaacga accggaggag
     5581 gacgaagagg gacagggcta gttcaaccaa tgcgatcgtc agcgccgagg tacggatcat
     5641 gtcctcgtcg tcgaagagcc acaggacggc agctccggca accggtaaaa agagaatgca
     5701 cgagaggata gggaatgctg atgccagttc ttccaacatc gcgtaactct agagttgcag
     5761 caccagctgg atcaccaggg cgagcaagaa taggcccgcc acgatcatcg ccgcgtagtg
     5821 ctggaccatc ccgctttgca aatgtcgcca ttgtctggca gcaagatgat taccgtaacc
     5881 gataatgttg atgccgccat atatcacgtg cttttcgaac cacgtcgagc cagccgcccc
     5941 cagttctgca gcctgaccga cggcccggac gattccatcg atcacgattc ggtcaaacca
     6001 atcgaacagc cagcccagct tctttgtcgg ttcgacgaag agcacatcgt acagctcatc
     6061 gacgtaatat ttgttcagca gggtcgtgta gacggtcgag aattttgtcg cccaccgttc
     6121 tggcgttgcc ggactcacgc tataaaaata atgagcaagc gcccaaccaa agagcgcgat
     6181 acctgtggcg atgctcatga gtgtcagaac aattccaacc gccgcctcat gaccgctccc
     6241 cgtgcctgcg actgaggcaa ggaaacgctg gaaccacccg tgctctggcg gaactcccag
     6301 gaaaccgccg acaagcgaca ggcctcccaa gatcgccaac ggccagatca tgaccatcgg
     6361 cgactcatgc acatgttcct ccgtatgatg gtccatccgt gatggcccgt agaaagtcag
     6421 ataggtcagt ctgaacatat agaaggccgt caagaatgcg ccggccgcag ccattccgta
     6481 cagcagatac tgatggtgtg tgaatgcgtg ggccataatc tcgtctttgc tccagaatcc
     6541 cgcgagcggc ggaatgccgg cgatggcgac ggtgccgatc aggaagagcc aatgagtcca
     6601 ggggatcttg tgtttcagtc cgcccatctt ccgaatgtcc tgttcgcccg agagcgcgtg
     6661 aatcacggat ccggcggaca ggaacagcag tgccttgaag aaagcatgcg tcatgagatg
     6721 gaagattgcc gcggtgtagg cgccgattcc gcagccgaga aacatatagc ccagttggct
     6781 gaccgtcgaa taggccagga cgcgcttaat atcggtctgg actaaaccga tggtggcggc
     6841 aaagagggcc gtggatccgc cgatgatgcc gaccacggtc atggaggtcg gcgagagatc
     6901 gtagatcgca tggtttctga caatcatata gacgccggct gtcaccatgg ttgcggcatg
     6961 aatcaaggcg ctcactgggg ttggaccctc catggcatcc ggcagccagg tgtagagagg
     7021 aagctgtgcg gatttcccga cggcgcctac gagcaagcag atcgcgatcg ccgtggccat
     7081 ctccggcggc agttggctga cttgggcgaa gaccttggtg taatccaagg tcttgaagtt
     7141 gatgaaaaca agaaagatcg ccaacaggaa gccggcatcg ccgatgcggt tcacgacgaa
     7201 ggctttcgtg gccgctttgg ccgcggagac tctctcatag tagtagccga tcaagagata
     7261 ggagcaaagc ccgacgccct cccagccgat gaacaaaacg gcatagttgt tgcccatcac
     7321 gaggagcaac atggaaacca tgaatagatt catataggtg aaaaaccgcg taaatccggt
     7381 ttcaccatgc atgtagccga ctgaatagac gtgaatgagg aagccgactc ccgtcacgac
     7441 cagcagcatg atgcaggtga gggaatcaac gaggtacgcg agattgatcg tgaggtcgcc
     7501 gccgaagatc cattggtagg caatgacctc atgaggcgtg ccggttttaa ggatgtcgcc
     7561 gaagaccccg atggtgcaga ggaaggaggc accgaccgac ccgcacgcca gccaatatgc
     7621 ccagtcatgg gaataccggc ttcccaagag gccattgatg atcaccgcta ccagcgggaa
     7681 gaccggaatc agaagaatga gccaatcagt cagaggcacg gcgttaccac ttcaacagat
     7741 tcatttcatc cacgttcgtc gagatctttc cccggaagac gacaatgatg atggcgagtc
     7801 cgactgctgc ttctccggcg gcgatcgcaa tgataaagag cgccaccagc tgtccggcca
     7861 tcgactccat gtaatgcgaa aaggcgacga gattgatatt ggccgcattc aacatgatct
     7921 cgactgccat caatacgata atgaaattgc gcctgatcaa gacgcccaat agtccagtga
     7981 gaaacaggat ggcactcacg gcgacatatg cgcttaatgg aaccatcgtt gccctaccgt
     8041 tctatcgcca gtagacatcg atcgaagcga ggacaaaact cgcgtaccga cagccgaccg
     8101 gtcatcgttc tcgttccaca tccaatggct tcggcgtctt cgcgagcacg atcgcgccga
     8161 tgattgcgcc caagaggaaa actcccacaa tttcgaactg caggaggtaa tcgctgaaca
     8221 tcttgatgcc gaccgcgtgg gtgtccccat cctgcaaaat cgccgcggct ggcgcgtctc
     8281 ccttcgcacc gccaaacggg gagcgaaaca agagcgcgag cacataaagc gatccgatca
     8341 gtgcgggagc caagaaatac atgaaggtcg aatggaagta gcgctcttcg gtcttcaggt
     8401 tcaacagcat caaaacgaag aggtacagca cgaggattgc cccggcatag accacgacct
     8461 gcactgccca gagaaattca gcattcaaga ggacgaagag cccagacacg tggagcagga
     8521 gggccaagag ggccaagccg caatgcaccg gattgcgcag cgccaccgtg aggactcccg
     8581 cggcaatact cacgagcgca aagtaggcga agaagacaag ggtcatcgag ggttcagctc
     8641 aaatgtttgg gagacggttg ggttgccttg aggacggatt gtggaaagta ataccgatac
     8701 tctcggctct cttcgacgtt cttttcctga ttgtgcgcat agaggtactt ttttgcgtcc
     8761 tcgagatggc gttcgccgat atcgtacagt cgtgccttgt cgaacaacag agtacgcttg
     8821 tcgtgggtgg aatattcgta catctttgtc atggcaaggg cattcacggg acaggcttcg
     8881 acgcagtagc cgcaaaacac acacttggta atgtcgatat aaaactcgct ggcgtatcgc
     8941 tgtaatggcc gggtattgtc ttccgcactg atgaccttaa tacatcgcga tgggcaggcg
     9001 gcctcacaga gatcacagcc gacacatcgt tcacggccgt cgtcgtatcg caaaagtcca
     9061 agcgccccac ggtgtgtgtc gggaattatc cgctgttccc gaggatactg gaacgtgatg
     9121 ggcttgtgga acatgtgctt gaacgtcact ttcatcgcgt cccagatctc aaagaacaac
     9181 gcggcctgca gaactttttt ggtgagagtg acgacgctca tcagcttctc cgtgccccta
     9241 ggccttttcg atcgtgacgc gcgtgagctt gaacgaagga acgttggtca cggggtcgag
     9301 ctggatcgac atgagatcct ttaccggtgg ttcattgaag tgctccggga aaaaacaggt
     9361 gtggggcgcc accgactgat cgggttgcac gcccaactgg agcgacccgc gatctgaggt
     9421 gacgcggacc
//
LOCUS       DATDNW010000011         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1299007, whole genome shotgun sequence.
ACCESSION   DATDNW010000011 DATDNW010000000
VERSION     DATDNW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299007"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1245
                     /locus_tag="VI359_00280"
     CDS             91..1245
                     /locus_tag="VI359_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6972715.1"
                     /translation="MPSTIFVIDSSPAVRRMVEQISTPEGFEVVGFQDGPTALEAARK
                     KSPHLIIADYHLDNMTFSGFCKEVHKIDNLADTYIVSLISPSDRLDEKHLRSLGVKAF
                     LKKPFQSENLLEVIKDLGTNGTKKRRAWPPTSSATDSDDDPSLHDQDDESELSEEDTG
                     QIAAVQIPPSTISKKSTAPPRPTAAAPVAQKSPGKPEDAMKGHSDPLMQWMSERTEDI
                     IATMLPQVISKELASQVAKAVQDEVQTQLGATLSQERLAHMIEPLLAKELSNVLSREM
                     PVLEPIIRHSIFEIATPLVKDSIDELAREQAETVKKTLPDVVHDQLGSIDVLVRDEIQ
                     QAAVKQATHIADEIVRAAVKEQIEQAVQRLVPGIAEEQIKAELKRLTATE"
     gene            1543..>2310
                     /locus_tag="VI359_00285"
     CDS             1543..>2310
                     /locus_tag="VI359_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6972716.1"
                     /translation="MATPQLDKTYDPKAVEARWYQLWEQRGYFHASVTHPGQPYCIVI
                     PPPNVTGSLHVGHALNNSIQDILIRWRRMQGRNVLWMPGMDHAGIATQNVVERQLAVE
                     GTSREQLGREKFVERVWAWKAQSGGIIIQQLKRLGASCDWDRLRFTMDEGLSKAVLEV
                     FVRLYEDGLIYRGERLINWCPRCLTALSDIEVEHEEIKGKLYSIEYPLEHDLAIHLTV
                     ATTRPETMLGDTAVAVHPEDPRYNRLIGQRVRLPLTNR"
BASE COUNT          554 a          686 c          601 g          469 t
ORIGIN      
        1 acgtcaaagc gaacacgaat tagtggtttt cttgacaaag aataccctcg ctgctagctt
       61 cgacatagtt caataaaatt ggagacttag gtgccttcaa ccatttttgt gatcgacagc
      121 agtccggctg ttcgccggat ggtcgaacag atctcgacgc ctgagggctt cgaggtcgtc
      181 ggctttcagg acggccccac cgcattagag gcggcccgca agaagagccc acatctcatc
      241 attgccgact atcacttgga caacatgact ttctcgggct tctgcaagga agtccacaag
      301 atcgacaatc tggccgacac gtacatcgtc tccttgatca gcccatctga ccggctggac
      361 gaaaaacatc tccgttcgtt gggagtcaaa gcgtttctca agaaaccgtt ccagtctgag
      421 aacctgttgg aagtcatcaa ggatctcgga acgaacggaa caaagaagcg ccgtgcttgg
      481 cctccgacat cgagtgccac tgattcagac gatgacccct cgctgcatga tcaggatgac
      541 gagagtgaac tgtctgaaga ggacacgggc cagattgccg ctgtccagat acctccatca
      601 acgatctcaa aaaaatcgac agccccgccc cgaccgacag cagctgctcc agtcgcacag
      661 aaatcaccgg gcaaacccga agatgccatg aagggccatt ctgacccact gatgcagtgg
      721 atgtctgagc gcaccgaaga cataatcgcg accatgctcc cgcaagtcat cagcaaggag
      781 ctggcatcgc aggttgccaa agcggtccag gatgaagtac agacccaatt aggcgccacc
      841 ctctcgcaag aacggctggc acacatgatc gaaccgctcc tcgccaagga attgtccaat
      901 gtgttgtccc gcgaaatgcc ggtgctggaa ccgatcatcc ggcacagtat ctttgaaatc
      961 gcgaccccgc tcgtgaaaga cagcatcgat gaacttgcgc gtgaacaggc cgagacggtc
     1021 aaaaagacac tgcccgatgt ggtccatgat caacttggct caatcgacgt gctggtacgg
     1081 gatgagattc agcaagctgc ggtcaaacag gccacccaca tcgccgatga gatcgtacgc
     1141 gcggcagtca aagaacagat tgaacaggcc gttcagcgcc ttgtgcccgg catcgccgag
     1201 gaacagatca aggctgaatt gaaacgcctc accgccaccg agtagcgaga cagcttctga
     1261 gatcccttac cccttctttc ccttcttgct ccgacgtgct ttcgccaacg ccttcatacg
     1321 gttgatattc ttccggtgct ttttccgagt ttttctatct ttgagccgtc ccgtcgccat
     1381 agttgctcca tcctggtgtg aataaaataa tccgcgccgt cgcaacaatt cgcaagcttg
     1441 tataacacaa gccatgaggg cccacaagcc gcgcggccat tcgcttgtga gcctgcaccc
     1501 tgcatgttaa gatgcgcgcc gctcaaccct cgttggtagg cgatggcaac cccgcaactc
     1561 gacaagacct acgatccgaa agcagttgaa gcgcgctggt atcagctctg ggagcaacgc
     1621 ggctattttc acgcgtcagt cacccacccg ggccagccat actgcatcgt catccctcct
     1681 ccgaacgtca ccgggtcctt gcacgtgggg catgccctca acaactcgat ccaggatatt
     1741 ctgattcgct ggcgtcgcat gcagggacga aacgtgctgt ggatgccggg gatggaccat
     1801 gccggcatcg ccacacagaa tgtcgtcgag cgacaactcg ccgttgaagg cacgtcgcgc
     1861 gaacagcttg gtcgggagaa atttgtcgag cgggtgtggg catggaaagc ccaatcaggt
     1921 ggcatcatta tccagcaact gaagcggctc ggcgcctcct gcgactggga tcgactgcga
     1981 ttcacgatgg acgaaggctt gtcaaaagcc gtcctcgaag tctttgtccg tctctacgag
     2041 gacggtctga tctatcgtgg cgaacggttg atcaactggt gcccacgctg cctgactgca
     2101 ctctctgata ttgaagtcga gcacgaagag atcaagggga aactctattc tattgagtat
     2161 ccgctggagc acgacctagc aattcatctg accgtcgcga ccacccgtcc ggagacgatg
     2221 ctcggcgata cagcagtggc cgtccatccc gaggatcccc gctacaatcg cctgatcggt
     2281 caacgagtgc gcctgccgct cacaaatcgg
//
LOCUS       DATDNW010000012         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_479691, whole genome shotgun sequence.
ACCESSION   DATDNW010000012 DATDNW010000000
VERSION     DATDNW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479691"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VI359_00290"
     CDS             complement(<1..407)
                     /locus_tag="VI359_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6972717.1"
                     /translation="MSEQSFVSNLPVLPIKRTVLFPGVMMPLTVGRDRSIAAVEAAMR
                     TEDKTLLVIAQRVPDVDGPELKDLYSIGTKAVIKQIARVSEGQIHAMVQGLERVVLLR
                     LDQTDPYLVARVREYPEPTDTGTEVDALHRAIQ"
     gene            complement(586..2604)
                     /locus_tag="VI359_00295"
     CDS             complement(586..2604)
                     /locus_tag="VI359_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972718.1"
                     /translation="MSGRLWRLLSLLLLLVIPGCSAVQYFFELIGPPSAPHSHMRESV
                     VDRIGPPIRNELERLVGARQTAVLAAHETIETNPARFKRRLAVAALKHPWEGLADLER
                     QGLLIAEMAEGGPVNLPALLDILEAGMDRTSSFHKAVPLPSGTTPAELVTFMVDSLEA
                     ASTLRDKALANLTEEERHFLFTHAATLVERFTPQISSLTDQTIPFIKANLRFGELLDE
                     QVDYAALIAAAQVLARFANERWLHQVTTAFSKPLLPTQVPPGVTGDVLLVEKTSYGMI
                     IVGGAGPNTYDLDGRFGLVIDLGGNDLYRGTIAASASEDQGNAVVIDLSGNDTYDGAP
                     LGLATGRLGVGLLIDHAGDDVYQLERGSGGAGFGGLGILFDGKGNDAYMGNRLTQGAA
                     IGGLGLLLDSAGNDRYTSYGFAVGFGGPLGVGALIDVTGDDHYQCGDKYPSAYNAQDA
                     PNGKPGDPLFQYDCFGLGTGSGQRILTKRLEWQAHNLAGGWGILLDIDGHDHYQSANF
                     SQGHGYFFGTGLKLDLDGDDDHQAARYGHGASAHFGAALFIDQHGEDHYGSSGPLYNA
                     GVAWDHSAGFMIDGGKGRDIYLFDHSTGLGMADLTSWSVFIEEGGADQYRVSRGMGAA
                     DTTSLGAFFDLAGTDVYPDLPTTNSSGTLKRTNNTIQQDTAGGLFVDK"
BASE COUNT          604 a          794 c          795 g          601 t
ORIGIN      
        1 tcttgaatcg cgcgatgcaa ggcatcgacc tcggtgccgg tatccgtcgg ttctggatac
       61 tcacggacgc gagcgaccaa gtacggatcc gtttgatcga gcctcaggag aacgactcgt
      121 tccagccctt gaaccattgc atggatttgc ccctccgata cgcgggcgat ctgtttgatg
      181 acggccttgg tcccgattga gtacagatct ttcaactctg gtccatcaac atccggcaca
      241 cgttgggcga ttaccaggag cgtcttatct tccgttctca tggccgcctc gaccgcggca
      301 atggaccggt ctcgaccgac ggtcaacggc atcattacgc ccggaaacag aaccgtgcgc
      361 ttgatgggaa ggaccggcag attcgagacg aacgactgtt cactcatttt ggcctccttg
      421 gggactatac tgaatgtgtg attgttaagg tagccatcac ttgaggtgag tcaaggtaag
      481 ggagagatcg ctcgccggat catgttgaac acactgactg gaagagtcgg agcctgcttg
      541 aatcgataca ggagatttag gctctatcac tttgaaactg ttggactact tatcaacgaa
      601 caacccgcca gcagtgtctt gctgaatggt gttgttggtg cgtttcaatg tgccagaaga
      661 attcgtggtt gggaggtctg ggtagacatc cgttcccgcg agatcgaaga aggcgcccag
      721 gctcgtggtg tccgctgcgc ccatcccccg tgagactcga tactggtcag cgcctccctc
      781 ctcgatgaag acgctccacg aggtaaggtc agccatccca agaccggtcg agtggtcgaa
      841 cagatagatg tcacggccct tgcccccgtc gatcatgaac cccgcgctgt ggtcccaagc
      901 cactccggcg ttgtacaagg gacctgatga tccataatga tcctctccat gctggtcgat
      961 gaagagggcg gcaccaaaat gagccgaggc tccatgtccg taccgagctg cttgatgatc
     1021 gtcgtcgcca tccagatcga gtttcaggcc tgtgccgaag aaatacccgt gtccttgtga
     1081 gaaattggcg ctctgatagt ggtcgtgacc gtcgatgtcc agtagaattc cccacccgcc
     1141 ggccaaattg tgggcttgcc attcgagtcg cttggtcaaa atgcgttggc ctgatccggt
     1201 gccgagtccg aaacaatcgt attgaaacaa tggatcaccg ggtttcccat tgggagcgtc
     1261 ctgtgcattg taggcgctgg ggtacttgtc accacactgg tagtgatcgt caccggttac
     1321 atcgataagg gcgccgactc ctagcggtcc accgaatcca acggcgaatc cgtagctcgt
     1381 gtatcgatcg ttccccgcgc tgtccagcaa caaaccaagg cctccaatgg cagcaccctg
     1441 ggtcaatcga tttcccatgt acgcatcgtt gcctttgccg tcgaataaaa tcccaagccc
     1501 gccgaatcct gcgccaccgg atccacgttc gagttgataa acgtcgtcac ccgcatggtc
     1561 aattaaaagg cccacaccga gtcgcccggt ggccagtcca agcggcgcgc cgtcataggt
     1621 atcgttccca ctgagatcga tgaccacggc gttgccctga tcttcactgg cagaagcggc
     1681 aatcgtccct cggtagagat cgtttccccc aagatcgatc acaagaccga atcgcccgtc
     1741 gaggtcatac gtattcggcc cggccccgcc gacgattatc atgccgtagg aggtcttctc
     1801 gaccagcagc acgtcgccgg tcacgcccgg gggaacttga gttggcagca gcggtttgga
     1861 aaatgcagtg gtgacctgat gcagccaccg ttcgttggcg aagcgggcga ggacctgagc
     1921 agccgcaatc agagcggcgt aatcgacctg ttcatccaga agctcaccaa accggaggtt
     1981 ggctttgatg aaggggatcg tctggtcagt cagacttgag atctgcggtg tgaatcgctc
     2041 gaccaatgtt gcggcatgcg taaagagaaa atgtctctct tcttccgtca ggtttgccag
     2101 cgctttgtca cgcagggtcg atgctgcctc taaactgtcg accatgaagg tcaccagttc
     2161 agccggtgtg gtgcccgagg gcaaggggac cgccttgtga aatgaagagg tgcgatccat
     2221 tcccgcttcg agaatgtcga gcagagccgg gagattgacc ggaccccctt cggccatttc
     2281 ggcgatcagc agcccctgtc gctcaagatc tgccaggcct tcccaaggat gtttgagcgc
     2341 ggcgaccgcc aaacgccgtt taaagcgcgc tgggttggtt tcgatcgtct catgggctgc
     2401 cagcaccgca gtctgacgag cgcccaccag ccgttcgagc tcgttgcgga tgggtggccc
     2461 gatgcgatcg accaccgact cccgcatgtg gctatgggga gcagacggtg gaccgatcag
     2521 ttcaaaaaag tattgaaccg cactacagcc ggggatcacc aacaggagga gaagactcag
     2581 aagtcgccat aaccgtccgc tcatagaagt ctgtacagca taccgtgggg ggttacccat
     2641 tgctacgggt ggtgcgctga acctttcgca ttgatccctc aacgatccgg aattcataga
     2701 gacgacattc caacgatccg ttaaacagag ggattcgttt tgagggggac aggccgatca
     2761 gcttcgggac gcgactgtcg ccggtgaaga cgta
//
LOCUS       DATDNW010000013         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_616313, whole genome shotgun sequence.
ACCESSION   DATDNW010000013 DATDNW010000000
VERSION     DATDNW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616313"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VI359_00300"
     CDS             complement(<1..465)
                     /locus_tag="VI359_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6972719.1"
                     /translation="MNVRGFWIGLAVGIISTCLFGQWVVSAAIREASSPKCIPAESVA
                     NYVHSVIQADRTFYTTDIVERMQMRGIVVASENWRETARLPLPAQFLIEAGQLVAAER
                     SGLRFRLISNWAINKKNRPTTEFEQTGLTEILVNPDRPYTGVTTEGGTPVFQA"
     gene            complement(746..1405)
                     /locus_tag="VI359_00305"
     CDS             complement(746..1405)
                     /locus_tag="VI359_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972720.1"
                     /translation="MDARPRIERSAFLAATAAMVSLWMDTACRPTPPIAVDQAPANLA
                     IIGFPVPQHVRRLAVWYPNTSEQDVAYGYSRLEQAAFQLKTQRSWIKIVERRNIGPLT
                     DEQRLQLSGRVGDDSAVRVGKWLGADSIILFRIDMPTWRERLLARFYGKMPPFVVSSK
                     IISVESGEVLYHDIVTAMPVPHSGAWSDYASDYELQPALRSALDHALSVAIVHLNQSF
                     R"
     gene            complement(1671..2021)
                     /locus_tag="VI359_00310"
     CDS             complement(1671..2021)
                     /locus_tag="VI359_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY6972721.1"
                     /translation="MANLSAPNLHQEVGMYDVKNLDKMKTLGVHAPEAMKAFVAFDRA
                     ALAEGAIPRKYKELIALAVAFTTQCPYCIHLHADKAREMGASDPEIAEAVLVAAALRA
                     GGAITHGTHAMKGA"
     gene            complement(2043..2864)
                     /locus_tag="VI359_00315"
     CDS             complement(2043..2864)
                     /locus_tag="VI359_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6972722.1"
                     /translation="MSTSTLAPEVESLKTRLKHIWMAGDYDRFSRYMEGDAREFYQRL
                     HVVPGSRLLDVGCGSGQLALIAAKDGLEVTGVDIASNLVERARVRAQAEGLQARFEEA
                     DAEALPFEDASFDVVASLIGAMFAPRPDLVAKELLRVCKPGGTIAMANWTPQGFIGQM
                     FKTVSKFIAPSGMPSPVLWGDETTVRERLGKGLSELRLVRRHYRFSYPFPPSEVVEFF
                     RLYYGPTNQAFASLDVGGSESLRLELEALWSSHNRAGSDCTTVFGEYLEVIGIRA"
     gene            3068..>3934
                     /locus_tag="VI359_00320"
     CDS             3068..>3934
                     /locus_tag="VI359_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY6972723.1"
                     /translation="MDVLSEVLKAVRLDGAMFFNAEFSAPWCFRSPASRTVAPYLSAD
                     ARHVIIFHLLTDGRGYAHVEGDGRSVPLDAGDIVIFPHGDPHIMGNGSPVKPVDNEQE
                     LQRVLSQGLKVSRLGGGGEITKFICGYMTCDPHLSRTLLGGLPPILKINIRNDASGQW
                     LENSLRYSVDNADASRPGGEAVLAKLSEVLFVETLRRYMALLPQEQTGWLAGVRDSEV
                     GKALALLHRKPAHPWTIATIANEVGISRSVLAERFRRYLTETPIAYLTRWRLQLGAQM
                     LKSTSSGVAQIAA"
BASE COUNT          821 a         1187 c         1064 g          862 t
ORIGIN      
        1 ggcttgaaag acgggagtgc cgccttcggt cgtcacccca gtatagggac gatcgggatt
       61 cacgaggatc tccgtcagac cggtttgctc gaattcggtg gttggcctat tctttttgtt
      121 tatcgcccaa ttgctaatca accggaatcg taacccactt cgctctgcag ctacgagctg
      181 ccccgcttcg attaagaact gggctggcag agggagtcgg gccgtctctc tccaattttc
      241 cgaggcgacg acaatgccgc gcatctgcat ccgctccaca atgtcagtgg tgtaaaacgt
      301 tcgatctgcc tggatcacac tatgcacgta gttggcgaca gattcggccg gtatacactt
      361 gggcgacgac gcctccctga ttgcggcaga gacaacccac tgaccgaata gacaagttga
      421 gattattcca accgcgagtc ctatccaaaa gcctctcacg ttcatgatgc gatctccatt
      481 tctttttctg cttatcagat tcactccgtg gcaccgctgg caaatctctc aaaccaagat
      541 ggtctcatcg ttctagaacg aattatcatt gaactaggaa aaagcatacg ccgatgaacc
      601 tgataaatcg agatgagccg tcagggattt cagctccgat tgtgaatgcg aggtagtgaa
      661 agggtggaat gaaccgtgtc gcggcgggtg cggcagtact ctcgatgtgc tgctcctacg
      721 cggtcggagc ggtctgtcgt gtccttcacc gaaatgattg attgagatgc acaatcgcga
      781 ccgatagcgc atgatcgagg gcggagcgca gcgcgggctg caattcgtaa tcgctcgcat
      841 agtcgctcca cgcgccggag tggggaaccg gcatggcggt cacgatgtcg tggtacagca
      901 cctcaccact ctctaccgat ataatcttgc ttgatacgac gaagggcggc atcttcccgt
      961 aaaaacgagc cagcaaccgt tctctccaag tcggcatatc aatccgaaaa agaataatgc
     1021 tatccgcccc gagccacttc ccgacccgca ctgcgctgtc gtctccaaca cgcccagaga
     1081 gctgtaatcg ctgctcatcc gtcaacggcc cgatgttgcg ccgctcgaca atcttgatcc
     1141 acgaccgctg cgtcttgagc tggaaggccg cttgctcaag cctgctgtac ccatacgcca
     1201 cgtcctgttc cgacgtgttg ggataccaga cggctaagcg ccggacatgc tgggggactg
     1261 gaaacccgat tatcgccaga tttgctgggg cttgatcgac ggcgatgggt ggcgtaggcc
     1321 gacaggccgt gtccatccat agactgacca tcgcggcagt ggcggcgagg aacgccgacc
     1381 gctcgatgcg aggtctggca tccatagcgc cagggtacga gcagacagcg ggctcgcgct
     1441 atcctacgga caggggaaaa ctgctacgga tagggggagt gtgacgcgtg gcggacctaa
     1501 tccttgggct ctggtcactc aacgcgtgga tacggaactt ccgccatcgg cctctcaccc
     1561 cttacccttt accccttact gtcccacccc gtcctgtcac gccgtgatgg accggcctgc
     1621 cccttaacga gctgaatgcc cagatttctg agcccagtcg agaatgtcgt tcaggctccc
     1681 ttcatggcgt gtgtgccatg ggtgatcgcc ccacccgcgc gtaacgccgc agcaacgagc
     1741 acggcttcgg cgatctccgg atccgatgca cccatttctc gcgccttgtc cgcgtggagg
     1801 tggatgcagt aagggcattg tgtcgtgaac gcaacggcaa gcgctattag ctccttatac
     1861 tttcgcggaa tcgccccctc agccaaggct gctctatcga aagccacaaa cgctttcatc
     1921 gcctcgggtg catgaacccc gagagttttc atcttgtcaa gattcttcac atcgtacatg
     1981 cccacctcct gatgaaggtt gggtgctgaa agatttgcca tgctcttcct cctttggttg
     2041 cttcacgcac gaatgccgat cacttccaga tattcaccga agaccgtggt gcagtcactc
     2101 cccgctcgat tgtgcgaaga ccagagtgct tcgagttcca ggcgaagact ctcactgccg
     2161 ccgacgtcaa gggaggcaaa cgcctgattc gttggtccat agtagaggcg aaagaactcc
     2221 accacctcgg agggaggaaa gggatagctg aatctgtaat gacgccttac gagacgtagc
     2281 tccgacagac ccttgccgag ccgttcacgg accgtggttt cgtcgcccca caggaccggc
     2341 gaaggcatcc cggagggcgc aatgaatttc gagacagtct tgaacatctg cccgatgaaa
     2401 ccttgtggcg tccaattggc catggcaatg gttccccccg gcttgcacac tcgcagcagt
     2461 tccttcgcaa ccagatccgg tcgtggcgcg aacatcgctc cgattaagct cgccaccacg
     2521 tcaaagcttg catcctcgaa ggggagcgcc tccgcatcgg cttcctcgaa gcgggcttgc
     2581 aaaccctccg cttgtgcgcg cacccgtgcc cgttccacca aattactcgc gatgtccaca
     2641 ccagtcacct ccaagccatc cttcgcagca attagcgcca actggccgga gccacagccc
     2701 acatccaaca atcggcttcc gggaacgaca tggagtcgct ggtagaactc tcgcgcatcg
     2761 ccttccatat accgcgagaa tcgatcgtag tcgccggcca tccagatatg cttgagacga
     2821 gtcttcaaac tctccacttc tggtgctagt gtactggtcg acatggtttc ctcctttgca
     2881 tgtgctcgga tcgaatgacg gttgagcatc gccgttccaa cgcctagtca cactatagga
     2941 tgattattta caggggtctt atccggaagt ctggttctct tacctggggc gtgctaggct
     3001 gcgttttcct tgcttaggcg tccggcacag tttgccgaaa cgccggaact gcaagtgtgg
     3061 agtctgtatg gatgtcctgt ccgaggtcct gaaggccgtc aggctcgacg gagcaatgtt
     3121 ttttaatgct gagttttcag cgccctggtg ctttcgttcg cccgcctcgc gaaccgtcgc
     3181 tccgtatctt tcggcagacg cgagacacgt gatcatcttt cacctgctaa ccgatggccg
     3241 cggctatgcc catgtcgaag gggacggtcg gtcggttcct ctcgacgccg gggacatcgt
     3301 catctttccg cacggcgatc cacacatcat gggaaacggt tcacccgtca agccggtgga
     3361 caacgaacaa gagctgcaac gagttttatc tcaaggcctc aaggtctccc gattgggcgg
     3421 aggtggagag atcaccaaat tcatctgcgg ctacatgacc tgcgatccgc atctgagccg
     3481 gaccttgctt ggcgggttgc cacccattct gaagatcaat atccgcaacg acgcatcagg
     3541 ccagtggctc gaaaactctc tccgctactc ggtggacaat gcggatgcat ccaggcccgg
     3601 tggcgaggcc gtactcgcca agttgtccga agtgttgttc gtcgaaacac tccgacgcta
     3661 tatggcactg ctcccgcagg aacaaaccgg gtggctggcc ggcgtccgcg actcggaagt
     3721 gggaaaggcg ttggctctgc tgcatcgcaa gccagcccat ccttggacga ttgccacaat
     3781 cgcgaacgag gttggaattt ctcgttccgt actggccgaa cgtttccggc ggtatctcac
     3841 ggaaactccc atcgcctacc tcacacggtg gcggcttcag ctcggcgcgc aaatgctcaa
     3901 atccacgagc agtggcgttg cacagatcgc tgct
//
LOCUS       DATDNW010000014         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_412166, whole genome shotgun sequence.
ACCESSION   DATDNW010000014 DATDNW010000000
VERSION     DATDNW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412166"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..141
                     /locus_tag="VI359_00325"
     CDS             7..141
                     /locus_tag="VI359_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6972724.1"
                     /translation="MRSFTLPDDADGTKVNAEYKDGVLKVHLPKSEKAKPKAIEVKIA
                     "
     gene            263..418
                     /locus_tag="VI359_00330"
     CDS             263..418
                     /locus_tag="VI359_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972725.1"
                     /translation="MFRHPNYLEVPWDIHWHPEATRHHRHLWWLIGALVLLAMFLIGV
                     GVTSGLM"
     gene            454..1125
                     /locus_tag="VI359_00335"
     CDS             454..1125
                     /locus_tag="VI359_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY6972726.1"
                     /translation="MVFQNREEAGRRLAAELMPYREDPDGVILALPRGGVAVGYQLSL
                     ALHLPLDVFITRKLGAPGNQEYALGAVGETGTVYLNPTAVAEFRLSRADVQELVKAQQ
                     KEIARRQKLYRQGRRMPTVTDRTVILVDDGIATGSTFFASVEAIKHLKPKRLIGAIPV
                     GPVDTIAAARKQVDELVVLATPIPFWAVGNHYVDFTQVEDRDVVEYLNLAEEGLRERT
                     HSSPA"
     gene            1143..1643
                     /locus_tag="VI359_00340"
     CDS             1143..1643
                     /locus_tag="VI359_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEY6972727.1"
                     /translation="MASQGKIPAGLGARRDRTVQEYQHDTYKLRGKLKEPTVCPDCGA
                     VFHKGHWMWGPTPKGAEQATCPACHRIRDKYPKGFVTIKGPFKDEQHEQFIGIVKNTE
                     EKEKKEHPLSRIMGIESQREGLVISTTDTHLPRRIGEALKHAYHGELELHYDQDEEFV
                     RVTWTH"
     gene            1789..2337
                     /locus_tag="VI359_00345"
     CDS             1789..2337
                     /locus_tag="VI359_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6972728.1"
                     /translation="MKVPGRRCLRTITFLMLVIASLFGLSAAQAKIVVPANAVIEADD
                     ATVSSVVAMFERAEQAVKAHDLETVLNLYSSKYNYHGLKKDDIRKIWKDLFDEYSDIA
                     STHLFSKIAKVGSGTQAVVEVTCTGHLWAVSKTSKLYIPIDSWHEEVHFLIFEDGAWR
                     IRGNVGESPRVLPFGTAPHPLF"
     gene            2362..>2766
                     /locus_tag="VI359_00350"
     CDS             2362..>2766
                     /locus_tag="VI359_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6972729.1"
                     /translation="MRIAIGVDWSDQAFTAVTQTFHAYHPSDVTLVHGVDLGMFEYPI
                     VAEAANLQGYDDFRNAMVDAGRQLVDRAAAMIPDDVGNVRRINEIGSPAQVILNVAKT
                     VAADLIVVGARGRSRVSELVLGSVSHRVLSHAP"
BASE COUNT          644 a          777 c          809 g          536 t
ORIGIN      
        1 agctttatgc ggagcttcac cctgccagat gacgccgacg ggacaaaggt caacgccgag
       61 tacaaagatg gcgtcctcaa ggtgcacttg ccgaaatcgg aaaaggcgaa accaaaggcc
      121 attgaagtca agatcgcgta acgccaaact agtggagacc gagtccaaaa tgtgcaggct
      181 gccgtggagc ggttgcgaaa gatggttgag tactcttagc gcctcaccgg tttcccttat
      241 ggggatctaa aaggaggttg ttatgtttcg tcaccccaat tatcttgagg tgccctggga
      301 cattcactgg catcccgaag caacgaggca tcaccgccac ttgtggtggc tgatcggagc
      361 gttggtcctg ttggctatgt tcttgatcgg agtcggcgtc acgagtggac tcatgtagtc
      421 gctgacgtgc cgggcagtgg gaaaggagtg tcagtggttt ttcaaaatcg cgaagaggcc
      481 ggacgccgtc tggccgccga actgatgccc tatcgggaag atccggacgg cgtgatcctg
      541 gcgctccctc gaggcggagt cgccgtcggc tatcaactca gtctggcgct gcatctcccg
      601 ctcgacgtct tcatcactcg gaagttgggc gctcccggca atcaggaata tgcactcggt
      661 gcggtcggtg aaacagggac ggtctatctg aaccccacgg cggtcgccga gtttcgctta
      721 tcacgggcag acgttcagga actggtaaaa gcgcaacaga aagaaatcgc ccgccgccag
      781 aaactctacc gccagggacg ccgaatgccg acggtgacgg atcggactgt aattctggtc
      841 gatgacggaa tcgccaccgg gtccaccttc tttgcatcgg tggaagcgat caaacacctc
      901 aagccaaaac gcctgatcgg agctattcca gttgggccag tggatacgat tgccgccgca
      961 cggaagcaag tcgatgaatt ggttgtgctt gctacaccga tcccattctg ggcggtgggt
     1021 aaccattacg tggacttcac gcaggtcgaa gaccgcgatg tggtggaata cttgaacctg
     1081 gccgaagaag gattgcgaga gcgaacgcat tcatctccag cctaaacgag caaggagtac
     1141 tcatggcatc acaaggcaaa ataccggcag gtctgggcgc acggcgagac cgcaccgtcc
     1201 aggaatatca gcacgacacg tataaactcc gcggcaaact gaaagaaccc accgtctgtc
     1261 ccgattgcgg cgcggttttc cacaagggac actggatgtg ggggcccacg ccaaaggggg
     1321 cagagcaggc cacctgcccg gcctgccatc gcatacgtga caagtacccc aagggcttcg
     1381 tgaccatcaa aggacctttc aaggatgagc agcacgagca gttcatcggc attgtgaaaa
     1441 acacggagga gaaagagaag aaggagcacc cgctctcacg aatcatgggc attgaatccc
     1501 agcgcgaggg cctggtgatt tccacgaccg atacccatct gcctcgacgg atcggtgagg
     1561 cactgaaaca tgcctatcac ggcgaactag agttgcatta cgaccaggat gaagagttcg
     1621 tgcgagtcac ctggacgcac taatcgtcag cggtcagcga cgaacttcgg agccggagtt
     1681 caccggaggt gtcgtgcgaa gtcgtcgttc ggacgttgaa aaggatttgt gccgatagta
     1741 cctcggtcgg actatgattc gataacaagg ggaggagacc agaagaccat gaaagtacca
     1801 ggccgcaggt gcctccggac tatcaccttc ctcatgctcg taatcgcctc cctcttcggg
     1861 ttgagtgcag cacaggccaa gatcgtcgtc ccggccaatg ccgtgatcga ggcggacgac
     1921 gcgacagtca gcagcgtcgt cgcgatgttc gaacgggctg aacaggccgt gaaggctcac
     1981 gatctcgaga cggtcctgaa cctctactcc tccaagtaca actatcacgg cctcaaaaaa
     2041 gatgacattc ggaaaatctg gaaagatctt ttcgacgaat atagcgacat cgcgagcacc
     2101 cacctcttca gcaaaatcgc gaaggtcgga tcgggaaccc aggccgttgt ggaagtcacg
     2161 tgcaccggtc atctgtgggc agtgtccaaa accagtaagc tctacatccc gattgacagc
     2221 tggcatgaag aagtgcactt tctgatcttc gaggacgggg cctggcgcat tcgcggcaat
     2281 gtcggagaat cgccgcgcgt gctgcccttc ggcaccgctc cccatccgct cttttgaccg
     2341 cgcagcggag gagtacaccg gatgagaatc gctatcggcg tcgattggtc cgatcaagcc
     2401 ttcacggctg tgacgcaaac gtttcacgcc taccatccga gtgatgtgac tcttgttcat
     2461 ggcgtcgatc ttggcatgtt tgaatatccg atcgtcgccg aagccgctaa cttgcaggga
     2521 tacgacgatt tccgcaatgc gatggtcgat gcggggcgcc aactcgtcga tcgtgcggca
     2581 gccatgatcc cagacgacgt cggcaacgtg agacggatta atgagatcgg cagccccgct
     2641 caggtcatcc tcaacgtcgc gaaaaccgtc gccgcagatc ttatcgtggt gggcgcgcgc
     2701 gggcgcagcc gtgtcagcga actcgtgctc ggcagtgtct cccatcgcgt gctctcgcac
     2761 gccccc
//
LOCUS       DATDNW010000015        10204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1299464, whole genome shotgun sequence.
ACCESSION   DATDNW010000015 DATDNW010000000
VERSION     DATDNW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299464"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VI359_00355"
     CDS             complement(<1..346)
                     /locus_tag="VI359_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6972730.1"
                     /translation="MQKITPCLWFNDQAEEAMRFYVSVFKNSKVGRVTRYGEAGAKVS
                     GRPKGSVMTATFEIEGQEFMALNGGPHFTLSEAVSFIVKCDMQKEIDTFWEKLSEGGE
                     KGVCGWLKDKFGL"
     gene            complement(402..758)
                     /locus_tag="VI359_00360"
     CDS             complement(402..758)
                     /locus_tag="VI359_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6972731.1"
                     /translation="MKYMLLIYFEEKKGLSEAERQECYAESTALAHQLHANRQFVAAD
                     PLHPTSMATSVKVRDGKRLVTDGPFAETREQLGGYVLIDAKNLDEAIAVAAKIPMARK
                     GTVEVRPVIDLPGLPS"
     gene            complement(913..1704)
                     /locus_tag="VI359_00365"
     CDS             complement(913..1704)
                     /locus_tag="VI359_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6972732.1"
                     /translation="MTKSTIEHPIVVSRAEWLAARKALLAKEKELTRFRDALNAERRR
                     LPMVKIEENYVFDTPGGKKSLGELFADNSQLMVYHFMFGPDWEEGCRSCSLVSDHIDG
                     ANWHLPQRDVRLLAVSRAPLAKIEKFKNRMGWRFEWVSSLGSDFNSDFRVSFTKDEMA
                     QGKVNYNYTMQEFPSEEAPGISVFYKDTGGNVFHTYSAYARGLDMLVGAYNFLDLAPK
                     GRQEDWEQPPGRSDGPFMHWVRHHDRYEAVAKAPASCCSSGKDHS"
     gene            complement(1864..2241)
                     /locus_tag="VI359_00370"
     CDS             complement(1864..2241)
                     /locus_tag="VI359_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6972733.1"
                     /translation="MKYLLFCCHEEKKMDAMSKSECDAIMDETSAYCDALQKSGHLIA
                     AEPLESVRTAVTVRVRTGKLSVTDGPFAETKEQVAGFFLINARDLNEAIQVASKFPSV
                     RFGSIEVRPVMELHGCSRPAADS"
     gene            complement(2389..3651)
                     /locus_tag="VI359_00375"
     CDS             complement(2389..3651)
                     /locus_tag="VI359_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6972734.1"
                     /translation="MNDDATKRVRELVEDVYRSESRQVLATLIRLLGDFDTAEEALHD
                     AFAVAVEQWARDGVPANPRAWLVSTGRFKAIDGMRRRARYDESLTELAKELETTTTDV
                     EEYDDDSVEDDRLRLIFTCCHPALPSEAQVAMTLREVCGLATEEIARAFLTKPATIAQ
                     RIVRAKAKIRDARIPYEVPSERDLPDRLEAVLRVVYLVFNEGYSASTGASLTRHDLSA
                     EAIRLGRLLIELLPEPEAVGLLALMLLHDSRRSARTSPSGDLILLEDQDRALWNKNQI
                     TEGISLVDRALRSGPVGPFAIQAAIGAVHAQAPNAAATDWAQIVALYDVLLRAEPSPV
                     IELNRAVAVAMRDGPSAGLALIDALLARGDLDNYHLAHAAHADLCRRLGRTERARASY
                     ERALTLTQQEPERRFLTRRLSELSDRTS"
     gene            complement(3882..4475)
                     /locus_tag="VI359_00380"
     CDS             complement(3882..4475)
                     /locus_tag="VI359_00380"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6972735.1"
                     /translation="MKDSARVTEVAPDHYAISVYVPEFNLRFNHFLIKDEEPLLFHTG
                     MKQMFPLVRDAVACVINPSTLRWISFSHFEADECGALNEWLHLAPHAQPACGLVGALV
                     SVNDFSLRRARVLAQDETLITGKYRFRFRQTPHVPHNWEAGLLFEEVTRTLLCSDLFS
                     HEGDVEPVTKSDVVDRARQALIEGQKDHLPTPIPTRR"
     gene            complement(4579..5067)
                     /locus_tag="VI359_00385"
     CDS             complement(4579..5067)
                     /locus_tag="VI359_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6972736.1"
                     /translation="MSYPDVRYFGDKGEISATFRSADQKPELSIGSGTVVRYLATGAS
                     THGQFGLYRWDARPHTPGPSAHFHRTMSESFFILSGTVRLFNGERWIDATAGDFLYVP
                     EGGLHGFHNESSEPASMLLLFAPGASREGYFEALAQKAAGRQFSDEEWRELCLGHDNH
                     FV"
     gene            complement(5079..5543)
                     /locus_tag="VI359_00390"
     CDS             complement(5079..5543)
                     /locus_tag="VI359_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY6972737.1"
                     /translation="MKDDLERVKLLARDLRNGKEFPRSPRETLGGYVLAARALDKCRA
                     VLAGWQGEYHSNCPLDQRWLKFAEIEYNAYRSFVATGATDEEVAVWIGEHARKRSRVD
                     IVVWNNKERDLRLSDLSPELQEYMEGYIASSVPRHRVVYRWFDVYDLEEQRL"
     gene            complement(5561..6298)
                     /locus_tag="VI359_00395"
     CDS             complement(5561..6298)
                     /locus_tag="VI359_00395"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY6972738.1"
                     /translation="MSRLKEKVAIVTGASDGIGRAIAERLAQDGATVVVNYGKSADKA
                     KQVVAGIEAKGGKAVAIQADMSKVGDAGRLVKDTVKKFGRLDILVNNAGMFMFKPLVD
                     MSEEEFDRMFALNTKGPYFALQEAAKVIQDGGRIVNISTDGTHIGFGGATAYLGSKGA
                     LEQFTKGLAHELAPKGVTVNTVSPGYTDTAMMPADPAFRQMGEQASPLKRLGLPKDIA
                     DVVAFVVSEEARWLTGQNIHTGGGVVM"
     gene            complement(6355..6969)
                     /locus_tag="VI359_00400"
     CDS             complement(6355..6969)
                     /locus_tag="VI359_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972739.1"
                     /translation="MERTLKRMNVATLMAMAVTVGGILLCLHLLFLPDTIGATVPEAK
                     ANGSVDLEVSLRWIQPILGQAIVEDTLIKQRYGSEIGEAINVPNEATAASYRLHGTNP
                     YAQASAYAGAMVVDHATRTQWVMGRLIAELTRHRVRAGTLTADRLAEEENQRIITIAQ
                     QAGKKSDDVFLTELQTRLGQVIVSDTVNQVHTAERNPDRVGLIF"
     gene            complement(7019..7975)
                     /locus_tag="VI359_00405"
     CDS             complement(7019..7975)
                     /locus_tag="VI359_00405"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY6972740.1"
                     /translation="MNEAIEFLAQHGTLVLFVTVFAEQVGLPLPAVPFLIAAGALVGT
                     GQMALGMAVGSAVLAALAGDQVWFELGRRRGRRVLNWLCRISLEPTSCVRRTEDLFAR
                     HGVRSLVVAKFVPGLSTIAPPLAGIVGLPVPQYLFYNGLGAILWVGSGIGLGYLFSDE
                     LEHALALSAQVGPTVMLILLGGVTGYVVLKALRRRQAESLVPRLTAQEVADKLATGED
                     PIIIDLRSHGARQEEPGISGSLSLSLEDLKAKHQDLPRDRDVILYCDCPEDAASVQGT
                     ALLRKLGFTRVWALAGGIEAWRAITEKAEDHLRVAAGETVAV"
     gene            complement(8192..>10204)
                     /locus_tag="VI359_00410"
     CDS             complement(8192..>10204)
                     /locus_tag="VI359_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY6972741.1"
                     /translation="ERYQALLEVAEAVSAHRDLHELFRDLAQRLPRVVQVNFVSLSLH
                     DPTRNIMRLHTIQANVPADLIGGHEEPVEETPAGLVWQTQQPILVPDLAEEHRWPKVT
                     QRMQEDGTKSFCVVPLTTAVRRLGAMGFSSLERGVYGEADLEFLRQVGNQVAVAVDNV
                     LHHQDLTHDRDRLRLLLEVSESIASHRDLAELFHDLAQRLPRALEFSYIALILHDPER
                     NVMRLHILETPRPVSIAPGCEFPVEESVGGWVWQHQQPLVIENIEQETRFPKLIQMIC
                     EAGVRSFCTVPLTTARRRLGALSVGSFEKHHYGSASIEFLQQVAKQVAIAVENALAYG
                     EISGLKEKLAKEKLYLEEEIRTEYNFDEIIGESPTLKKVLKQVEVVAPTDSAVLVLGE
                     TGTGKELLARAIHNLSGRRERTFVKMNCAAIPTGLLESELFGHERGAFTGAIATKVGR
                     FELADGGTLFLDEVGDIPLEIQSKLLRVLQEQEFERLGSTRTMRVNVRVVAATNQDLS
                     EMVEKKEFRSDLYYRLNVFPLMVPPLRERREDIPVLVRYFAQKFARNLNRSIERIPAD
                     TMDALAAYAWPGNIRELENLVERAVILSTGPDLVVPVSELKAPRSQSGAASVSTLEAA
                     EREHIVRALNATNWVIGGQAGAAAKLGMKRTTLQSKMQKLGISRQK"
BASE COUNT         2034 a         3154 c         2850 g         2166 t
ORIGIN      
        1 aaagcccgaa cttgtctttc agccagccgc agaccccttt ctctcctccc tccgagagct
       61 tctcccagaa cgtatcgatt tctttctgca tgtcgcattt cacaatgaac gagacggcct
      121 cactcaatgt gaagtgagga cctccattga gcgccataaa ttcctgccct tcgatctcaa
      181 atgtcgccgt catgaccgac cctttgggcc tcccggacac cttcgctccc gcttctccgt
      241 agcgggtcac gcgaccaacc ttcgagttct taaagaccga cacataaaac ctcatcgcct
      301 cttctgcctg gtcgttgaac cacaagcatg gtgtgatctt ttgcatcacc gcgtactcct
      361 ttgcatgacc ttactcgtgc gagcagcctg acgttctccg ctcaactcgg caaccccggg
      421 aggtcgatca ccggccgaac ttcgacggtg cccttgcgcg ccatgggaat tttcgcagcg
      481 acggcgatgg cctcatcaag attcttggca tcgatgagga cgtagccgcc cagttgctcg
      541 cgcgtctccg caaacggacc gtcggtcacg aggcgtttgc cgtcgcgcac ttttacgctg
      601 gtcgccatcg acgtcgggtg gagcgggtca gcggcgacaa actgtctgtt tgcatgcaac
      661 tggtgcgcga gggcagtgga ctccgcataa cactcttggc gttcggcctc gctcaacccc
      721 ttcttttcct caaagtagat tagcaacatg tatttcatgt ggaggcctcc atcagtcagc
      781 gaaatggtga gtgcccggat cgaaactcca atggcaactc tttcatcatg gtgcaatgct
      841 cttgatcact atgcaactgt cgccttgggc tatgggccgt tgagttcttg acttcgccgc
      901 taccctacgt gctcacgagt gatccttacc tgagctacaa caggatgccg gggcctttgc
      961 aacagcctca tatcgatcgt ggtggcggac ccagtgcatg aacgggccgt cgcttcggcc
     1021 cggtggctgc tcccagtcct cctgtcggcc cttcggcgcg agatcgagga agttatacgc
     1081 gccgaccagc atatcgagcc cgcgcgcgta ggcggagtag gtgtggaaga cgttgccgcc
     1141 ggtgtccttg tagaagacgc tgatgcccgg cgcctcctcg ctgggaaact cctgcatcgt
     1201 atagttgtag ttcactttgc cctgcgccat ctcatccttt gtgaacgaca cgcggaagtc
     1261 agagttgaag tcgctgccga gggaagacac ccactcgaag cgccagccca tgcgattctt
     1321 gaatttctcg atcttggcta gaggcgcgcg agagacggcc aacaggcgca catcgcgttg
     1381 cggaagatgc cagttggccc cgtcgatatg atctgacaca agcgagcagc tgcggcaacc
     1441 ctcctcccag tccggtccaa acatgaagtg atagaccatg agctgactat tgtccgcgaa
     1501 gagctcgccc aaagacttct tcccgcccgg cgtgtcgaag acgtagtttt cttcgatctt
     1561 gaccatcggt agcctgcgcc gctcggcgtt cagtgcgtcc cggaagcgtg taagttcctt
     1621 ctccttagca agaagtgcct tgcgcgcagc cagccactca gctcgcgata cgactatcgg
     1681 atgttctatc gtgctcttgg tcatgatcca gcctcctttg atgggaattg tgtttggttt
     1741 ctcgtcgtcc ctgtcatttt acgaagcttg tttaccgcaa gtgccgtcag tccgcctgtc
     1801 gaggtcgtcc cagctgcaat cagtgccaga gttgtgaggc atgccggaca catgagtgac
     1861 tcctcacgag tcagccgccg ggcggctaca gccgtgtagc tccatgaccg gccgcacttc
     1921 aatgctcccg aaccgcactg atggaaactt ggaggccacc tgaatggcct cgttgaggtc
     1981 tcgggcgttg ataaggaaaa accccgcgac ctgctccttc gtttctgcga aaggaccgtc
     2041 ggtcacggac agcttgccgg ttcggactcg cacggtcacg gccgtccgaa ctgattcgag
     2101 gggctccgcc gcaatgaggt gaccactctt ctggagcgcg tcgcagtagg cggaggtctc
     2161 atccatgatg gcatcgcact cgctcttgga catggcgtcc atcttcttct cctcgtggca
     2221 acagaagagc agatatttca tgactcccac cctccaattg gatgtgaagc gttgtttcta
     2281 tgagatagtc gttcgggaag aagaaaatcg acatgagacc ggctgggtcc accagatcga
     2341 gagcagttct tggcgaatca acgacttaga tcgagcggcg ggaggcgatc aactggtccg
     2401 atccgacagc tcgctcagcc ggcgcgtgag aaaccgacgc tcaggttcct gttgggtgag
     2461 ggtgagggct cgttcgtagg aggctcgggc gcgttccgtt cgcccgagtc gccggcagag
     2521 gtccgcgtgc gccgcgtgcg ccaagtgata gttatccaaa tcgccccgcg cgaggagagc
     2581 atcgattaag gcgagaccgg ccgaagggcc gtcgcgcatc gccacggcga ccgcccgatt
     2641 caactcgatc acaggcgagg gctcggcccg caagagcacg tcgtagagcg cgacgatctg
     2701 agcccagtcc gtcgcagcgg cgttgggcgc ctgcgcatgc acggctccga tcgccgcttg
     2761 gatggcgaac ggcccgaccg gaccggaacg caacgcccgg tccaccaacg agatgccctc
     2821 tgtgatctga ttcttgttcc acagggcgcg atcttgatcc tcgagaagaa tgagatctcc
     2881 gctcggcgac gtgcgcgcgg agcgccgcga gtcgtgcaga agcatcagcg ccaggagtcc
     2941 gaccgcttcc ggctctggaa gtaattcgat caacaaccgc cccaggcgga tggcttcagc
     3001 cgagagatcg tgccgcgtga gcgacgctcc ggtggaggca gagtagccct cgttgaatac
     3061 gagatagacg acacgaagca ctgcctccag ccgatccggc aggtcccgct ccgacggaac
     3121 ctcgtaggga atgcgcgcat cacggatctt cgctttggcg cggacgatcc gctgggcgat
     3181 cgtagccggc ttcgtgagga aggcgcgcgc aatttcctca gtagcaaggc cacacacttc
     3241 acggagcgtc atcgccactt gggcttcaga cggcaaggcg ggatggcaac aggtgaagat
     3301 taatcgcagc cggtcatcct cgacactatc gtcgtcatat tcttcgacgt cagtggtcgt
     3361 ggtttccagt tccttggcga gctccgtcag cgactcatcg tatcgagcac gtcggcgcat
     3421 gccatcgatg gccttgaaac ggccggtaga cacaagccat gcgcgcggat tggccgggac
     3481 accatcccgc gcccattgct cgaccgccac agcgaacgca tcgtgcagcg cctcttcggc
     3541 ggtgtcaaag tcgccaagta gacggatcaa ggtcgcaagg acttgccgcg attcggagcg
     3601 gtagacgtct tccaccagct cgcgcacccg tttggttgcg tcgtcattca ctgtcgacaa
     3661 gctctcatac tgagatggtc cagaaggacc aacgtggtca gcgccacgcg cgggcaagtt
     3721 gaatggttcc taacaccttc ctcatccgcg ccccagaacc tcccgcatgg tagtcgccaa
     3781 gtcgcgcaac gcccgttcgc cgtcatctat gaacgaagac ccgtgcatga gcgccaactg
     3841 tttcggccga agatcggcca ggccatgtag gataggctcg gtcaacggcg tgtagggata
     3901 ggcgttggca aatgatcttt ctgcccttcg atcaacgcct gcctggcgcg atcgaccaca
     3961 tccgacttgg tgaccggctc gacgtctcct tcgtgagaga acagatcgga gcaaagcaac
     4021 gtccgcgtga cttcttcgaa cagcaggccc gcctcccagt tatggggcac atgtggcgtc
     4081 tggcgaaacc gaaaacggta ctttccggtt atgagtgtct catcctgcgc gagcacgcgc
     4141 gcccggcgaa gtgaaaagtc gttcacgctc accagcgccc cgaccagacc gcacgccggc
     4201 tgcgcgtgag gcgctaggtg gagccattcg ttcagcgctc cacattcgtc agcctcgaag
     4261 tggctgaatg atatccatcg caacgtagaa ggattgatga cgcacgcgac tgcatcccgc
     4321 accagcggga acatttgttt catccccgtg tgaaacagga gcggctcttc gtccttgatg
     4381 aggaaatggt tgaaccgaag attgaattcc gggacgtaga ccgaaatcgc gtagtgatcg
     4441 ggcgcaacct ccgtcactct ggccgagtct ttcattgcgt ctcctctcac gatcactcga
     4501 aaaagaactc tgcgacacct ttcgcgctgt gagcggtgag acagaccctc gagagccctt
     4561 atgattcagg accagcggtc acacgaagtg gttgtcgtgg cccaggcaga gctctctcca
     4621 ttcctcgtcg ctaaactgac ggcctgcggc tttctgggcg agtgcttcga agtatccctc
     4681 gcgcgatgca ccaggcgcga agagaagtag catcgaagcc ggctcgctgg attcattgtg
     4741 gaagccatgc agccctccct cggggacgta caaaaaatcc cctgcagtcg cgtcgatcca
     4801 gcgctctccg ttgaagagac gtaccgtccc tgacagaatg aaaaacgatt ccgacatcgt
     4861 tctgtggaag tgcgcgctcg gtcccggcgt gtgcggtctc gcgtcccacc tgtacaagcc
     4921 gaattgtccg tgtgtggacg ctccggtagc caggtaacgc accactgtgc cggagccaat
     4981 cgacaactcc ggcttctgat ccgccgaacg aaacgttgca ctgatttcac ccttgtcacc
     5041 gaaataacgc acatccgggt atgacatcca tgactccttc agagacgttg ttcttccaag
     5101 tcgtacacat cgaaccagcg atacacgacg cggtggcgcg gaacggaaga ggcaatgtag
     5161 ccctccatgt attcctggag ttcgggtgac agatcgctca agcgcaggtc acgttccttg
     5221 ttgttccaaa cgacgatgtc gacacgggaa cgtttcctgg catgttcgcc aatccacacg
     5281 gcgacctctt catcggtcgc gccggtggcg acaaacgatc tgtacgcgtt gtactcgatt
     5341 tcggcgaatt tcagccaccg ttgatcgagc ggacagttgg agtggtattc gccctgccag
     5401 ccagcaagga cggctcggca tttgtccagc gcacgagcgg cgagcacgta gccccccagc
     5461 gtttctcgag gactgcgcgg aaactctttg ccgttccgca aatcacgggc caacagcttc
     5521 actctttcaa gatcgtcttt catgccgttc tccttactga ttacatcacg acgccgccgc
     5581 cggtgtgaat attttgtccc gtcaaccacc gcgcctcttc gctgaccacg aaggccacga
     5641 cgtcggcaat gtctttgggc aggccgagtc gcttgagcgg ggaggcttgc tcgcccatct
     5701 gccggaaggc aggatcggcg ggcatcatgg ccgtgtctgt gtagccgggg gacaccgtat
     5761 tcaccgtgac ccccttgggg gccagctcgt gggccaggcc tttcgtgaac tgttcgagcg
     5821 cgcccttgct gccgagatat gctgtggcgc cgccaaaccc gatatgggta ccgtccgtgg
     5881 aaatgttcac gatgcgtccg ccgtcctgga tgactttcgc cgcttcctga agcgcaaagt
     5941 acgggccttt agtattgagg gcaaacatgc ggtcgaactc ttcttcgctc atgtcgacca
     6001 gcggcttgaa catgaacata cccgcgttat tcaccagaat gtccagccgg ccgaatttct
     6061 tcaccgtgtc cttcaccaac cggcctgcat cgccaacctt gctcatgtcc gcctggatcg
     6121 cgacagcctt gccgcctttc gcttcgatgc cggcgacgac ctgcttggcc ttgtccgcac
     6181 tcttgccgta attcacgacc actgtggcgc cgtcctgcgc caaccgctct gcaatcgcac
     6241 gaccgatgcc gtctgatgcc ccggtcacga tcgctacttt ttctttgagc ctgctcatgg
     6301 ttctgtctcc ttcctccatt tcctgtttgc tctctcgaca acggttagcg tcgctcagaa
     6361 gatcagaccg acccgatcgg gattacgctc agccgtgtgc acctgattca cagtgtcgga
     6421 cacaatcacc tgcccaagcc tcgtctgcaa ttccgtcaag aagacgtcgt ccgacttctt
     6481 tccggcttgt tgagcaatgg tgatgattcg ttggttctct tcttccgcca gtcgatccgc
     6541 cgttaatgtc ccggctcgca ccctgtgcct tgtcaactcc gcgatgagtc ggcccatcac
     6601 ccattgcgta cgagtcgcat gatccaccac catagccccc gcgtaggccg aggcctgtgc
     6661 atacggattc gtaccgtgaa ggcggtatga cgccgccgtg gcctcgttgg gaacattgat
     6721 cgcttcaccg atctcgctcc cataccgctg cttgatcagc gtatcttcca cgatcgcctg
     6781 acccagtatc ggctgaatcc aacgcagcga gacctctaga tcgaccgagc cattcgcctt
     6841 cgcttccggc acagtcgccc cgatcgtgtc gggaagaaag aggaggtgga ggcacagaag
     6901 gattccaccg accgttacag ccatggccat cagtgtcgcg acattcatgc gtttcagtgt
     6961 ccgttccatg tgtcctccat ttcatttatc cgagatcgct caacctcgcg tttgtctgtc
     7021 agactgcaac cgtttcgccc gcagcaactc tcaggtggtc ttctgccttc tcggtgatgg
     7081 cgcgccaggc ttcgatgccg ccggcgagcg cccagacccg cgtgaatcct agcttgcgga
     7141 gcagcgccgt gccctggacg ctcgccgcat cttccgggca gtcgcaatag aggatcacat
     7201 cgcgatcgcg cggaagatcc tgatgcttcg ccttcaggtc ttcgagtgac agagagagtg
     7261 atcccgaaat ccccggttcc tcctgtcgag ctccatgaga acgcaggtcg atgatgatcg
     7321 gatcttctcc agtagcgagt ttgtcggcca cttcctgggc cgtcaggcgc ggcaccagac
     7381 tctccgcttg ccggcggcgg agcgctttca agacgacata cccggtcaca ccgccgagga
     7441 ggattaacat gacggttggg cctacctggg cgctcagggc cagagcgtgt tcgagttcat
     7501 cactgaacag atagccgaga ccgatgccgg atcctaccca caggattgct cccaagccgt
     7561 tgtagaataa gtattgcggc acggggagtc ccacaattcc ggcaagcggc ggcgcaatgg
     7621 tgctcaggcc cggcacaaac ttcgcgacaa ctaatgagcg gactccgtga cgggcaaaga
     7681 gatcttcagt tcgacggacg caagaggtcg gctcgagtga gatgcggcag agccagttca
     7741 acactcgacg acctcgccgt cggccgagtt cgaaccagac ttgatcaccc gcgagagctg
     7801 ccaagacggc tgacccaaca gccatgccta acgccatttg accggtgcca accaaggcac
     7861 cggcggcaat caaaaatggg acagcgggta gcggtaaccc gacttgctcc gcgaagactg
     7921 ttacaaatag gaccagggtt ccgtgctggg ctagaaactc aatcgcttcg ttcataccgg
     7981 cctcgtgtac gtcgaaggtt gtggatggtt gtctgtgctc gcggttcttc ccttggcaat
     8041 gatcgttcca cgcacgtcga caatctcgaa agctgctaag tgacgaaaaa caggaagcat
     8101 tctcctcgcg acacagaata cattgctgat aacgagggaa tggagtcgga gagatactgc
     8161 cgacatctcg gcagatgccg aaaagtcggc gctacttctg acgggagatg ccgagcttct
     8221 gcatcttgga ttggagggtg gttcgtttca taccgagctt ggccgccgcg ccggcctgac
     8281 ctccgatgac ccagttcgtt gcgttcagcg cgcgaacaat gtgttctcgc tctgccgctt
     8341 ccaatgtcga cactgacgcc gcaccagact gagagcgggg ggctttgagt tcgctgacgg
     8401 gaaccaccaa gtcaggaccg gtggagagga tgaccgcccg ttcgaccaga ttttcgagct
     8461 cacgaatgtt tccaggccag gcataggccg ccaaggcatc cattgtatcg gccggtatgc
     8521 gctcgatcga acgattcagg tttcgcgcaa acttctgggc gaagtagcgc acgagcacgg
     8581 gaatgtcctc gcggcgctcc cgcaatggag gaaccatgag cggaaacacg ttcaaccggt
     8641 aatagagatc gctgcgaaat tcttttttct ccaccatctc ggacaggtcc tggttggtcg
     8701 ccgccacgac acgcacgttg actctcatag tcctcgtgct tcccaaccgc tcgaattcct
     8761 gttcctgcag gacgcgcagg agcttcgatt ggatttcgag tgggatgtct cctacttcgt
     8821 cgagaaacag cgtgccgcca tcggccagct cgaatcgtcc gaccttggtg gcaatcgccc
     8881 cggtgaaggc tccgcgctcg tgaccgaaca actcgctttc gagcagaccg gtggggatgg
     8941 ccgcgcagtt catcttcacg aacgtccgct cccgcctgcc gctcaggttg tgaatggcac
     9001 gagccagcaa ttccttcccc gttcccgttt ctcccagaac cagcacggcc gaatccgtcg
     9061 gcgccacgac ttcgacctgc ttcagcacct ttttcaatgt cggactttca ccgatgatct
     9121 cgtcgaagtt gtattcggtc ctgatttctt cttccagata cagtttctcc ttggccagtt
     9181 tctccttgag gccggagatc tccccatagg ccagggcatt ctcgaccgcg atggcgactt
     9241 gcttggccac ctgttgcagg aattcgatac tcgcgctgcc gtaatgatgt ttttcaaagc
     9301 ttccgacgga caacgcaccc aggcgtcggc gcgcggtcgt cagaggaacc gtacagaacg
     9361 accggacgcc tgcctcgcag atcatctgga tcagtttcgg gaatcgggtt tcctgttcga
     9421 tattctcgat caccaggggc tgctggtgtt gccagaccca ccctccaacc gactcttcaa
     9481 cgggaaattc acaccctgga gcgatgctga ccggtcgcgg cgtctccagg atatggagac
     9541 gcatgacgtt ccgctcggga tcatgcagaa tcaaagcgat ataggaaaac tcgagcgcgc
     9601 gcggaagccg ttgggccaga tcgtggaaca gctcggccag gtcacggtgt gacgcaatag
     9661 actcggacac ttccaggagg agacgcaagc ggtcacgatc atgggtcagg tcttgatgat
     9721 ggaggacgtt atccacggcc acggcgactt ggtttccgac ctgtcgcaga aattccagat
     9781 ccgcttcgcc atagacccct ctctccaagc tcgaaaatcc catcgccccc aaccgtcgga
     9841 ccgccgtagt caggggaacg acacaaaacg acttcgtgcc gtcctcctgc atacgttgag
     9901 tcactttcgg ccatcgatgc tcctcggcaa gatccggcac gaggatgggt tgctgcgtct
     9961 gccacaccaa gcctgccgga gtttcctcaa ccggttcctc atggccaccg atcaggtccg
    10021 ccggtacgtt cgcctggatg gtgtgcagtc gcatgatatt gcgcgtgggg tcgtgcaaag
    10081 agagagacac aaaattcact tgcaccactc gggggaggcg ctgggcgagg tcccgaaaga
    10141 gttcgtgcag atcgcgatgc gcggagaccg cctccgcaac ctccaacaga gcctgatacc
    10201 gttc
//
LOCUS       DATDNW010000016         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_412241, whole genome shotgun sequence.
ACCESSION   DATDNW010000016 DATDNW010000000
VERSION     DATDNW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412241"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VI359_00415"
     CDS             complement(<1..1073)
                     /locus_tag="VI359_00415"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY6972742.1"
                     /translation="MGNLVVVGTQWGDEGKGKIVDILARDADVVVRYQGGSNAGHTVI
                     NERGTFIFHLIPSGILYRGTLCVIGNGVVVDPAALIEEMDHLQKQGVKIGKNFAISER
                     AHLILPYHKAIDKASEQSKGARRIGTTGRGIGPSYADKMARIGIRMGDLLNPQAFRKK
                     LEENLVEINWFLEQLYKVDRFEVDKVFQLYMGYAERLKSSIVDGAVLINKVIDAGKTV
                     LFEGAQGTHLDVDMGTYPYVTSSSATAGGAAVGTGVGPTKIDAVMGVVKAYTTRVGSG
                     PFPTELTDDVGGGLQERGKEFGATTGRARRCGWFDGVVARYATMVNGMTSLAVTKLDV
                     LDGCKELKLCTGYRYNGRVFRDMP"
     gene            1436..1511
                     /locus_tag="VI359_00420"
     tRNA            1436..1511
                     /locus_tag="VI359_00420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1469..1471,aa:Ala,seq:ggc)
     gene            1610..3451
                     /locus_tag="VI359_00425"
     CDS             1610..3451
                     /locus_tag="VI359_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY6972743.1"
                     /translation="MLQIESVHKQFATKVLLDGASAHLRPETRVGLVGPNGVGKTTLF
                     KMILGEESPDEGTIRTRPRLRVGYLPQELETISGKTVLDAAHRDQYPEHEAKRILSGL
                     GFSESDFGRPVENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFEEFLLS
                     SKLTLLVISHDTAFLDRITTHIWEIRDKTIQEFRGNYSKFKELRAARDAQLEAAANRQ
                     AKEVARVQKFIDRFRYQANKASQVQSRIKQLEKVKRIELQRDAKRVRFKFPLPSTSGR
                     HVLELTGAAKAYGEKVVYQSLDFSIERGQRIALVGENGAGKSTLLKMLAGVLPLDKGK
                     RTVGHGVTLHYFAQHQAETLNPEHTILESLSEVSKQVEMNFLRGLAGAFLFSGPDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDVLTDALTEFPGTIIFIS
                     HDPTFLTRIATRVVEIEDGQARNFFGDYEYYLWKKAQEFESIKETSEELEGKGVSRTS
                     GPTRAMNHQASAKPSGGERRDLNKTQARLEKQVTRAEAEIAEYEAKIKARDQELADPA
                     LYQAFGKWHDLHLEQETWKKELERLTSRWESLSAELVGIKQKLEAIS"
     gene            complement(3448..>3844)
                     /locus_tag="VI359_00430"
     CDS             complement(3448..>3844)
                     /locus_tag="VI359_00430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6972744.1"
                     /translation="RLMKRQESKPTAAVLASHERRKFVRATLVGSALVSPKTGAKAIT
                     AVLDNVNRIGAGLHAKDRLSMGEKVTVSLAFLDSERAEQQEKLDGTVAWVKPWEKGFL
                     IGVTWDEVVTKEKNRWLNYYLEETIKSTI"
BASE COUNT          871 a         1132 c         1022 g          819 t
ORIGIN      
        1 ggcatgtctc gaaagacccg gccgttgtag cgatatcccg tgcagagctt caattccttg
       61 cacccgtcca agacatcgag cttcgtgacg gcgagcgagg tcatgccgtt caccatggtg
      121 gcgtagcgtg cgaccactcc atcgaaccac ccgcatcgac gggcacgccc ggtcgtcgcc
      181 ccgaattctt tgcctcgctc ttgcagccct ccacccacat catccgtgag ttccgtggga
      241 aagggcccgc tgcccactcg tgtcgtatag gccttcacga cgcccatcac ggcatcaatt
      301 ttcgtcggac ccaccccagt tcccaccgcg gcacctccgg ctgtcgcact ggacgaagtg
      361 acatagggat aggtccccat gtccacgtcc aagtgcgtgc cctgagcgcc ttcgaagagc
      421 acggtcttcc cggcatcgat caccttgttg atgagcacag ccccgtcgac gatagaactt
      481 ttgaggcgtt cggcatatcc catgtacagt tggaagacct tatccacctc gaagcggtcg
      541 accttataaa gttgctccag aaaccagttg atctcgacca gattctcttc gagctttttt
      601 cggaaagcct gggggttgag cagatcaccc atgcgaatgc cgatgcgcgc catcttgtcg
      661 gcgtaggatg gaccaatgcc gcgaccggtc gttccgatgc ggcgggctcc cttcgattgc
      721 tcggacgcct tgtcgatggc tttgtgatag ggcaggatca gatgggcgcg ctcgctgatg
      781 gcgaagttct taccgatctt gactccctgc ttttgcagat gatccatttc ctcgatcaag
      841 gcggcgggat caacgaccac gccgttgccg atgacgcaga gcgtcccgcg atagagaatg
      901 ccggatggga tcaagtgaaa gatgaatgtg ccccgttcat tgatgacggt gtgccccgcg
      961 ttggaacccc cttggtagcg cacgacgacg tccgcatcac gggcgagaat atcgacgatc
     1021 ttccccttgc cttcatcgcc ccactgcgtg ccaacaacaa ctagatttcc catcccgact
     1081 cctcaaaaaa acagccctga ccgaaaaatg gggtcagagc caggcgtcat ggtaggagcc
     1141 ccccaaatgt ttgtcaagcc gatcattgcg acttcttcta cctcgttcac ataccttccc
     1201 cgccaagaga cgagccgtaa tacccttgga ccgcgcgcaa tattctcacc cacccagcct
     1261 gggctcgtcg agacgcgccc tttgcccaag cgggcacctt ccgagcatgc gcggtgcacg
     1321 agcacaacgg atgccctgcc attccgcact ctcccgttcc ttgactggcc ctagggtgca
     1381 tgttagcatg agcctgattt cacagtgttt ttcgctgttt tctttatacc agagtggggc
     1441 tgtggcgcag ttgggagcgc gcgtgaatgg cattcacgag gtcgccggtt caatcccggc
     1501 cagctccacc aaactttcac aacgagctcg agcgtcagtg attcgccttc cgactcctct
     1561 cgtctaagtc gacgcactcc tccagcttca atgccgttga attgcgtgta tgctacaaat
     1621 cgaatctgtc cataagcagt ttgcgacgaa ggtcctgctc gacggcgctt ccgcgcattt
     1681 gcgacccgag acccgtgtcg gcctcgtggg accgaacggc gtcgggaaaa cgaccctctt
     1741 caagatgatc ttgggcgagg aatcaccaga tgagggcacg atccgcacac gtccccgcct
     1801 ccgggtcggc tatcttccgc aagagttaga aaccatctca gggaaaaccg tcctggatgc
     1861 ggctcatcga gaccaatacc cggaacatga agccaagcgc attttgtccg gactcggttt
     1921 cagtgagagc gactttggac gaccggttga aaatctttcc ggtggctatc ggatgagagt
     1981 cgccctcgcc catctgctgt tgtccaatcc ggacgtactg atgctcgacg agccgacgaa
     2041 ccatctcgat aaaccgacgc aacggtggtt cgaagagttt ctcttgagct cgaagttgac
     2101 cctgctggtg atcagccacg atacggcgtt cctggatcga atcaccacac atatctggga
     2161 aattcgagac aagaccattc aagaattccg aggaaactac agcaagttca aagaactacg
     2221 cgcggcgcgc gatgctcaat tggaagccgc cgcaaaccga caggccaagg aagtggcgcg
     2281 ggtccagaaa ttcattgatc gctttcgcta ccaggccaac aaggccagcc aagtgcagtc
     2341 tcgcatcaaa cagctcgaga aggtcaagcg gatcgaactg caacgcgatg cgaaacgcgt
     2401 tcgattcaag tttcctttgc cttcgacgag cgggcgtcat gtcctcgaat tgaccggcgc
     2461 agcaaaagcc tatggtgaaa aggtggtcta ccaatcgttg gacttctcca tcgagcgcgg
     2521 ccagcggatc gccttggtcg gcgagaacgg cgccgggaag agtaccttgt tgaagatgct
     2581 ggccggtgtg ttaccactcg ataaaggaaa acgtacggtc ggtcatggtg tgacgctcca
     2641 ctactttgcc cagcatcagg ccgaaactct gaatcctgaa catacgatcc tggaatccct
     2701 gtccgaagtg tccaaacaag tggagatgaa ctttctgcgc gggctcgcag gcgcatttct
     2761 cttttccggg ccggaccaga aaaaaccgat caaggcatta agcggaggag aacggaatcg
     2821 tgttgccctc gccagaatgt tggtggaacc ggccaatacg ttgcttctcg atgagccgac
     2881 caaccacttg gatccagctt ctgtcgacgt actcacagat gcgctgacgg aattccccgg
     2941 caccatcatc tttatttccc acgatcccac attcttgacc cgcattgcca cgcgcgtcgt
     3001 cgaaatcgag gatggccagg cgagaaactt ctttggcgat tatgagtact atttatggaa
     3061 gaaggcccag gaattcgaat cgatcaagga aacgagcgag gagttggaag gaaagggagt
     3121 gtcgagaacc tctggaccga cacgcgccat gaatcaccag gcgtcggcaa agccgtcagg
     3181 aggagagcgc cgcgacctca acaaaacgca ggctcggctg gagaagcagg tgacacgcgc
     3241 tgaggctgag attgccgagt acgaggccaa gatcaaagca cgagatcaag aattggctga
     3301 cccggcactc tatcaagcat tcggcaagtg gcacgatctc catctcgagc aagaaacatg
     3361 gaagaaagaa ttagaacgtc tcacctcacg gtgggaatca ctgtctgcag aacttgtggg
     3421 gatcaaacag aaactggagg caatcagcta aatcgtcgat ttgatggtct cttcaaggta
     3481 gtaattcagc cagcggtttt tctctttggt gaccacttca tcccaggtca ccccgatcaa
     3541 gaatcctttt tcccatggtt ttacccacgc gaccgtcccg tcgagcttct cttgctgttc
     3601 cgcccgttcc gaatccagaa aagccagcga gacggtgact ttttctccca ttgagagacg
     3661 atccttggca tgaagaccgg ctccgattct attgacgtta tctagcacag ccgtgatcgc
     3721 cttggcgccg gttttgggag ataccaatgc cgatccaacg agtgtcgcgc gaacaaactt
     3781 gcgccgctcg tgcgacgcca ggacggcagc tgttggcttg gattcctgac gcttcatcag
     3841 tcta
//
LOCUS       DATDNW010000017         4198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_616600, whole genome shotgun sequence.
ACCESSION   DATDNW010000017 DATDNW010000000
VERSION     DATDNW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616600"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VI359_00435"
     CDS             complement(<1..414)
                     /locus_tag="VI359_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEY6972745.1"
                     /translation="MAFPSTACLVQQLLACRSVPAFDISASCSGFLYGLSMADAMIRS
                     GQLKTCLVVAAEVKSRTLDRTDVDTALLFGDGAGAVVVRAEPDSSHPSRGLLGVRLHA
                     DGSGHGFIRIPAGGSRTPTTQETVKSKGHTLRMKGA"
     gene            821..1759
                     /gene="bamD"
                     /locus_tag="VI359_00440"
     CDS             821..1759
                     /gene="bamD"
                     /locus_tag="VI359_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY6972746.1"
                     /translation="MRRPLALKPRKPVRIALRAIGYLAVCCMLAACSSTPKNQESSKK
                     ALNGTDEQIFVGDTIEKNYDPNVIMKRGEAFFDKEEYGEAIVEYNHFLDLHRTHMLAP
                     YAAFRIGESQMKRAKGIHRDPEPIQKAIEAFERLRKEYPGNRYEAQAVEKLQECHDLL
                     AQAHLVVGQFYYRRASYLAAAHRFEQIMKLYPDKSVAPEALYFLALSYHDLGADDWAS
                     EQLTLLAEKYPNSKHDGEARRLLAKIGNGNSSTLLAKKSDTGLSGPPPVATESETRIN
                     PPSSFTPQAVTGSVGIPSANALIPSSFVTCRLGAWC"
     gene            complement(1914..2660)
                     /locus_tag="VI359_00445"
     CDS             complement(1914..2660)
                     /locus_tag="VI359_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY6972747.1"
                     /translation="MTQSLPQISVIAAFSAGLLSFVSPCVLPLVPSYISYITGLSVEQ
                     LTDATERSKFKKAIIVNALLFIAGFSTVFIMFGASASFLGQLLITYQDHIRRIGGVLI
                     VVFGLYLLGILNLNFLQMEHRYQFRNRPTGYLGSFLIGVAFAAGWTPCVGPVLGTILL
                     YASTTDSLTSGVSLLTAYSLGLGLPLFITALGVDRFLVYFKQARAYLWGVSTVSGVLL
                     VVVGVMIYANTLTMITSFLERYGIGWYLGQ"
     gene            complement(2665..3216)
                     /locus_tag="VI359_00450"
     CDS             complement(2665..3216)
                     /locus_tag="VI359_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6972748.1"
                     /translation="MTRLKLMWAALTLVAFLPGAYGASPFVDTSVGSVAASRGRVKPG
                     DAAPNFQLRDLDGKAVSLSDFLGKVVLVNFWATWCGPCRVEMPAMEQLYRTFPRKDFE
                     ILAVSTDPQGAAVTRPFQQEIGLTFPILHDADYRVGLTYGARSLPMTFMVDRNGVVRQ
                     QIFGARDWSAPEAHQLIQVLMKS"
     gene            complement(3228..3875)
                     /locus_tag="VI359_00455"
     CDS             complement(3228..3875)
                     /locus_tag="VI359_00455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6972749.1"
                     /translation="MSILQDSAEEKVLSESPIPTSSRAVPPVSGSRLLILLAGVIILG
                     AVFGIVWMQSAKYEPLAVGKPAPDFALSDLTDKPYRLSDFRGKVVFLNFWATWCKPCR
                     EEMPSMEVLYKNFEKDGLVILAVSIDRVTTTKDIPPFVKGMNLTFPVLIDSWGQTDKP
                     YKRMGVPETFIIDQQGVIQEIVIGPRDWTRLDSLPVITKLLNVTPKTVDAGRPSM"
     gene            complement(4033..4188)
                     /gene="ccsA"
                     /locus_tag="VI359_00460"
     CDS             complement(4033..4188)
                     /gene="ccsA"
                     /locus_tag="VI359_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY6972750.1"
                     /translation="MWLIYGAYIHARMTRGWEGRRAAIYAVFGFLMVIFCFWGVNFLL
                     SGLHAYA"
BASE COUNT         1038 a         1247 c         1112 g          801 t
ORIGIN      
        1 ggcccctttc atccgcaacg tgtgcccctt tgatttcacc gtctcctgtg tcgtcggggt
       61 tcgggaccct cctgcaggaa tgcgaatgaa tccatgacct gatccgtcgg catggagacg
      121 tacacccaaa aggccccggc ttggatggct gctatcgggc tctgcccgga ccactacggc
      181 cccggcccca tcaccaaaca agagcgccgt gtcgacatcc gtgcgatcca acgtccgcga
      241 tttcacctcg gctgccacca ccagacacgt cttgagttgt ccacttcgaa tcatggcatc
      301 cgccatcgag agtccataga ggaatccgga gcatgaggcc gagatatcga aggccggcac
      361 actccggcaa gccagcaact gctgaacgag gcaggccgtt gacggaaatg ccatatcagg
      421 tgaggtcgtc gaaaccaaaa ttccttccaa atcagcggcc gtaagacctg cgcttgccaa
      481 cgcctgtcga cctgcctccg cagccagatc tgagggcgct tgcctaggag atgcccagcg
      541 ccgctccctg atcccggtga gtcggtgtac aacggccgca tcaaccccca ctggttcgcc
      601 aacctcgcga ttggagatca cccgttcggg gagatatgag cctgtcccaa ttattctggt
      661 ccgtaccatc ctggatttgt ccactcctcc gggaggccat ctagcccccg gtgccggcgg
      721 gattataggt ctcagtgaga aaggggtcaa ccaaccgtcg gaatcattca tcggaatcat
      781 tgcatttcgc cagcccccct gctacaaaag gagccaggct atgagaagac cccttgcgct
      841 aaaacctcga aaaccagtga gaattgccct ccgagcgatc ggctatttgg ccgtctgctg
      901 catgctggcg gcctgttcca gcacgcccaa gaaccaagaa tcctccaaga aagccttgaa
      961 cgggaccgat gagcagattt ttgtgggaga cacgattgaa aagaactacg accccaacgt
     1021 gatcatgaaa cggggcgaag cctttttcga taaggaagaa tatggggaag cgatcgtcga
     1081 gtataaccat tttctcgatc tccaccgcac ccacatgctc gccccctacg ctgccttccg
     1141 gatcggcgaa agccagatga agcgagccaa gggaatccac cgggaccccg aacccattca
     1201 aaaggcgatc gaggcgttcg agcggttacg aaaggaatac ccggggaacc gctacgaggc
     1261 tcaagccgtg gagaaacttc aggaatgcca cgacctcctg gcacaggccc atctcgtcgt
     1321 gggacaattt tattatcggc gcgcctcata tttggctgcg gcccaccgct tcgagcagat
     1381 tatgaaactt tacccggata agtcggttgc gccggaagcc ctctactttc tggccttgag
     1441 ctatcacgat ctgggagccg acgactgggc cagcgagcag ttgaccctgt tggcagaaaa
     1501 atatcccaac agcaaacatg atggtgaagc ccgacggtta ttggccaaga tcgggaatgg
     1561 gaattcctcc acgctgctgg caaaaaaatc cgataccgga ctgtcaggac caccccccgt
     1621 cgcaaccgaa tcggagactc gcatcaatcc cccttcttca ttcaccccgc aagccgtgac
     1681 cggttcagta ggaatcccgt ccgccaacgc cttgataccg tcgtcgttcg tgacgtgccg
     1741 actcggcgcc tggtgctagc agctacacca cgcaactaag aagctgtcag cgttcagcta
     1801 taagctgttc ggcagaggct gagggctgta gaggatgagt cagctaacgg aattaccgaa
     1861 attcggcaag tacggtaaca tcttctgctg aaggctgaaa gctgatggct aggtcattga
     1921 cccaggtacc agccgatgcc ataccgctcc aagaaactgg taatcattgt caacgtgttg
     1981 gcataaatca tcacgcccac cacaaccagc aacaccccgc tcaccgtcga gacgccccac
     2041 aaataggccc gtgcctgttt gaagtagacc aagaagcggt ccacaccgag cgctgtgatg
     2101 aagagcggca aacccaagcc gagtgagtac gccgtcaaca agctgacgcc gctcgtcagc
     2161 gaatcggtcg tgctggcata gagcaaaatg gttccgagga cggggccgac acacggagtc
     2221 catccagcgg caaacgcaac accgatgaga aacgatccga ggtagccggt cggacgattt
     2281 ctaaattggt accgatgttc catctgcaga aaattcaaat tcaggatgcc caagagatac
     2341 agaccgaaca cgacgatcaa tactcctccg atccgtctga tgtggtcttg gtaggtaatt
     2401 aacagctgac caaggaaact cgcagaggcg ccgaacataa taaagacggt cgaaaagccg
     2461 gcgatgaaca agagcgcatt gacgataatg gctttcttga acttggatcg ctccgtcgca
     2521 tccgtcagtt gttccacaga caacccggta atgtaggaaa tatacgaagg gacgagcggc
     2581 aatacgcagg gcgaaacaaa tgaaagcaag cccgcggaaa atgcggcaat cacggagatc
     2641 tgtggaagag attgcgtcat gctcttacga cttcatgagc acttgaatca attggtgcgc
     2701 ttccggcgca ctccagtcgc gtgcgccaaa gatctgttgt cggacaactc cgtttcggtc
     2761 gaccatgaat gtcatcggca agctgcgcgc accataggtc agccccaccc gatagtcggc
     2821 atcatgcaag atgggaaacg tcaggccgat ttcctgctga aacggacggg tcacggcggc
     2881 tccttgagga tcggtggaca ccgcgagaat ttcaaaatct tttcgaggaa aggtgcggta
     2941 gagttgctcc attgccggca tctcgacgcg gcagggccca caccatgtcg cccaaaagtt
     3001 cacaagaacc actttcccaa gaaaatccga caacgagacg gcttttccat caagatcccg
     3061 caattgaaaa ttcggcgcgg cgtcgcctgg tttgacgcga ccacgcgacg cggcaacaga
     3121 acctacgctc gtgtcgacaa acggtgaggc cccataagct cccggtagaa acgctaccag
     3181 ggtcaaggca gcccacatca atttgagtcg cgtcatccct tcctcactta catcgacggc
     3241 cggccggcat cgaccgtctt gggcgtcacg ttcagcaact tcgtgattac cggcaggcta
     3301 tcaagtcgtg tccagtctcg tggcccgatc acaatttctt ggatgacacc ctgttgatcg
     3361 atgataaacg tctcaggcac acccatccgt ttgtagggct tgtcggtttg tccccacgaa
     3421 tcgatcaaca ccggaaaggt cagattcatc cctttgacga acggagggat atccttcgtc
     3481 gtcgtgacgc ggtcgatgct cacggccaag atcaccaggc cgtccttttc aaagttttta
     3541 tagagcactt ccatcgacgg catttcctcg cggcagggct tgcaccaggt tgcccagaaa
     3601 ttgaggaaca ctactttgcc gcgaaagtca gagagtcggt agggcttgtc ggtgagatcc
     3661 gataacgcga agtccggcgc cggcttgccg accgctaatg gctcatactt ggcgctctgc
     3721 atccatacaa tgccaaatac ggcgccgaga atgatcaccc ccgccagcaa gatgaggagg
     3781 cgggagccgc tgacaggagg tacagcacga gatgatgtag gaatcggaga ctccgacaag
     3841 actttttcct ccgcactgtc ttgtaggatg ctcaaaatgc tgccttctca cccgcccacc
     3901 ccggcgcgcc gagacgcgcc gtccaccgag catggccgcc gggaacgagg gccccgaggc
     3961 gtacagtcgt tagtacgttg aggggagcga gcgaccgaga acgaagttgg tagccatttt
     4021 cagcatccac ggctacgcgt aggcgtggag acccgacagc aaaaagttca caccccagaa
     4081 acagaagatc accattaaga atccgaagac cgcatagatg gccgccctgc gaccttccca
     4141 cccacgcgtc atgcgcgcat ggatgtaggc tccgtaaatc agccacacga tcaacgac
//
LOCUS       DATDNW010000018         3501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_138763, whole genome shotgun sequence.
ACCESSION   DATDNW010000018 DATDNW010000000
VERSION     DATDNW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138763"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VI359_00465"
     CDS             <1..364
                     /locus_tag="VI359_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6972751.1"
                     /translation="YPWPGNVREMENIIEQAFIWSKGSATITPEHLPNILRNDTRSSS
                     LRDDTLAGRLSLEKAVMEFEREIILDALKRTNYVQTHAANLLGISRRMLKYRMDTLGI
                     GRPDNEVGGEPQPPPVQE"
     gene            583..1719
                     /locus_tag="VI359_00470"
     CDS             583..1719
                     /locus_tag="VI359_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6972752.1"
                     /translation="MRDTERTKPTVLVIDDEAGPRDALKVILRPFFNIQSAESAKAAI
                     DVLNSQPIDLITLDQKLPDRQGLDLLQDIKHTHADVEVIIITGYGSLKSAMEGIRHGA
                     AGYLLKPFNVTELITLVNQTLEKKQRLDFLRSFLSTKSALWGSEQESAQAWKELMAEY
                     YRIGNKASADTGTTGETSDVLPLLSDLLEAKDRQLLNHCSRVSFYATLLANRMNLTMV
                     EQKSLALGAFLHDIGKVASEVYRFADDEISQAGETTSNHQHPELGARMILPLGLPAEV
                     GQIVSYHHERWDGSGYPHGLQGEGIPVLARIVCLAQAFDHLTAELPGRTPMTIDRASQ
                     NMVQHAGTFFDPMLTELFTRVVDECKASLPAMAIATTPTTEPDA"
     gene            complement(1726..3411)
                     /gene="recN"
                     /locus_tag="VI359_00475"
     CDS             complement(1726..3411)
                     /gene="recN"
                     /locus_tag="VI359_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY6972753.1"
                     /translation="MLAELRIANFALIEQLNLQFQPGYTVLTGETGAGKSLLIDAIGL
                     LVGGRASADQIRSGEEEAILEAAFQLTSPHPLLEQLRAKGLVGADDTELILRRVLSRS
                     GRHRVFVNGSLCPLRTLEELGGTLVDILGQHEQQSLLSHGAQLEALDAFGRTLDLRAR
                     YQQAYQTWNDLRVQAAVVQAEAADRAQRAELLRFQHQEIEQAGLQPGEDEILQTERQR
                     LVHARRLGELAQAAYEGLQGDEQGVLSHLTRVVRALSDLVHTDVSMQDCQQAATEAMI
                     QLKDLAGQLRDYTENLESDPSRQTAVEDRLDLIQRLKKKYGGSIVTVVEFGERVKGEL
                     EAIATHNDTMAVLTAKVEEARESLWELALQLSKKRAEAAKRMKTFVKEELAVLKMDQA
                     AFDIVVTSAEAIESAGPSGRDDVEFLISTNRGEPLKPLARVASGGELSRVMLALKTIL
                     AEMDQVPVLVFDEVDSGVGGAVAAAMGTRLRRLGAFHQVFCITHLPQVASQADHHLVV
                     EKEQNRKRTTASVRTLTGIGREAEIARMLGGETVTQKVRATAAELLSETKPRR"
BASE COUNT          807 a         1046 c          872 g          776 t
ORIGIN      
        1 ctatccctgg ccaggcaatg tgagagaaat ggagaacatc atcgaacagg cgtttatttg
       61 gtcaaaagga tccgccacca tcacgcctga acatcttccg aacattctcc gcaatgacac
      121 tcgttcatct tctcttcgcg acgacacact ggcggggcga ctttccctcg aaaaagccgt
      181 gatggagttc gaacgcgaga tcatcttgga cgctcttaaa cggaccaact atgtacagac
      241 ccatgccgcc aacctcctgg ggattagccg tcgaatgctg aaatatcgaa tggacactct
      301 cggaattggc cgacccgaca atgaagtcgg cggcgaaccc caaccaccgc cagtgcagga
      361 ataacacagc ctcttgagag gattgagctt tttttgcacg gagcgggtac agaactgccc
      421 gtctgctacg tagagattcg tctgtggaaa ctacatatag ttgccctcca atcacaccct
      481 acaccagctt gatgtcagac acataagtca ctgaatcgga acaggaattg cttgacataa
      541 tacgtacttg ggggtagagg acactacatg agcggtgcct ccgtgagaga tacagagaga
      601 acgaagccga ccgtcctcgt gattgacgac gaggcggggc cacgtgatgc ccttaaggtt
      661 atcctgcgtc ctttcttcaa tatccaatcc gcagaatcgg ccaaagccgc cattgacgta
      721 ctcaactcgc aacctataga cctaataacc ctcgatcaga aactccccga cagacagggc
      781 ttggatctct tacaagacat caagcatacc catgcagacg tcgaagtcat catcatcacg
      841 ggctacggca gtttgaaatc cgcgatggaa ggcattcgac acggtgcagc tggatacctc
      901 ctcaagccgt ttaacgtcac ggaacttatc actctcgtca accagacgtt agaaaaaaag
      961 cagcgcctcg acttcctgcg aagttttttg agcaccaagt ctgcactctg gggatctgag
     1021 caagagagcg ctcaggcgtg gaaagaattg atggcagagt attatcgtat cgggaacaag
     1081 gcgtcggccg acacagggac caccggcgag acgtcggacg tccttccatt actttccgac
     1141 ctgctggaag cgaaggatcg ccagctgctc aaccactgca gcagagtcag tttctacgca
     1201 acgcttttgg ccaaccggat gaatctcacc atggtggagc aaaagtcgct cgctctcgga
     1261 gcttttctcc atgacatcgg gaaggtcgca tcagaagtat accgatttgc cgacgatgaa
     1321 atttcccaag ccggggagac cacgagtaat caccagcatc cggaacttgg tgctcgcatg
     1381 attctccccc ttggtcttcc tgccgaagta gggcagatcg tgtcctacca tcacgaacgg
     1441 tgggatggtt caggttatcc gcatggtttg caaggagaag gtatccctgt attggctcgt
     1501 atcgtgtgtc tcgcacaagc ctttgaccat ctcacggcag aacttccagg ccgcactccc
     1561 atgaccatcg accgcgccag tcaaaacatg gtccagcatg ccggaacgtt ctttgatccg
     1621 atgctcaccg aactttttac gcgcgtggtt gatgaatgca aggcctctct tcctgcgatg
     1681 gccattgcga ccaccccaac aactgaacct gacgcttaag tgcgattatc gtctcggctt
     1741 cgtctcagat aaaagctcgg ccgcagtagc ccgaactttt tgcgttacag tctcgccccc
     1801 aagcatccga gcgatctcgg cttctcgacc aatgccggta agagtcctca cggaggccgt
     1861 ggttcgcttt cgattctgtt ccttttccac aaccagatgg tgatcagcct gagatgcgac
     1921 ttgcggcaaa tgtgtaatgc agaatacttg gtggaacgcg cccaacctcc gcaatcttgt
     1981 tcccatcgca gcggctacgg cacctccaac acctgaatcc acctcatcaa aaaccaagac
     2041 tggcacctga tccatctccg ccaagatcgt tttgagggcc aacatgaccc ttgagagttc
     2101 ccctccggaa gctacacggg cgaggggctt caaaggttcc cctcgattcg tcgagatgag
     2161 aaactcgacg tcgtctcggc cgctcggtcc ggccgactcg atggcctctg cgctcgtcac
     2221 gacaatgtcg aatgccgcct ggtccatctt caaaacagcc aattcctctt tcacaaacgt
     2281 tttcattcgt ttggcggcct cggctctttt cttggaaagt tgcagggcca gctcccacag
     2341 agactctcgg gcctcttcca ccttcgctgt caacacggcc atcgtatcat tgtgcgtcgc
     2401 aatggcctcc aattctccct tcactcgttc accgaactcc acgactgtta cgatcgatcc
     2461 tccgtacttc ttttttaacc gttggatcag atcgagccga tcttccaccg ccgtctgtcg
     2521 gcttgggtca gattccagat tttccgtata gtcacgaagc tgcccggcga gatctttgag
     2581 ttggatcatc gcctcggtcg ccgcctgttg gcagtcctgc atcgacacat cggtatggac
     2641 caggtcagac agagcccgaa cgaccctggt caagtgactg aggacaccct gctcatcgcc
     2701 ctgtaacccc tcgtacgcgg cctgcgccaa ctcacctaat cgacgggcat gcaccagtcg
     2761 ctgccgttca gtttgtagga tttcgtcctc acctggttgc aatcctgcct gttcgatctc
     2821 ttgatgctgg aaccgaagca actcggcccg ttgggcacga tcggcagctt ccgcctggac
     2881 aactgcggcc tgaaccctca gatcgttcca tgtctggtac gcctgttgat agcgcgcacg
     2941 aaggtcgagc gtccgtccaa acgcatccag cgcctcaagc tgcgcaccat ggctcaacaa
     3001 tgattgctgt tcgtgttgcc cgagaatgtc aacgagggtt cctccgagct cttccagcgt
     3061 gcggagggga cacaagcttc cgttcacgaa aacgcgatga cgaccggaac gggagagaac
     3121 gcgccgaaga atcaactccg tatcgtccgc gccaacaagc ccttttgccc gcaattgctc
     3181 gagcaacgga tgcggagagg tcagctgaaa ggccgcctcg agaatggctt cctcttcacc
     3241 cgaacggatc tgatcggctg acgcccgacc gccaaccaac aaaccgatcg catcgatgag
     3301 gagagacttt ccggcaccgg tttcgccggt cagaaccgta tagcctggct ggaattgaag
     3361 attgagttgc tcgatcagtg cgaagttggc gatgcgcaac tcagcaagca ttgcgacgga
     3421 tcatgacgct ccttacgcgg aaccggcatg ttgcgcaagc agcgagtcga tgatttcctt
     3481 gaattgtcgt ttcggccttg c
//
LOCUS       DATDNW010000019         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_70409,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000019 DATDNW010000000
VERSION     DATDNW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70409"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VI359_00480"
     CDS             complement(<1..1263)
                     /locus_tag="VI359_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY6972754.1"
                     /translation="MPTSNWEELAEGALARIKASGAEYGDIRLLDSTVQTITGEDRRI
                     ASLRESTDRGFGIRVLYHGGWGFAASSVCSLEEVPRVADLAVEIARGSASLAIEKVKL
                     ADEPVHRDQVVTTRRIDPFAVSTEKKTTLLLETMELLQQQAGVVRSSASLWACRDRKL
                     FVSTEGTHLEFDLLALNGEMTATASRHGRFATRSYATPHLRTGYELIEDANFLSEAPR
                     IAGQAVEKVNAPAIDAGSYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TTEKRGTFRYGSPHVNLVADNTETTTLAATGYDDDGVACQRWDIVRDGIFVGYCTNRE
                     VAPKIGESRSRGSNRADSWSSVPMVRIANIGLEPGTGSLDDLIVDVKKGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIERGKRTHMVR"
     gene            1356..1664
                     /locus_tag="VI359_00485"
     CDS             1356..1664
                     /locus_tag="VI359_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972755.1"
                     /translation="MLPPDRTPRQRIIELITGTRLSSYQLAQMVGIPERQVEDHLEHV
                     VKTVARDRSRRFILEPSTCQDCGFVFRDRRRLTRPSRCPMCHSEGITAPRYGIDAMSS
                     "
     gene            1869..2093
                     /locus_tag="VI359_00490"
     CDS             1869..2093
                     /locus_tag="VI359_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972756.1"
                     /translation="MNRWCSTALFVLALMLTACTGDKPKELLETAELEERQHNVVHAR
                     QLYEDLIRLYPTSPQAEIARARLTALSQSQ"
     gene            complement(2298..3092)
                     /locus_tag="VI359_00495"
     CDS             complement(2298..3092)
                     /locus_tag="VI359_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY6972757.1"
                     /translation="MTLHDPLTLIGTALVVMTVVMFILWVVQFYGRNASLADVGWCGG
                     LVGVVCWYGIAAFGDPGRRGLVVAMITVYGLRLGSYILLNRVIGKTEDPRYQHMRRWL
                     GTREQLGVFAYFLLQAPAVVLFSLPPLAVMQNPRPTFSLWELAGVLVWLVAVTGEAAA
                     DWQLAHFREKSWNRNRVCRDGLWQYSRHPNYFFEWLQWWAYVLMAVGTPGWLLTCIGP
                     LVMGIALLSVTGIPLAERQALVARGEDYRTYQRTTNAFVPWFPKRQ"
     gene            3370..>3612
                     /locus_tag="VI359_00500"
     CDS             3370..>3612
                     /locus_tag="VI359_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6972758.1"
                     /translation="MASLSPTGSANIPRYQLFIDGKWVDARSGKTFTSPNPSTGGSLA
                     AIAEGDAVDIDIAVRAARKAYEGVWHNVTARDRGRML"
BASE COUNT          758 a         1109 c         1022 g          723 t
ORIGIN      
        1 gcggaccata tgggtccgct taccacgttc gatgagccag aacgcatcgc cgccgaactg
       61 aaagttgtag cggcgctggt cgatgctgta tgacccatgg ccttcaatgt aaatcccctt
      121 cttcacgtcg acgatcagat catcgaggga gccggtgccc ggctctaagc cgatgttcgc
      181 gatacggacc atcggcacgc tgctccaact gtcggcgcga ttcgacccgc gcgaacgaga
      241 ctcaccgatc ttcggtgcca cttcccgatt cgtgcaatac ccgacgaaga tcccgtcgcg
      301 gacgatatcc caccgttgac aggccactcc gtcgtcatcg tagcccgtcg ccgccaacgt
      361 ggtcgtctcc gtattgtccg ccaccaaatt cacgtgcggg gatccgtacc gaaaggtccc
      421 tcgcttctcg gtcgtgagga agctcgtgcc ggcatagttg gcttcgtagc ctagtgcgcg
      481 atccaactcg ctcggatggc cgcaggattc atgcatcgtc agcgaaagat gctcggggtc
      541 gaggacgaga tcataggaac cagcatcgat cgccggagca ttcacctttt cgaccgcttg
      601 gcctgcaatc cggggagctt cggataagaa atttgcgtct tcgatcagtt catagcctgt
      661 tcggagatgg ggcgtggcat aacttcgcgt cgcaaaccgc ccgtgtcggc tcgccgtggc
      721 cgtcatctcc ccgttgagag ccagcaggtc gaactcgaga tgggtacctt cagtggatac
      781 gaaaagtttc cggtcccggc aggcccagag actggctgag ctacggacca cgcccgcttg
      841 ctgctgcagc aattccatcg tttctagaag gagcgtagtc ttcttctccg ttgagaccgc
      901 aaagggatcg atccgtctgg tcgtcaccac ctgatcgcgg tggaccggct catcggcgag
      961 cttcaccttc tcaatggcta acgaggccga tcctctggcg atctcgaccg caaggtcggc
     1021 cacacgcggc acttcctcca gcgaacagac cgaactggcg gcaaaccccc atccgccatg
     1081 gtagagcacc cggatgccga agccgcgatc ggtcgactcg cgcagggagg caatccgacg
     1141 atcttcaccg gtaatggttt ggacggtact gtcgagaagg cggatgtctc cgtattccgc
     1201 accagaagcc ttgatgcggg caagcgctcc ctcggccagc tcttcccaat ttgacgtagg
     1261 catcgtcctg tcatcctgcc aaagttacgc cgcacgttca accagtcatt cgcttcactt
     1321 ggctttggcg catgcgtcgc ttaggataca gacttgtgtt gccacctgac cgtacccccc
     1381 ggcagcggat catcgagttg atcaccggca cccgactctc gtcttaccag ctcgcccaaa
     1441 tggtggggat tccggaacgc caggtggaag accatctcga gcatgtggtc aagacggttg
     1501 cgcgcgaccg gtcgcggcgt ttcattctgg agccatcgac gtgccaagac tgcgggttcg
     1561 tcttccgcga tcgccgccgc cttacgcgac cgagtcgttg ccccatgtgc cacagcgaag
     1621 gcatcacggc acctcgatac ggcatcgatg caatgagttc gtagccgagg tccggattcc
     1681 ccgtccattt tgtgaatgta tttggcctgt tggttctctt ttttagctcc agtgctacgt
     1741 gcccctcgtt tcaccggtgg caaggaggac ctcaattggg gtaagttgtg gcgcgtgcca
     1801 tctcatccta cgatggaacc gatcacgaaa tcatggtagc aggaactcta agacttggac
     1861 tccaatctat gaatcgatgg tgttcgaccg ctctcttcgt tcttgcgctg atgctgaccg
     1921 cctgcaccgg cgacaagcct aaggagttgc tggagaccgc cgaactggaa gagcggcaac
     1981 acaacgtggt gcacgcaagg caattgtacg aagatctcat tcgactctac ccaaccagcc
     2041 cacaagcaga gattgctcgg gcccgtctga ctgctctcag ccaaagccag tagggtgtcg
     2101 aataatcttt ccggctcgat cggaatccgg tagtaccaat gccttgagga ggctgagact
     2161 gcccctcagg atgttcaaaa aggctgtcag cgaggccgca gcgagtgaag aggcgatgcg
     2221 tacttcgctc cgtacgtgga gcctctgagg gatgcgagaa cgaagctgac ggcctttttc
     2281 aacttcctgc cagtgactca ctgccgcttc ggaaaccagg gaacaaaggc attcgtcgtg
     2341 cgctgatagg tccgatagtc ttccccgcgg gcgaccagcg cctgccgttc agccaacgga
     2401 atgccggtca cgcttaatag cgcgattccc atcacgagag gtccgataca cgtcagcagc
     2461 catcccggtg tgccgacggc catcagcaca taagcccacc actgcaacca ttcgaaaaag
     2521 tagttgggat gacgggagta ctgccagagg ccgtcacggc acactcgatt tctgttccac
     2581 gatttctcgc gaaagtgtgc gagctgccaa tctgccgccg cttctcccgt cactgccacg
     2641 agccagacca gcacacccgc gagttcccat aggctgaacg tgggccgtgg attctgcata
     2701 acggccaggg gaggcaggga gaacagcacc acggccgggg cttggagcag aaagtaggcg
     2761 aacacgccca gctgttcccg agtgcccagc catcgacgca tgtgttggta ccgcggatct
     2821 tccgtcttgc cgatgacgcg attcagcaga atataggagc caaggcgcag accgtaaacc
     2881 gtgatcatcg ccacgacgag gccacggcgc ccgggatcgc cgaaggcggc tatgccgtac
     2941 cagcacacaa ctccgacaag cccaccacac catcccacat cggccagcga tgcgttgcga
     3001 ccatagaatt gcaccaccca caggatgaac atcaccaccg tcatcaccac cagcgccgta
     3061 ccgatcaggg taaggggatc gtgcaaggtc ataggctagg gtccgctctg tagagaagga
     3121 gggaggcagt ttcagaggtc attgctgcca attagtcctc gtgcgctcgt cacccgcatg
     3181 agcgatttca tacagagacc gctccgccgg tgacaaagaa aggcctcgac ggtgcgagag
     3241 acttgatgag acgagtcatt atagtgggat ctggtcgggg tgcaagccgt cgcccacacc
     3301 cgaatctcgg ccagtggtat agtgaaagtg taggagctcg tcccttcatt cctatcaagg
     3361 aggtgctcca tggcctctct gtctcccacc ggttcagcca acattcctcg ttatcaactg
     3421 ttcatcgacg gtaagtgggt tgacgctcgg tcgggcaaaa ccttcacgtc cccaaatccg
     3481 tccaccggcg gatcgctggc ggcaatcgca gaaggcgacg cggtcgacat cgatatcgcc
     3541 gtacgcgctg cgcgcaaggc ctatgaggga gtctggcaca acgtgaccgc gcgggaccgt
     3601 gggcgcatgt tg
//
LOCUS       DATDNW010000020         6285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_275361, whole genome shotgun sequence.
ACCESSION   DATDNW010000020 DATDNW010000000
VERSION     DATDNW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275361"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VI359_00505"
     CDS             complement(<1..734)
                     /locus_tag="VI359_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY6972759.1"
                     /translation="MARFRQVRLSGSLLLLMVLTIGVDHSFAGEHKSPGPPRPSTVLA
                     QAATPAPVEGQAGSPEQELPPGGTAVEDLLLQKGLITMDEWIRIKAEQEYRSADQARR
                     IDAIEDWKERTELLPILRDKVNFGLNALQFLYGHSNAQVPEGKSQDSFSIRRAELIFW
                     GKISDTIPRWHTLFEFQSTNLTSNTPTNGTSTPSSTTNFREAYIDFRPIQSWAPNLNY
                     FRMGIFRMPWGIFTETSGGLRDIISSP"
     gene            1068..2042
                     /locus_tag="VI359_00510"
     CDS             1068..2042
                     /locus_tag="VI359_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6972760.1"
                     /translation="MLTTCLFAWPVAPTQSETALLVKVDGSSTVFPITEAVAEEFQKD
                     NHGTIRVTVGISGTGGGFKKFCRGETDVQDASRPISASEMEACRTNGIQYYELPVAFD
                     ALTIAVSPQATWIDAITVAELKQMWEPSAQGRVMKWNQVRSTWPDSPLKLFGAGSDSG
                     TFDYFTEAVTGKAKASRGDYTASEDDNTLVQGISNDKQALGYIPYAYFEPNKMRLKAV
                     AVDGGRGPVLPSRETVENGAYQPLSRPMFIYVSSKSAGRPDVKRFVEFYLSQAPTLVP
                     QVKYVPLPAQAYDLALTHFRNGKLGTAFQGISTIGLKIEDLLRREAKL"
     gene            2085..2999
                     /gene="pstC"
                     /locus_tag="VI359_00515"
     CDS             2085..2999
                     /gene="pstC"
                     /locus_tag="VI359_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY6972761.1"
                     /translation="MRKAADFRIFSRTLFHLKERGIELLLFLAALTSVAITVGIVGVL
                     VYESLTFFRDVSLFDFLTDRQWTPLFSEPHYGIMSLVSGTLVTTSIALLVAIPMGSLV
                     AIYLSEYARPNVREIVKPALELLSAVPTVVYGYFALLFVTPALQKVWPGLPGFNMLSA
                     GLVIGIMIVPYVSSVSEDAMRAVPVYLREGAYALGATRMQTAIRVVFPSALSGIAAAY
                     VLGVSRAIGETMVVAIAAGMQPTLTWNPLEPAATMTAYIVQVSLGDLPHGSVGYQTIF
                     ATGLTLLLMTLLFNIAGHVLRKRYRQAY"
     gene            2965..3909
                     /gene="pstA"
                     /locus_tag="VI359_00520"
     CDS             2965..3909
                     /gene="pstA"
                     /locus_tag="VI359_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY6972762.1"
                     /translation="MFSENDIDKPTSPGLLKAAIDRRKRTDLLFAAVGLSTMLLALVT
                     LLALLGQLGMDGIGRLSLQFLTSFPSRFPAQAGILSAWVGTILVMVLTALTAVPLGLG
                     AAIYLEEYAPRNWVTDLIEINIANLAGVPSIVYGLMALGLFVYQFHFGQSFLTAGLTL
                     GMLILPMVIIATREAIRSVPPQIREAAYALGATKWQTVRDHVVPYSMGGILTGLILAL
                     SRAVGETAPLITVGALSFIAFLPHPPWQPDFPFFSFHWLFDPFTVMPIQMFNWVSRPQ
                     EDFHANAAAAGIILLLMTLAMNALAISLRARFRKRIHW"
     gene            3914..4699
                     /gene="pstB"
                     /locus_tag="VI359_00525"
     CDS             3914..4699
                     /gene="pstB"
                     /locus_tag="VI359_00525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY6972763.1"
                     /translation="MTTSSETRLSLKAEAKGLSFYYGAVRALNDISLGLVDRRATALI
                     GPSGCGKTTYLRCLNRMHDLYAGTRYEGEVLLYPDQVNIVDQSVDPIEIRMRVGMVFQ
                     KPNPFPKSIYENVAYGLRIRQATKRSELNEAVERALRDAALWTEVKDRLHDLASNLSG
                     GQQQRLCIARALATGPEMLLFDEPTSALDPTATARIEELVTELKQRVTIVIVTHNMQQ
                     AARVSDFTAFMYEGKLVEFDRTETIFTNPSNKLTEDYVTGRFG"
     gene            4723..5403
                     /gene="phoU"
                     /locus_tag="VI359_00530"
     CDS             4723..5403
                     /gene="phoU"
                     /locus_tag="VI359_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY6972764.1"
                     /translation="MQRHIDQELAELKTQILRMGGLVEGQIERAIRALVERDVDLASQ
                     VIDRDTLVNSLDVEIDENCIRLLALQAPAAGDLRFVTTAMKISTELERMSDLAEIICE
                     RVIELNEEPQLKPYIDIPRMANWTMRMVRECLDAFVRSDSALARKVCTDDDFVDNLTH
                     QLFRELLSFMIENPGTISRAIRLTFIGKYIERIADHATNVAELVVYMVEGKIVRHIDP
                     PALLDKNS"
     gene            6081..>6285
                     /locus_tag="VI359_00535"
     CDS             6081..>6285
                     /locus_tag="VI359_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972765.1"
                     /translation="MTPIDHLMMDAKQAIMEEQHRRFQDLHREGRWVEALQQMHVTLS
                     CASDLLNESLQALDKALGSCHAPS"
BASE COUNT         1465 a         1660 c         1660 g         1500 t
ORIGIN      
        1 ggcgagctga tgatatcgcg caacccaccg gaagtttctg tgaagatgcc ccagggcatc
       61 cggaagatgc ccatccggaa gtaattcaag ttgggagccc aagactgaat cggtcgaaaa
      121 tcgatatagg cttctcggaa attcgtcgta gacgacggag tcgatgttcc gtttgttggc
      181 gtgttgctag ttaagttggt actctgaaac tcaaagagcg tgtgccaccg ggggatggta
      241 tcactgatct tcccccagaa gatgagctcc gctcgccgaa tggaaaaact gtcctggctt
      301 ttcccttccg gaacttgcgc attgctgtga ccatagagga actgaagggc attgaggcca
      361 aaattcactt tatctcttag gattggcaat agctcggttc tctccttcca atcctcaatc
      421 gcatcaatac gcctagcctg gtcggccgat cgatactcct gctccgcctt gatccggatc
      481 cattcatcca tcgtgatcag gcccttttgc aggaggagat cttcgactgc ggttcctccc
      541 ggtggcaact cttgctctgg agaaccggct tgcccttcaa ccggagccgg tgtggccgct
      601 tgggcaagga ccgtcgaagg ccgcggtggc cccggactct tatgttcacc ggcaaacgaa
      661 tgatccacac caatggtcaa taccataagg agcaggaggc tgccgcttaa tcttacctgc
      721 cgaaatctcg ccatcttaag acccccttcc tatgattgtg tgttcatggt tccgtagcag
      781 gtgtagcgtg gccccataac gtgtgaattg ccgatgtgtt acaggattgt taaaaatctc
      841 tacaggccag gtttcacaaa tctgaaagat gagacggaga cggcctctga actaagaact
      901 ttcaagaaca gttaacgtgg aattaacact gccgtaatcg aatcgaaagc gatcataggt
      961 actgtcgctg ggacaatccg aacaaagaaa atgcattccg gctggaataa ggcagtacag
     1021 ttaggagaat aagtatgcga gcgactgtga ggcagatacg attgtttatg ctcacgacat
     1081 gtctttttgc gtggccggtt gctccgacac agtccgaaac cgcgctgctg gtcaaggtgg
     1141 acggatcaag cactgtcttt cctattactg aagcggttgc agaagaattc caaaaagaca
     1201 accacgggac aatcagggtc accgtgggga tttctggtac tgggggagga ttcaagaagt
     1261 tctgccgagg ggaaaccgac gtgcaagatg cttctcgccc gatctctgcg agtgagatgg
     1321 aggcctgtcg aacgaacgga attcagtatt acgaattacc cgtggctttc gatgccctca
     1381 ccatcgcagt gagccctcaa gccacatgga ttgatgccat cacggtagcc gaattgaaac
     1441 agatgtggga accctctgcg cagggacggg tgatgaagtg gaatcaggtt cggtcgacct
     1501 ggcccgacag tccgctgaaa ttattcggcg ccgggtcaga ttctggtacg ttcgactact
     1561 ttaccgaagc cgtgacggga aaggccaaag cgagccgcgg tgattacacc gccagcgaag
     1621 atgacaacac gctcgtgcag ggaatctcca acgataagca ggcgttgggt tatattccgt
     1681 acgcatactt cgagccgaat aagatgcgct tgaaagcagt cgcggtcgat ggtggacgtg
     1741 gtccggtatt accctctcga gagacggtgg agaatggcgc ctaccaacct ctgtcccgcc
     1801 cgatgtttat ttatgtgagt agcaagtccg ccgggaggcc cgatgtgaaa cgattcgtcg
     1861 agttctatct ctcgcaggcg ccgacgctgg ttccacaggt gaagtatgtt cccctgcctg
     1921 cacaggctta tgatctggcc ctgacacatt ttcgtaacgg caaactggga accgcatttc
     1981 aaggcatttc aacgatcggc ttgaaaattg aagatctctt gcgccgagag gcgaaactgt
     2041 agccgagtga gcgaatggat acgcaacttg ttggatcaga ggtcttgaga aaggcagcgg
     2101 actttcgcat cttctctcgg acattgttcc atcttaaaga acgggggatc gagctgcttt
     2161 tgttcttggc ggcgctcacg tctgttgcga ttacggtcgg tattgtcggg gtgttagtct
     2221 acgaatcatt gacattcttt cgagacgtct cgttgttcga tttcctgacg gataggcaat
     2281 ggactcctct cttttcagag ccgcactacg gcattatgtc gttagtgtct ggaaccttag
     2341 tcaccacttc cattgcactc ttggttgcaa ttccgatggg tagtctggtt gctatctacc
     2401 tgagcgaata tgcacggccg aatgttcgag agattgtaaa accagcactg gaattattga
     2461 gtgccgtgcc gacggtcgtc tacggatatt ttgcccttct cttcgtcact ccggcactac
     2521 agaaggtttg gccagggctg ccgggcttta acatgctcag cgccggattg gtgattggaa
     2581 tcatgatcgt gccctacgtg agttccgtca gcgaggatgc gatgcgggca gtcccagtct
     2641 atttgcggga gggagcctat gccttaggcg cgacacgcat gcagacagcc atccgggtgg
     2701 tttttccatc cgctctttcg ggaatcgctg ctgcctatgt cctgggggta tcccgtgcaa
     2761 tcggtgaaac gatggtcgtg gccattgcgg ctggaatgca gccgaccctg acctggaatc
     2821 cccttgaacc ggccgccacg atgaccgcct acatcgtgca ggtcagcctc ggtgatttgc
     2881 ctcacggaag cgtcggttat caaaccattt ttgcgactgg cttgactctc ttactgatga
     2941 cgctcctctt caacatcgcc ggccatgttc tcagaaaacg atatcgacaa gcctactagt
     3001 cctggtttgc tcaaagccgc gattgatcgg cgcaagcgaa ctgatctgct ctttgcggcg
     3061 gtgggactct ccacgatgct gcttgcgctc gtgaccttgc tggccttgct tggtcaattg
     3121 ggcatggacg ggatcggtcg cctctcgttg caatttctca cctcctttcc gtccagattc
     3181 ccagcacagg ccggcatctt gagcgcctgg gttgggacta tcctcgtgat ggtcctcacg
     3241 gctctcacag cagtaccgtt agggcttggg gcggcgatct atcttgaaga gtatgctcca
     3301 cgcaattggg tcaccgatct catcgagatc aacattgcga atctcgcggg agtaccctct
     3361 atcgtgtatg gcctgatggc acttggactc tttgtctatc agtttcattt tggccagagt
     3421 ttcctcaccg ctgggttgac tctaggcatg ctcattttgc caatggtgat cattgcaacg
     3481 cgggaagcca tccgaagtgt tccgcctcaa atccgtgaag cggcgtatgc ccttggcgca
     3541 accaagtggc agacagttag ggatcacgtg gtgccctatt caatgggtgg aattcttacg
     3601 gggctgatct tggcattatc acgcgctgtt ggtgagacgg ctcctttgat tacggtcggc
     3661 gccctgtcgt ttatcgcctt tcttcctcac ccaccgtggc agccggattt cccctttttc
     3721 tctttccatt ggctgttcga tccctttacg gtcatgccca ttcagatgtt caactgggtg
     3781 tctcggccgc aggaggactt ccacgcgaac gccgcagcag ccggcatcat cctcttgctg
     3841 atgacattag ccatgaatgc gctggccatt tcactccgtg cacgatttcg aaagcgaatc
     3901 cactggtgaa caggtgacaa caagctccga aacccggctg tcgctcaaag ccgaagcaaa
     3961 agggctcagc ttctattatg gggcggtgcg cgcactcaac gacatctccc ttggtttagt
     4021 cgatcgtcga gccacggcgt taattggtcc gtcaggctgt ggaaaaacca cctatctacg
     4081 gtgcctcaac cgcatgcacg atctctatgc gggcacccgc tacgagggag aagtgctcct
     4141 ctatcccgac caggtgaata tcgtcgatca atctgtcgat cctatcgaga tccgcatgcg
     4201 tgtggggatg gtttttcaaa aacccaaccc ttttccaaaa tccatctatg aaaatgtcgc
     4261 gtatgggctt cgcattcgac aagccacaaa acgaagcgag ttgaacgagg ctgtcgaacg
     4321 tgccttgcgc gacgcggctt tatggacaga ggtgaaagat cgcctgcacg atctcgcgtc
     4381 aaatctttcg ggcggtcagc aacagcgcct ctgtattgcc cgtgccctcg caactggtcc
     4441 ggaaatgctc ttattcgacg agccgacgtc ggctctggat cctaccgcca ccgcacggat
     4501 cgaggaactc gtcaccgaac tgaagcagag ggtaaccatc gtcatcgtga cccacaacat
     4561 gcaacaggcc gcacgggttt cagactttac cgctttcatg tacgaaggca agctcgtaga
     4621 attcgatcga acggagacca tcttcacgaa tccatccaat aaactaactg aagactacgt
     4681 caccggccgg tttgggtaac ggaagcctga gcgaggatgc caatgcaacg acatatcgat
     4741 caagagttag ccgagctgaa gacgcagatc ctccggatgg gaggcctggt ggaagggcag
     4801 attgaacggg ccatccgtgc cttggtggaa cgagacgtcg atctggcttc acaggtcatc
     4861 gatcgagata cattggtcaa tagcctcgac gtggagattg atgaaaactg tatacggttg
     4921 ctcgcccttc aggcgcccgc ggcaggcgat cttcgatttg tcactacagc gatgaaaatc
     4981 tcgaccgaac tcgaacggat gagcgaccta gcggaaatca tttgtgagcg tgtgattgaa
     5041 ctcaacgaag aaccacagtt gaaaccctac atcgatattc ctcgcatggc aaactggacc
     5101 atgcgaatgg tgcgtgaatg tctcgatgcc tttgttcggt ctgactcagc tctggcgaga
     5161 aaggtatgca ccgatgatga tttcgtcgac aatctcacgc accagctatt tcgggaactc
     5221 ctgtccttca tgatcgaaaa tccaggaacc atttcacggg ccattcgtct tacatttatc
     5281 gggaaatata tcgaacggat cgccgaccac gccacgaacg ttgccgaact tgtcgtctat
     5341 atggtggaag ggaaaatcgt ccgccacatc gatccacccg ctctcctgga taagaattcg
     5401 tgaggtgcct catatcaaaa ccggctgact cattctacac accacttacg tgaaccatct
     5461 agaaggagga acaggacaca gagtcttgac aaggaatgtg aagaggtaga cgggtaacga
     5521 cagaaccctt cctactcgta ctgcgacatc ggatccagaa cggcctgcta tggttgttcg
     5581 atcagtctcc agccctcttt ttgaacgcat cgttcagtcg cgttcagaac catcagctgg
     5641 ttaccctgct ggagcttagg atctcgcaag accatacttt cgcaccggtt gtagtcagtc
     5701 gcgaattccc cgctcggctt gtttggatga acccactccg tggaggaaca agctgacaaa
     5761 aggctacaag ttgcaactaa cgaccccaga attctaatgc gcatgaattc ctcctctaag
     5821 actcctcgcg aaagataaac tgtccaatta gagggcgtca agcggcatat aagcaagaat
     5881 gaggtgtatg aagagactac acgactccta ccgaagccgg atacaatctc agataaactg
     5941 aagagcgcat ctacggatag aaaaatccac catatcaatt gcttacgagt tatatgtgaa
     6001 taaattcatg aaccactcct ttgtatggtt gtgcagggaa aagcaactct gctaccgtgc
     6061 acaactacag ggagggtttc gtgacaccga tcgaccatct gatgatggat gccaagcaag
     6121 caatcatgga ggagcaacac cggcgattcc aggatctcca tcgagaaggg cggtgggtcg
     6181 aagccctgca gcagatgcat gtgacattga gttgtgcgtc agatctcctg aacgagtcgc
     6241 tgcaagcatt ggacaaagca cttggcagtt gccacgctcc ctcct
//
LOCUS       DATDNW010000021         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_685160, whole genome shotgun sequence.
ACCESSION   DATDNW010000021 DATDNW010000000
VERSION     DATDNW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685160"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1182
                     /gene="hemW"
                     /locus_tag="VI359_00540"
     CDS             46..1182
                     /gene="hemW"
                     /locus_tag="VI359_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY6972766.1"
                     /translation="MSRTPLGLYLHIPFCRQRCDFCAFYLEIYRDGRARTFVDSLRRE
                     IRFYATQDLAATHSIQSIYLGGGTPTTLSPDQLESILTDIRQTFVVLPDAEVSIEAHP
                     STVSEQSLTRLIKAGFNRISFGAESMDDQEFLSIGRPGTSQDTVRAVAIARAAGFTNI
                     NLDLMYGLPGQTVANWQQSLDQVVQLDPAHISCYALTIEEETKLAQNIERQVTAAPDD
                     ARQVEMDDAADLLLRRAGFERYEISNYAKPGYRCRHNLLYWTDGAYLGLGPSAQSYVN
                     GSRFGNIADVMAYSSTLSEGRLPVQAYTELSDRERLRDAVVFGLRLVQGISTQALEAH
                     ALHYGHRETLMGLRAQQLIEEEETRSRLTAKGRQYADTVAERLF"
     gene            complement(1194..1553)
                     /locus_tag="VI359_00545"
     CDS             complement(1194..1553)
                     /locus_tag="VI359_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972767.1"
                     /translation="MLNAKVWWPLFPLLLLVVIVCLTWALVIVIRRGTDAKARWLQIG
                     AFSCYALAAASAIASERGLVSANLHRPFSLFTQVCLLLALMHHWRSGNRSLRWLNGVA
                     WAGILSDAALHVLMVRH"
     gene            1666..1875
                     /locus_tag="VI359_00550"
     CDS             1666..1875
                     /locus_tag="VI359_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972768.1"
                     /translation="MPVNMDPTKRRRKSQAAPKAVATPTTDSVKADSPKKFGDETEAD
                     RLKAMAEAESKNLWDATEVIDMDKW"
     gene            complement(1880..>2351)
                     /locus_tag="VI359_00555"
     CDS             complement(1880..>2351)
                     /locus_tag="VI359_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY6972769.1"
                     /translation="ETRWTRNTSIVVLTGATLLVAVMSEILVGALEPAAQRLGMTQLF
                     VGLILVALVGNAAEHSTAVMVALKNKMDLAFGIAVGSSLQIALLIAPILVFASYAFGV
                     PLDLIFTPFEVAAVTISVVIVGFVAVDGESHWMEGVMLVGVYLMLAIAFFFLPA"
BASE COUNT          596 a          689 c          611 g          455 t
ORIGIN      
        1 gctgtccaga gacttctcca aaccgcaatc atttaatctc taaacatgtc gcgcacgccg
       61 cttggtcttt acctccacat tccgttttgt cgtcagcgat gcgatttctg cgccttctat
      121 ctggaaatct acagggacgg tcgcgcccgg acatttgtag attccctgcg gcgcgagatc
      181 cgtttctacg ccacacagga ccttgcagcg actcattcga ttcaatccat ctacctcggt
      241 ggcggaacac ccacgacact ctcccccgat caattagaaa gtattctgac cgatatccgg
      301 cagacattcg tcgttttgcc cgatgcggaa gtatcgatcg aggcccatcc ttcgacagtc
      361 tcggagcaga gtctcacccg cttgatcaag gcaggattca accggattag tttcggcgcg
      421 gaatccatgg atgaccagga atttctttca atcgggcgac ctgggacatc ccaagataca
      481 gtccgtgcag ttgcaatagc ccgtgccgca ggatttacaa acatcaatct tgatctcatg
      541 tacggtttac ccggtcaaac cgtggcgaac tggcaacaat ctctcgatca ggttgttcag
      601 ctcgatcccg cccatatttc ctgttatgcg ttgacgatcg aagaagagac gaagctggct
      661 cagaacatcg agcgacaggt cactgcggcc cctgatgacg cacgacaagt cgagatggac
      721 gacgctgccg atctgttact ccgccgagcc ggttttgaac gatatgagat ttccaactat
      781 gcgaagccgg gttatcgctg caggcacaat cttctgtatt ggaccgatgg agcctatctt
      841 ggcctcggtc cgagcgccca atcgtatgtc aatggaagcc gcttcggaaa catcgctgat
      901 gtgatggcgt acagcagcac attgagcgaa ggccgtctgc cggtccaagc ctatacggag
      961 ttgtccgacc gtgaacggct tcgggatgcc gtcgtctttg gactacgtct ggtccagggg
     1021 atttccacac aagcgctcga agcacacgcc ttgcactatg gccatcgcga aacgctgatg
     1081 ggactccgag cacaacagct aatcgaagaa gaagaaacgc ggagcagact cacggccaag
     1141 ggacggcagt atgctgatac cgtcgcagag aggctgttct agaactgcac ctgttagtgg
     1201 cggaccatga gcacatgaag tgccgcatcg gaaaggatcc ccgcccacgc gacaccattt
     1261 aaccatcgaa gggaacgatt tcccgatctc caatgatgca tcagcgcgag cagcaggcaa
     1321 acttgcgtga acaaggaaaa gggccggtgg aggttcgccg aaactaagcc acgctcactg
     1381 gcgatggccg acgcggctgc cagagcatag caactgaatg cgccaatctg gagccatcgt
     1441 gctttcgcat cagtaccccg tcgaatcacg ataacgagcg cccacgtcag acacacaatc
     1501 acgaccagca gtaacagagg gaacagcggc caccagactt tcgcattcag catagggcga
     1561 ggactctaac aatcgaccgg cgttgcgtca atgaccggct tcccccgaca ttgacattct
     1621 taccaatcag ggtggaaact actttcgtcc tgtatggagg ttcggatgcc tgtcaatatg
     1681 gaccccacca aacggagacg gaaatcccag gctgctccca aagcagtggc cactcccacg
     1741 accgattccg tcaaagcaga ttcgccaaag aagttcggcg acgaaacaga ggccgatcgc
     1801 ctcaaggcga tggcagaagc tgagtcgaag aacttgtggg atgcgaccga agtcatcgac
     1861 atggacaagt ggtgacccgt caagcgggca aaaagaagaa ggcgatcgca agcatcaggt
     1921 agaccccgac cagcatcacc ccttccatcc agtgcgactc gccatctacc gcgacgaacc
     1981 cgacaatcac gacagagatg gtcaccgcgg cgacctcgaa gggtgtaaag atgagatcga
     2041 gcggcacccc gaacgcatag cttgcaaaga ccaggatggg agcaatcagc aaggcgattt
     2101 ggagactcga gcccaccgca atcccaaacg ccaaatccat cttgtttttt agcgcgacca
     2161 tgacggcggt cgaatgttct gctgcattcc ccaccaatgc aaccagaatc aacccaacaa
     2221 acagctgcgt cattcccaaa cgctgggcag cgggttccag agcaccgaca aggatttcac
     2281 tcatgaccgc caccaacaga gtcgcgccgg tcaggacgac gatcgaggtg ttgcgggtcc
     2341 aacgcgtttc t
//
LOCUS       DATDNW010000022         4297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_616787, whole genome shotgun sequence.
ACCESSION   DATDNW010000022 DATDNW010000000
VERSION     DATDNW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616787"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VI359_00560"
     CDS             <1..352
                     /locus_tag="VI359_00560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY6972770.1"
                     /translation="MLSLLPQYTTDEFDSRHRLVLVAAQRAKHLLQGSRSHGTSRFTK
                     ETTIALDEVLRGETKYLVGKEARDAMKEAKRGKEGETERIAMMSGDDAREIKKELSNY
                     VDDTAKPTGAEGEE"
     gene            356..1603
                     /gene="coaBC"
                     /locus_tag="VI359_00565"
     CDS             356..1603
                     /gene="coaBC"
                     /locus_tag="VI359_00565"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY6972771.1"
                     /translation="MEGQRQPTLVGARIILGVTGSIAAYKAVGLLRALVKEGATVEVV
                     MTHAATKFVAPLTFEVLSGGAVSLDLFEAHHEMKHLSMPEQADLIVVAPATANFLAKA
                     ASGLADDLLSTMLLTAHCPLLVAPAMDGGMWSHSAVAQNVQTLRARGVIVLDPDEGPL
                     ASGRFGQGRLADESRIVEAIETALFPQRDWQGHRILVSAGPTQEPIDPVRFISNRSSG
                     KMGYALAEAARARGAQVTLVSGPTALPAPAGVEIVEVKTAEEMAKAMCSRLSWSTVVV
                     MAAAVADYRPAETSVHKLKKQEGRGEQALRLEPTSDILATLAAQRTNQLLIGFAAETQ
                     NLVTHARRKLSAKGLDMIVANDVTREGAGFGSDQNAATVIDRQGRTTEFPLMAKRQLA
                     DQILNVARTLILPRGAQTGIRTE"
     gene            1620..2396
                     /locus_tag="VI359_00570"
     CDS             1620..2396
                     /locus_tag="VI359_00570"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972772.1"
                     /translation="MAPSKKSSVNIQEIDRLALQLVKDPHSKVFMPLAEEYVKAGMWQ
                     EAAAVLEDGLKYYPGFITAMVALGRAYDQMGQPTKAKAILEEAIKLSPENLRAHRTLV
                     KIYSAQGLADAALKSCTVILAVNPQDEEALSIQAQLGGTPTSQTSPAKTSAPASKAVK
                     SVPETPAPQASASISSKSTKPDAVDPLTAATVLMETTLERIRRPDVESTEEAIDPAPT
                     NSASTIAPSPRVSQLESWLRSIQLRRRDLDQADHAQSASS"
     gene            2516..3016
                     /gene="aroQ"
                     /locus_tag="VI359_00575"
     CDS             2516..3016
                     /gene="aroQ"
                     /locus_tag="VI359_00575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY6972773.1"
                     /translation="MIRILILHGPNLNLLGSREQSVYGTAPLDEVDAAISKLAEELAV
                     EVDIRQSNSEGELVTWIQEARSGYQGIVINPAGYTHTSIAIRDALAAVNLPTIEVHLS
                     NIHRREEFRHRSYVAGVATGQIAGLGPTGYLLALRGLHDYLTAPRRQKKAAATSPTRR
                     AVRNTR"
     gene            3065..3625
                     /gene="efp"
                     /locus_tag="VI359_00580"
     CDS             3065..3625
                     /gene="efp"
                     /locus_tag="VI359_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY6972774.1"
                     /translation="MISTTDFRGGVRLMVDGEPFYIVEFQHVKPGKGGAFVRTKLKSY
                     ISGNVLDRTFRSGERFDEPDLEEREMQFLYATGNSYTFMDTETYEQLTFEKSQLGENA
                     DLLKENMTAKILIYEHRPIAVELPIFIELKVVDADPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIKIDTRTRAYVERVR"
     gene            3622..4194
                     /gene="accB"
                     /locus_tag="VI359_00585"
     CDS             3622..4194
                     /gene="accB"
                     /locus_tag="VI359_00585"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY6972775.1"
                     /translation="MSKKRSRKQRANKAPIILPQDFADTPVGQGKSFLLGNQSRHVQE
                     LIDILKRNNLTELELEREGVRIRVRHEQGIKTITSTVADASSGTVASASQPSPTGSTS
                     AEDVTGLVTIASPIVGTFYRSPSPDADPYVEEGDYVKKGQVLCIVEAMKLMNEIESEV
                     DGRVTKILAESTKPVEYGQALFLVDPKATP"
     gene            4228..>4297
                     /locus_tag="VI359_00590"
     CDS             4228..>4297
                     /locus_tag="VI359_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY6972776.1"
                     /translation="MFKKVLVANRGEIALRVIRACKE"
BASE COUNT         1049 a         1172 c         1225 g          851 t
ORIGIN      
        1 tatgttaagc cttctcccac aatacacgac ggatgaattc gattcgcgtc accggctggt
       61 gctggtggca gcccaacgcg ccaagcatct cttgcaggga tcgcggtctc acgggacttc
      121 ccgcttcacg aaagaaacca caatcgccct cgacgaagtt ttgcgtggag aaacgaaata
      181 cctggttggg aaagaggcgc gtgatgccat gaaggaggct aaacgcggca aggaaggaga
      241 aacggaacgg atcgcgatga tgagcggcga tgatgcccgc gaaatcaaga aggaactgag
      301 caactacgtc gacgacacgg cgaaaccgac cggagccgaa ggtgaggagt aacaggtgga
      361 gggccagcga cagccgacat tagtcggcgc gaggatcatt ctcggggtca ccggcagtat
      421 cgcggcgtac aaggctgttg ggctcctacg cgcgctggta aaggagggcg ccaccgttga
      481 agtggtcatg acccatgccg ctactaaatt tgttgccccg ctgaccttcg aggtgctgtc
      541 ggggggggcg gtttcactcg atctgtttga agcccaccac gagatgaagc atctgtcgat
      601 gcccgaacag gccgatctca ttgtcgtggc accggcgacc gcgaactttc tggccaaggc
      661 ggcgtccggg ttggcggacg atcttctttc aacgatgttg ctgaccgctc attgcccgct
      721 cctggtcgca ccggcgatgg atggaggaat gtggagccat agtgcggtcg cacagaacgt
      781 ccagactcta cgggcacgag gagtgattgt cttggatcca gacgaagggc cgttggcttc
      841 gggtcgattc ggtcagggtc ggttggccga tgaatcgcgc attgtggagg ccattgagac
      901 cgccttgttt ccgcaacggg actggcaagg acatcggatt ctcgtttcgg ccggtccgac
      961 gcaagaaccg atcgatccgg tgcgatttat ctcgaatcgc tcttccggga aaatggggta
     1021 tgccttggca gaagccgctc gggcacgggg cgcacaggtg accctcgtat caggtcctac
     1081 ggcccttcca gccccggcgg gtgttgaaat tgtagaggtc aagacggccg aagagatggc
     1141 aaaagcgatg tgcagtagac tttcctggtc gaccgtcgtc gtcatggcgg ctgctgtggc
     1201 tgattatcgt cccgcagaga cttccgtgca caagctcaag aagcaggagg gacggggaga
     1261 gcaggcgcta cgcctggaac cgaccagcga cattcttgcc accttagccg cccagcggac
     1321 gaaccagctg ttgattggat ttgccgctga aacgcaaaac cttgttaccc atgcgagacg
     1381 gaagctctcc gcaaagggtt tggacatgat tgtggccaat gatgtcacca gagaaggagc
     1441 agggtttgga agcgaccaga acgccgcgac cgtgatcgat cgccaagggc gaaccaccga
     1501 attcccttta atggccaagc gccagcttgc cgatcagatt ctgaacgtcg ctcgcacgct
     1561 gattctacct cgcggtgctc aaaccggcat tcggaccgag tgagaaaggc aggctcacga
     1621 tggcgcccag caaaaaatct tccgtaaaca tccaggaaat tgaccggttg gccctccagt
     1681 tggtgaaaga cccccattca aaagtgttca tgccgcttgc agaagagtat gtcaaagccg
     1741 ggatgtggca agaggcggca gccgtcctgg aagacggcct gaaatactat ccaggattca
     1801 tcacggccat ggtggccctt ggtcgtgcct acgaccaaat gggacagccc acgaaggcaa
     1861 aggccattct ggaagaagcc atcaaactca gcccagagaa cttgcgggcg caccgaaccc
     1921 tggtgaaaat ttattctgcg cagggactcg ccgatgctgc attaaagtca tgtaccgtca
     1981 tcctggcggt gaatccacag gacgaagaag ccttgtcgat tcaggcgcaa cttggaggaa
     2041 ctcccacttc acagacatca cctgcgaaga cctcggcccc tgctagcaaa gctgtgaaat
     2101 cggtgcccga gactcccgca cctcaggctt ccgcctcgat cagctcaaag tcaactaagc
     2161 ctgatgcggt ggatccctta actgctgcaa ccgttctgat ggaaaccacg ctcgagcgga
     2221 tccgccgtcc ggacgtggaa tccaccgagg aggcgatcga ccccgctccc acgaactctg
     2281 catcgacgat tgcaccgtca cctcgcgtat ctcaactcga atcctggctc cgctccattc
     2341 agttacggcg gcgcgatctc gaccaagccg atcatgcgca gtcagcgagt tcataagaaa
     2401 ttccaatagt ttgaccggcc tggagcaccc tgttacaatg cctctccgct ggtcgttccc
     2461 gcacaaccac gggaagtcgc tctaccaatg attatcgaca tgatctgacg tggatatgat
     2521 acggattctg atcttgcacg ggcccaattt aaatctcttg gggtcccgcg aacagtcggt
     2581 ttatggtacc gccccgcttg atgaagtaga tgcggcaatc tcgaaactgg ccgaggaact
     2641 agcggtcgaa gtcgacattc ggcaatcgaa cagcgaaggg gaactcgtga cctggattca
     2701 ggaagcgcgg agcggatacc aggggatcgt catcaaccca gccggatata cccataccag
     2761 tattgccatc cgggacgctc tggccgccgt caatctgccc acgatcgagg ttcatctgtc
     2821 aaacatccat cggcgcgaag agtttcgcca ccgatcctac gttgcagggg tcgcgacagg
     2881 gcagattgcc ggccttggac cgaccggtta tttgcttgcg cttcgagggc tgcacgatta
     2941 cctgactgcg ccgcgtcggc agaaaaaagc tgctgcgacg tcacccactc gacgtgccgt
     3001 gcgcaacaca cgataaccca gataggtttc agactgagtc tttcagacga gaagggagta
     3061 cggagtgatt tctacgacgg attttcgggg aggtgtccgg ttgatggtcg acggcgaacc
     3121 gttttacatc gttgagtttc agcatgtgaa accgggcaag ggcggcgcct tcgtgcgcac
     3181 gaaactcaag agctacattt ccggcaacgt actcgaccgc acgttccgat cgggcgagcg
     3241 attcgacgaa ccggatcttg aagaacgtga gatgcaattt ctctatgcga ccgggaattc
     3301 ctatacgttt atggatacag agacctatga acaactcacc ttcgagaaaa gccagttggg
     3361 agagaacgcg gatcttttga aggaaaacat gaccgccaag atcttgattt atgaacatcg
     3421 cccgatcgca gtcgaactcc cgatctttat cgaactgaaa gtcgtggatg ctgatccagg
     3481 tgtgcggggt gacacagcgt cgggtggaac caagccggcg gtggtggaga ctggagccac
     3541 aattaaggtc ccactgtatc tggaagtcgg cacagtaatt aagatcgata ctcgtacaag
     3601 agcttacgtg gagcgcgttc ggtgagtaaa aaaaggtctc gcaagcagcg agcaaacaag
     3661 gcgccaatta ttcttccgca agattttgct gacaccccag tcgggcaagg aaagtccttt
     3721 ctgctgggta atcaatccag gcacgtccag gaacttatcg acatattaaa gcgaaacaat
     3781 ttgaccgagt tggaactcga acgagaagga gtcaggattc gtgtccgaca cgaacaaggg
     3841 ataaaaacaa ttacgtccac cgtcgccgac gcaagctccg gtaccgtcgc gtcagcatct
     3901 caaccaagcc ccacagggag cacatcggcc gaagacgtaa ctggcctagt aacgattgcg
     3961 tctcccatcg tcggaacatt ttaccgttcc ccctccccag atgccgaccc ttacgtagaa
     4021 gaaggggatt acgtgaaaaa aggtcaggta ctgtgtattg tggaagctat gaaactgatg
     4081 aatgaaattg aatcggaggt ggacgggcga gtgacaaaaa ttctggccga aagcacaaag
     4141 ccggtcgaat atggtcaggc gctcttcctc gtcgatccga aagccacacc ctaattcgcc
     4201 ttatctaatc caactcggag ctacagcgtg ttcaagaaag ttctcgtcgc caatcgagga
     4261 gaaatcgcac tacgggtcat ccgggcctgt aaagaac
//
LOCUS       DATDNW010000023         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1163389, whole genome shotgun sequence.
ACCESSION   DATDNW010000023 DATDNW010000000
VERSION     DATDNW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163389"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="hemC"
                     /locus_tag="VI359_00595"
     CDS             complement(<1..531)
                     /gene="hemC"
                     /locus_tag="VI359_00595"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY6972777.1"
                     /translation="MAASIPERSTIVLGTRGSKLAVQQSEWVQARLRELAPEVTVTLR
                     RIQTSGDKILDVPLANIGGKGLFVKEIEEALLAGEIDLAVHSMKDVPTQLPSGLAILC
                     VPQREDPRDALISRTGCVFKDLPQGAKVGTSSLRRQAQFLHARPDLKIDMLRGNLDTR
                     LRKLREGHYDAIVLAVA"
     gene            complement(541..900)
                     /locus_tag="VI359_00600"
     CDS             complement(541..900)
                     /locus_tag="VI359_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972778.1"
                     /translation="MRGMLTRLWGNIGAREDRAPKLFTCPKCRATYLGHEPLPDCPRC
                     GFDYRGQERFRWDVLVYLLSILGLISFLLVSSSYRSILGDPSADRVRLSGGEGAEKLP
                     GADRPATFRTPYHEPAR"
     gene            complement(906..2309)
                     /gene="hemA"
                     /locus_tag="VI359_00605"
     CDS             complement(906..2309)
                     /gene="hemA"
                     /locus_tag="VI359_00605"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY6972779.1"
                     /translation="MHIVVVGLSHKTAPVEVREKLAVPESRLGEALTRLCSYAGVREG
                     LLLSTCNRVEVYAVVDELDAGYGRIQEFLADAHFSLSSEQLTPHLYWHTGDRAIGHLF
                     RVAASLDSMIIGESQILGQLKDAFEVALNHKTSGVILNKVVKKAISVAKRVRTDTKIA
                     EMAVSVSYAAVELAKKIFSDLSEKTVLLVGAGEMAKLAARHFVANGVRHVRVTTRNPH
                     QALELADKFNGTAVPFELFREDMASADIVLVSTGAAHYLIGSDDVHRAVKQRMNRPMF
                     FIDISVPRNIDPAVRHVDNAFLFDIDDLKLRVEQNREDRLREAEKAEQMVTEEVGVLR
                     QWFQSLEVTPTIVALKNRVDDIKRAELEKALGRLAHLSVQERELVEAMASAIVNKLIH
                     STLVTLKTEVNSTDGVAFVEAARRFFNLGENSPSSQNGLSSSSGGASDRGAADDESQI
                     EGSEPPSVVQNPRRAGS"
     gene            complement(2311..3114)
                     /gene="ccsA"
                     /locus_tag="VI359_00610"
     CDS             complement(2311..3114)
                     /gene="ccsA"
                     /locus_tag="VI359_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY6972780.1"
                     /translation="MAAVFFMVTMALYFGATVLFLAYLLRRAEALSTVSLGITAAGFC
                     THTVAIVARMMGATGSSTPTFHEALSFFSWMLVLVFLAVELRHRIHVLGSFIVPLALV
                     SLVSAAALPDTAPTLRPMFGTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKRF
                     NVLYSKLPALDFLDHLNQQSIVMGFPLLTLGIITGAISAEFGQGSYVSWNPEQTWALV
                     TWLFYFAVLVGRLTVGWRAKRAAYLTIIGFAGVILTLVGVVLKSYGPVS"
BASE COUNT          752 a          945 c          824 g          639 t
ORIGIN      
        1 cgcgactgcc agcacaatgg catcgtagtg gccttcccgc agtttcctga gtcgcgtatc
       61 caaattgccc cgcagcatat cgatcttcaa gtccggcctt gcatgaagga attgcgcctg
      121 gcgtctcaga ctactggttc cgacttttgc cccctgggga agatccttaa agacgcatcc
      181 ggtacgactg atcagagcat cgcggggatc ttcacgctga ggaacgcaga ggatcgccag
      241 cccgctcggt aattgcgtcg gtacatcctt catgctatga acggcaagat cgatttcacc
      301 ggccaacagt gcctcttcga tctccttgac gaatagtccc ttgcctccaa tattggccag
      361 cggaacatcg agaatcttat ctccggacgt ttgaatccgc cgcaaggtca ccgtgacctc
      421 gggcgcaagt tctctcagtc tggcctgcac ccattcgctt tgctgcaccg ccagtttgct
      481 gcctctcgtc ccgagaacaa tggtggaacg ctcagggatc gaagcggcca ttgattcctt
      541 tcatcgagcc ggctcgtgat acggggtacg aaacgtcgca ggtcggtccg ctcccggcag
      601 tttttctgct ccctcgccgc ccgacaatcg cacgcgatca gccgaagggt cccccaagat
      661 gctgcgatag gacgacgata ccaggagaaa gctgatgagt ccgaggatcg acagcaggta
      721 cacgagcaca tcccaacgaa accgttcttg ccctcgatag tcgaatccac accgcgggca
      781 atccggaaga ggctcgtggc cgaggtacgt cgcccgacat ttgggacaag tgaacaactt
      841 cggcgcgcgg tcttctcgtg ccccgatgtt cccccacaga cgtgtgagca ttcctcgcat
      901 acaattcagc tccccgccct ccgagggttc tggacaactg atggaggctc cgacccttca
      961 atctgcgatt catcgtcagc cgccccacgg tctgacgcac cgcccgacga agaagagagc
     1021 ccattttgac tcgaaggtga gttctcaccc agattaaaaa accgtctagc ggcttccacg
     1081 aaggcaaccc cgtctgttga attgacctca gtcttcagcg ttacgagagt gctgtgaatc
     1141 agcttgttca cgatcgccga ggccatggcc tcgaccaatt cacgctcctg gaccgaaaga
     1201 tgggcaagcc gtcccagcgc cttttccaat tcagcccgct tgatgtcatc gacacgattc
     1261 ttcaacgcga caattgtggg cgtcacctcg agcgattgaa accactgccg gagaactccc
     1321 acttcctcgg tcaccatttg ttcggccttt tccgcttcgc gcaatcgatc ttcccgattt
     1381 tgttcgactc tgagcttgag atcgtcgatg tcaaacaaga acgcgttgtc gacgtgccga
     1441 accgccgggt cgatgttgcg tggaaccgag atatcgataa agaacatcgg acgattcatg
     1501 cgttgtttga ccgcccgatg cacgtcgtcg ctgccgatga gataatgcgc cgctccagtc
     1561 gacacgagca cgatatccgc cgacgccatg tcttctcgaa atagctcaaa cggcacagca
     1621 gtgccattaa acttgtcggc caattccagc gcttggtggg ggttccgcgt cgtcacccgg
     1681 acgtgccgaa cgccgttggc cacaaaatgt ctcgcagcca acttggccat ttcaccggcg
     1741 ccaaccaaga gcacggtttt ctcgctcaga tcggaaaata ttttctttgc caactcaacg
     1801 gcggcgtaac taaccgacac cgccatctcc gcaatcttcg tatcggtccg gactcgtttc
     1861 gcaacggaaa tggctttctt gacgaccttg ttcaaaatca cgccactcgt cttatggttc
     1921 aaagccacct cgaacgcatc cttcaactgt ccgaggattt gagattcacc gatgatcatg
     1981 gaatcgagac tcgcagcgac gcggaacaaa tgcccgattg cacgatcccc cgtatgccag
     2041 tacagatgcg gcgtcagttg ctcggaggaa agagagaaat gagcatccgc cagaaattcc
     2101 tggatgcggc cgtagcctgc atcgagttcg tcgaccacgg catacacttc gacacgattg
     2161 cacgtcgaaa gcagaaggcc ctctcgcact ccggcatagg agcagagcct ggtcaaggcc
     2221 tcgcccaatc ggctttctgg aacggccagt ttttcccgca cttccacggg agctgtttta
     2281 tggctgagcc caacgacgac gatgtgcatg tcaagacacc ggtccgtaac tcttcagcac
     2341 gacgccgacg agtgtaagaa tgactcctgc aaatcctata attgtcagat aggcagcccg
     2401 ctttgcgcgc caacccaccg tcagccgtcc aaccagcacg gcaaagtaaa acagccaggt
     2461 gacgagggcc cacgtttgtt caggattcca gctcacgtag gaaccttggc caaattcggc
     2521 tgaaatcgcg ccggtgatga tgccgagcgt gagcagggga aaccccataa caatcgattg
     2581 ctgattcaaa tggtccagaa aatccaatgc aggaagtttt gaatagagca cgttgaatcg
     2641 tttggacttg agaagtccat cctgaatcag atacatcaca ccagccacga atgccacggc
     2701 aaatccgacc gttcccaaca tgctcaaggt cacatgaacc cacaaggtgc caaacatcgg
     2761 cctgagcgtg ggggcggtat cagggagcgc cgcagccgag acaagggaaa cgagagcgag
     2821 cgggacgata aaggagccga ggacgtgaat ccggtggcgg agttctacgg cgagaaacac
     2881 aagaacgagc atccacgaga aaaatgagag ggcttcgtgg aacgtcggcg ttgaacttcc
     2941 tgtcgctccc atcatccgcg ccacaatggc gacagtgtgg gtgcagaaac cggctgcggt
     3001 gatgcccaga gacacagtag agagcgcttc agcccgccgg agaaggtagg caagaaataa
     3061 gacggtggcg ccaaaataga gcgccatcgt caccataaaa aacacggcgg ccatcgaggt
     3121 ctatccccgc aacttgtaag gacaaactga gcggtcttta
//
LOCUS       DATDNW010000024         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_275467, whole genome shotgun sequence.
ACCESSION   DATDNW010000024 DATDNW010000000
VERSION     DATDNW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275467"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VI359_00615"
     CDS             <1..840
                     /locus_tag="VI359_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972781.1"
                     /translation="FLFLVLATLFLTPLPCLAIPAEGEPPPDPHLNDPKATIILVRVV
                     AYGSMVLGKDVGGARVTITDIATKEILATGLQQGEAGDQNQIMRTPHVMGEPRFSSGP
                     SAVFTATLELTRPTLVEVTAEGPLAYPMAAQRVSKTVWLIPGHDLTNDGIVLHLYGYI
                     IQIEHPKPGESLIAKDDVILRASVRTLSGALVRPHGDWDSRKAHIYGEVLIGDRVIER
                     LQMFYSGERASFEAPFFVPVPTDAPDGITLRVVAADPASGNFGVGQAKFPVLSERLPP
                     RKP"
     gene            849..1547
                     /locus_tag="VI359_00620"
     CDS             849..1547
                     /locus_tag="VI359_00620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6972782.1"
                     /translation="MRKTRLRPSIPLFIVLAWWSLSTGPAVAQDQHQHRRPDNIAQYL
                     EHLDSSERDQYQKPIQVIEALNLKPGQAVADLGSGSGYFTRRLVGSVTDAGKVYAIDV
                     EPEMLRYVEDSLVHTHAPYNAEFILARSDSPKLPVESVDLIFVCNVFHHLEDRTTYFR
                     NARTALKPGGRIAIIDFYHDERSGDLGFSKQHLVPRDTVIAEMTTAGYRLEREHSFLP
                     RQYFLEFVPQPPVP"
     gene            1616..2560
                     /locus_tag="VI359_00625"
     CDS             1616..2560
                     /locus_tag="VI359_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY6972783.1"
                     /translation="MTTLASYQELRFRLSLALAVNAVIITAEFIGGFVLDSIGLMSDA
                     GHNLIDQGSLFLALYAHLLTTKPASETRTFGYHRAGIIAAFLNSFILLLTAVGIALVG
                     IKRLLHPVPVDGVWVMAIAALSFVANLSIALLLQHGAKDDLNIRSAFWHMLGDAWVSL
                     GVVISGGTILLTGWTILDPLVSLLVVGAIVKGAWPLFKESLEVLLESTPPRISASHVA
                     TMIESVPGVKNVHDLHIWAVEPRLIMLTCHVLVDGEDDALTNQLLRTIRGRIASDFGI
                     KHMTIQMETACCHPEAVHCDLSKLADQHHEFEIASHHH"
BASE COUNT          565 a          826 c          702 g          603 t
ORIGIN      
        1 tttctttttc tcgttctggc cactctgttt cttacccctc ttccctgtct tgcgattcct
       61 gcggaaggcg agccgcctcc cgacccccac ctcaacgatc cgaaagcgac gattatcctg
      121 gtgcgtgtcg ttgcctatgg ctccatggtt ctgggcaagg acgtgggcgg agcgcgcgtt
      181 acgatcactg atatcgccac aaaagagatt ctcgcaaccg gtctccaaca gggcgaggcc
      241 ggcgaccaaa atcaaatcat gcggacgccc catgtgatgg gtgaacctcg cttcagttcc
      301 ggaccctccg ccgtgtttac ggccacactc gaactcactc gcccgaccct ggttgaagtg
      361 accgccgaag gaccgctggc ctatccgatg gcagcacaac gggtctcgaa aaccgtctgg
      421 ctgattcccg gacatgacct gacgaacgac ggaatcgtcc tgcatctgta tggctacatc
      481 attcaaattg aacaccccaa accaggtgaa tccttgatcg ccaaggacga cgtcattctc
      541 cgggcctctg tgcgcacgct ctccggcgcg ctcgtgcgac cgcatggaga ttgggattca
      601 cgcaaggccc atatctacgg agaagtgttg atcggggatc gcgtgatcga acgccttcag
      661 atgttttaca gcggcgaacg agccagcttt gaagcaccat tttttgtgcc ggttccgaca
      721 gatgcccctg atggcatcac cctgcgagtg gtcgcggctg atccagctag cgggaatttc
      781 ggcgtgggac aggctaagtt tcctgtcctc agcgaacggt tgcctccacg aaagccatga
      841 cgctcgccat gagaaaaacc cgcctacgcc cttcgatccc cttattcatt gtccttgctt
      901 ggtggtcgtt gtccaccgga ccagccgtcg cccaagacca gcatcagcac cggcgaccgg
      961 acaacatcgc gcaatacttg gaacacctcg acagttccga gcgggatcag taccagaaac
     1021 cgatccaggt catagaggcg ctgaatctca agccgggtca agccgtggcc gatctcggat
     1081 caggctcggg ctacttcacc aggcgattgg tcggatccgt aacggacgcc ggaaaggtct
     1141 atgcgatcga tgtggaaccg gaaatgttgc ggtacgtcga ggatagcctc gtgcacacgc
     1201 atgctcccta caacgccgaa ttcatcctcg cacggtcgga cagccccaag ctgcctgtcg
     1261 aatcggtcga tctcatcttt gtctgtaacg tctttcatca tctggaagat cgcacaacct
     1321 acttccgtaa cgcgagaaca gctctcaagc ctggtggacg aatcgccatt atcgacttct
     1381 atcacgacga gcgatccggc gatcttggat tttcgaaaca gcatcttgtc cctcgagata
     1441 cagtcatcgc tgagatgacg acggccggat accggctcga acgtgaacac agttttctcc
     1501 cgcgccagta ctttctcgaa tttgttccac aaccccccgt tccataactc gagccgcacc
     1561 ttagcaactc gctcgaacgc ttgctgtctt ccatcggcca tgcgacacta tgaccatgac
     1621 gacccttgca agctatcagg aactgcgatt tcgactctct ctcgccctcg cagtgaatgc
     1681 cgtcatcatc acggccgagt tcatcggtgg attcgttctc gacagcatcg gactgatgag
     1741 cgatgccggg cacaacctga tcgaccaggg gtcattgttc ctagcccttt acgcacacct
     1801 gctgacgacg aaaccggcca gtgaaacacg cacctttggg tatcatcgag ccggcatcat
     1861 cgctgccttt ctgaactcgt tcattctcct attgaccgcg gtcggaatcg cgctggttgg
     1921 catcaaacgg ctgcttcacc cggttcctgt cgacggcgtc tgggtgatgg cgatcgcggc
     1981 attgagtttt gtggccaatc tcagcatcgc gctcctgctg cagcacggcg ccaaagacga
     2041 tctcaatatc cgcagcgcgt tctggcacat gctgggggac gcgtgggtct cgctcggtgt
     2101 ggtcatcagc ggcggaacca ttttgctgac cgggtggacg atcctcgatc cccttgtgag
     2161 tctgctcgtc gtcggagcga tcgtgaaagg agcgtggccg ctgtttaagg aatcactgga
     2221 ggtactgttg gaatcaacgc caccgcggat cagtgcctcc cacgtcgcca cgatgattga
     2281 gtctgttcct ggtgtgaaaa acgtccatga tcttcatatc tgggccgtgg aaccgcgtct
     2341 aatcatgctc acgtgccatg tgctcgtcga tggagaggat gacgcgctca ccaatcagct
     2401 tctccgaacg atcagaggtc gcatcgcgtc agacttcggc atcaagcata tgaccatcca
     2461 aatggaaacg gcctgctgcc acccggaagc ggtccactgc gacttgtcga agctggccga
     2521 tcagcaccac gagtttgaaa tagcctctca tcatcattga gctttccccc acacgcagct
     2581 ctcggtagaa taccgttcga tacgcaatcc gtttgacgaa agacccttca tcgaggttca
     2641 actatgccgg tcccgtttta tgtactctgt ggctcgttag gagcaggaaa gaccac
//
LOCUS       DATDNW010000025         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_822909, whole genome shotgun sequence.
ACCESSION   DATDNW010000025 DATDNW010000000
VERSION     DATDNW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822909"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VI359_00630"
     CDS             <1..563
                     /locus_tag="VI359_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972784.1"
                     /translation="AYSLFMHHSSGIALLAVGLLMLLDRLWLCPRDFTGVLIGCTWLL
                     FGMFVFIMGDPEGWPIGFAGFIESFSMPTASEWIQHKLLSLIPMVLGIYSITIRRIPP
                     SINLAGWVAGLALLGGIGLIVHQHANHPDFDIVNLQHWIFALTSFFIAASLLVERSEA
                     ITAKSKSFFVPLEVMVLGLQLALYVE"
     gene            576..1796
                     /locus_tag="VI359_00635"
     CDS             576..1796
                     /locus_tag="VI359_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6972785.1"
                     /translation="MVDGFSFLEWACLVPVVAGSLYSLVCLVCVLIVRAQASIPPRVP
                     IGGWPPVTILKPVHGLEKNLEKNLRSACLQDYPTYQVVFSVQNENDPAIPLLRDLEGE
                     FGCERATVVIEQRQVGTNGKINNLVGGLAHAQHEILVISDSDVYLGPNYLKAIVSPLD
                     NPDVGFVCTFFKATSAGSWFEKMELLTMNASFFPDTAFAHVTKTAKFCIGSSVALRRS
                     TLNNIGGLESLADYLVEDYEMGRRIWEQGKKAAVAPYVVETVVDLKSPSQWWNHQVYW
                     DQNSCIVRPGALISTLIIRPIPFAFLFALLRLADPLGLLVLAGAVGWRLAVAVGILGY
                     GLGDREGLKSLLLLPLRDVAGLVSLILAFTQKTTVWRGKEYVLTRDGRMVPREVSVCK
                     NSSSPETTSALPSR"
     gene            1745..2596
                     /gene="hpnK"
                     /locus_tag="VI359_00640"
     CDS             1745..2596
                     /gene="hpnK"
                     /locus_tag="VI359_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEY6972786.1"
                     /translation="MQKLIITGDDFGLAVPVNEAIEEAHRNGVLTSASLMVAAGAAKD
                     AVARAKRLGSLRVGLHLVLVDGQAVLPKWSIPDLVDEQGEFLPDPLLVGLRYFFESDL
                     RPQLEAEIRAQFQAFRDTGLPLDHVNGHNHLHLHPTVLGLILKIGREFGLRAMRLPYE
                     PPLPSWRASGRGLLRKSTGWMLLFPWIVFLKARLKLAGIRCNDFVFGLNDTGHLHREL
                     LLRILRELPDGVTEIYCHPATRRCAELDRTMPGYMNEQDFEALTSQAVKQAVAASGFQ
                     RVAFDEL"
     gene            2637..3620
                     /locus_tag="VI359_00645"
     CDS             2637..3620
                     /locus_tag="VI359_00645"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6972787.1"
                     /translation="MRTYLVTGGAGFIGSHVAEVLVKRGESVKVLDNFFTGKRENLAS
                     IDGAFEFIEGDVRDAALCQEILEGVDAVVHLAALHEVVRSVECPMETHDVNVTGTLNL
                     LLGAHQAGVKKFVFASSSAVYGENPAIPRVETMTPAPSSSPYAVTKQSGEYYCQLFST
                     LYGLETVCLRFFNVYGPRQDAASTYAAVIPKFISLLLAETAPIIYGDGEQSRDFTFIG
                     DCVAGVLTACELPGLSGQVLNIGTGRCTTINQLYAQLQILLGTNLSPHYGPRQPGDLP
                     HDQADIGQAKRFLHFQPRYGLARGLQETIEFMRYAHPSEKSSITSTRELRV"
     gene            3635..>3934
                     /locus_tag="VI359_00650"
     CDS             3635..>3934
                     /locus_tag="VI359_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEY6972788.1"
                     /translation="MKTLLLNPPSFDMFDGGASSRYQATREIPSFWYPVWLAYSAGMI
                     KDSRLLDAPSHGVSPEETVALAKDYEFLALFTSTPGFKSDVRLAQMIKEVNPRIKI"
BASE COUNT          820 a         1071 c         1081 g          962 t
ORIGIN      
        1 ttgcctattc tctgttcatg catcacagct ctggtattgc tctgttggcc gtagggctgc
       61 tcatgctgct cgatcggctg tggctctgtc ctcgtgactt tacaggggta ctgatcggat
      121 gcacgtggct gctgttcggc atgtttgtgt tcatcatggg cgacccggag ggctggccga
      181 tcggatttgc tggattcatt gagagtttca gcatgccgac ggcgagtgaa tggatccagc
      241 acaagctcct ttcgttgatc cccatggtgc tagggatcta ctcgatcact atcaggcgca
      301 ttccgccgag cattaacttg gcaggttggg tcgccggtct cgcgttgttg ggtggcatcg
      361 gactgatagt gcaccagcat gcgaatcatc cggatttcga cattgtgaac ctccaacact
      421 ggatctttgc tcttacctcc ttttttatag ccgcgagttt gctagtggaa cgatccgaag
      481 ccatcacggc gaagtccaaa tcgtttttcg tccctctaga agtcatggtc cttggtctcc
      541 aactcgcact gtatgtggaa tagggtcacg aggtaatggt ggacggattt tcctttctcg
      601 aatgggcgtg tttggtccca gtcgttgctg gctcgctcta ttctcttgtg tgcctagtct
      661 gtgttttgat tgttcgagca caggcctcaa tcccccctcg agtcccgatc ggtggctggc
      721 ctcctgtcac gattctgaaa ccagtgcacg ggctcgagaa gaatctggag aagaatctcc
      781 gtagcgcctg tctgcaagac tacccaacat accaggtcgt cttttctgtg cagaatgaga
      841 atgatcccgc gattcccctg ttgagagatc ttgaggggga attcggctgc gagcgggcca
      901 ccgtcgtcat tgaacaacgg caagtaggaa ccaatggcaa gatcaacaat ctggtcggcg
      961 ggttagccca tgcacagcat gagattttgg tgatcagcga cagcgatgtg tacctggggc
     1021 cgaattatct taaggcgatc gtctcccccc tggacaatcc agacgtcggc ttcgtctgca
     1081 cgttttttaa ggcgacgtcg gcagggtcct ggttcgaaaa aatggaactg cttacgatga
     1141 atgcctcctt cttccctgac acagccttcg cgcacgtcac caaaacggcg aaattctgta
     1201 tcgggtcgtc agtcgccctt cgacggtcca ccctgaataa tatcggtgga ttggaaagcc
     1261 tcgccgatta tttggtcgaa gattacgaaa tggggcgccg catatgggaa caaggcaaga
     1321 aagctgcggt ggcgccctat gtggtcgaaa ctgtggtcga tctgaagtct ccttcccagt
     1381 ggtggaatca tcaggtctac tgggatcaaa acagttgcat cgtgagaccc ggcgccctca
     1441 tctcgacact catcattcgc ccgatcccct ttgcgtttct ctttgccctc ctgaggctag
     1501 ccgatccgct cggattgttg gtgcttgcag gagccgtcgg gtggcgcctg gcggtggctg
     1561 tcgggatact cggatatggc ctcggagacc gggagggtct caagagcctc ctgcttcttc
     1621 ctctcaggga cgttgcaggg ttggtgtcac taatcttggc gttcacgcaa aagaccacag
     1681 tatggcgggg aaaagaatac gtcctcacca gagatggacg catggttcct cgggaggtgt
     1741 ctgtatgcaa aaactcatca tcaccggaga cgacttcggc cttgccgtcc cggtgaatga
     1801 ggccatcgag gaagcccatc gcaacggcgt cctcacatcg gccagtctca tggtcgcagc
     1861 cggagccgcg aaggacgcgg tcgcgcgagc caaacggctc ggttcattaa gagtggggtt
     1921 acatctggtg ttggtagacg gtcaagccgt gttaccgaaa tggagtatcc ccgaccttgt
     1981 ggacgagcaa ggtgagttcc tcccggaccc gcttctcgtc ggattgagat atttcttcga
     2041 gtcagactta cgtccacagt tggaagcaga gatccgcgcg cagtttcagg ccttccgtga
     2101 cacaggactt cctctcgacc atgtaaacgg acacaaccat ctgcatcttc atcccactgt
     2161 ccttggactg atcctcaaga tcggacggga atttggctta cgcgcgatgc gacttcccta
     2221 tgaaccaccc ctcccatcct ggcgcgcgtc cggacgaggg ctcttgcgta aatccacggg
     2281 ctggatgctc ctcttcccct ggatcgtctt cctgaaagcg aggctaaaac ttgccggaat
     2341 ccgctgtaac gattttgtct tcggtctgaa cgatacaggt cacctccatc gcgagctctt
     2401 gcttcgtatt ctgcgagaac ttccagatgg tgttactgaa atttattgcc atcctgccac
     2461 ccggcggtgt gcggagctcg accgtaccat gcctggctat atgaacgagc aggactttga
     2521 ggcactgacg agtcaggcgg taaagcaggc agtcgcagcc tctggatttc agcgagttgc
     2581 atttgacgag ctgtaaacac gagcgcgctt cttttgatca tagggtggga gggacgatgc
     2641 gaacatatct ggtcaccggt ggagctgggt ttatcggctc gcatgtagct gaggtgcttg
     2701 tgaaaagggg cgagagcgtc aaagtcttgg acaatttctt taccgggaaa cgggaaaatc
     2761 ttgcgtcgat tgatggcgct ttcgagttta tcgaaggcga tgtcagagat gctgcacttt
     2821 gccaggaaat tctagaggga gtcgatgcgg tcgtgcatct ggctgcattg catgaagtcg
     2881 tgcgctccgt tgaatgtccc atggaaaccc acgacgtgaa cgttaccggc acattgaatc
     2941 tgctcttggg cgcgcaccaa gcaggcgtga aaaagtttgt attcgcgtca tcgtccgcag
     3001 tctacgggga gaatcctgca atccctcgag tagaaaccat gacccccgct cccagctcat
     3061 ctccctatgc cgtgacgaaa caatccggcg agtactactg ccaactgttc tcaaccctct
     3121 atgggttgga gacggtctgt ttgcggtttt tcaacgtcta tggtcctcga caggatgccg
     3181 cctcaaccta tgcggccgtg atcccaaaat ttatctccct cctcctggca gagacagccc
     3241 cgatcatcta tggagatggg gaacagtcac gcgatttcac attcattggc gattgcgtgg
     3301 ccggtgtgct gactgcctgt gagcttcccg gcctctcggg gcaagtgctc aacatcggga
     3361 cgggccggtg tacgacaatc aatcagctct atgctcaact tcagatcttg ctgggcacaa
     3421 acctgtcccc ccattacggg ccgcgtcagc ctggggatct gccacacgat caagcggata
     3481 ttggacaggc gaaacgtttt ctccatttcc agcctcgata cggcttagct cgcggcctcc
     3541 aggaaaccat cgagttcatg cgatacgctc accccagtga aaaatcttcc atcacaagca
     3601 cacgggagct tcgtgtctga cagaggagat accgatgaag acgttactgc tcaacccccc
     3661 ttcatttgac atgtttgatg gaggagctag ttcgcgatat caagctacca gagaaattcc
     3721 ttcgttctgg tatccggtgt ggctcgccta ttctgctggc atgattaaag acagccggct
     3781 gcttgatgcg ccctctcatg gcgtaagccc cgaagagacg gtcgccctcg cgaaggacta
     3841 cgagttcctc gcgctcttca ccagcacgcc aggatttaag agcgacgtac ggctggctca
     3901 aatgatcaaa gaggtgaacc ctaggatcaa gatc
//
LOCUS       DATDNW010000026         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_822979, whole genome shotgun sequence.
ACCESSION   DATDNW010000026 DATDNW010000000
VERSION     DATDNW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822979"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            816..2399
                     /locus_tag="VI359_00655"
     CDS             816..2399
                     /locus_tag="VI359_00655"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972789.1"
                     /translation="MTPVRTMITILMALSLVLWNTSGQAQGFTESWYMSRGNSNMEIG
                     NYKAAIEAFEKVVEINSNNREAMRSLGIAYERQGLKDKAIEQFDRYLAKVDDDPEIAF
                     KQAQALEWSRYAYREKDMLKYYKMGLKRKDDPRMRLKYATHLARYKESSQEAIVQFEK
                     VLVREPRNVEAHRGLAKAYAWLGDNDRALYYTNLARDYARREPGDMTVLRQEMMKGRE
                     PTAEGTLGFLVQPERPFELYGFRLGSRGKMDLTAFTTTTLEAGAEHFWNSSENRSGGY
                     ISLGNQVRFNPTNRIDGIIEYHGVTRADGLAYKFEYTHEGESFSIRPGVKREFRYDSF
                     ASLVGSRTTGQLLGVARSTLFYTTVTFEARDIRFNVTPLIGWVSTETLSNNTQVGLDT
                     KTAIPVWSDENWEVAAEYVFYLTHYDQDQSGFSPSAVEPLPGGYFSPRVFVNQIPRLA
                     ATYVGENKNEFYVAAGPAVQYIDEATKSAAFRIGGDFHASYTAPLSQRFLFKVMTDYT
                     QIATVYIRFQTNALLVYTF"
     gene            complement(2626..2701)
                     /locus_tag="VI359_00660"
     tRNA            complement(2626..2701)
                     /locus_tag="VI359_00660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2666..2668),aa:Lys,seq:ttt)
     gene            complement(2767..>3142)
                     /locus_tag="VI359_00665"
     CDS             complement(2767..>3142)
                     /locus_tag="VI359_00665"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY6972790.1"
                     /translation="GVVARYATMVNGMTSLAVTKLDVLDGCKELKLCTGYRYNGRVFR
                     DMPADLQALTHCEPVYRTFKGWTAPTTGVRTYKGLPSEAKRYLQAIEDLGECRIDMIS
                     TGSKRDETIVLRNPLKARSRAR"
BASE COUNT          719 a          864 c          870 g          689 t
ORIGIN      
        1 ggacgcgtat tttttcaatt tgagttgggc gttgtttaat ctgctgttct tgctggcagg
       61 ccttttgatc gcctgggaga aaccgcaacg gcgtctggag gaccggatta gaaagtctgt
      121 tccatttacc ctccagggag acgggttctt cttgcaggga cacacggctg acctgagtgc
      181 aagcggagtg tcattcatca ccacgacgag cgaggcaatt ccgccgtccg tcgaaatcag
      241 tctgcaggac cgacaccctg tcatctgtca atgccagttg gtctaccatg agcgtatatc
      301 ggctcgtgcc taccgatgcg gacttgtatt cctcgacacg agacacgaaa tccgccgagc
      361 attgattttg aatctgtttg ctgatccagc aacctggcgg gaggcgcatg tacaccgaac
      421 gcgcagtaat ttggtcatgg cttttcattt cttgcgaggg attgtgcgct gtctccgacc
      481 gatgagaaca acaatccgcc gttgtatacg tcgtcagaca ctgcagctct tgcgggttca
      541 aactgattcg caccgacagc aggtgatcgt gagagattgg tccccgcgtg gcctgggcct
      601 tctgtggata ggccgcacgg ttccatcgac cttgcaatgg accgtgctca ctccgcaatt
      661 tggttgcgcc cggtttcgcc tgatctatcg gcgacaactc ataccatgtc tttggcgcgt
      721 agggttagaa gccgagccta cgctcaacgc cttgcgcacg ccagaactcg cactgtcagc
      781 gcagatcgga cactcgcccc aatgaggtgc tatcgatgac acccgtccga acgatgatca
      841 ccatcctgat ggcgctgagt ctggtcctct ggaacacgag tgggcaggcc caagggttca
      901 cggaatcctg gtacatgtct cgcggcaatt ccaatatgga aatcggcaat tataaggccg
      961 ctattgaagc gtttgaaaaa gtggtggaaa tcaactcgaa taaccgagag gccatgcgca
     1021 gtctggggat cgcctacgaa cggcagggcc taaaggataa ggccatcgag cagttcgacc
     1081 gctatctcgc caaggttgat gatgatcccg agatcgcctt caaacaggcg caagccttgg
     1141 aatggtcgcg gtatgcctat cgcgaaaaag acatgttgaa atattacaag atggggctca
     1201 aacgaaaaga cgaccccagg atgcgtctca agtacgccac gcatttggct cgatataaag
     1261 aaagcagcca agaagccatc gtccaatttg aaaaggtgct agtgagagag ccccgcaatg
     1321 tcgaggccca tcggggccta gccaaagcgt acgcctggct cggtgacaac gaccgagcgc
     1381 tctattacac caatctggcc cgcgactatg cgaggcggga gccaggcgac atgacggttc
     1441 tgcggcagga gatgatgaaa gggcgggaac cgaccgcaga gggcacgttg ggattcttgg
     1501 ttcagcccga acggcccttc gagctgtatg gatttcgtct gggctcgcgc ggcaaaatgg
     1561 acctgacggc attcaccacg accacgctcg aagccggcgc cgagcacttc tggaactcgt
     1621 cagaaaaccg ctccggcggg tatatctcgc tcggaaatca agtccggttt aatccgacaa
     1681 accggatcga cggcatcatc gagtaccatg gagtaaccag agctgatggg ttagcctaca
     1741 aatttgagta cacgcatgag ggggagtcgt tctccattcg accgggggtg aagcgagaat
     1801 tcaggtatga ctcgtttgct tcgctcgtgg gatccagaac gaccggacaa ctcctgggag
     1861 tcgcgcggtc caccttgttc tataccaccg tgacgtttga ggcccgcgac atccgcttta
     1921 acgtaactcc gttgatcggc tgggtgtcga ccgagaccct cagcaacaat actcaagtgg
     1981 gattagacac caaaaccgcc atacctgtct ggagcgatga gaattgggag gttgccgcgg
     2041 agtatgtgtt ttacctcact cactatgacc aggatcaatc cgggttcagt ccgagcgccg
     2101 tggaaccgtt accaggtggc tactttagtc cccgagtctt cgtgaatcag attcctcgac
     2161 tggcagccac gtacgtcgga gaaaacaaga atgaattcta cgtggcggcg ggaccagccg
     2221 tgcaatacat tgacgaagcc acgaaatccg ccgcctttcg tatcggcggc gatttccacg
     2281 cctcctacac agctcctctg agccagcgct ttctcttcaa ggtaatgaca gactatacgc
     2341 agatcgccac cgtgtatatt cgctttcaga cgaacgcgtt actcgtatac acattctagc
     2401 ctaggggcaa ttcaccttca gcagcgcata ttccggctgt tgtgcctcac ttaacagttg
     2461 gacagatttc cccgctgtct ccattaattc ttcctgcttc ttatacgacc tgaccacaac
     2521 ttccgtggga cacaacaaga gaggttttga acacggtccg cagaacgact agatgggcga
     2581 aaagacgctg aaaccggttt tgaaggttct atctgtggtc caaagtggtg agccgcctgg
     2641 gactcgaacc cagggccctc gccttaaaag ggcgatgctc taccgtctga gctagcggct
     2701 caccgaatgg agagggggga ttctaccatc gataatcaat gtgtgcctag agaggacgtt
     2761 gataacctag cgggctcgcg agcgagcctt gaggggattc cgcaagacaa tggtctcgtc
     2821 acgcttcgaa ccggtcgaga tcatgtcgat ccgacattcg cccagatctt cgatggcctg
     2881 taggtaccgt ttggcttccg acggcagtcc tttataagtt cgaacgccgg ttgtgggagc
     2941 ggtccagccc ttgaacgtgc gatacaccgg ctcacagtgg gtcagcgcct gcagatcggc
     3001 tggcatgtct cgaaagaccc ggccgttgta gcgatatccc gtgcagagct tcaattcctt
     3061 gcacccgtcc aagacatcga gcttcgtgac ggcgagcgag gtcatgccgt tcaccatggt
     3121 ggcgtagcgt gcgaccactc ca
//
LOCUS       DATDNW010000027         7980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1300194, whole genome shotgun sequence.
ACCESSION   DATDNW010000027 DATDNW010000000
VERSION     DATDNW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300194"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VI359_00670"
     CDS             complement(<1..621)
                     /locus_tag="VI359_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY6972791.1"
                     /translation="MAVTIHNTRLINGAGAVIERATVVVRGATIAAVGPSKDVAVPRG
                     ATRIDGRGFTLLPGLIDCHVHLCLGGEPDVVGTLARETPSLTLLKSSRAAQQTLEAGF
                     TTVRDVGARDHSIFTLKQAIEAGLVPGPRIVGAGLAICMIGGHARFIGQEVQGTDQVR
                     SVVRAQIAAGAEVIKVIASGGVLTPGTSPDQAQMTPDELQAAVEEAQ"
     gene            complement(626..1219)
                     /locus_tag="VI359_00675"
     CDS             complement(626..1219)
                     /locus_tag="VI359_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6972792.1"
                     /translation="MNRPLTVVLGAATFAFIAVVVFWIFKLTLADSMKNDMVSSEKVA
                     AFIHAVLEANRNNYTENVVSKLRRDGIEAHEHWRDERGVPLPAQFLMETGRLVALKDL
                     NFSFRLASLTPVYVWNGPNSDLERQGLAAVQQNPDHPYYGFVTKSGTRYFQAIYADRA
                     VSIACVECHNNHPNSPRRDFKLNDVMGGIVITFPVGE"
     gene            complement(1239..2417)
                     /locus_tag="VI359_00680"
     CDS             complement(1239..2417)
                     /locus_tag="VI359_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY6972793.1"
                     /translation="MHEFLPPQRLLLGPGPSQVHPRVLRALATPLLGHLDPAFLTVMN
                     DIQTLLRNVFQTTNRFTIAVSGTGSAGMEAAVVNLVEPGDSVIVGVNGAFGARLATVV
                     ERCGGKAIRVETPWGDIIEPDMVEQALRRSGPVKAVAVVHAETSTGAWQPIEPISRLC
                     RDHGALFIVDAVTSLGGVPVEVDQWGIDACYSGTQKCLSCPPGLAPLTLSDRALAAIK
                     ARRTPCQSWYLDMALVADYWADSTRTYHHTAPVSMLYALREALRLVEEEGLPARFRRH
                     RLNSEALVAGLIELGLIPLPPAEHRLPMLTCVKLPGQIDDAAVRTKLLQTFGIEIGGG
                     LGPLKGKVWRIGLMGESCTEANVLTLLNALEEILFRSGGLSTPGVALQAAARAYSRTQ
                     "
     gene            2563..4065
                     /locus_tag="VI359_00685"
     CDS             2563..4065
                     /locus_tag="VI359_00685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY6972794.1"
                     /translation="MESETTASPPSLSTSSWWFEVNPPPAVGDYSNRFSPIVSVLTAL
                     FLVAALSSVVWLSSSIPKLERVEAPERALALMVSRIMDVQEGMKRAPTWQQTLFGWIS
                     GDNELERAQAIAWFQELAEMSDNALVPLQLAVLQAESGHVSQALLLAHEWSQAVEPLP
                     QLASLVVAAYGEGPLPSGETFEALQAELAELVPTGWFYNRLAERLAQRANNQPLLDTI
                     REAYAPRVDRQFARARAITALEFTAMIVGTVVLVIFWITRARVPNVVRLHEPGVPPPW
                     PGGIGAAVLLRGGAIGAMLTVVFLLYMPQENASLRALAIPLTNLPLLLLAYQYLFKPA
                     QMTFDQGFGLEIGWARAGRLVAAVLAVMAAGLWGEWIMDRMAEPLHLTNHWTEWFDAD
                     LVWGPSSLTAISLVEYVAFAPVFEELAFRGLLFAILRRKFRFLPAALMSASVFAIAHG
                     YGLVGFVSVLWSGLLWAWIYEKTGSLLPGILAHAINNLLVCLSVMALLRW"
     gene            complement(4108..4953)
                     /locus_tag="VI359_00690"
     CDS             complement(4108..4953)
                     /locus_tag="VI359_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972795.1"
                     /translation="MKKWETERATASVLRQKRLPLTVLGSGYVGRFLIAEACCQSRIV
                     LSTSRDPDRRLTHVPPPQRLRFDLAQPDTWTNIPTNTDIVWCFPATPLEQVRAFAKGL
                     GVPPRRLVVLGSTSAYDRPSQSTDYPPPWIDESAPVDLRKPRVRGEEYLRKEHGAIVL
                     RVAGIYGPGRNPIEWIRSGRVRPTRKYVNLIHVEDLAAICMLALETGTPGEVYNVSDG
                     TPHTWADICRVAQTRWDVAIHAGANDNRPGKRIKTIALREKLGATLRHPDLYAELEQL
                     EGTRR"
     gene            5013..6662
                     /locus_tag="VI359_00695"
     CDS             5013..6662
                     /locus_tag="VI359_00695"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY6972796.1"
                     /translation="MKSIASLMTIAGLAVTLSGCPDSGGGGGNASNLAITTTSANFAV
                     AGNPYSSTLAATGGTPPYTWAAVSVLPGWVLLNPMTGVLTGTPGTSDVGNFNLTFRVK
                     DTTGATAVGTVLLAVHNRTDILSVDNSTPPVPGGAASSSPSISNDGRFVAFVSTANLI
                     PGVGGSQIYLHDWQTNQTSLISRDSGVAINAGNGISSAPSISSDGHFVAFVSNSTNLV
                     SGVSGQQIYLRNVQTGVTTLVSKDNLNNPATLGSTTTSPSISSTGRFIAYVSNATNLV
                     AGVLGGQQIYLYDTQITMAFPNGQTTLVSKDNSPTPLPGDGASSAPSISQDGCFVAFA
                     SLSTNLISPSPGNQQVYVRGPLPGPGTCGAPEQTSLMSQDNSASPLPGNGFSSSPSIN
                     SDGRFVTFTSASSNLLTGVSGQQIYLHDRNSGPNGTTSLVSKDNSALPVQGNGSSSVP
                     SISQDGCFVAFASLSTNFGVAGGNQQIYIRGPLPGPGTCGVPELTSLVSKDNSVVPVA
                     GNAASDAPSVNANGGFVAFSSLSTNFGVAGGNQQIFVRAMP"
     gene            6707..>7980
                     /locus_tag="VI359_00700"
     CDS             6707..>7980
                     /locus_tag="VI359_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6972797.1"
                     /translation="MPLTLFHPLIAEWFSSSVGVPTDVQQAAWPAIQSGQDALIAAPT
                     GSGKTFAAFLSCIDHLFKQALARELDDHTQVLYVSPLKALSNDVQKNLQQPLNEIGQA
                     ALQAGFLMPELRVLVRTGDTPMVDRQQMLKRPPHILVTTPESLFILLTAEKSRRMLQT
                     VRTVIVDEIHAVAPNKRGAHLALSLERLEALTLNKPQRIGLSATQRPIETVAQFLVGD
                     RPMPTIIDVGHKRAMDVAVEVPKDELSAVATNAIWTDVYDRLAELIRQHRSTLVFVNT
                     RRLAERVSHHLEERLQDLGADAVAAHHGSLSRQIRLSAEERLKLGKTRVVVATASLEL
                     GIDIGTVDLVCQLGSPRAIATCLQRIGRAGHWIKAIPKGRLFALTRDELLECAALVRA
                     IRRGVLDHISVPPAPLDVLAQQVVAAAASQTW"
BASE COUNT         1600 a         2340 c         2341 g         1699 t
ORIGIN      
        1 ctgagcctcc tccacagcag cctggagctc gtccggggtc atctgtgcct ggtctggtga
       61 cgtgcctggc gtcaacacac cgcctgaggc gatcactttg atgacctcag cccccgcggc
      121 aatctgcgca cgtaccacgg aacggacttg atcggtaccc tgtacctcct gcccgataaa
      181 ccgggcatgg ccgccgatca tgcaaatcgc gagaccggct ccaacaatgc gggggcctgg
      241 cacaagcccg gcctcaatgg cttgcttcaa ggtaaaaatg gaatgatcgc gcgctccgac
      301 gtcacgcaca gtggtgaagc ccgcttccaa agtctgttgc gccgcgcggc ttgatttgag
      361 caaggtgagg gaaggcgttt ctcgtgcaag cgtcccgacc acgtccggtt cgcctccgag
      421 acaaagatga acgtggcaat cgatgagtcc cggcaacagg gtgaagccgc gtccgtcgat
      481 ccgtgtggcc ccacgcggta cggccacgtc cttgctcgga ccgacggccg caatcgtggc
      541 gcctcgaacg accacggtgg cacgctcaat caccgcaccg gccccgttga tcaggcgcgt
      601 gttgtggatc gtgacggcca cgagactact cgccgacagg gaaggtgatt acgattccgc
      661 ccatgacgtc gttcagtttg aaatcgcgac gaggactgtt gggatggttg ttgtggcatt
      721 caacgcacgc gatcgacaca gcccggtcgg catagatcgc ttgaaaatac cgcgtgccgc
      781 tcttcgtgac gaatccgtag taggggtggt ccggattttg ttgaaccgcg gcaaggccct
      841 gtcgctcgag atcgctgttg gggccgttcc agacatagac cggggtcaga ctggcgagcc
      901 ggaaagagaa gttcaaatcc ttgagcgcga caagacgccc tgtttccatc aggaactgcg
      961 ccggcagcgg cacgccccgt tcgtccctcc aatgttcatg ggcttcgatc ccgtcacgcc
     1021 gaagtttgct gacaacgttc tcggtatagt tgttccggtt ggcttcaagc acggcgtgga
     1081 tgaaggccgc caccttctcc gatgaaacca tatcgttctt catcgaatcg gcgagcgtca
     1141 gcttgaaaat ccaaaacaca acgacggcaa tgaatgcgaa tgttgccgcg cctaggacca
     1201 ccgtcaaagg acgattcatg attctgtctt tctccggact actgtgtccg gctataggct
     1261 cgcgccgcgg cttgcaatgc cacacctggc gtcgagaggc caccggaccg gaataaaatt
     1321 tcttcgagtg cgttcagcaa ggtcaacaca ttggcctcgg tgcaagattc tcccatgagg
     1381 ccgatgcgcc agacctttcc cttcaacggg cccaagccgc caccaatttc gatcccgaag
     1441 gtctgcagga gcttcgttcg cacggccgca tcgtcaatct gaccggggag cttgacgcag
     1501 gtcagcatcg gtaaccgatg ttccgcaggc ggcagcggaa tgaggcccaa ttctatcaga
     1561 ccggcgacca gcgcctcgct attgagccgg tggcgtctga aacgcgcggg caatccttct
     1621 tcttcgacca gccgtaacgc ttcccgcagg gcatagagca tcgaaacagg agcagtgtga
     1681 tggtaggtgc gtgtgctgtc ggcccaatag tccgccacca gcgccatgtc caagtaccaa
     1741 ctctgacacg gtgtgcggcg cgctttgatg gcggcgaggg ctcggtcact caatgtcaaa
     1801 ggagccagtc ccggaggaca gctgagacat ttctgagttc cgctgtagca agcgtcgatg
     1861 ccccattgat cgacttccac cggcacgccg cctagcgagg tcacggcatc gacaatgaag
     1921 agggcgccat ggtcgcgaca taggcgagag atcggttcga tgggctgcca cgccccggtt
     1981 gaggtttcgg catggacgac agcgacagct ttcacaggtc ctgagcggcg caaggcctgt
     2041 tcaaccatgt ctggttcgat aatgtctccc cagggggttt ccacgcgaat ggctttcccg
     2101 ccacaccgtt caacgacggt cgcgagtctg gcgccgaaag ccccgtttac cccgacgatc
     2161 acggagtcgc ctggctccac cagattgacc acagcggcct ccatcccggc ggaaccggtt
     2221 cccgaaacgg cgatcgtaaa acggttggta gtctgaaaga cgttgcggag caacgtttgg
     2281 atatcgttca ttaccgtaag aaacgccggg tcaaggtggc ccaacagcgg ggtcgccagt
     2341 gcccgtaaga ctcgaggatg gacctgactt gggcctgggc cgagtaagag gcgttgtggg
     2401 ggcagaaatt catgcatgct caagggctcc gatcgggatt tcggcgcagt atagcgaaat
     2461 tcagtcatct tcggccatgg ggttcttgga acttgtactc acaaagcaac catttcgagg
     2521 ggtttcgggc ctgaaaaccg ccatgttata gttcatccac gtatggaatc tgaaacgaca
     2581 gcttcccctc cttcattatc gacctcttca tggtggtttg aagtcaatcc gcctccggcg
     2641 gttggcgact actccaaccg attttctccg atcgtgagcg tgctgaccgc gttgtttctc
     2701 gtggctgcct tgagttccgt ggtctggttg tcgtcatcca ttccaaagct cgaacgggtg
     2761 gaagcgccgg agcgggcact cgcgctcatg gtaagccgca tcatggatgt gcaggaagga
     2821 atgaagcggg cgccaacatg gcaacaaacc ctgtttggct ggatttccgg tgataatgag
     2881 ctcgaacggg cacaggcgat cgcatggttt caggaacttg cggagatgtc ggacaatgcg
     2941 ctcgtgccct tgcagttggc ggtcctacag gccgagtccg gacacgtctc tcaagcgctc
     3001 ctcttagccc atgagtggag tcaagcggtc gaaccgctcc cacaattggc gagtctggtc
     3061 gtcgcggcct atggagaggg accacttcct agtggcgaaa cattcgaggc cttgcaggcg
     3121 gagttggccg agttagtacc gaccggctgg ttctacaacc gactggcgga gcgtctcgcc
     3181 caacgagcca acaatcaacc gctcttggac acgattcgcg aggcctatgc cccccgtgtc
     3241 gatcgacagt ttgcccgtgc gcgagcgatt accgccctcg agttcacggc gatgatcgtg
     3301 gggacggtcg ttctcgtcat cttctggatc actagggcac gggtcccaaa tgtcgtgagg
     3361 cttcacgaac cgggtgttcc gccgccgtgg cctggtggga tcggcgctgc ggtcctcctc
     3421 cgcggagggg ctatcggggc gatgctcacc gtcgtgtttc ttctttacat gccgcaagaa
     3481 aacgcatccc ttcgagcact ggcgattcca ctgacgaact tgccgttgct gctgttggcc
     3541 taccagtatc tttttaagcc ggcccagatg acgttcgacc aggggttcgg attggagatc
     3601 ggatgggccc gtgccggtcg actggtcgcg gcagtactcg cggtcatggc ggctgggttg
     3661 tggggtgaat ggatcatgga tcgaatggct gaaccgctgc acctgacaaa ccattggacg
     3721 gagtggttcg acgccgattt ggtctggggc ccctcctcgt tgacggccat cagcctcgtg
     3781 gaatacgtgg ccttcgcgcc ggtctttgaa gaattggcgt ttcgcgggtt gctgttcgcc
     3841 attctccgcc gaaagtttcg cttccttccg gccgcgctca tgagcgcgag cgtcttcgcc
     3901 attgcccacg gctacgggct cgtcggcttc gtcagcgtgt tgtggagcgg gctgctgtgg
     3961 gcctggatct atgagaaaac tgggagcctg cttcctggca ttctcgcgca tgccataaac
     4021 aatctgctgg tgtgtctgag cgtcatggcg cttctgcgct ggtagcggaa gataaaatta
     4081 acaatcagtt cgaccgccgg agtgacacta ccgccgcgtg ccctccaact gttccaattc
     4141 agcatagaga tctgggtgtc gaagggtcgc cccgagcttt tctcgaagcg cgatcgtttt
     4201 gatgcgcttg cctggtcggt tgtcgttcgc acccgcgtga attgcaacat cccagcgtgt
     4261 ctgtgccact cggcagatgt ccgcccatgt gtgcggcgtt ccatcgctga cgttgtacac
     4321 ctcgcccggt gtccctgtct caagtgcgag catgcagatc gcggccagat cttcgacgtg
     4381 aatcaggttc acatattttc tggtcggcct gacacgtcca cttctgatcc actcgatcgg
     4441 attccgcccc gggccgtaga tgcctgcgac gcgaagcacg atcgcgccat gttcttttcg
     4501 aagatattct tctccccgca cgcgcggctt gcgcagatcg accggcgctg attcgtcaat
     4561 ccagggcggc ggataatcgg ttgattgact cggtcgatcg taagcggacg tgctgcctaa
     4621 gacgacaagg cgacgagggg gcacgccaag ccccttcgcg aaagcccgaa cctgttcgag
     4681 cggcgtggcg gggaaacacc agactatatc ggtattggtg ggaatgttcg tccacgtgtc
     4741 aggctgcgca agatcgaacc tgagtcgttg cggcggaggg acatgcgtca gccggcgatc
     4801 agggtcacgg ctggtggata ggacgatcct actctggcag caggcttcgg cgatcagaaa
     4861 acggccgaca tagcctgatc ccaacacagt gagcggcaat ctcttctgac gtagcacgga
     4921 ggcagttgcc ctttcggttt cccatttttt catacttgcg cgagatcaca caattcttgc
     4981 gttcgaggag gggtcataag agaatgcgcg atatgaagtc cattgcatcc cttatgacca
     5041 tcgccggcct tgcggtgacc ctttcaggct gcccagatag tgggggtgga ggcggtaatg
     5101 cctcaaacct tgccatcacc acgacgtcgg ccaactttgc cgtggcgggg aatccctatt
     5161 catcgacgct ggctgcgacg ggtggaaccc ctccgtacac gtgggcagcc gtgagtgtat
     5221 tgcctggttg ggtcttactc aatccgatga caggagtttt gacgggaacg ccgggaactt
     5281 cggatgtcgg caattttaat ctgacttttc gggtgaagga taccacggga gcgactgcgg
     5341 tcggaacggt tctcctggcc gtgcacaatc ggaccgatat attgtcggtt gacaatagta
     5401 cacctccagt ccctggggga gcagccagca gcagtccatc gatcagcaac gacggtcgtt
     5461 tcgtggcctt cgtctcaacg gccaacttga tccccggcgt cggcgggtcg caaatttacc
     5521 tccatgactg gcagacgaat caaacatccc tcatctctcg tgacagcggt gtggcgatca
     5581 acgctggcaa tgggataagc agtgcgcctt cgatcagctc agacggccat tttgtggcct
     5641 tcgtctcgaa ctcaacaaat ctagtatccg gtgtgagtgg ccagcagatt tatctgcgca
     5701 acgtccaaac cggcgtgaca actctggtgt cgaaagacaa tttaaataat ccagccaccc
     5761 tcggatcgac cacaacatca ccaagcatca gcagtactgg gcgttttatc gcgtatgtct
     5821 caaacgccac gaaccttgtt gcgggggtgc ttggcggtca acaaatttat ttatacgata
     5881 cccaaatcac gatggccttt cccaacggac aaacgacgct cgtttccaag gacaatagcc
     5941 ccacgccgtt gcctggagat ggagcgagca gtgcgccatc catcagtcag gacggctgtt
     6001 tcgtggcgtt cgcctccttg tcgacgaatt tgatttctcc atcccctgga aatcaacaag
     6061 tctatgtccg gggtcctcta ccgggtccag ggacctgtgg cgcaccagag caaacaagtc
     6121 tcatgtcgca ggacaacagc gcatcaccat tgccgggcaa cggcttcagt agcagcccct
     6181 ccatcaattc ggatggacgc ttcgttacct ttacttcggc ctcgagcaac ttattgacag
     6241 gtgtgagtgg gcagcagata tatcttcacg atcgcaatag cgggccgaac ggaacaacaa
     6301 gcctcgtatc aaaagacaac tcagcgctac cggtccaggg gaatggcagt agcagtgtcc
     6361 cgtccatcag tcaggacggc tgtttcgtgg cgttcgcctc tttgtcgacg aatttcggtg
     6421 tcgcaggcgg caaccagcag atctatatcc ggggtcctct gcctggccca ggaacctgtg
     6481 gtgtgcccga gctgaccagt cttgtgtcga aagacaacag cgtggttccg gtggctggaa
     6541 atgcagcaag tgatgccccg tcggtcaatg ccaacggtgg gttcgtggcc ttctcatcgt
     6601 tgtcgaccaa cttcggcgtc gcaggtggga accagcaaat ctttgtccgc gccatgccgt
     6661 aacatcggta gagcttcctg tcgcagcgtg gtacgatcac gccgcgatgc cactcacgct
     6721 ttttcacccc ctcattgccg aatggttcag ctcatctgtt ggagttccga ccgatgtgca
     6781 gcaagccgcc tggcctgcga ttcagtctgg tcaagacgcg ctcattgccg cgccgaccgg
     6841 atccgggaaa accttcgccg cgtttctctc ctgtatcgac cacctgttca agcaagctct
     6901 cgcccgcgaa ctcgatgatc acacgcaggt actctacgtt tcaccgctca aggctctgag
     6961 caacgacgtt caaaaaaacc tgcaacagcc gttaaatgag atcgggcagg cggcgttgca
     7021 agccggcttc ctgatgcctg aattgcgtgt gctggtgcgc accggcgata cgccgatggt
     7081 ggatcgccag cagatgctga agcggccgcc acacatactc gtgaccacgc cagaatcgct
     7141 ctttatccta ctcacggctg agaagagccg acgaatgctc cagacggtcc ggaccgtcat
     7201 cgtggatgag atccatgccg tcgctccaaa caagcgagga gcccatctgg ctctgtcatt
     7261 agagcgattg gaggcgctga cgttgaacaa accgcaacgg atcggcctgt ctgcgaccca
     7321 gcggccgatc gaaaccgtcg cgcaattcct tgtgggtgat cgaccgatgc cgaccatcat
     7381 tgacgtgggt cacaagcgag cgatggatgt ggccgtggaa gtgccaaaag atgaactcag
     7441 tgcggttgcg acgaacgcga tctggaccga tgtctacgat cgattggccg aattgatcag
     7501 gcagcatcgt tcgacgctcg tgttcgtcaa taccagacgg ttagctgaac gggtctcgca
     7561 ccatctcgaa gagcgattgc aggaccttgg cgccgatgct gtggcggcgc atcacggcag
     7621 tttgtcgcgc cagatcaggc tctcggcgga agagcggctg aagttgggca agacgcgggt
     7681 ggtggtggcg accgcgtcgc tcgaattggg catcgacatc ggaaccgtcg atctggtctg
     7741 ccagctcggc tcgccgcgcg ccatcgccac ctgtctccaa cggattggac gcgccggtca
     7801 ctggatcaag gcgataccca aaggccggtt gtttgcactc accagagacg agctccttga
     7861 atgcgccgcc ctggtgcgag cgatccgcag gggcgtgctc gatcacatca gtgtgccacc
     7921 tgcgccgctc gatgtgttgg cgcaacaagt ggtggcggcc gccgcatccc aaacctggca
//
LOCUS       DATDNW010000028         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_890650, whole genome shotgun sequence.
ACCESSION   DATDNW010000028 DATDNW010000000
VERSION     DATDNW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890650"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VI359_00705"
     CDS             <1..197
                     /locus_tag="VI359_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6972798.1"
                     /translation="TPASMVGRILGLYSTGAMIAAISGISTFGWVTERFGESTGVSGI
                     GATFLLTASLGGWLSRSIRD"
     gene            210..>2045
                     /locus_tag="VI359_00710"
     CDS             210..>2045
                     /locus_tag="VI359_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY6972799.1"
                     /translation="MEELSCQTDQYNIVASTSLTDDHMKVLKQGDTFGVFDRGGDIRC
                     ARQGPQGLYHEGTRFLSRFELTINGDQPLLLSSTVKQDNVLLSVDLTNPDMTREGEVA
                     ITRGSLHLSRSRFLWQGLCFERLRIHNYSLLPIPVRLAFTMDADFADLFEVRGKKRDR
                     RGRRLETITSKDGLSLGYEGLDRVVRRVTVRCSPAPTVIHPDELQCETLLPAGEAIQW
                     DLTISCEVDRQPTCELVYEKALVEVQRALQSAKADDCLIVTSNEQFNAWLNSSLADLH
                     MMISDTPEGPYPYAGVPWFSTPFGRDGLITAFELLWINPTIARGVLAYLAATQAREAA
                     PTQDAEIGKILHETRKGEMAALNEIPFGRYYGSIDATPLFIMLAGAYYERTGDQIFIE
                     QIWPNIELALQWIDSSGDLDGDGFVEYRRQSNGGLAHQGWKDSQDAVFHADGNPAEAP
                     IALCEVQGYVYAAKRRAARLALALGHPERAKPLIKEADKLRYRFERSFWSNDLSSYVL
                     ALDRFKQPCRVRTSNAGHCLWTGIADQKHGLKTAKTLVSEDSFNGWGVRTVAATEIRY
                     NPMSYHNGSVWPHDTALIAAGMASYGFKQGALKMLSGLFDASLFLD"
BASE COUNT          437 a          546 c          599 g          463 t
ORIGIN      
        1 tgacgcctgc ctcgatggta gggcgtatcc tgggcctcta cagcacgggt gcgatgattg
       61 ccgcgatcag cgggatcagt acattcggtt gggtgacgga acggtttggg gaatcgaccg
      121 gcgtttcagg cattggcgcg acatttctcc tgactgcttc tctcggaggt tggttgagcc
      181 ggtcgatccg agactaaagg gagggacttg tggaagaact cagttgccag actgatcaat
      241 acaatatcgt ggcctctact tccttgactg atgaccatat gaaggttttg aagcagggtg
      301 atacgttcgg tgtgtttgat cggggcggtg acatccgctg cgccaggcag gggccgcagg
      361 gcctttacca cgaaggaacc cgatttctgt cccgtttcga gctgaccatc aatggagatc
      421 agccgttgct gttgagctcg acggtcaagc aagacaacgt gctgctgagc gtcgatctca
      481 caaatcccga catgactcgt gagggggagg tggcaatcac gcgtggttcg ctacacctgt
      541 ctcgatctcg attcttgtgg caaggcctct gtttcgagcg ccttcgaatt cacaactata
      601 gcctgcttcc aatcccggtt cgacttgcct ttacgatgga tgcggatttt gccgacctct
      661 tcgaagtccg tggaaagaag cgagaccggc gcggccgacg gttggagact atcacgtcca
      721 aggacggact ttcgctcggg tatgaaggct tggatcgtgt ggttcggcgg gtcaccgtcc
      781 gctgttcacc ggcacccacg gtcattcatc ctgatgaact acagtgcgag acgcttcttc
      841 cggctggaga ggcaatccag tgggatctca cgatttcatg tgaagtggac cggcaaccca
      901 catgcgagct cgtatatgag aaggcactcg tcgaggtaca acgggctctc caatccgcca
      961 aagccgacga ttgtctcatc gtgacatcaa atgagcagtt caatgcctgg ctgaacagtt
     1021 cgctggccga ccttcatatg atgatctccg atacgcccga gggtccatac ccgtatgccg
     1081 gggtcccatg gttcagcacc ccgtttggac gcgacggcct catcaccgca ttcgaactgc
     1141 tgtggatcaa tccgaccatc gctcggggcg tattggctta tttggcggct acccaggcga
     1201 gagaagctgc gcccacacag gatgcggaga tcggtaagat tctccatgaa actcgcaagg
     1261 gagagatggc ggccctcaac gagattcctt tcggccgcta ctacggcagt attgatgcga
     1321 caccactgtt tatcatgctg gctggagcat attacgaacg taccggggat caaatattca
     1381 tcgagcagat ctggccgaat atcgaactgg cgcttcagtg gatcgacagc tcgggagatc
     1441 tcgacggtga cgggttcgtg gagtatcgcc gtcaatcgaa cggcgggctt gctcaccaag
     1501 gatggaaaga ttcgcaggat gccgtctttc acgctgatgg taacccggct gaagcgccca
     1561 tcgcactctg cgaagtacag gggtatgtgt atgcggccaa gcggcgagcg gctcgattag
     1621 ccttggctct gggacatccg gagcgggcca agcctctcat caaggaagct gacaaattgc
     1681 gttatcgatt cgagcggagt ttttggagta acgacctctc atcatatgtt ttagcattag
     1741 accggttcaa gcagccctgt cgggtcagaa cgtcgaatgc cggtcactgc ctgtggaccg
     1801 gtattgccga ccaaaagcat gggctgaaga cagccaagac cttagtaagt gaagattcct
     1861 ttaacggatg gggagtccga accgtagcag ccacggagat tcgctacaat ccgatgtcct
     1921 accacaacgg gtccgtctgg ccgcacgata ctgctctgat cgcggccggt atggcttcct
     1981 acggcttcaa gcaaggtgca ttgaagatgc tcagcgggct tttcgacgcc agcctgtttc
     2041 tcgac
//
LOCUS       DATDNW010000029         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1300216, whole genome shotgun sequence.
ACCESSION   DATDNW010000029 DATDNW010000000
VERSION     DATDNW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300216"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..640)
                     /locus_tag="VI359_00715"
     CDS             complement(2..640)
                     /locus_tag="VI359_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972800.1"
                     /translation="MTLTSILYSLILILVSVTWALEPSSRPLVDVTDPAALQEATRVL
                     AEEVKVAARPQTYVLIDLAANTILLKGHGVELHRISVRNWTTSDQGELATTFRLRERP
                     PVTRRKIDPSISSERDPISLADMPTAYSLRFTPALMITIDSYDHASVWGMLSHFMSQW
                     WRLIVKWWHALWTEAGSPATPELDLTMSVDDAQSLAWMLTDGMPVLIRRPTQ"
     gene            complement(666..1301)
                     /locus_tag="VI359_00720"
     CDS             complement(666..1301)
                     /locus_tag="VI359_00720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY6972801.1"
                     /translation="MKQATRALYLGVIGLLFLLTLAQGSEIPSPGTPTVPSKPADPAA
                     LRQLQYRYHSLSKQLVQLMPAQPYILVDTARNHLYIRHRDRVMLDALASTGSGTMLDK
                     PGETQSQWIFDTPRGEFFVQSKLTNPVWVKPDWAFIEEGLAVPKNQADRIEPGVLGEY
                     ALGFGKGYFIHGTLYTRLLGKNVTHGCIRLNDEDLKSVYRLAKVGTPIIIF"
     gene            complement(1311..2456)
                     /locus_tag="VI359_00725"
     CDS             complement(1311..2456)
                     /locus_tag="VI359_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY6972802.1"
                     /translation="MMKYRRLMYSFARGCAFLVLAVVLTACTDAVPQDVVDAIESIDR
                     DLMDLRAAELAPDDYSRFAHQWMALKARVQAEEDLIHWPWESNELDLALRGLYEEGVQ
                     TVARVTDQRKALRQTAEHKLASVEDRFQILTDQVGAIDSRLVLGQQLVETDLLVKQAR
                     AFFEEGQFERSIEASDKAGQMLTSQTAVLGSELGRYADHGRIGRWQQMVKETIEWSRR
                     HRTSAIVVSKADRVLTLYKNGQKVLSYPVRLGFNGIREKRYQGDGATPEGRYRITGKR
                     GQGQTQFYRALLLDYPNEEDRRRFKSARKTGLIPASKGIGGQIEIHGVENELMAQTLG
                     CVMLENAQMVYLYDRVEKGTPVTIVGALHEHNSVAQALASLGQRNNI"
BASE COUNT          524 a          683 c          712 g          562 t
ORIGIN      
        1 cctattgggt tgggcggcga ataagcaccg gcatgccatc tgttagcatc caggccaacg
       61 attgtgcatc gtcgacgctc atggtgaggt caagttcggg cgtggcggga gaaccagctt
      121 cagtccagag tgcatgccac catttgacga tcagtctcca ccattggctc ataaagtgtg
      181 acagcatccc ccagacacta gcatggtcgt aggaatcgat tgtgatcatc aaggccggag
      241 taaaccgcag agaataggcc gtcggcatgt cagccagaga gatcgggtcc cgttcagaac
      301 tgattgaagg gtcgatcttc cgacgggtga caggagggcg ttcccgcagt cgaaaggtcg
      361 tggcgagttc gccctgatcg gatgtggtcc agttcctgac cgaaatccgg tggagttcta
      421 cgccgtggcc tttgagtagg atggtattcg cagcgagatc gatgagcacg taggtctggg
      481 gtcgagcggc taccttcacc tcttcggcaa ggacccgcgt ggcttcttgg agagccgctg
      541 gatcggtgac atccacaagc ggtcgcgacg agggttcaag agcccatgtc accgaaacca
      601 gaatcagaat gagagaatag agaatggagg tgagcgtcat ggaggctgcg tcgggccgca
      661 gctggctaga aaattatgat cggcgtaccg acctttgcaa gtcgatagac actcttgaga
      721 tcctcgtcat tgagacggat acacccgtgg gtcacgttct tcccgagcag cctggtatag
      781 agcgtgccat ggatgaaata gcccttgccg aacccgagcg catactctcc cagcacgcct
      841 ggctcgatgc gatctgcttg gttcttgggg acggcaagac cttcttcaat aaaggcccag
      901 tcaggcttca cccacacagg attcgttaat ttcgattgga cgaagaactc gcctcgcggt
      961 gtatcaaaga tccattggct ctgagtctca ccgggcttgt ccaacatggt accactcccg
     1021 gtcgaggcca gcgcatcgag catgacacgg tcccggtgtc ggatatacag gtggtttcgg
     1081 gccgtatcga ccaggatata gggttgagcc ggcattaatt gaacgagttg cttgctgagc
     1141 gaatggtaac ggtattggag ctgccgtaag gcagctggat cagctggctt agagggaaca
     1201 gttggggtac ctggcgacgg gatttcgctg ccttgggcca aagtgaggag aaacagcagg
     1261 ccgatcactc caaggtacag tgcccgcgtc gcctgtttca tggggcttac tcagatattg
     1321 ttccgctgcc cgagactggc taaggcttgg gcaactgaat tgtgctcgtg aagggctccg
     1381 acgattgtca cgggagtccc cttttctact cggtcataca gatacaccat ctgggcattt
     1441 tcaagcatga cacagcccaa tgtttgcgcc atcagctcgt tctcgactcc atggatctcg
     1501 atctgtccac cgattccttt cgatgctgga atcagtccgg tcttccgtgc cgatttgaac
     1561 cggcggcgat cctcttcatt ggggtaatcg agcagcaagg cccgataaaa ctgagtttgc
     1621 ccttggccgc gcttgcccgt gatgcggtaa cggccttccg gcgtggcgcc gtctccctgg
     1681 tatcgcttct cacgaattcc attgaaccct agccggacgg ggtatgatag caccttctgt
     1741 ccgttcttgt agagggtgag cacacggtcg gctttgctca caacgattgc actcgtgcgg
     1801 tgacgacgag accactcgat cgtctccttc accatctgtt gccatcgacc gatccggccg
     1861 tgatcggcgt atcgtcctaa ctcactgcct aacacagcgg tctgggaggt gagcatctgt
     1921 ccagccttgt cagatgcctc gattgatcgc tcgaattgcc cctcttcaaa gaacgctctt
     1981 gcctgcttta cgaggaggtc ggtctcaacc aactgctggc caaggacaag tcggctatca
     2041 atcgctccaa cctggtcagt cagaatctgg aaacgatcct cgacagaggc aagcttgtgt
     2101 tcagcagtct gacggagcgc cttgcgttga tctgtgaccc gtgcaacggt ctggacccct
     2161 tcttcgtaga gtccgcgaag ggccaagtcg agctcattgg attcccatgg ccagtggatg
     2221 agatcttctt cggcctgaac acgagctttg agggccatcc attgatgggc aaatcggcta
     2281 tagtcatccg gtgccagttc cgcggcccgc aggtccatga ggtctcgatc gatcgattcg
     2341 atcgcatcga ccacgtcttg aggcacggca tccgtgcagg cggtcaagac gaccgccaag
     2401 acgaggaacg cacaaccacg cgcgaacgaa tacatgagcc gtcgatactt catcatgggc
     2461 tgcctggctt acttcttttt c
//
LOCUS       DATDNW010000030         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_685844, whole genome shotgun sequence.
ACCESSION   DATDNW010000030 DATDNW010000000
VERSION     DATDNW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685844"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VI359_00730"
     CDS             <1..539
                     /locus_tag="VI359_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6972803.1"
                     /translation="PLLHDGRTIGVSMRRYSPQTEMLNSMKGFTLMEVMIVAAIIGLL
                     SAIAVPNYLQWQSRYQLKQAVMEIASELNTSRFLAMNRNAAITTTFAISGGQLTTLAT
                     DPSGNPLITQATNLPKITNLVLVGGGTLQIGSSGLRASGTPGSDQLIQVTNDVGKIYS
                     VRLTQGGKASWCPKDTCP"
     gene            567..1055
                     /locus_tag="VI359_00735"
     CDS             567..1055
                     /locus_tag="VI359_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6972804.1"
                     /translation="MRTESGFTLIEAMMASVILAVGLLALSFMQATTLSRNVDSAELT
                     RATSLASEMVERIQYNRTRVTAYNGITSAGAGNCNTISATNDPMARGDCNQWVALLGG
                     PYGAGLNGLVGTVSVTATGPTIPPLNQSLVAVQVTWTTTAGVNKAARTRQVVINTTID
                     PE"
     gene            1068..2192
                     /locus_tag="VI359_00740"
     CDS             1068..2192
                     /locus_tag="VI359_00740"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY6972805.1"
                     /translation="MIEEMKMVGSWRSQRGATLIELLIAVFVALVITASGFAILTSTE
                     KSTRTNEQVVETQQNARIAMDLLSRDIKNAGFGMIAPLADNNCKVGRAASGAAAALVP
                     WDQTVAGADKGPDEISMVVPLGSAGTPAWTLTNPVGGPAGFNQITLPAGAAANMASQA
                     GGSLTVPTAAYLSIGGAATSTVTGVAGDTITLAPLPGPLAFGTGAKVYLLQCVTYSIG
                     TTPAACGNGSSPCLRRNGAVIADGIEDIQFEYACDGCNSGVNGGVVDGIIDDQVTLGN
                     GYDSGDFVSNNAWTASPMTPETIRMVKVFIVARQTIADTGMGETSGHGVITSAPLQVS
                     DHDHSQGVFASGDFAALDPPYQRFRRRVLTRTIEARNLAH"
     gene            2207..2863
                     /locus_tag="VI359_00745"
     CDS             2207..2863
                     /locus_tag="VI359_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972806.1"
                     /translation="MKQLVAKSGGETSGNECGIALLTVMLLLLILTVLGVAAITVTGL
                     EIRMAGFSSSTEGAVAAAESCAGTGVNIIAQTLSLGAVPTQYVVGGSGATSPVIPANA
                     ENASTNPPSLTQEIFGQANNDGDISTGASSKPDLSFTVGSYAVVGDIDRLYVTQKAGS
                     GLQYAGGYGGPGQGGGGSQDIFYRLDCQATNVATGTTNRVQAVYACLNMGETCQKKSF
                     "
     gene            2863..3276
                     /locus_tag="VI359_00750"
     CDS             2863..3276
                     /locus_tag="VI359_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972807.1"
                     /translation="MGNLVDGKHLTVSGHHKEKPMDTRNSSGRPQVGLWLYGLVLCLL
                     FALSSPGMAQVNPADDLPGGIWGKLEFLTNHDAVVDGRRYPYAHNVIIHTRALKPDKR
                     GNVKVVLDQKGYAIEISLYGIEEVDAMTKYFKHEG"
BASE COUNT          818 a          842 c          991 g          703 t
ORIGIN      
        1 gtcccttgct ccatgatggc aggaccattg gtgtatctat gcgacgatat tctcctcaaa
       61 cagaaatgtt gaactcgatg aaggggttca cgctgatgga agtgatgatt gtggcggcaa
      121 tcatcggact tctcagcgcg atcgcggttc cgaattatct gcaatggcag tctcgctatc
      181 aattgaagca agcagttatg gagattgcca gcgagctgaa caccagtcga tttctcgcga
      241 tgaatcgcaa cgctgcaatt acgacaacct tcgcgatttc cggtggacag ctgaccactc
      301 ttgcaacaga cccaagcggt aacccgctca ttacacaggc gaccaacctt ccgaagatca
      361 cgaacttagt tctggtgggg ggagggaccc tccagatcgg ttcatccggt ctccgggcga
      421 gcgggactcc cgggtctgat caattgattc aggtgacgaa cgacgtgggg aaaatatatt
      481 cagtgcgatt aacccagggc gggaaggcaa gctggtgtcc gaaagacact tgcccgtagg
      541 cgtacgagag taaccaggag atgcgagtgc gaacagaatc aggctttact ctgattgaag
      601 cgatgatggc atcggtcatt ctcgcggtcg gactcctcgc tctgtcgttt atgcaagcga
      661 caacactgag ccgaaatgtg gattcggccg agctaacccg cgcgacaagt ctcgcctcgg
      721 aaatggtcga gcgaatccaa tacaatcgca cgcgagtcac ggcctataac ggaattacca
      781 gcgcaggggc gggaaactgt aacacgatca gcgcaacaaa cgatccgatg gcccgaggtg
      841 actgcaatca gtgggtggct cttctgggtg gcccgtatgg ggcggggctc aacggactgg
      901 tcggaacggt cagcgtcacg gcgactggcc caacaattcc tcccctgaac caaagtctcg
      961 tcgcagttca ggttacctgg acgacaacgg cgggcgtgaa caaggcagca cgaacacggc
     1021 aagtggtgat caacacgacc atcgatcctg aataaaaggc catagcgatg attgaagaga
     1081 tgaagatggt gggttcatgg agaagtcagc gaggggcgac gctcatcgag cttttgatcg
     1141 ccgtctttgt ggccttggtc atcacggcct caggatttgc cattctcacg tcgactgaaa
     1201 aatctacgcg aacgaacgag caggtggttg aaacgcaaca gaacgccaga atcgcgatgg
     1261 acttgctgtc ccgcgatatc aagaacgcag ggttcggaat gatagcacct ctggccgaca
     1321 acaattgcaa ggtaggacga gcggcttcag gagccgccgc agcacttgtg ccgtgggacc
     1381 aaaccgttgc cggagccgat aaaggaccgg atgagatttc aatggtcgtg ccgttgggaa
     1441 gcgcgggaac cccagcctgg acgctcacga acccagttgg aggtcccgcc ggattcaatc
     1501 aaatcacgct tcccgccgga gcagcagcca acatggcgag tcaagcggga ggcagcctca
     1561 ccgtaccaac tgctgcatat ctttcgattg gaggagcagc gacgagtact gtgacgggag
     1621 tggccggaga cacgattacg ctcgcccccc taccggggcc actcgccttc ggcactggtg
     1681 ccaaagtcta tctactgcag tgcgtcacat actcaattgg gacgacaccg gcggcctgcg
     1741 gaaatggcag cagtccctgt ttacgacgta acggagcggt cattgccgat ggaattgaag
     1801 acatccagtt tgaatatgcc tgtgacggtt gcaactccgg ggtcaatggg ggggttgtag
     1861 acgggattat tgatgatcag gtgacgcttg gaaatggcta cgatagcggt gactttgtct
     1921 caaacaatgc atggaccgcc tctcccatga cgcctgaaac tattcggatg gtcaaggttt
     1981 tcattgtggc gagacaaacg attgccgata ccggaatggg ggagaccagc ggacacggtg
     2041 tgatcactag tgctcccctc caggtcagtg atcacgacca ttcgcagggc gtcttcgcat
     2101 caggcgactt cgccgcgctg gatccaccat atcagcgatt taggcgacgg gttctgacaa
     2161 gaaccattga agccaggaat cttgctcact agggagtgta actgagatga aacagcttgt
     2221 ggcgaaatca ggtggcgaaa catcggggaa tgaatgcggc atcgctcttc tgacagtgat
     2281 gctgctgctg ctgatactga ccgtgctcgg cgtggcagcc atcacagtga cgggcttaga
     2341 gattcggatg gctggatttt ctagttcaac ggaaggcgcc gtggcggctg ctgaatcctg
     2401 tgccggcaca ggggtgaaca tcatcgcgca aactctgagt ctcggggctg taccgaccca
     2461 atatgtggta ggaggatcgg gtgcaacgag tccagtcata ccagccaacg ccgaaaatgc
     2521 ttcgacgaat cctccgtctc tgactcaaga aatttttggc caggccaaca atgacgggga
     2581 tatttctacc ggagcgtcgt cgaaaccgga tctttcgttc acggtagggt cctatgctgt
     2641 cgtgggggat attgatcggc tgtatgtcac acaaaaggca gggtctggtc ttcaatatgc
     2701 tggaggatat ggagggcctg ggcagggcgg tggaggatcg caagacatct tctaccgact
     2761 tgattgtcag gcgaccaatg tcgcgacggg gaccacgaat cgggttcagg cggtctatgc
     2821 ttgcctgaac atgggcgaaa cttgtcagaa gaaaagcttc tgatggggaa tcttgtggat
     2881 ggaaaacacc tgacggtgtc tggacatcat aaggagaaac cgatggacac gagaaactcc
     2941 tcaggcagac cacaagttgg actctggctg tatggcttgg tgttgtgtct cctattcgcc
     3001 ttgtcctcgc caggcatggc acaagttaat ccagcagacg acctaccagg cggcatctgg
     3061 ggaaaacttg aatttctcac caatcacgat gcggttgtag atgggcgtcg gtatccctat
     3121 gcacacaatg tgattatcca tactcgtgct ctcaagcctg acaaacgcgg aaacgtgaag
     3181 gtggtattgg atcaaaaagg atatgcgatc gagatcagtc tctacggcat cgaggaagtg
     3241 gatgccatga cgaagtattt caaacatgag ggctaacgtg aggaggagta ccatgaaaca
     3301 gatcctcggg gggctcttat tggcaggaat tctgctcgtc ctgggtcagc atgc
//
LOCUS       DATDNW010000031         7463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_276164, whole genome shotgun sequence.
ACCESSION   DATDNW010000031 DATDNW010000000
VERSION     DATDNW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276164"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VI359_00755"
     CDS             <1..597
                     /locus_tag="VI359_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HEY6972808.1"
                     /translation="RDRKERDVAAAFLVDVSGSTSRQLDGGRRVIDVEREGLVLLCEA
                     LEAVGDQYALYGYSGQGRGHVDFLVIKDFADRLGGQAAHRLAGLMPLQQNRDGAAIRH
                     ATTKLLAREAKTKLLIVVSDGRPLDDGYKDEYSLEDTKAALREAKRRGIHPFCITIDR
                     DADAYLKRMYGDVQFTVIDRVETLPLRLPRIYQRLTTA"
     gene            594..1001
                     /locus_tag="VI359_00760"
     CDS             594..1001
                     /locus_tag="VI359_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972809.1"
                     /translation="MTELSGKPTVPPWLYKLFTGHQYPYVRRLAKFAQPVKPGEDRLE
                     PTKEMIEAKFWEVYPRCSVKILQEVKVGMIVVFHDLGEYPAGGYQELVDNPEEFLAKT
                     YGKKKIKVNFYDGENFVCTINFKVAGWTEHAHS"
     gene            1086..2024
                     /gene="mdh"
                     /locus_tag="VI359_00765"
     CDS             1086..2024
                     /gene="mdh"
                     /locus_tag="VI359_00765"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY6972810.1"
                     /translation="MGRPKITVVGAGNVGGTTAQRLAERDAYDVVLVDIIEGVPQGKA
                     LDITQAGPVYGYSTRVVGTNGYDETAGSSIAVITSGIPRKPGMSRDELLTTNAKIVQS
                     VVKELISRSPGCILILVTNPLDAMVHVARKISGLPKSKVLGMAGVLDSARMRAFIAEE
                     LHVAGPDVEAMVLGGHGDTMVPLPRYTTVKGKPVTDLMAKERMEAIVKRTREGGAEIV
                     GLLKTGSAFYAPSASAVDMVDAIMKDQKRVLPCAVLCDGEYGLQNVIVGVPVKLGRGG
                     AEQIVEYELTVDERAALETSAKAVRELCATVDRLMA"
     gene            2139..2717
                     /locus_tag="VI359_00770"
     CDS             2139..2717
                     /locus_tag="VI359_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY6972811.1"
                     /translation="MKFLEDPFQTMGAGFALAVVLIIVYLGIFGIGAGDADWGGILLR
                     WIHFLAGITWIGLLYFFNLINAAFMKSLDGPTKNAVIPKLMPSALNWFRHGATVTVLA
                     GILLYIKMYMNGGTGAYALAIGGLLGIIMMANVHGIIWPNQKKIIAAVSAGGPPPAEM
                     AQWGRTALLASRINFMLSIPMLFFMGAGSHFK"
     gene            2944..4245
                     /gene="nuoF"
                     /locus_tag="VI359_00775"
     CDS             2944..4245
                     /gene="nuoF"
                     /locus_tag="VI359_00775"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY6972812.1"
                     /translation="MTATAEARVLQTLDGAPWDIDAYMKVGGYEAWKKCVTELSPAQV
                     VEELKKSGLRGRGGAGFPTGTKWDKVLNHRVKERYFVCNAGEHEPGTFKDRHLLKMIP
                     HQLIEGCLIASRTVQAKASFIYVNHEYQEERDNLKKALAQAKARGFLGKNVLGTGVDI
                     DLEVFEGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCN
                     VPRILHKGAAWFTQVGTERCPGTMMFSLSGAVNRPGVYEMPMGVTVRELIDRCGGGVA
                     GGRKIKAVFPGGPAFSMITADQLDLPMDFDSLKKAGTGLGSAGVIVVDDATCMVAQTL
                     KFSNFFKEESCGQCPPCRMGTINLASLMTKIEQGEGTQKDLDSLLQLCGFVKGTGYCT
                     LVTGAAVLVQSSIKLFRHEFEEHIRLQRCPYQAVAVGVSTH"
     gene            4255..4602
                     /locus_tag="VI359_00780"
     CDS             4255..4602
                     /locus_tag="VI359_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6972813.1"
                     /translation="MPHVTFLHPEGKSGVVTRNLTLLDAAKELGFPLNHDCGGNASCT
                     TCRVEVQAGGEHLSEIEFDEQDLLDREALREPWHRLGCQAKVLGDVVVRVPETKWAAP
                     ATGNSEAQGVDIR"
     gene            4670..4990
                     /gene="erpA"
                     /locus_tag="VI359_00785"
     CDS             4670..4990
                     /gene="erpA"
                     /locus_tag="VI359_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY6972814.1"
                     /translation="MVTITTVAEQKIKELMTEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMSDDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSLQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            5120..5557
                     /locus_tag="VI359_00790"
     CDS             5120..5557
                     /locus_tag="VI359_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY6972815.1"
                     /translation="MAQASVTRRYRFCAAHRLHTDHLSPEENWAAFGKCNNPNGHGHN
                     YVVLVTVKSGLQSTGGKAANLAALDRVVTDTIITRFDHHDLNQDPEFADRTTTGENLV
                     KLIWDLLAVQLPIGSLERVGVVETRDNYFEYAGTTKPEGGSHG"
     gene            5550..6197
                     /gene="folE"
                     /locus_tag="VI359_00795"
     CDS             5550..6197
                     /gene="folE"
                     /locus_tag="VI359_00795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY6972816.1"
                     /translation="MAKLAVVRRRRQEGEDVSRSGRSPDLTLLQSLVTQMLLALGEKP
                     RRNGLMNTPERVAKALAFMTQGYQRNIDHLLNGALFPIEYDEMVIVKDIDFFSLCEHH
                     LLPFFGKVHVGYLPNKKVVGLSKIPRIVDTFARRLQVQERLTTQIAETLESKLSANGV
                     GVVIEARHLCMMMRGVEKQNTVAVTSSMLGTFRSQQQTRLEFLKLIRRGSVGDSD"
     gene            complement(6194..7015)
                     /locus_tag="VI359_00800"
     CDS             complement(6194..7015)
                     /locus_tag="VI359_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6972817.1"
                     /translation="MVRNLGVPMKAQINDITLAYADTGTGLPVVFLHAFPLNRTMWAA
                     QEEALSSRHRVITIDLRGHGESDAPLWHYTLEQAADDVRALLDHLSVPQAVFVGLSMG
                     GYILFAFYRKYADRVKGLILADTRAQADTPEGRTGRFQMAQIAYTKGPAAIADLMIPK
                     LLSPGTIETNPTLVQNVRSMIEGNQISGIAGDLMAMAERPDSVPLLRQITCPVQIIVG
                     ELDVVTPANDSRLMADQIPRAHLTTIPGAAHLSNLEQPEAFNQIVEAFVLTCAKT"
     gene            7111..>7463
                     /locus_tag="VI359_00805"
     CDS             7111..>7463
                     /locus_tag="VI359_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6972818.1"
                     /translation="MARDQHQPADLFTAPGTTPDTSISPLAARMRPQSFEDFIGQDEI
                     VGPDRPLRKAIEADRLSSIIFWGPPGCGKTTLAHLVARHTKAHFVSFSAVTSGIPELR
                     EVVRAAEHRLATSHQ"
BASE COUNT         1704 a         1889 c         2298 g         1572 t
ORIGIN      
        1 cgggatcgca aggagcgaga cgttgcggcg gcgtttcttg tggacgtaag cggttccact
       61 agtcgccaac ttgacggggg ccgtcgtgtc atcgatgtcg agcgagaggg gctggtgctg
      121 ttgtgcgaag cgctggaagc agttggtgat cagtatgcgc tctatgggta ttcaggccaa
      181 ggccgcggcc atgttgattt cttggtgatc aaggattttg ccgatcggtt gggcggacag
      241 gcggcgcacc gattagccgg gctgatgcct ctgcagcaaa atcgtgacgg ggcggcgatc
      301 cgccatgcga cgacgaagct gctggctcgt gaagccaaaa ccaagctgtt gatcgtcgtc
      361 agtgacggcc ggcccctgga tgacggctat aaagatgagt attcacttga ggacacgaag
      421 gccgccttgc gcgaagcgaa gcggcgaggg atccatccct tttgcatcac gatcgatcgc
      481 gacgccgacg cgtatctcaa acggatgtac ggcgacgtac aatttaccgt catcgatcgt
      541 gttgaaacgc tgccgttgcg gttaccgcgt atttatcaac ggctgacaac cgcatgaccg
      601 aattgtccgg taaacccacg gttccgccgt ggctctataa gctcttcact gggcatcagt
      661 acccttacgt ccgccggttg gcgaagttcg ctcagccggt caagccggga gaagatcgcc
      721 tggaacccac gaaggaaatg atcgaggcga agttctggga agtctaccct cgctgttccg
      781 tgaagatcct gcaggaggtg aaggttggga tgatcgtcgt gtttcacgat ctcggtgaat
      841 atccagccgg cggctatcaa gaactcgtcg acaacccgga agagtttctg gcgaaaacct
      901 atgggaagaa gaagatcaag gtgaatttct acgacggcga gaattttgtg tgcacgatta
      961 attttaaggt ggcgggatgg acggagcacg cacactcatg aatagtgctg agtgcttagg
     1021 ttcgcttgaa cgaggtgtga gggctgtctg ctcgggactc agcacttaga cctgtcggag
     1081 gaagtatggg gcgaccgaaa attaccgtgg tgggggctgg caatgtcgga ggcacgaccg
     1141 cgcagcggct agctgagagg gatgcctacg atgtggtgct cgtggacatt atcgaaggcg
     1201 tgccgcaagg aaaagccctg gacatcacgc aggccggacc ggtgtatgga tacagtactc
     1261 gtgtggttgg gaccaacggt tatgatgaaa cagccggctc gtcgatcgcc gtgattacct
     1321 cgggaattcc ccgcaagccg ggcatgagcc gagatgaact cttgaccacg aacgcgaaga
     1381 tcgtgcaatc ggttgtgaag gaactcatct ctcgctcgcc cggatgtatc ctgattcttg
     1441 tgacaaaccc tttggatgcg atggtgcacg tggcacgaaa aatcagcggg cttccgaaat
     1501 ccaaagtatt gggaatggcc ggcgtgctcg attcggcccg tatgcgcgcg tttattgccg
     1561 aggagttgca tgtcgcgggc ccggacgtgg aagccatggt gctcggcggc catggtgata
     1621 cgatggtccc gctgccccgg tatacgacgg tgaaggggaa acccgtgact gacttgatgg
     1681 cgaaagagcg gatggaggcg atcgtcaagc ggacgcgcga gggcggagcc gaaatcgtgg
     1741 ggcttctcaa gaccggtagc gcgttttatg cgccctctgc gtcggccgtc gatatggtgg
     1801 atgcgatcat gaaagaccag aaacgcgtac tgccttgtgc ggtgttgtgt gacggggaat
     1861 atggtttgca gaacgtgatc gtgggcgtac cggtgaaact tggacgaggc ggagcggaac
     1921 agatcgtgga gtatgaactg actgtagacg agcgggctgc ccttgaaacc tctgccaagg
     1981 ctgtccgcga actttgtgcc acggtcgatc gactaatggc atagatctgg agtgagagac
     2041 acggggagag caaacagagc tttcggcgat tgcttgacaa gagacgagag gctacagtac
     2101 agagtgcagc ctagcagtaa actgaacgag aggacaacat gaaatttctt gaagatccgt
     2161 ttcagacgat gggtgcgggg tttgcgctcg ccgttgtgtt gatcatcgtc tatcttggca
     2221 tctttggtat tggagcgggc gatgccgatt ggggtgggat tttgcttcgg tggattcact
     2281 ttctagccgg cattacctgg atcgggttgc tctatttctt caacctgatt aatgcggctt
     2341 tcatgaaaag tctcgatggg cccaccaaga atgccgtcat ccccaagctc atgccgtctg
     2401 cactcaattg gttccgccat ggggccactg tgactgtgct ggccggaatt ctactgtata
     2461 tcaagatgta tatgaacggt gggaccggtg cctatgcact ggcgatcggc gggttgctgg
     2521 gcattatcat gatggccaac gtgcacggca ttatttggcc caaccagaag aagatcatcg
     2581 cggctgtgtc ggccggtgga cctcctcctg ctgaaatggc ccaatggggg cggacagcgc
     2641 tgctcgcatc ccggatcaat ttcatgctct cgatccccat gttgtttttc atgggggcag
     2701 gcagtcactt caagtagtca acccatgaaa ccgggccggg agaagttcct cccgacccgg
     2761 ttctcggtct ttcaaatcca atctcttcga tcgctcggcc aatatgccca gcttgaccta
     2821 gggctgttgt cctaacctat tgagattcag gcctattcca gtccggttgc cttgacgaag
     2881 ggttaacggg cgccttatag taactagcca cggctgaact atctcgaagg atcgaacgat
     2941 attatgacgg cgactgctga agctcgggtt cttcaaacgc tcgatggagc tccctgggat
     3001 atcgacgcct acatgaaggt cggggggtat gaagcgtgga agaaatgtgt cacggaactg
     3061 agccctgcgc aggtcgttga ggaactcaag aagtccggtc tcagggggcg aggtggagct
     3121 gggtttccca caggaacgaa gtgggacaag gtcctcaatc acagggtgaa ggaacggtat
     3181 ttcgtctgta atgctggtga gcacgaaccg ggaacgttca aggaccgtca tctgttaaaa
     3241 atgattccac atcaacttat tgagggctgt ctcattgcct cgcgaaccgt gcaggcgaaa
     3301 gcttccttca tttatgtcaa ccacgagtat caggaggagc gagacaatct gaaaaaggcg
     3361 ctcgctcaag ccaaggcaag gggattcctc ggcaaaaacg tgctcggtac cggggtagat
     3421 atcgacctcg aagtcttcga ggggcacggg agttatgtgg ccggtgaaga aaccgccatg
     3481 ttggagtcta tgcagggccg gccggcgatg ccgcgacaga agccgccatt ctacccgacg
     3541 gacttcgggc tctatggcaa accaacgctc gtgaacaatg tcgagacatt gtgcaatgtc
     3601 ccgcggattc tccacaaagg ggccgcatgg tttacgcagg tagggacgga gcggtgcccc
     3661 ggaacgatga tgttctccct gagcggagca gtgaaccggc cgggagtgta cgaaatgccg
     3721 atgggtgtga cggttcgcga gctcattgac cggtgcggtg gtggggtggc gggtggccgg
     3781 aagatcaaag cagtcttccc gggtgggccg gcattttcga tgatcacggc cgaccaactt
     3841 gaccttccga tggatttcga ctcgctcaag aaagcgggga ctgggctcgg atcggccggc
     3901 gtcatcgtgg tggatgatgc aacgtgcatg gtggcccaga cgctgaaatt ctcaaatttc
     3961 tttaaggaag aaagctgcgg acaatgtccg ccctgccgta tgggaacgat caatctcgcg
     4021 tcgttgatga cgaagattga acagggcgaa gggacgcaaa aggacttgga cagtctgctc
     4081 caactctgtg gatttgtgaa ggggaccggc tattgcacat tggtcacagg ggcggccgtg
     4141 ctggtccaga gcagcattaa attgttccgg cacgagttcg aagaacacat ccgtttgcag
     4201 cggtgtccgt atcaagcagt cgccgtcggc gtgagcacac attgaggaaa cctgatgccg
     4261 cacgtgacat ttctgcatcc ggaagggaag agcggggtcg taaccagaaa tcttacgttg
     4321 ctcgatgccg ccaaggagtt ggggtttccg ctcaaccacg actgcggggg aaatgcttca
     4381 tgcacgacct gccgagtcga agtccaggct ggcggcgagc atttatccga aatcgagttc
     4441 gatgagcagg acctgttgga tcgcgaagcg ctcagagaac catggcatcg gttggggtgc
     4501 caagcgaaag tactgggaga tgtggtcgtc cgggtgccgg aaaccaagtg ggcggcgcca
     4561 gccacgggaa attcagaagc ccagggtgtt gatattcggt aaagaggtgt tacactaagc
     4621 caatccaggt agaccaaaga ggctggcctg gttatagagg aggaacacga tggtcacgat
     4681 tacgacggtg gcagagcaga aaatcaagga attgatgacg gaagaaaaag acgtcgtcgg
     4741 gctgcggatc tatgtccgtg gcggcggctg tcacggctat cagtacggaa tggcgttcga
     4801 atccaaaatg tccgatgacg acaccgtgat cgaaaagggc gacgtgaagg tcattatgga
     4861 ttcgcagagt gcgccgctct tgcagggcgc ggaagtcgat tacgtggata gccttcaagg
     4921 atccgggttc tccattaaaa atcctcaagc caagacgacc tgcggctgcg gcagttcgtt
     4981 cagcgcctga cccggcttga tcgcgttgcg acaggcgaat cgaacgcacg gattgtaatc
     5041 aaggccagga ttgcccatac gtagtgggta atcctggctt tgtgttttca cgaaagatgc
     5101 aggcgaatac cgaggcgaaa tggctcaagc tagcgtaacc agacgatatc ggttttgcgc
     5161 cgcccatcgg ctacacaccg accacctctc tcctgaggag aactgggctg cctttgggaa
     5221 atgtaacaat cccaacggac atggccataa ttacgtggtg ctggtgacgg tcaagagcgg
     5281 gctccaatcg actggtggta aagccgccaa tcttgcagcg ctggaccggg tggtcacgga
     5341 cacgatcatc acgagattcg atcatcacga tttgaaccag gaccctgagt ttgccgaccg
     5401 gacaacgaca ggagagaacc tggtaaaact gatctgggat cttctcgccg tgcaactgcc
     5461 gatcggatcg ttggaaagag tcggcgtggt cgaaaccagg gataactatt ttgagtatgc
     5521 aggtacgacg aagcccgaag ggggatcgca tggctaaact ggcggtggtt cgccgacggc
     5581 ggcaggaggg ggaagacgtg agtcggagtg gaagatcccc tgatctgacc ctgcttcagt
     5641 ccctggtcac ccagatgttg ctcgctctcg gcgaaaagcc acgccgaaac ggattaatga
     5701 atacccccga gcgggtagcg aaagccctgg ccttcatgac tcagggctat caacgaaaca
     5761 tcgaccatct gctcaacgga gcactatttc cgatcgagta cgacgagatg gtcatcgtga
     5821 aagacatcga cttcttcagt ctctgtgaac accatttgct gcccttcttc ggcaaggtcc
     5881 acgtgggcta cctgcccaat aagaaagtgg tggggttgag taagatcccg cgcatcgtgg
     5941 atacgtttgc aaggcgactg caagtgcaag aacggctgac gacacagatt gccgaaaccc
     6001 tcgaatcaaa gctcagcgcg aacggggtgg gcgtggtcat cgaagcgcga catctctgca
     6061 tgatgatgcg gggggtagaa aaacagaaca cggtggcggt tactagctcg atgctcggta
     6121 cattccgcag ccagcaacaa actcgcctgg agtttctgaa gctcatccgc cgaggcagcg
     6181 tcggcgactc ggactaagtc ttcgcacaag tcaagacgaa cgcctcgacg atctgattaa
     6241 acgcttccgg ctgctcaaga ttcgacagat gtgccgctcc tggaatggtc gtcagatgcg
     6301 ctctagggat ctggtcggcc ataagccttg agtcattggc gggagtgacc acgtcgagtt
     6361 cgccgacgat gatctgcacg ggacaggtga tctgacgcaa gagcggaacg gaatccggcc
     6421 gctcggccat ggccatcaag tcccccgcaa tcccactaat ttggttgcct tcaatcatcg
     6481 atcgcacgtt ctggaccagt gttggattcg tctcgatggt cccggggctc agcaatttgg
     6541 gaatcatgag gtcggcaatc gcagccggtc ctttggtata ggcgatttgg gccatctgga
     6601 atcgtccggt tctaccttcc ggcgtatcgg cctgcgcccg tgtgtccgcc agtattaagc
     6661 ccttcacgcg atcggcatac tttcgataga acgcaaacag gatatagcct cccatcgaca
     6721 gtccgacgaa caccgcctgc gggactgaca gatggtccag cagggcccga acatcatcgg
     6781 ctgcctgctc cagggtgtaa tgccaaagcg gggcgtcgga ctcaccgtgc cctcgtagat
     6841 cgatcgtaat gacacgatgg cgagacgaga gagcctcttc ctgcgcagcc cacatagtcc
     6901 ggttcagcgg aaaagcatgg agaaacacga cagggagccc ggtccctgtg tcggcatacg
     6961 caagcgtaat gtcgttgatc tgagccttca tgggaacacc gaggtttcgt accatcgagc
     7021 ggttcaatcg tcaagtaagg ttgaccgatg atcggttcgc gagcatttgc ggtcggtcag
     7081 gaaaggtgta tagaattctg tcacgtagtt atggcacgcg accagcacca gccagctgat
     7141 ttgtttactg ctccgggaac aacacccgat acaagcatta gtccgctcgc cgcgcgcatg
     7201 cgccctcaat cgtttgagga ttttatcggg caagacgaga tcgtcggccc ggaccgtccg
     7261 ctccgcaaag ccatcgaagc ggatcggttg tcgtcgatca ttttctgggg accgccgggc
     7321 tgcggtaaaa cgacattagc ccatcttgtc gctcgtcata cgaaggcaca cttcgtgtcg
     7381 ttctcagccg taaccagcgg aattcctgag ctacgtgaag tcgtcagagc ggccgagcat
     7441 cgattggcaa caagtcacca aaa
//
LOCUS       DATDNW010000032         7658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_891162, whole genome shotgun sequence.
ACCESSION   DATDNW010000032 DATDNW010000000
VERSION     DATDNW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891162"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VI359_00810"
     CDS             complement(<1..485)
                     /locus_tag="VI359_00810"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="catalyzes the conversion of dihydroorotate to
                     orotate in the pyrimidine biosynthesis pathway; subclass
                     1A is a dimer formed by two identical PyrD subunits each
                     containing an FMN group; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEY6972819.1"
                     /translation="MDLSTTIAGVKFPSCFMNASGALCVTREELLALGRSRAGAIVTK
                     SMTVEAREGNPSPRYYGFPGGSINSMGLPNLGYKAYAELIPELKRCGKPVIASVAGLC
                     EEDFPTITQLINASGPDLIEVNLSCPNIPGKPQIGYDAEASERLLKRLRRIITVPMGV
                     KL"
     gene            complement(485..1183)
                     /locus_tag="VI359_00815"
     CDS             complement(485..1183)
                     /locus_tag="VI359_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6972820.1"
                     /translation="MVKLNQVSKAFERGGLTVTALDHVTVEILAGEFCAFIGPSGCGK
                     STLLNLVAGLDHPTSGAIILNGRQTDRFTRDEWTHARREWLGLVFQAFHLVPGLTAAE
                     NVAFPLLLRGERGVGVRTRVDEVLELVGMTHRRNHRPGELSGGEQQRVAVARAIVHRP
                     HIILADEPTGNLDSQHGGEIVALLRTLVAHFQLTVLLVTHSATAASAADYTWMMRDGH
                     LITKTSQRAVPVEA"
     gene            1692..2144
                     /locus_tag="VI359_00820"
     CDS             1692..2144
                     /locus_tag="VI359_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY6972821.1"
                     /translation="MMTALPDNLETDLIMPVPLHSTRLRTREFNQSLLLADHVARHLT
                     RPVSANNLIRKVATDPQTTLSRQARLRNLRKAFAIREPHDIVDRRILLIDDVFTTGTT
                     VNECAKTLKKAGAASVTVLTLARTVDAGLVPDHIFAEHAPSSLTGFGA"
     gene            2148..2492
                     /locus_tag="VI359_00825"
     CDS             2148..2492
                     /locus_tag="VI359_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6972822.1"
                     /translation="MPIYEYRCLACQKRSTILVLSLANQSTAACKHCGSMKLERLMSR
                     FAAPKSEEARLESLADPSNLSGLDENDPQSMERFMRKMGREMGEDLGDDLSEGMEPDE
                     TDLQESGDTDSL"
     gene            2604..2801
                     /locus_tag="VI359_00830"
     CDS             2604..2801
                     /locus_tag="VI359_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6972823.1"
                     /translation="MAKEPKSEIMTAEETCRYLKITPRTLYRYLRARQIPAFKLGKEW
                     RFVRSDLEQWIRDRTRSPLAS"
     gene            2818..3258
                     /gene="mraZ"
                     /locus_tag="VI359_00835"
     CDS             2818..3258
                     /gene="mraZ"
                     /locus_tag="VI359_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY6972824.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQVESEGQAVVFLKGPEESIL
                     IYSMKEWEKVLERTKATLDEDQSRLFMHFVVSEAGTSEVDKAGRILIPGRLRKLVPLD
                     EEQEIILVGLYHRMEIWNPSEWRRYIARSEDRYEQNMAKILNLL"
     gene            3269..3850
                     /locus_tag="VI359_00840"
     CDS             3269..3850
                     /locus_tag="VI359_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEY6972825.1"
                     /translation="MPTFRRPIKISPSRLPVSILSRQNPESRSAAPRAGERFARKSPR
                     PIPFTPQSAVSLTDPHSVLLTLPVPPSVNHQYATVNGRRLLSSAGRAYKAHVAQLVWL
                     TLTQSAHRSSLLPQLKSEPLALSIRFYFTSALRRDVDGGLKIAQDALCEGLGINDNRI
                     VETHLYKNVDKTNPRIEVLLSTISNTERSLVTE"
     gene            complement(3904..5139)
                     /locus_tag="VI359_00845"
     CDS             complement(3904..5139)
                     /locus_tag="VI359_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6972826.1"
                     /translation="MPAFVWLTVVTAIRILGRNRLRAGLTMLGIVIGVAAVIAMVSIG
                     EGAKRAVQAQIATMGTNVIIMWPGATTASGVRGAQGSAVTLTVSDAVELKKRIPLLVD
                     TGWAKRDVMQIVNGNRNWNGTINGVSPSYLTIRDWSFTSGGPFTQADLDSAARVALIG
                     QTVAENLFDPGEEPVGAVIRIKNVPFRIIGVLAPKGQSAQGSDQDDVIFIPFSTAERK
                     VFGTLFIGSVGSLFASTDRTDDLPEAVQQIREVIRARHRLQADQADDFTVRTQVDIGK
                     VQEGTNQTLTMMLFAIASVSLLVGGIGIMNILLVSVTERTKEIGVRMAVGAKRRHIVM
                     QFLIEAMTLSLVGGIVGILLGIVGAKLTTVVAGWPTIISVNVVMTAFFFSLIVGLFFG
                     LYPANKAARLNPIEALRYE"
     gene            complement(5140..5859)
                     /locus_tag="VI359_00850"
     CDS             complement(5140..5859)
                     /locus_tag="VI359_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6972827.1"
                     /translation="MPPLIACDDIWKIYSVGDVEVHALRGLTLMINQGEFVAIMGSSG
                     SGKSTLMNIIGCLDQPTRGHYWLNGADVSHLRPDQLADIRNRQIGFVFQSFNLIPRTS
                     ALENAQLPLFYRKLSLKDQRGQAAAALGRVGLHGREHHYPTQLSGGQQQRVAIARALV
                     TSPSILLADEPTGNLDTQSSQEIMGILDKLNREEGITIILVTHEPEIATYASREVMIK
                     DGQVLSDRMTRTRPSASAVEV"
     gene            complement(6008..7273)
                     /locus_tag="VI359_00855"
     CDS             complement(6008..7273)
                     /locus_tag="VI359_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6972828.1"
                     /translation="MRRLILIASVLTVALVIGGYVFFNGERKVPVRYRFASIERGAVV
                     SLVTATGMINPVVSVQVGTQVSGMIKSLHADFNSVVKAGDVVAVIDPEPFKARRDQAA
                     SNLEMAKANAARARTDLAQRKRELDRAKSLVAQQFVSENDVDVALTNFQSAEAQVNLV
                     VAQVKQAEAALNAAELELKYTIIRSPVNGIVVARNIEVGQTVAASFATPNLFLIALDL
                     TKMQVDTNVSESDIGGMTEGKEATFTVDAYPGQRFSGTIRQVRLAPINVQNVVTYNVV
                     VTVDNQDLRLKPGMTANVSIVVAQKEDVLKVPNAALRFVPPRTDQVGLEGKSVRTSPV
                     QRGPSPGGSLISVNRSIWRQGPDGELESVSVQTGISDGTATELVSGSLSEGDEVIVGI
                     EQFRGERKASDLPPGFGGGGQRRSRDRGM"
     gene            complement(7435..>7658)
                     /locus_tag="VI359_00860"
     CDS             complement(7435..>7658)
                     /locus_tag="VI359_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY6972829.1"
                     /translation="TPPAMSAPSGLSSSVNLNRASASDMVLALGLSKDMADRIVANRP
                     YRVKGELVAKNVVPKETFDMIKDRISVNP"
BASE COUNT         1693 a         2344 c         1947 g         1674 t
ORIGIN      
        1 agtttgacgc ccatcggcac ggtaatgatt ctccgcagac gcttgaggag ccgttcagag
       61 gcctcggcat cgtaaccgat ctggggcttg ccgggaatgt tcgggcagga caggttcact
      121 tcgatcagat cgggcccgct cgcgttgatc agctgggtga tcgtcggaaa gtcctcttcg
      181 cagagtcctg ccacactggc tattacgggt ttcccacacc gcttgagctc tggaatcagt
      241 tcggcatagg ccttgtaccc aagattggga agtcccatgg aattgatcga tccaccgggg
      301 aacccgtaat agcgcggaga aggattccct tctctcgcct cgaccgtcat cgatttcgtc
      361 acaatcgcac cagcgcgcga tcgacccaag gccaagagtt cctcccgcgt cacgcacaag
      421 gcgcccgacg cattcatgaa acagctgggg aatttcactc cggcgatcgt ggtcgacaga
      481 tccatcaggc ttccaccggc acggctcgct gcgacgtttt cgtgatgagg tgtccgtccc
      541 gcatcatcca cgtataatcg gcagccgacg cagcagtggc actgtgggtg acgagtaaca
      601 cggtcagttg aaagtgcgcc actaacgtgc gcagtaacgc aacaatttcc cctccatgct
      661 gagaatctaa attaccggtc ggctcatccg cgagaattat gtgggggcga tggacgattg
      721 ctctggcgac cgctacccgc tgttgctcgc cacccgagag ttcaccggga cgatggtttc
      781 gtcgatgggt catgcctacc agttccagca cctcatcgac ccgcgtccgc acacccacgc
      841 cacgttctcc gcgtagtagg agcgggaacg ccacattttc tgccgcggtc agcccaggca
      901 cgaggtgaaa cgcctgaaag acgaggccca gccactcacg tcgcgcgtgg gtccattcgt
      961 cgcgcgtaaa ccggtcggtc tgtcggccat tgaggataat tgccccggag gtggggtgat
     1021 ccaaccccgc gacaagattc agcaacgtgc ttttcccgca cccgctggga ccaatgaagg
     1081 cacagaattc accggcgagg atttcaacgg tcacatgatc taatgccgtc accgtcagtc
     1141 cgccacgttc gaaagctttt gacacctgat taagcttgac catattgacc cattggtcgt
     1201 tcctcgctta tcgaattatc ctcgtatatc acaaccattt acagagccac aaccttgaca
     1261 gcaccggcgt cttctcttag aagaatccct cgaacgtacc gtctgctcag aagttcttca
     1321 tcatggacta tgttggtatg gcagaccgaa ttgtcgagct ctctctccag gccttacggt
     1381 ttatattccc ggtagactgc acggcctgcg ggcgtaccct cagcaccgac cctgtccctt
     1441 ttttctgtcg agattgttgg caccgcattg tcccgtttcg ccgaccctct tgtgtgggtt
     1501 gtggtcaacc tttcgtctcc gaagcggcaa cctcttacac gccggatcgc cactgccaag
     1561 attgtgagga acgccctccg gcattcgaac gagcctggac gctctttccc tatatccctc
     1621 cgctacaaga tgctatccgt ttatttaagt atcgaggtaa aatcgctctg gcacggccat
     1681 tagctgctct catgatgacc gccctgccgg ataatcttga aacggacctt attatgccgg
     1741 tgcccttgca ctcaacccga ctccgcactc gcgaattcaa tcaatcactt ctgctcgccg
     1801 accacgtcgc gcggcacctg acacgtccgg tctctgcgaa caatctcata cgcaaggtcg
     1861 cgacagatcc tcaaacgaca ctgtctcgtc aggcccgcct gcgaaatctg cgcaaggcat
     1921 ttgccatccg cgagcctcat gacattgtcg atcggcgcat tctcctgatt gacgacgtct
     1981 ttacaacggg aacgaccgta aatgaatgtg ccaagacact caagaaagca ggcgccgcct
     2041 cggtgactgt cctgacactg gcccggaccg tcgatgccgg cctggttccc gatcatattt
     2101 ttgcggaaca tgcaccgtct tcgctgacgg gtttcggagc gtgaccgatg cccatttacg
     2161 aatatcgctg cctcgcctgc cagaaacgga gcactatcct cgtcctgagt ctcgcaaatc
     2221 aatcaacggc agcgtgcaaa cattgcggca gtatgaagct tgaacgtctc atgtcccgat
     2281 ttgcggcgcc aaaatctgaa gaagcacgtc tcgaatcgct ggcggacccg agcaatctca
     2341 gtggactcga cgaaaacgac ccgcaaagca tggaacggtt catgaggaag atgggacgtg
     2401 aaatgggtga ggacctcggc gatgatttat ccgaaggcat ggagcctgat gagactgatc
     2461 tccaggaatc aggtgacact gacagtctgt gacaggaatt gtcatctatt ttcacttgac
     2521 agatcagctg tttttcccta ccctcgcctc taagcgcggg agtcttgtga ggcccatgag
     2581 cgatttacaa ttgcggttct accatggcca aagagcccaa gagcgaaatc atgacagcgg
     2641 aagagacctg ccgctatttg aagatcaccc cgcggacgct ctaccgctac ctgcgcgctc
     2701 gtcagattcc cgccttcaag ctcggcaaag aatggcgatt tgtgcgctcg gatctggaac
     2761 agtggattcg cgaccgcacc aggagcccgc tcgcttcgtg aaggagacca cacactgatg
     2821 ttcgccggcg aatatctctg caaagtcgac gagaagggac ggttcatcgt cccctcgccc
     2881 attcgagagc aggtcgaatc agaggggcag gcggtcgtct ttctcaaagg gccagaagaa
     2941 tcgattctga tttattcgat gaaggagtgg gaaaaagtcc tcgaacgaac gaaagccaca
     3001 ctggatgagg atcagagtcg actcttcatg cactttgtcg tatcagaagc cggcacctca
     3061 gaagtcgaca aggcgggccg cattctgatt cccggacggc tcagaaaact cgtgccgctg
     3121 gatgaggagc aggagattat cctcgtcggc ctgtaccatc gcatggaaat ctggaatccg
     3181 agcgagtggc gtcgctatat cgcccgctcc gaagatcggt atgagcagaa tatggccaag
     3241 attctcaatc tcctctagcc tgtcgcgcat gcccaccttt cggcgaccga tcaaaatttc
     3301 cccatccaga cttccggttt ccatcctctc ccgtcagaat ccagaatcga gatccgcggc
     3361 gccgcgcgcg ggtgagaggt ttgcgcggaa gtcccctaga ccaattccct ttacccccca
     3421 gagtgcagtc tcgctgaccg atccccattc cgtattgctg acgttaccgg tgcctcccag
     3481 cgtgaaccat caatatgcca ccgtaaacgg tcgtcggctt ctctcctccg caggtcgtgc
     3541 ctacaaagcc cacgtggcgc aattggtctg gctgaccttg acgcaatcag cacaccgatc
     3601 aagtttgctg ccgcaactca agtcggaacc gctggcgctc tcgattcgat tttatttcac
     3661 ctcggctcta cggcgagacg tcgatggcgg gcttaaaatc gctcaggatg cgctctgcga
     3721 aggactgggg attaatgaca atcgcatcgt cgaaacccat ctctataaaa atgtcgataa
     3781 gaccaatccg cgcatcgaag tcctgctctc gacgatatcc aacacagaac gcagtctcgt
     3841 gaccgagtag aacgatccct atcgtcctgt cacgatgcgg ctggtctggc agccggaaaa
     3901 gttttattcg tagcgcaagg cttctatggg attcaggcga gcagctttat tcgcagggta
     3961 gaggccgaag aagagtccca cgatgagaga gaagaaaaat gccgtcatca ccacattgac
     4021 ggaaataatc gtcggccatc ccgccaccac ggtcgtcagc ttggcaccga cgattcccaa
     4081 tagaatgcca acgatccccc cgaccaggct cagtgtcatg gcttcaatca gaaattgcat
     4141 cacgatgtgc ctgcgtttcg cgcccaccgc catccggacg ccgatctctt tggttcgctc
     4201 tgtcaccgac accagcagga tattcatgat gccgatcccg ccaaccagca aggagactga
     4261 ggcgattgca aacaacatca tcgtgagcgt ctgattcgtc ccttcctgca cttttccaat
     4321 atcgacttgc gtccggaccg taaagtcgtc ggcttgatcc gcctggagcc gatggcgggc
     4381 tcgaattacc tcacgaatct gctgaacggc ttccggcagg tcatcggttc ggtctgtcga
     4441 tgcaaagagc gaccctacgg aaccgatgaa caacgtccca aatacctttc gctccgcagt
     4501 ggagaagggg ataaaaatta cgtcgtcttg atcggatcct tgcgcggact gccccttggg
     4561 tgcaagtact ccaataatac ggaacggcac gtttttgatc cgaatgactg caccgactgg
     4621 ctcctcacca ggatcgaaaa gattctctgc cacggtctgg ccgatgagcg cgacacgtgc
     4681 cgcgctgtcc aagtctgctt gggtgaaggg accgccactg gtgaacgacc aatcgcggat
     4741 ggtcaggtag ctcggtgaaa ccccgttgat cgttccgttc caattccggt tcccattgac
     4801 gatttgcatc acatcacgct tggcccaacc cgtatcgacc agcaatggaa tacgtttctt
     4861 caattcgacg gcatcggata ccgtcagggt caccgcactc ccctgcgccc ctcgaactcc
     4921 actcgccgtc gtggcaccgg gccacatgat aatgacgttg gttcccatcg tcgcaatctg
     4981 tgcctggacc gcacgctttg ccccttcgcc gatgctcacc atcgcgatca cggcagcaac
     5041 tccgatgacg ataccgagca tcgtgaggcc ggcacgcagg cggttgcgcc cgaggatccg
     5101 gattgcggtc accaccgtga gccacacaaa cgctggcatc tagacctcaa cggcacttgc
     5161 agatggacgt gtccttgtca tccggtcgct taacacttga ccatccttga tcataacctc
     5221 gcgcgatgcg taggtggcga tttccggctc atgcgtgacc aggatgatcg tgataccttc
     5281 ttcacgattc aacttgtcca gaattcccat aatctcctga cttgactgcg tatccaggtt
     5341 tcccgtcggc tcgtcagcaa ggagaatgga aggtgaggtc accaacgctc gcgcgatcgc
     5401 gacccgttgt tgctgtccgc cagacaactg cgtgggataa tggtgttctc tcccgtgcag
     5461 tccgacccgt cccagcgccg cagcggcctg tcctcgctgg tccttgagcg acagcttgcg
     5521 atagaacaat ggaagctggg cattctccag cgcactcgtc cggggaatca gattgaagct
     5581 ctgaaaaaca aatccgatct gccgattccg aatgtcggct aactgatcgg gccgtaaatg
     5641 gctcacgtcg gctccattca accaataatg ccctctcgtc ggttgatcga gacagccgat
     5701 gatattcatg agcgtcgatt tgccggaccc ggatgacccc atgatcgcga caaactcccc
     5761 ttggttgatc atgagagtca gaccgcgcag cgcgtgaact tcgacgtctc cgacagaata
     5821 aatcttccag atgtcgtcgc aggcgatcag aggaggcatc gttgatccat cgtcagaact
     5881 ctctccacta ttactccata tgcgatggcc agtcgggata tggacctcat cttccaccta
     5941 gcgatgctca atcccgctgt aagagacggg aagaacagaa cgcagggatc ccgttcccgt
     6001 gtttccatta catgcctcga tcacgggacc gccgctgccc cccacctccg aaccctggag
     6061 gcaaatcact tgctttccgc tcccctcgaa actgctcgat cccgacgatg acctcatctc
     6121 cttcagacaa ggagcccgag acaagttccg ttgcagttcc atcggaaatc ccggtctgca
     6181 ccgaaacgga ttccagttct ccgtctggtc cttgtctcca gatcgaccga ttcaccgaga
     6241 taagactccc acccggcgac ggaccacgtt gaaccggcga ggtccttacc gacttgcctt
     6301 caagaccgac ctggtcggtc cgggggggta cgaaccgcaa tgccgcattc ggtaccttga
     6361 gcacatcttc cttctgagca accacgattg aaacgttcgc cgtcattccc ggcttcagtc
     6421 tcagatcctg attatcgacc gtgaccacga cattgtacgt cacaacattt tgcacgttaa
     6481 tgggagccag ccgcacttga cggatcgtcc cggaaaatcg ctgacctgga taggcgtcaa
     6541 ctgtaaaggt agcctctttg ccctccgtca tcccgccgat atccgactca ctgacattcg
     6601 tatccacctg catcttcgtg agatccaacg caatcaggaa gaggttcggc gtcgcaaaac
     6661 ttgccgccac ggtctggccg acctcgatat ttctggcgac cacgatgccg ttgaccggcg
     6721 accggataat cgtatatttg agctccaact ccgccgcatt gagcgccgct tcggcctgct
     6781 tcacttgcgc cacaacaaga ttgacctgtg cctcagcgct ctgaaaattt gtcaacgcga
     6841 catccacgtc gttttccgag acgaactgct gagccacgag tgacttcgcc cggtccagtt
     6901 cgcgtttgcg ctgcgcgagg tccgtccttg ccctcgccgc gttcgctttg gccatttcca
     6961 gattactggc ggcctgatcc cggcgagcct tgaacggctc cgggtcgatc acagccacga
     7021 cgtcgcctgc cttcaccacg gaattgaaat cggcgtgcag actcttgatc attccggaca
     7081 cctgtgttcc gacctgaacc gaaacgacgg gattaatcat accggtcgcc gtcactaatg
     7141 acacaaccgc gcctcgttcg atcgacgcga atcgataccg gacagggact tttcgctccc
     7201 cgttaaagaa aacatagcct ccgatgacca acgcgaccgt caggacgctg gcgatgagaa
     7261 tcagccgacg catatctcca gcctcgcaag agcaatacaa accgagtgca ttctaccaag
     7321 aaggacagag ggaatcattc aacacaaatc gtcactgggc ttgccataaa aacagacagg
     7381 ccgtccgcca ctcggcggac ggcctgtctc actcaccggt tcatcaattt ctcgttatgg
     7441 attgacgctg atccgatcct tgatcatgtc gaaggtctct tttggcacaa cgtttttggc
     7501 cactaattct cccttgacac gatacggacg gttggcaacg attcggtcag ccatgtcttt
     7561 tgatagcccg agagccagga ccatatcgct tgcggaagct ctattgaggt tcacagagga
     7621 cgacaggccg ctcggagcac tcatcgcagg gggcgtac
//
LOCUS       DATDNW010000033         9323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_415161, whole genome shotgun sequence.
ACCESSION   DATDNW010000033 DATDNW010000000
VERSION     DATDNW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415161"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VI359_00865"
     CDS             complement(<1..491)
                     /locus_tag="VI359_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY6972830.1"
                     /translation="MDRPLLLSLAACTDPALVGGKTAGLARLIAHGFRVPPGLCVTTE
                     AYQSSLQAQGFSLAERWNHLLRTSTEDRPRVLVDYQAVIRNVECQDLMAQVHQAVSRF
                     DQPAHTLWAVRSSATNEDAIHASFAGLYRTHLGVPLSGMEWAIKDLWASVWDERVIQY
                     HQQS"
     gene            complement(506..1843)
                     /locus_tag="VI359_00870"
     CDS             complement(506..1843)
                     /locus_tag="VI359_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY6972831.1"
                     /translation="MKIRLPSSPARSLLTKLIGLGLEAVQAASAVRRAVIRRGDELII
                     APRRYDLRRYDRVVAVGAGKATAPMARALERCLGSRLHSGLVVVKYGHAVPTAKIVVA
                     EAGHPIPDRAGQRAAARLQSMVSALTSRDLLIVLLSGGASSLLPAPVRGITLVDKQAT
                     TDRLLRCGATIQEINTVRKHLSTIKGGRLAESTPATVVTLILSDVLGDDLSAIASGPT
                     APDPTTYRDAVAILRRYRIWRTVPPRVRQHLLQGQGGCLAETPKPRSSIFRQVHHHVI
                     GSNMIALTAVTRAARDAGLRALVHSATLKGEARVAGERFGSIARSIVNKRHPVPPPCC
                     ILAGGETTVTVRGKGKGGRAQEFAVAAARKIAGLQRVWVAAIGTDGTDGPTNVAGAVV
                     DGETTARAKRLGINLNHALQRNNTYPALKRLRCHITTGPTGTNVNDLYLLLVL"
     gene            complement(1855..2919)
                     /locus_tag="VI359_00875"
     CDS             complement(1855..2919)
                     /locus_tag="VI359_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY6972832.1"
                     /translation="MTAWVWRMVMLGTIMGVCVLPGPTGAQPVSESPNNSTQLATNGE
                     PDRELMLPEPLEGPNEPEDRLVILPEIKREGERFFLSSFKLPDKITFAGVPVPLDNWQ
                     VRERIEYEFYQFLEDQGESIILAKRTGRCFPPAEKQLAEAGLPDDLKYMLLVESKCVA
                     AAYSRAKASGPWQFIGSTGKRYKLKSDSYRDERRNLEMSTEAAVKYLRYLKDFKENDW
                     FLAMASYNAGEERIRKLLKEQKITDYWKMHGPRETMRYVPRIIAAKEIYSQPEKYLGL
                     SKKDLYMPLETETITLNVKEPQRTLTSIAEEFGTYFLELKMLNPELKKDFLPRGTYQI
                     KVPRQSCPSRCFKQDKVQTP"
     gene            3038..3742
                     /locus_tag="VI359_00880"
     CDS             3038..3742
                     /locus_tag="VI359_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6972833.1"
                     /translation="MRNLAILNELISNCRICPRLVTYRAAVAKAKRKQFLNWTYWGRP
                     VPGFGDPRARLYVLGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQAESQHRD
                     DGLMLTDCYIGATVRCAPPDNKPAPEEFEACRPYVRHEMRLLSRMEAVVVLGKIAFDH
                     YFKACRDEGFEVPSPLPAFSHGASYSLPWNITLICSYHPSQQNTFTGKLTRPMFHQVF
                     ATARRHLGRRHTGTRR"
     gene            complement(3745..4392)
                     /gene="fsa"
                     /locus_tag="VI359_00885"
     CDS             complement(3745..4392)
                     /gene="fsa"
                     /locus_tag="VI359_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY6972834.1"
                     /translation="MKFYLDTASVKEIHEAASLGLLDGVTTNPSLVAKEGRVFREVLV
                     EICNIVDGPISAEVVSLEADAMVKEGKELAKIHKNIVVKVPLIPEGLKATKRLAAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQIVTIYKNYDYKTYV
                     LVASVRHPQHVVEAALAGGHICTMPFAIFQQMVKHPLTDLGLKKFLADWDAQSRK"
     gene            4604..4918
                     /locus_tag="VI359_00890"
     CDS             4604..4918
                     /locus_tag="VI359_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972835.1"
                     /translation="MMPRLLWILLSCVFLTACGGSNPYLEASLKPAELQGKDKAWFEK
                     NWGAPDGKAPRFFGGETWTYYRIAGGKSGLPMFNFSPNQCQITLKFDKEEKLSDYSYS
                     GC"
     gene            complement(4903..5166)
                     /locus_tag="VI359_00895"
     CDS             complement(4903..5166)
                     /locus_tag="VI359_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY6972836.1"
                     /translation="MYRIILILALLVVLYFLLRSAIRELKHRGAGDRLPLDKNQMVQD
                     PVCRVFVPRGSATTEDIGGQTYYFCSRACANVFQKQLAGQQPE"
     gene            complement(5760..6563)
                     /gene="dapB"
                     /locus_tag="VI359_00900"
     CDS             complement(5760..6563)
                     /gene="dapB"
                     /locus_tag="VI359_00900"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY6972837.1"
                     /translation="MIKVIVAGAAGRMGCRLVALVKDSTALTLMGAIEHKGHHALGDD
                     AGETAGSGRAGVAITDDLSALLDRGEVVIDFSAPEATLNHLRVVAQHRRAMVIGTTGF
                     SAAEMEEVKTLARQVPCVCSPNMSVGVNLMYKVIGEMAKTLGDEYDIEVIEAHHRLKK
                     DAPSGTALRIAEVLARAVNRDLNQVGVYARKGLIGERKKGEIGIQTIRAGDIVGDHTI
                     LFGGMGERIEVTHRATSRDTFARGALRAARWVVRQPPGFYDMMDVLGLR"
     gene            complement(6592..7464)
                     /gene="dapA"
                     /locus_tag="VI359_00905"
     CDS             complement(6592..7464)
                     /gene="dapA"
                     /locus_tag="VI359_00905"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY6972838.1"
                     /translation="MFTGSLVAIVTPFRKGKIDEQAFGSLIDWQIANGTNGIVPCGTT
                     GESATLSHEEHNRVVELTVEIVNRRVPVIAGTGSNSTDEAIALTRHAKRARADGALLI
                     TPYYNKPTQEGLYRHYKAVAEAVDIPLVLYNIPGRTGVNMLPATIARLSAIKNVVGVK
                     EGSGSVQQASDIVQMCGERLTVLAGDDALTLPMMAVGGKGVITVTANIVPADMANLVK
                     AFAEGNIEGARRIHFKLSPLFAALFYETNPIPVKEALGMMGKIDPELRLPLCAMATDT
                     REKLARVLKDMGLI"
     gene            complement(7511..8773)
                     /gene="lysA"
                     /locus_tag="VI359_00910"
     CDS             complement(7511..8773)
                     /gene="lysA"
                     /locus_tag="VI359_00910"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY6972839.1"
                     /translation="MHSFEYRQGELYCEQVPVSRIVKELGTPCYIYSHATLVRHFQVY
                     DRAFAAIPHIIAFAMKANSNLAILRLMAKEGSGVDIVSGGELFRALKAGVPPSKIVFA
                     GVGKSAEEIRDALKADILMFNVESSAELQVLNEVAASVGKKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALDEFKLASSLGHIDVVGVHKHIGSQLTEVTPFVEALKKIL
                     KLVEVLRSTGINIRYINIGGGLGITYSDETPPLPQDLADAISPMVRDLNVTLIMEPGR
                     VIVGNAGILVTKVLYMKEGEAKKFVIVDAGMNDLIRPSLYGAYHEIRPLSEAVLQRGK
                     HQVDVVGPVCESGDFLAKDRSLPDVRPGEMLAVMSAGAYGFVMSSNYNSRPRVPEALV
                     KDGEIHVIRARESYSDLVKGEQIPAFLG"
     gene            complement(8766..>9323)
                     /locus_tag="VI359_00915"
     CDS             complement(8766..>9323)
                     /locus_tag="VI359_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972840.1"
                     /translation="LSGTSQRLVARWEMNREFRKLVKRSCPKGSLSPLASVLKNLQAK
                     LPVLDTDQLIPSALHALKQRFDPKTIVPENLALNIKTKRHDKFAALFVDLEGTLDRLN
                     AQELLARIKQAAEQAKMDVIVNFEHLKLATPDALKALIDSEAIKAAIPHVRLRYRKFK
                     DAFEASLQGLSLSGLEFLSEDLQDA"
BASE COUNT         1985 a         2760 c         2528 g         2050 t
ORIGIN      
        1 gattgctgat gatactgaat cacccgctcg tcccaaaccg atgcccagag atccttgatt
       61 gcccattcca tcccggacag cggcacaccg agatgcgtcc gatacaaccc cgcaaagctg
      121 gcatggatcg catcttcatt cgtcgcggat gatcggactg cccagagggt atgagccggc
      181 tggtcgaatc ggctgacagc ctggtgcacc tgcgccatca gatcctgaca ttccacgttt
      241 cgaatcacgg cctgatagtc aacaagaacg cgtggcctgt cttctgtcga tgttcgtaga
      301 agatggttcc accgctccgc cagagaaaag ccttgagcct gcagagagga ttgataggct
      361 tcggttgtca cacacagacc cggcggaacg cgaaagccat gagcgatgag gcgagctaac
      421 ccggccgtct tcccaccaac aagagctgga tccgtgcaag cggccaaaga aaggaggaga
      481 gggcgatcca ttgaagcgat actacctaga ggacaagaag caggtaaagg tcattgacgt
      541 tcgtcccggt gggaccagtc gtgatgtggc aacggagtcg tttcagggca ggataggtat
      601 tattgcgttg taatgcatga ttgagattga tccctaggcg cttcgcccgg gctgtcgtct
      661 cgccatcgac aacggcaccg gcgacgttcg tcgggccatc cgtcccatcg gtaccgattg
      721 cggcaaccca gacccgctgg aggccggcga tcttgcgcgc tgcagccaca gcgaactcct
      781 gagcacggcc gccctttccc ttccccctga cagtcaccgt tgtttctcct ccggccagaa
      841 tacagcaagg cggaggcacc gggtgacgct tgttcacgat gctccgtgcg atcgaaccaa
      901 accgctctcc tgccacacga gcttctccct tcagtgtcgc cgaatgcacc agggccctga
      961 gtccggcgtc tctcgcggct cgggtcacgg cggtcagcgc aatcatatta ctgccgatca
     1021 cgtgatgatg gacctgccga aagatcgacg atctgggttt cggggtctca gccaaacatc
     1081 cgccctgtcc ctggagcaag tgctggcgga ctcgcggtgg gaccgttcgc cagatgcgat
     1141 atcgccgcag gatcgccacc gcgtcacggt acgtcgtggg atcaggagct gtcggcccgg
     1201 aggcgatcgc gctcaggtcg tcaccgagca cgtcggaaag aatcagggtc accaccgtcg
     1261 cgggcgttga ttcagccaga cggccgcctt ttatcgtgga gagatgcttg cgcaccgtat
     1321 tgatctcttg aatcgtggct ccgcaccgca acaggcgatc ggtcgtggct tgcttatcca
     1381 ctaatgtgat ccctctcacc ggagccggca gcaaacttga cgcccctccg gaaaggagca
     1441 cgatcaggag atcgcgactg gtcaacgccg agaccatgga ttggaggcga gcggccgctc
     1501 gctgcccggc tcgatcggga atgggatggc cggcctctgc gacgacgatc ttggccgttg
     1561 gcacagcatg accgtacttc acgacgacga ggccgctatg caggcgggag ccgagacagc
     1621 gctcaagtgc ccgagccatg ggtgccgtgg cctttccggc cccgactgca acgactcgat
     1681 cgtaccggcg cagatcgtac cgacgcgggg cgatgatcag ctcatcgccc cgtctgatca
     1741 cggcgcggcg cacggcgctg gcggcctgca ccgcctcaag tccgagcccg atcagcttcg
     1801 tgagcaacga ccgggccgga gacgaaggaa gacggatctt catcagagat gaagtcaggg
     1861 ggtctgcact ttgtcttgct tgaagcagcg ggacgggcag ctctgccgcg gcactttgat
     1921 ctgataggtg ccgcgcggga ggaaatcttt cttcaactcg ggattcaaca tttttaattc
     1981 caagaaatag gtcccgaatt cttccgcaat cgacgtgagt gtgcgttgcg gctctttgac
     2041 gttcagcgtg atggtctcag tctccagcgg catatagaga tccttcttgc tgaggcccaa
     2101 gtacttctcc ggttgcgagt agatctcctt ggccgcaatg attctcggta cataccgcat
     2161 ggtttcccgt ggaccgtgca ttttccaata atcggtaatc ttctgctctt tgagcaactt
     2221 gcgaatgcgt tcttcgcccg cgttgtacga ggccatcgcc aagaaccaat cgttctcctt
     2281 gaaatctttg aggtagcgca agtacttgac cgccgcctcc gtcgacattt ccaggttgcg
     2341 gcgctcgtca cgatagctgt cggatttcag cttatagcgt ttgcccgtgg acccaatgaa
     2401 ctgccaggga cccgatgctt tggctcgcga gtaggcagca gcgacacact tactttcgac
     2461 cagcaacatg tatttgagat catccggcag tccggcctcc gcaagctgct tttcggcggg
     2521 agggaagcat cgtccggtcc gcttggcgag tatgatgctt tcgccttgat cttccaagaa
     2581 ctgataaaac tcatactcga tgcgttcgcg cacctgccag ttatccagcg gcactgggac
     2641 accggcaaac gtgatcttgt ccggcagctt aaacgagctg agaaaaaacc gttccccttc
     2701 tcgcttgatt tcgggcagga tgaccagccg gtcttccggc tcgttgggtc cctcgagcgg
     2761 ctcggggagc atcagttcgc gatccggctc cccgttcgtg gccagttgag tagagttgtt
     2821 tggcgattct gataccggtt gggcgccggt tggaccgggc aggacacaga cccccatgat
     2881 ggtgccgagc atgaccatgc gccacaccca cgccgtcata tcagtccgcc tcccatgcct
     2941 ctggacgttc atacataccc ggaggttcat gctatcagca cgtcaaaaag ccggcaagca
     3001 tgatcgactt tcagattcat gctagactgc gccgcccatg cgtaacctgg ccatactgaa
     3061 cgagcttatc tcgaactgcc gaatctgtcc gaggctcgtc acctatcgag cggcagtcgc
     3121 caaggcgaaa cggaaacaat ttcttaactg gacctattgg ggacgtccag tgcccggatt
     3181 tggagatcct cgtgcgcgcc tctatgtcct cgggcttgct ccagccgccc acggcggtaa
     3241 cagaaccggc cgcgtattta cgggagaccg aagcggcgat tggctctatg aagcgctgca
     3301 tcggttcggt ttcgccaacc aggctgaatc tcagcatcgc gacgacggat tgatgctcac
     3361 ggattgttat atcggggcca cggttcgctg tgcaccgccg gacaacaaac ccgctccgga
     3421 ggagttcgag gcttgccgtc cctatgtccg ccatgaaatg cggctcttgt cgcggatgga
     3481 agcggtcgtc gtgcttggaa agatcgcctt tgatcactac ttcaaggcgt gccgtgatga
     3541 agggttcgag gttccttctc ctctgcccgc gttttcacac ggcgcctcgt actcgcttcc
     3601 ttggaatatc acgctgatct gctcgtacca tcccagccag caaaacactt ttaccggcaa
     3661 actgactcgg ccgatgtttc atcaagtctt cgccacagca cgacggcatc ttggcagaag
     3721 gcacaccggc actcgtcgct agaattattt gcgactctgg gcatcccaat cggccaagaa
     3781 tttcttcagg ccgagatcgg tcagcgggtg tttgaccatc tgctggaaaa tcgcgaaggg
     3841 catcgtgcag atgtggccgc cagccagagc tgcctcgacc acgtgctgcg ggtgtcgaac
     3901 actggcaacc agcacatagg tcttatagtc gtagttcttg taaattgtga caatctgacg
     3961 gatcaattcc attccattcg aactgatgtc gtccaaccgg ccgatgaaag gagagacaca
     4021 ccaggccccg gccttggcag ccaggagcgc ctgtgtcggt gagaagcaga gcgtcacgtt
     4081 cactctgatc ccttccgcag ccagccgttt tgtcgccttg agcccttcag ggatcagcgg
     4141 gaccttgacc acgatattct tatgaatttt ggcgagttcc ttcccttcct tgaccatcgc
     4201 atcggcttcg aggctgacga cctccgcact gatcggccca tcgacaatgt tgcagatctc
     4261 gacgagcact tcgcggaaca cccgaccctc tttcgcaacg agggaaggat tcgtcgtgac
     4321 cccgtccaag agtccaaggc tcgccgcttc gtgaatttct ttgacgctgg cggtatcgag
     4381 atagaatttc atggctatat cctttcttga agtgacggca ttccctgatg agatcgttca
     4441 aacctattct aggaagaatc tggtggtgat gcaacgtctc tcttacctgt ggacttggtt
     4501 tgacaggtat ccggacgggg attaaaatcg cgttgtcaac gaaagataca tcacgacgtt
     4561 acacatgata cctcgcgcga taaataagat gaggagacat ctgatgatgc cccgattgtt
     4621 gtggatcctt ctcagctgtg tattcctgac cgcctgcggc ggaagcaacc cctatcttga
     4681 ggcatcgctc aaaccagcag aacttcaagg caaagacaag gcatggtttg aaaagaactg
     4741 gggcgcacca gatggcaaag caccaagatt tttcggcgga gaaacctgga cctactatcg
     4801 catcgccggt ggcaaatccg gccttcccat gttcaacttc tcgcccaatc aatgtcagat
     4861 cacgttgaag ttcgataaag aagagaagct ctcggactat agttactccg gctgttgacc
     4921 cgccaattgc ttctggaaca cattcgcgca agcccggctg cagaaataat aggtttgtcc
     4981 tccgatgtct tctgtcgttg ccgaccctcg cggtacaaag acgcgacaga ctggatcctg
     5041 caccatttga ttcttatcca agggaaggcg gtctccggcc ccgcggtgct tgagttctcg
     5101 aattgcgctg cgcagcagga agtagaggac caccagtagc gcaagaatga gtatgattct
     5161 atacatagta gggggagcga ttgagagcca caatcctatg ggacaaggtt cgtcctgtca
     5221 aggatgccga cctcggccga agactcgcga tagcattgtt ttctcattaa tgtccggcca
     5281 attggcctag cctgatctgg tcgcaaaggc ccaccttaac ccagtacttc tgatggatat
     5341 gcttgcattt gaagcgatga tacagtgcgc ccatgaagac ttgcgataaa tgccgcggaa
     5401 caatgttgcc ggaacgggca gtcgacttgg atgccggact cgcgatcatg gtgtttgcgt
     5461 gtttaaattg tggtcgtcga aaggctgcgg accaagaacc tcgcccgctc accgcccgac
     5521 attgatcccg cgggatgact caacgccagc tcctcaccta cgataagcgc tagcttttga
     5581 taccgccttt ctgagtatcc acccgaccgc gaacaggcag aatctgtaca attcacctgg
     5641 acaggcctcg cccttggctc tagtcgtctg gacaacaccc gagacgcgcc tcggatcatg
     5701 ctgaggcaga catcattttg atctggattg aagacaaatg gaattgcagc tgatgcgaac
     5761 taccgaaggc ccagcacgtc catcatgtcg tagaaaccag gcggctgtcg aacaacccag
     5821 cgcgcagctc gcaaggcacc tcgcgcaaag gtgtctcggc tagtggcacg atgcgtcact
     5881 tcaatacgtt cgcccatgcc accgaagaga atggtgtgat caccgacaat gtccccggca
     5941 cgaatcgttt gaatgccgat ctcgcccttc ttccgctcgc cgattaagcc cttccgggca
     6001 tagaccccga cctgattcaa atcacgattc acggctctcg caagaacctc ggcgatcctg
     6061 agggctgtcc cgctcggcgc atcctttttc aatcgatgat gggcttcaat cacctcgata
     6121 tcgtattcgt ctccgagcgt cttggccatc tcgccgatca ccttgtacat caaattcact
     6181 cccacgctca tattgggaga acagacgcag ggaacctggc gagcgagtgt tttgacttct
     6241 tccatctccg ccgccgaaaa ccctgtcgtg ccgatgacca tggcccgccg gtgctgcgcc
     6301 acgacacgaa gatggttgag cgtggcctca ggagctgaga aatcgatgac gacctcccca
     6361 cggtccaaca aggcggaaag gtcgtcggta atcgcgactc ctgcgcgccc gctgccggcg
     6421 gtttcaccgg catcgtctcc caaggcgtga tgtcccttat gttcgattgc gcccataagg
     6481 gttaaggcgg tcgaatcctt caccaacgcc accaatcgac agcccattcg accggcggct
     6541 cctgcaacaa tgaccttgat catagggaga cgtttcgcac gcaaccagcg tctagatgag
     6601 gcccatatcc ttaaggacgc gagcgagttt ctcgcgagta tctgtcgcca ttgcacagag
     6661 cggcaaccgc aattcgggat cgatctttcc catcatcccg agcgcttctt ttacagggat
     6721 cggattggtc tcgtaaaaca gggctgcaaa cagcggggat agtttaaaat gaatcctccg
     6781 ggctccctca atgtttccct cggcgaacgc cttgaccaaa ttagccatat ctgcaggaac
     6841 gatattcgcg gtgacggtga tcaccccctt gcctccaacc gccatcatgg gaagcgtcaa
     6901 ggcatcgtct cctgccagga ctgtgaggcg ttccccgcac atctgcacga tgtccgacgc
     6961 ctgttggacc gatccactcc cttccttgac gcccacgacg ttcttgatcg cggacagtct
     7021 ggcgatcgtg gccggcagca tgttgacgcc ggtacgtcct ggaatgttgt acagtaccag
     7081 cggaatatcc accgcctctg caaccgcttt ataatgccgg tagagccctt cctgtgtcgg
     7141 cttgttgtag tacggtgtga tgaggagggc tccgtcagcc ctggcccgtt ttgcatgtct
     7201 ggtgagtgca atcgcttcgt cggtgctgtt cgatcccgtc ccggcaatga ccggtacgcg
     7261 ccggttcacg atctccacgg tcaactcaac gacgcggttg tgttcctcat gcgaaagggt
     7321 tgcagactcg cccgtggtac cgcagggaac gatgccgttg gttccgttgg caatctgcca
     7381 atcgatcaac gacccgaacg cctgctcatc gatttttcct tttcgaaagg gggtgacgat
     7441 ggccacgagc gaaccggtaa acatgatcac tccttgcggg agcggataac tgaacctccg
     7501 cccacagccg ttatcctaaa aaggcgggaa tctgctcacc ctttaccaaa tcgctatagc
     7561 tttcacgtgc tcggatcacg tggatctctc cgtccttgac caaggcctcg ggcacgcgag
     7621 gacgggaatt gtaattggaa gacatcacga acccatacgc accggcactc atgacggcca
     7681 acatctcgcc cggacggaca tccggcaacg accgatcttt cgcgagaaaa tcccccgatt
     7741 cgcagacagg gcctacgaca tcgacttgat gcttgccgcg ctgcaacacc gcttcggaca
     7801 ggggacgaat ctcgtgatag gcgccataga ggctcgggcg gatcagatcg ttcatccccg
     7861 catcgacgat gacgaatttc ttcgcttccc cctccttcat gtacagcacc ttggttacaa
     7921 gaatcccggc attgccaacg atgactcggc ccggttccat aatcagcgta acgttgagat
     7981 cgcgtaccat cggcgagatc gcatcggcaa ggtcctgagg gaggggaggc gtctcgtcag
     8041 aataggtaat cccgaggccg ccgccgatgt tgatataccg gatgttgatc ccggtgcttc
     8101 tcagcacttc gacgagtttc aggatcttct tgagggcctc aacgaagggg gtcacttccg
     8161 tcaattgtga gccgatatgt ttgtgcactc ccaccacgtc gatgtgaccg agcgacgatg
     8221 caagcttgaa ttcgtccaag gctcgatcag ccgcaattcc gaacttgctt ttcttcaatc
     8281 cggtcgaaat atagggatgt gtcttggggt cgatgtctgg gttgatccgc aatgccacac
     8341 gcgctttctt tcccaccgat gcagcgacct cgttgagaac ctgcagttca gccgacgact
     8401 cgacgttgaa catgagaata tcggctttga gcgcatcgcg aatttcctca gcgctcttac
     8461 ccacgccggc aaacacgatc ttggacggcg gcacgcccgc cttcagggcg cgaaacagtt
     8521 cgccacccga gacaatatcg acgccgctgc cttccttcgc catgagacga aggatggcca
     8581 agttggaatt cgccttcatc gcgaaagcga tgatatgggg gattgctgca aacgcccggt
     8641 cgtagacctg aaaatggcgg acaagggtgg catggctata gatgtagcag ggtgtgccca
     8701 attccttgac gatccggctg accggcacct gctcgcaata cagctcaccc tgccgatact
     8761 cgaagctatg catcctgcaa atcctcactc aagaattcga gaccgctgag cgataaaccc
     8821 tgcagcgatg cttcaaaggc atccttgaat ttgcgatagc gcagcctcac atgcgggatc
     8881 gccgccttga tggcctccga atctatcaac gctttgaggg catccggggt cgcgagcttg
     8941 aggtgttcga aattcacgat cacgtccatc tttgcctgct cggcagcctg cttgatccgt
     9001 gcgagcagct cttgggcgtt cagccgatcc aatgtgccct ccagatccac gaagagcgct
     9061 gcaaacttgt catgccgttt cgtcttgatg ttgagcgcca gattctccgg cacgattgtt
     9121 ttgggatcga accgttgctt caacgcatgc agcgcgctgg gaatcaactg gtccgtatcc
     9181 agcacgggta gcttggcctg caaatttttc aacaccgacg ccaatggtga cagggagcct
     9241 ttggggcagg atcgtttgac cagcttacga aactcccgat tcatctccca tctcgccaca
     9301 agccgttgac ttgtgccgga gag
//
LOCUS       DATDNW010000034         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_139916, whole genome shotgun sequence.
ACCESSION   DATDNW010000034 DATDNW010000000
VERSION     DATDNW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139916"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VI359_00920"
     CDS             <1..317
                     /locus_tag="VI359_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY6972841.1"
                     /translation="KTLASIFRRTQVLLTTDSGPMHLAAAVGTAVVALFGPTAPWRTG
                     PYGPRHTVLRAGISCSPCFKKDCRTTDYEERACMKRLSVDEVVRAVLEKLAAVPISPS
                     SN"
     gene            335..1651
                     /locus_tag="VI359_00925"
     CDS             335..1651
                     /locus_tag="VI359_00925"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY6972842.1"
                     /translation="MRWHEQIVLLIYRTGFSLAIFLFVVLLFPFWLLIPKRRKTLFKR
                     LGWQDYPPPVQPSRRPIWIHALSLGEVLSAGSLIENVRRAIGDRPLYVSVSTLSAFAI
                     AAERFSRFCDGLFYFPYDVAFSVRRCLDRVEPALFVLVETDIWPGFIAELRRRRIPCL
                     LVNGRLSRESFRTYRFLRALFEPAFNTFHWVYPQSPGEAERFLGLGLEPARLRSTGNL
                     KFDVALSIPEEEALAALRQELNIPPDSRVMIAGSTHPGEEDMIRTVFSRLRAGFPSLR
                     LILVPRRPERGVEVQNVFRNDGVEVSLLSQPKGPPSVVVVVDRIGYLSRLYALADIAV
                     VGGSFVPKGGQNPIEPAACGKPVLFGPDMHDFPDVSRWLLEARGALQVQNGDELYTEC
                     HRLLADPQQARVMGDRARRVVTENQGTTAKIVADIVGLACGSSHSL"
     gene            complement(1769..2554)
                     /locus_tag="VI359_00930"
     CDS             complement(1769..2554)
                     /locus_tag="VI359_00930"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6972843.1"
                     /translation="MDVGAYRSLTGRTQEDSAAMRITTIVLGTLLTGLFLILPNLAQA
                     DQDLETAKLLIKMVELGRGVISEQQALINDPAKGDKGVTAEFVTSQIADRFKKQTNVD
                     LMAPNSHPSIKVLQTMLESQKDVMDGAQPMINKQGVGFKAFLPAVFARRTGATFFKKT
                     GIQVKLTSIAYRYDGNKPDDFEAEVLQVFADPKTPKGQVVSRTSMVNGKPALRVMKPE
                     YAGASCLSCHGNPKGERDITGMRKEGWNEGDLAGAISVVMPAK"
     gene            complement(2899..3405)
                     /locus_tag="VI359_00935"
     CDS             complement(2899..3405)
                     /locus_tag="VI359_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972844.1"
                     /translation="MSLCQRAIHLLLLTSLWAMTACALGDPSTGPLGVYLQSPQGSAE
                     WKNTEQQLAQAADAAATEYRRAKTLDALAAYETAVRACLDHGFLLYRVLDASSSEFPK
                     GFRQSLEDRASELMDIADEYLKLGASDVIAVAIARDVIHKYNVERMDRAQRRAEGILM
                     QYRYQRNY"
     gene            complement(3462..>3759)
                     /locus_tag="VI359_00940"
     CDS             complement(3462..>3759)
                     /locus_tag="VI359_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY6972845.1"
                     /translation="LRSTVESSNLEVVTLQGVVHLARRHQQQLGGKCGSIAFVLTDNT
                     GSIEVAIRRANRLLEPLREGDRVQVTAQIEVLRNPDNIPLRVCVEAVAIQHLGS"
BASE COUNT          736 a         1071 c         1093 g          859 t
ORIGIN      
        1 taaaaactct tgcgtcgata ttccggcgga cgcaggtgct gctcaccacg gacagcggtc
       61 ccatgcatct ggctgcggca gtcggaaccg cagtcgtcgc cctgttcgga ccgacggccc
      121 cctggcggac cggcccgtat ggcccgaggc atacggtgct gcgcgcgggg atcagctgta
      181 gtccctgctt caaaaaagac tgccggacca ccgactatga agaacgggcc tgcatgaagc
      241 gcctgagcgt ggacgaggtc gttcgggcgg tcctggagaa attggcggcg gtgccgatct
      301 caccctcttc aaactgacgg gagattcgtt gcaggtgcga tggcatgagc agatcgtcct
      361 tcttatctac cgcacgggtt tcagtctggc catcttcctg ttcgtggtcc tcttgtttcc
      421 gttctggttg ctaatcccga aacgccggaa gaccctgttc aagcgcttgg gctggcaaga
      481 ctatcccccg ccggtgcagc cctcccgcag accgatctgg atccacgccc tgtccctcgg
      541 cgaagtgttg tcggccggca gcctcattga aaacgtgcgg cgtgcgatcg gggatcgacc
      601 gctgtacgta tcggtgtcga ccttgtcggc ctttgccatc gccgctgagc gattcagccg
      661 attctgtgac ggactgttct acttccccta cgacgtcgcc ttttccgtga gaagatgcct
      721 ggacagagtg gaaccggcgc tcttcgtcct ggtggaaacc gacatctggc ccggattcat
      781 tgcggaactc cgacggagac ggattccgtg ccttttggtg aacgggcgcc tgtcccggga
      841 gtcgtttcgc acatatcggt tccttcgggc tctgttcgaa ccggcgttca atacgtttca
      901 ctgggtctat ccgcagtcac ccggtgaggc cgagcgcttt ctgggattgg ggctcgaacc
      961 ggctcggctg cgcagcacgg gcaatctcaa attcgacgtg gctctctcaa tacctgaaga
     1021 ggaagcgcta gcggcccttc gccaggaact caacattccg ccggattcac gtgtcatgat
     1081 tgccgggagc acgcatccgg gagaagaaga catgattcgc accgtgtttt cccggctgag
     1141 agcagggttt ccctcccttc ggttgattct cgtgcctcgc cgaccggagc gaggcgtcga
     1201 agttcagaat gtgtttcgga acgatggtgt ggaggtgtca ctgctctctc aaccgaaggg
     1261 cccaccctcc gtggtcgtcg ttgtggatcg gataggctat ctcagccggt tgtacgcgct
     1321 cgccgacatc gccgtggtcg gtgggagttt cgttcccaag ggcgggcaga atccgattga
     1381 accggccgcc tgcgggaaac ccgtcctctt cgggcctgac atgcacgatt ttccggacgt
     1441 gtcccgatgg ttgttggagg ccagaggggc ccttcaagta caaaatgggg atgaattata
     1501 cacagagtgt catcggctgc tagcggaccc gcaacaggcc agggtcatgg gagaccgcgc
     1561 ccgtcgcgtc gtgacggaga atcaaggcac caccgcgaaa atcgtcgcag acatcgtagg
     1621 ccttgcttgc ggctcctccc attcgctcta gtctcttccg ctaatctgaa tgttcccgat
     1681 gaaggtggtg attgatggag tgggtggccg ctcccccgtc gcaagctcac atgggggagc
     1741 ggtcgtggtg agtggcgcga aacgacgatt atttcgctgg catgacgacg ctgatggcgc
     1801 cggctagatc gccttcattc caaccttctt ttctcatccc ggtgatgtcc cgttctccct
     1861 ttgggttgcc gtggcagctg aggcaagagg ccccggcgta ttccggcttc ataacgcgaa
     1921 gcgccggttt gccattcacc atgctggtgc ggctgacgac ctgccccttc ggagtcttcg
     1981 ggtctgcaaa cacctgtagc acctccgcct caaaatcgtc cggcttattc ccgtcatacc
     2041 ggtaggcaat gctggttaac ttcacctgaa tacctgtttt cttaaagaag gtcgcccccg
     2101 ttcggcgtgc gaagacggcc ggcaggaacg ctttgaaccc gactccttgc ttgttgatca
     2161 tcggttgggc tccatccatc acgtcctttt gtgattccag catggtctgt aacaccttga
     2221 tcgagggatg ggaatttgga gccatgagat cgacattcgt ttgcttctta aaccgatcgg
     2281 caatctggct ggtcacaaat tcagcggtga cacccttgtc gccctttgca ggatcgttga
     2341 tcagggcttg ctgctccgag atcacgccac ggccgagctc gaccatctta atcagcagct
     2401 tggctgtctc aagatcctga tcggcctgtg ccagattcgg gaggataaga aacagtccgg
     2461 ttaacagagt tccgagtaca atagttgtaa ttcgcatcgc cgctgagtcc tcctgtgttc
     2521 gcccggtgag tgatctgtac gctccgacat ccatcgaagc aacctcctaa aggagtccgg
     2581 tcacgtcgca aaaatgtacg tgcaactacg agtatagcag gcgataaaaa actgtcttgt
     2641 cggggatgtc ttgatcgaat taccagaaca atccgggcta gaagggcgag tgggaataat
     2701 gaactaactc attgtaatac aatgcgttac ggtgtcttga ggcccatcat tgtgtgaggt
     2761 ttcccatttt tgataatccc ctaagaaccg agggggacct tcaagatact ggagggaatc
     2821 ggtacgacta ccggtactgg ccggcgaggt ctctgaccga cctatcagcg accattcgat
     2881 acatgcacct ctggtgggtt agtaattccg ctggtagcgg tactgcatga ggataccttc
     2941 ggctcgccgt tgagcgcgat ccattcgctc cacgttgtac ttgtgaatca cgtccctggc
     3001 gatggcgacc gcgatgacgt cactcgcgcc gagtttcagg tattcatcgg cgatgtccat
     3061 caattccgag gcacgatctt caagggactg tcggaatcct ttcgggaatt cagaagagga
     3121 cgcatcgagg acccggtaga gaaggaagcc gtggtcgagg caggcccgca cggctgtttc
     3181 ataagctgcg agtgcatcga gtgtcttggc gcggcggtat tccgtagcgg cagcgtctgc
     3241 agcctgtgcg agttgttgct cggtattttt ccattcagca gagccttgcg gcgactggag
     3301 gtacactccg agtggccctg tactcgggtc acccagcgca catgcggtca tggcccacag
     3361 agaggtaagc agcaacaagt ggatcgcacg ctgacagaga gacatcggct taccttcgtt
     3421 gagtcacgcg attatagctg aatttagggg gttctcgtac cttacgagcc gagatgctga
     3481 atcgcgaccg cttcgacaca gacacggagg ggaatattat cggggttacg caacacctca
     3541 atttgcgcgg tgacctgtac gcgatcgcct tctcgaagag gctcaagtaa gcgatttgcc
     3601 cgccggatgg ccacctcgat cgagcccgtg ttatcggtca acacaaacgc gatactccca
     3661 cacttcccac ccaattgttg ctgatgccgc cgagcaaggt ggacgacgcc ttgaagcgtg
     3721 acgacctcca ggtttgacga ctctaccgtc gagcgcagg
//
LOCUS       DATDNW010000035         6330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_754820, whole genome shotgun sequence.
ACCESSION   DATDNW010000035 DATDNW010000000
VERSION     DATDNW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754820"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="hpnJ"
                     /locus_tag="VI359_00945"
     CDS             <1..1432
                     /gene="hpnJ"
                     /locus_tag="VI359_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEY6972846.1"
                     /translation="NPPSFDMFDGGASSRYQATREIPSFWYPVWLAYSAGMIKDSRLL
                     DAPSHGVSPEETVALAKDYEFLALFTSTPGFKSDVRLAQMIKEVNPRIKIAFVGPHVT
                     IQPEQSLQASPAIDFVVRREFDYPLAEYASGKRLEEIAGISYRHNGQIIHTSDRPSIE
                     DLDALPFAVDVYKRDLDITRYNIPYLKHPYVAFYTSRGCPALCTFCLWPQTMSGHPWR
                     TRSIPNVVEEVRRCLEYFPQAKEIFFDDDTFPWAKDRTIDLCKALKPLKFTWSCNSRV
                     TAGYETLKAMKEAGCRLLIVGFESGNPDILMNIKKGATIEQARQFMKHCKELGIVVHG
                     DFQVGLPGETKETIEQTVQFAMELDPETIQVSMSHPYPGTEFDAYLKKHNYSVDHVMT
                     DEEGHQLPVFQYPGLSRKEIMHAVERFYDRYYFRPHVIFRILRRAIFNSHDRHRLYIE
                     AKEFLKVRAKRKGFVRSHIGAEASGS"
     gene            1490..1942
                     /locus_tag="VI359_00950"
     CDS             1490..1942
                     /locus_tag="VI359_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY6972847.1"
                     /translation="MMRTWTERTEIIALLGIAIVSQAIGNVFLSQGMKSISEGSPIIW
                     SNWLAVALQAVRTPSILFGVGFLIMFFVLFATTLSRADLSFVLPAISSEVIVNVACAS
                     YFLQETVSATRWLGVVLISIGVVLVVRSSPRTFGLENEPVAVRQEVNG"
     gene            1939..2307
                     /locus_tag="VI359_00955"
     CDS             1939..2307
                     /locus_tag="VI359_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972848.1"
                     /translation="MITALMIAVIVLADTAADVFLTKGMRQIGEVSSFAPRELLIVAG
                     RALSNHHVLASMVFMALHLTGFLIVLSWADLSFVFPATAMVYVVGTLAAKFILGETIS
                     AHRWTGTMLVCLGVGLVSIP"
     gene            2432..3586
                     /locus_tag="VI359_00960"
     CDS             2432..3586
                     /locus_tag="VI359_00960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6972849.1"
                     /translation="MNVPARHDSGTGQVRKVPAAKIQFLPEDRAWIAERIQEVLSTGQ
                     LTLGKYGAEFEQTFARFCGTRHAIAVNSGTSALEIILRTLDVEGKDVLVPTNTFFATA
                     GAVIHAGGRPVLVDMDPESFAVRPEDLEGKLTPNTAGLIVVHIGGIVSRRLPELQQWA
                     TKKGLWLVEDAAHAHGSSLNGVRAGAFGIAASFSFYPTKVMTSGEGGMIVTNDARIAE
                     EARIYRDQGKASFTQNAHIRMGYNWRMSEPHAIIGLKHLERLPDMIAERQKIASVYDR
                     ELRSFRGLGAVTVPAQGVCNYYKYLVVLRQKQDRKALKTLLRERFGVSLAGEVYEEPL
                     HKQPVFKAYVSGSLPVSEDYCSRHLCLPVFSGMEEADAYQVLHALRDTIG"
     gene            3604..4521
                     /locus_tag="VI359_00965"
     CDS             3604..4521
                     /locus_tag="VI359_00965"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6972850.1"
                     /translation="MAKVVVTGGSGFIGSHVVDALADAGHQITVIDHRVRPHRSDVGF
                     EDVDLMDLSSVLAATKEAEHIFHLAAVANVNYAYKYPVYSTALNVMGTTHILEAARIN
                     GAQRVYLASTVWVYNGAPNGKPADETVPFYLEGAGHIYTSTKMAAEMVCHNYAQLYKV
                     PFTILRYGIPYGPRMREELLIPIFIKKALNGQPLTISGRGEQYRNFVYVRDMAEAHVR
                     AMKDQAANQTYNLEGTRKVTVLEVAEGIKKVLGDDVKLEFVPARPGDFGGKEVTATKA
                     RQELGWYPTVSFEEGLRRTVDWFRQKWAN"
     gene            4762..6033
                     /locus_tag="VI359_00970"
     CDS             4762..6033
                     /locus_tag="VI359_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6972851.1"
                     /translation="MAESESDATNKIIGPPSPRQSDLPVLETEDQRAETKQAEAKEEA
                     LKSLPVRGGYSYIPLPAFAYNRNEKYWVGALMPVLRSNANDQVQDIFAPQYLHNQYIG
                     ETLSLNYYGYPSDTETYHAVVSYATKIERDFDLSYKNVAAGGGRYIVAGEVQWFKNAF
                     RRFFGIGNTAPESDETNYTSREAIVQFTAGINLTSDFALMLTERYHDVRVDQGAVSSL
                     PQTSSVFPTLTGISGAQIVGHKLSFRYDTRDKQLISTQGSYVNISAEFNQNLKHQEEN
                     QWWRFMFDARHLLPHGPGLVFVARAFMDSISGPGVPFYEEPTLGGETTLRAFGQSRFI
                     DENALLVNFEERIPVLDKRIFDHPINLEAAPFVDIGRVYDTFGTKSFTNLQVNPGIGL
                     RMLAPPNVVGRLDIGYGRDGTNVFVGLDYPF"
     gene            6089..>6330
                     /locus_tag="VI359_00975"
     CDS             6089..>6330
                     /locus_tag="VI359_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY6972852.1"
                     /translation="MMLRLHAAALLLFLALCSACATASLLNAAGDGDSKSVVEILQEG
                     VDVNVSFPIIGTRALMVAAAQGHVDAVKALLDAGADV"
BASE COUNT         1386 a         1699 c         1794 g         1451 t
ORIGIN      
        1 taatccccct tcatttgaca tgtttgatgg aggagctagt tcgcgatatc aagctaccag
       61 agaaattcct tcgttctggt atccggtgtg gctcgcctat tctgctggca tgattaaaga
      121 cagccggctg ctcgatgcgc cctctcatgg cgtaagccca gaagagacgg tcgccctcgc
      181 gaaggactac gagttcctcg cgctctttac cagtacgcca ggatttaaga gcgacgtacg
      241 gctggctcaa atgatcaaag aggtgaaccc taggatcaag atcgcctttg tcggtcctca
      301 tgtgactata caaccggagc agagtctcca ggcttctcct gccatcgatt tcgtggtacg
      361 gcgggaattc gactaccctc tggcagaata tgcctctggc aagaggttgg aggaaatcgc
      421 cggcatcagc tatagacaca acggacagat cattcacaca tcggatcgcc cgtccattga
      481 agatctcgat gccttgccgt ttgccgtgga tgtctacaag cgtgacctgg acattacccg
      541 gtacaacatc ccgtacctga aacatcccta tgtagccttc tacacctcgc gcgggtgccc
      601 ggcgctgtgt acgttttgcc tctggccgca gacgatgagc ggccaccctt ggcggacccg
      661 cagcattccc aacgtggtgg aggaagtcag acgctgtctg gagtatttcc cccaggcgaa
      721 ggagattttc ttcgatgacg acacgttccc ctgggccaag gaccgcacca tcgacttatg
      781 caaggctctc aagccgctga agttcacctg gtcgtgtaat tccagggtga cggctggata
      841 cgagaccctc aaggcgatga aagaagcggg gtgtcgcctg ttgatcgtcg gattcgagtc
      901 cggcaacccg gatattctga tgaatatcaa gaaaggcgcg acgatcgaac aggctcggca
      961 attcatgaag cactgcaaag agttggggat tgtggtgcac ggcgactttc aggtcggttt
     1021 gcctggcgag acgaaggaga ctattgagca gaccgtccag ttcgccatgg aactggaccc
     1081 ggagacgatc caggtttcga tgtctcaccc ctatcccggg accgagttcg atgcctattt
     1141 aaagaagcac aactattccg tcgatcacgt gatgaccgat gaggaggggc atcaacttcc
     1201 cgtgtttcag tatccgggcc tgagccgaaa agagatcatg catgcagtcg agcggttcta
     1261 cgatcggtac tacttccgac cgcacgtcat attccggatt ctgcgtcgcg ccatcttcaa
     1321 cagccacgac cggcaccgcc tctatatcga ggccaaggaa tttctcaagg tacgtgcaaa
     1381 gcggaagggg tttgtccgct cccacatagg agccgaagcg tcagggtcct gagctcccgg
     1441 agcagcttgg ctgactgcat gggaggaact gttgctgaaa ggatgctcga tgatgcgcac
     1501 gtggactgag cggacggaaa tcatcgcact tctggggatt gcaatcgtga gtcaagcgat
     1561 cggcaatgtg ttcctgagcc aaggcatgaa atccatctcg gaggggagtc caatcatctg
     1621 gagcaactgg ctagcggtcg ctctacaagc ggtccgcact ccgtcgattc tgttcggagt
     1681 cgggttcttg atcatgtttt tcgtgctctt cgccaccacg ctgtcccgcg cggacctcag
     1741 cttcgtgttg cctgcaatct cctccgaagt catcgtgaac gtcgcatgtg cgagctattt
     1801 tctgcaggag acagtctccg cgacacgttg gctcggagta gtcctcatct cgatcggcgt
     1861 cgtgctggtc gtgaggtctt ccccgaggac cttcggtctc gaaaacgagc ctgtcgcagt
     1921 acggcaggag gtcaacgggt gatcacagcg ctcatgatcg cggtcatcgt gttggcggat
     1981 acggcggccg atgtctttct gacgaagggc atgagacaga tcggcgaagt ctcaagcttt
     2041 gctcctcgag aattactgat cgtcgcaggg agagcgctgt ccaatcatca cgtgctcgcc
     2101 agcatggtgt tcatggctct gcatttgacc ggttttctga ttgttctctc ctgggccgat
     2161 ttgagtttcg tatttcccgc gaccgcgatg gtctatgtgg tcggaacgct cgcggccaag
     2221 tttatcttgg gagaaacgat ttccgcccat cggtggactg ggaccatgct cgtctgcctg
     2281 ggcgtcggat tggtgtccat cccatagatg gatacagcgt tggaagaaga cgcgcttcca
     2341 cgtgatttcg aagaacgcag ggtcttgcca atgagaaagg agcggtaacc aattgcaaac
     2401 tctgaagcag acacacagct cgcgcccgtc cgtgaacgta ccggcacgcc atgacagtgg
     2461 gacgggacaa gtaagaaaag tccctgctgc aaagattcaa tttctgcctg aagaccgggc
     2521 atggatcgcg gaacggatcc aggaggtgct gagcaccggc cagttgacgc tcgggaagta
     2581 tggagccgaa ttcgagcaga cgttcgcgcg gttctgcgga actcgacacg cgatcgccgt
     2641 caacagtggc accagtgcac tggagatcat acttcgtacg ctggacgtcg aaggcaagga
     2701 tgtcctggtg cccaccaaca cgttttttgc cacagctggt gcggtcattc atgccggcgg
     2761 acgtccggtt ctcgtagaca tggatcccga atcctttgct gtgcgcccgg aagatcttga
     2821 ggggaagctg actcccaata ctgctggtct gattgtcgtg catattggtg gcatcgtgtc
     2881 tcgccgtctt cctgagttac agcaatgggc aaccaagaaa ggcttatggc tggtcgagga
     2941 tgcggcccac gcgcatgggt cgtcgttgaa tggggtccgt gctggggcgt ttgggatcgc
     3001 cgcatctttt agcttttatc ccacgaaagt catgacttca ggagaaggag gcatgatcgt
     3061 aacgaacgac gcccgcattg ccgaagaggc tcgtatttac cgagaccaag gcaaagccag
     3121 ctttacgcag aacgcccata ttcgcatggg gtacaactgg cggatgtctg aacctcacgc
     3181 catcatcggg cttaaacacc ttgagcgctt gcccgacatg attgccgaac gtcagaagat
     3241 cgcttcggtg tatgaccgtg agttacgcag cttcagaggt ctcggggctg tgacagtccc
     3301 cgctcaagga gtctgtaatt actacaagta ccttgtggtg ctcaggcaga agcaggatcg
     3361 gaaggccttg aagaccttgc tgcgggagag attcggtgtc tcgctcgccg gagaggtcta
     3421 cgaagagccg ctccacaaac aaccggtctt taaagcctat gtgtccgggt cgctgccggt
     3481 ttcggaggac tattgctctc ggcatctctg cctgccagtg ttttcaggca tggaagaggc
     3541 agacgcgtat caagtgttgc atgcattaag agatacgatc ggctaaccaa aggagaggac
     3601 atcatggcaa aggtcgtcgt cactggaggc agcgggttca ttggatcgca tgtcgtcgat
     3661 gcattggcgg atgcgggaca ccagatcacc gttatcgatc accgcgtccg tccgcaccga
     3721 tcagacgtgg gttttgagga tgtagacctc atggacctgt catcagtcct cgctgcgacg
     3781 aaggaggctg aacacatttt ccacctagcc gctgtggcca acgtgaacta cgcgtacaaa
     3841 tatcccgttt attcaacggc actgaacgtg atgggaacga cacatattct ggaagctgcg
     3901 cggatcaatg gagcccagcg cgtgtaccta gcctcaaccg tgtgggtcta taatggcgcg
     3961 ccgaacggca agccggctga cgaaaccgtt cctttctatt tggaaggagc cggccacatc
     4021 tacacgtcta ccaagatggc ggccgagatg gtctgccaca actatgctca actgtataag
     4081 gtgccgttta cgattttgcg atacggcatt ccctatggcc cgcgcatgcg cgaggaactg
     4141 ctgatcccca tattcattaa gaaggcgttg aacggccagc cgctcacgat ttcgggcagg
     4201 ggtgaacaat accgtaactt tgtttacgta cgcgacatgg ccgaagccca tgtgcgtgcg
     4261 atgaaggacc aggcagccaa ccaaacctac aatctggaag ggacaaggaa ggtcaccgtc
     4321 cttgaggtgg ccgaggggat caagaaggtg ctcggcgatg acgtaaaact cgaatttgta
     4381 cctgcacggc ccggcgattt cggcggcaag gaggtcacag caacgaaagc tcgtcaagag
     4441 ctcggttggt atccgacagt gtccttcgag gaaggattac ggcgcactgt tgactggttc
     4501 cgccagaaat gggcaaactg aatccagtaa acgagcgagc gtgattgccc gacacaggag
     4561 ccgccaattc tccctctagt gttctggcgt cgcaggggta gcctcgaccg gccgtctctt
     4621 tggtgccttc gttttctccc tccgccactt ttcaaaccaa tcttgcgaag gttgggttgg
     4681 aatgagcctc tttgtgccct gggtaggctg gctcccctgt tcagggtgct ctgcttactg
     4741 cttctctcct caaactactg catggctgaa tctgagtcgg atgccacgaa caaaatcatt
     4801 ggtcctcctt ccccgcgaca gagcgacctt ccggtcctgg aaacggaaga ccagcgggcc
     4861 gagaccaaac aagccgaagc caaagaagag gccttgaagt ccttacccgt gcgtggtggg
     4921 tatagttata tcccgcttcc cgcctttgcc tataatcgaa atgaaaagta ttgggttggg
     4981 gccctgatgc cggtgttgag gtcgaatgcg aatgatcaag tccaagatat ctttgctccc
     5041 caatatctcc acaatcaata tattggcgag acgttatcgc tcaactatta cggatatccg
     5101 tcggacaccg aaacctatca tgcggtggtc tcgtacgcca ccaagatcga gcgagatttc
     5161 gatttgagtt acaagaacgt tgcggcaggg ggaggccgct atattgtcgc aggagaggtt
     5221 cagtggttta agaacgcatt tcgccggttt ttcggcatag gaaacaccgc tccggaaagt
     5281 gacgagacca actatacctc tcgagaggcc atcgtgcagt tcaccgctgg catcaatctc
     5341 acgtcggatt ttgccctcat gttgaccgaa cgctaccatg atgtcagagt ggatcaggga
     5401 gcggtgagct cgttgcccca aacgagttct gttttcccca ccctcactgg aatcagtgga
     5461 gcccagatcg ttggacataa actctctttt cgctatgata cacgggacaa gcagctgatc
     5521 tcgacacagg gcagctatgt gaacatctca gccgaattca atcagaactt gaagcatcaa
     5581 gaggaaaacc agtggtggcg gtttatgttc gatgcacggc atctgcttcc ccacggacct
     5641 gggttagtct ttgtggcccg tgccttcatg gactcgatca gcgggcccgg tgtgccgttt
     5701 tatgaagagc ccacgttggg cggagagacg actttgcggg cgtttggcca gagccggttc
     5761 atcgatgaga atgcgctcct ggtcaatttt gaggaacgga tccccgttct tgataagcga
     5821 atattcgacc atcctatcaa tctcgaagct gctccctttg tagacattgg gcgggtgtat
     5881 gacaccttcg gcaccaagtc cttcacgaat ctacaagtga atcccgggat tggcctccgg
     5941 atgctcgctc cccccaatgt tgttggtcga ctggacatcg ggtatgggcg cgatgggacg
     6001 aacgtcttcg tgggcctgga ttatccgttc taatctttac ggcattctgg gatttctctc
     6061 gtatgactgg cgtgatcggt gagactagat gatgctccga cttcatgctg ccgcacttct
     6121 gctctttctc gcactgtgtt cggcatgcgc gaccgcctcc ctgctcaacg ccgcggggga
     6181 cggtgattca aaatcggttg tggaaatcct ccaagaagga gttgatgtaa acgtgtcatt
     6241 ccccattatc ggaactcgcg ccctcatggt cgctgccgcg cagggccacg tcgatgccgt
     6301 taaggcattg ctcgatgcgg gagccgatgt
//
LOCUS       DATDNW010000036         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_618331, whole genome shotgun sequence.
ACCESSION   DATDNW010000036 DATDNW010000000
VERSION     DATDNW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618331"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VI359_00980"
     CDS             complement(<1..890)
                     /locus_tag="VI359_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972853.1"
                     /translation="MLKQSIGTVVVNNQRMVLVSFAVWFASGFLVWLGVSPSVGHAGN
                     QASPPPLFDNLGTLHHQITTTSEPAQRYFDQGLRLVYAFNHEEAILSFQEAARQDPQA
                     AMAYWGIALASGPNINAAMSKEDGRRAVDAIQKARAHAKTVSAAEQAYIDALSKRYLS
                     ARKATRASLNAAYAEAMRTVWRRYPDDPDAGILFVEALMNLRPWDLWTSDGKPKSGTA
                     ELVSVIEQVLEQHPEHPGACHYYIHALEASSHPEQALACADRLPGLMPGAGHLVHMPA
                     HLYMRLGKYHEAAERNEHAA"
     gene            complement(895..2235)
                     /locus_tag="VI359_00985"
     CDS             complement(895..2235)
                     /locus_tag="VI359_00985"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY6972854.1"
                     /translation="MSVAAKTWPKMTGRDEFQFLADLVLKRSTGDHTVVRLHDQHGGT
                     TRFANNQIVQNVDARRGLLSVTVAFERRQGTASTTDFTAGAIQETLARAEHIARLSPD
                     DPEYLPPVDQPEYLNVPTTRPETVAAGPARRLEYVNEAIGQCRMENLLAAGIVSSSIA
                     AVGIAASTGLFAHEERTEARFSLTVQAGDATGWASAAHRSIDHLKIQERTLAAIGKAK
                     RGGEPRELSPGRYKVILEPAAVAGLWTWLLWMLDAKSYEKGTSPVTGKLGRSIVDKRL
                     SLKNLPKHADLLGVSFTNDGLPSTESIWVERGELKQLSYDRFTARTHEIHTIPTLDAP
                     CLAAEDAEATNVRDLIKKTQRAILVTNFWYIRTVNATDLTLTGMTRDGTFLVENGEIV
                     SAVKNFRFHDSPLRVFNRVDACTGPAEAVTSETGKLLVPAMMVDDFHFSSVTKF"
     gene            complement(2235..>2567)
                     /locus_tag="VI359_00990"
     CDS             complement(2235..>2567)
                     /locus_tag="VI359_00990"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY6972855.1"
                     /translation="DLIVDVKKGIYIEGHGSYSIDQRRYNFQFGGDAFWLIERGKRTH
                     MVRDVVYHGITPEFWGTCDGVADQRHRRRFGFITCGKGQPGQSGWMTHAASPARFRGV
                     SVIRGGGN"
BASE COUNT          511 a          765 c          749 g          542 t
ORIGIN      
        1 tgggccgcat gctcgttccg ctcggcggcc tcatgatact tgccgaggcg catatagagg
       61 tgagccggca tgtggactag atgtccggcc cccggcatca ggccgggcaa tcggtccgca
      121 caggcgagcg cctgttcggg atgggacgag gcttcgagcg catgaatgta gtagtggcag
      181 gcgccgggat gctccggatg ctgctcgagc acttgctcga taacggacac aagttcggcc
      241 gtccctgatt tcggtttccc atcagatgtc cacagatccc acggtcgaag gttcatcaat
      301 gcctctacaa acaggatccc ggcatccgga tcatctggat atcgtcgcca gaccgttcgc
      361 atagcctcgg catacgctgc attaagagag gcccgcgtcg ccttgcgagc ggagagatag
      421 cgtttgctga gcgcatcaat gtacgcctgt tctgctgccg agaccgtttt ggcatgggcg
      481 cgagcctttt gaatcgcgtc gacggcgcgc cgtccgtctt ccttcgacat cgcagcattg
      541 atgttgggac cagaggcgag tgcgatgccc caataggcca tggcagcctg tggatcctgt
      601 ctcgctgctt cctgaaaaga aaggatggct tcctcgtgat tgaaggcata gaccaacctc
      661 aggccctgat caaagtatcg ctgagccggt tcggacgtcg tggtgatttg atgatgcagc
      721 gtccccaggt tgtcgaacaa cggaggaggg cttgcctggt tccccgcatg accgaccgat
      781 ggggaaacgc caagccagac aaggaaccca gaggcgaacc agaccgcgaa tgagacgaga
      841 accatccgct ggttgttcac gacgaccgtt ccgatcgact gtttcagcat cctgctagaa
      901 ctttgtcacg ctggagaagt ggaaatcgtc gaccatcatg gcgggcacca gcaacttgcc
      961 cgtctcagag gtgaccgcct cagcgggacc ggtacaggcg tctacccggt tgaagacgcg
     1021 caagggacta tcgtgaaatc ggaaattctt taccgcggag acaatttcgc cgttctccac
     1081 gagaaacgtg ccgtcccgag tcatgccggt caacgtgaga tccgtcgcat tcaccgtgcg
     1141 aatgtaccag aagttcgtca caagaatcgc gcgttgtgtt ttcttgatga gatcccggac
     1201 attggttgct tccgcatcct ccgcggcaag gcatggtgcg tccaatgtcg ggatcgtgtg
     1261 aatttcgtgc gtcctggccg tgaagcgatc ataggagagt tgtttcagtt ccccgcgttc
     1321 aacccaaata gactccgtgg agggtaatcc gtcgtttgta aagctcaccc ccaagagatc
     1381 ggcgtgcttc gggagattct tcaggcttaa tcgtttgtcg acgatcgatc gcccgagctt
     1441 gcctgtgacg gggctcgtgc ctttctcata agacttggca tcaagcatcc atagcaacca
     1501 tgtccagagc cccgccactg cagccggttc gaggatcact ttgtatcgac cgggagacag
     1561 ttctctcggc tcccctcctc gcttggcttt accgatcgcg gcgagtgtgc gttcctggat
     1621 tttcaaatga tcgatcgacc ggtgcgccgc cgaagcccag cccgtcgcat cgcccgcctg
     1681 cacggtgagg ctgaagcgcg cctcggttcg ttcctcgtga gcaaagagac ccgtgctggc
     1741 cgcaatccca acggccgcaa tcgacgatga cacgatgccg gccgcaagga gattttccat
     1801 ccggcactga ccaatggcct cgttgacata ctcgaggcgg cgagccggtc cggctgcaac
     1861 cgtttccgga cgagttgtcg gaacgttcag atattcgggc tggtccaccg gaggaagata
     1921 ttcgggatcg tcgggggata ggcgagcaat gtgctcggcg cgggcgagtg tctcctggat
     1981 tgctccggct gtgaaatcgg tggtgctcgc cgtaccctgc cgacgttcaa acgccacggt
     2041 cacactgagc aaacctcgtc tggcgtcgac attttgcacg atctgattgt tggcaaaccg
     2101 ggttgtcccc ccatgttgat cgtgcaggcg cacgacggtg tgatcgccag tggatcgttt
     2161 cagcaccaag tcggcgagaa actgaaactc gtcgcgaccg gtcatcttcg gccacgtctt
     2221 ggcggcgacg ctcatcagtt cccgcctccc ctgattacgc tgacccctcg gaatcgcgcg
     2281 ggggaagccg catgagtcat ccagcccgat tggcccggct ggcccttgcc acacgtaata
     2341 aacccgaacc ggcggcgatg ccgctggtcc gcaactccgt cgcacgtgcc ccaaaactca
     2401 ggcgtaatgc cgtgatacac gacatcgcgg accatatggg tccgcttacc acgttcgatg
     2461 agccagaacg catcgccgcc gaactgaaag ttgtagcggc gctggtcgat gctgtatgac
     2521 ccatggcctt caatgtaaat ccccttcttc acgtcgacga tcagatc
//
LOCUS       DATDNW010000037         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_345167, whole genome shotgun sequence.
ACCESSION   DATDNW010000037 DATDNW010000000
VERSION     DATDNW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345167"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2416
                     /locus_tag="VI359_00995"
     CDS             143..2416
                     /locus_tag="VI359_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972856.1"
                     /translation="MTDGHLASHDLIAQLGPLCQGIDNEIIRDFVSRMDPEYFTQVEV
                     ETVAQHLHFIAALTLDHPCELSIKPREGGRYEITVVAYDYFSEFATICGLLSTFGLNI
                     EEGRIYTSAEVPSSAQKPVRTPWPTRRPKVRPGLSRKKIVDVFVVRPASDHPFTPSHQ
                     VRLRKELTSMIQLLDAGNFEEARQQVNRRLVEQLGKRRASFTGLLHTVQITFDNSQSP
                     TDTIMDIRSDDTPAFLYAFANALAMRNVYITKARFEVDGGKLHDRFHVRNRHGQKLTD
                     QAEQQQLRLTAVLIKQFTHALTWAPDPAKALESFDQFLDVLVDETKGKGKKQGLDVLS
                     DKTTFPLLARLLGASDFLWEDFLRRQHGNLLPLLQGYRNAPLIKPQAILRKELDRVVN
                     RAKTDSMRKEALNRFKDQELFRIDMKHIVEADTSLADFSLALTELAEVILDRSLKDCQ
                     AKLNKQYGVPHLTNRKPCPFAILGMGKFGGRELGYASDIEVLFVYGGAGRTSGKQGIE
                     NSEYFERLAQELLQWIEAKQEGIFHLDVRLRPHGGKGLLTNPLDEITSYYSEKGLAAP
                     FERQALIKLRHVAGDTSLGKQVEAHRDHFVYSGAPWDLDTALDLRRQQITQLVERGTV
                     NVKLSPGGVIDIEYAVQYLQVMHGHTQPVLRTSNTLQALAALVECGIVTRQDGDNLRK
                     AYLFTRMLIDGLRMVRGNAKDLVLPPADSDEFIFLARRVGYTTDDWQAGARHLQSDIE
                     RHMEETKRFFERTFGKL"
     gene            2844..>3562
                     /locus_tag="VI359_01000"
     CDS             2844..>3562
                     /locus_tag="VI359_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972857.1"
                     /translation="MAVSLSIPLQTRQMLRDLGTYIATRNVLATQILEIDENICLKNQ
                     EPKNKKISQLVSSSCFEGKKQNSALDALKALEVFKDITVIEKARILGYSADFSIYRWG
                     LRFHNLVESSGGKIEDLTLERARELAKYEFPEMADYKDLFYRHELLSTPWTLLPVRLD
                     RAVTLVELGLAFSLAYFWVFLQEATRSGTFEQEETIFSAFSRTRVSRFLFFVFAATPP
                     VVACRLTIESYYAGTPLTKLP"
BASE COUNT          843 a          976 c          967 g          776 t
ORIGIN      
        1 gtcagggagc gtacgtggag ggacagaagg aggctgcgaa cggctcttct tcggttgggt
       61 gatccggcgc accatgagcc ggcattatac tgacgcatgg atcaccgcac aatgaaggga
      121 cttcgggtat actgccgtca ctatgaccga tggacacctc gcttcccacg atctgatcgc
      181 gcaacttggt cctctctgcc agggcatcga caacgagatc attcgtgact tcgtctcgcg
      241 gatggacccc gagtatttca cgcaggtgga ggtcgagacc gtcgcacagc atcttcactt
      301 catcgccgcc ctgacgctcg atcacccctg cgagctgtcg atcaaacctc gcgaaggcgg
      361 ccgttacgag attaccgtcg tggcctacga ctacttttcg gagttcgcga cgatctgcgg
      421 acttctgtcc acatttggct tgaacatcga agaaggtcgc atctacacct ctgccgaggt
      481 cccctcttcg gcccagaagc ccgtacgcac gccttggccg acccgacgac caaaggtcag
      541 gcctgggctg agtcggaaga agattgtcga tgtgttcgtg gtccgtcccg cctccgatca
      601 ccccttcaca ccatcccatc aggtccggct gcgcaaggaa ttgaccagca tgatccagct
      661 cctggatgcc ggcaactttg aggaagcccg gcagcaagta aatcgacgtc tcgtggagca
      721 actcggtaaa cgacgcgcgt cgtttaccgg cttgctccat actgtccaga tcacgttcga
      781 taatagtcag tcaccgaccg acaccatcat ggacatccgt tccgacgata ccccggcctt
      841 tctctatgcc ttcgcgaatg ccctggcgat gcgcaacgtc tacatcacga aggcgagatt
      901 cgaggtcgac ggcggcaagt tgcacgaccg attccacgtc cgcaatcgcc acggccagaa
      961 actgaccgac caggccgagc agcaacagct ccggctgacg gcggtcttga tcaaacaatt
     1021 cacgcacgcc ctcacgtggg cccccgaccc ggcgaaggcg cttgaatcgt tcgaccaatt
     1081 cctcgatgtg ctcgtggacg aaaccaaagg aaaagggaag aagcagggcc tcgacgtcct
     1141 gagcgacaag acgacgtttc cgctgctcgc ccgcctgctg ggagccagcg attttctatg
     1201 ggaggatttt ttgcgccggc aacatggaaa cttgctgccg ctgttgcaag gctaccgcaa
     1261 cgccccgctc atcaaaccac aggcaatcct ccgcaaggaa ctcgatcgcg tggtgaatcg
     1321 cgcaaagacc gacagcatgc gtaaggaggc gctcaatcgg tttaaggatc aggaactctt
     1381 tcgtatcgac atgaaacaca tcgtcgaggc ggacaccagc ttggccgact tctcgctcgc
     1441 actgaccgaa ctggccgaag tgatcctgga ccggagtttg aaagactgcc aggccaagtt
     1501 gaacaaacag tatggcgttc cccatttgac gaacaggaag ccctgcccct ttgcgatcct
     1561 gggcatgggc aagttcggcg gccgggaact tggttacgcc tcggacatcg aagtgctgtt
     1621 cgtctacggc ggcgcgggtc gcacgagcgg caagcagggc atcgagaata gcgaatattt
     1681 cgagcggctc gctcaggaac tcttgcagtg gatcgaagcc aaacaggaag gcatctttca
     1741 tctcgacgtg cgtctgagac cacacggtgg caagggattg ctgacgaatc ccctcgacga
     1801 aatcacgagc tactacagtg aaaaggggtt agccgctccc ttcgagcgac aagccttaat
     1861 taaattgcgg cacgtggcgg gagacacctc cttgggaaaa caggtggaag cccatcgcga
     1921 tcatttcgtc tatagcggcg cgccgtggga ccttgacaca gcgctcgacc tgcgccgtca
     1981 gcagatcacg caactggttg aacggggaac cgtcaacgtg aaactcagcc ctgggggcgt
     2041 catcgacatc gagtatgccg tgcaatacct ccaagtcatg cacggtcata cgcagcctgt
     2101 cttacgcaca tcgaataccc ttcaggcgct cgcagcgctc gtcgaatgcg ggattgtgac
     2161 gcggcaggat ggcgacaatt tgcgcaaggc ctacttgttt actcgcatgc tgatcgacgg
     2221 gttgcggatg gtgcgaggca acgcgaagga tttggttctc ccgcccgccg attcagacga
     2281 gttcatcttt cttgcacggc gtgtggggta taccactgat gactggcaag caggggcgag
     2341 gcatttgcag agtgatatag aacggcatat ggaagagaca aagagatttt ttgaaagaac
     2401 gttcggaaag ttatagggac tggcacccgt gtcgttcctg tgctcgcgga gtgcgcacgc
     2461 gggaataatc acattgcaaa attgcgggcg gtgctccctc catgcgcgca gtaggatcga
     2521 cacaggcgcc agtcctgggg catatctcat ctgccggtgg ggagagaggg gaaacgagca
     2581 agcttgcagg gagcattaat attgcaagtc gaatgcacag ccaccgtttc tgccatagct
     2641 gggagagagg acaactagca atttgctgtc ggttgatgcg cacttagttt gatggacgag
     2701 cgcaatccta aaaagtagaa tgtcccccat tccagaatcc cacccctttt ggctgatacc
     2761 aaaggagcga ccttacaatg acaagaacag attcagccgt ctcatgtgcc cgatccggtt
     2821 tggtcatagt atttcttttg tttatggctg tttcattaag tatcccccta caaacgcgcc
     2881 agatgctacg cgacctaggg acgtatattg ctacacgtaa tgtgttggcg acccaaatat
     2941 tagagattga tgaaaacatt tgcctgaaaa accaagagcc caagaataag aagatctcgc
     3001 agctggtttc atccagctgc tttgaaggaa agaagcaaaa cagcgccctt gatgccctaa
     3061 aagctttgga ggttttcaag gacataacag ttatagagaa ggccagaatt cttggctatt
     3121 cggcagactt ttcgatttac cgatggggat tacgtttcca caacctagtg gagtcatcag
     3181 gcggcaagat cgaagatttg actctagaac gagcaaggga actagccaaa tatgaatttc
     3241 cagaaatggc tgactataag gatttgtttt atcgtcatga gctcttgtcc acaccatgga
     3301 cgctgctccc cgttagactt gatcgtgccg taactctcgt agaactcggg ctagcgtttt
     3361 cactggctta tttctgggtg tttctccaag aagctactcg gtcagggact tttgaacagg
     3421 aagaaaccat ctttagtgcc ttcagtagaa ctcgcgtgag tcgtttcttg ttcttcgtct
     3481 ttgctgctac acctccggtt gtggcgtgca ggctaacaat tgaatcgtat tatgccggta
     3541 ctccattaac aaaactgcct tt
//
LOCUS       DATDNW010000038         6580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_550182, whole genome shotgun sequence.
ACCESSION   DATDNW010000038 DATDNW010000000
VERSION     DATDNW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550182"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VI359_01005"
     CDS             <1..216
                     /locus_tag="VI359_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HEY6972858.1"
                     /translation="LLKQVDAGAAGPREQVKFIKMDNVRIIDGPFLGFNGLVDEVDQD
                     HNRLKVMVSIFGRSTPVELGFLQVERI"
     gene            246..671
                     /gene="rplK"
                     /locus_tag="VI359_01010"
     CDS             246..671
                     /gene="rplK"
                     /locus_tag="VI359_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY6972859.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKEGDSIIPVIITVYKDRSFTFILKTPPASDLLKKAAGIIKGSGVPQKDKVGKITK
                     TQLREIAQKKMSDLNAADLEGAIKIIEGTARSMGVVVQG"
     gene            707..1399
                     /gene="rplA"
                     /locus_tag="VI359_01015"
     CDS             707..1399
                     /gene="rplA"
                     /locus_tag="VI359_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY6972860.1"
                     /translation="MGKKKRAAAEKLQPRLYALREAVDAVKGAAYAKFDESVDLAIRL
                     GIDPKRSDQMVRGTTSLPHGTGKKLRVLVFAKGEKEQEARQAGADFVGSDDLMEKIKG
                     GWMDFDCAIATPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVAEIRKGRVEYK
                     VEKAGIVQVPVGKVSFKADQLFDNASAVLEAVIKAKPASCKGRYLKSVTISSTMGPGV
                     KLDAMALAKQWS"
     gene            1437..1943
                     /gene="rplJ"
                     /locus_tag="VI359_01020"
                     /pseudo
     CDS             1437..1943
                     /gene="rplJ"
                     /locus_tag="VI359_01020"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            1971..2375
                     /gene="rplL"
                     /locus_tag="VI359_01025"
     CDS             1971..2375
                     /gene="rplL"
                     /locus_tag="VI359_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY6972861.1"
                     /translation="MSATASKLSQEELIKAIESMSVLELAELVKGLENKFGVTAAAPV
                     AVAAAPAAGGGAAAAAAEEKTAFDVILASAPADKKIQIIKVVRELTSLGLKEAKDLVE
                     GAPKPVKTGVTKEESDTMKKKLEESGAKVEVK"
     gene            2456..6412
                     /gene="rpoB"
                     /locus_tag="VI359_01030"
     CDS             2456..6412
                     /gene="rpoB"
                     /locus_tag="VI359_01030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY6972862.1"
                     /translation="MSETTLQEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQMEVE
                     PERRKDYGLQAALASVFPIPDYNNTAVLEYTSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGYSSDDLLKMYYPVEEIRVVKGKLMRKLDPEIHHGLRCSAEVTEKGSKEPLVREGGR
                     LTKGLIAKLKSAGVKEIPLSPAELVGRAVLTELVDSKKHKIAEKNQRLTAEIVEKITE
                     SDIEDFKVIYLDTATATAVILDTLEMEKTGSKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLELDLPLEQRTLTAQDIVEVIRYLVNLKMGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINQFGFIEAPYRKVVKGRVTDEIEFLSAIEGDKYIIA
                     QANSKLDGSNRLVSETISARSGGDFVTATPDKIEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLLKSEAPLVGTGMEAVVARDSGYVVQAKRTGVVESVDATRIV
                     VRADSKEGRRGKDSGLDVYDLIKFQRSNQNTCITQTPVVRIGQPVKKGQVLGDGPAID
                     RGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGKE
                     DITRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLTVPPGVEGIIVDVKIFSRKGLDKDERSKTIESEDQMKLQRDHHEEL
                     RIIDEEKTKKIRKLLLGKVVGRDLMDPESGDVILKKKGKLTAEILKRLPDETVRYIIL
                     SDPEEQKELEDIERRAKEQIEILQTLYDEKVGRLKRGDELPPGVIKLVKVYIAMKRKI
                     QVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLG
                     WAAKALGITVASPVFDGASEKEIKDLLKKGKLPTSGQSQLMDGKTGEPFGSPVTVGYM
                     YVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQ
                     EFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKSQE"
BASE COUNT         1633 a         1583 c         2074 g         1290 t
ORIGIN      
        1 ttgctgaagc aagtggatgc cggtgcggcc ggcccgcggg agcaagtcaa attcatcaag
       61 atggacaacg tgcgcatcat cgatgggccg ttcctgggat ttaatggcct cgttgatgaa
      121 gtggatcagg atcacaatcg cctgaaggtc atggtcagta ttttcggacg atcaacgcct
      181 gtcgaactcg gatttctaca ggtcgagcgg atttaagaag caaagaaaaa gaataggggt
      241 agaacatggc caaggaagtt tcagcgcaaa tcaagctgca gattcctgca gggaaagcca
      301 atccggcgcc tccagttggg ccctcccttg gacagcacgg tgtcaatatt atggaattct
      361 gcaagcagtt caatgccaag actcagaagg aaggcgacag catcatcccg gtcattatta
      421 cggtctacaa ggatcggagt ttcacgttta ttctcaagac accccctgcg tctgatctgt
      481 tgaagaaagc cgctggaatc attaaagggt ccggcgtgcc gcagaaagac aaggtgggca
      541 aaataaccaa gacccagttg cgcgagatcg cgcaaaagaa gatgtcagac ctgaatgccg
      601 ctgatcttga aggcgctatc aagatcatcg aaggaacggc gcggagcatg ggggtcgtgg
      661 tccaaggata atagctgttc gagagagtgg gaaggagtaa ggggaaatgg ggaagaaaaa
      721 gcgagcggcg gcggaaaaac tccagccgcg tctgtatgcc cttcgtgaag ccgtcgacgc
      781 ggtgaagggg gcggcctacg caaagtttga tgagtctgtc gatttggcga ttcggttggg
      841 catcgatccg aaacgttctg accagatggt gcgagggacg acctccttac cccatggaac
      901 ggggaaaaag cttcgtgtgc tggtcttcgc caagggcgag aaagagcagg aggcgaggca
      961 agcaggggcc gatttcgtgg ggtcggatga cctgatggaa aagatcaaag gggggtggat
     1021 ggacttcgat tgcgcgattg ccacgccgga tctgatggca tcggtcggta agctcgggaa
     1081 ggtgttaggg ccgcgcggtt taatgccaaa tccgaaaacc ggaacggtca cgttcgaagt
     1141 tggtaaagcc gtcgcggaaa tccggaaggg tcgcgtcgaa tataaagtgg aaaaggccgg
     1201 catcgtccaa gtgcctgtcg ggaaggtgtc gtttaaggcc gaccagttat tcgacaatgc
     1261 ctctgcggtg cttgaggcag tcattaaagc aaagccggcc tcctgcaaag gacgctatct
     1321 aaagagcgtg acgatctcca gcacgatggg cccgggtgtg aagctcgatg cgatggcgtt
     1381 ggcgaagcag tggagttagt cgcggtctga ccgttgtacg gagaaagggt taggtcatga
     1441 agaaggaaga gaaatcgacc gcggtcgcgg agttgaccga gaagttcggc cgcgctcggc
     1501 tcgccattct cacggaatgc gtcgggctgc ctgtcaatca gattacggag ttgcggaagc
     1561 aattgcgcgg ggcaaaggcc gaatacaggg tagtgaaaaa caccctggca gcccgtgccg
     1621 ccgaaggaac caccatctca ggtgtgaaag ggtacctcaa aggaccaacg ggaatggtga
     1681 tcgggtatga cgatcccgtg ctgccgacca aggtgctccg ggattttata ctggcggaga
     1741 agcgggaaga gaaaatcaag attaccatcg gtgttttgga agggaaggtg ttgcagccgg
     1801 ccgagcttac ggcggtcgcg aagttgccga agagggaagt gttgatcgcc atgctgctgt
     1861 cggcaatgca ggggccggca cgtgggttgg tctatgcctt gagcgcggtc ttgtcgaaat
     1921 ttgtcagggt cattgcagcc attcaggata aaaagaaagg ggagggggac atgtcagcaa
     1981 cagcgtcaaa actatcacag gaagaattga tcaaggccat cgagagcatg agtgtgctcg
     2041 aattggctga gctcgtgaag ggactggaga ataaattcgg cgtaactgcc gcggctccgg
     2101 tcgctgtcgc cgcagctcca gctgctggcg ggggcgcagc cgctgcggct gccgaagaga
     2161 agacggcctt tgacgtcatt ctcgcgtcgg ctccggcaga caagaagatt cagatcatca
     2221 aggttgttcg cgaattgacc agcctgggct tgaaggaagc gaaagattta gtggaaggcg
     2281 cgcccaaacc ggtgaagacg ggcgtgacga aagaagaatc cgacaccatg aagaagaagc
     2341 tcgaagagag cggcgcgaaa gtcgaggtta agtaggctcg acgcagtagt aaatccgggg
     2401 gtgcctgtct tctccaggca tccaagccca accatatgta gtggaggact gccgaatgtc
     2461 cgaaacgact ctacaagagt tcgtggaacg gaaagacttt tctcgcattc gcacgagcat
     2521 cgatattccg gatctgatcg agatccagaa gcgctcgtac gaagagttct tgcaaatgga
     2581 agtggaaccg gagcgccgca aggactacgg tcttcaagcg gcactggcga gtgtctttcc
     2641 gattcctgat tacaacaaca cggcggtgtt ggaatacacc agttataccc tggggacacc
     2701 caaatacgac gagcgggagt gtcttgagca ggggatgacc tttgctgtgc ccctcaaact
     2761 ccgcgtccgg ctggtggtgt tcgacaagga agacaagggg ccgaagaaaa aagtgctcga
     2821 tgttcgtgag caagaggtct atgtcgggga actgccgctg atgaccgagc ggggcacgtt
     2881 tatcgtgaat ggcaccgagc gcgtggtcgt gagccagttg caccggtcac ccggtgcgtc
     2941 gttcacccac gacaagggac ggacccatgc gagcggcaaa gtgttgtact ctgcccgcat
     3001 cattccgtat cgaggttcct ggctcgattt cgaattcgac gcccgcgaca ttctctacgt
     3061 tcgcatcgat cgccgtcgaa aaatgccggc gacgatcctt ctgaaagcgt tcggatattc
     3121 cagtgacgat ctcttgaaga tgtactaccc ggttgaagaa atccgtgtgg tcaagggcaa
     3181 gttgatgagg aagctggacc ccgagatcca tcacggtctg cgatgttcgg cggaggtcac
     3241 agagaagggt tcgaaagagc cgttggtgcg ggaaggggga aggctgacca agggtctgat
     3301 cgcgaagttg aaatcggccg gggtaaagga aattcccctg tcgccggctg agctggtcgg
     3361 tcgagccgtc ttaaccgaac tcgtcgattc gaaaaagcat aagatcgcag agaaaaacca
     3421 acggctgacg gcggagatcg tcgagaaaat taccgagagt gatattgaag acttcaaggt
     3481 tatttacttg gacactgcga cggcgaccgc ggtgattctg gataccttgg agatggagaa
     3541 gaccggttcg aaagaagagg cgatggtcga aatctatcgc cgactgcgtc cgggcgaaac
     3601 gccatcggtc gaaacggcgc gggcgctatt cgacaatctg ttcctcaact ccaagcggta
     3661 cgacctgtca cccgttgggc gtctgaagct gaacaaaaaa ctcgagctgg atttaccact
     3721 ggaacagcgg acgttgacgg cacaggacat tgtcgaagtc atccgctacc tcgtcaatct
     3781 gaaaatgggc aagggcgaga tcgatgacat cgaccatttg ggaaatcgtc gagtccgatc
     3841 cgtgggcgag ctcttggaaa atcagttccg cctcggcctg gtccgcatgg agcgtagtat
     3901 caaagagcgc atgaacctgc tcgatatgga aaccgtgctg ccgcacgacc tcatcaacgc
     3961 caagccggtc gtggccgcgg tcaaagaatt tttcagcagc agccaactgt cacaatttat
     4021 ggatcagaca aatccgctcg cggagatcac gcacaagcgg cgcctctctg cattgggtcc
     4081 tggcggattg acacgtgaac gagcgggctt cgaagtgcgc gacgtccatc cgtcacatta
     4141 cagccgtatc tgtccgatcg agacaccgga aggtccgaac atcggcctga tcacgtcatt
     4201 ggcgacctat gcgcggatca accaattcgg gtttatcgaa gcgccttatc ggaaagtggt
     4261 caagggaagg gtgacggacg aaatcgagtt tctgtccgca atcgagggcg ataaatacat
     4321 cattgcccag gcgaactcga aactcgacgg ttccaatcga ctggtctcgg agaccatctc
     4381 ggccagatcc ggcggcgatt tcgtgacggc gactcctgac aagatcgaat acatggacgt
     4441 gtcgccgaaa caggtggtca gcgtggcgac tgcgttggtc ccgtttttgg agcacgacga
     4501 cgccaaccgg gctctgatgg gatcaaatat gcaacgccaa gccgtgccgc tgttgaaatc
     4561 ggaagccccg ttggtcggca ccgggatgga agcagttgtc gcgcgtgatt ccggctatgt
     4621 ggtgcaggcc aagcggaccg gcgtcgtgga aagcgtcgat gcgacgcgta tcgtcgtgcg
     4681 cgcggattcc aaagaaggcc ggagagggaa agattcgggt ctggatgtct atgaccttat
     4741 caaattccag cgatcaaacc aaaacacgtg tatcactcaa accccggtcg ttcggatcgg
     4801 tcagccggtc aagaagggcc aagtgttggg tgacggtcct gcgatcgatc gaggtgagct
     4861 tgccttgggc aagaacgtcc tcgtcgcgtt tatgccgtgg ggtgggtaca acttcgaaga
     4921 cgcgattctg ttgagcgaga agcttgtgcg ggaagatgcg ttcacctcga ttcacatcga
     4981 agagttcgag gtcgaagcgc gcgatacgaa gctgggcaag gaagacatca cgcgagacat
     5041 tcccaacgtg ggagaagagg cgctgcggaa tctcgatgag agcggcatca tccgtatcgg
     5101 cgccgaagtg aagccggggg atattctcgt ggggaaggtc acgcccaaag gcgaaacgca
     5161 actgactccg gaagaaaaac tcctccgtgc catctttggc gagaaggccg gagacgtcaa
     5221 ggacacgtcg ttgacggtgc cccctggtgt cgagggcatt atcgttgacg tcaagatctt
     5281 ctctcgcaaa ggcctcgaca aggatgaacg gtccaagacc atcgagagcg aagatcagat
     5341 gaagctgcag cgcgaccacc atgaggagct gcggatcatc gacgaggaaa aaacgaagaa
     5401 gattcgcaag ttgctcttgg ggaaagtcgt cggccgggat ctgatggatc cggagagcgg
     5461 tgatgtcatt ctcaagaaga agggcaagct cacggccgaa atcctcaagc ggcttccgga
     5521 tgaaaccgtc cggtacatca tcctgagcga tccagaagag cagaaggagc tcgaagacat
     5581 cgagcggagg gcgaaggagc agatcgaaat tctccaaacg ctctacgacg aaaaggtcgg
     5641 tcgcttgaag cggggtgacg aacttccgcc gggcgtcatc aagctggtga aggtgtacat
     5701 tgccatgaag cggaagattc aggtcggcga caagatggcc ggccgccatg gcaataaagg
     5761 ggtggtgtcg cgcgtgttgc ccgaggagga catgccgtat ttgccggatg ggactccggt
     5821 ggagatcgtg ctgaatccgc tgggcgtgcc atcccgtatg aacgtcggtc agattctgga
     5881 aacacacctc ggatgggcgg ccaaggcgct tggaattacc gtcgcgagcc cggtgttcga
     5941 cggggcttcc gagaaagaga tcaaggattt gctgaaaaag ggcaaattgc ccaccagcgg
     6001 gcaatcgcag ctcatggatg gtaagacggg agaaccgttc gggagtccgg tcacggtcgg
     6061 ctacatgtat gtgctgaaat tgcaccactt ggttgacgac aagattcacg cgcgctcgat
     6121 tggaccctat tctctcgtaa cgcaacagcc gctcggtggg aaagcccaat tcggcgggca
     6181 gcggctgggg gaaatggagg tctgggcttt acaggcctat ggagccgcct cgacactcca
     6241 ggaatttttg accgtgaagt ctgatgatgt gccgggccgg tctcgtatgt acgaagcgat
     6301 agtcaaaggt gagccgttct tggagccggg attgccggag tcgtttaacg tattggtcaa
     6361 agaacttcaa agtttggggc ttgatgtcga gttggttaag tcacaggaat aaattgaaat
     6421 cgaatgacga gtgacattca cggcatgcga tctctttttg agccgaacaa tctgtcactc
     6481 aaactagtca ctcctctcta agtaaggagg gatacacctt ggaaggcgtc tatacattgt
     6541 ttgaaaagcc gcgggattcg gtctccttcg atgcgatgcg
//
LOCUS       DATDNW010000039         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_824418, whole genome shotgun sequence.
ACCESSION   DATDNW010000039 DATDNW010000000
VERSION     DATDNW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824418"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1908
                     /locus_tag="VI359_01035"
     CDS             406..1908
                     /locus_tag="VI359_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972863.1"
                     /translation="MLIATVDKFAQMPWNGDVQALFGFVDRECDRCGFLTPATEHVTA
                     HRSSGKKTGSSAVRESERLAPPDLIIQDELHLISGPLGTLVGLYETAVDFLASRRERG
                     RLVGPKVVASTATVRRAFEQVQAVFQRNLRVFPPPGLEPEDSFFANEQVVSDSAPGRI
                     YVGVSAPGKSLKTAYVRAAASLLSSAGGLRENPLLAEPYMTLVSYFNSLRELGGAIRL
                     MDDDIPARLEQLRSAGLPRRQRPVYVELTSRIRQEDIPLLLRRLERRHDAPRSEDDPL
                     PLDAVLASNMISVGVDIDRLGLMTVTGQPKTTAEYIQATSRVGRQVWGPGLVVTIYNW
                     SRPRDLSHYERFRHYHATLYRNVEAVSATPFSSRALDRGLRGTYVAMTRLGSDDWSPE
                     DGASRFAPQNPRVADVTEAFRRRADAVADASTAASLVQALSGLGDQWWGFRQNRIRYG
                     WRSPDPNRIPAEDVLLRVPEGGRIGHWPAPQSLREVEELSLVRILGIDGT"
BASE COUNT          557 a          758 c          802 g          612 t
ORIGIN      
        1 tacacactcc gcttgctcac gatacagcag ttccaacgag cagccacact gctctgtgcc
       61 tgcgaggttc ttagatctga tgatctggct acatggggcg actatcggtt ctgtataggg
      121 ctctggcttg gaatgcaggg aacgcccaac acacacgatg agtcacgtcg cgctctgctg
      181 cgtcttcagg aggacccctc ggaagaaggt ccgaaccctt gcgttcttga gtcgtgccca
      241 tggtgtggag aacgccttac gccgcaggag tactggccgg atggagaatc acgccgaacg
      301 cgggttgcct gtccgcgcac agaatgtgag ttccacaggt cacgaatgga gaatggaatt
      361 cccatcttag taatcgatga ggaaatttac cgagaatgtc cctcaatgct gattgcgacg
      421 gtcgacaagt tcgcacagat gccatggaac ggcgatgtgc aagcgctttt cggtttcgtc
      481 gatcgggaat gtgacaggtg cggcttcctc acgccggcga cggagcatgt caccgcgcac
      541 cggagctccg gaaagaagac tggcagctcg gcggtcagag aaagtgagcg tttagcaccg
      601 cccgatctaa tcatccaaga cgagcttcat ctgatctcgg gccctttggg gacactcgta
      661 gggctttatg aaacagccgt cgactttctg gctagccgtc gcgaacgtgg gaggctagtc
      721 gggccaaaag tggttgcatc gacggcaacc gtacggcgag cattcgagca ggtacaggcg
      781 gtgttccaaa gaaatttgag ggttttccca cctcccggac ttgagccgga agattccttt
      841 tttgcaaacg agcaggtcgt cagcgatagt gcccctgggc ggatttatgt tggggtttcc
      901 gcgccaggta agagtctgaa gaccgcgtat gttcgcgccg cagcgtcgct actctcctcg
      961 gccggcggcc ttcgagaaaa cccgctgctt gctgagccct atatgactct ggtttcctac
     1021 ttcaacagct tgcgtgagct cggtggcgcg atcaggctga tggatgatga tattccggcg
     1081 agacttgagc agttgaggtc ggctggtctc ccgcgacgtc agaggccggt atatgtggag
     1141 cttacaagtc gaatccgcca ggaggacatt cctcttttgc ttcgcaggct cgaacgacgg
     1201 catgatgctc ctcggtcgga ggatgaccct cttccgctag acgcagtgct cgctagcaat
     1261 atgatttctg tcggtgtcga tatcgatcga ctcgggctga tgaccgtcac tggccagccg
     1321 aaaacgacgg ccgaatacat tcaggctacc agccgcgtcg ggcgtcaggt ctggggacct
     1381 gggctcgtcg tgacgatcta caactggtca aggccacgag atctctctca ctatgagcgc
     1441 tttcgtcatt accatgcgac gctttatcgc aatgtggagg ccgttagcgc aacgccattc
     1501 tcatccagag ctctcgatcg tggccttcgc ggcacgtatg tagcgatgac tcgcctgggc
     1561 tccgacgatt ggtcgccgga ggacggagct tcacgatttg ccccccaaaa tcctcgcgtt
     1621 gcagatgtga ccgaggcatt caggaggaga gctgatgccg ttgcagacgc gtcaacagcc
     1681 gcttctctgg tgcaagcgct tagtggcctc ggcgatcagt ggtggggttt caggcagaac
     1741 cgcattcggt acggctggag atcgcccgat ccgaaccgaa tcccggctga ggacgtcctc
     1801 ctgcgagttc cggaaggagg acgaataggt cactggcctg cgccgcaatc gctccgagaa
     1861 gtagaggagc ttagtcttgt tcgcatttta ggtatcgatg gcacataaag cgggaacatc
     1921 cccagttttc ggagagctgc gtccgagtca gatcatcacg acctttggtc ctggatctgt
     1981 cgtggatctg cagaatgtct cggtgattgt tgcgggaact gatttctggg ttactcagcg
     2041 ggaccaagga atcgacgagc cgagactccg gagcatgctt cgagttcgtc atttgtatcg
     2101 accagcagtt cggtcggacg gcggagccgc cggcgtgcca tcattcatat tccctaagta
     2161 tctcttttgc ccgcgatgcc ggcgtctcgg acgatatgac cgttccgatc tcttttacct
     2221 tgaccgtcgt aaattccgtt gcaaggccaa acacgaggcc aaggttgcac ggggcggccc
     2281 agtcgcattt ccggcacgct tcgtcgtcgc gtgtactgag ggccaccttg acgactttcc
     2341 ctggtacgaa tacatccaca ggcgcaaagg tcacatcgcg tgcaaacctg agctactgcg
     2401 tttccgcgaa tccgccaaga gcgcggccat ctcggatctc gtagttagtt gtgatggctg
     2461 cgacgcatgg cgaacaatgg aggatgcttt ccctccccgt gccgccaatg cactgggccc
     2521 gtgtctcggt gcaagaccct ggctcggcga tcagaacact gaatcatgca caaagggagt
     2581 gttaaggacg atgctgcggg gtgcctcgaa tctctacttt gcccgtgtcc aaagtgcact
     2641 ttcgattccc gaatgggacg atcccatcca tttggcgatc gcctaccatg acgagcagct
     2701 tgcgaaggtt gactctatcg agaaactgc
//
LOCUS       DATDNW010000040         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_140742, whole genome shotgun sequence.
ACCESSION   DATDNW010000040 DATDNW010000000
VERSION     DATDNW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140742"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VI359_01040"
     CDS             <1..1258
                     /locus_tag="VI359_01040"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY6972864.1"
                     /translation="FLLRPKTLAAVKQNGLTIRSAAGSFTVRPRAASDPRDLPMPDLI
                     ILSVKAYDLEEVMAQLDPVMTDQTVILTLQNGVDTEDRLNARLKRDCVVGGVAFIYSK
                     IETPGVIDHYKKGSVAIGEMMGHKSPRLWAIAEIFKQAGIPCQLTDDVRRSKWEKMCW
                     NCVFNPLTVIIDDKVAKALDHPEMLRVIPQIVEEVAAVAAAVKVPLASDMAEKVLRWT
                     QEIRDIHTSMYDDWKAGRPTEIDYLNGYVAKLGRQFGIPTPLNEALTAMVKTITERDR
                     TGPGMLRIEGAVVQPVTFDHASISKLPPEHHVDVSTVMPNMQGKGIRLKGLLDVPALA
                     VQADHVTFHSGDGKYAASLSLQQANEFGILVYELDGQILPPEKGGPFRLITPGLGDLC
                     ANVKGVARVEVTKGVGADTRPSLRNC"
     gene            complement(1245..2597)
                     /locus_tag="VI359_01045"
     CDS             complement(1245..2597)
                     /locus_tag="VI359_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY6972865.1"
                     /translation="MGSLPRIRKSVLTLALPVTVSSLLQRAEGIVAVFLVGGLGATSI
                     AAVGLGQLLAFIATTLVSGLSVGANVLIAQLWGARRTRDGEDAATHLLVLGLVVSLVL
                     MAAGILFNRAAMILLGAHIDLINQAEPYSDLIFLVIPFTVLLHTLTSILQGTGDTRTP
                     MFVLIGVNLLHLALAYPLIYGLWGFPAWGITGAAVAVGVAEGAGFTYLLWACRHLFRI
                     PSTVRRDLLYATWRIGAPVSGERIFQQAGIMVYTKIVLLYGTVTYAAHQVGLSIESLS
                     FLPGYGFAIAAATMVGQSIGAGKYVRAKMENWEANRLAAGAMATMGVFFFFFPYVLLR
                     TFTNDASVIELGTTFLRIVALLQIPLALTMVLAGSLRGAGDTPFIMWATTIGMWGVRV
                     PWAIVAGSVLKLDILFVWSAMIADWTVRMGLLLWRYRSERWRTIQVIGQTSKKRASSF
                     "
     gene            2755..>3105
                     /locus_tag="VI359_01050"
     CDS             2755..>3105
                     /locus_tag="VI359_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEY6972866.1"
                     /translation="MKRVYLEVHHPQLASAPDASTQAMLDMGTEIGERARSLFLRGVL
                     IEESHRQREAALARTLSVINDPSVPAIFEGAFEHDGVLVRVDILQRVQSDTAGRVAWR
                     LIEVKSSSRVKDVHL"
BASE COUNT          728 a          837 c          930 g          610 t
ORIGIN      
        1 gttcttactg cgaccaaaaa cgttagcagc tgtgaagcag aatgggctga cgattcgcag
       61 cgccgccgga tcgttcaccg tacgcccacg tgctgcgtct gatccgcggg atctgccgat
      121 gccggatctg atcatcctct ccgtcaaagc ctatgacctc gaggaggtga tggcgcaact
      181 cgatccggtc atgacagatc agacggtcat cctcaccttg caaaatggcg tggacaccga
      241 ggatcgtctg aatgcacgat tgaagcgcga ttgtgtcgtt ggcggggtgg cgttcatcta
      301 ttccaaaatt gagacgccgg gtgtcatcga tcactacaag aaaggttcgg tggcgatcgg
      361 tgaaatgatg ggacacaaga gccctcgtct ttgggccata gcggagatct tcaagcaggc
      421 cggcattcca tgccaactca cggacgacgt tcgtcgcagc aagtgggaga agatgtgttg
      481 gaactgcgtg ttcaatccgc tcacggtcat cattgacgat aaagtggcca aagcgctcga
      541 tcatccggag atgctgcgcg tcatccctca gattgtcgaa gaagtggcgg cggtggcggc
      601 agccgtgaag gttccgctgg catctgatat ggctgagaag gtccttcgct ggacccagga
      661 aattcgagac attcatacgt cgatgtacga tgattggaag gccggacgtc ccacggagat
      721 cgactatctc aacggctacg tcgcaaaact cgggcggcaa tttgggattc cgacgccgct
      781 caatgaagcg ttgacggcga tggtcaagac gattactgaa cgtgaccgga ccgggcccgg
      841 catgctacgg atcgagggag cggtggtgca gcctgtgacg tttgatcatg catcgatctc
      901 aaaacttccc ccggagcatc acgtggatgt gtcgaccgtc atgccaaata tgcaggggaa
      961 agggatcagg ctcaagggat tgctcgatgt accggcgctc gccgtccaag ccgaccatgt
     1021 aacgtttcac tccggagacg ggaagtatgc ggccagtctc tcgctgcaac aggcgaacga
     1081 atttggcatc ctggtctacg aactcgacgg acaaatcctc ccgcccgaga aaggcggccc
     1141 gtttcgtttg attactcccg gtcttggcga tctctgtgcg aatgtgaaag gtgtggcgcg
     1201 ggttgaggtc actaaaggag tgggagcaga cacgagacca tcactcagaa actgctagcg
     1261 cgtttcttgc ttgtctgtcc aatcacctga atcgttctcc agcgttccga tcgatagcgc
     1321 cacaataaca gccccatccg tactgtccaa tctgcaatca tggcgctcca gacgaagagg
     1381 atatccagtt tcagaacaga tccggccacg atggcccacg gaacgcgcac gccccacatc
     1441 ccgatggtgg tcgcccacat aatgaaggga gtgtcgccgg ctcctctgag agagccggcc
     1501 aggaccatcg tcagtgcgag cggaatttgc aggagcgcga caatgcgcag gaaggtcgtg
     1561 ccaagctcga tgacagatgc gtcgttagta aaagtgcgaa gcaggacata ggggaagaag
     1621 aaaaagaaca cccccatcgt cgccattgca ccggccgcta agcggttggc ttcccagttt
     1681 tccatctttg cgcgcacata ctttcccgca ccgatgctct gccccaccat ggtggcggcg
     1741 gcaatggcaa atccatatcc tggcagaaag gacaacgact cgatcgacaa gccgacctgg
     1801 tgcgccgcat aggtgaccgt gccatagaga aggacgatct tcgtgtagac catgatgccg
     1861 gcttgctgga agatgcgttc gcctgaaacc ggggcgccga ttcgccaggt tgcataaagg
     1921 aggtcccggc gaacagtgga gggaatacga aacaaatgac gacaagccca cagcagatat
     1981 gtgaaaccgg caccttctgc gactcctacg gctaccgctg ctcctgtgat gccccaggca
     2041 ggaaagcccc agagaccgta gattaagggg taggcgagag cgagatgaag gaggttgacg
     2101 ccgatcaaga caaacattgg tgtccgggta tcaccggtgc cttgcaagat cgaggtgagc
     2161 gtgtgaagta agacggtgaa ggggatgacg agaaaaatga gatcggagta gggctcggct
     2221 tggttaatga ggtcgatgtg cgcccccaac aggatcatgg ccgcacggtt gaacagaata
     2281 ccagctgcca tcaggaccag agacacgacg aggcccaaga ccagcagatg tgtcgcggcg
     2341 tcttcaccgt cgcgggtgcg tcgagcgccc cagagttggg caatgaggac attggctccc
     2401 acggacagac cggaaaccag cgtcgtggcg atgaatgcca gcagctgtcc caatcccacg
     2461 gcggcaatcg aggtggctcc caaccctccg acgagaaaga cggcaacaat tccctcggca
     2521 cgctgcagca gactgctgac cgtgacgggc agggccaatg tgagcacaga ttttcgaatc
     2581 cgaggaagag atcccataga gcttatccta gcagaagcga gacctttcct tggtggacaa
     2641 atgggggatg ggttcgatag ggtctcttct gcaatgatgg aacccggcgt acctcgttct
     2701 gctgcgatcg cccttcgttt gtccaaatca aaatttttgg ccggattgca gtgtatgaag
     2761 cgtgtgtatc tcgaagtgca tcatcctcag ttagccagtg caccggatgc gtcgacgcaa
     2821 gccatgctcg acatgggaac cgagatcggt gaacgtgccc gaagtctgtt tctgagaggt
     2881 gtgctcatcg aagaaagcca ccgtcagcgg gaggcggcgc tcgcccgtac tctctcggtc
     2941 atcaatgacc cctcggtccc ggcaattttt gagggagcct tcgagcacga cggagtattg
     3001 gttcgcgtgg atattctcca gcgcgtccag agtgacacgg cagggcgggt ggcctggcga
     3061 ttgatcgagg tgaaatcctc ttctcgagtc aaggacgttc acctt
//
LOCUS       DATDNW010000041         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_550584, whole genome shotgun sequence.
ACCESSION   DATDNW010000041 DATDNW010000000
VERSION     DATDNW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550584"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VI359_01055"
     CDS             complement(<1..512)
                     /locus_tag="VI359_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6972867.1"
                     /translation="MGILIVDDSADDRLLVQSILSAAGYDHILVADSATSAFRLLGME
                     GGAQGTSRIDLILMDIIMPEMNGIEACRQIKAVERFRDTPIMMVTVKTDPVDLQLAFA
                     AGALDYIGKPINKIELLTRVRSVLRLVHEIDRRRAREQELLDVMRQLQEANQMLLRLS
                     CLDGLTGITNR"
     gene            complement(562..1200)
                     /locus_tag="VI359_01060"
     CDS             complement(562..1200)
                     /locus_tag="VI359_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6972868.1"
                     /translation="MSILIVDDSPDQQALLRSILGKAGHSDILTVDSAKSAFVTLKLE
                     DQVSLPSVDLILMDVLMPEQDGVEACREIKRRDHLRDIPIIMVTAKNDLSNLQAAFAA
                     GAVDYITKPVNNLELLARVSSALTLKQEMDCRKARELELRRSNEALQKALKEVKVLRG
                     LIPICASCKKIRNDSGFWQQLEEYLGEHSEAEFSHGLCQPCIKKLYPGVYHD"
     gene            complement(1315..1761)
                     /locus_tag="VI359_01065"
     CDS             complement(1315..1761)
                     /locus_tag="VI359_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY6972869.1"
                     /translation="MSLRDRLTEDLKQAMKSRDQLRMDVIRMIKASVLNKEVELKKDL
                     DDAEMSRVMTTMIKQRKESVEQFEKGNRAELAAKERQEIAIIQAYLPQALSPDQLAGV
                     IEAVIRDTGATSVKDMGAVMKAVMARLAGQAVDGKLVSDLVRSKLQ"
     gene            1956..2594
                     /locus_tag="VI359_01070"
     CDS             1956..2594
                     /locus_tag="VI359_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972870.1"
                     /translation="MVRRGSAVNVGRAAMMALMFAGCSSTPPPAIAGVELSFPVVAIP
                     SDVQRLAVLYPRGANSEWSSAYSRLEGAAFQLKAYRPTLRIIDRSNMPTVFTEQRFQT
                     GGLVSDESAVRIGQMLGVDSVLIYRIDGPTLRDRFWARQHQDLPPITVTSKIIRVESA
                     EVVYLNVVTARVDEADGSRWSLSDNVDYQRWNREALDRGIMQTVVNLQRAFQ"
     gene            2630..2842
                     /locus_tag="VI359_01075"
     CDS             2630..2842
                     /locus_tag="VI359_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972871.1"
                     /translation="MNHGRSSVQKVTRATVLGLIGCLGITACAGDTPKGPPPPTPDQV
                     RGHADRTFDKLKQEEQERGSQANTPR"
     gene            complement(2934..4262)
                     /locus_tag="VI359_01080"
     CDS             complement(2934..4262)
                     /locus_tag="VI359_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6972872.1"
                     /translation="MARGRRYGLRDGLCQAVTQGAGEQYLSAFALLLQTSPFQLGILS
                     ALPQLIGTWAQLLSVKVLRWFPDRKTLILAGTMGQAVVWLPIVLLPWLFPQWGPWLVI
                     AGAAGYFACNHFTTPAWNSFITDLLDPNQRGAYFAHRARIMAVISLLTLCIGGSLLTL
                     WQQYELAYIGFLTLFLIAGGARLVSARHLTRVQDPHHRAQIQDQDGFRRFLRKRATTD
                     FRWFVCFSGLMHVAVLIAGPFFVIYMLRDLRFSYWEYGGWLAAGILGQFLTLNRWGHF
                     GDRFGNKALLTATSFMVPFLPMGYLFSTKWAFLATLNFLGGVTWAGLSLGLQNYVFDT
                     VRPEDRAKAVALSNTINALGWSLGALTGSWLIHVLPGEIHIGWLTVAPESNLPLVFFL
                     SGCLRLLVSATLLGTFQEVRIVEQVPQHRLMWELPLLRSIAQFGVWKADR"
     gene            4474..>4859
                     /locus_tag="VI359_01085"
     CDS             4474..>4859
                     /locus_tag="VI359_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972873.1"
                     /translation="MNLFGLHAPAVSRVIAAVLSVSVSAGFLGGQSIARDLVTPKEQT
                     TTEFEPTQSPSFDEQKRGIPQSLFTWIKMAKGYEVEWDSFGRKGWYTQDPRLKPIEPT
                     TTFSPETPAIYIVFEVAPLEDPAQFSA"
BASE COUNT         1132 a         1289 c         1406 g         1032 t
ORIGIN      
        1 cgattggtga tgccggtcaa tccgtcaagg caggagaggc gcagcagcat ttggttcgct
       61 tcttggagtt ggcgcatgac gtcgagaagt tcctgctcgc gcgctcttcg ccgatcgatt
      121 tcatgcacta atcgaaggac cgaacggaca cgcgtcaaga gttcaatctt gttgatgggc
      181 ttgccgatat agtcgagtgc cccggccgca aatgcgagtt gcaaatcaac cgggtccgtc
      241 ttgacggtca ccatcatgat gggggtatcg cggaatcgtt ccaccgcctt gatttggcgg
      301 caggcttcga tgccattcat ctccggcatg atgatgtcca tgagaataag atcgatccgg
      361 ctggtgccct gggcgccgcc ttccatcccc agtagtcgaa aggcggatgt ggctgaatct
      421 gcgactaaaa tatggtcgta gccagcggct gagaggatcg attgcaccag caaccgatca
      481 tctgctgagt cgtcgacgat tagaataccc atcgcgtcag aatcctgccc agtagatggt
      541 gcgtgtgaga aagactagca gctagtcgtg gtaaacacca ggataaagct ttttaataca
      601 gggctgacag agtccgtgac tgaactctgc ctcggaatgc tcgccgaggt attcctccaa
      661 ttgttgccag aacccgctgt cattcctgat ttttttgcac gatgcacaga tagggatgag
      721 accgcgaagc actttcactt ccttgagagc cttctgaagg gcctcattgc tgcgacgcaa
      781 ctccaactcg cgcgctttcc ggcaatccat ctcctgtttg agcgtcaacg ccgacgagac
      841 gcgggctaag agttcaaggt tgttgacagg tttggtgatg taatcgacgg ctccagctgc
      901 aaaggcggcc tggagattcg agaggtcgtt tttcgcggtg accatgataa tcgggatgtc
      961 ccgtagatga tcgcgacgct taatctctcg acaagcctcc acaccatcct gttccggcat
     1021 cagcacatcc atgagaatga gatcgaccga cggtagggaa acctggtcct ccagctttaa
     1081 ggtgacgaac gcggattttg ctgaatccac ggtcaggata tccgaatggc cggcctttcc
     1141 gagaatcgag cgaaggaggg cttgttgatc tggagagtcg tcgacaatga ggatgctcat
     1201 acgcgcgtcc tacccgcggg cacaccgagt gactacgtag atgccttcat aggcacgtgt
     1261 tgtcagtata gcagggaaaa tagcttaggg aagggaaacc agaagttcgc aaatctattg
     1321 cagcttggat cggacgagat cgctgaccag cttgccgtcc acagcttgac ctgccaagcg
     1381 cgccatgacg gctttcatca ccgctcccat gtccttgacg gaggttgccc cggtgtcacg
     1441 gatgacggct tcaatcactc cggcgagctg atcgggagac agagcttgtg ggagataagc
     1501 ctggatgatg gcgatctcct ggcgctcttt tgcggccagc tccgcgcggt tacctttttc
     1561 aaactgttcg accgactctt tgcgttgctt gatcatcgtt gtcatcacgc gactcatttc
     1621 ggcgtcgtca agatccttct tgagttcgac ttccttattg agcacactag ctttgatcat
     1681 gcggatgacg tccatacgca gttgatcgcg tgatttcatg gcctgtttga gatcttcggt
     1741 caagcgatcg cggagtgaca ttctcacctc tcaggaaaag tcgcgtctgg actgtgcgga
     1801 gaataccgcg tcattggacc tcagtcaatg aggctgggtt atgagcatct agtgtctgtt
     1861 tgggatagtg gttttcaccc tgttgcggga gtatgattta gacaaagcct aggtgcaggc
     1921 tcgcataatc gaggcgaacg ttgggagtgc tggccatggt acggcgaggc tccgcagtca
     1981 acgtggggag agccgcgatg atggctctca tgttcgcagg atgctctagt actcctcctc
     2041 cagcgatcgc aggggtggag ttatcttttc ccgtcgttgc aatcccatcc gacgtgcagc
     2101 gcctggccgt gttgtatcca cgaggagcca attccgaatg gtccagcgcc tacagtcggc
     2161 tggaaggggc tgcgtttcaa ctcaaagcgt accgccccac gctcagaatt attgatcgtt
     2221 cgaacatgcc gaccgtcttc accgagcagc gatttcagac cggtggcctt gtctccgatg
     2281 agagcgccgt ccgaatcggc cagatgttgg gagtggacag tgtgctgatc tatcgaatcg
     2341 atgggccgac gctacgggat cggttttggg ctcggcaaca ccaggattta cctccaatta
     2401 ctgtgacgag caagatcatc cgtgtcgaaa gcgcagaagt ggtgtatctg aatgtggtca
     2461 cggctcgagt cgatgaggcc gatggctcac gatggtcgct ttccgacaat gtggactatc
     2521 agcgatggaa tcgggaggcg ttggaccggg ggatcatgca gacggttgtc aaccttcagc
     2581 gtgcgtttca ataatcagcg acaaactgat ccgaaaggag tgaaaggtga tgaaccatgg
     2641 acgaagcagt gtgcagaaag tcacgagggc aacggtgctc ggcctcatcg ggtgcctggg
     2701 tataacggct tgtgccggcg acacgccgaa gggcccacct cctcctacgc cggatcaggt
     2761 gcggggtcat gcggaccgga ccttcgataa actcaagcag gaggaacagg aacgaggctc
     2821 acaggccaat acgccccgct gaactcggag cacgcagtca acgtcaccgc gagatgttca
     2881 aaaaagccgt ctgggacatg acaacgaagc cgaagaacgt ttttagcatc ctgtcagcgg
     2941 tcggctttcc acacgccaaa ctgagcgatc gatcgcagga gcggcagttc ccacatgagg
     3001 cgatgttggg ggacctgctc gacaatcctg acttcctgga aggtcccaag caaggtcgcg
     3061 gacacgagca agcggaggca gccggagaga aaaaatacta acggcaggtt cgattcgggg
     3121 gcaactgtca accagccgat atgaatctct cccgggagga cgtgaattaa ccagcttccc
     3181 gtcaaggccc cgagtgacca tcccagagca ttgatcgtat tggacaatgc gaccgctttg
     3241 gcgcggtctt ctggccgaac ggtgtcgaac acatagtttt gcagccccaa cgaaagtccg
     3301 gcccaagtta cgccgccgag aaaattcagt gtggcgagaa aagcccactt tgtgctgaag
     3361 agatagccca ttggtagaaa ggggaccatg aaactggtgg cggtcaataa tgctttgttg
     3421 ccgaatcgat cgccgaaatg tccccaccgg ttaagggtta gaaattgtcc aagaataccg
     3481 gcggccagcc agcctccata ttcccaatag gagaagcgca gatcccgcaa catgtaaatc
     3541 acgaaaaaag gtcccgcgat caacacagcg acgtgcatga gaccggagaa gcacacaaac
     3601 caacggaagt cagtcgttgc ccgcttccgc aagaatcgac ggaacccgtc ttgatcctgg
     3661 atctgtgccc gatgatgcgg gtcctgcacc cgtgttaagt gccgtgctga cacgaggcgt
     3721 gcgccgccgg cgatcaagaa cagtgtgagg aacccgatgt atgcaagttc gtactgctgc
     3781 cagagggtca acaaggaccc tcctatgcac agggtcagaa gactgatcac ggccatgatt
     3841 cgagcgcgat gtgcaaagta tgctccgcgt tgatttgggt cgagcagatc ggtaatgaaa
     3901 ctattccatg cgggggtggt gaaatgattg caagcaaagt aaccggctgc tcctgctatg
     3961 acgagccacg gtccccattg cggaaacagc caaggaagca atacgatcgg gagccacacc
     4021 accgcttgcc ccatggttcc tgccaaaatc agcgttttgc gatcggggaa ccagcgcaag
     4081 accttcacag aaagcagctg cgcccacgtg ccgatgagct ggggcaaggc cgagaggatg
     4141 cccaattgga acggagaagt ctgcagtaac agggcgaagg cagacaggta ttgctccccg
     4201 gctccttgcg tgactgcctg gcaaaggcca tcgcggaggc catagcggcg acctcgcgcc
     4261 atgatgtcct ggtcggcctg agataggggc gaggaagctt ccaggcaaga tgctgtattg
     4321 gaactcacag ggtcggcttt ctgtactggt ccagcgtggt tgacttgctc tctgagccta
     4381 tcacagccag tggcagattg gggctggttc gaccggttat tgacccacgg gcaagcacca
     4441 gatacgatga aacgcagaag taagaggcga gcaatgaatc tttttggtct tcatgctccg
     4501 gcggtcagtc gagtcatcgc cgcggttttg tccgtttctg tcagtgcagg ttttctggga
     4561 gggcaaagca tcgcccgaga cctcgtgacg ccgaaggaac agaccacgac ggaattcgag
     4621 ccaactcaaa gtccgtcctt cgatgaacaa aaaaggggaa tcccccagtc tctgttcacc
     4681 tggatcaaga tggcaaaagg ctatgaagtc gaatgggaca gcttcggacg aaaaggatgg
     4741 tacacccaag acccgcgctt gaaaccgatc gaaccgacca cgaccttttc gcccgaaacc
     4801 ccggctatct acattgtatt tgaagtagcg ccgctcgaag acccggccca gttcagtgc
//
LOCUS       DATDNW010000042         7701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_550655, whole genome shotgun sequence.
ACCESSION   DATDNW010000042 DATDNW010000000
VERSION     DATDNW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550655"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1276)
                     /locus_tag="VI359_01090"
     CDS             complement(32..1276)
                     /locus_tag="VI359_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6972874.1"
                     /translation="MIDMSRRAFLQLSAAAGGALVFGELASQVLGVAPRSRPAYAAEP
                     IKIGIVDPLSSPYRTSSIHDVHGANVAVDLFNKRGGVLGRPVIILEADDASDPETAVK
                     AATKFIKEDRVDFLMGTFNGDCALAVSALAQKENKLFMVTGAHLPELTGVACNSHTFV
                     FMPNATMMSQAVVPHLVKAYGTRWYMITTSTLDGKSMAQAMATAGQAHGVEFVGEALM
                     PFGSTDFTSALAAAKDKHPSLVLLNLYGWDLIHALKTYAKLELAKEKIGVGGMIGGEQ
                     IGRPLGYANNVGIWGLIWDPKINTDSSRRFIQAVIDKYNHTPTSRCYLGYAAMTQILE
                     AVQRAGTTDTRAVIKALEGHEFDGLKEGKSVFRAWDHQHVQDVLVGEAFGKELGLGHY
                     KILATVPGETIAETIAQNTCKF"
     gene            complement(1491..2564)
                     /locus_tag="VI359_01095"
     CDS             complement(1491..2564)
                     /locus_tag="VI359_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6972875.1"
                     /translation="MRASISVLFILLVVLAGPVWAYRDYFTVEQKAQLAKIQTVLVDA
                     IALTDKGAIDAKPIAEVTSRRLQELGYTIIQDRTKPHDVLFKVKCEQRKTWEGTTTAG
                     GDADLPDAPSRLWKGPACQLNYVLGTMKINWQKEVRTDFEDAVAAAQAAKAADAGVYA
                     MDRLKERLEQYDFPVIVTAEWGQPDRLLKLLDKSDTSQLRKLKIISLLGEMEADEALP
                     KLKEALKDKELSKQATVALGNMGKEGIPILVDILKHSRQPELQAAAAKGLGDLGNIHN
                     DSTVIPPLLEMLDAPGIDITVQTEVAWALGRVPDRRSVKPLFELDKKLQKIRNDPPDP
                     QIKKLKEAVFWSIKQVYTEDQYS"
     gene            2996..3078
                     /locus_tag="VI359_01100"
     tRNA            2996..3078
                     /locus_tag="VI359_01100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3030..3032,aa:Leu,seq:tag)
     gene            3141..4451
                     /gene="tig"
                     /locus_tag="VI359_01105"
     CDS             3141..4451
                     /gene="tig"
                     /locus_tag="VI359_01105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY6972876.1"
                     /translation="MKMEVTELGPMKRALKIEVPADEVTQRFTRAYTELNRQVSIPGF
                     RPGKAPVALLEKKYAKTIEEDVIRSLVPDYYDRAVRQAGIVPVLVEIPPLERVKIKKD
                     TPFTFTATVEIKPKIDLRDYKAPNPISLKPDKRTVTDEQINRGLEVLREQHARLDASP
                     AGHAISDGDYVILDLEGSLDGKPLEGTKKEGQLHKIGSHAAILGIEIDRHVAGKHEGE
                     IVTIPQPYPASHPDQRVAGKTVTFVLTIKAVKQKMLPALDDEFAKDCGPYTSLREIKD
                     RLQSEMEKALKKEIEETYKDTILKRLVETHHFDLPETLVEREVEAIVRQQIQQQRRGG
                     ADPSTAGQENLKKLRDDNRQEAERRVKVGLILEAIAEKEGLSVTQDDVNAEIARLATE
                     LRMATAELVKMIQAGGRDSIDELRARILADKALDFVYRHAVIQG"
     gene            4492..5130
                     /gene="clpP"
                     /locus_tag="VI359_01110"
     CDS             4492..5130
                     /gene="clpP"
                     /locus_tag="VI359_01110"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY6972877.1"
                     /translation="MLVPIVIEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLVI
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGALL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LEKIAADTERDYFMSGEEAKRYGIIDEVITRPPKHLKAVSGGESGKDGAKDK"
     gene            5144..6400
                     /gene="clpX"
                     /locus_tag="VI359_01115"
     CDS             5144..6400
                     /gene="clpX"
                     /locus_tag="VI359_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY6972878.1"
                     /translation="MAKQEKMDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPSEIKHHLDQYAIGQERAKKILSVAVHNHYKRISSKEKE
                     VDDVELQKGNILMVGPTGTGKTLLAQTLAKYLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFICGGAFVGLEQIIEQRLNRKAMGFGAEVRGKSD
                     ARLGELFEKVQPEDFLKYGLIPEFIGRLPVVATLDELDERALIRILTEPRNALTKQYE
                     KLLSFEKVKLKFTDGALAAVARKAYCQKTGARGLRAILEEVMLDLMYDAPSQKQIKEV
                     VITEDAILGKHPPIRIFEQDKDAKSA"
     gene            6611..7129
                     /locus_tag="VI359_01120"
     CDS             6611..7129
                     /locus_tag="VI359_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6972879.1"
                     /translation="MKYPVASSLRHKGKTLDVDPVREVITLLGLNGEPLGSLSWDFVV
                     DQILAYRKPPVARETRAEPRVTLSFRVKYKTPEGHHFESRAGGIGGGGLFIESQSTLP
                     VGTKLVMEFSLPERPNEWLPAKGVVAWVCPKADQYTFSPGMGVRFTEIDPGVREQVLA
                     LVTSLQNMGQAV"
     gene            7140..>7701
                     /locus_tag="VI359_01125"
     CDS             7140..>7701
                     /locus_tag="VI359_01125"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6972880.1"
                     /translation="MPRQQDAAGALKGTDTMKFTVTATPGYEGHMLEMDVNQEAVLLR
                     GAKGQLIGTMPWSGVIDRILANDDDARFAHSRAHPRAPLAVKVRYMTPDGKQFDSLTG
                     GIGGGGLFIESSTPLAPGTELSVEFALPDRPWERLKAKAKVAWTRNKPERYLLFPGMG
                     VQFTDIDPGCQRQLVELVTALNRTRLT"
BASE COUNT         1830 a         2183 c         2092 g         1596 t
ORIGIN      
        1 ggagaagccc ccgtgtcatt ccagcaacct actagaactt acaagtattc tgcgcaatag
       61 tttccgcgat ggtttcaccc ggcacagtcg ccaggatctt atagtggccc aaacctaatt
      121 ctttaccaaa cgcctcgccg accaacacat cctgcacatg ctggtgatcc caggcccgga
      181 aaacggactt gccctctttg agcccgtcga attcgtggcc ttccaaagcc ttgatcactg
      241 cccgcgtgtc ggtcgtgccc gctcgctgaa cggcctcgag aatctgggtc atggccgcat
      301 agcccagata gcagcgtgag gtcggagtgt gattgtactt gtcgatcacc gcttggataa
      361 atcgacggga gctgtcggta ttgatcttgg gatcccagat caagccccag attcccacgt
      421 tgttggcgta cccaagcggc cgtccaatct gttctccccc gatcatccct ccgacaccga
      481 tcttctcttt cgccaactcc agcttggcat acgttttaag ggcgtggatc aaatcccacc
      541 cgtagagatt gagcaggacc agcgatggat gtttgtcttt cgcagccgcg agggccgatg
      601 taaagtcggt tgaaccgaac ggcatgagcg cctcgccgac gaactcgacg ccatgtgcct
      661 gcccggccgt tgccattgcc tgagccatcg acttgccatc gagtgtgctg gtcgtgatca
      721 tgtaccagcg ggtaccgtaa gcttttacca ggtgaggcac gacggcctgc gacatcatcg
      781 tagcgttcgg catgaacaca aaggtgtgcg aattacaggc cacgccggtg agctcaggaa
      841 gatgggcgcc ggtaaccatg aacagcttgt tttctttctg ggccaacgcg ctcacggcca
      901 acgcgcaatc gccgttgaac gtccccatca ggaagtccac acgatcctct ttgataaatt
      961 tcgtcgcggc tttcaccgcc gtctccggat cagaggcatc atccgcttca aggatgatga
     1021 ccggacgccc caatacccca cctcgtttat taaacagatc cactgccaca ttggcaccat
     1081 gcacgtcgtg aatcgacgaa gtcctatagg ggctcgacag aggatcgacg atcccgatct
     1141 tgatcggttc ggctgcgtac gccggtcgcg agcgtggcgc gacgccgaga acttggctgg
     1201 ccaattcccc gaagaccaag gccccgccag ctgctgccga gagttgcagg aacgctctgc
     1261 gagacatatc gatcatggtg acaatcctcc ccttttcaca gcagaccggc cagccaagcg
     1321 cccttggtta cactaaagtc aatcgctcgt gcaacagatt cttccactca gaagagagtg
     1381 cagggatgtc gagagcgcgc acaaggtctt caacccacga ccttacgctg gtgaggcatt
     1441 gtggaaggct tgctcgtctg agaacgatga tggaacttta cagcagcctg ttagctatat
     1501 tgatcttccg tataaacttg cttgatcgac cagaagactg cttccttcaa cttcttaatc
     1561 tggggatccg gcgggtcgtt ccgaattttt tgcaatttct tgtcaagctc gaacagcggc
     1621 ttcaccgacc gacggtctgg caccctgccc agagcccacg cgacctcggt ctgtaccgtg
     1681 atgtcgatgc cgggggcatc gagcatctcc agaagcggtg ggatgacggt cgagtcgttg
     1741 tgaatattgc ccaggtcgcc caatcccttc gcggccgccg cttgaagttc cggttgcctc
     1801 gagtgcttca ggatatcaac cagaatcggt attccttctt tccccatatt tccaagcgcg
     1861 acagtggcct gcttggacag ttctttatcc ttcagcgctt ctttcagttt cggcaaggcc
     1921 tcgtcagcct ccatttctcc caacagggaa atgatcttca attttcgcag ttggctcgtg
     1981 tccgacttat ccaatagctt gagtagacga tccggctggc cccactcggc cgtgacgatc
     2041 acggggaagt cgtactgctc cagccgctct ttcaatcggt ccatggcgta gacgccggca
     2101 tcggcggctt tcgcggcttg agccgctgcg accgcgtctt caaagtctgt tctgacttct
     2161 ttctgccaat tgatcttcat cgttccaagc acatagttca gctggcaagc cgggcctttc
     2221 cacagacggg acggcgcatc aggcaggtcg gcgtctccac cggccgttgt ggtgccttcc
     2281 catgttttgc gctgttcgca tttcacctta aacaggacgt cgtgcggctt agtgcggtcc
     2341 tgaataatcg tatagcccaa ttcctggaga cgtcgtgacg tcacctccgc aatgggcttg
     2401 gcatcgatcg ctcccttgtc tgtcagagca atcgcatcga ccagcactgt ttgaatcttt
     2461 gccagttgag ctttttgttc taccgtgaag taatcccgat aagcccatac aggcccggca
     2521 agcaccacca agagaataaa taggacggat atagaggctc gcatgaggca cctccttcgg
     2581 tagctgatcg aagaggagtg ggttgcttcc gcaacctcag gggctgagac ctgcggcccc
     2641 ccgtatctcc tgagcgcagg cacacgaagt gacggggcta tctaagtcag catattcatt
     2701 atagctgtca gcaaccgttc acgccacagg agtcttcctg aactcaggca tcggtgtctg
     2761 tgaggcacaa atcgccctgg agaagtcgag acagttcttc tgctgcacaa ggccttttca
     2821 ctcacaaatc gacggtgatt gtttcttctt cagcaccagg tttcactcag ccagagcccc
     2881 gcaggagtat gggttgacac cgggactctt ccaacggtac aaaaggttta catcatcgag
     2941 tattttcgcc tgtttgctga gctcattacg aggagtctcc ggtcctctcc cctgtgcgag
     3001 agtggcggaa ctggcagacg cgcgagactt aggatctcgt gggcaaccgt gggggttcaa
     3061 gtcccccctc tcgcaccaga acgatgacac cacagccggg tcgttttggc tgtgtcgctg
     3121 aagaagggtg tgtgcaattc atgaagatgg aggtcactga actgggaccg atgaagcggg
     3181 ctctcaagat cgaggtgcca gccgatgagg tcacacagcg gttcacaaga gcctacaccg
     3241 agctgaatcg gcaagtcagt atcccggggt tccgtcctgg caaagcaccg gtggctctgc
     3301 ttgagaagaa gtacgccaaa accatcgaag aggatgtgat ccggagcctg gttcctgact
     3361 attacgaccg ggctgtccgc caagccggta tcgtgccagt cttggtggaa atccccccgc
     3421 ttgagcgagt caaaataaaa aaagatactc ccttcacttt tacggcgacg gtcgagatca
     3481 agccgaaaat cgatcttcga gactacaaag caccaaatcc catttccttg aaaccggaca
     3541 agcggacagt caccgatgag cagatcaacc gaggcttgga ggtcctacgg gaacaacatg
     3601 ctcgcctcga tgcgtccccg gctggacatg cgatcagcga cggggactat gtcatcctcg
     3661 atctggaagg atctctggac ggcaagccgc ttgaaggaac gaaaaaggag ggtcaacttc
     3721 acaaaatcgg ttcgcatgcc gccatcctgg gaatcgagat cgaccgtcac gtggcaggaa
     3781 agcacgaagg tgaaatcgtc accatcccgc aaccctatcc cgctagtcat ccggaccagc
     3841 gggtcgccgg gaaaaccgtg acttttgtcc tgacgatcaa ggcggtcaaa cagaaaatgc
     3901 ttccggctct ggacgacgag ttcgcgaagg attgcggacc gtacacgtcg ctccgggaga
     3961 tcaaagaccg cctccagagt gaaatggaaa aggcgctgaa gaaggaaatc gaagaaacct
     4021 acaaagatac gattctgaaa cggctggtcg aaactcacca ctttgattta cccgaaacct
     4081 tggtcgagcg agaggtggaa gccatcgtcc gtcagcagat tcaacagcag cgacggggtg
     4141 gcgcggaccc ttcgacggct ggccaagaaa atcttaagaa actccgcgac gacaaccggc
     4201 aagaggccga gcgtcgcgtg aaggtcggac tgattctcga agccatcgct gaaaaagaag
     4261 ggctctccgt gacacaggac gacgtcaacg cggagattgc gagattggcg accgagctca
     4321 ggatggcaac ggccgagctc gtcaagatga ttcaagccgg agggcgggac tctatcgatg
     4381 aattacgcgc ccgcatcctt gcagacaagg cgctcgactt cgtgtatcgc cacgcggtga
     4441 ttcaaggata ggcacataac cgtactctgt cattcgaaag gaacgacgtg catgctagtt
     4501 cccatcgtca ttgagcaaac caaccgcggc gagcgcgcgt atgatatcta ctccagactg
     4561 ctgaaggacc gcatcatctt cctgggtgcg ccgatcgacg acgtctttgc gaatctggtc
     4621 atcgcccaac tgcttttcct ggaagcggag gatccggaaa aagacatcaa tctctacatc
     4681 aattctcccg gtggcagtgt cacggccggc ctgggcattt acgacacgat gcaatatgtg
     4741 aaaccgccca tcaacacgat ctgcctgggg caagcagcga gcatgggggc gttgttattg
     4801 acggccggca ccaaaggaaa acgctatgcg ttgcccaacg cgcgagtcat gatccaccaa
     4861 ccgatgggag gatttcaggg ccaagccacc gaaatcgaca ttcatgccag agaaattctc
     4921 aagatccgcg agcggctgaa cgaaatcatg gcgaaacata cgggacagcc gttggaaaag
     4981 attgcggccg ataccgagcg cgactatttt atgtcgggcg aagaagccaa acgctacggc
     5041 ataatcgacg aagtgattac gcgccctccc aagcacctca aagcggtgtc tggcggggag
     5101 agtggaaaag acggggccaa ggacaagtaa cacaggagga cgcatggcca agcaggagaa
     5161 gatggatcgg catctccgct gttcgttctg cgggaaaagc cgagacgaag tccgtaagct
     5221 catcgccggt cccactgtct atatctgcga tgagtgcgtc aatctgtgca acgacatcat
     5281 cgccgaggat tgggaagaag ccaaagaaga gatttcctcc aaactcaaga aaccttccga
     5341 aatcaagcac catctcgatc aatatgccat tggccaggag cgggcgaaga agatcctgtc
     5401 cgtggccgtc cataaccact acaagcgaat ctcctccaag gagaaagagg tcgacgacgt
     5461 cgagttgcag aagggcaaca ttctcatggt cggccccacc ggcacgggta aaaccttgtt
     5521 ggcgcagacc ctggccaaat atctagatgt gccgttcaca ctggccgatg ccaccacgct
     5581 gaccgaagcc ggatacgtcg gcgaagacgt cgaaaatatc atcctcaaat tgctgcaagc
     5641 cgcagactac gacgtcgagc gcgcggaacg cggtatcgtt tatatcgatg aaatcgacaa
     5701 gatcagccga aaaagcgaca gcccgtcaat aacgcgcgat gtctcgggtg aaggtgttca
     5761 gcaggcgctc ttgaaactga tcgagggcac ggtcgccaac gttccgcctc aaggggggcg
     5821 caagcatcct caccaggaat ttattcaagt caatacgagc aacatcctgt tcatctgcgg
     5881 cggcgcgttc gtcgggctcg agcagattat cgaacaacgg ctgaatcgca aagcgatggg
     5941 attcggcgcc gaggtacgtg gaaagagcga cgccagacta ggagagcttt tcgagaaagt
     6001 tcaaccggag gactttctca aatatggctt gatcccggaa tttatcggcc gtcttcctgt
     6061 cgttgccaca ctggacgagc tcgacgagcg ggcattaatt cgtatcctta cagaaccacg
     6121 caacgctctg accaaacagt atgaaaagct gctctccttt gaaaaggtca aactgaaatt
     6181 cacggatggg gccttggccg cagtcgcgcg caaagcctat tgtcaaaaga ccggtgcccg
     6241 gggactccgc gcgatcctcg aggaagtcat gttggacttg atgtacgatg ccccttcgca
     6301 gaaacagatc aaggaagtcg tcatcaccga agatgcgatt ctcgggaaac atccccctat
     6361 ccggatcttc gaacaggaca aggacgccaa gagcgcgtaa ctagttgaaa tcattttcaa
     6421 gtctcgccgt cgttgaacgt gcctcggcga ggtgacgttc gctgtgcccc tcccatttcc
     6481 caccctgata agacgagagc ctacgggcta ggtcttaagg caaagatcgc cccattacgc
     6541 gtctctggtt tttctcgaca tcgaagcaag ctgcgctata cttgcattgt tgcaagtagc
     6601 gggaggactt gtgaaatacc cggttgcttc gagtctacga cacaagggaa agacgctcga
     6661 cgttgatcct gtccgtgaag tgatcactct cctcgggctt aatggcgaac cactcggcag
     6721 cctttcgtgg gatttcgtcg ttgaccaaat cctcgcctac cgaaaaccgc ccgtcgcacg
     6781 ggagacccgc gccgaacccc gggttacctt atcatttcgt gtgaaataca agactccgga
     6841 gggtcatcac ttcgaaagtc gtgcgggtgg catcggcgga ggcggactct ttatcgaaag
     6901 ccagtcgact ctccccgttg ggaccaagct cgtcatggaa ttttcactcc ctgaacgccc
     6961 gaatgagtgg ctgccggcaa aaggcgttgt ggcttgggtc tgccctaaag ccgatcagta
     7021 cacgttcagc ccagggatgg gcgtccgatt taccgagatt gatcccgggg tgcgagagca
     7081 agtgttggcg ttagtcacat ctctccagaa catgggacaa gcagtttagg acacagcaca
     7141 tgcccagaca gcaagacgcc gccggagcgc tcaaagggac cgacacgatg aagtttactg
     7201 tgaccgcgac acccggatac gaaggccaca tgcttgaaat ggacgtgaac caagaagccg
     7261 tcttacttcg gggagccaag gggcagctga ttggaaccat gccgtggagt ggggtaatcg
     7321 accgtattct cgcgaacgat gacgatgcgc gatttgccca ttctcgagcc cacccacgag
     7381 cccccctggc tgtgaaagtt cgatatatga cccctgacgg caaacagttc gatagcctga
     7441 ctggcgggat cggcggaggc ggcttattca tcgagagcag cacccccctt gctccaggca
     7501 cagagttgtc agtggagttt gctctcccgg accgcccatg ggaaagactc aaggccaaag
     7561 caaaagtggc gtggaccaga aacaaaccag aacgctatct cctcttcccg ggaatgggag
     7621 tccaattcac agatattgac cccggttgcc agcggcaatt agtcgagctc gtcaccgcat
     7681 tgaaccggac tcggttgacg a
//
LOCUS       DATDNW010000043        10422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_141596, whole genome shotgun sequence.
ACCESSION   DATDNW010000043 DATDNW010000000
VERSION     DATDNW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141596"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="VI359_01130"
     CDS             <1..2069
                     /locus_tag="VI359_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY6972881.1"
                     /translation="PVIVIPAVKVNGVDFDLAKQDITVANVAIEQASWIIWLNPDGAV
                     NYQELFTPGTSSAATTPPAGASPLRKEEKPWTVLLKQISLKDHAVDFEDRSLQTPARV
                     KIASLTAETHDVHLPLKDPLPLSVEMQINETGQIRVTGSVAPSPFRADLTLGLKDIAI
                     RPFQPYFEKFARIDVLSGAVNVDGDLHLAVQHPRGPFLSYDGNANVTGLAIADLDQEN
                     EVASMKRFSLNTIHLTVDPTSLSIREVGLQEPAVHLVVQPDGGLNLAKLNSSEPSPAP
                     TDQKPPSAEKSKRAAVPVTIGTVKLLKAAATFRDETVQPPVDTGISDFTGTIKGLSSK
                     QLARADVDLAGLVDRVAPLKIVGTINPLSEDAFTDLVITFQNLDLTAEGPYSRKYVGY
                     ELSKGKLSFDLKYKVSQKSLEAENKILVDQLTFGEKTDSPDATSIPVPFVVALLQDRQ
                     GRIEIDLPIRGDLNDPDFKYGRVLLSTLGNILIKVAASPFSLLGKLVPGGGGGEELQY
                     FEFEPGSTSLAASELKKAEALIKALQERPGLRLEITGTADLVRDRQALRLQTLKTQLK
                     TKWQQERALPKDADFPDYEEERMIKERFEQEHVQPAGTVPAPKPDVAPKPPTIEEMRQ
                     HLASSIPVDEASLRSLADERAKQMRSQLAGEGKLADERVFLTEVDVTASGYEKVRSRL
                     NITAGS"
     gene            2165..2800
                     /locus_tag="VI359_01135"
     CDS             2165..2800
                     /locus_tag="VI359_01135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY6972882.1"
                     /translation="MALRLGDEAPNFTAETTEGTINFHEWLGNGWGILFSHPKDFTPV
                     CTTELGYMARIKDDFAKRGVKVIAISVDPLDSHRGWTKDINETQHCTVSYPLIADPDK
                     KVAMLYDMIHPNALDNMTVRSVFVIGPDKKVKLMLTYPASCGRNFDELLRVVDSLQLT
                     AKYKVATPVNWKDGQECIITPAVTDAEAKTLFPKGFKAVKPYLRYTPQPNK"
     gene            complement(2900..5086)
                     /locus_tag="VI359_01140"
     CDS             complement(2900..5086)
                     /locus_tag="VI359_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6972883.1"
                     /translation="MGIPTVLLLIPPLTQLNTPYPSTAYLTGFLRSRGIPCEQADLGI
                     EMVLRLFSRSGLTALFEEIRRSEKDLPGEARQMLSVEQAYLDAIDPVIEFLQGRNPSL
                     ATLLARPGFLLQGPRFHGRVRKQSAVRRSADRDRATQFATFFLEDLADLVQATVSPHF
                     ALSRYAEHIARAASSFETIIQALAPKPTLTDRFMLDALWTHLDRMNPSLVGLTVPFPG
                     NLYGAFRIAQSIKANRPDITIALGGGYANTELRRLTDPRVFDYVDYVTLDDGERPLLS
                     LIEHLAGTRPRAQLSRTFYRDNGRVVFANESADREFGMDEVGCPTYSGLALDRYLTIL
                     DSTNPMHRLWSEGHWNKLTVAHGCYWKQCTFCDVGLDYIGRFEMTPTDRLIYQIEQLL
                     AETGRRGFHFVDEAAPPAALKALALALLERGITISWWGNIRFEETFSPDLCRLLVASG
                     CIAVTAGLEAASDRLLETIKKGITVDQTALVAAAFREAGILIHAYLMYGCPSETVQET
                     VDSLERVRQLFAADLIQSAFWHRFTATAHSPIGLAPAAHGVRIVGPAFHGFAENDLVH
                     EDRVGKAPEWLGEGLRRSMLNYLEGRGLTLDVRRWFDHSVPRPRVPQAWVKRLLRGRM
                     VKDDPCIERRVVWLGGTPICTAIEKGTCVILPGRERDATLTLPKAEARWLTDLIRQAT
                     PQSKRSQPYPLLREAQSTFPGNTKEFATYLTSPSWKKVRDAGLLLV"
     gene            5363..6142
                     /locus_tag="VI359_01145"
     CDS             5363..6142
                     /locus_tag="VI359_01145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY6972884.1"
                     /translation="MSLKVIVISLIVLCGVSSAFAEIDEKKLLDLTYPLDERTVFWPT
                     NKPFTWEKAAWGKTPKGYWYASGDFSMSEHGGTHIDAPIHFGEGRLSVDAIPLQNLVA
                     PAVVMDVRVAVEQDWDYRLTVRDVEVWESRHGRIPAGAVVLMLTGWGKRWPDNHRYLG
                     SNTPSDPKTLHFPGYSKEAAEFLVTQRKVDGIGIDTPSIDYGPSQDFIVHQIVNGADR
                     YGLENIANLDQLPPKGATLVALPIKIKGGTGGPVRIMAILP"
     gene            complement(6199..6618)
                     /gene="msrB"
                     /locus_tag="VI359_01150"
     CDS             complement(6199..6618)
                     /gene="msrB"
                     /locus_tag="VI359_01150"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY6972885.1"
                     /translation="MGKQPEIGPIVKVIKTDEEWKKLLAPEAYRVLRHEETERPFVNP
                     MHENHQTGIYNCAGCDLPLFSSEHKFDSGTGWPSFWQPIDPKVIGTTTDFKLIFPRTE
                     VHCARCDGHQGHVFKDGPKPTGLRYCINGVALKFVPA"
     gene            complement(6708..9080)
                     /gene="lon"
                     /locus_tag="VI359_01155"
     CDS             complement(6708..9080)
                     /gene="lon"
                     /locus_tag="VI359_01155"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY6972886.1"
                     /translation="MSEQSFVSNLPVLPIKRTVLFPGVMMPLTVGRDRSIAAVEAAMR
                     TEDKTLLVIAQRAPDVDGPELKDLYSIGTKAVIKQIARVSEGQIHAMVQGLERVVLLR
                     LDQTDPYLVARVREYPEPTDTGTEVDALHRAIQDVVADLPKLIRAPGMEEAVVALGAE
                     QDPVALAYRIASLLNLTVQEEQKLLEATTRVDLLRSLYAALSREIQILQLREKITTEA
                     QAKISKSQREYILREQLKAIQQELGESDSEEGETGRLKKQIEETNLPDHVRKEAEREV
                     TRLAKIPPTSPDHQVIRSYLELVLELPWKKASEDSLELARVRQVLDEDHYGIKEVKER
                     IVEHLAVLKLNPGAKAPILCLVGPPGVGKTSLGQSIARAMGRKFERFSLGGVHDEAEL
                     RGHRRTYVGALPGRIIQAVRRAGVNNPVLMLDEVDKMGRDFRGDPAAALLEVLDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEIIQLGGYSEREKAEIAMRYL
                     WPRRLNEAGLQPSEVILAPEVLDRIITRYTREAGVRQLEQMLGRLTRKVALQFAEVTE
                     GGERAPVAVAMDQLPEWLGRERFIPEEARKELPPGVATGLAWTPTGGDVLYIETSLLP
                     GSHELTLTGQLGEVMQESARAARSYLWAHAEEMGLDISRFKRNGVHIHVPSGAIPKDG
                     PSAGITMATALASAYVRKPIRKDTAMTGEVSLSGLVLPVGGIKEKVLAAHRTGIKRII
                     LPKANEKDLKEVPEEVRRDIEFIPVETVHEVLRAAFTTVERHEEPCQVSA"
     gene            complement(9259..>10422)
                     /locus_tag="VI359_01160"
     CDS             complement(9259..>10422)
                     /locus_tag="VI359_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972887.1"
                     /translation="YELDGRFGLVIDLGGNDLYRGTIAASASEDQGNAVVIDLSGNDT
                     YDGTPLGLATGRLGVGLLIDHAGDDVYQLERGSGGAGFGGLGILFDGKGNDAYMGNRL
                     TQGAAIGGMGLLLDSAGNDRYTSYGFAVGFGGPLGVGALVDVTGDDHYQCGDKYPSAY
                     NAQDAPNGKPGDPLFQYDCFGLGTGSGQRILTKRLEWQAHNLAGGWGVFLDIDGHDHY
                     QSANFSQGHGYFFGTGLKLDLDGDDDHQAARYGHGASAHFGAALFIDRHGEDHYGSSG
                     PLYNAGVAWDHSAGLMIDGGKGRDTYLFDHSTGLGMADLTSWSVFIEEGGADQYRVSR
                     GMGAADTTSLGAFFDLAGTDDYPDLPTTNSSGTLKRTNNTIQQDTAGGLFVDK"
BASE COUNT         2180 a         2918 c         3040 g         2284 t
ORIGIN      
        1 atccggtgat tgtcattccg gcggtgaagg tcaatggtgt ggatttcgat ctcgcgaagc
       61 aagacatcac ggtcgcgaac gtggcgatcg agcaggcttc atggatcata tggctgaatc
      121 cagacggggc agtcaattac caggagttat ttacgccggg cacctcttca gcagccacca
      181 ctcctcccgc cggtgcgtcg cctttacgca aggaagagaa gccctggacg gttctgctca
      241 aacagatctc actcaaggat catgcagttg acttcgaaga tcggtcactt cagacccctg
      301 cacgcgtcaa gattgcttct ctcacagcgg agacccatga cgtccacctt cccctgaagg
      361 acccccttcc tctgtccgtc gagatgcaga tcaatgagac gggccagatc cgcgttacgg
      421 gctcggtggc gccaagccct tttcgggcgg atctgaccct gggcttgaaa gacatcgcga
      481 tcaggccgtt tcaaccttac ttcgagaaat tcgcacgaat cgatgtcctc tccggagcgg
      541 tgaatgtgga tggtgatctg cacctcgcag ttcagcatcc tcggggtccg ttcttgtcct
      601 acgacgggaa tgccaatgtg acgggactgg ccatcgccga tctggatcag gagaatgaag
      661 tggcttcgat gaaaaggttc tctctcaata caatccatct gaccgtcgac ccgacctccc
      721 tatcgattcg agaggttggt ctccaagaac cggccgtgca tcttgttgta cagccggatg
      781 gaggattgaa tcttgcgaaa ctgaattcgt ctgagccctc gccagcgccg accgaccaga
      841 agcctccatc ggccgagaaa tccaagcgcg ctgctgttcc cgtgacgatc ggcacggtta
      901 aattgttgaa ggctgctgcc acgtttcgtg atgaaacggt tcagcctccg gtggacacgg
      961 gaatctcgga cttcaccgga acgatcaagg gattatcctc caaacagttg gcgcgggccg
     1021 acgttgatct ggccggcttg gtcgatcggg tcgcgccgct gaagattgtc gggaccatca
     1081 atccgctgag cgaagacgcc ttcaccgatt tggtcatcac attccaaaat ctggacctga
     1141 ccgcagaggg gccctacagc cgaaagtatg tcgggtacga attgtcgaag ggaaaactct
     1201 cgttcgattt gaaatacaag gtctctcaga agtcactcga agcggaaaac aaaatcctcg
     1261 tcgaccaact gacgttcggt gagaagaccg acagcccgga tgcgacgtcg attccggttc
     1321 cattcgtcgt ggctctcctc caggaccggc aggggcggat cgagatcgat ctgccgattc
     1381 gcggcgatct caatgatccg gacttcaaat acggaagggt tctgctctca actctgggca
     1441 atattctcat caaggtcgcc gcctctccgt tttctttgct ggggaaactt gttcccggcg
     1501 gtggtggcgg tgaagagctg cagtacttcg aattcgaacc agggtcgacg agtttggcgg
     1561 catcggagct gaagaaagcc gaggccttga tcaaggcgct tcaggaacga cctggtttgc
     1621 ggttggaaat taccggaact gcggaccttg tgcgtgatcg ccaagcgctc cgcctgcaga
     1681 cgctcaagac ccaactgaag actaagtggc aacaggaacg ggcgctaccg aaggacgcag
     1741 acttccctga ttacgaggaa gaacggatga tcaaggagcg gttcgagcag gagcatgtcc
     1801 agccggccgg cacggtcccg gccccgaagc cggatgtggc tccgaagccg ccaacgatcg
     1861 aggagatgag gcaacatctg gcttcgtcca ttcccgtcga tgaggcaagc ctccgcagcc
     1921 tggcggatga gcgggcgaaa cagatgcgca gccaattagc cggcgaaggc aagcttgccg
     1981 acgaacgggt ctttctcacg gaggttgatg tgaccgcgtc aggctatgag aaggttcgga
     2041 gccgactgaa tatcacagct gggtcataac tgcagactct ccccttgaac ttggtgtgat
     2101 agagacagta tcgtagagcc attcatctgt tagccgggca gatccgtaaa caaggaggtc
     2161 gatcatggca ctacgattgg gagacgaagc accgaatttc acggcggaga caacggaagg
     2221 gaccatcaac tttcatgaat ggttggggaa cggatggggg attctctttt cacacccgaa
     2281 ggacttcacc ccggtatgca cgacggaact gggttatatg gcgaggatca aggacgactt
     2341 cgccaagcgc ggggtcaagg ttatcgccat cagcgtcgac cccctggatt cccatcgcgg
     2401 atggaccaag gatatcaatg aaacgcagca ctgtaccgtc agctacccgt tgattgcaga
     2461 tccggacaag aaggtggcga tgctgtacga catgattcac cctaatgcgc tggataatat
     2521 gaccgtcagg tccgtgtttg tgatcggacc cgataaaaag gtcaagttaa tgctcaccta
     2581 tccagcttca tgtggccgga actttgacga attattgcgc gtcgttgatt cgcttcaact
     2641 cacagccaag tataaggtgg cgacaccggt gaattggaag gatgggcagg agtgcattat
     2701 cacaccggcg gtaaccgatg ccgaagcgaa gacattgttc ccgaaaggat tcaaagccgt
     2761 aaagccgtac ctccggtata cgccgcagcc gaataaatag cctgtcgcgt gattctaaca
     2821 agggcgggag tggcgatgcg ccattcccgc ccttgtagtt tgtaggaagg tggtgctgcc
     2881 ggttgttatt ccgactgact tacacaagca agaggccggc gtcgcgaact tttttccagg
     2941 acgggctggt taggtaggtt gcgaactctt tggtgttccc tggaaaagta gactgagctt
     3001 cccggagcag gggataaggt tgtgaccgct tactctgtgg agtggcctgc cgaatcagat
     3061 ccgtcagcca tcgcgcttcc gccttgggca gagtcagagt ggcatcgcgc tctcgtcctg
     3121 gcaggatcac acacgtccct ttctcaatcg cggtacaaat cggtgtccct cccagccaga
     3181 ccactcgtcg ttcgatacac gggtcatctt tgaccatcct gcctcggagg agacgtttca
     3241 cccaggcctg cggaacgcgt ggtcgtggaa ctgagtggtc gaaccatcgt ctaacatcga
     3301 gagtcaggcc gcgcccttcg aggtagttca gcatcgagcg gcgtaaacct tcgccgagcc
     3361 attcgggagc cttgccgact cgatcttcgt ggaccaaatc gttttcggca aatccgtgaa
     3421 atgcgggacc gacaatccgc acaccgtgtg cagcgggcgc gagcccgatc ggactgtggg
     3481 ccgtcgcggt gaaccggtgc caaaaggcgg attgaatgag gtctgcggcg aacaactggc
     3541 gaacccgctc gagcgaatcc accgtttctt gaactgtttc agagggacat ccatacatca
     3601 gataagcgtg gatcagaatg ccggcttctc tgaatgccgc tgccacgagg gcggtctgat
     3661 ccacggtgat gcctttcttg atcgtttcca gcaatcggtc ggacgctgct tcgagtccgg
     3721 cggtgaccgc gatgcagccg gaggcaacca agaggcggca caagtccggt gagaaggttt
     3781 cctcgaatcg gatatttccc caccacgaaa tggtgatgcc tcgttccagc aatgcaagcg
     3841 cgagggcttt caatgcagca ggcggtgccg cttcgtcgac gaagtggaac ccgcggcggc
     3901 cagtttcggc gagcaattgc tcgatctggt aaatcagccg atcggtcggg gtcatctcga
     3961 accggccgat gtagtccaag cccacatcgc aaaacgtgca ttgcttccag tagcagccat
     4021 gagcgacggt cagtttgttc caatggcctt cggaccacag ccgatgcatc gggttcgtgc
     4081 tgtctaaaat cgtcaggtac cgatcgaggg cgaggccgct ataggtcggg caaccaactt
     4141 catccatgcc gaactctcga tccgcacttt cattggcaaa cacgactcgg ccattgtctc
     4201 gataaaacgt gcgggagagt tgcgcgcggg gcctagttcc tgccaggtgt tcgatcaagg
     4261 acagcagcgg ccgctctcca tcgtcgagcg tcacgtaatc tacatagtcg aacacccggg
     4321 ggtcggtgag acgtctcagt tctgtgttcg catagccgcc tcccagtgca atcgtgatgt
     4381 caggccggtt cgccttgatc gactgagcga tacgaaaggc tccgtagaga ttgccgggaa
     4441 acggcacggt caaaccgacg agagaaggat tcatacgatc gagatgggtc cacaaggcgt
     4501 cgagcatgaa tcggtctgtg agcgtcggct tgggggccaa tgcctggatg atcgtctcga
     4561 acgacgaggc agcgcgcgcg atgtgctccg catagcggct caaagcgaag tgaggcgata
     4621 cagtcgcctg aaccaggtcg gccaggtctt cgaggaagaa cgtcgcgaat tgcgtggcgc
     4681 gatcacggtc tgctgagcgt cgcactgcag actgcttcct gacgcgtcca tgaaaccgtg
     4741 ggccttgaag aaggaatcct ggacgggcga ggagagtcgc caacgaggga ttgcgtcctt
     4801 gcagaaattc gatgaccgga tcaatggcat ccagataggc ctgttcgact gagagcattt
     4861 gtcgggcttc ccccgggaga tccttctcgc tgcggcgtat ctcctcgaac agggcagtga
     4921 gtccggatcg actgaacaat cggaggacca tctcaatgcc gaggtcagcc tgctcgcacg
     4981 ggatgccgcg ggagcggaga aaaccggtga gatacgccgt cgacgggtag ggggtgttca
     5041 gttgcgtcag cgggggaatc agcagtagaa cggttgggat gcccatgagc tcacatacca
     5101 tagtttgagc atgttgcaaa agccctccag cgtcgttctc gcatcgctca aggcctcaac
     5161 gtacgtgacg gtgtacgcct cggcctttca ctcgctgcgg ccttgctggg cgggcttttt
     5221 cagcatgctc tgtgataagc atggtttcca cgcgcacttc aggtaagaat tcgcttgtcc
     5281 ggcatgttcg gggtacaaca gtctaagttt atggaaccaa ggccagctga ggccgagtca
     5341 ttagaggacg atggaggctg atatgtcgtt gaaggtgatc gtgatcagtt tgatcgtgtt
     5401 gtgtggcgta tcatcggcct tcgcggagat cgatgagaag aaactcctcg atctgaccta
     5461 tccgttggat gaacggacgg tattctggcc cacgaacaag cccttcacgt gggaaaaggc
     5521 cgcgtgggga aagacgccca aaggttactg gtatgcttcc ggtgatttct ccatgtcgga
     5581 acatggcggg acccatattg acgcgccgat tcattttggc gaagggcggc tgtcggtcga
     5641 tgccatcccg cttcagaatt tggtggctcc ggcagtggtg atggacgttc gcgtggcggt
     5701 tgagcaggat tgggattatc gattgacggt ccgtgatgta gaggtctggg aatcacggca
     5761 tgggcgcatt ccagccgggg cggtggtcct gatgctcaca ggttggggta agagatggcc
     5821 ggataaccat cgctatttgg gaagcaatac accgtcggat cccaagacgt tgcatttccc
     5881 gggatattcg aaagaggcag cagagtttct cgtgactcaa cggaaagttg acggcatcgg
     5941 catcgacacg cccagtatcg attacggccc ctcgcaagat ttcatcgttc accagatcgt
     6001 gaatggtgcc gacaggtacg gtctggagaa cattgcgaat ctggaccagc tcccgcccaa
     6061 aggtgcaacc ctcgtggcgt tgcctatcaa gattaaggga ggcacaggcg gacctgtcag
     6121 aattatggcc atcctgccgt gatgatggct gtttgaatcg cagaagcaaa cgtagccgag
     6181 gctcaaagtg ctcagtggct aggccggcac gaacttcagc gcgaccccgt tgatgcagta
     6241 gcgcaggcct gtaggtttgg gaccgtcctt gaagacgtgg ccttggtggc cgtcgcagcg
     6301 ggcgcagtgg acttccgtcc gtgggaaaat cagtttgaag tcggtcgtcg taccgatcac
     6361 cttgggatcg atcggctgcc agaaactcgg ccagcctgtg ccgctgtcga acttgtgctc
     6421 ggacgaaaag agcggcaggt cgcacccggc acaattgtag atgcccgtct gatggttttc
     6481 gtgcatgggg ttcacgaaag gccgctcggt ttcctcgtgc cgcaggacgc gataggcctc
     6541 tggtgccagg agcttcttcc actcctcgtc ggtcttgatg actttgacga tcgggccgat
     6601 ttccggttgt ttgcccatcg ggaggctcct ttcttccatg gtgttctcgg tcgggtcatc
     6661 gccgcgaacc ctcgaggcga tgacctctcg ttatacttcg tcggttttca ggcggaaacc
     6721 tgacaaggtt cttcgtggcg ctccaccgtg gtgaaggctg ctcgcagtac ctcgtggacg
     6781 gtctcgaccg ggatgaactc gatgtcacgc cggacctcct ccggaacttc cttgaggtcc
     6841 ttctcgttgg ccttcggcag gatgatccgc ttgattcctg ttcgatgcgc agcgaggact
     6901 ttttctttga tcccgccgac cggaaggacc aatccactga gactcacctc tcctgtcata
     6961 gccgtgtctt tccggatcgg tttcctgacg taggccgatg ccagcgccgt ggccatcgtg
     7021 atgccggcgg acggaccgtc tttcggaatc gctcccgacg gcacatggat gtgcaccccg
     7081 ttccgcttga agcgggagat atcgagcccc atttcctcgg catgagccca gagataactg
     7141 cgggccgcgc gcgccgattc ctgcatgacc tccccgagct gcccggtcaa ggtgagctcg
     7201 tgactgcccg gcaataaact ggtttcaatg tacaggacgt cgccaccggt cggggtccag
     7261 gcgagcccgg tcgccactcc cggagggagt tcctttctgg cctcctcggg tatgaatcgt
     7321 tctctcccga gccattccgg gagttggtcc atcgcgactg cgacaggggc tcgttcccct
     7381 ccctccgtga cttcagcaaa ctgcaaggcg accttgcgag tcaaccgtcc gagcatctgc
     7441 tcgagctggc gcacaccggc ttcccgcgtg taccgggtga tgatccgatc aaggacctcg
     7501 ggtgcaagta tgacttcgct gggttgcaag cctgcttcgt tcaagcggcg cggccagaga
     7561 taccgcatgg caatctcggc cttctctcgt tcgctgtagc cgcccaattg aatgatttcc
     7621 attcgatcca gcaacggctg gctgatggtg tccagcgtgt ttgcggtggt gatgaagaac
     7681 accttggaca gatcgaatgg cagatccaga tagtggtccc ggaatgtatg attctgcgcc
     7741 ggatcgagca cttccagcag tgccgcagcc ggatcgccgc gaaagtcccg gcccatctta
     7801 tccacttcgt ccagcatcag gaccggattg ttgacccccg cccggcgcac agcttggatg
     7861 atccgtcctg ggagggctcc cacataggtc cgtctgtgcc cgcgcaactc cgcttcatca
     7921 tgcactccgc cgaggctgaa tcgttcgaac ttgcgtccca tcgcacgggc gatggactgg
     7981 cccaggcttg tcttgccgac tccgggcggt cccacgagac agaggatcgg cgccttggct
     8041 cccgggttca atttcaagac ggccaagtgc tcgacgatgc gctctttgac ttccttgatt
     8101 ccatagtggt cttcatccag cacctggcgg acgcgggcga gttccaagct gtcctcagac
     8161 gcctttttcc atggcagctc gaggaccaat tcgagatagc ttcgaataac ctgatgatct
     8221 ggagaggtcg gaggaatttt agctaagcgg gtgacttcac gttcagcctc cttccgaaca
     8281 tgatccggta aattcgtctc ttcgatttgt ttcttcagtc ggcccgtctc gccttcctcg
     8341 ctgtccgact cgcccagctc ctgctgaatt gccttgagtt gttcacgaag aatgtactcg
     8401 cgttgcgact tgctgatctt agcttgtgct tcggtggtga tcttctctct caattgaagg
     8461 atctggatct cgcgtgacaa tgccgcatat aagctacgca ggagatcgac gcgtgtggtg
     8521 gcttccagaa gtttctgctc ctcctgaacc gtcaggttca acagtgacgc gatcctgtag
     8581 gcgagggcaa ctgggtcttg ttccgctccc aaggcgacaa cagcttcttc catgcctggt
     8641 gcacgaatga gtttgggcag atcggcgacg acgtcttgaa tcgcgcgatg caaggcatcg
     8701 acctcggtgc cggtatccgt cggttctgga tactcacgga cgcgagcgac caagtacgga
     8761 tccgtttgat cgagcctcag gagaacgact cgttccagcc cttgaaccat ggcatggatc
     8821 tgcccctccg atacgcgggc gatctgtttg atgacggcct tggtcccgat ggagtacaga
     8881 tctttcaatt ctggtccatc aacgtccggc gcacgttggg cgatcaccag gagcgtcttg
     8941 tcttccgttc tcatggccgc ctcgaccgcg gcaatggacc ggtctcgacc gacggtcaac
     9001 ggcatcatga cgcccggaaa cagaaccgtg cgcttgatgg gaaggaccgg cagattcgag
     9061 acgaacgact gttcactcat tttggcctcc ttggggacta tactgaatgt gtaaatgtta
     9121 aggtagccat tacttgaggt gagtcaaggt aagggagaga tccctcgccg gatcatgttg
     9181 aatacactga ctggaagagt cggatcctgc ttgaatcgat acaggagctt taaactctat
     9241 cactttgaaa ctgttggact acttatcaac gaacaatcca ccagcagtgt cttgctgaat
     9301 ggtgttgttg gtgcgtttta atgtgccaga agaattcgtg gttgggaggt ctgggtagtc
     9361 atctgttccc gcgagatcga agaaggcgcc caggctcgtg gtgtccgctg cgcccatccc
     9421 ccgtgagact cgatattggt cagcgcctcc ctcctcgatg aagacgctcc acgaggtaag
     9481 gtcagccatc ccaagaccgg tcgaatggtc gaacagatag gtgtcacggc ccttgccccc
     9541 gtcgatcatg agccccgcgc tgtggtccca agccactccg gcgttgtaca agggaccaga
     9601 tgatccataa tgatcctctc catgccggtc gatgaagagg gcggcaccaa aatgagccga
     9661 ggctccatgt ccgtaccgag ctgcttggtg atcgtcgtca ccatccaaat cgagtttcag
     9721 gcctgtgccg aagaaatatc cgtgtccttg tgagaaattg gcgctctgat agtggtcgtg
     9781 accgtcgatg tcgaggaaga ctccccaccc gccggccaga ttgtgggctt gccattcgag
     9841 tcgcttggtc agaatgcgtt ggcctgatcc tgtgccgagt ccgaaacaat cgtattgaaa
     9901 taatggatca ccgggtttcc cattgggagc gtcctgtgca ttgtaggcgc tggggtactt
     9961 gtcaccgcac tggtagtgat cgtcaccggt cacatcgaca agggcgccga ctcctaacgg
    10021 tccaccgaac ccaacggcga atccgtagct cgtgtatcga tcgttccccg cgctgtccag
    10081 cagcaaaccc atacctccaa tggcagcacc ctgcgtcaat cgatttccca tgtacgcatc
    10141 gttgcctttg ccgtcgaata aaatcccaag cccgccgaat cctgcgccac cggatccacg
    10201 ttcgagttga tagacgtcgt cgcccgcatg gtcaatcaaa aggcccacac cgagtcgccc
    10261 ggtggccagt ccaagcggcg tgccgtcata ggtatcgttc ccactgagat cgatgaccac
    10321 ggcgttgccc tgatcttcgc tggcagaagc ggcaatcgtc cctcggtaga gatcgtttcc
    10381 cccaagatca atcacaagac cgaatcgccc gtcgagctca ta
//
LOCUS       DATDNW010000044         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_73237,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000044 DATDNW010000000
VERSION     DATDNW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73237"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VI359_01165"
     CDS             <1..1137
                     /locus_tag="VI359_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY6972888.1"
                     /translation="EASDMYLTVDAPPIYRVHGSTEQTDAPPFTNEQLEALALALMRG
                     QQRSEFEEKMEMNLALYYKELGRFRVNIFRQRGNVGLVFRHIKAEIQTVEQLRLPPII
                     KDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNSIHPGHIVTVEDPIEFVHSHKKSLI
                     TQREVGFDTLSFQNALRNTLRQAPDVILIGEVRDTETMEAAITFAETGHLCIGTLHSN
                     NANQAIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSTDGKRVPALEILLDTPRI
                     KDLIKKAEVDTLKEAMEQGIEEGCQTFDHVLFQLYKEEKITLEQALINADSANNLRLK
                     IKIEGLRGDDAINALLDKKSGDPSDSAFRIQGGNSGNVTPIRKR"
     gene            complement(1182..1670)
                     /locus_tag="VI359_01170"
     CDS             complement(1182..1670)
                     /locus_tag="VI359_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972889.1"
                     /translation="MPERSVTALIVSIILLGVVVALLRSPPRSVPTSPSSSISPAPVV
                     APETIPLVTGDEPIVSIFIRAGCPVCHTIPGISGAEGRVGPPLWLGTTGAKRLVDPAY
                     QGRAKTIREYVIESVVEPDVYVVPGYPPSTMPTWYGTKLSALALEKISTYLESLYAPV
                     SP"
     gene            complement(1748..>2241)
                     /locus_tag="VI359_01175"
     CDS             complement(1748..>2241)
                     /locus_tag="VI359_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6972890.1"
                     /translation="WADALAYCKWAGKRLPTEAEWEKAARGTDGRRYPWGPMQPFVDI
                     ANYNRGVWVSETITLMGVTSGLEGMSVRHGLKEGGKSPYGLTHMAGNAAEWVADWYER
                     DYYQKSPDKNPTGPASGDKHVLRGGSWADLPTALRVTARFSAEPDFEDRTIGFRCAMS
                     ATK"
BASE COUNT          531 a          659 c          591 g          460 t
ORIGIN      
        1 gaagcctcgg acatgtattt gaccgtcgac gctcctccca tctatcgtgt gcatgggtca
       61 accgaacaga ccgacgcccc acccttcacc aacgaacagc tcgaagccct tgccctcgcg
      121 ctcatgcgcg ggcaacaacg tagtgagttc gaagagaaaa tggagatgaa cctggcgctc
      181 tattacaaag agctgggccg tttccgtgtc aatatattcc gccaacgagg aaatgtcgga
      241 ttggtcttcc gacatatcaa agctgagatc caaactgtcg agcaactaag gctgcccccg
      301 attatcaaag acattgcgat gacgaagcgt ggactagtct tggtggtcgg agcgacgggg
      361 tctggcaaat cgacctcact ggctgcgatg atcgatcatc ggaattcgat tcatcccggc
      421 cacatcgtga ccgtggaaga tccgatcgag ttcgtccaca gtcataaaaa atcgctgatt
      481 acccaacgcg aagtcggttt cgataccctc tcattccaaa acgcgttgag gaacaccttg
      541 cggcaggccc cagatgtcat tctgatcggc gaggttcggg acacggaaac catggaagcc
      601 gccatcacct ttgctgaaac cgggcacctc tgcatcggca ccttacactc gaacaacgcc
      661 aaccaagcga tcgagcgtat catgaacttc tttccggtcg aacggcatgc gcagatctat
      721 ttgcaattgt cgttgaactt gcgcgccatt atttcccagc ggctcatacc ttccacagac
      781 ggcaagcgcg tcccagcact cgagatcctg ctcgataccc cgcgtattaa ggatctgatc
      841 aagaaagccg aggtcgacac gctcaaagag gcgatggaac agggcataga agaaggctgt
      901 caaactttcg atcacgtgct gtttcagctg tacaaggaag agaagattac gttggagcag
      961 gccctgatta atgccgacag cgcgaacaac cttcgtctga agatcaagat cgaagggctg
     1021 agaggagacg acgccattaa tgcgctcctc gacaagaaat ccggtgatcc gagcgacagc
     1081 gcatttcgaa tccagggcgg caactccggc aacgtcacac ccatccgtaa gcgctagttt
     1141 catgtccgtc gccgcggcac ggcgccgtgt tgagggcgat tctacgggga aaccggcgcg
     1201 tacagggatt ccagatacgt cgagattttc tcaagcgcca acgcactcag tttcgttcca
     1261 taccatgtcg gcatggtgct aggtgggtaa ccaggaacga cgtagacgtc tggttcgacc
     1321 accgactcaa tcacatattc acggattgtt tttgctctcc cctggtaggc tggatcgacc
     1381 aaacgtttgg ctcccgttgt ccccaaccac aatggcggcc ccacgcgccc ctctgctccc
     1441 gatattccag gaatggtatg acaaaccgga cagccggccc gaatgaaaat ggacacaatt
     1501 ggttcgtcac cggtcaccaa cggaatagtt tccggggcaa cgacgggcgc cggcgagatc
     1561 gacgatgaag gagaagttgg gaccgaacgc ggcggcgatc gaagaagggc gacgaccact
     1621 cccagcaaga ttatgctcac aatcaaggcg gtgacagacc gttcaggcat gcgagaaagg
     1681 ctgacgagtg agagcgaggg accttcaacc ggacactgcc gttgttctgg tcggtaggac
     1741 ctctgcgtta cttggtggca ctcatagcac agcgaaagcc gatggttcga tcctcgaagt
     1801 ccggctcggc agaaaagcgg gctgtgactc gcaaggctgt cggcaaatcg gcccaggagc
     1861 cgccccgcaa cacatgtttg tccccgctgg ctggacctgt cggattcttg tccggactct
     1921 tttggtagta gtcacgttcg taccagtcgg cgacccattc ggctgcattg cccgccatat
     1981 gggtgagacc atagggactt ttcccccctt ctttcagccc gtgacgaacg ctcattccct
     2041 caagtccgct ggtaacgccc attaacgtaa tcgtttcgct cacccagact ccccgattgt
     2101 aattcgcgat atccacaaac ggctgcatcg gtccccacgg ataccgacgt ccatcggttc
     2161 cccgcgccgc tttttcccat tcggcttcag tgggaaggcg tttcccagcc cacttacaat
     2221 aggctaaggc atcggcccac t
//
LOCUS       DATDNW010000045         6301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1234831, whole genome shotgun sequence.
ACCESSION   DATDNW010000045 DATDNW010000000
VERSION     DATDNW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234831"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VI359_01180"
     CDS             complement(<1..331)
                     /locus_tag="VI359_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEY6972891.1"
                     /translation="MKLPFLPNHDDASFFDFLTRHHPDLTPAGRNWTASTPAPVESPR
                     AQAPMTVPQATTVLAIRYQEGVVIAGDRRATEGYQIAERRIEKVFKIDEYSAMAIAGA
                     AGPCIEMA"
     gene            complement(365..1864)
                     /gene="dop"
                     /locus_tag="VI359_01185"
     CDS             complement(365..1864)
                     /gene="dop"
                     /locus_tag="VI359_01185"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEY6972892.1"
                     /translation="MPDVKPPTTSRVLGTETEFGIASRDAAASDPVSNSIAVIGHYPG
                     LPAPQAIWDYENENPLLDARGFEVDGERERPNPDYNRQLNKVLANGGRLYVDGAHPEY
                     SIPECNNAREVVAFERIGERILAQSLDRMTSARGRELCVVYKNNSDGKGNSYGYHENY
                     LVSRSVPFDRLVKVLAPFFVTRPIYAGAGKVGAENQTSPAEYQISQRADFFECLVDLN
                     TMVRRPIVNSRDEPHADSGKYRRLHVIVGDANMAEVATYLKVGTLNIVLELIEAGADL
                     PQFDLEDPVFAIKQVSRDLTMNGTLRLTGGHATTAVAIQRAYLKAAMNFYACHEISQV
                     TKDILVRWEEVLEKLERDPRTLVRELDWVAKRHLIESYMERKGCGWDDPRVRLMDLQY
                     HDVRQDKGLYYTLERSNMIERVVQETEIVRAEMTPPPGTRAYFRGRCAGKFGKSLYGA
                     SWTSVLFDIGNTTIKKVPLMDPLRGTESLTAELFEQSDSAASLLEKLKA"
     gene            complement(1876..3552)
                     /gene="arc"
                     /locus_tag="VI359_01190"
     CDS             complement(1876..3552)
                     /gene="arc"
                     /locus_tag="VI359_01190"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEY6972893.1"
                     /translation="MIHRNDEHAREVDKLRVQIQSMEDELRRLYQSRYQLDQATKQNE
                     KLVATLQEAKAQIEALRAEVEKLTAPPSTYAIFSSLNPDGTGNVYVSGRKMKVSLHPS
                     IKAKALRKGQEVVLNEALNVIEVKAFDVQGEVVRLKDVLEGNRALVTLHFDEEKVAEL
                     GDPLLLERLSVGDHLLYDPRSGCVIEKLPKSEAEELVLEEVPDVDYAHIGGLQHELEQ
                     VRDAVELPFLHPTLFSEFKLSAPKGVLLYGPPGCGKTLIAKAVANSIAKKLGHLTGKE
                     VRSYFLHVKGPELLNKYVGESERQVREVFKKAKERAADGNPVIVFFDEMDALFRTRGT
                     GISSDVESTIVPQFLSEIDGVERLRNVIVIGASNRQDLIDPAVLRAGRLDVKVRVGRP
                     DAAAAKDIFSKYLNPDLPFAEDEVKRHGGDAQALVEHLTSMTVEAMYATSEENKFIEV
                     TYANGEKEILYFKDFASGALIEGIVARAKKYAVKRAIAQEGRGLRSEDLIRAIREEFK
                     EHEDLPNTTNPDDWAKVAGKKGEKIVHLRTISGGSTESRQIETMTTGHYL"
     gene            complement(3777..4412)
                     /gene="thiE"
                     /locus_tag="VI359_01195"
     CDS             complement(3777..4412)
                     /gene="thiE"
                     /locus_tag="VI359_01195"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY6972894.1"
                     /translation="MPQLNSRLLVVTDRRQTGGRPLLDVLTSALAAGVRAVQLRERDL
                     SSRDLLRLAQEVQRLTIASGAQLVINDRVDVAMTLDNVGVHLRANSLPVAVARKLLGG
                     QRLIGISTHAVDEVVRAEAEGADYVVLGPIYETPSKTAYGAPLGVRALEEACRRVRVP
                     VIGIGGVTAARARDVRHAGAFGVAVVTAVLGAVDVERAVRELLDAVSMSSS"
     gene            complement(4520..5296)
                     /locus_tag="VI359_01200"
     CDS             complement(4520..5296)
                     /locus_tag="VI359_01200"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY6972895.1"
                     /translation="MNTNDRLTIAGREFKSRLWVGTGKYKNFIETQKAIEASGADVVT
                     VAVRRVNITDRSKENLLDYLDPKKYVILPNTAGCYTVEDAVRYARLARAAGISDLVKL
                     EVLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAP
                     IGSGLGIRNPYNLKIIMEQVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQD
                     PIAMAEAMKYAVYAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            complement(5293..5559)
                     /gene="thiS"
                     /locus_tag="VI359_01205"
     CDS             complement(5293..5559)
                     /gene="thiS"
                     /locus_tag="VI359_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY6972896.1"
                     /translation="MVESIQIQVNGESRPWRAGATVGELLRELDITSERVAVELNLEI
                     LDRKEFDRRGIKDGDRLEILSFIGGGSLNKKITPMTGDVIRSGR"
     gene            5679..6149
                     /locus_tag="VI359_01210"
     CDS             5679..6149
                     /locus_tag="VI359_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY6972897.1"
                     /translation="MFKHNQQVTTLFRSMADLLTARRENPYRIRAYRRAADGIEALEE
                     DVAEIAKRQALEDIDGIGRDLADKIVEILQTGTFQAYEALRTPLPDEVKHWASLPGLS
                     ESLVSYLYARLNIRTLPALEQLVQSHLLRTIPGFSGSEETLLHAIQQLRTSQRA"
BASE COUNT         1163 a         2001 c         1767 g         1370 t
ORIGIN      
        1 tggccatttc gatgcacggc ccggcggctc cggcgatcgc catggcggaa tactcgtcga
       61 ttttgaacac cttttcgatc cgccgctcgg cgatctgata cccttccgtc gcgcgccgat
      121 cgcccgcaat caccaccccc tcctggtatc tgatcgccag gaccgtcgtg gcctggggca
      181 ccgtcatcgg cgcttgcgca cgaggcgatt cgacgggcgc cggcgtcgag gccgtccagt
      241 tccttcccgc aggagtcagg tcgggatgat ggcgggtcag gaagtcgaag aaactcgcgt
      301 cgtcgtggtt tggtagaaat ggcagcttca tggtggcgtc aggagggaat gtggcccact
      361 gcgatcacgc tttcagcttc tccagcaacg acgccgcgct gtctgactgt tcgaacagtt
      421 cggccgtcag cgactcagtc ccgcgaaggg ggtccatgag cggaaccttc ttaatggtcg
      481 tattgccgat gtcgaagagg acggacgtcc agctcgctcc gtacaaggac ttgccgaact
      541 tcccggcgca ccggccgcgg aaataggcgc gagtccccgg gggcggtgtc atttcggccc
      601 gcacgatttc ggtctcctgc accacgcgct cgatcatgtt gcttcgctcc agcgtgtagt
      661 agaggccttt gtcctgacgg acgtcgtgat actgcagatc catgagccgc acgcgggggt
      721 cgtcccatcc gcaccccttc cgctccatat acgattcgat gagatggcgc ttggccaccc
      781 aatccagctc gcgaaccagg gtgcgaggat cccgctcgag tttctccagc acctcttccc
      841 agcggaccag aatatccttc gtcacctggc tgatttcgtg acaggcatag aaattcatcg
      901 ccgccttcaa gtacgcgcgt tgaatcgcca cggcggtggt ggcatgacct ccggtgagtc
      961 tgagggtgcc gttcatcgtc aggtcgcgcg acacctgctt gatcgcgaag accggatcct
     1021 ccagatcgaa ctgcgggaga tccgcgccgg cctcgatcag ctcgagcaca atgttcagcg
     1081 ttccgacttt cagatacgtc gcgacctccg ccatattggc atcgccgacg atgacgtgca
     1141 gccggcgata ttttccagag tccgcatgcg gctcgtcgcg cgagttgacg atcggccggc
     1201 gcaccatcgt gttgagatcg acgaggcatt cgaagaaatc ggcgcgctgg gagatctggt
     1261 actcggccgg actggtctga ttttccgctc cgacctttcc cgccccggca tagatcggcc
     1321 tggtcacgaa gaacggcgcc agcaccttga ccagtcggtc gaacggcacc gatcgggaga
     1381 cgagatagtt ctcgtgatag ccgtagctat tgcctttccc gtccgagttg ttcttgtaga
     1441 cgacgcacag ttcgcgccct ctcgcactgg tcatgcgatc gaggctctgg gcgagaatgc
     1501 gctccccgat ccgttcgaac gccacgactt cccgcgcgtt gttgcattcg ggaatcgagt
     1561 actcgggatg ggctccatcg acgtagagac gccccccgtt cgccagcacc ttattgagtt
     1621 gccggttgta gtcgggattg ggccgttccc gctccccatc cacttcaaac ccgcgggcat
     1681 cgagcaacgg gttttcgttt tcgtaatccc agatggcttg cggcgccggc agtcccgggt
     1741 agtgcccgat cacggcgatc gagttcgaca cgggatcgga ggctgcggca tcgcgtgacg
     1801 cgatcccgaa ctccgtttcc gttcccagta cgcgagaagt cgtcggcggt ttcacgtctg
     1861 gcatggagtc aggtcttaca gataatggcc cgtcgtcatg gtttcaatct ggcgcgactc
     1921 ggtcgatccg ccgctgatcg tccggaggtg gacgatcttt tcacctttct tgccggcgac
     1981 cttggcccag tcatcggggt tggtcgtatt ggggaggtct tcgtgctcct tgaactcttc
     2041 ccggatcgcg cggatcagat cttcggatcg cagcccgcgg ccttcctgcg caatcgcgcg
     2101 tttgaccgcg tacttcttcg ctcgagcgac gatgccttcg atcaacgcac cgctcgcaaa
     2161 gtccttgaag tagagaatct ctttctcgcc gttcgcgtag gtcacctcga tgaacttgtt
     2221 ttcctctgac gtggcgtaca tcgcctccac ggtcatcgag gttaagtgct cgaccagcgc
     2281 ctgcgcgtct ccaccgtgac gcttcacctc gtcctcggcg aagggaagat ccgggttcag
     2341 atacttggag aagatgtctt tcgccgccgc ggcatccgga cgcccgaccc tgaccttcac
     2401 gtccagccgg ccggcgcgca agacggcggg gtcgatcaaa tcctgccgat tgctcgcgcc
     2461 gatgacgatc acgttccgca gccgctcgac gccgtcgatc tcggacagga actgcggcac
     2521 gatggtcgac tcgacatcgg aggaaatccc cgttcctctc gttctgaaca gtgcgtccat
     2581 ttcatcgaag aacacgatga cgggattgcc gtccgcggcc cgttccttcg ccttcttgaa
     2641 gacttcgcgc acctggcgct cggactcgcc gacatattta ttcagcaact ccggcccctt
     2701 cacgtgcagg aagtagctcc ggacttcttt gcccgtcaaa tgcccgagct tcttcgcaat
     2761 ggaattcgcc acagccttcg cgatcaacgt tttcccgcag cccggcggtc cgtagagcaa
     2821 cacacccttc ggcgcgctga gcttgaattc cgagaagagc gtcgggtgca ggaacggcaa
     2881 ttccaccgcg tcgcgcacct gttcgagttc atgctgcaac cccccgatgt gggcgtaatc
     2941 gacatcgggc acttcttcca ggaccaattc ctcggcctcg gacttcggca gtttttcaat
     3001 gacgcagccc gaccgcgggt cgtagagcag gtgatctccg acactcagcc gctcgagcag
     3061 cagcggatcg cccaactcgg cgactttttc ttcatcaaag tgcagggtca ccaacgcgcg
     3121 gttgccttcc aacacatctt tcagcctgac cacttctccc tgcacatcga acgccttgac
     3181 ttcgatgaca ttgagcgcct cgttcaggac gacctcttgc cccttgcgga gcgctttcgc
     3241 tttgatcgac ggatgcaggc tgaccttcat cttccgtccg gaaacataaa cgttcccggt
     3301 tccgtccgga ttcaaactgg aaaagatggc gtaggtggac ggcggcgccg tcagcttttc
     3361 gacttcggcg cgcaaggctt cgatctgagc cttggcttcc tgcagcgtgg cgaccagttt
     3421 ctcgttttgt ttggtggcct ggtcgagctg gtagcgggat tgatacagcc ggcggagctc
     3481 gtcttccatc gactggatct gcacacgaag cttgtcgact tcacgggcat gttcgtcgtt
     3541 tcggtggatc acgtccgttt ctccctcgga taatcgattc gtgatgtgcc tgacagaatc
     3601 tctgagggac cggagtcggc tcggactttc tttcttctca cccatgaaga ggctcgcgga
     3661 ctcgcatcaa tccttatcaa gatcgagaag tccaagtctt ggcggattct atcatccgtt
     3721 tttttcacgg tcaactggac tgcaagatct cgcacgacga tgtgaacgca tcatgatcat
     3781 gacgacgaca tcgacaccgc atcgagcaac tctcgcaccg ctcgttcgac atcaaccgca
     3841 ccgagcacgg ccgtcaccac cgcgacaccg aacgcacctg cgtgtcgcac gtcgcgcgca
     3901 cgcgccgcgg tcactccgcc gatgccgatc accggcacgc ggacacgtcg acacgcttct
     3961 tcgagcgcgc gtactccgag tggagcgccg tacgcggtct tcgacggcgt ttcataaatg
     4021 gggccgagga cgacgtagtc cgctccttcg gcttcggccc gcactacttc gtcgacggca
     4081 tgcgtcgaga ttccgatcag gcgctgtcct cccagcaatt tcctcgccac ggcgaccggg
     4141 agactattcg cacgcaagtg cacgccgacg ttgtcgagcg tcatcgcgac gtcgacgcgg
     4201 tcattgatca caagctgggc gcctgaggcg atcgtgagtc gctgcacctc ctgcgcaaga
     4261 cggagcaggt cgcgcgaaga aagatcgcgc tcgcgaagct ggacggcacg gacgccggcc
     4321 gcgagtgcgc tcgtcaacac atcgagcagc gggcgtccac cggtctgccg acgatcggtc
     4381 accacgagga gccgggagtt aagctgaggc atgattcgtc aggcgtgaag cgtgaggcgt
     4441 gaggcgttac tatagtctcg gaagggaatg tcttcgaata gcctcttcgc ctgacgtttc
     4501 acgcctaacg ccttacgtct tacagcattc cctcgatggg gctgctggcc gtggcgtaca
     4561 actttcgtgg aatgcgtccg gctttgtagg ccagacgtcc ggcatacacg gcgtacttca
     4621 tcgcttcggc catggcaatc ggatcctggg caccggcaat cgccgtgttc atgagcaccg
     4681 catcggctcc atactccatc gccagcgctg cgtcggatgc cgtcccgaca ccggcgtcga
     4741 cgatgatggg aaccttgacc tgctccatga tgatcttgag attgtaggga ttacgaatgc
     4801 cgagaccgga tccgatcggc gccgccagcg gcatcacagc gggacagccg atatccacca
     4861 gcttcttcgc gacgatggga tcgtcgttcg tatagggcag gacgatgaat ccttccttga
     4921 tcagaatttt ggcggcttcg atgagccccg ctgtatccgg aaacaaggtt ttctcgtcgc
     4981 ccaacacttc aagcttgacc agatcggata tgcctgccgc ccgcgcgagc cgcgcatagc
     5041 gcaccgcgtc ttcgacggta tagcacccgg ccgtattggg cagaatgacg tacttcttgg
     5101 ggtcgagata gtcgagcaga ttctctttgg atcgatccgt aatgttgacc cggcggactg
     5161 cgaccgtcac gacatcggcc cccgaagctt caatcgcctt ttgcgtttcg ataaaattct
     5221 tgtacttgcc ggttcccacc cacaagcggg acttgaactc tcgtccggca atcgtcagcc
     5281 gatcattggt attcatcgtc cactccttat cacatcgcct gtcatcggag tgatcttctt
     5341 attcagactt cctcccccga taaagctgag aatttccaat cggtcgccgt ctttgatccc
     5401 gcgccgatcg aactctttgc gatcgaggat ttccaggttc aactcaacag cgacgcgttc
     5461 gctcgtgata tccagttcgc gcaacaattc tccgaccgtc gcccccgccc gccagggacg
     5521 tgactccccg tttacctgga tctgaatgga ttcgaccatg cccgtcatcc tatgggacca
     5581 gaaaaccggt tgtcaacaaa cccgataccc cgctggccct cttgcctccg gccctgagac
     5641 ggtcgcacaa tacatcatta aatcgcttgg caagctctat gtttaaacac aaccaacaag
     5701 tgaccacgct cttccgatcc atggccgact tactgacggc gcggcgcgag aatccctatc
     5761 ggatcagggc ctatcggcgc gcggccgatg gcatcgaggc gcttgaggag gacgtggcgg
     5821 agattgccaa acgacaggcg ctggaagata tcgacgggat cggccgggac ttagcggata
     5881 agattgtgga gatcctgcaa acggggacgt tccaggccta cgaggcactg agaactccgc
     5941 ttcccgatga agtcaaacat tgggccagct tgccggggct ttcagaatcg ctcgtgagct
     6001 acctgtacgc ccgcctcaac attcgaacgc tgcccgccct tgagcagctc gtgcaatcgc
     6061 atctgcttcg cacgatcccc gggttttcgg gatcggaaga gactcttctg catgccatcc
     6121 aacagctgcg cacatcccaa cgagcttagg attgcgcctg agggagtccc ttgaaaatct
     6181 tccagctctt caacagctcc actgccttct gaagctggac gtcctgctcc agtgacggct
     6241 cgcccgccac atcaaacggt ggcggcgtcg gcgtcgtccc gttcttggct ccctcttctg
     6301 a
//
LOCUS       DATDNW010000046         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1166529, whole genome shotgun sequence.
ACCESSION   DATDNW010000046 DATDNW010000000
VERSION     DATDNW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166529"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /gene="pheT"
                     /locus_tag="VI359_01215"
     CDS             <1..1596
                     /gene="pheT"
                     /locus_tag="VI359_01215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY6972898.1"
                     /translation="KGQSPETGELRIELQDSNRPDLWCCEGIARQIRVKQQGKLGPYP
                     FLKLRTRSRKQLTVAPGLESIRPYVAACAATGYRVTQDGLTQLIQTQEKLADIFGRKR
                     HTVSIGLYRLSQIEFPVTYDLVKSDEAKFTPLGLDTAMTLGEILLVHPKGLEYGNIVA
                     GQERLPLLRDAKRQVLSFPPIINSRDIGEVRVGDDALFVEVTGTDLAMVILTLNIFAM
                     NLADRGAIIEPIEVAYPYKTEQGTKIVTPLDLAKGRTLPISTIEQALGQPLGVQPIKQ
                     ALEGYGYEVTTARDTITVTLPPYRQDLMHAMDVVEDVAISRGYGDFQPIMPSQFTVGG
                     LSQVETTSDRVRDLMVGMGFQEVISNILGSPEAFCEAMRLNGTEWGQLVEVDNVMSQS
                     YSALRQWILPSLLRVEAASSRAFYPHRLFEVGEVARPDAAHEMGSQTVAVLGVLLVHA
                     TAHFSEIHSCVDTLFYYLNQSYQLEPLQHPSFLEGRAGRIVSQGKYVGLIGELHPEVL
                     ERWQITVPAAAFEVDVTQLTERL"
     gene            complement(1661..2029)
                     /gene="rplT"
                     /locus_tag="VI359_01220"
     CDS             complement(1661..2029)
                     /gene="rplT"
                     /locus_tag="VI359_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY6972899.1"
                     /translation="MPRVKGGSKTRQRRKKRLKLAKGQYGGKSRLFRTATESVDKGQT
                     YAYTGRKNRKRDFRQLWIARISAGVRAHGLTYSRFINALKKANVLLDRKVLSDMAIKD
                     STGFERLIDLAKQHLVPAGA"
     gene            complement(2077..2268)
                     /gene="rpmI"
                     /locus_tag="VI359_01225"
     CDS             complement(2077..2268)
                     /gene="rpmI"
                     /locus_tag="VI359_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY6972900.1"
                     /translation="MKVKTHSGAAKRFAKSGSGKIMRRQAGKRHILTSKRRNRKRRLS
                     GTVEVDATKTLALRRLLPS"
     gene            complement(2310..2801)
                     /gene="infC"
                     /locus_tag="VI359_01230"
     CDS             complement(2310..2801)
                     /gene="infC"
                     /locus_tag="VI359_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY6972901.1"
                     /translation="MNREIRVREVRVIGPEGEQLGILPTQEAFKQAQETGYDLVEVAP
                     TSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKIRQIKG
                     FLEEGNKTKVTLTYRGREMANQEMGRTVMNSVIEQLAETGTIEYAPRMEGRSLIMIVA
                     PKS"
     gene            complement(2830..>4468)
                     /gene="thrS"
                     /locus_tag="VI359_01235"
     CDS             complement(2830..>4468)
                     /gene="thrS"
                     /locus_tag="VI359_01235"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY6972902.1"
                     /translation="DSFYYDFAYDRPFTPEDLEKIEARAREIIKRNLPIKRSEFSKRQ
                     AIEFFRVRGEAYKVELIEGFPDNESISAYSQGAFVDLCRGPHLPTTGHVGAFKLLNTA
                     GAYWRGDERNPMLQRIYGTSFPTQAELDAHLARLEEIKRRDHRKVGKELDLITIQDEI
                     GPGLVLWHPKGALIRLLIENFWREQHLQNGYDLVYSPHVARLDLWKTSGHVDYYRDNM
                     FTPMKVEASEYQLKPMNCPFHIMIYKSHLRSYRDLPIRYGELGTVYRYERTGVLHGLL
                     RVRGFTQDDAHLFCRPDQIEGEVSRVLDFTFFILRTFGFTGFEVYLSTKPEKAVGSEE
                     RWAQATSALEAALKGRGVAYEIDPGEGVFYGPKIDIKIKDALGRSWQCSTIQVDFNNP
                     ERFELGYIGEDGKSHQPIMIHRALMGSIERFFGILIEHFGGAFPTWLAPVQATVLSIT
                     DNQRDYVAGVVAQLKAAGFRAEADLRNEKIGFKIREAEKAKVPFMLVAGDREVQSGTV
                     SVRGRSGSNLGNMSISGFVEHLGQEVRRQTRDLQPTN"
BASE COUNT          949 a         1250 c         1199 g         1070 t
ORIGIN      
        1 aaaggccaaa gtcccgagac cggcgaattg cggatcgaat tgcaagacag taaccgtcca
       61 gacctctggt gctgcgaagg cattgcccgt cagattcgcg tgaaacaaca ggggaagctc
      121 ggtccctacc catttctgaa actccgtact cgttcacgga agcaactgac tgtcgcgcct
      181 ggcttggaat ctattcgccc ttacgtagcg gcttgtgcgg ctaccggcta tcgtgtgaca
      241 caagacgggt tgacgcagct cattcagaca caagagaaac tggcggacat tttcggacgg
      301 aagcggcaca ccgtctcaat cgggctctat cgactctctc aaatcgaatt tcccgtaacc
      361 tatgacctag tgaagtccga cgaggccaag ttcacaccgc tcgggctgga tactgcaatg
      421 accttgggag aaattttgct cgttcatccg aagggccttg aatacgggaa catcgttgca
      481 gggcaagagc gtcttccgct gctacgggat gcaaaacgtc aggtgttgtc cttcccgcca
      541 atcatcaata gccgagacat tggcgaagtg cgtgtcggcg acgacgcact gtttgttgag
      601 gtgacaggaa ccgatctggc catggtcatc ctgacgctga atatcttcgc gatgaatctt
      661 gccgatcgtg gggccatcat cgaaccgatc gaagtcgcgt acccgtacaa gaccgaacaa
      721 ggaacgaaga tcgtgacgcc gttagacctg gccaagggcc gcacgctccc aatcagcacg
      781 attgaacagg cactgggaca gcccctgggt gttcagccaa ttaagcaggc gctggagggc
      841 tacggctatg aagtcacgac tgcgcgagac acgatcactg tcacgctgcc tccgtaccgc
      901 caagatctca tgcatgccat ggacgtcgtg gaagatgtgg ccatcagtcg cgggtatggc
      961 gactttcaac cgatcatgcc ctcccagttc accgtcggtg gactctcaca ggtggaaacg
     1021 acgtccgatc gagtcagaga tctcatggtg gggatggggt tccaagaggt catctcgaat
     1081 attttaggat ctccagaagc attctgcgag gcgatgcgat tgaacggaac ggaatggggg
     1141 caactggtcg aagtcgacaa tgtcatgtcg cagagttact cggctctccg ccaatggatc
     1201 ctcccctcac tgctacgagt cgaagccgcg tcgagccgtg cgttttaccc ccaccggttg
     1261 tttgaagttg gtgaagtcgc gaggcccgat gccgctcatg aaatgggctc gcaaaccgtc
     1321 gccgtgctag gggtgcttct cgtccatgcg acggcccatt tctcggagat ccattcgtgc
     1381 gtggacacgc tgttttacta tttgaatcag tcctaccaac tggaaccgct tcagcatccc
     1441 tcgttcttag aaggacgagc aggtcgtatc gtatcgcaag ggaagtacgt cggactgatc
     1501 ggagaactcc accccgaagt actcgaacgc tggcagatta cggtgccggc ggcggcgttc
     1561 gaagtcgacg tcacgcagct caccgaacgg ctctgaggag agcaaggggc caaccgtcct
     1621 caggcttggt cgagactcac aacccaacga acagagggta ttaggcgccg gcaggaacaa
     1681 ggtgctgctt ggcgagatcg atcaggcgct cgaacccggt cgagtccttg atcgccatat
     1741 ctgacagcac ttttctgtcc aacagcacgt tcgctttctt cagtgcattg atgaaccgac
     1801 tgtacgtgag tccatgcgca cgaacgccgg cactgatgcg ggcaatccag agttggcgga
     1861 agtcccgctt acgatttttc cggccggtgt aggcataggt ttgccccttg tctacggatt
     1921 ccgtggcggt gcggaacagc cggcttttcc ctccatactg tcccttcgcc agcttcagtc
     1981 gtttcttccg tcgttgtcgt gttttcgaac cacctttgac gcgaggcatg actcagtact
     2041 ccttttctaa tagcgtgatc gaaagctcgt tctcaatcaa gagggcagta gccgcctcag
     2101 tgcgagggtc ttggttgcat cgacctcgac ggtcccgctg agccgtcgct tgcgattccg
     2161 tcttttactc gtcaggatgt gccgtttccc tgcttgtcgg cgcatgattt ttccgctgcc
     2221 cgatttcgca aatcgcttcg ccgctccact gtgggttttc actttcatga tgcgtgaatc
     2281 ctttggttaa aataattcat attggcaatc taactcttcg gcgccacgat catgatcagg
     2341 ctccgtcctt ccatacgcgg cgcatactcg atggttcctg tctcagctaa ttgttctatg
     2401 acggaattca tgaccgtgcg tcccatctcc tgattggcca tctcccgacc ccggtacgtc
     2461 agtgttacct tcgtcttgtt tccttcctcg agaaatccct taatctgccg gatcttgatc
     2521 tcgagatcgt gtttgtcggt cctggggcgg agcttgatct ccttcacctg cgtcgatttc
     2581 tgatgcctgc ggctttggtg gtccttcttg ctgagttcat atttatattt cccgtagtcc
     2641 ataatgcgac agacgggagg gaccgatgtt ggcgccactt ccactagatc atatcccgtt
     2701 tcctgggcct gcttgaaagc ctcctgcgtg gggagaatgc caagctgttc tccctcagga
     2761 ccgatcaccc gaacttcgcg cactctgatt tcacgattca ctcgcaattt gggaacgata
     2821 ggccacctct caatttgtgg gttgaagatc tcgggtttgt cgcctgactt cttgtccgag
     2881 gtgttcgacg aaccccgaaa tgctcatgtt acccaagttg cttccactgc gtccacggac
     2941 ggagacagtc ccgctctgga cctcccgatc gccggccacc agcatgaatg gcactttggc
     3001 cttctcggct tcccggattt tgaatccgat cttttcatta cgcaggtcgg cctctgcccg
     3061 aaaacccgcg gctttcaatt gtgccacaac tccggcgaca tagtccctct gattatccgt
     3121 gatcgagagg acggtggcct gaacgggagc gagccaggtc gggaaggccc cgccgaagtg
     3181 ctcgattaag ataccgaaaa agcgttcaat ggatcccatc agggcgcggt ggatcatgat
     3241 tggctgatga gacttaccat cttccccaat atatcccaac tcaaaccgtt cgggattatt
     3301 gaagtcgact tggatggtgg aacattgcca agaacggccc aacgcatcct tgatcttgat
     3361 gtcgatcttc ggtccgtaga aaacgccttc tcccggatcg atctcgtacg ccacgccacg
     3421 tcccttgagg gctgcttcga gcgcgctcgt cgcctgcgcc cagcgctcct ccgagcccac
     3481 cgccttttct ggcttggtcg ataagtagac ttcgaatccg gtaaatccaa aggttctaag
     3541 aatgaaaaac gtgaaatcca agacgcggct gacttcacct tcaatttgat ccggtcgaca
     3601 gaacaaatgg gcgtcatctt gggtaaagcc ccgcacccgc aataagccat ggaggacgcc
     3661 tgtccgctcg taccggtaga cggtgcccag ctcgccatag cgaatcggca agtcgcgata
     3721 gctgcggaga tgggatttat agatcatgat atggaagggg cagttcatcg gtttgagctg
     3781 atactcgctg gcttcgacct tcatgggggt gaacatgttg tcgcggtaat agtcgacgtg
     3841 accgctggtt ttccagaggt ccagccgagc tacatgaggg gaatagacga ggtcgtatcc
     3901 gttctggaga tgttgctcgc gccaaaagtt ttcgatcagc aagcgaatca gtgcaccctt
     3961 agggtgccaa agaacaaggc cgggcccaat ctcgtcttgg atcgtgatga gatcgagctc
     4021 cttaccgact ttgcgatggt cccggcgctt gatctcttcc agcctggcga gatgggcatc
     4081 aagttcggct tgcgtgggaa acgatgttcc ataaatgcgt tgcaacatgg ggttgcgctc
     4141 gtctccacgc cagtacgcgc cagctgtatt cagaagcttg aaggctccca catgaccagt
     4201 ggtcggaagg tggggaccgc ggcaaagatc gacaaacgca ccttgactgt aagcagatat
     4261 agattcgttg tccggaaacc cttcaatcaa ttcgaccttg tatgcttcac cgcgcaccct
     4321 gaagaattcg atcgcctgcc gcttggaaaa ctcgcttctc ttgatcggga ggttgcgttt
     4381 gataatttct ctggctcggg cttcaatttt ttccagatct tctggggtaa aaggacggtc
     4441 ataagcgaag tcgtaataaa aactgtcc
//
LOCUS       DATDNW010000047         5321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1166581, whole genome shotgun sequence.
ACCESSION   DATDNW010000047 DATDNW010000000
VERSION     DATDNW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166581"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /gene="kdpA"
                     /locus_tag="VI359_01240"
     CDS             complement(<1..1701)
                     /gene="kdpA"
                     /locus_tag="VI359_01240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY6972903.1"
                     /translation="MAMNGLVQIGLFFFVLLALVKPLGWYMARVYQGQSCGLERVLGF
                     VERGIYRLCGVHPNEEMDWKTYGVAMLLFNAAGLFVLYGLQRVQGWLPFNTQGLGGVV
                     PDLAFNTAVSFATNTNWQAYGGETTLSYLTQIVGLTVQNFVSAATGMAILVALIRGLG
                     RRSTQTIGNFWADLTRTTLYILLPLAAILSVLLVSQGVVQTFTPYQTATLLQPTDYLK
                     PIMDANGQPVLDERGQPKTEPATATEQILAVGPAASQIAIKQLGTNGGGFFNVNSAHP
                     FENATPWSNFLEVLAILLIPAALCYTFGHMVGDTRQGWAILAAMTILLISLIPLGLWA
                     EQSGNPAFTAMGVDQHLRAEQSSGNMEGKETRFGIANSVLWSAATTAASNGSVNSMHD
                     SYTPLGGLVPLFLMQFGEVVFGGVGSGLYGMIVFAIIAVFVAGLMVGRTPEYLGKKIE
                     PYEMKMASLLILIMPMIVLGLTALAVSTNAGTSSLLNPGPHGFSEILYAYTSQGNNNG
                     SAFAGLNVNTPFYNLTGGVSMLMARFWLAIPTLALAGSLARKKSVPVGPGTLPTHTPF
                     F"
     gene            complement(1701..1790)
                     /locus_tag="VI359_01245"
     CDS             complement(1701..1790)
                     /locus_tag="VI359_01245"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEY6972904.1"
                     /translation="MNLMYVLAGILSFALLVYLMIALLKAEWF"
     gene            complement(2204..3418)
                     /locus_tag="VI359_01250"
     CDS             complement(2204..3418)
                     /locus_tag="VI359_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6972905.1"
                     /translation="MGQIVMWITRVVMLALWAAATPSLAEEPVGQTDHQSGDGVPASS
                     EDSKSRQPEWHYGGFLDLGYSLDFNFPENHQFRGRSTTPRVNELDVNMGGVYVRKDAT
                     EQSRWGMELMGQGGQDAKDFGFGVNLPKVQGSDTLRQFGRANVSYLAPVGNGLMLQVG
                     LFNSFIGYESLYAKDNFNYTRAWIADYSPYLMFGANAVYQFNEKWTGTFFIINEYFHL
                     QNANSLPSYGGQVAYKPNQAWTIKETVYYGPDQSNTSLEFWRLFSDSIVEWKVNDDVT
                     IAGEYQVGTQEMADQTGNPRAFYMGAALPMRWHISGPWSVALRPEVYWDRNGLMTGSE
                     QLIRAVTTTTEYKLPYKWANMILRLEYRYDESTGPGGGFFKDGQVSPGVVGLTAAQHM
                     LIFAAIWTFDSP"
     gene            complement(3912..4385)
                     /locus_tag="VI359_01255"
     CDS             complement(3912..4385)
                     /locus_tag="VI359_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEY6972906.1"
                     /translation="MASIARALVLAAGVTYVALYGVVAGADSPTRFTLVLDGTGVKDN
                     QTGLVWEQEPDRVHDVWSASVARCATKTIGGKSGWRAPSVDELKTLIDSSQHDPALPA
                     GHPFSNIKSEIYWTATPHPTDDIVAWQISFFSGEPVTDQKSGTRRMWCLLGESAK"
     gene            complement(4456..5208)
                     /locus_tag="VI359_01260"
     CDS             complement(4456..5208)
                     /locus_tag="VI359_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972907.1"
                     /translation="MASPATATVVCPKCQFEQQGGAVDCPKCGIVFAKYRPIETNVQV
                     QHQASMPTRSPWVILFNEWFVEADQKDDSLTFAGRSLILLVLLWWGWRFITTPLETNY
                     TGESFLHLINLPFHEAGHLLFMPFGRFMMFLGGTLGQILMPIVCLATFLIKTRDPFGA
                     SVALWWTAENFMDIAPYVNDARALDLMLLGGVTGKETDGHDWENILSLLGWLPYDHHI
                     AHLCYNVGILLMVASFLWGGLLIFRQHRRLFS"
BASE COUNT         1240 a         1534 c         1456 g         1091 t
ORIGIN      
        1 aaagaacggg gtgtgagtcg gcaatgtgcc tggaccaacc gggaccgatt ttttccgagc
       61 caaagatcca gccagtgcca gagtcggaat cgcgagccag aaccgggcca tcaacatcga
      121 gacgccgccc gtaagattgt agaagggagt attgacgttg agcccggcga aggcactgcc
      181 attgttgtta ccttgtgacg tgtatgcgta cagaatctcg ctaaagccat gcggccccgg
      241 attcaacagc gacgatgtgc cggcattggt gctgaccgcg agcgccgtga gtccgaggac
      301 aatcatgggc atgatcagga tcagcaacga ggccatcttc atttcatagg gctcgatttt
      361 ctttccgaga tattcaggcg tcctccccac catcagtcca gcaacgaaca ccgcgatgat
      421 ggcgaagacg atcatgccgt agagtccgga accgactccg ccgaacacga cttcaccaaa
      481 ctgcatcaga aaaagcggca ccagcccgcc caaaggagta tacgaatcgt gcatcgaatt
      541 cacggagccg tttgaagcgg cggtcgtcgc ggcagaccac aggacggagt tcgcgattcc
      601 gaatcgcgtc tctttgccct ccatgttccc gctggattgt tcggcgcgga gatgctgatc
      661 aacccccatc gccgtgaagg ccggatttcc ggattgctcg gcccagagcc ccaagggaat
      721 gagagagatt aggagaattg tcatggcggc aagaatcgcc catccctggc gtgtatctcc
      781 gaccatgtgt ccaaacgtat agcagagcgc cgcgggaatg agcaggatcg ccaggacctc
      841 gaggaagttc gaccagggcg tcgcattctc gaatggatgg gcggaattca cattgaagaa
      901 gccgccgccg ttggtcccca gttgcttgat cgcaatttgc gacgcggctg gtccgacggc
      961 cagtatctgt tctgtcgccg ttgcgggctc tgtcttcggc tggccccgtt catccagcac
     1021 cggctggccg ttggcgtcca taatcggttt gaggtagtca gtcggctgca acagcgtggc
     1081 tgtctggtat ggggtaaagg tctgcacgac cccctgcgag actaaaagca cggacaggat
     1141 ggccgcgagc gggaggagga tatacaacgt tgttcgagtc aagtcagccc agaagttgcc
     1201 gatggtctgc gtgctgcgtc gccccaaccc cctgatcagg gcgacaagga tggccattcc
     1261 cgtcgcagcc gagacaaagt tttggaccgt gagtcccacg atctgggtca gatagctgag
     1321 cgtcgtctcc cctccatacg cctgccagtt ggtgttggtc gcgaaactca cagccgtgtt
     1381 gaacgccagg tcggggacga cgccgccaag accctgtgtg ttgaatggaa gccacccctg
     1441 aacacgctgc aggccataga gcacgaacag gcctgctgcg ttaaacaaca acatggcaac
     1501 cccgtacgtc ttccaatcca tctcctcatt gggatggaca ccacacagcc gatagatccc
     1561 ccgctcgacg aaaccgagca cccgctccaa tccgcacgat tgtccctggt agactctggc
     1621 catgtaccag ccgagcggct tgaccagtgc caagaggacg aagaagaaaa gaccgatttg
     1681 tacgagtccg ttcatcgcca tcaaaaccac tccgctttga gcaacgcaat catgaggtac
     1741 acgagcaagg cgaacgacag aatccccgca agcacgtaca tgagattcat cggaccacct
     1801 ttacaaccgg tccagtgccg aaatcagcca gaacgaaagg agaaaaaagc ctgcgcccaa
     1861 cacaatcatg agcatgtcca tgcgagcatc ctcctcttcg cccagtcgcc actctagcaa
     1921 agggcccgta aaaacggtgt caaaattcct gggaccgtgg ccgtcctgct gacacgagac
     1981 tccgatcagg tggctttgga atgtgttctc cctcgtgatg caccctgccg catcctcttc
     2041 agctcctgca gggcacgttt caaatcctgc ttggaccgcg gttgacgagg caatttggct
     2101 ggatgggtca gccgctgttc aactgttgaa tcaactccgg caaaccagtc tcgcttgcgg
     2161 gtcacaagat caattttgct gcaagccatc atgtggtgct ccgtcatggt gagtcaaagg
     2221 tccagatcgc ggcgaagatc aacatgtgct gcgccgcggt caatccgact acccccggtg
     2281 aaacttggcc gtctttgaag aaccctccac ccggtccagt ggactcgtcg tatcggtatt
     2341 cgagcctgag aatcatgttg gcccacttat agggaagctt gtactccgtg gtggtggtca
     2401 ccgctcgtat gagttgctcg gatccggtca tcagaccgtt tcgatcccag tacacttccg
     2461 gtctgagcgc cacgctccaa ggacctgaga tgtgccatcg catcggcaat gcagctccca
     2521 tatagaaagc ccgtggattc cccgtttgat ccgccatctc ctgggtcccg acttgatact
     2581 cacccgcaat ggtcacgtcg tcgttgactt tccactcaac gatactgtca gaaaacaacc
     2641 gccaaaattc cagcgacgtg ttcgactgat cgggaccgta gtaaaccgtt tccttgatgg
     2701 tccaagcctg attcggcttg taagccactt ggcctccata gctgggaagg ctattcgcgt
     2761 tttgaaggtg gaagtactcg ttgatgataa agaaggtgcc cgtccatttc tcattgaact
     2821 gatacacggc attcgctccg aacatcaaat agggggaata atctgcgatc caggcacgag
     2881 tgtagttgaa gttatccttg gcatagagtg attcgtagcc gatgaagctg ttgaacaaac
     2941 cgacttgaag catcaacccg ttaccgaccg gcgcaaggta agagacgttc gcacgcccga
     3001 attgacgcaa tgtatcggac ccctgcacct tgggaaggtt cacgccgaac ccaaagtctt
     3061 tggcgtcctg gcctccctgc cccatgagtt ccatccccca gcgtgactgc tccgtcgcat
     3121 ccttccggac atagacccca cccatgttga cgtcgagttc attaacgcgc ggcgtggtgc
     3181 tgcggccgcg aaactgatgg ttctcaggaa agttgaaatc gagcgagtac cctaggtcta
     3241 gaaacccgcc atagtgccac tcgggctgtc gggacttaga gtcttcggat gacgcaggca
     3301 ccccatcgcc ggactgatgg tcggtctggc ccactggttc ctccgcaagc gacggggtcg
     3361 ccgcagccca cagtgcgagc atcacgactc tcgttatcca catcacaatc tgtcccatga
     3421 tcgcgtcctt ttccggttct tccttttcgt cgattcctca agctgccgtt gtattgttag
     3481 gcaatctatc tacgacgggc cccccgcgga tccctgaaac cccgccggtt catatgacga
     3541 tcccctctga cgatggtaat gaccgtcatc cacagcaggc ccaagcagcc cagcagcaaa
     3601 cctattgcaa ttagtgtcat gggcgactcc gtgaattgga actcgcattc ctgtactgca
     3661 gaacctatca ggaacggtat caaagccgcg taaaagcgag actggtgact atcaggacgg
     3721 tgtaaaagtg cctggggcgt tcagggaatt ccggccagat cgagcatgcg gatgaatcgg
     3781 cggcaaaaga gaatggaaac tgctcttatt gtgagccttc aggtcatagg cgaatgaggt
     3841 gctaggactt cttactgctt acgtgaccaa acagagtgag aaccgaagca tcaaggcggt
     3901 gcagccctgt tttacttggc ggactctccg aggaggcacc acatgcggcg cgtgccggat
     3961 ttctgatcgg tcaccggctc accgctaaag aagctgattt gccaggcgac gatatcgtcg
     4021 gtcggatgcg gtgtcgcagt ccaatagatt tcggacttga tgttggagaa gggatgtccc
     4081 gccggcaacg cgggatcatg ttgagatgag tcgatcaagg tcttgagttc gtcgaccgag
     4141 ggggcgcgcc agccggattt tcctccgatc gttttcgtcg cgcagcgggc cacagaggca
     4201 ctccatacat cgtggacacg atccggctct tgttcccaga cgagaccggt ctggttgtcc
     4261 ttcacgccag tcccgtccaa gaccaatgta aatcgagtcg gagagtcggc tcctgccacg
     4321 actccgtaca acgcaacgta agtgacaccc gccgcgagca caagagctct agctatgctt
     4381 gccatcgtcg ccctcctcgt caggaagatg gccgtatcct aagagagtcg cacagacggc
     4441 gcaagcggcg actgttcagg agaagagccg gcggtgttgt cggaatatga ggagtccgcc
     4501 ccaaagaaac gacgccacca tgagcaggat gccaacgttg tagcacaaat gcgcgatgtg
     4561 atgatcgtac gggagccagc cgagcagact caggatattc tcccaatcgt gtccgtccgt
     4621 ttccttgcct gtgacgccac ccaacaacat cagatccagc gctctagcat cgttgacgta
     4681 cggagcgatg tccatgaaat tttccgccgt ccaccagaga gccaccgagg ccccgaacgg
     4741 atcgcgagtc ttgatgagaa aggtcgcgag acagacgatc ggcatgagga tctgacccag
     4801 cgtcccgccg agaaacatca tgaaccggcc aaagggcatg aagaggaggt ggccggcttc
     4861 gtggaacggc aaattgatga ggtgaagaaa cgactccccc gtgtagttcg tttcaagggg
     4921 tgtcgtgatg aacctccagc cccaccacaa tagtacaagc aagatcagac tacggccggc
     4981 gaaggtcagt gaatcgtctt tctgatccgc ctcaacaaac cactcattga agagtatgac
     5041 ccacggcgaa cgggtcggca tcgacgcttg atgctgaacc tgaacattcg tctctatcgg
     5101 tcggtacttg gcaaacacaa tcccgcactt cggacagtcc actgccccac cctgttgctc
     5161 aaactgacac ttggggcaaa caactgtcgc tgtcgccggc gatgccatac ctcaccctac
     5221 gaaggaaggt aagattgggc aaggatttgc gaagaaactt gcactggatt caagcgctta
     5281 cattcaatgg agggaaaatg gattaggagt gaaagttagc a
//
LOCUS       DATDNW010000048         5387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_551334, whole genome shotgun sequence.
ACCESSION   DATDNW010000048 DATDNW010000000
VERSION     DATDNW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551334"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..733)
                     /locus_tag="VI359_01265"
     CDS             complement(95..733)
                     /locus_tag="VI359_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6972908.1"
                     /translation="MPTLIRKTFSVPPLGCNCSILGDPVTKQAIVIDPGGAPERILHE
                     VRQLGLTVTHVVHTHAHFDHFLASGEIKKTTGATLCLHPDDLELWKMLEVQCRMFGVP
                     YVSVPLPEHWLADEEKLRVGGIEAIALHTPGHTPGSLSLYVPGEQLVLAGDTLFRGSI
                     GRTDLWGGDFDAIEQSIRERLYNLADTTVVVTGHGPETAIGLEKETNQFVRA"
     gene            complement(788..1441)
                     /locus_tag="VI359_01270"
     CDS             complement(788..1441)
                     /locus_tag="VI359_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972909.1"
                     /translation="MRQSVQAAGWSALVLCLILGDGWALAQLQEPAVVPVEDYALYDR
                     VVTSKFLTNETRLVFIERLTVSRLYPDQEVPTTISLFDEHDLFDRRLPPDLVREFVFK
                     NRQPARLGAQFNFGVRYRFVTPEGTEEPEVLLAIPAIWPPVGLTQDPSLLGRLVFSRV
                     AYTLRSDQALLYVEQHRPDGTGAGFLVWFGRQAAAWSIIDTEVLWSIRPPERASESQ"
     gene            1571..2287
                     /locus_tag="VI359_01275"
     CDS             1571..2287
                     /locus_tag="VI359_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972910.1"
                     /translation="MAIQTWSVVCAGMLLLAIGCKQDESPFKAENDTLRKQLAKQESV
                     VTSLQDGNKVMQQQIDLLNQELRDAKKAVDSTKAEAKAALDSMEGQLNQAKRLSIEAQ
                     KTAAIQAAQNIQVEGKGAQADDIPRPLATVAKVVEEALGRNGYQLRVSIKTEQKAVYV
                     TDRKISNPTSLEVAGFRNQYVISIYALPANVTRLVVKADFEKLAQGGKILSVSAEEVT
                     EIERRLIAEVNKALAASSKT"
     gene            2287..3201
                     /locus_tag="VI359_01280"
     CDS             2287..3201
                     /locus_tag="VI359_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6972911.1"
                     /translation="MRPVIGFILVTTLWLVASFVPLAAIAASKPSTELARHLTSIASL
                     ATSSPMQTIPAGEFVLGSKRIDDDPYGLRTQFDDTELPQSRVWLDAYAMDRDEVSLGE
                     YLKFLQQRKQHPPDELQKLIWHVITVHSVSDQTLTRWPALYVTWLEAKDLCQARGKRL
                     PTEAEWEKAARGTDGRLFPWGEEAPESRLAMFGQHHVHEIPILAAVDSHDDGRSPYGL
                     HHMAGNVAEWVQDWFGFDYYAYMPERNPPGPTSGRYKSVRGGSWKSNPIMLRTATRGG
                     SLPDQRSATIGFRCAQSGPADATSPGVK"
     gene            3268..3957
                     /locus_tag="VI359_01285"
     CDS             3268..3957
                     /locus_tag="VI359_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY6972912.1"
                     /translation="MKTPLIMPRIVFIGFIIAIVTLVGCTSTAPVQRQSQKLEGARCC
                     QRVEATPRRASIVRTAARLVGARTIEVNGRRIAYDCAGVTRAIYLEHGIDLYDGGASD
                     DRANGVRLIYAHVRQRGKLHQGPVVYPGDLVFFDNTWDFNGDGLLNDPLTHVGVVEEV
                     ESDGTVVFISRVADAIERYRMNLALPHVHQTAEGRVLNDYIRRKRATDPKEISQLTGE
                     LFAFYGTRLDR"
     gene            complement(3961..4332)
                     /locus_tag="VI359_01290"
     CDS             complement(3961..4332)
                     /locus_tag="VI359_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6972913.1"
                     /translation="MRAIQRFGAQLQRLRVSRDLTQDQLAVKAGLSRIYLSKLELGQH
                     DPALSTLVRLAKVLRVSVTELLGETMSHSVWWQVGESRFPTRAEAEEDAQASGEMFVA
                     RFQQTKAKGLARRLLPVREKK"
     gene            complement(4516..5154)
                     /locus_tag="VI359_01295"
     CDS             complement(4516..5154)
                     /locus_tag="VI359_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972914.1"
                     /translation="MKPEQLGLSIGIAALLGAIFLIDVRTGLGFTPWLLYVIPLGLTY
                     WASYLYAPLATAVACTALVFVGYTLSPPLVPKSIALTNRIFGAITFLALGVLIVAYKL
                     LAQRLTQLTDQLRRELLERTQDLGRAVRVFRAEAAQRSGLQRDLSEASEEFTRHLTDV
                     LVAESRRLQEHVEHLEQANLAASEEDRLDHTRNELERLGKQLEQLQRDLLRQ"
     gene            complement(5155..>5387)
                     /locus_tag="VI359_01300"
     CDS             complement(5155..>5387)
                     /locus_tag="VI359_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972915.1"
                     /translation="ALGWKDTAPSVIRRMSDLQTTTRPTCVLGTPACPQCKDTGLESN
                     GGFLTCATCGLAITRQALLREALGLQLSVEPQ"
BASE COUNT         1185 a         1660 c         1549 g          993 t
ORIGIN      
        1 ccgaccggtt catcggacac taggacacca aggagcagcg cccccacgca caggagatga
       61 cggcacatgt acatagtccc ctcctcttgc cttgtcaggc ccgcacgaat tgattggtct
      121 ctttttcaag cccgatcgcg gtttcaggcc catgcccagt gaccacgacc gtcgtgtcgg
      181 cgagattata gagccgctct cgaatggatt gctcgatggc gtcgaagtcg ccaccccaga
      241 gatccgtccg accgatgctg ccgcgaaaca gcgtgtcacc ggccaagacc aactgctcgc
      301 ccggcacata gagactcagt gacccgggtg tgtgtcctgg tgtgtgcagg gcgatcgcct
      361 cgatcccacc gacgcgcagc ttctcttcgt cggccagcca atgctcgggc aacggcaccg
      421 agacgtacgg aaccccgaac atccgacatt gcacctcgag catcttccag agttccaggt
      481 cgtccggatg caggcagaga gtcgctcccg tcgttttctt tatctcgcct gaggcaagaa
      541 agtgatcaaa atgagcatgc gtatgcacga cgtgtgtgac ggtcaaaccc agctgccgca
      601 cttcatgcag aatccgctct ggtgctccgc cgggatcgat cacgatcgcc tgtttggtca
      661 cgggatcacc caggatcgaa caattgcatc ccaagggagg aacggagaac gttttacgaa
      721 tcagcgtcgg cacggctacg ctgtcctatg tgatcgaggt acgcgtcctc atgacgctcg
      781 ctcaaaatca ttgagactcc gaagctctct ccggtggacg aatgctccac aacacctcgg
      841 tgtcgatgat ggaccaggcg gccgcttgcc gcccgaacca tacgaggaac cccgcaccgg
      901 tcccgtcggg ccgatgctgc tcaacataga gcaaagcctg atccgaccgc agggtgtagg
      961 ccacgcgaga aaacaccagt cggccgagga gcgaggggtc ctgtgtcaaa ccaaccggcg
     1021 gccagatcgc agggatggcc agcaacacct ccggttcctc ggttccctcc ggagtgacga
     1081 agcgatagcg cacgccaaaa ttgaactgtg cgcctaatcg agccggctga cgattcttga
     1141 agacgaattc ccgcaccaga tcaggcggga gcctccgatc gaacaggtca tgctcatcaa
     1201 acaggctgat cgtcgtggga acctcttgat ccggatacaa ccgcgaaacg gtcaaccgct
     1261 cgatgaacac gagtctcgtc tcgttcgtca gaaacttgct cgtcaccact cgatcgtaca
     1321 aagcatagtc ctcaaccgga acaacggccg gttcttgcaa ttgggccagg gcccatccgt
     1381 cgccgaggat caggcacagg actagtgccg accagcctgc tgcttggacg ctctgtcgca
     1441 tagcgtcagc atacctgctc gaactttttc aggtcaaacc ggtgtgactg cacaatcttc
     1501 atagacaagt gtgagcgaag catggataat cggcccacct tcgcatgaat cgatggagat
     1561 gagctaaccg atggcgatac aaacgtggtc cgtggtgtgt gccggcatgt tgcttttggc
     1621 aatcgggtgc aaacaggacg agtccccctt taaggcagag aacgacacgc tccgtaagca
     1681 gctcgccaaa caggaatccg tggtcacctc gttgcaagac ggcaacaaag tgatgcaaca
     1741 acagatcgac ctgttgaatc aggaattacg cgacgccaag aaggccgtgg acagcacgaa
     1801 ggccgaggca aaggcggcgc tcgattccat ggaaggccag ctgaaccagg ccaagcgact
     1861 cagcatcgag gcgcaaaaga ctgccgcgat tcaggcagct cagaatatac aggtcgaggg
     1921 aaaaggcgcg caggccgacg acattccccg ccccctcgcc accgtcgcca aagtcgtcga
     1981 ggaggccctc ggccgcaatg ggtaccagct gagggtcagc atcaaaaccg agcagaaggc
     2041 ggtctacgtg acggatcgca agatttccaa cccgacatcg ctcgaagtcg ccggcttccg
     2101 caaccagtat gtcatctcca tttatgcgct tcccgcgaac gttaccaggc tagtcgtcaa
     2161 agcggatttc gagaagctcg cgcaaggcgg gaagatcctg agcgtcagcg ccgaagaagt
     2221 cactgagatt gagcgccgtc tgatcgccga agtcaacaaa gccctcgccg catccagcaa
     2281 aacctaatgc ggcccgtcat cggcttcatc ctcgtgacga ccctgtggct cgtcgcctcg
     2341 ttcgtccctc tcgcagccat cgcagcgtcg aagccctcga cagagttagc ccgccatctc
     2401 acatcgattg cctcgctcgc gacctcgtcg cccatgcaga caatccccgc cggcgagttt
     2461 gtcctcggca gcaagcgaat cgacgacgac ccctatggat tgcggaccca gttcgacgat
     2521 accgaactcc ctcagagtcg ggtctggctg gatgcctatg cgatggatcg cgacgaagtg
     2581 agtctcggtg agtacctgaa attcctccaa cagcgaaaac aacacccgcc tgacgaactc
     2641 cagaaactca tctggcacgt catcacggtg cactccgtct ccgaccagac cctgacacgc
     2701 tggccggcgc tctacgtcac atggcttgag gccaaggatc tctgccaggc acgagggaag
     2761 aggctgccga cggaagcgga atgggagaag gccgctcggg ggaccgacgg tcgcctgttt
     2821 ccctggggag aggaggcgcc ggagtccagg ctggccatgt tcggacaaca ccacgttcac
     2881 gagatcccga ttctcgcagc ggtggattca cacgatgacg gcagaagccc ctacggcctc
     2941 catcacatgg ctggcaatgt cgcagaatgg gtgcaagatt ggttcggctt cgattattac
     3001 gcctacatgc ctgaacggaa tcctccaggc ccgaccagcg ggcgctacaa aagcgtccgc
     3061 ggcggctcat ggaagagcaa ccccatcatg ctccgcactg ccacgcgagg cggctcgctg
     3121 ccggaccaac gatccgccac aatcgggttt cgctgcgcgc aatccggtcc cgccgacgcc
     3181 acatcccccg gcgtgaaata gccgtttgct tggtcagtcc catccctcgt gatagcctat
     3241 cctctgttta gccgcattat cgagaacatg aagacgccgc tcatcatgcc ccgtatcgtt
     3301 tttatcgggt tcatcatcgc catcgtcacg ttggtcgggt gtaccagcac cgcgccggta
     3361 caacggcaat ctcaaaaact cgaaggagcg cgctgctgtc agcgcgtgga agcgacaccg
     3421 cgtcgggcgt caattgtccg cacagctgcg cgactggtgg gcgcacgaac gatcgaggtg
     3481 aacggaaggc gcattgcgta cgattgcgcg ggcgtgacca gggcgattta tctcgaacac
     3541 ggcatcgatc tctacgacgg cggggccagt gatgacagag cgaacggagt ccgactcatc
     3601 tatgcccacg tgaggcagcg tgggaaactc catcaagggc cggtcgttta tcccggtgat
     3661 cttgtgtttt ttgacaacac ctgggacttc aacggagatg gcttactcaa cgatcccctc
     3721 acccacgtag gtgtggtgga ggaggtcgaa tcggacggca cggtcgtttt catcagtcgc
     3781 gtcgctgacg ccattgaacg ctatcggatg aatctcgccc tgcctcatgt ccatcaaacc
     3841 gccgagggcc gggtcctgaa tgattacatc cggcgcaagc gcgccactga tcccaaggaa
     3901 atcagccagc tgaccgggga actctttgcc ttctacggaa cccgcctgga tcgatagttg
     3961 ttacttcttc tcgcgcacag ggagcagacg gcgcgcaagg cccttcgcct tggtctgttg
     4021 aaatcgtgcg acgaacatct cgcccgaggc ttgtgcgtcc tcctcagcct ccgcgcgtgt
     4081 cgggaagcga ctctcgccca cctgccacca cacgctgtgg ctcatggtct ccccgagcaa
     4141 ctctgtgaca gagacccgta acaccttcgc caatcgcacc aacgtgctga gcgccgggtc
     4201 gtgttgcccc aactccagct tggagaggta gatacgggag aggcccgcct tcacggccag
     4261 ctggtcctgc gtgaggtccc gactgacgcg gaggcgttgg agttgtgcgc cgaaacgctg
     4321 gattgcacgc atgatgtgcc tcgaaacgat gggaggagaa aacgagggaa gtctacacct
     4381 cttcctatgg gcgacggaag gtggtggagg ccacctctga gacctggtcg tagccggcgg
     4441 gtgcggccta agaatcagcg gcgctgtgcg gtatcgtgcc gagatgggga gagcaccgct
     4501 tcgaaagacg cgagcttact ggcgcagcaa gtcgcgttgc aactgttcca actgcttgcc
     4561 cagccgttcc agttcgttgc gggtgtggtc caggcggtct tcctccgacg cagccagatt
     4621 cgcctgttca agatgctcga cgtgttcttg taaccgccgg ctctcagcca ccagcacatc
     4681 cgtaagatga cgggtgaact cctcgctggc ctccgacaga tctcgttgca gcccgctcct
     4741 ctgcgccgct tctgcccgga agactctcac ggctcgaccg aggtcctggg tccgctccag
     4801 aagttcccgt ctgagctggt cggtgagttg ggtcaggcgt tgcgccagaa gcttgtaggc
     4861 cacaatcaag acgcccaacg ccaaaaaggt gatcgcacca aagatccgat tggtcaaggc
     4921 aatcgatttc ggaaccaacg ggggggatag tgtatagccg acaaatacca gtgccgtgca
     4981 ggcgacggcc gtggcaagag gggcatacag gtaagaagcc cagtacgtca gtccaagagg
     5041 aatcacatag agcagccacg gtgtgaaccc caaacctgtt cggacatcaa tcaggaaaat
     5101 ggcgccgagc agagccgcaa tgccgataga cagacccagc tgttcgggtt tcatctactg
     5161 gggctcaacc gagagttgca gaccgagcgc ctcgcgcagg agggcttgcc gggtaattgc
     5221 caggccgcaa gtcgcacagg tcaagaatcc cccgttcgat tccaaacccg tgtccttgca
     5281 ctgaggacag gccggcgtcc cgagtacgca agtggggcga gtggtcgtct gcagatcaga
     5341 catacgacgt ataactgaag gagctgtgtc tttccaaccg agcgcgg
//
LOCUS       DATDNW010000049         2457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1302695, whole genome shotgun sequence.
ACCESSION   DATDNW010000049 DATDNW010000000
VERSION     DATDNW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302695"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VI359_01305"
     CDS             complement(<1..231)
                     /locus_tag="VI359_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY6972916.1"
                     /translation="MEAGRRSDLGIASGYRIKGETGSVKAKIAVLAGDGVGREIVPEA
                     VKVLKVVAEKFGHSFEFAPGDVGGQAIDKVGVP"
     gene            complement(198..560)
                     /locus_tag="VI359_01310"
     CDS             complement(198..560)
                     /locus_tag="VI359_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6972917.1"
                     /translation="MFKVTPLERPVFLAGDHTRLREIFHPAKHQLKLGYSLAHGTLGP
                     GQRSKRHVLASSEVYYFIAGQGRFMIDDQVLSVEAGTTLYVPPGGTQSLENTGAIDIE
                     FLCLVDPAWKLEDEVILE"
     gene            complement(630..2177)
                     /locus_tag="VI359_01315"
     CDS             complement(630..2177)
                     /locus_tag="VI359_01315"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY6972918.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKIMVAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIAQEVEGPTICSLARARPEDVDRAWDALKGAPKVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQRARGYVDDVEFSPMDASRSDPAYLYEVIEAVIA
                     AGAGTVNIPDTVGYAIPQEFGQLIRGIRDKVPNSAKAVISVHCHNDLGLAVANSLAAV
                     LEGAGQIECTINGIGERAGNTSLEEVVMGLRTRKDLYHADTKVRSEEISKTSRLVSKI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESIGLVESKMVMGKLSGR
                     HAFRQRLEELGYKLSDEEINHAFERFKKLADQKKEIYEEDIEVIVSEELAKMAERMVL
                     KSFHVESGTDRVPTAMVELEIDGRSVKQTGTGDGPVDAVYRTIAAMTQTKSKLLMYAV
                     KAITGGTDAQGEVSVRVQEDGRTVSGHGADTDIIAASARAYLNALNKLAYLAAKQAQG
                     EQKVNLI"
BASE COUNT          499 a          804 c          587 g          567 t
ORIGIN      
        1 cggaacaccg accttgtcga tggcttgccc tccaacatcg cccggtgcaa attcaaagct
       61 gtggccaaat ttctcagcca caacctttaa aaccttgacg gcttcgggaa cgatctctcg
      121 tccgactccg tcccctgcca acactgcaat tttggctttc acagatcctg tctccccctt
      181 gattctatag ccgctcgcta ttccaagatc acttcgtctt ccagcttcca cgcaggatcg
      241 acgaggcaga gaaactcgat atcaatggct cccgtgttct ccagtgactg tgtccctcca
      301 ggcggcacat acagcgtcgt tcccgcttca accgaaagga cttgatcgtc gatcatgaat
      361 cgcccttgcc cggcaatgaa atagtagacc tccgaagaag ccaagacgtg ccgcttggac
      421 cgctgacctg gtcccaacgt cccatgggca aggctatagc ccagttttaa ctggtgctta
      481 gctggatgga agatttcccg aagacgtgta tggtcgccgg ccagaaacac tgggcgctct
      541 aatggtgtga ccttgaacac gagtgcgccc tctcacctct gcagtagcac tgcccgaatt
      601 attcggtcac tctaccgtgg cgcaacttct caaatcaagt tcaccttctg ctcgccctgg
      661 gcttgcttcg ccgccaaata cgccagtttg ttcaacgcgt tgagataggc ccgtgccgat
      721 gctgcgatga tgtccgtatc ggccccgtgg ccggataccg tgcgcccatc ctcttggacc
      781 cgcaccgaaa cctcaccttg ggcatcggtc ccccccgtta tcgccttcac cgcatacatg
      841 aggagcttgc tctttgtctg agtcatcgct gcaatggtgc gatacaccgc gtcgaccggt
      901 ccatcgcctg tcccggtttg cttcacagac cgcccgtcta tttcgagttc gaccatcgca
      961 gtcggcacgc gatccgtacc gctctcgacg tggaacgatt tcaggaccat ccgctccgcc
     1021 atcttcgcca actcttcaga aacaatgacc tcaatatctt cctcgtaaat ctctttcttc
     1081 tgatcggcca acttcttgaa ccgttcgaat gcgtggttga tctcctcatc cgacaatttg
     1141 tatcccagct cttcaagtcg ctgtctgaac gcatgtcgcc cggaaagctt gcccatcacc
     1201 atcttgctct cgaccaaccc gatcgattcc ggccgcataa tttcataggt cgttttgtcc
     1261 ttcagcagcc catcctggtg aatacccgac gtatgcgcaa aggcattcgc acccacgatc
     1321 gccttgttgg gctgcaccac catcccggtg atcttgctca cgagccggct ggtcttggag
     1381 atctcttccg agcggacctt cgtgtcggca tggtagaggt cctttctggt ccggagtccc
     1441 atgacgactt cctccaacga tgtattcccg gcccgttcgc cgatcccatt gatcgtgcat
     1501 tctatttgac cggctccctc gagcacagct gccaaactgt tcgcgacagc caatcccaag
     1561 tcgttgtggc aatgaaccga tatcacagcc ttcgcactat ttggcacctt gtcacgaata
     1621 cctcgaatca gctggccgaa ttcctgcgga atcgcatatc cgacggtgtc tggaatattg
     1681 acggtgcccg ctccggcagc gatcaccgcc tcgatgacct catagagata ggccggatcg
     1741 gaccggctgg catccatcgg cgagaactcg acatcgtcga catagcctct cgcccgctgc
     1801 accatctcaa ccgcgcgttt cttggcttcc tcccgcgtca tacgaaactg gtgtttcaaa
     1861 tggatgtccg acgtggaaag gaaggtgtga atgcggactt tgggcgcccc tttgagcgcg
     1921 tcccacgctc gatcaacatc ctccggacgg gcccgtgcca agctgcagat cgtcggtcct
     1981 tccacctcct gtgcgatccg ccgcacagcc tcgaagtcac cgggagaact ataggcaaac
     2041 ccggcttcga taatgtcgac gccgagacgg gccagttgct tcgccaccat gattttttct
     2101 tccacattca tgctggcacc aggagattgc tctccatccc gcaacgtcgt atcgaaaatt
     2161 ctgatcatgc gtgtcatggc atcacccctc tccgattcgg actgctcaaa aagtccgaca
     2221 ttctcacccg cccgaccctg gcgcgccgag acgcgccctt tcccaggcaa ggcggcaggc
     2281 gcagaaaata ccccaggcgt accctctggg atatgttgag ggtgctttca agccgagaac
     2341 gaagctggtg gccggtttga gcagtctgca aaaacacaaa agcctccatc ccagaagaca
     2401 gggacgaagg cttcacgcac tccgtggtac caccctggtt cggcgatgga cggccac
//
LOCUS       DATDNW010000050         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_141978, whole genome shotgun sequence.
ACCESSION   DATDNW010000050 DATDNW010000000
VERSION     DATDNW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141978"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..972
                     /locus_tag="VI359_01320"
     CDS             151..972
                     /locus_tag="VI359_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY6972919.1"
                     /translation="MALRFLFLFSIGLALHGTALPLVFAEVSGNLVIAGNGPELTTIE
                     PLARAFEKANPRAYVDVLWDENSKPVEMVKSGQAQFAVTGADDSGLMSTQIAWDGIGV
                     MVHLSNFTKEVTKQEVADIFSGKVKMWSELGGPETKILVIDRPRNQNIREAFESQLGI
                     TGKITDSAKVIGRDDKVIKTVVGTLPPLSAVSYLSMSQGLSVVSGGVAVRLLPIDKVE
                     PEIPTVKDGRYPLRRPVLLLTKKDLHPVAEAFKQFALSPAGQKIIAETYVPVGEK"
     gene            1001..1459
                     /locus_tag="VI359_01325"
     CDS             1001..1459
                     /locus_tag="VI359_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY6972920.1"
                     /translation="MCTWRSSALIVIGLAGWLLLPMTTLAADQEGSMVEGTGFSLFDT
                     ESIKGFDAEKVQRDPTCDRTKRPRIAKVEPDEAKPGDKVVITGENFGTKECFHTVTFS
                     AASKTPVDFKYVSDTSIEATVPEAKAGMSFIIIVAGGGSAQSKPLLIKSK"
     gene            1643..>3050
                     /gene="accC"
                     /locus_tag="VI359_01330"
     CDS             1643..>3050
                     /gene="accC"
                     /locus_tag="VI359_01330"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY6972921.1"
                     /translation="MFKKILVANRGEIAMRIIRACRELNIATAAIYSEADSTGIYVKK
                     ADEAYAVGPGPVKGFLDTQQIVDLAQRIGADAIHPGYGFLSENAQFAQLCQTSGITFI
                     GPSPRAIELMGNKVKARELAKQVGVPIVPGTENGVSHVKDALAFTQQAGYPVMIKASA
                     GGGGRGLRVVRSDAELRENIEVAGREAQAAFGDSSVFIEKYIERPHHIEFQILADRHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLILTSRLRAEMGEAAIAIARGVQYDNAGTVEFL
                     LDQEGRYYFIEMNPRIQVEHTVTEQITAIDIVRNQIAIAAGKPLDIRQEDVTLQGHAI
                     QCRINAEDPKNNFRPCTGTVTAYLSPGGIGVRIDGAVYKDYIIPPYYDALLAKLTVRG
                     RTWEETVSRMRRSLEEYVLRGVKTTIPFMKTIMQDPDFLAGRFDTSYLETHPELFNYD
                     EFEQPEDLVLAISAAIAAY"
BASE COUNT          677 a          905 c          841 g          627 t
ORIGIN      
        1 tgcccctcca ccgatggacg ccgtggcctc aggttcccct ccaatcgacc agacaaagcc
       61 gtcagaatcc tgaaccgcgc cgcaggctgc ccgttgaacc cctcgtctca ccatcgccgt
      121 gatgtccgaa tcgacaagcg aggtgcacgc atggccctgc ggttcttatt cctcttcagt
      181 ataggtctcg ccctccatgg aaccgctctt cccctcgtct tcgccgaagt ctccgggaat
      241 ctcgtcatcg cgggcaatgg gccggaattg acgaccatcg aaccactggc tcgcgcgttc
      301 gagaaagcga acccgcgagc ttacgtcgac gtgctctggg atgagaattc caagcccgtc
      361 gagatggtca agtccgggca ggctcagttt gccgtcactg gcgccgatga ttccggatta
      421 atgtcgactc agattgcatg ggacgggatt ggcgtgatgg tgcatctctc gaatttcacc
      481 aaagaggtga cgaaacagga agtggccgat attttttccg gcaaggtcaa aatgtggtct
      541 gagctggggg ggccggagac gaaaatcttg gtgatcgatc gtccccgcaa tcagaatatc
      601 cgcgaggcct tcgaatcgca gctcggtatt accggaaaaa tcacggattc ggccaaagtc
      661 atcggccgag acgacaaggt aatcaagacc gtcgtcggca cacttccccc gctctcagcc
      721 gtctcgtacc tctcgatgag ccagggcttg tccgtagtct cgggaggtgt tgcggtgcga
      781 ttgcttccga ttgataaggt ggagccggag attccgaccg tgaaggacgg ccgctatccc
      841 ctaagacgac cggtcctgct gctgaccaag aaagaccttc acccggtggc cgaggccttc
      901 aaacaattcg cactttcacc ggctggccaa aagattattg ccgagactta tgtgcccgtt
      961 ggtgagaaat aatcgttcgt tcttactcaa ggaggttcat atgtgcacat ggcgatcatc
     1021 agccctcata gtcattggac tcgccggctg gctcctcctg cccatgacca ccctcgcagc
     1081 cgatcaggaa ggttcgatgg tcgaaggaac cggtttctct cttttcgata ccgaatccat
     1141 caagggattc gatgccgaaa aagtccagcg agacccgacc tgcgaccgca ccaagcggcc
     1201 gcggattgcc aaagtggagc cggacgaggc caaaccaggc gacaaggtgg tcattacggg
     1261 ggaaaatttc ggtacgaagg aatgtttcca taccgtgacc ttcagtgctg cctcaaaaac
     1321 gccggtggac ttcaagtatg tcagcgacac ctcgattgaa gccacggttc ctgaggcgaa
     1381 agccggcatg tcgttcatca tcatcgtcgc cggtggcgga agtgcccagt ctaagcctct
     1441 cttgatcaag tccaagtaag attctccaga ggctcggagg cattctcgca ttccgagcct
     1501 catgcctttc ctcacgccgc gagtttcggc gcaacccccc gtgttctctc gacaactgtc
     1561 tcggtcctat gctagcgtac gcccgctttt gcaccgttcc ccatttgtgg aatggcgaat
     1621 ggattctgag gtaagaccgc gtatgttcaa gaagattttg gttgccaatc gcggcgaaat
     1681 cgcaatgcgg attatccgag cctgccgcga actcaatatt gcgaccgcag ccatttactc
     1741 cgaagccgac tcgaccggta tttatgtcaa gaaagccgac gaggcctatg cggttggccc
     1801 gggaccggtc aagggtttct tggacaccca acagatcgtg gatcttgccc agcgcatcgg
     1861 agccgatgcg atccatccgg gatacggttt cctttcggaa aacgcgcagt tcgctcagct
     1921 ctgccaaaca tccggcatca cgtttatcgg tccgtctcct cgcgccatcg aactgatggg
     1981 caacaaggtc aaagcgcgtg aactggcgaa gcaagtgggt gtgccgatcg tgccgggaac
     2041 cgagaatgga gtgagccacg tcaaagacgc tcttgccttc actcaacagg ccggttatcc
     2101 cgtgatgatt aaagccagcg ccgggggcgg aggacgtggt ctccgggtcg tcaggtcaga
     2161 tgccgaactt cgcgagaaca tcgaggtcgc cgggcgagaa gcccaggccg cgttcggtga
     2221 tagtagcgtg ttcattgaga agtatatcga gcgcccccat catatcgaat tccagattct
     2281 ggccgatcga cacggcaaca ttatccacct gggtgaacgc gactgctcaa tccaacgacg
     2341 gcatcagaag ctgatcgaga tcgccccctc cctcatcctg acgtcccggc tccgcgccga
     2401 aatgggagaa gccgcgatag cgatcgcgcg cggggtacag tacgacaatg cgggaacagt
     2461 ggagtttctt ctcgatcagg agggtcgcta ctacttcatc gaaatgaacc cgcgcattca
     2521 ggtcgaacat accgtcaccg agcagattac cgcgatcgat atcgtgcgca atcagatcgc
     2581 catcgccgcc ggcaagccgc tcgatatccg gcaggaagat gtcacgctcc agggccatgc
     2641 catccaatgt cgcatcaacg ccgaggaccc gaagaataat ttccgcccct gcaccggcac
     2701 ggtcaccgct tacctctcgc caggcggcat cggcgtgcgg atcgacggag ccgtgtacaa
     2761 ggactacatc atcccgcctt actacgatgc cctcctggcg aaactcaccg tgcgaggccg
     2821 cacctgggaa gaaacggtca gccggatgag gcgatcgctg gaagaatatg tgttgcgggg
     2881 cgtaaaaacg acgattcctt tcatgaagac catcatgcag gatccggact ttctggcagg
     2941 ccgcttcgat acgtcctacc tcgaaaccca tcctgaactc ttcaactacg acgagttcga
     3001 acagccggag gatttggtac tggccatatc agcggcgatt gccgcctatg
//
LOCUS       DATDNW010000051         3840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_483959, whole genome shotgun sequence.
ACCESSION   DATDNW010000051 DATDNW010000000
VERSION     DATDNW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483959"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /gene="murJ"
                     /locus_tag="VI359_01335"
     CDS             complement(<1..1358)
                     /gene="murJ"
                     /locus_tag="VI359_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY6972922.1"
                     /translation="MADPAAGPTSSDKRDGENHLVVDAAGMIGVATFSSRILGFIRDM
                     VLAQLFGATPAADAFFVAYRIPNLLRELFAEGSMSSAFIPVFTEYQTLKSKRDAWELA
                     SAVFTTLLTIVTGITIIGIIAASGIVWLMAPGFHDEPAKLEMTTLLTRIMFPYLIFIS
                     LAALAMGILNSLRAFAVPAFSPVFFNIVIISCAYFLSPMLPEPIVGVAVGVVAGGAAQ
                     FAIQLPGLKLRGMLFGFRYEPGHPGVRRIGWLMVPSLLGLSVTQINITVSTVLSSFFA
                     GGPTYLFYGMRLIQFPLGIFGVALATAILPTLSSQAAREALDELRTTLGFGLRMILFI
                     ILPAMLGLILLRQPIVHLFFEHGSFTHDDTVMTATAVLCYAVGLWAFAGVRIIVSAFY
                     SLQDTKTPAITAAVSVGANVVFSLIFMRTLGAAGLALATALAAMLNGAILVAVLNRRL
                     GGV"
     gene            1449..1754
                     /locus_tag="VI359_01340"
     CDS             1449..1754
                     /locus_tag="VI359_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972923.1"
                     /translation="MSLERSSRLSGGFQIRHRTLGVYQGSAIELAFWHPSSGMPEYGL
                     CRFATKEKADSYVAYLSSPACPEPLDSNDLTIEPYDLAEHERLTNDHPLPSAWENPI"
     gene            complement(1834..2217)
                     /locus_tag="VI359_01345"
     CDS             complement(1834..2217)
                     /locus_tag="VI359_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6972924.1"
                     /translation="MASRIGLDLPKHPMDRREHLRIPVRLYLSFSGGKVRGEGTVLDI
                     SVGGCIIKSETQVHQDDIFYLQLTLDESEPPLEVAAMVRSVSARGIAFKFLRAAQENK
                     RLLAFVQTRTSDHHDKSPLKVGVAG"
     gene            2545..2916
                     /locus_tag="VI359_01350"
     CDS             2545..2916
                     /locus_tag="VI359_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY6972925.1"
                     /translation="MRIFETHYIRVVVLGVSVALESCDRDFQAVRITAQDFRFTPATI
                     HLTAASPIYLTIVNEGREPHEFESSILAHRVDGSGGNATSVRVASNQRTDMMIRTIPG
                     TYIFYCRIRGHANMSGTIIVE"
     gene            3098..>3840
                     /locus_tag="VI359_01355"
     CDS             3098..>3840
                     /locus_tag="VI359_01355"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY6972926.1"
                     /translation="MLWIESWDYIPNVVRPNRTQALSSATLRLLRPLARILLRNNVSH
                     QTFAELAKQVYVQIANAEFGITGKKQTISRISILTGLTRKEVQRLLTQPPDTTTAASE
                     EYHRATRVITGWVRDPDFGDGKGHPLPLRMEGKRASFSELVKRYSGDIPVRAMLDELL
                     RVGAIKQLKDGRICLVSLGYVPQKDQVEKLQVLGTDTADLIATIDHNVYQKPSKPRFQ
                     RKVMYDNVPVEAAKEFRILAAAKGQELLET"
BASE COUNT          906 a         1124 c         1078 g          732 t
ORIGIN      
        1 acacccccca atcgacgatt caacaccgcc acaagaatcg cgccgttcag catcgcagcc
       61 agtgccgtcg caagcgcgag ccccgcggcg cccaacgtgc gcatgaagat cagggagaac
      121 accacgttcg ccccgaccga caccgccgcg gtgatcgcag gcgtcttcgt atcctgtaaa
      181 gaatagaacg cggagacgat gatccgcacc ccggcaaacg cccacagacc caccgcataa
      241 cacaggacgg ccgtggcagt catcaccgtg tcatcatgag tgaaggaacc gtgctcgaaa
      301 aacagatgaa cgatcggctg gcgcagcagg atcaagccca gcatcgcggg cagaatgata
      361 aacagaatca tgcggaggcc gaaccccaag gtcgttcgca attcatccaa tgcttcgcga
      421 gccgcttgcg aagacaacgt cggcagaatc gccgtcgcca gggccacgcc aaagataccg
      481 agcgggaatt ggatcaggcg cataccgtag aacaaatagg tgggcccacc ggcgaagaag
      541 gacgagagca ccgtactcac cgtgatgttg atttgcgtca cggacaaccc caacaacgac
      601 ggtaccatga gccagccgat ccgtcgcact cctggatgac ccggctcata ccgaaatcca
      661 aacagcatcc cgcgcaactt gagcccgggc agctgtatgg cgaactgagc cgcaccgccg
      721 gccacaacac cgacggcaac accgacgatc ggctccggca gcatcggcga cagaaagtat
      781 gcacagctga tgatgacaat gttgaaaaac accggcgaaa acgccggcac cgcgaacgcc
      841 cgcagagaat tcaaaatgcc catcgccagg gcggccagac tgatgaaaat caaatatgga
      901 aacatgatgc gcgtgagcag cgtcgtcatc tccagcttcg ctggttcgtc atggaaaccg
      961 ggcgccatca accagacgat gccggaggca gcgatgatgc caatgatggt gattccggtg
     1021 acgatcgtga gcagagtcgt gaataccgca cttgccaatt cccatgcatc ccgttttgac
     1081 ttgagcgtct gatattcggt gaacaccggg ataaaggcgg aagacatcga tccttcggca
     1141 aacaattcgc gcaagaggtt cgggatgcgg taggccacga agaaggcatc ggcggcgggt
     1201 gtcgccccga agagctgtgc gagaaccatg tcgcgaatga atccgaggat gcgactggag
     1261 aatgtcgcga cgccgatcat cccggcggca tcaaccacca agtgattctc accatccctc
     1321 ttgtcggatg acgtcgggcc tgcggcggga tcagccattg acgggattct atcggaaccg
     1381 gtcaggacag tctataattc accgcgtgga atgctaagat cccggcactg attggaagcc
     1441 gcgcactcat gtctctcgaa cggtcatctc gcctgtcagg cgggtttcag attcggcatc
     1501 gcacgttggg agtttatcaa ggcagtgcga ttgagctggc attctggcat ccctcatccg
     1561 gaatgccaga atatggattg tgcagattcg ccaccaagga gaaggcagat tcctatgtcg
     1621 cgtatctctc ctctcccgcg tgccctgagc cgctcgattc caacgatttg accatcgagc
     1681 cctacgacct tgcggaacac gagcgactga ccaacgacca tccactcccc tccgcctggg
     1741 agaatcccat ctgatgtctt cacacgatgc agtgagaact ttgaacggcg agataatctg
     1801 tcgcctgact ccgtctcagt tgtgcgccga gaattagccg gcaacgccga ctttgagtgg
     1861 agatttgtcg tggtgatccg aggtgcgggt ttggacgaag gcaaggagtc gcttattttc
     1921 ctgggcggcg cggagaaact tgaatgcgat tcctcgggcg ctcacagacc ggaccattgc
     1981 ggccacttcg agcgggggct cgctctcgtc gagcgtcaac tgcagataga aaatgtcgtc
     2041 ctggtggacc tgcgtctcgc tcttgataat gcacccgccc accgagatgt ccaggaccgt
     2101 cccctctccg cgaacctttc ctcctgaaaa agacaaatag agccgcacgg gaattcggag
     2161 gtgttcgcgc cgatccatcg ggtgcttggg aaggtcaagc ccgatgcggg aggccatgaa
     2221 gcgaaagctg cactgcggac aatagaaggg ggagaccata aggaaggatg cgagacgttc
     2281 gcaaggcgat cgcgggcgag tccggagcac gctcgactgg cggcattgcg gacagttcag
     2341 aagtcggccc attcgatcag ctcctcggtg ccacatctcg gtctggcctg ccaacgcctc
     2401 gtcacacttg aactatatcg ggctttccgc ggcagtcaag ccgaacacta ccttgtgcgg
     2461 aggcgcatcc gcccagtttt tccggttgct catcctgctg cgcgacttgt cttcttctca
     2521 ggcgctcgtt acaagaagag ggtcatgcgc atcttcgaga cgcactacat ccgagtcgtg
     2581 gtgctagggg tgagcgttgc attggagagt tgcgaccggg acttccaagc ggtgcgcatc
     2641 acggcgcagg actttcggtt cactccggcc acgattcacc ttaccgccgc atcgccgatt
     2701 tacctcacga ttgtcaacga agggcgcgag ccacatgagt tcgagagttc cattctggca
     2761 caccgagtcg acgggtcggg cgggaatgca acgagcgtgc gcgtggcgtc gaatcaacgg
     2821 accgacatga tgattcggac cattcccggc acctacattt tctattgcag aatccgcggc
     2881 catgccaaca tgagcggtac gattatcgtt gagtgaaagc atgtcgaaga ctcgtcaccg
     2941 cgcctgttcc ggccaggtac cccgtcgccc aggctcactg gaagttgaat ccaccgatgc
     3001 ggccatcaca ctcgaggatt tgggcaacca agcatagctc atgtaccagc cccgccctca
     3061 ttggggataa agtcgggaac cgtctcttgg ggaactgttg ctttggattg agtcgtggga
     3121 ctatattccc aatgtcgtga gaccgaaccg tactcaggcg ttgtcttcag ccaccctccg
     3181 cctcctacgg ccgctggccc gaattctcct gcgaaacaat gtttctcatc aaacgttcgc
     3241 cgaactcgcc aaacaagtgt atgtgcagat tgccaatgct gagttcggga ttacgggcaa
     3301 gaaacaaacg atttccagga tctcgatcct caccggactg acgcgcaagg aagtgcaacg
     3361 attattgaca caaccgcccg ataccactac cgccgcgtca gaagaatatc accgagctac
     3421 tcgagtcatc acaggctggg ttcgtgaccc tgacttcggc gatggaaagg gccatcctct
     3481 accattacga atggaaggaa aacgcgcgtc gtttagcgag ttggtcaaac gctacagtgg
     3541 ggacattccg gttcgagcga tgctggacga gttgcttcgt gtcggtgcga ttaaacagct
     3601 gaaagatggg cggatttgtc tcgtctccct tgggtatgta ccgcagaaag atcaggtcga
     3661 gaaattgcag gttctcggaa ctgataccgc cgatctcatc gcgaccatcg accacaacgt
     3721 gtatcagaaa ccgtccaagc ctcgctttca acgaaaggtc atgtacgaca atgtgcccgt
     3781 cgaggccgcc aaggaatttc ggatcctcgc ggccgctaaa ggacaggagc tcttggagac
//
LOCUS       DATDNW010000052         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_73492,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000052 DATDNW010000000
VERSION     DATDNW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73492"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2290
                     /locus_tag="VI359_01360"
     CDS             <1..2290
                     /locus_tag="VI359_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY6972927.1"
                     /translation="NEDAIHASFAGLYRTHLGVPLSGMEWAIKDLWASVWDERVIQYH
                     QQSGPTEAPPAMAIVIQPMLDAQVAGVAYSVHPLTGRADAVLVNAVPGLAAPLVEGTV
                     TPDQYVLRVSPNRRTVTVAERIIVKKERALRLVAEGVREEAVLQGNAECSSLTDQQLT
                     ELARVTKKVEQALHAPVDIEWLIDERGLWLLQARPITHRRDPSLLTNDDCEWSRANFK
                     ETLPELPSPLGLSFLERFMEAYIIAPYRRLGCTIPVRVTSVRILHGRPYINMTLMHSL
                     ILQLRGNPSLVTEQMGGESLTTIPGVQPLGWYSYARAGLLSVREMGKALRYGPAWFKE
                     MKNFAVLYQPDRVRCLTNRDLSVRLDDLERWLSTHELTFGIAGGVSLCLQALGTLLPR
                     WLGEDWRALLNAALQGQGSVVSAQQIVRLAEVAEIARSEPAVFGLLTAEVPIPMDFRQ
                     SFKDTEFLRAFERYLEEYGHRGIGESDVMSPRFADQPELLLSIVRARLLSPAKAAPTD
                     ILARQASVRAQALGEIRRRCGWFLHRWLIFSWWYRCLCRFFALREASRHHLMYYSMAA
                     RNLLLRLSELLVEQGVLGSREDMFFLTLDERVAVTAGPQRDWREVVRARRMERERHRL
                     VTVPDTIRDWKDVCNGYAGHDTDLSEKALRGIPVSAGSVSGPARLVRSMADWGGVRAG
                     DIIVTSVIDPGMAPLFVIAAGLVAEMGGTLSHGAIIAREYGLPAVVNVPGATVRIREG
                     DSLMVDARRGTVLIQRETDVTP"
BASE COUNT          503 a          672 c          755 g          576 t
ORIGIN      
        1 gaatgaagat gcgatccatg ccagctttgc ggggttgtat cggacgcatc tcggtgtgcc
       61 gctgtccggg atggaatggg caatcaagga tctctgggca tcggtttggg acgagcgggt
      121 gattcagtat catcagcaat cgggaccgac cgaggcacca ccagcgatgg caatcgtcat
      181 tcaacccatg ctcgatgccc aagtcgccgg tgtggcctat tcagttcatc cgctgaccgg
      241 tcgagcagat gctgtgctgg tgaatgcggt tccaggtctc gctgcgccac tggtagaggg
      301 gacggtgacg ccagatcagt acgtcctacg agtcagccct aatagacgga ccgtcacggt
      361 cgcggaacgg atcatcgtca agaaagaacg ggccctgcgt ctcgtggctg agggtgtgcg
      421 agaagaagcc gtcttgcagg gcaatgctga gtgttccagc ctgacggatc aacagttgac
      481 tgaactggcc cgagtcacca aaaaggtcga acaggcactc cacgctccgg tcgatatcga
      541 atggctcatc gacgaacgag ggttgtggct cctgcaggca cgccccatca cccatcgacg
      601 cgatccgtca ctgctcacga acgacgactg cgagtggtcg cgtgcaaact ttaaagaaac
      661 gctgccggaa ctgccgagcc cgttagggct ctcgtttctt gaacggttca tggaagccta
      721 catcatcgcc ccgtaccgac ggctcggttg cacgattcct gttagagtga catcggtccg
      781 aattcttcac ggccgtccct acatcaacat gaccctgatg cactcgctta tcttgcaact
      841 gcgcggaaat ccctcacttg tcacggagca gatgggggga gagtcgttga cgacgattcc
      901 aggtgtgcag ccactcggat ggtattcgta cgcgcgagct ggattattga gcgtgaggga
      961 aatgggaaag gccttacgat acggtcccgc ctggtttaaa gagatgaaga acttcgctgt
     1021 cctgtaccaa ccagatcgtg ttcgatgctt aaccaacagg gatctctcag tccgtctgga
     1081 tgaccttgaa cgatggctgt ctactcatga actcacgttt ggcattgccg gcggggtgtc
     1141 tctgtgcctt caggcgctgg ggaccctcct gcctcgctgg ttaggtgaag attggcgggc
     1201 cttgctgaat gcagcacttc agggacaagg atcagtggtc agcgcccagc aaatcgtgcg
     1261 gttggcggaa gtagcggaga tcgccaggtc cgaaccggcg gtcttcggcc tgttgacagc
     1321 agaggtgccg attccaatgg acttccgaca gagtttcaag gataccgagt tccttcgagc
     1381 gttcgaacgg tatctggagg agtatggcca tcgtggcatc ggcgaatcag acgtcatgtc
     1441 tcctcgattc gcggatcaac cggagctgtt gttgtctatt gtacgtgccc ggctcctctc
     1501 tccagccaag gcagccccga ccgacattct tgctcgtcag gcttccgtgc gtgcccaagc
     1561 gttgggagag atccgacgac gatgtgggtg gttcctccac cgatggctga ttttttcctg
     1621 gtggtatcga tgcctctgtc gattctttgc cctgcgagaa gcaagccggc accatttgat
     1681 gtattactcg atggcggcca gaaatcttct gctgcggtta agtgagttgc ttgtggagca
     1741 aggtgtactc ggttcacggg aggatatgtt cttcctcacg ctcgatgagc gggtcgctgt
     1801 tacggccggc ccgcagcgtg attggcggga agttgtgcga gcccgtcgca tggagcgcga
     1861 gcgccaccgg ctagtcacgg tgccggatac gattcgagat tggaaagatg tctgcaatgg
     1921 atacgctgga cacgataccg atctgtccga aaaagcgttg cgaggcattc ccgtcagtgc
     1981 cggctctgtt tctggaccag ctcggcttgt tcggtcaatg gccgactggg gaggggtcag
     2041 ggcgggcgat atcatcgtca cgtcagtgat cgatccaggc atggcgccac tctttgttat
     2101 cgccgcgggc ctcgttgccg agatgggagg aaccttatca catggtgcca tcatcgcgcg
     2161 cgagtatggc ttgccggccg tcgtcaatgt gccaggcgcc accgttcgga tacgtgaggg
     2221 tgattctctg atggtcgatg cgaggcgtgg gactgtcctc atacaacgcg agacagacgt
     2281 cacgccatag aaatagaaga tgcgactcac cgttactatt ccgaacaaac ttgatgtagg
     2341 gccgatcggc cctatgcgtt tcccgccctt gacgttttgc gtcggtttgc gtactctcca
     2401 aaaaaatcct ttcctgatta gagtcgcact cggaacaagt ctgtcggttt ctgtctagtt
     2461 cttctgatgg cgcgagtcaa gaactatggt tgacagtact gccttg
//
LOCUS       DATDNW010000053         5014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1029742, whole genome shotgun sequence.
ACCESSION   DATDNW010000053 DATDNW010000000
VERSION     DATDNW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029742"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..276
                     /locus_tag="VI359_01365"
     CDS             10..276
                     /locus_tag="VI359_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY6972928.1"
                     /translation="MTPNSLEACIGTYECTFCAACVENVLGNVCPNCGGGFVPRPIRP
                     SKNWKGGNFLGKDPASTQIRHRPVDVAAHTLFSAAIRTTPPEKR"
     gene            complement(345..1529)
                     /locus_tag="VI359_01370"
     CDS             complement(345..1529)
                     /locus_tag="VI359_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6972929.1"
                     /translation="MTRSSLEDALEAHLTWWGLKHFTTDAEYFAWQRERLSSTDLHHL
                     NRQIERKREGDRRDEIAFYDLTAQPHILPVLYSQRYEYYSEIGSRIAAHIRGAESILD
                     FGCGVGILTTFYARQFSDKQFLGIDRSPASISVAQQQAQKRGLTNVRFECVDVECSAL
                     SGTYDLIIATHALVQAEQDPGIPSQGWQTFERARDARQQKEFEQRTAIGVRLDRLNEA
                     LTPNGRMIVFEKTRQLARRIPLERAFAMRGLRLLQKPEPIRYSLVEEVSDDGPLYVLQ
                     RGSGASFSWDESPEPDEGLPFERSNLKIVSTDPEMPLYENHWPSAQRVWEALNGKQIT
                     TETTRQEPDDRQMHVELGTAEGLSYLYCANTFDQRQLVIVEPARAAMLETYYQEIVTG
                     SG"
     gene            complement(1536..2873)
                     /locus_tag="VI359_01375"
     CDS             complement(1536..2873)
                     /locus_tag="VI359_01375"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY6972930.1"
                     /translation="MDKLLKKPRTVMKPMALLSGSIKDKALRAMADRIDTDKEKILAA
                     NEQDVEAVGKLYAAETSKDRMKAAVARVRIAADDIKEMAERLRAIADLPDPVGVVTKR
                     WEQPDGLQVSRVRVPIGVIGVISEMMPLVTVETIALCLKSGNVCVFRGAPEWGLTHQV
                     IGTGIREAAEQAGVPAGAWILVDRPEKEVAVELIRAGKNLDAIIPRGGPGLRKAVLDQ
                     AKMPVLCHDGGLTHLYVDADAELPMVQNLVVNSKVQSAGAANALDTLLVQQVIARQFL
                     PALINRLLVEFKVQVRGCSKTVALMGQMPISGHTSITPATEEDWDKQFQAPVLAVKMV
                     VNMEEALAHIAEHGPCLTAVIATARYDSAMRFAREVDASAVLVNASSRLNAADSYGAG
                     PDVGLSTSRIHAKGPIGLEQLTCEKYVVFGTGQLRLPHPIPETYFDAIMLKRP"
     gene            complement(3019..4302)
                     /locus_tag="VI359_01380"
     CDS             complement(3019..4302)
                     /locus_tag="VI359_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6972931.1"
                     /translation="MTTLRESIQSLTNAKSEEVVPLAWAFLYFFFLLCGYYILRPVRD
                     EMAIEGGVQHLPWMFTGTFVTLLVVTPLFGYLSACVPRHRLLLTVYLFFAANLIGFFV
                     VMMSHITPEWTARVFFVWLSVFNLFVVSVFWSFMADLFTPEQGARLFAVIAAGGSTGA
                     MTGPLLTAGLTFVVPVATLMLISAVFLVGCCICIHQLDRWARHHLIAPVGQQGEPIGG
                     SIWGGIRLALTSPYLMGICVYLFCLTTTATFLYLEQARLIGMQIPSPESRTRLFAIVD
                     LAVNVIAFITQAFVTGRMISALGLGSALVFLPIASVIGFSTVGLMPVLVVFILFTVVR
                     RVGEYAISKPAREVLFTVVSREEKYKAKNFIDTAISRGGDAATGWIVNGVKAFGATTT
                     QLAWVLVPISLLWGWLAWSLARHEEQRRREQVAPE"
     gene            4377..>5014
                     /locus_tag="VI359_01385"
     CDS             4377..>5014
                     /locus_tag="VI359_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972932.1"
                     /translation="MTPDDARAQYNYLMTLCIRREEAFGPMALAFLREPRFEQLGLTL
                     EEQFNLYMATAEAFAPEPKRYTAKLECLQKAQALLPRTRFFDPGLARQLQQDIQKTTT
                     DLDIYTQAMKVARPHDLTAQRLIVETEVPEYFLDLAQKRAAAYYQTKYHLPSESKTAQ
                     HFGGPPKKFEPDNPTIQKEFPGACAPFMAARTNAFHLLLPFDLKISRTPEDPL"
BASE COUNT         1124 a         1516 c         1326 g         1048 t
ORIGIN      
        1 aatacacctc tgacgcccaa ctcacttgag gcatgcatcg gcacctatga atgtacgttt
       61 tgtgccgcct gtgttgagaa tgttttgggc aatgtctgtc caaactgtgg cggcggtttc
      121 gtacccaggc ccatcaggcc atcgaagaac tggaagggtg gcaactttct tggcaaggac
      181 ccggcgagca cacaaatacg gcacaggccg gttgatgtcg cggctcacac attgttctcg
      241 gctgccatca gaactactcc gcctgagaag cggtagcgca ggaaagcgca tcatccacac
      301 gtgtctagac ccatctcgca gcgtgtggcg aggctgctcc tgattcaccc actcccagta
      361 acaatttcct ggtagtaggt ttcaagcatg gccgcgcggg ctggttcaac gatgaccaat
      421 tgccgctggt cgaaggtgtt ggcgcagtag agataagaaa gaccttcggc cgtccccagt
      481 tcaacgtgca tctggcggtc gtccggttct tggcgggtcg tttccgtcgt aatctgcttt
      541 ccgttgagcg cttcccagac acgctgggca gaaggccaat gattttcgta gaggggcatc
      601 tcaggatcag ttgacacaat cttcagattc gatcgctcga agggtagacc ttcatccggt
      661 tccggcgatt cgtcccagct gaaagatgcc ccgctccccc tctgcaacac ataaagcgga
      721 ccgtcgtccg agacttcttc aaccaatgaa taacggattg gctctggctt ctgtaatagg
      781 cgaagacccc gcatcgcgaa ggctcgttcc aacggaatgc gccgtgcgag ttgcctggtc
      841 ttctcgaaga caatcatgcg accattcggt gttagcgctt cattgagtcg gtcgaggcgt
      901 actccaatcg cagttcgctg ctcaaactcc ttttgttgac gggcatcgcg cgcacgctcg
      961 aaggtctgcc agccctggct gggaattccc ggatcctgct cggcctggac gagggcatgg
     1021 gtggcaataa tcagatcgta cgtccccgaa agagcgctgc actccacatc gacacattcg
     1081 aagcgaacat ttgtcagtcc acgcttctgg gcttgctgtt gtgcgaccga aatcgaggcg
     1141 ggcgagcgat cgatgccgag aaattgtttg tcgctgaatt gccttgcata gaacgttgtc
     1201 agaatcccga caccgcagcc gaagtcgagg atgctctcgg cccctctgat gtgtgcggct
     1261 atgcgtgaac caatttcact atagtactca tagcgctggc tatagaggac cggcaggata
     1321 tggggctgag ccgtcagatc atagaaggcg atctcgtcgc gacgatcgcc ttcccgtttt
     1381 cgttcaattt gtcggttaag gtgatgcagg tccgtggaag aaagcctttc acgttgccac
     1441 gcgaagtact ccgcatcagt cgtgaaatgc ttgagccccc accacgtaag gtgcgcttcc
     1501 agagcgtctt ctaaggatga cctcgtcaca tactctcagg gtcgcttcaa catgatggca
     1561 tcaaaataag tttccgggat cgggtgaggt agacgcaatt gccctgttcc aaacacaaca
     1621 tacttttcac aagtcaattg ctcgaggccg atcggcccct tcgcatggat acgtgacgtg
     1681 ctgagcccaa cgtctggacc ggctccataa ctgtccgccg cgttgagtcg cgaagaagca
     1741 tttaccagga cggcgctcgc atccacttcg cgcgcaaatc gcatggctga gtcatagcga
     1801 gcagtggcga tgactgccgt gagacagggc ccgtgctcgg caatgtgtgc caacgcctcc
     1861 tccatgttga cgaccatctt gaccgccaac actggcgcct ggaactgctt gtcccaatcc
     1921 tcctccgtcg ccggtgtaat ggaggtatgt ccgctgatcg gcatttgccc catgagggcc
     1981 acggtcttcg aacatccccg cacctgaact ttgaattcta cgagtaggcg attgatcagc
     2041 gccggcaaga attgccgagc aatcacctgt tgcacaagca gcgtatctag cgcattcgcc
     2101 gcaccggccg attgcacttt ggaattcacg actaggtttt ggaccatggg caactcggca
     2161 tcggcatcca catagagatg cgtgagccct ccatcgtggc agagcaccgg cattttggcc
     2221 tgatcgagca ccgctttgcg aagcccaggc ccgcctcgcg gaatgattgc gtcgaggttt
     2281 ttcccggcac gaatcagttc gaccgccacc tctttttccg gacgatccac aagaatccac
     2341 gcccccgccg gcacaccagc ctgttcggcc gcctctcgaa tcccggtacc aatgacctga
     2401 tgggtgagtc cccactccgg tgccccgcga aagacgcaga cgttgcccga cttgagacag
     2461 agggcaatgg tttccaccgt gacgagcggc atcatctcgg aaatcacccc gatgaccccg
     2521 atcggcacac ggacccgact gacctgtagt ccatccggct gctcccagcg tttcgtcacc
     2581 actcccaccg gatccggcag atcggcgatg gcacggagtc gctccgccat ctcctttata
     2641 tcgtccgccg cgatccgcac gcgggcgact gcggccttca tccgatcctt gctcgtctcc
     2701 gctgcataca acttgcccac tgcctcaaca tcctgctcat tcgcagcgag aattttctcc
     2761 ttgtcagtgt caattcgatc tgccatcgcc cgcaaagcct tgtctttgat tgaacccgac
     2821 agcaatgcca ttggcttcat gacagttcga ggctttttca atagcttatc catatacagc
     2881 ttaactggaa cttcaggcat cacgaacccc cgtacaacgt gaatggctcg acgtgaattg
     2941 ttcttgtaca gacctcttgt cccacgacga ctcggcgact ataccatgcg atttttctgc
     3001 ccgtaaagcg gcccgcgctt attctggagc gacttgttct ctcctacgct gctcttcatg
     3061 tctcgctaga ctccacgcaa gccatcccca cagaagtgag atcggcacga gtacccaggc
     3121 caactgagtg gtcgtggccc cgaaggcttt tacgccgttg acgatccagc ctgtggccgc
     3181 atcccctccg cgcgagatcg ccgtatcgat gaagttcttg gccttgtatt tttcctcgcg
     3241 gctgacgacc gtaaacaaca cttctcgcgc tggttttgaa atcgcgtatt cccccactcg
     3301 ccgtacgacc gtgaacagaa tgaacacgac cagcaccggc atgagcccga ctgtgctaaa
     3361 cccaatgaca ctggcgattg gaagaaacac gagcgcggaa ccaagcccga gtgcactaat
     3421 catgcggccg gtgacgaatg cctgggtgat gaaggcaatg acgttgaccg cgagatcgac
     3481 aatggcgaac aaccgagttc gtgattctgg tgacgggatc tgcattccga tgagcctcgc
     3541 ctgttccaga tacagaaacg tggccgtcgt cgtcaggcaa aacaaataga cacagattcc
     3601 catgagatag ggcgacgtca gcgccagccg aataccgccc cagatgcttc cgccgatcgg
     3661 ctcgccctgt tgcccgaccg gagcgatgag atggtgccgc gcccatcgat caagttgatg
     3721 gatgcagata caacaaccga cgagaaacac cgccgagatc agcatcagtg ttgcaaccgg
     3781 tacgacaaac gtcagccctg ccgtgaggag agggcccgtc atggccccag tgcttccacc
     3841 tgccgcaatg acggcgaaaa gccgcgcgcc ttgttccggt gtaaacaggt ccgccatgaa
     3901 gctccagaaa accgagacga caaagaggtt gaataccgag agccacacga aaaacacgcg
     3961 tgccgtccat tcaggcgtga tatggctcat catcacgacg aagaaaccga taaggttggc
     4021 cgcgaagaac agatacaccg tgagcaataa ccgatggcgt ggcacgcagg cagagagata
     4081 cccgaacagc ggggtgacga caagcagcgt gacgaacgta ccggtgaaca tccagggcag
     4141 gtgttgcact ccgccctcta tcgccatctc gtcgcgcacc ggccgcaaga tgtagtatcc
     4201 gcacaggagg aaaaagaaat agaggaaggc ccatgccagt gggacgactt cctccgactt
     4261 ggcattcgtc agcgactgga tggattcccg caaggtggtc atgcggcggc atcctgccga
     4321 ggtatgcttt cccgtgcaag cggaaaattt gctacaatgc cgcggcggag ggactcgtga
     4381 caccagatga tgcgcgcgcg caatacaatt atctgatgac actgtgtatc agacgggagg
     4441 aagcgttcgg acccatggcc ttggcgtttc tccgcgagcc tcgatttgag caactcggcc
     4501 tgacgctgga agaacaattc aatctctata tggcgacggc ggaggccttt gcccccgaac
     4561 caaagcgata tacggcaaaa ctggaatgcc tgcagaaagc ccaggccttg cttccacgta
     4621 ctcgtttttt tgacccggga ctcgcgcgcc aactgcaaca ggatattcag aaaacgacca
     4681 ccgacctaga catctatacc caggcgatga aggtggcccg gcctcacgat ctcaccgcac
     4741 agcgtctcat cgtggaaact gaagtgccgg agtattttct cgatctcgcg caaaaacggg
     4801 ctgccgccta ttaccaaact aagtatcacc ttccctccga atcgaagacc gcgcagcact
     4861 tcggtggtcc gcccaaaaag tttgaaccgg acaacccgac gatccaaaag gaatttcccg
     4921 gcgcctgcgc ccctttcatg gctgcgcgga ccaatgcctt tcatctcctg ctgccgttcg
     4981 acctgaagat cagccgcacg ccagaagatc ccct
//
LOCUS       DATDNW010000054         6721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1166922, whole genome shotgun sequence.
ACCESSION   DATDNW010000054 DATDNW010000000
VERSION     DATDNW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166922"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1615)
                     /locus_tag="VI359_01390"
     CDS             complement(185..1615)
                     /locus_tag="VI359_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6972933.1"
                     /translation="MRGVALAAISMLLISCAMGPDYSRPDIPTSDSFRMAEEGKDLPS
                     LANVPWWELYRDEELQKLIRIALGENKDLKRSVATVDEFAARLLVAKTDFAPQMNATV
                     NAPVFGNTRNFQFRGFPNAFNYYVQGNLAWELDIWGRIRRSNEAALGDLFAREENRRA
                     IILQLVGGVAQAYFDLRQFDMQLEIATRTLQAWEESVRISQARLRQGLINRLDVDQFE
                     AERQNAAARIAELKRQMVQKENELSVLLGRNPGQVSRGRALTEQVMPPVVPAGLPSEL
                     LQRRPDVVQAEQGLAAATARIGVAKADRFPKLSITGLLGVASPQLSHLVASDTYFGVV
                     GPSLTGPLFNAQVLGFQQKVAEAQARQALAQYEQTILVAFREVEDALVGVSTAREQAA
                     AQEKQVNALQSALHLANLRYKGGLANYLDVLIAQRSLFDAELSLAATRRLHLVSVVQL
                     YKALGGGWSPEEAREGAGSKTEKGNG"
     gene            complement(1617..4805)
                     /locus_tag="VI359_01395"
     CDS             complement(1617..4805)
                     /locus_tag="VI359_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY6972934.1"
                     /translation="MISHFFIDRPIFASVLSIVIVVVGLVALQALPIAQFPEITPPVV
                     QIEADYPGASAEVVADSVARTIEVQLPGIDNLLYYDSTSTNDGHMTIRLTFEIGTDVD
                     IAQVQTQNREKLAEPQIPDEVIRQGISVKKVSPDLLAVVALSSTDPRHDTVFLSNYAI
                     LRVIDNIKRLKGVGDAYVFGQQNYSMRLILDPIRMAQLSLTPTDIANVVREQNRDFPA
                     GTVGREPAPKGTELTLPVITLGRLTEVKDFEEMIVRALPNGSMVRLKDVAKVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALDAVKRVRKEMGEVAKSFPPGVSWDIPYDTTRF
                     IEVSIDEVVKTLGEAMVLVILVVYLFLQSWRTTLIPGVAVPVSLIGTFAGMQALGFSI
                     NTLTLFGMILAIGLVVDDAIIVVENVQRHMAEECCSSREAAKRAMAEVTGPVIAIVLV
                     LCAVFVPVAFLGGITGQLYKQFAITIAMSVTISGLVALTLSPALCALVLTPGSEVQRK
                     GLFAIFNKVFGWAQHRYTTIVAGVLKRSALVLAVFGVLVFVLIGLFRAIPSSFLPDED
                     QGYFITMFQLPDGASKQRTDAVLDKVERFFLSNHAIHSTDAMSGLNFVFNTRGPNTAT
                     MFVPLHLWDERNEPSQHVKALIGSAYGEFAKIPEALILAFNAPSIRGLGATGGFSVQL
                     QDPSGGDFKKFAAVAQEFVTKAKQNPAIGAIGTSFRVSSPRLYAHVNRERAKALGVPI
                     SEVFDTLQAFFGNLYVNDFLKFGRVYRVQTEAEAQYRSKPEDISKIYVRALNGQSPSM
                     IPLDTVVTTEYTSGPDPVTHFNGFNTALVLGAAAPGYSSGQVLAALDQTAREVLVPRG
                     YEIDWSGISYQERLVGRQSVYAFAFGLLMVFLVLAAQYESWTVPFAVILAVPFGVFGA
                     LSAVWLRSMTNDIYFQIGLVTLIGLSAKNAILIVEFANQRYEHGLSAVDAAIEAAKLR
                     FRPIIMTSMAFILGVVPLVFATGAGAASRQSIGTGVFGGMLAATFLAIFFVPLFFVLI
                     RKLSQRGAGPGSVDGTVNARAIPDQSQTEGGH"
     gene            complement(4802..5998)
                     /locus_tag="VI359_01400"
     CDS             complement(4802..5998)
                     /locus_tag="VI359_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6972935.1"
                     /translation="MLVIGQTFRVTVMFLTCLSVIGLVGCKREAASTPAPPLPEVEVV
                     TVTSQTVPDEPEFIGQTESSRPVEIRPQVTGLIKERFFVEGRAVKKGDRLFQIDPVPF
                     EAALLSARAKVAQNEARLVQAKQNLARVRPLLAEQAVSQKDVDDAVAEELAAKAALEG
                     AKGELVKAKFDFDNTLITAPIDGMIERTRVYEGRLVSAQTDLLTIVHQVDPMYVTVSA
                     PETFLLKRRRDIAANKIQHPGVYQLRGIITFLDGTTYSHEGILDLLDVGMRTETGSRP
                     ARVTFPNPDQILVPGQFVKVRFTGTLKTGVVLVPQRAVQQGPKGPVLYVVGAEDKIEV
                     RDVQATNWEGNQWLIEEGVRPGEQVVVAGLHKVMPGAPVKPVPVSDPAAASVSQPTES
                     RTEHAK"
     gene            complement(6135..6461)
                     /locus_tag="VI359_01405"
     CDS             complement(6135..6461)
                     /locus_tag="VI359_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972936.1"
                     /translation="MLKITQHRDEASESVSFMLEGRLAGPWVKELEACWHQTEALPKS
                     RAVVDLTGVTFVDADGRALLTKMWQQGVRLHAIGCLNNCIVEEITKAGCASSSRPRRK
                     GKMEGT"
BASE COUNT         1402 a         1994 c         1914 g         1411 t
ORIGIN      
        1 cgtaggctcg tcgttcaatg tcttcacggg taggttgcgg ggaaggcttg gctcgttttg
       61 ccgttgaggt ctggcgaccg gttttcgccg ctggtttctt ggattctcgc ttcggcatgg
      121 ctgcctcctt ggtgaaggtc aagaactgac catcggtggg aagtttctcc ggcttcgaat
      181 tatctcaccc attgcccttc tctgtcttgg aaccagcacc ctcgcgagcc tcttctggcg
      241 accagcctcc acccaaggcc ttgtagagtt gaaccaccga taccagatgg agacgacggg
      301 tggcagcgag cgacagttcc gcgtcgaaga gactccgctg tgcaatgaga acatccaaat
      361 agttcgcgag cccgcctttg tagcgtagat tggcgaggtg cagggcggac tgcagcgcat
      421 taacctgttt ctcctgcgcg gcagcctgct cacgggcggt gctgacacca accaacgcgt
      481 cttccacttc cctgaacgcg acgaggatcg tctgctcgta ttgggccaag gcctgcctgg
      541 cttgggcctc agctactttc tgttggaagc ccaaaacctg cgcattaaac aacgggcccg
      601 ttaagctggg gcctaccacc ccgaaatagg tgtcgctggc caccaggtgg gacaactggg
      661 gactggcgac acccaagagg ccggtgatgc tgagcttggg aaaccggtct gccttggcta
      721 caccgatcct ggcggtcgct gccgcgaggc cttgctccgc ctggaccaca tcggggcggc
      781 gctggagcag ttcggaggga aggcccgccg gcaccaccgg tggcatcacc tgttcggtga
      841 gcgcccgacc ccgggagact tggccggggt tcctccccaa cagcacgctg agctcgttct
      901 ctttctgaac catctgccgc ttgagttccg cgatgcgcgc cgcggcattc tgccgctcgg
      961 cctcaaactg atcgacatcg agccgattga tgagcccctg tctaagccgc gcctggctga
     1021 ttctcaccga ttcttcccat gcctgtagcg tgcgcgtggc gatctccaat tgcatgtcga
     1081 actgtcgaag atcgaaatag gcctgggcca ccccgcccac gagttgcagg atgatggctc
     1141 gtcggttctc ttcccgagcg aataagtcgc caagagctgc ctcattggag cggcggatcc
     1201 gtccccagat atcaagctcc caggcgagat tcccctgcac ataataattg aatgcgttgg
     1261 ggaatccgcg gaactggaaa ttcctcgtat tgccgaagac cggcgcattg accgttgcgt
     1321 tcatctgagg agcaaaatcg gttttggcga cgagcagccg agcggcgaac tcatcaactg
     1381 ttgcgaccga tcgtttgaga tccttgttct ccccgagcgc gatacggatc agcttctgga
     1441 gctcctcgtc ccgataaagt tcccaccacg gcacattggc gagggagggt aagtctttcc
     1501 cttcttcggc catgcgaaag gagtctgacg tcggaatatc aggacgagaa tagtccggcc
     1561 ccatggcaca ggagatcagc agcattgaga tcgctgccag ggctacgcca cgcatatcaa
     1621 tgccctcctt ccgtttgtga ctgatctggt atcgcacgcg cgtttacagt tccgtccacc
     1681 gaacctggac cagcaccgcg ctggctcagt tttcggatca gcacgaaaaa gagcggaacg
     1741 aagaaaatag ccaggaatgt cgccgccagc attccgccga acacgcccgt cccgatagac
     1801 tgccggctgg ctgctccggc tccggtcgcg aacaccagcg gcaccacgcc gagaatgaag
     1861 gccatcgacg tcatgatgat tgggcggaac cgaagtttgg cggcttcaat cgctgcgtct
     1921 accgcagaca atccgtgctc gtagcgctga ttggcgaatt cgacgatcaa gatcgcgttc
     1981 ttggccgaga ggccgatcag cgtgactagc ccgatctgaa aatagatgtc gttggtcatg
     2041 ctccgaagcc acacggcgga cagggctcca aagacgccga agggcacggc aagaatcacg
     2101 gcgaaaggaa cggtccagct ctcgtactgg gcggccagca caagaaatac catcagaagg
     2161 ccgaacgcga acgcatacac cgattgtcgc ccgaccaagc gttcttggta tgagatcccg
     2221 ctccagtcga tctcatagcc tcgagggacg agcacctccc gggccgtctg atccagcgct
     2281 gcgaggactt gtccggaact ataccctgga gccgccgctc ccaagacgag agccgtgttg
     2341 aatccattga agtgtgtcac cgggtccggc ccgctggtgt actcggtcgt gacgactgtg
     2401 tcgagtggaa tcatgctcgg gctctgacca tttaacgctc gcacgtaaat cttggaaata
     2461 tcctcgggct tggatcgata ctgggcctct gcttccgtct gcacccgata gacacggccg
     2521 aacttgagga aatcattgac gtacagattg ccgaaaaagg cctggagcgt gtcgaagacc
     2581 tcggagatcg gcacgccgag cgccttggcc cgctcacgat tcacgtgggc atagagccgg
     2641 ggagagctca cgcggaagct ggtcccgatg gccccgatgg ccggattctg cttggccttg
     2701 gtgacgaact cctgagccac agctgcgaac ttcttgaagt ctcctccgct ggggtcttgc
     2761 agttgcacag aaaacccccc cgtggcacca agccccctga tggatggagc gttgaaggcc
     2821 aggataagcg cctcagggat cttcgcaaat tctccgtagg ccgaaccgat gagggctttg
     2881 acatgctggc tcggttcatt ccgttcatcc cagaggtgca acggcacaaa catcgtggcc
     2941 gtattgggcc ctcgcgtgtt gaagacaaag ttgaggccgg acatggcatc tgtggaatgg
     3001 attgcatgat tcgagagaaa gaaccgttcg actttgtcga gcaccgcatc agttcgttgc
     3061 ttggacgctc cgtcaggaag ctggaacatg gtgatgaaat aaccctgatc ttcatccggc
     3121 aagaagcttg acgggatcgc cctgaataga ccgatcaata caaacacaag aaccccgaat
     3181 accgccaaga ccagcgctga ccgtttgagg acaccagcca cgattgtcgt atagcgatgc
     3241 tgcgcccacc cgaagacttt gttgaagatc gcaaaaaggc ctttccgttg aacctcgctc
     3301 cccggcgtta agaccagggc acagagggcg gggctgagcg tgagggcgac cagtcctgag
     3361 atcgtgacgg acatggcgat ggtgatcgca aattgcttgt agagctgacc ggtgataccg
     3421 cctaagaagg ccaccggcac aaacacggca cagagcacga gcacgatggc gatcacgggc
     3481 ccggtcactt ccgccatggc ccttttggcg gcttcccttg atgagcaaca ttcttccgcc
     3541 atgtgacgct ggacgttctc caccacgatg atggcgtcat cgacaaccag cccgatcgcg
     3601 agaatcatgc cgaagagggt cagggtgttg atggaaaagc cgagggcttg catgccggca
     3661 aaggtgccga tcagggagac cggcactgcc acaccaggaa tcagcgtggt acgccagctt
     3721 tgaagaaaga ggtacacgac caagatgacc aggaccatcg cttcacccaa ggtcttgacg
     3781 acctcgtcaa tcgacacctc gatgaatctg gtcgtgtcat acgggatgtc ccacgacact
     3841 ccgggtggaa aactcttggc cacttccccc atctccttgc gaactcgttt aacggcatcg
     3901 agggcgttgg cgcccggaga caggaacgtc aagagaaaca cgttgggctt gccgttccac
     3961 cgtccttcga gagtatagga ctgggcgcct agctcgacct tggcgacatc cttcaggcgg
     4021 accatggagc cattggggag ggctctgacg atcatctctt cgaagtcctt cacctccgtc
     4081 aggcggccta gcgtaatcac gggaagcgtc aattctgtgc ctttgggcgc cggctctcgc
     4141 ccgacggtcc cggcgggaaa gtctcgattc tgctcccgga cgacgttcgc gatatcagtc
     4201 ggcgtgaggc tcaattgcgc catccgaatc ggatccagga tcaaccgcat cgagtaattc
     4261 tgctgcccga atacataggc gtctcccact cctttgagcc gtttgatgtt gtcgatgacc
     4321 cgaaggatgg cgtaattgga cagaaagact gtgtcgtgcc tgggatcggt ggagctgagc
     4381 gcgacaacag cgagaagatc gggcgagact tttttcacgc tgatgccttg gcgaatgacc
     4441 tcgtccggga tctgcggttc cgccagcttt tcgcggtttt gggtctggac ctgtgcgatg
     4501 tccacgtccg ttccgatctc gaacgtgagc cggatggtca tgtggccgtc attggtgctg
     4561 gtggagtcgt agtacagaag attgtcaatg cctgggagct gcacctcgat ggtgcgcgcg
     4621 actgaatccg cgaccacttc cgcgctcgcg ccgggataat cggcctcgat ctgcaccacc
     4681 ggcggtgtaa tttccggaaa ctgggcgata ggaagggcct gcaaggccac aagacccact
     4741 accacaatga cgatcgagag caccgacgcg aaaattggtc ggtcaataaa gaagtgagag
     4801 atcacttcgc atgctccgtc ctgctctccg tcggttggct cacggaggcc gcagccggat
     4861 cactaacggg gaccggtttc accggggctc ctggcatcac tttgtgaagt ccggccacca
     4921 ccacctgttc accggggcgt accccctctt cgatcagcca ttggttgcct tcccagttgg
     4981 tcgcttgaac gtcacggacc tcgatcttgt cctcggcacc aacgacatag agaacaggtc
     5041 ctttcggacc ctgttgcacc gctcgttggg gaaccaggac cactccggtc tttaacgtgc
     5101 cggtgaatcg aaccttgacg aactgtccgg gaaccaggat ttgatctgga ttgggaaacg
     5161 taacccgggc cggcctggat cctgtttcgg tacgcatgcc cacatcgagt aaatccagga
     5221 tcccttcatg agaatatgtc gtgccgtcca aaaaggtaat gatgcctcgc agctggtaga
     5281 cgcccggatg ttgaatcttg tttgcggcga tgtctcttcg ccgtttcagc aggaacgttt
     5341 cgggggcact cacggtcaca tacatggggt ccacctgatg cacgatcgtg agcagatcgg
     5401 tctgggccga gaccaaccga ccttcataga ccctggtccg ctcgatcatg ccgtcgatgg
     5461 gagccgtaat gagcgtgtta tcgaaatcaa acttcgcctt taccaactcg ccctttgctc
     5521 cctccagcgc cgccttcgcc gccaactcct ccgcgacggc atcgtcaaca tccttctggc
     5581 tcaccgcctg ctcagccaga agcggtctga cgcgggcgag attttgtttg gcctgcacca
     5641 gccgtgcttc attctgggcc acttttgctc ttgcactgag caaggctgct tcgaacggca
     5701 ccggatcgat ttggaacaga cgatcgcctt tcttgacagc acgaccttca acgaagaagc
     5761 gctctttgat gagcccggtc acctgtggcc ggatctcaac cgggcgcgac gattcggtct
     5821 ggccgataaa ttccggctca tccggcaccg tttgggaagt gacggtcacg acctcgacct
     5881 cggggagggg aggggcggga gtggaagccg cttctcgttt gcagcccacc aagccgatca
     5941 ccgataaaca tgtgaggaac atgacagtca ctcgaaatgt ttggccgatt acaagcatgg
     6001 tccattctcc gtgcgaatga tcttgcgatc gattgttctc ttttcgcccc tgcggaatgg
     6061 tcattctagc ggtatgttct tatgctcaac aaggtccatc atgagcaacg ccaggaacca
     6121 agacgggcgc gggctcaggt tccttccatt ttgccctttc gacgtgggcg ggacgagctg
     6181 gcgcaaccgg ctttcgtaat ctcttccaca atgcagttgt tcagacagcc gattgcatga
     6241 agtcgcacac cctgctgcca cattttcgtc aggagcgccc tgccgtcggc atccacgaat
     6301 gtgacgccag tcaagtcgac taccgctcga ctctttggaa gcgcttccgt ctgatgccag
     6361 catgcctcga gttccttgac ccaaggcccc gcaagtcgtc cttcgagcat gaacgagact
     6421 gactccgacg cttcgtcccg gtgttgggtg atcttcaaca tctttcgttc cccctgcaca
     6481 cactgagggc aaggggcgtt ccagcaacac caatcggcca tatccatcta acctattgaa
     6541 agaactatgc gaattgtatg tgcttgcgcc cgaatagcct ggctgacgat agcgttctgc
     6601 cgatatcccg gcacacgccg aaccatcggc aggaaactgg aagactgtga ctcaatagcg
     6661 tcggcgcgtc gagaaacgcc gagatcattt ctcggcgatg gtcttcagat tggcaagacc
     6721 t
//
LOCUS       DATDNW010000055        13585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1303006, whole genome shotgun sequence.
ACCESSION   DATDNW010000055 DATDNW010000000
VERSION     DATDNW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303006"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VI359_01410"
     CDS             <1..631
                     /locus_tag="VI359_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972937.1"
                     /translation="TTSFAGAAFDHTGIVTGCATCHNGTTALGKPATHIPTTQGCETC
                     HKSTTAFGPGTPMNHTGITTGCATCHSGGYAGVVSKPANHFPTTAPCETCHKSTTSFA
                     GTTYNHAGIVSGCATCHSGGYAGVVSKPANHFPTGTACETCHKSTTTFSGTRMVHDGG
                     MTGKCATCHERGMNWVGGIVTRPAGHTGSRGAPNSCDNAGCHNTTTFSK"
     gene            836..3133
                     /locus_tag="VI359_01415"
     CDS             836..3133
                     /locus_tag="VI359_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972938.1"
                     /translation="MRARYHTMLRHVAAFVFLSGLFSLAGLLFPVWTTAQVLDRIEIV
                     ETSGVAEIHIVFNTRALYLRHTPSDKGDLIRIFLGFPDLDRSQRFARELAASPPSDLV
                     PKFSVIFPDQTTNELSIRFEKPVRFRISQRDIRSASRIVIAVKLDRPVELPPVPPEVK
                     IPQTTPPEAKGPKQSFDIPSFHPGMNVETYAEDLMKLGHKALIAGENEKAVQIFNALL
                     NLPPNKQSQGAQEWIGVARQRSGEYVKAKAEYELYLKLYPEGDGAVRIQQRLAALQEA
                     MSQLAQTKKGRPTRKIDEKQLYGSVYEYYYGGYSQTKITDKTTNTTTKINTQDQSLLQ
                     SAFDVTGRYRKDEYDNKLVVRGTQTYDALANTDARRNVSRLRALYYEHSSQDSYLVRI
                     GRQPGNVGGVLDYRFDGAWFRYTAVPQFLNVNLLAGQPRQFSTSPTYVPDDPRNFRAS
                     LNPYFYGANVDIGPLGEAWSGNAYFFNQMINGVVDRRAVGTELRYAANGKNAFGLVDY
                     DISYGVLNIAMLNGTWVTEGTTYTFLADHRRVPYLQTSNALFGTPGASVDMINTTNES
                     LLRTQAKAITATSDLFLAGVLHAVSKDWQLGGDVRLNRISGTSASNCLIILPGTSTLF
                     LNPNSITDAPCSLQALPGTGNIWTYTVQAIGSNFPIDNMTFVTNASYITSSAYRGESL
                     TLNSLARIGQQWQFDTFLILYHQTDSNNVELYRATPTVRMDYRFWNSWTLEASGGVER
                     TLTDSASQKDSTWRQFFFGGLRWDF"
     gene            3140..3760
                     /gene="ccmA"
                     /locus_tag="VI359_01420"
     CDS             3140..3760
                     /gene="ccmA"
                     /locus_tag="VI359_01420"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY6972939.1"
                     /translation="MLDAIDLTCVKGERNLFQDLTFRVPQGGFLSVMGNNGSGKSTLL
                     RLLTGLLPPDSGRVCWDGRDITELKDQYVSRLSYVGHRNALKDDLTPIENIRATSSML
                     GQTLTFKAAQTTLDIVGLSRTFHLFPTKLLSEGQRRRVALARLWSCSRPLWVLDEPFT
                     ALDGHGSEVLRERLTAHVRDGGIVVMATHQEVNLEAGSASHLRLGG"
     gene            3771..4439
                     /gene="ccmB"
                     /locus_tag="VI359_01425"
     CDS             3771..4439
                     /gene="ccmB"
                     /locus_tag="VI359_01425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY6972940.1"
                     /translation="MTQAFFGVVWRDLLLAIRRRSDVVTSLMFFIIVTSLFPLGIGPD
                     QAVLRTMAPGVIWVAALLSSMLSLQRLFASDYADGVLDQMVLSPHPLAVLAGGKIVAH
                     WIVAGLPIVLLSPVMALQFDLSGSSIIVLVVTLLLGTPTLSLLGSIGSALTLGVRGGG
                     PLMALLVVPLFIPILIFGAGAVVANESGLGVEANVSLLGAGLLLGAALAPIATAAALR
                     IAVA"
     gene            4436..5185
                     /locus_tag="VI359_01430"
     CDS             4436..5185
                     /locus_tag="VI359_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY6972941.1"
                     /translation="MIGQHLNTPGINWFRYSSLQAIYPLAGRLIPWFAGLALICSLIG
                     LYIGLGLAPTDYQQGQAYRIIFVHVPAAWMSMFLYVVMACWAAIGLGLKAKPSFLMAQ
                     AIAPTGALFTFLALVTGSMWGKPTWGTWWVWDARLTSELILFFLYLGTMLLHASIDDP
                     RRADRAAAVFAIVGVVNVPIIYFSVQWWNTLHQGASVSMTAAPKMAAMMVTAMLIMMV
                     AFWLYSIAVVLARMRCEILEREGRSAWLRLQ"
     gene            5195..5374
                     /gene="ccmD"
                     /locus_tag="VI359_01435"
     CDS             5195..5374
                     /gene="ccmD"
                     /locus_tag="VI359_01435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY6972942.1"
                     /translation="MQWNSLDDFLAMGGYGLYVWASFGTTALCLMVEVAAVRRRYRLG
                     CQAVRAGQQSDTEPG"
     gene            5371..5838
                     /gene="ccmE"
                     /locus_tag="VI359_01440"
     CDS             5371..5838
                     /gene="ccmE"
                     /locus_tag="VI359_01440"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY6972943.1"
                     /translation="MTTRQKRFALIGLGLVAMALATALVLNAFRSNLVFFFTPTQVAN
                     GEVPKGRSFRIGGMVETGSLARERDGLTVHFVVTDTVKRVPVTFKGILPDLFKEGKGA
                     VAQGQLDADGTFRASEVLAKHDENYMPPEAAAALEQAKAAGAQGSRTLVTGPQ"
     gene            5847..7838
                     /locus_tag="VI359_01445"
     CDS             5847..7838
                     /locus_tag="VI359_01445"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY6972944.1"
                     /translation="MIPEIGHFALMLALCVAVVQAVLPVWGAATNNAAFMAIAKPAAH
                     GQFLLVALAFGCLAYAFAVKDFSVLYVAANSNSQLPLHYRLAAVWGAHEGSLLLWTFI
                     LTVWMVAVTLFSRQLPLPMRARILGVMGFVSVGFLLFMLTVSSPFERLIPAAADGRDL
                     NPLLQDPGMVMHPPMLYMGYVGFSVAFAFAIAALLGGNLDAAWARWSRPWTTVAWCFL
                     TIGIALGSGWAYYELGWGGWWFWDPVENASFMPWLAGTALIHSLAVTDKRGSFKVWTV
                     LLAILAFSLSLVGTFLVRSGVLTSVHAFATDPKRGLFILAYLAIVIGGSLTLYAWRAP
                     RVGLGGRFAFVSRESMLLVNNVLLVVATGSVLLGTLYPLFLDALGMGKLSVGPPYFDT
                     VFVPLITPALFLMGIGPLAQWKQASLPDLAIRVRWAFAVSVATALLLPLVVGKWTPLV
                     SLGLLLAMWILATAVLNVRERLTHVTVNSLWSGLASFPRSYWGMLLAHCGIAVFIIGV
                     TFVKGFETEKDLRMNVGETATIGGYQFRFDGVQEIKGPNYRAGRGIFFVTKGGRPITE
                     MYPEKRFYPVQNQAMTEAAIDTGFLRDLYVSLGEPLDGGAWSVRLYHKPFIDWIWGGC
                     LLMAFGGVLAISDRRYRLSWRKQDETAPVLATPRPSEVA"
     gene            7835..8362
                     /locus_tag="VI359_01450"
     CDS             7835..8362
                     /locus_tag="VI359_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY6972945.1"
                     /translation="MKRFLIPLGSFAVIMGFLAAGLTLNPREVPSPFIGKPAPDFTLP
                     QLHDPEKVFSPKDLAGQVWLLNVWASWCSGCKDEHPVLMQLAQSGSVPIYGMDYKDQR
                     AEALSWLKRQGNPYPLSAMDEAGRIGIDYGVYGVPETFVIDKKGMIRYKQIGPLDQEA
                     LDRKVLPLVRELQNQ"
     gene            8371..8823
                     /locus_tag="VI359_01455"
     CDS             8371..8823
                     /locus_tag="VI359_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY6972946.1"
                     /translation="MFLLCLLPGVVFAQEARPLGENPAVEARLKQLAVELRCLVCQNQ
                     TLADSNAPLAEDLRREVREMIARNMTDDQIVDFLVTRYGDFVRYRPPFKVTTLLLWVG
                     PFLLLAAGATVLMLSLRRRAQKTEEGMLSQEEQARAARLLEGKGGSRT"
     gene            8820..10106
                     /gene="ccmI"
                     /locus_tag="VI359_01460"
     CDS             8820..10106
                     /gene="ccmI"
                     /locus_tag="VI359_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY6972947.1"
                     /translation="MSILFVLIAATLTIGVMLLLVLPLLRPPSQADARETDKRLSVYR
                     QQLAELEQDRAACTLTQEQFEHSRRELERRVLEETTSDSVPPGPSRWQLSSRTVALAL
                     IVTIPIAVFLLYWTLGNPLALIHPGAQAKETGGNSEYAHQSSTSLDTLTDRLKQRMEQ
                     SPNDGAGWALLARSYVELGRHGDAVTAYEKAVALIPEDAQLLVDYADALAMARGRKLE
                     GKPEDLIARALKLDPRNVKGLMLAGTIAYDRKDFPTALKHWEQAQKGLPPETEPELVQ
                     ELAANINEVRGLLGLAVGGGKMASMPTDVPTSTKQVRQNLAISGMVTLAPSLAGKGSP
                     VDTLFVFARETNGPPMPVSIVRATKQDLPFTFRLDDSTSPMPSRKLSDVGTVVIVARL
                     SKSGEAMPKSGDVQGMSQPVKPGAKGINVILDREIP"
     gene            10122..11435
                     /locus_tag="VI359_01465"
     CDS             10122..11435
                     /locus_tag="VI359_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY6972948.1"
                     /translation="MSMSAIITLLYVLPLIIALILYYRRHRKRETHYRTRLAEMVKSG
                     ITEPLTLHPVIDTNKCIGSGACVRACPEHSLGIIRGKAVLVEPDHCIGHGACETACPV
                     EAITLVFGTEKRGIDIPQVKPTFETNVPGIFIAGELGGMGLIRKGVDQGKRAIESIRK
                     NRSKGTELDVVIVGAGPAGLSASLAAKEAGLRFVTIEQEDGVGGSVYHYPRRKLTMTA
                     PMKLPIVGKLNKHQISKEELLEFWNRIIRQTGLTINFTERMESITKAGRGFVVKTSKA
                     TYKATNVLLAIGRRGTPRKLGVPGEEQSKVVYSLIDSEQYRGQHVLVVGGGDSAAEAA
                     LAIAAERGTVVTVSCRGDEIFTRPKERNRRQLKMLSDQGKVKVFLHANVKQIGINHVT
                     LDREGTVIELKNDAVIVCAGGTLPTPMLKEIGVMVSTHYGTPVAR"
     gene            11509..12033
                     /locus_tag="VI359_01470"
     CDS             11509..12033
                     /locus_tag="VI359_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972949.1"
                     /translation="MLSFSQRIYKRPSGRNFARYVHLLSASALLVSCVSIRVEYLADE
                     TYPSREHAASVEWLSAEPSEPYIKIARIVADSTSGSQETLRQAILDRARRLGADAVID
                     EKAATVSSRAPSPNYERGVLGPAGASFDLYGYGHSTPYAPAPYLLSQGATDQPRLDEY
                     LSGMAIRFSGSPRR"
     gene            complement(12294..13316)
                     /locus_tag="VI359_01475"
     CDS             complement(12294..13316)
                     /locus_tag="VI359_01475"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY6972950.1"
                     /translation="MRQTAKQKFLVFLALPLSLVSCGPGPQGPGPQVGGGIGGTGSVK
                     TVSSGPVTKFGSVFVSGTEYDNSNTIYCIDDEPCGPQNTLKIGMVVLVDGRISKEYSS
                     GQPLARIADTITYEETVEGIVQSVASDGLSLFVLGQLVRLNQKTVIDPSITGQSVHSL
                     IPGLDLVEVSGFVTGDGEILATLIMKQADPPHYEIQGSVKNHDVKQKTFEIGALVVNY
                     SSADTSEMPSPGSATWNNIVVQVRGDQSSQGGPGPHGAKLTATLVKPLTLGVEDDSEA
                     EVEGVILQVNTPGDFFVNNTHVMTTTSTIIEGGTINDLVMGAQVEIHGSLGNGVLQAS
                     HISLDD"
     gene            complement(13331..>13585)
                     /locus_tag="VI359_01480"
     CDS             complement(13331..>13585)
                     /locus_tag="VI359_01480"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY6972951.1"
                     /translation="TIDHNVYQKPSKPRFQRKVMYDNVPVEAAKEFRILAAAKGQELL
                     ETIDRWLAHRDRDVNPGSKGSGRVRVGLGIYHFEERLDSD"
BASE COUNT         2816 a         3709 c         4036 g         3024 t
ORIGIN      
        1 cacgacatcg tttgcgggag ccgcgttcga tcacaccggc atcgtgaccg gctgtgcgac
       61 gtgccataac gggacaacag cgttaggcaa gccggccacc cacattccca ccacgcaagg
      121 gtgcgagacc tgccacaaat ccacgacggc cttcggcccc gggacgccga tgaaccacac
      181 cggcatcaca accgggtgcg cgacctgtca tagcggcggg tatgccggcg tcgtgagcaa
      241 gccggccaat cacttcccga ccaccgcgcc ttgcgagacg tgtcataagt cgacaacctc
      301 gttcgcaggg accacctata accacgcggg gatagtgtcc ggctgcgcga cctgccatag
      361 cggcgggtat gccggcgtcg tgagcaagcc ggccaatcac ttccccaccg gcacagcatg
      421 cgaaacgtgc cataagtcca cgacgacttt cagtgggacg agaatggttc acgacggggg
      481 gatgacaggg aaatgcgcga cctgccatga gcgaggcatg aactgggtag gaggaatcgt
      541 gaccaggcca gcgggccata caggaagcag aggggcgccg aactcttgcg acaatgccgg
      601 ctgtcacaat accacgacct ttagcaagta aggagtgatg ttgcctgatt gatcaagcgg
      661 gatacccggc aggatacgtc agggccgggc gttcaggtgt gatgtgtgtg aacgatgtgt
      721 ggatagtcgg tgatggctaa ccgtccgaac agcacacgat gccgctacgg tctgcacctg
      781 aatttcacta cattaaaatc cgtaggattt ctgccgaaca ctcagaggtt atgtattgag
      841 agcccgttat cacacgatgt tgcgccatgt cgcggcgttt gtctttttgt cgggattgtt
      901 ttcgcttgct ggtctgctgt ttcctgtatg gacaacggcc caagtgctcg accgtatcga
      961 aattgttgaa acctctggtg tggccgaaat tcatatcgtg ttcaataccc gagcactcta
     1021 cttgcgacat acgccgtctg ataagggcga tctcatccgc atcttcctcg gctttccaga
     1081 cctcgatcgt tctcaacggt ttgcccggga actcgccgca tcacccccca gtgaccttgt
     1141 gccgaagttc tccgttatct ttccggatca gacgacgaac gagctctcca ttcgattcga
     1201 aaaaccggta cggttcagaa tcagtcagcg ggatattcga agcgctagtc gcattgtgat
     1261 tgcagtcaaa ttggatcgac ctgtagagct ccctccggtc ccgccagaag taaagatccc
     1321 ccagactact cccccggaag ccaagggtcc gaagcagtct tttgacatcc cgtcattcca
     1381 tcccggaatg aatgtggaga cgtatgctga ggatttgatg aagctcggcc ataaggcgct
     1441 gatcgctgga gagaatgaga aggcagtgca gatcttcaac gcactcctta atctcccgcc
     1501 aaacaagcag tcacagggag ctcaggagtg gattggagtg gccagacaac gaagcgggga
     1561 atatgtgaaa gcgaaggcgg aatatgaact ctatctcaaa ctctaccctg agggagacgg
     1621 agcggtaagg atccagcagc ggctggcggc attgcaggag gctatgagtc agcttgcaca
     1681 aaccaaaaaa ggcagaccaa ctcggaagat cgacgagaag cagctctatg gcagtgtgta
     1741 tgagtattac tatggtggat attcacagac gaaaatcacc gataaaacga ccaataccac
     1801 gacgaaaatc aatacccagg atcagtctct gcttcaatct gccttcgatg tcaccgggcg
     1861 ttaccggaaa gatgagtatg acaacaagct cgtggtacgc gggacgcaga cgtacgacgc
     1921 attagctaac accgatgcaa ggcgcaatgt cagccgtctg agagcccttt actacgagca
     1981 ttcgagtcag gattcatatc tcgttcgcat cggtcggcaa ccaggcaatg tcggaggcgt
     2041 cctggattat cgcttcgatg gtgcctggtt ccgctacacg gcggtgcccc aattcctgaa
     2101 tgtgaacctc ctcgcaggcc agccgaggca attcagcacg agtcccactt atgtcccgga
     2161 cgatccgcga aacttccgag ccagcctgaa cccatatttc tacggtgcga atgtggacat
     2221 tggtcctctc ggggaggcct ggagtggcaa tgcctacttc ttcaatcaaa tgatcaacgg
     2281 agtcgtggat cgtcgagccg tcggcacaga gcttcgctac gccgctaacg gcaagaatgc
     2341 gttcggtttg gtcgattatg atatttcata tggtgtgctc aatatcgcga tgctcaatgg
     2401 gacatgggtg acagagggga ccacctatac gtttttagct gaccaccgaa gggttcctta
     2461 cctccaaacc tcgaacgcgc tcttcggcac cccgggagcc tcggtcgaca tgatcaatac
     2521 gacaaacgaa agtctgttgc gcacccaagc aaaagccatt acggctacca gcgatctctt
     2581 cctcgcgggc gtgcttcatg cagtcagcaa ggactggcaa ctcggtggcg acgtgcgtct
     2641 taaccgtatc tccggcacgt cagcgtcgaa ctgtttgatc attttgcccg ggaccagtac
     2701 gttgttcctc aatcctaatt ccatcactga tgctccatgt tccttgcaag cactccccgg
     2761 tactggcaat atctggacgt atacagttca agcgatcgga tcgaacttcc cgatcgacaa
     2821 tatgacgttc gtgacgaacg cgagttacat cacaagctca gcctaccgag gagaatcgtt
     2881 gactctcaat tctctcgctc gcatcgggca gcaatggcag ttcgacacgt tcctcattct
     2941 ctatcatcag acggatagca acaacgtaga gctgtatcgc gccacaccca ccgtgcgaat
     3001 ggactatcgc ttttggaata gttggacgct cgaagcctcc ggaggcgtcg aaaggaccct
     3061 aaccgatagc gcttcacaaa aagactccac gtggcgacag ttttttttcg gtggtctgcg
     3121 ctgggatttt taataggata tgttggacgc catcgacttg acgtgcgtga aaggtgagcg
     3181 gaatcttttt caggacctga cctttcgggt tccgcaaggc ggcttccttt ctgtcatggg
     3241 gaataacggt tccgggaagt caacgctcct ccgtctgctc accggcttgt tgccccctga
     3301 cagcggccgc gtttgttggg atggtcggga catcacggaa ttaaaggatc agtatgtctc
     3361 acggctgtcc tatgtcgggc atcggaacgc gctcaaagac gacttgacgc cgatcgaaaa
     3421 cattcgggcc acgtccagca tgctcggaca gacgctcacg ttcaaagctg cccagaccac
     3481 gctcgatatc gtgggtctga gcagaacttt tcacctcttc ccgacgaagc tcctgtcaga
     3541 aggacaacgg cgacgtgtcg cgctggcgcg gctgtggtct tgttcccggc ctctctgggt
     3601 gttggatgaa ccgttcaccg cgctggatgg tcacggctcg gaagtgttgc gggaacgact
     3661 gactgcacat gtgcgtgatg gagggatcgt cgtcatggcg acgcatcagg aagtgaactt
     3721 ggaagctgga tcggccagcc atctgagact gggggggtaa aggagcccgg atgacccagg
     3781 ccttcttcgg ggtggtatgg cgcgatctcc tgcttgccat cagacgccgc tcggacgttg
     3841 tgacgtcact gatgtttttt atcattgtca cgagtctgtt tccgttggga atcggaccgg
     3901 atcaggcggt ccttcggacg atggcgccgg gagtcatttg ggtggctgcc ctcttgtcgt
     3961 cgatgctctc gctgcaacgc ttgttcgcat cggactatgc cgacggggtg ctggatcaga
     4021 tggtactgtc gccgcatccc ttggcggtct tggccggcgg caagattgtg gcccactgga
     4081 tcgtcgccgg acttccgatc gtgctgctat ctccggtgat ggcgttgcaa ttcgacctca
     4141 gcgggtcgtc gattatcgtg ttggtcgtca cgctcttgct gggaacgcca acgctgagtt
     4201 tgctgggttc aattggatcg gcgctcacac ttggggttcg aggcggaggt ccgttgatgg
     4261 ccttgctggt ggttccattg ttcatcccga ttttaatttt cggcgctggt gctgtggtcg
     4321 ccaatgagtc tggattggga gtcgaggcaa atgtttcgtt gctcggagcc gggttgttgc
     4381 tgggcgcagc actggcgcca atagccacgg ctgcggcgct gagaatcgcc gtcgcatgat
     4441 cggtcaacac ttgaacacac ccggtatcaa ctggtttcgc tattcctctc tgcaggcgat
     4501 ctatccgctg gcgggtcggc tcatcccctg gtttgccggc ttagcgctga tctgtagtct
     4561 cattgggctt tacatcgggt tagggctggc tccgacggac tatcagcaag ggcaggccta
     4621 tcgaattatc ttcgtgcatg tcccggctgc ctggatgtcg atgttcctct atgtcgtcat
     4681 ggcctgttgg gctgcgatcg gattgggact gaaggccaag ccgtcgtttc tcatggctca
     4741 ggccatcgcg ccgaccggcg cactcttcac cttcctcgcc ctcgtaactg gttccatgtg
     4801 gggcaaaccg acgtggggaa cctggtgggt gtgggatgcg cgacttacgt cggaactgat
     4861 cctcttcttc ttgtaccttg gaaccatgtt gctgcatgcc tcgatcgatg acccccgccg
     4921 ggcggatcga gccgcggcgg tctttgcgat cgtgggcgtc gtgaacgtac ccatcatcta
     4981 tttttctgtg cagtggtgga acaccttaca ccagggcgcg tcggtgagca tgacggcggc
     5041 gccgaaaatg gcggcgatga tggtgacggc gatgctgatc atgatggtgg ccttttggct
     5101 ctatagcatc gctgttgtgt tggctcggat gcgctgcgaa attctcgaac gagaaggtcg
     5161 atcggcttgg cttcgtttac agtaggttgt ggcgatgcaa tggaatagtc tcgatgattt
     5221 tttggctatg ggtgggtacg ggctctatgt gtgggcctca ttcggcacaa cggcactgtg
     5281 cctgatggtg gaagtcgcgg ccgtccgacg gcgctatcga cttggttgtc aagcggtccg
     5341 cgcaggacaa cagtcggaca cggagccggg atgacgacgc gtcaaaaacg attcgccttg
     5401 atcggtctcg gccttgtggc gatggcgtta gcgacggcgc tggtactgaa cgccttccgg
     5461 agcaatctcg tgttcttttt tacgccgacc caggtcgcca acggagaggt gccgaagggc
     5521 cggagctttc gaatcggggg catggtggaa acagggagtc tggcacgaga gcgcgatgga
     5581 ttgaccgtcc atttcgtcgt gacggacacc gtcaagcgcg tacctgtcac gttcaaggga
     5641 attctcccgg atctcttcaa ggaagggaag ggcgcggttg cgcaaggtca actggatgcc
     5701 gacggcacgt tccgcgcttc tgaagtctta gcgaagcacg acgagaacta catgccgcca
     5761 gaggcggccg ccgcgctgga acaggccaaa gctgccggcg cccagggcag ccggacattg
     5821 gtcactggtc cacaataaag gagcccatga ttcctgaaat cggtcatttt gcattgatgc
     5881 tcgcgctctg cgtcgccgtg gtgcaggcgg tcttgccggt ttggggtgcg gccacgaaca
     5941 acgcagcctt catggctatc gccaaaccgg cggcacacgg ccaattcctc ttggttgccc
     6001 tggcgttcgg ctgtctcgcc tatgcctttg cggtgaaaga tttctcagtg ctctatgtcg
     6061 cggccaactc caactctcaa ttgccgttgc actatcgcct tgccgctgtg tggggcgccc
     6121 acgaaggatc gcttctgctg tggacgttca ttctcacggt ctggatggtg gccgttacgc
     6181 tcttctcgcg ccagttgccg ttgccgatgc gtgcgcggat tcttggcgtg atggggtttg
     6241 tcagtgtggg ctttctcttg ttcatgttga ccgtttcgag cccgttcgag cgactgattc
     6301 ccgctgcggc cgacgggcgc gatttgaatc cgttgctgca agaccccggc atggtgatgc
     6361 acccgcccat gctctatatg ggctatgtcg gcttctccgt ggcttttgcc tttgccatcg
     6421 ccgcgttgct cggcgggaat cttgatgccg cgtgggctcg ctggtcacgc ccctggacga
     6481 ccgtcgcctg gtgcttcctc acgatcggca tcgcgctggg cagcggatgg gcctactacg
     6541 aattgggctg gggcggttgg tggttttggg atccggtcga gaatgcgtcg ttcatgccct
     6601 ggctcgccgg cacggcgttg attcactcgc tggccgtcac cgataaacgc ggcagtttca
     6661 aagtttggac cgtgctgcta gccatcctgg cgttctcgct cagcctggtc gggacgttcc
     6721 tggttcgatc cggcgtgctc acgtccgtgc atgcgttcgc gaccgatccg aaacggggtc
     6781 tgttcatcct ggcgtacttg gcgatcgtca tcggaggatc actgacgctc tatgcctggc
     6841 gggcgccgcg ggtcggacta ggagggcgat tcgccttcgt ctcccgtgaa tcgatgttgc
     6901 tggtgaataa cgtgttgctc gtggtcgcga ccggctcggt tttgttgggc acgctctatc
     6961 ccctgttcct ggacgcactc ggaatgggga aactctccgt agggccaccc tatttcgata
     7021 cggtgtttgt accgctcatc acgccggccc tctttctcat gggcatcggc ccattggcgc
     7081 agtggaaaca ggcctcgctg cctgatttgg ccatcagggt tcgctgggca ttcgccgtca
     7141 gtgtcgcgac cgcactcctg cttcctttgg tggtcggcaa gtggacaccg ctcgtgagtc
     7201 tcgggctctt gctcgccatg tggattctcg cgaccgcggt cctcaatgtc cgtgaacgcc
     7261 tgacgcacgt cactgtgaac agcctctgga gtggactcgc cagtttcccg cgctcgtatt
     7321 ggggcatgct cctggcccat tgtggcatcg cggtcttcat cattggcgtc acattcgtca
     7381 agggattcga gaccgaaaag gacctgcgga tgaacgtcgg cgaaacagcc acgatcggcg
     7441 gctaccagtt ccgtttcgat ggggtgcagg aaatcaaagg gccgaactat cgggccgggc
     7501 gagggatctt cttcgtgacg aaaggtggcc ggccgattac cgagatgtac ccggagaagc
     7561 ggttctatcc cgttcagaat caggcgatga cggaagcggc gatcgataca ggcttcctgc
     7621 gcgatctgta tgtgtcactc ggggagcccc tcgacggtgg tgcctggagc gtgcggctgt
     7681 accacaagcc cttcatcgac tggatctggg gcgggtgttt gctgatggcc ttcggcggcg
     7741 tcctggcgat cagcgaccgt cgctatcgac tgtcgtggcg gaagcaggat gagacggctc
     7801 ccgtattggc gactccgaga ccatcggagg tggcatgaaa cggtttctga ttcctttggg
     7861 atcgtttgcc gtgatcatgg gatttctcgc ggcggggctg accctcaatc cgcgggaagt
     7921 cccctcgccg ttcatcggca agcccgcacc ggactttacg ctgcctcagc tgcatgatcc
     7981 ggagaaagtc ttctcgccca aggacctcgc agggcaggtg tggttattga atgtctgggc
     8041 ctcctggtgc agcggctgca aggacgaaca tccggtgctg atgcagctgg ctcagagcgg
     8101 atcagtaccg atttacggca tggactataa ggaccagcgg gcagaggccc tctcgtggct
     8161 caagcggcag gggaatccct accccctgag cgcgatggac gaagccggtc gaattggaat
     8221 cgactacgga gtgtatggag ttcctgagac ttttgtgatc gataagaagg ggatgatccg
     8281 atacaaacag atcggtccat tggatcaaga ggcattggat cggaaagtgt tgccgctcgt
     8341 gagggagtta cagaaccaat gaggttcttc gtgtttttgc tctgcttgct accaggcgtt
     8401 gtcttcgcgc aggaggcgcg tccactcggc gagaatccgg ccgttgaggc acgcctcaag
     8461 caactggccg tcgagctgcg ttgtctggtt tgtcaaaacc agacgctggc ggattccaac
     8521 gcaccattgg ccgaagacct gcgtcgggaa gtgcgcgaga tgatcgcgag gaatatgacc
     8581 gatgaccaga tcgtcgactt cctggtgacg cgctacggag atttcgtccg gtaccgccct
     8641 ccgttcaaag taacgacatt gctcttgtgg gtaggcccgt ttctcctgct cgccgccggg
     8701 gccacggtgt tgatgctgtc attgcgccgc cgagcgcaga agaccgaaga aggaatgctt
     8761 tcgcaggagg aacaggcacg ggccgcacga ctcttggaag gcaagggagg atcaagaaca
     8821 tgagcatcct gtttgtcctg attgcggcaa cattgacgat cggagtgatg ctgcttttgg
     8881 tgctcccctt actccgaccg ccctctcagg cggacgcccg cgagaccgac aagcggctct
     8941 cggtctatcg gcagcaattg gctgagttgg aacaggaccg tgcagcttgt accctcacac
     9001 aagagcagtt tgagcactct cgtcgggaac tcgaacgccg cgtgcttgag gaaaccacaa
     9061 gcgattctgt gccaccgggg ccgtcccggt ggcaattgtc cagcaggaca gtagccttgg
     9121 cgctgatcgt gacgatcccg atcgcagtat tcctgctcta ctggacgctc ggcaacccgt
     9181 tagccctcat ccatcccggc gcgcaagcga aggagacggg agggaattcg gaatacgctc
     9241 accaatcgtc aaccagtctc gataccttga cggaccgact caagcaacgc atggagcaga
     9301 gtccgaacga tggagcaggg tgggcgttgc tggcgcgttc ctacgtggag ctcggacgtc
     9361 acggcgatgc tgtcacagca tatgaaaagg ctgtcgctct gattccggag gatgcccagt
     9421 tgttggtcga ctatgccgac gcgttggcga tggcccgagg tagaaagttg gaaggcaagc
     9481 cggaagattt gatcgcacgc gcgctgaagc tcgacccgcg gaacgtgaag ggtctcatgc
     9541 tggccgggac aatagcctac gatcgaaaag attttccgac cgcgctcaag cattgggagc
     9601 aggcacagaa gggcttaccc cctgagacag agcctgagct ggtccaggaa ttggccgcca
     9661 acattaacga agttcgagga ctgctcggcc tcgcagttgg tggcgggaag atggcgagca
     9721 tgccgacaga cgtcccgacg tcaaccaagc aggttcgcca gaatcttgcg atcagcggca
     9781 tggtcacctt ggcaccgagc ctcgctggaa agggttctcc cgttgataca ttattcgtgt
     9841 tcgcgcgcga gacgaatggt ccgccgatgc cggtttcaat tgtgcgggcg acaaaacaag
     9901 atttgccttt tacgttccgg cttgatgact caaccagtcc catgccatcg agaaaactgt
     9961 cggacgtagg cacggtggtg atcgtggccc gtctgtcgaa gtcaggcgaa gccatgccga
    10021 aaagcggcga tgtgcagggg atgagtcagc cggtgaagcc gggcgcgaag ggcatcaacg
    10081 tcatattgga tcgtgaaatc ccctagttga gagctgttcc tatgtccatg tccgcaatca
    10141 tcactctact gtatgttctt cctcttataa ttgccctcat cctctactac cgccgacatc
    10201 gcaaaaggga aacccattac cgcacccgct tggccgagat ggtcaagtca gggattacgg
    10261 agccgctgac cctgcatccg gttatcgaca cgaacaaatg cattggctcg ggtgcgtgcg
    10321 tgagggcctg tccggagcac tcgctgggga tcatccgagg gaaagctgtc ttggtggagc
    10381 cggaccattg tatcggacat ggcgcttgtg aaaccgcctg cccggtcgaa gcgatcacgc
    10441 tcgtgtttgg gacagagaag cgcggcattg acatcccgca agtgaagccg actttcgaaa
    10501 ccaacgtgcc aggcattttt atcgccggcg aactcggcgg aatgggactg attcgaaaag
    10561 gagtcgatca gggaaagcga gctatcgaat cgattcgaaa aaacagaagc aagggaaccg
    10621 agttggatgt ggtcatcgtc ggagccggtc ctgcgggact ttctgcttcg ttggcggcca
    10681 aggaagcagg attgcggttt gtgaccatcg aacaagaaga tggcgtcgga gggtctgttt
    10741 atcattaccc gcgacgaaaa ctgaccatga cggcgccaat gaaactgccg attgtcggca
    10801 agctcaacaa gcatcagatc agcaaggaag aactgctgga gttttggaat cgcatcatca
    10861 ggcaaacggg cctcaccatc aatttcacgg agcggatgga atcgatcaca aaagccggcc
    10921 gcgggtttgt cgtgaagacg tctaaagcaa cctacaaggc gaccaatgtc ttgctcgcga
    10981 tcggacggcg cgggacgccg cgcaagctgg gcgtgccggg cgaagaacaa tccaaagtcg
    11041 tctacagtct catcgactca gagcaatacc gaggacagca tgtgctcgta gtgggtggcg
    11101 gagacagtgc ggcggaggct gcgctcgcta tcgcagctga gcgcgggacg gtcgtcacgg
    11161 tttcctgtcg cggagatgag atatttacca gaccgaaaga gcggaaccgg aggcagttga
    11221 agatgctatc cgatcaaggg aaggtgaagg tatttctgca cgcgaacgtg aagcagatcg
    11281 ggataaacca tgtgacgctt gatcgcgagg ggacggttat tgaactgaag aacgatgcgg
    11341 tgatcgtctg cgccggaggg accctgccga ccccgatgct aaaggaaatc ggagtgatgg
    11401 tgagcaccca ttacgggacg ccggttgctc gctgaaagca atcgcgcgag agcaagacga
    11461 agttgagcag tgcagagggg cgtcatgatc aaggaggtga caaaccccat gctctctttc
    11521 tcacaacgga tatataaaag accatccggc cgcaattttg cccgatatgt ccaccttctt
    11581 tcagccagtg ccttgttggt ctcgtgcgtt tcgattcgcg tcgaatacct cgcagatgaa
    11641 acctatcctt caagagagca tgcagcttcg gtcgagtggc tctcggcaga accgtccgag
    11701 ccctacatca agattgccag gattgttgct gatagtacga gtggaagcca ggaaacgttg
    11761 cgccaggcca tccttgatcg cgcccgccgt ctgggggctg atgcggtgat cgatgaaaag
    11821 gctgcaacgg tgtcctctcg cgcaccgtca cccaattacg agcgaggagt cttgggccca
    11881 gcaggggcct cctttgatct ctatgggtat ggtcattcca ctccctacgc tccagctccc
    11941 taccttttgt cccaaggagc cacggaccag ccaagactgg acgagtactt gtcgggaatg
    12001 gcgatacggt tcagcggcag cccacgacgg tgagccccag cgagatcgta accgattgga
    12061 aaattacgaa acgggaaaga tggatttcga gttcagaagg gacagcgcaa gattactctt
    12121 ggaatcgagc ttcccgggcg tacagaagat actgttccga ttctaaatct agattggacc
    12181 ggtgcgatat gtcagagagc ttatggacaa cgatttgtcg aaacagcgaa gagttgtatc
    12241 agggtggggt catgtcccga cagcgatcga tccgacgtat ggtgcacgtt tcactagtca
    12301 tcgagagaga tgtggctagc ttgcaagacg ccatttccta aggagccatg gatttcaact
    12361 tgggcaccca taacgaggtc gttgatggtg cctccctcga tgatcgtcga agtcgtcgtc
    12421 atgacgtgcg tgttgttgac aaagaaatct cccggcgtgt tgacttgcag aatgactcct
    12481 tccacttctg cctcagaatc gtcttcaacg ccaagcgtga gcggtttaac aagcgtcgcg
    12541 gttaatttcg caccgtgagg gccgggacct ccctggctcg attgatcgcc gcggacctgc
    12601 acgacgatgt tattccatgt tgcactacca ggagaaggca tttcactcgt atcggcagac
    12661 gaataattca cgacgagggc gccgatctcg aacgttttct gcttcacatc gtgattcttg
    12721 acggaaccct ggatctcata gtgaggaggg tcggcttgct tcatgatcag ggtcgcgaga
    12781 atttcgccat ctccggtcac aaagccgctg acttcaacga gatccaatcc tggtattaaa
    12841 ctatgaacgg attggcctgt gatactcggg tcgatcaccg ttttctgatt gagacggaca
    12901 agttgtccaa ggacaaatag gcttaacccg tcagacgcca ccgactgtac gattccttcg
    12961 acggtctctt cataggtaat cgtgtcggcg atacgggcca gcggttgtcc agatgaatat
    13021 tctttactga ttctgccatc gaccaggacg accataccga ttttgagggt attttggggt
    13081 ccacaaggct cgtcatctat gcaatagatc gtgttgctgt tgtcgtattc tgtacctgaa
    13141 acaaacacgc tgccgaactt ggtaaccggg cctgaggaga ctgtcttcac agaaccagtc
    13201 ccaccgatgc cgccgccgac ttgagggcca gggccttgag ggccagggcc gcacgacacc
    13261 aacgacaggg gcagagctag aaaaacgagg aacttctgct ttgcggtctg tctcatgagg
    13321 aggggctcac ttagtccgaa tccaatcgtt cctcgaaatg ataaattcca agccccacgc
    13381 gcacacggcc gcttcccttg gaacccgggt tcacatcacg atctcgatgc gcaagccatc
    13441 gatcaatcgt ctccaagagc tcctgtcctt tagcggccgc gaggatccga aattccttgg
    13501 cggcctcgac gggcacattg tcgtacatga cctttcgttg aaagcgaggc ttggacggtt
    13561 tctgatacac gttgtggtcg atggt
//
LOCUS       DATDNW010000056         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_961812, whole genome shotgun sequence.
ACCESSION   DATDNW010000056 DATDNW010000000
VERSION     DATDNW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961812"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..1128
                     /locus_tag="VI359_01485"
     CDS             577..1128
                     /locus_tag="VI359_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972952.1"
                     /translation="MAEYPRFRHVCRSAVLMAVWYALTPNSGHTDEVVKPPDQILSDE
                     LQLLKEEESVDAGSQAVRPISFSSSEPYVMTEEDIRNSGAEDLPALFRRILSLALSQM
                     AGSNINRGDRVDDQPIANNLLVMVDGHSSYIDASDALTSMNIPVTLPEIKRLEMWKES
                     TSPSQEANTYHLVIKISTKTPGK"
     gene            1145..3478
                     /locus_tag="VI359_01490"
     CDS             1145..3478
                     /locus_tag="VI359_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6972953.1"
                     /translation="MTVSTSRHTVLVVEDTQDIVMGLRDLLEHDGYAVTVAGTCASAI
                     EYVRTQRVNAILLDLTLPDGDGIEVMKAAHSLDPTLPVIIVTANTSPDKTVGSLTRGA
                     FNYLIKPYNKEELRQTLRRAIGVKELAVKVERVQHLLSESEDRFRSLVESATDAIIVA
                     NGGGFIIAWNRSASTLFGYTIEEAIGQPLTILMPERYRQAHEQGLARIASTGRGRVMG
                     SIVELHGLKKDGTEFPIELSLATWKNTNTTYYSGIIRDISERKKAERVLQESQERFRQ
                     LAENIVEVFWMTDPAKQQMLYISSGYEKIWGRSCESLYASPQSWLEAIHPEDRLRVLD
                     AATQKQVAGTYDEEYRIVRPDGTIRWIYDRAFPIRDSSGIVYRIVGFAEDITDHKRIQ
                     ESLRTSEERLELVIQGSSDGYWDAKILPGESWNSPRTPVWWSPQVKAMLGYTDEEFPD
                     VLESWASRLHPEDVDRVFAALRDHIDRRIPYDVEYRLRAKSGDYRWFRARGQAMWDDS
                     GRFVRMSGSLQCVTDRKRAEEALRRHQQLLQDVIDNTTAVIYVKYADGRYLLTNRRFE
                     QLFNLTTSEIVGHTDHEIFPQDFADAFRANDLAVLERNTTVEYEEYAPHPDGPHTYIS
                     INFPLCDHTGKPYATCGISTDITERKRIEDELRSNDVRARLALTDASGGIWDWDLQSN
                     QLFWSPQVDTLLGLAPGSFSGTEREWLALVYPDDREAVLLVKRRALERHRTNVAFDHR
                     VQWPDGTLHRLTWAGHIVRDQKGEAVRVLGTVREQKS"
     gene            3529..>4155
                     /locus_tag="VI359_01495"
     CDS             3529..>4155
                     /locus_tag="VI359_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY6972954.1"
                     /translation="MRISDYTSRPFSVRPVAIAPSIAGLAVLLGLLSLLVSLATAADI
                     AILKSSEIAAYDDAVKGFMATAPAGATYTEYNLRGDLELGKKLAKKIRSSDTSLVVAV
                     GLKAALAAKLELGDIPTVFMMVLDPQKHQLNGANMTGTLLEIPVDRQLKLLRTFLPTK
                     HRLGILYDPSKSSSRIKDAEPHAAANDFQLQGFPVASEKDVPQQLRALL"
BASE COUNT          930 a         1234 c         1125 g          866 t
ORIGIN      
        1 ggaaccgcgc tgcctctgcg gatgggcata gcccgacact gaaacccgaa taccgacgat
       61 tcttatgcaa gacatcatct cttctagcaa cggcatcaat ggcgtgggtc gtccgaagcc
      121 ggctctgacc tgcacccagg ctaccaggca gcgggtcacc gtctcccttc ccacgtcatt
      181 ggtggagcga cttcgcaatg cagtgtattg gacagggcac cacaccctcg cgcaaatcat
      241 cgtggattcg cttgaagcta ccgtcaccgc gatggagcat gacaacggag ggccctttcc
      301 tggacggctg gctccgctca agtcagggag acgtccccgc aagccgctac cttcccacct
      361 cattgcgacg gactcttcac ccggagtacg cgtagccagt tgaccgcttg ctcagctgtt
      421 gagcggtctc tcttcgcgct tcgagtggag acaccgtctt gtattcatac ccgctcacga
      481 gtaggctttt gcacgagtgt tcatgaccag catatctttt gcagtaatcg tggctgtcct
      541 ccctgagcat ctgcttttca gccgtggagg gcacccatgg cagaataccc acgatttcga
      601 catgtgtgtc gttcggccgt cttgatggcg gtatggtacg ccctgacacc caactcgggt
      661 cacaccgatg aagtggtcaa accgcccgat caaatcctca gcgatgagct gcaactgctt
      721 aaggaagagg aaagcgtcga tgctgggtcg caggcggtta gaccgatctc cttctcgtct
      781 tcagagccgt atgtgatgac cgaggaagac atcaggaatt ccggagccga agatcttcca
      841 gcgttgtttc ggaggatttt gagtttagcc ctttcgcaga tggcaggctc gaacattaac
      901 cgcggcgatc gcgtcgacga tcaacccatc gcgaacaacc ttctcgtcat ggtggatgga
      961 cactcgagct acatcgatgc gtctgatgct ctcacatcga tgaatattcc tgtgaccttg
     1021 ccggagatca aacgtcttga aatgtggaaa gagtcgacct cccctagcca agaagcgaac
     1081 acgtaccacc ttgtgattaa gatcagcaca aagacgccgg ggaagtgagg tgctggaggt
     1141 tcccatgact gtttcaacgt cacggcatac ggtcctcgtg gtggaagata cccaggacat
     1201 tgtgatgggc ttaagggatt tactcgaaca tgacggctat gccgtcaccg tggcgggcac
     1261 ctgtgcgagc gccattgaat atgtccggac ccaacgcgtg aacgccatcc tgctggattt
     1321 gacattgccg gatggagatg ggatcgaggt catgaaggcg gcgcacagtc tggaccccac
     1381 cctccccgtc attatcgtca ccgccaacac ctctccggac aagacagttg gctccctgac
     1441 ccgtggggcg tttaattacc tgatcaaacc ctacaataag gaagagcttc gacaaacgtt
     1501 acggcgcgcc atcggggtca aggaactcgc cgtcaaggtc gagcgggtgc aacacctgct
     1561 ttctgaaagc gaagataggt tccgttccct cgtggaatcc gccacggatg ccatcatcgt
     1621 ggctaatgga ggcggattca tcattgcctg gaatcgttct gcctcgacgc tcttcggcta
     1681 caccattgaa gaagcaatcg ggcagccgct gacgatcctc atgccagaac gctaccgcca
     1741 ggcacacgag caaggtctcg ctcgcattgc ctccacgggc agaggccgag tgatggggtc
     1801 catcgtcgaa ctgcacgggc ttaagaagga cggcacagaa tttcccattg agctctccct
     1861 ggccacctgg aaaaatacga acactaccta ctatagcggg atcatccgag atatctcgga
     1921 gcgcaaaaag gcggaacgcg tgttacagga aagccaggag cggttccgcc agctggcaga
     1981 aaatatcgtg gaagtctttt ggatgactga tcccgcgaaa cagcagatgc tctatatcag
     2041 ttcgggttat gaaaagatct ggggacgatc ctgcgaaagt ctatatgcct cgccccaatc
     2101 atggctcgag gcgattcatc ccgaagatcg tctccgtgtc ctcgatgcag ccacacagaa
     2161 acaggtggct gggacctacg acgaggagta tcgcatcgta cgaccggatg gaacaatccg
     2221 atggatctac gaccgcgcgt tcccaatccg cgattcctct ggaatcgtct atcgcatcgt
     2281 cggttttgcc gaagacatca cagaccataa acgcatccag gaatccttgc gcaccagcga
     2341 agaacgtctg gaactcgtga tccaagggtc cagtgacggg tactgggatg cgaagatcct
     2401 cccgggcgaa tcgtggaact ccccacgcac gccagtctgg tggtcgcctc aagtcaaagc
     2461 catgctgggc tataccgacg aagaatttcc tgacgtcttg gaaagctggg cctctcggct
     2521 tcatcccgag gatgttgatc gcgtctttgc cgccctcagg gaccatatcg atcgtcgcat
     2581 cccctatgat gtggaatatc gcctccgcgc caaatccgga gactatcgat ggttccgcgc
     2641 gcgaggccag gccatgtggg atgactcggg ccggttcgtc cgtatgtccg gctctctcca
     2701 gtgcgtgaca gatcgcaagc gtgcggaaga agcgcttcgc cgtcaccagc aactgctgca
     2761 ggacgtgatc gacaacacaa ccgccgtgat ctatgtgaaa tatgccgacg gccggtatct
     2821 cctcacaaac cgtcggtttg aacagttatt caacctgacg acaagcgaga ttgttggcca
     2881 taccgatcac gagattttcc cccaagattt cgctgacgcg tttcgggcca acgacctagc
     2941 ggtgctggaa cggaacacca cggtggaata tgaagaatat gcccctcacc cggatggccc
     3001 ccacacctat atctcaatca actttcccct ctgcgaccat accggcaaac cctatgcgac
     3061 ctgcggcatc tctactgaca ttaccgaacg aaagcgcatc gaagacgaac ttcgttccaa
     3121 tgacgtgcga gcccgccttg ctctgaccga tgcatcggga gggatttggg actgggatct
     3181 tcagtccaat caactcttct ggtcgcctca ggtcgacacc ttgctcggcc ttgcaccggg
     3241 ctcgttctcc ggcactgaac gagagtggct cgcgctcgtg tatccagatg atcgcgaagc
     3301 ggtcctgctc gtcaagcgtc gggcattgga gcggcatcgc acgaacgtgg cgtttgacca
     3361 ccgcgttcag tggccggacg gcaccctcca tcgcctcacg tgggccggcc acatcgttcg
     3421 cgatcagaaa ggagaagcgg tgcgtgtact gggaacggtc cgtgaacaga aatcatgaag
     3481 cggatgactc gctgcaacac gacatcgtgc tgtacggatc cggcagtcat gagaatctct
     3541 gactacacca gcagaccctt cagcgtacgg cctgtggcga ttgcaccatc catcgccgga
     3601 ctggcagtct tgctcggctt gttaagcctg cttgtttccc tcgccacggc cgccgatatt
     3661 gccatcctga aatcttccga gatcgctgcc tacgacgatg ccgtcaaagg attcatggca
     3721 accgcccccg ccggagcgac gtatacagaa tacaatctgc gaggggattt ggaactcgga
     3781 aagaaactgg ccaaaaagat ccgctcctcg gacacctccc tggtcgtggc cgtcggcctg
     3841 aaagcggcgc tggcggccaa actcgaatta ggcgacatcc cgaccgtctt catgatggtc
     3901 ttggatccgc agaagcatca gttgaacggg gcgaacatga ccggtacgtt gctggagatt
     3961 ccggtcgacc gtcagctgaa acttctccgc accttcctgc cgacgaagca tcggcttggc
     4021 attctctatg atcccagcaa gtcctcttcc cggatcaaag atgcggagcc ccatgccgcc
     4081 gcgaacgact ttcaacttca aggattcccg gttgcgagcg agaaagacgt gccgcagcaa
     4141 ttgcgtgccc tcctc
//
LOCUS       DATDNW010000057         4000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_418405, whole genome shotgun sequence.
ACCESSION   DATDNW010000057 DATDNW010000000
VERSION     DATDNW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418405"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..468)
                     /locus_tag="VI359_01500"
     CDS             complement(181..468)
                     /locus_tag="VI359_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEY6972955.1"
                     /translation="MSIIVGLCTVELFIPDSQSLKDRRQVLLSLKDRLRDKFNLSVAE
                     VGEQDLWQKAVLGLACVANETRYVNQVLEQALNVIRATPAVEIVQSRVDVL"
     gene            complement(488..3022)
                     /gene="infB"
                     /locus_tag="VI359_01505"
     CDS             complement(488..3022)
                     /gene="infB"
                     /locus_tag="VI359_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY6972956.1"
                     /translation="MRVYELAKQLGMENRDLIPELKRMGIAVASHSSALEEEAVHKVL
                     AKLGHKSKPSTKPGGHVAESGDDRAERGKDAGHGAKAGGGHGTPTVEEPKPDKRRILI
                     KRKKAEEGGAEEGAATVVGTESSSLIAPSAESPSSLTITPPVEISVSPHVEPMTRPSG
                     AAQPSAQTSPGTSAMVPTVEPVLGKKKSVAFEAIEAEGLKDKLKKGRKLARQRDDEQV
                     VVREDAARWQDLRAIPVQPRRDDRAKHAHHSTLAEITKPRKKSVKLTPGMTVKEFAEM
                     IGQRPADIVRKLMDMGQMLTFNQPMNTDAAAIIAEESGVKLDVAVEKAGEELLESLVQ
                     TEGEGNLEARPPVVTIMGHVDHGKTSLLDAIRETKVAEGEAGGITQHIGAYTVTVRGK
                     QVTFLDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAIHHAKAASVSLIVA
                     INKIDKPGANPDRVRNALSEHGLISEAWGGETIMVEVSAKQKTGLDQLLEMILLQAEI
                     LELKADPHRLAKGTVVEAKLERGRGPLATVLVQGGTLHVGDAFVVGAFSGRVRALLSH
                     TGAKVQEAGPSIPVGVVGLPGVPAAGDVFQVVKDERVAREIAEDRARKQRAAELAGQG
                     KVSLDDLFAKIQEGSVKELALVIKADVQGSAEALAAGIEKLPTDVVKLRVIHSGVGGI
                     TESDVLLAAASNAIIIGFNIRPEPKAATLAEQEGVDIRLYTIIYDAMADIKAAMEGLL
                     EPTLKERTVGRAEVRQVFTVSKAGTVAGSYVLDGTITRQSAGVRVIRDNVVVYQGRLS
                     SLRRFKDDVREVQQGYECGIGIENFNDIKTGDILEVYAIDKIAAKL"
     gene            complement(3046..>4000)
                     /gene="nusA"
                     /locus_tag="VI359_01510"
     CDS             complement(3046..>4000)
                     /gene="nusA"
                     /locus_tag="VI359_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY6972957.1"
                     /translation="KAEISLKEARQYDSEAEVGDEIGSLIEMDELGRIAAQTAKQVIF
                     QKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNYLVDLGKTEAILPIQEQIPRETYR
                     RGDRVKAMLLEVRRTPKDVQVILSRSHPQFVAKLFELEVPEVTEKIVEIKSVVREPGD
                     RTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWTSDPRVFIAEALNP
                     ATIEKVGIDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWKIDIISATEYEKEK
                     AERDKEIKAALAEEAEAQRQQEEARKQEREAEEGSQASDAG"
BASE COUNT          731 a         1301 c         1016 g          952 t
ORIGIN      
        1 acgtctgtga ccgtcaccga acggacacgc gggtccttga tctttcgcat gaggatatct
       61 gccacctcca tgcgaatctg atccgctacg cgatcggccc gcttgtagcc tgtcttcatc
      121 atcgctctca agctcgagcg gcctcgacaa tccactcgcc actcgcggcg atggtcgtta
      181 tcacaacacg tcgacgcgag actgaacaat ctccacggct ggagttgcgc ggatcacgtt
      241 caaggcctgc tccaagactt gattcacata gcgtgtctcg ttagcgacac acgcaagtcc
      301 gagcaccgcc ttctgccaca aatcctgctc gccgacctcg gcaactgaca gattaaactt
      361 gtcacgcagc cgatccttga gactcaaaag cacttggcgc cggtccttca acgactgact
      421 gtccggaatg aacagctcga ccgtacagag tccaacgatg atactcacag tccagcgggc
      481 tcacgtctca aagtttcgcg gcaatcttat cgatcgcgta cacttcgagg atatcccctg
      541 tcttgatgtc gttgaaattc tcaatcccga ttccacattc atagccctgt tgcacttcgc
      601 gaacgtcgtc cttgaaccgc cgcaacgacg acaacctgcc ttgatacaca acgacgttat
      661 cgcgaatgac ccggaccccc gcgctttgcc gcgtgatggt cccgtccaac acgtaagagc
      721 cagccacggt cccggctttg gagacagtga acacttgacg tacctcggcc cgaccgaccg
      781 ttcgctcctt gagcgtcggc tcgagtagtc cctccatcgc cgccttaatg tcagccatcg
      841 catcatagat gatggtatac aggcggatat ccacaccttc ctgctcggca agcgtggcgg
      901 ctttgggttc aggcctgata ttgaacccga tgatgatcgc atttgatgcg gcggcaagaa
      961 gcacgtcgga ttccgtgatc ccgccgacac cgctgtgaat gacacggagc ttcaccacgt
     1021 ccgtcggcag tttctctatg cctgccgcca gcgcttccgc ggatccttgt acatcggcct
     1081 tgatcaccaa agctaattcc ttgaccgatc cctcctggat ctttgcgaag agatcgtcca
     1141 gcgacacctt tccctgcccc gcgagctcag ccgcccgttg cttccgggct cggtcttctg
     1201 cgatctcgcg cgcgacacgt tcatccttga ccacctggaa gacatcgccg gctgcgggca
     1261 cacccggtaa cccgacaacc cccacgggaa ttgacgggcc ggcttcctgc actttcgcgc
     1321 ccgtgtgtga cagcaatgcc cgcactcgtc cgctgaaagc cccgacgaca aatgcatcgc
     1381 cgacatgaag tgtgccgcct tggaccagca ccgttgccag cggtcctcgc ccccgctcaa
     1441 gcttcgcctc gaccaccgtt cccttggcta atcgatgagg atcggctttc aattccaaaa
     1501 tttcggcttg gagcaaaatc atttccaaaa gttggtctag gcccgtcttc tgcttggcgg
     1561 acacctccac catgattgtc tcgccgcccc aggcttcgga gataagccca tgttccgaga
     1621 gtgcatttct gactcgatcc ggattggctc caggcttgtc gattttattg atagcgacga
     1681 tgagggatac cgaagcggct ttcgcgtgat ggatggcttc gatggtttgc ggcatcacgc
     1741 cgtcatcggc cgcgacgacc aggacgacga tatcggtgac ttttgctccc cgagcccgca
     1801 tagctgtgaa ggcctcatgg ccgggggtgt ccaggaacgt gacctgcttg cctcgtaccg
     1861 tgacagtgta ggcgccgatg tgttgggtaa tcccgccggc ttctccttcc gcaaccttgg
     1921 tttcgcggat cgcatccagc agcgaggtct taccgtgatc gacatgaccc atgatcgtca
     1981 cgaccggcgg cctggcctcc aggttacctt caccctctgt ttggaccaag gattccaata
     2041 gctcttcacc ggctttttcg accgccacat cgagtttcac cccactctct tcggcaatga
     2101 tggcggcggc atccgtgttc atcggctggt tgaacgtcaa catctgaccc atgtccatca
     2161 acttccggac aatgtcggcg ggacgctgac caatcatttc cgcaaactcc tttaccgtca
     2221 tgcctggtgt gagcttgacg cttttctttc tcggcttggt aatctccgcc agcgtactgt
     2281 ggtgcgcgtg ctttgcgcga tcatcgcgac gcggctgaac gggaatcgcg cggagatctt
     2341 gccaacgtgc ggcgtcttca cgaacaacta cttgctcgtc atcccgttgt cgggccagct
     2401 tgcggccctt tttcagcttg tccttgagtc cctcagcctc gatggcttca aatgcgacgc
     2461 tcttcttttt gccgagaacc ggttcgacgg tgggcaccat cgcggacgtg ccaggggaag
     2521 tctgggcaga gggctgagcc gcaccggagg gtcgggtcat tggctctaca tgcggagaga
     2581 cggatatctc gacaggaggt gttatcgtga gggaacttgg tgattctgcc gatggggcga
     2641 tgagactcga cgattcagtc ccgacaacag tcgccgctcc ctcttcagcg ccaccttcct
     2701 ccgctttctt tcgcttgatg aggatgcgcc gtttgtccgg cttcggctcc tcgactgtcg
     2761 gagtgccatg cccgcctccc gctttcgcgc catggccggc atccttccca cgttcggcac
     2821 gatcatcacc agactcagcc acgtggccgc ccggcttggt cgacggcttt gatttgtgcc
     2881 ccagcttcgc gaggactttg tggaccgcct cctcttcgag ggcgctgctg tgagaggcga
     2941 cggcaatacc catccgcttc aattcgggga tgagatcacg gttctccatc ccaagctgct
     3001 tggccaattc atacacgcgc atagagagtc gacacccgtt tcacgttatc cggcatccga
     3061 cgcctgagat ccttcttctg cttcccgctc ctgctttctg gcttcttcct gctggcgctg
     3121 agcttcggct tcttccgcca acgcggcttt gatttcctta tctcgctccg ctttttcctt
     3181 ctcatattcc gtggcgctaa tgatgtcgat tttccatccg gtcagccgag cggccaagcg
     3241 aacattctgg ccgttcttcc cgattgccag cgacagctga gaatccgcca ctacaacaag
     3301 cgctgacttc ttctcttcat cgatcccgac tttttcgatt gtggcagggt tcaacgcctc
     3361 ggcgatgaac acgcgcggat cagatgtcca ggtaatgatg tcgatctttt ctccgcgcag
     3421 ctcgcggacc acggcttgca cccgggagcc cttgatgcca acacaggctc cgacgggatc
     3481 gaccgctttc tcgcgggacg tcacggcgat ctttgtccgg tcgcccggct ctcgcaccac
     3541 cgatttgatc tccacgattt tttccgtaac ctccggcact tccagttcga agagcttcgc
     3601 cacgaattgc ggatgacttc gagaaagaat cacctgcaca tctttcggcg ttcgtcggac
     3661 ttccaagagc atggccttca cccgatcgcc gcgacgatac gtttctcgcg ggatctgctc
     3721 ttgaatcggg aggatggcct cggtcttgcc caagtccacc aggtagttcc ggcgttccat
     3781 accgagaata atgccgttta ccaaatcacc ctgacgagtg gagtattcct tctgcaccgc
     3841 ctcccactcc gcctcacgga ctttctgaaa gatcacctgc ttcgccgttt gcgccgcgat
     3901 ccggcccaat tcgtccattt caatcaagga cccgatctca tctcccacct cggcttcgct
     3961 atcatactgg cgggcttctt tgagagaaat ctcggctttg
//
LOCUS       DATDNW010000058         2845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1235911, whole genome shotgun sequence.
ACCESSION   DATDNW010000058 DATDNW010000000
VERSION     DATDNW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235911"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2039)
                     /gene="uvrA"
                     /locus_tag="VI359_01515"
     CDS             complement(<1..2039)
                     /gene="uvrA"
                     /locus_tag="VI359_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HEY6972958.1"
                     /translation="MSGSIIIKGAREHNLKNIDVTIPRDKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLFARVGRPYCFQCGEEITAQTVQQMVDAISVLPEGSKFQVLAPIVRGRK
                     GEYRKELLDMRRAGYVRARVDGELVDLGDDIMLDKQKKHTIEIVVDRLVMKPGDALMR
                     RLADSVETSLKLAGGLVGVLRDDGKVHLYSEKLACIKCGVSYPEITPRVFSFNSPPGA
                     CPACDGIGYVMTPGCPEEEDFTLLEPCDVCKGARLRPESLSIKLAKQSIAEVTHFSVR
                     AAADFFVSLKLTDRELVIAHRILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMMV
                     ADHLLDMGPGAGSQGGQVIAQGTPQEVMGNADSLTGQYLRGVQMVSLPRRQRKPKSML
                     SIIGAKKHNLKGITAKIPLGLFTCVTGVSGSGKSTLVLEVLFHSLSQLLYQKKPKIDG
                     CKELTGVDALDKVIDIDQSPIGRTPRSNPATYTGLFSFIRDLYSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLEIHHKGKSIA"
     gene            2111..>2845
                     /locus_tag="VI359_01520"
     CDS             2111..>2845
                     /locus_tag="VI359_01520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEY6972959.1"
                     /translation="MLSVEESVTPDQRQSSSAAHTISTWSGSAREQAVQRMFTAIARV
                     YDLNNTLLSFGLHHRWKKITASYVPVTTAGRALDVGAGTADLALLIEPRMGENGLVVA
                     SDLNHAMLAEGQKKIAARALKSRITCLEANAEQLGFPDRTFDAVTTGFCMRNVGNLQQ
                     AFAEVYRVLRPGGRFVCLEFSRPVMGWLRKLYDWYSFRLLPWIGTKVARDTTGVYEYL
                     PASIRTFPDQERLCQALRDAGFRQVEY"
BASE COUNT          573 a          845 c          819 g          608 t
ORIGIN      
        1 gcgatgctct tccctttgtg atgaatctcc agggtctcgc ggttatagcg ttgtccctta
       61 caaacctcgc aggtcacgta cacatccggc aggaaatgca tctcgatctt gatgaggccg
      121 tcgccctggc atgcttcaca gcgcccgccc ttgacattga agctgtagcg gcccggcttg
      181 tacccccgca cccgcgattc cggcaaattc gaatagagat cccggatgaa actaaacagg
      241 cccgtgtagg tcgcgggatt ggaacggggc gtccggccga tcggcgattg atcgatatcg
      301 atcaccttgt cgagcgcgtc cacgcccgtc aattctttgc acccgtcgat tttcggcttc
      361 ttctgataca acagttgcga aagagaatgg aacaatactt cgagcacaag cgtgcttttg
      421 ccggatccag agaccccggt cacacaggtg aagagtccca gcggaatctt ggccgtgatg
      481 cctttcagat tgtgcttctt ggctccaatg atcgacagca tgctctttgg cttgcgttgg
      541 cgacggggca ggctgaccat ctggacgccc cggagatatt ggccggtcag tgaatcggcg
      601 ttccccatca cctcctgtgg agtgccttgc gcaatgacct gcccgccctg cgatcccgca
      661 ccgggtccca tatccaacag atggtccgcc accatcatcg tctccgcatc gtgctcgacc
      721 acaaccacgg tgttgccgag atcacggagc cggagcaatg tctgcaagag acgtcgattg
      781 tcccgctgat gaagtccgat cgacggttca tcgagaatgt agagcacgcc cactaggccg
      841 gaaccgattt gggtcgcgag gcggatgcgt tggccttcgc cgcccgacaa cgtcgccgca
      901 gcacgatcta aggtcagata gtcgagaccg acgttgacca ggaatcccag ccgctcgcgg
      961 atttccttga gaatgcgatg cgcgatgacc aactcgcggt cggtgagctt gagcgagaca
     1021 aaaaaatcgg ctgcagcccg gaccgaaaaa tgcgtgactt ccgcaatcga ctgtttcgca
     1081 agtttgattg acaaactttc cggcctgagc cgcgcgccct tgcagacgtc gcagggttcc
     1141 agcaacgtga agtcttcctc ctccggacag cccggcgtca tgacatagcc gatcccgtcg
     1201 caggccgggc aggcaccggg tgggctgttg aaggagaaca ctcggggggt gatttccgga
     1261 tagctgaccc cgcatttgat gcaggcgagc ttttcgctgt agagatgcac tttgccgtcg
     1321 tctctcaaca caccgaccaa ccctccggct aatttcaaag acgtttccac tgaatcggcc
     1381 aaccgccgca tcagcgcgtc acccggtttc atcaccagtc ggtcgaccac gatctcgatc
     1441 gtatgttttt tctgtttatc gagcatgatg tcgtcgccca gatcgaccag ctcaccatcg
     1501 actctcgctc ggacatagcc ggcccgtcgc atatccagaa gttctttgcg gtattcgccc
     1561 ttcctgccgc ggacgattgg ggcgagcact tggaacttac tcccctcggg caatacgctg
     1621 atcgcatcga ccatttgttg gacggtttgc gcggtgatct cctcgccaca ttggaaacaa
     1681 tagggacgtc cgacgcgcgc gaatagaagc cgaaggtagt cgtaaatctc cgtcacggtg
     1741 ccgacggtgg atcgaggatt gtggctggtg ctcttttgtt cgatggagat cgccggggag
     1801 agtccctcga tcgaatcgac gtcgggtttc cccatctgct caagaaattg acgcgcgtag
     1861 gccgacagag attcgacgta tcgccgctgc ccctcggcgt agatcgtgtc gaacgcgagc
     1921 gaggatttgc ctgaaccgct caggccggtg atgacgacga gcttgtcgcg tggaatcgtg
     1981 acgtcgatat tcttgagatt gtgctcgcgg gcacccttga tgatgatcga gccggacata
     2041 tgacgcagga ttatatcatc cgccgccgtt gccttgacaa aggcggaaac cgggtgctac
     2101 gacggctggc atgctctctg tggaggaatc tgtgacgccg gatcagcgtc agtcgtcgtc
     2161 ggccgctcac acgatctcga cctggtccgg ctcggcgcgc gaacaggccg tgcagcggat
     2221 gttcaccgcc atcgcacggg tctatgatct gaacaatacg ctcctcagct tcggtctgca
     2281 tcaccggtgg aaaaagatca ccgcatcgta tgtgccggtc accacggccg gacgggccct
     2341 cgacgtcgga gccggcacgg cggacctcgc gctcctcatt gaacctcgca tgggtgaaaa
     2401 cggactggtc gtcgcttccg atttgaatca cgcgatgctc gccgagggac aaaaaaagat
     2461 tgcggcgcgt gcgttgaaga gtcgcataac gtgcctcgaa gccaacgcag aacagttggg
     2521 attccctgac cggacgttcg atgccgtgac cacgggattc tgcatgcgca acgtggggaa
     2581 cctccagcag gcatttgcag aagtctatcg agttctgaga ccaggaggcc gtttcgtgtg
     2641 cctggagttt tcccggccgg tgatgggctg gctccggaag ctctacgact ggtattcttt
     2701 ccgactcctg ccctggatcg gtacgaaagt cgcgcgggac acgaccgggg tgtatgaata
     2761 cttaccggct tcaatccgga cattccccga tcaagaacga ctctgccaag ccctgcgtga
     2821 tgcaggcttc cgccaggtgg agtat
//
LOCUS       DATDNW010000059        12442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_688934, whole genome shotgun sequence.
ACCESSION   DATDNW010000059 DATDNW010000000
VERSION     DATDNW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688934"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..767
                     /locus_tag="VI359_01525"
     CDS             252..767
                     /locus_tag="VI359_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972960.1"
                     /translation="MLRFPWATSVWLLLGVIVVVPAVGGGCSGKTIQYPDDHERYRRI
                     DQAVESLRHSYERKNASKMADLMVPTEQIDRLQRDVENDFESFYSIELGFTIERIMID
                     GDDVDVFVHWQGLWKKNADDPGLRQRGHTRLQWVGTLSILLRGIQGDSPFGMKVRQTI
                     TDNSPPSSKKR"
     gene            771..2414
                     /gene="nadB"
                     /locus_tag="VI359_01530"
     CDS             771..2414
                     /gene="nadB"
                     /locus_tag="VI359_01530"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY6972961.1"
                     /translation="MPSRIARSPLQADFLVIGSGVAGLRAAVELAPKGRVLVLTKGHP
                     LQSSSIHAQGGVAVAMSEEDDVSIHLTDTLKAGHGLCRKEAVRVLVEEGPERIQELIK
                     WGARFDKAGGKFAFAREAAHSRSRILRARGDATGNEMVRALIAQVNRQKNVQRLDYHF
                     TVDLAVEGGRCCGAVVLDESSGRQFILPARAVVLSTGGAGQIFARTTNPPNATGDGIA
                     MALRAGAVLQDMEFIQFHPTALYLPSSPPFLLSEAMRGEGGQLRNSKGELFMHRYHPM
                     GALAPRDIVSRAIWAEMAATKARHVYLDVTHLGADFVKRRFPTIYATCLRYDIDITEE
                     WIPVSPSAHYMMGGVWTDVNGATTVPGLFAAGEVACSGVHGANRLASNSLLEGLVFGA
                     RAAEAAVAFAGRHNVPSLSSYEAAIRPGQFGTLEDAEKLRSSLRRTMWGQVGIIRSGE
                     SLIRACAQLSRWAQIVSQPFASRAALEVKNMVQVAQCVAEAALWRENSVGAHYRSDFP
                     QAKRVGWQQHSRVSMVDGVSERKVRKARGLVLVLPGHAG"
     gene            complement(2350..3051)
                     /locus_tag="VI359_01535"
     CDS             complement(2350..3051)
                     /locus_tag="VI359_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6972962.1"
                     /translation="MNSTTSLRLVQLGCRGITALIMVSLASWSLPLSDLQAEVYQYVD
                     AAGTIALTNVPSDVRYRKVELDSNPLRIVLPERELAPVIARHSRQQQLHPALIRAVIK
                     AESNFDPHAISRAGAIGLMQLMPQTAVRLDVRDLYDPDDNVGGGTKYLRQLLDRFHGN
                     LPLALAAYNAGENVVDRYQALPPIDETRQYVRKVLRYYRTFLTRDGFITERPVTQRVP
                     AAPTQDLAPSAPSVR"
     gene            complement(3154..4038)
                     /locus_tag="VI359_01540"
     CDS             complement(3154..4038)
                     /locus_tag="VI359_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY6972963.1"
                     /translation="MWIFLNNRFVKQQHAVVSVYDHGFLYGDGVYETIRSYGSRIFMR
                     DQHLARLRRSADAIGLAMPIPEKDWPGLLHEAMERNGVGNDRRDAYLRITISRGVGEI
                     GLDPALCPVPTVVIMAKPLHPPSRQQYEQGVSIIVAQTRRNLPSALSPHIKATNFLNN
                     ILAKREAIAAGVFDSVLLNWQDHVAECTVSNLFWVKGGTISTPSLDCGILDGITRDIV
                     LTLAREAGLPVQEGHFGIEALHQADECFLTNTSMELMPVTTFEHRPVGSGKPGPITHQ
                     LQTQFVVNRRRFLESDHN"
     gene            complement(4047..5525)
                     /gene="pabB"
                     /locus_tag="VI359_01545"
     CDS             complement(4047..5525)
                     /gene="pabB"
                     /locus_tag="VI359_01545"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase, component I"
                     /protein_id="HEY6972964.1"
                     /translation="MTHFTQPSFLYGTPQPLVLTLEDQQADPFDLYCRIASPSQPSFL
                     LDSGTAVARGLHYSFLGCSPSAILTGHKEQSSYQTREGREAMLVDPFSYLGKMMADAH
                     ICKTDGLPPFIGGAVGYLSYDLVRQFETLPSLAADDLSLPDLQFAFFDVIAAVEHQTN
                     RVHLIYCPSGERFLGEPREKLYREGLDRLAAFAAQLMGTPPEATPSPIIRQVSFHPEQ
                     TQAAYMDRVRRCQDYIAAGDIYQANLSHRFTVAINDMPENSRDRLMACQDLYGRLRRV
                     NPSPFSGLLHFDNMALVSCSPERLVRLRGQLADTRPIAGTRPRGRDEADDRRLVNELL
                     SNEKERAEHLMLVDLERNDLGRVCQFGTVQVDEFMTVEQYSHVSHIVSQVSGTLRPDA
                     TAFDLIKAMFPGGTITGVPKIRCMEIIDELEPVRRGPYTGSFGYLSWNGNLDLNITIR
                     TLVLCHTTGYLQVGAGIVADSNPSREYEETLHKAQAFFSSLQ"
     gene            5672..5893
                     /locus_tag="VI359_01550"
     CDS             5672..5893
                     /locus_tag="VI359_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY6972965.1"
                     /translation="MPPSQDPFYAGLGQAVRIGTELLAALIVGGGLGWAVDTYLLDSG
                     PWAMVGGLIVGAIAGVRNAYRSAQQWPKS"
     gene            5924..6673
                     /locus_tag="VI359_01555"
     CDS             5924..6673
                     /locus_tag="VI359_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY6972966.1"
                     /translation="MEESPLHPFELHNYIPISLGGLDISINKAVVMMWVVVALVTLLM
                     VMAGSARKLVPGKLQSLAEILVDFIRGIIIDTMGKDGMRFFPLVATLFLFILFCNLLG
                     LIPGAYTVTSQIIVTAVFACTVYGLSLLMGFLLHGAKFLGILVPPGTPAWLLPLMVPI
                     ELVSQLARPISLAVRLFANMTAGHVILGVLFGLAISGGVLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLTCVYLGDAFHLHGQDGHAH"
     gene            6710..6940
                     /gene="atpE"
                     /locus_tag="VI359_01560"
     CDS             6710..6940
                     /gene="atpE"
                     /locus_tag="VI359_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY6972967.1"
                     /translation="MDSAAAALLGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            7031..7537
                     /gene="atpF"
                     /locus_tag="VI359_01565"
     CDS             7031..7537
                     /gene="atpF"
                     /locus_tag="VI359_01565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY6972968.1"
                     /translation="MPQFESHFFSSLIFWEVLSFAILLFVLYKFAFPGILSALEEREK
                     KIRDSLDQAERFRTEAEHKLKEYEAKLNAASKEADGILATAKERAQRILEENEQRLTA
                     DAERIKGDATREIDQERRKAIQEIRTQTTDLALMVAEKVVERSLTDADHRRLADEALA
                     ALAKSYHQ"
     gene            complement(7563..8372)
                     /gene="larE"
                     /locus_tag="VI359_01570"
     CDS             complement(7563..8372)
                     /gene="larE"
                     /locus_tag="VI359_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY6972969.1"
                     /translation="MPTDTLQRKLENLRHDLTDMRSVLVAYSGGIDSAFVLKIAHEQL
                     GSCAIGVTAVSPTFPASELEIATQVAAEIGARHEIVRTDQLAIPAFVQNDASRCFHCK
                     TDLYKLLDELREPRASRWIVDGTNLDDLGDDRPGIKAAREWNVRSPLVEAGFSKSDIR
                     TLAKDLGLSNWDKPAAACLSSRIPRGIPITVDKLRRVEGAEEVLLAEGFRQVRVRDHG
                     EIARIEISQEEFARLNDPERRGLISSRLRELGFRFVCVDLQGYRPGGISVS"
     gene            complement(8405..9769)
                     /locus_tag="VI359_01575"
     CDS             complement(8405..9769)
                     /locus_tag="VI359_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6972970.1"
                     /translation="MLSSTTHPARIPLLPVIAIAAACFWLWGSSLSLAADIAPSKFVT
                     TNGITVLVLEQHFLPIVEIHALIKAGSAQDPPEKAGVANLTASLLDEGTTTRSSKQLA
                     EQIDFVGGSLEAKASEDFTTASARVLKKDVELGFTLLADILQHPAFQKQEFERIRTQL
                     LGEMASDNDDPGHVAMKAFNQLVFHGHPYRWPVNGTEETLTKVTLADVQAFYAKEYQP
                     SQVILTIVGDVTVEQATTLVQTHFGAWKKGGAFPRNIKDPSPIKRKTVQLIEKDLTQS
                     TIILGHGGIQRAHPDFYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSHFDARLL
                     PGSFWINLQTRTEATNQAIAGVLTELKNIREAPVSDQELAEAKSFLMGSFPLRFDTTA
                     KLAQVLAQVEFYGLGFEYFSQYPKWIERVTKEDVQRVAKQYLDPQRYALVVVGNIAKA
                     KVKH"
     gene            complement(9782..11188)
                     /locus_tag="VI359_01580"
     CDS             complement(9782..11188)
                     /locus_tag="VI359_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6972971.1"
                     /translation="MHRAFLHDVIMTFLLPPHKTTRLIVAALVVACSLFLLHPVLAAD
                     PREFVLANGMKVLLIEVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTTKY
                     PKGSFSRLIRKNGGMDNAFTSQDFTAYFENLAADRVDLALELEADRMQGLLFDMNELK
                     TEREVVKEERRLRTEDDPQGALVEALFAQAFLSHPYHWPVIGWFGDLDAMTLDDLQRH
                     YDTFYSPNNATLIIVGDIKADTLLATIKQLFEPIPRGPEPKPLTTIEPEQKGERRFLL
                     KREAQVPFVMMGYRVPNYTSDDSYALDILESILQRGKSSRLYQSLVYDQKSALAVGAE
                     YSLMQTDPGLFYFYALVSPGQKVEAVEEALNKEIKRLQTDPPTDQELQRAKNQVEAAR
                     VFEQDSNFRHAMLLGQAETVGAGWRKVDQFLERIRAVTAKDVQRVARQYLTDDNRTVG
                     SLIPVPNKQPDAPAAAIH"
     gene            complement(11190..12296)
                     /gene="rlmN"
                     /locus_tag="VI359_01585"
     CDS             complement(11190..12296)
                     /gene="rlmN"
                     /locus_tag="VI359_01585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY6972972.1"
                     /translation="MSSTHDQPLPPPTAVNLLALDERGVGHIVQSLGWPKYRTGQVLR
                     WLYRRGVRTIDQMTDLSLTERRTLQTVATIRRITASQVFRSTDHTRKLVLTLEDGVNV
                     EAVLIPDNDRLTLCVSTQVGCTLDCGFCLTGTMGLTRNLKPHEIVEQVLTAREHLDEG
                     ERITNLVFMGMGEPLANLDSLSDAIRRLTNQTWGLKWSPRRITVSTAGLASRLKDVAP
                     LGVNLAISLNATTEEQRARLMPAANGLASLKTLLAACRRYPLPPNRRLTFEYVLLAGM
                     NDRRDDAQRLAKLLRGLSCKVNLIPFNEFPGNSFRRPLDQEVLAFQAILKRACIDTFI
                     RKSRGRDVLGACGQLGTLSSHQPTPALTQIQPRC"
BASE COUNT         2658 a         3285 c         3691 g         2808 t
ORIGIN      
        1 ttggcgccgg cgcagcttct tatacttatg cttgcgcatt ttcttgcggc gtttcttgag
       61 gacactcgac attcggtcta tctcctacgg aacgcagtct aaaatcttca ctaagaaatt
      121 gcaagccagc gaaggaattg gttcctgagc gatgaagcgg acgagactct gtggacggaa
      181 cgggatgaac gaacggtgga gaaatgaaag cccgcttgac cctattcagc acccgttcct
      241 atactgcacc gatgttacgt ttcccatggg ctacatctgt ctggctgttg ctcggcgtga
      301 tcgtcgtcgt gcccgcggtc ggaggcgggt gcagcggaaa gaccattcaa tatcccgatg
      361 accatgaacg ctaccgtcgg attgatcagg cggttgaatc gttgcgccac tcctacgaga
      421 ggaagaatgc ctccaagatg gccgacctga tggttccgac ggaacaaatc gatcggctgc
      481 agcgggatgt ggaaaatgac tttgagtcgt tctactcaat agaattgggg ttcacaatcg
      541 agcggatcat gatcgatggc gacgacgtcg atgtgttcgt tcactggcag ggactctgga
      601 aaaagaatgc agatgatcct gggctccgac agcgcggtca tacccgtctt cagtgggtcg
      661 gtactctgtc gatccttctg agagggattc agggtgattc cccctttggc atgaaagtcc
      721 ggcagaccat caccgacaat tccccgcctt cgtcaaagaa gagataaccc atgccgtccc
      781 gcattgctcg ctccccgctg caggccgatt tcctcgtgat cggtagcggt gttgcaggtc
      841 tccgcgccgc ggtagaactc gctcccaaag gacgtgtcct tgtcctgacg aaaggacacc
      901 ccttacaaag cagttcgatc catgcccaag gcggcgtcgc ggtggcaatg agtgaagaag
      961 acgatgtgtc gattcatctg accgatacgc tcaaggccgg acacggcctt tgccggaaag
     1021 aagcggtgcg ggtgttggtt gaagaaggtc ctgaacggat tcaagaattg atcaaatggg
     1081 gggcccgatt tgataaagcc ggaggcaagt tcgcgtttgc ccgcgaagct gcgcatagtc
     1141 gcagccgaat tcttcgcgcg cgtggtgatg caacgggtaa cgagatggtt cgcgcgctga
     1201 ttgcacaagt caaccgccaa aagaacgttc agcggctcga ctatcacttt actgtcgatc
     1261 ttgcggttga ggggggacgc tgttgcggcg cagtggtgct cgacgaaagc tcagggcgcc
     1321 agtttatatt gccggctcgc gccgtggtcc tcagcaccgg tggggcggga cagatcttcg
     1381 ctcggacgac gaatccaccg aatgcaacgg gcgacggtat cgccatggcg cttcgagcag
     1441 gagcggtgct tcaagatatg gagtttatcc aattccatcc tacggctctg tatcttccct
     1501 cgagccctcc ctttctcctg tcggaagcca tgcgcggaga aggcggtcag ctacggaaca
     1561 gcaagggtga attattcatg caccggtatc atccgatggg cgcacttgcc ccgcgggaca
     1621 tcgtctcgcg tgccatatgg gcggagatgg cggcgacaaa ggcacgacat gtctatctcg
     1681 acgtgaccca tttaggcgcg gatttcgtga aacggcgttt ccctacaatc tatgcgacct
     1741 gcctccgcta cgacatcgat atcaccgagg aatggattcc cgtctcaccc agcgcgcatt
     1801 acatgatggg cggagtctgg acggatgtga acggcgcaac gactgttccg ggtttgtttg
     1861 ccgctggcga agtggcttgc agcggtgtgc acggtgcgaa caggctggcg agcaattcgc
     1921 tcctggaagg gctcgtgttc ggcgctcggg cggcagaggc cgctgtagct ttcgccggtc
     1981 gacacaatgt gccatcgctc tcatcgtatg aggcggccat tcgtcccgga cagttcggca
     2041 cgctcgagga tgccgaaaag ttgcgcagct cgttgcggcg cacgatgtgg ggacaggtcg
     2101 gtattatccg ttctggcgag tctctgattc gagcttgtgc gcagttgtca cgctgggcgc
     2161 aaatcgtctc tcagccgttt gcgagtcggg ccgcgttgga agtgaagaat atggtgcaag
     2221 tagcgcagtg tgttgctgaa gcagccttgt ggcgtgagaa cagcgtcgga gcccattacc
     2281 ggtcggactt tccgcaggcc aagcgcgtgg gctggcaaca gcacagtcgg gtttcgatgg
     2341 ttgatggggt cagcgaacgg aaggtgcgga aggcgcgagg tcttgtgttg gtgctgccgg
     2401 gacacgctgg gtaaccggtc gttcggtgat gaagccgtcg cgcgttagga acgtccggta
     2461 ataacgcaga actttgcgaa catattgacg agtctcatcg atcggtggca gagcttgata
     2521 gcggtcgacc acattttctc ctgcgttata ggcggcgagc gcgagtggaa gatttccgtg
     2581 aaaccggtcc aacaattggc gcagatattt cgtgcctccc cccacattgt cgtccgggtc
     2641 gtagaggtcc cgcacgtcca gccgtactgc cgtttgtggc atgagttgca tcaagccgat
     2701 cgcgccagct cgcgagatcg cgtgagggtc gaagttcgac tcggccttga ttacggcacg
     2761 gatcaaggcg ggatgtaatt gctgttgtcg agaatggcgt gcgatcacgg gtgccaattc
     2821 ccgctcaggc aacacgatac gaagagggtt cgagtccagt tcaactttcc gataccgcac
     2881 atctgacggc acgttggtca gcgcgatggt tccagccgca tctacgtact ggtagacctc
     2941 ggcttgaaga tcggagaggg gaagactcca actggccaac gagaccatga tcaaggccgt
     3001 gatgcccctg catccgagct gtacgagtct gagagaagtg gttgaattca tggttccacg
     3061 ataccagtct agggtgccct gtcaagaaaa tgcacgacct gtgccggttg gaacattcga
     3121 taagctcgcg tgaaatatgg ggtcaagacg accttagttg tggtccgatt cgagaaatcg
     3181 gcggcgattg actacaaatt gcgtctgaag ctgatgggta atggggccag gttttccgct
     3241 gccgaccggc ctatgttcaa aggtggtgac cggcatcagt tccatactgg tattagtgag
     3301 aaaacactcg tcagcctgat gcagagcctc gattccgaaa tgtccttcct ggactggaag
     3361 gccggcctcg cgcgccagtg tcagtacgat gtcacgcgtg atgccgtcca agatgccgca
     3421 atccagagac ggtgtcgaaa tggttccacc tttgacccaa aagaggttgc tgacagtgca
     3481 ttctgccacg tgatcctgcc aattcagcaa cacgctatcg aacacgcccg cggcaatggc
     3541 ttctcgtttg gcaaggatgt tgttcaggaa gttcgtcgcc ttgatgtgtg gcgagagcgc
     3601 gctcggtaag tttcgtctgg tctgagcgac gattatgctg acgccctgct cgtactgttg
     3661 gcgggaagga ggatgaagcg gtttggccat aatcacgacc gtcgggactg gacacagcgc
     3721 cgggtccaag ccgatctccc caactcctcg ggaaatggtg attcgcagat aggcatcgcg
     3781 gcggtcattg cccacaccgt tccgctccat cgcctcatgc aagagaccgg gccagtcttt
     3841 ctcgggaatg ggcatcgcca gcccgatcgc atcggccgaa cgacgaagtc gagccagatg
     3901 ctggtcacgc atgaagatcc ggcttccata cgaccgtatg gtttcgtaca ctccgtcgcc
     3961 gtagagaaaa ccgtggtcgt agacagaaac gaccgcatgc tgttgtttca caaaccggtt
     4021 attgagaaat atccacataa ctccatttat tgaagcgagc tgaagaaggc ctgagccttg
     4081 tgcagggtct cttcgtactc tcttgaggga ttggaatccg caactatgcc ggctccgact
     4141 tgcaggtatc ctgttgtatg acacaacaca agagtccgaa tcgtgatatt gagatcgagg
     4201 ttaccgttcc agctcaggta accgaacgag ccggtataag ggccgcggcg caccggctcc
     4261 agctcatcaa taatctccat acaccggatt ttcgggacgc cggtaatggt tccaccgggg
     4321 aacatggctt tgatcaggtc gaacgctgtt gcatccggcc gcaacgtgcc gctgacctgg
     4381 gatacgatgt ggcttacatg cgagtactgt tcgacggtca tgaactcatc gacctgcact
     4441 gtgccgaatt ggcagacacg ccctaggtca ttacgctcaa gatcgaccaa catcaggtgt
     4501 tccgctcgtt ccttctcgtt cgacaggagc tcattgacga gacgccgatc gtcagcctcg
     4561 tctcggcccc gcggtctcgt cccggcaatc gggcgggtat cggccaattg acctcgaagc
     4621 cgaaccagtc gctcgggcga gcaactcacc agagccatgt tatcgaagtg caagagccct
     4681 gagaacggag aaggattgac gcgtcggagc cgaccgtaca ggtcttggca ggccatgaga
     4741 cggtcacggc tgttctcggg catatcattg attgccacag taaaccgatg agacagattg
     4801 gcttggtaga tgtctcctgc cgcaatatag tcctggcaac gccgcacgcg atccatatag
     4861 gcagcctgag tctgttccgg atgaaacgac acttgtcgga tgatcggtga aggtgttgcc
     4921 tccggtggcg ttcccatcaa ctgtgccgcg aacgcggcca accggtctaa cccctcgcgg
     4981 tagagttttt ctcgaggttc tccaagaaat cgttcccccg agggacaata tatgaggtgg
     5041 acacgattgg tttggtgttc gaccgccgcg atcacgtcaa aaaaagcaaa ttgcaggtcc
     5101 gggagcgata aatcatccgc ggcgagggac ggaagggttt caaattgtcg gacgaggtca
     5161 taactcagat agccgaccgc tccaccgatg aatggtggga gtccatcggt cttacagata
     5221 tgagcatcgg ccatcatttt gccaagatag ctgaaaggat cgactaacat ggcctctcgt
     5281 ccttcccgcg tttgatacga cgattgctcc ttgtggcctg tcaggatcgc ggagggacta
     5341 cagcccagaa aggagtagtg gagcccgcga gcaacagccg taccgctgtc gagcaagaac
     5401 gaaggctgtg aaggagaggc gattcggcag tacaaatcaa atggatcggc ctgctggtct
     5461 tcgagcgtga gaaccagcgg ctgtggagtg ccgtaaagga atgagggttg agtgaaatgc
     5521 gtcatggtgg atctgagcct gagagaccaa tgagtgacac tcactatact ccagggattt
     5581 gtcttctgtc acagcggcgg cggcttgaca acctcacgag gattctgctt gaattgcgcg
     5641 cccgccatcc agggagaccc cgggccacaa catgccccct tcacaagatc cgttttatgc
     5701 ggggcttggc caagcggttc ggatcggaac cgaactgttg gctgcgctga ttgttggagg
     5761 agggttgggt tgggccgttg atacatacct tctggatagc ggtccatggg ccatggtggg
     5821 gggcttgatc gtaggggcga ttgccggggt ccgaaacgcc tatcgctcgg cgcagcaatg
     5881 gccgaagagc taagaagggc gttgcgcgtt cggagaaagt ctgatggaag aaagtccgtt
     5941 acatcccttc gaactgcata actacatccc gatttcattg ggcgggctcg acatttccat
     6001 taataaagcg gtcgtcatga tgtgggtggt ggttgccctg gtgacgctgt tgatggtgat
     6061 ggccggttcc gcgcggaagc tggttccagg aaagttgcag agtctggcgg agatcttggt
     6121 cgattttatc cgtggcatca tcatagacac gatgggtaaa gatgggatgc ggtttttccc
     6181 actcgtcgcc acgttgttcc tgttcattct cttttgcaat cttctcggtc tgattcccgg
     6241 tgcctacacg gtcacgagcc agattatcgt gaccgctgtc ttcgcctgta cggtgtatgg
     6301 actaagtctt ttgatgggct ttctgctgca cggggcaaag ttcctcggca tcctcgtacc
     6361 cccgggaaca ccggcttggt tgctgccttt gatggtccca attgagttgg tcagtcaatt
     6421 ggccaggcca atctcactgg ccgtccgctt gtttgcgaac atgacggcgg gccacgttat
     6481 cctaggcgtg ctctttgggc tggcgatcag cgggggggtg ctcattgggt ggctgccctt
     6541 tgccttcacg attgccatga acgggttgga agtcggtatc gcgtttattc aagcctatat
     6601 tttcaccgtg ttgacctgcg tctatttggg agacgcgttt catctgcatg gacaagacgg
     6661 tcacgcgcat taatgcgtgt gaaaacagac aagggaggag taagacacaa tggattcagc
     6721 agccgcagca ctgttgggca tgggcctcgc cgccgcagga ttcgccggag ccggggtagg
     6781 gatcgggtat atcttcggca agatgatcga ggcggtcgca cgtcaacctg aggcggaagg
     6841 ccgcgtcggc aaatacatgt ggatcgggtt cgcgctggtc gaagccatcg cgctgtatgg
     6901 cttggttatc gcgttcatca tcatgggtct acgaaaatag gagcaggcga aagactcgag
     6961 gtgattggct ggaggaagac tgcgaattcc tcttgcccct cgcagcgggc cctacgcctg
     7021 attgcttgat atgccgcagt ttgagtcgca ttttttttca tcgctgattt tctgggaggt
     7081 gttgtcgttc gccattctcc tctttgtgct ctacaagttc gcctttcccg gaattttgag
     7141 cgcgttggaa gaacgcgaga aaaagatcag ggacagtctc gatcaggctg agcgttttcg
     7201 gactgaggcg gaacacaaac tcaaggagta tgaagccaag ttgaatgctg cttccaaaga
     7261 agccgacggc atcttggcca ctgcgaagga acgagctcag cggatactgg aggagaacga
     7321 acaacgcttg acggctgacg ctgaacggat caagggcgat gcgacacgtg aaatcgacca
     7381 ggagcggcga aaggccattc aggagatccg cacacagaca accgacctag ccctgatggt
     7441 cgccgaaaaa gtagtggagc gcagcttgac cgatgctgac catcgcaggt tggcggatga
     7501 ggcactagca gcgctcgcaa agtcctatca tcagtaggtt ccacaatcgc agatacggag
     7561 ggttagctca cactaattcc gcccggtctg tagccttgta aatccacaca gacgaatcga
     7621 aatcccagct cccggaggcg actgctgatg agtccgcggc gttcggggtc attcagccga
     7681 gcgaattcct cctggctgat ctcgatccgg gcgatctcac cgtgatcccg gactcggacc
     7741 tggcgaaatc cttccgcgag caggacctct tcggcccctt caacacgtct aagtttgtcg
     7801 accgtgatcg ggattccgcg tgggattctc gaagacagac aagccgccgc cggcttgtcc
     7861 caattggaga ggccgaggtc tttcgccagc gtccggatgt cggatttcga aaaccccgct
     7921 tcaactaaag ggctacggac gttccattca cgcgctgcct tgattcctgg tcgatcgtcc
     7981 cccaggtcgt cgaggttggt cccatctaca atccatcggc tggctcgtgg ttcccggagc
     8041 tcgtcaagga gcttatagag gtcggtcttg cagtggaaac agcggcttgc gtcgttttga
     8101 acgaacgcag gaatcgcgag ctgatcggtc cggacgatct catggcgggc cccgatttct
     8161 gccgccactt gtgtggcgat ctcgagttcg ctagccggaa atgttggcga aactgcggtg
     8221 acgccgatgg cgcagctccc gagttgctcg tgagcgattt tcaatacgaa tgcactatca
     8281 atcccaccgg agtacgccac caggacagag cgcatgtcgg tgaggtcgtg acgaagattc
     8341 tcgagtttgc gttgcagtgt gtcagtcggc atggtcgatt tgttttgcac gggacccgga
     8401 ctaactagtg tttgactttc gcctttgcga tattgccgac gacgacgagg gcgtagcgtt
     8461 gcggatccag gtactgtttc gcgacgcgct gaacatcttc tttcgtaact cgttcgatcc
     8521 atttcggata ctgtgagaaa tactcgaatc ccaggccata gaattcaacc tgcgccagga
     8581 cttgagccag tttcgcggtt gtatcgaacc gaagtggaaa acttcccatc aagaatgatt
     8641 tcgcctcggc caattcctgg tctgagaccg gagcttcacg aatattcttg agttcggtca
     8701 ggacgccagc aatggcttgg ttggtcgctt cggtccgcgt ttgcaaattg atccagaatg
     8761 accctggcaa cagtcgcgca tcgaaatgac tcatgatgcc ataggcgagt ccctgtttgt
     8821 ctcgaatcga gtccatcagc cgagatgaaa acccaccggc gccaaggatg tagttcatga
     8881 cggtcacggc ataaaaatcc ggatgagccc gctggatacc accgtgcccc aagataatgg
     8941 tggattgagt tagatctttc tcgatcagct ggacggtttt tcgtttgatc ggcgaggggt
     9001 ccttgatgtt gcgcggaaac gcccctcctt ttttccatgc gccgaaatgg gtttgcacta
     9061 atgtggtggc ttgttccacc gtcacgtcgc cgacgatcgt cagaataact tgcgaaggtt
     9121 gatattcctt ggcgtaaaac gcttggacgt cagcgagcgt aaccttggtg agcgtctctt
     9181 ctgttccatt taccggccag cgatagggat gtccgtgaaa caccagctgg ttgaacgcct
     9241 tcatggccac atgaccggga tcgtcgttgt cactggccat ctcgccgagg agttgtgtac
     9301 gaatcctctc gaactcctgc ttttgaaagg caggatgttg aagaatatcg gcaaggaggg
     9361 tgaatccgag ctcgacgtct ttcttcagga cccgcgcgga ggccgtcgta aaatcctccg
     9421 aggctttggc ttcgagagat cctccgacga aatcgatttg ttccgccagt tgttttgaac
     9481 tcctggtcgt cgtgccttca tccaagaggc tggctgtcag gttggcaact cccgctttct
     9541 ctggcgggtc ctgtgcggac ccggctttga tgagggcatg aatctcaacg atggggagga
     9601 aatgctgttc gagcacgagc acggttattc cattcgtggt gacgaacttc gacggagcga
     9661 tgtccgctgc caggctgaga gaggatcccc agagccagaa acacgctgcg gctatggcga
     9721 tgaccggcaa gagcggaata cgtgctgggt gggtggtcga agacagcatc ctctggtgtc
     9781 cctaatgaat ggctgccgcc ggggcgtcgg gttgtttgtt tggaacagga ataagggatc
     9841 cgaccgtgcg attgtcgtcg gtcaggtact gccgcgcgac gcgttgcacg tccttcgcgg
     9901 tgacggctct gattcgctcg agaaattggt cgactttacg ccagccggca ccgaccgttt
     9961 cggcctgacc cagcaacatg gcgtgccgga aattcgaatc ctgctcaaaa acccgtgcgg
    10021 cttcaacctg attcttggct cgttggagtt cttgatccgt cggcggatca gtctggagcc
    10081 gtttgatctc cttattgagt gcctcttcca ccgcctcaac cttttgtcct ggactgacga
    10141 gagcataaaa atagaagaga cccgggtcgg tttgcatcaa gctatactcg gcgccgactg
    10201 cgagcgcgga tttctgatca tagaccaggc tctgatagag acgcgaactt ttccctcgct
    10261 gcaagatcga ttcgaggata tcgagagcat atgagtcgtc gctcgtatag ttcggcacgc
    10321 gataacccat catcacgaac ggcacttgcg cctcacgctt caacaggaac cggcgttcgc
    10381 ccttttgttc cggttctatg gtggtcagcg gcttcggttc cggtccacga gggatgggct
    10441 cgaataactg cttgatcgta gccagcagag tgtcggcttt gatgtcgccc acgatgatta
    10501 gcgtggcgtt gttgggagaa tagaatgtgt catagtgtcg ctgaaggtcg tccagtgtca
    10561 tggcatccag gtcgccgaac cagccaatca ccggccaatg ataggggtgg ctcaagaacg
    10621 cctgagcgaa cagggcttcc accagtgccc cttgaggatc gtcctctgtt cggagacggc
    10681 gctcctcctt cacaacctca cgctccgttt tcagctcgtt catatcgaag agaagcccct
    10741 gcatccgatc ggcttcgagt tccaaagcga ggtcgacgcg gtcggctgcc aggttctcaa
    10801 agtaagcggt gaagtcttga ctcgtgaacg cattatccat cccgccgttt tttcggatga
    10861 ggcgagaaaa agaacctttg ggatacttgg tcgttccctt aaacatcatg tgctcaagca
    10921 tgtgagagag tcctgcccgg cccatcactt cgtttcgcga tccgaccttg taccagacct
    10981 gcacggtcgc cactggggct ttgggcactt cgatcagcag gaccttcatc ccgttggcaa
    11041 gtacgaactc gcgagggtct gccgccaaga ccggatgaag caggaagagg gaacaagcaa
    11101 cgacgagcgc cgcgacgatg agtcgagtgg ttttgtgcgg gggaagcaag aaggtcatga
    11161 tgacgtcgtg aaggaaggca cgatgcatgc taacaacggg gttgaatttg tgtcaaggca
    11221 ggcgttggct gatgtgacga cagagttccc aactgaccgc aggcaccaag gacatcgcgt
    11281 ccacgactct tgcggatgaa ggtgtcgatg cacgcgcgtt tgaggattgc ctgaaaggcc
    11341 agcacctcct ggtctaacgg acggcgaaag ctattccccg gaaattcatt gaagggtatg
    11401 agattgacct tgcatgataa gcctcgcaag agctttgcca gtcgttgagc atcgtcccga
    11461 cggtcgttca ttccggctaa gaggacatac tcgaaggtca gtcgtcggtt cggcggcagg
    11521 ggataccggc ggcaggcggc gagtaaggtc ttcagcgagg caagtccatt ggcggcaggc
    11581 atcagacgcg cacgttgctc ctcggtcgtg gcattcagtg aaatagccaa gttgactccc
    11641 aacggagcaa cgtctttcaa tcgtgacgcg aggccggcgg tcgaaacggt gatccgcctg
    11701 ggtgaccact tgagtcccca ggtctgatta gtcagtcggc gaatagcgtc tgagagcgaa
    11761 tccaaattcg ccagtggctc gcccatgccc atgaaaacca aattcgtgat ccgctcccct
    11821 tcgtccagat gttcgcgggc tgtgagaact tgttcgacga tttcatgcgg tttcaaattt
    11881 cgtgtgagtc ccatcgtgcc ggtcaggcag aagccgcaat ccaacgtgca gccaacttgg
    11941 gtcgaaacac agagggtcaa tcgatcattg tccggaatca gaaccgcctc gacattcact
    12001 ccatcttcga gggtgagcac caatttccta gtgtggtcgg tcgatcgaaa cacttgagag
    12061 gcagtgatcc gtcggatcgt tgcaacggtt tgcagggtcc tgcgctccgt caagctgagg
    12121 tccgtcatct ggtcgattgt ccgcacccct cgccgataga gccaccggag cacctgtccc
    12181 gtgcgatact tgggccaccc gagactttgg acgatgtgac ccacaccgcg ttcatcaagg
    12241 gctaagaggt ttaccgctgt gggcggaggg agcggttggt catgagtcga agacattcat
    12301 gcagcctagc acagggtgaa aaagtggaac aagcacgaag ccgcaatcgg tttgctggca
    12361 acaaaacgag gacgctgata taaagagaga agagtcgtaa cggctagaga cggtgcacat
    12421 gtcagtttac gtaccttcca tg
//
LOCUS       DATDNW010000060         8255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_5767,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000060 DATDNW010000000
VERSION     DATDNW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5767"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1674
                     /gene="murF"
                     /locus_tag="VI359_01590"
     CDS             175..1674
                     /gene="murF"
                     /locus_tag="VI359_01590"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY6972973.1"
                     /translation="MNASTVVATEQGRDRMALFTLEELREVISVKVLGSQDPGQEKRR
                     INQISTDSRSIRPGDLFVALKGERFDGHDFVPGVLASGAVGAIVHDQYRLPEGATGGK
                     VTQGRAAPFLLGVRDTLFAYQQLATHHRSRFDIPVVAVTGSNGKTTTKDMVASVLAQR
                     WDVLKTESNFNNRIGVPTTLFRLTSRHEAAVIEMGVDQQGQTTRLCEIVRPTIGVITN
                     IGPDHLEFFGSMEGSAQAKAELLDFLPADGAAILNADDGYFDYLAARARCRVVSFGFS
                     DKADVRAVHVKTDVRHGTVFRLLLPGKVRHTIVQMKVHGTHNVTNALAAAAVGVVLNL
                     PGAVIAQGLARFRPAAMRSQVVTHHGVHIINDCYNANPASMQAAIQLLAQWSPARERI
                     AVLGDMLELGTETQRLHRDVGRFLATHNVSRLIVAGSLGREIAEGARQGGMASTRIAE
                     TADAVAAAEFLKTVVQQGDVVLVKASRGMKMEQVVQTVMGMRAIAKQAS"
     gene            1744..2820
                     /gene="mraY"
                     /locus_tag="VI359_01595"
     CDS             1744..2820
                     /gene="mraY"
                     /locus_tag="VI359_01595"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY6972974.1"
                     /translation="MLFNWLYPLHTEFSFLNVFRYQSFRIIYAAVTAFLIAFVLAPWL
                     IRKLQDIKLGQQVRDDGPQRHLAKSGTPTMGGILIIFAVVLSTLLWADLTNSYVWLVI
                     LATLGFGAIGFVDDYLKFVKARSKGLTAAQKFSAQVAVAAVIALVLYFLPSYTTKLSV
                     PFFKNVMPDLGWFYIVFAILVIVGSSNAVNLTDGLDGLAVGPVMIAALAYTIVAYVVG
                     HRLMSDYLLIPHIEGAGEIAVFTASILGASLGFLWFNTYPASVFMGDVGSLPLGAAIG
                     TAAVISKHELLLLMVGGVFVIEAVSVIFQVLSFKSRGKRIFLMAPIHHHFEMKGWEEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            2825..4234
                     /gene="murD"
                     /locus_tag="VI359_01600"
     CDS             2825..4234
                     /gene="murD"
                     /locus_tag="VI359_01600"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY6972975.1"
                     /translation="MVGVETVELAGAHVTVVGLARSGVAACRLLQTVGARVTVADRKE
                     QSELASILGTIDADRIGIRVGAGYEASLDSADLVVISPGVPYRMDALERVRRRGVKVI
                     SELELASHFLRSPIVAVTGTNGKSTTVTLIGQFLAKSGKSAFVGGNLGTALSEAASAT
                     VQASQAGRPDPYQLFVVEVSSFQLETIERFHPWIAACLNITVDHQDRYASLDEYVAAK
                     RRIFENQTASDYALVNLDDARVAAFRSGIKAKRLGFTRTQSLGSGWDGGTFLDGDRIV
                     TTVTGSRQEICRRGEIRLIGNHNIENVMAAVTYAVLCGCSLQAIRDVLATFPGLEHAL
                     EVVRERRGVRFVNDSKGTNVDATLKALESIDQPIWLIAGGRDKGGDFSRLAQALKRRA
                     KRVILIGEAASLMRAAWSGLVEMHDAATLREAVELAAREASPGDVVLLSPACASFDMF
                     ADYQDRGRQFKACVNALAA"
     gene            4268..5464
                     /gene="ftsW"
                     /locus_tag="VI359_01605"
     CDS             4268..5464
                     /gene="ftsW"
                     /locus_tag="VI359_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY6972976.1"
                     /translation="MAHRSAGTLSLPWPTANQRPAKQRVAMDHTLLVVTVTLALVGLV
                     MVFSASAIVAGNRFHDSGFFLKRQVAWLAFGFLLLHLTSRIDYTLWKKLSIPILGCMV
                     ALLVMVLVPSLGVAAKGARRWLRLGPMSVQPAELAKLVSVIYLAAYLTKKEGKITLFQ
                     GGLLPALTIIGLLSGLILLEPDLGTVVVIGLVTVGLCFLAGAKLSHLLGLALCAIPAV
                     LVLVLGSGYRRQRLLTFLAPWKDASDAGFQITQSFLAFGSGGPFGVGLGEGKQKLFFL
                     PEAHTDFVLALIGEELGLAGTATVILLFAVFVWRGFQIAARARMPFGRYLGMGITLLI
                     GIQALVNAAVVTGLLPTKGLTLPFVSYGGSSVVVSLLGVGVLLSISRDRHGGGQRGER
                     GESAHR"
     gene            5461..6588
                     /gene="murG"
                     /locus_tag="VI359_01610"
     CDS             5461..6588
                     /gene="murG"
                     /locus_tag="VI359_01610"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY6972977.1"
                     /translation="MTIVIAAGGTGGHLYPAVALARAFQRKDAATSIIFVGTARGLES
                     KVLAHEGFELVLISAKPVMGKGLIEAARGLLSLPISLWQCLKILKVRRADLVIGVGGY
                     TSPMMVLAASLIGIARVILEPNAYPGMANKAVGSFAQRVFLSFESAAAAFDRRKIRVV
                     GTPIRKEFVEQSLQKEAATPAPGQHRVLIFGGSQGARAINSAVVEGLPDLMKRHPQLS
                     LIHQTGDADFGRVAEAYRRAGISAQVTPFLYDMPSVLRSADLVVARAGAMTIAELTAC
                     GKPAILIPLPQAIYNHQTHNAKVMEAAGAAVVLPQAELTGPRLTDAITRILDDPERLR
                     TMSDRSRNMRRIDAADSIVRECYALTGDHHDGHQSVGAAGV"
     gene            6551..8047
                     /gene="murC"
                     /locus_tag="VI359_01615"
     CDS             6551..8047
                     /gene="murC"
                     /locus_tag="VI359_01615"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY6972978.1"
                     /translation="MMATSLLERRASNGIGQRETESHSSLLLGGVKRVSMFRKTQQIH
                     LVGIGGAGMSGIAEVLLTLGYKVTGSDLQASDTTRRIEELGGRVFIGHQESNVGDAQV
                     VVISSAVSAENPEVMVAKAKQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSMAAN
                     VLAQGGLDPTMVIGGKVNALGSHARLGRGDVLVAEADESDGSFLRLSPTIVAVTNLDR
                     EHLDHYGSMERINESFLEFINKVPFYGLAVLCSDDERLRALFPRIVKRYHTYGLQERE
                     GGGPDFKATDISLKQWGAEFRAHFRGRNLGPFRLSVPGIHNVSNALAAIAIGVELDVP
                     ADLIRKALAAFTGVERRFHLRGEANGIMVVDDYGHHPTEIKATLAAAKQGWEDRRLVV
                     LFQPHRYSRSRDCAEEFAHAFDQADLLFMTEIYAAGEQPIPGVSGAKLAESVRAAGHQ
                     GVTFVTQKETLPDHVLPQLKPGDLVITLGAGDIWKAGTGLLARLAPSS"
     gene            8052..>8255
                     /locus_tag="VI359_01620"
     CDS             8052..>8255
                     /locus_tag="VI359_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972979.1"
                     /translation="MNVEGRRHGSRSRAPTFEKKDLHAAVVGLKGTVSFHASLRDYTS
                     FKIGGPADVLVEPADVDDVCRLVR"
BASE COUNT         1576 a         2263 c         2654 g         1762 t
ORIGIN      
        1 gatcccggac cggcgcgagg ccatcggatc cgccatcagc gaagcccgat ccggcgacat
       61 ggtgctcatt gcggggaaag ggcacgaaga ctatcagatc atcggcacga agaagtttca
      121 tttcgacgat cgcgaagtcg cgcgtgaggc cattcaacgt ctgaggcctt gcgcgtgaac
      181 gcttctacag tggtcgcgac ggaacaagga cgagaccgca tggcgttgtt cacactcgaa
      241 gaattgcgcg aagtcatcag cgtgaaagtc ttgggaagtc aggacccggg tcaggagaaa
      301 cgtcggatca atcagatcag tacggactcg cgctcgatcc gtcccgggga tctcttcgtt
      361 gcgctcaaag gcgaacgatt cgacgggcat gatttcgttc ccggcgtgct ggccagcggc
      421 gcagtcggcg cgatcgtgca tgaccaatat cggctgccgg agggagccac tggaggcaag
      481 gtcacgcagg gacgtgccgc accgtttctt ttgggagtgc gcgacacctt gtttgcctat
      541 caacagttgg ccactcacca tcgaagccgg ttcgacattc cggttgtggc ggtcaccggc
      601 agcaacggca agacgacgac caaggatatg gtcgcgagcg tgttggcgca gcggtgggac
      661 gttctgaaga ccgagagcaa cttcaacaat cgtattggtg tgcccaccac gctctttcgg
      721 ctcacctcac gccacgaggc tgccgtgatc gagatgggcg tcgatcagca gggccaaacg
      781 accaggctct gcgagattgt gcggccgacg atcggcgtca ttaccaacat cgggccggac
      841 catctcgaat ttttcggcag catggaagga tcggcccagg ccaaggcaga actcttggac
      901 ttcttgccgg cggacggcgc cgccattctg aatgcggacg atggctattt cgactatttg
      961 gctgcgcgtg cacgatgccg ggtggtttca ttcgggttca gcgacaaagc agacgtccgc
     1021 gccgtgcatg tgaagacgga cgtgcgccac ggcaccgtgt tccggctcct gttgcccggg
     1081 aaagtgcgtc ataccatcgt gcagatgaag gtacatggca cgcacaacgt caccaatgcc
     1141 ctggctgcag cggcggtcgg ggtggtgttg aatttgcccg gcgcggtgat cgcacagggg
     1201 ctggcgcgtt tccgtcccgc ggcgatgcgg tctcaagtgg tgacgcacca cggcgtccat
     1261 atcatcaacg attgctacaa cgccaatcct gcgtcgatgc aggccgccat ccagttgttg
     1321 gcccagtgga gtccggctcg cgagcgcatc gccgtgttgg gggacatgtt ggaactcggg
     1381 acggagacgc agcggctgca tcgggacgta gggcgctttc ttgcgacgca taatgtctca
     1441 cggctcatcg tggccggatc gcttggccgc gagattgctg aaggtgcccg ccaaggtgga
     1501 atggcctcga ccagaattgc ggaaacggct gatgccgttg cagcggcgga attcctgaag
     1561 acggtcgtgc agcaaggaga tgtcgtcctg gtgaaggcct cgcgcggcat gaagatggag
     1621 caggtggttc agaccgtcat ggggatgagg gcgattgcga aacaggcgtc gtaaggaacc
     1681 tgaatccaac tgcaggagct gaaatttctt gaacgggtct cgtctaggga aagcataggc
     1741 tgaatgctct ttaactggct gtatccgctc cacactgaat tttcgttctt gaacgtcttt
     1801 cggtatcaga gcttccgcat catctatgcg gcagtgacgg cgttcttgat cgcgtttgtg
     1861 cttgctccct ggctgatccg caagctccaa gacatcaaac ttgggcagca ggtgcgcgat
     1921 gatggtcccc agcgtcatct ggccaagagc gggacgccca ccatgggcgg gattctcatc
     1981 atctttgccg tggtgctctc gacactctta tgggcggacc tcacgaacag ctatgtatgg
     2041 ttggtgatcc tggcgacctt ggggttcggg gcgattgggt tcgtggacga ttacctcaaa
     2101 ttcgtcaagg cccggtcgaa aggcctgaca gctgcgcaga agttttccgc ccaggtggcc
     2161 gtggccgcag tcattgcgct ggtgctctac tttctgccga gctatacgac caaactcagc
     2221 gtgccgttct tcaagaatgt catgccagac ctgggctggt tctacatcgt cttcgcgatc
     2281 ctcgtcatcg tcggcagctc gaacgcagtc aatctgaccg atgggctcga cggattggcg
     2341 gttggaccgg tcatgatcgc ggcgctggcc tatacgatcg tcgcctacgt ggtaggccat
     2401 cgccttatgt cggactacct cttgatcccg cacatcgaag gcgccggcga aatcgcggtc
     2461 ttcaccgcat cgattctggg ggccagcctc ggatttctct ggttcaacac ttatcccgcc
     2521 tcggtgttca tgggcgatgt cggatccctg cccctgggtg ccgcgatcgg caccgcggcg
     2581 gtcatcagca agcatgagtt gctgttattg atggtgggcg gcgtgttcgt tatcgaagcg
     2641 gtatcggtaa tttttcaagt gctgtcgttc aagtcgagag gcaagcggat ctttttaatg
     2701 gcgccgatcc atcaccattt cgagatgaag ggatgggaag agccgaaagt ggtggtccgg
     2761 ctctggatca tcgccatcct gttggcgttg ctgagtctga gcacgttgaa gttgcggtga
     2821 cggcatggtg ggagttgaga cggtggaact tgctggtgcg catgtcacgg tcgtcggtct
     2881 tgcgcgaagc ggcgtcgcag cctgtcgctt gcttcagaca gtcggggctc gggtgacggt
     2941 tgccgatcga aaagaacaaa gcgaactcgc gagcattctc ggaaccatcg atgcggaccg
     3001 gatcggcatt agggtcggcg ccgggtatga ggcgtcgctg gatagcgccg atctcgtggt
     3061 gatcagcccg ggagttcctt atcgaatgga cgcactcgaa cgtgtgcgcc gtcgaggcgt
     3121 gaaggtgatc agcgaattgg agttggcatc gcacttcctg cgaagcccga ttgttgcggt
     3181 gaccggcacg aatgggaaaa gcacgaccgt gacgctgatc ggtcaattcc tagccaagag
     3241 cggaaaatcg gcgttcgtcg gcggcaattt ggggacggcc ctcagtgagg cggcttcagc
     3301 gacggtgcag gcgtcgcaag ccggtcggcc ggatccgtat caattgttcg tcgtcgaagt
     3361 ctccagtttt cagctcgaga cgatcgagcg gttccatcct tggatcgcgg cttgtctcaa
     3421 tatcacggtc gaccaccagg atcgctacgc ctcgctggac gaatatgtcg cggcgaagcg
     3481 acgcatcttc gaaaaccaga cggcaagcga ctatgcactg gtgaatctgg atgatgcccg
     3541 agtggcggcg tttcgaagcg gcatcaaggc caagcgactg gggttcacgc ggacgcaatc
     3601 cctcggctcg ggttgggacg gcggcacgtt tctcgacggt gatcggatcg tgacgacggt
     3661 gaccggttcg cggcaggaaa tctgtcggcg cggggaaatc cgtctgatcg ggaaccataa
     3721 tatagagaat gtcatggcag cggtcaccta cgcggtgctc tgcggctgtt cgttgcaggc
     3781 gatacgcgac gtgcttgcga ccttccctgg gcttgaacat gcgttggaag tggtacggga
     3841 gcgtcgcggc gttcgatttg tcaacgactc caaaggaacg aacgtcgatg cgacgttgaa
     3901 agccttggaa agcatcgatc agccgatctg gttgatcgcc gggggacggg ataagggcgg
     3961 agatttttcg cggctcgccc aggccctcaa gcggcgtgcg aagcgcgtca ttctcatcgg
     4021 tgaggccgcg tcgctgatgc gggcggcatg gagcggactc gtcgagatgc acgacgccgc
     4081 gacgttacga gaagccgttg aactcgcggc acgggaagct tctccaggcg atgttgtctt
     4141 gttgtcgccg gcttgcgcca gtttcgacat gttcgcggac tatcaagacc gaggtcgaca
     4201 atttaaggcc tgtgtgaacg ccttggccgc gtgatcttgg atcgtatggg agggatcgtt
     4261 ctgagccatg gcacatcgat cagcaggaac gttgtcactt ccgtggccga cggccaacca
     4321 gcgccccgcc aagcagcgag tggcgatgga ccacaccctg ctggtggtga ccgtcacgtt
     4381 ggccttggtc ggcctggtca tggtgttcag cgcgagtgcg atcgttgcgg ggaatcgatt
     4441 tcacgattcg ggcttctttc tcaagcggca ggtagcatgg ctcgcgtttg gatttctgct
     4501 tctgcacctc acctcacgaa tcgattacac gctgtggaaa aaactgtcga ttccgatcct
     4561 cggttgcatg gtggctctgt tggtcatggt gttggttccg tcgctgggcg tggcagcgaa
     4621 gggcgcccgc cgttggctgc gattgggtcc gatgtcggtt caaccggccg aactggcaaa
     4681 gctggtctcg gtgatctatc tcgcggccta cctgacgaag aaagagggga agatcacgct
     4741 ctttcaaggc gggttactgc ccgcgttgac gatcatcggc ctgctgagcg gattgatcct
     4801 tctcgaaccc gatctgggta ccgtcgtcgt gatcggactc gtcacagtgg gcctgtgctt
     4861 tttggccggc gcgaaactct cgcatctgct cggattggcg ctctgtgcca tcccagcggt
     4921 gttagttctt gtcctgggat ccggttatcg gcgccaacga ctcttgacgt tcctggctcc
     4981 gtggaaggac gcctcggatg ccgggtttca gatcacccag tcgttcttag ccttcgggag
     5041 cggaggtccg ttcggtgtgg ggttgggaga aggcaaacaa aaactcttct tcttgccgga
     5101 agcccacacc gattttgtct tggcccttat cggcgaagag ctgggcctgg ccgggacggc
     5161 aactgtcatt ctgttgtttg ccgtttttgt gtggcgtgga tttcagattg cggcgagggc
     5221 gcgaatgccg ttcggccggt atctgggcat ggggatcacc ctcttgatcg gcatacaagc
     5281 gctcgtcaat gccgcggtgg tgaccgggct gttaccgaca aaaggattga cccttccatt
     5341 cgtcagctat ggtggatcat cggtggtagt cagtcttctc ggagtggggg tcctgctcag
     5401 tatctcccgc gatcggcacg ggggcggaca acgaggcgaa cgtggtgagt cggcacaccg
     5461 atgacgatcg ttattgcagc gggaggaacc ggcggccatc tctatccggc ggtcgcgctg
     5521 gctcgggcgt ttcagcggaa ggatgctgcc accagcatca tttttgtcgg tacggcacgt
     5581 ggattggagt cgaaagtgct cgcgcacgaa ggattcgaac tggtgttgat cagcgcgaag
     5641 ccggtgatgg ggaagggcct aatcgaggct gcacgaggtc tgctctcgct gccgatcagc
     5701 ttgtggcagt gtctaaaaat actgaaggtc cggcgcgccg atctcgtcat tggggtcggc
     5761 ggatatacca gtcctatgat ggtgctggct gcgtcgctca tcggcatcgc gcgggtgatc
     5821 ctcgaaccca acgcctatcc cggcatggcc aacaaggccg tcgggtcgtt tgcccagcgg
     5881 gtgtttcttt cattcgaatc tgcggcggca gcgttcgatc gacgaaagat tcgcgtcgtg
     5941 ggcacaccga ttcgcaagga atttgtcgag cagtcgttgc agaaagaagc tgctactccg
     6001 gcgcccggcc agcaccgggt gctgatcttc ggaggaagtc aaggggcgcg agcgatcaac
     6061 agtgcggtcg tcgaagggtt gcctgatttg atgaaacgcc atcctcagct gtcactcatt
     6121 caccaaaccg gcgacgccga tttcggtcgc gtcgccgaag cctaccgtcg cgcggggatc
     6181 tctgcgcagg tgactccgtt cctgtatgac atgccgtcgg tgttgcgatc ggctgatctg
     6241 gtcgtggcga gagccggtgc catgactatc gcagagctga ccgcatgcgg gaagcccgcc
     6301 attctgatcc cgctgcccca ggcgatctac aaccatcaga cgcacaatgc gaaggttatg
     6361 gaggctgccg gcgctgctgt cgttctacct caagctgaat tgactggccc ccggctgacg
     6421 gacgcgatca caagaattct cgatgatcca gagcggctgc gaacaatgag cgatcgcagt
     6481 cggaacatga ggcgaatcga cgcggccgac tcgatcgtcc gcgaatgcta cgcactcaca
     6541 ggagaccacc atgatggcca ccagtctgtt ggagcggcgg gcgtctaatg ggatcgggca
     6601 acgcgagacc gagtcacatt catcgctgct gttgggaggg gtaaagcgag tgtcgatgtt
     6661 tcgcaagacg caacagattc acttggtcgg gatcggcgga gccgggatga gcggaattgc
     6721 cgaggtgttg ctgaccctcg gctacaaagt cacaggttcc gatcttcagg catccgatac
     6781 aaccaggcgg atcgaagaac tcggtggccg ggtcttcatc ggtcatcagg aatcgaacgt
     6841 gggcgacgct caggtcgtcg tgatctcatc ggcggtgtcg gcggagaatc cggaagtcat
     6901 ggttgccaag gccaaacaga ttccagtcat cccgcgggcc gagatgttgg cggaactcat
     6961 gcggctcaag tttggtgtgg ccattgcggg cgcacatggc aaaacaacga caacgtcgat
     7021 ggccgccaat gtactcgctc aaggcggctt ggacccgacc atggtcatcg gcggaaaagt
     7081 caatgcgctc ggcagtcatg cccggctcgg tcgtggcgat gtactggtcg cggaagccga
     7141 tgagagcgac ggctcgtttc ttcggctgtc gcccacgatc gtcgcggtga ccaatctcga
     7201 tcgcgagcac ctcgaccact atgggagcat ggagcggatc aacgagagtt ttctcgagtt
     7261 catcaacaag gttcccttct atggactggc cgtgctctgc tcggacgatg aacggctccg
     7321 cgcacttttc ccgcgcatcg tgaagcgcta ccacacctac ggactccagg agcgcgaagg
     7381 cggagggcct gatttcaagg cgaccgacat tagcttgaag caatggggcg ccgagttccg
     7441 cgcccacttc cgagggcgca acctcggtcc gtttcgtctg tctgttccgg gaatccacaa
     7501 cgtctcgaat gctcttgcag ccatcgcgat cggcgtggaa ttagacgtgc cggcggatct
     7561 gatccgcaag gccttggctg cctttactgg agtggagcga cggttccatc ttcgcggaga
     7621 ggcgaacggg atcatggtgg tagatgacta cggtcaccat ccgacagaaa tcaaagccac
     7681 gttggctgca gcgaaacagg ggtgggagga tcgccggctg gtcgtcctct tccagccgca
     7741 ccggtattca cgttcacgag attgtgccga ggaatttgcc catgccttcg atcaggcgga
     7801 cctgctcttt atgaccgaaa tttatgccgc gggagaacaa cccatccccg gcgtttcggg
     7861 agccaagctg gctgaatcgg tccgagctgc cggtcatcag ggcgtgacgt tcgtgacgca
     7921 gaaagaaacc ttgcccgatc acgtgttgcc gcaattaaaa ccgggagacc tcgtgatcac
     7981 attgggggcg ggagacatat ggaaggcggg cacaggattg ctggcacggc tggctccttc
     8041 gtcatgatgc aatgaacgtc gagggtcgtc gtcatggatc aaggtcccgt gcaccgacat
     8101 tcgagaagaa ggatctgcac gcggccgttg tcggtctcaa aggcactgtg tcctttcatg
     8161 cgtcgctgcg cgactacacg tcgttcaaaa tcggcggccc ggcggacgtc ttggtcgagc
     8221 cggcggatgt cgacgatgtc tgtcgcctgg ttcgc
//
LOCUS       DATDNW010000061         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1030346, whole genome shotgun sequence.
ACCESSION   DATDNW010000061 DATDNW010000000
VERSION     DATDNW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030346"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /gene="hemL"
                     /locus_tag="VI359_01625"
     CDS             <1..1214
                     /gene="hemL"
                     /locus_tag="VI359_01625"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY6972980.1"
                     /translation="VRAFRSVGGQPRFIQRAKGARLYDVDGNSYLDYVLSWGPMILGH
                     APTTVITAIKKAAANGTSYGAPTELEVTLARMIREALPSMEKVRLVSSGTEAVMSAIR
                     VARAFTKRDAILKFEGCYHGHSDYLLAKAGSGLATLGLPDSPGVPADFAKHTLTAPYN
                     DIQTVRRIVNEYRDMLACIILEPIAGNMGVVPPAAGFLQALRQLTTENGILLIFDEVI
                     SGFRVTYGGAQTLYGVTPDLTVLGKIIGGGLPVGVYGGREEIMDLIAPSGPVYQAGTL
                     SGNPLAVSAGIATLKQLKVRGIYKKLEQRSAALAKGMGEAAKKTRVPFTQTRVGSMLC
                     GFFTPGPVVDWNSAKQSDTKRYGIFFHMMLEQGVYFAPSQFEAAFLSTAHTSSDIEKT
                     IRAAHMAFKSL"
     gene            complement(1349..1579)
                     /locus_tag="VI359_01630"
     CDS             complement(1349..1579)
                     /locus_tag="VI359_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972981.1"
                     /translation="MDMMGRVGLVEIPVLIAPDQKVWMDRMIKEGKIAIPPGGTLEKG
                     SLYSMFIRMLLHNAMEEQKRQEALEAADEDDE"
     gene            1746..>2033
                     /locus_tag="VI359_01635"
     CDS             1746..>2033
                     /locus_tag="VI359_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972982.1"
                     /translation="MRLLGIRMGRALRTPACARRSRQGYLSVVVWSAILTLITPHILP
                     AQQSTAPPSSLPATPLSPSDNVSGQLPSCDLCRKPESRAGSDVRPHRLPRDK"
BASE COUNT          416 a          598 c          563 g          456 t
ORIGIN      
        1 cggttcgagc gtttcggtct gtcggtggac aaccgcgttt catccaacga gccaagggtg
       61 cgcgcctcta cgacgtcgac ggaaacagct acctcgatta cgtcctgtca tggggaccga
      121 tgatcctggg ccatgccccg actacggtga tcaccgcgat caagaaagct gctgccaacg
      181 gcaccagtta cggcgcaccc accgagttgg aagtcacgct cgcgagaatg atccgtgagg
      241 cgcttccttc gatggagaag gtgcggctag tcagctcagg cactgaggcc gtgatgagcg
      301 cgatccgcgt cgcacgtgcg ttcaccaagc gcgacgcgat cctgaagttt gaaggttgct
      361 accatggtca cagcgactat ctgctggcga aggcaggatc agggctcgcg acgttgggcc
      421 ttcccgattc ccccggcgta ccggccgact ttgcgaagca taccctcacg gctccataca
      481 acgatattca gacagttcgc cggatcgtca acgagtatcg cgacatgctg gcctgcatta
      541 ttctggaacc gattgcgggg aacatggggg tcgtgccgcc ggctgcaggt tttctccagg
      601 ccctccgtca gctcacgacc gaaaacggaa tcctgttgat cttcgacgaa gtgatttccg
      661 gtttccgcgt cacctatgga ggcgcacaga cattgtacgg tgtcacgccg gatctgacgg
      721 tgctggggaa aatcatcggc ggtggcctcc cagtcggcgt gtacggggga cgagaggaaa
      781 tcatggatct gattgccccg tccgggccgg tgtatcaggc cggtaccttg tcgggcaatc
      841 ccttggctgt atcggccggc attgccactc taaagcaact caaagtgcgc gggatttaca
      901 agaagctgga acaacgatca gcggctctgg ccaaagggat gggcgaagca gccaagaaaa
      961 cccgcgtgcc gtttacgcaa acgcgagtcg gttcgatgct ctgtggattc tttacgcccg
     1021 ggccagtggt ggattggaac agcgcaaagc agtcagatac gaaacgctac ggcatcttct
     1081 ttcatatgat gctggagcag ggcgtctact ttgctccatc gcagttcgaa gctgcgttcc
     1141 tctcgaccgc tcatacctca tccgacatcg agaagaccat ccgagccgca catatggcat
     1201 tcaaatcgct ctaagagctg atcgcccgtc gttcgtatca cgtatctcgc tggatacaga
     1261 acgcatctgt tcagatacac ggggcggtcg gatttgctga cgtgtcctgc ctgtttagac
     1321 aggaagcttc cattatgctt cccgagtcct actcatcgtc ctcgtccgct gcctccaacg
     1381 cctcctgccg cttctgttct tccatcgcgt tgtgcagcaa catgcggata aacattgaat
     1441 agagcgagcc tttttccaag gtgccgcccg gcggaatggc gatctttcct tctttgatca
     1501 tgcgatccat ccagaccttc tgatccggtg cgatcaacac cgggatctcg accaatccta
     1561 cacgtcccat catatccatg agaagcctcc gtgagtcgta gttcgtgaaa tgtatcttgt
     1621 tgatgatgca gagacagatg gtatttttgt cgacagaatg aacgaccttt tacgagcaac
     1681 gaggtacgcc gctataacag catgcggctt tttgcattgt caaccgcgcc ttcgtggcac
     1741 ggagcatgcg tttgcttggg atccgtatgg gacgcgcctt acgaacaccg gcttgtgcga
     1801 ggagatcccg gcaaggctac ctgagcgtcg tcgtctggtc ggcgatactc acgttgatca
     1861 ctcctcacat cttaccggct cagcaatcta ccgctcctcc atcatcgttg cctgccacgc
     1921 cgttgtcgcc cagtgataac gtctcgggtc agctgccatc ctgtgatctc tgccggaaac
     1981 cggagtcgcg ggcgggaagc gacgtccgtc cccatcgact tcctcgtgac aag
//
LOCUS       DATDNW010000062         9787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_484858, whole genome shotgun sequence.
ACCESSION   DATDNW010000062 DATDNW010000000
VERSION     DATDNW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484858"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1786
                     /locus_tag="VI359_01640"
     CDS             161..1786
                     /locus_tag="VI359_01640"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6972983.1"
                     /translation="MKGQNGLVVVAFIAIVTSPSFAQVTGDAPGVRPDAIVDLKSDDG
                     IALVKGQWRYSNTRVVEVDHRSPGMDLGPSGPPNRTQDINLHAGTSDFDDSKWETLKP
                     LQLEERRSTGRLCFNWYRTAITIPDKVGSFDPTGSTVVFEIAIDDYAEVWVDGKLPLV
                     LGQPGGQLIKGFNAPNRVVLTSNAQPGQRIQLAVFGINGPLSNPPGNFIWVRSATLDF
                     YKSRHVGPTQFVKTDIVKVDPSLDAIVSPDAKLEKLAGGFLFTEGPVWVPAKDSIPGY
                     LLFSDPNANTIYRWSADGQVSAFRTKSGYAGFNVGEYHQPGSNGLTLDKQGRLTINQH
                     GNRRVIRVEPRGNITVLADRYDSKRLNSPNDLVYRSDGALYFTDPPFGLPKVFDDPRK
                     ELPYSGVYCVKDGQVKLVSIDLDAPNGLALSPDEKTLYVNNWNDKRKIILRYDVNPDC
                     TLSNSTLFFDMTNAPGDDALDGLKVDQQGNVYSTGPGGLWIISPEGKQLGLIKGPEDP
                     HNMAWGDDDGKTLYITALTGIYRIRLNIPGVRP"
     gene            1783..2793
                     /locus_tag="VI359_01645"
     CDS             1783..2793
                     /locus_tag="VI359_01645"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY6972984.1"
                     /translation="MKHVSSSLWAFVTLIVLLYSEPASTEPVSATISRVDPRFDQLVP
                     KEAQLEKIADGFTWVEGPVWNKQGGFLLFSDIPANAVYKWKAGEGTSVYLRSSGYSSA
                     TPFEGKEPGSNGLTFDSAGRLVLCRHGDRQIGRLEADGHIATLADRYNGHRINSPNDL
                     VFKSNGDLYFTDPPFGLPKAFDDPGKAAVQGVYRLAKDGTVTLLINDIKAPNGIAFSP
                     DEKTLYVSDVDPKRAAWLAYDVKEDGTLTNGRLLFDATRWRKDPYFGPDGFKIDQQGN
                     IFGARPGGITVMASDGTLLGTIETGQPTSNLAWGEDGRTLFVTGGGAVYRLRLTTTGA
                     RY"
     gene            2907..3191
                     /locus_tag="VI359_01650"
     CDS             2907..3191
                     /locus_tag="VI359_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY6972985.1"
                     /translation="MLTEFVARITPPCTDITRLVSESMDRTLPVSTRIKLRLHLMICA
                     ACAEYRRQLQQIRRALHHSATKPEEQDPNAPVLSPTAMAQLKKALRAKTE"
     gene            3258..3740
                     /locus_tag="VI359_01655"
     CDS             3258..3740
                     /locus_tag="VI359_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972986.1"
                     /translation="MRVAMCLLTIILSGCQGIPILDRVDRDPPFSVWPLWEQYRQCLT
                     ATDPADLLSTVERLERAMRAGTEPPAWIALLGEHVARQPLRTAVDPYALGAACTLRTA
                     GVMLDAQRLAEARLLYQRVLARYEAREFAYYREQAKEALARLEAPLPAVVALRTNLSR
                     "
     gene            3854..4066
                     /locus_tag="VI359_01660"
     CDS             3854..4066
                     /locus_tag="VI359_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972987.1"
                     /translation="MNATIYWTTQRVQRKAQEFRWAAQLWWLNQQVEFVGWRYRLEAL
                     LPSTMPLAVSRRSNRTRRVQAHAVMG"
     gene            4050..4871
                     /locus_tag="VI359_01665"
     CDS             4050..4871
                     /locus_tag="VI359_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY6972988.1"
                     /translation="MQSWADSLRRHWPEYLMEAAGLGIFMVSAAVVTTLFEYPKSPLH
                     ELLPDPVVRRVLIGIAMGLTAIGIIYSPWGQQSGAHLNPAVTLTFFRLKKIEGIDALS
                     YVIAQSVGGVIGLVLASVAIGMAIEHPAVNYVVTVPGNNGPGIAFLAEALISFGLMLT
                     VLLVSNHQSLAPVTGMFAGCLVATYISLEAPFSGMSMNPARTLASALPADIWTSFWVY
                     LAAPPLGMLLAAELYTHMKGLKQVYCAKLYHHNNKRCIFRCNYAALAASDDSPPS"
     gene            5009..5587
                     /locus_tag="VI359_01670"
     CDS             5009..5587
                     /locus_tag="VI359_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6972989.1"
                     /translation="MTTRILATLVLVTAFAAPVWAGFDEGMAAYERGDYSTTLKEWRP
                     LAEQGDPTAEHHLGWLYVIGRGVPQDYEEGVRWLRKAAEQGDSDAQTNLGSLYLLGEG
                     LPQDYTEALKWLRAAADQGHPFAQTKLGIMYEDAHGVPQDRVQAHMWFSLAAAQGSEL
                     AGAFRSDLTKHMTLVQIDEAQRLARGWKPKGK"
     gene            complement(5960..7579)
                     /locus_tag="VI359_01675"
     CDS             complement(5960..7579)
                     /locus_tag="VI359_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6972990.1"
                     /translation="MMAECTQTRQEVLRIFLARARRYGMLLLALSLSACSDWLPHVDL
                     APSYEPPQYVVPASWHGETPFVEAKPSDGELRQDWWKLYDDALLNSLIEQAMAANPDL
                     QAAAERFVQARDVMMQVRSQRIPQVGLGGKAADSRNHIDPLRIPGDLALTGPVGLGAG
                     LASWEPDFWSAIRNRTLVETYRAEERAADWGLARLSLQAEIATDYFTLRGYDAQSAIY
                     KQSIDLYRNSLKLVKAQFAGAIASALDVARVESLLFGTETKYAQIQGQRQVTEQAIAI
                     LVNMAPASFKIDPVDDLRMTKFTIPRSIPSTLLERRPDVAAMERRMAEASRAIGIARA
                     AFYPDVRFSADGGIIDTGFDIAKLTAAMWSYGASISLPVFQGGYRRAQLQRSWSAYRE
                     TEDLYRSTVLNAFREVENGLSLTNQLTIAANRQDAAVGANFKAQNLTMELYQGGLASS
                     LELIYAQVQTLSARIDLVLIKADLLKSTVALLRALGGGFNRKQLPTDEQIQPFGTFQY
                     VNLDKPSPAGGIDVNAGNNWVNNDLTKPAVP"
     gene            complement(7593..8870)
                     /locus_tag="VI359_01680"
     CDS             complement(7593..8870)
                     /locus_tag="VI359_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6972991.1"
                     /translation="MMKTLSGKQIALLACLLCVLYLGYRIYGSKSDAALLSEKTLEGA
                     VPTVAVVNPKPGPPTETITLPGNVQAWFQAPIYAQVSGYVKMWYKDYGALVKKGDILA
                     EINAPALDAQYAQAKADLESERAKYALADITAKRWVALRKNGAVSEQSISVKVAEAKA
                     EAARVNAAEQNVRNYEALIRFKTIVAPYDGVVTQRNINVGDYVNKEGTVSHTNALSNL
                     FTVADVSLLRLFVSVPAAFGAFLQPGLTADVTVPQLPHRHFTGKFLTVARGFDVSTRT
                     AITVFTIDNEDRALWPGSYAKVTLTAPVDRNVLTIPSTALVFQEHGTQVAVVTEDDRV
                     HLKPITVVKLMDNVVEVAGEGISTSDRVVNNPSASLLEGEKVRVVTPAPGYDLMSGDT
                     PESKETPASTEDQGSKEIHGSTEAPAPKEHSIK"
     gene            complement(8867..>9787)
                     /locus_tag="VI359_01685"
     CDS             complement(8867..>9787)
                     /locus_tag="VI359_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6972992.1"
                     /translation="KDMAYNLLMTTAGSQQVDQVYWLDPSTGMSYLINVYTPQSQINS
                     VNSIKTVPVNSSDDSSDDSVQLLGNLASLSPEGTPGVITHGNIMPLLDIYVSAEGRDL
                     GGVLADVLKINDAMKTELPSSAAVEIHGQAALMYEAYAELIFGLLAAIVLVYLLIVVN
                     FQSWLDPFIIITALPGALAGIAWALFLTHTRLSEPALTGAIMTMGTGTANSILVVSYA
                     RERFQEHGDALRAAIEAGKTRFRPVLMTASAMILGMVPMATGYSQNAPLGRAVIGGLL
                     VATVFTLFLVPCVYAIMYSRRTARQKEEAS"
BASE COUNT         2070 a         2901 c         2848 g         1968 t
ORIGIN      
        1 gtaaaggtgc ggtaagaaga ggcgtacgag ggcctcgcct cttcgctcgc agaacgcgca
       61 cgataagaat gtgctcgttc gacgcgcgcg gttgaggcca aggccctcgt ctccccgcac
      121 agggctgaac caaatggcag agggaaagag agaagaaaag atgaagggtc agaatgggct
      181 cgttgtcgtt gcgttcatcg ctatagtcac ttctccatcc ttcgcgcagg tgacggggga
      241 tgcgccggga gtacggccgg acgcgatcgt cgacttgaag tcagacgatg gcatcgcact
      301 cgtcaagggt cagtggcgtt acagcaacac cagagtggtt gaggtcgatc atcgcagccc
      361 aggtatggac ctcggccctt ccggtccgcc caaccgaacc caggacatta atctccacgc
      421 aggcacatcg gacttcgatg actcaaagtg ggagacgttg aagccattac agttggagga
      481 gcgtcggtcg accgggcgcc tctgttttaa ttggtaccgg accgccatta cgattcccga
      541 caaggtcggc tcgttcgacc caaccggctc tacggtcgtt ttcgaaatag ccatcgacga
      601 ctatgccgaa gtatgggtcg acgggaagct gccgctcgta ttgggacaac cgggcggaca
      661 gctcattaaa ggatttaacg ccccgaatcg cgtcgtgctg accagcaacg cccaaccggg
      721 ccagcggatt cagctggccg tgtttggaat caacggcccg ctctctaatc cgcccggcaa
      781 cttcatttgg gtccgctccg caacactcga tttctacaag agccgtcacg tcgggccgac
      841 gcaattcgtc aagaccgaca tcgtgaaggt tgacccatca ctcgatgcga ttgtatctcc
      901 agacgccaaa ctcgaaaaac tcgccggtgg ctttctcttc accgagggac ctgtgtgggt
      961 cccggccaag gattccatac ccggctattt gctcttcagc gatcccaacg ccaacacaat
     1021 ctaccgttgg tcagctgacg ggcaagtctc ggcctttcgc acgaaaagcg gctatgccgg
     1081 gttcaacgtc ggtgagtacc atcagccggg atcgaacggg ctgacgctgg ataaacaggg
     1141 ccgcctgacc atcaaccagc acggcaatcg ccgagtcatt cgcgtcgagc cgcgcggcaa
     1201 cattaccgtg ttggccgatc gctacgacag caagcgactc aatagcccga acgatctcgt
     1261 ctatcgctca gacggtgcgc tctacttcac cgatccgccg tttggcttac ccaaagtttt
     1321 cgacgacccg aggaaagagc tgccctatag cggtgtctat tgcgtgaagg atggacaagt
     1381 gaaactcgtc agcattgatc tcgatgcgcc gaacggtctc gcgctctcac cggacgaaaa
     1441 gacgctctac gtgaacaact ggaacgacaa gaggaagatc atccttcggt acgacgtcaa
     1501 tccagattgc accctgtcga acagcacgct cttcttcgac atgacgaatg cgccaggcga
     1561 cgatgcgctc gatggtctca aagtcgatca gcaggggaac gtctattcaa ccggacctgg
     1621 cggactttgg attatctcac cggagggaaa gcaactcggt ctgatcaaag gtcctgaaga
     1681 tcctcacaat atggcttggg gcgacgacga cgggaagacg ctctacatca cagcactcac
     1741 gggcatctac cggatcagat tgaatatccc gggggtacgg ccatgaaaca cgtatcatca
     1801 agcctatggg ctttcgtcac gttgatcgtg cttctgtaca gtgaacccgc atcgactgaa
     1861 ccagtctcgg ccaccatctc ccgagtggat cctcgcttcg atcagctcgt gccgaaggag
     1921 gcacagctcg aaaagatcgc cgatggattc acctgggtcg aaggaccggt ctggaacaag
     1981 caaggaggct ttctgctctt ttccgacatt ccagccaatg cagtctataa atggaaggct
     2041 ggcgaaggga cgagcgtcta tctaagatca agcggctaca gtagcgccac accatttgaa
     2101 ggtaaggaac cgggctcgaa cggcctgact tttgactccg cgggacggct tgtcttgtgt
     2161 cgccacgggg atcgtcagat cggacggctc gaagcagacg gtcacattgc cacgctcgcc
     2221 gaccgataca acggccatcg aatcaacagt ccgaacgatc tggtattcaa atccaacggg
     2281 gatctctatt tcaccgaccc tccttttgga ttgccgaaag cattcgacga tcccgggaaa
     2341 gctgcggtcc agggtgtcta ccggctcgca aaagacggca ccgtcaccct actcatcaac
     2401 gacatcaagg caccgaacgg aattgcgttc tcgcctgacg aaaagacgct ctacgtctcc
     2461 gatgtcgatc cgaagcgagc cgcctggcta gcgtatgacg tgaaagaaga cggcaccctc
     2521 acgaacggtc gcctgttgtt cgatgccacc cggtggcgga aagatcccta cttcggacct
     2581 gacggattca aaatagatca acaaggaaac atcttcgggg cgcgaccagg cggcattacg
     2641 gtcatggctt cggatgggac cttgctaggt acgatcgaaa ccggccagcc cacttccaac
     2701 ctcgcctggg gcgaagacgg ccggacgctg ttcgtgacgg gcggcggggc cgtgtaccga
     2761 ttgcgcctga ccaccacagg agcccggtat tgagggctct gtaagaaatc gccaccttga
     2821 tcgactaccc cgtgcagacc ccgttgcagc tggctatgat gggacagccg caagaatgat
     2881 attctactac ctcgcgtaac ggacggatgc tgaccgaatt tgtagctcgt atcacccctc
     2941 cctgcacgga catcacccgg ttggtgtccg agtccatgga tcggacgctt ccagtctcga
     3001 cccgtatcaa gctccgtctc cacctgatga tctgcgccgc ctgcgcagag tatcgccggc
     3061 aactgcagca gattcgccgg gcattgcatc actccgccac aaagccggag gaacaggatc
     3121 cgaacgctcc agtcctctct ccaactgcca tggcgcaact gaaaaaagcc cttcgagcca
     3181 agaccgaata ggtcagtaca ccctcgccag tttgctcaat tcttgtaaga gagacagccg
     3241 gtccacgact gatggccatg cgtgtcgcca tgtgcctgct caccataatc ctgagtggat
     3301 gccaaggaat accgattctc gatcgggtgg acagagaccc gccgttctcc gtctggcccc
     3361 tgtgggaaca atatcgccag tgtctgaccg ccaccgatcc cgccgacctg ctctcgacgg
     3421 tggagcgatt ggaacgcgcg atgcgggccg ggaccgaacc gcccgcctgg atcgccctgt
     3481 tgggagagca tgtcgcacgg caaccgttgc gaacagccgt cgatccgtac gcgctcggcg
     3541 cggcctgtac cctgcggacc gccggcgtca tgctcgacgc acagcgtctg gcggaagccc
     3601 gcttgctcta tcaacgagtc ctcgcccgct atgaagcccg tgagttcgcc tactaccgcg
     3661 agcaggcgaa ggaggccttg gcgcgtcttg aggcaccctt gcccgctgtc gtcgcgctcc
     3721 gcaccaacct gtcccgttga gatcgatcgc cctacccgca ccgcgaactt cgtgattctc
     3781 gttcagtgcc tcacggcatg ctgtaagaag cgatgcctcg gggcgactga tcaacagaaa
     3841 agcgaggtga tcaatgaatg ccacgatcta ttggacgaca caacgagtgc aacggaaggc
     3901 acaggagttc aggtgggcgg cccaactctg gtggctcaat caacaagtcg agtttgtcgg
     3961 atggcggtac cgtttagagg cgctcctgcc atcgacgatg ccgctggcgg tcagcaggcg
     4021 atcgaacaga acgagaagag tccaggccca tgcagtcatg ggctgacagc cttcgccgac
     4081 actggcctga gtacctgatg gaagcagccg gcctcgggat ttttatggtc tctgcggccg
     4141 ttgtcacgac ccttttcgag tatccaaaat ctccactgca tgaacttctg ccggatccgg
     4201 tggtgcgacg cgtgctgatc ggcatcgcga tgggactcac cgccattggc atcatctact
     4261 cgccctgggg ccaacaatca ggcgcgcatc tcaatccagc agtcacactc acgtttttcc
     4321 ggttaaagaa aattgagggg atcgacgcgc tgtcctacgt gatcgcgcaa tcggtgggag
     4381 gggtcatcgg actggttctc gcctctgtcg ccatcggcat ggcgatcgaa catcctgcgg
     4441 tcaattatgt cgtgacagta ccgggcaaca acggtcctgg gattgccttt ctcgctgaag
     4501 cgctcatttc attcgggctc atgctgaccg tgctgctggt ctcaaaccat caatcgctcg
     4561 ctccagtgac tgggatgttc gccggctgcc tcgtcgccac ctatatcagc ctcgaagcgc
     4621 cgttctccgg tatgagcatg aaccccgcac gcaccttggc ctcagcctta ccggcggaca
     4681 tctggacttc gttctgggtc tatcttgcgg cacctcccct cggcatgctc ctcgcggctg
     4741 aactgtacac gcacatgaaa ggtctcaaac aggtctactg cgccaagctg tatcaccaca
     4801 acaacaaacg ctgtatcttt cggtgcaatt atgcggcgct cgcggcaagt gacgacagcc
     4861 cgcccagctg actgctgcct gagaatgaga ctttcaggcc cccgctttga actcaacccg
     4921 tcacaagtaa ataagaaatc tcctcacaga ggggttgtca ccttttcgcg gtgggcgtat
     4981 cgtccagcca ataatgagga gatgatccat gacgacacgc attctagcca ccctggtcct
     5041 ggtaaccgcg ttcgccgcgc ctgtgtgggc agggtttgat gaagggatgg cggcgtacga
     5101 acgtggcgac tattccacca ccctcaaaga gtggcggccg ctggcagagc agggagatcc
     5161 cacggccgag caccacctgg ggtggctata cgtcatcggt cgtggggtgc ctcaagatta
     5221 cgaagaagga gtccgctggt tgcgcaaggc cgcagagcaa ggagatagtg acgcccagac
     5281 caatctagga agtctctatc tcttgggaga agggcttcca caggattaca ccgaagccct
     5341 gaaatggctc cgcgctgccg ctgaccaggg tcatccgttt gcgcaaacga agcttggaat
     5401 catgtatgag gacgcacatg gggtgcctca agatcgcgtc caggctcaca tgtggttcag
     5461 cctggcggcg gcacaaggat ctgaattggc aggcgcattt cggagcgact taaccaagca
     5521 catgactctc gttcagatcg atgaagcgca gcggctcgca cgagggtgga agccaaaggg
     5581 gaagtaagtg gtgtgtcgat cgaggatggt gggggatgag gaagaatgaa agaatgaggc
     5641 tgaggcctgg cccaggagaa ggagaaacca cggactggtc agcaggggcc actaccgcca
     5701 gaatctccaa gcgatccaac cgtcgttggg tgcctttctc cgctttgaaa ttcaacggat
     5761 tactctggca gcttagacag gcgattcata tggttttgtc agaaggacac cagagccgga
     5821 cgtggagacc gatagaaggc ggtcaagccg cgcgagactc gatacggcta ggcggctcgc
     5881 ccctaccctt cgggaatttc actgggcgga agcgtcaagc ttcgattgac gcactaacat
     5941 acatgtttgt gaacgacggt caaggcacgg caggtttggt cagatcatta ttgacccagt
     6001 tattgcctgc gttgacatcg attccccctg caggcgacgg cttgtcgaga ttgacatatt
     6061 ggaacgtccc gaacggctgg atctgctcgt ccgtgggcaa ctgcttgcgg ttaaagccac
     6121 cgccgagcgc gcggaggagt gccaccgtgg atttcagtag gtcggccttg atcagcacga
     6181 ggtcgatgcg tgccgagagc gtttggacct gcgcatagat gagctcaagg ctggatgcga
     6241 gcccgccctg atagagctcc atggtcagat tttgcgcctt gaagttcgcc ccgacggcgg
     6301 cgtcttgccg attagccgcg atagtcagct ggttcgtcaa gctcagcccg ttctcaacct
     6361 cccgaaacgc gttgagcacc gtcgaccggt acaagtcttc cgtttcgcga taggccgacc
     6421 aggaccgctg caattgggca cggcgatagc cgccctggaa aaccggcagc gagatgctgg
     6481 cgccatagga ccacattgcg gcggtcagct tggcgatatc gaagccagtg tctatgattc
     6541 ctccgtcggc tgagaaccgt acatcagggt aaaaggcggc gcgtgcgatc ccgatggcac
     6601 ggctggcctc cgccattcgg cgctccatcg cggcgacgtc tggccgccgc tccaacagcg
     6661 tggagggaat gcttcgcgga atcgtgaatt tggtcatccg caggtcatcg accgggtcga
     6721 tcttgaaact cgccggagcc atgttgacta gaatggcgat ggcctgctcc gtcacctggc
     6781 gctggccttg gatctgcgcg tattttgtct ccgtgccgaa cagcaacgat tcgacgcggg
     6841 cgacatcgag tgccgacgcg attgcgccgg cgaactgagc cttcacaagc ttcagcgaat
     6901 tcctatagag gtcgatcgat tgtttgtaga tcgcgctctg ggcgtcgtaa ccgcgcagcg
     6961 tgaaatagtc ggtcgcaatt tccgcctgta ggctgaggcg agcgaggccc cagtcggccg
     7021 ctcgctcttc ggcgcggtag gtctccacaa gcgtcctgtt gcgaatcgcc gaccagaagt
     7081 cgggctccca ggaggcaagc cccgcaccga gcccgaccgg cccggtgagc gcaagatcgc
     7141 cgggaattct caacggatcg atatgatttc gactgtcagc ggctttcccg cccaagccga
     7201 cttgcgggat ccgttgggag cggacctgca tcatgacgtc gcgagcctgg acgaagcgct
     7261 cggcagccgc ctgcagatcc ggattggccg ccatggcctg ttcgataaga ctgttcagca
     7321 gcgcgtcgtc gtacagcttc caccaatcct ggcgcaattc gccgtccgac ggcttcgcct
     7381 cgacaaaggg agtctctcca tgccacgagg cgggaacaac atactggggc ggttcataag
     7441 agggcgccag gtcgacgtgc gggagccaat cgctgcaagc cgacagactg agtgccaaca
     7501 gtaacatgcc atagcgccgc gcacgggcca gaaagatgcg caatacttct tgtctcgttt
     7561 gggtgcactc agccatcatt ttcaagcgaa tgtcatttta tagagtgttc ctttggcgcg
     7621 ggagcttccg ttgacccatg aatttccttt gacccttgat cttccgttga cgcaggcgtc
     7681 tcctttgact ccggcgtgtc tcctgacatg aggtcataac caggtgctgg cgtcacgacg
     7741 cgcaccttct cgccttcgag caacgaggcg ctcgggttgt tcacgacgcg gtcgctcgtg
     7801 gaaatcccct cgcctgccac ttctacgacg ttgtccatga gtttgaccac ggtaattggt
     7861 ttcaagtgta ctcggtcgtc ctccgtcacc acggccactt gcgtgccgtg ttcctggaac
     7921 accaaggcag tggacggaat cgtgagcaca ttccggtcaa ccggcgcagt gagcgtgacc
     7981 ttggcgtagg acccgggcca gagtgctcga tcctcgttgt cgatcgtgaa gacggtgatc
     8041 gcggtgcgcg tgctcacatc gaatccacgg gcgacggtca ggaatttgcc ggtgaaatga
     8101 cgatgaggca attgcggcac cgttacgtcg gccgtcaggc ccggctggag gaaggccccg
     8161 aaagccgccg ggacggagac aaagaggcgc agtagactga cgtcggccac agtaaagagg
     8221 ttgctgaggg cattggtgtg gctgaccgtc ccttccttat tgacgtaatc accgacgttg
     8281 atgttgcgct gagtcactac gccgtcatag ggcgcgacga ttgttttgaa ccgaatgagc
     8341 gcctcgtagt tcctgacgtt ctgctccgcg gcattgaccc tcgccgcttc ggctttcgcc
     8401 tctgcgactt tcaccgagat cgactgctcg gacaccgcac cgttcttgcg cagcgccacc
     8461 cagcgcttcg cggtgatgtc ggcgagggca tatttggcgc gctccgactc gagatccgct
     8521 ttggcttgcg catattgggc atcgagagcc ggggcgttga tttcggcaag gatgtcgccc
     8581 ttcttcacca gcgcaccgta gtccttgtac cacatcttca catagcccga gacttgcgca
     8641 tagatcggcg cctgaaacca agcctgaaca ttgccgggga gcgtaatggt ctcggtcggc
     8701 ggccctggct ttggattgac gacggccacg gtggggactg cgccttcgag cgtcttctcg
     8761 ctcagcagag cggcatcgct tttgctccca taaatccgat agccgagata caacacacac
     8821 agcaggcagg ctaaaagggc aatctgtttt ccactgagtg tcttcatcat gaggcctctt
     8881 ccttttgccg agcagttcgc cgactgtaca ttatggcgta cacgcagggc acgagaaaca
     8941 gcgtgaagac ggtggcaacg agcaagccgc cgatgacagc gcggcccagc ggcgcattct
     9001 gggaatagcc ggtcgccatg gggaccatcc cgaggatcat ggccgaggcg gtcatgagca
     9061 ccggacggaa gcgggtcttc ccggcttcga tcgcggcccg cagcgcgtcg ccgtgctctt
     9121 ggaaccgctc gcgcgcatag gacacgacga ggatcgaatt ggcagtcccc gtgcccatgg
     9181 tcatgatggc gcccgtcagc gcgggctccg acagacgcgt atgggtcagg aacaacgccc
     9241 acgcgatgcc tgccagcgcg ccgggcaagg ccgtgatgat gataaaggga tcgagccagg
     9301 attggaagtt gactacgatc agcagataga ccagcacgat tgcggcgagc agcccgaaga
     9361 tcagctcggc ataggcctcg tacatgagtg cggcctgacc atgaatttcc accgcggcgc
     9421 tgctgggcaa ttctgtcttc attgcgtcgt taatcttcag gacgtctgcc aacacccctc
     9481 cgaggtcacg cccttcggct gagacgtaga tgtccaacag cggcatgatg ttcccgtgcg
     9541 tgatcacgcc cggcgttccc tccggtgaca ggctggccaa attgccgagc aactgtacac
     9601 tatcatccga actatcgtcc gaggaattaa ccgggacggt tttgatacta ttgacgctat
     9661 tgatctgcga ctgcggcgta tagacattga tcaggtacga catcccggta ctggggtcga
     9721 gccaatagac ctggtcgacc tgttggctcc cggcggtcgt catgagcaga ttgtaggcca
     9781 tgtcctt
//
LOCUS       DATDNW010000063         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_210844, whole genome shotgun sequence.
ACCESSION   DATDNW010000063 DATDNW010000000
VERSION     DATDNW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210844"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VI359_01690"
     CDS             <1..907
                     /locus_tag="VI359_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972993.1"
                     /translation="GSVMILEGIVASKQERRFHLDMPPPRRYDGRTCWREALLLRPSP
                     SINSLPIFLLVFVVCAWSAPFSLIAAPYFEDGFLGLTQQELHEKLGKPQAVRDRKSAL
                     RVFTYYSMADWEKYFRKMVSPENGEDVYTFKREGIDIRYSFSYAVDPSDQNDNRTLFV
                     RLVDIEFAPPVPLGQVPSLVPEFKPSEDPSTPAFRSNIWVLLFKGTPSAEARFIVKEK
                     GKDSLSWSLAYQLFSLQGLPDPLTTKAPVDRIEISTQSVDLVRQRQRLTHDPILNPYS
                     KEFAQQQLVPQPPKTKKIPVPSYAD"
     gene            complement(950..1534)
                     /locus_tag="VI359_01695"
     CDS             complement(950..1534)
                     /locus_tag="VI359_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY6972994.1"
                     /translation="MVMIDECCRRTKAYLIIHAFCLVGILLSVWLLLGFFQEAFATTI
                     YSYVDERGTAVMTDNYNSIPERYRSKVKINESSPSSRPTGSPMANLHSSVTLLSKDLL
                     KGMTGLMPDIPGMTSYQSQILIYAGLMALVCVMAMYLSRGQVTRFLALWCLVLLGLAT
                     PVLLYVSKDGPVDVLRGKAGEIQEKQQDRLKNAQ"
     gene            1680..2105
                     /locus_tag="VI359_01700"
     CDS             1680..2105
                     /locus_tag="VI359_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972995.1"
                     /translation="MDTTTPAALEPTPILRQRPVRLTIYAGLGVFVLFMIGWPLVAQL
                     RDPNFQKHIAAHRVMVGMSKEQVLQAWGGPQTINTTFTKDGIRREEWIFEDWENTSVV
                     KHRYLYFEEGTLVGGWFQGSGERLPAQAPSNPHAPRSQS"
     gene            complement(2112..2342)
                     /locus_tag="VI359_01705"
     CDS             complement(2112..2342)
                     /locus_tag="VI359_01705"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY6972996.1"
                     /translation="MIRLTEPHSLGELVIVKSLLESNGIPFVVHHEHVSSLYPGVPFL
                     GTKVMIDSVDLSRAELLLSRLRLEVRDVSGQA"
BASE COUNT          574 a          680 c          597 g          560 t
ORIGIN      
        1 gggtagcgtc atgatactgg aaggaatcgt ggccagcaag caagagcggc gcttccacct
       61 tgacatgccc cctcctcgcc gatacgatgg ccgcacctgc tggagagaag ccttgctcct
      121 gcgcccaagc ccgtccatca actctcttcc aatttttctg cttgtattcg ttgtgtgtgc
      181 gtggagtgcc ccgttctctc ttatcgctgc accttatttt gaggacggct ttctcgggct
      241 gactcagcaa gagctgcacg agaagctcgg caagccgcag gccgtcagag atcgcaagtc
      301 cgcgctgcgc gtctttacct actattcgat ggcggattgg gaaaaatact tccggaaaat
      361 ggtttcgcct gagaatggcg aggatgtgta cacgttcaag cgagagggca tcgatatccg
      421 gtattcgttc agctacgcag tggatcccag cgaccaaaac gacaaccgca cgctttttgt
      481 ccgtctcgtg gatatcgagt tcgcacctcc ggttcccttg ggtcaggtac cttctctcgt
      541 ccctgagttc aagccatcgg aagaccctag tacgccagct tttcgttcaa acatttgggt
      601 cctgttattc aaaggcaccc cgtccgcgga agcgcgattt atcgtcaagg aaaaggggaa
      661 ggactctctc agttggtcgc ttgcctatca gctcttttcg cttcaagggc ttcccgatcc
      721 cttgaccacc aaagcgccgg tagatcgcat tgaaatcagc actcagagcg tcgatctcgt
      781 tagacagcgt cagcgtctca cccatgaccc tatcctcaat ccgtattcca aggaattcgc
      841 ccaacaacag ttggttccgc agcctccgaa aacaaaaaag atccccgtcc ctagctatgc
      901 ggactgatct tcaccgataa tgcagcgaga gaatacagaa ggaaggcagt tactgcgcgt
      961 tcttcagacg gtcttgttgt ttctcttgga tttctcccgc cttgccccgc aggacatcga
     1021 caggaccatc cttggaaacg tataacaaga ccggtgtggc gagacccaac aaaaccaaac
     1081 accaaagcgc aagaaatctc gtgacttggc cacgactcag atacatggcc atgacacaca
     1141 caagcgccat caaccctgca taaatcagga tctgcgactg atagctcgtc atgcctggaa
     1201 tgtctggcat tagtccggtc atgcctttca acaaatcttt cgacaaaagg gtgacggagc
     1261 tgtggagatt cgccatcggt gagccggtcg gacgagaaga tggtgacgac tcattgatct
     1321 tgacttttga tcggtatcgc tccggaatac tgttgtagtt gtctgtcatc accgccgtgc
     1381 cccgttcatc cacgtaggaa tagatggtcg ttgcaaaggc ttcttgaaag aagccgagaa
     1441 gaagccatac tgaaagcagg attcccacca ggcagaacgc gtgaatgatg aggtaggcct
     1501 tcgtccgcct gcaacattcg tcaatcatca ccataataga ttgagtatag cccttcttct
     1561 tatctccatg atttataggt ggattccgcc tctcgtcctt gactcccctc cgacaccgtt
     1621 gctagcatgc gacagactga ttcgaacctt cgtacgaacc gcctcgaggg atcatccata
     1681 tggatacgac gacacccgct gccctcgaac cgactcccat cctccgccag cggcctgtcc
     1741 gcctcacgat ttatgcagga ctcggggttt ttgtgctttt catgatcgga tggccgcttg
     1801 ttgctcaact gcgcgatccg aacttccaaa aacatatcgc ggcacatcgc gtcatggtcg
     1861 ggatgtcaaa agagcaggta ttacaggcct ggggaggacc tcagacgatc aatacgacat
     1921 tcacgaaaga cggcatccgc cgagaggaat ggatctttga agattgggaa aatacctcgg
     1981 tggtgaaaca ccgctatctg tacttcgagg aaggcactct tgtcggtgga tggttccaag
     2041 gatcggggga acgccttccg gctcaagctc cctccaatcc gcatgcacca cgatcacaaa
     2101 gctagggcgc gctaggcttg gccggacacg tctcgcactt ccaaccgcag ccgactcagt
     2161 aataattctg cgcgtgagag atccacggaa tcgatcatga ccttggtgcc aagaaaggga
     2221 acacctgggt ataagctgct gacgtgttca tggtgtacga caaagggaat tccattgctc
     2281 tccagcaagc ttttgacgat gaccaactct cccaaactat ggggctctgt caaacgaatc
     2341 atcgacattg attgcaccct acgatcagag acacctcgtg agcaagagcc tgcgatttcg
     2401 ttgcaatttg t
//
LOCUS       DATDNW010000064         2981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_620796, whole genome shotgun sequence.
ACCESSION   DATDNW010000064 DATDNW010000000
VERSION     DATDNW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620796"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VI359_01710"
     CDS             complement(<1..693)
                     /locus_tag="VI359_01710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY6972997.1"
                     /translation="MAEPEPNASQRPTQIDERGAGLALLPIAATLNFYILPEPLQTQT
                     LIQFIPQILSYLALALWAQHNTAFFPRLGLQPDGVRAGFRVGLITGLLLGSFNTFIIL
                     DIVPRLGEDISFLRDTPHAQIPVFIMIPWFICAIAVFVELNFRGFLLGRLLTLGLTMS
                     GALFRPCASAVAIGISAFVFAFDPFMMQTFRHLHWIALWDGVIWGILWVRTHNLYLTI
                     VAHAVEVIIMYSV"
     gene            complement(702..2261)
                     /locus_tag="VI359_01715"
     CDS             complement(702..2261)
                     /locus_tag="VI359_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6972998.1"
                     /translation="MNRWNTTKPGRLGFVLSFVLTLLPGPSQAAEDSKTAVPERREAL
                     SLADAALRALRSNLDISISRQTKESRLFDITVEQAKFDPTLSVNGQYTRTVSPLNRPV
                     FGGTTGNLTDIQIFDQRQSTVTVDATTNLLTGGNVDLNYSPSRTNVNQSVATGFLFNP
                     AYTGGLALTLTQPLLRNAGIELTKTFINIAQNNATVEEHVFRDRVLTVLATVEQTYWE
                     VVFANENLKVAEAALKAAQELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQVIVAEK
                     AIRDQEDQLRRLLNPAEEELRQDLRLTLLDQPAVALEPISLQEAIDIAIERRPEIVQA
                     KKNMETSNLNSKFAKNQLLPTLSFQGTMGLAGLGKDYSDSVNKNFSGDFYNYGAGLVL
                     SYPLGNRSAWSTFNKRQIEMKNAEASMVSVRQQIIIGVREAVRRVQTDFKRIETTRSA
                     RIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAILDYNKSLSNLARHKA
                     STLDRYDLQIQ"
     gene            2350..2760
                     /locus_tag="VI359_01720"
     CDS             2350..2760
                     /locus_tag="VI359_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6972999.1"
                     /translation="MVVRHVLPLLSGLLMLFVGAMHPLDHARGQTVSGVRSFDGGVGP
                     LFNLVGPGNLYLDNQGTQGYLYTPGVNFESYNFRNPTSGQAWSGAVMTLGPQLSIGLI
                     QGANQPGTGIVLPRPPLQTPPLLPIQSTVLEDIP"
BASE COUNT          635 a          804 c          861 g          681 t
ORIGIN      
        1 cacactatac atgatgatga cctcaacggc atgggcgacg atggtgaggt agaggttgtg
       61 ggttcggacc cacaggatgc cccagatcac gccgtcccag agggcgatcc aatggaggtg
      121 acggaacgtc tgcatcataa aaggatcgaa ggcaaacacg aatgcactga ttccgattgc
      181 caccgcggat gcgcagggcc tgaagagagc tcccgacatt gtaagaccca gggtgagcag
      241 acgtcccagg agaaaaccgc ggaaattcag ttcgacaaat acggcgatcg cacaaatgaa
      301 ccaagggatc atgatgaaga ctggaatctg ggcgtgcggc gtgtctctca ggaagctgat
      361 gtcctcacca agtcgcggca caatatccag gatgatgaac gtattgaagc tccccaacag
      421 aagtccggtg atcaggccaa cgcggaatcc tgcgcgcacc ccgtctggct ggagtcccag
      481 gcgagggaag aacgccgtgt tgtgttgggc ccacagagcc agcgcgagat aactcaggat
      541 ctggggaatg aactgaatga gggtctgtgt ctgtagcggc tcgggcagga tatagaagtt
      601 gagggtcgct gcgatgggaa gcagggcaag acctgctccc cgctcgtcga tctgagtcgg
      661 cctctgcgag gcgttcggct caggctctgc catggcggtc gctactgaat ctggagatcg
      721 tagcgatcca gcgtgctggc cttgtgtcgc gcaagattcg agagcgactt gttgtaatcc
      781 aggatcgcgc gtaattcgtt accctgggcc gtggccagat cacgctgaaa atccagaacg
      841 aagcgtgtcg tgctgagccc gaccttcagg cgttcttgct cggcctgcag ctgcttttcc
      901 gccatgatcc gcgcggaccg tgtcgtttca atccgtttga aatcggtttg gacccgtcgg
      961 actgcttcgc gcacgccgat gatgatttgc tggcgaacgc tgaccattga ggcttccgcg
     1021 ttcttcattt caatctgtcg tttattaaag gtgctccaag ccgaacgatt tcccaaggga
     1081 tagctgagga cgagaccggc gccatagttg tagaaatcac cactgaagtt cttgttcacg
     1141 gagtctgagt agtccttgcc caatccggcc aaacccatcg tcccttggaa cgacagcgtc
     1201 ggcagcaact ggtttttggc gaatttactg ttgagattgc ttgtctccat gtttttcttg
     1261 gcctggacga tttccggacg ccgttcgatg gcgatgtcga ttgcctcttg aagactgatg
     1321 ggttccaacg ccacggctgg ttggtcgagc agcgtcaatc gcagatcttg acggagctct
     1381 tcttcggcgg ggttgagcaa ccgacgcaac tgatcttcct ggtcgcgaat cgctttttcc
     1441 gccacgatca cttgttccac gcgtgatgcc acggcggcct ccgcttgcag cacgtcgacg
     1501 attgacatca caccggcttt cgccttggca cgattactgg cgagcagctc ctgcgcggct
     1561 ttcaaggcgg cttccgcaac cttcagattc tcgttggcaa agacgacttc ccaataggtt
     1621 tgctccacgg tcgccaggac ggtgagcaca cggtcgcgaa acacgtgttc ttccaccgtt
     1681 gcgttgttct gggcaatatt gatgaaggtc ttggtgagct cgatgccggc attccgcagc
     1741 aggggttgtg tcagagtcag tgccaatcct cccgtatagg cgggattgaa caagaatccc
     1801 gttgccacac tctggttgac gttggtccga gaaggactgt agttcaggtc gacattgccg
     1861 cccgtgagca aattcgtcgt ggcatcgacc gtcacggtcg attgacgctg atcaaatatt
     1921 tggatatccg tgagattgcc ggtcgttcca ccgaacaccg gtcggttcaa cggactcacg
     1981 gttctggtgt attgaccgtt gacactgagc gtcggatcga acttcgcctg ctcgaccgtg
     2041 atgtcaaata accggctttc cttcgtctgc cggctgatgc tgatatccag attgcttcga
     2101 agtgcccgca gagccgcatc ggccagcgag agggcttcac gacgttctgg tacagccgtc
     2161 ttgctgtcct ccgcagcctg gctcgggcca ggaagtagag tgaggacgaa actgaggacg
     2221 aaacccaacc gccctggttt tgttgtgttc catcgattca taatgccccc tttggtcgac
     2281 aacatgcctc ggatgatact ataatgttat ttggatagcc tgtcatgaac taagtgcgaa
     2341 gatgttggta tggtcgtgag acacgttctt ccattgctga gtgggttatt gatgctcttt
     2401 gtcggcgcga tgcacccatt ggatcacgcg cggggacaaa cggtatcggg agtgcggtcg
     2461 tttgacggcg gggttgggcc gctgttcaat ttagtggggc cgggaaatct ctacttggac
     2521 aatcagggca cccaaggata tctctacacg ccaggagtta atttcgaatc ctataacttt
     2581 cgcaacccaa caagcggcca agcctggtcc ggggccgtca tgactcttgg accacaactg
     2641 tcgatcggac tgatccaagg cgcaaatcaa ccgggcacag gcattgtatt gccgaggcca
     2701 ccactccaaa ctccaccatt actcccaatc caatctaccg tcctcgagga cattccgtaa
     2761 tatagggctg aggcaccggg ctggaaactg atgggagcga ccaccggtcc ccgcgcttgc
     2821 tgcgagccgg catcatggtt cattggcgag ttgccaggcg agtgcgagga catagcgggc
     2881 cgctgaaaca agaatctcct gattgagtgt ctcgaccgta tccgtcggtt gatgaaaatg
     2941 cagatgtgtt ccgccactca cgactgtgac agtgggaatc c
//
LOCUS       DATDNW010000065         4962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1236832, whole genome shotgun sequence.
ACCESSION   DATDNW010000065 DATDNW010000000
VERSION     DATDNW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236832"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..981
                     /locus_tag="VI359_01725"
     CDS             232..981
                     /locus_tag="VI359_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY6973000.1"
                     /translation="MTRPLTHNRFQEEILRIMDRKHHWAWPAFANGKMTKAQLRVHFQ
                     QEYAVYVRDFPVFLARIHGQNPPPSVRRMLAENIYEEDTGGLSLGKSHPELFLSMMEG
                     LGFQADSFDTIRLLPASRTYRAWLDRISGHRDWVIGAAALTIFVEGSVKDRQEILEPS
                     KPKTAEEIETTVQHHPLVRFHGLSPDHMDLIRSHQMVEAGHRHDAYDMVVGQATTRGR
                     QQAVLGCLKKSLKLWLAYRDAVATGCGLKKP"
     gene            981..1865
                     /locus_tag="VI359_01730"
     CDS             981..1865
                     /locus_tag="VI359_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY6973001.1"
                     /translation="MICRALGTLILFLTVSHHHAAISSGIPDDMALVPAGEFLMGSPA
                     DTDSLPDEQPQRRVYLSSYLIDRHEVTNEAYVTFVKTTGHRTPENANPSSTLWSDKIP
                     MAGIEHHPVVNVSWNDAAAYCQWAGRRLPTEAEWEKAARGIDAWRYPWGNDWDITKAN
                     SASYWAGRTIEFTSGANWEAFWVRGEGAKLAKEKGIKGEVLTMPVGSFPQSLSPYGLY
                     DMAGNVAEWVQDWYDPNYYRSASLSNPAGPERGAIKSMRGGSWLKPAVSLRTTDRDWG
                     TMDSRPSGTGFRCAQDAF"
     gene            2197..2526
                     /locus_tag="VI359_01735"
     CDS             2197..2526
                     /locus_tag="VI359_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973002.1"
                     /translation="MKYLLNLAFMVALGMILLSGETEAAQQTVNIRLAGKYCGFYQMD
                     LAKELKQIPGVTEVDFETMRGHVYVTMIAGKVHPNSLLAAIRRVKGDGYYCVGQFEGE
                     PGKIERY"
     gene            complement(2687..3346)
                     /locus_tag="VI359_01740"
     CDS             complement(2687..3346)
                     /locus_tag="VI359_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973003.1"
                     /translation="MKIFILGVLLIVSTSVATVRGQEVETTRPLLLSKSVDLETILPP
                     DTHLLIETPAIERFLAELDGIPPAWATVYGQGHHDPGHDDRLFSLNRERDELRQGKPA
                     LQWSIAFVWPGELSHYDSTTGGISVAVGPKFNPTRWGIVRFKPEDLPGNLLAVPDPGT
                     RATLLRELETKQAIDIVVVMTGRLIPQESIVYDFSHDEEGLGLIMPVVRVERIDYLLE
                     R"
     gene            complement(3357..3680)
                     /locus_tag="VI359_01745"
     CDS             complement(3357..3680)
                     /locus_tag="VI359_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973004.1"
                     /translation="MMEPGEEALQRAISTVSQSDPLIKLLEQVKLGRMQPADAGLRAI
                     TVSWLDTYRKVVETSGLSRQALRRLDPIPRLAVLIDEGVLPHDHQAVTSLQASFKSAL
                     HRATE"
     gene            3979..4245
                     /locus_tag="VI359_01750"
     CDS             3979..4245
                     /locus_tag="VI359_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973005.1"
                     /translation="MYQEEKTFTLRFSLEAAFPDEYDGDEDHQVWTRDWESRVKPDVI
                     KALFASLRQHPGWNAHIRNRGKSPVDEIEIVLSKDFSKPQSFNL"
     gene            4248..>4962
                     /locus_tag="VI359_01755"
     CDS             4248..>4962
                     /locus_tag="VI359_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEY6973006.1"
                     /translation="MSRTPLGLYLHIPFCRQRCDFCAFYLEIYRDGRARTFVDSLRRE
                     IRFYGTQDLAATHSIQSIYLGGGTPTTLSPDQLESILTDIRQTFVVLPDAEVSIEAHP
                     ATVSEQSLTRLIKAGFNRISFGAESMDDREFLSIGRPGTSQDTVRAVAMARAAGFTNI
                     NLDLMYGLPGQTVESWQQSLDRVVQLDPAHISCYALTIEEETKLAQNIERQATAAPDD
                     ARQVEMDAAADLLLRRAGFE"
BASE COUNT         1211 a         1406 c         1286 g         1059 t
ORIGIN      
        1 ttatagtagg cgaagggatc ttccccgctg gcattgagat aaatgggctc tgtgaagcct
       61 tcgtcgagga catagtccat gaggcattcg gacgtaaagc cgtctccacg caatgcgaca
      121 tcggctaaaa gggaaagaat ctcgtcgggg ttttgtacgg taatctgatt catgctacca
      181 cgctcgtagc ggcaaaagtt taactggcct catgagggaa ggcaagagac gatgacacgt
      241 cctttgactc acaatcgctt ccaagaagaa attctgcgca ttatggaccg taagcaccat
      301 tgggcatggc ctgcctttgc caacggaaaa atgactaagg cccaacttcg cgtccacttt
      361 caacaggaat atgcagtcta tgtccgtgac ttcccggtct tcctggcaag aattcatggg
      421 cagaaccctc cgccctctgt gcgccgcatg ttggcggaaa acatttacga ggaagacacc
      481 ggcgggttat cgctcggcaa gtcacacccc gagctgtttc tgtccatgat ggagggattg
      541 gggtttcagg ccgactcctt cgatacgatt cgtttgctcc cggcaagccg cacctaccgg
      601 gcttggctcg accgtatatc gggtcaccgc gactgggtga tcggcgccgc cgctctcaca
      661 attttcgttg aagggagcgt gaaggaccgt caggagatcc tcgagccatc caaaccgaaa
      721 acggctgagg aaatcgaaac cacagtccaa caccatcccc tcgtgcgatt ccacggccta
      781 tcacccgatc atatggatct catccgctcc catcagatgg tcgaggccgg ccatcgacat
      841 gacgcttacg acatggtggt aggtcaagcc acaactcgtg gtcggcaaca agcagtcttg
      901 ggttgtctca agaagagcct gaagctttgg ctggcgtatc gcgacgccgt cgccacgggc
      961 tgcgggctta agaagccctg atgatatgcc gagccctcgg tacgctgata ctcttcttga
     1021 ccgtcagcca tcaccacgca gccatcagtt caggcatacc cgacgacatg gccctggttc
     1081 ccgcaggcga attcttaatg gggtctcctg ccgatactga cagccttcct gacgaacagc
     1141 ctcagcgtcg cgtctatctg agcagttatc tcatcgatcg acacgaagtt accaacgaag
     1201 cttatgtcac attcgtcaag acgacggggc accgaactcc ggagaatgcc aatccgtcct
     1261 cgaccctttg gagcgacaag attccaatgg cagggattga gcatcacccg gtcgtcaatg
     1321 tgagttggaa tgatgccgcc gcctattgcc aatgggcggg gagacgcctc ccgaccgaag
     1381 ccgaatggga aaaggcagca cggggaatcg acgcgtggcg ttatccctgg ggaaacgatt
     1441 gggacatcac aaaagcaaat agtgccagtt actgggcggg gcgcacgatc gaattcacca
     1501 gcggcgctaa ttgggaagcc ttctgggtca gaggagaagg agcaaagctc gccaaagaaa
     1561 aaggcatcaa gggtgaagtg ctgacaatgc ctgtcggcag tttcccgcaa agcctcagcc
     1621 catatggtct ctacgacatg gccggcaacg tcgccgaatg ggtacaggac tggtatgatc
     1681 ccaattacta ccggtcggct tccctctcca atccagccgg ccccgagcgc ggcgctatta
     1741 aatccatgcg aggcggatcc tggctgaaac cagcggtgag cctgcgcaca actgaccgtg
     1801 actggggcac gatggatagt cgccccagcg gaactggatt tcgatgtgct caggatgcgt
     1861 tttgatgtcg tgttctgctg gctgatttgt cacaaccttc ctttcttcca aacgaatcct
     1921 cctaccccga gagagagagt tacacggcca gtttattaac agaacgacac attccactgc
     1981 cgcctcaaga acaaaagagc ggatgcgcac agaccgctca gccaaaatct atcaaataga
     2041 tcaatcagtt acatgggatc acgccagaaa gaatgccacc tcatttacca aagccgttac
     2101 caagacaaat accaaaaacg tggtagattt tttagttagg aggtctggat cccacgcggc
     2161 cccaaaagag cgtggaatca gcaggaggag gtccttatga aatacctatt gaatctcgcc
     2221 tttatggttg ccctcgggat gattctttta tcaggcgaaa ccgaggcggc acagcagaca
     2281 gtaaatatca gactcgccgg aaagtactgt ggattttatc aaatggatct tgccaaggaa
     2341 ttgaaacaga ttccaggcgt cacggaggtg gacttcgaaa cgatgcgagg gcatgtctat
     2401 gtaaccatga tagccgggaa ggtacatccg aactccctgc tcgctgcaat ccggcgtgtc
     2461 aagggagacg gatactactg tgtaggccaa tttgaaggtg agccaggaaa aattgagcgt
     2521 tactgaaggc tgacagaaag aactgcccgc tactatatgt aggcggacgt tctacgaact
     2581 ctccctcaga ccgcgtgcta ttgctcctga caacataact gatttcaccg cttgtctctc
     2641 gcgcgaaacc taccataacg tcgaatgctg atcatcactc agcaattcat ctttccagca
     2701 gatagtcaat tctctcaact cgcacaaccg gcatgataag cccgagacct tcttcgtcgt
     2761 gagaaaagtc gtacacgatc gactcttgag gtatgagccg acccgtcatg accacaacga
     2821 tatcgatcgc ctgctttgtc tccagttcac gtaacaaggt tgcccgcgtc ccaggatccg
     2881 gaacggccaa aagatttccc ggcagatctt ccggtttgaa ccggacaatt ccccagcgcg
     2941 tgggattaaa cttcgggcca acggcaacgc tgattccccc ggtcgtagag tcataatgag
     3001 acagttcgcc cggccacaca aacgcgatag accattgcaa cgcaggtttt ccctgtcgca
     3061 gctcatcccg ttctcgattc aggctaaata accgatcatc atgacctgga tcatgatgcc
     3121 cctgcccata gaccgttgcc cacgctggtg gaatgccatc gagttcggcc aagaatctct
     3181 caatcgccgg ggtctcaatc aggagatgtg tatcaggagg aagaatagtt tcaagatcga
     3241 ccgacttgct caatagaagc ggccgtgtcg tctcaacctc ttgcccccgg acagtcgcca
     3301 cactcgtaga aacgatcagc agaactccga gaataaagat cttcacagct atgccatcat
     3361 tcggtggcac ggtggagggc ggatttaaaa ctggcttgga gcgatgtgac ggcctggtga
     3421 tcgtggggaa gcactccctc gtcaatgagg accgccagtc gaggtatagg atcaagcctg
     3481 cgaagagctt gccgcgacag cccgcttgtc tcgaccactt tccgataggt atctaaccac
     3541 gagactgtga tggcacggag ccctgcatcg gcaggctgca tccggcccaa cttcacctgt
     3601 tcgagcaatt tgatcagggg atcagactga gaaacggtgc tgatcgcacg ctggagagct
     3661 tcttcgcccg gctccatcat gcttcaattc atggagcagg agcctggtcc gcgaggatct
     3721 ccgcaggtcc cacaggaacc gggtccgctc gacgtcgccc aacttaccgt ggctccaacc
     3781 ccgaaggcgg agaattgttt cgcgagcttg gtagctttgc acttcgggca ggctgccacc
     3841 gtcgaaccct gcaccagcaa ttcgaatcga tgattgcaat cctgacagac atattcgaag
     3901 ataggcatcg caacgctcct tcgtaatgcc atattatagg atcgtggccg acgctggcgc
     3961 aacgcccctc gaggaattat gtatcaggaa gaaaagacat tcaccctccg cttcagcttg
     4021 gaagctgcgt tccccgatga gtacgacggc gatgaggacc accaggtgtg gacccgcgac
     4081 tgggaatcac gggtgaaacc tgacgtcatc aaggcactct ttgcctcgct gcgacaacat
     4141 ccgggctgga atgcccacat ccgtaatcga ggcaaatctc ccgtcgatga aatcgaaatt
     4201 gtgctgtcga aagacttctc caaaccgcaa tcatttaatc tctaaacatg tcgcgcacgc
     4261 cgctcggtct ctacctccac atcccgtttt gtcgccagcg atgcgatttc tgcgccttct
     4321 atctggaaat ctacagggac ggtcgcgccc ggacatttgt agattccctg cggcgcgaga
     4381 tccgtttcta cggcacacag gaccttgcag cgactcattc gattcaatcc atctaccttg
     4441 gcggcggaac acccacgaca ctctcacccg atcaattaga gagtattctg accgatatcc
     4501 ggcagacatt cgtcgttttg cccgatgcgg aagtatcaat cgaggcccac cccgcgactg
     4561 tctcggagca gagtctcacc cgcttgatca aggccggatt caaccggatt agtttcggcg
     4621 cggaatccat ggatgaccgg gaatttcttt caatcgggcg acctgggaca tcccaagata
     4681 cagtccgtgc agttgcaatg gcccgcgccg caggatttac aaatatcaat cttgatctca
     4741 tgtacggttt acccggtcaa accgtggaga gctggcaaca atctctcgat cgtgttgttc
     4801 agctcgatcc cgcccatatt tcctgttatg cgttgacgat cgaagaagag acgaagctgg
     4861 ctcagaacat cgagcgacag gctactgcgg cccccgatga cgcacgacaa gtcgagatgg
     4921 acgccgcagc tgacctgtta ctccgccgag ccggttttga ac
//
LOCUS       DATDNW010000066         4022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_552805, whole genome shotgun sequence.
ACCESSION   DATDNW010000066 DATDNW010000000
VERSION     DATDNW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552805"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VI359_01760"
     CDS             <1..429
                     /locus_tag="VI359_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY6973007.1"
                     /translation="GEPLAQPGCFPFISKLCDAGYTALIETSGAIDITTVDPRARVIL
                     DIKCPGSGMTDRMHWPNVQKLTAKDEAKFVLTNRADYEWARDIIAQYDLANRCTVLFG
                     PVFGTLELRQLAEWVLADRLPVRLQTQLHKLIWTPDMRGV"
     gene            495..2006
                     /locus_tag="VI359_01765"
     CDS             495..2006
                     /locus_tag="VI359_01765"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY6973008.1"
                     /translation="MNIMRVEAKVGRAESEPAEILVLLHCEGDGLAKMDAAPLDKMLG
                     GALRGLIHSREFEGKANEVLLYHTQGKVPAKRFVLVGLGKKKDVTLDSLRQALGHAVK
                     RVRQAKVGTFMVALPTVMPKSSTLVEVVQVMVEGAILGNYQFTTYRSEKGPSNDVSGM
                     TILTSQKTQLRQLIEGIRRGVATAEATVLVRDLCNHPSNVMTPTRIANEAKAVAKEPG
                     VSLKILEQKDMEQLGMGALLGVARGSHEPPKFIILEYNRAKKKDERPVVLVGKTITFD
                     TGGISLKPAENMEHMKADMTGGAEVLASMRAAARLKLPLRLISILPVAENMPGGRAMK
                     PGDIVKTLSGKTVEVQNTDAEGRLILSDALAYAQRYKPAALIDIATLTGACVVALGQF
                     AIGMFGTDGKLKDAVRKAGLRAGERVWEMPLWDEYFEQLRSDVADMRNIGGRGGGMIT
                     AALFLSKFVGDCPWIHLDIASTDWSERERAYIPKGPTGIGTRLLIQCLIDRSL"
     gene            2008..3135
                     /gene="nagZ"
                     /locus_tag="VI359_01770"
     CDS             2008..3135
                     /gene="nagZ"
                     /locus_tag="VI359_01770"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY6973009.1"
                     /translation="MLGRSLSSRDKIGQLFMVGFTGTSVTSDLASFIKEYKPGGLILF
                     SRNLESVDQIVELTNDLQRCSPHSPLLIAIDQEGGRVSRLPKGFTIFPPCHVLGCCNS
                     TELAYAAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGSAPNIVSELGWAT
                     AAGLQDHQVIACGKHFPGHGDTNADSHKELPVVEAARERLERIEFPPFRHAVARGVAT
                     IMTAHVLYRALDEKLPATLSSTIITKLLRHDMRYDGVVFTDDLEMHAIIDHYGIADAA
                     VRAVLAGCDVLLICKDRDREVAAFTAIEEAVALGNISMERLDQSAARIARIKEHFVTP
                     YRPALISDAKLIVGCRTHQALLHSIQQAQGRYEQSAASAEA"
     gene            3168..3554
                     /locus_tag="VI359_01775"
     CDS             3168..3554
                     /locus_tag="VI359_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973010.1"
                     /translation="MSLKKGDLIDEHKRHPHSCGLVVKVMSGGAGFEKIVCCDHELTD
                     EDRVSEMGRPTGRKRGALPIAVIIDEKLLYPDSCGLRVMIMDGGAGFQEIRCCGHSLT
                     TSSVKELKYNPMSAPEPRETGPSGTA"
     gene            3576..>4022
                     /locus_tag="VI359_01780"
     CDS             3576..>4022
                     /locus_tag="VI359_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEY6973011.1"
                     /translation="MQNRKEVMKRWCGVMEWLDRLGYLTAGFSLLVLGMLIFAHAWYA
                     FVMKAGQVGLLQSGLKLLNDLLLVIILLELFRTVIRFLQTEILDLEPYLAVGIIACTR
                     RILTASAELSHLPAMTDTQFYQYLMDVGLNVTVIMVLVVGVFLVRKR"
BASE COUNT          881 a         1095 c         1222 g          824 t
ORIGIN      
        1 ggagaacctt tggcgcagcc gggatgtttc cccttcattt ccaagctctg cgatgccgga
       61 tacacagctt taatcgaaac cagcggcgcg attgatatca cgaccgtgga tcctcgcgcc
      121 cgtgtaattc tggacatcaa atgccccgga agcgggatga cagatcgtat gcactggccg
      181 aatgtgcaga aactcaccgc gaaagatgaa gccaaatttg tcttgacgaa ccgggccgac
      241 tacgagtggg cgcgcgatat catcgcccaa tacgatctcg cgaaccgctg taccgtgttg
      301 tttggccctg tgttcggcac gctcgagctt cgccagctgg ctgagtgggt gctcgcggat
      361 cgattgccgg tgcgactgca gacgcaactg cacaagctaa tttggacgcc ggatatgcgg
      421 ggtgtctgac agagccatcg gcgcggagac gccagtgatc gggttctgct gaaggcttga
      481 acaaggagac gtgaatgaac atcatgcgag ttgaggccaa agtcggacga gcggaatcgg
      541 aaccagccga aattctcgta ctcctccatt gtgaaggaga cgggctggcc aagatggacg
      601 cagccccgct tgacaagatg ctgggtggcg cgttgcgggg ccttattcac tccagggaat
      661 tcgaaggtaa ggcgaacgag gttctgctct atcacactca ggggaaggtc ccggcgaagc
      721 gattcgtgct tgtcgggctc gggaagaaaa aggatgtaac gctcgatagc ctccgccagg
      781 ccctgggcca cgcggtcaag cgagttcgcc aggccaaggt cgggacgttc atggtggcgc
      841 ttccgaccgt gatgccgaag agtagtactt tggtcgaggt cgtccaggtg atggttgagg
      901 gcgcgatcct ggggaactat cagttcacca cctaccgtag cgagaaggga ccgtccaacg
      961 acgtgagcgg gatgaccatc ctgacatctc agaaaaccca gctgcggcag ctcatagagg
     1021 gcatcaggcg gggcgtggcg acggctgagg cgactgtgct ggtccgggac ctctgcaatc
     1081 atccgtccaa cgtcatgacg ccaacgagga tcgccaacga agccaaggcg gtcgccaaag
     1141 aaccgggcgt cagtctcaag attctggaac agaaagacat ggaacagttg gggatgggtg
     1201 cattactagg tgtggcgcgt ggcagccacg aaccacccaa atttatcatc ctcgaataca
     1261 accgagccaa gaaaaaggac gaacggccgg ttgtgctcgt gggcaaaacc attaccttcg
     1321 acaccggcgg gatatcgtta aagccggcgg agaatatgga acatatgaag gcggacatga
     1381 cgggcggggc cgaggtctta gcgtccatgc gggctgcggc gcggctcaaa ctcccgctcc
     1441 gactgatcag cattctgccg gtggccgaga acatgccggg cggtcgagcg atgaaaccgg
     1501 gcgacatcgt gaagacactg tccggaaaaa ccgtggaggt tcagaacact gacgccgaag
     1561 gccgcctgat cctgtccgat gcgctggcct atgcccagcg ctacaaaccg gcggcattga
     1621 tcgatatcgc gacactgacc ggggcctgtg tcgtggcgct cgggcagttt gccatcggca
     1681 tgttcggtac agatggcaaa ctgaaagacg cagtgcggaa ggccggtctc agggccggcg
     1741 agcgtgtctg ggaaatgcca ctctgggacg aatacttcga acaattgagg agcgatgtgg
     1801 cggacatgcg caacattggg ggccgtggag gcggcatgat cacggccgcg ctctttctca
     1861 gcaagtttgt cggagattgt ccctggatcc atttggacat cgcgagtacg gattggagcg
     1921 agcgggaacg ggcgtacata ccgaaggggc ccaccggcat cgggacccgg ttactgatcc
     1981 aatgtctcat cgatcggagt ctctaaggtg ctcgggcgct cattgagctc gcgggataaa
     2041 atcggccaac tctttatggt cggattcacg ggaacctccg tgacatccga cctagcttcg
     2101 ttcatcaagg agtacaaacc gggcggcctc attcttttct cgcggaatct ggaatctgtc
     2161 gaccaaattg tcgaactgac aaacgatctc caacgctgca gcccccattc cccgcttttg
     2221 attgcgatcg atcaagaagg cggtcgagtc tctcgactcc ccaagggctt taccattttc
     2281 ccgccctgcc atgtgctggg ttgttgcaat tcgacggaat tggcctatgc agccgcggcg
     2341 acgattgcga aagaactccg agcggtcggg atcaatatga atatggcccc ggtgctcgac
     2401 gtcaacagca atcccgacaa cccggtcatc ggggaccgtg cctttggctc agccccgaac
     2461 atcgtgtctg agctggggtg ggcaacggca gctggactcc aagaccatca ggtcatcgcc
     2521 tgcgggaagc attttcccgg ccatggcgac accaacgccg attctcacaa ggaactcccg
     2581 gtcgtggagg ctgcacgcga gcgcttggag aggatcgagt tcccgccgtt tcgccatgct
     2641 gtcgctcggg gtgtcgccac gatcatgacg gcccatgtcc tctaccgtgc cctcgacgag
     2701 aaactcccgg ccaccttgtc atcgacgatc attacgaagt tattgcgtca tgacatgcgg
     2761 tacgacgggg tggtttttac ggatgatctc gaaatgcacg cgatcattga tcattatggt
     2821 attgcagatg cggcagtccg tgcggtctta gccggctgcg atgtcttgtt gatctgcaag
     2881 gatcgtgacc gggaagtcgc tgcgtttact gcgattgagg aggcagtggc cttagggaac
     2941 atttcgatgg aacgactgga ccaatcggcc gcgcgcatcg cacgaatcaa agagcacttt
     3001 gtgactcctt accggccggc cctcatctcg gacgcgaagt tgatcgtcgg gtgccgaacc
     3061 caccaggcct tgctccattc gattcagcag gcacaagggc ggtatgagca gtcggcggcg
     3121 tcagcggagg catgacgaac cagttctttt cctgatcagg agacgcgatg agtttgaaaa
     3181 agggcgattt gatcgatgaa cataagcggc atccgcattc ttgcggatta gtggtcaagg
     3241 tcatgagcgg cggggcgggc tttgaaaaaa tcgtctgttg cgaccatgag ctgaccgatg
     3301 aggaccgcgt ttccgagatg ggacggccca ccggtcgaaa aaggggggcc ttgccgatag
     3361 ctgtgatcat cgatgagaag ctcctctatc cagattcctg cggtctgcgc gtgatgatca
     3421 tggacggtgg cgcgggattt caggaaatcc gctgttgcgg gcattccctc acgacgagtt
     3481 ccgtaaaaga acttaaatac aatccaatga gcgcacctga gccaagagaa actgggccgt
     3541 cgggcaccgc ctaaccgtag caaacggagg gacccatgca gaaccgaaaa gaggtcatga
     3601 agcgctggtg cggggtgatg gaatggctcg accgactcgg ttacctcact gctggcttca
     3661 gtctgctggt cctcgggatg ctgatctttg cgcacgcctg gtacgccttt gtcatgaagg
     3721 ccggtcaggt tggcctcttg caatcgggcc tgaagctgtt gaacgacctg ttgcttgtca
     3781 tcatcttgct cgaactgttc cgaaccgtga tcagatttct ccagacggaa attctcgatc
     3841 tcgaacctta tctggctgtc gggatcattg cctgtacgag gcgaattctc accgccagcg
     3901 cagaactctc ccatctccca gccatgaccg acacgcagtt ttatcagtac ctcatggacg
     3961 taggtttaaa cgtcaccgtg atcatggtac tcgtcgtggg cgtcttcttg gttcgcaaac
     4021 gc
//
LOCUS       DATDNW010000067         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_485986, whole genome shotgun sequence.
ACCESSION   DATDNW010000067 DATDNW010000000
VERSION     DATDNW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485986"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..870)
                     /gene="gmk"
                     /locus_tag="VI359_01785"
     CDS             complement(172..870)
                     /gene="gmk"
                     /locus_tag="VI359_01785"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY6973012.1"
                     /translation="MTTQPSSSAPETSRHSADRRGILFIISAPSGTGKTTLCKQLTST
                     VPGLWHSISYTTRKPRPGEEHGREYFFIEESEFQQMVDRNEFVEWARVYGNLYGTPRK
                     ILTDQIEQGIDVLLEIDVQGAIQVKKKFEDAVAIFILPPSMHVLRSRLQNRASDTPEE
                     ILRRLQKVKEEVWSYREYAYVVRNEDLSQSLRELESVFLAERLKTKRLNMAWLEKNFI
                     LEDESKHGDRRDLP"
     gene            complement(879..1757)
                     /locus_tag="VI359_01790"
     CDS             complement(879..1757)
                     /locus_tag="VI359_01790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY6973013.1"
                     /translation="MIRSMTGFGRRQAAWQDGSVTVEVRSVNHRFLEVACRLPRSLNH
                     LEEAFKKAVQQRCTRGRIDLTVSVQGGKGRAVTVSLDQSLAKQYHQTLRALKKSLKLS
                     GSIDLALIAGLRDVVSVSDQPPEDPKLGKLVVRLVAESLHDLAGMRSREGTALAKDML
                     TRLQVIGDHRLRVAARAPHLVRDAFDRMKSRVEKLLGEAVPDLARLNQELAIFADRAD
                     ITEELVRLDSHMIQFQQTLEHTEPVGKTLDFLLQEIGREVNTIGSKANDAEIAVHVIQ
                     MKAEIERIREQVQNIE"
     gene            complement(1754..2407)
                     /gene="nth"
                     /locus_tag="VI359_01795"
     CDS             complement(1754..2407)
                     /gene="nth"
                     /locus_tag="VI359_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY6973014.1"
                     /translation="MKVSAVRAQSRMRQILDALQQSVPTVKVELDHRTPWELLVATIL
                     SAQCTDQRVNQVTPNLFRRYRRPSDYAQARLADLETLIRSTGFFKSKAKHLMACGQAV
                     VDRFDGQVPATMEELTTLPGVGRKTANVILGNAFGKPGVVVDTHVKRVSTRLGLTTSD
                     DPEQIEHDLQQLIPQSQWTTASQRLLLHGRYVCVARKPHCPQCSISSLCHWKGKQVL"
     gene            complement(2441..3970)
                     /gene="hflX"
                     /locus_tag="VI359_01800"
     CDS             complement(2441..3970)
                     /gene="hflX"
                     /locus_tag="VI359_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY6973015.1"
                     /translation="MIARRGTIQDVLVGADAGPTATTLAKYRTSSRSLRGLRLIRTQL
                     HGQPLSQEDITMLALLRLDMIGALAVTPEGEPGVLSLAHLLPPNPQGQLCKVLSPTLV
                     HHCPIEFHSFMQELESELLRESAQHDVAGHQETAILVSASSHSRVTQEDRLAELAELA
                     TSAEISVVDRIVQRTQIGHHRFLVGSGKLKDVLMRALQKGADLLIFDQDLAPAQARAI
                     AEFTDVKVIDRTQLILDIFARRAHSREGKVQVELAQLRYLLPRLSGHGTSLSRLGGGI
                     GTRGPGETKLETDRRRIRERISHLERELTVFARHQNQRRARRVRHALPVVSIVGYTNA
                     GKSTLLNALTKSQVAADDRVFETLDTTSRRLRFPHDQEVIITDTVGFIRDLPKDLLGA
                     FRTTLEELREADILLHVVDASAADLDSQIAAVETVLQDLNLDQVPQVLAFNKCDRIPD
                     DHVHFLCRRYRAIGISAVQPETLHPLIHHLEQTLEQLRSDTDTVSTQPSNEIAALASH
                     T"
     gene            complement(4165..>5095)
                     /gene="tsaD"
                     /locus_tag="VI359_01805"
     CDS             complement(4165..>5095)
                     /gene="tsaD"
                     /locus_tag="VI359_01805"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY6973016.1"
                     /translation="NVISSQHAVHQPFGGVVPELSSRAHIETIEGVANQAMREAGVQW
                     TDLAGLAVTRGPGLAGSLIVGVNYAKALSYALHIPMVGVSHLEGHIASAWLQDPAFPV
                     PCVVLVVSGGHTHLYLKQIDAGCALLGRTRDDAAGEAFDKGAQMLGLEYPGGPAIDRL
                     AQTGSATAIPFPRSYLMKESLDFSFSGLKTALLYKLQRMDQGLRDARIADLAAGYQEA
                     IVTVLVDKAFFAVKSYEVTALAVVGGVSANTRLRVLLQRRANTSGIRLSLPPLSYCTD
                     NAAMIAAAGRQALLMGKRAGLDMEADACLALQA"
BASE COUNT         1113 a         1377 c         1442 g         1163 t
ORIGIN      
        1 gtggtttctt tcgtgaagcg ggaagtcccg tgagaccgcg atccctgcaa gagatgcttg
       61 gcgcgttggg ctgccaccag caccagccgg tgacgcgaat cgaattcatc cgtcgtgtat
      121 tgtgggagaa ggcttaacat atcaaccata gatcatctgc tcctttgaaa gttaagggag
      181 atccctacga tctccgtgtt tggattcgtc ctccagaata aaattcttct caagccaggc
      241 catgttcagg cgcttggttt tcagccgttc ggcaagaaac acactttcga gttcgcgcag
      301 ggattgggaa agatcttcgt ttcgaaccac ataggcatat tcgcgatagc tccagacctc
      361 ttccttcact ttttgaagcc gtctcagtat ctcctccggc gtatcggatg ctcggttttg
      421 caaccgggac cgaagcacgt gcatcgacgg aggaagaata aaaatcgcta cagcatcttc
      481 gaactttttc ttcacttgta tagcaccctg cacgtcgatc tctaagagca cgtcgatccc
      541 ttgctcgatt tgatcggtca atatcttgcg gggtgtgccg tagagattgc cgtacaccct
      601 ggcccattcg acgaattcgt ttcgatcgac catctgctgg aattcacttt cttcgatgaa
      661 gaagtactcg cggccatgtt cttctcctgg acgcggtttc cttgtggtat aggaaatcga
      721 atgccagagt cctggcacgg tcgatgtcaa ttgcttgcag agtgtggttt tcccggttcc
      781 ggatggagct gaaatgatga aaagaatccc acgccggtca gcggaatgtc gcgacgtttc
      841 aggcgctgac gaggaaggct gtgtggtcat agcaggcgtc attcaatatt ttggacttgt
      901 tcacgaatac gttcgatctc ggccttcatc tggatgacgt gcacggcgat ctctgcatcg
      961 ttcgcttttg agccgatcgt gttgacctcg cggcctatct cctgaagcaa gaaatcgagc
     1021 gttttcccaa caggttccgt gtgttcaagc gtttgttgaa actgtatcat atgtgagtct
     1081 aatctgacca attcttctgt aatgtcagcc cgatcagcaa agatcgccaa ttcctgattg
     1141 agccgggcga gatcaggaac ggcctcaccc aaaagcttct ctacacggct tttcatccgg
     1201 tcgaaagcgt cccgcaccaa atgtggcgct ctggccgcga cgcgtagccg atggtctcca
     1261 ataacctgga gcctcgtgag catgtcttta gccagggccg tcccttcccg cgatcgcatt
     1321 cccgcaaggt catgcaatga ttctgctacc aatcgcacga ccagcttccc caatttcgga
     1381 tcttccggtg gttgatcgga tacggagaca acatcacgga gcccggcgat gagggccaga
     1441 tcgattgagc cgctcagctt caaggatttc ttaagcgcgc gaagggtttg atggtactgc
     1501 tttgccagcg attggtcaag gctgacggtc acggctctgc ccttccctcc ttgcacagaa
     1561 acggtaaggt cgatccgccc gcgagtacag cgttgttgga ccgccttctt gaacgcctcc
     1621 tccaggtgat tcagcgaacg tggcaggcga caggctactt cgagaaaacg gtggttgacg
     1681 gatcggactt ctaccgtcac ggacccatct tgccaggcag cctgtcgacg gccaaatccg
     1741 gtcatgctgc gaatcataga acttgctttc ctttccagtg gcagagggag gagatgctgc
     1801 attggggaca gtgaggtttg cgcgcgacac aaacataccg accatgcagg agcaggcgct
     1861 gcgatgcagt ggtccattgg gattgcggaa taagttgctg caaatcgtgc tcaatttgct
     1921 cgggatcatc agaggtggtt aaaccgagtc gcgtcgaaac tcgtttgaca tgggtatcaa
     1981 caacaacgcc cggtttcccg aacgcatttc cgaggatgac attggcggtt ttccttccta
     2041 cacccggcag ggtcgtcagt tcttccattg tggcagggac ctgtccatca aatcggtcca
     2101 caaccgcctg accacaagcc ataagatgct tagctttgct tttgaagaaa ccggtcgatc
     2161 gtatcaatgt ttcaaggtcc gcaaggcgtg cctgcgcgta gtcggacggc cggcgataac
     2221 gacgaaagag attcggcgtc acttgattca cgcgttgatc cgtgcattga gcggaaagga
     2281 tcgtcgcgac caacaactcc catggtgtgc gatggtcgag ttcgaccttc accgtcggga
     2341 cggactgctg gagtgcatcg agaatctggc gcatgcgcga ctgagcacga acggcggaaa
     2401 ccttcatcga aatcaccggt ggagctggag tgtgcagcag ttaggtgtga gaagcaaggg
     2461 cggcgatctc gttggacggt tgggtggaaa ccgtatcggt atccgatctc aactgttcaa
     2521 gtgtctgttc cagatggtga atgagcggat gaagggtctc cggctgaacc gccgagattc
     2581 cgatcgctcg ataccggcgg cagagaaagt gaacatgatc gtcgggaatg cggtcgcatt
     2641 tgttgaatgc aagcacctga ggaacctgat cgagattcaa gtcctgcagg accgtttcga
     2701 cggcggcgat ttggctatcg aggtcggcag cgctggcatc cacaacgtga aggaggatat
     2761 cggcctctcg caactcttcc aaggttgtgc gaaaagcgcc aagtaggtcc ttcggaaggt
     2821 cgcggataaa tccaaccgtg tcggtgatga tcacttcctg atcatgtggg aagcgcaggc
     2881 gccggctggt cgtatccaaa gtttcaaaga cccgatcatc agccgcgact tggcttttgg
     2941 tcaacgcgtt caataaggtc gatttgcccg cgttcgtata tccgacaatc gagacaaccg
     3001 gcaacgcatg tcggacacgg cgcgcccgcc gttggttctg atggcgagca aagaccgtca
     3061 actcgcgctc gaggtggctg atccgctctc gaatccgccg gcgatccgtt tccaactttg
     3121 tttctccggg gcctctcgtg ccgataccgc cgccaagtcg tgacaggctt gttccatggc
     3181 cggaaagacg cggcagtaag tagcgaagtt gtgcgagttc cacttgaacc ttgccttcac
     3241 gactgtgggc gcgcctggcg aaaatatcaa gaatcaattg ggtgcggtca atgactttga
     3301 cgtcggtgaa ttccgcaatc gctcttgcct gggccggggc gaggtcttga tcaaagatca
     3361 ggaggtcggc ccctttctgc agggctcgca tgaggacatc tttgagtttt ccgctgccta
     3421 cgagaaagcg gtgatggccg atttgcgtcc gttgaacgat ccgatcgacg accgaaattt
     3481 ctgcggatgt ggccaactcg gcgagttcag ccaaccggtc ttcttgggtc actcgactgt
     3541 gggaggaagc gctcacgagg atggcggttt cctgatgacc tgccacatca tgctgtgcgc
     3601 tctcccgcag aagctccgat tcaagctcct gcatgaagga atggaattca atcggacagt
     3661 gatgaaccag cgtcgggctc aacaccttgc agagttgccc ttgggggttg ggaggcagta
     3721 aatgcgcgag ggacagcact cctggttcac cttctggggt cacggccaat gcgccgatca
     3781 tgtcgagccg gagcaacgcc agcatggtga tgtcttcttg gcttaggggt tgcccgtgga
     3841 gttgagttcg aataagtctg agcccccgca aagagcggga actcgtccgg tatttggcca
     3901 aggttgtggc agtaggccct gcatcggctc ccaccaacac atcctggatg gtcccacgac
     3961 gggcaatcac caacccaatc gggcgtcgaa gatcatgact gagctgaacg agaagtttgg
     4021 caacttccgg cgagagaact ttatccgcag gaacacgccg tcgatagagg cgatctagtg
     4081 cggcgagctg gccggcccgg agacctgtga cctgtccatg aacttgtgga atcgttcaat
     4141 ctcccaattt cgtacccatg cggttcaggc ctgcagcgcc aagcatgcgt cggcttccat
     4201 atctaggccc gcacgtttgc ccatgagtaa ggcctgccga ccggcagcgg caatcatggc
     4261 agcgttatcc gtacaatagg acaacggagg caggctcaga cggatgccac ttgtgttggc
     4321 gcgccgctgg agaagcacac gcaacctcgt attggcagaa acgcctccga cgaccgctag
     4381 ggccgtcacc tcatatgact tgaccgcaaa aaaagctttg tcgacaagaa cagtgacaat
     4441 tgcctcttgg tatccggctg ccagatcggc gatgcgagca tcgcgcagac cctgatccat
     4501 tcgttgaagt ttgtagagaa gcgccgtttt gagaccgcta aaactaaaat ccagactctc
     4561 cttcatcaaa tacgagcggg gaaacggaat tgccgtggca ctgcctgttt gtgcaaggcg
     4621 atcgattgcc ggacctccag ggtactcgag tccgagcatt tgagctcctt tgtcgaatgc
     4681 ttcccccgcc gcatcgtctc tggtgcgtcc caagagtgcg cagccggcat cgatctgctt
     4741 cagatacaga tgggtatgac ctcctgaaac aaccaaaacc acacacggga ccgggaatgc
     4801 tggatcttgc agccaggcag aggcaatatg gccttccaag tgactgactc cgaccatcgg
     4861 aatatgcaag gcgtaggaaa gtgctttggc atagtttact cccacgatga gggatcctgc
     4921 gagtcctggt cctcgggtga cagctaagcc ggccaggtcc gtccactgga cgcccgcttc
     4981 acgcatcgct tgattggcga caccctcgat ggtctcgata tgggcacgtg aggagagttc
     5041 aggcaccaca ccgccaaacg gttggtgaac ggcatgttgc gagctgatga cgttg
//
LOCUS       DATDNW010000068         3926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_145701, whole genome shotgun sequence.
ACCESSION   DATDNW010000068 DATDNW010000000
VERSION     DATDNW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145701"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VI359_01810"
     CDS             complement(<1..1263)
                     /locus_tag="VI359_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY6973017.1"
                     /translation="MGASTTAKRGDARRASSPPSHLKREVIGVVLIALSLLTLLSLVS
                     FVPGEGKVLSGSLAPPPRNLIGSVGALFASSLFFLIGGAAYLFPLLLGLIGVRCFTQS
                     TLSIRIRNGAASLGVLLFLSGFLHLEVTAVPTVSSGFVYRGIAGGICGQVLAEGLRTY
                     FASTGAHILIIAGFLVSMLYTTPVSLAQLALRLSEWWSRAVSRISALMPERGPAPELE
                     APVKKAKAKAAKSIRSVIEEVLPATEPSPVVDSSVAQPLPKPVEEAPEPLETEPEPQI
                     SAAQDEADDYVLPDPAELLSEPSGALARMTEEELKAQSEILARALLSFGIEGKVTDVR
                     PGPVVTMYEFEPAPGTKVARIVNLADDLALAMKAISLRIVAPLPGKSVVGIEVPNPYR
                     ETVSLKDVVTSEVFTRARSKLTLALGKDI"
     gene            complement(1340..2131)
                     /gene="rsmA"
                     /locus_tag="VI359_01815"
     CDS             complement(1340..2131)
                     /gene="rsmA"
                     /locus_tag="VI359_01815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY6973018.1"
                     /translation="MDASAPPARKSLGQHFLIDPNIVRKIVALADVQPGEPVFEIGPG
                     RGILTEALCRVASRVIAVEVDPRLHTYLSGRQSDFPNLELISGDVLTYPIDDLPCGTV
                     VVANLPYNISTPILFRLLEVGPRLSRMVLMLQAEVAARLMAKPGSREYGLLSVMAQSA
                     AEIVEGFRVSAQCFRPRPEVASAVVLLRHRATALVSSEQQANFHRIVKAAFAHRRKTL
                     PNSLRDEGYVVDRVVDALHALNLPPTVRAETLSVEQFIALTSRVA"
     gene            complement(2141..2458)
                     /locus_tag="VI359_01820"
     CDS             complement(2141..2458)
                     /locus_tag="VI359_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973019.1"
                     /translation="MSEKLQLGLAAIQQQMKELDEIAKKTTEDLNTVAGTERVTKWKA
                     RTAAMLSQALGEHEGKAFARQQPGPSFTNDLFEEFTDIVDYYRSALRTMSKKLESSIP
                     KTS"
     gene            complement(2455..3513)
                     /gene="pdxA"
                     /locus_tag="VI359_01825"
     CDS             complement(2455..3513)
                     /gene="pdxA"
                     /locus_tag="VI359_01825"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY6973020.1"
                     /translation="MKRPPATQSHKLPLLGITMGDPAGIGPEVIAKALAGTALRRLCR
                     PLVIGSVPVMERTIKSLKLKLQVAPVAGHDATPFRPGKVAILDPLDSPLGRFSVGIAA
                     PETGAASVAFIKKAVRLAELGCIDGIVTAPINKEAINMAGCHYPGHTELLADLTHSPE
                     SGMMIVGGPLKIMFVTTHVAIKELPAVLTQARIEKAIRLAHRALRELFHIQKPRIGVA
                     ALNPHAGEHGLFGDEEATAIRPAARAAQAQGILASDPLPADTLFGKAARGDYDGVVAM
                     YHDQGLIPLKLVAFGTCVNLTVGLPIIRSSVDHGTAYDIAGKGVADHGSLLEAVKLAA
                     KLARGRGHRRTVSLEGLA"
     gene            complement(3516..>3926)
                     /locus_tag="VI359_01830"
     CDS             complement(3516..>3926)
                     /locus_tag="VI359_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973021.1"
                     /translation="GLMGLILGAHLFITAAETIAGTLAMSPLILSLLVAPLATELPEM
                     SNSFLWLYRKKDRLAVGNVTGAMVFQGTFPVSVGLLGTEWALRPTVVVTMVLAVIAAS
                     SCLLQAAWGGHWRPWLLSGSALLYIGYAIYLYGW"
BASE COUNT          841 a         1226 c         1095 g          764 t
ORIGIN      
        1 aatatcctta cccagcgcca gcgtcagttt cgatcgtgct ctggtaaaca cctcgctggt
       61 caccacatct ttcaaggaga cggtttctcg atagggattc ggcacctcga tgcccaccac
      121 ggatttgccc gggagcggcg caacgatcct caggctgatc gccttcatcg ccagagcgag
      181 gtcatcggcc agattgacaa tcctggcgac tttggtccca ggagccggct caaattcata
      241 catcgttacg accgggccgg gccggacatc cgtgactttt ccctcgatgc cgaagctgag
      301 gagtgcccgt gcgaggatct ccgattgcgc tttcagctct tcctccgtca tccgagccag
      361 tgccccggac ggctcgctga gcaactcggc cggatccggc aacacataat catcagcttc
      421 gtcctgggcg gctgatatct gaggttccgg ctcggtctcg agtggctccg gcgcctcttc
      481 caccggtttc ggaagcggct gagcaacgct cgagtccaca accggggacg gttctgtcgc
      541 cggcaagact tcttcaatga ccgatcggat cgatttagct gctttcgcct tggctttctt
      601 caccggcgcc tcgagttccg gcgcgggacc tcgctccggc atcaaggcag agatgcggct
      661 gaccgcacgg ctccaccatt cagaaagacg aagtgccagt tgagccagcg acaccggtgt
      721 cgtgtacagc atcgacacca agaaccccgc gataatcagg atatgcgccc cggtactagc
      781 aaagtaggtt cgtaagcctt cggccaagac ctgtccgcag attcccccgg cgatgccgcg
      841 atagacaaac ccgctcgaca cggtcggcac tgcggttact tcgagatgga gaaacccgct
      901 caggaagagc agtacgccca aagaggccgc cccattgcgg atacggatcg agagggtact
      961 ctgcgtgaaa catcgcaccc ctatcagacc gagaaggaga gggaaaagat acgccgctcc
     1021 cccaatcaga aaaaacaggc tggaggcaaa cagcgctccg acagacccga tgaggttcct
     1081 gggcggcggc gccagcgacc cggacaagac tttcccttca cctgggacaa aggaaaccag
     1141 gctcaggagc gtcaacaggc tcaacgcgat cagtacaacc ccaatgactt cgcgcttgag
     1201 atgagagggc ggagatgagg cccgccgggc atcgccccgc ttggccgtgg tggaggcacc
     1261 cataggcgga tgctagcata gggaacgagt catttcaaac gaacgacccg ctgaattcct
     1321 ctcccacggc gggttcttct cacgcgactc gagaggtcaa tgcaatgaat tgttcaacgg
     1381 acagagtttc tgccctgacg gtcggcggaa ggttgagcgc atggagggca tccacgactc
     1441 gatcgaccac atatccctcg tcgcgcagcg aattgggaag cgtcttccgg cgatgagcga
     1501 aggccgcctt gacgatccga tggaagttgg cctgctgctc gctcgacacg agcgcggtgg
     1561 cacgatggcg cagcaatacg accgccgacg ccacctccgg cctgggacga aaacactgag
     1621 ccgagacgcg aaatccctcg acgatctccg ctgctgattg cgccatgaca gacagcagac
     1681 catattcccg actgcctggc ttcgccatca gtctggccgc aacttccgcc tgaagcatca
     1741 gtaccattcg actgaggcgc ggcccgacct ccagcaaccg aaacaggatc ggcgtcgaga
     1801 tgttataggg caagttcgcc accaccacgg ttccgcaagg cagatcatcg atcgggtagg
     1861 tcagcacatc tcccgaaatg agctcaagat tcgggaaatc agattgccgt cccgacaggt
     1921 acgtatgtaa ccgtggatcg acttcgactg caatgacacg actcgcgacc cggcagagag
     1981 cttcggtcag aatcccacgg cccggcccga tctcaaacac cggttcgcca ggctgcacat
     2041 cggccagcgc gacgatcttg cgcacgatgt tcggatcgat gaggaaatgc tggcccaagg
     2101 acttgcgcgc gggaggtgcc gaggcatcca ttaaagtgga tcaactggtt ttggggatcg
     2161 acgactcgag cttcttcgac atcgtccgga gggccgagcg gtaatagtcc acgatgtccg
     2221 taaattcctc gaacagatcg ttcgtaaatg acggacctgg ctgctgcctc gcgaaggcct
     2281 tcccttcatg ttctccaagg gcctgactca gcatagcggc cgtccgagct ttccatttcg
     2341 tcacccgttc ggttcctgcg acggtattga gatcttccgt cgttttcttg gcgatctcgt
     2401 ccaactcctt catttgttgc tgaatggcgg caagcccgag ttgaagcttt tcactcatgc
     2461 taatccttcc agagaaacag tccgcctgtg cccacggccc cttgcgagct tggccgcgag
     2521 cttgaccgct tcaagcaagc tgccgtggtc ggcaactccc ttaccggcga tgtcgtaggc
     2581 agtcccgtga tcaaccgagg atcgaatgat cgggagtccc acggtcaagt tgacgcaggt
     2641 gccgaacgct acaagcttga gtgggatcaa tccctgatca tgatacatcg caaccacacc
     2701 atcatagtcg ccacgcgccg ccttgccaaa tagcgtgtca gccggtaacg ggtcacttgc
     2761 caggatgccc tgtgcctggg ctgcgcgcgc agccggtcga atggccgtcg cttcttcatc
     2821 gccgaacaga ccgtgttccc ctgcgtgagg gttcaacgcc gccactccga ttctcggctt
     2881 ctggatgtgg aacaactctc gcagagccct gtgcgcgagc cgaatggcct tttcgatgcg
     2941 cgcctgtgtt aataccgccg gtaactcttt gatcgccacg tgagtcgtca cgaacataat
     3001 tttgagcggc ccaccgacga tcatcatgcc gctttccgga gaatgggtca ggtccgccaa
     3061 taattccgta tggccggggt aatggcaccc agccatgttg atggcttcct tgttaatcgg
     3121 ggcagtgacg atgccgtcga tgcaccctag ctctgccaac cggactgctt tcttgatgaa
     3181 cgctacggaa gcggcaccgg tctccggtgc ggcgattccc acgctgaatc gtccgagcgg
     3241 agagtcgagg ggatcgagaa tcgccacctt gccgggtcga aatggtgtgg catcgtggcc
     3301 ggcaaccggt gcaacctgga gcttcagctt caatgacttg atcgttcgct ccataaccgg
     3361 aaccgacccg atgacgagtg gacggcacag ccgtcgcaat gccgtccctg ccagcgcctt
     3421 ggcaatcacc tcggggccaa tccccgccgg atcgcccatt gtgatcccca aaagcggcaa
     3481 cttgtgtgat tgtgtggccg gcggcctctt cattctcacc aaccgtacag atagatggcg
     3541 taccctatat aaaggagagc gcttccactg agcaaccagg gtcgccagtg gcctccccat
     3601 gctgcttgga gcagacagga actcgcagca atgaccgcga gcaccatcgt gaccaccacc
     3661 gtcggtctca atgcccattc cgttccgagc agccccaccg aaactgggaa tgtcccttga
     3721 aacaccatcg caccggtgac gttgccgacc gccaatcgat ccttcttcct atagagccat
     3781 aaaaaactat tggacatctc gggcaattcc gtcgcgagcg gtgcgactaa cagagacaag
     3841 atcagcggtg acattgccag cgtacccgca atcgtctccg cggcagtgat gaaaagatgt
     3901 gcgccgagaa tgagccccat cagccc
//
LOCUS       DATDNW010000069         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_758182, whole genome shotgun sequence.
ACCESSION   DATDNW010000069 DATDNW010000000
VERSION     DATDNW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758182"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VI359_01835"
     CDS             <1..193
                     /locus_tag="VI359_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973022.1"
                     /translation="DGLYTRRDPYPNIQILRVDVGERAYQSIKGSGYYQRWTESNLRD
                     VSASPISWRRNQCAAPSLS"
     gene            196..561
                     /locus_tag="VI359_01840"
     CDS             196..561
                     /locus_tag="VI359_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY6973023.1"
                     /translation="MIPIEGKGRGVVVTQPIAAGTVIEIAPVVPYRTECGRDEMPDRS
                     LPPELENLPHDWDEECACLVFGAMQFANHSDAPNARFTRNVKALSMTFTALRDIKAGE
                     EITIDYGIPVWWGCTQTNP"
     gene            612..1622
                     /locus_tag="VI359_01845"
     CDS             612..1622
                     /locus_tag="VI359_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY6973024.1"
                     /translation="MSTSAAIRETLVSQLVACRRMLYANSWMAGIMRAVGLRSYLEDI
                     SLHEHMLADSVRLHAYHEAIRRYVMPQDDVVDVGTGTGVLAFFAAAKSPRRVYALDHS
                     KRMLELAAAAAAANGIGCSNVIFDASTSRRFRPAEPVDVIVHDQMGVALFDEGMVETI
                     LDLRDRCMKPGGRILPAKFEFYLEPVQLVSRERIPLLQEQCVYGLAFPRPAIAPTSDY
                     YFREIYPEDVEYLLCEPAASFAFDLSTLSREQLPSQFSTKKPVKRDGQRDGICIYFKA
                     IFDNDISFSTGPDSAKTHWPMLFYRTPARIYRVGETLSLKVDTPDLSEHHGWSWQIDS
                     PT"
     gene            2620..>4515
                     /locus_tag="VI359_01850"
     CDS             2620..>4515
                     /locus_tag="VI359_01850"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEY6973025.1"
                     /translation="MVQHTSEARSKEPLAPWTPDLVQDAWSYWFDAFQRGVLFLDVMR
                     QRSQRREEHIAKLAPHVLKFRTELVMDGRTLPRPVNYVLARITPPSDVEIDENKRPFV
                     IVDPRAGHGPGIGGFKAQSEIGVAFNAGHPCYFIGFLPDPVSGQTIEDIAEAEAAFLE
                     RVIALHPKADGKPAVIGNCQAGWAVMMVAAKRPELFGPIIVAGSPLSYWAGVRGENPM
                     RYTGGLLGGSWLTALVGDLGNGKFDGAWLVSNFENLNPANTYWSKHYNLYSKVDSEAP
                     RYLEFEQWWGGHVLLNAQEMQSIADRLFIGNKLATAEIVSSDGTTIDLRNIRSPIVVF
                     CSKADNITPPPQALDWILDLYESVDDIRAHGQTIIYAIHPTAGHLGIFVSGAVAKKEH
                     DEFASNIDLIDVLPPGLYEAVMTPKQTADETADLVSGDYLVRFETRTLDDIRALGGNS
                     PEDERNFATAARISEINLGLYRTFVQPWIRPMATPGFADWMQRMHPLRLQYEAFSRAN
                     PFMGGVPSAAESVREHRQPVPANNVFWQAQQWTSNWIETSLNIYRDVRDQWFETLFHG
                     VYGSPAVQALAGLRASETSPRRRPGVDSVYRTFVARRVEELIWGIGEGGAREAAIRAA
                     LYIRLPEG"
BASE COUNT          933 a         1310 c         1309 g          963 t
ORIGIN      
        1 cgacggcctg tacacgcgac gggacccata ccccaacatt cagatacttc gggtggacgt
       61 cggagaacgg gcctaccaga gcatcaaggg atccggttac taccagcgtt ggactgagag
      121 caatctccgg gatgtcagcg ctagccctat atcctggcga cgaaaccaat gtgcggcgcc
      181 aagcctgagc tgaacgtgat tcccatcgag ggaaaggggc gcggggttgt cgtgactcag
      241 cctattgctg ccggcacggt tatcgagatc gcgccggtcg taccctatcg aacggagtgc
      301 ggtagagatg agatgcctga tcgctcgctt ccaccggagc tggagaactt acctcacgat
      361 tgggatgagg aatgtgcgtg cctggtattt ggtgcgatgc agttcgccaa ccattcggat
      421 gcgcccaatg cccggtttac ccgcaacgtc aaggcgctct caatgacctt cacagccctt
      481 cgcgatatca aggctggcga agagatcaca atcgattatg gaattcctgt ttggtggggg
      541 tgcactcaga ccaatccatg atccctgctt aaactgtcct cgctcgtgcc accgagggga
      601 ggaggccgct catgagcacc agtgccgcca tccgagagac tcttgtctct caactcgttg
      661 cttgccgccg catgctctac gcaaattcct ggatggctgg gatcatgcgg gccgtcgggc
      721 ttcgtagcta tttggaggat atctctctgc acgagcatat gctcgccgac tcggtaaggc
      781 ttcatgctta ccatgaggcg atcaggcgtt acgtaatgcc gcaagacgac gttgttgacg
      841 tcggcacggg taccggcgtt ctcgccttct ttgccgcagc gaaaagtcct cgccgagttt
      901 acgcgctcga tcattccaag aggatgttgg agcttgccgc ggctgcggct gccgccaacg
      961 ggattggctg cagcaacgtg atcttcgacg cttcaacgag ccgcaggttc cggcccgcag
     1021 aacctgttga cgttatcgtc cacgaccaga tgggagtcgc actattcgat gagggaatgg
     1081 tggaaaccat tctcgacctc cgcgaccgct gcatgaaacc cggcggacgc atcctgcctg
     1141 cgaagttcga gttttatctt gagccggttc agctcgtgag cagggagcga attccattgc
     1201 ttcaagagca atgcgtctac ggcttggcct tcccccgacc agcgatcgcg ccgacgagcg
     1261 attactactt ccgtgaaatc taccccgaag atgtcgagta tttgctttgc gaaccggcag
     1321 cgtcgttcgc cttcgatcta tctaccttaa gccgtgaaca gcttccctcg caattctcta
     1381 cgaaaaaacc tgtcaaacga gacggccaac gcgatggcat ttgcatctat ttcaaggcca
     1441 ttttcgataa tgatatttcg ttctcaacgg gtcccgactc ggcaaagact cactggccaa
     1501 tgcttttcta tcgaacgcct gcgcgtatct atcgagtcgg cgaaacatta agcctgaaag
     1561 tcgacacgcc cgatctgtca gaacatcatg gctggtcctg gcagattgac tctccgacct
     1621 agtctgccag gacggtgaaa gcgatggctg ctatgggtca tatgcggaca gtctggaggg
     1681 gcgatccgta cgtccgctaa gtgccaatag cagatgtcaa acccggggac ggggagcctt
     1741 caaactaccc agcgcgcagt cgtcttaccg aagtcgatcg cctaagtatg agaagttccg
     1801 atcttgccac gcgcatttcc tgtcgtggcc acggaatcaa atgtcttcga catcggtggc
     1861 gaggatgcca gatgcgttaa atcggttaca agcttctgga gaaccacttg aggtaacgag
     1921 aactctccag cgcaaacgag cgaacgtgcg ccgagcccgc tcgaagagtc cgctgtgtgc
     1981 cacaagtgga cttcaagcag ccgcagtgag tggatttttg gagaatgatg accccttgct
     2041 ggccccaaat tctacgtttg gcggtctggt taggtcggaa agccgaaatg tcccattagg
     2101 caaaaagcga atgcccggtt gaaatcagtg ccacctcatc cgaaataggt tttcggggct
     2161 gggtcggaga cctggccatg agggcgttct tgaaattctc atttcgctgt taggcgcgat
     2221 gcttttccct gtttgcaaca aaaattccct gtttcgattg caggggattt ttccaaagag
     2281 atctcgcgtt tcaacggttt tatgcaagcg gggaggggcg tttcaacgga aattccctgt
     2341 attttccctt caccctccta aggagagtga tcgtgttccg cgattacgtc cgccgactca
     2401 gtgaaagctg cttttggatc agcaagtggg tggtggccac ggcggttctc gttgcgatgt
     2461 cggcatcacg ctcaaatggg cgtaagaggg agaataaaat cggccctcaa agggccgtag
     2521 atcattggta tgctcaggcg aaggtgagga gtcgatgtcc gtcaaggcct ccgtcacctc
     2581 gcctccataa agggtgctgg tacagtggag gaggcggaca tggttcaaca cacatccgaa
     2641 gctcgctcta aggagccgct agcgccgtgg acaccggacc ttgtccagga cgcatggtca
     2701 tactggtttg acgcttttca gcgcggcgtt ctcttcctcg atgtcatgcg gcagcgtagc
     2761 cagcgccgcg aggagcatat cgcgaagctc gcgccccatg tgctcaagtt ccggaccgag
     2821 ctcgtgatgg acggacggac tttgcctcgt cctgtgaact atgtcctggc gcgcatcaca
     2881 cctccgtccg acgtcgagat cgacgagaac aaaaggccct tcgtgatcgt tgatccccgc
     2941 gcaggccatg gtcccggcat cggaggtttt aaagcgcaga gcgagatcgg cgtggcattc
     3001 aacgccgggc acccttgcta cttcatcggc ttcctgcccg acccggtttc cggccagacc
     3061 atagaggaca ttgccgaggc tgaagccgcc tttctcgagc gcgtgatcgc gctgcatccg
     3121 aaggcggatg gcaagccggc ggtgatcggc aactgccaag ccggatgggc cgtgatgatg
     3181 gtggctgcca aaaggcctga gttgtttggc ccgatcatcg tcgctggatc cccactctcc
     3241 tattgggccg gcgtgcgcgg cgagaaccct atgcgctaca ctggcggcct gctcggcggc
     3301 agctggttga ccgccctcgt gggagacctc ggcaacggca aattcgacgg ggcgtggctc
     3361 gtcagcaact tcgagaatct caatccggcc aacacttact ggagcaagca ctacaatctt
     3421 tattcaaagg tcgacagcga ggctccgcgt tatctcgagt tcgagcaatg gtggggcggc
     3481 cacgtccttc tcaatgccca ggagatgcaa tccatcgcgg atcggctctt catcggcaac
     3541 aagcttgcca ccgccgagat cgtttcatct gacgggacga ctatcgacct gcgcaacatc
     3601 aggtcgccga tcgtcgtgtt ctgctcgaag gccgacaaca tcactccgcc gccgcaggct
     3661 ctcgattgga tcctcgatct ctatgagagc gtagatgaca ttcgcgcgca cgggcagacg
     3721 ataatctatg ccatccaccc aacggccgga catctcggca tcttcgtttc cggcgcggta
     3781 gcgaagaagg agcacgacga attcgcgagc aacatcgatc tcatcgacgt gctcccgcca
     3841 ggtctttatg aggcggtgat gacgccgaag cagacggccg acgagactgc cgatctcgtc
     3901 agtggcgact acctcgtgcg cttcgagacg cgcaccctcg acgacatcag ggctctcggt
     3961 ggcaacagcc cggaagacga gcgcaacttt gccaccgcag cgcgcatttc cgagattaat
     4021 ctcggactgt atcgcacgtt tgttcagccc tggattcggc cgatggcgac cccgggtttc
     4081 gccgattgga tgcagcgcat gcacccactc cgcctccaat acgaggcatt ctcccgcgcc
     4141 aacccgttca tgggcggggt gccgtcagcc gcagaaagcg tgcgggagca ccgtcagccg
     4201 gtccccgcga acaatgtgtt ctggcaggcg cagcaatgga cctcaaactg gatcgaaacg
     4261 tcattgaaca tctaccgtga cgtgcgcgac cagtggttcg agaccctatt ccacggcgtg
     4321 tatggctcgc cggcagttca ggcgcttgcc ggtctgaggg cctcggagac gagcccacgc
     4381 cggcgaccgg gcgttgacag cgtctatcgc acttttgtcg cccgtcgcgt cgaagagctg
     4441 atctggggca ttggtgaggg tggagcgcgc gaggcggcga tcagggctgc actctatatt
     4501 cgcttgcccg aggga
//
LOCUS       DATDNW010000070         4833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_419644, whole genome shotgun sequence.
ACCESSION   DATDNW010000070 DATDNW010000000
VERSION     DATDNW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419644"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VI359_01855"
     CDS             complement(<1..1113)
                     /locus_tag="VI359_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY6973026.1"
                     /translation="MSDFYQNGVVTVLHRLGSPNVDRLEDELERCGRMHPIALVLPSL
                     YAELERPALKKIVETLKTVTYLHEIVISLDRASALEFRLAKQYFADLPQRVRLVWNDG
                     TRIQTLLNLLVSQNIDIGLPGKGRGCWLAFGYVLARNHSQVIALHDCDITSYHREYLA
                     RLCYPVANPNLGYEFCKGYYSRVTDRLHGRVTRLFITPLIRSLQQLLGPHALLTFLDS
                     FRYPLAGEFAMVRDLAWINRIPGDWGLEIGVLAEVYRNCALRRICQADIADAYDHKHQ
                     SLSSQDPDAGLLKMCVDITKSLFRNLASEGVVLSDGILKTLRATYLQAAQDAISRYEN
                     DAAINSLTFDRHEERTAVEVFLKGMKLATEGFLEDPL"
     gene            complement(1345..3000)
                     /locus_tag="VI359_01860"
     CDS             complement(1345..3000)
                     /locus_tag="VI359_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6973027.1"
                     /translation="MIRKPTIGSTVIDRLHKLGVRHIFGIPGDYVLSLYKLLETSPIR
                     HIGTTREDCAGFAADAYARINGIGAVCVTYCVGGLNTVNAIACAYAERSPVVLLTGSP
                     GLSERTRTPYLHHMVRDFSTQREVFEKMTVAAVSLDDPLTAEREMDRAFAALLRYRRP
                     IYIEIPRDMVHAPLSSPSRPMCIEDEPSDPAALAEAIGEVRAMLTSASRPAILAGAEI
                     GRFGLQDDLGRLVERLNIPIASTLLGKSVIREDHPLYVGVYGGLIARDDVQQFINQSD
                     CLLILGSILSDVEDLDVRSPLLMEGRTIHATADRVAIKHHRYDAIRFEDFVRALATQS
                     LPSFPSRSLPPVIPIEAGTSTPDSSITLNGLFHHLDSVLDEKTLVIADVGESLFAAAD
                     LHVHRRFEFLSPAYYTSMGFAVPAAVGASFADPSLRPIVLVGDGAFQMTGTELSTAVR
                     YGQTPIIIVLNNRGYSTEREILEGPFNDLHEWQYERICEVVGGGVGSRIRTQGEFEQK
                     LAAALADSSQLHIFNVLLNPADRSAGMKRLAHRLAKRLSTDRP"
     gene            3111..4301
                     /locus_tag="VI359_01865"
     CDS             3111..4301
                     /locus_tag="VI359_01865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY6973028.1"
                     /translation="MAREDAPTATIQLSRTRIEQDSGHLWVYAGHIDEVRGDPTAGDV
                     VDIMAPNGKFVARGLYNPHSKIRVRLLTFDAEPITEQFWRGRVAQAIRLRQRIVSDTN
                     AYRLLYGEADQLPGLIVDHYNDVLVMQTLSYGMDRRKALLADLLCQEAGVGRIYLRND
                     AKSRSLEGLPIEKGFLRGGGATRVEIQEARASFLIDIERGQKTGWFCDQRENRLAAAR
                     LTAGAEVLEVFCHTGAFGTHAALGGARSVEGLDVSEDALVLAREHAILNKVGDRCIYR
                     AADAFDEMRSLERAGRRYDVVMLDPPAFARSKQAVPRALAGYKDVNLLGMRLTKPEGF
                     MVTSSCSHHVSDQDFWIAIRHAARDARRQVRLLEQRGQASDHPVLAAMPETRYLKCFL
                     LQIL"
     gene            complement(4324..>4833)
                     /locus_tag="VI359_01870"
     CDS             complement(4324..>4833)
                     /locus_tag="VI359_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY6973029.1"
                     /translation="VGRRVAEGGMIQYHRPMAIGELIHAWRVSRGRSLEDVATDAQLS
                     PIVLEEIESECVDPSASTIESLAAALRIPVAWLFAHPTTFEVLFERLEEEPTTTPDGP
                     DPITERILAGSRMDRSLYVLLTALLQSGEPKLLRAAEASLRSLVKQSKQATVPWQHRP
                     SGHFEPPSD"
BASE COUNT         1145 a         1275 c         1440 g          973 t
ORIGIN      
        1 cagtggatct tcgagaaacc cctccgtcgc gagtttcatc cctttcagaa acacctcgac
       61 ggccgtccgt tcctcatgac ggtcgaacgt gaggctgttg atggccgcat cgttctcata
      121 tcggctgatg gcgtcttgag cagcttggag ataggtcgcc cgcaaggttt tcaggatgcc
      181 gtccgacagc accacgcctt cgctcgctaa attcctaaag agggacttcg tgatgtcgac
      241 gcacattttt aacaacccgg catctggatc ctgactcgag agagattggt gtttatggtc
      301 ataggcgtct gcaatatcgg cctgacagat acgccgcaac gcgcagttgc ggtacacctc
      361 cgccagcacg ccgatctcca acccccaatc cccggggatg cggttgatcc atgccaggtc
      421 gcggaccatg gcgaattctc cagcgagagg ataacgaaag ctgtcgagaa aggtcaggag
      481 tgcgtggggt cccaggagct gttggaggct tcgaatcagc ggggtgataa agagccgtgt
      541 gactcgacca tgcaaacggt cggtgactcg gctatagtag cccttacaaa attcataacc
      601 gaggttcggg tttgcaacag gataacagag acgtgccaga tactcgcggt gatagctggt
      661 aatatcgcag tcgtgcaggg cgatgacctg actatgattg cgcgccagca catagccgaa
      721 cgccagccaa cagccacgtc ctttgcccgg caatccaata tcgatattct gtgagacgag
      781 caggttgagg agggtctgga tgcgggtgcc gtcattccag accagccgga ctcgctgggg
      841 aagatcagcg aagtattgtt tggccaggcg aaactctaac gctgacgccc ggtcgagtga
      901 aattacgatt tcatgaaggt atgtgacggt tttaagggtt tcgacaatct ttttgagagc
      961 cggtcgttcc agttcagcat agagagacgg cagcaccaag gcaatcgggt gcatacgtcc
     1021 gcagcgctct aattcgtcct ccaaccggtc gacgttgggg ctgccgagcc ggtggagaac
     1081 ggtcaccacg ccattttggt aaaagtccga catgcggaac tccagtcaga actcgaaggg
     1141 tcacccttta gaggacgtgc acagagaagg ataagaagcg gttacgacaa ccagtggata
     1201 aatacggatt tagaatacct gaattgcaca gggtgtgcca tatgaaattc aatgaaaagc
     1261 caggccccgg cagtttcccg tttcagagcc ggggtattgc ctagtggcaa aagccttcac
     1321 ttatacagag gaatcagata cacgtcatgg cctgtcggta gacaggcgct tcgcgagacg
     1381 gtgggccaat cgcttcatgc cggcagaacg atcggcagga ttgaggagga cgttaaagat
     1441 gtgtaattga ctggaatcag ccaaagccgc agcaagtttt tgttcgaatt ccccctgcgt
     1501 ccggatgcgt gagccgactc cgccacccac gacctcgcag attcgttcat attgccactc
     1561 atgcaggtcg ttgaacggac cttcgagaat ttcccgctcg gtcgaatacc cacgattgtt
     1621 caaaacgatg atgattggcg tttgaccata ccggaccgcc gtcgagagtt cagtgcccgt
     1681 catttgaaac gcgccatcgc cgaccaagac aatgggccgg agtgagggat cggcgaatga
     1741 ggcacccacg gcagccggga cggcaaagcc cattgaggtg taatacgcgg gcgagagaaa
     1801 ttcaaatcgc cgatggacat ggagatcggc tgcggcaaat aatgattctc cgacgtcggc
     1861 aatcacgagc gttttctcgt ccaaaacact gtcaaggtga tgaaagagtc cgttcaacgt
     1921 aatggaggag tcgggcgtgc tcgttccggc ctctatagga atcaccggcg ggagagaacg
     1981 tgagggaaaa gacggaagtg attgagtcgc caacgctcgg acaaagtctt cgaaccgaat
     2041 ggcatcatag cggtgatgtt tgatggcgac acggtccgcc gttgcatgaa tggtccgacc
     2101 ttccatcagc aacggagaac gaacgtcgag gtcttccaca tcggagagaa tcgagccgag
     2161 aatcagtaaa caatccgatt gattgataaa ttgttggaca tcatcgcgcg caatcagtcc
     2221 cccatacaca ccgacgtaga gcgggtggtc ttcacgaatc accgatttgc ccagcaacgt
     2281 ggaggcaatg ggaatattca ggcgctcgac aagccggccc aggtcgtctt gcagtccgaa
     2341 acgcccgatt tcggcaccgg ccaggatggc gggacggctg gcggaggtca gcatggcccg
     2401 aacttctccg atggcctcgg cgagggctgc cggatcgctc ggttcatctt cgatacacat
     2461 aggacgagaa ggagaggaga gaggggcatg gaccatgtcg cgcgggattt cgatatagat
     2521 tggtcgacgg tatcgaagca aagcggcaaa ggcacgatcc atttcacgtt ccgccgtgag
     2581 aggatcgtcg agcgaaaccg cggcgaccgt catcttttcg aagacctcgc gctgggttga
     2641 aaagtcgcgc accatatgat gcaagtaggg cgtgcgggtt cgctctgaaa gtcccggaga
     2701 gccggtcaac aggacgacgg gagatcgttc ggcatatgca caggcgatgg cgttgaccgt
     2761 attgaggccg ccgacgcaat aggtgacaca gaccgcgccg ataccgttga ttcgcgcata
     2821 ggcgtctgcg gcaaaaccgg cgcagtcttc acgcgtcgtt ccaatgtgac gaatcggcga
     2881 ggtttcgaga agtttgtaga ggctgagcac atagtcgccg ggaatgccaa agatatggcg
     2941 aaccccaagc ttatgcagtc gatcaatgac cgtggaaccg atggttggct ttcgtatcat
     3001 cgcggcctct tcctaaatcc agcaaaccac agctggcgac agccgtcaag caaaggaaga
     3061 accatagagg gacttgacct tcctctcgtg caattggata agtacctgtg atggcccgag
     3121 aagacgcacc taccgcgacg atacagctct cgcgcaccag aatagaacag gattccggac
     3181 atctctgggt ctacgctggc catatcgatg aagtcagggg agacccgacg gcgggtgatg
     3241 tggtcgacat catggcaccg aacgggaagt ttgttgcacg aggcctctac aatccacact
     3301 ccaagattcg agtgcgcctg ctcaccttcg acgcggaacc catcacagaa caattttggc
     3361 gggggagggt ggcgcaagcg attcgtctgc ggcagcgaat tgtgtcggac acgaacgcgt
     3421 atcgcctcct gtatggggag gcggatcaat tacccgggtt gatcgtggat cattacaatg
     3481 atgtattggt tatgcaaacc ttgtcttatg ggatggatcg ccgaaaggct ctactagccg
     3541 acctgctctg tcaagaagcg ggagtcggtc gaatctacct gcggaacgat gcgaagagtc
     3601 gaagtttgga agggttgccg atagaaaaag gattcctccg tggaggagga gccacacgag
     3661 ttgagataca agaagcgcgg gcgtcttttc tgatcgacat tgaacgaggc cagaaaaccg
     3721 gctggttttg cgatcaacgg gaaaatcgac tggcagcagc gcgactcacg gccggagccg
     3781 aggtactgga agtgttctgc cacacaggcg cctttggcac gcacgcagca ctgggcggtg
     3841 ccagatcggt tgaagggctc gacgtcagtg aagacgcgct cgtgttggct cgagagcatg
     3901 cgatcttgaa caaggtaggc gatcgatgca tctatcgagc agctgacgcg tttgacgaga
     3961 tgcgaagctt ggagcgagcc ggtcggcgct atgacgtggt catgctcgat ccgcccgcct
     4021 ttgcccgaag caaacaagca gtcccacggg ccttggctgg atacaaagat gtcaatttgc
     4081 ttgggatgcg gctcacaaag cccgaaggat tcatggtgac gagttcctgc tctcaccatg
     4141 tcagcgacca agatttctgg atcgccatcc gccacgcggc gagggatgcg aggcggcaag
     4201 tccgtctgct tgaacagcgt gggcaagcca gtgaccatcc ggttctggcc gcgatgccgg
     4261 agacacgcta cttgaagtgt ttcctgctgc aaatactcta aatgcgcagt ggaacgtcgg
     4321 cagttagtcg ctaggtggtt cgaagtggcc ggagggacga tgctgccagg gcaccgtggc
     4381 ttgcttggat tgcttcacca ggctccgcaa gctcgcctcc gcggctctca agagcttcgg
     4441 ttcaccgctt tgcaggaggg cggtgaggag cacatagagc gagcggtcca tgcgagaacc
     4501 agcaaggatc cgttcggtta tgggatccgg cccgtccgga gtcgtcgtgg gttcctcctc
     4561 cagtcgctcg aataacacct caaaggtggt cggatgggca aagagccagg ccaccggaat
     4621 tcttaaggca gctgccagcg attcgatggt cgatgccgat ggatcgacac actccgattc
     4681 aatttcctcc aagacgattg gcgaaagttg cgcgtcagtt gccacgtcct caagtgaccg
     4741 gccgcgcgaa actctccagg cgtgaatcaa ttccccgatc gccatgggcc gatgatactg
     4801 aatcatccct ccctccgcaa ctcgtctccc aac
//
LOCUS       DATDNW010000071         5726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_963313, whole genome shotgun sequence.
ACCESSION   DATDNW010000071 DATDNW010000000
VERSION     DATDNW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963313"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VI359_01875"
     CDS             <1..719
                     /locus_tag="VI359_01875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY6973030.1"
                     /translation="VGFHIEHKNVINLVTDADCRAEQRIVDVIQKAVPSHRILAEERG
                     LTRQLPSPYQWVIDPLDGTTNFAHGFPAYCVSIGVEYEGRGLIGVVFDPTRDELYTAQ
                     RGKGAFLNDTPISVSSVDHLDRALLVTGFAYDIRETPNNNLDHFVRFALKAQGLRRTG
                     TAALDLCYVATGRFDGFWEVALNPWDMAAGIVILEEAGGKVTNFKGKTHSIYEKELVA
                     SNGRIHEAMLNVLQQNHPLA"
     gene            869..1153
                     /locus_tag="VI359_01880"
     CDS             869..1153
                     /locus_tag="VI359_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973031.1"
                     /translation="MASSTNPSGKSNGHPSQSAAAESTTWDVELLRVLVSRKGTRVDA
                     EWAIHPQLKQDLLPTEWQEVTDLMTKVTGIVGTRFSQVLSQADPEPPSNA"
     gene            1224..1556
                     /locus_tag="VI359_01885"
     CDS             1224..1556
                     /locus_tag="VI359_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEY6973032.1"
                     /translation="METYYHPHDLGKFADMGKGNQALWEKFMSYYSSVFAEGALTERE
                     KALIALAVAHTVQCPYCIDAYTQASLEKGSNIEEMTEAVHVACAIRGGASLVHGVQMR
                     NVAEKLSM"
     gene            complement(1809..3668)
                     /locus_tag="VI359_01890"
     CDS             complement(1809..3668)
                     /locus_tag="VI359_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HEY6973033.1"
                     /translation="MNDKAQVALVNMPFSFSKYPSIQLGTLSALLKSKGIPVDCHHLN
                     VRFAHKIGVPLYELICEKRALFGEWLFSHLLFRDNPKHAEYPRMFKPVFEQITQESGH
                     PMSFFEDMAGRTAPQFLTWAMTAIDWGQYKLVGFTSTFDQNVASLTMAKLIKDLYPHV
                     TIVFGGANFDGEMGLEHFRAFPWIDHVVVGEGEDTFPALVTYVLSGKTGVPPPGVTYR
                     QSGQIRYEPNQSLFSDFTQTGPPDYDDYYRLLAELGKEAAQGLDRILLYEGSRGCWWG
                     EKHHCTFCGLNAQSMRFRAKSSEQVAREMAYLSSRYDTARFRLVDNIIDMKYIENLFG
                     KFAADHCDLDVFIETKSNLHKNQIRTLAAGGVKCMQPGLESLSLNQLRAMDKGVTPMQ
                     NIICLKWSFYYHVEVSWNILLGFPGETTEDYRRQIDLIPALFHLQPPEATGKFWLERF
                     SPYYKRPHDYGVRITGPGLAYEYVYDGRQVDLKKIAYDFEYELDNWPVDPHVYQELVD
                     VVGAWKRLHASADRPFLYYSKAMGYITVYDGRNPKAPMRHRFDQPEAGIIEACNEAAK
                     SEDQIHSILAERPDCSCSASRLADALASLTARRILYEERGKYFTLAIPENPYL"
     gene            complement(3711..4061)
                     /locus_tag="VI359_01895"
     CDS             complement(3711..4061)
                     /locus_tag="VI359_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY6973034.1"
                     /translation="MGLADNKCVPCRGGVPPLPPERSQALLKELGRGWSLNGAGHLER
                     LFTFKDFAQALAYVNKVGAIAESEGHHPDLYLAWGKCKVEIWTHKINGLTESDFYMAA
                     KADREFEPFRAAAS"
     gene            complement(4170..5165)
                     /locus_tag="VI359_01900"
     CDS             complement(4170..5165)
                     /locus_tag="VI359_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY6973035.1"
                     /translation="MAEKKPKSVRPVGSRQRRKPAGTSTGLAASELQAAAPPSEVAAL
                     HRAIDDDGGKVLAIYREPYGGRWLVLSALPIELVEPTPYQRNLSDMHVRKLEAVIGKI
                     GRFLDPIIAVHAPTPDHAAKYWTPNGNHRLSAMRTLGAKSIIAIVVPEAAAAYQILAL
                     NTEKAHNLREKSLEVIRMYKELARLDDATEETYALEFEEPAFITLGLCYEERPRFSGG
                     AYHPVLKRVDDFLKKPLHATLDVRRARAKALLALDDQIVIQVEALKAKGLTSPYLKSF
                     VVARVNPIRFRPKDASPLSFDEALDRMMHAAVKFNPDKIKLGDLARSGGAADEAE"
BASE COUNT         1262 a         1613 c         1601 g         1250 t
ORIGIN      
        1 gcgttggatt tcacattgag cacaagaacg tcatcaatct ggttacagac gccgactgtc
       61 gggccgaaca acgcatcgtt gacgtcattc agaaagccgt tccctcccat cgtatccttg
      121 cggaagagcg tgggttgacc cggcaattac cttctccata ccaatgggta attgatccgc
      181 ttgacggtac cacgaatttc gcccacgggt ttccggccta ctgcgtgtcc attggggtcg
      241 aatacgaagg tcgcggtctt atcggcgttg tgttcgaccc gactcgggat gaactctata
      301 cggctcaacg aggcaagggg gcttttctta atgacacgcc tatttcggtc tcatcagtag
      361 atcatctcga tcgggccttg ctcgtcaccg gattcgcgta tgacattcgc gagacaccga
      421 acaataatct ggaccatttc gtccgttttg ccctgaaagc gcagggcctg cgccgcaccg
      481 gcacagcggc tttggatctc tgttacgtcg cgaccggtcg gttcgatgga ttttgggagg
      541 tcgcgttgaa cccgtgggat atggccgccg ggatcgtgat tctcgaagag gcgggcggca
      601 aggtgacgaa tttcaaaggg aaaacgcatt caatttacga gaaagagctg gtagcgagta
      661 acggtcggat ccacgaggcc atgctcaacg tacttcagca gaaccacccg ctcgcgtgac
      721 acatcggaag cccgatcgca cggccccacg acaagtgact tcgtagaaca tccggctggt
      781 ttctgggaaa aactgctgtg aacggatagc cagatggaca ggcaatgagg tatgatcttt
      841 ccgtcagtgt gaagatgaga gaggatgtat ggcgagctcg acgaacccgt caggcaaatc
      901 aaatggccac ccttcccagt cagctgcagc tgaatctact acgtgggatg tggagctcct
      961 gcgggtcctc gtgagtcgaa agggtacacg cgtcgatgca gaatgggcga tccatcctca
     1021 actgaagcag gatctcctgc caaccgaatg gcaagaggtg accgatctca tgacaaaagt
     1081 taccggcatt gtcggaacca ggttttctca agttctgtca caagccgatc ctgaaccacc
     1141 cagtaacgca tagttacata tccgttccct tcccatccca cctcattgtt caactgtcgc
     1201 cagcccggaa aaaggaggcc gatatggaaa cgtattacca tccccacgat ctcggaaaat
     1261 tcgcagacat gggcaaaggc aaccaagctc tgtgggagaa gttcatgagt tattacagct
     1321 cggtctttgc cgaaggcgcc ttgacagaac gagagaaggc gctcatcgcc ttggccgtcg
     1381 cgcacacggt ccaatgcccg tattgcatcg acgcctatac ccaggcctcg ctggagaaag
     1441 gatccaacat cgaagaaatg accgaagcgg tgcatgtggc ctgtgcgatt cgcggcggcg
     1501 cctccttggt ccacggcgtt cagatgcgca acgtcgccga gaaactttct atgtaggaaa
     1561 gcggtcggtc ttgaaaccct cggaaactcg atgccacagg atgctcaaga acgctgtcat
     1621 tctcacccgc ccggcccgag cgactattta cacccgcccg ccctccgagt gcgtggcact
     1681 cgatagcccg ggacgcgctt tgccccaatc aagcccgcag ggagactgga cactgagtcg
     1741 tacggttgtc ggcacgttga agtgggcagg cgaccgagaa cgcagccagc ggcgttttca
     1801 gcatcctgct agaggtacgg gttttcggga atcgcgaggg tgaagtactt gccgcgttct
     1861 tcgtagagga tacgacgggc tgtcagcgac gccaatgcgt ccgccagtcg actcgcggaa
     1921 cacgagcagt caggacgctc tgccagaatc gaatggatct gatcctcact cttcgccgct
     1981 tcgttgcagg cctcaataat gccggcctca ggctggtcaa atcggtgtcg catgggagcc
     2041 tttggattcc gcccatcata gacggtaatg tagcccatcg cctttgagta ataaagaaac
     2101 ggtcggtcgg cggatgcatg cagacgcttc catgccccga cgacatcgac gagttcctga
     2161 tacacgtgtg gatccacagg ccagttatcc aattcgtact cgaaatcata cgcaattttc
     2221 ttcagatcca cctggcgccc atcgtacaca tactcatagg ccaggcccgg tcctgtaatc
     2281 cgcactccat agtcatgtgg tcgcttgtag tagggactga accgttcaag ccagaatttc
     2341 ccggtcgcct cgggcggctg gagatggaat agcgccggga tcaggtcgat ctgtcgtcga
     2401 taatcctcgg tggtttctcc aggaaatccg aggaggatat tccacgatac ctcgacgtgg
     2461 taatagaagc tccacttcag acagatgatg ttctgcatgg gcgtgacgcc cttatccatc
     2521 gcccgaagct ggttcaagct gagactctcc agacccggct gcatgcattt cacccctccg
     2581 gccgccaagg tgcgaatttg gttcttgtga agattgctct tggtctcgat gaacacatca
     2641 agatcacagt gatcggcggc aaacttcccg aagaggttct cgatgtactt catatcgatg
     2701 atgttgtcca cgaggcggaa ccgcgctgtg tcgtaccggc tggacaaata tgccatttcc
     2761 cgcgcgactt gttccgatga cttggcgcgg aacctcatgc tttgcgcatt gagcccgcag
     2821 aacgtgcagt gatgtttctc gccccaccag cagcctcgcg acccttcgta caagagaatg
     2881 cgatcgagcc cttgcgcagc ctctttgcca agttcggcca acaaacgata gtagtcgtcg
     2941 taatcggggg gaccagtctg tgtgaaatcg gaaaagagcg actggttggg ctcatatcga
     3001 atctggccgc tctgccgata agtcactcct ggaggcggaa cgccggtttt tccggaaagc
     3061 acatatgtca ctagcgcggg gaacgtgtct tccccttcgc cgaccaccac atggtcgatc
     3121 caggggaacg cccggaaatg ctccagaccc atttcaccat cgaagttcgc cccaccgaag
     3181 acgatcgtga cgtgcggata caggtccttg atcaacttcg ccattgtcag gcttgccacg
     3241 ttttgatcga acgtcgatgt gaagccgaca agcttatatt gcccccagtc gatcgccgtc
     3301 atcgcccagg tcaagaactg tggagccgta cggccggcca tgtcttcgaa gaacgacatc
     3361 gggtggccac tctcctgagt aatctgctcg aaaaccggct tgaacatccg cgggtattcc
     3421 gcgtgcttcg ggttgtctcg gaagagaaga tgcgagaaca gccattcgcc gaacagggct
     3481 cgtttctcgc agatcaattc atagagcggc actccgatct tgtgagcgaa tcggacattc
     3541 agatgatggc agtcgaccgg aatccctttg gattttagga gtgcagaaag tgtcccgagt
     3601 tgaatcgagg gatacttcga gaagctgaag ggcatgttga caagcgccac ttgcgccttg
     3661 tcgttcatct ggcgcctcgt ccggatatgg ccacgtctgc caacgcgcgg tcagctagca
     3721 gcagctcgaa acggttcgaa ttcacgatca gctttcgccg ccatgtaaaa gtcgctttcg
     3781 gtgagaccgt ttattttgtg ggtccagatt tccaccttgc acttccccca ggcgaggtac
     3841 aaatcggggt ggtgcccttc tgattcagcg atcgccccca ccttgttgac ataggcaaga
     3901 gcctgggcaa aatccttgaa tgtaaagagc cgttcgagat ggcctgcccc gtttagcgac
     3961 cagccccggc ccaattcctt gaggagagcc tggctacgct caggcggtag cgggggcaca
     4021 cccccgcggc acggaacaca tttgttgtcg gcgaggccca tggcaatacc tccagcaaga
     4081 cgatagacag atcgttcgcg tcttcagcga agtattctaa aggggcgccg agaggaaggg
     4141 caaggtggct tatccggttg gtccgagggt cattctgcct cgtccgctgc gccgcccgag
     4201 cgggccagat cgcccagctt gatcttgtcc ggattgaact tgacggcagc atgcatcatg
     4261 cgatcaagcg cctcgtcaaa ggaaagggga gaggcgtctt tcgggcgaaa ccggatcggg
     4321 ttcacgcgcg ccaccacaaa gctcttcaga taggggctgg tcagtccctt tgctttgagg
     4381 gcctcgactt gtatgacaat ttgatcatcg agcgcgagca gagcttttgc tcgcgctcgc
     4441 cgcacatcta atgtcgcgtg aagcggtttc ttcagaaaat catcaactcg cttcaagacc
     4501 ggatgataag ctcccccact gaatcgcggc cgctcctcat agcagagccc cagggtaatg
     4561 aaggctggtt cttcaaattc gagtgcatag gtctcctcgg tggcatcatc cagtcgagcc
     4621 agttccttgt acatgcgaat cacttccagc gacttttctc tcaggttatg ggccttctcc
     4681 gtattaagcg cgagaatctg ataggcagcg gcggcttccg gtaccacgat ggcaatgatg
     4741 ctcttggctc cgagcgttcg catcgcggac agccgatggt ttccgtttgg cgtccaatac
     4801 ttggctgcat ggtcaggcgt gggtgcgtgc accgcgatga tcggatcgag aaatcgcccg
     4861 atcttcccga tcacggcttc cagtttccgc acatgcatgt cagacaggtt gcgctgatag
     4921 ggcgtcggct ccaccagttc gatcggcaag gcgctgagga caagccatcg cccgccgtag
     4981 ggttcacgat agatcgcgag gaccttaccg ccatcgtcat cgatagcccg atggagggct
     5041 gcaacctcgc ttggtggtgc cgcagcttga agctcgctcg cagctaatcc cgtggacgta
     5101 ccggccggct ttcgccgctg gcgtgaaccg accgggcgaa cagacttggg cttcttctca
     5161 gccatgatga cgtcataaat ggtagggaag tatcggcaaa aaggaaaggc ctgctagacg
     5221 aattcctgtc gaaacaggct cgtcaaaatg cattattctc acccgcccac cccagcgtgc
     5281 caagacacgc ttgtctccgg caaggcggca gcgaatgaca ttacaaggcg tggtttcccg
     5341 tgcaggtaaa cacctatgct gattcgtagg atcgtcaaaa cggcttttaa cgaggccgca
     5401 gcaaacgaaa gggcgaaggc gtacttagtg ctgtacgtcg agcccttgag tgcagcgaga
     5461 acgaagttaa aaggtgtttt cacgatccta gatacagcac gggcgaccct gcacgatcgg
     5521 tgggaggtca cccgtgattg gcgcttagga cgccggaact cttgttcgat tgtttcggcc
     5581 cagtgagacc cacgcgaacg tcattggtgc ttaggcttct ggtcggggga acgctcagtt
     5641 cccaggcgct tgtgttcctc aacgccagcg acggattctc acatcagatt gaggctgaat
     5701 tccagttggg ctctggtgcc aagagg
//
LOCUS       DATDNW010000072         4346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1031701, whole genome shotgun sequence.
ACCESSION   DATDNW010000072 DATDNW010000000
VERSION     DATDNW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031701"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="bioA"
                     /locus_tag="VI359_01905"
     CDS             <1..1047
                     /gene="bioA"
                     /locus_tag="VI359_01905"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY6973036.1"
                     /translation="SRVFYSDDGSTAVEVALKMAVQYWQQRNPAAGPKNTFLHLKLAY
                     HGDTIGAVSVGNIEMFHSRFKPLLFPTLSADPPYCYRCPLRLTYPSCQIACLEPIEQI
                     LRARHRELAGFIIEPLVQAAAGMITSPPGYLKRIRELCTKYGVLLIADEVATGFGRTG
                     RMFACQHEDVTPDLMAISKGLTGGYMPLAATLTTEEIYDVFLGKYEDFKTFFHGHSYT
                     GNPLGCAVALANLTVFRKDRTLTKLRPKITELKRLIRSLANLPHVGEIRQQGFMVGIE
                     LVKDRATKEVYPLETRIGHRVTDEARRRGLLIRPLGNVIVLIPPLSTSIRELRQMMGT
                     MAASIRAATNPTAD"
     gene            complement(1095..1895)
                     /locus_tag="VI359_01910"
     CDS             complement(1095..1895)
                     /locus_tag="VI359_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY6973037.1"
                     /translation="MPHPLGLITLLTDFGDRDYFVGSMKGVILTINPQTTIVDLSHHI
                     VPHAVEEAAHILKCCYACFPEGTVHVVVVDPGVGSLRRPLLVKTNRYFFLAPDNGILS
                     YILDEENQIEAREITNRQFRLESPGTTFDGRDLFAPAAAWLTKSQPVSAFGPVVHDVQ
                     TFPISKPRWESGMLVGEVVYIDRFGNLISNITAAKLKELEAVTKRPNPLIRMAGHLIR
                     GLVGSYSEGSPEEPCALINSNGYVEVFLKQADVAQLLKIRRGEQLTIS"
     gene            complement(1897..2373)
                     /gene="greA"
                     /locus_tag="VI359_01915"
     CDS             complement(1897..2373)
                     /gene="greA"
                     /locus_tag="VI359_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY6973038.1"
                     /translation="MPTPITKKGYEALKAELDRLRKVERPRVIEAIAEARSHGDLSEN
                     AEYDAAKERQGFIESRIAEIESKLADARVIETAGRTSDTVVFGATVLVVEQESQSKKQ
                     YTLVGQDEADMKFNKISVQSPVGRALIGKRVGDFVEVKTPVKLVEYEVVEIRFEEL"
     gene            complement(2389..>4346)
                     /gene="carB"
                     /locus_tag="VI359_01920"
     CDS             complement(2389..>4346)
                     /gene="carB"
                     /locus_tag="VI359_01920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY6973039.1"
                     /translation="LRTPTPERLWHVADGMRLGLSNEELFALTKIDPWFLEQVRELLQ
                     FEERLVGQAGTSSAGVDDALLWEAKELGFSDDRIAQLIGVTATSVSKQRLQALQSRRV
                     TYKRVDTCAAEFEAYTPYLYSTYGHECEARPTDRKKVVILGGGPNRIGQGIEFDYCCV
                     HAAMALRDEGIETIMVNCNPETVSTDYDTSDRLYFEPLTEEDVLNIVQCEQPLGVVLQ
                     FGGQTPLKLALPLAKAGVRILGTSPDAIDRAEDRERFRDLLNKLGLRQAASGMARSVE
                     EAVQVASRISYPVMVRPSYVLGGRSMQIVYDEAGLLAYMGSAVKASPKHPVLIDKYLS
                     DAIEVDADAISDGKHVVVAGIMEHIEEAGVHSGDSACSLPPYSLDQKIVEEIRRQMTA
                     LALELGVVGLMNAQFAVKDNTVYVLEVNPRGSRTVPFVSKAIGVPLAKMAMKTMVGKS
                     LRELGFTEAHTPTHLSVKEAVFPFTKFSGVDVLLGPEMKSTGEVMGMDADFGWAFAKS
                     QAGAGATLPAGGTAFISVKDSDRPVALEVARQLTGLGFKIESTKGTAAYFREKGLDVE
                     TVNKVREGRPHIVDHIKNGQITLVVNTVRTASSHQDSLSIRREALIKGLAYFTTMRGA
                     RAAVMGIEAMLKKELTIRSLQEYHRLS"
BASE COUNT          993 a         1293 c         1077 g          983 t
ORIGIN      
        1 tctcgcgtct tctactctga tgatggatcg accgcagtcg aagttgcgct caaaatggca
       61 gtccaatatt ggcagcagcg gaatccggcc gcagggccca agaatacctt ccttcacttg
      121 aagctcgcct accacggcga cacgatcgga gcggtcagcg tcgggaatat cgagatgttc
      181 cattctcgat ttaaaccgtt actattcccg acactcagcg ccgatccgcc ctattgttat
      241 cgctgcccgc tgaggctgac atacccctcc tgccagatag cctgcctgga accaatcgag
      301 cagatactca gggctcgaca tcgggagttg gccggattca tcatcgaacc cctggttcag
      361 gctgcggctg gtatgatcac gtcaccacca ggctatctca aacgaatccg cgagctttgc
      421 acgaaatacg gcgtgctctt gatagccgat gaagtagcga caggattcgg ccgaactgga
      481 agaatgttcg cctgccagca tgaagatgtc accccagatt tgatggccat cagcaaaggc
      541 cttaccggcg gctatatgcc attggccgcc acgctgacta ccgaagaaat ctacgacgtg
      601 tttcttggaa aatacgaaga ctttaaaacc tttttccacg gccacagtta caccggcaac
      661 cccctcggtt gtgctgtcgc cctggcgaat ctaaccgttt tccgaaaaga caggacgctc
      721 acgaaacttc ggccgaaaat taccgaactg aaacggttga tacgctccct tgcgaatctg
      781 cctcacgtcg gcgagattcg gcaacaagga ttcatggtag ggattgagct ggtgaaagac
      841 cgcgccacga aggaagtcta tccgctcgaa accagaattg gccaccgcgt gacagatgaa
      901 gcccgcaggc gggggcttct aatcagaccg cttgggaacg tgattgtgtt gataccaccg
      961 ctcagtacct caattcgcga actgcgtcag atgatgggaa caatggctgc atcaatccgt
     1021 gccgctacca atccaacagc tgattgacag cgcacgatac accggaatca acggagcgac
     1081 gatctgatgc ctcattagga aatcgtaagc tgttcgcctc gcctaatttt caacagctga
     1141 gcgacgtctg cctgcttgag aaacacttct acgtacccgt tgctattgat caaggcgcac
     1201 ggttcttctg gactcccctc gctgtagctg ccgacaagtc cccggatcag atgaccggcc
     1261 atgcggatca gcgggttcgg ccgtttcgtg actgcttcaa gttctttcag cttggccgca
     1321 gtgatgttgg aaattagatt gccgaaccgg tctatataga cgacttctcc caccagcatt
     1381 cccgattccc aacgcggttt gctgatcgga aacgtctgaa catcatggac caccggacca
     1441 aacgcagaga ccggctgact tttggtgagc caggcggcag caggagcaaa aagatctctg
     1501 ccgtcgaagg tcgtccctgg tgattctagc cggaattgtc tgttggtaat ttctcgagct
     1561 tcaatctggt tttcttcgtc gagaatataa ctcaagatgc cattgtccgg cgccaagaaa
     1621 aagtatcgat tggttttaac caaaagggga cggcggagac ttcccactcc tgggtccact
     1681 acaacaacat gaacagtgcc ctcaggaaaa cacgcataac agcatttcaa gatgtgggca
     1741 gcttcttcga cggcatgcgg cacgatatga tgggaaagat cgacgatcgt cgtctgcgga
     1801 ttgatggtga ggatgacccc cttcatgctc cccacgaaat agtcccgatc gccgaaatcg
     1861 gtcaacagcg tgatgagccc gaggggatgg ggcacgctac aactcctcga accgaatctc
     1921 caccacttcg tattcgacca acttcacagg cgtcttgacc tcgacaaaat cacccactcg
     1981 cttgccaatg agggcacgcc caacaggcga ctgcacggag attttgttga atttcatgtc
     2041 cgcctcatcc tggcccacaa gagtatattg tttcttggac tgagactcct gttcgaccac
     2101 cagaacggtc gcgccaaaga ctactgtgtc cgacgtgcgc ccggcggttt caatcacgcg
     2161 ggcgtcggcc agcttcgatt cgatctcggc gattcgcgac tcgatgaatc cttgtcgctc
     2221 tttcgccgca tcatactcgg cattttcgct caaatcaccg tgggaacgcg cttccgcaat
     2281 ggcctcaatg acccgaggcc gctccacttt tcggagtcgg tccagctcgg ctttcaacgc
     2341 ctcatatcct tttttggtaa tcggtgttgg catgataacg gaattccttt atgacaggcg
     2401 gtgatattcc tgcaacgacc ggattgtcaa ctctttcttg agcatagcct cgatacccat
     2461 caccgcggct cgggccccgc gcatggtcgt gaaatacgct agacctttta tgagagcctc
     2521 gcgccgaatg gagagcgagt cctggtgaga ggatgcagtc ctgactgtgt tgacaactaa
     2581 cgtgatctgc ccattcttaa tatggtcgac gatatgcggg cgcccctctc ttactttatt
     2641 gaccgtttcc acatcgagtc ctttttcacg gaaataggcg gctgtaccct tcgtgctctc
     2701 aattttgaat cccaatccag tcagctgtct ggcaacttct agcgccactg ggcgatccga
     2761 atcttttacg ctgataaaag cggtccctcc cgcggggagc gtggccccag ccccggcttg
     2821 agacttggca aacgcccatc cgaaatctgc gtccattccc atgacttcgc cggtggattt
     2881 catctccggc cctaggagta catcgactcc ggagaacttc gtgaatggaa agacagcctc
     2941 tttcacagac aaatgggttg gtgtatgcgc ctcggtaaat cccaattctc gtaaactttt
     3001 tcccaccatc gttttcatcg ccatcttggc aagcggcacc ccgattgctt tactcacaaa
     3061 cggcacggtt cgagaaccgc gcggattcac ttcgagcaca tacaccgtat tatccttcac
     3121 ggcaaactga gcgttcatga gtcccacgac gccaagttcc agggccagcg cggtcatctg
     3181 acgacggatc tcctcgacga tcttctggtc gagcgagtaa ggaggcagcg agcaggcaga
     3241 gtcacccgag tgcacgccgg cctcttcgat atgctccatg atgccggcga ccacaacgtg
     3301 tttcccgtca gaaattgcgt cggcatcgac ctcaatcgca tccgataagt acttatcaat
     3361 gaggacggga tgcttcggcg acgccttgac tgcagacccc atgtaggcaa gtaagccggc
     3421 ctcgtcatag acaatctgca tagaccgccc gccaagaaca tacgacggcc gtaccataac
     3481 gggataggag atacgcgacg ccacctgcac ggcttcctca accgatcggg ccatcccgct
     3541 cgcggcttgc cgaagtccca gcttgtttaa gagatcacgg aaccgttcac gatcttccgc
     3601 cctatcaatt gcatccgggc tggttccgag aatcctcacc cccgccttgg ccagcggtag
     3661 cgctagcttg agtggtgttt gcccaccaaa ctgtaagacc acacccagcg gctgctcgca
     3721 ctgaacaatg ttcaaaacgt cttcctctgt cagtggctcg aaatagagtc gatccgaggt
     3781 atcgtaatcc gtgctgaccg tttccggatt acaattaacc atgatcgttt cgatcccctc
     3841 gtctcgcaac gccatcgccg catgtacgca gcaatagtcg aattcaatcc cctgcccgat
     3901 tcggtttggc ccgccgccaa gaatcaccac ctttttccgg tccgtcggcc gcgcttcgca
     3961 ctcgtgtcca taggtcgagt agagatatgg ggtataggcc tcaaattctg cggcacaagt
     4021 atccacccgc ttatacgtca cccttctgga ttgcaacgcc tggaggcgct gcttcgagac
     4081 agaggtggcc gttacaccga tcaattgtgc aatccgatca tccgaaaagc ccagttcttt
     4141 ggcttcccac aacaatgcgt catcgacgcc agccgaactg gttccggcct gaccgactaa
     4201 tcgttcctcg aattggagca gttcccggac ctgttccaaa aaccagggat cgatcttggt
     4261 caaggcaaac aactcttcat tggagagacc gaggcgcatt ccatcggcca catgccacag
     4321 ccgttcggga gtcggcgtcc gcaaca
//
LOCUS       DATDNW010000073         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_147155, whole genome shotgun sequence.
ACCESSION   DATDNW010000073 DATDNW010000000
VERSION     DATDNW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147155"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2753)
                     /locus_tag="VI359_01925"
     CDS             complement(<1..>2753)
                     /locus_tag="VI359_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973040.1"
                     /translation="RESTVRLFADITAKGLYDTRRRFLGTIGVIRDLSAQKQHENTLQ
                     QLQQHVRHSDKIQTLVQRITEFSRNLQSPLTAVLDDSRHLLEALRGLRLEERIDSLTG
                     HATAAVALGQQVAETLRESAILAGTGTVNDLLNEVLETMRIAGVDTDAIVTQLSGQLP
                     PYENDRDKTRTLLRQLIRYAQAYLRTVGRASGLIVMTHGVGVPSRIPETPALFPLSPP
                     TEVEVEIRESDRGHPPDVPPGQTEAVDLLTAYQLVRDLSGTLDFTAPVHGPLRIVVRL
                     PVSPRVIQETPPSKPTTPIPLPPAAAPITTPSVPTIEPPAVQPVVLERRQSTRISTVL
                     PAHVTLGSTTWVGTITNLSTGGACLILPPDMPSLDIQAAMVALKTEVSILELGGTAYW
                     RTAATVPLEHHAPSHHLVLVFSAPQPQEAAVLASLVEAARERSLAFTLEAQLSVAPQS
                     QPLETTAAPTVGVETDQREAVRLRFTLPARLDVTDQFGSTRRLSALVIDMSRTGACLE
                     IHPPPGPLSGSVTLHFTDAHISTSPAADGPTAPDAVLPARIIWMTGISPAQRTISASM
                     FESALRLGLCFHALTPYAEREINRVLSRRLTSDEGTGDTLLRRTPVISIPRECRNQRG
                     QTIAITDDHLRHPLTSNLPVVIIAPGYGQTAADYAAFGHFLAHHRLRVLRYDHTNHLG
                     WSEGELQHTSLRSMQADLIKIIEFVQHTWAGAPIAVVASDMAARAALKVAGQSPSIAL
                     FVLVNMAVDLQLTLQTVHTHDLVTDYRFGLRRGITNLLSLNINLDHFVGDAIAGNFTD
                     LASTLEDLRLTRVPLAVVTAPVSSLSPLPPSDLPHTFITALGTRNRVISVPAALTEPH
                     MTFGSPSVVAFQQILGQLTSAMTLPENPVELQDQAQRSLNRQRRIEMERTRLCHGVSG
                     ATR"
BASE COUNT          602 a          660 c          910 g          581 t
ORIGIN      
        1 ctcgagtcgc gcctgataca ccatggcaca ggcgtgttcg ttccatctcg atccggcgtt
       61 gacggttgag cgaccgctga gcctgatctt gaagctcgac cgggttttca ggaagtgtca
      121 tggcagaggt gagttggccc aggatctgct gaaatgccac gacagatgga gatccgaacg
      181 tcatgtgcgg ttccgtcagc gccgcgggta cgctgattac gcggtttcgt gttccaagcg
      241 cggtgatgaa ggtatgtgga aggtcggatg gtgggagggg actcaaagag ctgacgggtg
      301 ccgtgactac cgcaagggga actcgagtca gtcgaagatc ttcaagggtt gaagcaaggt
      361 ctgtgaagtt gccggcgatc gcgtcaccga caaaatgatc gaggttgatg ttcaggctca
      421 acagattcgt gatgccacga cggagtccaa aacggtaatc agtcacgaga tcatgcgtgt
      481 gaacggtctg gagtgtcagc tgaaggtcga cagccatgtt aactaacaca aagagggcga
      541 tggaaggcga ctgtcccgcg actttgagcg cagcccgggc tgccatgtcg ctggcgacaa
      601 cggcaatagg cgcaccggcc cacgtgtgct ggacgaactc gatgatcttg atgagatctg
      661 cttgcatgct tcggagcgac gtgtgctgta gttcgccctc gctccaaccg agatgattgg
      721 tatggtcgta tctcaggaca cggaggcgat gatgggcgag aaagtgtcca aaggctgcgt
      781 agtctgcagc ggtttggcca tagcctggtg cgatgatgac tacagggagg tttgacgtga
      841 ggggatggcg gagatgatca tcggtgatcg caatagtttg cccgcgctgg ttccggcatt
      901 ctctgggaat actgatgacc ggtgttcggc ggaggagggt atcccctgtt ccttcgtcgg
      961 acgtcaaccg ccgggacagt acacgattga tttcccgttc agcataggga gtaagagcat
     1021 gaaagcagag gccaagtcga agggccgatt cgaacattga ggcagagatg gttcgctgcg
     1081 caggtgatat tccggtcatc caaatgatcc gagctggaag caccgcatcc ggcgcagttg
     1141 gcccatcggc ggctgggctg gtggaaatgt gagcgtccgt aaaatgcaat gtgacagaac
     1201 cagatagcgg accgggcgga gggtggattt cgagacaagc gccggtccga ctcatgtcga
     1261 tcacaagagc agagagtcgt ctggtggatc caaattgatc ggtcacatcg agacgtgctg
     1321 ggagagtgaa tctcagacgg acggcctccc gttgatcggt ctcgactcca acagttggtg
     1381 ccgcggtggt ctcaagcggt tgcgactggg gggcgacgga aagctgagct tctagtgtga
     1441 atgcgagtga acgttcgcgt gcggcctcga ccagagaagc gaggacagcc gcttcttgcg
     1501 gctgaggagc agaaaatacc agaacgagat ggtgactggg tgcatggtgc tcaagtggaa
     1561 cggttgcagc agttcgccag tatgcggttc cgccgagttc aagaatgctg acctcagttt
     1621 tcaaggcaac catggcagcc tgtatgtcca aagacggcat atctggtggc aggatgagac
     1681 atgccccgcc cgtgctcagg ttcgtgatag taccaaccca tgtggtcgaa ccgagcgtca
     1741 catgagcggg aagaaccgtc gaaatgcgtg tactctgtcg gcgttccagc accacgggct
     1801 gaaccgcagg aggctctatc gttggaacgc tcggggtggt gatcggagca gcagcgggtg
     1861 gtagtggtat tggagttgtt ggtttggatg gcggcgtttc ctgtatgacc cgaggagaaa
     1921 caggaagacg aacgacaata cgaaggggac cgtgaaccgg tgcagtgaaa tccagcgtcc
     1981 cgctcagatc tcggacaagc tgataggcgg tgagaaggtc gacagcctcc gtttgcccag
     2041 gaggaacgtc aggcggatgc ccacgatcag actcacggat ttctacttcg acctcggttg
     2101 gtggtgagag ggggaacagt gccggagttt cgggtatccg cgatggaacg ccgacaccat
     2161 gtgtcatgac tatcaatccg gaagcccgac cgaccgtgcg taagtaggcc tgggcatatc
     2221 gaatcagctg gcgaaggaga gtgcgagttt tgtcacggtc gttctcgtag ggcgggagtt
     2281 gtccagacaa ttgagtgaca atggcgtccg tgtcgactcc cgcaatgcgc atcgtctcga
     2341 gaacttcgtt cagaaggtcg ttcacggtac ctgtgccggc gaggattgcc gactcgcgta
     2401 gggtctcagc gacttgttgg ccgagtgcca ccgcagctgt tgcatggccg gtcagggagt
     2461 caatacgctc ctcaagccgc aaaccacgga gcgcctcaag gagatgccga gaatcatcga
     2521 ggacggcagt aagcggtgat tggagattgc gcgagaattc tgttatgcgc tgaacgagtg
     2581 tttgtatttt gtctgagtgc cgtacatgtt gttgcaattg ttgtagtgtg ttctcgtgtt
     2641 gcttctgcgc ggagagatcg cgaatcaccc cgatcgtccc cagaaaccgg cggcgagtgt
     2701 catagagccc tttggccgtg atgtctgcga ataagcgaac ggtcgattct ctt
//
LOCUS       DATDNW010000074         2347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_827785, whole genome shotgun sequence.
ACCESSION   DATDNW010000074 DATDNW010000000
VERSION     DATDNW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827785"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VI359_01930"
     CDS             complement(<1..561)
                     /locus_tag="VI359_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY6973041.1"
                     /translation="MSLPTLHKQYRLIAILLCFFFSWNWLSASAQMGKPEVLYYKSWA
                     VVIGIENYLVAPKLSGAIEEGKTIAQTFRQLGFEEVVELYDKDASLRRLQQTLTDFLP
                     RKVGRQDRLIVYFVGHTGITQDPAGKDLGYLVPWDAQLGTVSKSITFEQLKEFTRRTA
                     SKHTLLIFDAAIRGWEETAAQQLSLEG"
     gene            812..>2347
                     /gene="acs"
                     /locus_tag="VI359_01935"
     CDS             812..>2347
                     /gene="acs"
                     /locus_tag="VI359_01935"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY6973042.1"
                     /translation="MADNIDTLLKESRIYQPTPATRAAAYIQDYETAYAKSIADPEQF
                     WAEAAKQLEWFRPWSKVLDWNYPWAKWFVGATCNISYNCLDRHLKAGRKNKVAVIWVG
                     ENDQERVFTYGELSRQVNRCANALKALGLQKGDRVTIYLPKIPEQIVAMLACARIGVI
                     HSVVYSGFSAPALATRIQDAEARVVITADVGYDRGKVIQLKPVVDEAIAHCKSVERVV
                     VVRRQKPEISLSASKEIDWHDWLRPEKALCPAEQLDSEDPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYLTTKYVFDLKDDDVYFCVADPGWVTGHSYIVYGPLLNGATILTAEGKP
                     DYPNPGRWWDLIERYGVSIFYTTPTAIRLLMRYGEDWPKKFDLSTLRILGSVGEPINP
                     EAWEWFHRVTGDDKPIMDTWWQTETGSILVTPLPTVPLKPGSATRPFLGIEADVVDRE
                     GKSLPVNAGGFAVIKKPWPSMMRTIYKDPERYKTYWNTIPNCYTAGDVCHKDADGYMW
                     FMGR"
BASE COUNT          550 a          688 c          611 g          498 t
ORIGIN      
        1 tccttccagc gacaattgtt gagcggcggt ttcttcccat cctcggatcg ctgcatcgaa
       61 gatcagcagc gtatgtttcg aggccgtccg cctggtgaac tccttgagct gttcaaacgt
      121 gatcgacttt gagacagttc caagctgagc atcccatggc accaaatatc caagatcctt
      181 cccagccgga tcctgcgtga tgccggtatg cccgacaaaa tacacgatga gtcgatcctg
      241 cctcccgact tttctcggca agaaatccgt gagggtttgt tgcagacgac ggagactcgc
      301 atccttgtca tagagttcga cgacttcttc aaatccaagc tgtcgaaatg tttgggcgat
      361 ggttttgcct tcctcgatcg cgccagaaag tttcggcgca accaggtagt tctcaatacc
      421 gatgaccacc gcccaggact tataataaag gacctccggc tttcccattt gtgcagaggc
      481 agaaagccaa ttccagctga agaagaaaca caagagaatg gcgataagcc ggtattgctt
      541 atggagtgtt ggcaaggaca tcgaggaact gtataggtga acagccgtac aaaatcagcc
      601 tactctggcc ccacaatcac tgtcaaggaa acggacctgc ttgttgcccc ccaaatccac
      661 gaatctcaag gtgtttgtcg aagccattgc actggccccg taccaaggta cagttgcagt
      721 tccgcatgat ctttccgcat aatgccgatc gggcttttct ccgcaagcct gttcgagcct
      781 ttacacctgc ccttcgaagg agcacccgtc tatggccgac aacatcgaca ccctcttgaa
      841 agaaagccgc atctatcagc cgactcctgc caccagagcg gccgcttaca ttcaggatta
      901 cgagacggcc tatgccaaat cgatcgccga tcctgaacaa ttctgggcag aggcggcgaa
      961 gcagctcgag tggtttcgtc cctggtcgaa ggtgctcgac tggaattatc cctgggccaa
     1021 atggtttgtc ggcgcgacct gtaacatctc ctataactgc ctcgatcgac atctcaaagc
     1081 cggtcgcaag aacaaggtgg ccgtcatttg ggtgggagaa aacgatcagg agcgggtctt
     1141 cacctatggc gaactgtcac gtcaggtgaa tcgctgtgcg aacgctctca aagcgttagg
     1201 actccaaaaa ggcgaccgcg tcaccatcta cctcccaaaa attcctgagc agatcgtcgc
     1261 catgctcgcc tgcgcccgca tcggggtcat ccatagcgtg gtctactccg gattcagcgc
     1321 gccggcctta gccacgcgaa ttcaggatgc tgaggcccgc gtcgtgatca cggccgacgt
     1381 tggatacgac cgcggcaaag tcattcagct gaagcccgtc gtcgacgaag ccatcgccca
     1441 ttgcaaatcc gttgaacgcg tcgtagttgt acgccggcag aaacctgaaa tctccctatc
     1501 cgcctcgaag gaaatcgact ggcacgactg gttgagaccg gagaaggccc tgtgtcccgc
     1561 ggagcaactg gactccgaag atccgcttta tatcctttat acgtccggca caaccggcaa
     1621 acccaagggc gtcgtgcatg tccatggcgg atacatggtc ggcacctacc tcaccacgaa
     1681 atatgtcttc gacctcaaag acgacgacgt gtacttctgt gtcgccgatc ccggttgggt
     1741 cacgggccac agctatatcg tctacggccc tctgctgaat ggcgcgacga tcctgaccgc
     1801 tgagggaaaa ccggactatc cgaatccggg ccggtggtgg gacctcatcg agcgttacgg
     1861 cgtctcgatc ttctacacca cccctacggc gattcgcctg ctcatgagat acggggaaga
     1921 ttggccgaag aaattcgatc tctccacact ccgcatcttg ggtagtgtgg gtgaaccgat
     1981 caatccagaa gcctgggaat ggttccatcg cgtcaccgga gatgacaaac cgatcatgga
     2041 cacctggtgg cagacggaaa caggatccat cctcgtcact cccttgccaa ccgtgccctt
     2101 gaaaccaggc tccgcaacca gaccgttcct cggcatcgag gccgacgtgg tggatcgcga
     2161 agggaagagt ctgcctgtca atgccggtgg gttcgccgtc atcaagaaac cctggccctc
     2221 aatgatgcgg acaatttata aagacccgga gcgctataag acctattgga atacaattcc
     2281 gaactgctat acagctggcg acgtgtgcca caaggacgcg gacggctata tgtggttcat
     2341 gggccgc
//
LOCUS       DATDNW010000075         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_487367, whole genome shotgun sequence.
ACCESSION   DATDNW010000075 DATDNW010000000
VERSION     DATDNW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487367"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1795
                     /locus_tag="VI359_01940"
     CDS             206..1795
                     /locus_tag="VI359_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973043.1"
                     /translation="MNRSPRGWFRHLSIGQKFLVSFGLILILLVLSLTALLFYLTRIN
                     SYVDRHKRITVPAIVTAANMQRDAYDLKLALHLHDGRGSPDSVKDTMTQLDRHSTAIK
                     TALALYNGTHAARTHPVLYGMLTEHHRLDLAEQEDRALADIRAALDELSINGRKFLES
                     PTRAQRALDQIDQIVTRMITGLNQLVDAHTQIDIEMKHEGDRLLDRARLIALSLALIL
                     ALVIAATYVLTTRHITDPLMTLAATADRVAHQDLSATFEPWPSRDEVGTLATSLMSML
                     ASLRERGTALMRKTKELEAFTYSIAHDLKGPLREIEGFSSLLEKHFSESGDPQVKHHI
                     DIIRKCSLRLTHMIDALLKYSRLEQQDLPRSRFNLLEMINQLVRERQTQAVGISPTIT
                     VDLPFSHLYGESVSIRQVLTNLLDNAIKFSRHSATPEIRISGQQTTTGRVISIGDKGI
                     GFDPAQADKIFGLFERLHPVNEYEGTGVGLAIVKMVMDKHGGRVWAESTPGQGSTFYL
                     AFPEDQQAVNRQVSTSETSPG"
     gene            1828..3171
                     /locus_tag="VI359_01945"
     CDS             1828..3171
                     /locus_tag="VI359_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973044.1"
                     /translation="MRALIIDDEEYVRLVLEQALREEGCEVTTAKNGQAGIDALQTAP
                     FDCVITDLRMPGLDGRAVLNWVKEHQPSIDVLMLTGHGDIKDAVDAIKHGAWDFLVKD
                     TPFDATVVKAALTKLKTVRALRRENLAARHGGFTRDIIAEGSSLAWRKLKVQIAQVAQ
                     ANAPVLIQGETGSGKEVVARLVHDLSRRAHGPFIAINCGAVSRELLESELFGHEKGSF
                     TGATAAKLGLIAATEGGTLFLDEIGEMPGPMQVSLLRFLDRGEYRPVGSTRTLHADVR
                     IVGATNQDLQELILQGRFRDDLLYRINTITLHVPPLRERKEDLPGLVDHILQTLRVPG
                     ATKRTLSPEAIQQLSSYSWPGNIRELRNVIERIVLMSTGTTPVSREEIAQVLPRRQDS
                     RQAGDVTQLSLEEVERLHIMHVLDASGGNKTQAAKTLVIDYKTLLSKLKKYGLGT"
     gene            3326..>3674
                     /locus_tag="VI359_01950"
     CDS             3326..>3674
                     /locus_tag="VI359_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973045.1"
                     /translation="MSQPPSQARRLIDLRKHPRLYTPSGALFSFKRLVVPVQFEGNAE
                     GEGTLIDLSLGGCRLLSDTPLEIGELYNLILQVSKESSPVIIEAAAVRWTQANTYGLK
                     FLSVQSVDESHLRD"
BASE COUNT          845 a         1088 c          980 g          761 t
ORIGIN      
        1 aactgtggtg gggatcgagc cggccatcca attcagattt accgactccc tcgtcggcgc
       61 cgttggagtg ttatttaccg ttgcgggaca gaatgccgcc gacgcgattt atcccaactt
      121 ctcgctccag tggtattgga ataagaccgg caaagccgtg atgagatagc tggcgggact
      181 cctttcgcta tgatacggta gcgccatgaa ccgctctccc aggggctggt ttcgtcatct
      241 ctccatcggc caaaagtttc tcgtctcatt cgggctgatt ctcattttac tcgtactgag
      301 tttgacggcg cttttgtttt atctcacccg catcaacagc tatgtcgatc gacacaagcg
      361 tattacggtt ccggccattg tcactgcggc gaacatgcag cgcgacgcct acgacttgaa
      421 actggcgctg cacctccatg acgggcgcgg ctcgcctgat tcagtcaagg acaccatgac
      481 ccagctcgac cgccatagca cggcgatcaa gaccgccctc gctctctaca acggcacgca
      541 tgctgcacgc acccatccag tcttgtacgg gatgttgacc gagcaccatc ggctcgatct
      601 ggcggagcag gaggatcggg ctctagcgga tatccgtgcc gcattggatg agttgagtat
      661 aaatgggagg aagtttttgg agtcccctac ccgtgctcaa cgggcgctag accagatcga
      721 tcagatcgtg acgcgcatga ttaccggtct caaccaactc gtggatgccc atacgcagat
      781 cgacatcgaa atgaagcacg agggcgatcg cctgctcgat cgtgcccgtc tgatcgcgtt
      841 gtccctcgcc ctcatcttag ccctggtgat cgccgccacc tatgtcctga ccaccagaca
      901 tatcaccgat ccgctcatga cattggccgc gactgcggac cgcgtcgctc atcaggattt
      961 gtcggccacg ttcgaaccct ggcccagccg cgatgaggtc ggcacgctcg ctacctcgct
     1021 tatgtcgatg ctcgcgagcc tgcgcgagcg cggcaccgca ctgatgcgca agaccaaaga
     1081 attggaggcc ttcacctatt ctatcgcgca cgatttgaaa ggcccgctac gggagattga
     1141 aggcttttcc tcactgctgg aaaagcattt ttccgagtcc ggtgaccccc aagtcaaaca
     1201 tcacatcgac atcatcagaa aatgctcact ccgccttacg catatgatcg atgctctgct
     1261 caagtattcc cgattggagc agcaagatct gcctcgaagt cgattcaacc tgttggagat
     1321 gatcaaccaa ctcgtgcgcg aacgacagac acaggcagtc ggcatatccc caacgattac
     1381 agttgacctg ccattcagcc atctttatgg cgagtcggtc agcatccgcc aagtgctcac
     1441 taatttgctc gacaatgcga tcaagttctc ccgccactct gctactccgg aaatccgcat
     1501 cagcgggcag cagacgacaa cagggcgggt gatctcgatt ggcgacaagg gcatcggctt
     1561 cgaccctgct caggctgaca agatcttcgg tctgttcgag cgtctccatc ctgtcaacga
     1621 gtacgaaggt accggagtcg gactggccat tgtgaaaatg gtaatggaca aacacggtgg
     1681 ccgcgtgtgg gcagagtcca caccaggcca gggaagtacg ttttatttgg cattcccgga
     1741 agaccaacag gctgtcaatc gtcaggtctc aacctcggag acgagtccag gctgaatgcc
     1801 gagggctgaa tgctgaaagc ataaaacatg cgtgcgctga ttattgacga cgaagaatat
     1861 gtccgactgg tcttggagca ggctctccgc gaagaaggct gcgaggtgac gacggcaaaa
     1921 aacggacaag cgggtatcga tgcacttcaa actgcaccgt ttgactgcgt gatcaccgat
     1981 ctccgtatgc caggccttga cgggcgcgcg gtgctgaact gggtgaagga acaccagccc
     2041 tctatcgatg tgttgatgct gaccggtcat ggcgacatca aagacgcggt tgatgccatc
     2101 aagcacggcg cgtgggactt tctcgtcaaa gacaccccgt tcgatgccac cgtcgtgaag
     2161 gcagccttga ccaagctcaa gaccgttcgc gcgctcagac gggaaaatct cgccgcgcgg
     2221 catggaggat tcacgcgcga catcatcgct gaaggatcga gtctggcctg gcggaaattg
     2281 aaagtccaga tcgcacaagt cgcgcaggct aacgcacccg tcctcattca gggagaaacc
     2341 ggctcgggca aagaagtcgt tgccaggctg gtgcatgacc taagccggcg agcccacgga
     2401 ccattcatcg ccatcaactg tggtgcggtg agccgagagc tcttagagag cgaactcttt
     2461 ggccatgaaa aaggatcgtt cactggcgcg acggcggcca aactcggtct cattgccgcg
     2521 acagaaggcg gcaccttgtt cctcgacgag atcggagaaa tgccgggtcc gatgcaagtg
     2581 agcctgctgc ggtttctcga tcgcggcgaa tatcgtcccg tcggcagcac acgcacactt
     2641 catgctgatg tgcgcatcgt cggcgcgaca aaccaggacc tccaagaact gattttgcaa
     2701 ggccgcttcc gtgacgatct cctctatcgt attaatacca tcactctgca tgtacctccg
     2761 ctccgtgaga gaaaagagga tcttcccggg ctcgtcgacc acattctcca gactctccgc
     2821 gtcccagggg ccacaaaacg aaccctgtct ccggaggcca tacagcaact ctcatcctat
     2881 agctggcccg gcaatatacg cgaactgcgc aatgtgatcg agcggatcgt gttgatgagt
     2941 accggaacca cgccggtcag tcgtgaggaa atcgcccagg tattgccacg gcgccaagac
     3001 tcacgacagg cgggagatgt gacgcaattg tcattggagg aggtcgagcg ccttcatatc
     3061 atgcacgtcc tcgacgcgag cgggggcaac aaaacccagg ccgccaagac actggtgatt
     3121 gattacaaaa ctctgctcag caaactcaag aaatacggtc tcggcaccta acctcaatca
     3181 gcgccacagg cctcgatctg gcggaactaa ttcccgccgg agtacatttc gaccttccgt
     3241 ggaaagccca tcataaaaac acctatttcc ttggcaacgc caattccgtt cactatgatg
     3301 caccgagaac agaacagcct cataaatgag tcaaccccct tcacaggccc gacggcttat
     3361 cgacctccga aagcacccgc ggctctatac accgtcgggc gctctctttt catttaagcg
     3421 tctcgtggtt ccggttcaat tcgaaggcaa tgcggagggc gaagggacac tcattgattt
     3481 gtccctcgga gggtgtcgct tgttaagcga cacgccgctt gaaatcggcg agctgtacaa
     3541 cctgatcctg caggtctcca aggagagcag ccccgtcatc atagaggcag ctgcggtgcg
     3601 atggacacag gccaacacct atgggctcaa gtttctctct gtgcaatcag tcgatgaatc
     3661 tcaccttcga gacc
//
LOCUS       DATDNW010000076         6962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_622307, whole genome shotgun sequence.
ACCESSION   DATDNW010000076 DATDNW010000000
VERSION     DATDNW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622307"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VI359_01955"
     CDS             complement(<1..321)
                     /locus_tag="VI359_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973046.1"
                     /translation="MQQTVVRILATALVASCPHFAIAGTGEPSASVESSAASTLTKPS
                     GSLLKLDDAIRAGLATHPLVERARHSLLIAKALTRQTQGERYPWLEASVAESSGSLRI
                     VTTDG"
     gene            complement(374..1573)
                     /locus_tag="VI359_01960"
     CDS             complement(374..1573)
                     /locus_tag="VI359_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6973047.1"
                     /translation="MPLQFTLDERTPWGNMTLTSRFKIILTVAGGLFLLAVILMIIQG
                     QEPTVSSPDTGKSSESPSGEAQQVQVLKVQRRDINQTLTLPANISPWYQATLYGKVSG
                     YVKWIGVDKGDTVKQGQLLAVMDAPEIEDQYKQAEADYAIKKVTYERYLSVWEDNHDI
                     IAKQDVDVAKAAAESAKHQRDSRRTLLDYTKVYAPFSGVITARFADPGALIQAATGSA
                     TQAAPLFTIMDMDMVRIYVNVPQEAAMLAKAGLPATLTARELSGEELRASITRTTGAL
                     DLATRTLLVEIDLPNKDHRLQPGMFVSASLHLQQHPNALAIPPAAVVPGKSGKDTSVF
                     VVDLGKAKLVPVKTGIDDGLWLEVVEGLTGDEEVVVVGKGGLSDGQAVQAAAYNLPNG
                     APAKQKM"
     gene            complement(1584..4847)
                     /locus_tag="VI359_01965"
     CDS             complement(1584..4847)
                     /locus_tag="VI359_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6973048.1"
                     /translation="MVRASLKNPFAVIAISLIVIIIGVVSYQKMVVDIFPEINMPVVA
                     VATFYKGMGPSEVEGAITLRLEQAFLQASYIDHIESRSLPGVSLIKIYFHSSYDVNAG
                     LAEITSLTYANLRYLPQGIFPPIIIKFGAASLPIAVQTTSSETMTEKEVRDLAYFNVR
                     PQLGNVPGVAFPTTFGGTVRQVTVFLDPERMLARGVSTHEIVNSVNMQSVLLPAGDVK
                     IGDFDYNVYTNSMIKVVDQMNDIPIKVVNGVPVFLRDVGKAVDSTMIQTNVVRINGHR
                     AVYLPIMKQAGANTIAVIDGIKDVLPRLIGIPKDLTVKLLFDQSLYIRQAIKTLEHEG
                     LLGGGLACLMVLVFLGSLRSTLIVALAIPLSVTAAFIGLYFTGHSVNIMTLGGLALAV
                     GRLVDDAIVVVENFHRHLEMGKSASQAAADAVTEVALPMLIITITVFLVFLPIAFFTG
                     VIKFLFVPLALAVAYAMMASYIVALTVAPVSMARLMRGHTSHGQGGPPDAHARGLTGL
                     WNRLNLFDPFVERYVRVLRWCLAHKAIVVVTVTLVFVGSLFMAPLMATEFFPKVDAGS
                     FILNVSSPEGTRIEKTEAIVAKIEDLIRQHIPAAELDQIVSNVGLPQGWMVLYTPVNG
                     PHQAFVLVSLKRDHGVRTDDIIVKLRETLRRELPGLKFSFQTGGIVSDVINFGLPAPI
                     DIKVSGPKMGDLAATASKIRDLVAEIPGTADVQVRQGMDYPEIHLDINRDKASYVGLN
                     EYQIVTDLVTGLSSNVTLNPGYWIDPRTNNAYFVVTQFPEQILVEFEDFLNMPLVGIH
                     VEHPMATSVGGTRLRGSSLVLGQTPFPERPHLPGVMSNGGSPILLRDLVEVVRKVGPE
                     TVDHFNLQRTMDVLVSVPGNDLGRVATQLEQAVAKLEIPKDVMLTFKGEVDSMRAAIA
                     GFAGTLPLAMVLIYLVMVGLFRSWLDPFVVMFAVPLGFIGVIWMLLLTHTSVNVESLI
                     GTLMMIGIVVSNSVLLVDFANNKLREGMALEEAVVEAGRLRIRPILMTSLATVIGLAP
                     MALGIGEGSESNMPLARAVIGGLTVSTFMTLLFIPVLHAIARRRSAASMAGGDAEIQ"
     gene            complement(4866..5171)
                     /locus_tag="VI359_01970"
     CDS             complement(4866..5171)
                     /locus_tag="VI359_01970"
                     /inference="COORDINATES: protein motif:HMM:NF045581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG0541 family transporter-associated protein"
                     /protein_id="HEY6973049.1"
                     /translation="MTMLLLIFRRSLDHDLHQLLDQLDAKAFTEAPKILGMGEAGATF
                     NSFDWPGHNSMIIAALEDEQADKVVKRLREFRDDLARRQKGAKIPMRVFAHPCTRLI"
     gene            complement(5168..5704)
                     /locus_tag="VI359_01975"
     CDS             complement(5168..5704)
                     /locus_tag="VI359_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973050.1"
                     /translation="MSMSQLNNVLPIAPASQFAQAARILILEDSPGEAELFCEALVLA
                     WRSAGCGSEASRPGIEVQHTAQDALNSLKKRAEPETGNLPELVVLDLDLPGRTSLTFL
                     RELRQDRRLTRLPVIAMAWCDEQPIVRALDGLGVAGYLVKPLRFADLLIMVGNICRTW
                     LPGDRRRELCLLDRERNI"
     gene            6525..6899
                     /locus_tag="VI359_01980"
     CDS             6525..6899
                     /locus_tag="VI359_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973051.1"
                     /translation="MDTTRGRRCNQAGLLVLFLMVAGFSCARTPVPLDLTSPDPSQDQ
                     RKIAGYHSREATVFRLKADEFSQRVMVYERLFGPESEWVEGARLLVQFYEDAAKEQER
                     LAGWHLDIANNGRSQSMRPTLP"
BASE COUNT         1614 a         1995 c         1867 g         1486 t
ORIGIN      
        1 cccgtcggtc gtcacgatgc ggagggaccc gctgctttcg gctacggagg cttcaagcca
       61 gggataccgt tctccttgtg tctggcgcgt cagggccttc gcgatgagta aactgtgccg
      121 cgctcgctca accagcggat gagtggcaag acctgctctg atggcgtcgt caagtttcag
      181 caaagagccg gatggtttcg tgagggtgct tgccgcagaa ctttccaccg atgccgatgg
      241 ctctcccgtc ccggcgattg cgaagtgggg acagctcgcg acgagtgccg tggcaagaat
      301 tctgacgaca gtttgctgca tggatcgctg ccctcgcgag tgtttgagca attcccttct
      361 ataaaggaag aggctacatt ttctgtttgg ccggtgcgcc gttgggaaga ttgtatgccg
      421 ccgcttgaac ggcttggcca tcggataacc cgcccttgcc gaccacgaca acctcttcat
      481 caccggtcag tccttccacc acttccaacc aaaggccgtc atcgatgcca gtcttcaccg
      541 gtacgagttt ggccttgccg agatcgacca caaacactga tgtgtccttc ccgctcttgc
      601 cgggaacaac ggcagccggc ggaatcgcca acgcgttcgg atgttgctgc aaatggagcg
      661 acgcgctcac gaacatcccc ggttgtaacc gatgatcctt gttcggcaga tcgatctcca
      721 ccaggagggt tctggtggcg agatctaggg caccggtcgt ccgtgtaatg gatgcccgca
      781 actcttctcc tgacaattcc cgagcagtta gtgtcgcggg aagtcccgct ttcgccagca
      841 tcgccgcttc ttgaggcacg ttcacgtaaa tccgcaccat gtccatatcc atgatggtga
      901 acaacggagc ggcctgagtc gccgagccgg ttgcggcctg gatcagcgcg cccgggtcgg
      961 caaatcgagc cgtgatcaca cctgaaaatg gggcatagac tttcgtatag tccaacaagg
     1021 tgcggcggct gtcccgctga tgtttggcag attcggctgc ggctttggcc acgtcgacat
     1081 cctgtttcgc aatgatgtca tgattgtctt cccatacgct caagtaccgt tcataggtca
     1141 ctttcttgat ggcataatca gcctcggcct gcttgtattg atcttcgatc tccggcgcgt
     1201 ccatgacggc caataattgg ccctgcttga cggtatcgcc cttgtcgacc ccgatccact
     1261 taacatagcc tgataccttc ccatacagcg ttgcttgata ccagggagaa atgttggctg
     1321 gcagggtcag ggtctgattg atgtcgcgcc gctgcacttt taacacctgg acttgctgcg
     1381 cttcgcctga cggcgactcc gagctcttgc ctgtatcggg cgaagacacc gttggttcct
     1441 ggccctggat gatcatcaag atcaccgcga gaagaaagag ccctccggca accgtcagga
     1501 tgattttgaa ccgagatgtg agtgtcatat tcccccaggg ggtccgctca tcgagagtaa
     1561 attgcagtgg catcgttcct cccttactga atctccgcat caccgccggc catactggct
     1621 gccgatcgcc gacgggcgat agcgtggagg accggaatga agagcaaggt cataaatgtg
     1681 gagacggtca atcctccgat caccgcgcgc gcgagcggca tgttgctttc gcttccttcc
     1741 ccgataccca gggccatcgg agccaacccg atcacagtcg cgagggacgt catcagaatg
     1801 ggtctaatcc gcagtcgtcc ggcttctaca accgcctcct cgagtgccat cccctcgcgc
     1861 aacttgttat tggcgaaatc caccaagagc acgctgttgg agacaacgat cccgatcatc
     1921 atcaaggtgc cgatcaacga ttccacattg acagaggtat gggtcagaag caacatccag
     1981 atcacaccaa taaatcccaa cgggacggca aacatgacga cgaaagggtc gagccaggac
     2041 cgaaagagac cgaccatcac gagatagatc agcaccatcg ccagcggaag agtcccagca
     2101 aacccagcta tcgccgcccg catgctgtcc acttctcctt tgaaggtcag catcacatcc
     2161 ttggggatct ccagtttcgc gacagcctgc tcgagctgag tcgcaacgcg gcccagatcg
     2221 ttacccggca cactcaccag cacatccatg gtccgttgca gattgaagtg atcgacggtt
     2281 tccgggccga cctttcgcac gacttccacc aggtctcgca ataatattgg actgccgccg
     2341 tttgacatga caccaggtaa gtgcggtcgt tccggaaagg gggtctgccc gagcaccaac
     2401 gaactgcccc tgagcctggt cccccccaca ctcgtggcca tcggatgttc gacgtgaatt
     2461 ccaacgagcg gcatgttcag aaaatcttca aactccacca agatctgctc ggggaattgg
     2521 gtcacaacga agtaggcatt gttggtcctg ggatcaatcc aatagccggg attcaacgtc
     2581 acgtttgagg acagtcctgt gaccaaatct gtcacgatct gatactcatt caacccaaca
     2641 taggaggctt tgtctcgatt gatatcgagg tgaatctccg ggtagtccat tccctgccga
     2701 acctgtacat cagcagtacc gggaatctct gccacaagat cacgaatttt actggctgtc
     2761 gcagccagat cccccatctt cggaccactc actttgatat cgatcggcgc gggcaagccg
     2821 aaattaatca cgtcgctgac gatcccgcct gtttgaaagg agaatttcaa tccgggcaac
     2881 tcgcgacgga gggtctcccg caacttcacg atgatgtcat cggtgcgcac cccatggtca
     2941 cgcttcaaac tcacgagcac aaaggcctga tgggggccat tcaccggtgt ataaagcacc
     3001 atccagccct gtgggagtcc cacgttcgaa acgatctggt ccaattcggc tgcagggatg
     3061 tgttgtcgaa taagatcttc gatctttgcc acgatggctt cggttttttc gattcgggta
     3121 ccttctggag aagagacatt gagaatgaac gacccagcat cgaccttggg aaagaactcc
     3181 gtggccatca agggtgccat aaaaagggac ccaacgaaga ccagtgtcac ggtaacgact
     3241 acgatcgcct tgtgtgcaag gcaccaccgc aatacacgca cataccgctc cacaaacgga
     3301 tcgaaaagat tcaaacggtt ccacaacccc gtcagccctc ttgcatgtgc atccggcggt
     3361 cctccctgtc cgtgggatgt gtggccgcgc atcagccgtg ccatcgagac cggcgccacc
     3421 gtgagcgcga cgatatagga agccatcatc gcataggcga cggcgagcgc caacggtacg
     3481 aatagaaact tgatcacgcc tgtgaagaat gcgatcggca aaaagaccag aaacaccgtg
     3541 atggtgatga tgagcatcgg aagggccact tcggtgacgg catccgcagc cgcttgcgag
     3601 gctgatttgc ccatttccag atggcgatgg aagttttcaa ccacgacaat tgcgtcatca
     3661 accagccgtc cgaccgccag ggccagcccg ccgagcgtca tgatgttcac cgaatggccg
     3721 gtgaaataca gtccaatgaa ggcggcggta acggagagag gaatggccaa cgcgacgatg
     3781 agcgtcgagc gcaggctccc aagaaacacc aggaccatta aacaagcgag ccctcctccg
     3841 aggagtcctt cgtgctccag agtcttgatg gcctgacgga tatagagcga ttgatcgaac
     3901 agcagcttga ccgtcagatc tttgggaata ccgatcagtc tggggagaac gtctttgatt
     3961 ccgtcgatga cggcaatggt attcgctcct gcctgcttca taatgggcag atagacggcc
     4021 cggtggccgt tgatccgcac gacgttggtc tggatcatcg tagaatcgac cgccttccca
     4081 acgtctcgca ggaagaccgg cacaccattg acgaccttga tcgggatgtc attcatctgg
     4141 tccacaacct tgatcatgct gttcgtgtag acgttgtaat caaagtcgcc gatcttcacg
     4201 tcaccggccg gcaggaggac gctctgcata ttgacggaat tcacgatctc atgagtggag
     4261 acaccccgcg ccagcatccg ttcgggatca aggaagaccg tgacctgccg aacagtcccg
     4321 ccgaacgtgg tcggaaacgc gacgcccggc acgttaccaa gttggggccg gacgttgaag
     4381 tacgccagat cccgcacttc cttttccgtc atcgtctcgc tgctagtcgt ctgcacggcg
     4441 attgggagac ttgctgctcc gaacttgatg atgatcggag ggaatatccc ttgtggaaga
     4501 taccgcagat tcgcataggt gagactggtg atctctgcca agccggcgtt cacatcatag
     4561 gaggaatgga aatagatctt gatgagactc acacccggca atgaacggga ttcgatatgg
     4621 tcgatgtatg aggcctggag aaaggcttgt tccaaacgaa gcgtaattgc cccttcgact
     4681 tccgatggcc ccatgccttt atagaacgtc gccacagcca cgaccggcat gttgatctcc
     4741 ggaaagatgt ccacgaccat cttctggtac gacaccacgc cgatgatgat gacaatcaaa
     4801 ctgatcgcga tgacggcgaa aggatttttg agcgacgcgc gaaccatgag tagcggtgcc
     4861 ctcctttaga tcagtcgtgt acagggatgt gcaaacactc gcatcgggat ctttgctccc
     4921 ttctgccggc gggcgagatc gtcacgaaat tcccgcaatc gcttcacaac cttgtcggcc
     4981 tgctcgtcct ctaacgccgc gatgatcatc gagttatgcc ccggccagtc aaatgagttg
     5041 aacgttgctc ccgcctcccc catgccgaga atcttgggcg cttcggtaaa ggctttcgca
     5101 tcaagttggt ccaagagttg atgaaggtca tgatccaacg atcggcggaa gatcagaaga
     5161 agcatcgtca tatattgcgc tccctatcta acaggcagag ctcccgacgt cgatccccgg
     5221 gaagccaggt tcggcagatg ttgcccacca tgatgagtag atcagcaaaa cgcagtggct
     5281 tgacgagata tccagccacg ccgagcccat cgagggcacg cacgatcggt tgttcatcgc
     5341 accaggccat ggcgatcacc ggaagacgtg tcagacggcg atcctgtcgt agttcacgca
     5401 aaaatgtcag gctcgtgcgc ccgggaaggt cgagatcgag cacgacaagt tcaggaagat
     5461 tacctgtctc cggctcggct cgcttcttca gagaattcag tgcatcctga gcggtatgct
     5521 gcacctcaat gccaggtcgc gatgcctcac tgccgcatcc ggccgacctc cacgcgagaa
     5581 ccagggcctc gcaaaacaac tctgcttccc cgggactatc ttccaagatg aggattcgcg
     5641 ccgcctgcgc aaactgagac gctggtgcta ttggaagcac gttgttcagt tgagacatgc
     5701 tcatgtggtc gtattggaaa caggctgcgg gaatcgagag gaagagagga cgacttgcca
     5761 ggattctcgt gacagaatgg cacaactgcg acaaaacgac acaggcgtgt aactccttcc
     5821 taaaatcgac gatgggctga cgatcatgtt atcactgcga ccgactgtga attctctcgt
     5881 ccgatctacg acagatagag ttatcgtgcc cactccgaac cttgctacga gggaggtcag
     5941 caatcgcagt gccaggtata tcgcagaggc aatcaacatt gtgagagcaa ggaaccttca
     6001 gctcataatc aatctgagcg gagtcgcaca tgccgcacag tgcgaatgag cgaccgcgag
     6061 ctgacaacag tcattataaa tagatcccca tccgtctcct tcgtcgagag gtttggtaag
     6121 caggccggta atttttgata tgccggactc aaccaacgaa actggaggtg aggactaaga
     6181 tttgcacagc tatgaaagtg aggacgtgct gggtcgtctc tcaatgtccg cagacgaccc
     6241 ttcggacaag gccacgatga aacccataga catgtccggg ccagaatcag agattgagat
     6301 cagaatccgt acccttcaac ccatacgacg acggttgatg ttttccgaac tcgccgaggc
     6361 ttttcccgag tgcacctggc agattctgtt caccgcgctc agtcgattga gccaacgaaa
     6421 acacgtcgag cttgtcgcac accggtggga ctatgaagtg atttttctgc gcactggtcc
     6481 ttccgaacaa actacgtgca gaccctcgaa tgcgagcgaa cggtatggat acaacgagag
     6541 ggcgccggtg taaccaagct ggtctgcttg tcctgttctt gatggtggca ggcttcagct
     6601 gtgctcgaac acctgtccca ctcgacctta cctccccaga tccctctcaa gatcaacgga
     6661 aaatcgccgg atatcatagt cgagaagcaa ccgtgtttcg gttaaaagca gatgagttct
     6721 cacagcgggt catggtctac gaacgcctgt ttggacccga gtccgaatgg gtcgaaggcg
     6781 cgcgcctcct cgtccagttc tacgaagatg cggcaaaaga gcaggaacgc cttgcgggtt
     6841 ggcatctgga cattgccaat aatggccgtt cccagtctat gaggccaact ttaccttaac
     6901 aacggtcatc atggatgacg tatggaggtc tctatgtctc gtcatatcac acacgctttg
     6961 at
//
LOCUS       DATDNW010000077         4349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1169328, whole genome shotgun sequence.
ACCESSION   DATDNW010000077 DATDNW010000000
VERSION     DATDNW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169328"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1336
                     /locus_tag="VI359_01985"
     CDS             215..1336
                     /locus_tag="VI359_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973052.1"
                     /translation="MLHAGIRMRFASAIASIMAIGAIAVVPATEASCGAVSCFVTIGS
                     QQQVAQEGLLTINGIYNYTPMRLIPGTSGIIPGIDTTQRQLILDHHQETRTITQTATI
                     DLNYGITERLGLEITIPYMWRTHHHIDGLGEDGPNGEGQSTTFSSDGLGDIRVGLKYN
                     VLPTLRSMVVLGFGVYLPTGVTNANDSTGTLMEPTTQLGRKQFGLNPTIYQTYELIPH
                     RLNQFSFASYRHTFKNSNGYQFGDQWDFNGGFNLVTVPWLVLTTQFNYRYMVHDTFQS
                     SLLRSATPADAPDFPGEPVVIDPNVRDRRVPTTGSTFLAFTPGFQLSLAEMIDSPWTR
                     ATSVYFYSSVPIARDSNNSLAQGTSYIFGLSRTFRISNP"
     gene            1370..1924
                     /locus_tag="VI359_01990"
     CDS             1370..1924
                     /locus_tag="VI359_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY6973053.1"
                     /translation="MRSGRINSVLVMIVLLIVMVGWPAFDGWSMGSRVPAVGMEVEDF
                     QLTDLDDKPHSLSQYRGKVVLLNFWATWCKPCTTEMPAMQASFDKLREKGFVVLAINE
                     LEDDVKVREHIKTYGHTFTVLMDRDNKVANQFGVFGLPVSVFIDQQGRVQEYIKGGLL
                     TEQIINDVVARIQKQEPVKAASLR"
     gene            2118..3197
                     /locus_tag="VI359_01995"
     CDS             2118..3197
                     /locus_tag="VI359_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973054.1"
                     /translation="MGGTTGHRSVRHVILVFSVLVLSFFPISISLPASPIQQDPAQRQ
                     IERGLSLLHQGELKGATEAAKKAIELNASSAEAYHLLGLIYFKERKPAEAAEAFSRAV
                     KLKPAYPEALNDLADVYLAQGKAAEAERTLTRAIEVDPQHVDSYLDLAKLYEQRHDVV
                     AATKTYQSLLSVQPNQTKALFSLASLYDTKGDSKSAKDTLSRLVKLDPTHADAWYLNG
                     RIAERRNDLVEAAYSYKQAIIAKPDFVDAHYNLAFIYQSQGQPREAEREFLEVIRYRP
                     EYAEAHMNLGVIYTSLNRLDEAEQAYERAVALKPNYAEGHYNLGVFYELHRKDLPRAL
                     AQYHKYRDLGGRDDRVERIIGSGGR"
     gene            complement(3284..3829)
                     /locus_tag="VI359_02000"
     CDS             complement(3284..3829)
                     /locus_tag="VI359_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEY6973055.1"
                     /translation="MSGPWRIMLVASCLTAGACATTYDASQDTTGGAPQITFVQLKAA
                     PDSFKGQSVVFGGEVLTAKRLKEGTRIEILQLPLDQSSRPGINLIQSQGRFLAIHRDF
                     LDPATLPYGTRVTVTGDVSGSVTMPLDEVDYIYPVIDIKTLRVWSDIDDVAPRIRPYY
                     YGPWPYWGPYWGPYWRPLPYW"
     gene            3913..>4349
                     /locus_tag="VI359_02005"
     CDS             3913..>4349
                     /locus_tag="VI359_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HEY6973056.1"
                     /translation="MGKSHRVRRMVSAIVLSVIGLVGMGLSQAAVPDDLKGKFELLKD
                     EASLHERGKVKLFEFADFYCPHCHHFEEAGLPMLQKEFGNKLDATMVGFPVFQNKLPT
                     PFDMYEQAKMMGKGKEMKQVLFRTIHKDKMTGVLDRSIREVLI"
BASE COUNT         1012 a         1128 c         1236 g          973 t
ORIGIN      
        1 gttctcagca ccgcgcttga aagcccaacc tcccgcggtc ctccttctat ctccatcgtt
       61 tagtcgaatc tgctcgcaac attcgagtgc ggaactgaac cccgcgttca ggtttctgca
      121 tcgtagctgc tcagattctt caccatcatg agggagattt cacacatgcg gtccgttcaa
      181 gacgtatcgc aaaaagaagg agtagaaacg agacatgcta cacgcgggca ttcgtatgag
      241 gttcgcctcg gccattgcct cgatcatggc aataggtgcg atcgcagtgg ttcccgcgac
      301 cgaagcatcc tgtggggcgg tgtcgtgctt tgtgacgatc ggctcccagc aacaggtcgc
      361 acaggagggg ctgctgacca tcaacggaat ttacaattac acgccgatgc gattgatacc
      421 tggaacgtct ggcatcattc cgggtatcga cacgactcag cggcagctga ttctcgacca
      481 ccatcaggag acccggacca tcactcagac ggcaaccatt gacctcaatt atggcatcac
      541 ggaacggttg gggctggaaa ttacgattcc ctacatgtgg cggacgcatc atcacatcga
      601 tgggctagga gaagacggtc cgaatggcga aggacagtcc accacgttct catccgacgg
      661 actcggggat atacgggtag gcctgaaata caatgtgctg cctacccttc gcagcatggt
      721 cgtgctcgga tttggagtat atctgccgac tggtgtcacc aatgcgaatg acagtactgg
      781 aacgttgatg gaaccaacta cccagctcgg acggaagcag ttcggcttga atccgacgat
      841 ctatcaaaca tacgaattga tccctcatcg gttgaaccag ttttcctttg ccagctatcg
      901 gcacaccttc aagaacagca acgggtatca attcggggat cagtgggatt tcaatggtgg
      961 gttcaatctc gtaacggttc cgtggttggt acttaccacg cagttcaatt atcgatatat
     1021 ggttcacgat acgttccaaa gttcgctctt acgctccgcg acccctgcgg atgcgccaga
     1081 tttccctggt gaaccggttg tgattgaccc aaatgttcga gaccgccggg tgcccaccac
     1141 aggctcgacg tttcttgcat tcacaccagg ctttcaactc agcttggccg agatgatcga
     1201 ctcaccctgg acgagggcga cctcagtata tttctattcc tcggtaccta tcgcgcggga
     1261 ttctaacaac agtttggcgc aaggaaccag ttacatcttt ggactttcac gcacgttcag
     1321 aatatcgaac ccataaacaa gtggagcggt tgatgcgaag gaggctgaca tgagatcggg
     1381 aagaatcaac agcgtattag tgatgatcgt gttgctcatc gtcatggtgg gatggccagc
     1441 cttcgatggc tggagcatgg gctcgcgcgt tcccgccgtt ggaatggagg tggaggattt
     1501 ccagctgacg gacctggacg acaagccgca cagcctcagt cagtaccgtg gcaaggttgt
     1561 cctgctgaac ttctgggcga cctggtgcaa accctgcaca acggaaatgc cggcgatgca
     1621 agccagcttc gataagctcc gcgaaaaagg gtttgtcgtg ctcgccatca acgaattgga
     1681 agacgatgtc aaagtgcgcg agcacatcaa gacctatggc catacgttca cagtcctgat
     1741 ggatcgggac aacaaggtgg ccaaccagtt cggcgtgttt ggccttccgg tgagcgtgtt
     1801 catcgatcaa caggggcgtg tccaggaata catcaagggg gggctgctga ccgagcagat
     1861 cattaacgac gtcgtcgcga gaattcagaa gcaagagccg gtgaaagcgg cctctcttcg
     1921 atagtaaatg atggccagtg tgattcgaca attctgaaca gagaggtgat cagattcgcc
     1981 atcgattcgt tcgcgcgtgc gattggcgat caccgtcgga tgatgtcccc cagtgcagtt
     2041 cagtaggcgc tcattattga tttcgccgtc tcctgccgtt tatagtccgg tcgggcggtt
     2101 tcattgtgag ggtaagtatg gggggaacga caggacatcg atctgtcagg catgtcattc
     2161 tggttttttc cgtcctggtc ctctcgttct ttcccatctc tatttctctc cccgcgtctc
     2221 ccatccaaca ggatccggct caacggcaga tcgagcgggg tctatcgctt ttgcaccagg
     2281 gagagttgaa aggcgcgacc gaagcggcca agaaggccat cgagttgaac gcctcgtctg
     2341 ctgaggcgta tcacctgctg ggcctcattt acttcaaaga acgaaaaccg gctgaagcgg
     2401 ccgaggcttt ttcgcgcgcc gtgaaactga agccggcgta tcccgaggcg ttgaacgatc
     2461 tcgccgatgt gtacctggca caggggaaag cggccgaggc ggagcgaacg ctgacccgcg
     2521 cgatcgaagt tgatccccag cacgtggact cctatctcga cctggccaag ttgtatgagc
     2581 aacggcatga cgtggtcgct gcgacgaaaa cctatcaatc gcttttgtcc gtacagccga
     2641 atcagaccaa agcgctgttc agcctcgcca gcctctacga cacgaaaggc gacagcaagt
     2701 cagcgaaaga cacgttaagc cggttggtga agcttgatcc gacccatgcc gatgcgtggt
     2761 acttgaacgg acggatcgcc gagaggcgca atgatctggt cgaggccgcc tattcctaca
     2821 aacaagcgat tatagcgaaa ccggatttcg tggacgcaca ttacaacctg gcctttatct
     2881 atcaaagtca gggtcagccg cgggaggccg aacgggaatt tctggaagtg atccggtacc
     2941 gccccgaata tgctgaagct catatgaacc tcggcgtgat ttatacgagc ctcaatcgat
     3001 tggatgaggc ggagcaggcc tatgaacggg ccgtggcact caagccgaat tatgcggaag
     3061 ggcattacaa tcttggagtg ttttacgaat tacaccgcaa agatcttccc cgtgccttgg
     3121 cccaatacca taaatatcgc gacttgggtg ggcgagacga tcgagtggag cggattatcg
     3181 gttcaggggg gcgatagatc ggaaggaaaa ggctgaggcc tccgtcgagg gattgatgtg
     3241 agcctggagc gctcgatctg cgcctctggt atccacgtct cgttcaccaa taaggcaagg
     3301 ggcgccagta gggaccccaa tagggccccc aataaggcca aggaccataa tagtagggcc
     3361 gtatacgagg ggcgacgtcg tcaatatccg accatacccg tagcgtcttg atgtcaatca
     3421 ccggataaat ataatcgact tcatccaacg gcatggtgac cgatccgctc acgtcgcctg
     3481 tcacggtgac tctggtgccg taaggcagcg tcgcaggatc gagaaagtca cgatggatgg
     3541 ctaaaaaacg tccttgtgat tggatcaggt ttattccggg acgggacgat tgatcgagcg
     3601 gcagttggag aatttcgatc ctggttccct ctttcaatct cttggctgtg aggacttctc
     3661 ctccaaagac tacggattgg cctttgaagg aatccggggc tgccttgagt tgcacaaagg
     3721 tgatctgagg cgcgcctcct gtggtgtcct ggcttgcgtc gtatgtggtg gcacatgcgc
     3781 cggctgtgag gcaggaggcc acgagcatga tccgccatgg tccggacatg gctggcatta
     3841 tatcaaacag cacgcgcgtg atttctttcg atataatgag accggtttga ttaccgagga
     3901 aaggagtgcc gcatggggaa gagtcatcgt gtccgccgta tggtttccgc aatcgtccta
     3961 tctgtgatcg gtttggtggg aatggggctc tcacaggcgg ctgtcccaga cgatctcaag
     4021 ggaaagttcg agctgttgaa ggatgaggcc tcgttgcatg agcgaggcaa ggtcaagcta
     4081 ttcgagttcg ccgattttta ttgtccacac tgccaccact tcgaagaggc cggtctcccg
     4141 atgctccaga aggaattcgg aaacaaactg gatgcgacga tggtcgggtt tcccgtgttt
     4201 cagaataaac tgccgactcc tttcgacatg tatgagcagg cgaagatgat ggggaaaggg
     4261 aaggaaatga agcaggttct cttccgcacg atccacaaag acaagatgac tggcgtgttg
     4321 gaccgttcga tacgggaagt cctgatcaa
//
LOCUS       DATDNW010000078         5389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_148932, whole genome shotgun sequence.
ACCESSION   DATDNW010000078 DATDNW010000000
VERSION     DATDNW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148932"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VI359_02010"
     CDS             complement(<1..520)
                     /locus_tag="VI359_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973057.1"
                     /translation="MAMDWVVLIGVLVLLLVGGTAAFYLHAFVARTRKEFRDTQPNLR
                     ISNLSAMNAGNVLTLSPEIENMGRGVAYDCVLHLGGWEGNFAVKKVYPRDSRYQKHAA
                     SIVLGPDAPLRGRPMTNGYLRVSYRDRWGLKYECWYPVTQIKSGAIPLYNIQIDLEHP
                     ELTEPNPSFWEMR"
     gene            complement(679..1719)
                     /locus_tag="VI359_02015"
     CDS             complement(679..1719)
                     /locus_tag="VI359_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973058.1"
                     /translation="MNVPRKQAAFLREAIEQWKHDGLLADAQAAQLAGSILVQPFDWR
                     RLAKYSFWIALFSIVSSVSAALSDQFLIDLLEQLFNAPAAAKCAGLALMAAGLYWWGV
                     RRRALDPTQVYRNEAILFLGVLATAGAIYQLGMALDTGSGHFSILLLLSFLVYAILGV
                     AVTSNLIWVFALASLGGWMGTETGYMSGWGAYYLGMNYPLRFVLFGGVLTVCALALET
                     HPKAQRFFRSTLTMGLLYLFIALWIMSIFGNYGDIGAWERAKQIELFHWSLLFGLVAC
                     GAIYHGLKYDNGVTKGFGLTFLFINLYTCFFELFWNSLHTAIFFALLGLSFWYLGTKA
                     EKIWNLGRNPAA"
     gene            complement(1864..3060)
                     /locus_tag="VI359_02020"
     CDS             complement(1864..3060)
                     /locus_tag="VI359_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY6973059.1"
                     /translation="MKNQVTILVVDDSSDDITFFTHLLRRDGYSTITATSGQEALDKI
                     ESDHPHLVLLDILLPHMNGYEVCRMIRENPRTALLPVIMVTAVTKERILGIEAGADDF
                     LTKPVNREELLARVRSLLRIKSLHDAVEGHVGELADWNRKLEARLEQEAKLAEVARVL
                     GDISHEVKNLLMPIVTGTDLLQDELKEIFALMPRKGADKLRASEKQCEEIGEMLLRAA
                     NRLHEHVQEIADCVKNLSAPPAFVTCRIATVVDGVVKTLGVLAEGKGISLQVENLSNL
                     PSIQADERRMFNVFYNLINNALAEMPSGGFITLRGDHVADAKMICISVTDTGPGMPPE
                     IRDRLFTEQAISRKVGGTGLGTKIVKDVIDAHGGRISVESELGHGSTFFVSLPIEQAT
                     RVTSSN"
     gene            complement(3223..3657)
                     /locus_tag="VI359_02025"
     CDS             complement(3223..3657)
                     /locus_tag="VI359_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6973060.1"
                     /translation="MKPRITVITVGVDDLERSLKFYRDGLGLPTRGIVGTEFAHGAVA
                     FFDLQNGLKLAIWSRKDLAHEAKVPLGSRSATEFTLGHNVGSKEEVDAVMEQARHAGA
                     VITDAPHDAFWGGYTGSFQDPDGHLWEVVWNPDMEIINELTS"
     gene            4031..4636
                     /locus_tag="VI359_02030"
     CDS             4031..4636
                     /locus_tag="VI359_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973061.1"
                     /translation="MKQRTFLILGIGLASALMTSSIAWADAGARCDGNKAHMMSRHGG
                     HGHGTMTSHLLRHLLKNKQELGLTDEQIAKLRTVALDADRARIRAEAEVKVSERELRA
                     MMWDEKTQLPAIEAKVKEKESFDATVRIIGIRAGRELMGVLTPEQQVKQKALREQYRH
                     QDRRQMRAETDDSTNEASAMEAGINASQVELSELEDGPSAG"
     gene            4719..5348
                     /locus_tag="VI359_02035"
     CDS             4719..5348
                     /locus_tag="VI359_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY6973062.1"
                     /translation="MPGIYLSMADESLTGLARASEDRDLLRRIVRGETDRFADVIRRY
                     QQHVVRIVARRVPADQVEEIVHDVFVRAYFGLAQFSDSVAIDHWLAGIAVRTCYDFWR
                     AKKREELPVSALTDEHHRWMDQVLATQSDDQFRHQVLSQEAAEVLEWALRQLSPENRA
                     VLTLVHLDGHSVREAASLLGWSVVNVKVRAHRARQALRKILSTGLRGST"
BASE COUNT         1303 a         1516 c         1474 g         1096 t
ORIGIN      
        1 tccgcatttc ccagaatgag gggttcggct cggtcagttc aggatgctcg aggtcgattt
       61 gaatgttgta gagcgggatg gctccgcttt tgatttgcgt gacaggatac caacattcat
      121 acttcagccc ccagcgatct cgatagctga cccggagata cccattggtc atgggcctcc
      181 cgcggagcgg cgcgtccggg cccaagacaa tggaggcggc atgtttttga taccgtgagt
      241 cgcgaggata cactttcttg accgcgaaat tcccttccca accacccaga tgcaggacgc
      301 aatcgtacgc gactccacgg cccatgttct cgatttcggg agagagggta agaacattcc
      361 ccgcgttcat ggccgataga tttgaaattc tcagattggg ctgagtatcg cgaaattcct
      421 tcctggttcg ggccacgaac gcatgcagat agaaggcggc ggtaccaccg acgaggagga
      481 gaacgagcac accaatcagc actacccaat ccatggccac atgtgtgtcc aggtgaacga
      541 tccagcgcta gagctaaaac ggagagaggc tacgttctcg agcgagcagt gtcaaccaga
      601 tcaataaaaa gaagtcgaca tcgtaagcag cggcccgccg cccgtttaga catctcagat
      661 tgtctcccga tgtacccatc acgcggcagg gtttcggccg agattccata ttttttcggc
      721 cttcgtcccg agataccaaa aactcaggcc caacagggca aagaaaatcg ccgtgtgaag
      781 ggaattccag aacagttcaa agaagcacgt atagagattg atgaacagaa aggtcaggcc
      841 aaatccctta gtaacgccat tgtcatactt caatccatga tagatggccc cgcaggctac
      901 cagaccgaac aggagcgacc aatggaacag ttcgatctgc tttgcgcgct cccacgcccc
      961 gatgtcgcca taatttccaa agatcgacat gatccagagc gcgatgaaga ggtacaacag
     1021 ccccatggtc agcgtgctgc ggaagaatcg ttgggccttc gggtgcgtct caagggccag
     1081 cgcacagacg gtgagaaccc cgccgaagag gacaaagcgt aacggataat tcattccgag
     1141 atagtaggct ccccatccgg acatatagcc ggtttccgtt cccatccacc cgcccagcga
     1201 ggcaagggca aacacccaaa tgagattcga cgtaaccgct acgccgagaa tcgcgtagac
     1261 aagaaaggac aacagaagca ggatcgagaa atgcccgctg cctgtatcca gtgccatgcc
     1321 cagctgataa atagcgcccg ccgtggccag cacgcctaaa aagagaatcg cttcgttgcg
     1381 atacacctgc gtcggatcca acgcccgcct ccgcacgccc caccaataga gccccgccgc
     1441 catgagtgcc aagccggcgc actttgcggc ggcaggcgca ttgaacaact gctcgagtaa
     1501 gtctatcaga aattgatcgg agagcgccgc gctgaccgac gacacgatcg aaaacagagc
     1561 gatccagaac gaatatttcg ccagccgccg ccaatcgaat ggctgcacga gaatcgaacc
     1621 cgctaactgc gccgcctgcg catccgcgag caagccgtcg tgtttccatt gctcgatcgc
     1681 ctcgcggagg aacgcagctt gtttccgtgg aacattcatc gtcacctcac agaggaagag
     1741 cccgttcact gcgtcagcgg tacaagcacg aaaggctaaa caagcgcgat gcgaaatggg
     1801 gctgagatgc tatgtccgtg gagacaacga tggcgtgaac gtgtgcctga atgaggcgca
     1861 acattaattc gacgaggtga ctcgtgtggc ctgttcgatc gggaggctga cgaagaaagt
     1921 agatccatgc ccgagttcgc tctccacgct aatccgaccg ccatgcgcat cgatgacgtc
     1981 ttttacaatc ttggttccca gccccgtccc tcctaccttt ctacttatag cttgctcggt
     2041 gaacagacgg tcgcgaattt ctggtggcat tccggggccg gtatcggtga cggatatgca
     2101 tatcatcttg gcatccgcca cgtgatcacc acgcagggta atgaagcccc cggacggcat
     2161 ttccgcaagg gcattgttaa tcaaattgta gaagacgttg aacatgcgtc gctcgtcggc
     2221 ttgaatggaa gggagattcg ataggttttc gacctgcagg gagatgcctt ttccttcggc
     2281 aagaacgccc agggttttga cgacaccgtc cactacggtg gcaatacggc aagtcacaaa
     2341 ggcgggcgga gcactgagat tctttacaca atccgcgatc tcctgaacat gttcgtgaag
     2401 gcggttcgca gctcgaagaa gcatctcccc aatctcttca cattgctttt cactggcgcg
     2461 cagcttgtct gcgcccttcc gcggcatcag ggcaaagatc tccttgagct catcttgcag
     2521 cagatccgtt ccggtcacaa tcggcatgag cagattcttg acttcatgag agatatcgcc
     2581 gagcacccga gccacttcag ccaatttcgc ttcctgttcc agtcgggctt ctaactttct
     2641 gttccaatcc gcaagttctc caacatggcc ttccaccgca tcatggagag acttgatccg
     2701 caaaagtgag cgaacgcgag cgagaagttc ctcccggttg accggtttgg tcaggaaatc
     2761 atccgctcca gcttcaatgc ccaggattct ttccttagtc acggcagtca ccataatgac
     2821 gggaaggagg gcggtacgtg gattttcgcg aatcattcga caaacttcat agccgttcat
     2881 gtgtggtaat aagatgtcca gcaataccag gtgaggatga tccgattcga ttttatcgag
     2941 agcttcctgg cccgacgtgg cggtaatcgt ggagtacccg tcccgccgaa gcaaatgcgt
     3001 gaaaaaggta atgtcgtccg acgagtcgtc gacaacgagg atggtgacct gattcttcat
     3061 gaacggctca tcatgagagc atcggcaagg tagtgcttaa tgtctaaaca acgggtggtt
     3121 tcaccagaag tttctcgatg caagctgccg taaactaaga gtatcgttca ttctcatggt
     3181 gtcaaggaaa gagaaagatt cgacttgcgg cccagtagcg tcctacgatg tcagctcgtt
     3241 gataatctcc atatcgggat tccacaccac ttcccacaga tgcccgtctg gatcctggaa
     3301 ggaaccagtg taaccgcccc aaaaggcatc gtgtggcgca tccgtgatca ccgccccggc
     3361 gtgtcgtgct tgttccatta ccgcatccac ttcctcctta ctccctacat tgtgtccgag
     3421 cgtgaactca gttgcgctcc gagaccccaa tggaactttg gcctcatgag ccaaatcttt
     3481 gcgagaccaa atcgcaagct ttaaaccgtt ctggagatcg aagaaggcga ccgccccgtg
     3541 cgcgaactcc gtgccgacaa tgcctcgtgt cggcagaccc agcccgtctc ggtaaaactt
     3601 caacgagcgt tctaaatcat ccactccgac ggtaatgacg gtgattcgag gtttcatagc
     3661 gtctccatgg tctcaccgag tgaatgacag ggacgtaatc cgagaaaggc tagggctaag
     3721 caacggggtc attctacttt ttgaatggca gtccagtcga ttattactca cgctggtccc
     3781 cttcaaacca accccaccgg acaccccaag acgccctctt gcccaagcag gcaacgctct
     3841 cactgtgatt tcaataggtt cccgcgtgcg cgttcccgat tacaggagtc aaccaggtcg
     3901 gccccgactc accccaaaag cctcccccct atctacctgt aaccttttcg aaacttgcca
     3961 cgactaggac acagaaggaa agagaccgat cacagacttg tcccatattg atctgatcaa
     4021 ggaggatccc atgaaacagc gcacgtttct aattctcggc attggattgg caagtgccct
     4081 catgacttcg tcgatcgcat gggccgacgc cggagcccgt tgcgacggca acaaagccca
     4141 tatgatgtcg cgtcatggag gacatggaca tggcactatg acctcccacc tgctccggca
     4201 cctcttgaag aacaagcagg agctgggact cacggatgag cagatcgcca agttgcgcac
     4261 ggtggcgctc gatgcggatc gagcccgtat ccgtgcggaa gctgaggtga aggtcagcga
     4321 acgggaactc cgcgcgatga tgtgggatga gaagacccaa ctgcccgcga tcgaagcgaa
     4381 ggtgaaggaa aaggaatcgt tcgacgccac ggtccggatc atcggcatca gggccggacg
     4441 agagctcatg ggcgtcctga cgccggagca acaggtcaaa cagaaggcct tgcgggagca
     4501 gtaccgacat caggatcggc gtcagatgcg agccgagacc gatgactcca cgaacgaggc
     4561 cagcgcaatg gaagccggca tcaacgcttc acaggtcgaa ttaagtgaac tggaggacgg
     4621 accgtcggcc gggtgacctt gcgacccggc taacactcaa caagacatcg tgcggtgata
     4681 tgatgagcga cggttctctt gtgacgtgta tagtgggtgt gccgggaata tacctgtcaa
     4741 tggccgatga gtcgttaacg ggcctcgcgc gagcatcaga agatagggac cttctccgac
     4801 ggatcgtccg aggagagacc gaccgatttg ctgacgttat cagacggtac caacaacacg
     4861 tagttcgcat cgtcgctcgg cgggtgccgg cggatcaggt cgaagagatc gtccacgatg
     4921 tgttcgtcag agcgtacttc gggctggcgc aattctcgga ctcggtcgca atcgatcact
     4981 ggctcgccgg cattgccgtc cgaacgtgtt acgatttctg gagagcgaaa aagcgcgagg
     5041 agctgccggt cagcgcgttg accgatgaac accatcggtg gatggatcag gtgctggcga
     5101 cacagtcgga tgaccagttc cgccaccagg tgctgagcca agaggcggcg gaggttctgg
     5161 aatgggcact cagacagttg tcaccggaga atcgcgccgt gctgaccttg gtccatctgg
     5221 acggtcattc agtgcgtgag gcggcttccc tgttgggatg gagtgtcgtg aatgtcaaag
     5281 tgcgagcgca tcgtgcccgg caagcgcttc gcaagattct gagcaccggc ttacgaggga
     5341 gtacatgatg aaacattctg atgacgggat ccccaaactc gagcgcgcc
//
LOCUS       DATDNW010000079         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_554421, whole genome shotgun sequence.
ACCESSION   DATDNW010000079 DATDNW010000000
VERSION     DATDNW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554421"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1875
                     /locus_tag="VI359_02040"
     CDS             496..1875
                     /locus_tag="VI359_02040"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HEY6973063.1"
                     /translation="MTEPEFSKPDTDELLRTLHERPHMPLAKWLRAPAHVYYKAFRMT
                     DPPVQRPASRDEFLSLLEAFKVPADASLVREGFGYGVKTATTGDRLIVIWQSHTEYYS
                     YQIWHIPSDRAGGVTFGPMTFPEYRFPITPMGTEVCHLDIVLMAESLPASETLRPQFP
                     GPVLYGSRILDEETVVVASFTPDEHGRERYWVSVGSDGSDTSHLKDIVDAIVRIETYY
                     HLLLMQKPLFSAAIDQAYKFEQVHLKQREIITGHITHADSLTLQRWLNGLTQDLLKTN
                     RLAGKLHFGLSASVPYDKIVHRTLDSLEERTFPPYRPISDYVLGGITGVAEGYQQLLK
                     RIDTLRSGFEGIIFIIRARVDLALQAQNLSLLASVDKTTKNQAILQHTVEGLSVIVIA
                     YYLSGLAAYLFKGLNEIGWLRNPNIATAFFVPVAIGLAFLITSLSRAYLRKKLSAEPP
                     SSVPEKPQA"
     gene            2006..2353
                     /locus_tag="VI359_02045"
     CDS             2006..2353
                     /locus_tag="VI359_02045"
                     /inference="COORDINATES: protein motif:HMM:NF042576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6717 family protein"
                     /protein_id="HEY6973064.1"
                     /translation="MNVVSVIVPYKYEGVWVFDDPAVGLVREPFVAGIDTMIDRLVES
                     IPDAAKGFRLLFSATPFPGYTIKLKWRREEYGGNWYYCNQLGMEGWLCPALFRYFVEA
                     PPELYARVEAKTG"
BASE COUNT          581 a          774 c          788 g          624 t
ORIGIN      
        1 atcagacgct gttcgtcagc gccgatttta ccgagaccgc ctggcgcctc tacgatccgt
       61 tgctcaccgg cgaacgggtc gtgcatccct ataccgccgg cacgtggggc cctcgggaag
      121 ccgacgcgct cgtggaacga aacggacatc aatggcagtt gggatggtaa gagggataag
      181 aaccgggaca tcctcatttt ctaattgcct catccgcgcg cggcatctct gagttccccg
      241 cccagctcga ggccgaatcg tgaccatgtg actgtctgaa cagaagtgtc cactctccgc
      301 acgtcgaatc ttcatggcca gctcttcggc gcactagggg actactcctc gctcggcatg
      361 gaacccccgg atgcgcgaat gtcacgacag gtgatgccga acccgcactc gtccgaagat
      421 cggttgacag ggttgttgag aagaaagtag cctggcaccg tttttctttg atcattcatg
      481 aggcggggtt gtcgcatgac cgagcctgag ttctccaaac cggataccga cgagctcctt
      541 cgaacgctac atgagcggcc ccacatgccg ttggccaagt ggctgcgagc ccctgcgcac
      601 gtgtactaca aagcgtttcg catgaccgac ccgccggtgc agcgaccggc aagtcgggac
      661 gagtttctgt ctctcctgga agccttcaaa gttccggcgg acgcttcgct cgttcgagaa
      721 ggttttggct atggggtgaa gacggcaacg accggcgatc ggctaattgt gatctggcaa
      781 tcccacactg agtactacag ttatcaaatc tggcatattc cctccgaccg tgctgggggt
      841 gtgacgtttg gcccgatgac cttcccggag tatcggtttc cgatcacgcc gatggggacg
      901 gaggtgtgcc atctggatat cgtgctgatg gccgagtcgt tgccggccag tgagacgttg
      961 cggccgcaat ttcccggtcc ggtcctgtac ggcagccgga ttctcgacga ggagaccgtg
     1021 gtggtcgcca gcttcacgcc tgacgaacat ggccgggagc gttattgggt cagcgtgggg
     1081 tcggacgggt ccgacacgtc ccatctcaaa gacatcgtcg atgccattgt ccggattgaa
     1141 acctactacc acttgctgct catgcagaag ccgttgtttt cggctgcgat cgatcaggct
     1201 tacaaattcg agcaggttca tctgaagcag cgagaaatca ttaccgggca tatcacccat
     1261 gccgattcac tgacgctgca acgttggctc aacgggctga cacaagactt attgaaaacc
     1321 aatcggttgg ctgggaagct ccactttgga ctctccgctt ctgttcccta tgacaagatc
     1381 gtgcatagga ccctcgactc tcttgaagaa cgcacgttcc cgccgtaccg accgatttcc
     1441 gactatgttc tcggggggat caccggggtg gcggagggct atcaacagct cctgaagagg
     1501 atcgacaccc tgcgtagcgg gttcgaaggg atcattttca tcatccgcgc ccgggttgac
     1561 ctcgcgctcc aagctcagaa tctttcactc ttggccagcg tcgacaagac gacgaagaat
     1621 caggccatcc ttcagcatac ggtcgaaggc ttgtcagtca ttgtgatcgc ttattatttg
     1681 agcgggcttg cggcgtatct cttcaaagga ctgaacgaga tcgggtggct ccgcaacccg
     1741 aatattgcca ccgccttctt cgtgcccgtt gcgatcgggt tggcctttct catcacgagt
     1801 cttagccgag cgtatctccg caagaagcta tccgctgaac cgccttcttc ggttccagag
     1861 aagcctcagg cgtgaagggt cgaatcgaga tggggaaaca aggccgctct ccttttctgt
     1921 tcgcctcgtc cgcgcacgag aagcagagtg aataccggtg agtttcttgt ccccattgca
     1981 gactcacgtt tgtcgcgttg aaactatgaa cgtcgtctcg gttattgttc catacaagta
     2041 tgaaggcgtc tgggtcttcg atgatcccgc cgttggcctc gtccgagagc cattcgtggc
     2101 cggcatcgac acgatgattg acaggttggt ggaatcgatt cctgatgcgg caaaaggctt
     2161 tcgcctctta ttttctgcga caccgttccc tggctatacg atcaaactga agtggcggcg
     2221 ggaagagtat ggtggtaact ggtattactg caaccaatta ggcatggagg gctggctgtg
     2281 tccggccctt ttcaggtact tcgtggaagc tccgcctgag ctgtacgcca gagtggaagc
     2341 caaaacaggt tgagccgaat cgctgaagag taaagtggag gtgtccagaa ttcgtattgc
     2401 cgatcttcag aggcgtagcg tttcgcgcct ggttgcccgc gcttcttcag aaccaaatcg
     2461 cctcctcggc gcgcgacggc aggtttctct tcagttaacc agttgcgagg ctgggaactc
     2521 aacgaatgtt cccagccctt acgagctcag ggacagaatg gacaggacgg acaacttacg
     2581 gtcacattgc ccagtgcagg acacactctc accagtgcag cgacgactga agcaggtagc
     2641 acagcgagga gccaacgcat ttcatccacc tccttttccg atcggaagtc cgagcaggaa
     2701 gttcctcgat taggcaacag atttcattgg gagtcaatgg cgaggacgaa gaaaagggtc
     2761 gggagta
//
LOCUS       DATDNW010000080         8242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_554427, whole genome shotgun sequence.
ACCESSION   DATDNW010000080 DATDNW010000000
VERSION     DATDNW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554427"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /locus_tag="VI359_02050"
     CDS             complement(<1..2328)
                     /locus_tag="VI359_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6973065.1"
                     /translation="MSITRGQAVPQWFAPIATALASEGGRPCLTGLHGSTAGFALSAL
                     THPQTSVLGTRPWLIVSRSDEAAERLHDDLCFFATLSGLSVDSLAFFPKWETLPYEAT
                     EPHVGLIARRMNALYRARTMPRIRLVTSIDALIHRLLPVETFTGTILQFTRGASLERE
                     ALVGGLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYADPLRVEFLGETIDSLRLFDP
                     ATQKSTAKLDRALVLPAREYLRSENDLDSLSPIPPDAEWRTPDLYARLATLFDFFTER
                     PLLVFDQPAALKTECAALWDKIDDGYLHHSDLKESPSYPSPEKLFQSWDALTDRISGW
                     PILALEPLAETDASWSPVETFSAQTPATAGLSTRGTPFSQTLVILDRIRTECRVVLVA
                     RSRGQVDRLLALLHEHDVPAMEWTPAAWTATSTARSPFYILHGDLSAGFVSTELRLAV
                     LTEEELFAKGARHKPQPKSKAATFLSSLEDLRVGDYVVHVQHGIAKYEGLKRLSVQDF
                     DSDYLILEFAGSDKLYVPLDRLNQVQRYSGAEGHVPRLDRLGGTSWAKTTARVKKDIE
                     EMAHELIDLYANRELVKRQSYGHASTLYHEFEAAFEYEETPDQLKAIDDIVRDMEASR
                     PMDRLVCGDVGYGKTEVAMRAAFKAVEDNRQVAVLVPTTLLAHQHYENFAERFAPFPT
                     RVGLLSRFQSPKDTKTILKDLAAGTIDVVIGTHRLLQKDVVFRNLGLVIIDEEQWFGV
                     KHKERLKQLRTQVDVLTLTATPIPRTLQMAMSSVRD"
     gene            complement(2332..2814)
                     /gene="rfaE2"
                     /locus_tag="VI359_02055"
     CDS             complement(2332..2814)
                     /gene="rfaE2"
                     /locus_tag="VI359_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY6973066.1"
                     /translation="MTSKVVTTEQLVPLLAQARTQGKRIVFTNGCFDLMHVGHTRYLQ
                     AAKALGDLLVVGVNSDASVKSLNKSPDRPIVPESQRAEVLAALGCVDHVVLFAEPDPS
                     RLIRALQPDVLVKGGDWSVETIVGRDIVEGRGGVVKTIPLVPGVSTTTLIHRIRSTQT
                     "
     gene            complement(2863..4017)
                     /locus_tag="VI359_02060"
     CDS             complement(2863..4017)
                     /locus_tag="VI359_02060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY6973067.1"
                     /translation="MHWSRRILFGDVDAMFAAAAVIADPSLAGKPVAVGGQTPRGIIA
                     AASYAVRPYGVRSAMPTIEAFRLCPGLVLIPPDRPLYRRLHDQMRAVTDRLFPLTEWS
                     SIDEFYAETTDLQARHPDPLALGRLVKESIFDATGLRCTVALASGKTVAKIAVDSHKP
                     DGLVAIEPGTEQAFLAPLPIRALPGIGPKTAARLEPLGLRTIGDLLDMRWDGVLRRLF
                     GNHLLEIRELAGGIDREPVCADRESKSLSHETTFEHDTHDRAFIEQTLRGFLRALAHN
                     LRQEGLAAGSCTVKLKDARFTITTKQRRFPGPLNYDPAMWPVVQRTLHELLVPETKYR
                     LAGLALSSLSPTAPGLFDRQRAKAVEAMDTIIAQYGPSVMGLGGVTREDS"
     gene            complement(4042..4707)
                     /locus_tag="VI359_02065"
     CDS             complement(4042..4707)
                     /locus_tag="VI359_02065"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY6973068.1"
                     /translation="MAPSPNLPCSTEKLAAIWADLNTRYFKGILSPIDIVWSTRLTAS
                     VGLFVTTIGPRMSVQRWGMSAPRHREIRLSVPLLTQAFAKTSYGEHEITSTLAHEMIH
                     QWQYDILKRRPNHGPDFMRKMTEMNRDGMLAITTYHSLQDEVVALTRFAWRCRQCGRV
                     YRRQRRTIEPRRHHCGVCRGSLQELRAVTRRQASPNRSEPPGDHRALSATDLQSIQLS
                     FTF"
     gene            complement(4715..5707)
                     /locus_tag="VI359_02070"
     CDS             complement(4715..5707)
                     /locus_tag="VI359_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973069.1"
                     /translation="MAKRPRRTPPHLPTRPLIAPINVSDLKTYPLKKRHSKVRVSDFA
                     KPWKQGGSFSQFYRALPDILAVKTLRAVAKTMAAAHRQGRPVIVGIGAHVVKVGLNPI
                     IVDWMNQSIVSAVAMNGAGIIHDFELALMGHTSEEVDAEIDEGRFGMAEETGRVLNEA
                     IARGARDREGLGEAVGHHINRHQGQFPHRETSILATGARLGIPVTVHVAVGTDIIHMH
                     PSADGAAIGATSLLDFRRLAAVVAGMEGGVYLNLGSAVILPEVFLKTVSLGRNLGHAL
                     TKITTVNMDFLSHYRPLTNVVRRPTQKGGKGYSLIGHHEIMVPLLAAALQEELG"
     gene            complement(5691..6269)
                     /locus_tag="VI359_02075"
     CDS             complement(5691..6269)
                     /locus_tag="VI359_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEY6973070.1"
                     /translation="MKEQAIRAFEESASVKQQFVRDHADRIVQVVKLIATAFREGRKV
                     LLFGNGGSATDAAHIAAEFVGRYHRDRDPLPAIALATDVAAITCIANDYGYDELFARQ
                     IRAHGQKGDIAIAISTSGNSSNVLKGVETARAIGLTTIAWTGGSGGKLAGMVEYPFVV
                     PSKVTARIQESHITLGHVLCELIEEQLLGKTS"
     gene            6381..7637
                     /locus_tag="VI359_02080"
     CDS             6381..7637
                     /locus_tag="VI359_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HEY6973071.1"
                     /translation="MAQDQPVKALVITLTDHPGAAVYSVNRLRPDSLCFVLPESAKSL
                     VESSVQPKIEQMPRRWDWVMLSDAEHFPSCYQTVARSLLSILKAWEVQPGELVVDLTG
                     ATAAMSGSLALVTRPFTSRAVSLVPAAEGREGDAVSVDGHAFIWLQINPWDEAATVSR
                     QEAFDYFNRGAFVAAAALFRLIEMRVSGGQKPLYRAFFDLAEGYDLWERFHYRQAWDK
                     LKTSIKALEMASVWGGPPGVKELLPSMKANAGFLEKLVLDPAEVKDIGVLDLLANVRR
                     RLDVDRDPERAMVALMRTLEAVAQRQLFKQYKVKTWDLQPEQLPQALRETCRTCYLDD
                     VDGKYKLPLQAQFRTLAGLGDQLGQGFLREWPTLKPLLDAASHAVLGHGFEAIKAERV
                     QQLYEVVLKLSGVSESSLPRFPVLNL"
     gene            7702..8109
                     /locus_tag="VI359_02085"
     CDS             7702..8109
                     /locus_tag="VI359_02085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEY6973072.1"
                     /translation="MEIRIYYEDTDCGGIVYYANYLKYFERARTEYLEARGYSVAALM
                     AEGIVFVVVHAEVDYCSPARYGETLVVETVVSDLSRASLTFSHAVRERASGRVIVEGS
                     ARLAVTDGSGKVKRLDKAIVVALESGSGRRSDG"
BASE COUNT         1686 a         2369 c         2494 g         1693 t
ORIGIN      
        1 atcgcgaacg ctcgacatcg ccatttgcaa cgtgcgagga atcggcgtcg cggtcaaggt
       61 caggacgtcc acctgcgtgc ggagctgttt cagtcgctct ttgtgcttca cgccgaacca
      121 ctgctcttca tcgatgatca cgagcccgag gtttctgaag acaacgtcct tttgcagcaa
      181 ccggtgggtt ccgatcacga catcgatcgt gccggctgcc aggtccttga gaatggtttt
      241 tgtgtctttc ggtgattgaa atcgggacag caaccccaca cgagtgggga atggagcgaa
      301 ccgttcggca aagttctcgt aatgttgatg ggcgaggagc gtcgtcggaa caagcaccgc
      361 cacttggcga ttgtcttcca ccgccttgaa ggccgctcgc atcgctacct cggtcttacc
      421 atacccgaca tccccgcaga ccaaccgatc catcggcctg cttgcttcca tgtcgcgaac
      481 aatgtcgtcg atcgccttga gttgatccgg agtctcttca tattcgaagg cagcctcgaa
      541 ctcgtgatac agagtcgagg cgtgtccgta ggactgccgc ttgacgagtt cgcgattcgc
      601 atagagatcg atcaattcat gcgccatctc ctcgatgtct tttttgaccc gcgcagttgt
      661 tttcgcccaa ctggtaccgc ccagtcggtc gagccgcggg acatgccctt ccgcgccgct
      721 gtaccgctgc acctgattga gccgatcgag agggacatac agtttgtcgc taccggcaaa
      781 ctcgaggatg agatagtcgc tgtcgaaatc ctggacggac aggcgtttga gcccttcata
      841 tttggcgatg ccatgctgca catgaaccac gtagtcgccg acgcggagat cttccagtga
      901 cgacaagaac gtggcagcct tactttttgg ctgaggttta tggcgggcgc ctttggcaaa
      961 gagttcctcc tcggtcaaca cagccaggcg gagttctgtc gagacgaacc ctgccgacag
     1021 atccccgtgc aagatgtaaa agggcgacct ggcggtggag gtcgcggtcc acgcagccgg
     1081 cgtccattcc atcgccggca cgtcgtgttc gtgcaagagt gcaagcagcc ggtctacttg
     1141 tccacggctt cttgcgacaa gcaccacccg gcattcggtg cgtatccgat cgaggatcac
     1201 cagggtttga ctgaagggcg tcccgcgcgt gctgagtccc gcggtggcag gcgtctgagc
     1261 agaaaacgtt tcgaccggtg accacgaggc atcggtctcc gcaagcggtt ccagagcgag
     1321 gatgggccag cctgagatcc ggtctgtgag ggcatcccac gactggaaca gtttttccgg
     1381 cgacgggtag gaaggcgatt ctttcagatc ggaatggtgc aagtatccat cgtcgatctt
     1441 gtcccacagg gctgcgcact ccgtcttcaa ggccgccggc tggtcgaaga ccagcagcgg
     1501 tcgttcggtg aagaagtcga acagcgtggc caatcgtgcg tataaatcag gcgtccgcca
     1561 ttcagcatcc ggaggaatag gcgagagcga atcaagatca ttttccgagc gcagatattc
     1621 tcgggctggg agcaccagcg cgcgatctaa ttttgccgtc gacttctgtg tggcgggatc
     1681 gaagagccga agggaatcga ttgtttcacc aaggaactcg actcgaagcg ggtcggcata
     1741 cgcggtggag aaaatgtcca cgatcccccc gcggatgctg aactcaccgg gaatttccac
     1801 gacggacacg cgacgatagc ctaaccggag cagtcctccc actaaggctt cccgctcgag
     1861 tgatgcgccg cgcgtgaatt gcaatatcgt tccagtaaag gtctcgaccg gaagaagtcg
     1921 gtggatgaga gcgtcgatcg acgtgacgag ccgaattcgg ggcatcgtcc tggcgcgata
     1981 gagggcgttc atgcgccgag caatcaaacc gacatgaggc tccgtggctt cgtagggcaa
     2041 ggtttcccat ttggggaaaa aggcgaggga gtcgacggaa agaccagaaa gtgtcgcgaa
     2101 gaaacagagg tcgtcgtgaa ggcgctcagc ggcttcgtcc gaacggctga cgatgagcca
     2161 aggtctggtt cccagcacgc tggtctgagg atgagtcagc gccgatagag caaaccccgc
     2221 tgtcgagcca tgcaaccccg tcaggcacgg tcggcctccc tcactcgcca gcgcggtggc
     2281 aatcggagcg aaccactggg gaacggcctg cccgcgggtg atggacacag cttacgtttg
     2341 ggttgaacgg atacgatgaa tgagggtcgt ggtagagacg ccggggacca gcggaatcgt
     2401 cttcaccacg cctccgcgtc cttcgacgat gtcacgcccg acgatagtct cgaccgacca
     2461 atccccccct tttaccaata cgtcgggctg cagggcgcga atcagcctgg acgggtccgg
     2521 ttcggcgaat agaacgacat gatcgacgca tcccaacgct gcgagaacct ccgcacgttg
     2581 agactccgga acgatcggcc ggtcgggaga cttattcagg ctcttcaccg aagcgtcgct
     2641 gttcactccg acgacgagca gatcccccag tgctttcgca gcctggagat aacgcgtgtg
     2701 gccgacatgc atgagatcga aacagccgtt ggtgaacacg atgcgcttcc cctgggttcg
     2761 cgcctgtgcc aacagcggca ccaactgctc ggtggtcacg accttggatg tcatgaagcc
     2821 atcataccta tcgagaagac ggatggaaag agcgtgaacg gttcaggaat cttcgcgtgt
     2881 cacgccgccg aggcccatca ccgatggccc atattgcgcg atgatggtat ccatcgcttc
     2941 gacagctttc gcgcgctgcc gatcgaagag cccgggcgcc gtagggctca gcgatgagag
     3001 ggctaagcct gcaaggcggt actttgtttc cggtacgagc agctcatgta aggtccgttg
     3061 gacgacgggc cacattgcag gatcgtagtt caaggggccg ggaaagcgcc gctgtttcgt
     3121 cgtgatcgta aaacgagcat ccttcagctt caccgtacag gatccggcgg ccaatccttc
     3181 ttggcgaaga ttgtgcgcga gtgcccgcaa gaacccccgt agggtctgtt cgatgaaggc
     3241 gcgatcatgc gtgtcatgtt cgaaggtggt ctcgtggctg agactcttcg attcgcgatc
     3301 ggcgcagact ggttcgcgat caatcccgcc ggcgagttcc cgtatttcca agaggtggtt
     3361 tccgaaaagt cggcgaagga ctccgtccca acgcatatcc aggagatccc cgatcgtccg
     3421 caagccgagc ggttccaatc gcgctgcggt cttcgggccg attcccggca gggctcggat
     3481 cggcagagga gcaagaaagg cctgttcagt tcccggttcg atcgcaacaa gcccatccgg
     3541 cttatgggag tccactgcga tcttggcaac agttttcccg gaggccagcg caacggtgca
     3601 tcgcagtccg gtcgcgtcga atattgattc tttcaccagt cggccgagcg cgagcgggtc
     3661 tggatggcgg gcctgtagat cggtggtctc cgcatagaat tcatcgatgc tgctccactc
     3721 ggtcaaggga aagaggcgat ccgtgacggc tcgcatctgg tcatgtaagc ggcgatacaa
     3781 cggacggtct ggggggatca gaaccaatcc cgggcagagc cggaatgcct cgatcgtcgg
     3841 catggcggaa cgcaccccat aaggccgcac cgcataactg gcagcggcaa tgatcccgcg
     3901 tggagtctgt cctccgacgg cgaccggctt gccggcgagg ctgggatcgg cgatgactgc
     3961 cgcagcagcg aacatcgcat caacatctcc gaagagaatc cgtcgggacc aatgcatggc
     4021 ccactcccag atacgtggag cttaaaaggt aaaggacaat tggatggact gcaagtcggt
     4081 ggcagagagc gcacgatggt cgccgggtgg ctcggaccgg tttggtgagg cttggcggcg
     4141 cgtcacggcc ctcagttctt ggagcgagcc gcggcacacg ccgcaatggt gccggcgagg
     4201 ttcgatggtc cgtcgctggc gacgatacac acggccgcat tgtcggcagc gccatgcaaa
     4261 gcgggtcagt gcgacgacct catcctgcaa cgaatggtac gtcgtaatgg ccagcatgcc
     4321 atctcgattc atctcggtca tcttccgcat gaagtcgggc ccgtggttgg gacgtcgctt
     4381 gaggatgtcg tactgccact gatgaatcat ttcgtgggcc aacgtgctcg tgatttcgtg
     4441 ctcgccatac gaagtcttcg caaaggcttg cgtcagcagc ggaaccgaaa gccgaatctc
     4501 ccgatgacgc ggcgcgctca tgccccagcg ttgtacggac atgcgtgggc cgatggtcgt
     4561 gacgaataag ccgaccgacg cggtcagacg ggtgctccac acaatatcga tgggagacag
     4621 aatccccttg aagtatcggg tgttgagatc ggcccagatg gcggcgagct tttccgtgga
     4681 acagggcagg ttgggagatg gtgccataag gaaattatcc caactcttcc tggagcgccg
     4741 ccgccaacaa cggcaccata atctcgtggt gaccgatcag ggagtaaccc tttccccctt
     4801 tttgggtggg gcgccgcacc acattcgtga gcggacgata gtgggacaaa aaatccatgt
     4861 tcacggtggt gatctttgtg agggcatggc ccaggttgcg gccgagcgac acggtcttga
     4921 gaaagacttc cggaaggatc accgcagagc caagattgag atagacgccg ccttccatgc
     4981 cggccaccac ggctgcgagt cggcggaagt cgagcagcga cgtcgcgccg atcgcggccc
     5041 catcagccga cggatgcata tgaatgatgt cggtcccgac ggccacatgc acggtgacgg
     5101 gaataccgag gcgcgcgccg gtggcgagga tactcgtctc gcgatgagga aattgtccct
     5161 ggtgccgatt gatatggtgc ccgacggctt cgccaagacc ttcacggtcg cgcgcgccgc
     5221 gcgcaatggc ctcgttgagc acgcgtccgg tttcctccgc catgccaaat cgtccttcgt
     5281 cgatctccgc atcaacctcc tcggaagtat ggcccatcag cgccaattca aaatcgtgaa
     5341 taatcccggc tccgttcatc gccaccgcag atacaatgct ctgattcatc cagtcgacga
     5401 tgatcgggtt gagccctacc ttgacgacat gcgcgccgat accgacgatc accggccgac
     5461 cttgccggtg cgcagcagcc atggtcttgg cgacagctcg aagggtcttc acagcgagaa
     5521 tatccggcaa ggcgcgatag aactggctga agcttcctcc ttgcttccac ggtttcgcga
     5581 aatccgaaac gcgcacctta ctgtgtcgtt tcttcagcgg ataagttttt aaatccgaaa
     5641 cgttgatcgg cgcaatgagc ggacgagttg gaaggtgggg aggggtccgt ctaggacgtt
     5701 ttgccaagga gctgttcctc tatgagttcg cacaagacat gaccgagcgt gatgtgactt
     5761 tcttgaatgc gggccgtgac tttcgatggg acgacgaacg gatactcgac catcccggca
     5821 agtttccctc cgctgccgcc ggtccaggca atggttgtca gtccaatcgc ccgagcggtc
     5881 tcgacacctt tgagcacgtt ggaggaattt ccgctggtgc tgattgcaat cgcgatgtcg
     5941 cctttctgtc catgggcgcg aatctggcgg gcgaatagct cgtcgtaacc ataatcgttc
     6001 gcgatacagg taatggcggc gacatcggtc gcaagggcga tcgccggcag ggggtctcga
     6061 tcacgatgat agcgcccgac aaactcggcg gcgatatggg ctgcgtcggt cgcgctgccc
     6121 ccgttgccga acaggagcac tttgcgacct tcacggaatg ccgtcgcgat cagtttgacc
     6181 acctgtacaa tgcggtcggc atggtcgcgc acaaactgtt gcttcaccga ggcgctctcc
     6241 tcgaacgccc tgatcgcctg ctccttcatc gaccgcgatt ctaggcgagg ggctctatcc
     6301 tgtcaaacag tcactcaagt ctgaagtatg ccggacgttg aattgaacag ggtccgggcc
     6361 gatgatatag tccgcgcgca atggcacaag accaaccagt caaagccctt gtcattacac
     6421 tgaccgatca tcccggtgcc gccgtctatt ctgttaatcg ccttcgtccc gactcgctct
     6481 gctttgtatt gcctgagtcg gcgaaatccc tcgtcgagtc atccgtgcag ccgaaaatcg
     6541 agcagatgcc tcgccggtgg gattgggtga tgctctcgga tgccgagcac tttccctctt
     6601 gctaccaaac ggtggctcgt tcattgctat cgatcctcaa agcttgggaa gtccagcctg
     6661 gtgaattggt cgtggatcta accggggcca cagccgcgat gagcggtagc ctggcgctgg
     6721 tcacaagacc cttcacgtcg cgcgcggtct cgctcgttcc agccgccgaa ggacgtgagg
     6781 gcgacgcagt ctccgtcgac ggtcacgcgt tcatctggct tcagatcaat ccctgggacg
     6841 aagccgcgac ggtatcccgc caggaagcct ttgattactt caaccgtggc gccttcgtgg
     6901 ccgccgctgc gctgtttcgg ctaatcgaaa tgagggtgag cggcggacaa aaaccgctct
     6961 accgcgcgtt cttcgatctc gccgaaggct atgatctctg ggaacggttt cactaccggc
     7021 aagcgtggga caaactgaag acatcgatca aggcgttaga gatggcctcc gtgtggggcg
     7081 gacctcccgg cgtgaaagag ctactgccct cgatgaaggc gaatgcggga ttcctcgaga
     7141 agctggtgct cgatccggcc gaagtgaaag atatcggcgt cctcgatctc ctcgccaacg
     7201 tccgacgacg cctcgatgtg gatcgcgatc ctgagcgggc catggttgcg ctcatgcgaa
     7261 ccctcgaagc cgtcgcccaa cgacaactgt tcaaacagta caaggtcaaa acgtgggatc
     7321 ttcaaccgga acagttgccg caagcgctcc gggaaacctg ccggacctgt tacctggacg
     7381 acgtggatgg gaagtacaag ttgccgttgc aggcacagtt tcgaacgctg gccggcttgg
     7441 gtgatcagct cgggcaaggg ttccttcgcg agtggccgac cctgaagccc ctgctcgacg
     7501 ccgccagcca tgccgttctc ggccacggct tcgaagcgat caaagccgaa cgggtccagc
     7561 agttgtacga ggttgtcctc aagctatcag gcgtcagcga gagctcgtta cccaggtttc
     7621 cggtcctgaa tttgtagaag ctctcaacgt tcaatcgtca gttctagggt gcgagttgta
     7681 gtccgacaac ttgagcgctt tatggaaatt cgcatctact atgaagacac cgattgcggc
     7741 gggatcgtct attacgccaa ttacctgaag tattttgaac gggcacggac ggaatacctc
     7801 gaagcgcgcg ggtactccgt ggcggctctg atggctgaag gcatcgtttt tgtcgtcgtg
     7861 cacgccgaag tggattattg ctctcctgcg cgatacggtg agacgctggt ggtcgaaacg
     7921 gtcgtttcgg acttgagccg agcttcgttg actttttcgc acgcggttcg tgagcgggcc
     7981 agcggtcgtg tcatcgtgga agggtcggct cggttggcgg tcaccgatgg cagcgggaaa
     8041 gtgaaacgcc tcgacaaggc gatcgttgtc gcgttagagt ccggttctgg gaggcgttcg
     8101 gatggataag ctcggaactt ggctcgctat cgccggcgtg gcaattggtt tcatcgtgct
     8161 tgcatggctg ttattttcag acaatccgtc cggcaacaac aagaagaagc cctaaacctc
     8221 aactccttcc aactgccttg gt
//
LOCUS       DATDNW010000081         3321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_149935, whole genome shotgun sequence.
ACCESSION   DATDNW010000081 DATDNW010000000
VERSION     DATDNW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149935"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VI359_02090"
     CDS             complement(<1..530)
                     /locus_tag="VI359_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEY6973073.1"
                     /translation="MPDLRRDPIVGRWVIISTDRSGRPHEFPQMQTARPISASLCPFC
                     PGQERLTPKEIMAYRSQPAEPNSPNWSIRVVPNKFPALQVEGDLGREGLGLYDRMNGI
                     GAHEVIIETPGHKDGLADMPAKRIEDVLWAYRDRILDLRKDLRFRYILIFKNHGAAAG
                     ATLEHSHSQLIALPIIP"
     gene            complement(649..2850)
                     /locus_tag="VI359_02095"
     CDS             complement(649..2850)
                     /locus_tag="VI359_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEY6973074.1"
                     /translation="MKTAHICFLWHMHQPYYTDPVTGFASMPWVRLHATKAYYDMAFL
                     LDKFPAVKATFNFTPSLLLQLQEIGAGTIRDLFQEYAQRPAADLTPEEQAFVIRHFFS
                     ANWATMVRPFPRYHELLVKRGLEVQGQDLERVAHQFSTQDLLDLQVWHNLAWFGYGTL
                     HRYPRLAALRTKNRNFTEEDKQEVLALQRVVIQEIVPMYRRLLDRGQIELTTSPFFHP
                     ILPLVIDTDTARRARPDLPLPARFSAPDDAEAQLRCAVDFHTATFGQAPAGLWPSEGS
                     VCPEMIPMLTRTGLRWLATDEGILARSLESAQQSWQRHRDLYQPYRVGPDGQAVTIIF
                     RDRDISDAFGFIYHKTTPESAAEDVLRRLRGILHDAPHEQALIAIILDGENPWEHYYD
                     GGERFLSNLYTALTQQTLNHDGLRASASAVSDALAAFPAAPPLTQLHSGSWINQDYKI
                     WIGHQEDNQGWELLGHTRARLTDQAPNLPADRAQAAWTELYAAEGSDWFWWYGDDFDT
                     AYKEEFDRLFRIHLRNVWMHMGLTPPDIFNRPICTVALKPDADRVMQPVALLAPTIDG
                     LVTDFFEWRGAGSINPHPPLGAMWKAEGIFTTILFGWSLDHLYLRLDPDDALQDRQSG
                     MVVEVSLLCPRQTFRLSFSLAPSGTDHFTLFHESSPDSWREIASYRSIARRTVLELGV
                     PIKDLGLEPGQEVRLSLMVLEHNLEVARYPYQNPTTLIVPGPEFDAASWRV"
     gene            2922..>3321
                     /locus_tag="VI359_02100"
     CDS             2922..>3321
                     /locus_tag="VI359_02100"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY6973075.1"
                     /translation="MDGVQQKFRNAVWEKALLYWAPVLIYAATIFYLSSLPKPQEQLP
                     EFLRDLSDKFLHLVEYGILGALWYRAFRWASGPRIAASAVLLAIVAGSIYGITDEVHQ
                     VFVPLRTASPLDWMADTLGSAIGACGLSWIE"
BASE COUNT          700 a          911 c         1027 g          683 t
ORIGIN      
        1 gggatgatcg gcagggcgat gagctgcgag tggctatgct cgagcgtcgc gccggccgcg
       61 gcgccatgat tcttgaagat caggatgtag cggaagcgaa gatcctttcg gaggtccagt
      121 atccgatccc gataggccca gagcacgtct tcgatcctct tggccggcat gtcggccaag
      181 ccatctttgt gaccaggggt ttcgatgatg acctcgtggg ccccgatccc gttcatgcgg
      241 tcgtacaggc caagtccttc gcggccgagg tcgccttcga cttggagggc aggaaacttg
      301 ttcggcacga cgcggatcga ccaattgggc gaattcggtt cggcaggttg ggagcgatag
      361 gccatgattt cttttggcgt cagccgttct tggcccgggc agaaaggaca gagtgaagca
      421 gagataggtc gcgcagtctg catctgaggg aactcgtgcg gacggccgct gcgatccgtt
      481 gaaataatca cccaccgacc gacaatggga tcgcgtcgaa gatcaggcat ggtgactccc
      541 agaccgccac acaatcgctg actactgagt cataagtggg tagtgtctca aacgaaaggg
      601 gatttcacaa ctcataagtc agtacctcgc actcagcacg gcttctcttc atacccgcca
      661 cgaggcggcg tcaaactccg gaccaggcac gatcagagtg gttggattct gatagggata
      721 tcgagccact tcaaggttgt gttccagcac catcaggctc aatcgaactt cctgtcctgg
      781 ttcgagcccg agatctttga tcggcacgcc gagttcgagg acggttcgtc gagcaatcga
      841 gcgataggag gcgatctccc gccaggagtc gggagacgat tcatggaaca aggtaaaatg
      901 atccgtcccc gagggtgcga gtgaaaagga gagccggaac gtttgtcgcg gacatagcaa
      961 cgagacctcc acgaccatgc cgctttggcg atcttgcaaa gcatcgtcag gatcgagtcg
     1021 aaggtacagg tggtccaggc tccagccgaa caggatggtg gtgaagatgc cctcggcttt
     1081 ccacatggct ccgagcggcg ggtgaggatt gatggacccc gctcctcgcc attcgaagaa
     1141 atcggtgacc agtccatcga tcgtcggtgc gagcaacgca accggttgca tcacacgatc
     1201 cgcatcgggc ttcagcgcaa cggtgcaaat gggtcggttg aagatgtccg gcggcgtcag
     1261 acccatgtgc atccaaacgt tacggagatg gatccggaag agacgatcaa actcctcttt
     1321 gtaggcggtg tcgaaatcgt cgccgtacca ccagaaccaa tcgcttcctt ccgccgcata
     1381 aagttcagtc catgcagcct gtgcccggtc agctggcaga ttcggcgcct gatccgtgag
     1441 gcgggctctc gtatggccga ggagttccca tccctggttg tcttcctgat gtccaatcca
     1501 aattttgtag tcctggttga tccaggaccc ggaatgcagc tgagtcaatg gtggcgcggc
     1561 cgggaatgcc gcgagggcat ccgagacagc cgacgcggag gcccgcagcc cgtcatgatt
     1621 caaagtctgc tgggtcaacg ccgtgtatag attcgacagg aaccgttctc ctccgtcgta
     1681 atagtgctcc caggggtttt caccgtcgag aatgatggcg atgagggcct gttcgtgtgg
     1741 ggcatcatga aggatgcctc gcagccgtcg cagcacgtcc tccgccgccg actccggtgt
     1801 cgttttgtga tagatgaatc caaacgcatc agagatgtca cggtcgcgaa agatgattgt
     1861 gacggcttgt ccgtcggggc cgacacgata gggttgatag aggtctcgat gacgttgcca
     1921 ggattgctgc gccgattcca acgagcgagc aaggattcct tcatcggtcg ccaaccagcg
     1981 caaaccggtg cgagtcagca tcggaatcat ttccgggcag acggaccctt ctgacggcca
     2041 caaaccggcc ggtgcctgtc caaacgtcgc ggtgtgaaag tccacggcgc agcgcagttg
     2101 cgcttcggca tcgtctggcg ctgagaagcg ggccgggagt ggcagatcag gcctcgcccg
     2161 acgcgccgtg tcggtatcga tgaccaaggg aagaatcggg tgaaagaacg gactggtggt
     2221 gagttcgatc tgcccgcgat cgaggagccg ccggtacatc gggacgatct cctggatgac
     2281 gacacgctgc agggccaata cctcttgctt gtcttcctcg gtgaagttgc gattcttggt
     2341 tcggagggcc gccaggcgag gatagcgatg aagcgttccg tatccgaacc aggccaggtt
     2401 gtgccagact tgcagatcta aaaggtcctg cgtcgaaaac tgatgagcga ctcgttccag
     2461 atcttggccc tgcacttcca atccgcgttt aaccaagagt tcgtggtagc gggggaaagg
     2521 ccgcaccatc gtcgcccaat tggcggagaa gaaatggcgg atgacgaaag cctgctcttc
     2581 gggcgtcaga tccgcggccg gccgctgggc atattcctga aacaggtcgc gaatggtgcc
     2641 ggccccgatt tcctgcaact gcagcagcag ggacggagtg aaattgaacg tggccttgac
     2701 cgccggaaac ttgtccagca gaaaggccat gtcgtagtag gctttggtgg catggaggcg
     2761 aacccagggc atactcgcaa accccgtcac ggggtccgtg tagtacggct gatgcatatg
     2821 ccaaagaaaa cagatgtgtg ctgttttcat agacgaagtt tattgcggac ttctacgcaa
     2881 gtatgtcata gggttggagg ctgcgcaatc aacacaacaa tatggatggg gtccaacaga
     2941 aattcaggaa cgcggtctgg gagaaggcgc tcctctactg ggcgcccgta cttatctacg
     3001 cggcgacgat tttttatctc tcctcgctcc cgaaaccgca ggaacagctc ccggagtttc
     3061 tacgcgactt gagcgacaag ttccttcacc tcgtagagta cggcatcctc ggcgccctct
     3121 ggtatcgcgc cttccgctgg gcttccggtc cgaggatcgc ggcatcggcg gttcttttgg
     3181 cgattgtggc agggtccatc tacggcataa cggacgaagt gcatcaggta tttgtgcctt
     3241 tgcgcacggc gagcccgctc gattggatgg cggacaccct cggctctgcc attggcgcgt
     3301 gcgggctgag ttggatcgag c
//
LOCUS       DATDNW010000082         4583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_488377, whole genome shotgun sequence.
ACCESSION   DATDNW010000082 DATDNW010000000
VERSION     DATDNW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488377"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="kdsA"
                     /locus_tag="VI359_02105"
     CDS             complement(<1..770)
                     /gene="kdsA"
                     /locus_tag="VI359_02105"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY6973076.1"
                     /translation="MTHEVDLGTFKAGAGHRPFLIAGPCVIESEQLVVETAGRIAEIA
                     KTLGMPYVFKSSFDKANRTSIKSFRGPGIEKGLAVLKKVKDQLGLPVLTDVHTEEQAA
                     EAGKVVDVLQIPAFLCRQTDLLIAAAKTGKVVNVKKGQFLSPQEMGNAVKKVEECGNR
                     RIILTERGSSFGYNNLVVDMRSFPILRSFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGVDGFFMEVHPNPDDALSDGPNMVPLHQ"
     gene            complement(787..2388)
                     /locus_tag="VI359_02110"
     CDS             complement(787..2388)
                     /locus_tag="VI359_02110"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY6973077.1"
                     /translation="MSKFIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERYTSLTLTKENNYTTGRIYHSVITKE
                     RRGDYLGGTVQVVPHVTDEIKQCIVRISKGMDVTIVEIGGTVGDIESLPFLEAIRQMP
                     YDVGRDNVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYIPPE
                     LKAKIAMFCNVEKDAVITAKDVDTIYEVPIVFRKEGLDELIVRQLRLETGPPNLREWD
                     AMVQKIKHPKHEVSIALVGKYAGLRECYKSLAEALVHGGIDHETRVNINWIESEDVER
                     QGTERILRDADGILIPGGFGARGIEGKITTIKYAREHQVPFLGLCLGMQCATIEFARN
                     VAGLAGANSAEFDEHSPHPVIHLMPDQHSVSDKGGTMRLGSYLCKLGEGTLAQKMYGV
                     DEVRERHRHRYEFNNAYRDVLTAKGLVLSGLSPDGRLVEIVEMRNHPWFLATQFHPEY
                     NSRPHHPHPLFSGFVGAALRRKCGH"
     gene            complement(2438..3238)
                     /gene="kdsB"
                     /locus_tag="VI359_02115"
     CDS             complement(2438..3238)
                     /gene="kdsB"
                     /locus_tag="VI359_02115"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY6973078.1"
                     /translation="MTNGRSSVSVVIPARYGSSRFPGKPLVKLGAKPLIQHVYERAAA
                     CAVVSDVVVATDDERIKQSVEEFGGRVILMTGEYRTGTDRVAAVASMLPGEYFVDLQG
                     DEIPVDPNLLTDLIEPFVASGAEIGTLKRAIESTGELQNPAVVKVVTDQQGRALYFSR
                     SPIPMVREDPSRCSTIGLGYIHLGVYSYRRNALLKFAALPTGVLEDAEKLEQLRALEH
                     GMVIRVWETTRASLRVDTPEDAVKVSEQLRLAEMAAHGMTLNRAVPSR"
     gene            complement(3231..4211)
                     /gene="rfaE1"
                     /locus_tag="VI359_02120"
     CDS             complement(3231..4211)
                     /gene="rfaE1"
                     /locus_tag="VI359_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY6973079.1"
                     /translation="MSAKALRQYIQRFPQASVLVIGDLILDHYIWGRVSRISPEAPVP
                     VVHVDSESLKLGGAANVFNNILALGGKADLCGVIGSDETGRLLLKELGSKRSGRGGIV
                     IDHERPTTRKSRVIAHNQQVVRYDVEQRDELKKSLQQRILRYVESRVRELSCLVVSDY
                     AKGVVTTTLMSELTRLASLRRIPVVVDPKVEHFGYYKGATVITPNHLEATQAAGVHGD
                     DDQAIDQAGAIIRQRLGCQSVLVTRGEKGMSLYEADGVSWHIPTQAQQVYDVTGAGDT
                     VIGTLALALSTGATMRDAAQLANQAAGIVVGMIGTATVSAQQLSEALGDD"
     gene            complement(4268..>4583)
                     /gene="lepB"
                     /locus_tag="VI359_02125"
     CDS             complement(4268..>4583)
                     /gene="lepB"
                     /locus_tag="VI359_02125"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY6973080.1"
                     /translation="VQIRNKVVLVNGRPLDDKGFTQRIDPGIIDGTINPRDNFGPVTV
                     PEGAYFVMGDNRDQSLDSRFWGFVREEKIRGKAFRIYWSWSGQGKWTEWVRWERFGKA
                     IQ"
BASE COUNT          977 a         1434 c         1126 g         1046 t
ORIGIN      
        1 aactgatgca gagggaccat gttcgggcca tcggagaggg cgtcatcggg gttcggatgc
       61 acctccatga agaagccatc aacgcctgct cccgctgccg cacaggcgag cggttcgacg
      121 aattctcgtt ggccgctgga cttcgtcccg ccgccgccgg ggagttgcac actgtgggtt
      181 gcatcgaaga cgaccggata gccaaaactt cgcaggatcg ggaacgaccg catatcaacc
      241 acaagattgt tatacccgaa cgaagaaccc cgttcagtga gaataatgcg ccgatttccg
      301 cactcttcca ccttcttcac cgcattgccc atttcttgag gcgagagaaa ctggcctttc
      361 ttcacgttga cgaccttgcc ggtcttggcc gcagcaataa gaagatccgt ctgccggcag
      421 agaaacgcgg gaatctgcag cacatccacg actttgccgg cctcagctgc ctgctcctct
      481 gtatgcacat ccgtcaaaac aggaagaccc agttgatcct tgaccttctt gagtaccgcc
      541 aggcccttct caatgccggg accacggaag gacttgattg acgtccggtt cgccttgtcg
      601 aacgacgatt tgaagacata gggcatgccg agcgttttgg cgatctcggc aatacgccca
      661 gccgtttcca ctacgagctg ctcgctttcg atgacacagg gaccggcgat cagaaacgga
      721 cggtgtcccg cgccggcttt aaatgttccg agatcgactt cgtgcgtcat gatttaggtc
      781 tcaaacctaa tgaccgcatt ttctccgcag cgctgcaccc acaaatccgc tgaagagcgg
      841 atgaggatgg tgcggacgcg agttatattc cggatggaat tgcgtcgcca agaaccaggg
      901 atggttcctc atctcgacga tttcaactaa tcgtccatcc ggtgagagcc cgctcagtac
      961 caatcctttc gccgtcaaga catcacggta ggcattgttg aattcatacc gatgtcgatg
     1021 tcgttcgcgg acttcgtcca cgccatacat cttctgcgcc aacgtgcctt cgcccagctt
     1081 gcagagatac gaccccagcc gcatcgtccc acccttgtcg ctcacgctat gctgatccgg
     1141 catcaaatga atcacaggat ggggagagtg ctcatcgaat tcggcgctgt tcgccccggc
     1201 aagcccggcc acgttgcggg caaattcgat cgttgcgcac tgcatgccca aacagagccc
     1261 gaggaacggg acttgatgtt ctcttgcgta tttaatcgtc gtgattttcc cctcaattcc
     1321 acgcgctccg aaacctccgg gaatcagaat gccgtcggcg tcccgaagaa tccgttcggt
     1381 tccctggcgt tcgacatcct ctgattcgat ccagttaatg ttaactcgcg tttcgtggtc
     1441 aatgccgccg tgcactaacg cttcggcaag acttttgtaa cactccctca gtcccgcata
     1501 cttccccacc agcgcgatgg atacttcgtg tttgggatgc ttgatcttct gaaccatcgc
     1561 gtcccattca cgcagattgg gcggaccggt ctccagacga agctgtcgta cgatcaattc
     1621 atccaggcct tctttccgaa atacaatcgg gacttcgtaa atcgtgtcca catccttggc
     1681 cgtaatgacc gcgtctttct ccacattaca aaacatcgcg atcttggcct tcagctccgg
     1741 gggaatatac cggtccgtcc ggcataagag gatgttcggc tgaataccga tctcgcgcag
     1801 cttattcacc gaatgctggg ttggcttcgt ttttaattcg cctgccgccc cgatgtaggg
     1861 caccagcgtg agatgcacgt acagaacatt gtcgcgaccc acgtcatacg gcatctggcg
     1921 aatagcttcg aggaacggca gactctcgat gtcgccgacg gtaccgccga tttccacgat
     1981 cgtgacatcc attcccttgg agattcgtac gatgcattgc ttgatctcgt ccgtgacgtg
     2041 cggcacgacc tggaccgtgc cgcccaaata atcaccacgc cgttccttgg tgataacgga
     2101 gtgatagatc cgccccgttg tgtagttgtt ttctttcgtc agcgtcaacg aggtgtatcg
     2161 ctcatagtgg ccgaggtcga gatccgtctc cgctccatcg tccgtcacat acacttcgcc
     2221 atgttgatag ggattcatcg tgccgggatc gacgttgatg tagggatcaa gcttcaagaa
     2281 cgtgattttg agaccccgac tttccaacag gttcccgatc gaggccgacg cgagtccttt
     2341 acccaaggat gagacgaccc cgcccgtcac gaaaatgaac ttgctcatcg tgcgctccat
     2401 ccgttttgac cgaatgattc gaacccgcat accatagtca gcgggagggc acagcccgat
     2461 tgagggtcat gccatgcgcc gccatctcag ccagtcgaag ttgctccgat accttgaccg
     2521 catcttccgg tgtatccaca cggagcgagg ctcgcgttgt ttcccacact cgaatgacca
     2581 tgccatgctc caaggcacga agttgctcca atttctccgc atcttccaaa acacccgtcg
     2641 gcagcgcggc aaacttcagg agtgcattcc gtctgtagct atacacgcct aaatgaatat
     2701 agcccaaacc gattgtcgag caccggctgg gatcctcgcg caccattgga atcggagacc
     2761 gcgaaaaata taacgcccta ccctgctggt cggtcacaac tttgacgacg gcgggattct
     2821 gtagctctcc tgtggattcg attgcccgtt tcagcgttcc tatctcggct ccgctcgcaa
     2881 caaatggctc aatgagatca gtgagcagat tcggatctac gggaatctcg tctccctgca
     2941 gatccacgaa atactcgccc ggaagcatgc tggccacagc agccacgcga tcggtccccg
     3001 ttcgatactc tccggtcatc aagatgaccc gtccgccgaa ctcttctacg gattgcttaa
     3061 tgcgctcgtc gtcggtcgcg accaccacat cggataccac cgcacaggca gcagctcgtt
     3121 catacacatg ctggattaag ggcttggcac ccagcttcac cagcggtttg cctgggaaac
     3181 gcgacgatcc gtagcgagct ggaatgacga cgctcaccga ggatcggccg ttagtcatcc
     3241 ccgagcgcct ccgacagttg ttgcgccgac accgtcgccg ttccgatcat ccccaccaca
     3301 atccccgccg cctggttcgc cagttgtgcc gcatccctca tcgttgcccc ggtcgacaat
     3361 gccagcgcca atgtcccgat tactgtatcg ccagcgcccg tcacatcata aacctgttga
     3421 gcttgtgtgg gaatatgcca agaaaccccg tcggcctcat agaggctcat tcccttttcg
     3481 ccacgagtca ccaagacaga ctgacatccc agtcgttgtc gtatgatcgc cccagcttga
     3541 tcaatcgcct ggtcgtcatc cccatgcaca ccggcagcct gtgtggcctc cagatggttc
     3601 ggtgtaatca ccgtcgcacc cttgtaatag ccaaagtgtt ccactttcgg atcgacaacg
     3661 acgggaatcc gacgaagcga tgccaatcgt gtcagctccg acatcaacgt tgtcgtcacc
     3721 actcccttcg catagtcgga gacgacgaga cacgacagtt cccgcacacg tgattccacg
     3781 tagcgcagga ttcgctgttg cagagacttc ttgagctcgt ccctctgttc cacatcatag
     3841 cggacgactt gttgattgtg cgcaatcact cgcgattttc tggtcgtcgg acgctcatga
     3901 tcgataacga tcccgccacg gcctgatcgt ttagatccca gttccttaag caacagccgg
     3961 cctgtttcgt ccgagccaat cacgccgcag agatccgcct ttcctcccag agccagaata
     4021 ttattgaaga cgttcgctgc accgccgagc ttcaacgatt cagaatccac gtgtacgacc
     4081 ggaaccggag cttccgggga aatccggctg acgcgtcccc atatgtaatg gtcgagaatc
     4141 aagtcaccaa tgaccaatac tgaagcctgt gggaatcgct gaatatactg ccggagcgct
     4201 tttgcagaca ccctacctcc ctgattacct gcgctctggc gagatatcac ttggcgactg
     4261 cccatgctta ttggatggcc ttgccgaatc gttcccatcg cacccactca gtccacttcc
     4321 cctgcccgct ccaggaccag tagatcctga aggccttgcc acgaatcttt tcttcccgca
     4381 caaatcccca gaatcgactg tccaaacttt ggtcgcggtt atcgcccatt acaaaatagg
     4441 ctccttcggg cacggtcacc ggcccaaaat tatcccgtgg gttgatcgtc ccgtcgatga
     4501 ttcccgggtc gatccgttgc gtaaaaccct tgtcgtccaa cggccgccca ttcactagca
     4561 cgactttgtt gcggatctgc acc
//
LOCUS       DATDNW010000083         3523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_897637, whole genome shotgun sequence.
ACCESSION   DATDNW010000083 DATDNW010000000
VERSION     DATDNW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897637"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VI359_02130"
     CDS             complement(<1..873)
                     /locus_tag="VI359_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY6973081.1"
                     /translation="MKKRSGPHSSPPPSGPAVAPAKTRTERDTMGELAVPAEAYYGVQ
                     TARAIENFPISPLRFPRAMIRAMGLIKRAAATVNESLHLLDKKQSDAIRRAATEVVEG
                     ALDAEFPVDIFQTGSGTSTNMNTNEVVSNRATELLGGTRGSKLVHPNDHVNLGQSSND
                     VIPTAIHIAAAETMQSQLLPALTRLHKALDRKAKEFDRIVKIGRTHLQDATPVRLGQE
                     FGGYARQIELGIQRVKRAQESLGEVALGGTAVGTGLNCHPDFAGKVLTIISKETGCSF
                     REASNHFEAQSTQDS"
     gene            1018..1251
                     /locus_tag="VI359_02135"
     CDS             1018..1251
                     /locus_tag="VI359_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973082.1"
                     /translation="MAKVEFHVEAYAGYKGEETPRSFTIDGISYTVAEIVDRWYSEAH
                     SYFKVRTTCGRRFVLRFDLDHNVWELVMREDAG"
     gene            complement(1308..1757)
                     /locus_tag="VI359_02140"
     CDS             complement(1308..1757)
                     /locus_tag="VI359_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6973083.1"
                     /translation="MTALMRWDPFRELEDMSDRLNRMFARPAARTNGKETMIVADWVP
                     TVDISETDGEYQIKAEIPDVKKEDVKVTLEDGVLTIQGERKQEKEEKGKKYHRVERSY
                     GSFVRSFSLPDVIDEEKVKAEFKDGVLNLHLPKSEKAKPKAIEVKVG"
     gene            complement(1897..3030)
                     /locus_tag="VI359_02145"
     CDS             complement(1897..3030)
                     /locus_tag="VI359_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY6973084.1"
                     /translation="MPHDFMPGLAGVPAAKSAVSDVDGQRGVLEYRGIRIEDLCAKSS
                     YLETAFLLLFGHLPTQEEMSRWTTDIARHRRIKFKIVDLMKCLPEQGHPMDALQAAVA
                     ALGMFYPGRNVKNAENNYWSVVRLVAKLPTIVAAWARLRHGDDPIPPRDELGFSENFL
                     YMLTEQTPPPLWNEILDDCFILHAEHTMNASTFTGLVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVQMLKEIGPPECARQYVEEKLRTKHKLMGFGHRVYKVKDPRATVLQGL
                     CERLFKESTPSPLYAVALKIEEAARELLQGKGIYPNVDFYSGIIYDKMGIGTDLFTPL
                     FAMSRVSGWLAHWLEQLNENKLFRPEQIYSGEHNQPYVPLDKR"
BASE COUNT          755 a          942 c          954 g          872 t
ORIGIN      
        1 tgaatcctga gtcgactgcg cttcaaaatg attggacgcc tccctaaacg aacaaccggt
       61 ttctttcgag atgatcgtca gtaccttgcc tgcaaagtcc gggtgacaat tgaggccggt
      121 gccgacggcc gtaccgccga gcgcgacctc gccgagcgat tcttgtgctc gcttcacgcg
      181 ctggatgccg agctcgattt gacgcgcata gccaccgaat tcctgtccga gtcgcacagg
      241 cgtcgcgtct tgcaaatggg tgcgaccaat cttcacgatc ctgtcaaatt ccttcgcctt
      301 tcggtcgagg gctttgtgca agcgggtgag tgctgggagg agttggctct gcatcgtttc
      361 tgctgcggcg atgtgaatgg ccgtgggaat gacgtcgttg ctggattggc cgagattcac
      421 gtgatcgttt ggatgcacca gcttgctgcc acgcgtcccg ccaagcaatt cggtcgcgcg
      481 gttcgagacg acctcattgg tattcatatt ggtcgaggta cctgatccgg tttggaaaat
      541 atcgacggga aactctgcgt caagcgcgcc ttccacaact tcagtcgcgg ctcgtctgat
      601 cgcatccgat tgtttcttgt ccaggaggtg aagggattca ttgaccgtcg ccgcggctcg
      661 tttaatcagt cccatcgctc gaatcatggc acgagggaat cggagcgggc tgatagggaa
      721 gttttcgatc gccctggccg tctgcacacc gtaatacgcc tcagcgggca cggccaattc
      781 acccatggta tcccgctcag tgcgagtctt tgccggagcc acggcaggtc cggatggtgg
      841 gggcgatgag tggggccctg accgcttctt catgattatg tctcctcgct gaaccgactg
      901 cgctaaaagg gaagggcatc ttaaacggtt gctgcccaga agctcaaggg ttgggaccca
      961 agagcattga ctgagttgtc ttgagtccgt taggaatcgt cgactggggt tgatggtatg
     1021 gcaaaggtcg aattccatgt cgaggcttat gcgggctata agggggagga aacccctcga
     1081 tccttcacca tcgatgggat cagttacacc gtcgccgaaa tcgttgaccg atggtacagc
     1141 gaagctcact catatttcaa agtgagaacg acttgcggac ggcgctttgt gcttcgtttt
     1201 gatttggacc acaacgtgtg ggaactggtc atgcgggagg atgcaggctg acaaaaagga
     1261 aaggcccgca aatgcgggcc tttcttcgga gatctcttca agaaaactta gccgactttc
     1321 acctcaatcg ccttcggctt ggccttctcc gatttgggca gatgaagatt caggactcca
     1381 tccttaaact ctgccttgac cttctcctca tcgatcacat caggcaggct gaagcttcgg
     1441 acgaagctgc cgtaggaccg ctcgacccga tgatacttct tgcccttctc ttccttttcc
     1501 tgtttgcgtt ctccctgaat ggtgaggacg ccgtcttcca gcgtgacctt cacgtcctct
     1561 tttttcacat caggtatttc cgctttgatc tgatactcac cgtcggtttc actgatatcg
     1621 acagtaggaa cccaatccgc cacgatcatc gtttctttcc cgttcgttcg tgcggcaggg
     1681 cgcgcaaaca ttcgattcag ccgatcggac atatcctcca attcacgaaa tggatcccat
     1741 cgcataagag ccgtcatggc aaacctcctt atgatttatt acccgtccat gttgcctaac
     1801 tgttcgctta cagtggcgct gcgcctgttc agctatctgg tgttccagat aattgcggtt
     1861 aaacgagagt caaggggggg agaatcaaag aactggctac cgtttgtcga ggggcacata
     1921 cggctggttg tgttcgcccg aatagatctg ttccggcctg aagagtttgt tctcattgag
     1981 ctgttccaac cagtgcgcca gccaaccgga aacacgcgac atggcaaaca gcggggtaaa
     2041 gagatcggtg ccaatcccca ttttgtcata gatgatgcca gagtagaaat cgacgttggg
     2101 ataaatgcct ttcccttgca gcagttcccg tgctgcttcc tctattttca gggccaccgc
     2161 gtataagggc gacggggtag attccttaaa cagccgttcg cagaggcctt ggagcacagt
     2221 cgctcgagga tccttgacct tgtacacgcg gtgaccaaat cccatcagtt tgtgcttggt
     2281 ccgaagcttc tcttcaacat attgacgtgc gcattctggc gggccgattt ctttcagcat
     2341 ctggacgact tcttcattgg cacctccatg caagggacct ttgagggcgc caatggatga
     2401 cgcgacaacc gtgtaaggat cggctagggt cgatgccgtg acaaggccgg taaatgtcga
     2461 ggcattcatc gtatgctcgg catgcaaaat gaaacagtcg tccaagatct cgttccacag
     2521 cggtggcggc gtctgttcgg tcaacatgta caggaagttt tctgagaaac ccagttcgtc
     2581 gcggggaggg atcgggtcat cgccgtggcg gagcctggcc caggccgcca caatggtggg
     2641 gagtttggcc actaaccgca cgactgacca ataattattc tcagcattct ttacgttccg
     2701 tcccggatag aacatgccga gggcagcaac cgctgcctgt aaggcatcca tggggtggcc
     2761 ctgttccggc agacatttca tcagatcgac gatcttgaat ttaatccgtc gatgacgggc
     2821 gatgtccgta gtccatcgac tcatctcttc ctgtgtcggg agatgcccaa aaaggaggag
     2881 aaacgcggtt tctagatagg aggactttgc gcaaaggtct tcaatacgga taccgcggta
     2941 ttccagcaca ccgcgttgtc cgtcaacatc gctgactgcg gatttagcag ctggcactcc
     3001 ggcgagtccg ggcatgaaat catgtggcat agttgtagtc cttgggtgac ccgtattagc
     3061 ttatcatgtt tgaaacagga tcatcttgaa atgagagatt tcgattggca tactgatttc
     3121 caattaaagt aatggacagg ataatgttca tccggatcat gatcgtagtt ggattagcta
     3181 ccctggctct cgccttaatt atgattgcac gcggttctca cacgggcacc ccgccgcctt
     3241 ccacgaagcc attgggcaca agcactctca gtcacggagg gccatctatc actccaccct
     3301 taaacgtatt gcccgctcga cccgctgata gcgcgaatga tcaggaccgt gcaggcgata
     3361 caaagtagac catgatatat ggccgggtct tcatcttcag tttcatgtca gttcttctgg
     3421 tcgccagccc atcggtacga gctgaagatg agcagctcat tttcgccgtg gtcagcgaag
     3481 tgcccaaaga ccggtctcgg gttgccgcaa ggatatctct caa
//
LOCUS       DATDNW010000084         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_897779, whole genome shotgun sequence.
ACCESSION   DATDNW010000084 DATDNW010000000
VERSION     DATDNW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897779"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VI359_02150"
     CDS             complement(<1..479)
                     /locus_tag="VI359_02150"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEY6973085.1"
                     /translation="MKKNGAAPRQASIQRATKETDIRVEWTLDGIGQGKISTSIRFFD
                     HMLELLAKHGFFDLTVQAKGDIDIDEHHTVEDVGIVMGQALNQALGEKAGIKRFGFAS
                     APLDETLAQVTVDLSGRPYLVYNVKLTDRKIKAFDLGLFEDFFQAFVTHGGLNLHVNL
                     "
     gene            complement(476..1759)
                     /gene="hisD"
                     /locus_tag="VI359_02155"
     CDS             complement(476..1759)
                     /gene="hisD"
                     /locus_tag="VI359_02155"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY6973086.1"
                     /translation="MKIVSSSDRTYATTVKKVAGRARVQGAAVEKSVKTILQAVERGG
                     DRAVFRYTKQFDRVTLKPATVRVTAEEIKEAYYRIRKDEGDALRFSAQRIAAFHERQR
                     TKTWMYQDHAATLGQLVTPIDAVGVYVPGGKAVYPSSVLMCAIPARVAGVPRIVMCTP
                     PQKDGINPYLLVAADLAGVTEIYRVGGVQAVGALAYGTATIAKVDKIIGPGNIYVATA
                     KRMLYGTVGIDMMAGPSELLIVADDNSKPAHIAADLLCEAEHDEDAQVYLVTTSHQLA
                     KDVAQLIGDQLKGLQREKIAAKSISRHAVAFVVTTMDEAIDLANEIAAEHVTLAVDEP
                     FDYLEKIRHGGAFFLGRYTPPSFADYVAGPNHVLPTGGTARFFSALSVHDYTKVSNIV
                     HCTKEELSKVKDHLIRLAHIEGFDAHAKSAQSRFS"
     gene            complement(1756..2445)
                     /gene="hisG"
                     /locus_tag="VI359_02160"
     CDS             complement(1756..2445)
                     /gene="hisG"
                     /locus_tag="VI359_02160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY6973087.1"
                     /translation="MLTIALSKGKLLDPTLEMFHRAGYASPSVMRDSRKLVFPCLEIG
                     VTFLIVRPTDVPTYVEYGAADVGICGKDVLLEQDSDVYEPLDLGFGACKIAVAALRGQ
                     GSRNRLSSKIRVATKYPNITEQYFNQRGVPVELIKLYGSIELAPLVGLADRIVDLVET
                     GSTLKAHDLVEVELIARSTARLIVNRASLKLKHAAVTELIRKFRAVGSAKRGFAPRRP
                     GCTAKRVRKRA"
     gene            complement(2450..>2671)
                     /locus_tag="VI359_02165"
     CDS             complement(2450..>2671)
                     /locus_tag="VI359_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973088.1"
                     /translation="VTGRTRLAGAPVMASDLRASAGLILAGLAAEGVTEVLRVYHLDR
                     GYERIEEKLRGLGAKIERRKEGMAITPVA"
BASE COUNT          533 a          836 c          701 g          601 t
ORIGIN      
        1 aggttcacgt gcaaattcag tccgccgtgc gtgacgaagg cttggaagaa atcttcgaac
       61 agccccaggt cgaacgcttt gattttccga tccgtcagct tcacgttgta cacgagatag
      121 ggacgcccgc tgagatcgac cgtcacctgc gccagcgtct catcgagcgg cgctgaggca
      181 aacccgaatc gcttgatccc agctttctcg cccaaagcct ggttgagggc ttggcccatg
      241 acgatgccga catcttcgac cgtgtggtgc tcatcgatat cgatgtcgcc cttggcttga
      301 acagtcaggt cgaagaaccc gtgcttggcc agcagctcaa gcatgtgatc gaagaaacgg
      361 atcgacgtac taattttccc ctgcccgatg ccgtccaatg tccattcgac acggatgtct
      421 gtttccttgg tcgcacgctg aatcgaggcc tgcctggggg cggctccgtt cttcttcatg
      481 agaacctgct ttgggccgac ttggcatggg cgtcgaaccc ttcaatgtgt gcgagacgaa
      541 tgagatgatc tttcaccttg ctcagctcct ctttcgtaca atgcacaatg ttgctgacct
      601 tcgtatagtc atggacggat aaggcggaga agaatcgggc cgtgccacct gtcggcaaga
      661 cgtgattcgg gccggccacg taatccgcga acgacggggg ggtgtaccgc ccaaggaaga
      721 acgcccctcc gtgcctgatc ttttccagat aatcgaaggg ttcatccact gcaagcgtca
      781 cgtgctctgc cgcaatctcg ttggccagat cgatcgcctc atccatcgtt gtgacgacaa
      841 aggccaccgc gtgacgcgag atcgactttg ccgcaatttt ttcgcgctgt aatcctttca
      901 actgatcacc aatcagctgc gccacatcct tcgccaactg atgggacgtc gtgactaaat
      961 acacctgtgc atcttcatcg tgttccgcct cgcagagtag atcggccgca atatgcgctg
     1021 gcttggaatt gtcatccgca acgatgagca gttcgctcgg acctgccatc atatcgatcc
     1081 caacggtccc atagagcatc cgttttgccg tagccacata gatgttgccc ggtccgataa
     1141 tcttgtcgac tttggcgatt gtggcagtcc cataggccaa tgccccgacc gcctgaaccc
     1201 ctcccacgcg atagatctcc gttacgccgg cgagatccgc cgcgaccaac aggtagggat
     1261 tgattccgtc tttttgtggc ggcgtgcaca tgacgatgcg tggcacgccg gcgactctgg
     1321 cggggatggc acacattaac accgaggaag gatacaccgc cttaccaccc ggcacataga
     1381 ccccaaccgc atcgatcggc gtgaccagtt gccccaacgt ggccgcatgg tcttgataca
     1441 tccaggtttt cgtgcgctgg cgctcgtgaa acgccgcaat gcgctgcgcg gaaaagcgca
     1501 aggcgtctcc ttcgtccttg cgaatccgat agtaggcttc tttgatttct tcagcagtca
     1561 cccgaacggt ggccggcttc aacgtcacgc gatcaaactg tttggtgtac cggaacacgg
     1621 cccggtcgcc tcctcgctca accgcctgca gaatcgtctt cacggacttt tccaccgccg
     1681 caccctggac ccgtgcgcgt cccgcaactt tcttaaccgt tgtggcatac gtgcggtcgc
     1741 ttgacgatac gattttcatg ctcgtttgcg cactcgtttt gcggtgcagc ccggcctccg
     1801 tggagcgaag cccctcttgg ctgatcccac cgcccgaaat ttgcggatca attcggtcac
     1861 cgctgcatgt ttcagtttga ggctggcccg attcacgatc aacctggccg tggagcgggc
     1921 aatgagttcg acttcgacga gatcgtgtgc ttttaacgtg cttcccgttt cgaccaggtc
     1981 gacgatccgg tcggctaaac cgacgagtgg cgcgagttcg atcgatccgt aaagcttgat
     2041 gagttcgacc ggcacgccgc gctgattgaa atactgttcc gtgatgttcg ggtatttcgt
     2101 ggccacccga atcttcgacg acaatctatt gcgcgaccct tgcccccgca gcgcagcgac
     2161 ggcaatttta cacgctccga accctaaatc caatggctca tagacatcgc tatcctgctc
     2221 cagcagcaca tctttcccac agattccgac gtctgccgcc ccatattcga cataggtcgg
     2281 tacatcggtc ggtcgcacga tcagaaaggt aaccccgatc tccagacagg ggaagaccag
     2341 cttccggctg tctctcatca cgctcggaga ggcataccca gcccggtgga acatttccag
     2401 cgtcggatcg agaagcttac cttttgataa tgcgatcgtc agcataggct catgccaccg
     2461 gggttatggc catcccttcc ttcctgcgct caatcttggc ccccaatccg cggagctttt
     2521 cctcgatccg ctcgtagcca cgatccagat ggtaaacgcg caagacctcc gtcacgcctt
     2581 ctgccgccag accggcaaga atcaaccccg cgctggctcg aagatcggag gccatgaccg
     2641 gcgcgccggc gagacgtgtt cgcccggtca c
//
LOCUS       DATDNW010000085        11649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1033256, whole genome shotgun sequence.
ACCESSION   DATDNW010000085 DATDNW010000000
VERSION     DATDNW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033256"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1079
                     /locus_tag="VI359_02170"
     CDS             54..1079
                     /locus_tag="VI359_02170"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY6973089.1"
                     /translation="MLKKKSAYVVAIIGATGAVGKESLDILEERKFPLESLRLFASKR
                     SAGEVMACQGKEWKVEELTTTSSFAGIDIAFISATDAISREYGPHLAAAGIVVIDDSG
                     TFRMEADVPLVVPEVNASALQTIPRGIISIPNCTTTPLVMALKPIHEAAGVKRVVVTT
                     FQSVSGTGSAAMNELLDQTRALMSFRNVKAEVYPYQIAFNLLPHIGSFSEGGDCSEEV
                     KIVRETQKILDAPHLRVTSTTVRVPVMRCHSESINVELERPLNPNEARAALAAMPGVI
                     VYDDPGKKLYPMPLDATGKDEVFVGRVREDPTIANGLNLWVVSDNLRKGAALNAVQIA
                     ECLVRGR"
     gene            1076..1330
                     /locus_tag="VI359_02175"
     CDS             1076..1330
                     /locus_tag="VI359_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY6973090.1"
                     /translation="MTEWEGLGRILIAIGLAVATVGVLIVLNDRIPGIGTLFGWFGKL
                     PGDVSIKRDQFSFHFPIVTSLVLSVVLSLVFYLVSWLFRR"
     gene            1351..2541
                     /locus_tag="VI359_02180"
     CDS             1351..2541
                     /locus_tag="VI359_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY6973091.1"
                     /translation="MLTLVVGSLLMESLSGVAAAQSIRVLLSAEVQRLDVRSEGPIWM
                     TDSKDRGRAIKSAAQITANGSGFLLNGVRLASEQLTLRAGEQGLTLIYRKQTGNGHGV
                     TGSGSDPGTTVPVSGLIHLVRKGKGFLLINQVDLEEYVKGVVPAEVNSTWHPEMLKSQ
                     AVAARTYALYQQMLSATREYDVVATVQDQVYRGRHGIDAGVQQAVEATRGLVITYQNA
                     PIYAAFSSTAAGLTEDAMNVWSKDLPYLKGVECPFDLESPFYQWKASFKIDALEQNLR
                     QQGFPVGTIATLTPVTFSRAGRVATLRILHSGGELVLRGEDLRKAIGYSLVPSTQFTV
                     DSIGQDIILSGYGAGHAVGMCQWGAKQLAELGYSYSTILNYYYPGTELQNMALTKPPT
                     PPSS"
     gene            2541..3563
                     /gene="queA"
                     /locus_tag="VI359_02185"
     CDS             2541..3563
                     /gene="queA"
                     /locus_tag="VI359_02185"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY6973092.1"
                     /translation="MQLSEFDFPFDASLIATHPVLPRDEARLLVLYTNDQSLAHRSIK
                     ELPELLNPGDLLVVNDTKVRAARVTGWKHPSGGSIEMLFVRELSDHTWEVMVKGKLTP
                     GQSIEVSPDAHVIVLGREADRTTVRIETARAIPEFFRLYGRMPLPPYIKRLPTDEDRD
                     WYQTVFAKDEGAIAAPTAGLHFTPDLLGRLKSRGVGLTTVTLHVGPGTFKPVTVDRIE
                     DHRMGREWIDVGPEAVQAIERTKSKGGRVIAVGTTVVRALETAAQTQGQIRPYRGETS
                     LFITPGFPFKVVNALLTNFHLPRTTLLMLVSALAGRPLLLQAYEEAVRARYRFYSYGD
                     AMLVIE"
     gene            complement(3615..4313)
                     /locus_tag="VI359_02190"
     CDS             complement(3615..4313)
                     /locus_tag="VI359_02190"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY6973093.1"
                     /translation="MIKILRYTHDRFPWLLAAAMGFIAVTFIVGMGWWGFGESSGNVV
                     ASVGDLTVSRDEFRRAYENTYRVYKEKVPGEFKDETIKQYVVEQLVENRMWLIVAKEM
                     GLTVSNDELRDVITRIPDFQKNGTFDPEIYRRLLAANHLTPSVFEAMETKDILTTKAK
                     MVVRDAVSLTPSELAEAQALMLRQPESEPAKAAAAKDRAVQDMLFQKQQRALMAYAEA
                     LKTRLPITIHRELL"
     gene            4542..5000
                     /locus_tag="VI359_02195"
     CDS             4542..5000
                     /locus_tag="VI359_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY6973094.1"
                     /translation="MAEEVGRIHPAGSAVYPKAQVLNRCIAKLIDCFIVGAASEILTP
                     IGFLAGLAYVLVADGFAGGQSIGKRLIGLQTILPGTRETAGFRESIIRNLPFAAAQVA
                     FAVPYVGWIASVAILAFETVLVVGNEQGRRVGDEVAGTQVVDAGRLALPD"
     gene            5045..6094
                     /locus_tag="VI359_02200"
     CDS             5045..6094
                     /locus_tag="VI359_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY6973095.1"
                     /translation="MGFASDIFGWFSNDLAIDLGTATTLVYVHGKGIVLNEPSVVAVE
                     KKTEKVLAVGADAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKRFIQKAHNRSAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDVGGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVIDDAEVREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLAKVSVMSQANTFR"
     gene            6108..6956
                     /gene="mreC"
                     /locus_tag="VI359_02205"
     CDS             6108..6956
                     /gene="mreC"
                     /locus_tag="VI359_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY6973096.1"
                     /translation="MWMVNFRSPSGTRRLVLASIALLLVALFLLPSQSQGLLQYVSSP
                     LGEVLSLPLQVISSIDHGISELWSGYVALQGVREENEQLRKDMDLLRGQNSQLREAAA
                     ATERLKSLLQFKEQAPSAIVAAQVIGRDATNSYRSVIINKGESDGIRSDMGVVTPVGV
                     VGRVVKTTGATAVVLLVTDPNNAIAGLIQRTRDEGIVEGTHQGLARLKYIPLLSTVRN
                     GDRVVTSGLVGGFPRGLPIGTITGIDRAEGALFQSAELMPDVDMGRLEEVLVIQDPRG
                     QSSNGG"
     gene            6986..7480
                     /locus_tag="VI359_02210"
     CDS             6986..7480
                     /locus_tag="VI359_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973097.1"
                     /translation="MNILIYIGLFLLLIPLQTTLLPHVTVWEVKPDMGLVAACFVGLF
                     AGELRGVVVGLAIGWALNLFSAGELWISLVTKAGAGFCAGMMGRHLTYVSPTLMGIGV
                     FAVSCLSGLVELLTFTSLSESWWRVWSFMLPQACYDAVVAAGLYWIVSEWFVRERFSM
                     ASRY"
     gene            7510..9387
                     /gene="mrdA"
                     /locus_tag="VI359_02215"
     CDS             7510..9387
                     /gene="mrdA"
                     /locus_tag="VI359_02215"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6973098.1"
                     /translation="MMTSIGFHDSELGEVQRRLAVLRIGLLLVVALLAFRLWHLQIRE
                     GPYYRDLSENNRTRSVTLEPARGLIYDRQGVLLANNVPSFSLYVSLEDVKDREALINT
                     LTNLIGLDPAVVQKKLAGRGGKQLPRKIKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPGGVTAAHLLGYVGEVSAEQLEKPEYADLHQGSIVGQYGVEKFFDRHVRGLAGLKS
                     VEVDALGHEKRTVVVDQPQAGNDLYLTVDVRLQALAEKLLGEESGAIVALDPTNGDIL
                     AMASRPAFDPNVLSRELTPKQWVEIVQDEGRPLNNRASQGQYPPGSTFKILMAAAALE
                     SKTVSPSSTVQCTGGYQFGKRVYRDWKAGGHGSVNLHQALVHSCDVYFYTVGQRMGID
                     TIASYAHQFGLGEETGVELPSERVGIVPSTEWKQKAKHEPWLPGETISASIGQGYVTV
                     TPLQMASLIGTVANDGVMYRPRLVQGIMDRTTSQLQQLPSTPKRKLPVRSQALELIQD
                     ALASVVKEGTGTRAKSSIVTIAGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVE
                     SPKIAVAVLAEHMGHGGSAAAPLAKELIELYAKLAPQTPALTAKLEPVAAVSVDGVVT
                     R"
     gene            9396..10505
                     /gene="rodA"
                     /locus_tag="VI359_02220"
     CDS             9396..10505
                     /gene="rodA"
                     /locus_tag="VI359_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY6973099.1"
                     /translation="MMDTRGVDNFDIRFIALVFLILSIGVLSIFSVTHDQTGPGLPFF
                     AKQIIWILLGTVAFLIMLVWDYHRVARLAYPAYAIILVLLAVVLVEGKTSRGAQRWIP
                     IGPFAFQPSEFAKLILILVLAHYYSKVPRVGWLQRVVVPGLLMLPGLLLILKQPDLGS
                     GLSFLAVYAAMLLIVGMRSQALGVILLFSLMLFPFAWEVMWGSLHDYQRQRIMAFVDP
                     AYDPGGKGYHALQSRIAIGAGELFGKGLYGGTQSQLKFLPEGHTDFVFSVFAEEWGFL
                     GVLGLLILFVALIWLSLEIAAKAKDQLGALLAVGIICMLCFCVVVNIGMTAGMFPIVG
                     IPLPLMSYGGSATIMTMASLGLLLNVKRRRLSLFY"
     gene            10672..>11649
                     /locus_tag="VI359_02225"
     CDS             10672..>11649
                     /locus_tag="VI359_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY6973100.1"
                     /translation="MGVEIAISVAREETRVAVMDGGVVTDLFGDRAKQKDFVGNVYKG
                     KVAKVLPGMQAAFVDIGLDKAAFLHVSDLSLDSEPGDTLVDADEDDDKDSDMLRPRRQ
                     SSKPIEQLLSEGQELMVQISKGPIGTKGPRVTTYVSFPGRYLVFMPNVEHIGVSRRIA
                     RDEERARLKDIMKRVRHPGCGYIVRTVSEGVKEDELRSDVEFLHVLWQDILTKRAQLA
                     APALLHADLNLSFRVVRDLFGKKVERLWIDSRQEYEAVRDFVQRFSPEQTSRIHYYDK
                     EESLFDHLGVEQELTRALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVG"
BASE COUNT         2498 a         2979 c         3526 g         2646 t
ORIGIN      
        1 gacaccttta atcaccgcat cagagaaatc gaacagccct aggattgaga ggcatgttga
       61 aaaagaaatc agcgtatgtc gttgccatca tcggcgctac cggtgcggtc gggaaggaat
      121 ctctcgacat cctcgaagaa cggaagttcc cactcgagtc gttgcgcctc ttcgcctcaa
      181 aacgatcggc tggtgaagtg atggcctgtc aaggcaagga gtggaaagtc gaagagttga
      241 cgacgacttc ctcgtttgcc ggcatcgata tcgccttcat ttcggccacc gacgcgatca
      301 gccgagagta tggtccacat ttggccgcag cggggatcgt cgtcatcgac gatagcggca
      361 cctttcggat ggaggcagat gtgccgctgg tcgttcccga agtcaatgcg tccgcattgc
      421 agacaatccc gcgcgggatc atttcgatac cgaattgcac cacaacgccg ctcgtcatgg
      481 cattgaagcc gattcatgag gcggcaggag tcaaacgcgt cgtcgtgacg acgtttcagt
      541 ctgtgtcggg aaccggttcg gcggcgatga atgaactgct cgatcagacg agagctttga
      601 tgtcgtttcg caacgtgaaa gccgaggtct atccctatca aatcgcgttc aatctcctgc
      661 cccacattgg atcattttct gagggcggag attgttcgga agaagtgaag atcgtgcgcg
      721 agacgcaaaa gatattggat gcgccccact tgcgggtcac ctcgacgacc gtgcgtgttc
      781 ccgtgatgcg ctgccattcc gagtcgatca atgtcgagtt ggagcggccg ctcaatccaa
      841 acgaagcccg agcggcactg gcggcgatgc ccggtgtgat cgtgtatgac gatccgggga
      901 aaaagttgta tcctatgccg ttggacgcga caggaaaaga cgaagtattc gtggggcgag
      961 tccgggagga tcccacgatt gccaacggcc tgaacctctg ggtggtgtcg gataatctcc
     1021 gcaaaggagc agcgctcaac gcggtgcaaa tcgctgagtg cttggtgaga ggtcgatgac
     1081 ggaatgggaa ggtctcggca ggatcttgat cgccattggg ctggctgtag ccacggtcgg
     1141 agtgctcatc gtgctcaacg accggattcc agggatcggc acgttattcg ggtggttcgg
     1201 caagcttcca ggtgatgtgt ccattaaacg ggaccagttc tctttccact ttccaatagt
     1261 taccagcctc gtgctcagtg tggttctcag tctggtattc tatctagtat catggctatt
     1321 tcgccgctag atcgagtaaa gccaaggctg gtgctgaccc tagtggtcgg ttccctgttg
     1381 atggagtccc tctctggggt ggcggctgcc cagtccatcc gcgtcctgct gtccgctgaa
     1441 gtacagcgac tggatgtccg ctctgagggg ccgatctgga tgacggactc caaggatcgc
     1501 gggcgggcta tcaagtcggc tgcacagatc acggcgaacg ggagcggctt cctgctgaac
     1561 ggcgtgcgcc ttgcctccga gcagctgact ctcagggccg gagaacaagg actcacgctg
     1621 atctatcgca agcagacggg gaatggtcat ggggtgactg gatcaggaag cgatccggga
     1681 acaacggtgc cggtgagcgg actgattcat ctggtccgga aagggaaggg gtttctgctc
     1741 atcaaccagg tggatctgga agagtatgtg aaaggcgtcg tgccggctga agtcaattcg
     1801 acatggcacc cggagatgct caaatcgcag gcggtcgcgg cccggaccta tgccctctat
     1861 caacagatgt tgagcgcgac gcgggagtac gatgtcgtcg ccacggtgca ggatcaagtc
     1921 tatcgaggca gacacggcat cgatgccggc gtgcagcaag cggtggaggc gacgcgcggc
     1981 ttggtgatca cctatcaaaa tgctcccatc tatgcagcct tttcttccac tgctgccggg
     2041 ctgaccgaag atgccatgaa tgtgtggtcc aaggaccttc cctatctcaa gggtgtggag
     2101 tgcccgtttg atctggaatc gccgttctat cagtggaaag cgtcgttcaa gatcgatgcg
     2161 ctcgaacaga atcttcggca acaggggttc cctgtgggca cgatcgccac tctgacgccc
     2221 gtgacgttta gccgagctgg tcgggtggcc acgctccgaa tcctccactc cggaggagaa
     2281 ttagttctgc gtggggagga tttgcggaag gccattggat actcgttggt cccgagcacg
     2341 cagtttacgg tggactcgat cggacaagat attattctgt ccggctatgg agctggacat
     2401 gccgtgggta tgtgccaatg gggagccaag caactggccg aactaggcta ttcgtattcc
     2461 acgatcctca actattatta tcccggtacc gagctgcaga acatggcgct gacgaagcca
     2521 ccgaccccac cgtcctcctg atgcagttgt ccgagttcga ctttccgttc gatgcctcgt
     2581 taatcgccac tcacccggtt cttcctcgcg acgaggccag gttgctggtg ctttacacga
     2641 acgaccagtc tcttgcccat cgctcgatca aggaattgcc ggagctgtta aatcccggcg
     2701 atctgctggt tgtcaacgac acgaaagtcc gtgcagcccg tgtgacggga tggaaacatc
     2761 cgtccggggg ctccatagag atgttgtttg taagggaact gtccgatcac acgtgggaag
     2821 tgatggtcaa aggcaaattg acgccgggac agagtatcga ggtcagccca gatgcacatg
     2881 tgatcgtcct cggacgtgaa gctgaccgga ccacggttcg aattgagacg gcacgggcta
     2941 ttccagagtt cttcaggttg tatggccgca tgccattgcc accctacatt aagaggctgc
     3001 caacagacga agatcgtgat tggtatcaga cggtctttgc caaagacgaa ggtgccattg
     3061 cggctcccac agcaggactt catttcacac ccgacctgct cgggcgattg aagagtcgtg
     3121 gcgtaggatt gacaaccgtg acgctgcacg tcggacctgg aaccttcaag ccggtgacag
     3181 tggaccggat cgaagaccat cggatgggtc gcgaatggat tgacgtgggg cctgaggcgg
     3241 tacaggccat cgagcgaacc aagtcgaagg gaggaagagt gatcgctgtg ggaaccacgg
     3301 tcgtccgtgc cctagaaact gcggctcaaa ctcaggggca gattcgaccg tatcgaggag
     3361 agaccagctt gttcattact cctggattcc cgttcaaggt cgtgaacgct ttgctcacca
     3421 actttcacct gccgcgcacc acgcttttga tgctcgtctc tgctctggca ggaagaccac
     3481 tgcttctgca ggcctatgaa gaggcagtcc gtgcccgcta tcggttctac agctacgggg
     3541 atgccatgct ggtgatcgaa tagggggtcg tatcgttgcg tcggaaggat actgcgaaag
     3601 acgggacgaa gtgcttacaa cagctcccga tgtatcgtga tgggaagtct cgtctttagt
     3661 gcctcggcat acgccatcag cgcgcgttgc tgtttctgaa acaacatgtc ttgcactgca
     3721 cggtcctttg cagccgcggc tttggcaggc tcggattccg gctgccgaag catgagggcc
     3781 tgggcttccg ccaattcgct tggggtcaat gagacggcgt cgcggactac cattttggcc
     3841 ttggtcgtca atatatcctt cgtctccatg gcctcaaaaa ccgagggagt cagatggttg
     3901 gcggccaaga gtcgccggta aatctccgga tcgaaggtgc cgttcttctg gaagtcaggg
     3961 attctcgtga tcacatcgcg gagctcatcg ttagagaccg tcaagcccat ttccttggcc
     4021 acgatgagcc acatgcggtt ttcgacgagc tgttccacca catattgctt gatcgtttcg
     4081 tccttgaatt ctccgggaac tttttctttg tagacccgat aggtgttttc atacgcgcgc
     4141 cggaattcgt cacgcgacac ggtgagatcc ccaaccgatg cgaccacgtt accggatgat
     4201 tcgccaaatc cccaccaacc catccccacg atgaaagtga cggcaataaa tcccatcgcg
     4261 gccgccagca gccagggaaa gcgatcatgg gtatatcgga gaattttgat catgcacgtt
     4321 ctcgcaaaag ttcgctgacg aatcgacgcc tgcggatttt actcagggat attttcaaac
     4381 gcaagagacg acgatctaat catgcagatt tcgaacgcac tgcggacata catgcgggag
     4441 actggttggg cggggctagt gcccagatga caaagaacgg tctttatttg tgctcgatga
     4501 ggcgatttgt tatactttgc cactaattac gaggagagcc cttggcggag gaggtgggca
     4561 ggatccatcc ggccggttct gcggtctatc ccaaggcgca ggttctgaac cggtgtatcg
     4621 ccaagctcat cgactgtttt atcgttggtg cggcgagtga aatactgact ccgatcggat
     4681 tcctcgccgg cttggcctat gtcctggtgg ccgatggatt tgccggcggg cagagtatcg
     4741 ggaagcggct gatcggtctc caaacgatcc tccccggcac acgcgaaaca gcaggatttc
     4801 gggaatctat catccgcaat ctcccgtttg ccgccgcgca ggtggcgttc gcggtgccgt
     4861 atgtaggttg gatcgcatcg gtggcgattc tggcgtttga aactgtgttg gtggttggga
     4921 atgaacaggg acggcgggtc ggtgatgaag ttgccggtac tcaggtcgtc gatgctggtc
     4981 ggctggcgct gcctgattga cctggacagt aattgggcgg attgaaacgg aagggagatt
     5041 gatcgtgggg tttgcgagcg acattttcgg atggttctcc aacgatctgg cgatcgacct
     5101 ggggacggcg accacactgg tttatgtcca cgggaaagga atcgtgttaa acgagccgtc
     5161 ggtcgtcgcc gtcgagaaaa aaaccgaaaa ggtgctcgcc gttggcgccg atgccaagaa
     5221 aatgttgggc cgtacgccag gcaacatcgt tgcggtccga ccgatgaagg aaggcgtaat
     5281 cgccgacttc gagatggcgg agcaaatgtt aaagcgattt attcaaaagg cccacaatcg
     5341 gagcgcgttc gtacggcccc gcattatcat cggtgtgcct tctcgaatca ctcaggtgga
     5401 acagcgtgcg gtccgagatt ccgcggaact ggctggtgct cgagaggtct atctcattga
     5461 agagccggtc gctgcggcga tcggggcggg gctcccgatt accgagccgt cggggaatat
     5521 ggtcgtcgac gtcggcggcg gcaccacgga tatcgcggtc atctccctcg gcgggattgt
     5581 ctatagcgaa tccgttaagg tggcgggcga tcgcatggac gatgcgatca tgaactacat
     5641 taagaaaaaa tataatctcc tcatcggcga acatatggcg gagcggatca agttcgaaat
     5701 tggatctgct tatccgttcg aagaacgcaa gacgatgatg attaaaggcc gcgacctgat
     5761 ctccggcatt ccacggacgc tggtgatcga cgacgcggag gttcgagaag cgttgcagga
     5821 acccatcgga actatcgtca acgcaatcaa ggtggcgttg gaaaataccc cacctgagtt
     5881 ggcgggagat atcattgatc gtggcattgt gctgacgggc ggcggatcgt tgctgaaagg
     5941 catggacact cgatttcgag aggaaacgaa cctgccgatt attacggtcg acgatccctt
     6001 aacctctgtc gtgttggggg tcggtaagat cctcgatgag ctcgatctgc tcgccaaagt
     6061 ctcggtgatg tctcaagcca acaccttccg gtaaggttcc atccttcatg tggatggtca
     6121 attttcgctc gccctctggg accagacgcc tggtattggc gtcgatcgcc ttactattgg
     6181 tggccctctt cctgctcccc agccaaagcc aaggactgtt acaatacgtc agcagtccac
     6241 ttggcgaagt gttgagcctc ccattgcagg taatcagctc gattgatcac gggatctccg
     6301 aactctggtc cggctatgtc gcgctgcaag gggttagaga agaaaacgaa cagctgcgaa
     6361 aggacatgga cctcctccga gggcagaata gtcagcttcg cgaagccgct gcggctactg
     6421 agcggttgaa gtctcttcta cagttcaagg agcaggcacc gtctgcgata gtcgcggcgc
     6481 aggtgatcgg acgagacgcg actaactcgt atcgctccgt gatcatcaat aaaggtgaaa
     6541 gcgacggcat caggtccgac atgggtgtcg tgacaccggt tggggtcgtc ggtcgcgtcg
     6601 taaaaaccac gggggcgacg gcggtcgtgt tgttggtcac cgatcccaac aatgctatcg
     6661 ccggcctcat ccagcgcacg agagacgaag gaattgtgga gggcactcat cagggactgg
     6721 ccagattgaa atacattccg ttgctctcga ccgtccgcaa tggagatcgt gttgtgacat
     6781 ctggtctcgt cggagggttc ccgcgaggat tgccgatcgg tacgatcacg gggatcgaca
     6841 gagcggaagg cgcgctcttt caatccgcgg aattgatgcc tgacgttgac atggggcgtc
     6901 tcgaagaggt gctcgtgatt caggatccgc gcggccagtc ctccaatggg ggttgaagga
     6961 gctaggtgag ttccgggcca tcacgatgaa tatcttgatt tatatcggtt tgttcttgct
     7021 cctgattcca ctccaaacga cactgcttcc ccatgtgact gtctgggaag tcaaaccgga
     7081 tatgggactc gtggccgcct gctttgtcgg attgttcgca ggtgaactgc ggggagtggt
     7141 tgtcggactt gcgatcgggt gggcattgaa tctcttttcg gccggtgaat tgtggatcag
     7201 ccttgtgacg aaggcagggg ctggattctg tgccggaatg atggggcggc atctcacata
     7261 tgtgtcgccg acgcttatgg gaattggagt atttgccgtg tcatgcctca gcgggctcgt
     7321 ggaactcctc actttcacat ccctttctga gtcatggtgg cgggtgtggt cgttcatgct
     7381 tccacaggcc tgttatgacg ctgttgttgc ggcaggattg tattggattg tttccgagtg
     7441 gttcgtgaga gagcgattca gcatggcatc ccggtactga gcgatggcgc gagatcaata
     7501 acgggccgaa tgatgacgag catcggattt cacgattctg aattgggaga agtgcaacga
     7561 cgattggccg tgttgcgaat cggcctgttg ctcgtcgtcg ccttgttggc gttccggctt
     7621 tggcacctgc agatccgaga aggcccctat tatcgagacc tgtcggaaaa caatcgcacc
     7681 cgctcggtca cgctggagcc ggctcgtggt ctcatctacg atcggcaggg tgtcttgttg
     7741 gcgaacaacg ttccgagttt cagtctctac gtatcgcttg aagatgtgaa agatcgcgag
     7801 gccttaatca acacgctgac aaacctcatc ggattagatc ccgcagtcgt acagaaaaaa
     7861 ctggccggtc gtggtggaaa acagcttcct cggaaaatca aggaccgact cacgcttcgc
     7921 gaagccacgc taatcgaatc tcatcggttg gacttgcccg gggtcatgat ccaagtcgaa
     7981 tcgcaacgta attatcctgg cggagtcacg gccgcgcatc tcctggggta tgtcggcgaa
     8041 gtgtcggctg aacaattgga gaagccggaa tacgccgatc tccatcaagg gagtatcgtc
     8101 ggccaatatg gggttgaaaa attttttgat cgtcacgtac gcgggctggc cggtctgaaa
     8161 agcgtcgagg tcgatgcatt gggccatgaa aaacggacgg tcgtcgttga ccaaccgcag
     8221 gccgggaacg atttgtatct gacggtcgac gtcagactcc aggctttagc cgagaagctc
     8281 ttgggagagg aatccggcgc cattgtggca cttgatccga ccaacgggga tattctcgcg
     8341 atggccagcc ggccggcatt tgacccgaat gtcctctccc gagaactgac gccaaaacag
     8401 tgggtggaaa tcgttcaaga tgaaggtcgt ccgttgaaca atcgggcttc ccaaggacag
     8461 tatccccccg gatcgacttt taaaatcctc atggcggcgg cggcattgga atcgaaaacc
     8521 gtaagtcctt cgagcaccgt acagtgcact ggcgggtatc agttcgggaa acgtgtctat
     8581 cgagattgga aagccggggg tcatggatcg gtcaaccttc atcaggcctt ggtccactcg
     8641 tgcgatgtct atttctacac agtggggcaa cggatgggca ttgataccat cgcctcctat
     8701 gcacaccagt tcgggttggg cgaagaaacc ggtgtcgaac tgccctctga gcgagtcggc
     8761 atcgttccct ccacggaatg gaagcaaaaa gcgaagcatg agccgtggtt gccgggagaa
     8821 acgatctccg cctccatcgg gcagggctat gtgaccgtca cgccgttgca gatggcgagc
     8881 ttgatcggca cagtggcgaa cgacggtgta atgtatcggc cccggttggt tcagggaatc
     8941 atggaccgga cgacgagcca gttgcagcaa ctgccatcca cgccgaaacg gaaactgccg
     9001 gtcaggtcgc aagcgctcga actcattcaa gatgccttag cgagtgtggt caaggagggc
     9061 acaggaacca gggcgaagtc ttccatcgtg acgattgcgg gaaaaaccgg gacagcccaa
     9121 acagcagcac tccggaccgg tcccgagaag gacattccca agaagtttcg tgaccatgcc
     9181 tggttcgtcg cattcgcgcc agtggaatcc cccaaaattg ctgtcgccgt gttggcagaa
     9241 cacatgggcc acggcggatc ggccgcggcg ccgctggcca aagaattgat cgaactgtac
     9301 gcaaagctgg cgcctcaaac gccggctctg acggcaaagt tggagcctgt ggctgccgtt
     9361 tccgttgacg gggtcgtgac ccgatgattg accgtatgat ggacacacga ggagtcgaca
     9421 atttcgacat ccgcttcatc gccctcgtgt ttctaatttt gtcgattggg gtgctatcaa
     9481 tcttcagcgt gacgcatgac cagacagggc cggggctccc gttttttgcc aaacagatca
     9541 tctggattct actgggcaca gtcgcctttt tgatcatgct ggtatgggac tatcatcggg
     9601 ttgcccgctt ggcttatcct gcctatgcga tcatcctcgt cttactggcg gtggtgctgg
     9661 tcgaagggaa aacaagtcgg ggagcacagc ggtggattcc gatcgggcca tttgcatttc
     9721 aaccgtcgga gtttgccaaa ctgatcctga ttctcgtgct ggcccactat tactccaaag
     9781 tgcctcgagt cggctggtta caacgtgtgg tagtgccggg tctattgatg ctgccgggac
     9841 tgttgcttat tctgaaacag ccggacctgg ggagcgggct cagttttctg gcggtctatg
     9901 cagcgatgct gttgatagtt gggatgcggt cccaggcact gggcgtcatt ctcttgtttt
     9961 cgctgatgtt gtttcccttc gcctgggaag taatgtgggg ctccttgcat gattatcagc
    10021 gtcagcgcat tatggccttc gtcgatccgg cctatgaccc tggaggcaag ggctatcatg
    10081 cgttgcaatc cagaattgcc atcggcgcag gcgagctgtt cggaaaaggt ctctacggag
    10141 ggacgcagag ccaattaaag tttctgcccg aaggccatac cgatttcgtt ttttcggtct
    10201 tcgcagaaga atggggcttt ctcggagtgc taggcttgtt gatcttgttc gtggcgctca
    10261 tctggttgtc attggagatt gcggccaaag caaaggatca attgggggcc ttgcttgcgg
    10321 tcggcatcat ttgcatgttg tgtttctgcg tggtggtgaa catcggcatg accgccggta
    10381 tgttcccgat tgtcggaatc ccgctcccgc tgatgagtta tggcggcagt gcgaccatca
    10441 tgacaatggc ctcattgggg ctgttactga atgtcaaacg gcgacgctta agtttgtttt
    10501 attgaatgtg gtgtcaggat tctcgtgaga ggagccgaca caaccagaca tttttattat
    10561 cgaagggacg attgtctcga cgtatcttaa caagtcacgc tgaacctggg gaaatcccgc
    10621 gtttggcagg cacgtcgcct cagtcgtcct tctgaatgga aggagtagag tatgggtgtg
    10681 gaaatagcga tttcggtcgc acgggaagaa acccgtgtgg ctgtcatgga cggcggagtg
    10741 gtaacggatc tctttggaga tcgcgccaag caaaaagact tcgttgggaa tgtatacaag
    10801 ggcaaggtgg caaaagtgtt gccgggcatg caggcagcat ttgtggatat cggtctcgac
    10861 aaggccgcct ttctgcatgt ctcggatttg tcgctggatt ccgagccggg cgatacactg
    10921 gtggatgcag atgaagatga cgacaaggac tcggatatgc tgcgtccccg ccggcaaagc
    10981 tcgaaaccaa tcgagcaatt gttgagcgaa gggcaagagc tgatggtcca aatttcgaaa
    11041 ggaccgatcg gcacaaaagg gcctcgggtg acgacctacg tctcgttccc ggggcggtac
    11101 ctcgtcttca tgccgaatgt ggagcatatc ggagtgtcca gacggattgc ccgagacgaa
    11161 gaacgggccc gccttaaaga catcatgaaa cgagtacgtc atccagggtg tggctacatt
    11221 gtgcggacgg tgagtgaagg ggtcaaggaa gacgagttgc gatccgacgt cgaattcttg
    11281 cacgtgctct ggcaggacat tctgaccaag cgggcgcagt tggctgcgcc cgcgttgctc
    11341 catgctgact tgaatctgag ctttcgagtc gtgcgcgatc tgtttggaaa gaaggtcgag
    11401 cgtttatgga tcgattctcg gcaggagtat gaggcggtac gtgatttcgt tcaacggttc
    11461 tcaccggaac agacttctcg gatccactac tacgataagg aggagagcct gttcgatcat
    11521 ctcggtgtcg aacaagaact cacgcgggcc ctcagtcgaa aggtgtggct gaaatcaggc
    11581 ggatacctcg tgattgatca taccgaagcc atgacggtga tcgacgtcaa taccggccgg
    11641 tttgtcgga
//
LOCUS       DATDNW010000086         8486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_829311, whole genome shotgun sequence.
ACCESSION   DATDNW010000086 DATDNW010000000
VERSION     DATDNW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829311"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VI359_02230"
     CDS             complement(<1..1251)
                     /locus_tag="VI359_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY6973101.1"
                     /translation="MMKHMRLNVIFTFVFVMGFMPVIGGLAIASGLADRVIEHKLANG
                     LTLLMVERHQTPVVSINMTFGVGGVNEQIGQTGLAHLYEHMAFKGTRTVGTKNYEKER
                     PILDELSRVGTELEERQRELARRTTDGPASPEETAAIEALQKRFTALQEQAAQFVMGN
                     EIALLYQRHGGIGLNASTGKDLTRYMISLPSNRLPLWAALESDRMANPVLREFYKERG
                     VVMEERRLRNDDSANGLLFETFTSAAFRAHPYGIPTIGWESDILSLTPGEAEAFFKTY
                     YGPGRATIAIVGDINPKEVIALIERTFGTIPAAPPPPQIVTVEPPQRGERRVEVEFDA
                     EPSVAIGYHKPALGHADDYVFDVIDAVLSDGLTSRLYTKLVREKRLAASIGSDANYPG
                     VRAPNLFILTATPLAPHTTAEVEAA"
     gene            complement(1248..1895)
                     /locus_tag="VI359_02235"
     CDS             complement(1248..1895)
                     /locus_tag="VI359_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6973102.1"
                     /translation="MAKTSVDLLIFDLDGTLIESKWDIAKSVNLTLQELGLPQRPLEE
                     IFGFVGDGVKKLLRSAVGDNNRVSFEEALRVFRGHYLDHCLDRTIYYPGVEQALIHFG
                     KKRKAVATNKSIEYTTVILQGLGGNHFQCVVGGDNGFGLKPEPGMLLHVMERLNVPKE
                     RTVLVGDSTNDINGGHNAGIRVCAVGYGMGNREKMVACQPDWFIERPEELMELFV"
     gene            complement(1895..2500)
                     /locus_tag="VI359_02240"
     CDS             complement(1895..2500)
                     /locus_tag="VI359_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973103.1"
                     /translation="MSESGSVVPAKDEDEGEIEVGRNASIILAWLAVLVMTPTLGSAQ
                     TGSLSNSPTEVVKRYLGLDIKGARLESMSVDALQPYISWKEEPLWSRAVVTQDFMVAE
                     DYRQWEVVDRMEVVIPVTFHVMGLVYFETAGFVSKPETEEVRFRVKAIKNRWKIVDPL
                     LPPHVGQKRIVNFVREAWIKETDSEKRAALATLQEELRKAK"
     gene            complement(2497..3948)
                     /gene="pyk"
                     /locus_tag="VI359_02245"
     CDS             complement(2497..3948)
                     /gene="pyk"
                     /locus_tag="VI359_02245"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY6973104.1"
                     /translation="MRKAKIVCTIGPASDSLALLDRLIEHGMDAARLNFSHGTHESHS
                     RAIKTIREAAARRQVAVAIIQDLQGPRIRVGDLGDGGIELTTGRSVRLQTVLARSGGQ
                     LGASSVAPSGVPEIPVTYQYLVRDVQPGARILIDDGLIELLANRISGGVVECTVVTGG
                     RITSHKGINLPGTCVSAPTLTEKDREDIRFGVAAGVDYLALSFVRGPEDVVAAKQVVA
                     ECNGDLPIIAKIERAEAVGTLNAILDQVDGVMIARGDLGVEMGPEAVPILQKRIIAEA
                     NRRRRLVITATQMLESMTQRTSPTRAEASDVANAVFDGTDAVMLSAETAIGQHPVETV
                     RVMDRIIRAAEEGIEPASTQRPDADHGEVSCSEAICTAAFTAAAAITANAIVAFSELG
                     LTARLISKQRPAAPIIAFTPFESIRQRMALYWGVIPHTMQQIKQTDERVDEAERRLKA
                     EGLVKTGQRIVILSGTRIGQPGRTNLMKLHEVG"
     gene            4141..6456
                     /locus_tag="VI359_02250"
     CDS             4141..6456
                     /locus_tag="VI359_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY6973105.1"
                     /translation="MGSETGRLDFVTIDGLLAYGAALARRQPSEDTLLPASPPPSIDR
                     VRQVRGAMEQIRGFVQRAQTGFPGAEAYRSARKTILEESCAGDDLVFFAAWNRVLAEG
                     ELLPLLHAPIGSVLKPTHRRPVAIVPRAHLTPQLAEGRIVLDLGDDRFWLLPRDLAGR
                     TLLFTMRHGISRVESKTHRVGCRLMNQLDPERGIPRANAVGAALARMVGVVAQQLDFL
                     HLSNYLDPRTFLHFVSRSPNTRQLYHHVIDALVPESVTPPEPLLEAALESSDFGWVTG
                     MEKTMEAEEAAKAFGVDIKIAKRLLKDPLYCYPGGNSFFDLYVDVIDGLHRLGRSHPG
                     TVACLYTHSSTLRALMIYLDPRPFHEAFTEFSDYKESQDNVVLLTYEQGRLSGYSTAV
                     GLSERERIAREAWITVERERKDRVTLKPRQIKRIIALVSGGDFAGAGAALKELRVTGN
                     RLGLEVYFVRHGFLGLANNWIEAVREDDTRGMSSLASSPIGSSRFEDFKEEQVQQAAM
                     RHLEPYRKDSALIVLGGDGSLRGARAIYESFGVQVAGIPGTIDNNIDGTTSLGFHSAV
                     ALANQSIESLKATSSAMGSIFFVEVMGAGSGHLALACAYQARAEGVLVNEHPDPDAYI
                     DEVILGTLKRTLGVPNKSHLFVVAERTPHRHHREGGVHGLVDYVAATIAQWPQRQAKG
                     DHYPLTLATKATILGHTLRGAPPTPEDKTIAQHLAYETVRRLVEDPETIVGCMVAYRD
                     QATIQMIPLHAVTAKPFNWELFARMHGSELS"
     gene            6602..7060
                     /locus_tag="VI359_02255"
     CDS             6602..7060
                     /locus_tag="VI359_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEY6973106.1"
                     /translation="MICLSARFVPPVFLVLALLASSTGSSVAQINNPPATTAQDSDAF
                     GVRTSDPAPSAASEVQIPASLRTGEPPQKARDLLHEIQERQGEPPPGYVGGRTFHNRE
                     RHLPKGRYREYDVNPKLRGRPRDEERIVIEQNTGKAYYTGDHYRTFFSLN"
     gene            7060..7602
                     /locus_tag="VI359_02260"
     CDS             7060..7602
                     /locus_tag="VI359_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY6973107.1"
                     /translation="MRTPSSLSKHLQSTRAPWTSLLVLKSRDSIESLVKPPPGFVLKS
                     IKGAKCKTPATLFAEFAKVMELPDYFGHNWDALEECLADLEWMPAKGYVLLLTDAEQI
                     LSQDEEDYATLLEVLSDAGEAWASGQAGMGERPVTPFHVLFAVTERGKTARAHWGMQE
                     ITASEDREPTKPIRTSRRRT"
     gene            complement(7770..8012)
                     /locus_tag="VI359_02265"
     CDS             complement(7770..8012)
                     /locus_tag="VI359_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973108.1"
                     /translation="MIIVCSWCRKEGNNEFLGEKIPFDDERETHGICVIHYREVRTRW
                     QEVVRAPGSLNAHLSSRNVVLSTLRRWKGLLHLARN"
     gene            8276..>8486
                     /locus_tag="VI359_02270"
     CDS             8276..>8486
                     /locus_tag="VI359_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6973109.1"
                     /translation="MKTIRSAKLFAHAQQLIPGGVNSPVRAFRSVGGQPRFIQRAKGA
                     RLYDVDGNSYLDYVLSWGPMILGHAP"
BASE COUNT         1784 a         2558 c         2358 g         1786 t
ORIGIN      
        1 cgcggcctcg acctccgctg tcgtatgggg cgccagcggc gtggccgtga gaatgaagag
       61 gttcggtgca cgcacaccgg ggtagttcgc atccgaaccg atcgaggctg ccagccgttt
      121 ctcgcgcacg agcttggtat acaaccgcga cgtgagcccg tcgctcaaga cggcgtcgat
      181 cacgtcaaac acataatcgt cggcatggcc gagcgccggc ttatgataac cgatcgccac
      241 tgacggctcg gcatcgaatt ccacctcgac gcgacgctcg ccccgttgcg gcggttcgac
      301 ggtgacgatt tggggcggag gaggcgcagc gggaatcgta ccgaacgtgc gttcgatcaa
      361 agcgatgacc tctttcggat tgatatcacc cacaatggcg atcgtggccc ggccgggacc
      421 ataataggtc ttgaagaatg cctcggcctc acccggcgtc aacgagagga tgtccgattc
      481 ccagccgatc gtgggaatcc cgtacggatg cgcgcgaaaa gccgccgatg tgaatgtttc
      541 aaacagcagg ccgttcgcac tgtcgtcgtt gcgcaaccgc cgttcttcca tgacgacgcc
      601 ccgttccttg taaaattccc gaaggaccgg gttcgccatg cgatccgact caagtgcggc
      661 ccagaggggc agacgattcg acggaaggct gatcatgtag cgcgtgaggt ctttgccggt
      721 cgaagcattg agcccgatcc cgccatggcg ctgatagagc aacgcgattt cgttgcccat
      781 cacaaactgc gcggcctgtt cctgtaacgc tgtgaaccgt ttttgaagcg cctcgatggc
      841 tgcggtttcc tcagggctcg ccggcccatc cgtggttctc ctggccagtt cgcgctgccg
      901 ttcctcgagt tcggtgccga ctcgactcag ctcgtcaagt atcgggcgct ctttttcata
      961 attcttggtc ccgaccgtcc gcgtaccttt gaacgccatg tgttcataga gatgcgcgag
     1021 accggtttgg ccaatctgct cgttcacgcc tccgaccccg aaggtcatat tgatcgagac
     1081 gaccggtgtc tggtgccgtt caaccatgag gagggtcaag ccgttggcca gtttgtgttc
     1141 aatcacgcga tcggcgagac ctgaagcaat tgccaatcca ccgatcacgg gcatgaagcc
     1201 catgacgaac acgaacgtga agataacgtt gaggcgcatg tgtttcatca tacgaagagc
     1261 tccatcagtt cctccggtcg ttcaataaac cagtctggtt ggcaggcaac catcttctcc
     1321 cgattgccca tgccatatcc caccgcacaa actcggattc ccgcattgtg gcccccattg
     1381 atgtcgttgg tgctgtctcc taccaggacg gtccgctcct tgggcacgtt caaccgctcc
     1441 atcacgtgca acaacatccc aggctccggc ttcaaaccaa acccgttgtc gccgccgacg
     1501 acacactgga aatgattgcc accgagtcct tgcagtatga ccgtggtgta ctcgatcgat
     1561 ttattggtgg ccacggcctt acgcttcttt ccgaaatgga tgagtgcctg ttcgacgccc
     1621 ggataataga tcgtgcgatc cagacaatgg tctaaataat gcccgcgaaa tacgcgcagc
     1681 gcctcttcga aactgacgcg attattgtcg ccgacggccg agcgcaacag cttcttgacc
     1741 ccgtcgccta caaaaccgaa aatctcctcc aacggccgtt gcggaagccc cagctcttga
     1801 agcgtgaggt tcaccgattt ggcgatgtcc catttggact cgatgagcgt cccgtccaaa
     1861 tcgaaaatga gcagatcaac cgaggtcttc gccattattt cgcctttcgc agttcctctt
     1921 gtaaggtcgc aagcgcggcc cgcttctccg aatcggtttc tttaatccag gcttcccgca
     1981 caaaattcac tatgcgcttc tggccgacat ggggtggaag caagggatcc acaattttcc
     2041 aacgattctt gatggctttc acgcgaaaac gaacttcttc cgtctcgggc ttcgacacaa
     2101 atccggccgt ctcgaagtag accaacccca ttacgtggaa ggtgacggga atgacgactt
     2161 ccatccggtc gacgacttcc cactgacggt aatcctcagc caccataaag tcctgtgtta
     2221 cgaccgcccg gctccagaga ggctcttcct tccaactgat atacggctgc agggcgtcga
     2281 cggacatcga ctcgaggcga gcccctttta tgtccaatcc cagatagcgt ttcacgacct
     2341 ctgtcgggga attgctcaac gatccggttt gggccgaccc cagagtcgga gtcataacca
     2401 ggactgcgag ccaggccaga atgatggatg cgtttcgtcc cacctctatc tccccttcgt
     2461 cttcgtcttt tgcgggaacg actgaaccgc tctcgctcat cctacttcat gcagcttcat
     2521 taagttcgtg cgccccggct ggccaatgcg agttccggac agaatgacga tccgttgacc
     2581 ggtcttcaca agtccttctg ctttcaaacg acgttctgcc tcatcaacac gttcatctgt
     2641 ttgcttgatc tgttgcatgg tgtggggaat caccccccaa tagagcgcca tgcgctgccg
     2701 gatcgactca aacggtgtga atgcgatgat cggagcagcg ggacgctgtt tggagatcaa
     2761 gcgagccgtc agtccgagtt cgctgaatgc gacgatggcg ttggccgtga tggccgcggc
     2821 cgcggtaaaa gccgccgtgc aaatcgcttc agaacaggag acctcgccgt ggtcggcatc
     2881 aggccgttgc gtcgaggccg gctcgatccc ttcttccgcc gctcgaatga tccgatccat
     2941 gacacgaacc gtttcgaccg ggtgctgacc gatcgctgtc tcagccgaga gcatcacggc
     3001 gtcggttcca tcgaacacgg cattggcgac atccgacgct tccgctctgg tcggactcgt
     3061 gcgctgtgtc atggactcca acatttgcgt cgcggtgatg acaagacggc ggcggcggtt
     3121 ggcctccgcg atgatgcgct tctgcaaaat cggcaccgct tcggggccca tctccacgcc
     3181 cagatcgccg cgcgcaatca ttacaccgtc cacctgatcc agaatggcgt tcagcgtgcc
     3241 cactgcttcg gctcgctcaa tttttgcgat gatcggtaga tcgccgttgc attcggcaac
     3301 gacctgtttt gccgcgacca catcctccgg accacgaaca aacgacaggg cgaggtagtc
     3361 gacgcctgcc gctacgccaa atcgaatgtc ctctcgatcc ttctcagtca gggtgggagc
     3421 gctgacgcac gtgccgggga gattgatgcc cttatgtgac gtgatccttc ctcccgtgac
     3481 gaccgtgcat tcgacgacac cgccggagat gcgattcgcc agcaactcga tgagtccgtc
     3541 atcaatcaga atccgggcgc cgggttgaac atcacgcacg aggtactgat aggttacggg
     3601 aatctccgga acgcccgatg gagctacgga ggaggcgccg agctgtcctc cggaccgcgc
     3661 cagcacggtc tgcaatcgca cggatcggcc ggtcgtcagt tcgattccgc catcaccgag
     3721 atcgccgacg cggattcgtg gcccttgcaa atcttggatg atggcaactg ccacctgtct
     3781 gcgagccgca gcttcacgga tcgttttaat tgcgcggctg tgagattcgt gagtaccatg
     3841 agagaagttc agtcgagccg catccatgcc atgctcgatg agccgatcca gcaacgccag
     3901 cgaatcactg gcgggaccga tcgtgcacac gatcttcgct tttctcatgt tgccttcttc
     3961 ttgcttgaca agacgggagc gggcacccaa tcattgtggg ccagcgccgg caggaaccgg
     4021 ggatcagcgt ggctgagaaa ccattcatcc tcgcgttctg tgcacggaag ccattctaac
     4081 aaacgcgaat tctggtcgca aaggttcttc gagacgtcga caagcgtcga aggggtagaa
     4141 atgggaagcg aaacgggacg acttgatttc gtgacgatcg acggcttatt ggcatacgga
     4201 gccgcgctcg cccgacgcca accgagcgag gacacgctcc tgcctgcatc gcctcctcca
     4261 tcaatcgacc gagttcgcca agtccggggc gcgatggagc agatccgcgg ttttgtccag
     4321 cgcgctcaaa ccgggtttcc cggagccgag gcctaccgat cggcacgaaa gacgatcctc
     4381 gaagaaagtt gcgcgggcga cgacctggta ttttttgccg cctggaaccg agtcctcgcc
     4441 gaaggggaac tgctgccgtt acttcatgct cccattggat cggtgttgaa gccgacccac
     4501 cgtcgcccgg tggcgatcgt cccgcgagcc catcttaccc cgcaattggc cgagggtcgc
     4561 atcgtactgg acctgggcga cgatcggttc tggttgttgc cccgcgattt ggccggccga
     4621 acgttgctct tcacgatgcg ccacgggatt tcccgcgtcg agagcaaaac gcaccgggtc
     4681 ggatgccgct tgatgaacca actcgatccc gagcgaggca tcccgcgagc gaacgctgtc
     4741 ggggccgctt tggcccgtat ggtcggtgtg gtggcccaac aactcgattt tctgcatcta
     4801 tccaactatc ttgatccgcg caccttcctc catttcgtca gccgcagtcc caacacgcgg
     4861 caactctatc accacgtgat cgatgcactc gtccccgaat ccgtgacgcc ccctgaaccg
     4921 ctgctcgaag cggccttgga gtcgtcggat ttcgggtggg tcaccgggat ggaaaaaacg
     4981 atggaggccg aggaagcggc caaggccttc ggcgtggaca tcaagatcgc taaacgtctt
     5041 ctgaaagacc cgctgtactg ctatcccggc ggcaattcgt ttttcgatct gtacgtcgat
     5101 gtgatcgatg gcctccaccg gctgggacga tctcatcccg gtacagtagc ctgtctgtat
     5161 acgcacagct cgaccttgcg ggcgctcatg atctatctgg acccgcgtcc ctttcacgaa
     5221 gccttcactg aattcagcga ctacaaagag agtcaggata acgtcgtcct tctgacgtat
     5281 gaacagggac gcctgtccgg ttattccacg gccgtgggac tgtcggaacg ggaacggatc
     5341 gcgcgcgagg cgtggatcac cgtcgaacgg gaacggaagg atcgggtcac gctgaaacct
     5401 cgccagatca agcggatcat cgccctggtg tctggtggcg attttgccgg cgccggcgcg
     5461 gccttgaagg aactgcgcgt gaccgggaac cggctcggcc tggaggtgta cttcgtccgc
     5521 cacggtttcc tcgggctcgc gaacaattgg atcgaggcag tgagagagga cgatacgcgc
     5581 ggcatgagca gcctggcgag cagcccgatc ggcagcagcc gctttgaaga tttcaaggag
     5641 gaacaggtcc aacaggccgc catgcggcac cttgaaccct acaggaaaga cagcgcgttg
     5701 atcgtgctgg gaggcgacgg cagccttcgc ggggcccgtg cgatctacga aagcttcggc
     5761 gtccaggtcg ccgggatccc cggaaccatc gataacaata tcgacggcac tacctcgctt
     5821 ggctttcatt cagccgtggc gctcgcgaat caatcgatcg aatcgttgaa ggcgaccagc
     5881 tccgcgatgg gcagcatctt tttcgtcgag gtgatgggag ccggatcggg ccatctcgcg
     5941 ttggcctgcg cctatcaggc gagggctgag ggggtgttgg tgaacgaaca ccccgatccc
     6001 gatgcctaca tcgacgaagt tattctggga actctcaaga ggacgttggg cgtacccaat
     6061 aagagccatt tgtttgtcgt ggctgagcgc accccccatc gacaccaccg agagggcggc
     6121 gtccacggcc tggtcgatta tgtggccgcg acgatcgcgc agtggccgca acgtcaggcg
     6181 aagggcgatc actatcccct cacgttggcc accaaggcca ccatcctcgg tcatacgctg
     6241 agaggcgcac cccccacccc ggaagataag acgatcgcgc aacacttagc atacgagacc
     6301 gttcgacgat tggtcgaaga ccccgagacc atcgtcggtt gtatggtggc ctaccgagac
     6361 caggccacga ttcagatgat tccacttcat gctgtcacgg ccaagccatt caactgggaa
     6421 ctcttcgccc ggatgcatgg gagtgaattg tcataagaat aaacggagcc gtggcctcag
     6481 tcagacctgt tgattaagca atccaatttg tggtcgcatc tcccacaagc cattttccgt
     6541 tcgttgacct ccgtttagag cgcgtgatag cgtttcgctc tcaacctcga tcgacccagc
     6601 gatgatttgt ctgtccgcac gatttgtacc gccggtgttc cttgtcctgg cactcctggc
     6661 cagttctacg gggagttcgg tcgcgcagat taacaacccc ccagcaacca cggcacaaga
     6721 ctctgatgcc tttggcgttc gaacaagtga tcctgcacca tccgccgcgt ccgaagtgca
     6781 aatccctgca agtctccgga caggagagcc tccacaaaag gcgagagacc tgttgcatga
     6841 gatacaagaa cgtcaaggcg aaccgcctcc gggatacgtg ggcgggcgta cgttccacaa
     6901 tcgtgaacga cacctcccga agggccgata tcgcgaatat gacgtgaatc caaaattgcg
     6961 cgggcggccg cgggacgagg aacggatcgt gattgaacaa aacaccggca aagcctatta
     7021 caccggcgac cattatcgaa ccttcttctc gctgaactaa tgcgaacacc atcgtctctt
     7081 tcaaaacatc tccaatcgac gagggcaccc tggacctcgc tattggtcct caagtctaga
     7141 gattccattg aatccctcgt caaaccgcct cccggcttcg tgctgaaatc gattaagggc
     7201 gcaaagtgta agaccccggc taccctcttt gccgaattcg ccaaggtgat ggaactgccg
     7261 gactactttg gtcacaattg ggatgcgctc gaggaatgtt tggctgactt ggaatggatg
     7321 ccagcgaaag gctatgtgct gctgctcacc gatgcggaac agatcctatc tcaggacgaa
     7381 gaagactacg ccacgctgct cgaggtgttg agcgatgccg gagaggcctg ggcatcggga
     7441 caggcgggaa tgggcgagcg accggtcaca ccttttcacg tgctgtttgc ggtcaccgaa
     7501 cggggaaaga cggcacgagc ccattggggg atgcaagaga tcacggcctc ggaggatcga
     7561 gagccaacca agccgatacg aacttccaga agacggacgt aacgcgacat cacattaccc
     7621 ccgaccgtgc tgacgcgtct cccaccatta agctcaaacc aggatctatc gaccatccat
     7681 tcaaatagtt tcatcacggt cggtgtccga cgaagagtcg aggagaatta cgcgtggggt
     7741 tacgagaaga tggattcggt ttccagattt tagttcctgg cgagatgtaa gaggcccttc
     7801 cagcgccgca aggtcgacag cacgacattg cggctgctga gatgagcatt taatgaaccc
     7861 ggtgcgcgaa ccacttcctg ccaccgcgta cggacttcgc gataatggat gacacagatc
     7921 ccgtgcgtct cgcgctcgtc atcgaacgga atcttctctc cgagaaactc gttgtttcct
     7981 tccttacggc accaggaaca gacgatgatc atcctcgatc ctttcaatac atgaataagc
     8041 tccattacac cacgcaagag tcgggccgca gtctgtggat aatctgttca cagaagtatc
     8101 gagagattgc aatgctttcg atgcttagga atccactttc ctgtatcaaa aaggatagaa
     8161 gtgaatctga tcagacactt tttgacctca cctgaggcgt tcgtcctcac gttcagttcc
     8221 ctccttcgac tttcttgacc ccttctctag ctgcgtggta gggtgcgccc ccgtcatgaa
     8281 aactattcgc tccgccaaac tttttgcgca cgcgcaacaa ttgatccccg gaggggtgaa
     8341 cagcccggtt cgagcgtttc ggtctgtcgg tggacaaccg cgtttcatcc aacgagccaa
     8401 gggtgcgcgc ctctacgacg tcgacggaaa cagctacctc gattacgtcc tgtcatgggg
     8461 accgatgatc ctgggccatg ccccga
//
LOCUS       DATDNW010000087         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_898055, whole genome shotgun sequence.
ACCESSION   DATDNW010000087 DATDNW010000000
VERSION     DATDNW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898055"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VI359_02275"
     CDS             complement(<1..884)
                     /locus_tag="VI359_02275"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY6973110.1"
                     /translation="MLAGFGLVFAGIVVISIASYRNTSVLIKNSRLDTRSHELVQLLS
                     SIGEALDDAERGHRRYLVTGDEAYLKGHKTVLEQMPDFSRYLRGLTEGSPDQQERVAS
                     LERLIGQQLDAEAAAISARETNGYEGVRRIALSGAAKQELDAIHRLIVELETTEQKAL
                     RSRVIQSTGSTRNTIVLLVLGALLQLVLLASVYYLIHHDVTERRRVAAELRRRGELLQ
                     AANKELEAFSYSVSHDLRAPLRHIDGYASLLSKTAGESLNDKARRYLQTISDSAKQMG
                     QLIDDLLVFSRMGRQEMLR"
     gene            complement(1161..>2164)
                     /locus_tag="VI359_02280"
     CDS             complement(1161..>2164)
                     /locus_tag="VI359_02280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY6973111.1"
                     /translation="LKFFILGVFAAGLLAYGISLVYGETGKLVFSDMTSAQATPGLII
                     GYLLIFAALGFKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKGAAFAILLRMFYVALA
                     SFKPTWVLMLVAASILSMTYGNIVAIAQKNIKRLLAYSGIAQIGNVLIGLAAGTKMGS
                     DAILFYLLTYLFANLGAFAIVIAVSQAIGSDEIEDYSGLGRRSPFLAFAMLIFLLSLA
                     GVPPLAGFIGKIYIFVAAIKEGLYTLITVGLINIVISMYYYLIVVKKMYIIEPHDPSP
                     VSISGPMKAVVYIGLAGTLAIGIYPQPVIDWVVAATLMFSNIAGPAAFIPPPSPFGG"
BASE COUNT          485 a          579 c          663 g          437 t
ORIGIN      
        1 cgcaacatct cttgccgacc catgcgggaa aataccagca ggtcgtcgat gagctggccc
       61 atttgcttgg cggagtcgga gatcgtctgg agatagcgtc tggctttgtc gttgagcgac
      121 tccccggccg tcttggatag taaggatgcg tacccgtcga tatgccggag gggcgcgcgc
      181 agatcatgcg agacggaata gctgaaggcc tcgagttcct tgttggccgc ttggagtaac
      241 tctccgcgac ggcgaagttc tgctgcgacg cgacgtcgct ccgtgacatc gtgatggatc
      301 aggtaataca cggaggcgag caacacgagc tgcaggaggg cgccgaggac caggagcacg
      361 atggtgtttc tcgtgctgcc cgtcgattga atgacacggc tccgaagggc cttctgctca
      421 gtcgtctcca gttcgacgat caagcgatgg attgcgtcga gctcttgttt cgctgcgccg
      481 gacagggcga tgcggcgaac cccctcgtag ccgtttgtct ctcgtgccga aatagcagcg
      541 gcctcggcat cgagttgttg accgattaat cgttcaaggc tcgccacccg ttcttgttgg
      601 tcggggctcc cttctgtcag acctcggaga taccgagaga agtcgggcat ctgctcgagg
      661 actgtcttgt gtcctttgag gtaggcctcg tcgccggtca caagatagcg gcgatgcccg
      721 cgctcggcat cgtccaatgc ctcgccgatc gaacttagaa gttgcacgag ttcgtggctt
      781 ctcgtgtcga gccggctgtt cttgatcagc acggatgtgt ttcgatacga cgcgatgctg
      841 atcacgacga ttcccgcaaa cacgagtcca aacccagcga gcacgcgctg ctcaatggac
      901 cagctggcga accatcgcac agctctggtt acgcgcgagg gaggcaggca tgtcttctcg
      961 gggtccgacg ttgatggcac gggcgctgtc ggggcggaaa cggtagggcg gtcttgtgtg
     1021 acggatgcca tgatagatgt agagcccaca ccgaagcgtt atgggaaaac ccgtgacgga
     1081 actgtactct gccccactat accctgcaag aaatatttta ccagaagcct cggtgggagc
     1141 ggcgtgacga agaggaaaga ttatccaccg aaaggtgaag ggggcggaat gaacgccgct
     1201 ggaccggcaa tgttcgagaa catcagcgtt gcggccacta cccagtcgat caccggttgt
     1261 ggatagatgc cgatcgcgag cgtcccggcg agtccgatgt ataccacggc cttcatgggg
     1321 cccgagattg acaccggtga cgggtcgtgg ggctcgatga tgtacatctt cttcacgacg
     1381 atcaggtagt agtacatgga gatgacgatg ttaatcagtc cgaccgtgat gagtgtatag
     1441 agtccttctt tgatcgccgc gacaaaaata tagattttgc cgatgaatcc tgcgagtgga
     1501 ggcacgccgg ccagagacaa cagaaaaatc agcatggcga acgcaagaaa cggcgaccgg
     1561 cggccgagtc cgctgtaatc ctcgatttca tcgctgccga tcgcctggct caccgcgatg
     1621 acgatggcga aggctccgag attcgcgaac aggtaggtca gcaaataaaa taaaatcgca
     1681 tcacttccca tctttgtccc agcggcaagg ccgatgagca cattgccaat ctgagcaatg
     1741 ccggaatagg ccagcagtcg tttgatgttc ttctgcgcga tggccacgat gttgccatag
     1801 gtcatcgaca gaatcgaggc cgccaccagc atgaggaccc aggtgggttt gaagctggcg
     1861 agcgcgacgt agaacatccg aaggagaatg gcgaacgctg cccctttggg tgcaatcgac
     1921 aggaatgcgg tcaccggtgt cggtgagccg tgataggtgt cgggaatcca ggaatggaat
     1981 ggcacagccc cgattttgaa tcctagtgcg gcgaagataa gcaaataacc gatgatcaac
     2041 cccggcgtag cctgtgcaga cgtcatatcc gagaagacga gtttccccgt ttcgccgtag
     2101 accaagctga tcccataggc aagaaggccg gccgcaaaaa cacccaggat gaagaatttg
     2161 agcc
//
LOCUS       DATDNW010000088         3384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_760705, whole genome shotgun sequence.
ACCESSION   DATDNW010000088 DATDNW010000000
VERSION     DATDNW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760705"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="lnt"
                     /locus_tag="VI359_02285"
     CDS             complement(<1..1031)
                     /gene="lnt"
                     /locus_tag="VI359_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY6973112.1"
                     /translation="MDREINPLSLNVGGLHPFPDFVTVRSMILPGTSRTHGLLLAGAS
                     GFLLPFCFPRFDLSLLAWIALIPLHFAMEGKRKSQAFWIGWLAGTVGFTGIMAWVVTV
                     MHTYGKVPLAISYAILLLLAAYLGLYVGLYGAGLVWFRGLIPRYGLLLAPCLWVSLEL
                     LRTYLLSGLPWALLGYSQYQQTDLIQFADHTGVYGVSFVIVLVNVALAELILWLMPLF
                     RGFRPAALPWELMTAAALLLVLAWSYGLWMSTGAPLSDVARATLSVGVVQPNIDQSVK
                     WDKAYREETLRRFEDLTARLDASSDLVVWPEAATPFLFEREPEYQLRLIALANQAQAS
                     LLFGSPALR"
     gene            1107..1730
                     /locus_tag="VI359_02290"
     CDS             1107..1730
                     /locus_tag="VI359_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6973113.1"
                     /translation="MKRWANISFVLSLSVIVLLLVAGCGKKVTSSSGDTASERVKPEP
                     IAAVPPAPGASGGSTTPGGMDAARSTPGSQGQGGLPGGGPGGAGGGMAGGPGGGSELQ
                     DIFFDYDRFTVRADGVQAMEQNARWLRAHKGQNVLIEGHCDERGTQAYNLVLGEKRAR
                     SAKRYLEDLGIAGSQLQITSYGENRPVCKEQTESCYQRNRRAHFVAK"
     gene            1788..2402
                     /locus_tag="VI359_02295"
     CDS             1788..2402
                     /locus_tag="VI359_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973114.1"
                     /translation="MKSNLLIGIWVLCSLVISGCAAQGEKIDVKISTKPSTEAKPPAS
                     AAVKVAVVPFQDDRIDRSRLGTRQHLWGGESTFALPSGTVGEASARALADYLKQKGWQ
                     AALATNGTGADITITGKLLEVAVDAKSGVAHTALTGRNKMVIQADNAADGSKVRETVS
                     GIGSDTVFWFDPDDAEALLNDLYDRNFERFLADTKLDGKVLKLR"
     gene            complement(2495..2803)
                     /locus_tag="VI359_02300"
     CDS             complement(2495..2803)
                     /locus_tag="VI359_02300"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY6973115.1"
                     /translation="MPTYVSLVKFTQHGLQTMKDKGIERAEMVKENAHALGGKLVQAY
                     YCLGEYDVVAVWEFPDNKTAMKAAVLNAAIGHIQITTMPAVGRDEWKKLLQDTLGKKR
                     "
BASE COUNT          789 a          939 c         1004 g          652 t
ORIGIN      
        1 tgacggagcg ccggacttcc aaacagcagc gaggcttgag cctgattcgc cagagcgatc
       61 aatcgcagct gatattccgg ctcccgctca aacaggaacg gagtggcggc ttccggccat
      121 accacgagat cggaggacgc atcgagcctg gccgtcagat cttcgaatcg ccggagagtt
      181 tcctcacggt aggccttatc ccatttcact gactggtcga tattaggttg caccacccca
      241 accgagaggg tagcgcgggc cacatcggat aagggggcgc ccgtcgacat ccagagccca
      301 tagctccatg ccagtaccaa gagtaaggca gcagcagtca tgagctccca gggtaaggcg
      361 gcagggcgaa atcctcggaa cagtggcatg agccatagga tcaattccgc aagcgccaca
      421 ttgaccaaca caatgacgaa cgagacccca tagacgccgg tgtgatcagc gaactgaatc
      481 agatcagtct gctgatattg cgagtagccg agcaatgccc atggcaagcc actcagcaaa
      541 taggtgcgca gcagttccag acttacccag aggcatggcg cgagcagcag gccgtatcga
      601 gggatgagcc cgcgaaacca aaccagaccg gccccataga ggccgacata gaggcctagg
      661 taagctgcca gcaacaacag aatggcatag ctgatggcca acggaacttt cccatacgtg
      721 tgcatgacgg tcacgaccca ggccatgatg ccggtgaagc cgaccgttcc ggccaaccag
      781 ccgatccaga aagcctgaga ttttcgcttg ccctccattg cgaaatggag ggggatcagg
      841 gcgatccatg ccagcaggct gaggtcgaac ctgggaaagc agaagggaag cagaaatccg
      901 ctggcaccgg caagcaacag cccgtgcgtg cgagaggtgc cggggagaat catcgagcgt
      961 accgtaacga aatccggaaa gggatgcaac ccacccacat tgagagaaag tgggttgatt
     1021 tcacggtcca aaaggggtat tccatgttcc ggcaatggtt ttcattgggg cccggtcgag
     1081 aggaccggct aaaggagggg ggacctgtga aacgttgggc aaacatcagt ttcgttctgt
     1141 ctctcagtgt catcgtgcta ctccttgtcg caggctgcgg caagaaagtc acgtcttcgt
     1201 ccggagacac ggcctccgaa cgggtcaagc ctgagcccat cgcggctgta ccaccggcac
     1261 cgggtgcgtc aggcggatcg acgacgccag gagggatgga tgcggctcgg tctacgcctg
     1321 gtagtcaagg gcagggcggg ttacctggcg gcggtcctgg aggagcaggg ggcgggatgg
     1381 caggcggacc aggcggcggg agcgaactcc aagacatctt ctttgattac gatcgtttta
     1441 ccgtgcgcgc tgacggtgtg caggcgatgg aacagaacgc ccggtggctc cgtgcacata
     1501 aaggccaaaa cgtgctcatc gagggccatt gcgatgagcg aggcacacag gcctacaatc
     1561 tggtcctcgg tgaaaagcgt gcccggtcgg cgaaacgcta tctcgaagat ctcggtattg
     1621 ccgggtcaca gttgcagatc acaagctatg gtgaaaacag gccggtgtgc aaagagcaaa
     1681 cagaaagctg ttatcaacgg aaccgccgcg cacatttcgt tgcaaagtga cgctcggctt
     1741 gcccataagt ccgagaaccg tcgtcacgtt atcgtaagga ggagcacgtg aagagcaatt
     1801 tactcatcgg catctgggta ctgtgttcgc tcgtcatcag cggttgcgct gcgcaggggg
     1861 agaagataga cgtcaagatc agcaccaagc ccagtacgga agcgaagccc ccggcatccg
     1921 ctgccgtgaa ggtggctgtt gttccgttcc aagatgaccg gatcgatcgg tcgcggctcg
     1981 ggacccgtca gcacctctgg ggcggggaaa gcacctttgc cttgccaagc ggcacggtgg
     2041 gagaggcaag tgcccgcgct ctggctgatt acttaaaaca gaaaggctgg caagcggctc
     2101 tcgccaccaa cggcaccgga gccgacatca cgattacagg aaaattgctc gaagtcgccg
     2161 tcgatgcgaa gagcggtgtc gctcacactg cccttacggg acggaacaag atggtcattc
     2221 aagccgacaa tgccgccgat ggcagtaaag tccgtgagac cgtctcaggg attggtagcg
     2281 acaccgtatt ctggtttgat cccgatgacg ccgaagcatt gctgaatgac ctctatgaca
     2341 gaaacttcga acggttcctt gcggatacga aactcgacgg gaaggtcctc aaacttcgct
     2401 agggcatagc ttccaatatc gcttccccta cgtgcaactc ggcacgggac cagccgctgg
     2461 tcccgtgccg cagagagaac tgcgcatcac tctattatcg ctttttcccc agagtgtctt
     2521 gcaggagttt tttccattcg tcccgaccaa cggcgggcat agttgtaatc tgaatatggc
     2581 cgatcgccgc gttcaagacg gccgctttca tggcggtctt gttgtcagga aattcccaga
     2641 ccgcgacgac gtcatattcc ccgaggcagt agtaggcctg gaccaacttg ccaccgagcg
     2701 cgtgcgcgtt ttccttcacc atctcggccc gctcaattcc cttatccttc atggtttgaa
     2761 gaccatgctg ggtgaatttc acgaggctga cgtaggttgg cataatgtac ctccttcgtg
     2821 agggtggcgg atcgatggac ggaacgctgt tggcaaattg taggcttctt cgtcagcctc
     2881 ccacaagggg aggccgttga gccgacacca gcgaaagctt gactcgctcg aggtggaggg
     2941 ctatcgtgag tttgtgggag gagaaaacgc cccgagggaa tatggttcat gaggggatcg
     3001 agcgctgagg atggtgtgca ggaccagctt gtactgggaa gcctaaagtc ctcccaagat
     3061 ctccgccgta cctaatcgtc ctcggttagg tgcgtgctcc tcccacccat attttgtctc
     3121 accgcttcat caattcattc agagcacgca attattttca tgccgagggt tcactctact
     3181 gatcaatctg tgtacaatgc cgcgcaattt tatccgcgct cgttcctaag aggatgaatg
     3241 gctagatctc acgctgccgt cgctcattcg aaacccacat caccaatatc cgctagcgaa
     3301 ccggtcgagc cgaagaaggt tcgcgcgaag ctcccgcaag ccgtaacggc tgtggaaatg
     3361 ggcgcgcggc agcgggaaat ttct
//
LOCUS       DATDNW010000089         5430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_422576, whole genome shotgun sequence.
ACCESSION   DATDNW010000089 DATDNW010000000
VERSION     DATDNW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422576"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VI359_02305"
     CDS             <1..948
                     /locus_tag="VI359_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY6973116.1"
                     /translation="LALPRVTIDEPTVQMTFSVNNSPFAGREGKFLTSRHLRERLFKE
                     LETNVSLRVQETDSPDRFLVAGRGELHLGVLIEQMRREGYELQVSQPEVILHRDGDKV
                     TEPYEELTILIPETYQGAVIEEIGKRRGEMRHMKLVHSDAGPSEMHLEYHIPTRGIMG
                     LKNLLLAKTRGTIIMHHVFKAYEPAEERDLVVAPHGSLVAFEDGCSTGYAIFMTQERG
                     AMFIGPGVEVYRGMVVGENSRDEDLDVNVCKEKHLSNMRASGSDEALVLTPPREMTLE
                     YALEYIGPDELVEITPHNLRVRKRLLNPDDRRKARKAGK"
     gene            1167..1718
                     /locus_tag="VI359_02310"
     CDS             1167..1718
                     /locus_tag="VI359_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973117.1"
                     /translation="MPLPATHRDSLKETLAWDHDLIGAVAPSVMPPREMPDTILFAAR
                     LARGLTLLAQGTAFDLTTQQYLNPSDWKDRPLAHFLIADHVSFMQGDDQLGDRSWCYT
                     LGLSKFGMDELEIFFPRGLPDTHARELLGEAASELLRSGHVLKVGSQFRVTLLGQTVT
                     IANYRTAAPAGRTLSFREIKAVS"
     gene            1864..2334
                     /locus_tag="VI359_02315"
     CDS             1864..2334
                     /locus_tag="VI359_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY6973118.1"
                     /translation="MSDVAAEIKVGDTAPDFNLKDQDQKDVKLSDYKGKKNVVLCFYP
                     LDWSPVCQGENKCLTDDFPKFQGANAELFGISCDSFFSHKAWADSLDLKHRLLSDVHR
                     TTAKAYGLYFEPLNCSKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAKLS"
     gene            2420..2752
                     /locus_tag="VI359_02320"
     CDS             2420..2752
                     /locus_tag="VI359_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6973119.1"
                     /translation="MSGIVEDVSDTNYKEFTDAPASIVAYGLATCEPCKTYDPILEEM
                     AAKFPSIKVGKAKMHVPGRCREIKKTHTFETYPTTHFFSKGKLLLAREGVVEPTELAA
                     LIADHLQP"
     gene            2895..4412
                     /locus_tag="VI359_02325"
     CDS             2895..4412
                     /locus_tag="VI359_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973120.1"
                     /translation="MASHSRSRNFPSYDAQVLLNSQPVIVSVIDPQTHQVQFQNETGL
                     KKFGDISGFSCYEKIAGCQTPCSFCRMPEALTTDSVVSNEVALPGDKYLLVHWSKAPT
                     IDGRLHVIETIADITEHKRTEQALHHSQKMEAVGRLAGGIAHDFNNLMMVVIGHAQRL
                     LQQFSSHPSKGELETISQAGMKAAALTKKLLTFGQRQVFEPKEVNINTVIREMEDILK
                     RLIGEHIQTMIVLHPRTGHALVDPIQLEQVIMNLVLNARDAMPDGGLLNVETDNVELD
                     EQYARLHPGSSAGPYVKIMVQDAGCGMDAETLTHIFEPFFTTKEPGKGTGLGLATVYA
                     IVKQSRGYIEVTSEPGRGSRFTVYLPRVGEPAIEAKVPHPDTEVCLIHETILVVEDDE
                     GIRKLITTILQDQGYNILAAGDGIEALQKLQLCKGPCHLVIADVIMPRMKGTAFIEAV
                     QAMRPDARVLYMSGYAGDTLQANGVTANSPFLQKPFLPATLIEKVRELLQTPAPC"
     gene            4917..>5430
                     /locus_tag="VI359_02330"
     CDS             4917..>5430
                     /locus_tag="VI359_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973121.1"
                     /translation="MNATYKVFLASLLVCSLTAVSSLALAAGSGSNQDRDGQHRLLSA
                     ELRAKLERVEDKLKDRREHHHNPLEALQADVASLKSQLAGLVSSNASLSSQLNAANSQ
                     IALLTSQISALQTSGGSGGTTNPALTALAKYVTVDPNPINGVKGPHVIFSGVNLHVRS
                     GSGSTGDNGTP"
BASE COUNT         1337 a         1544 c         1424 g         1125 t
ORIGIN      
        1 cttgcgctgc cccgtgtcac gatcgacgag ccgaccgtac aaatgacctt ttccgtcaac
       61 aacagcccct tcgccggacg cgaaggcaag ttcctcacct cccgtcacct gcgtgaacga
      121 ttgttcaaag aactggagac caacgtctcg ttgcgagtgc aggaaacgga cagccccgat
      181 cgattcctcg tcgcaggtcg aggcgaactc catcttggcg tgctgatcga acagatgcgg
      241 cgcgagggct atgagctcca agtctcgcag ccggaggtca tcctccatcg cgatggcgat
      301 aaagtcaccg agccctacga ggaactgacc atactgattc cggaaaccta tcagggcgcc
      361 gtgatcgagg agatcggcaa gcgccgtggc gagatgcgcc acatgaagct cgtgcattcc
      421 gacgccggcc ccagtgagat gcacctggaa taccacattc ctactagggg catcatgggg
      481 ctcaagaatc tcctgcttgc aaagacgcgc ggcaccatta tcatgcacca tgtcttcaaa
      541 gcctacgagc cggccgagga gcgggatctg gtcgtggcgc cgcacggatc acttgtggcg
      601 tttgaagacg ggtgtagcac cggctatgcc atctttatga cccaggagcg aggcgcgatg
      661 tttatcggcc ccggcgtgga ggtctatcgc gggatggtcg tcggcgaaaa tagccgggac
      721 gaagacctgg acgtcaacgt ctgcaaagaa aagcacctgt cgaatatgcg ggcatcgggc
      781 tccgatgaag ccttggtcct cacaccgcct cgtgaaatga cgctggagta cgcgctcgaa
      841 tatatcggac cggacgaact ggtcgagatc actccacaca atctgcgtgt tcgcaaacga
      901 ctcctcaacc cggacgatcg gcgcaaggcc cgcaaagccg gaaaataatt cttcgcgctg
      961 cgatgagaat caggaagaaa tcgccgaagc catcttctcg gcgccctcca ctctatttca
     1021 tcggctaccg aggaacagct cccgcccttg acgaactaat agattggtat gaccgcgagt
     1081 atggcgggcc gctgaaggtg cgcatagaac ctgacgcatc tgaatcatgg catgcgatgc
     1141 acggtccttg gacgtcacac gtcgtgatgc cacttcctgc cacgcacagg gacagcctga
     1201 aggagacgtt ggcctgggac cacgacctga tcggcgcggt ggcgccgtct gtcatgcctc
     1261 cgcgcgagat gccagacacg atcctgttcg ccgcacggct agctcgaggt ctcacactcc
     1321 tcgctcaagg aacagcgttc gacctgacga cccagcagta tctcaacccc tcggactgga
     1381 aggatcgtcc gctggctcat tttctcatag ccgaccacgt atccttcatg caaggtgatg
     1441 atcaactagg agatcgaagc tggtgttata cgttgggatt aagcaagttc ggaatggatg
     1501 agctggaaat ctttttccct cgcggcctgc cggatacgca tgcacgcgag ctgcttgggg
     1561 aagccgcgag cgaactcctg agatccggcc acgttcttaa ggttggcagc caattccgcg
     1621 tgacactcct gggtcagacg gtcacaatcg caaattatcg gactgcagct ccagccggac
     1681 gcacccttag ttttcgagaa atcaaagctg tctcatgatt cccttgtaca attgtgcact
     1741 tatccatgcg agtctccgct tcctaggtct ttcgggccgt tgacaaggtt tcgacccgca
     1801 cggtacaaaa cttactcaca ctgaatctca ggccgggctt aaccatgcga aggaggaatc
     1861 caaatgagtg atgttgcagc agaaatcaag gtgggcgata cagccccgga ttttaaccta
     1921 aaggatcaag accagaagga cgtgaagttg agcgactata aaggcaagaa gaatgtcgtc
     1981 ctctgctttt acccgctcga ctggagcccc gtctgtcagg gtgaaaacaa gtgcctgacc
     2041 gatgatttcc cgaaattcca gggtgctaac gccgagctgt tcggcatcag ctgtgacagc
     2101 ttcttttccc ataaagcgtg ggccgattca ttggatctca aacaccgtct gctgtccgat
     2161 gtgcatcgga ccacggcaaa ggcttatggt ctctatttcg agccgttgaa ctgctccaag
     2221 cgtgcgaccg tcatcgtcga taagaacggc aaggtcgcct atgtaaaagt tcaggagatc
     2281 aaggtcgcgc gggaagacaa ggacatcctc gccgctctgg caaagctcag ctaacttcaa
     2341 aagtctaccg cctgagtgct gagggtagga cagctccttc cctcagcact catcgtttag
     2401 cgaggcagga cctaaaatca tgtcaggaat tgtcgaagac gttagcgata cgaattataa
     2461 agagttcacc gatgcaccgg catcgattgt cgcttatggg ttggcgacct gcgagccctg
     2521 taagacatac gatcccattc tcgaagagat ggcagcaaag tttccatcaa ttaaagtcgg
     2581 taaagccaag atgcatgtgc ctggtcgctg ccgggagatc aagaaaacac acacattcga
     2641 gacctatccc accacacact tcttttcgaa gggcaaactg ctcctcgccc gtgaaggcgt
     2701 tgtagagcca actgaactcg ccgcgctgat tgcagatcat ctccagccat agtctgcttg
     2761 gacagaggag cagggcaacc tcgttctggg aatgcttgat ctccttgact ccccagaggc
     2821 ctgtagtaat gtcaaatagg ctctggtgta tacggaatac ggtcgcccgc accatgaccc
     2881 ttagcgaggc acgaatggcc tctcattcac gatcgagaaa cttccccagc tacgatgccc
     2941 aagttctgct caactcccag ccagtcatcg tttccgtcat cgacccacaa acccatcaag
     3001 ttcagttcca aaatgagact ggcctgaaga aattcggcga catcagcgga ttttcttgct
     3061 acgagaaaat tgccggctgc cagacccctt gttccttctg ccggatgccg gaggctctca
     3121 cgaccgactc ggtcgtttcc aacgaggtcg ctctgcctgg tgacaagtat ctgttggttc
     3181 attggtctaa agccccgacg atagacggac gtcttcatgt catcgaaacg atcgccgata
     3241 taacggaaca caaacggacc gaacaggcgc tacaccactc acagaaaatg gaagccgtcg
     3301 gacggttagc cgggggcatt gcgcatgact tcaacaatct gatgatggtc gtgatcggcc
     3361 atgcacagcg tctattgcag cagttctcct ctcatccatc taaaggcgag ctagaaacga
     3421 tcagtcaggc cggcatgaag gccgcagccc tgaccaagaa actcctgaca ttcggccaac
     3481 ggcaggtatt tgaaccgaaa gaagtaaaca tcaataccgt aatccgtgag atggaagata
     3541 tcctaaaacg cttgatcggt gagcacattc aaaccatgat tgtactccac ccccgcactg
     3601 ggcacgccct cgtcgatcct attcaactcg aacaagtcat catgaacctg gttttgaacg
     3661 cgcgcgatgc catgccggac ggcggccttc tcaatgtaga aaccgacaac gtggaattgg
     3721 acgaacagta cgctcgcctg caccccggct cctcagccgg tccctatgta aaaatcatgg
     3781 tgcaagacgc tgggtgtggt atggacgctg aaacccttac acatatattc gaaccgttct
     3841 ttaccacgaa agaaccgggt aaagggaccg gccttggact agccacggtc tacgcaattg
     3901 tcaaacaaag tcgagggtac atcgaggtca ccagtgaacc gggtaggggt tcacggttta
     3961 ccgtatacct acctcgcgtg ggagagccag ccatcgaggc caaagttcca catccagaca
     4021 cggaggtttg cctcatacat gaaacgatat tggtggtgga agacgatgaa ggcattcgga
     4081 agctgattac gaccatcctg caagaccagg gttacaacat cctggccgca ggagatggta
     4141 ttgaagcgct tcaaaaacta cagctctgca agggcccctg ccacctggtc atcgccgatg
     4201 tcatcatgcc ccggatgaag ggcactgcct ttatcgaggc cgtgcaggcg atgcggccgg
     4261 acgcgcgggt gctctatatg tccggctatg ctggagacac tctccaggcc aacggtgtaa
     4321 ccgccaattc gccgttcctc cagaaaccgt tcctgccagc caccctcatt gagaaggtta
     4381 gagaactcct acaaactccc gcaccgtgct aatcaacggt tgcctacgag cagaggaata
     4441 gctcacagtc cgtatgccta acccagagtg acaccactta gtcgggtctg agatctgctg
     4501 gggatcgccc ctaggcctac cagcttaact gaaaagagag cgcttctccg ataattttct
     4561 gatagatact cgcaactagg tgttagccta ttgacgtcga aggtctcact caaccagctg
     4621 attattcagg actcatcaaa agtgccattt tgtacagtcc aattcttgtc gactgacgac
     4681 acaaaatgta gttaaagcac cgccccagcc tctaaaatcg tcccgtaagc acctgaaata
     4741 attggaccgc tactgcctgc gaccggcacg gagcttgcgg tttcagggca gtggtcagac
     4801 aagagtcgag gggacactcc tcattcaggt tgtgtctcgt cggcatctca attcctgacc
     4861 agtaaggagc ttctcgaatg atgagcacta ttccacatca caacgagagg tgggtcatga
     4921 acgctacata caaggtgttt cttgcaagtc tcttggtgtg cagcctcacg gctgtatcaa
     4981 gccttgctct ggcagcgggg agcgggtcga accaagatcg ggatggacag caccgcttgt
     5041 taagtgcaga actgagggcc aagctcgaac gcgtcgagga caagctaaaa gatcgccgcg
     5101 agcaccacca taaccccctt gaagcgctgc aagctgacgt cgctagcttg aagagccaac
     5161 tggcggggct cgtaagcagc aatgcatctc tgtcgtcgca gctcaacgcg gcgaacagcc
     5221 agatcgccct ccttacctca cagatcagtg ctcttcaaac aagtggaggg agcgggggca
     5281 ctactaatcc cgcactgact gcgttggcca agtacgtaac ggttgatccg aatccgatca
     5341 acggagtaaa gggaccccac gtgatctttt caggggtgaa cctccacgtc agaagtggat
     5401 ccggctcgac tggagataat ggaacccccc
//
LOCUS       DATDNW010000090         5786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_692369, whole genome shotgun sequence.
ACCESSION   DATDNW010000090 DATDNW010000000
VERSION     DATDNW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692369"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..4151
                     /locus_tag="VI359_02335"
     CDS             21..4151
                     /locus_tag="VI359_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973122.1"
                     /translation="MVVSATGAAATTTNPDSIVDEKKSVDFEWYISPSTQEGVRQFHT
                     YSQDRELTVMGLFGAFVVEPRGSSYLEPLGAGDATAANSGWQVMIKNGTGPDFREFVL
                     IYHEVGDEAFRPVNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGINNMHVQHEYFGF
                     EDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPHGGTIRWPRSPRA
                     IDDMNLWATSGNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGSGLCQQLAGDFLF
                     HCHVAHHYVAGMWGYWRVYNTLQVGDMRNDVMPDLRELPDRKGRIKAPVSSDKLIGQT
                     VDWFGKQFTIVEKGKSNWSTNPATITIKDWVAMQLPTQGKPGHKDDEKGQIVSYDATV
                     LDWTWEGNRAMSEKESTTDNPKYKSTHPGKRHPIMFESMTGKVAWPHLTPHFGKRVMF
                     SPNHVGAPWLEMIRRDANGDESFDQAKPGENGVWSLCPENAGRKDYNVHFIKLPIKIA
                     KAQGKEPPVIDPNGLIYVLHEEEAAIRANDDLKYPLVVRGNIYDCVDWTLTSEWDDDD
                     YTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKKNKKGLPLPMNTVL
                     TAPGKKGATSITVKNAGQYHVGTLILVGADNVKGNEVVRIKDIKGNTITLSKGLKNDH
                     PANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFIAEPVGSTYHDPKT
                     GKLIRSGPIADIRTVEPVGHGVNNSFRELMVQVHDTVPHTVNIVTAGNPPGQPVEVAL
                     EAGKTVSFMMPEKIYMTPMPFLNGGTHTTGSGLNFRAGPIAQRLATNPDSSQIFNSQI
                     HGDPYTPLLRAYTGDTMVFRLLHTLMNETMTWTIAGHTFLSERYAGDANRKNSMHIGI
                     AERYDLVVPQAGGPRLQAGDYIHFNGRSSKFSEGAWGIIRVYDKEQADLKRLPAGFSV
                     KGEIPKALPVCPADAPVKTFNVVALDYPSMKFNAKAPESIEVDFERKILMTNPEAKIY
                     ALEEDTAKVASGAHPMPLTLRVNVGDCVKVNLKNKMKASKASFSAIGLAFDPKDSMGA
                     NVGNNSGDQTIAPGAERTYTYYADPFIGETTSLVWDWGNVMTNPRNGLFGAVVVGPKG
                     SKYRDPKTGADLSNKNAWAADVIIDRSVPGNETRPNYRDVALFFQDEDNIIGTSFMPY
                     VQNVAGLTGVNYRSEPYKYREEQGCSLGKVFQPCKADKPEDPATPVIESHAGDPVRIH
                     VIGASNEQNGMFSIEKHEWPIEPFMRGADQISVVEFSGSETLDAFIPSAGGPYRLSGD
                     YVYSNQRLPYSQSGQWGYLRVLPAGDQRLLPLAGSGAGMKSAAIEQPTQVIPVAAK"
     gene            4294..5223
                     /locus_tag="VI359_02340"
     CDS             4294..5223
                     /locus_tag="VI359_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY6973123.1"
                     /translation="MTTLLLTMAGWIVMTGATAWAYEEIQVSDGGTLTGTVKLEGLVP
                     KPKGYNLTTLPDPFYCGRISDGQGWRILQPFNAGPAGEFRNVVVYLEGIEKGKPFEAG
                     SLPKIEARDCQFLPFTTVVRDDQSVTVVNMDPVMHDIQAYETSHLGPRVLFNVPLPMN
                     PQHPRNFKDRSDAALYHKHMAGAPMKELVKLTKGRRVFVMQCGFHAYMESWGVAVTNP
                     YFAKTDEQGRFTLTDVPPGNYKLVVWHPYIRSAIEQPVTIGPKGTVETHIAVPAPTGR
                     LYANEVLEHDYVRFSVTEEQKKEIEPMVHKQEH"
     gene            5248..>5786
                     /locus_tag="VI359_02345"
     CDS             5248..>5786
                     /locus_tag="VI359_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY6973124.1"
                     /translation="MNGRRIAFGLGTGILPLLLLAIVPAHSYDVIEIEHGGMVEGRVT
                     LDGPVPEPKGFNLITFPDPAYCGRISNGQGWRLLHDFVVSPQGGLKDAIVLLEGVEAG
                     KPFEVSVPLIEARDCMFQPFMTIVRNGHAVEVINMDPVMHDIQGYETSIEAGARVLFN
                     TPLVMNFHHRRGDIRAIHN"
BASE COUNT         1274 a         1599 c         1800 g         1113 t
ORIGIN      
        1 tacatatcca cggctccagc atggtagtca gcgccaccgg cgcagccgct accacgacca
       61 atcccgattc gatcgtcgac gagaagaaga gtgtcgattt cgagtggtac atcagtccga
      121 gcacacaaga aggagttcgc caattccata cctatagcca ggaccgcgaa ctgaccgtga
      181 tgggactgtt cggggccttc gtggtggagc ctcggggttc cagctatctc gaaccgctcg
      241 gcgccggtga tgcgacagcc gccaacagtg gctggcaagt catgatcaag aacggcacgg
      301 gtccggactt ccgcgagttc gtgttgatct atcacgaagt cggcgatgaa gcgttccgtc
      361 cggtcaataa gaagggtgac ttcctaccgc agcgcgaccc gctgaccgat gcgtatcgtc
      421 cgggtggccg cgcgatcaac tatcgtagtg agccgttcgg catcaacaac atgcatgtgc
      481 agcacgaata cttcggcttt gaagacgagt caatgggcta cagttcctat accttcggcg
      541 acccgtcccc gactattcca cgttcctata tgggcgaccc cgcgaagttc cggctcgttc
      601 atggtgggtc ggaagtcttt cactcgcacc atccgcacgg cggaaccatc cgatggccgc
      661 gcagcccgcg ggcgatcgac gatatgaatc tgtgggccac cagcggtaat gggccggtga
      721 agtatccggt gattcgtgcc aagacagacc gagtcgacgt cgaagtcatc ggaccgtccg
      781 aagcgctgga tctcgaaacc gaatgcggtt ccggcctctg ccagcagttg gccggcgact
      841 tcctgttcca ttgccatgtg gcgcaccact atgttgcagg catgtggggc tactggcgtg
      901 tgtataacac actccaagtc ggcgacatgc ggaatgacgt gatgccggac ctgcgcgagc
      961 tgccggaccg taaaggtcgt atcaaggcgc cggtgagttc cgacaagctg atcggccaga
     1021 cggtcgattg gttcggcaag cagttcacga ttgttgaaaa gggcaagagc aactggagca
     1081 ccaacccggc cacgattacc atcaaggatt gggtggcgat gcagttgcca acgcagggaa
     1141 agcctggtca caaggacgac gagaagggcc agattgtttc ctacgatgcg acagtgctgg
     1201 actggacgtg ggaaggcaat cgtgcgatga gcgagaaaga gagcacgaca gacaatccca
     1261 agtacaagtc gacccatccg ggcaagcggc atcccatcat gttcgaatcg atgaccggga
     1321 aggtcgcgtg gccccacctc acgccacact tcggcaagcg cgtgatgttc tcgccgaacc
     1381 acgtgggcgc gccctggttg gagatgatcc gcagggatgc caacggcgac gagagcttcg
     1441 atcaggcgaa gccgggtgaa aacggcgtgt ggagtctctg tccggagaac gccgggcgga
     1501 aggactacaa cgtccatttc atcaagcttc cgatcaagat tgcgaaggcc caagggaagg
     1561 agccgccggt gatcgatccg aacgggttga tctacgtgct tcatgaggaa gaggcggcca
     1621 ttcgtgccaa cgacgacctg aagtacccgt tggttgtgcg cggtaacatc tatgactgcg
     1681 tggattggac gctgaccagc gagtgggatg acgacgacta cacgaatttc cagtcgtcga
     1741 agatcaacac acactggcat ttcttgcagt ttgataacca ggcgtcggac ggcgtgatca
     1801 ctggcttctc ctacgagcag tcggtccggc cgttcacgat gctggagaaa aagaataaga
     1861 agggcttgcc ccttcctatg aacacggtgc tcaccgcacc ggggaagaag ggtgccacct
     1921 cgattaccgt caagaatgca ggccaatatc atgtcggcac gctgattctg gtcggggcag
     1981 ataacgtgaa ggggaatgag gttgtccgta tcaaggacat caagggcaat accatcaccc
     2041 tgtccaaagg attgaagaac gaccatccag cgaacgacat cgtgacggtc gaattcgtcc
     2101 ggcagcggtt ctgggtcgat gcggacgtgg gaacggtgtt ctggcacgac catgcgttcg
     2161 gcgcgacgac gtggccgcac ggcggattcg gcacattcat cgccgagccg gtcgggtcca
     2221 cctatcatga tccgaagacc ggaaagctca ttcggagcgg ccccattgcg gatatccgca
     2281 cggttgaacc ggtgggtcat ggcgtgaaca acagcttccg tgagttgatg gtgcaggtgc
     2341 atgacaccgt gccgcacacc gtcaacatcg tgacggcggg caatccaccc ggccaaccgg
     2401 tcgaagtggc gcttgaagca ggcaagaccg tgtcgttcat gatgccagaa aagatttaca
     2461 tgacgccgat gccgttcctg aacgggggga cgcacaccac cggcagcggc ttgaacttca
     2521 gggcgggccc aatcgcgcaa cgattggcga ccaatcctga ttcatcgcag attttcaaca
     2581 gccaaatcca cggcgatccg tatacgccgt tgctgcgggc ttatacgggg gacacgatgg
     2641 tgttccgtct cctgcacacg ctcatgaacg agacgatgac ctggaccatc gcaggtcata
     2701 ccttcttgag cgaacggtac gcgggggatg cgaaccgcaa gaattcgatg cacatcggga
     2761 tcgccgaacg gtacgacttg gttgtgccgc aagccggtgg accgcggttg caggcgggcg
     2821 actacatcca ctttaatggt cgttcgtcga agttttcaga gggtgcgtgg gggattatcc
     2881 gggtgtatga caaagagcag gctgacttga agagactgcc ggccggcttc tccgtcaagg
     2941 gcgagattcc gaaggcgttg ccggtgtgtc cggccgatgc cccggtgaag acattcaatg
     3001 tggtggcgtt ggactatccg tcgatgaagt tcaacgcgaa ggcgcctgag tccatcgaag
     3061 tggacttcga acggaagatt ctcatgacta atcccgaagc gaagatttat gccttggagg
     3121 aagataccgc caaggttgcg agcggggcac atccgatgcc gctcacgctt cgcgtgaacg
     3181 tgggggattg cgtgaaggtg aatctcaaga acaagatgaa ggcaagcaag gcctccttct
     3241 cggcgatcgg cttggccttc gatccgaagg attcaatggg agcgaacgtc ggcaacaaca
     3301 gtggcgatca gacgatcgcg ccgggggccg agcgcaccta tacctactac gcggatccgt
     3361 tcatcggaga gaccacgtcg ctggtgtggg actggggcaa tgtgatgacc aatccgcgca
     3421 acggattgtt cggtgccgta gtggtgggcc ccaagggatc caaataccgc gatccgaaga
     3481 ccggggctga tctctcgaac aagaatgcct gggctgccga cgtgatcatc gatcggtcgg
     3541 ttccggggaa cgagacgcgg ccgaactatc gcgacgtggc gttgttcttc caggatgaag
     3601 acaacatcat cggcacgagt ttcatgccct atgtgcagaa cgttgcgggt ctgaccggcg
     3661 tgaactaccg gtcggaaccc tacaagtatc gtgaggagca aggctgctcg ctggggaagg
     3721 tgttccagcc ctgtaaagcc gacaagccgg aggatccagc gactccagtc atcgagtcgc
     3781 acgcaggtga tccggtccgt attcacgtga tcggggcgag taacgagcag aacgggatgt
     3841 tcagcattga aaagcacgag tggccgatcg agcccttcat gcggggtgcc gaccagatca
     3901 gcgtcgtaga gttctccgga tcagaaacgt tggatgcctt cattccgtcg gccgggggac
     3961 cctatcggct gtctggtgac tatgtgtata gcaaccagcg gttgccgtac tcgcagtccg
     4021 ggcagtgggg ctacctccga gtgttgcctg ccggcgacca gcggttattg cctctcgctg
     4081 gcagtggtgc gggaatgaag agtgcggcta ttgaacagcc gacgcaggtc attccggtcg
     4141 cagcgaagta agggccggcg cgccgcccgg tacggcgcgt gacgaagaag tgagggggga
     4201 tgtcgagagg cgtcccccct ttttcttgga cccgtctttt tggtacgatg gcgcaatttt
     4261 tctgatatga tgggattctt gacggagggg cgcatgacaa ctctgttgct gacaatggcc
     4321 gggtggattg tgatgaccgg agccacggcc tgggcctacg aagaaatcca agtgtccgac
     4381 ggcgggactc tcactggcac cgtgaagctg gaggggctag ttccgaagcc gaaaggctac
     4441 aacctcacga cgttgccgga tcccttctat tgcgggcgta tttccgacgg gcagggctgg
     4501 cgcatcctac agcccttcaa cgcggggccg gccggcgagt ttcgcaacgt cgtggtctat
     4561 ctggagggga ttgagaaggg caaacctttt gaggcgggca gccttccgaa gatcgaagcc
     4621 cgcgattgcc aatttctgcc gttcacaacc gtcgtgcgcg acgatcaatc ggtgacggtg
     4681 gtcaacatgg accccgtcat gcatgacatt caggcctatg aaacctcgca tctcggtccg
     4741 cgcgtgctgt tcaacgtgcc gctgccgatg aacccgcagc acccccgcaa cttcaaggat
     4801 cgcagcgacg ccgcactgta ccacaaacac atggctggcg cgccgatgaa ggaactggtg
     4861 aagctcacca agggccgccg ggtcttcgtg atgcagtgcg gcttccatgc ctatatggaa
     4921 agttggggtg tggccgtcac caatccatac tttgccaaga cggacgagca gggccggttc
     4981 accctgaccg atgtgccgcc tggtaactac aagctggtgg tctggcatcc ctacatccgg
     5041 agtgcgatcg agcaacccgt caccatcggt ccgaagggaa cggtggagac ccatatcgcg
     5101 gtcccggccc cgaccggacg gctctatgcc aacgaggtgc tggagcatga ttacgtgcgt
     5161 ttcagcgtga cggaagagca gaaaaaggaa atcgaaccaa tggtccacaa gcaggagcac
     5221 tgagcaaact cctcagagga gcaaggggtg aatggaagac gcatcgcatt cggtttggga
     5281 acgggcatcc tgccactgct gttgttggcg attgtgcctg ctcactcata cgacgtcatt
     5341 gagatcgagc atggggggat ggtcgaaggc cgagtgacgc tcgacggccc cgtgcccgag
     5401 cccaaagggt tcaacctcat cacgtttccc gacccggcct actgtgggcg catttcaaat
     5461 ggacaaggtt ggcgcctcct ccatgacttt gtggtcagtc cgcagggcgg actgaaagac
     5521 gccattgtcc tcctggaagg agtcgaagcg ggcaagccgt ttgaagtgtc cgtcccctta
     5581 atcgaggccc gcgactgcat gtttcaaccg ttcatgacca ttgttcgcaa cggccatgca
     5641 gttgaagtga tcaacatgga cccggtgatg cacgatatcc aggggtacga gacttccatc
     5701 gaagcaggcg cgcgggtgtt gttcaatacg ccgcttgtca tgaattttca ccaccggcgg
     5761 ggcgacatcc gcgcgataca caacca
//
LOCUS       DATDNW010000091        13706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_350553, whole genome shotgun sequence.
ACCESSION   DATDNW010000091 DATDNW010000000
VERSION     DATDNW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350553"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="kdpC"
                     /locus_tag="VI359_02350"
     CDS             <1..430
                     /gene="kdpC"
                     /locus_tag="VI359_02350"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEY6973125.1"
                     /translation="VIGSKLIGQYFDKPEYFWGRPSATAPFPYNAAASGGSNLGPTNP
                     ALIEAVKARVAALRAADPGNELPIPVDLVTASGSGLDPHISPAAALYQLKRVSRARGL
                     DDDSVLRLVTQHTEGRQFGFLGESRVNVLELNLALNALKP"
     gene            complement(639..920)
                     /locus_tag="VI359_02355"
     CDS             complement(639..920)
                     /locus_tag="VI359_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973126.1"
                     /translation="MENIDLGTLITGAVCGVGIIELIIWIVVVIRNPGYDFLRPEIVP
                     NVTSNALENPNASRAVVSQPPFRRLRIPRVPLSSNQSSHQDEHAYDKQK"
     gene            1125..3830
                     /locus_tag="VI359_02360"
     CDS             1125..3830
                     /locus_tag="VI359_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY6973127.1"
                     /translation="MTNQKRPDPDALLKRVQAEEARQLQGKLKIFFGANPGVGKTYAM
                     LEAAHEQRRDGVDVVVGVVETHGRAETEALLNGLEVVPRRAVDYRGTVLQEFDLDAAL
                     ARRPTIILMDELAHTNAPGMRHAKRWQDVQELLKAGITVYTTVNVQHLESLNDVVAQI
                     TGVRVRETVPDSVLERADDVELIDLPPDDLLQRLRDGKVYVPEQIQHAIQHFFAKGNL
                     IALRELALRRTAERVDQQMEVYRRDHAVVRTWPAGETIMVCVNMKPRGPRLIRAARQM
                     AASLHAKWIAAYVQIPRHLRQAQSERDRLVQTLRLAEQLGAEVVTLSGDNVTQELLSY
                     ARSRNVTKIIVGKPLRSWWKEWIFGSVVSDLVHQSGEMDIYVITGEAAEGQPLVRRSL
                     RRTSDLSGYGYASLGVLSATAVAWLMFPYFGAANLIMMYLIAVITVAIRWGRGPSVLA
                     SVLSVAAFDFFFVPPYFSFAVSDIQYLLTFGVMLVVALVISNLAVRIREQAELARYRE
                     KRMRVLYSMSRDLATHRGTGLLSHLAAKHLREVFDSQVAIFLADADKRLQLQRGEELY
                     FEFDPKEAGVAQWVFEHNERAGLGTDTLPGASALYLPLVGSAGPIGVVAVRPTESSRL
                     LEPDQIHLLESLVNQVALAIERTRLSEEAERAHVRVETERMRNAILSSVSHDLRTPLA
                     TITGAASSLVEEQGKMDPAARRDLSQSIYREADRLDRLLKNLLDMMRIEAGAVQLSKE
                     WHPVDEVVGAALARLEGRLHDHTVNAAFPADLPLVLVDGVLLEQVVINLVENAVKYAP
                     QGSAIDMSASASDREVVVEVADRGPGIPVGEETRIFDKFYRGKFAREGGVGLGLTICR
                     GIVEAHGGRIWAENRRGGGAVFHFTIPLMESQPSVEAEQAEPKPA"
     gene            3894..4571
                     /locus_tag="VI359_02365"
     CDS             3894..4571
                     /locus_tag="VI359_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973128.1"
                     /translation="MLVIEDEPEIRRFLRTTLPAHGFRLYEAATGRDGLTEAKARNPD
                     LILIDLGLPDLDGSEVIRQVREWTATPIIVLSARDQEQMKVAALDLGADDYVTKPFGV
                     NELLARMRTALRHVARVAEAGGESVFTLGGLRVDLGRRQVFVSDKEVHLTPIEYKLLT
                     TLVRHAGKVLTHRQLLKEVWGPLHVDEGHYLRVYMRQLRNKVEANPAHPRYLVTELGV
                     GYRLRTE"
     gene            4710..5984
                     /locus_tag="VI359_02370"
     CDS             4710..5984
                     /locus_tag="VI359_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6973129.1"
                     /translation="MQRVNAPIHDSARAEILPSKPARRWLNRLNWLAVGWTLVALVSV
                     SEAQDVNQGSEENAVPPRASPTTSESSTSDWHYGAYLDLSYIVNFNFPENRRWRSRTT
                     TERNNELTPNMGLVYVRKDATESSRWGTELGFQGGYDADNFAFLVNEPKVDGANTLRY
                     IHRANVSYLAPVGNGLTVTAGLFNSLIGYESLYAKDNFNYTRSWAADNTPYMMFGVNA
                     KYPVNEDVTVTAFIINGYYHLSNPNDQPSYGGQVAWKPAQRVTVTNTLYYGPDQQSTS
                     LEFWRLFSDNIVEWKGDDVTVALTYDIGTERILNQPGNPRTFAMAGVLEGRWHIAGPW
                     GVALRPEFYWDRNGRWTGSEQFIKAVTTTAEYKFPYWWTNTVVRLEYRYDESTGTQGG
                     FFTRNQVSPGVIGLTAAQHMLLVGILWTFDSP"
     gene            complement(6019..6714)
                     /locus_tag="VI359_02375"
     CDS             complement(6019..6714)
                     /locus_tag="VI359_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973130.1"
                     /translation="MTWLRVIVVLLCFLTGCASSQGLDPKPLTELLRYEEERFIGEQP
                     LTPIAARPLSPTTLKLGLYLNPTGFFKREFEWTDGDRETVLVWANGLQASRIVAGASF
                     VPQSSLKGNSLTELRESAARYGADLLLILDGAAAIDRYNNYKASLLYWTILGAYLADG
                     THSDALCVLRGAVWDAKTGTQLSTDDAQGTAKQIGPAARVEDRTAVSQACRQALTQLL
                     TAVKNRLAHPKSP"
     gene            complement(6711..7634)
                     /locus_tag="VI359_02380"
     CDS             complement(6711..7634)
                     /locus_tag="VI359_02380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6973131.1"
                     /translation="MTTQPITPRGTSIGWIGTGVMGRAMCGHLLAAGYRVTVHNRTRA
                     KAQPLIDRGAHWAESPRETAARSDVVFTMVGFPQDVRQVYFGVDGILAGARPRTFLID
                     MTTTHPSLSCEIADAATAKNLSAIDAPVSGGDVGARNATLSIMIGGEKTAVQVVVPLF
                     EILGKKLVHQGGPGSGQHAKLCNQIVIAGTMVGVCESLLYGFQAGLDLPRMLDVIRSG
                     AAACWTLENLAPRILQRNFDPGFLVDHFIKDMGIALEESRRMGLALPGLALAHQLYEK
                     VQSFGHGRSGTHALMIALEDLSHVTMSTQAT"
     gene            complement(7650..8105)
                     /locus_tag="VI359_02385"
     CDS             complement(7650..8105)
                     /locus_tag="VI359_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973132.1"
                     /translation="MTKRLVHIVVSVWLGWSVGFSSAAAGEPITIREILDEPMSFHLK
                     QVTLHGTVRNVTPLDPYKLPAGTACYGAYLFYLEDETAVINVAVYGLCGVPTVKDPDV
                     EDGQRIELISTIQAPSHGGYYLSLQGVKVATDQEGIIQAVADRITPLTD"
     gene            8198..9568
                     /locus_tag="VI359_02390"
     CDS             8198..9568
                     /locus_tag="VI359_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6973133.1"
                     /translation="MTVNSRQLDTYVTETRPRFEDLLGQMVEIPSISMDPAKAGDIRR
                     MANLAVQILINQGAEAQIVETGGYPIVSGGWTTGANHPTVTIYNHLDVQPAQEPEWKQ
                     EPFVFRKENGLYRGRGATDDKGPALAAMLGAHYVIEQGVPINVRVLWELEEEIGSPHF
                     LAGLKHHAAIPKPDSVVVSDTIWIAKGRPAMPYGLRGLLGARLTLRTGLKDAHSGVTG
                     GAARNPLAELMEVASACVDAKTGKVKIPGFYNGVVEPSKAEIKSFLRSGFQVRRFKEA
                     YGFHTLRTHDPAEITRRIWAAPTFEIHGLTGGYHGPGVKTVVPGQGELKVSMRLVPNQ
                     TPEKVFALFKKYVARMNPNVKVEREGMLHPFKGSFEGPYVEAVKRAAKAGFGKEPAFI
                     REGGSIGAVVTMQKRWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGMKAFAHYFSEL
                     AGMSKR"
     gene            complement(9581..10276)
                     /gene="aat"
                     /locus_tag="VI359_02395"
     CDS             complement(9581..10276)
                     /gene="aat"
                     /locus_tag="VI359_02395"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY6973134.1"
                     /translation="MFRLSQRGDNLRFPPAEQASPEGLLAVGGDLRVERLLEAYRHGI
                     FPWYNDDQPILWWSPDPRAVLFPGKLHISRSLKRTMRLGGFTVTLDTRFRDVMQGCAG
                     PRPQYPDGGTWITEEMMEAYVRLHELGYAHSVEAWQEGQLVGGLYGVALGGIFFGESM
                     FTRVPDASKVALVSLVRQLQAWDFRIFDCQQPSRHIKMLGAEDISRRDFLDHLAAALA
                     LPDRRGRWCFDSR"
     gene            complement(10391..12100)
                     /locus_tag="VI359_02400"
     CDS             complement(10391..12100)
                     /locus_tag="VI359_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973135.1"
                     /translation="MACRFTRIANPLVLASILTVVTLGFSGPVVAAPTKQQKPTVTKS
                     TPATDTARRYAEAIAKGDRVTVGQLDFACQYRIVAASPAGVKSYPPTNDPSYDSCWQT
                     LKAAHASILKRDDVAMAILWPSAGPLAFFGDELPRTPASAFVMDELGISPPGTGLHVQ
                     VAKSQTIPSGSFRLKPNGKIVGVPTTLVFLTVSYQDPLTSPVTYAAGVVKRANTIKRA
                     RGALKSVTTQWVVFTGLKKHGFPGDAAVFNLPVITQPETPGMMAEKIPFTTETSRALP
                     DSLVWWGPTDQPGTLTAAAARAATFPDLRDRVALLNRILIIDPNQPDALTVLTRHLYA
                     VFLREGKNGHQVIVKDPALSLVVDEFYWNVYAQSIRMDLANDMERGGSPEPTPADFLF
                     RMLPALEALAKVRPEQLDNRFRLGAAFRWNNEQQAAIDTDEALVKDIPEGRKAAKAEA
                     LLQLAWSRMHKVAWNRIMDDPEINRAYGDAEASLAIADLPIDKFLAEYTMAYCMIFMP
                     NYGDKAKLLHHLTEAKRWFDEVPGKTDEVWRYFLHSELLKAVLDADPSFKPILASAEE
                     HKS"
     gene            complement(12203..12580)
                     /locus_tag="VI359_02405"
     CDS             complement(12203..12580)
                     /locus_tag="VI359_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6973136.1"
                     /translation="MRAAFAIIIPGLLGVGLTSVAAQSDQTKWERGRIIYEQRCLDCH
                     GSEGRGDGRNALSLSPRPGNLISAATSAKSDQDLLKIIANGRPRTAMPAWKDELSDED
                     QQAVLAYIRSLVRFSRSLTPPAP"
     gene            complement(12611..13012)
                     /locus_tag="VI359_02410"
     CDS             complement(12611..13012)
                     /locus_tag="VI359_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY6973137.1"
                     /translation="MWRVSSRTVLFLLYLSWPVPPITAAEPLVVAVDIQSRVFIPHRA
                     ALHHGQPTVLVFRNHDSELHAFVPSELFAGVNLNVTGNGAPEFGPEGFKRAIIPPEGS
                     VEIHFTPERAGEYSYICDMPGHQMAAMIVVE"
     gene            complement(13042..13350)
                     /locus_tag="VI359_02415"
     CDS             complement(13042..13350)
                     /locus_tag="VI359_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY6973138.1"
                     /translation="MTKKAISRKKAGVQGGSGHKHETGRCLHILQRLSAYIDDELASN
                     VCDEIRRHLGACPNCEVFVESLRHTVALCRHRPTPALSPTDRQALRENILRAARAQAR
                     "
     gene            complement(13347..>13706)
                     /locus_tag="VI359_02420"
     CDS             complement(13347..>13706)
                     /locus_tag="VI359_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6973139.1"
                     /translation="RELSLDEFVPTSEGEFRLQIPVDGLTPEEAFQNKELRQALDQAI
                     EKLPRKYRMVLVLRDMEGLSAKEVGTVMGLNERAVKSRLHRARLFVRRELSARGITEG
                     PSEDARATTQTSGGYDR"
BASE COUNT         2850 a         3885 c         4158 g         2813 t
ORIGIN      
        1 agtgatcggg tccaagttga tcggacagta ttttgataaa ccagaatatt tttggggccg
       61 tccgtctgcg accgcgccgt ttccctataa cgccgccgcg tcgggtggat cgaacctcgg
      121 gcccaccaac ccggcgctca tcgaggcggt caaagcgcga gtggccgcct tacgagccgc
      181 ggatccgggc aacgagttgc ccatacctgt cgacctggtc accgcctccg gaagcgggct
      241 cgatccccac atcagcccgg ctgctgccct gtatcaactg aagcgagtct cccgcgctcg
      301 aggcctggat gatgatagcg tgctgaggct ggttactcag cacacggaag ggcgccagtt
      361 cgggtttctt ggagagtcgc gcgtcaacgt tcttgagttg aatcttgcgc tcaatgccct
      421 gaaaccctga gctcgtccag accgaaatat ctctgagagg cataaggttg ccagaacaaa
      481 agcacaaaac aggattatct gacgaggatg tcgtcttgat ttcgcagcgt ttatgtctcc
      541 gatggaaggt gctgcaaggc gttggcgcga cactgatcac gctctccatc tttatcgaat
      601 ggccggccct tgccgacgcg agtcttccgc agacctcatc atttctgctt gtcgtaggcg
      661 tgctcgtctt gatggctgga ttgattggag gaaagtggga cgcgggggat ccgtaagcga
      721 cgaaatggtg gctgggacac cacagcccga gaagcattcg ggttttccag tgcgttggaa
      781 gtcacattgg gaacgatctc gggtcgaagg aaatcgtacc caggatttct gatgaccacc
      841 acaatccaga tgatgagctc tataatccct acgccacaaa ccgctcccgt aatcagcgtc
      901 cccagatcga tgttttccat aatggcacct cctttatatc ccgaagcaca gaataacctt
      961 agagacatca aaatgtggta agaagacacc ggcaggctgt aaagcccgcg taaaaatttg
     1021 cctggcctag agctaccaga attgacggat ttccaggcct tttctaggat ttgttgtgcc
     1081 atacgaattg ctcgaacacc cggtgtggcc cggaacttag acctttgacg aaccagaaac
     1141 gaccagatcc ggacgcgctg ctcaagcgtg tgcaggccga ggaagcccgg cagctccagg
     1201 gcaaactcaa gatattcttt ggcgccaacc ccggcgtggg gaaaacctac gccatgctgg
     1261 aagcggcgca cgagcagcgc cgggacgggg tggacgtcgt cgtcggagtc gtggagacac
     1321 atggtcgggc ggagaccgag gccctgctga acggacttga ggtcgtcccg cgccgcgccg
     1381 tcgactatcg cggcaccgtc cttcaggaat tcgatcttga cgcagccctg gcgcgtcgcc
     1441 ccaccatcat cctcatggat gaactggcgc acaccaatgc gcctggcatg cgtcacgcga
     1501 agcggtggca ggatgtccag gagttgttga aggccggtat cacggtctac accacggtca
     1561 acgtgcagca cctggaaagc ttgaatgacg tggtggctca gatcaccggc gtgcgagtgc
     1621 gcgagacggt tccggattct gtgctcgaac gggcggatga cgtggagctc atcgaccttc
     1681 cgcctgacga ccttctgcaa cggctcaggg acgggaaagt ctacgtgcca gagcagatcc
     1741 agcacgccat ccagcatttc ttcgccaaag gcaatctgat cgcgcttcgt gaactcgcgt
     1801 tgcgccgcac ggctgagcgc gtcgatcagc agatggaagt ctaccggcga gatcacgcgg
     1861 tggtgcgaac ctggccggct ggggagacca ttatggtgtg cgtcaacatg aagccacgcg
     1921 ggccgcgcct gatcagggcg gcgcgccaga tggccgccag cctgcacgct aaatggattg
     1981 ccgcctatgt tcagattccg cggcatctcc gtcaagccca gagcgagcgg gatcgtttgg
     2041 tgcagactct tcgattggct gaacagctgg gagccgaagt ggtgacattg agcggcgaca
     2101 atgtcaccca ggaactcttg agctatgcgc ggagccggaa cgtcactaag attattgtcg
     2161 gcaagccgct gcgatcctgg tggaaagaat ggattttcgg ctccgtcgtg tcggacctcg
     2221 tccatcaaag cggggagatg gacatctatg tgatcacggg ggaggcagcc gaaggccagc
     2281 cccttgtccg tcggagtctc cggagaacca gcgatctgtc agggtacggt tacgcctcgc
     2341 tcggagtctt aagtgccacg gcggtcgcct ggctgatgtt cccttatttc ggcgcggcca
     2401 atctcatcat gatgtatctc attgcggtga tcacggtggc cattcgctgg gggcgcggtc
     2461 cgtccgtgct cgcatcggtg ctgagcgtgg cggcgttcga ctttttcttc gtcccgccct
     2521 acttctcatt cgccgtgtcc gacattcaat atttattgac cttcggtgtc atgctggttg
     2581 tggcattagt catcagcaat ctcgccgtgc gtattcgaga gcaagccgag ttggcacggt
     2641 accgggaaaa acgaatgagg gtgctgtact cgatgagccg tgatctcgcc acccatcgag
     2701 ggacgggcct gctgagtcac cttgcggcga aacaccttcg agaggtgttc gacagccagg
     2761 tcgcgatctt tctggcggat gccgataaac ggctgcaatt gcaacgcggc gaagaactct
     2821 attttgagtt cgaccccaaa gaagccggag tcgcccaatg ggttttcgag cacaacgagc
     2881 gagccgggct cggaaccgat acacttcccg gggccagcgc gctgtatctc cccttggtcg
     2941 ggtcggccgg accgatcggc gtcgtggcgg tgcggccgac cgagtccagc cgactcctgg
     3001 agcccgatca gatccatctg ctcgaatctc tcgtgaacca agtcgcattg gcgatcgaac
     3061 ggacccgcct ctcggaggag gcggagcggg ctcatgtgcg ggtggaaacg gagcggatgc
     3121 gcaacgccat tctcagttcg gtctcacacg acctgcgtac tccgctggcg accatcactg
     3181 gcgctgccag tagcttggtg gaagaacagg ggaaaatgga ccctgctgcg cgacgcgatc
     3241 tctctcaatc tatttatcga gaggctgatc gcctggatcg cttgctcaag aaccttcttg
     3301 acatgatgcg cattgaggcg ggggccgtgc aactcagcaa agagtggcac cccgtcgatg
     3361 aggtcgttgg tgcggccctg gccagactgg agggacggtt gcacgatcat accgtgaacg
     3421 cagcgtttcc tgccgacctg ccgctggtgc ttgtcgatgg tgtacttctg gaacaggtgg
     3481 tgatcaacct tgttgagaac gccgtgaagt acgcacccca gggaagcgcg atcgatatgt
     3541 ctgcctcggc gagcgaccgt gaagtggtcg tcgaggtcgc agatcgaggc ccagggatcc
     3601 cggtcggtga ggagacccgc atcttcgaca aattttatcg cggcaagttt gcgcgagagg
     3661 ggggggtggg gctggggttg acgatttgtc gcggcatcgt ggaagcgcat ggtggtcgta
     3721 tttgggcgga gaaccggcgg ggagggggtg cggtgttcca ttttacgatc cccttgatgg
     3781 agagtcagcc gtctgtcgaa gcggaacagg ccgaaccgaa gccggcctga tcgactccgg
     3841 aaaagagttg tggtcgggag gccgtcaccg ttatgtccgg acaggaagtt acagtgcttg
     3901 tcatagaaga tgagccggag attcgccggt tcctgcggac gacgctgccc gcgcatggct
     3961 ttcggttgta cgaggctgcg acaggacgag acggcctgac cgaggccaaa gcgaggaatc
     4021 ccgatctcat cttgatcgac ctcggtctgc cggatctcga cggcagcgaa gtgattcgcc
     4081 aggtgcggga atggaccgcc acgccgatca ttgtgctgtc ggcccgcgac caggaacaga
     4141 tgaaagtggc ggcgcttgac ctgggcgcag acgactatgt gaccaagcct ttcggagtca
     4201 atgaactcct tgcacgaatg cgcaccgccc tcaggcacgt cgcccgagtg gctgaggcag
     4261 ggggcgaatc tgtgtttaca cttggtggat tgcgggttga tcttggccgg aggcaggtct
     4321 tcgtgtcgga caaagaggtt cacctcacgc cgatcgaata caaattactc accacactcg
     4381 tccgccatgc cggaaaggtg ctcactcatc ggcaattgct gaaagaagtc tgggggccgc
     4441 tgcacgtaga cgaggggcat tatcttcgag tgtacatgcg gcagctccga aacaaagtcg
     4501 aggccaatcc agcccatcca cgttatttag tcactgagct gggagttggc tatagacttc
     4561 ggacggaata agctcacgct ccctcctctt acaattcctt tcccgctatc cctaaccggt
     4621 cggttttgat cccgttttgg cgtgcaacag ggtatagttc gccgggtcaa gctcaagaat
     4681 gctttctgag taatcagaag gagtgtacgg tgcaacgggt caatgcgccc atacacgaca
     4741 gcgcccgcgc tgaaattctg ccgtcgaagc ctgcccgtcg ctggttgaat cgactcaact
     4801 ggctagcggt cggatggacg ctggtcgcac tggtctctgt cagtgaggct caagacgtga
     4861 accagggatc tgaggagaac gccgtgcctc cccgtgcttc ccccactacc agcgaatcat
     4921 cgacaagcga ctggcactac ggcgcctatc tcgacctcag ctacatcgtg aacttcaatt
     4981 ttcctgagaa ccgccggtgg cgcagccgca ccaccacaga acgcaacaat gaactgactc
     5041 ccaacatggg tctagtctat gtcaggaaag atgcgacgga atcctctcgt tgggggacgg
     5101 aactggggtt ccagggcggg tatgacgcgg ataacttcgc atttctcgtc aatgagccta
     5161 aggtggacgg tgcgaacact cttcgctaca tccatcgcgc caacgtatca tacctggcgc
     5221 cggtcggaaa cggcctgact gtcaccgcgg gactcttcaa tagcctgatc gggtacgagt
     5281 cgctctacgc caaggacaat ttcaattaca cccgctcctg ggctgcggac aacacgccgt
     5341 acatgatgtt tggcgtgaac gccaagtatc cagtgaatga ggacgtgacc gtcacggcgt
     5401 ttatcatcaa cggatactat cacttgtcca atccgaacga tcagcccagc tacggtgggc
     5461 aggttgcgtg gaaaccggct cagcgggtta ccgtgacgaa cacgctctac tacgggccgg
     5521 accagcagag cacctcgctc gagttctggc gcctcttcag cgacaacatt gtggaatgga
     5581 aaggggacga tgtgacggta gccctgacct atgacatcgg aacggaacgg atcctcaatc
     5641 agccaggtaa cccgcgcacc tttgccatgg ccggtgtctt ggagggaagg tggcacatcg
     5701 ccggtccctg gggcgtcgcg cttcgtccgg aattctattg ggaccggaac ggacggtgga
     5761 ctgggtcgga gcagttcatc aaggcggtca ccaccaccgc cgagtacaag ttcccctatt
     5821 ggtggacgaa cacggtggtc cggctcgaat accggtatga cgagtcgacc ggcacccaag
     5881 gagggttctt tacgcgaaac caagtgagtc ccggcgtgat cggtctcacg gctgctcaac
     5941 acatgttgtt agtggggatt ctctggacct tcgactcacc atgactgcga cgtcgaaccg
     6001 tctggaatca gagtcagcct acgggctctt aggatgtgcc agccgattct tcacggcggt
     6061 taatagctgc gtgagtgcct gccggcacgc ctgtgagact gcggtccgat cctctaccct
     6121 tgccgctggg cctatctgct tggcggtgcc ttgcgcgtcg tcggtggata gctgagtccc
     6181 cgtcttagca tcccatacag cacctcggag aacgcacaga gcatcgctgt gagtcccgtc
     6241 cgcgagatac gctcccagaa tcgtccaata gagaagggac gccttgtaat tattgtaccg
     6301 atcgattgcc gcggccccgt ctaggatcag cagcaagtct gcgccgtaac gagcggcgga
     6361 ttctcggagc tcggtgaggc tattgccctt gagcgaggat tggggcacga aactcgcgcc
     6421 cgccacgatt ctgcttgcct ggagaccgtt tgcccagacc agcactgtct cacgatcgcc
     6481 gtccgtccat tcaaattccc gtttgaaaaa ccccgtcggg ttcaagtaga gcccgagctt
     6541 gagagtagtg ggacttaaag gacgagccgc gatgggagtc agcggctgtt ctccaataaa
     6601 gcgctcttct tcgtaacgca gcagctcggt gagcggtttc ggatccagac cttggctgct
     6661 cgcgcaaccg gttagaaagc acagaagcac gactatgacg cgcagccacg tcatgtggcc
     6721 tgtgtgctca tcgtgacgtg tgagagatct tcgagtgcga tcatcagcgc atgggtaccg
     6781 gaccggccgt ggccgaaact ctgaactttc tcgtagagct gatgcgcgag agccagaccc
     6841 ggcagcgcca gccccatccg cctcgactct tccaacgcaa tacccatatc cttgatgaaa
     6901 tgatccacca agaagccggg atcaaaattc cgctggagga ttcgtggcgc cagattctcc
     6961 agcgtccaac aggccgcagc cccgcttcgg atcacatcga gcattcgagg gagatcgagg
     7021 ccggcttgaa acccatagag cagactttcg cacacgccga ccatcgttcc agcgatgacg
     7081 atctggttac acagcttcgc atgctgaccc gaaccgggcc cgccctgatg gacaagcttc
     7141 ttcccgagta tttcgaataa aggcaccacg acttgaactg ctgtcttctc gcctccgatc
     7201 atgatggaaa gagtggcgtt gcgcgcaccg acgtcgccac cggacaccgg cgcgtcgata
     7261 gcagagagat ttttggctgt ggcggcgtca gcaatctcac agctcaatga agggtgcgtc
     7321 gtcgtcatgt cgatgaggaa cgttcgaggg cgagcgccgg ccaagatgcc gtctactccg
     7381 aaatagacct gccggacgtc ctgcgggaat ccgaccatgg taaagacaac gtctgacctt
     7441 gcggcggtct cacgcggact ctccgcccag tgcgcgcctc gatcaatcaa tggctgggcc
     7501 ttcgctcgcg tccggttatg gaccgtgacg cgataaccgg ccgcgagcaa atgtccacac
     7561 atggctctgc ccatcacacc ggtgccgatc catccgatac ttgtgccgcg aggcgtgata
     7621 ggttgtgtcg tcatcgcatg agtgcctgtc tagtcggtca gcggtgtaat tcggtcggct
     7681 acagcctgga tgattccctc ttgatctgtc gccactttta ccccttgcag gctcaagtaa
     7741 tacccgccgt ggctgggagc ttggattgtg gagatgagct caattcgttg gccgtcctct
     7801 acgtccggat cttttactgt ggggaccccg cagaggccat acacggcgac gttgatcact
     7861 gctgtctcgt cttccaggta gaacagataa gctccgtaac aggcagtgcc agccggcaac
     7921 ttatagggat cgagaggagt cacattccga acggtgccgt gcaacgtcac ctgtttgaga
     7981 tgaaacgaca tcggctcgtc caagatctcg cggatcgtga tgggttcacc ggcggcggct
     8041 gatgagaagc cgaccgacca tccaagccac acagagacca cgatgtgcac cagccgtttc
     8101 gtcatcgctc cattttacag aacgttgcag cagaagtccc ttcctcggca ctcacacagt
     8161 cgttataatc cgcggcgaca tcctgtggag ggctacgatg accgtgaatt cgcgacaact
     8221 cgatacctat gtcacagaga cacgcccaag gtttgaagac ctgctcggcc agatggtgga
     8281 aattccgtcg atcagtatgg atccagccaa ggctggggac atcaggcgaa tggccaatct
     8341 tgcggtgcag attctgatca atcagggtgc ggaggcgcag attgtcgaga ccggcggcta
     8401 tccgatcgtg tccggtggct ggacgaccgg cgcgaatcac ccgaccgtca cgatttataa
     8461 ccatctcgac gtgcagccgg cgcaggaacc agagtggaag caggagccgt ttgtctttag
     8521 aaaggaaaat ggtctctatc gcggccgcgg agcgacggac gacaaggggc cggcgttggc
     8581 cgcaatgtta ggcgcacact atgtcatcga acagggcgtc ccgatcaatg tccgtgtctt
     8641 gtgggaactc gaggaggaaa tcggcagccc gcatttttta gctgggttga agcatcatgc
     8701 ggcgatcccg aagccggact ctgtcgtcgt gtcggacacg atctggattg cgaagggccg
     8761 accggccatg ccctatggtc tgcggggatt gttgggcgcg cggttgacgt tacggaccgg
     8821 cttgaaagat gcccactcgg gcgtgacggg cggtgcggcg cgcaatccac tggcggaatt
     8881 gatggaagtc gcgagcgcct gtgtcgatgc caagaccggc aaggtgaaga ttcccggatt
     8941 ttacaatggt gtggtcgaac catcgaaagc cgagatcaag agttttctga ggtcaggttt
     9001 tcaagtaagg cgattcaaag aggcctacgg gtttcacacg ctccgcacgc acgatccggc
     9061 cgagatcacc agacggattt gggcagcgcc gaccttcgag atccatgggc tcaccggcgg
     9121 ctaccacgga cctggcgtga agaccgtcgt gccgggacag ggcgaactca aggtcagcat
     9181 gcgcctcgtg ccaaaccaaa cgccggagaa ggtgtttgca ttattcaaga aatacgtcgc
     9241 caggatgaac ccaaacgtga aagtcgagcg ggaagggatg cttcatccct tcaagggcag
     9301 ttttgaaggg ccatatgtcg aagccgtgaa gcgagcggca aaggctggat tcggcaaaga
     9361 accggcgttc attcgtgaag gaggttcgat cggcgccgtc gtgacgatgc agaagaggtg
     9421 gaaagtgccg atcctcttca tgggcctcag tctcccggag catggctatc acgcgccgaa
     9481 cgaatatttt gattggggcc aagcatcggg tgggatgaag gcgttcgcac attatttttc
     9541 ggagctggca ggaatgagca aacgataaac ggtgggaagc ttatcttgaa tcaaaacacc
     9601 atcggccgcg ccgatcaggg agggcgagcg ctgcggcaag atgatcgaga aagtcacggc
     9661 gggagatgtc ttcagcgccg agcatcttga tgtggcgcga gggttgctgg cagtcgaaga
     9721 tccgaaagtc ccaagcctgg agctggcgca cgagcgagac cagcgcgact tttgaggcgt
     9781 ccggcacgcg cgtgaacatc gattcgccga aaaagatgcc gccgagcgcc acgccataga
     9841 gcccgccaac cagctgtcct tcctgccacg cttcaacgga atgggcgtag ccgagttcat
     9901 ggagccgcac ataggcttcc atcatctctt ctgtaatcca ggttccaccg tcgggatact
     9961 gcggtcgtgg tcccgcgcat ccctgcatca cgtcccgaaa cctcgtgtcg agcgtgacgg
    10021 taaacccacc gagtcgcatc gttcgcttca ggctgcgcga aatatggagt ttgccgggaa
    10081 agagcacggc ccggggatcg ggtgaccacc acaagatcgg ctgatcgtcg ttgtaccagg
    10141 ggaaaatgcc gtgacgatag gcttcgagca gccgctctac ccgcagatcg ccgccgacgg
    10201 cgaggaggcc ctcgggcgaa gcctgttctg ccggtggaaa gcgtaggtta tcgccgcgct
    10261 ggctcagcct gaacatgctg ctacacgata ccgcaggcgg caggaaaaga ctatcacgac
    10321 tggatgagag aggtgtttac ttcgatttcc ctaggtacca ggcggagcac cacgtggtgt
    10381 tccggcgtct ctatgatttg tgttcttctg ccgacgccaa aatcggtttg aagctcgggt
    10441 cggcgtcgag gacggctttg agcagttccg agtgcagaaa atagcgccag acctcgtcgg
    10501 ttttccccgg cacttcatcg aaccagcgtt tcgcttccgt caagtgatgt agcaacttag
    10561 ccttgtcgcc gtagttcggc atgaagatca tgcagtaggc catggtgtat tcggcgagaa
    10621 acttgtcgat cggaaggtcg gcgatcgcaa gcgatgcctc ggcgtctcca taggcccgat
    10681 tgatttctgg atcgtccata atccggttcc acgccacctt gtgcatgcgc gaccaggcga
    10741 gctggagcaa ggcctcggcc ttggctgcct tacgcccctc cggaatgtcc tttaccagcg
    10801 cttcgtccgt gtcgatcgcc gcctgttgtt cgttgttcca tcgaaaggcg gcgccgagcc
    10861 taaaacggtt gtcgagttgc tccggtctga ccttggccaa tgcttccagc gccggcaaca
    10921 tgcgaaagag gaaatccgct ggcgtcggtt ccggcgaccc gcccctttcc atgtcattcg
    10981 ccagatccat gcggatcgat tgggcgtaga cgttccagta aaactcgtcg acgacaagcg
    11041 acagggccgg atctttgacg atgacttggt ggccgttctt tccttcgcgg agaaacaccg
    11101 catagagatg gcgtgtcaac acggtcaacg cgtctggctg gttcgggtcg atgataagaa
    11161 tgcgattgag cagcgccacc cgatcacgca gatcgggaaa cgtcgccgct cgagcggcag
    11221 ccgccgtcag cgtaccgggc tgatcggtcg gcccccacca gaccagcgag tcggggaggg
    11281 ctcggctggt ttccgtcgtg aaggggattt tttcagccat catgccaggg gtttcaggct
    11341 gcgtgatcac cggtaaatta aagacggcgg cgtcgccggg gaagccgtgc tttttgagac
    11401 cggtaaagac cacccattga gtcgtcacgg attttaatgc ccctcgcgca cgtttgatcg
    11461 tgttggcccg tttcacgacg cccgctgcat acgtgaccgg cgaggtgagg ggatcctgat
    11521 aactcacggt caagaagacc agggtggtgg gaacaccgac gatcttgccg ttgggtttga
    11581 ggcggaagga tccggaagga atcgtctgac tcttggccac ctgcacgtgc agaccggttc
    11641 ccggagggga aatccccaac tcgtccatca cgaaggccga ggcaggtgtg cgaggcaatt
    11701 catcgccgaa gaacgccagt gggccggcgc tcggccaaag gatggccata gctacatcat
    11761 cccgtttcag tatggatgca tgggctgcct tgagcgtttg ccagcaggag tcgtacgagg
    11821 gatcgttcgt cggcggatag gattttactc ccgcaggcga ggcggccacg atcctgtact
    11881 gacaagcgaa atccagttga ccgacggtca cgcggtctcc tttggcgatc gcctcggcat
    11941 agcggcgcgc ggtgtccgtg gcaggggtgc tttttgtgac ggtgggcttc tgctgtttcg
    12001 ttggggccgc aacgacaggg ccactgaatc cgagggtgac gaccgtcaga atgctggcaa
    12061 gaaccagcgg gtttgcgatt cgtgtgaagc gacatgccat gacgattacc tgtcctccca
    12121 aacagtgaaa gactctaaaa aaatgcactg taacatggtg tcggaagaat ccgaaagggg
    12181 ctcgggtgca gacctccggc ggtcagggcg ccggcggtgt gagcgagcga ctaaagcgga
    12241 caagcgagcg gatatacgcg aggacggctt gctgatcttc gtcggataac tcgtccttcc
    12301 atgccggcat ggcggtgcga gggcgtccat tcgcgatgat tttcaacagg tcctgatcgg
    12361 atttggccga cgtggcggcg gaaatcaggt ttcccggtcg aggggagaga gacaacgcgt
    12421 tgcgcccgtc cccacggcct tcggatccat gacagtcaag acaccgttgt tcgtagatga
    12481 tccgcccccg ttcccatttc gtttggtcgg attgggccgc gaccgaggtt agaccgactc
    12541 ccaagaggcc gggaatgatg atggcgaatg cggctctcat ccatcctagc ggagtctgtc
    12601 ccatggcccg tcactctacc acaatcattg ccgccatctg atgacccggc atgtcgcaga
    12661 tgtacgaata ttcgccggct cgctccggtg tgaagtgtat ctccactgac ccttcgggag
    12721 ggatgatcgc ccgtttgaat ccttccggcc cgaattccgg cgctccattg ccggtgacgt
    12781 tgagattcac gccagcaaac agctcggaag gcacaaacgc gtgaagctcg ctatcgtggt
    12841 ttctaaagac gagcacggtc ggctgtccat gatgaagagc agcacggtgg ggtatgaaga
    12901 cgcggctttg aatatccacg gcgacgacaa gaggttcggc cgccgtgatc gggggcaccg
    12961 gccaactcag gtataacaag aacaagacgg tccgcgacga gactctccac atctggcaag
    13021 tcatcagggc ccagttcgtc atcatcgggc ctgtgcacga gcagcgcgca agatgttttc
    13081 ccgcaaagcc tggcgatcgg tcggcgagag cgccggggtt ggtcgatgtc gacagagggc
    13141 gaccgtatga cggagtgact ccacaaacac ctcgcagttg ggacaggcac cgaggtggcg
    13201 gcgaatttca tcgcacacat tcgaagccag ttcgtcgtca atgtaggcgg acaatcgctg
    13261 cagaatgtgg agacaccggc ctgtctcgtg cttgtggccg gatcctccct gaaccccggc
    13321 tttcttgcgc gatatggctt ttttcgtcat cggtcgtatc ctcctgatgt ctgcgtcgtc
    13381 gcccgagcat cttcggacgg gccttccgta attccacggg cgctgagttc ccgcctaacg
    13441 aacaagcgag ctcgatggag cctagatttg acggcccgtt cgttcaatcc catcaccgtc
    13501 ccgacttctt tggcgcttag cccttccata tcgcggagca cgaggaccat ccgatatttc
    13561 ctcggaagct tctcgatcgc ctgatcgagg gcctgccgga gctctttgtt ctgaaacgct
    13621 tcttccggtg tgagtccgtc gactgggatt tgaagtcgga attcgccttc cgaggtcggg
    13681 acgaactcat ctagcgaaag ctcccg
//
LOCUS       DATDNW010000092         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_9046,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000092 DATDNW010000000
VERSION     DATDNW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9046"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VI359_02425"
     CDS             <1..320
                     /locus_tag="VI359_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY6973140.1"
                     /translation="ARLSHEGAQLSRLLTDYHGRVAFPFVTIIMVLVGIAMSLRRSGV
                     RGGSMAIGIGQSLAVGFCYWTTHSIAIALGRGGALSPMMAGWTANMLFLSFALYLMLK
                     VRY"
     gene            487..1743
                     /gene="fabF"
                     /locus_tag="VI359_02430"
     CDS             487..1743
                     /gene="fabF"
                     /locus_tag="VI359_02430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY6973141.1"
                     /translation="MPARVVITGLGIVSPIGIGVSEFWKAALAGRSGITAIKSFEPFP
                     MDGYRSRVAGQIHDFVPEQLLTGVLAERVDRYAQFALVAAKEALADSHLQMERENPHR
                     VGVIVGAGMGGMVMGEREITQLYQQQKPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQALQCIRDGRADVVITVGADASITPLVFAGFCSLRALSTKYND
                     RPDQASRPFDKGRDGFVMGEGAGALILESAAHAKKRKAHVYAEIAGYAATSEAYHMVI
                     PREDGEEVATTMRLALADAGITPAQVDYINAHATSTTIGDAVEAKALRQVFKARAEKI
                     AISATKSLIGHTLGAAGAIGAIASVLSLHTGRIHPTANYDDPDPACALPGISRSVQER
                     RVNIALLNAFGFGSNNAAVVLKQITH"
     gene            1755..2060
                     /locus_tag="VI359_02435"
     CDS             1755..2060
                     /locus_tag="VI359_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6973142.1"
                     /translation="MATTTTTAIKISTALAEYLKRDPASITEAHHLRDDLGLDSMAVI
                     ELLYRIEETFDIQIPDEDLAGLITVGHVINYVDKRLGSTRNTGTSKKPSKSTSKKRS"
     gene            2068..>3102
                     /gene="lpxD"
                     /locus_tag="VI359_02440"
     CDS             2068..>3102
                     /gene="lpxD"
                     /locus_tag="VI359_02440"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY6973143.1"
                     /translation="MASPHLQTPISLIQIHQVTGGELVGPPDVAITNLASLNEARSTD
                     LSFITGDRHLKTNVSIQAGALLAHRRLTELRCPHIVLSNPLLAFARVAQTFFCPAVPP
                     RGIAPGTVKGAEVQIGSNASIWPGVTLGDRVRIGSSVTLYPGVFVGDDSTIGDHTVLY
                     PNVVVREGCHIGTRVIVHSGTVIGSDGFGYVQDQGKHYKIPQLGGVIIEDDVELGANV
                     TVDRATMPRDYTTIKQGTKVDNLVQIAHNVTIGEHSIIVAQVGIAGSTTIGHHVLIGG
                     QAGLADHIHVGDQVMIASRAGVNRNLESNQIVSGAPVMPHETWLKAQAVIPRLPELRH
                     LVHSLEERIKR"
BASE COUNT          680 a          881 c          838 g          703 t
ORIGIN      
        1 ccgcacgcct ttctcacgaa ggcgcacagc tctctcgatt gctcacggac taccatggtc
       61 gtgttgcgtt tccgttcgta accatcatca tggttctcgt cgggatcgcc atgagtctcc
      121 ggcgaagcgg tgtccgaggg ggaagcatgg cgattgggat cggccagtcg ctcgcggtag
      181 ggttttgcta ttggactacc cactcaatcg ctatcgccct cggccgcggt ggggcattgt
      241 cgccgatgat ggccggctgg acggccaata tgctcttttt gagttttgcg ctctatctga
      301 tgctcaaagt acgttattag caaaagcctg ggaaaaacct ctgctgtgct ttcatcatcg
      361 aagctgtcca ttgtgcatcg gaacgatatc ccggcctgcc tccctgaacc tcgtctggcc
      421 ggttgactgt atgacaggct tccggtaagt actgaagctg tttcttttgg aaaggattgg
      481 tcgctcatgc cggctcgagt agtcattact ggactgggta ttgtttcccc gatcgggatc
      541 ggtgtctcag aattctggaa ggccgcgctg gctggccgct caggaatcac cgcgatcaag
      601 tcgtttgagc catttcccat ggatggttat cgttcacgtg tagccggtca gatccatgat
      661 tttgttcctg aacagctgtt aactggtgtt cttgccgaac gagttgaccg gtacgcacaa
      721 ttcgcgctcg ttgcagccaa ggaggcactg gcggattctc atcttcagat ggaacgggaa
      781 aatcctcatc gtgtcggtgt gatcgtagga gcaggcatgg gcggcatggt gatgggagaa
      841 cgagaaatca cccaactata ccaacaacag aaacctcacc gggtccatcc gaacttcatc
      901 ccggtgatta cgttaaactc tgcctccggc attgtcgcga tggcctatgg ggccaaaggt
      961 cctaacctga ctatttctac ggcctgctca tcgagcgccc acgcgctcgg acaggcgctt
     1021 cagtgcattc gcgacggtcg tgcagacgtc gtgatcacag tcggcgccga tgccagcatt
     1081 acgccgttgg tattcgccgg attctgttct ctccgcgctc tttccacgaa atataacgat
     1141 cgaccggacc aggcatcacg cccctttgat aagggccgtg acggattcgt aatgggcgaa
     1201 ggggcaggag cgctgattct tgagtcagcg gctcacgcga aaaaacggaa ggcccatgtc
     1261 tatgcggaga tcgcggggta tgccgccacc agcgaggctt accatatggt cattcctcga
     1321 gaagatggag aagaagtggc aaccacgatg cggttggctc tcgcagacgc cggtatcaca
     1381 ccagcgcaag tggactacat caacgcccat gctacctcga ccaccattgg tgatgccgtc
     1441 gaggcgaaag cacttcggca ggtatttaaa gcccgcgcag agaagatagc cattagtgcc
     1501 acgaagtcgt tgatcggcca taccctcgga gccgctggtg cgattggtgc cattgcttcg
     1561 gtcctctccc ttcacaccgg acggattcat ccaaccgcca attatgacga tccagatccg
     1621 gcctgtgccc taccaggcat ttcacgttcc gtgcaggaac gtcgcgtcaa catagctctc
     1681 ctcaacgcat ttggatttgg cagtaacaac gccgctgtgg tattgaaaca gatcacccac
     1741 tagagaacag agccatggcc accacaacaa caactgcaat caagatcagc accgctctgg
     1801 ccgaatacct taagcgtgat cccgcttcga tcaccgaggc ccatcattta cgagatgatc
     1861 taggcctcga ctcaatggcc gtgattgaat tgctctatcg cattgaagag accttcgaca
     1921 ttcaaattcc cgacgaagat ctcgcgggat tgatcacggt ggggcacgtg atcaactacg
     1981 tcgacaagcg gctgggcagt acgcggaaca cgggcacatc aaagaagccc tccaagtcga
     2041 cgagcaaaaa acgatcgtaa cgcattcatg gcttctcctc atcttcagac tcccatctct
     2101 ctcatccaga ttcatcaagt gacgggtgga gagctcgttg ggccccctga cgtggcaatc
     2161 acgaatcttg cgagtctcaa tgaggcacga tccacggacc tatcatttat cacgggcgac
     2221 cgccacctca agaccaacgt atcgattcag gccggcgcgc tgcttgctca tcgtcgactg
     2281 acggaactac gctgcccaca cattgttctc tccaaccccc tgcttgcgtt cgctcgtgtc
     2341 gctcaaacat ttttttgtcc ggccgttcct ccccgtggca tcgcgccggg cacggtcaaa
     2401 ggagcggagg tgcagatcgg ctccaacgcc tcgatctggc ctggcgtcac ccttggtgac
     2461 cgagtcagaa tcggctcttc tgtgaccctc tatcccgggg tttttgtggg agatgattcg
     2521 acgatcggcg accacacggt actctatccc aatgtcgtcg ttcgcgaagg gtgccacatc
     2581 ggtacccgcg tgatcgttca tagcggtacg gtcattggca gtgacgggtt cggttacgta
     2641 caagaccagg gcaagcatta caaaattccg caacttggcg gggtgatcat tgaagatgac
     2701 gttgagttgg gcgccaatgt gacggttgat cgcgcgacaa tgccgcgtga ttacacgacc
     2761 atcaaacaag gcaccaaagt cgataatctt gtccagatcg cacataacgt cacaatcggc
     2821 gaacattcca ttatcgtcgc gcaggttggg atcgccggga gtaccacgat cggtcatcat
     2881 gtgttgatcg gaggacaggc tgggcttgcc gaccacattc acgtcgggga tcaggtcatg
     2941 attgcgtctc gagcaggagt taaccggaat ctagaatcga atcaaatcgt ctccggcgct
     3001 ccggtcatgc cgcatgaaac ctggttgaag gcccaggcag taatccctcg tctcccggag
     3061 cttcgacatc tggtccatag cttggaggag cgaatcaaaa gg
//
LOCUS       DATDNW010000093         4209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_213882, whole genome shotgun sequence.
ACCESSION   DATDNW010000093 DATDNW010000000
VERSION     DATDNW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213882"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1303)
                     /locus_tag="VI359_02445"
     CDS             complement(29..1303)
                     /locus_tag="VI359_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY6973144.1"
                     /translation="MRGLTLIHPVEPAQLEKETLRIYEICDGCRRCFNLCPSFTTLLD
                     GIDKYEGHVAKLTPTDHHRIVDECYYCKLCYNHCPYTPPHQYALDFPRLMVAWKKHLA
                     ATRGVRWRDRLLLKTDLMGKVAGAFAPVVNWTLGLKVLRWLAQLLVGVHRDRHVMSFS
                     SETFARWWERRRGAAVPASAVRKVALFGSCLVNYQATDVGKATVQVLEKNGVHVAVPE
                     QRCCGMPSFDIGDTATIQQAAAANTASFLPWVDQGYDIVVPVPSCSLMWKREYPEIVG
                     GAAVKRVAERTFDVCEYLMRLKREGALATDFQKQPGRVAYQVPCHLRDQNIGFKSKEL
                     METAGAQVEVIEKCSGHDGSWSAKTEFFPLSMIIAMKAVRAIEQAPADIVASDCPLAG
                     LQLDQAGAMAHTGGRATKHPIQIVRDAYGLPS"
     gene            complement(1417..1845)
                     /locus_tag="VI359_02450"
     CDS             complement(1417..1845)
                     /locus_tag="VI359_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY6973145.1"
                     /translation="MGKSLKGTKSHENLKGAFAGESQANRRYLYFARRADIEGFPDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVAIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFAELAEWFETLAKAERSHANRFTKGLDSLKQG"
     gene            complement(1982..2395)
                     /locus_tag="VI359_02455"
     CDS             complement(1982..2395)
                     /locus_tag="VI359_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973146.1"
                     /translation="MPSLNVQNPGMPDLQFVLFVSALCTSNLETLNIPAELRTAVFDR
                     CWTLMNTDPPPTNPKERVLDLREGTELTLEACVSMIRSLFSAAGIATLTWDHPVSDPT
                     RESSPAAKPLIERLGQLYPEPPDIVDPGPAGAGRA"
     gene            2488..2763
                     /locus_tag="VI359_02460"
     CDS             2488..2763
                     /locus_tag="VI359_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY6973147.1"
                     /translation="MVTITLMGQLQTADGERDLACEVPSPMSVRQVIQRQGVQLRHLL
                     QLIREKKVLVTINKKIASQDSMVQDGDAIRLVGHDGMGGSGLGPSLS"
     gene            complement(2889..3176)
                     /locus_tag="VI359_02465"
     CDS             complement(2889..3176)
                     /locus_tag="VI359_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY6973148.1"
                     /translation="MAKSMTKSQIAEHMAQKTGLTKKAAVQALDDFAALAYREAKNVF
                     VVPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVSKAAKDAILGRK"
     gene            3464..4021
                     /locus_tag="VI359_02470"
     CDS             3464..4021
                     /locus_tag="VI359_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973149.1"
                     /translation="MAKELPILPSTAQPADPAQADQSTYSRVFCQKENSPPLRLLLEF
                     LKSRGQTPITPEKLDESALDDWAWLQIPLGYSRGRKPINVFCVRDRGSFKDLFEQEQG
                     YFKETLSIFDDVEADLMLEYVTRARFIITTQMNTTDITDEGYDFNGWILQFYQEQCNG
                     VVQVDGQGFYSPGGQLVVDLSTPTD"
     gene            complement(4001..>4209)
                     /locus_tag="VI359_02475"
     CDS             complement(4001..>4209)
                     /locus_tag="VI359_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973150.1"
                     /translation="MLDLFSVQDGNGEWSDTTARTPFVAGDSVEEGGAGSIKPLICLA
                     KERRLKGRSGGRVHGRAVLVGGCG"
BASE COUNT          924 a         1253 c         1121 g          911 t
ORIGIN      
        1 gggatcaggt cgtcttgcgt caacatcttc atgacggaag cccataggca tcgcgaacga
       61 tttgaatcgg gtgcttggtc gcccgtccac ccgtatgggc catcgcccca gcctggtcaa
      121 gctggaggcc tgccagggga cagtccgatg ccacgatatc ggctggagcc tgttcaatcg
      181 cccgcaccgc tttcatcgca atgatcatcg agagaggaaa gaactccgtc ttggcactcc
      241 aagacccatc atgccctgaa catttctcga tgacttcaac ctgcgcgccg gccgtctcca
      301 ttaattcttt cgatttgaag ccgatgttct gatcccggag gtggcacgga acctgatacg
      361 cgacgcgtcc cggctgcttc tgaaaatcag tcgccaaggc accttcccgc ttaagtctca
      421 tgagatattc gcagacgtcg aacgttcgct cggcaactcg cttgacagca gcgccgccaa
      481 cgatttcagg atactcccgc ttccacatca agctgcaact gggaaccggc acgacaatgt
      541 catacccttg gtcaacccac ggcagaaaag acgcagtgtt cgcagcagcc gcttgctgta
      601 tcgtcgccgt gtcaccgatg tcgaacgatg gcatcccaca acaacgctgt tccggtacgg
      661 ccacatggac gccgttcttc tccaaaacct gcaccgtcgc cttgcctaca tccgttgcct
      721 ggtaattcac caagcaactt ccaaacaatg ccacctttcg tactgcagaa gctggcacag
      781 cggcacctct tcgtctttcc caccaccggg caaaggtttc cgacgagaag gacatcacgt
      841 gtcgatcacg atggactccg actagcagct gggcaagcca tcgaaggacc ttcaagccaa
      901 gtgtccagtt gacgacgggt gcaaaggcac cagctacctt acccatgaga tccgtcttga
      961 ggagcaaccg atcacgccag cgcacacccc tcgtcgcggc cagatgcttc ttccatgcga
     1021 ccattaagcg ggggaaatca agggcatatt ggtgcggggg ggtataggga cagtggttat
     1081 agcagagctt gcagtaataa cactcgtcga caatccgatg atgatcggtt ggcgtcagct
     1141 tcgcaacatg gccctcgtac ttgtcgatcc cgtcgaggag cgtggtgaag gagggacaca
     1201 ggttgaagca ccgacggcaa ccgtcgcaga tttcataaat acgcagggtc tctttttcga
     1261 gttgtgcagg ctcgaccgga tgaatcagag tgaggcctct catgtttctc ccgcagaaag
     1321 cactcagcgg tcagccatca gcatctggga agagtgggct cctcatcccg ctttgctgaa
     1381 aaccgaccgc tgagtgctga ttgctaccag ctcttttcag ccttgcttca agctatccag
     1441 ccccttcgtg aaccggtttg catgagaccg ttccgctttg gccaaggtct cgaaccattc
     1501 ggccagttca gcaaagcctt cgtcccgagc cgtcttcgcc ataccgggat acatctgggt
     1561 gtactcgtac gtctcaccct caatcgccga cttcaagttc gcctcggtat tgccgatcgc
     1621 cactcccgtg gccggatcgc cgacttcttt caagaagtcc agatggccga atgcgtggcc
     1681 ggtctcggct tccgaggtat cccggaacaa ccctccgatg tccggaaagc cttcaatatc
     1741 ggcccgtcgc gcaaaataca gatatcgccg gttggcttgt gattcacctg caaacgcgcc
     1801 cttcaggttt tcatggctct tagtgccttt caggcttttc cccattgccg cctcctccta
     1861 ccctccgaat cagtgacgta tgaacacgta gactcagccc tccggcatat aacacaggca
     1921 tcctctccgg atcaacgcct ccgcggagac gaacttgctc gattcgtcca tttcgctccg
     1981 atcaagctcg cccggcccct gctggcccgg ggtcgacgat atccggcggc tcggggtaca
     2041 attgacccaa tcgttcgatg agtggtttgg cggctggcga gctttcacga gtgggatcac
     2101 tgaccggatg gtcccaggtc aaggtggcaa tcccagcggc cgagaacaac gaccggatca
     2161 tcgacacgca ggcctctaag gtcagctcgg taccctcacg aagatcgagc actcgttctt
     2221 ttggattcgt gggtggagga tcggtgttca tgagggtcca gcagcgatcg aaaactgctg
     2281 tacgtaactc tgcaggtata ttcagcgtct cgagatttga ggtgcagagt gccgagacaa
     2341 agagcacaaa ttgcaaatcc ggcatccctg ggttctgcac attcagtgat ggcatgatcc
     2401 aatattatcg atggcggatt ggagagagtc aacgttacaa ctcgacctga gtcttgtcct
     2461 cgttttgaaa gaggagtgtc tctgacaatg gtcacgatca cgctcatggg ccaattgcaa
     2521 acggccgacg gggaacgaga ccttgcctgc gaggtcccgt cgcctatgtc tgtccggcaa
     2581 gtcatacagc ggcaaggagt ccaactccgc cacctgcttc agctgattcg cgagaaaaaa
     2641 gtgttggtga cgatcaacaa gaagatcgcc agccaggatt cgatggtgca ggatggcgac
     2701 gcgattcggc tcgtcggcca tgacgggatg ggaggcagcg ggctcggtcc gtcattgtcg
     2761 taacgagagg ccagacacac caaaggccgg ctcccttcga ggagagccgg cccttggtac
     2821 ttcattcggc acagataagg ccggaagaag gtgtgaggcc gtacatcagc ccctggacgg
     2881 gatccagctt actttctgcc gaggatcgcg tccttcgccg ccttcgacac gcggaacttg
     2941 accacccgct tggccgggat cttgatgggt tcgccggttt gcggattccg ccccatgcga
     3001 gccttccggt tggccaacac gagcttgccg atccccggga ccacgaagac attcttcgcc
     3061 tcccgatagg ccaaggcagc aaaatcgtcc aacgcttgga cggctgcttt cttggtcagc
     3121 ccggtcttct gggccatgtg ttcggcaatt tgcgacttcg tcattgattt agccatgcga
     3181 gaacctcctt acagaatgag tgaaagagtg aaatgcagtg aatcgccctt ttgacgttgc
     3241 tctgactcga tggtggtccg taatcaggct gtcgtgtctg ctgaagcggc gaaaacctgc
     3301 gggagtctag ccaagcgctc ctatgctgtc aaccggaaaa acacgcatct ggcgtaggaa
     3361 aatgcctgca ccgcaccctt gcagtttcga gaccaggcag cgtagagtat tgtcattgta
     3421 ccagtgtgtg acgtaggaat tcacatagag cgaccgcgct agcatggcga aagaattacc
     3481 cattctccca tcgacagccc agccggctga ccccgcacag gcagatcaat ccacctattc
     3541 tcgcgtgttt tgccagaaag agaattcccc tccgctgcgg ctcctattgg aatttctcaa
     3601 gtcacgcgga cagaccccca ttacccctga gaagttggac gagagcgcgt tggacgattg
     3661 ggcgtggctg caaatccctc tgggatacag tcgaggacgg aagccgatca acgtgttctg
     3721 cgtacgggac cgtggctcgt tcaaagacct cttcgaacaa gaacaaggct atttcaaaga
     3781 aaccctgtcc atcttcgacg atgtcgaagc ggatctgatg cttgaatatg tgaccagggc
     3841 ccgtttcatc atcacgacgc agatgaacac gacggatatt acggacgagg gctacgactt
     3901 caacggctgg atcctgcagt tttatcagga acaatgcaac ggcgtcgttc aagtagatgg
     3961 ccaggggttc tattcgccag gcgggcagct tgtggtcgat ctatccacac ccaccgacta
     4021 gcacggcacg gccgtgaacc cgtcctccgg atcgcccctt cagccgccgc tctttcgcca
     4081 ggcagatcag tggtttaatc gagcccgcgc ctccctcctc gacactatcc cctgccacaa
     4141 agggtgttct cgctgttgta tcggaccatt ccccattacc atcctggaca gagaagagat
     4201 ccaacatgg
//
LOCUS       DATDNW010000094         4846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_624455, whole genome shotgun sequence.
ACCESSION   DATDNW010000094 DATDNW010000000
VERSION     DATDNW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624455"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VI359_02480"
     rRNA            complement(<1..612)
                     /locus_tag="VI359_02480"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            918..1955
                     /locus_tag="VI359_02485"
     CDS             918..1955
                     /locus_tag="VI359_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973151.1"
                     /translation="MSWHETPFPLRRRTLLKALGLGSLAGMTGGCDAMGSVFGRMFAM
                     PPRETTYFTPNSKFYVVNYADSAVSMSREINIEQWKLQIKGEVKTPMALGWRDILNRD
                     SYDQVQTLMCIDTLPGGDSLGNATWRGISLKKLLQEAGADEETARDVIFRGIDGYDDS
                     IPFKRAMQDDVMLAFLMNGEKLPKEHGFPIRLLVPGLYGIKNVKWIVEIEVYPGDYLG
                     YWQRKGWTDDGTIKVFSRIDSPGHYQTLKGSEQTFRGIAFGGPNSVTKVEVSFDAGRT
                     WNDCEIGKPMSPYSWVIWNYTWKPPKQGKFQVVVRATDTKGQLQIAELIRPQPAGASG
                     YHTIIADVEQI"
     gene            2086..3258
                     /locus_tag="VI359_02490"
     CDS             2086..3258
                     /locus_tag="VI359_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY6973152.1"
                     /translation="MALQQPEPKNLAVLEPETPRSVIIKELGIPAKTEEHTENKVDVF
                     QVSQGYTKELKMLRAGLHLTADAFTFGLWELYGISIEKGIRPPDLSLEVHYDANDRMT
                     TFKVLSSDNLVLGKSPADVPPPATARPRISSVTPVLMPQPITPYPQRLAVIVPHGPNT
                     SYILSGLDLALAYLRTFHPTMAIVERERLEPVTQELVLQHAGIVQDDTVARVGGWKGA
                     DSLLIVQIEQISAEHLQAVAQRGGNIAHSVEIRLTHVETGLLLFRQTTLAQVRVPPPT
                     SNQTWPDEVIDDAQRETLRTAYTHSLAALAAAFGDNPLGLVPDMYSRGGGIRLLGLLH
                     GGPGHNAGLQEGDRILEVDGVPYRSVTQRITLPAKLLVERRNQREEVRVDALNFAK"
     gene            complement(3402..4568)
                     /locus_tag="VI359_02495"
     CDS             complement(3402..4568)
                     /locus_tag="VI359_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973153.1"
                     /translation="MLHFIPSLARQFIAASMSLLLVLVVVMGCQRADLRKQSGRVCAD
                     VEAEIDLSKRIATLNLLSEASIAHCDDVVVTYGAQARSEFRHKAFSITRETANVFVPD
                     GTFIEYIMESYERGYLSILLAISYIRLHKTEDAKVELRDLDHELFAPIYNYGEDPVNL
                     LFSAVLWEQLGDVGEAHVDWLRLRDFRIFAMREDPLLRRFAEERVSQIDNREGAGPEW
                     HVYQVGRFPQLDWDLRFSNSTSGYFSITPKEPFMASCVSSTGVLVSTESWFNKIAIRH
                     SHAYHPLLNVQTWIRLPIGVVYSLIPVVAGAGIMVGGCMLDAAGNGKGALCQLSVVGG
                     VALMRTAPDVLEGSLRPDLRHWQEVPAAIVVTRASEANQEPCMAKLKGGVQRLL"
BASE COUNT         1099 a         1444 c         1261 g         1042 t
ORIGIN      
        1 cctggagatt tcacagaggg cttaccgaac cgcctacgct ccctgtacgc ccagtaaatc
       61 cgaacaacgc ttgccacctt cgtattaccg cggctgctgg cacgaagtta gccgtggctg
      121 cttctggagg taccgtccga acggttgccc gtcccatctt ccctcccgaa aggggtttac
      181 aatccgaaga ccttcgtccc ccacgcggcg tcgctgcgtc aggctgtcgc ccattgcgca
      241 atattcctta ctgctgcctc ccgtaggagt ctggcccgtg tcgcagtgcc agtgtggctg
      301 atcgtcctct cagaccagct acccgtcgaa gccttggtga gccattacct caccaacaag
      361 ctgataggac ataagcccat ccaagagcgc gatacccacg gtatcgcttt cctccctgaa
      421 ccgcagtcca aggagcgtac gcggtattag ctaacctttc ggctagttat tccccactcg
      481 agggtaggtt acctatgtat tcctcacccg ttcgccactg tacatgtgta ttgctacaca
      541 ttctcgttcg acttgcatgt attaggcgcg ccgccagcgt tcgttctgag ccaggatcaa
      601 actctcatat aggtgttgtt ttcaaattgg accaagggcc accacttcaa tacaccttac
      661 accttgctca tcgtcctcta ttcagttgtc aaagagcgag aaaactcatc acgcgagccg
      721 cgcactatac aactcgcgcg acagcgtgtc aagggacgtg ctcggagctc tacgcttcgg
      781 gtagtttccc tttggaaccg caccgtgact cgcgtcaggc gcggtcaaca ggcaggaaca
      841 taaaaccgct aacgagcggt aagatagcaa gaccttccgc gagagtcaag tctcatttcc
      901 gtgtataatc tgcgatcatg tcctggcacg aaacaccgtt ccccctgagg cgacgcaccc
      961 tcttgaaggc gctgggcctg ggctcgctcg ccggcatgac cggcggttgc gatgccatgg
     1021 gctctgtctt cggccgcatg ttcgccatgc cgccgcggga gaccacgtac ttcacgccga
     1081 acagcaaatt ctacgtcgtg aactacgccg acagcgccgt ttccatgtcg cgcgagatca
     1141 acatcgaaca atggaaactg cagatcaaag gagaagtgaa aacgccgatg gcactcggct
     1201 ggcgcgacat tttgaatcga gattcttacg accaggtcca aacgctgatg tgtatcgata
     1261 cattgccagg cggtgacagc ctgggcaatg caacatggcg cggcatctcg ttgaagaaac
     1321 ttctccaaga ggcgggtgcc gacgaggaaa ccgctcggga tgtcatcttc cgcggcatcg
     1381 acggctacga cgacagcatt ccgtttaaac gagccatgca agacgatgtg atgctcgcct
     1441 ttctcatgaa cggcgaaaag ctgcccaagg agcatggctt tccgatcagg ctgctggtgc
     1501 ccgggctgta cggcattaag aatgtgaaat ggatcgtgga gatcgaggtc tatcccggag
     1561 actatctcgg ctattggcag cgcaaaggat ggaccgacga cgggacgatc aaagtgtttt
     1621 cacgcatcga cagtccggga cattatcaaa ccctcaaggg ctccgagcag acgtttcgcg
     1681 gcattgcctt cggcgggccg aattccgtca ccaaagtcga ggtcagcttc gatgccggcc
     1741 gaacatggaa cgactgtgag attggaaaac cgatgtcgcc ctactcctgg gtcatctgga
     1801 actacacctg gaaaccgccc aagcagggga agtttcaagt cgtcgtgcgt gcgacggata
     1861 caaaagggca gttgcagatt gcagaactga taagaccaca gccggcggga gcgtcgggct
     1921 atcacacgat catcgcggat gtggagcaga tctgatttgc gcggtgtaaa atcagccctt
     1981 gccataacag gaaacggaga actttcgttt tttccgatac atgatacgct gaggtggtgt
     2041 tcactcgcgg tcctactgtt cggtctcccg ggctgttcgg tcgtcatggc cctccagcag
     2101 ccagagccga agaatctcgc cgtcctcgaa cctgaaactc ctcgatccgt catcatcaaa
     2161 gaacttggta taccagcgaa aaccgaggaa cacactgaga acaaagtgga tgtgttccag
     2221 gtgtcccaag gctacaccaa ggaactcaaa atgttgcgtg cggggctcca cctgaccgca
     2281 gatgctttta cctttggatt gtgggagctc tacggaatca gcatcgagaa aggaatccgc
     2341 ccgccagacc tttctttgga ggtgcactac gatgccaatg accgcatgac caccttcaaa
     2401 gtcctttcga gtgataatct ggtgctcggg aagtcaccgg cagacgtgcc gccgcccgct
     2461 accgcacggc cgcgcatctc gtctgtcaca ccggtgctga tgccgcaacc tatcaccccg
     2521 tatcctcaac gactcgccgt gatcgttcca cacggcccca acacctcata cattttatcc
     2581 ggccttgacc tcgccctcgc ctatctccgc acctttcacc ccaccatggc catcgtggaa
     2641 cgcgaacgtc tcgagcccgt cactcaagaa ttggttctcc aacatgcggg aatagtccag
     2701 gatgacaccg tggcgcgtgt cggtggatgg aagggcgcgg actcattgct gatcgtacag
     2761 attgaacaga tctcagctga gcatctccaa gccgttgccc agcgtggagg caacatcgct
     2821 cattccgtcg aaatccgcct cacgcatgtg gaaacgggcc tgctgctctt tcgacagaca
     2881 accctggcac aagtccgagt ccccccgccg accagcaatc agacctggcc ggacgaagtt
     2941 atcgatgatg cacagcgcga aaccctgcgc acggcataca cgcactcatt ggccgcattg
     3001 gcagccgctt tcggcgacaa ccctctggga ctcgtgccgg acatgtattc tcgcggtgga
     3061 ggcattcgct tgctgggact ccttcatggc ggtcccgggc acaatgcggg attacaagaa
     3121 ggggaccgca tcctcgaagt cgacggcgtt ccatacagga gcgtgacaca acgcattaca
     3181 ctccccgcga agctcctggt cgagcgtcgc aatcaacgcg aggaagtgcg tgtcgacgcc
     3241 ctgaatttcg caaagtagtc ctgagtcaac cccggcaggc gagggcgtgc agattttcta
     3301 cgatcatcgc taatctggcc gaactctaaa gccgcacgaa tcaatcacct tgcccacctt
     3361 gattttagaa agtcgttcgg ttcaactttg acatctgggc tttacaacag ccgctgaaca
     3421 ccacccttca atttcgccat gcagggctct tgattcgctt cggatgctct cgtaaccact
     3481 atcgctgctg gcacctcttg ccagtgccgc agatccggac gcagcgatcc ttccagcaca
     3541 tcgggcgccg ttctcatcag cgccactcca ccgactactg aaagctggca gagtgcgccc
     3601 ttgccatttc ctgcagcatc cagcatgcaa cctccgacca tgattccagc tcctgccaca
     3661 actgggatca gactgtacac gacaccaatc ggcagacgaa tccacgtttg cacgttcaac
     3721 agcgggtggt atgcatggct atggcggatc gcaatcttgt tgaaccagct ttccgtcgat
     3781 accaagacgc ctgtgctcga cacacaggat gccatgaacg gttctttggg ggtgattgaa
     3841 aaatagccgc tcgtcgaatt gctgaatcgt agatcccagt cgagctgagg aaatcgcccg
     3901 acctggtaga catgccattc cggaccggcc ccctctctgt tgtcgatttg gcttactcgc
     3961 tcctcggcaa atcgacgaag cagcggatcc tcccgcatcg caaagattcg aaaatctctg
     4021 aggcgaagcc agtccacgtg agcttcaccg acatccccga gttgttccca caaaacggct
     4081 gagaacagaa ggttgaccgg atcctctcca taattgtaga tcggtgcgaa cagttcatga
     4141 tccaagtctc tcagctctac cttggcgtcc tcggttttgt gcagtcggat gtaactgatc
     4201 gccagaagga tactgagata gccccgctca taactctcca tgatgtattc gatgaacgta
     4261 ccgtcaggaa caaatacgtt tgccgtttcc ctggtgatcg aaaacgcctt gtgccgaaat
     4321 tcggagcgag cctgtgctcc ataggtgacg acaacgtcgt cgcagtgcgc tatggaggcc
     4381 tctgacaaca ggttcagtgt ggctattcgc ttagaaagat cgatttctgc ctcaacgtcg
     4441 gcacagaccc gtccactctg cttgcgaagg tccgcacgtt gacagcccat gacaaccacg
     4501 aggaccaaca ggagactcat agatgcggcg atgaattgtc gagccaagga agggatgaaa
     4561 tgaagcataa ctttctaatg tcgtcgtttg acttgaagac tctggcagcg cagtgtatcc
     4621 aagcatccgg agagtttcca gacggtgacg accagggacg ttctgatggt ttcggtttcc
     4681 gtgcgctgga gagtaatcac cccggactca ccgatggaga gtgtacggtt gcgcaagctg
     4741 ctgcctgaga aaatctccag ccttgcggac gtccgttcga gaggctaatg cactttcata
     4801 ggtcccgctc gtctctgtgg tgaaaaccag gcgccaccga cccttg
//
LOCUS       DATDNW010000095         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_692559, whole genome shotgun sequence.
ACCESSION   DATDNW010000095 DATDNW010000000
VERSION     DATDNW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692559"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VI359_02500"
     CDS             complement(<1..823)
                     /locus_tag="VI359_02500"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973154.1"
                     /translation="MTESKPTPASSDGNIKERNSTRLASSELDRRWLHLRPVWIVLIL
                     LLPCLALTFYYSQTIVPGGEQTGDSFLPTTSYAFVLLLINLDLIGFVVLLLLLSRNLV
                     KAYFERRHRLLGSGFRNKLVAAFIGFSLIPTVLLAVVASGLVNKAVDVWFSDQIERVM
                     RDSYEVARMQHAGHITLAVNSARAIGHEIYREDMLLAEQRDLLVAAMARKRMEFGVAG
                     IEVYSAKLETLTKAVDPEIPSMVLDLPVGQLVLQAINGKQEVNTVQEAQTGRLVRA"
     gene            complement(965..1369)
                     /locus_tag="VI359_02505"
     CDS             complement(965..1369)
                     /locus_tag="VI359_02505"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEY6973155.1"
                     /translation="MATEKDEGFVIRDRRGGSGTDRPSAATSGMSPAQDHASSPPTSP
                     SSSSQSSGVPVTFASFVISLGSSSLMLMGEQLDPQQSPMPVNLPQAKEIIDLLSVLEE
                     KTKGNLTQDEQTVLRDMLYALRMKYVSLTSKA"
     gene            1521..2435
                     /gene="mazG"
                     /locus_tag="VI359_02510"
     CDS             1521..2435
                     /gene="mazG"
                     /locus_tag="VI359_02510"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY6973156.1"
                     /translation="MSESFSKLVHLMATLRAPNGCPWDRKQTHESLKPYLLEETYEVL
                     ETIDHQDRAKLAEELGDVLLQVIFHSQIAAEAERFTIDDVVERLSDKLIRRHPHVFGN
                     GSTGQQPTTAEQVVHRWEDIKRAERQASGRSASILDDIPKTLPALLRAFQVQARAARV
                     GFDWSHDAAGFDQVLTKIEEEISELRVAVRAPASEPGANDTTTTSSRQDQIIAEFGDV
                     LFSLVNLARFLKVNPEDTLRLATNRFSERFHYIENRAKAAGRTVNELSYAEMDRLWEE
                     AKTRGETLSGSSTPTQPSTSTEDETDER"
BASE COUNT          619 a          730 c          722 g          523 t
ORIGIN      
        1 cagcccgaac cagacggccg gtttgcgcct cttgaaccgt gttcacttct tgcttgccgt
       61 tgatggcctg caacaccagc tgtccgaccg gaaggtccag caccatggag ggaatctcgg
      121 gatcaaccgc cttcgtgagg gtctccagct tggctgaata gacctcgata ccggcgacgc
      181 caaattccat ccgcttgcgg gccatggctg ccacgagtaa atcacgttgc tccgcgagta
      241 acatgtcttc gcggtagatt tcgtgaccga tggcacgggc actattgacc gccagcgtga
      301 tgtggccggc atgctgcata cgggccactt cgtaggaatc gcgcatgacc cgctcgattt
      361 ggtcactgaa ccagacgtcg accgccttgt tcaccagccc actggcgact acggcaagaa
      421 ggaccgtcgg gatcagagag aatccgatga atgccgcgac gagtttgttc cggaatcccg
      481 aacccaagag ccgatgccgt cgctcgaagt atgccttcac caggttgcga gacagaagca
      541 acaagaggac gacgaatccg atcagatcga gattgatcaa aagaagaacg aacgcgtaac
      601 tggtggtagg caagaacgaa tcgcccgtct gttctccgcc gggaacgatc gtctgggaat
      661 agtagaaggt cagcgcaaga cagggcagta gcagaatcaa cacgatccag accggccgca
      721 agtgcagcca ccgccggtct agttcagaag aagcaagccg cgtcgagttg cgttctttga
      781 tattcccgtc gcttgaagca ggtgtgggct tgctttcagt catgccagga ttccgtctct
      841 ctgtgcagga tggttgagct atccctcagg cattcactat atcggaatta cgagagcgcc
      901 tcaaaacatc ttgcggaacg gattggacca gggggcagtc gacaccacgt cagaatgaac
      961 gagattacgc ctttgaagtc aaactgacgt acttcatccg aagtgcgtag agcatgtcac
     1021 gcagtactgt ttgttcatcc tgtgtcaggt tgccctttgt cttctcttct aacacggaga
     1081 gcaagtcgat gatttctttc gcttgcggca aattgacagg catgggagat tgctgcggat
     1141 ccagctgttc tcccattaac atcaacgacg agctgccgag ggaaatcacg aaggaggcaa
     1201 aagtgaccgg aacaccggaa ctctgcgaag aacttgatgg agaagttggt ggggatgagg
     1261 catgatcctg ggcaggcgac attccggacg tggcggccga gggacgatcg gtcccactgc
     1321 ctcctcgtcg atctcgaatg acaaatcctt cgtctttttc agtcgccacg agtcgtttct
     1381 ccgcacgaaa cgcaggcgcg gtaccctacc atagggccta ggggggcgca agccagcgag
     1441 tgtcgttgaa gcgaatctgg aacgaggtat aatgggtccc tctgggtacc acttgagccg
     1501 tacttcacac tgcgacaacc atgtcggaat cgttcagcaa actcgttcac ctgatggcca
     1561 ccttacgggc ccccaatggg tgtccctggg accggaagca gacacacgag tcactcaaac
     1621 cctacttgct cgaagaaacc tacgaggttc tggaaacgat cgaccaccaa gaccgtgcga
     1681 agctcgcgga agaattgggt gacgtactgc tccaagtgat tttccacagt caaattgccg
     1741 ccgaagctga gcgtttcaca atcgacgatg tcgttgaacg actgtcggac aagctgattc
     1801 gccgccatcc ccatgttttc ggaaatggct ctaccgggca gcagccgacc accgcggaac
     1861 aggtcgtaca ccgctgggaa gatatcaaac gagcagagcg ccaagcgtcg gggcgctcag
     1921 cttccattct cgacgatatt ccaaagacgt tgccggccct tctccgagcc ttccaagtcc
     1981 aagcacgggc ggcgcgtgtc ggattcgact ggagccatga tgcggccgga ttcgaccagg
     2041 tcttgaccaa gatcgaagag gaaatttccg aactccgtgt ggcggtgcga gcgcctgctt
     2101 ccgaaccagg cgcgaatgac accaccacaa cgtcctctcg gcaggatcag attatagctg
     2161 aattcggtga tgtgctcttc tcgctggtca atttggctcg attcctcaag gtcaaccccg
     2221 aagacacact tcgtctggcg acgaaccgct tctcggaacg attccattac atcgaaaacc
     2281 gtgcaaaggc cgctgggcga accgtcaacg agttgtctta tgccgaaatg gatcgcctct
     2341 gggaagaagc caaaacccgt ggcgagactc tgtcgggatc atcgacaccg actcagccat
     2401 cgaccagtac agaagacgaa accgatgagc gataatcaag gaccgtacga agaaggtaag
     2461 cgcgccgggg tccatccgct tgtcgtggta ttcggagtcc tgctcggact ctggttgttc
     2521 gtcgcgctca tcgtgccgag ttcaaaaaac aagcaagcca cgggaaccga aggacccaac
     2581 gtcccgctga ttga
//
LOCUS       DATDNW010000096         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_692649, whole genome shotgun sequence.
ACCESSION   DATDNW010000096 DATDNW010000000
VERSION     DATDNW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692649"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..2466
                     /locus_tag="VI359_02515"
     CDS             169..2466
                     /locus_tag="VI359_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973157.1"
                     /translation="MTESKPTPASSEWNVKERNSTRLASSELDRRWLHLRPVWIVLIL
                     LLPCLALTFYYSQTIVPGGEQTGDSFLPTTSYAFVLLLINLDLIGFVVLLLLLSRNLV
                     KAYFERRHRLLGSGFRNKLVAAFIGFSLIPTVLLAVVASGLVNKAVDVWFSDQIERVM
                     RDSYEVARMQHAGHITLAVNSARAIGHEIYREDMLLAEQRDLLVAAMARKRMEFGVAG
                     IEVYSAKLETLTKAVDPEIPSNVLDLPVGQLVLQAINGKQEVNTVQEAQTGRLVRAAM
                     PVAASGRGGEIGGVVVVDAYVPESLLAKMEGIGRQYDEYKQLKAMKNPIKAGAYLFVA
                     VITVLILFGATWFGFYVARSITVPIQRLAEATETIAQGDLSVRIDAKATDEIGTLIES
                     FNRMTEDLQGSKSKIEEANISLRQSNIELDRRRAYIETVVDTIAAGLLSIDKNGVITT
                     FNPSGERILGLWADRLRGRPANEVFKEFGLDLFQTVYDRMLADQRDTLTLEGPVEVEG
                     KFLTIGLHGSRMKDDTSRDLGIVLVFEDLTELIKAQKVAAWQEVARRVAHEIKNPLTP
                     IQLSAQRLRKKFFEKSPDFDKVFDESTNVIVNEVTSLKHMVDEFSKFARLPAPQMAHQ
                     SLHDVIDEVVALYRGAHRDIELTVELDEDLPSLSFDREQLKRVLVNLLDNAIQAMNHK
                     GRVWLTTKYDTKRRRAVVSVADEGPGIAPEDQEKLFVPYFTRKKTGTGLGLAIVRRII
                     TDHEGQIQAGHNQPHGAVFTFELPV"
     gene            2499..>3397
                     /locus_tag="VI359_02520"
     CDS             2499..>3397
                     /locus_tag="VI359_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973158.1"
                     /translation="MSASILVVDDEESILASLSSILEDEGYEVTVGKSGADALRAYTT
                     DPPDLMLLDIWMPDLDGLETLRRVRELVPTAQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQYRLEQENRSLRTKVQRKFELVGQSTAMQQLRQLIETAGPT
                     NSRVLIGGENGTGKELVARAIHEHSARADKPFVAVNCAAIPETLIESELFGHEKGSFS
                     GATSMKRGQFEQADGGTLFLDEIGDMSLNTQAKVLRALQEQQFTRVGGTKLMKVDVRV
                     LAASNKDLLKEIEKGTFRDDLFYR"
BASE COUNT          780 a          854 c         1036 g          727 t
ORIGIN      
        1 tacgtcagtt tgacttcaaa ggcgtaatct cgttcattct gacgtggtgt cgactgcccc
       61 ctggtccaat ccgttccgca agatgttttg aggcgctctc gtaaatccga tatagtgaat
      121 gcctgaggga tagcccaacc atcctgcata gagagacgga atcctggcat gactgaaagc
      181 aagcccacac ctgcttcaag cgagtggaac gtcaaagagc gcaactcgac gcggcttgct
      241 tcttctgaac tagaccggcg gtggctgcac ttgcggccgg tctggatcgt gttgattctg
      301 ctactgccct gtcttgcgct gaccttctat tattcccaga cgatcgttcc cggcggagag
      361 cagacgggcg attcgttctt gcccaccacc agttatgcgt ttgtcctgct tttgatcaat
      421 ctcgatctga tcggatttgt cgtcctcttg ttgctgctgt ctcgcaactt ggtgaaggca
      481 tacttcgagc gacggcatcg gctcttgggt tcgggattcc ggaacaaact cgtcgcggca
      541 ttcatcggat tctctctgat cccgacggtc cttcttgccg tcgtcgccag cgggctggtg
      601 aacaaggcgg tcgacgtctg gttcagtgac caaatcgagc gggtcatgcg cgattcctac
      661 gaagtggccc gtatgcagca tgccggccat atcacgctgg cagtcaatag tgcccgtgcc
      721 atcggtcacg aaatctaccg cgaagatatg ttacttgcgg agcaacgtga tttactcgtg
      781 gcagccatgg cccgcaagcg gatggaattt ggcgtcgccg gtatcgaggt ctattcagcc
      841 aagctggaga ccctgacgaa ggcggttgat cccgagattc cctccaatgt gctggatctt
      901 ccggtcggac agctggtgtt gcaagccatc aacggcaagc aagaagtgaa cacagttcaa
      961 gaggcgcaaa ccggccgtct ggttcgggct gcgatgcccg ttgccgccag tggtcggggc
     1021 ggagagatcg ggggcgtcgt ggtggtcgat gcctatgtgc cggaatcgct gttggccaaa
     1081 atggaaggaa tcggccgcca atacgacgag tacaaacagc tcaaagccat gaagaatccg
     1141 atcaaggcgg gagcctatct cttcgtggcc gttatcaccg tgctgattct gtttggcgca
     1201 acctggtttg gattttatgt cgctcgcagt atcacggtgc cgatccagcg attagccgag
     1261 gcgaccgaga cgatcgcgca aggcgacctg tccgtgcgga ttgatgcgaa ggccacggat
     1321 gaaatcggca cgttgatcga gtcgttcaac cggatgaccg aagacctgca agggagcaag
     1381 tcgaagattg aagaggcaaa catctccttg cgccagagca acattgaact cgatcgtcgc
     1441 cgagcctata tcgaaacggt ggtggacacg atcgcggccg ggttactgtc catcgataaa
     1501 aacggagtga tcacgacctt taatccatcg ggcgaacgta ttttgggact ctgggcagat
     1561 cgattgcgtg gccggccggc caacgaggtc ttcaaggaat tcgggctgga tctgtttcaa
     1621 acggtgtacg atcggatgct ggccgatcaa cgggataccc tgacgctgga ggggccggtc
     1681 gaagtcgagg ggaagtttct caccatcggt ctacatggct ctcgtatgaa ggatgacacc
     1741 agtcgcgatc tcggcattgt gcttgttttc gaggatttga ccgaactgat caaagcacag
     1801 aaggtggcgg cttggcagga ggtggcgcgt cgggtggcgc atgagatcaa gaatccgttg
     1861 acgccgatcc agctgtccgc acaacgattg cgaaaaaagt tttttgaaaa gtcaccggat
     1921 tttgacaagg tgttcgacga atcgacgaac gtgatcgtga atgaggtgac gagcctgaag
     1981 catatggtgg atgagttctc gaaattcgcc cgcctcccgg cgccgcagat ggcccaccaa
     2041 tcccttcacg atgtgatcga tgaggtcgtc gccctctatc gtggcgcgca cagggacatc
     2101 gagctgacgg tggagttgga tgaggatctc ccgtcgttga gttttgatcg ggaacagctg
     2161 aagcgggtgt tggtgaacct gttggacaat gcgatccagg cgatgaatca caagggacgt
     2221 gtgtggctga cgacaaaata cgatacgaag aggcgacgag ccgtggtcag cgtggcagat
     2281 gaagggccgg gcattgctcc ggaagatcag gagaaactct tcgtgccgta cttcacgcgg
     2341 aagaaaaccg ggacgggact cgggcttgcc attgtccgcc gaatcattac ggatcatgaa
     2401 gggcaaatcc aagccggtca caatcagccg cacggagcgg tcttcacgtt tgaactgccg
     2461 gtctaaacca agtcgagaag aaaggatcag aaacatatat gtcggcatcg attctcgtgg
     2521 tcgacgatga ggaatcgatt ctcgcctcat tgagcagcat cttggaggat gagggatacg
     2581 aggtcaccgt gggaaagagc ggagcggatg cgctacgggc ctatacgacg gatccgccgg
     2641 atctcatgct tctggatatc tggatgcctg acctggatgg cttggaaacg ttgcgacgcg
     2701 tccgcgaact ggtgccgacc gcgcaggtca tgatgatgtc gggtcatgga tcgatcgaga
     2761 cggcagtgaa ggcgatcaag ctcggtgcct atgactacat tgagaagccg ttgtctctgg
     2821 agaatgtgac gcttcgggtg aagcatgcct tggaccagta tcgattggag caagagaacc
     2881 ggtcactgcg gacgaaggtc cagaggaaat tcgaactcgt cgggcagtcg acggcgatgc
     2941 aacaactgcg acagttgatc gaaacagctg gacccaccaa tagccgcgtc ttaatcggag
     3001 gagagaacgg cacgggcaag gaactggtcg cacgggcgat ccacgaacac agcgcacgag
     3061 cggataaacc gttcgtggcg gtgaattgtg cggccattcc agagacgctg attgaaagtg
     3121 aattattcgg tcatgagaaa ggatcgttca gcggcgccac atccatgaaa cgagggcagt
     3181 tcgagcaagc cgacggcgga accctctttt tggatgaaat cggcgatatg agcttgaaca
     3241 ctcaggccaa agtcctccgg gctcttcagg agcagcaatt tactcgcgtg ggtggaacca
     3301 agttgatgaa ggtggatgta cgggtcctgg cagcgtccaa caaagacttg ctcaaagaaa
     3361 tcgagaaggg gacgtttcgc gacgatctct tctatcg
//
LOCUS       DATDNW010000097         6216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_424065, whole genome shotgun sequence.
ACCESSION   DATDNW010000097 DATDNW010000000
VERSION     DATDNW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424065"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VI359_02525"
     CDS             <1..340
                     /locus_tag="VI359_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973159.1"
                     /translation="SALDQYQSRLAILSGLFDTFYDQAIPEQEASRLFGRMMEATKRL
                     TQQGYILLCLYPPAPILTRTSRRCLDQLRVQADRVIRVQEEQGLVRLEEEGSELGRSW
                     EVARTALESR"
     gene            343..633
                     /locus_tag="VI359_02530"
     CDS             343..633
                     /locus_tag="VI359_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973160.1"
                     /translation="MGRTLVTFTQLVHQEIARWRRYRRALRAEDQQALDALFAAARQH
                     SAAGAYLARDTPFEVMLLSMLLEHQKHLQRLSRAVVDHDVPSSSPPSHGVAP"
     gene            656..2824
                     /locus_tag="VI359_02535"
     CDS             656..2824
                     /locus_tag="VI359_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY6973161.1"
                     /translation="MVVWLFDDEGSRRRLVDPYEPAFYLAGCHTDIALAARLLTERHI
                     AHRCRAVERRELWDADTIPVCEITIVQPTRFQEAVRLVMGSAPHLRFYQADVALPQLY
                     FYDRNLFPLCRCDADVTSDAIIQAIQASDSPWDTAYRMPPLSIIELMLEGASPNPNHG
                     GVFNLLVRIEDTEWRVEQEDAAEFIQTVNGLFHRYDPDVLLTDWGDSYILPRLIHLAQ
                     LARMPLALNRDTRQSIAVRAPRSYFSYGHMVAQAGSQTLYGRLHLDRHNSFALAETGV
                     AGLFEQARVAKVPIQQMARTTTGTGITSMQLERAHRSGILIPYRKQQAEAFKTAGELL
                     ETDQGGFTYAPLSGYHEHVGELDFASMYAAIMVKFNVSPETVNCACCEHDPAAQVPEI
                     YHHTCRRFQGLVPQTLAPLLAKRGLYKQQLKDATNETRKRIIDQRQTALKWLLVVSFG
                     YLGYKNARFGRIEAHESVTAYSREVLLRAKEIAESRGFRLLHAIVDSLWLQKDDAGRE
                     DYEQLAAAISAQTELPITVEGLYRWIGFLPSRVNRHMPVHNQFVGLFDDGRMKVRGLE
                     IRRSDAPLIVKRAQAEILQKLSQGRTITELKVMIPDMVGIVDEYCCYLREGRASVEEV
                     AIGKTLTQPPERYRHATRTSIAAKELRRRGVSLQPGETIHYVICDSQAALPEDRVRAV
                     AGCDGTIAYDAEAYIQLVHKAALAMLAPLGVTAKELERRE"
     gene            complement(2839..3405)
                     /locus_tag="VI359_02540"
     CDS             complement(2839..3405)
                     /locus_tag="VI359_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEY6973162.1"
                     /translation="MTKSRPDGPLEKQWLLYVGRFGAWALAMALFCIAVNCSSVPRRH
                     LWNAEKGVTLMDLQRTPERYQGKTVILGGVIVHEENKDGRLWLRMKNRPLDDNQRPHL
                     ADDPDSAEAGYYWVAVDAAKIPDHYHEWAQVTVVGQVVPTKPGITSDPVLAAVYLRGW
                     SRLSKEHSVWEIDDPNYTMDAPAGLHGE"
     gene            3564..4109
                     /locus_tag="VI359_02545"
     CDS             3564..4109
                     /locus_tag="VI359_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973163.1"
                     /translation="MCSRGIFAVGIWLTLISTAGCLRVLSLDYQATNSIKGQGPVHVM
                     LFRYQSADEQRVRPHEVETPPRSEAKLFLSGAIGTVFSQALQSELARSGYVIEESSPR
                     MISGVVTRFYLDWTKETERTFELEVTFEVQSEGRQPFSWSCSSVKRGVNMLAEDAILI
                     KAGIADCIERFLVATRVAQAL"
     gene            complement(4200..4275)
                     /locus_tag="VI359_02550"
     tRNA            complement(4200..4275)
                     /locus_tag="VI359_02550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4240..4242),aa:Met,seq:cat)
     gene            4445..5047
                     /locus_tag="VI359_02555"
     CDS             4445..5047
                     /locus_tag="VI359_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973164.1"
                     /translation="MQIILPESCKQALLERFLKAEAMALWAVQSAQVQQIQGVPPHVL
                     PFLRRHEEQEAAHLKEFERRLGVPPKERSRLPRVPRQWSALAVHLLGYEALGLEFAKL
                     LAQVQPDLHSILDDELIHVGFFEKEVAQLLLSGGSLADGARLSAQAWWRRLPRTLDRY
                     LDGASLAPYRSDLRRTILMTIEARFTAVGLLDPVHVPARV"
     gene            5089..5646
                     /locus_tag="VI359_02560"
     CDS             5089..5646
                     /locus_tag="VI359_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY6973165.1"
                     /translation="MSDENHIDEKHVLRCILFRHGIAVEREEWSGKESDRPLTDKGKR
                     RVRRVAAGLKRLDVRPTRILTSPLSRAVETAKVLHDALAVRSPLQLVDELLPDAPPDK
                     MIVLLNDLPPGSYALCVGHEPHLGLTASVLLSGRQTSAFPLKKAGACLIELSLPAKPG
                     QGRLVWWLTPGQLRTIGKETNNGED"
     gene            complement(5643..>6216)
                     /locus_tag="VI359_02565"
     CDS             complement(5643..>6216)
                     /locus_tag="VI359_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY6973166.1"
                     /translation="TRSNLMSKTIEMAKEGNILDRWSFTLLYATDFADRLEHQIIPAL
                     RSGFIVLADRYIYTALARDFVRSGDRKWVRDVFGFALVPDLVCYLRIDVETLVLRVIE
                     TKGMNFWESGMDLRLGGDLYDSFKKYQSLLIEEFDKMTEEFKFEVIDARKSPEEIQEE
                     LQNKIRPLLSNHLRSPVLLSDSTAHPSPTT"
BASE COUNT         1310 a         1673 c         1832 g         1401 t
ORIGIN      
        1 gtcggcgctc gaccagtatc agtcgcgcct tgcaatcttg tccggtctgt tcgacacttt
       61 ttatgaccaa gcaattcctg agcaggaggc ctcacggctt tttggtcgta tgatggaggc
      121 gaccaaacga ttgacacagc agggctatat attgctgtgt ttgtacccgc cggctccgat
      181 cctgacacgc accagccggc gttgcctcga ccagttacgc gttcaggctg atcgtgtcat
      241 tcgtgtgcag gaagaacagg gcctggtgcg gcttgaagag gaaggcagcg aattgggccg
      301 atcgtgggaa gttgctcgca ccgccttgga gtctcgatag cgatggggcg gacgctcgtc
      361 acgtttacgc aattagtaca tcaggaaatc gcacgttggc gacgctatcg acgtgccctg
      421 cgcgcagagg accagcaggc attggacgca ttgttcgctg cagctcgaca acattcggcg
      481 gccggtgcgt atctggcgcg cgatacgccg ttcgaggtga tgctgctatc gatgctgttg
      541 gaacatcaga aacatcttca acgtctatct cgtgccgtgg tcgaccacga tgtcccgtct
      601 tcctctccac catctcacgg ggtggctcct tgatgtctat cccgttcatg acggcatggt
      661 ggtatggctt ttcgacgatg aggggagccg gcgtcgcctt gtcgatccct atgagccagc
      721 tttctatctc gccggttgtc acaccgatat cgcgctagcg gcccgcctcc tcacggaacg
      781 ccacatcgcc caccggtgcc gcgctgtcga acgtcgggaa ttatgggatg ctgacacgat
      841 tcctgtctgc gaaataacga tcgtccagcc gacgcggttt caagaagctg tgaggttggt
      901 gatggggtct gcgccacacc tccggttcta tcaagccgat gtggcgctgc cacaactgta
      961 tttctacgac cgcaatctct tccctctttg ccgctgtgac gccgacgtca ccagtgacgc
     1021 aatcattcag gctatccaag ccagcgattc tccgtgggac acggcctatc ggatgccgcc
     1081 cttgtcgatc atagagttga tgttggaagg cgcctcaccc aatccgaacc atgggggagt
     1141 attcaacttg cttgttcgaa tcgaggatac ggagtggcgg gtggaacagg aggatgccgc
     1201 cgaatttatc caaacggtca atggactctt ccaccgctat gatccggacg tgttgctcac
     1261 ggattgggga gattcatata tcctgcctcg gctgatacat ctcgcgcagc tagcgcgcat
     1321 gccgttggcg ctcaaccgag acaccaggca gtcgattgcc gtgcgtgccc ctcgatccta
     1381 tttctcctac ggccatatgg ttgcgcaggc cggatcacaa acgctgtacg gacggctgca
     1441 cctcgaccgc cataattcct tcgcgttggc agaaaccgga gtggctggac tctttgaaca
     1501 ggcacgtgta gcgaaagtac cgatccagca gatggcccgt acgacaaccg gcaccggtat
     1561 tacctcaatg cagttggagc gggcccaccg ttccgggatt ctcattccct accgtaaaca
     1621 acaagcggaa gccttcaaga cggcaggtga gctcttagaa acggatcagg gcgggttcac
     1681 gtatgcgccg ctctccggct atcacgagca tgtgggagaa ctcgactttg cctcgatgta
     1741 tgccgccatc atggtcaagt ttaatgtatc cccggagacg gtgaactgcg cctgctgcga
     1801 gcatgacccc gccgcccagg tgccggagat ttatcatcat acgtgtagga ggttccaggg
     1861 attggtgccg cagacacttg cgcccttgct ggcgaagcga ggtctctata aacagcagct
     1921 caaagatgcc acgaatgaga cgaggaaacg aatcatcgat caacgtcaga cggcgctgaa
     1981 gtggttgctt gtcgtgtcgt tcggctatct cggctacaag aacgcgcgct tcggcagaat
     2041 cgaagcgcac gaatcggtga ctgcctatag ccgggaggtg ctcttacgag ccaaagaaat
     2101 tgcggaatct cgggggtttc gcttgctgca tgcgattgtc gattcgctct ggttgcagaa
     2161 agacgatgca gggagggagg actacgagca gcttgcggcg gcgatcagcg cacagacgga
     2221 gctgccgatt actgtagaag ggctctatcg gtggatcggg tttttgccgt cacgggtgaa
     2281 ccggcacatg ccggtacaca atcaattcgt cgggctgttc gatgatggtc ggatgaaagt
     2341 gcgagggctc gaaatccggc ggtcggacgc gccgcttatc gtcaaacgcg cgcaggccga
     2401 gatcttacag aagctgagtc aggggcgcac gattaccgag ttgaaggtga tgattccgga
     2461 catggtgggg atcgtcgacg agtattgctg ctatcttcgt gaagggcggg cgtccgtcga
     2521 agaagtagcc atcggcaaaa cgttgacgca acctcctgaa cggtatcgcc atgccacacg
     2581 gaccagtatt gcggcaaagg agctccggcg tcgaggggtg tccttgcaac caggtgaaac
     2641 gattcactat gtgatctgcg atagccaggc ggcactgccg gaagatcgtg tacgggctgt
     2701 ggccggttgc gacggcacga ttgcgtatga tgccgaagcc tacatacaac tcgtccacaa
     2761 agccgccctt gcgatgctcg ctccgttagg tgtgacggct aaggagttgg aaaggagaga
     2821 gtaaataagc gtgaagaact attcaccatg cagcccagcc ggcgcatcca tggtgtagtt
     2881 cggatcgtcg atttcccaga cactatgctc cttggagagc ctgctccagc ctcggagata
     2941 cacggctgca agtaccggat cagatgtgat ccctggcttt gtgggaacca cctgacccac
     3001 aaccgtcacc tgcgcccact catggtagtg atccgggatc ttcgctgcat ccacagccac
     3061 ccagtaatag ccggcttcgg cgctatcggg atcgtccgcc aggtggggac gctggttatc
     3121 gtcgagagga cgattcttca tgcggagcca gaggcgtccg tctttgttct cctcgtgcac
     3181 gataaccccg cccaggatca cggtcttgcc ctgatagcgt tccggcgttc gctggagatc
     3241 catcagggtg actcctttct cggcattcca gaggtgccgc cgagggacgg agctacagtt
     3301 gactgcgatg cagaacagcg ccatggcgag cgcccaggca ccgaagcgtc ccacgtacag
     3361 gagccattgt ttttctaacg ggccgtcagg ccgacttttt gtcatggtac attcttgcgc
     3421 agccgaaaag cggatgtcag tgtagggtag cgcgttgtag aagacaagct gcttggtgcc
     3481 tgttcaaggt tgcgcggttt gaaccgattc ggatatggta gccccgcctg tgatgccatg
     3541 aacccaaacc gctcataaag ctgatgtgct cacgcgggat cttcgctgtc ggtatatggc
     3601 ttacgctgat ttcgacggcg ggttgtctgc gtgtcctgtc tcttgattat caagcgacga
     3661 attccatcaa aggacaaggc cccgttcatg tgatgctctt ccggtaccaa tcggcagacg
     3721 agcagcgtgt ccgcccgcat gaagttgaaa ctccgccgag atctgaagcc aaactgtttc
     3781 tttccggcgc gattggaacc gtgttctctc aggcactgca atcagaactt gcacgatccg
     3841 gatatgtgat tgaggaatca agtccgcgca tgatttcagg ggtggtcact cgcttttacc
     3901 tggattggac gaaagaaacg gagagaacgt ttgaactcga ggtgacgttt gaggtgcagt
     3961 cggagggtcg tcagccgttc tcctggagct gttcttcggt gaagagaggg gtcaatatgc
     4021 tcgcagagga cgcgatactg atcaaggctg gcattgcgga ctgtatagag cgattccttg
     4081 tggcgacccg ggtggcgcag gccctttaga aaagtgagga ccaatcagga aacaccgagg
     4141 agacttatcc aatggctatg agtaataaat tgaggactct aggtcgagca agcttggttt
     4201 ggtggcggtg acccggatcg aacgggtgac ccgcggctta tgagtccgct gctctaccaa
     4261 ctgagctaca ccgccacagg aagaggtctc aacaaagtgg gaaggcatga tacaagaaga
     4321 ctcttaaacg tgtccactat gggagtccgg cttttccttg tatccgtgtc ttctacttgg
     4381 tggttggtta ggccggttcg ttgggatcgg ttcttctcgc ctattcaatt tttagacttg
     4441 gcccatgcag atcatccttc ctgagtcctg caagcaggca ttgctcgaac ggtttttgaa
     4501 agccgaagcg atggccctgt gggccgtgca gtccgcgcag gttcagcaga tccaaggtgt
     4561 tccgccgcac gtccttccat tcctccgtcg ccatgaagaa caagaagcgg cacacttgaa
     4621 agagttcgaa cgccgattgg gcgtgcctcc caaggagcgg agccgactcc cgcgtgttcc
     4681 gaggcagtgg tcagccctcg ccgtgcacct cctgggatat gaggccttgg gattggagtt
     4741 tgccaagctg ctggcgcagg ttcagcctga ccttcattca attcttgacg acgagttgat
     4801 tcatgtgggg ttcttcgaga aggaagtcgc acaacttctt ctctcaggtg gatcattggc
     4861 ggatggagca cggctgtcag cgcaagcctg gtggcgccgg ttgcctcgca cgctcgaccg
     4921 gtatctggat ggggcgagtc tggccccata ccggtccgat ctccgccgca cgattcttat
     4981 gacgatcgaa gcgcggttta ctgcggtcgg gctgttagat cctgtgcacg tgcccgcgag
     5041 agtgtgatgg taggtcgttt tccagcctgt tgaaggagcg tttggctgat gagtgatgaa
     5101 aaccacatcg atgagaaaca tgtcctccgg tgtattcttt ttcgacatgg aatcgcagtt
     5161 gagcgggagg agtggagtgg aaaagagagt gatcgacccc tgacggataa ggggaaacgc
     5221 agagtcagac gggtagctgc tggactcaag cgattggacg tacgcccgac gcgtatattg
     5281 acaagtccct taagtcgcgc ggtcgaaaca gcaaaagtgc tgcatgatgc cttggccgtt
     5341 cgatcgccat tacagttggt tgatgaattg ctccctgacg cacctcccga caagatgatt
     5401 gtcttgctca acgatctccc accgggatcc tatgccctct gtgtcggcca tgaacctcac
     5461 cttggcttga ctgcgagtgt gttgttgtcc ggacgacaga cttccgcctt tcctctgaag
     5521 aaagccgggg cgtgcttaat cgaattgtcg cttccggcca agccggggca aggtcggctc
     5581 gtctggtggc tgactcccgg tcaattacgg acgattggaa aggagacgaa caacggagag
     5641 gattaggttg ttggactggg gtgtgcggtg ctgtcggaca acaataccgg gcttcgcaga
     5701 tgattcgaca ggagaggccg gatcttgttc tgcaactctt cctgaatctc ctccggcgat
     5761 ttacgagcat cgatcacttc gaacttgaac tcctcggtca tcttatcgaa ttcctcgatc
     5821 agcagcgact ggtacttctt gaagctgtcg tagaggtctc ccccgagccg caagtccatt
     5881 ccggattccc agaagttcat gcctttcgtt tcgatgacac gcaataccaa cgtctcgacg
     5941 tcaatgcgca agtagcagac gaggtcaggc acgagggcga acccgaacac atctcgaacc
     6001 cacttccgat cgccgctccg gacaaagtcc cgtgcaagcg ccgtatagat gtagcgatcg
     6061 gccaggacaa tgaatcccga acggagggcg ggaatgattt gatgctccaa gcgatcggca
     6121 aagtcggtcg catagagcag ggtgaacgac cagcgatcga gaatgttgcc ttctttcgcc
     6181 atttcgatcg tcttggacat cagattggac cgggtc
//
LOCUS       DATDNW010000098         2944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_77943,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000098 DATDNW010000000
VERSION     DATDNW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77943"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="ribD"
                     /locus_tag="VI359_02570"
     CDS             complement(<1..478)
                     /gene="ribD"
                     /locus_tag="VI359_02570"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY6973167.1"
                     /translation="MTVALRLAAKGRGQTSPNPMVGALVVAKGRIVGRGYHHGPGQPH
                     AEILALQEAGPRAARATLYVTLEPCNHFDKRTPPCVPAVIESGVHRVVVAMVDPNPAV
                     KGRGLAQLRRAGVIVTVGVARREAAHLNRTYIHWVETGRPYVILKAGMTLDGKIATA"
     gene            complement(505..>2944)
                     /gene="ppdK"
                     /locus_tag="VI359_02575"
     CDS             complement(505..>2944)
                     /gene="ppdK"
                     /locus_tag="VI359_02575"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY6973168.1"
                     /translation="EGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAILKHKK
                     TEVGVAHETHLDARHLRELVASFKALVKEETKKDFPDEPLDQLRMAINAVFSSWFGAR
                     AKTYRQLYGIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRNPSTGDKVFFGECLMNAQ
                     GEDVVAGIRTPLPVSALAKNVPEAYKDLEHTYKKLEKHYRDMLDLEFTIQEGKLYMLQ
                     TRVGKRTGIAAVKIAVDMVNEGAISKREAVQRIGPDQLAQYLYPIFDSKEESKANPLG
                     KGLPAGPGAAAGKIALTPDRAVDMKAAGQRVILVRQETSPDDIHGMNASAGFLTARGG
                     MTSHAAVVARQMGKVCVAGCEAVEVVDAQSVRIGAKVFREGDYLSVNGSTGNVYEGDI
                     PVVESEIIQVIQGKLHPSKSDKYQRFSTILSWADDIRKLRVRANADVPDQARIAKGFG
                     AEGIGLCRTEHMFFAEDRIPIMQKMILARTKEEREKYLDQLLPLQKQDFVGLYREMKG
                     YPVTIRLLDPPLHEFLPKREDLMVEIARLELTGNDGALLDEKRRLLARVEELHEFNPM
                     LGLRGCRLGITMPEITRMQARAIMEAACEMAKDGKKIVPEIMIPLVGMVSEMKSQKEL
                     VREAAQESMKKNNVKLSYLVGTMIELPRAAVTADRIAEEAEFFSFGTNDLTQTTFGFS
                     RDDAAKFIDFYKTTNIIDADPFAVLDREGVGSLMKQAIVAGRKTRPTIKLGICGEHGG
                     DPSSVEFCHQLGLDYVSCSPFRVAIARLAAAHAALAESEAKKASQPRSTSGARSSRSA
                     RTAHRRSQVARKKR"
BASE COUNT          574 a          901 c          801 g          668 t
ORIGIN      
        1 gagccgtcgc aatctttcca tccaacgtca ttcccgcttt cagaataacg tagggacgac
       61 ctgtctcaac ccaatggata taggttcgat taaggtgcgc cgcttcccgc cgtgctacac
      121 cgaccgtcac gataaccccg gcacgtcgaa gctgggccaa tccccgcccc ttgacggctg
      181 gattgggatc gaccatggcc acgacgacac ggtgcacacc ggattcgatc actgccggga
      241 cacagggcgg agtccgtttg tcaaaatgat tgcagggttc gagcgtcaca tagagggtcg
      301 ctcgggcggc gcgaggtccg gcttcctgaa gcgcgagaat ttctgcgtgg ggttgcccag
      361 gcccgtgatg atagccgcgg ccgacgatgc gcccctttgc cacgacgagt gcaccgacca
      421 tcggattcgg gctggtctgg ccgcggccct tcgcagccaa acgaagagcc acagtcatga
      481 agcggaggtc ctgtgcggcg gtggtcaccg tttcttgcga gcgacctgtg aacggcggtg
      541 tgcggtccgt gccgatctgc tcgatctggc tccggaggtg gagcgtggct gtgacgcctt
      601 cttggcttcg ctttcggcca acgcagcatg ggcggcagcc aagcgcgcaa tggccacacg
      661 gaacggagaa cagctgacat agtcaagacc gagttgatga cagaattcta cggaactcgg
      721 atcgcctccg tgttcgccgc agatacccag cttgatcgtc ggacgggtct tgcggcctgc
      781 cacgatcgcc tgcttcatca aggagccaac accttcccta tccagcaccg cgaagggatc
      841 cgcgtcgatg atgttggtcg tcttgtaaaa gtcgatgaac ttggccgcgt cgtcgcggga
      901 aaatccgaag gtcgtttgcg taaggtcgtt cgtgccgaac gagaagaact cggcctcttc
      961 tgctatccga tcggccgtga ccgcggcgcg cggcagctcg atcatcgtgc caacgagata
     1021 agacagcttg acgttgtttt tcttcatcga ctcctgagcc gcttcacgca ccaactcctt
     1081 ttgcgatttc atttccgaga ccatcccgac gagcgggatc ataatttccg gcacgatctt
     1141 ctttccgtct ttcgccattt cacaggccgc ttccatgatg gcgcgggctt gcatgcgagt
     1201 gatctccggc atggtaatcc cgagccggca tccccgaagg ccgagcatcg gattgaactc
     1261 gtgcaactct tctactcgtg ccaacaacct gcgtttctca tccagcaacg caccatcgtt
     1321 gcccgtcaat tcgagtcggg caatctccac catgagatct tcgcgcttcg gaagaaattc
     1381 atgaagcggc ggatccagca agcgaattgt gaccggatac cctttcatct ctcgatagag
     1441 gccgacgaaa tcttgctttt ggaggggtaa cagttgatcg agatattttt cgcgctcctc
     1501 ttttgtccgc gccagaatca tcttctgcat gatcgggatg cgatcctcgg caaagaacat
     1561 atgttccgtc cgacagagcc cgatcccttc ggccccgaat cccttggcga tgcgcgcttg
     1621 atccggcacg tccgcattgg ctcgcacgcg caactttcga atgtcgtcgg cccaagatag
     1681 aatcgtcgag aaccgttgat atttgtccga cttgcttgga tgcaatttgc cttggatgac
     1741 ttgaatgatt tcagactcga cgacggggat atcaccttca tacacattgc cggtcgagcc
     1801 attgaccgaa aggtaatccc cttcgcgaaa gactttggcg ccgatacgaa cggactgcgc
     1861 gtcgaccact tccaccgctt cgcaccccgc cacgcagact ttgcccattt ggcgtgcgac
     1921 cacggcggcg tgcgaggtca tgccgccgcg cgcagtcagg aaccccgcgg aggcattcat
     1981 cccgtgaata tcgtccgggc ttgtttcctg ccggaccaga atgacgcgct gacccgctgc
     2041 cttcatgtcc acggcacgat ccggcgtcag cgcgattttc cccgctgcgg cgcctgggcc
     2101 agccggaaga ccttttccca gcggattggc tttggattcc tcctttgaat cgaagatcgg
     2161 atacaaatat tgcgctaatt ggtcggggcc gatccgctgc acggcttcac gtttcgagat
     2221 cgctccctcg ttcaccatat cgactgcgat cttgacggcc gcgatgccgg ttcgtttgcc
     2281 gacccgcgtt tggagcatgt agagcttgcc ttcttgaatc gtaaactcga gatcgagcat
     2341 gtcgcgataa tgtttttcaa gtttcttgta ggtgtgctcg agatctttgt aagcctccgg
     2401 cacgttcttc gcgagcgcgc tcaccggcag cggtgtccga atcccggcga cgacgtcctc
     2461 cccttgcgca ttcatcagac attcaccaaa aaataccttg tcccccgtac tgggatttct
     2521 cgtgaacgcg acgcccgtgc cgctcgtttc tcccatgttg ccgaacacca tcgccacgac
     2581 attgaccgcc gtgccccacg aatcgggaat cccatagagt tgacgatagg ttttggcgcg
     2641 cgcgccgaac caggacgaga agacggcatt gattgccata cgcagctggt caagcggttc
     2701 gtcgggaaaa tccttcttgg tctcttcctt caccaacgct ttgaagctcg ccaccaattc
     2761 acggaggtgg cgtgcatcca aatgcgtttc atgcgctaca ccgacttcgg tcttcttgtg
     2821 tttgaggatc gcctcgaaat gctcgcgcgg gactcccatg acgatactgc cgaacattga
     2881 gacaaagcgg cggtagctgt cctgagcaaa ccgatcattc cgcgtttttg ccgccaatcc
     2941 ttcg
//
LOCUS       DATDNW010000099         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_77947,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000099 DATDNW010000000
VERSION     DATDNW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77947"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2211
                     /gene="secA"
                     /locus_tag="VI359_02580"
     CDS             <1..2211
                     /gene="secA"
                     /locus_tag="VI359_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY6973169.1"
                     /translation="TYEASDKRLQHLRPCTRPEAYRADITYGTNNEYGFDYLRDNLIV
                     SDLSQCVQRELNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINAIIPQLKIER
                     DYTIEEKTKTASLTEDGNVRVEKLLGVDNMYDPANMDLVHHVVKALQAHALYKRDVDY
                     VVKDGEVIIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMY
                     KKLGGMTGTADTEAAEFAKIYNLDVNVVPTNRKMIRLDYADVVYRTEKEKFTAIVEEI
                     KECHERGQPVLVGTISIEKSERLAGLLSRNGVKHNVLNAKQHEREAEIVAQAGRKGAV
                     TIATNMAGRGTDILLGGNPDFMFKQVLYREENLPDDRKLAVYEEIKTDCEKDKQEVVN
                     LGGLHILGTERHESRRIDNQLRGRSGRQGDPGTSRFYLSLEDDLMRIFASERVSQLML
                     KLGMEEGVPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYQHRRAV
                     LGGTNLKDDIYDMMAGVVDSALNVYCPAEQYPEEWDVKGLTEMMQGQFGLDITQGKND
                     GGEALRDLGRDALVEDLKKYVREAYERKEQELGSDLMRFLEKTFMLQVIDHHWKDHLL
                     GMDHLRDGIGLRGYGQKDPLIEYKREGYDLFAAMMERIKSDTLERLFLVQAAKGEAPP
                     PPLTTSAPPPMRLTLNRGDEPAPPQTVQRTADKVGRNDPCPCGSGKKYKKCHGA"
BASE COUNT          619 a          760 c          699 g          452 t
ORIGIN      
        1 acctacgagg cgtccgacaa gcgcctgcag catttgcgcc cctgcaccag accggaagcc
       61 tatcgagcag acatcaccta tggtaccaac aacgagtatg ggttcgatta tttgcgcgac
      121 aatctaatcg tgagcgacct gagccagtgc gtgcaacgcg agctgaattt cgccatcgtg
      181 gacgaagtcg acagtattct gatcgacgag gctcggactc cattgattat ctccggcccg
      241 accgaccaaa cgaccgacct ctactaccgg atcaatgcca tcatcccgca gctcaagatc
      301 gagcgtgact atacgatcga agagaaaacc aagaccgctt cgctcacgga ggacgggaac
      361 gttcgcgtcg aaaaactgct cggcgtcgac aacatgtacg atccggccaa catggatctc
      421 gtccatcatg tcgtcaaggc gcttcaagcc cacgcgctct acaaacgcga cgtggactat
      481 gtcgtcaaag acggcgaggt catcatcgtc gacgagttca cgggccggct catgccgggc
      541 cgccgctgga gcgacggcct ccaccaggcc gtcgaagcga aggagggcgt gaagatcgcc
      601 aacgaaaatc aaacgctggc ctccgtgacc ttccagaatt atttccgtat gtacaagaag
      661 ctcggtggta tgaccgggac ggcggacacc gaagctgcgg agttcgccaa gatctacaat
      721 ctcgacgtga acgtggtccc gaccaatcgc aagatgattc ggctggacta cgccgacgtc
      781 gtgtatcgga cggaaaaaga aaagttcacc gccatcgtcg aagagatcaa agaatgccac
      841 gagcgcggcc agccggtgct ggtcggcacc atttcgatcg aaaagtccga gcgactggcc
      901 ggcttgttga gccggaatgg cgtcaagcac aacgtactca acgccaagca gcacgaacgc
      961 gaagccgaaa tcgtcgcgca agccggacgc aaaggcgccg tcaccatcgc cacgaacatg
     1021 gccggccgtg gcaccgacat tctcctgggc ggcaatcccg acttcatgtt caaacaggtg
     1081 ttgtaccggg aagagaatct gccggacgac cgcaagctcg cggtctacga ggagatcaaa
     1141 actgattgtg aaaaagataa acaagaagtc gtcaatctcg gcgggttgca catcctcggc
     1201 accgagcggc atgagagccg ccggatcgac aatcagttgc gcggccgttc cggccgccag
     1261 ggcgatccgg gcacctcgcg tttctatctg tcgctcgaag acgacttgat gcgcatcttt
     1321 gcttcagagc gtgtctcgca gctcatgctc aagctcggca tggaagaagg cgtgccgatc
     1381 gagcatggca tggtgacccg tgccatcgcc aacgcgcaga agaaggtcga agcccacaat
     1441 ttcgaaattc gcaaacagct gctcgaatat gacgatgtca tgaacaagca gcgcgaggtc
     1501 atctaccagc atcgccgagc cgtcttgggc gggaccaatc tcaaggacga catctacgac
     1561 atgatggcag gggtcgtcga ctcagccctc aacgtgtatt gtccggccga acaatacccg
     1621 gaagaatggg atgtgaaggg cctcaccgaa atgatgcaag gccagttcgg cctcgacatc
     1681 acacaaggga aaaacgacgg gggcgaagcg ctccgcgatc tcggtcgaga tgctctcgtg
     1741 gaagatctca aaaagtatgt gcgtgaggcc tacgagcgga aggaacagga actcggctcg
     1801 gacctcatgc gattcctcga aaagacgttc atgctccaag tcatcgatca tcattggaag
     1861 gaccatctgc tggggatgga tcacctgcgc gatgggatcg ggctgcgcgg ctatggtcag
     1921 aaagatccac tcatcgagta caagcgggaa ggctacgacc tgttcgccgc catgatggaa
     1981 cgcatcaagt ccgataccct cgagcgtctc ttcctcgtcc aggccgccaa aggcgaggcc
     2041 ccgcctcctc cgctaacaac atccgcaccg ccgccgatgc gactcactct caaccgcggc
     2101 gacgaaccgg ctccacctca aaccgtgcaa cgcaccgccg acaaagtcgg ccgcaacgac
     2161 ccctgcccct gcgggagcgg gaagaaatac aaaaagtgcc acggggcctg agtaccaacc
     2221 tcatctcgcg attcggcgca aacgatcaca tcgatttttc agcaaactct acgccgctcc
     2281 catctcttat cgagagacaa ttcccgctga tttcaccttg acttagcagc aatcccgagg
     2341 ctaatctacc gctcttttag cctccagtag cgatgggtgg agtgcaccca ttagctattg
     2401 gtgtctgcgc atgagtatag tcagcggacg aagggagttg tgtgtgacga ccggccaccc
     2461 tgaactcgtc gccgccattg tctctgagat cgcgcgatcc ggcccgatcc cgtttgtccg
     2521 gttcatggaa
//
LOCUS       DATDNW010000100         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_351169, whole genome shotgun sequence.
ACCESSION   DATDNW010000100 DATDNW010000000
VERSION     DATDNW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351169"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VI359_02585"
     CDS             complement(<1..591)
                     /locus_tag="VI359_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY6973170.1"
                     /translation="MMKFTIWEALFILSLVGNSVAASEGAGADTGRDLSPREIYEQTA
                     PAVVMVMGHSGGGTSGSGGTGSIIQADGTILTNAHVVIEERTGKPYPRLSVFLKPARV
                     TGDSKMDLSRMFRARVLAFSQPLDLALLKLEGIREPLPVVSLSDSARSRIGDHVVAIG
                     HPEQGGLWTLTTGVISAEVDNFNGVKGKNVFQTETGL"
     gene            complement(727..1845)
                     /locus_tag="VI359_02590"
     CDS             complement(727..1845)
                     /locus_tag="VI359_02590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6973171.1"
                     /translation="MNVPLLDLKAQYRAIRTEVLEAMATVCDEQGFVLGPRVSAFEGA
                     VAQYVGSAHAIGCASGSDALLLALMAMGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     VDIQPDTFNIDPRQVERALNPKTKVIIPVHLFGQCADMAGLNQIARQKRVRVIEDACQ
                     AIGAAQQGRRAGILGDTGCFSFFPTKNLGGFGDGGIITTNHDGLAESMAMLRVHGSRV
                     RYLHEAVGINSRLDALQAAVLHVKLKYLDQWTEGRRRNADRYRQLFDDVKLQDRVILP
                     PTASGNFHVYNQYTIRVQKRDELRTYLKEHGVGTEVYYPLPMHLQNCYRDLGHRKGAF
                     PVSERAAEEVLSLPIYAELTGEQQGYVVEKIAEFFQRQ"
     gene            complement(1869..>2294)
                     /locus_tag="VI359_02595"
     CDS             complement(1869..>2294)
                     /locus_tag="VI359_02595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6973172.1"
                     /translation="QYGLKNRVHLAGWRRDVSTVMKAFDAFLLTSHWEGLPRVLLEAR
                     AAGIPVVATRVGGVEEVIVEGRHGWLSEVGDIHTLSEQLSHVLRGREDLSPDGPGNSE
                     ALPKEFHRDEMVKQYESLYDRLLIDQQGKNRPSTAWSAS"
BASE COUNT          502 a          718 c          581 g          493 t
ORIGIN      
        1 caatcccgtc tctgtctgaa atacattctt gcccttcacc ccgttgaagt tgtcaacctc
       61 tgcgctgatg acgccagtgg tcaaggtcca gagtcctcct tgctcgggat gcccgatggc
      121 caccacatgg tccccgatcc tactccgcgc cgagtcgctt agactcacca ccggaagcgg
      181 ctccctgatg ccttcaagtt tgagcaacgc caaatccaat ggctgagaaa atgccaggac
      241 cctcgcacga aacatgcgag acaggtccat ttttgaatca cccgtcaccc gcgccggctt
      301 taaaaacacc gacaggcgcg gatagggttt ccccgttcgt tcctcgatga caacatgggc
      361 atttgtgagg attgttccgt cagcctgaat gatcgacccg gttcccccgc tcccgcttgt
      421 gccgccacca gaatgtccca tcaccatcac gacggcagga gccgtttgct catagatctc
      481 acgtggcgag aggtctctcc cagtatctgc accggcccct tctgaagcgg ccactgaatt
      541 tcctacgaga gacaggatga acaaggcttc ccagatcgtg aacttcatca tggcctcctt
      601 ggggatggtg cagaccggtc cccctttcac acaggctcat tatacggcac attatataac
      661 gcagggggag taccagcgtt gacaaaacag gcttcccgca ggtcaggagc cgagtcggct
      721 ccgagactat tgacgctgaa agaattcggc gatcttctcg accacatatc cctgttgctc
      781 gccggttagt tcagcgtaaa ttgggagaga cagcacctct tcggccgccc gttccgaaac
      841 cggaaacgcc ccctttcgat gacccaggtc acgatagcaa ttctgcagat gcatggggag
      901 agggtaatac acttcggttc cgaccccatg ctctttcagg tatgttcgca gctcatcccg
      961 cttctgcacc ctgatggtgt attggttgta gacgtgaaag ttgcccgacg ccgtcggcgg
     1021 gaggatcacc cgatcctgaa gcttcacatc gtcgaagagt tgccggtacc gatcagcgtt
     1081 ccgacgtcgg ccttcggtcc attgatcaag gtacttcagc ttcacatgga ggacagctgc
     1141 ctgcagcgca tcaagccggc tgttgattcc cactgcctcg tgcagataac gaacgcgact
     1201 gccgtggacc cgaagcatcg ccatcgactc cgcgagaccg tcatgattcg tggtgatgat
     1261 acccccatca ccaaacccgc cgaggttctt ggtggggaaa aagctgaaac agcctgtgtc
     1321 cccgaggatg ccggctcgcc tcccctgttg agcagcaccg atcgcctggc aggcgtcttc
     1381 aatgacgcgg acacgtttct gccttgcaat ctggttcaac cccgccatgt cggcacattg
     1441 cccgaacagg tggacaggga tgatgacttt ggttttcgga ttcagcgctc gttcgacctg
     1501 tcgagggtcg atgttaaacg tgtccggctg aatgtccacg aagaccggtt tggcccccaa
     1561 tcgggaaatg gcgcccgccg tggcaaagaa cgtgaacggc acagtgacga cttcatcccc
     1621 cgccttgacg cccatcgcca tgagcgccaa caacaatgca tcactgccgg acgcgcaacc
     1681 gattgcatgc gcactcccca catactgcgc gacagcccct tcgaaggccg agacacgcgg
     1741 acccaacaca aatccctgtt cgtcacagac cgtcgccatg gcttcaagca cttccgtacg
     1801 aatagcgcga tactgggctt tcagatccaa taacggaacg ttcacaacag gctcctcagg
     1861 agtaaaggtt aggatgccga ccaggctgtc gaggggcggt tcttcccttg ctgatcgatc
     1921 aggagcctat catacagaga ttcatactgt ttgaccattt catctcgatg gaattctttc
     1981 ggcaaggctt ccgagtttcc cgggccatct ggcgagagat cttctcgtcc ccgcaaaacg
     2041 tgactgagtt gttccgagag cgtatgaata tcaccgacct cgctcaacca cccatgacga
     2101 ccttccacaa tcacttcttc gactccaccc actctggtcg ccacgacggg aataccggct
     2161 gctctggctt cgagcaacac tcgtggtagc ccctcccaat gggacgtgag aagaaacgcg
     2221 tcgaatgctt tcataacggt cgagacatcc cgtctccaac cggcgagatg gactcgattc
     2281 ttgagtccat actg
//
LOCUS       DATDNW010000101         2643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_424738, whole genome shotgun sequence.
ACCESSION   DATDNW010000101 DATDNW010000000
VERSION     DATDNW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424738"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..998
                     /gene="lpxI"
                     /locus_tag="VI359_02600"
     CDS             147..998
                     /gene="lpxI"
                     /locus_tag="VI359_02600"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY6973173.1"
                     /translation="MSQPRHANSGQRIGLIAGNGRFPIIFATNAKQLGYHVSAVAHEG
                     ETEPELANHVDRIQWIKIGQLNKLIQAFKEDNISQAVMLGGIKKTHVFTTVRPDFRAL
                     AMAAKLALWKDDDILREFAAELEREGIMIRESTFGLEGILVEEGTLTRRSPTKKEWDD
                     IRYGWEVAHEIGRLDIGQCVVIKDRVVVAVEAVEGTDAAIKRGGELAQDGAVVVKRSK
                     PQQDLRFDLPAVGPRTIEVMQSVKASVLALEAGKTVLLDREVMLEKARQADITVVGIA
                     ALEASPA"
     gene            1009..2010
                     /locus_tag="VI359_02605"
     CDS             1009..2010
                     /locus_tag="VI359_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6973174.1"
                     /translation="MTPLRAGVVGVGHLGQHHARLYASLPGSQLIGVVDQSPERGQVI
                     AERHGVRLFRNVEELLPQVDLVSIAVPTSGHYEVTRRCLQAGKHVLVEKPLAVMPEEA
                     RELVDLAKARDCRLQVGHSERFNPVMRVMRPHIGRPVFIECHRLSSFSERGTDVDVVL
                     DLMIHDLDLVLSFDPGPVEEIRAAGVAVLSASIDIANARIQFRSGCVANFTASRVSTN
                     KMRRLRLFQPDQYLSLDFQTRQGMICRRQTRPGERPTVVIEQFQGEDEEPLKLQLESF
                     LDVVRTGKKPVISGEDGAAAVAVAHDVLQAIATFASRHGGPDRASGSAGAANPSGRR"
     gene            2048..>2643
                     /locus_tag="VI359_02610"
     CDS             2048..>2643
                     /locus_tag="VI359_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY6973175.1"
                     /translation="MPRILIVTGEASGDLHGANLAKAIMALDSTAELVGIGGAAMRAA
                     GVALVPGVPQLDLMGLIGISALGAVLRRIRAIRTVLKTQPWDLVVLIDNPGLNFHFAR
                     VAKSAGHRVLYYIAPQIWAWRPRRIKWIQRRVDHVVVILPFEPDLYRRAGVRCTFVGH
                     PLLDAVAPRYDRSALRTKFGLHEGGPVVGLLPGSRAGEV"
BASE COUNT          511 a          732 c          886 g          514 t
ORIGIN      
        1 cggacggcgg aagccgcaaa attggcgaaa acggagttcg tcgatcaacc cgacgtcatg
       61 acggtggtgg cattcatgga aggtaccaag cgcggaatct gccggtccgt cgagcgtggt
      121 cgggaagatg aggaggagta agccgggtgt ctcaaccgcg tcacgcgaat agcggacaac
      181 gcatcgggct catcgccggc aacggccggt ttcccatcat cttcgccacc aacgccaagc
      241 aactcggcta tcatgtgtcg gcggttgccc acgaaggcga aacagagccg gagttggcca
      301 accatgtcga tcggattcag tggatcaaga tcggccagct caacaagctc attcaagcct
      361 tcaaggagga caacatcagc caggcggtga tgttgggcgg tatcaaaaag acgcacgtct
      421 ttacgacggt gcgaccggac tttcgggcgc tggcgatggc agccaagttg gcactgtgga
      481 aagacgatga cattcttcgc gaattcgccg ccgagctgga acgggagggg atcatgatcc
      541 gcgagtccac gttcggtctc gaggggatct tggtcgaaga aggaaccctg acccggagaa
      601 gccccacgaa gaaagaatgg gacgacattc gctacgggtg ggaggttgct cacgagatcg
      661 gacgcctcga catcggccaa tgtgtggtca tcaaggaccg tgtcgtggta gcggttgagg
      721 cagtggaagg caccgatgcg gccatcaagc gtgggggaga attggcacag gacggggcgg
      781 tggtcgtcaa gcgctcgaag ccgcaacagg atttacgctt tgaccttccc gccgtcgggc
      841 cgcgcacgat cgaggtgatg cagtcggtca aggcctcggt gctggcgctg gaggcgggca
      901 agaccgtgct gttggatcgg gaagtcatgc tcgagaaagc gcgccaggcc gacatcaccg
      961 tcgttgggat cgccgcgctc gaagcgagtc ctgcatgagc gacagaacat gacaccgttg
     1021 cgagcgggcg tcgtcggggt cgggcatctg ggccagcatc atgcgcgtct ctacgcgtcg
     1081 cttcccgggt cccaactgat cggcgtggtc gatcagtcgc ctgagcgggg gcaggtgatc
     1141 gctgagcgac acggggtgcg tctgttccgg aacgtcgagg aactgttgcc tcaggttgac
     1201 ctcgtcagca tcgcggttcc cacctcgggg cattatgaag tgacgcgccg gtgtttgcag
     1261 gccgggaaac acgtgctggt cgagaagccg ctcgccgtga tgccggaaga agcccgcgaa
     1321 ctcgtcgacc tcgcaaaggc gcgcgactgt cgcctgcaag tcgggcatag tgaacgattc
     1381 aatcccgtga tgcgggtcat gcggccgcat attgggcggc cggtcttcat cgaatgccat
     1441 cgtctcagct cgttcagcga gcggggaacg gatgtcgacg tggtgctgga tttgatgatt
     1501 cacgacctgg atctcgtcct gtcgttcgat cccggaccgg tcgaagagat tcgcgcggcc
     1561 ggtgtggcag tgttgtcggc ttcgatcgac atcgcgaatg cgcgcattca gtttcggagc
     1621 ggctgtgtcg ccaattttac cgccagccgt gtctcgacca ataaaatgcg ccgtctgcga
     1681 ctgtttcagc ccgatcaata tctctcgctc gactttcaga ctcgtcaggg gatgatttgc
     1741 aggcgccaga cgaggcctgg cgagcgtccg accgtggtca tcgaacaatt tcaaggcgag
     1801 gacgaagaac cgttgaaact ccagctcgaa tcgttcctcg acgtcgttcg cactggaaag
     1861 aagccggtga tatccggcga ggacggagcg gctgccgtcg cggtggcgca cgatgtgttg
     1921 caggcgatcg cgacctttgc ctcgcgtcat ggagggccgg accgggcatc cggttcggca
     1981 ggcgccgcca acccatctgg tcgaagatga attcgtcgga gccgatcacg gcaaggccgt
     2041 ttcccccatg ccacgaatcc tgattgtgac gggcgaagcc agcggagatc ttcacggagc
     2101 caatttggcg aaagcgatta tggcgctcga ttcgacggcg gaactcgtcg gtatcggggg
     2161 ggctgcaatg cgggctgccg gtgtggcctt ggttccgggg gttccccagc tggatttgat
     2221 gggactgatc gggatttcgg cccttggcgc ggtcctccgg cgcattcgcg cgatcaggac
     2281 ggtactcaag acgcaaccat gggatctcgt ggtgttgatc gacaacccgg ggctcaattt
     2341 ccacttcgca cgggtggcca agagtgccgg tcaccgggtg ctctactata ttgcgcccca
     2401 aatatgggcc tggcggcctc gtcggatcaa gtggattcag cggcgtgtcg atcatgtggt
     2461 ggtcattctc ccatttgaac cagacctcta tcgccgggcg ggagtccgct gtaccttcgt
     2521 cggccacccg ctgcttgatg cggtggcgcc acggtatgat cgaagcgcgc tcaggacaaa
     2581 attcgggttg catgagggcg gccctgtggt cggattactt ccgggaagtc gggctggtga
     2641 ggt
//
LOCUS       DATDNW010000102         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1035432, whole genome shotgun sequence.
ACCESSION   DATDNW010000102 DATDNW010000000
VERSION     DATDNW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035432"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VI359_02615"
     CDS             <1..1075
                     /locus_tag="VI359_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973176.1"
                     /translation="YRARVRLGVSVAAALLARLFGHPARYDCVLVGVCVTVLRLCFVT
                     VEGMAAEWSAITEAKVLYTDNVFEFSGARRLRLSEDPSQPTIVPLSRPSDTVWEPSID
                     VRRTSSSNLGQTEVSFKAHGFIFANNPIFNHGDYRIQVNQQVAPDTFVLLRYRYVPNL
                     FLGPNTERQTGNLLPTEERVTSHTWRAQFERKLNDRWTATLVGRYGLRFYNNAFSERD
                     TWFYTIGPRVQFQATSWATITLDYLYERGFADGRNEPQFKDDVSYWQDFVSFGTLFQL
                     TDPLSLELIYVYRQKQFTTDIAGDPLHGAVDNLHQGTAEVHYDVSPAARVTLGYQRTQ
                     RSSNVGSRDFFNDNASLGVEYRF"
     gene            complement(1284..1784)
                     /locus_tag="VI359_02620"
     CDS             complement(1284..1784)
                     /locus_tag="VI359_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY6973177.1"
                     /translation="MKKIHLIANKSFLILWSALVVLWTLIAHAGALTGQVGSVLDGDI
                     LEVLHQGKAERVRLNGIDCPEKDQAYGERAKQAISDLVVGKEVMLNTYGLDRNGRTLA
                     DVLLPDGTNVNYKLVKEGWCWWYRKYAPGNRKLEGLENEARVAKKGLWAGPHPVPPWE
                     WRKKRK"
     gene            complement(1781..2104)
                     /locus_tag="VI359_02625"
     CDS             complement(1781..2104)
                     /locus_tag="VI359_02625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973178.1"
                     /translation="MDLRRHQRFPVHFHSVLSGPKLSESAGTIVNLSEGGCCVETDSQ
                     VYTGIQLALRLHVPGDETPIRIEQAAVRWTRGHELGVGFITITPPHKERLDQFLERLK
                     REQGA"
     gene            2317..2874
                     /locus_tag="VI359_02630"
     CDS             2317..2874
                     /locus_tag="VI359_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973179.1"
                     /translation="MNYRAYVTLRVPSLVLIVGVITIVLASTSSLAEQPTEASERESL
                     RGVTGVEVRVDPLDVEVEQLGLQTSTLQKEIKQRLQKAGVNVLTERERLKTPTAAMLV
                     IRVDTLHDRIGRYFYSIDLLLTQRVHLEGQASAELSAVTWMKLGSIGTVADDNIKHIE
                     TQILQKADQFIKEYLAMNPDRKVKS"
     gene            3051..3668
                     /locus_tag="VI359_02635"
     CDS             3051..3668
                     /locus_tag="VI359_02635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY6973180.1"
                     /translation="METGFDWIGRYRYGAIFTLLMLGIVGLPVSDETLLIFVGYLSFK
                     GTLQLEPAIATAFLGSASGITLSYMLGRFVGLPALIRFGYLFHLRPDHLVRSQQWVER
                     WGKYSLLVAYFIPGFRHLGALILGAALLPPTVFARFAYTGALIWSASFIGIGYIGGEE
                     WNQVSPLLHRTVIVVTLLVLFAFAIGVTWFLIRRTFRTHSRAGEG"
BASE COUNT          823 a         1095 c         1068 g          948 t
ORIGIN      
        1 gtaccgtgca agagtacgcc tcggcgtttc tgtcgctgcg gccttgctgg cccgcctctt
       61 tgggcatccc gccaggtacg actgtgtgct cgtgggagtc tgtgttactg tgcttcggct
      121 ttgcttcgta accgtcgaag gaatggctgc ggaatggtcg gcgatcactg aagcgaaagt
      181 tctctatacc gacaacgtgt tcgagttttc gggcgctcgc cgcttgaggc tgagcgagga
      241 tcccagccag ccaaccatcg ttccgcttag ccgaccatcc gatacggtct gggaaccgtc
      301 catcgatgtg cggagaacct cgtcctcgaa tctcggccag accgaagtgt cattcaaggc
      361 gcacggattc atttttgcga acaatcctat tttcaatcat ggggactacc ggatccaggt
      421 gaatcaacag gtggcgccgg acacctttgt tctcctccgc tacagatatg tccccaattt
      481 gtttctcggt cccaacacgg aacgccagac gggtaatctc ctgccgacgg aggagcgtgt
      541 gacctctcat acgtggcgag cgcagtttga gcggaaatta aacgacagat ggactgcgac
      601 tctcgtcggc cgctatgggc tgcggttcta caacaacgcc ttttcggaac gggatacctg
      661 gttttacacg atcggcccgc gcgtgcagtt tcaggcgaca tcgtgggcca cgatcacgct
      721 ggactacctg tatgaacggg gatttgccga tgggcgaaac gaaccgcaat tcaaggacga
      781 cgtgtcgtac tggcaagatt tcgtctcatt tggaaccctg tttcaattga ccgacccgct
      841 ttcgctggag ttgatctatg tctaccggca gaagcagttc accaccgaca tcgcgggcga
      901 tccactgcat ggtgctgtag acaatctcca tcaagggacg gcagaagttc attatgacgt
      961 atctcccgct gcccgagtca cgctggggta ccagcgcacg caacgctcgt ccaacgtggg
     1021 gtcgcgcgat ttcttcaacg acaacgcctc gctcggtgtg gaatatcgat tttgaaatcg
     1081 gttcgccgag aaagcgccaa tacaggagag gcgttctaga aatgataggc gatgccgcct
     1141 agtgcgctga acggactgta gttgccgctc attccgaacg tgttataaag gttcgtaatc
     1201 tgcgcatggc tgtatttacc ctcgagaaag aatctccatt cctcaaagtg gaatgaaatt
     1261 ggcaggccgg cctggttgtc ggcttacttc cttttcttcc gccattccca cggaggtaca
     1321 gggtgtggtc ctgcccataa acctttcttt gccactcgcg cttcattctc gagcccctca
     1381 agttttctgt tccccggcgc atatttccga taccaccaac accatccttc tttgaccagt
     1441 ttatagttga cgttggtgcc atccggtagg agtacatcgg caagggtccg cccgttcctg
     1501 tcgaggccat acgtattgag cataacttcc ttcccgacca ccaagtcgga gatggcttgc
     1561 ttcgcccgtt cgccgtatgc ctgatctttc tcgggacagt cgatgccgtt gagtcgtacc
     1621 cgttctgctt tgccttggtg gaggacttcg aggatatctc cgtcgagcac gctgccgacc
     1681 tgcccagtca acgcacctgc gtgagcgatg agcgtccata agacaacaag ggcactccac
     1741 aggatcaaga agctcttgtt cgcgatcaag tgaatctttt tcaagcccct tgttctcgct
     1801 ttaatcgctc aaggaattgg tccaatcgct ccttgtgcgg cggtgtgatg gtgatgaatc
     1861 ccacgcctaa ttcgtggcca cgggtccaac gcaccgctgc ctgttcgatg cggattggag
     1921 tttcgtcgcc gggcacgtgc aggcgcaggg ccagctgaat gcctgtgtat acctggctgt
     1981 cggtctcgac acagcagcca ccctccgaca aatttacgat cgtcccggcc gattcactga
     2041 gctttggacc agagagcaca ctgtgaaagt gtacaggaaa gcgctggtgg cggcggaggt
     2101 ccataagagc cttcctccca atcagtgtgt gaggtgcgct gtgactcccc cttcggcagt
     2161 gttccctttc gattccgatg tctcgcaaat atctgacaaa acaaccgaca ttgcctgctg
     2221 ccgacgcaag gctaggctgt ctgtggaaaa gtgtcaaggc tgacagatgc gatcgaaggg
     2281 gcgtaggata ttacaggtcg ggaaggaggc attgccatga attatcgtgc atacgtcacg
     2341 ctccgtgttc cttctctggt tctgattgtg ggggtcatca cgatcgttct cgcctcgacc
     2401 tccagtcttg ccgagcaacc caccgaggct tctgagcgtg agtcgttgag gggggtgacg
     2461 ggcgtggagg ttcgcgtcga tcctttggat gttgaggttg aacagctggg gcttcagaca
     2521 agcaccctcc aaaaagaaat caagcagcgc cttcagaagg ctggtgtgaa tgtgttgacc
     2581 gagcgcgagc gactcaagac tccgactgcg gcaatgctgg tgattcgagt ggatacgctc
     2641 catgatcgca tcggccgata tttttactct atcgacttgt tgttgacaca gcgcgttcac
     2701 ctggaaggcc aagcgagcgc agaactctcc gctgtgacct ggatgaaact tgggtcgatc
     2761 ggcaccgtcg ccgatgacaa tatcaagcat atcgaaactc agatcctcca aaaggctgat
     2821 cagttcatca aagagtatct cgctatgaat cccgatcgga aagtcaaaag ttagcggctc
     2881 gcccagtggc gcaacgttct acagaatctc ccattcaaga aacctcaacc tgatcaattc
     2941 gctcacaatt acgccaaccc cgcaaaggta agatcgacga tgacgttcct tgatgttttg
     3001 ttggatggct ctggccttgg gatgagtcgg gggctaagct agcacctgtg atggaaactg
     3061 gcttcgattg gatcggccgc tatcggtatg gagcgatctt cactctgctc atgctgggta
     3121 tcgtgggact cccagtttca gacgagacgt tactcatctt cgtcgggtat ctcagcttta
     3181 aaggaacgct ccagcttgag ccggcaatcg ccactgcctt tttgggaagt gccagcggta
     3241 ttaccctcag ttacatgctc gggcggtttg tcgggcttcc cgccctcatc agattcggct
     3301 acctgtttca ccttcgacca gaccacctgg tccgatcgca gcaatgggtc gaacgctggg
     3361 gaaagtattc ccttctcgtg gcttatttca tcccggggtt ccgccatctt ggcgcactga
     3421 tattaggggc tgcactcctt cctccaacgg tattcgccag gtttgcctat actggagcgt
     3481 tgatctggtc ggcgagcttt atcggaatcg gatacatcgg tggagaagag tggaaccagg
     3541 tttccccgct cctgcatcga acagtgattg tcgtgacact gctggttctt ttcgcattcg
     3601 cgatcggagt gacatggttc ctcatacgac gaaccttcag gacgcactct cgtgccggtg
     3661 agggatgagg atgtgagtca ctgccgctac gtcggcagca agtgttctat aacggcttga
     3721 ttgtttggtg ggccgtgtag ggcgaaccta cggcccgctg gttaagcgat gcaagtctga
     3781 cccgcagaag ccgccaaaac cgcatgaata gcggacttgt gatgtgacaa agtagtccca
     3841 aacatgacca tctgaatgcg cccatgcagc ccccgcgcaa ccagcatctg acctcccccg
     3901 taccctataa aaagtccaat tccaaacccg cggg
//
LOCUS       DATDNW010000103         5030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_490412, whole genome shotgun sequence.
ACCESSION   DATDNW010000103 DATDNW010000000
VERSION     DATDNW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490412"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="VI359_02640"
     CDS             complement(<1..2252)
                     /locus_tag="VI359_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY6973181.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRESDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQLAQEGQLDPVIGRADEIERVLQILSRRSKNNPVLIGESGVGKTAIVEGL
                     AQRIVMSEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     YVQPPSIDETVRIIQGLRDRYEEHHGVEITEDAIVEAVKLSDRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPPELKAMEQELKKVSRDKEMAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKQKPTIGKEDVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVV
                     GQNEAISAVARAIRRSRAGLKEARKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTERVRRRPYSVVLFDEIEKAHPDVF
                     NILLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKLIQKGVSLGFQSTEVEQDRRKK
                     EEVLGELRRSFSPEFLNRIDEIVVFHQLDKEHLNSILDILLRELNLRLLEKGVEIEID
                     DEVKQWLIKEG"
     gene            2510..3370
                     /locus_tag="VI359_02645"
     CDS             2510..3370
                     /locus_tag="VI359_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY6973182.1"
                     /translation="MKSKSIGILTKPKFPEVKTTLHAVVTWLRARNIEVLLDTTSATL
                     LGEPGGIQKTQLASKAGVLLVLGGDGTMLNAARLAAERGIPILGVNMGGLGFLTEVRL
                     EHLYPSLERVFANEFVLDERLMLRTHVHRHGETVAQGVVLNDVVISKGTLARMIELKV
                     SIQGQFVTNLRGDGLIVSTPTGSTAYSLSAGGPIIDPAVQSLILTPICPHTLTHRPLI
                     VPSNAEIDVTLTSKDDGAMTTLDGQVGIAMTQGDTVAIQASEHRTRLIRFPETHYYDV
                     LREKLNWGDR"
     gene            complement(3391..4761)
                     /locus_tag="VI359_02650"
     CDS             complement(3391..4761)
                     /locus_tag="VI359_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6973183.1"
                     /translation="MSAIGSKGETHVVVVVGAGPAGMAVAHKMAEAGHEVIILNRDIK
                     FGGLAEYGIFPSKLKLRGGLKKTYWSILEAPNVHYFGNVSVGKSKDLTVEDLRELGAS
                     ALVFSTGAQGTKTIGVEGDSARGVFHAKDVVYHFNRLPGFGDRPFEMGNHVAVIGAGD
                     VMVDIAHWLIRYKKVDRVTAVVRRGPAERKYNPKEIRAVCSNMDVDGITKEFARIKDR
                     LAAVGQKAEEILKSVLDEFTKCEPKVSDTKMDFRFLASPKRILVDGNNRVRGLEMEEN
                     KLEPKGEDTAAVGLKQYYEFPCDSVVFAVGDKVDETVGLPYKNGVFVTNPNKTTNDPD
                     DALFQAYDEVSGKVVDGVFLAGWARKASEGLVGVAKRDGEWCAEVVSRYLKTKAPHSR
                     ASAEGILSKLQTTLKNRNSHPVNVKGLRVLEAAEQAHKGPADCIGAFKHVSNREMLGL
                     IEKALS"
BASE COUNT         1075 a         1457 c         1256 g         1242 t
ORIGIN      
        1 ccttctttaa tgagccactg cttgacttca tcgtcaattt caatctccac gcctttttcc
       61 agcaggcgca ggttcagctc tctgagcaag atgtcgagaa tgctgttgag gtgttccttg
      121 tcgagctggt ggaacacgac gatctcgtcg atccggttca aaaattctgg actgaacgag
      181 cggcgcaatt cgcccagcac ttcttccttc ttacggcgat cttgttcaac ttccgtactc
      241 tggaacccaa gggacacgcc tttctggatc agcttggtcc caatgtttga ggtcatgatg
      301 accacagtat tcttaaagtc aaccttgcgt cctaagctgt ccgtcagaac cccgtcgtcc
      361 agaacttgca acaggatgtt gaacacgtct gggtgtgctt tttcaatttc gtcgaagagg
      421 acgaccgaat agggacgacg acgcactcgt tccgtcaact gaccgccttc ctcataccca
      481 acatagccag ggggggctcc gaagagccgt gaactggtga acttctcctg atattcggac
      541 atgtcgatcc gaatcaaggc atcctcgcta ttgaatagga attcggccaa cgttctcgcc
      601 aactcggtct ttcccacgcc ggttgggccg aggaaaataa aggaaccgat cggctttcgt
      661 gcctccttca gaccggctcg cgatcgccga atggcacgcg ccactgcgga gatggcctca
      721 ttttgaccaa ccacgcgttt atggagaaat tcctccatgc ggagcagctt gttcgattct
      781 tcttcctcga gcttgaagag cgggatgccg gtcattttag aaacgacata ggccacgtct
      841 tccttgccga tcgtcggctt ttgcttttcc tggttctttt tccactcgcg tttcgattcg
      901 tccagaagtt tgcgcaagcg ctcttcttct tcccggtggc gaacggcttc ttcgaagttc
      961 tgcatcgaga tcgccatctc tttgtcgcgc gagactttct tcagttcctg ttccattgcc
     1021 ttgagctcag gcggcagcgc ataggtttga agcttggctc gcgagccagt ctcatcgatc
     1081 aagtcgatgg ctttatcggg caagaaccga tcggtgatgt agcggtcgga taacttgacc
     1141 gcttcgacga tggcgtcttc cgtaatttcg acaccatgat gttcttcata ccgatcccgc
     1201 aggccttgga taatccgtac tgtttcatcg atgcttggcg gctgcacata aatcggctgg
     1261 aatcgacgct tgagtgcacc gtctttctca atgtgttttc gatattcgtc aagtgtagta
     1321 gctccgatgc actggatttc gccgcgagac aaagccggct tgagcatgtt ggatgcatcg
     1381 atcgaccctt ccgccgcacc agcgcccact aaagtatgaa gctcgtcgat aaagatgatg
     1441 atattgccgg cctgcacaat ttccttcatc acgaccttca gacgttcttc gaattgtcca
     1501 cgatactttg tgccagcgac caaggaacca agatcgaggg cgatcacccg acgggacaag
     1561 agattgtcgg gaacttcaga catgacaatc cgctgggcca gcccttccac gatggcagtc
     1621 tttcccacac cggattcgcc gatcagcacc ggattgttct tggaacgccg gctgaggatc
     1681 tgtaggacgc gctcgatttc atctgcgcga ccgatgaccg gatccaactg accttcctgg
     1741 gcgagctggg tcagatcacg cccaaattcg tcgagtgccg gagtgttgct ctttcggtcc
     1801 cgttcccgcg gggccgactt gcggaggaac gtgacggtga gttggcgcgc cgttaaaaga
     1861 ttggcgccca aacttctgag aatcttgccg ccgattcctt cttcttctcg cagaaggcca
     1921 agcagcagat gttcacttcc gatgtggtta tgtcccaaca agcgtgcttc ctccacgccg
     1981 tattcgatga ccttcttgac gcgggggctg aacggaatct ctccgaacgt catggtcgtc
     2041 ccaccgcccg ggagatttcg ttcgatttcg agcctgatct gctcggtcga caaccccatc
     2101 ttcttcagaa tcatcagagc gattccgtcg gattcacgca aaatggccaa gacaagatgt
     2161 tcggtcccca gatagtcatt ctggtgacgc tcggcctctt cccgtgcgag gatgatgatc
     2221 ttccggccct tgtccgtgaa tcgttcgaac atcctgcctc cttttgatgg gatctacgag
     2281 cggaactccg agagcaaacc cttcgaaaaa cttagcctaa ttctaactag accatatcca
     2341 agtgtcaagg ataccgggaa attggactcc acggaaaaga ttggcatcag actaatgaca
     2401 ttttgagcaa gccacacagg atcacttcgt tgacttcaaa agaaggctcc cgataaaatc
     2461 ccgcaccttt cgtcccacga ttcgtcttga tcacggcgcg gttcctgcga tgaaaagcaa
     2521 gagtattgga atcttaacta agcctaaatt tccagaggtc aagaccaccc tgcatgcggt
     2581 cgtcacgtgg cttcgagctc ggaacatcga agtactcctc gacacaacgt cggcaactct
     2641 tctcggtgag cccggcggca ttcagaaaac ccaattggcc agcaaggccg gcgtccttct
     2701 cgtcttaggc ggtgacggga cgatgctcaa cgctgccagg ttggcggcag aacgggggat
     2761 tccgattctg ggcgtcaata tgggcggcct gggattcttg accgaggtcc ggttagaaca
     2821 tttgtatccg tcgctggagc gggtcttcgc caatgaattt gtcctggatg agcgactcat
     2881 gctgaggacg catgtgcatc ggcacggaga gaccgtcgcg caaggtgtgg tactcaatga
     2941 tgtggtcatc agtaagggga cattagcccg catgattgag ttgaaggtgt ccatccaagg
     3001 gcagttcgtg acgaatctgc gtggagacgg gttgatcgtg agtacaccca ccggatcgac
     3061 ggcctattcc ctgtccgccg gagggcctat tattgatccc gcggtgcagt ccttaattct
     3121 tacgccaata tgtccgcaca ccctcaccca tcgcccctta atcgtaccga gcaatgcaga
     3181 gatcgacgtc acattgacca gcaaagacga cggcgcgatg acgacacttg acggtcaggt
     3241 ggggattgca atgacacaag gagacaccgt cgcgatccaa gcatcggagc atcgaacgcg
     3301 gttgatccgg tttccggaga cccactacta cgatgtgttg cgagagaagc tcaactgggg
     3361 agatcggtag ggatttgact gtgcgttgtc ctacgacaac gctttttcga ttagccctaa
     3421 catctctcga ttgctgacgt gcttgaatgc tccgatgcaa tcagccgggc ctttgtgagc
     3481 ctgctcggcg gcttcgagta cacgcaagcc tttgacattc accgggtgac tgtttcgatt
     3541 cttgagggtg gtctgaagct tgctcaatat tccctccgcg ctcgccctgc tatgcggagc
     3601 cttcgtcttg agataccgcg acacgacttc ggcgcaccac tccccatccc gtttggccac
     3661 acccaccagc ccttcgcttg ctttgcgagc ccaaccggcg agaaatacgc cgtcgaccac
     3721 tttgcctgat acctcgtcgt acgcttgaaa gagcgcatca tccggatcgt tggtcgtctt
     3781 attgggattg gtcacgaaca caccgttctt gtacggcaag ccgacggtct catcgacttt
     3841 gtcgcccacg gcgaagacca ctgagtcgca agggaactca taatactgtt tgaggccaac
     3901 cgccgccgta tcctctcctt tgggttcaag tttgttctcc tccatctcga gaccacggac
     3961 acggttgttc ccatcaacga gaatccgctt cggagaggcc aagaatctga aatccatttt
     4021 cgtgtcgctg actttcggtt cgcacttggt aaattcgtct agaacgctct tgagaatctc
     4081 ctctgctttt tgacccactg cagccagacg atccttaatg cgagcaaact cctttgtaat
     4141 gccgtccaca tccatattgg agcacacagc ccgaatttct tttggattat acttgcgctc
     4201 ggcggggcca cgtcgaacca cggccgtgac tcggtcgact ttcttatacc gaatcagcca
     4261 atgggcaata tcaaccatca catcgcctgc tccgatgacg gccacatgat tgcccatttc
     4321 aaacggtcga tcaccgaaac cgggtaagcg gttgaagtga tagaccacat ctttggcatg
     4381 gaacaccccc cgcgcgctat ctccttcgac gccgatcgtc tttgtccctt gcgctccggt
     4441 tgaaaagacg agtgcgctgg cgccaagttc ccggagatct tcaaccgtaa ggtccttgct
     4501 tttgccgacc gaaacgtttc cgaaatagtg aacgttgggc gcttccaaaa tactccagta
     4561 ggttttcttg agtcccccgc gtagcttcaa cttgctcggg aagattccgt attccgcaag
     4621 ccctccgaac ttgatgtcgc gattgaggat aatcacttcg tggccggcct ccgccatctt
     4681 atgagcgaca gccattcctg cggggccggc tcctacgacc actacaacgt gcgtttcccc
     4741 cttcgacccg atggcactca ttgataaccc ttatcttaac cctggtggaa atacgcccct
     4801 aaactgaccg aaccttcaaa attttcgcgc acattaacac agggttttgg agactgtcaa
     4861 aatgggctcc cccctcgata atcctgaaac atgttgaagg tgacgttatc cccctgcact
     4921 gccaattggg cctcccggct cagtcatgcg ccagggatcc agcaactcgt tcaaccgttt
     4981 ttccggcaag acgttctgtt ccatcgcgac ctcccgcacc gttttccctg
//
LOCUS       DATDNW010000104         4844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_831119, whole genome shotgun sequence.
ACCESSION   DATDNW010000104 DATDNW010000000
VERSION     DATDNW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831119"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VI359_02655"
     CDS             <1..586
                     /locus_tag="VI359_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY6973184.1"
                     /translation="AISMFGYGGIVLTMAIESACIPLPSEIIMPFSGYLVATGQFTMV
                     GVTLAGAIGNVVGSVAAYYAGIWGGRPFVERYGPYFLVSRKDLDLADRWFQKYGEAAV
                     FFSRMLPVVRTFISLPAGVARMNFPRFVLFTFIGALPWCYLLAYIGLKMGERWDQLRD
                     YFHQFDIVIGLGLALVIGYFLWSHWPRRQPNLES"
     gene            594..2069
                     /gene="cysS"
                     /locus_tag="VI359_02660"
     CDS             594..2069
                     /gene="cysS"
                     /locus_tag="VI359_02660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY6973185.1"
                     /translation="MLTIYNTLTGKKEPFQSLVPKTVRMYVCGVTVYDYCHLGHARSA
                     LVFDMIRRYLEYAGYTVEFVKNFTDVDDKIIKRANEQGVSCDAITTKYIQAYYEDMDK
                     LGVRRASKEPKATESMVDIIALIGQLVKKDIAYRVDGDVYFQVEKYPAYGRLSKRKLE
                     DLQAGARVEVDERKRHPMDFALWKSSKPGEPSWPSPWGSGRPGWHIECSAMSMQYLGE
                     TFDIHGGGMDLIFPHHENEIAQSSGATGKEFARYWIHNGFVQINQEKMSKSLGNFFTI
                     REIFEKSEWSEEITGEVLRYFLHSTQYRGPLDFSDQSLREAKNALEGFYDLFKRLSEP
                     EQRTAGDSTLVSAVDRARMAYNQAMDDDFNTPMALAVIQNLRGDVNKLLERGVSTETR
                     QRARDAFRELGNVLGLFQLEEWRFNPITIHVSEEISVGVVGRAKVKDVISDTDIEVKL
                     KERNEARKQKNFKKADEIRQFLASHGIIIEDKPDGTSRWKR"
     gene            2080..2835
                     /gene="rlmB"
                     /locus_tag="VI359_02665"
     CDS             2080..2835
                     /gene="rlmB"
                     /locus_tag="VI359_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY6973186.1"
                     /translation="MLYGLHAVREALRAKTRPLQRVLVLRIDRQFADLVQLAKAQQVP
                     VHIEPQPAFDRLVPHGRHQGVIAFVAAKSYSSTEDILQRAKERDEPPFLVLLDGVEDP
                     HNLGAALRTAEAAGVHGVFIPERRAVGLTSVVAKVSAGALDHILVSRVGNLARLIEDL
                     KKVGVWVYAVEPSAQKQITQLDLCGSVALVFGGEGQGVRPGVLQLCDDRVSIPMKGKV
                     ESLNVSAAVAVALFEAVRQRGESGSRTKTLTES"
     gene            complement(2822..3097)
                     /locus_tag="VI359_02670"
     CDS             complement(2822..3097)
                     /locus_tag="VI359_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY6973187.1"
                     /translation="MARSAKAITRLSLADLTPPPRKKRPESLTSRELEILELIWAGFK
                     NKEIGQRLKISVKTVEAHRANMMKKMRVSNTAQLLKTAIQGGMIRIR"
     gene            3308..4099
                     /locus_tag="VI359_02675"
     CDS             3308..4099
                     /locus_tag="VI359_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY6973188.1"
                     /translation="MPAMIELPDYLITGLIGVIVGSFLNVCIYRLPRHEWIIWPGSHC
                     PSCGKPIAWYDNVPLISYLLLLGRCRSCRAPISLRYPIVEAANAAGYLAILWHFGLNG
                     TSVLYAGLYSALIVVTGTDLSHKIIPNVITYPGIIVGLLGAVTVLPVGIVNSLLGIAV
                     GGGLLWILAWASPYMFGKEGMGGGDIKLLAMIGAFLGWKPALLTIMIGSLIGSVIGIG
                     LMVARIIKRDEHIPFGPFLVVGAVLSMFFAQPLLVWYQGLFNTGY"
     gene            4385..4828
                     /locus_tag="VI359_02680"
     CDS             4385..4828
                     /locus_tag="VI359_02680"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY6973189.1"
                     /translation="MEVFEDEQGKSFTEILVVLAVIGLMAGLAGPSFVGIIARVEVRS
                     AASEIASELRLARQLAMARRERLRVVFDGTSRTITLQRADAEGVLDVYRYSDKGIVVD
                     EPSAGPEVLFHPTGRSATATTIVVRDKQGCVTKLTLSLTGRVAFS"
BASE COUNT         1050 a         1221 c         1389 g         1184 t
ORIGIN      
        1 ggcgatttca atgttcggct atggcgggat tgtcctgaca atggcgatcg aaagtgcctg
       61 cattcctttg cccagtgaga tcatcatgcc attctccggt tacctagtgg cgaccggaca
      121 atttacgatg gtaggcgtga cgctcgccgg cgcgatcggc aatgtcgtgg gatcggtggc
      181 ggcgtactat gccgggatct ggggcggtcg gccgttcgtc gagcggtatg ggccgtattt
      241 cctcgtgtcg cgcaaagacc tcgaccttgc cgatcgctgg tttcagaagt acggagaagc
      301 tgccgtgttc tttagccgga tgttgccggt agtccgcacc ttcatctcac ttccggcggg
      361 agtcgcacgg atgaattttc cacggttcgt gctgtttacg tttatcgggg cgttaccctg
      421 gtgttatctc ttggcgtata tcggcctgaa gatgggcgaa cggtgggatc agttacggga
      481 ctactttcat caattcgaca ttgtgatcgg tctcgggctg gccttggtca tcggttactt
      541 cttgtggtct cattggccaa ggcgccagcc gaatttagag tcgtaatcat cagatgctaa
      601 cgatctacaa taccttgact ggcaagaagg aaccgtttca gtcgttggtt cccaagaccg
      661 tgcgcatgta tgtgtgcggt gtcacggtct atgactattg ccatctgggt catgcccgca
      721 gcgccctcgt cttcgacatg atccgtcgat acctggagta tgcagggtac acggtcgagt
      781 tcgtgaagaa cttcacagat gtggatgaca agatcatcaa gcgagccaac gagcaaggag
      841 tttcctgcga cgcgattacg acgaagtata ttcaggccta ttacgaagat atggacaagt
      901 taggtgtccg ccgcgcttcg aaagagccga aagcgacgga gtccatggtc gacatcatcg
      961 cgctcatcgg ccaactggtg aaaaaagaca tcgcttatcg agtggacggc gatgtgtatt
     1021 ttcaggtgga aaagtatcct gcctatggcc gcctgtccaa gcgcaaactc gaggatcttc
     1081 aagcgggggc gcgtgtcgag gtcgatgaac ggaagcgcca cccgatggat ttcgcgttat
     1141 ggaagagcag taagcccggt gaaccttcat ggccgagtcc ctggggttcc gggcgaccag
     1201 gctggcacat cgaatgctcc gcgatgtcga tgcaatactt gggcgagacg ttcgatatcc
     1261 acggcggggg catggacctg atttttccgc atcatgagaa cgaaatcgcg cagtcgtccg
     1321 gcgcgacggg caaggaattc gctcgttact ggattcacaa cggcttcgtt caaataaatc
     1381 aagagaagat gtctaagtca ctcggcaact tcttcaccat tcgagagatc ttcgaaaagt
     1441 cggaatggtc ggaagagatc acaggagaag tgttgcgata ttttctccac tctacgcagt
     1501 atcgagggcc gctcgacttt tcggatcaaa gcttacgaga agcgaagaat gcgttggaag
     1561 ggttctacga tctgtttaag cgattgagtg agccagagca acggacagct ggtgacagca
     1621 cgcttgtgtc cgcggtcgat cgtgccagga tggcctacaa ccaggcaatg gatgatgatt
     1681 tcaatacgcc gatggctctt gcagtgattc aaaatctgcg aggggacgta aataagcttc
     1741 ttgaaagggg cgtctctact gagactcgtc aacgggcccg cgacgccttt cgggaactcg
     1801 ggaatgtcct gggtttgttc caattggagg agtggcggtt taacccaata acaatccatg
     1861 tatctgagga gatttccgtc ggggttgtgg gtcgtgccaa agttaaagac gttattagtg
     1921 acactgacat tgaagtgaag ttgaaagagc gcaatgaggc cagaaaacag aagaacttca
     1981 agaaggccga cgagattcgt cagttcctcg cgtcccacgg catcatcatc gaggataagc
     2041 cagacggcac cagccggtgg aagcggtagc ccgccggatc tgctgtacgg tttgcatgcc
     2101 gttcgcgaag cacttcgcgc gaagactcgc cctcttcaac gggtgctcgt tcttcgcata
     2161 gatcggcaat ttgccgatct tgtccagctc gcaaaggcgc aacaagttcc ggtccacatc
     2221 gagcctcaac cggcctttga ccgcttggtc cctcatggaa ggcatcaagg tgtcatcgca
     2281 ttcgtcgcgg caaaatccta cagcagcacg gaggacattc tccaacgggc gaaggagcgg
     2341 gatgaaccgc cctttcttgt actcctcgat ggagtcgaag atcctcataa cctcggggcg
     2401 gcgttgcgca cggctgaagc ggctggagtg cacggggtat tcatccccga acgccgggcc
     2461 gtcggcctca cctccgtcgt cgccaaagtg tccgccggtg cgctcgatca tatcctggtg
     2521 agtcgggtcg ggaatctcgc tcgattgatc gaggatctga agaaggtcgg agtgtgggtc
     2581 tacgcggtcg agccgtcagc gcagaagcag atcacgcagc ttgatttatg cgggtccgtt
     2641 gctctggtct ttgggggcga aggacaaggc gttcgcccgg gcgtgcttca gctgtgtgac
     2701 gatcgggtta gcattccgat gaaggggaag gtcgagtcct tgaacgtatc agccgccgtc
     2761 gccgtggcgc tcttcgaagc ggtgagacag cggggagaat ccggttcgcg aacaaagaca
     2821 cttaccgaat cctgatcatt ccgccctgga tggcagtttt cagcagttgg gccgtgttcg
     2881 agacgcgcat ttttttcatc atattcgccc gatgcgcctc gacggtcttg acgctgatct
     2941 tcaaccgctg accgatttcc ttgttcttga atccagccca aatcagttca agaatttcca
     3001 attcgcggct ggtcagcgac tccgggcgct tcttccgggg aggcggtgtg aggtcggcaa
     3061 gagaaagcct ggtgattgct ttcgcgcttc gtgccatgat tcaacttctc cttcattaga
     3121 tggaaaaata agatatagtg gtttcacata agagaagctg gagaatgaat acctggccaa
     3181 accggtaggg attataggga atgttgctac cgcatgtaaa tggaagcatt atggctctag
     3241 gtgtactacg taatgtatct ttgttgaatc gggcatttcc cctgtgggca cgattctgtc
     3301 tgtcgatatg ccagctatga ttgagctgcc ggactacttg attactggac tgatcggcgt
     3361 catcgtcggc agctttctca atgtctgtat ctaccgtttg ccgcgtcatg agtggattat
     3421 ctggcctggc tcccattgcc cttcctgcgg aaaaccgatc gcatggtacg acaatgttcc
     3481 tctcatcagc tatctcctcc tcctaggtcg ttgtcgctct tgcagagcgc cgatctcgct
     3541 tcgctatcca attgtagagg cggctaatgc agcaggatat ctcgccattc tatggcactt
     3601 cggcctcaat ggaacgtcgg tactttatgc cggactctac tccgcgctga tcgtcgtgac
     3661 cgggaccgat ctctcccata agattattcc caacgtgatc acctacccgg ggattatcgt
     3721 cggattgctc ggagctgtaa cagtccttcc tgtaggtatc gtgaattcat tgctcggaat
     3781 tgccgtaggc ggaggactgc tgtggatttt ggcctgggcg agcccgtata tgttcggaaa
     3841 agaagggatg ggaggcggtg atattaagtt gctcgcgatg atcggggcct ttttgggatg
     3901 gaagcccgcg ctcctgacca tcatgatcgg ctcgctgatc ggatccgtca taggcatcgg
     3961 cttgatggtc gcccgaatta tcaaacggga cgaacacatt ccatttgggc cgttccttgt
     4021 agtcggcgcg gtactctcca tgttctttgc ccaaccgctt ctcgtctggt accagggact
     4081 attcaacact ggatattaga cgtttccctt ccatcgcgta gtctcctgta tctctttcga
     4141 tgaacctctg tatcttaatt ggagaaggaa atagcacgtt cacctgtctc tcagtgagtt
     4201 cctgcgaata gagccacgcc cagttaaccc cttttatccc aataaagtgc caagaatctg
     4261 acaagaaagc ttgtgcgtgt tcctgtcgga ccattacgac cagggactca acctgcgagg
     4321 cacagctttt ggatttgcct caatcatctg gcgtgatcat tctcggactt ttacgtgtat
     4381 cgaaatggaa gtatttgagg atgagcaggg gaaaagcttc acggagatac ttgtcgtctt
     4441 ggcggtgatt ggcctcatgg caggcctagc cggtccaagc tttgttggga taatagcccg
     4501 ggtcgaggta cgaagtgcag catctgaaat tgcttctgaa ttgagactgg ccaggcaact
     4561 ggcaatggca cggcgtgagc gcctccgtgt cgtcttcgat ggaacgtctc ggacaataac
     4621 cctacagcgt gccgatgcag aaggcgtgct ggatgtctat cgctatagcg ataagggaat
     4681 tgtggttgac gaaccgagcg ctggcccaga agtgctcttt catcctaccg ggcgatcggc
     4741 gacggcgacg acgattgtag tacgggataa acaggggtgc gtcacgaaac tcacgctaag
     4801 cctaacggga agagtggcgt tctcatgatg accaatcacg gaca
//
LOCUS       DATDNW010000105         5175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1171921, whole genome shotgun sequence.
ACCESSION   DATDNW010000105 DATDNW010000000
VERSION     DATDNW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171921"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="bioB"
                     /locus_tag="VI359_02685"
     CDS             <1..937
                     /gene="bioB"
                     /locus_tag="VI359_02685"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY6973190.1"
                     /translation="ECQTVLNTPDDDLLALLQAAFTVRSRYFGKTVRLQMLQNAKSGA
                     CQEDCHYCSQSAVSTAQIERYNLLPQEQMIEGARRAAASKAQRYCIVISGRSPLDREI
                     DEIAGAVRAVKQEIPIQICCSLGLMNEAQARRLKAAGVDRINHNLNTSEAYHASICTT
                     HTFQDRLRTIQNARAAGLEICSGGIVGMGEKDEDLIDLAMALRDVKPDSIPVNTLHPV
                     AGTPLEHCDQLTPQRCLKVLCLFRFLHPRTEIRIAGGREHNLRSLQPLALYPADSVFV
                     NGYLTTPGQPAPEVWGMIKDLGFTIEVDYQQPVAS"
     gene            1318..1521
                     /locus_tag="VI359_02690"
     CDS             1318..1521
                     /locus_tag="VI359_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973191.1"
                     /translation="MNMIRLLREGKIGAVNVLPFAHDPANLYETRLNVLDELKGCEQK
                     EQVQAVLGQCTDCDVREWLDIKP"
     gene            complement(1528..4146)
                     /locus_tag="VI359_02695"
     CDS             complement(1528..4146)
                     /locus_tag="VI359_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6973192.1"
                     /translation="MIPSTCLFWVLFRTHLAQRPFRVVLSLLGVSLGVMASVAMTTAN
                     VDVLRSFEQAVTEVAGPATLEVLAGDLGVDETVITTVRAVPGVTAAVPVIEESVAMVQ
                     GAHRGESVQLFGLDLIAEAGTRGFRLHTAGGQGALDQLLDSDTAYVGPKLAEEWHLTV
                     GSPFEVMAGGRLVRLQVGGVLHSETARPSVWDRAMLMDIAAAQVLFGSIGRLDRIEVV
                     TIQGRPVEDIQSALRAALPPHLTVQRPTQRTKQVEQMVRAFQLNVRVLSWVGLLVGAF
                     LIYNTTAFMVARRRREIGIYRALGMTERRVAALFLAEAGFLGMLGGVVGGTCGVLLAR
                     SLVSLVSQTISDLYVPVSSGPSALTFNYDLWSPLIQGVLLGTVVSMTGAWGPSLDASR
                     TITVRALAPGDYEVTQQLRLARFAWTAGVLSFLAAVLSLLNPVAGLPVFGYLATLCLL
                     VALSCLAPVCIQVLGSSRRRDEAVIHGLGGSLRRIAVDHAARHPGRNGVTVSALMVGM
                     AIMIGVVVMVRSFRQTVEIWVNETVIADMVVAPPAWLHGRHAGQASRALPGTWATKLA
                     SIDGVAAVDTYRDVHVDVQGRSVVLISRDLRLHAERSRYLVIEGDSATVLRRAAETGG
                     VLVSEVLANRLGVREGMSIAVTTPSGVKNVPIAARFYDYATDGGKMVMDRAAYQALWH
                     DDRVTVFPIYLHAGADVDAIRQSLRQRLAMDEEGSVSPLIISNAELRREILNIFDRTF
                     ILTYVLEAIAVLIAVLGIVNTLVTAVLERRRELATLQAIGASPAQVKQLVLWEAAYLG
                     LLGSVLGVIGGVLLAVILIKVINKQSFGWTIQMTVPLGVLVQSIILALGAALLAGYWP
                     ARWAANQPVVEGLREE"
     gene            4318..4590
                     /locus_tag="VI359_02700"
     CDS             4318..4590
                     /locus_tag="VI359_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973193.1"
                     /translation="MGARAAAQRKHQTMPEDTAIEQLTPRQRDILRLVALGHTNREIA
                     TLLEISVRTVEVHRFNLMRRLEVRNVAQLLRRALQMGLLSKNFGTK"
     gene            complement(4593..>5175)
                     /locus_tag="VI359_02705"
     CDS             complement(4593..>5175)
                     /locus_tag="VI359_02705"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="catalyzes the conversion of dihydroorotate to
                     orotate in the pyrimidine biosynthesis pathway; subclass
                     1A is a dimer formed by two identical PyrD subunits each
                     containing an FMN group; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEY6973194.1"
                     /translation="SGPDLIEVNLSCPNIPGKPQIGYDAEASERLLKRLRRIITVPMG
                     VKLPPYFDPAHHKVMGDMIGRCGVDFLNLINSVGNGLVVDPEREAAVIKPKGGFGGLG
                     GAIIKPVALANVRAFWKIFEGRMPIIGTGGVMDGMDAFEHFLCGASAIQVGTVLVEEG
                     IEVFGRLEMELTAQLTQKGYRTPADCRGKLKEL"
BASE COUNT         1189 a         1603 c         1368 g         1015 t
ORIGIN      
        1 ggagtgtcag accgtcttga atacgccgga tgacgacctg ctcgcgcttc tccaggctgc
       61 cttcactgtc cgttcccgct actttgggaa aactgttcgc ctgcaaatgc tgcagaacgc
      121 caagagcggc gcgtgccagg aggactgcca ctactgttcg caatcggccg tctccaccgc
      181 gcaaatcgaa cggtacaatc tactgcctca ggaacagatg atcgaaggag cgcgacgggc
      241 tgccgcctcg aaggcccagc ggtattgcat cgtcatcagc ggcagaagtc cattggatcg
      301 cgagatcgat gaaatcgccg gagccgtccg agctgttaaa caagagattc ctattcagat
      361 ttgttgttca ctcggcctca tgaatgaagc tcaggcgagg cggctgaaag ccgcgggcgt
      421 ggatcggatc aaccacaatc tgaataccag cgaggcctac cacgcctcaa tttgcacgac
      481 gcatacgttt caggatcggc tcaggacgat tcaaaacgcg cgggccgcgg gtctcgaaat
      541 ctgctccggt ggcatcgtgg ggatgggtga gaaagacgag gacctcatcg acctggccat
      601 ggccctgcgc gacgtgaagc cggattcgat tccggtgaac accctccacc ctgtcgcagg
      661 gacaccttta gaacattgcg atcaacttac cccgcaacga tgccttaaag tcctgtgctt
      721 gttccgattc ctccacccac gaactgaaat ccgtatcgcc ggcggacggg aacataatct
      781 ccgaagcctc caaccgctgg cgctataccc ggcagactct gtcttcgtga atggctacct
      841 gacaacacct ggtcagccag ctccggaagt ctggggcatg attaaggatt tgggattcac
      901 gattgaggtc gattaccagc agccggtggc gagctgaatc cttctcctcg tgtttttctg
      961 cctcatgcga agcgctccgc acaatcgaaa ggtattcacg tcgtaggcga ggctccattg
     1021 cgacacatga ttcaacgaat cctcatatgt ctggctcttg ctagcgtatt tcttctacag
     1081 gtgcatcttg gcccttctcg ttcagccgaa attgagccct tgaggcacga cgagatgctc
     1141 gcccagtgcg ccgatcgaat tgaaaagtgg tttgcgcttg tcacgatccg atcccaggaa
     1201 cttgacttcg atcatgcacg agtcatctta gtgggtccga ccgttctcat ccctcttaag
     1261 gccgcacatg ggcatgtgag ggctatacgg tggcgtccag gagatgtctc gcaagcaatg
     1321 aacatgattc gattgcttcg tgaaggcaag atcggtgctg tgaatgtgct gcctttcgct
     1381 catgatccag ccaatctgta cgagacgcgc ctgaatgttt tagacgaact gaaagggtgt
     1441 gaacaaaagg agcaggttca ggcggtcctc ggacaatgca cagactgcga cgtacgcgaa
     1501 tggctggaca tcaaaccgta aggcttttta ttcttctcgt aacccttcga cgactggttg
     1561 attagcagcc catcgggcgg gccagtagcc tgctaggagt gccgccccaa gcgctaagat
     1621 gatggactgc acgagaacgc cgagcggcac cgtcatctga atcgtccagc cgaaggattg
     1681 cttgttgatc accttgatga gtatgacggc caacagtact ccccctatta ctccaagcac
     1741 cgatccgagc agcccgagat acgccgcttc ccacagcacc aattgcttca cctgagcggg
     1801 acttgcgccg atcgcttgca gcgttgctaa ctcccgccgt cgttccagta ccgcggtgac
     1861 caacgtgttc acgatgccca acacggcaat taacaccgcg atggcttcga gtacatacgt
     1921 caatatgaag gtacggtcga agatgttgag aatctcacgg cgcaactcgg cgttgctgat
     1981 gatgagcggc gagacgctcc cctcttcgtc catcgccagt cgttgcctaa gcgactggcg
     2041 aattgcatcc acatcggccc ctgcgtgcag atagatcgga aagacggtca cgcggtcgtc
     2101 atgccagagg gcctgatacg ccgcccggtc catgaccatt ttccccccat cggtcgcata
     2161 atcgtagaac cttgcggcga ttggtacgtt cttgacacca gaaggcgtcg tgacggcaat
     2221 agacattcct tcccggacac cgagtcgatt ggcgagcact tccgacacca agactccgcc
     2281 ggtctcggcg gcacgtctta gtacggtggc tgaatcgccc tcgatcacga gatagcgact
     2341 gcgctcggca tgaagccgga ggtcccttga gatgagcaca accgaccggc cctgcacgtc
     2401 cacatgcaca tcgcgatagg tatcgaccgc cgccacgccg tcgatcgatg ccaatttcgt
     2461 ggcccatgtt cccggcaatg cccgcgacgc ctgacccgcg tgccgaccgt gcaaccaggc
     2521 cggcggagcc acgaccatat ctgcaatcac agtttcattg acccagattt ccaccgtttg
     2581 gcgaaagctc cgaaccatca ccacaacacc aatcatgatc gccattccga ccattaaggc
     2641 cgagactgtc acgccattgc gaccagggtg gcgcgctgca tggtcgaccg caatccgcct
     2701 caaactgcct ccgagcccat gtatgacagc ctcgtcacgc cgacgagacg atccaagcac
     2761 ttggatacag accggtgcaa ggcaagagag agccaccaag agacacaacg tggcgagata
     2821 cccaaacacc ggaagtccag cgacaggatt cagcagggat aagaccgcag caaggaaaga
     2881 gaggaccccc gccgtccatg cgaatcgtgc gagccgaagt tgctgcgtca cttcataatc
     2941 ccctggggcc aaggctcgta ccgtaatggt ccggcttgca tccaggctcg gtccccacgc
     3001 cccggtcata gagacgaccg tgcccaagag aaccccctga atgagcggtg accacagatc
     3061 gtaattgaag gtcaacgccg aagggcccga tgagaccggc acataaagat ccgagatcgt
     3121 ctgactgaca agagagacca gactgcgggc aagtagaacc ccgcaggtgc ctccgacgac
     3181 tccccccagc atccctaaga aaccggcctc ggcgagaaac aacgcggcga ctcgtcgttc
     3241 cgtcatgcca agggcacggt agatcccgat ctcacgtcgt cgccgagcca ccatgaaggc
     3301 cgttgtgttg taaatgagga aggctcccac gagcagtcca acccaactca acacgcgcac
     3361 gttgagctga aacgcccgca ccatctgctc gacctgcttc gttcgttggg tcggtcgttg
     3421 gacggtcaga tggggaggca gcgcggcccg taaagcagat tgtatgtcct ccaccggacg
     3481 gccttgtatc gtgaccacct cgattcggtc caaccgtccg atcgatccga acagtacctg
     3541 tgcagcagcg atgtccatga gcattgcccg atcccagacc gatgggcgcg ccgtttcact
     3601 atgaagcact cccccaactt gcaaccgcac gaggcgtccg cccgccatca cctcgaaggg
     3661 actcccgacg gtgaggtgcc attcttcagc cagctttggt ccaacatagg ccgtatcgga
     3721 atccagtagt tggtctaacg ctccctgacc tccggctgtg tgaagccgaa agccgcgcgt
     3781 gccagcttct gcaattaagt ccaacccaaa cagctgcacc gattcaccac ggtgcgctcc
     3841 ctgcaccatc gcgacactct cctcgatgac tgggaccgcg gccgtcactc ctggcactgc
     3901 ccgtaccgtt gtgattaccg tctcatcaac tcccagatct cctgcaagca cttccaacgt
     3961 ggctggccca gcgacctccg taactgcctg ctcgaacgag cgaagaacgt cgacgttcgc
     4021 tgtcgtcata gccactgacg ccatgactcc taacgacact cccaagaggc tgagcacgac
     4081 gcgaaagggg cgctgcgcga gatgggtacg gaagagcacc cagaaaagac aggttgaagg
     4141 gatcatacga tgccgcaacc cagcacggac ggcgggaagc gagtgcacat caacatggaa
     4201 caaaattggc tagtttcgcc tcgataaatg gtcctgcaca aattagtagt ttcgtttaca
     4261 gagttaccca cctttgctag actgccttcc agccattgaa ttcggaggaa cagccctgtg
     4321 ggagccagag ccgccgctca acgcaagcac caaactatgc ccgaagacac tgcgatcgaa
     4381 caactgacac cccgtcaacg ggacatcctc cgccttgttg cactcggcca tacgaaccgc
     4441 gagatcgcga ccttgttgga gatcagtgtg cgaacggtcg aagttcaccg cttcaatttg
     4501 atgcggcggt tggaagtccg gaacgttgcg caattgctcc gacgggccct gcagatgggc
     4561 ttgctctcaa agaacttcgg aaccaaatag tcttacagct ccttcaattt cccgcgacag
     4621 tccgccggcg tccgatatcc cttttgagtg agttgagcgg tcaactccat ttcaagacga
     4681 ccaaacacct cgattccttc ctccaccaac accgttccga cctgaatggc ggaagccccg
     4741 cataagaaat gctcgaacgc atccatccca tccatgacac caccagtgcc tataattggc
     4801 atgcgccctt cgaaaatctt ccagaaggca cggacattcg cgagggcgac cggtttaatg
     4861 atggccccgc cgagtccacc gaacccgcct ttcggtttaa tcactgccgc ctcgcgctcc
     4921 ggatcgacaa cgagtccgtt accaaccgag ttaatcaaat tgaggaaatc aacgccgcac
     4981 cgaccaatca tatcccccat caccttgtga tgtgcgggat cgaaataggg ggggagtttg
     5041 acgcccatcg gcacggtaat gattctccgc agacgcttga ggagccgttc agaggcctcg
     5101 gcatcgtaac cgatctgggg cttgccggga atgttcgggc aggacaggtt cacttcgatc
     5161 agatcgggcc cgctc
//
LOCUS       DATDNW010000106         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_283180, whole genome shotgun sequence.
ACCESSION   DATDNW010000106 DATDNW010000000
VERSION     DATDNW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283180"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VI359_02710"
     CDS             <1..615
                     /locus_tag="VI359_02710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6973195.1"
                     /translation="RQELNIPPDARVMIAGSTHPGEEDMIRTVFSRLRAGFPSLRLIL
                     VPRRPERGVEVQNVFRNDGVEVSLLSQPTGSPPVVVVVDRMGYLSRLYALADIAVVGG
                     SFVPKGGQNPIEPAACGKPVLFGPDMHDFPDVSRWLLEARGALQVRNGEELYTECHRL
                     LADPQQARAMGDRARRVVTENQGTTARIVADILGLAHSSSAHSP"
     gene            complement(733..1518)
                     /locus_tag="VI359_02715"
     CDS             complement(733..1518)
                     /locus_tag="VI359_02715"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6973196.1"
                     /translation="MDVGAHTSLTGRTQEDSAAMRITIIVLGTMLAGLFLILPNLAHA
                     DQDLETAKLLIKMVELGRGVISEQQALINDPAKGDKGVTADFVTSQIADRFKKQTNVD
                     LMAPNSHPSIKLLQTMLESQKEVMDGAQPMINKQGVGFKAFLPAVFARRTGATFYKKT
                     GIQVKLTSVAYRYDGNKPDDFEQEVLQVFADPKTPKGQVVSRTSMVNGKPALRVMKPE
                     YAGASCLSCHGNPKGERDITGMRKEGWNEGDLAGAISIVMPAK"
     gene            complement(1865..2005)
                     /locus_tag="VI359_02720"
     CDS             complement(1865..2005)
                     /locus_tag="VI359_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973197.1"
                     /translation="MSLSARDIIAVGIAREVIHKYNVERMDRAQRRAEGILMQYRYQR
                     NY"
BASE COUNT          430 a          589 c          606 g          500 t
ORIGIN      
        1 cgccaggaac tcaacattcc gccggatgcg cgtgtcatga ttgccgggag cacgcatccg
       61 ggggaagaag acatgattcg caccgtgttt tcccggctga gagcagggtt tccttccctt
      121 cggttgatcc tcgtgcctcg ccgaccggag cgaggcgtcg aagttcagaa tgtgtttcgg
      181 aacgatggcg tggaggtgtc actgctctct caaccgacag gctcacctcc tgtggtcgtc
      241 gtcgtggatc ggatgggcta tctcagccgc ttgtacgcgc tggccgacat cgccgtggtc
      301 ggtgggagtt tcgttcccaa gggcgggcag aatccgatag aaccggccgc ttgcgggaaa
      361 cccgtcctct ttgggcctga catgcacgac tttccggatg tgtcccgatg gttgttggag
      421 gccagagggg cccttcaagt acggaatggg gaagaattat atacagagtg tcatcggttg
      481 ctagcggacc cgcaacaggc cagggccatg ggagaccgcg cccgtcgcgt cgtgacggag
      541 aatcaaggca ccaccgcgag aatcgtcgca gacattctag gccttgccca cagctcctcg
      601 gctcattcgc cctagtctct tccgctaatc tgaaaattcc cgaggaatgc ggtgattgtt
      661 tgagtgggtg gccgctcccc cgtcgtaagc tcacatgggg gagcggtcgt ggtgagtggc
      721 gcgaaacgac gattattttg ccggcatgac gatgctgatg gcgccggcca gatctccttc
      781 attccaacct tcttttctca tcccggtgat gtcccgttct cccttcgggt tgccgtggca
      841 gctgaggcac gaggccccgg cgtattccgg cttcatgacg cgaagcgccg gtttgccgtt
      901 caccatgctg gtgcggctga cgacctgtcc cttgggagtc ttcgggtccg caaacacctg
      961 cagcacctcc tgctcaaaat cgtccggctt gttcccatca taccggtaag cgacgctggt
     1021 caacttcacc tgaatgcctg ttttcttata gaaggtcgcc ccggttcggc gtgcgaagac
     1081 ggccggcagg aacgctttga acccgactcc ttgcttgttg atcatcggct gggctccgtc
     1141 catcacttcc ttttgcgatt ccagcatggt ttgtaacaac ttgatcgatg gatgagaatt
     1201 tggagccatg agatcgacat tcgtttgctt cttaaaccga tcggcgatct ggctggtcac
     1261 aaaatcagcg gtgacaccct tgtcgccctt tgcaggatcg ttgatcaagg cttgctgctc
     1321 cgagatcacg ccacggccga gctcgaccat cttaatcagc agcttggctg tctcaagatc
     1381 ctgatcggcg tgtgccagat tcgggaggat aagaaacagt ccggctaaca tagttccgag
     1441 tacaatgatt gttattcgca tcgccgctga gtcctcctgt gttcgcccgg tgagtgatgt
     1501 gtgcgccccg acatccatcg aagcaacctc ctaaagaagt ccggtcacgt cgcaagaatg
     1561 tacgtgcatc tacgagtata gcaggcgata aaaaactgtc tcgtcgggaa tgttttgatc
     1621 gaattaccag aataatctgg gctagaaacg cgggcgggaa tgatgaacta actcattgca
     1681 atacaatacg ttacggcgtc ttgaaaccca tcactgtgcg aggtttcccc tttttgataa
     1741 tcccctaaga accgaggggg ggaccttcaa gacactggag ggaatcggta cgactaccgg
     1801 tactggccgg cgaggtctct gaccgaccta ccagcgacca ttcgatacat gaacatccgg
     1861 tgggttagta attacgctgg tagcggtact gcatcaggat gccttcagct cgccgttgag
     1921 cgcgatccat ccgctccacg ttgtacttgt gaatcacttc tctggcgatg ccgacggcta
     1981 tgatatcgcg cgcggagaga gacatcggct cccctttggt tgagtcacgc gattatagct
     2041 gaatttaggg ggttctcgta ccttacgagc cgagatgctg aatcgcgacc gcttcgacac
     2101 agacacggag gggaatatta tcggg
//
LOCUS       DATDNW010000107         4389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_626623, whole genome shotgun sequence.
ACCESSION   DATDNW010000107 DATDNW010000000
VERSION     DATDNW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626623"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VI359_02725"
     CDS             complement(<1..467)
                     /locus_tag="VI359_02725"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY6973198.1"
                     /translation="MMTTIARGTALCMTAVWLAMPGAAGAADREKLLSEPGVYGTFVA
                     FGFDEEWAKEDQPTRIAHLTVLKGVVEQHREKLAIDLYLLRGLSDHADLLFRVHATEL
                     RDTQKFLVDLQASPFGKHLQTTVVLHGLTKKANYVPGFPDNLKTELKAPSESGP"
     gene            complement(464..1774)
                     /gene="purB"
                     /locus_tag="VI359_02730"
     CDS             complement(464..1774)
                     /gene="purB"
                     /locus_tag="VI359_02730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY6973199.1"
                     /translation="MIDRYSRPKMKALWELKHKYEIWLEVELQACAAFERAGQVPRGT
                     SAKIRKKARINVDVIAEIEKVTKHDVIAFLESLMESVGPEHRFLHMGLTSSDIVDTSL
                     AIQMTEALDMILEDLDGLLTVLQRQAIRYKNTVMVGRSHGIHGEPISFGFKLAIWYEE
                     VCRHRERLGRVRDDIAVGKLSGAMGTFAHQGPDIEEYVCAKLGLKPDPVSSQVVQRDR
                     HAAYATALALLAASIEKFATEIRHLQRTEVLEAEEYFTEGQKGSSAMPHKRNPIASEN
                     LCGLARVVRANSLAAMENVALWHERDISHSSVERVIMPDSTILVDYMLARITDVIKNL
                     VVYPATMKRNLELTGGLVYSQRLLLALIEKGAQRKESYEAVQKNAMASWNGEGDLQTL
                     VSKDSFITKHLKASEIKACFNPKYYLRHIDQIYRRVFGPRGKKR"
     gene            complement(1866..2330)
                     /gene="nusB"
                     /locus_tag="VI359_02735"
     CDS             complement(1866..2330)
                     /gene="nusB"
                     /locus_tag="VI359_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY6973200.1"
                     /translation="MGSRHQAREHALQILFQYDIHGKPDVWLDEFWKTVKVTDEVKAF
                     TQQIVQGVLEHKKELDALIGKHATNWKVSRMPIVDRNILRAGLYELLWMGEVPAKVTL
                     NEAIELAKSFGDDEASKFVNGILDKVLMSDPRLDVKRAEAAGVKREVERRNA"
     gene            complement(2333..2878)
                     /gene="ribH"
                     /locus_tag="VI359_02740"
     CDS             complement(2333..2878)
                     /gene="ribH"
                     /locus_tag="VI359_02740"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY6973201.1"
                     /translation="MKLRKGSHDATGLRFGIVVSKFNKFVTSKLLSSCAEALTKHGVK
                     ADDIEVVRVPGAFEIPIVARTMARSQQFHAVICLGAVIRGDTPHFDYISAEASRGIGQ
                     AALDADVPVIFGVLTTDTVAQAIERADPAKFNRGGEAAKSAIEMASVMRLLKTTDQAQ
                     STKAVAASNGRGRPVRRRSKT"
     gene            complement(3022..4230)
                     /locus_tag="VI359_02745"
     CDS             complement(3022..4230)
                     /locus_tag="VI359_02745"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY6973202.1"
                     /translation="MPTPFNSIEDAIRDIKKGKFVILVDDEDRENEGDLVIAAEKVTP
                     QSINFMAKHARGLICLALTPERVDELHLPQQAVENTATFGTAFTVSIDARKDITTGIS
                     AADRATTIHVAIDPKSKPTDLARPGHIFPLKAQQGGVLRRAGQTEGSVDLARLAGLQP
                     AGVICEIMNEDGTMARVPELAKFAKAHKLKMVTVKALIEYRMRRETFVKRMASATLPT
                     TFGEFEAVAFENEIDNVTHIALVKGRVDSGAPTLVRVHSGCLTADALGSLRCDCRDQL
                     HKAMEIIQKEGRGVLLYLNQEGRGIGLLNKIKAYSLQDQGSDTVEANLKLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLKVVERVPIEIQPRDANVKYLRTKK
                     QKLGHILKKV"
BASE COUNT          859 a         1386 c         1122 g         1022 t
ORIGIN      
        1 ggacctgatt cactcggcgc ttttaattcc gtcttcagat tatcaggaaa gcctggaacg
       61 tagttggctt tcttggtcag gccatgcaag accacggtcg tctgtaaatg ctttccgaac
      121 gggctggcct gaagatccac aaggaacttc tgcgtatcgc gcagttcggt cgcatggaca
      181 cgaaacaata ggtcagcgtg atcggagagc ccccggagaa gatacagatc aatggccaat
      241 ttctctcgat gctgctccac aactcccttc agcacagtca gatgcgcgat gcgcgtcggc
      301 tgatcctcct tcgcccattc ttcatcaaat ccgaatgcca caaaggtccc gtacactccc
      361 ggttcactca ggagcttctc ccgatccgcc gcaccggctg cgcccggcat cgccagccac
      421 accgcagtca tgcacagcgc cgtgccgcgc gcgatcgtcg tcatcatcgt ttcttccccc
      481 ttggtccgaa cacccgtcga taaatctggt cgatgtgccg cagatagtac ttgggattga
      541 aacaggcctt gatctcgctc gccttcagat gcttggtgat gaaagagtcc ttgctcacca
      601 acgtctgtaa atccccctcg ccgttccacg aggccatcgc gtttttctga accgcttcgt
      661 acgattcctt acgttgggca cccttttcaa tgagtgcaag cagaagccgt tgcgagtaga
      721 ccaacccgcc ggttagctcg aggttccgct tcatggttgc ggggtagacc acgaggttct
      781 tgatgacatc ggtgatccgc gccagcatat agtccacgag aatcgtgctg tccggcatga
      841 tgacgcgctc aaccgacgaa tggctgatgt cacgctcatg ccacagagcc acgttttcca
      901 tcgccgcgag gctattggcg cggaccacac gcgcgaggcc gcagagattc tcggaggcga
      961 tcggattacg cttatgcggc atcgccgacg aacccttctg tccctccgtg aagtactcct
     1021 ccgcttccag cacttccgtc cgttggagat ggcgaatttc ggtcgcaaac ttctcgatgc
     1081 tcgccgcgag cagcgcgagc gccgtcgcat aggcggcatg gcgatcccgt tgaaccactt
     1141 gactcgaaac gggatcgggt ttcaggccca gcttggcgca gacatattct tcgatgtcgg
     1201 gcccttggtg cgcgaacgtc cccatcgcgc cggagagttt tcccacggcg atgtcgtcac
     1261 gcacgcgtcc gagccgctcc cgatgtcgac acacttcctc gtaccagata gccagcttga
     1321 aaccgaacga gatcggctcg ccgtgaatgc cgtgcgagcg gccgaccatc accgtgttct
     1381 tgtagcggat cgcctgccgc tggaggaccg tcagcaaacc gtcgagatct tccaaaatca
     1441 tgtcgagcgc ctcggtcatt tgaatggcca atgacgtatc gacgatgtcc gacgaggtca
     1501 gccccatgtg gagaaaccga tgctccggcc cgactgattc catgagcgac tcaaggaagg
     1561 cgatgacgtc atgtttcgta accttctcga tctccgcgat cacgtcaaca ttgatcctgg
     1621 ccttcttccg aatcttcgcc gaggttccac gcggcacctg acccgcccgt tcaaatgcgg
     1681 cgcacgcctg cagctcgacc tcaagccaga tctcatattt gtgcttgagc tcccagaggg
     1741 ccttcatctt gggacgggaa tatctatcga tcatgaagcc ctcgtgatgc gtgacgcgtg
     1801 acatgcgaag gaacagctgc ttgaaaatgg ctgactttat tctgtttcac ataacgcatg
     1861 acgccttacg cattccgtct ctccacctca cgcttcacac ccgcagcttc tgcacgcttc
     1921 acgtcgaggc gcggatcgga catcaagact ttatccagaa tgccgttcac aaatttcgag
     1981 gcttcgtcgt cgccgaaact ttttgccagc tcaatcgctt cgttcaacgt caccttcgcc
     2041 ggcacctctc ccatccagag caactcgtac agacccgccc tcaggatgtt ccggtccaca
     2101 atcggcatgc ggctgacttt ccaattcgtg gcatgttttc cgattaacgc atcgagctcc
     2161 ttcttgtgtt ccagcacacc ctgtacaatc tgctgagtaa aggccttgac ctcatcggtc
     2221 accttgacgg ttttccaaaa ctcgtcaagc cagacatccg gcttgccatg gatatcgtac
     2281 tggaacaaga tttggagggc gtgttcgcgg gcttgatggc gcgatcccat agttacgtct
     2341 tcgatcgacg ccgaaccggt cgccccctgc cattcgaggc cgccacggcc ttcgtcgatt
     2401 gcgcctgatc ggtggtcttt aacagcctca tgaccgaagc catttcgatc gcggacttgg
     2461 ccgcttcgcc cccgcgattg aacttggccg gatccgcccg ctcgatcgcc tgcgccacgg
     2521 tatccgtggt gaggacgccg aagatcacgg gcacatcggc atccaacgcc gcttgaccga
     2581 ttccccgact tgcctctgca ctgatatagt cgaagtgggg agtatcaccg cgaatgacgg
     2641 cgcccaggca gatcaccgcg tggaattgct gcgatcgcgc catggtgcgt gctacgatgg
     2701 gaatctcgaa cgcgccgggt acccggacaa cttcgatgtc gtcggccttc acaccgtgtt
     2761 tcgtcaacgc ctccgcgcag gacgacagca acttgctcgt cacgaattta ttgaacttgc
     2821 ttaccacgat gccgaaccgc aaccccgtcg catcgtgaga gcccttccgc agtttcattt
     2881 tcccgctttt tctccttggg cacgttcaca attgctaagg ggaatagcta gcttgtcctt
     2941 caattgcgcg cgtcgagcga ccacagtttt atcgcggggg ctccgcgagc acggaaggac
     3001 gggctagctg ctcctcctct ttcacacctt cttcagaatg tgccccaact tctgtttctt
     3061 ggtacgcaag tacttcacgt tggcgtcgcg cggttgaatc tcgatcggca cacgctcgac
     3121 cactttcaat ccgtaccctt caatgccgac gatcttgcgc ggattgttcg tgatcaactt
     3181 gatctggtgg agaccgagat tcgccagaat ctgcgcccct acgccgtaat cgcgcaagtc
     3241 ggctttgaac ccgagtttca aattcgcttc aaccgtatcg ctcccctggt cttgcagcga
     3301 ataggccttg atcttgttca gcagcccgat cccacgtcct tcctgattga ggtagagcag
     3361 gactccccgc ccttcctttt gaatgatctc catggcttta tgaagctgat cgcgacagtc
     3421 gcagcgcaac gatcccaacg cgtcggccgt gaggcacccg gaatggacac gcaccaaggt
     3481 cggcgctccg ctatcgaccc gacccttcac cagagcgatg tgtgtgacat tatcgatctc
     3541 gttttcaaac gccactgcct caaactcccc gaacgtggtc ggcagcgtgg cgctggccat
     3601 ccgcttgacg aaggtctcgc ggcgcatccg atattcgatc aacgccttca ccgtcaccat
     3661 tttcagcttg tgggctttgg cgaatttcgc cagttccggc acccgcgcca tcgtgccgtc
     3721 ttcgttcatg atttcgcaga tcactccggc cggctgaagc cccgccaatc gcgccagatc
     3781 caccgagcct tccgtttggc cggcgcgtcg caagaccccg ccctgctggg ccttcaaggg
     3841 gaaaatgtgt ccgggccgcg cgagatccgt gggcttggat ttcggatcga tcgcgacatg
     3901 aatggtcgtc gcacgatcgg ccgcggagat gccggtagta atgtctttcc gcgcatcaat
     3961 cgagacagtg aacgccgtac cgaacgtcgc ggtgttctcg accgcttgct gaggcaagtg
     4021 cagttcgtcg actcgctccg gcgtgagcgc gaggcagatc aatccgcggg catgtttcgc
     4081 catgaagttg atcgattgtg gcgtcacctt ctcggcagcg atgacgagat ccccttcgtt
     4141 ttcccggtct tcatcatcaa ccaggatgac gaatttcccc ttcttaatgt cccggatcgc
     4201 gtcttcaatg ctgttgaacg gcgtaggcat aatctcaatg gcgattattc aactagacga
     4261 tcaatttatc atttaacatc gaccatttaa cattcttttt ttggcccctt cctgtcatcg
     4321 gcataaaggc ccgattacga gccttcgtgg tttctgccct ccactcgtcg aatgagcaca
     4381 cccacgctg
//
LOCUS       DATDNW010000108         5839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_425745, whole genome shotgun sequence.
ACCESSION   DATDNW010000108 DATDNW010000000
VERSION     DATDNW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425745"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VI359_02750"
     CDS             complement(<1..327)
                     /locus_tag="VI359_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973203.1"
                     /translation="MAIDQAFRFSSLLLAASAFTGLVLARAVPLWLAILTGIILSLTL
                     LQTAGVTVYRDVAAETIVPSVIWNLLLITAFIGFILDLFFVSRELLPAGIHFLVILLT
                     IKLASLR"
     gene            complement(317..1444)
                     /locus_tag="VI359_02755"
     CDS             complement(317..1444)
                     /locus_tag="VI359_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY6973204.1"
                     /translation="MNVLVDQSSSLIRWLLRQLPVRRSIRLTSEGTRFLLFTLGIGVA
                     AINTGNNLFYLLLAMMLSLIVISGLMSEYSLRRLEFHRHFPDLITAHEATTVTLSVTN
                     RNVHIPSFSLHLFDVIDGNDIDRGLWIHHLPPQQSVLLSYPLIATKRGWLRLDGLRVQ
                     TLFPFGLFLKRARFLTPSKLLVGPQIKPMAVRFVDELVSQGQGYSVPRRGHGTELHNL
                     RLYQPGDDSRTIHWMSTARTSQLIVRETEAEDQRRLTVVLSTVAPDHSDVLFERAVTL
                     VASLLWELSQRSYAIRLVVGTADSGFGSGSTHFGTILHLLALCERRNPTDEKTPFYFH
                     GGEEGYLLAVIPWADPVAVSAMADADRLLDETQLEELTHGY"
     gene            complement(1476..2417)
                     /locus_tag="VI359_02760"
     CDS             complement(1476..2417)
                     /locus_tag="VI359_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY6973205.1"
                     /translation="MTSVHRIRAIQENIARVIKGKPRVIEMAVVCLLARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQKQGFEFMPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQISVDNQTYPLRQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPPAEEERKVLDRPHSLHPAEDLQPVAAVPDVLELQARVEQVHIEE
                     SLMDYLLAIVHATRQSEFLALGVSTRGAVALSTAAKALALVRGRDYCLPEDVKELAPI
                     ILSHRIMVNRTHGMRSQNFEQAERIVSDIVDTVPVPV"
     gene            complement(2581..3900)
                     /locus_tag="VI359_02765"
     CDS             complement(2581..3900)
                     /locus_tag="VI359_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973206.1"
                     /translation="MSSHAQVLADFRSQITKLLEERDVQWQASRRLVGANCLGTTLKR
                     LLEEARRVEIPIPVRDAIEIALKRGEVASLQDLPGPRLKELTGLPPTKAVRALCVWFD
                     LVESPASQWPLSLLSNETVATFIQEHDNPFDLLLDADVASLLDIGSGDLSFATELADQ
                     YMPRFQQQSRQLTLHCLDRLHPDSKLGGPLHPERQRLDALRTRPGLSFQFFGNQDMFA
                     LEELERTGRMAPRYTVVTCWAPATPTFAYEPTRLSVDVITAELGRTKGAFRQTRFEGE
                     AALEVQHGDRTLLFPPWKFEIRGPLALLDLMSRLGVLCILGAVDSQVFWELLAQLLED
                     ERYRPQDLPFTTQTIPNVFGEIFGRLESLDVGHRLDLTDVGPIRAQLPRVLPVRSGER
                     GHYRFSSIQIRRGAVFPNIPASRTARQFPDMAEETTPWFLCLVPERP"
     gene            4115..5050
                     /locus_tag="VI359_02770"
     CDS             4115..5050
                     /locus_tag="VI359_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY6973207.1"
                     /translation="MSITACVSGDPVSSQVIQNEERFHDWQVIDTVSGQAVRMDDVMR
                     ELALQDVVYLGEEHHNRYHIEAALQVLRSLIERGRKPTLAMEMFGWDGQQAIDRYVSG
                     QESDRSTFIAQSRWLQNWGGPFEDYEPLVRFVKDRHLSLVALNPPKSLVRMVMKQGLV
                     AARSDPEMAQWGMKDESIVDDPLYRERIIRQLQQCHGGGESLYQSMYEASMVRDEGMA
                     KTVVERLAASRSAEGPAAGPLVSYTGGGHIQYQLPVPKRVIRRAGLHVRQRSIYMTSF
                     DRDRASELEEMIRDKIADYIWLTPIGAQGPPRRCR"
     gene            5139..5336
                     /locus_tag="VI359_02775"
     CDS             5139..5336
                     /locus_tag="VI359_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY6973208.1"
                     /translation="MEVGPCTIYWCRCGRSKTQPFCDGSHTGTDFAPIEVHFEEKKRV
                     ALCGCKHTKKPPFCDGTHSRI"
     gene            5535..>5839
                     /locus_tag="VI359_02780"
     CDS             5535..>5839
                     /locus_tag="VI359_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973209.1"
                     /translation="MRNKIPCSAERLSLLDPPMIQSVVDRGAFQIGTQYVAENRVRIV
                     EADESQISSAVIGSSGLYEQTIRLKDGHLVSKCSCALPEEPMCRHCIAVLLEYHRWA"
BASE COUNT         1365 a         1493 c         1760 g         1221 t
ORIGIN      
        1 gcggagcgag gccagcttga tggtaagcag aatcaccaga aagtggatgc ctgccgggag
       61 aagttcccgc gagacgaaga agagatccag tataaaaccg atgaatgccg tgattaagag
      121 gagattccag atcaccgatg gcacgatggt ctctgccgcc acgtctcgat ataccgtcac
      181 ccccgcagtc tgcagcaggg tgaggctcaa gatgatgcct gtcagaatgg cgagccacag
      241 cgggactgcg cgcgccagca ccagaccagt aaaggctgag gcagccagca acagggaact
      301 gaatcggaag gcttggtcaa tagccatggg tgagctcttc aagctgtgtt tcatcaagca
      361 gtcgatctgc atcagccatc gccgagactg ctacagggtc cgcccatggg atgacagcca
      421 acagatatcc ctcttcgccg ccatgaaaat aaaacggcgt tttctcatca gtcggatttc
      481 ggcgctcaca cagagccaat aagtgcagga tggttccgaa gtgcgtagag cctgatccaa
      541 aaccggagtc tgctgtcccg accacaagac gaatggcata agatcgttga gacaattccc
      601 agagaagaga tgccaccaag gtgacggctc gttcgaacaa gacgtcactg tgatctggcg
      661 ccacggtcga cagcacgacc gtgaggcgcc gctgatcctc ggcttcggtc tcgcggacaa
      721 tcagttgtga tgtgcgcgcg gtagacatcc agtgaatggt ccgagagtcg tctccgggtt
      781 ggtagagacg aagattgtga agctcagttc catgcccgcg ccgcgggacc gaatacccct
      841 gtccttgcga aaccagttcg tccacgaatc ggacggccat gggtttgatc tgaggcccga
      901 cgagaagctt cgacggcgtc agaaagcgcg cccgcttgag aaagagacca aacggaaaca
      961 aggtctgaac tcgaaggccg tcgagcctga gccaacctcg tttcgtagca atgagcggat
     1021 acgagagtag gactgattgc tgtggaggaa ggtggtgaat ccacagaccg cgatcgatgt
     1081 cgttgccgtc gataacgtca aacagatgga gggaaaaact ggggatgtga acattgcgat
     1141 tggtcaccga gagagtaacc gtcgtcgcct catgggccgt gatcaggtcg ggaaaatgtc
     1201 gatggaattc caggcggcga aggctgtact cggacatgag cccggaaatt acgatgaggc
     1261 tcaacatcat cgccagtagc aggtagaaga ggttgttgcc ggtattgatg gcggcgaccc
     1321 cgattccaag agtgaagaga aggaagcgcg tcccttctga cgtgaggcga atcgagcggc
     1381 gaacgggcag ctggcgcagg agccagcgaa tgagtgagga tgactgatcc accaatacgt
     1441 tcatggtggg atgttccatt aaagagtagc ggtgcttaaa cgggaacggg aaccgtgtcc
     1501 acgatgtccg acacgatccg ttcagcttgc tcgaaatttt gcgaccgcat gccgtgggtt
     1561 cgattcacca tgatgcggtg ggagaggata atgggggcca actccttcac gtcctctggg
     1621 agacaatagt ctcggccacg gacgagcgcc aaggccttgg cggccgtgct gagcgctacc
     1681 gcgccccttg tgctgacgcc caatgctaga aattcgctct gacgggtggc gtgcacgatg
     1741 gcaagaagat agtccatcag gctctcttca atgtgaacct gttcgacgcg ggcttggagt
     1801 tccaaaacat cgggaactgc cgccacgggt tgtaaatctt ctgccggatg gagcgaatgc
     1861 ggacggtcga gcactttgcg ttcctcctct gccggaggat agccgatgcg caggcgcatc
     1921 aggaatcggt cgagttgcga ttccggcagg gggaacgtcc cgtggtattc ggcagggttt
     1981 tgggtcgcaa tgaccataaa cggctggcgc aagggatagg tctggttgtc cacggaaatt
     2041 tgcgcctcgc tcatcgcctc caaaagactg ctttgcgtct tcggcgtcgt tcgattgatc
     2101 tcatcggcca agacgatgtt tgcgaagatg ggacccggca tgaattcgaa cccctgcttt
     2161 tgtcgattga agattgacac gccaacgata tctgacggaa ggaggtcgct ggtgaactga
     2221 atgcgtttga aggaacaatc gagtgaacgc gccagactat gcgcgagcgt ggtcttgccg
     2281 acaccgggaa cgtcttccag aagcaaatga ccccgtgcga gaaggcagac caccgccatt
     2341 tctatgacac gcggcttccc cttgatgacg cgcgcgatat tctcctggat ggcccggatt
     2401 ctgtgcacag atgtcatggt gaatttatgg gttgtggtga ttgaggaatg gtatgtggat
     2461 ggacacggtc gaagtctaac aaagggtcga tatacggtca atttttccgc ggttttctgt
     2521 cgtcatcgtg atccggattt gtcgcggtac agtcgaacaa aaccgtcggg tagttccgac
     2581 tcacggacgt tctggaacga ggcaaaggaa ccagggggtc gtctcctccg ccatatcggg
     2641 gaattggcga gccgtcctac tggctggaat attagggaag accgctccgc gacgaatctg
     2701 gattgaggag aagcggtagt gtcctctctc tcctgatcgg accggcaaca cacgtggaag
     2761 ctgtgctcgg attggtccca catcggtcag atccagtctg tggccaacgt cgaggctctc
     2821 cagtcgaccg aagatctcgc cgaatacgtt cggaattgtc tgcgtggtga agggaagatc
     2881 ttgtggtcga taccgctcat cttcaaggag ttgagcgagg agttcccaaa acacttggct
     2941 gtcaacggct cccaggatac acaacacgcc caggcgggac ataagatcca gcaatgcgag
     3001 cgggcctcgg atctcaaatt tccatggtgg aaacaacaga gttcggtcgc cgtgttgaac
     3061 ctcaagcgcc gcctcgcctt cgaagcgtgt ttgacggaac gcgccttttg tgcgaccgag
     3121 ttcagccgtg atcacatcaa cagacaatct ggttggttca taggcaaacg tgggcgtggc
     3181 gggtgcccaa caggtgacga cggtatagcg cggcgccatc cgtcctgtac gctccagttc
     3241 ctctagtgca aacatgtctt ggttgccgaa gaattgaaac gagaggccgg gtcttgtccg
     3301 aagcgcgtcc agtcgctggc gttcgggatg gagaggcccg ccgagtttcg aatcagggtg
     3361 aagtcgatcg agacaatgga gcgtcaattg ccgactttgt tgctggaagc gcggcatgta
     3421 ttgatcggca agttcagtgg cgaacgaaag gtcccccgat ccgatgtcca acagcgaggc
     3481 cacatctgcg tcgagcaata aatcgaacgg attgtcatgc tcttggatga acgtggctac
     3541 cgtttcattt gacagcaaag acagaggcca ttgcgatgcc gggctttcga ccaggtcaaa
     3601 ccagacgcag agtgcacgaa cggctttagt cggagggagc ccggtcagtt ctttgagtcg
     3661 aggaccaggc agatcttgca ggctagccac ctcgccacgc ttcagcgcga tttcaatggc
     3721 gtcgcggacg ggaattggaa tctccacgcg gcgtgcctct tcaagcagcc gtttgagggt
     3781 ggtgccgaga caattggctc cgacaaggcg tcgcgaagcc tgccattgta catctcgctc
     3841 ttccaggagc ttggtgattt gcgatcgaaa gtctgccagg acttgggcgt gagagctcat
     3901 gctaccgtga aatgcgcaag agatgcataa cgcttccttc tggttgacca ggagcgcgag
     3961 gcagcataac gggccgatct cagagcgtca aggttgtccg cttgctggtc caagggagct
     4021 atgttaggta aggcatatgt ctcgctctcg gtttgaacga cgccgttcct tcgatcgcct
     4081 tcgcagatgt tctgcgctgc tagggatggc cctcatgagc atcacggctt gcgttagcgg
     4141 cgacccggta tcttcccaag taatccagaa cgaagagcga ttccatgatt ggcaggtcat
     4201 cgacaccgtc agcggccagg cggttcgtat ggacgatgta atgagagaac tggctctgca
     4261 ggacgtcgtc tatctgggcg aggaacatca caaccggtac cacatcgagg cggctctcca
     4321 agtattgcgg agcttgattg aacgcggacg taaaccaacg ctggccatgg aaatgttcgg
     4381 ctgggacggt caacaggcga ttgatcggta tgtctcaggg caggagagcg accgatccac
     4441 attcatcgca caaagtcgct ggcttcagaa ttggggcggc ccattcgaag actacgagcc
     4501 cctggttcgg ttcgtgaagg atcggcacct ctcgctcgtg gcgctcaatc cccccaaaag
     4561 cttggtgaga atggtcatga agcaaggatt ggtggcagcg aggtctgatc cggagatggc
     4621 ccaatggggt atgaaggatg agtcgattgt agatgacccg ttatatcgcg aacggattat
     4681 ccgacaactc caacaatgtc atgggggagg ggagagcctc tatcagtcga tgtatgaggc
     4741 ctcaatggtt cgggacgaag gcatggccaa aacagtcgtg gaacggctcg cagccagtcg
     4801 atctgctgaa ggtcctgcgg caggaccact ggtgagctac acaggagggg ggcacattca
     4861 ataccaattg ccggtgccga agcgagtgat ccgtcgagcc gggttgcatg tccgccaacg
     4921 cagtatctac atgacgtcat ttgatagaga ccgggcctca gaattagagg agatgatacg
     4981 ggacaagatt gcagattata tatggctgac acctatcgga gcacaaggac cgccgcgccg
     5041 ttgtcgatag gttagatgaa tgagacaatc ggcacgagaa ataatgagga ggtagcatgg
     5101 ctgatcagcc cgtcatcgca cgacgcagtc cttatgtaat ggaagttggg ccgtgcacga
     5161 tctattggtg tcgttgcggt cgatccaaga cacaaccgtt ctgtgacggg tcccatacag
     5221 gaaccgattt cgctccgatt gaggtccatt ttgaagaaaa gaaacgggtg gcgctctgtg
     5281 gctgtaaaca cacgaagaag ccgccgtttt gtgatggtac ccactcgaga atttaagacc
     5341 aatggacgac gatttgcgac atcctgcctg tgaaacacct ccatggttct gctcgccgct
     5401 gtcttagcct cttgcccgcc tctcggtcag gtatgcaact gccggaacca ggcgaacgct
     5461 tgacagttga atcagtccgt tccagactac cccgaaatat cacacgggtc atttcaaact
     5521 tgggaattca acggatgcga aacaagatcc cctgctcagc ggagcgactc agtcttctcg
     5581 acccccctat gattcagtcg gttgtggatc ggggtgcctt tcaaatcggg acccagtatg
     5641 ttgcggaaaa tcgggtccgc atcgtcgaag cggatgaatc ccagatttcc tcggctgtca
     5701 ttggcagctc aggattgtac gaacaaacga tcaggctgaa ggatgggcac ctggtgtcga
     5761 agtgcagttg cgctctgccg gaagaaccca tgtgccgcca ttgcatcgct gtattgctgg
     5821 aatatcatcg atgggctca
//
LOCUS       DATDNW010000109         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1172801, whole genome shotgun sequence.
ACCESSION   DATDNW010000109 DATDNW010000000
VERSION     DATDNW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172801"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VI359_02785"
     CDS             <1..355
                     /locus_tag="VI359_02785"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY6973210.1"
                     /translation="NHAGGILGGISTGQNVVVTIGIKPTSSVRLPRRSIDKQGNPVTV
                     ETNGRHDPCVGIRATPIAEAMMALVLMDHALLHRAQNADVTTPTPKIAGSPKTSSHAK
                     STAGPAKINPMPDEA"
     gene            345..1142
                     /locus_tag="VI359_02790"
     CDS             345..1142
                     /locus_tag="VI359_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6973211.1"
                     /translation="MKPSPFIEIERATVFRGDVCALQDFSLTVESGEHLAILGPNGAG
                     KSTFLKLLAGEIHPVPNDTTRIRLFGDERWNVWDVRKQLGFVSHDLQHQYMDHVLGIK
                     VVLSGYYASIGIYGYQDFGYAQLARASEVMDELGVGHLKERRFADMSTGEQRRCLLAR
                     ALVHDPTVLVLDEPTSGLDLSACFQYLDLVRTSMRQDKTVLLVTHHIHEIPPEVERVM
                     MLRAGVVLRDGTKQDILTDANMSQLFSHPMMLVRAHGWYQALPAAPT"
     gene            complement(1154..2143)
                     /locus_tag="VI359_02795"
     CDS             complement(1154..2143)
                     /locus_tag="VI359_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6973212.1"
                     /translation="MVGAIALASTLAQPGWGESSSSVLKEMFERAKRATVGILEDTQD
                     PRTPEKPGKIAVRGTGVHLRDGYVVTARHAVERNTPSGPILPTHIRLITTDLHELPAQ
                     LVGESAYLDVVVYRIAEKNRHLMTGTAPFAAGNVEPGTEVFTIGYPMGWGPTMAFGRI
                     GNANTFLQTVDTRLLQADLSACSGNSGGGLFNMAGEIVGVMHAIIQTDKDETQVHCSQ
                     MAFAVPGTLAQRIVAAAIDGKPLGFSKLGVHMTAVKDGTRWRIAVKDVTDPAKSAGIQ
                     KHDIILSVDDTEILDAAHLKNYLIERTTPGQRVTVKVRRVDADLTFTVVLGSG"
BASE COUNT          468 a          662 c          698 g          520 t
ORIGIN      
        1 caaccacgcc ggcggcattc tcggcggaat ttcaaccgga cagaacgtcg tggtcacaat
       61 cggcatcaag ccaacctcga gtgtccgtct cccgcgccga tcgatcgata aacagggtaa
      121 tccggtcacc gtcgagacga atggccgcca tgatccgtgc gtcggtatcc gggccacccc
      181 tatcgccgaa gcgatgatgg cgctggtcct catggaccat gctttgctgc atcgtgcaca
      241 aaatgccgat gtgacaacac ccaccccgaa gattgcgggc tcccccaaga cgagctcgca
      301 tgccaagagc accgccggcc cagctaagat caatccgatg ccggatgaag cctagtcctt
      361 ttattgagat tgagcgcgca acggtgtttc gaggcgatgt ctgcgcgctt caggattttt
      421 ccctcaccgt tgagtcggga gaacatcttg ccattcttgg acccaacggc gccggcaagt
      481 cgactttctt aaagttgttg gccggtgaaa tccatccggt tccgaacgac acgacccgta
      541 tccggctgtt tggggacgaa cgttggaatg tgtgggacgt gcggaagcaa cttggattcg
      601 tctcgcacga tctgcagcat cagtatatgg atcatgtgct tgggatcaag gtggtcctct
      661 ccggttacta tgccagcatc gggatctatg gctatcagga cttcggttac gcgcagctgg
      721 cgcgtgccag tgaggtcatg gacgaactcg gcgtgggaca cctcaaagag cgacggtttg
      781 ccgacatgtc gaccggcgaa cagcgccgat gcttgctcgc tcgggcgctg gtgcatgatc
      841 caaccgtgct ggtcctcgac gaaccgacga gcggtctcga tctcagcgcc tgctttcagt
      901 accttgacct ggtccgaacg tcgatgagac aagacaaaac cgtcctgctg gtgacacatc
      961 atatccacga gattccaccg gaggtcgagc gggtgatgat gctacgagca ggagtcgtcc
     1021 tgcgcgatgg gacgaagcag gatatcttga cggatgccaa catgtctcag ttgtttagtc
     1081 atcccatgat gcttgtccgt gcgcatggct ggtatcaggc attgcctgct gcgccgacct
     1141 aaacgcgggg aggtcacccg ctgccgagca cgacggtgaa ggtcagatca gcgtcgaccc
     1201 ggcggacctt gacggtcaca cgctggcccg gtgtggttcg ttcgatcagg taatttttca
     1261 ggtgtgcggc gtcaagaatc tccgtgtcat cgacggaaag aataatatcg tgtttttgaa
     1321 tcccggcgga tttggccggg tccgtcacgt ccttcacggc gatgcgccat ctcgtgccgt
     1381 ccttgacggc ggtcatgtga actccgagtt tcgagaagcc gagcggtttc ccgtcgatcg
     1441 cagctgcgac gatccgctgg gcgagtgtgc cggggacggc aaaggccatt tggctgcagt
     1501 ggacctgcgt ctcgtctttg tccgtctgga taatcgcgtg catcactccg acgatttcgc
     1561 ccgccatgtt gaacagtccg ccgcccgagt ttccactgca ggccgagagg tcggcttgca
     1621 ggagcctggt atcgactgtt tgcaagaacg tatttgcgtt gccgatgcgt ccgaaggcca
     1681 tcgtgggtcc ccagcccatc gggtatccga tcgtgaacac ctccgtgcct ggttcgacgt
     1741 tgcctgcggc aaacggggcc gtgcctgtca tcaaatgccg gttcttttca gcgatgcggt
     1801 agacgacgac atcaagatac gcgctttcgc cgacgagctg ggccggtaat tcatgcaggt
     1861 ccgtcgtgat caggcggatg tgcgtcggga ggattggacc cgacggggtg ttccgttcaa
     1921 ccgcatgacg tgccgtcacc acataaccgt ctcgcaggtg aacaccggtc ccgcggacgg
     1981 caattttccc tggcttctca ggagtccttg ggtcctgggt gtcttccaag atgccgactg
     2041 ttgcccgctt ggcccgctca aacatctctt tcagtactga ggaggaagat tcgccccatc
     2101 ccggttgtgc gagcgttgac gcaagcgcga tagcccccac cacgatcctc aagatgaaga
     2161 ttggaagatg ttgcctacgt ggagcgatca aagacaccac gatcacgtcc tttcgattaa
     2221 gcgtggagat gatgacagta tagtcagttt catggagcga ggaaactggt ttggtttcgt
     2281 ggtatcgtaa aattaatgaa actcgggagg gccttagtgg ctgcgaaatg gaacgtgctc
     2341 gccggtat
//
LOCUS       DATDNW010000110         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_78938,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000110 DATDNW010000000
VERSION     DATDNW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78938"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="gspK"
                     /locus_tag="VI359_02800"
     CDS             complement(<1..773)
                     /gene="gspK"
                     /locus_tag="VI359_02800"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY6973213.1"
                     /translation="MQKHDERGIALLLALLVLALLVAIILEFDAEARREYRDAAAFRD
                     NFKASVLARAAVQAARAVLQQDFLRDKQAGQQFDGPTDLWAFPIKNYAIGDGLLNAQI
                     EDERGKLNLNDLAAGGDPNAKKAVALRTKRLFELLQVNPDLVDVISDWVDADEVPGAA
                     GAETLYYQSLRPAYRSANAPLNTLLELRLVKGMTPEIIDRLSKFVTVYPPDGGSKVNL
                     NTADPIVIQALDPRITQTVAGEIVQGRPFKTIQDLDRVS"
     gene            complement(760..1308)
                     /gene="gspM"
                     /locus_tag="VI359_02805"
     CDS             complement(760..1308)
                     /gene="gspM"
                     /locus_tag="VI359_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY6973214.1"
                     /translation="MIQTLTERWQHYSPRERVLLFIGGAVVAASLVFLVIVDPLLSKL
                     DKLDRQLNRKQKDIQELAIVAQAYAGKRTTLTKLEQRMPPADAQFSLLAFMEEATTTA
                     QIRDRIVGMQPQNPTVLQGYQETAVDLRLDGVQLPQLLALLVAIEQAPYEVQVRHLQL
                     KPKFDNPTNLDATLRIVTYAKA"
     gene            complement(1305..>2179)
                     /locus_tag="VI359_02810"
     CDS             complement(1305..>2179)
                     /locus_tag="VI359_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973215.1"
                     /translation="EAERRKRVMAVQEVDAWLEPLLKELRVTLHPYEGTGRQGITHCW
                     VSGGGSKLRELGGHIAHNLGLVPVGPRQGFGASCPRAFSIAFGLAIHPKIIRSRWRGR
                     SADTDLAVDLKEASALGLEPSSTAKRDRRLAIWGALVIGLLALFDLSMRFVLKDSAFT
                     QLKQSLQIQYEQTFGAGAAPGEELDQARYRLSQVEKSLSVIDGSKISVLAMLAELGKQ
                     VPPGIPLKIRELTIDGTMVHLEGETVSFDAVEKLKQTFMSAAHVHDVTVTDTRVGAVP
                     NQVVFRLNYSVKRP"
BASE COUNT          462 a          616 c          592 g          509 t
ORIGIN      
        1 ctgctcacac gatcaaggtc ttggatggtc ttgaacggtc gtccttgcac gatctcaccc
       61 gccacggttt gtgtaatccg tggatcgagt gcttggatca cgatcggatc ggccgtattc
      121 agattcacct tacttcctcc gtcaggaggg tagacggtaa cgaatttcga cagccggtcg
      181 atgatctccg gcgtcatccc cttgactaag cgaagttcca acagagtatt gaggggggca
      241 tttgccgagc gatacgcagg acgtagtgac tgatagtaga gggtttcagc ccctgcggct
      301 ccgggcactt cgtctgcatc gacccaatca ctgatcacgt ccacaaggtc tgggttcact
      361 tgtagcaact cgaacagccg tttcgtacga agggcgactg cttttttcgc gttaggatcc
      421 ccgccggcag ccagatcgtt aaggttcagc ttccctcgtt catcctcgat ttgagcgttc
      481 aggagtccat ctccgatcgc atagttcttg atgggaaagg cccagaggtc tgtcgggcca
      541 tcgaactgct gacccgcttg tttatcgcgc aaaaaatcct gttggagtac agcccgtgcc
      601 gcctgcaccg cggctcgtgc caatacggag gctttgaaat tatcacgaaa ggcggccgcg
      661 tcgcgatatt ctcgacgcgc ctcggcatcg aattcgagaa tgatggctac caacagcgcg
      721 aggaccaaaa gagcgagcag gagcgcgatg ccccgttcgt catgcttttg cataggtgac
      781 gattcgcaat gtggcatcga gattcgtcgg attgtcgaat ttaggcttga gttgcaggtg
      841 acgcacttgg acttcgtacg gcgcttgctc gatcgcaacc aacagggcaa ggagttgcgg
      901 caactgcaca ccgtccagac gcagatcgac ggcggtttcc tggtatcctt ggagcacggt
      961 cgggttttgc ggttgcatgc caacaatgcg gtcacggatc tgtgcggtgg tcgttgcttc
     1021 ttccataaac gcgaggagcg agaattgagc atcggccgga ggcatccgct gttccaactt
     1081 tgtcagcgtg gtgcgcttcc ccgcataggc ctgagctaca atggccagtt cttggatgtc
     1141 tttttgcttg cgatttaact gccgatccaa cttgtcgagt tttgacagga gagggtccac
     1201 gatcacgagg aagacaaggc tggccgcgac aaccgctccc ccgataaaga ggagcacccg
     1261 ttcccgaggg gaatagtgct gccatcgttc tgtgagcgtc tggatcatgg ccgtttgacc
     1321 gaatagttca aacgaaaaac gacttgattc ggtaccgcac ccactcttgt gtcggtgacg
     1381 gtcacatcgt ggacatgggc tgccgacata aacgtctgct ttagtttctc cactgcatca
     1441 aatgatacgg tttctccctc gagatggacc atcgttccat cgattgtcag ttcacgaatt
     1501 ttgagtggta ttcccggcgg aacctgcttt cccaattcag ccagcatcgc gaggactgag
     1561 atcttcgatc catcgatcac cgacaaactc ttctcaacct gcgagaggcg atagcgtgcc
     1621 tgatcgagtt cttcgcccgg agccgctccc gcaccaaacg tctgctcata ttgaatctgt
     1681 agcgactgct tcagttgcgt gaacgccgaa tctttcagca caaagcgcat ggagagatca
     1741 aacaatgcca acagtccaat cacgagcgcg ccccagattg ccaatcggcg gtcccgtttc
     1801 gccgtactgg atggctccag gccaagtgcg cttgcttcct tgagatcgac cgccaaatct
     1861 gtatcggcag atcggcctct ccaacgagag cgaataattt tcgggtgaat ggctaatcca
     1921 aaggcaatgg aaaacgcacg tgggcaactt gcgccgaatc cttgacgcgg cccgactggg
     1981 accaatccca agttgtgtgc gatgtggcca cccagttccc ggagttttga cccgcctccc
     2041 gaaacccagc aatgtgtgat tccttgtcgg ccggtacctt catagggatg cagcgtcacc
     2101 cgcaattcct tcaacagggg ttcgagccag gcatcgacct cttgcactgc cataacgcgc
     2161 ttgcgccgtt cggcctctg
//
LOCUS       DATDNW010000111         6126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1240820, whole genome shotgun sequence.
ACCESSION   DATDNW010000111 DATDNW010000000
VERSION     DATDNW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240820"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(636..1157)
                     /locus_tag="VI359_02815"
     CDS             complement(636..1157)
                     /locus_tag="VI359_02815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6973216.1"
                     /translation="MTMEFKKIDPRVTITTKFGEIKIRFYPEDAPRHLDNFLKLAKMG
                     FYDGTTFHRVVPGFIIQGGDPLSKEPDRRRHGSGGPGYWLNPEPSDRPHKRGAVSMAK
                     TPRDGTSTRDFNDNGSQFFICLADNSGLDRRYSIFGEVFRGMDVVDKIAAAPRDEFDN
                     PVEPIEMKMTVKE"
     gene            complement(1215..2504)
                     /locus_tag="VI359_02820"
     CDS             complement(1215..2504)
                     /locus_tag="VI359_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6973217.1"
                     /translation="MTTTTASPTKIRWIILALLFLVSMVTYIDRVNISVTARQMMPAY
                     GLTDQDMGVIFSSFVIGYALFQIPGGWLADRWGTRAVLTLALVWWSLCTALTAIAATT
                     SLVAWFGLVGALAIVRGSLGVGEAVALPAFNRAVADWCPTDSRGLGIGIAIGGIGLGS
                     AITPPLAAWIMVNWRWQTVFYLASLLGLGMALLWWIVARNHPQEHPWTNEAERAAITQ
                     VTASGSPPAIPWGMFAQTPTIWWLVASYACLGYVAYIYMSWFYLYLVNVRGFDLLRGG
                     LYAAAPFLAMLVFCPLGGWMTDRLATRCGVRSGRAWAGMTGMGFAGLAVGIGGYAESP
                     WLAVASLSLGAGWLYFAVGAYWASTTDLSKPYAGTLAGLMNTGANLGGALSPTVTPWL
                     ADRWGWPVSLLVAALIALAGALMWLKIDPGKGLMAKG"
     gene            2698..6051
                     /locus_tag="VI359_02825"
     CDS             2698..6051
                     /locus_tag="VI359_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEY6973218.1"
                     /translation="MSRPSVLVILFAVLLIAGVSFLLFSPFLTGDDYLKQFFLEQLEQ
                     NLGRKIDVHRIKFVLFPNIRLELTQVGIHDRQSDQVVLAAKKVDLVLRLLPLLRKQVV
                     GKRLVIEEPTLTLRRDRNGHWNVLDQVVQGAATDQDALQMMSRMFRIREATLVNGTVT
                     IVDEARPDGPRSMKLESVEAALKIWAERGQADVHVSAVRAGEAGVSAVSLAGRIARVE
                     QPSLTTNAPSQLVLQFDGSMEAANLNLRDVADFFGPRPIPEQLKGTINLSSTVRAQPG
                     VAGYDVLLSDLSAKAGQFALSGKASLSGLMSAQPTVALTFASAPIRLNELLTVIPAQW
                     IHAQLAAVIQDRQINGQIEAVSATVTASSVSGPQLSVTGEFHVRDAEALVGEDRVPAK
                     DVSAVVSVEAGRVRVSNLTGVYGAILMSDGKALVSFLEAGPWLELEITGTMPAADLLQ
                     FLSKTVKSERLSRVLASSREVEGQAAPTFRMVGPLNQPGGVTFAGGEITAQQISLTNP
                     SLPERLTALQGRFVLLDGETRFDQVKGHLGDLVVEVQGGMTGGSASSFQDFLVRVRGD
                     AIHMTRLLPPKSLPEGAIDGIINGDVVLTGLTDAPRLRGEVMLTEAKVFLPGMIEKPV
                     GAPASVQFDGELSTTSVLTLTRVELQLPSLRLPIKGRLQLGERFSIDAALATGTLSLS
                     SLPEWIAKGGFEAGNIELSLDMKGKDRDWRTWRTTGWLALTNGLMTASGVDGPIQDLY
                     LRLHLVRSAAELKRLSFRVADSDVSLEGTIRNWTVKPIVAARIESNNMDIDLLIPKGA
                     RSPIRDFLETLAATSKVNATATIARGHYKHLKFGSLSARITIQDGVLDIDRLSGQSPD
                     GELAGRFVVQLPRNQPADGEVSLRATGLVVEDLLKLVGSKGPVTGEARVSGTIRGHGR
                     NPHGIYPTLNGKVDVLVQDGRIFKSQDRAIWKIISILNLPAVLQGKVDLEKEGLPYSK
                     ITATVTVRDGLFETENLIIDSPIIKVTAAGNYDLPTDQLDMVWAVSPFGSYSQFLKTI
                     PLFGRLFAGDRKGFATAMFSVKGAIEDPETTYLPMKSFAVGLTGLGQLAFDLLKNTVT
                     LPLDLMTPNEDKTTDKEGQLTLEPAPSAP"
BASE COUNT         1336 a         1723 c         1793 g         1274 t
ORIGIN      
        1 gtgcagacga ttctgccctt gaaaggcggc gcaggattga gattgacgac agcgaggtca
       61 tttagtccta agggccgatc attagaggat acaattctac cggatcttat agtttcagaa
      121 gagcaaggga ttgatgctcc gttgtcatcc gccttgtcag aacttagaac cacgattccc
      181 aattgagatc cggcgagagc caaacacacg gaacggatag ttctcacata caatctggtc
      241 gtctgtgctc gttttgtgct caagcccacc tcactccagc ctattccagc ctactccagc
      301 caaagttcag gttttgtgca aaccttggct ggagtgggcc tgagtgagcc tgagtgggct
      361 ggaggagggt cagggaaacg gtttcctgaa ccgcgagttg ggatctagag agatcgaacg
      421 agacatcaag cggccggtcc gataatcctc cctgtgccga tcgaccgagc accgcaccgt
      481 aaattccatc gaagcatgct cgtctacctc ttcagcgggt ggcctggttg atcctcgaat
      541 gcgcgcgtcg aatgaccacg tcgttctcct ccagtggaaa tttgattggc agacgtgggg
      601 gttccgcgag caggaggacg atcaggccac ccggcctatt ccttcaccgt catcttcatc
      661 tcgattggtt cgacagggtt atcgaattca tcgcgcgggg ccgcggcaat cttgtcgact
      721 acatccatcc cgcggaatac ctctccgaat atgctatagc gtcgatccaa gccgctgttg
      781 tctgccagac aaataaaaaa ttgcgaaccg ttgtcgttga aatcgcgcgt gcttgtgcca
      841 tcccggggcg tcttcgccat ggacaccgca ccgcgtttgt gcggacggtc actcggttcc
      901 gggttcagcc agtaaccggg tccccctgac ccatgccttc gtcgatccgg ttctttgctc
      961 aagggatcgc caccttgaat aatgaaacca gggacgacgc ggtgaaaggt cgtgccgtcg
     1021 taaaacccca tcttggcgag tttgaggaaa ttatccaagt ggcgcggggc gtcttcggga
     1081 taaaatcgaa tcttgatctc gccgaacttg gtcgtgatgg tgacacgcgg gtcgattttc
     1141 ttaaattcca tcgtcatggc gatgaatgta ccaagggcgc ggggcgagag gcaatgggcg
     1201 agaggcgatc tcctctagcc cttagccatt aaccccttgc cgggatcaat ttttagccac
     1261 atcagcgctc ctgccagtgc aatgagcgca gccacaagaa gcgacaccgg ccatccccag
     1321 cgatcggcga gccagggggt cactgttggg gaaagggcac cgcccaggtt ggcgcctgta
     1381 ttcatcagtc ccgctaacgt accagcgtat ggtttcgaca ggtcagtcgt tgaagcccaa
     1441 taagcaccga cggcgaaata caaccagcct gcgcccaacg agagactggc aacggccagc
     1501 cagggtgact cggcatagcc gccgattccg acggccagtc cggcgaagcc catgccggtc
     1561 atgcctgccc aggcgcggcc actcctgaca ccgcaccgtg tggccagtcg atcagtcatc
     1621 catccgccga gtggacagaa aacgagcatg gccaggaatg gcgctgccgc atagagtccg
     1681 ccacggagca gatcgaagcc tcgcacattc acgagataca gatagaacca tgacatgtag
     1741 atgtatgcga catacccaag acaggcatag ctcgcgacaa gccaccaaat cgtaggggtc
     1801 tgtgcaaaca ttccccaggg tatggcagga ggtgatcctg atgcagtgac ttgtgtgatc
     1861 gctgcccgtt ccgcttcgtt cgtccaggga tgctcctgcg gatgatttct cgcgacaatc
     1921 caccagagca atgccatacc gagtcccaac agtgaggcga gatagaagac cgtctgccac
     1981 cgccagttca ccatgatcca ggcggcgagt ggtggcgtga tggctgatcc aagcccgatg
     2041 ccgccgatcg cgatgccgat cccgagaccg cgcgaatcgg tcggacacca gtcggccact
     2101 gcgcgattga aggcaggaag cgcgaccgct tcaccgacac ccaagctgcc tcggacgatc
     2161 gccagcgcac ccacaaggcc gaaccaggcg acgagggacg tggtggcggc tatggcggtc
     2221 agtgcggtac agagcgacca ccagaccagc gcgagcgtga ggaccgccct ggttccccat
     2281 cgatccgcca accacccgcc gggaatctga aagagggcat acccgatcac gaacgaggaa
     2341 aaaatcactc ccatatcttg gtcggtaagc ccgtaagccg gcatcatctg ccttgccgtt
     2401 acagagatgt tcacgcgatc gatataggtc accatactca cgaggaacaa gagtgcaagg
     2461 atgatccagc ggatcttcgt aggagaagcg gtagtggtag tcatcacgat ggatcggatg
     2521 tcggccgctc gatcgggagc ggcgcaggat tatacccagt ctggcagatc gtcaccagtg
     2581 cggcttcaac cgatcgaagt gggaaaaatc cctgtgagga cgcagacttg gtggatttca
     2641 tgcaagtgat tcggtaagat ggcgttacgt tgcgtgcgat ttctctacgg agtttgagtg
     2701 tcccgaccga gcgttctcgt tatcctcttt gcagtccttc tgatcgccgg tgtgtctttt
     2761 cttctgttct cgcccttcct gacgggcgac gattatctga aacaattctt tctcgagcag
     2821 ctggaacaga atcttggacg caagatcgac gtccaccgga tcaaatttgt tttgtttccg
     2881 aacattcgtc tcgaattgac ccaggtcggc atccacgacc gacagtccga ccaggtggtg
     2941 ttggcggcca aaaaagtgga tcttgtcctg cgcctcttgc cattgcttcg taagcaagtg
     3001 gtaggaaaac gactggtcat cgaagagccg accctgacgc tccggcggga tcgaaacggg
     3061 cattggaacg tgctcgatca ggttgtccaa ggagcggcta cggaccagga cgccttgcag
     3121 atgatgagcc ggatgtttcg aattcgagaa gcgacgctgg tgaatggaac ggtgaccatc
     3181 gtcgacgagg cacgtccgga cggcccgcgc tcgatgaagc tcgagtcggt ggaagctgcc
     3241 ctcaagatct gggccgaacg aggacaggcc gatgtgcatg tctccgccgt ccgtgcggga
     3301 gaggccggag tttcggcagt atctcttgcg ggaaggatcg cgcgagtcga acaaccatcg
     3361 ctcacgacga atgccccgtc acagcttgtg ctgcagtttg acggcagcat ggaagcagcc
     3421 aatctcaatc ttcgagacgt tgcggacttt ttcgggcctc gcccgattcc tgagcaactg
     3481 aaagggacga tcaatctgag cagcaccgtg agagcccagc ccggcgtcgc cggctacgac
     3541 gtactcttgt ccgatctgtc cgcaaaggcg gggcaattcg cgttgtccgg gaaagccagc
     3601 ctctccggat tgatgtcggc tcaaccgact gttgcgctga cgtttgcgtc ggctcccatc
     3661 cgattgaacg aactcctgac ggtcattccc gcgcaatgga tccatgcgca attagcggct
     3721 gtgattcagg accgtcaaat caacggacaa atcgaagccg tatctgcgac ggttacagcc
     3781 tcttccgtat cgggcccgca gttgtctgtt accggtgagt ttcacgtgcg cgacgcagaa
     3841 gcgttggtcg gagaagatcg tgttccggcc aaggatgtct ccgccgtcgt gtccgttgaa
     3901 gcagggcgcg ttcgcgtctc caatctgact ggtgtatacg gtgcgattct gatgagcgat
     3961 gggaaagcgc tggtgtcgtt cttggaagcc ggaccttggc tcgaattgga aattaccgga
     4021 acgatgccgg ctgctgacct gcttcaattt ctctccaaga ccgtgaaatc tgagcgactg
     4081 tcgcgtgtcc tggcgtcctc gcgtgaagta gagggacagg cggcgccgac gtttcgtatg
     4141 gttggtccgc tcaatcaacc gggaggcgtc acgtttgccg gtggagaaat tacggcgcaa
     4201 cagatcagcc taacgaaccc ttcgttgccg gagcgcctca cagcgcttca gggacgattt
     4261 gtgctgctcg acggggagac gcgatttgat caagttaaag ggcacctagg cgatcttgtg
     4321 gtggaagtcc agggcggaat gaccggaggg agcgccagct cgtttcagga ttttctcgtc
     4381 cgtgttcgag gcgatgccat tcatatgaca cgattgctcc cgccgaagtc acttccggag
     4441 ggggcgatcg atgggatcat caatggcgat gtcgtgctga ctggcttgac ggatgcgccg
     4501 cggttacggg gagaagtgat gttgaccgag gcgaaggtct ttttgccggg gatgattgag
     4561 aagcctgtcg gcgcaccggc ttcggtccaa tttgacgggg aactgtcaac gacttccgtg
     4621 ctcacgctga cgcgggtgga actgcagctg ccatcgcttc gccttccgat taaagggagg
     4681 ctccaattgg gcgagcgctt ctcgatcgac gccgcgctgg ccaccgggac gttatccctt
     4741 tccagcctac ccgagtggat cgccaaaggg ggatttgaag cagggaacat cgaattgtcg
     4801 ctcgatatga aaggcaaaga ccgggattgg cgaacctggc ggacgacggg atggctcgct
     4861 ctgacgaatg ggctcatgac ggccagtggc gtggacggcc cgattcaaga cctctatctt
     4921 cgtctccacc tcgtgcgcag cgcggcggag ctgaagcggt tgtcgttccg cgtggcggat
     4981 agcgatgtgt cccttgaagg gaccatccgg aactggacgg taaaaccaat cgtggccgcc
     5041 aggatcgaat cgaataacat ggacatcgat ttgctgattc ccaagggagc gcggtctcct
     5101 attcgagatt ttctcgagac gttggccgcc accagcaaag tgaatgcgac ggccaccatc
     5161 gcgcgcggcc actataaaca tctcaagttt ggcagtctct ctgccagaat cacaattcaa
     5221 gacggagtcc tcgatatcga tcggttgtcc ggtcaatcac ctgacggaga actcgccggt
     5281 cgcttcgtgg tgcagttgcc ccgtaaccaa ccggccgacg gagaggtctc cctgcgcgcg
     5341 acgggattgg tcgtggaaga tctgctgaag cttgttgggt ccaaagggcc ggtgacaggg
     5401 gaggcacgag tcagcggcac gattcgcggc cacgggcgaa atccacacgg catctaccct
     5461 acgttaaacg gcaaggtcga cgttctggtt caagacggcc gtatcttcaa gtcgcaagat
     5521 cgggcgatct ggaagattat cagcattctg aacctacccg ccgtgctcca gggcaaagtc
     5581 gatcttgaga aggaagggct gccctatagc aagatcacgg cgacggtgac ggtccgggat
     5641 ggccttttcg aaacggagaa tcttattatc gatagtccca tcatcaaggt gaccgccgcc
     5701 ggcaactatg atttgccaac cgatcaactc gacatggtct gggccgtgag tccgtttggg
     5761 tcctactcgc agttcctcaa aacgattcca ctcttcggtc gactctttgc cggtgaccga
     5821 aagggatttg ccacggccat gttctcggtc aaaggcgcga tcgaagatcc cgaaacaacc
     5881 tacctgccga tgaaatcatt tgccgtcggc ctgacggggc ttgggcaact ggccttcgac
     5941 ctcttgaaaa acaccgtcac gctgcctctt gatcttatga caccgaatga ggacaaaacg
     6001 acggacaaag aaggtcagct cacgctggaa ccggctccat ccgctccttg accgacctca
     6061 gacctcatgt tagattccga ttgagcaacg tatgactgaa tctccaccgc aagcgcctcg
     6121 tgccac
//
LOCUS       DATDNW010000112         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_764488, whole genome shotgun sequence.
ACCESSION   DATDNW010000112 DATDNW010000000
VERSION     DATDNW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764488"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..114
                     /locus_tag="VI359_02830"
     tRNA            40..114
                     /locus_tag="VI359_02830"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Asn,seq:gtt)
     gene            complement(222..3398)
                     /locus_tag="VI359_02835"
     CDS             complement(222..3398)
                     /locus_tag="VI359_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6973219.1"
                     /translation="MISRVIDWCANNRFLVFIGTLMLLLAGIWSLKNIPLDALPDISD
                     VQVIVHIPWEGQPPNIIEDQVTYPIVSTLLAAPHVKAVRAQTMFGDSYIFVVFEDGTD
                     LYWARSRVLEYIQQLQGVLPKNVSPMIGPDATGAGWVYEYAVIDRSHRHSLAELRSLQ
                     DWYLRFQLETVPGVAEVASIGGFVRQYQVNLDPNKLRAYNIPLSMVIDRVRDSTNEVG
                     GRVLEMAGARYMIRGLGYLRSLQDLESVPVATKNGTPILVRDLGTVAFGPDIREGVTE
                     WNGEGEAVGGIIVMRDGMNALTVIDGVKKRLAEIKPSLPAGVEIVSGYDRSGLIHDSI
                     KTLQRDLLLEVAIVSFVTIAFLFHFRSALVPILALPIALVASFIPMYFLRVSSNIMSL
                     GGLALAIGVLIDASIVMVENGYRHLSERMAAAAQSGTEVTDKDRKALLIGGAKQVGPA
                     IFFSLLVILVSFLPVFLLEAQEGRMFRPLAWTKTLSVAFSSILAITLVPVLMVIFIRG
                     HLRPESENPFSRWTQALYLPVLRFCLRYRKTTLLLNLIFLVVTFPLAWRIGSQFMPPL
                     YEGSSLYMPTALPGISITQASTLLQQQDRIIKSFPEVETVFGAVGRSNSPTDNAPLDM
                     YDTTIMLKPREQWRPGMTYERLIHEMDERLQFPGLTNTWTMPVQNRLDMELTGIKTPV
                     GLKIQGPNVEVIQQAGTQLQQILGGIPELRSVFAERVAEGFYINIEVNRLEAARYGLT
                     VGDVHRAVTSGIGGENITENVEGRQRYPVNVRYNRDFRDDIEELKRVVIATPTGAQIP
                     ISEVAKVYFSRGPAMIRDEDGLLTGYVYLDLATKDYGGFVMKADRLLREKLKLPPGYT
                     YKWSGEYEFELRAKSRLKIIVPIVFFVIFLLLYMVFHSATEAAVLIFPTFYAMTGGLL
                     LQYWLGYNFSVAVWVGYIALFGIAVETGVVMVVYLHESLDKRLGSGIPLKHEDIEAAA
                     IEGAVQRLRPKLMTVTAVLASLLPILWESGIGSDVMKPIAAPIVGGMVTSTIHVLILV
                     PVFFAFMKERALRAGNLRIQDRSRIVEQ"
     gene            complement(3401..>3650)
                     /locus_tag="VI359_02840"
     CDS             complement(3401..>3650)
                     /locus_tag="VI359_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY6973220.1"
                     /translation="TATDGSPVTGAQISVRSSMAAMPAMGMAAMNVVTPLAEKGSGVY
                     EGQVTLASGGTWKITITAVKDGAVLATKQLSLQAQGGM"
BASE COUNT          872 a          932 c         1065 g          781 t
ORIGIN      
        1 gacggctgta ttagaatgaa aacaaatgtg cagcgcggtt ccccggtagc tcaatggtag
       61 agcattcggc tgttaaccga agggttgcta gttcgaatct agcccgggga gccaaatctt
      121 ttgaattcaa tcgattgtga atgcgcattc tcacgttttc cttccagaaa atcttcgcgt
      181 catgtgagtg tgtttcttct tcttgttcgt tcgtcctgta ttcattgctc tacgattctg
      241 gacctgtctt gaattctcag gttgcccgct cgaagggcgc gttctttcat gaaggcaaag
      301 aaaactggca ctaggatgag aacgtggatg gtggacgtaa ccattcctcc cacgataggg
      361 gcggcaatag gcttcatgac gtccgaaccg attccgctct cccagaggat gggcaagagg
      421 ctggcgagca cagccgtgac ggtcatcaat tttggccgca atctttgtac ggccccttcg
      481 atagcggcgg cctcaatgtc ttcgtgttta agggggatac ccgatccgag gcgcttgtcg
      541 agagattcgt ggagataaac gaccatgaca acgcccgttt ccacagcaat tccaaaaagt
      601 gcgatgtagc ccacccagac ggccacgctg aagttgtaac ccagccaata ctgaaggagg
      661 agccctcccg tcatggcata aaaggtgggg aagatgagaa cagcggcttc cgttgcggaa
      721 tgaaagacca tgtagaggag caagaagatc acaaagaaca cgatgggaac gatgatcttc
      781 aggcgcgatt ttgcccgcag ctcgaactca tattcgccgg accatttgta ggtatagcct
      841 ggcggcaact tgagtttctc gcgcaagagg cggtctgctt tcatgacgaa tccgccatag
      901 tccttggtag cgaggtccag gtagacataa ccggtcagaa gcccatcctc atcgcgaatc
      961 atggcagggc cacgactgaa atagaccttg gccacttcgg agatcgggat ttgcgccccg
     1021 gtgggggtcg caatcacaac gcgcttgagt tcttcaatgt catcgcgaaa atcccggttg
     1081 taacgaacat ttacgggata gcgctgccgg ccctctacat tttcagtgat attttctccg
     1141 ccgatgccag aggttaccgc tcgatggaca tccccgacgg tcaagccgta acgggcagct
     1201 tcgagacgat tgacttcgat attgatgtag aagccctcag cgacgcgctc ggcgaatacg
     1261 gaccggagtt ccgggatgcc tcccaggatt tgttgtaatt gtgtccccgc ctgttggatc
     1321 acttccacgt tggggccttg aatcttgaga cctacggggg tcttaatgcc cgtcaactcc
     1381 atgtcgagcc gattctgcac aggcatggtc catgtgttgg tcaggccggg gaactggagt
     1441 ctttcgtcca tctcgtgaat gagcctttcg taggtcattc cgggccgcca ctgctcgcgt
     1501 ggtttgagca tgatggtggt gtcgtacatg tccagaggag cattgtcagt gggactgttg
     1561 gagcggccca cggcgccaaa gacggtctcc acttccggga atgatttgat gattctgtct
     1621 tgttgctgaa gaagcgttga agcctgcgtg atggagattc cgggaagggc ggtcggcatg
     1681 tacagagaag aaccctcata aagtggaggc atgaactggc tgccgatgcg ccaagccaga
     1741 ggaaaggtga ccaccagaaa gatgaggttt agcagaagcg tcgttttgcg gtaacgcaaa
     1801 cagaagcgaa gaacaggcag gtacagagct tgtgtccagc gcgagaaggg gttttctgat
     1861 tcgggccgaa gatggccacg gataaaaatg accatcagaa cgggaacgag cgtgatggca
     1921 agaatggagg aaaaagccac ggaaagtgtt tttgtccagg cgagagggcg gaacatcctg
     1981 ccttcctggg cctcgagaag aaaaactggc aagaacgaga caaggatgac caggagcgaa
     2041 aagaagatcg ccggacccac ctgcttcgca ccgccaatga ggagagcttt cctgtctttg
     2101 tccgtcactt cggtgcccga ttgcgccgcg gctgccatgc gttcggacaa gtggcggtag
     2161 ccgttttcca ccatgacgat ggaggcatcg atcaggacac cgatggcgag cgccaggccg
     2221 ccgagagaca tgatgttcga gctgacgcgg aggaaataca tggggatgaa ggaagcgaca
     2281 agcgcgattg gcagagccag gatgggtaca agtgcggagc gaaagtgaaa gaggaacgcg
     2341 atggttacaa agctgacgat cgcgacttcc aggagaagat cgcgttgcaa cgtcttgatg
     2401 gagtcgtgga tcagcccgga acggtcataa ccagacacga tctcgacgcc cgcaggaagt
     2461 gacggcttaa tttcggcaag tcttttcttc acgccatcaa tcacggtgag agcattcatg
     2521 ccgtcccgca tgacgatgat tccgccgaca gcttcgcctt ctccgttcca ctctgtaact
     2581 ccttcccgaa tgtccgggcc aaacgccacg gtaccgaggt cacggacgag gattggggtc
     2641 ccattctttg ttgcgaccgg aacgctttcg aggtcctgga gagaacgcag gtagcccaaa
     2701 ccgcgaatca tgtagcgagc gccggccatt tcgagaacgc gaccaccaac ctcattggtc
     2761 gaatcgcgaa cgcggtcaat caccatggaa agcgggatgt tgtaagcgcg aagtttgttg
     2821 gggtccagat tcacctgata ctgccggacg aatccgccta tggaggcaac ttccgcaaca
     2881 ccgggaacgg tttcaagttg aaagcggaga taccagtcct gaagactgcg tagttcagcc
     2941 aaactgtggc gatgagaacg atcaatgacg gcatattcat aaacccaacc cgctccggta
     3001 gcgtccggtc cgatcatagg ggagacgttt ttgggcaaaa ctccctgaag ctgctggatg
     3061 tattcaagta cgcgggagcg cgcccaatag aggtctgtcc cgtcctcgaa aacaacaaag
     3121 atgtaggaat caccgaacat ggtttgggct ctcacggcct taacgtgggg agccgcgaga
     3181 agcgtagaga cgatgggata ggtgacttgg tcttcgatga tgttcggcgg ctgaccttcc
     3241 cagggaatgt ggacaatcac ttgtacgtcg gagatgtccg gcagcgcatc gagaggaatg
     3301 ttcttgagcg accagattcc ggcaagaagc agcatgagag tcccgatgaa aaccaggaag
     3361 cggttgttcg cgcaccaatc aataacgcgg gagatcatgg ctacatgcct ccttgcgcct
     3421 gaaggctgag ctgcttcgtg gcaaggacag cgccgtcctt aactgctgtg atggttatct
     3481 tccatgttcc accactagcg agagtcacct gtccttcata gacaccgctc cctttttcag
     3541 cgagcggagt aacgacattc attgcggcca tccccattgc gggcatcgcg gccatggaac
     3601 tgcgtacact gatctgcgcg ccggtcacag gtgaaccatc ggttgcggtg
//
LOCUS       DATDNW010000113         6099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_283830, whole genome shotgun sequence.
ACCESSION   DATDNW010000113 DATDNW010000000
VERSION     DATDNW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283830"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..3220)
                     /locus_tag="VI359_02845"
     CDS             complement(77..3220)
                     /locus_tag="VI359_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6973221.1"
                     /translation="MVGRIIEFSARNRFIVFLFVFAFSSAGLWAMRQTPVDALPDLSD
                     TQVIVYTTWAGRSPNLVEDQITYPIVTALLSAPNVTVVRGLSDFGYSYVYVLFKDGTD
                     IYWARSRVLEYLNQLAGRLPEGVTAQLGPDATGVGWVFQYALVDETGQHDLAALRTFQ
                     DWYLQYWLRAVEGVAEVASIGGFVRQYQVNLDPTKVLAYKLSLPEIIETIRLSNQDVG
                     GRVVEFTGIEYVVRGRGYIQKTGDIEKIAVGVNETGTPILLRDVATVGLGPDMRRGLV
                     DLNGQGEVVGGIVVMRFGKNALEVIDRVKAKLEELKPSMPKGVKVVTTYDRSDLIRES
                     IATVKENLIEELVIVSAMLVGFLLHLRSALMPILTLPIAVLISFLPIWYFGVGMNIMS
                     IGGIIVAIGDMVDASIVMVDNAHRRLEEWEREGRIGDRTQVLIDSAKEVGPSIFASLL
                     VIAIAFMPVFTLEAQEGRLFRPLAVTKNLAIAMSAFLAITLIPALLDFLVRGRIISEQ
                     RHPISRTLQRLYAPILQLALRYRWAVIVGAFALTLLTIPPFLRLGSEFMPPLYEGTIL
                     YMPTTLPGLSVTEAGRLLQIMDRKLRSFPEVDHVFGKAGRAETSTDPAPFSMMEVVVE
                     LKSKDQWRPGLSYEGLVDEMDRALQFPGVTNAWTMPIKNRIDMLTTGIRTPVGIKIFG
                     PDLKQIETLGEHLEMLLKDVPGTRSVYAERVSSGYFLDFDINREEIARYGLKLMDVGR
                     VIESAIGGENITTTVEGRERYPVNVRYLRELRDEPDKLQRILVATPTGVQVPLAQLAT
                     LRFVNGPPMIRDENGMLAGYVYVDMAGRDMGSYVEDLKHLVREKVQLPPGYTIAWSGQ
                     YENMQRVRERLTVFIPLTITIIFVLYYFTFRSVTESLMVLLGVPLSMVGGIWSLALLG
                     YNMSIAVWVGLIALAGVGAETSAVMLAYLDEACARRQRAGTLRTLTDLIETVQEGAVV
                     RIRAVSMTSLANILGLLPVMWATGTGADVMKRLAAPMVGGVFTAMLLTLVVIPALYVI
                     WRWQMERNRNKVRAPQD"
     gene            complement(3228..4490)
                     /locus_tag="VI359_02850"
     CDS             complement(3228..4490)
                     /locus_tag="VI359_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6973222.1"
                     /translation="MTRQRFIGIAAVFIIAAAAFVWLVSRPVVLPPADSKEDLMMDMP
                     GMEKSGVGKKDAQAPPLSEKPDESDLPAESGGPSGTISIPPERLQTIGVKYQPVTRRP
                     LEKLIRTVGRVVVDERRLAKVTIKFHGWIEQLFVSATGDHVKKGQKLFTIYSPDLVAT
                     QEEYLLALQSRKQLGESEIPEVAAGSKDLLEATRHRLHLWDIGEEHIRDLERTKQVTK
                     TLPLHSPISGTVISKNVLQGTHVEPGEELYTIADLSHIWILADIYEYELSFVKIGQQA
                     AVTLSYDPGTVLSGQVGFVYPTLDPKTRTAKVRFELDNAEEKLKPDMYANVELRVSLG
                     TKLAIPKEAVIESGKQQVVFLHHGGGKLEPRLIKTGVKTEEFLEVLEGLKEGEHIVTS
                     ANFLIDSESRLKSVVESMSGMSGMKPKD"
     gene            complement(4487..5830)
                     /locus_tag="VI359_02855"
     CDS             complement(4487..5830)
                     /locus_tag="VI359_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6973223.1"
                     /translation="MKPSVAPHLGWFVLIVVFSAVVLSPWSVRGAEDRGVVAPDGTAH
                     PSHSELRIDLPGLIGDLESANPEIKAARQRWEAAKAVVPQVQTLPDPRLQLGYQRMPM
                     VPPVVEGAMYGFGQEIPFPGKLGLKGEVAQREAERLEQEFNATRLRLIATLKEAYFNL
                     HYVHKSIEIVERNKVLLMQFEKTAKARYSVGQAAQQDVFRAQVEISRVLDRLAVLDQQ
                     KESLHATINRLLNRPPAGPLGTPEEVQTTILTIPLQELSRRADEFSPALLATAKSIDR
                     SERSVSLAKRQYYPDFDITALGIRNDKINDNGYQVMVGIKIPLFYETKQKQGVREALA
                     SLEGAREDFATTRQDLLFQVKDGFVQAQRAERLITILRDAIIPQATLALQAAQASYTV
                     GKVDFLTLLNSLLILQDSQLELHGEMVNHEKALARLEAVTGGPLGSPLPERKPGS"
BASE COUNT         1304 a         1824 c         1671 g         1300 t
ORIGIN      
        1 tgagcaagcc gaggccatgg ccaggttcca tcatgaacag gacaaggcaa ttccctgagc
       61 tcgaccagcc cggtcctcaa tcttgagggg ctctcacttt gtttctgttt ctttccatct
      121 gccaacgcca gatcacataa agggccggga tcaccaccag ggtcaggagc atggctgtga
      181 agacgccgcc gaccatcggc gcagccagtc gcttcatcac atcggctccg gtgccggtgg
      241 cccacatcac cggaagtaga ccgagtatgt tggcgagcga cgtcatactg actgcccgta
      301 tccgcacaac tgccccttcc tggaccgttt cgatcagatc agtgagcgtg cggagtgtgc
      361 ccgctctctg ccgtcgggca caggcctcat ccaagtaagc gagcatcacg gcactggtct
      421 ctgccccaac gcccgccaac gcaattaacc ccacccatac cgcgatgctc atgttgtagc
      481 ccagcaaagc cagcgaccag atcccgccca ccatcgacag gggaacgccg agcagaacca
      541 tgagcgactc cgtgaccgac cggaaggtga agtagtagag aacgaaaatg atggtgatgg
      601 tgagcgggat aaacacggta agccgctctc gcacccgctg catgttttcg tattgtcccg
      661 accacgcgat ggtgtagccc ggcgggagct gtaccttctc acgaaccagg tgcttgagat
      721 cttccacata gctccccata tcccgcccgg ccatatctac atacacatac ccggccaaca
      781 tcccattctc gtcccggatc atgggcgggc cattgacgaa gcgaagagtt gcgagttgcg
      841 caaggggaac ttgcacaccc gtcggcgtgg caaccaggat ccgctgcagc ttgtcgggct
      901 cgtcacggag ttcgcgtaga taccgcacgt taacgggata ccgctcacgt ccttcgaccg
      961 tggtcgtgat gttttcaccg ccgatggcgg actcgatcac tcgacccacg tccataagct
     1021 tgagcccata ccgggcgatc tcctcgcgat tgatgtcgaa gtccaggaag tacccgctcg
     1081 acacccgttc ggcatagaca cttctcgtac ccggtacgtc cttcaatagc atttccaaat
     1141 gttcgccgag tgtctcgatc tgcttcaaat cgggtccgaa gatcttgatc ccgaccggcg
     1201 tccgaatgcc ggttgtcagc atatcaatcc gatttttgat cggcatggtc caggcattag
     1261 ttaccccggg gaattgaagc gctcggtcca tttcatcgac cagtccttcg tagctcaatc
     1321 ccggtcgcca ctggtccttc gatttcagct cgaccactac ttccatcatg ctgaagggcg
     1381 ccgggtcggt ggaggtttca gcccgcccgg ctttgccaaa gacgtggtcg acttcaggaa
     1441 aggagcgcag tttccggtcc atgatctgca acagccgtcc ggcttccgtg acagaaagcc
     1501 ctggcaaggt cgtcggcata tagaggattg ttccttcata gaggggcggc atgaactcgg
     1561 agccgaggcg taaaaacggc gggatggtga gcaacgtgag agcgaaggcc ccgacgatca
     1621 cagcccagcg atatcgtaag gctaactgaa ggatcggcgc gtacagccgt tgcagagtcc
     1681 ggctgatcgg atgacgctgc tccgagataa tgcggccacg aacgaggaaa tccaacaatg
     1741 ccggaatgag tgtgatcgcc aggaacgcac tcatcgcgat tgcgaggttc ttggtcaccg
     1801 cgagtggtcg gaacagccgt ccttcctgag cctccagtgt gaagaccggc atgaacgcaa
     1861 tagcgatcac taacagcgag gcgaagatcg aagggccaac ttccttggcc gagtcgatga
     1921 gaacctgcgt cctgtcgccg atgcgtccct cacgttccca ctcttcaagc cgccggtgcg
     1981 cattgtcgac catgacgatc gaggcatcca ccatatcgcc gatcgctacg atgatcccgc
     2041 cgatcgacat gatgttcatc cccaccccga agtaccagat cgggagaaag gaaatcagca
     2101 cggcgatcgg gagcgtcagg atcggcatca aggccgagcg caggtgcaac aagaatccga
     2161 caagcatcgc gctcacaatg accaattcct caatcagatt ttccttcacc gtcgcaatcg
     2221 actcacggat aaggtcactt cggtcgtagg tggtgaccac cttgacgcct ttgggcatgg
     2281 agggtttcaa ttcctcgagc ttggccttga cccggtcgat cacctccagc gcattcttgc
     2341 cgaaccgcat gacgacgatc ccgcctacta cctccccctg cccattcaaa tccaccaaac
     2401 cccggcgcat gtccggtccc aggcccacgg tcgccacgtc gcgcaacaga atcggcgtcc
     2461 cagtctcatt cactccgacc gcgatcttct caatgtcccc ggtcttctga atataccctc
     2521 ggcccctgac gacgtactcg attcccgtga actcaacgac gcggcccccg acatcctggt
     2581 tgctgagccg gatagtttcg ataatctccg gaagcgaaag tttgtaggcc aatactttgg
     2641 tgggatcgag attgacctga tactgccgca caaacccgcc gatactcgcc acttcagcga
     2701 cgccttctac ggcccgcaac caatactgga gataccaatc ctggaaggtt cgcaaggcag
     2761 ccagatcgtg ctggccggtc tcgtctacga gagcatattg gaagacccac ccgacgccag
     2821 tggcgtcagg gccgagttgc gcagtcaccc cttccggcag acggccagcc agttggttca
     2881 ggtactcgag gacacgggag cgtgcccagt agatgtcggt gccgtctttg aagaggacgt
     2941 agacatagga atacccaaag tctgagaggc cgcggacgac ggtcacattg ggagcagaca
     3001 gcaacgcggt cacaatcgga taggtgatct ggtcctctac caagttggga gaccgcccag
     3061 cccaagttgt gtagacgatc acctgggtgt cagaaaggtc tggcaaggca tcgaccggcg
     3121 tctgccgcat ggcccagagg cctgccgatg agaatgcaaa gacaaacaga aagacgatga
     3181 agcggtttct cgcgctgaat tcgataatgc gtccaaccat ggcctgatca atccttcggc
     3241 ttcatcccgg acatgccgct catgctctcg acgaccgact tgagtcgact ttcactgtca
     3301 atcagaaaat tcgccgacgt gacgatatgc tcaccttcct tcagcccctc cagcacttca
     3361 agaaactctt cggtcttcac ccccgtttta attaggcgcg gctccagctt ccccccgccg
     3421 tgatgcagaa acaccacttg ctgcttaccg gactcgatga ccgcttcctt cgggatcgcg
     3481 agcttggtgc cgagactcac tcgcagctcg acattggcat acatgtctgg cttcagtttc
     3541 tcctcagcgt tgtcgagttc gaaacgcacc ttggccgtcc gagtcttggg atcaagtgtg
     3601 ggatagacga agcccacctg tccgcttagg accgttcccg gatcgtagga caatgtcacg
     3661 gcggcctgtt gcccgatctt cacgaaggag agctcgtatt cgtagatgtc tgccaagatc
     3721 catatgtgtg agagatccgc gatggtatag agttcttctc cgggctcgac atgtgtgccc
     3781 tggagaacat tcttgctaat gacggtcccg ctgatcggcg aatggagcgg cagcgtcttc
     3841 gttacctgct tcgtccgttc taaatcccgg atatgttcct cgccgatgtc ccagaggtgg
     3901 aggcgatgac gggttgcttc gagcaaatcc ttggagcccg cagccacttc gggaatctcg
     3961 ctttccccca gctgcttccg actctgcaat gccagaagat attcttcctg tgtcgcgacg
     4021 agatcaggac tgtaaatggt gaagagcttt tgtcccttct tcacgtgatc gccggtcgca
     4081 ctcacaaata gttgttcaat ccagccatgg aatttgatcg tgaccttcgc gagtcgccgt
     4141 tcatcgacca ctacacggcc cactgtccga atcagcttct ccagcggccg cctggtcaca
     4201 ggttgatatt tcaccccgat ggtttggagc cgctcaggcg ggatggagat cgttccggac
     4261 ggccctccgg actcagccgg taggtcggat tcatccggct tttctgagag cgggggagcc
     4321 tgggcgtcct tcttccccac gcctgacttc tccatcccag gcatatccat catcaggtcc
     4381 tctttggaat ccgccggtgg cagaacaacc ggtctcgaca ccagccagac gaatgcggca
     4441 gcggcgatga tgaagaccgc tgctataccg atgaaccgct gacgagtcat gatcctggtt
     4501 tcctttccgg cagcgggcta cctaatggtc cacccgtcac ggcttcgagc cgggcgagcg
     4561 ctttctcgtg gttcaccatc tccccatgca gttcgagctg gctgtcctgc aagatcaaga
     4621 ggctgttgag cagcgtcagg aaatccactt tgcccaccgt gtaacttgct tgcgcggcct
     4681 gcagggccag cgtggcctgg gggatgatgg catcacgcag aatcgtgatg agccgctcgg
     4741 cgcgctgcgc ttggacgaag ccatccttca cttggaacaa caggtcctgc cgagttgtgg
     4801 cgaagtcttc tctcgctccc tcaagactgg cgagggcctc tcgtacgcct tgcttctgtt
     4861 tagtctcata aaaaagtggg atcttaatcc cgaccataac ctgatagccg ttatcattga
     4921 ttttgtcgtt tcggataccg agggccgtaa tatcgaaatc agggtaatac tgacgcttgg
     4981 cgagcgagac ggaccgctca gagcgatcga tgctctttgc tgtcgccaac agtgcggggg
     5041 agaattcgtc ggctcggcgg ctgagttcct gtaatgggat cgtcaaaatg gtggtttgaa
     5101 cttcttctgg tgttcctaga ggtccagctg gcggacggtt caagagacgg ttgattgtgg
     5161 cgtgaagact ttccttttgt tggtctaaca ccgccaaccg atcgagaaca cgtgaaatct
     5221 cgacttgggc tctgaacaca tcctgctggg cggcttgtcc gacgctgtac cgcgctttgg
     5281 cggttttctc gaactgcatc aagagcactt tgtttctctc cacgatctcg atgctcttgt
     5341 gcacatagtg gagattgaaa taggcttctt tgagcgtcgc gatgagcctc agccgcgtgg
     5401 cattgaattc ctgctccagt cgctctgctt cacgctgggc tacttcccct ttgagaccga
     5461 gcttgccggg aaatgggatt tcctgtccga acccgtacat cgcgccttcc acaacaggag
     5521 gaaccatggg catgcgttga tagccaagct ggagtcgagg gtccggcaac gtttggactt
     5581 gcggcacgac agccttcgcg gcttcccaac gctggcgggc agccttaatt tcggggttgg
     5641 cgctttccag atctccgatg agacccggaa ggtcaatgcg aagctcacta tgcgagggat
     5701 gagcggtccc gtctggagcg actaccccac gatcttccgc tccacgaacg gaccagggtg
     5761 aaagcaccac ggccgagaag acgacgatca gaacaaacca ccctagatgg ggagcaacag
     5821 atggtttcac ggtgacacct ccaggcgact ttgggaaaat ggatcaactg ccgctcagcc
     5881 tgagcagcca agcagcgagt gaacgcactg gaagtgtgga atcagattcg gaggaccgag
     5941 gaagaatcta tggagatagg gacaatggaa cacgcgcggc tccaggacca ctgcgtcgag
     6001 acggatggaa ctgtgagcac tacgccaaga ctgagcacga tcggtccgtc atcaagatct
     6061 atggccgtct cattcttgac ctggcgttcc ggcgatgag
//
LOCUS       DATDNW010000114         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_900286, whole genome shotgun sequence.
ACCESSION   DATDNW010000114 DATDNW010000000
VERSION     DATDNW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900286"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VI359_02860"
     CDS             complement(<1..915)
                     /locus_tag="VI359_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973224.1"
                     /translation="MVSDPCRNRQPFRSIPSLLEALSAGLLMVLSLLPAEVLAADTTP
                     SAEAQSQDNTAAESTEEKPGGLRGRIESRFYGATPEERERLEEERKRLSAAAAVFGTD
                     PTAINGYYQLEYGHNTFLHNLRTDTATAEARLPITPNFVMRVTMPYVWADLNQPRGFT
                     TNGASDLLVRFGGRVYASPELAVFVGGDATFPTGSNDRLSTGKYTLGPGFATAVPLPR
                     LRTLFFTFVQDFNSVGGDPSRANLHFMRVTSAFNTIWSERWWTTASLEWDMNWNNNRK
                     TTMNLLGEIGHRLDKHWSVFASYGAGVMG"
     gene            complement(916..2016)
                     /gene="ydiK"
                     /locus_tag="VI359_02865"
     CDS             complement(916..2016)
                     /gene="ydiK"
                     /locus_tag="VI359_02865"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEY6973225.1"
                     /translation="MPRPASNQDITRTVFAVLFIGGLIAGSFWILWPFLPAVVWATMI
                     VVASWPLLMRVQQWLWGRRWLAVAVMTCILLLVLVVPLSLAIGTIVSNAHVITGWAGA
                     LTTLQLPQPPDWLRALPLVGKRAAQTWGDLAALGQEDLAKKLTPYAGAVISWFVAQIG
                     GFGMMTGQFLLTVVVSAILFASGETAAEGVRHFGRRLAGVQGESTVVLAGKAIRGVAL
                     GVIVTAFVQAILSGIGLAITGVPFAAILTAIIFMLAVAQVGAGLVMLGAIIWLFWAGS
                     IGWGVVLIVWTILTGPVLHNTLRPILIKKGADLPLLLVLAGVLGGLIAFGLVGIFIGP
                     MVLAVSYRLLQAWVTEELAEPTPRAERTHEAG"
BASE COUNT          628 a          848 c          802 g          550 t
ORIGIN      
        1 tcccatcact cccgcgccgt atgaggcaaa gacgctccaa tgtttatcta accggtggcc
       61 gatctcgccc agcaggttca tcgtggtctt tcggttattg ttccaattca tgtcccattc
      121 cagggacgcg gtggtccacc atcgctcaga ccagatggtg ttgaatgctg acgtgactcg
      181 catgaagtgc aggttggctc tgctgggatc acctccgaca gagttgaagt cctggacaaa
      241 tgtaaagaat aacgtgcgca gcctgggcaa cgggaccgct gtcgcgaacc ctgggccaag
      301 ggtatatttc cctgtgctca gtcgatcgtt cgagcctgtc ggaaatgtag catcgccgcc
      361 gacgaacacc gccaactccg ggctggcata cactcgcccg ccaaatcgaa ccagtagatc
      421 actcgcgcca ttcgtcgtga atccgcgagg ctggttcagg tcggcccaca catagggcat
      481 cgtcacccgc atgacaaagt tgggtgtgat gggcagtctg gcctctgcag tcgccgtatc
      541 ggtgcgcaga ttgtgaagga acgtgttgtg cccgtattcg agttgatagt atccgttgat
      601 ggcggtcggg tcggtcccga ataccgctgc tgccgccgac aggcgcttgc gttcttcttc
      661 gagacgctcg cgctcttcgg gtgtggcgcc gtagaaccgc gactctatcc ggccacgaag
      721 gccccccggt ttctcctcgg tcgattcggc agctgtattg tcttgcgact gtgcttcagc
      781 agagggggtg gtatctgccg caagaacttc cgctggcaga aggctgagca ccatgagcaa
      841 cccggcgctc agagcttcca ggagtgatgg aatggatcgg aagggctgtc gatttctgca
      901 cggatctgat accacctagc ctgcctcgtg ggtgcgctcc gcacggggcg tcggctcagc
      961 cagttcttca gtcacccaag cctggagaag ccggtacgag acggccaaca ccatcggacc
     1021 gatgaagatc ccgacgagac cgaaggcgat caggccgccg agcactcccg cgagaaccaa
     1081 aagcagcggc aggtccgccc ccttcttgat caagatcgga cgcaacgtat tgtgtagaac
     1141 ggggccggtc aggatggtcc acacgatcaa caccaccccc cagccgatgg atcctgccca
     1201 gaacagccag atgatcgctc ccagcatcac cagtcccgct ccgacctgag ccaccgcgag
     1261 catgaagatg atggcagtca ggatcgccgc gaagggcacc ccggtgatgg caaggccgat
     1321 gccgctcaga atggcctgga cgaacgccgt cacgatgacg ccaagtgcga cgccacgaat
     1381 cgctttccca gcaagaacga ccgtactttc cccctgcact ccggctaacc gtcggccaaa
     1441 gtggcgtacg ccttcagccg ctgtttcgcc gcttgcaaag aggatcgcgg agacgactac
     1501 agtgagtaag aactgaccgg tcatcatccc gaaaccgccg atctgcgcaa cgaaccagct
     1561 gatcactgcc ccagcatagg gtgtgagttt cttggccaaa tcctcctgtc ccaaagcggc
     1621 aaggtcgccc cacgtttgag ctgctcgctt gccaaccagc gggagcgccc ggagccagtc
     1681 tggaggttgc ggaagctgaa gtgtggtgag cgctccggcc catccggtga tgacgtgggc
     1741 attggagaca atggttccga tggccagcga cagcggcacg acgagcacaa ggagaagaat
     1801 gcaggtcatt accgcaacag caagccagcg ccgcccccac agccattgct ggacgcgcat
     1861 gaggagaggc cagctcgcga cgacgatcat agttgcccag acgacggcag ggaggaacgg
     1921 ccacagaatc cagaacgaac cggcgataag tccaccgatg aacagcaccg cgaataccgt
     1981 gcgtgtgatg tcttgattgc tggcaggtct aggcatgagg gagcccctcc gttattttga
     2041 ctatgccttc gtggtgcggt tgttttcgtc cctcgactgc cggggacatg tcacctatgt
     2101 caaaattcca gactcgcacg tcgagagaga ggccacctaa caacgtcggg agcttcagtg
     2161 ccggtgttcc cgctcgcgca tgcgcatatc aggacaactc gggacagggt tcgagctctg
     2221 aatgtcgaac gagggcatca atctcatttt cgaaaattac gccgtatctc aggcacagcg
     2281 ggcgagaccg actttctgat cctgtttgcc cgcccttata ccatagatcg acaagaatag
     2341 gcgtatacaa gcacagcaaa agctcgtcaa acacttggat gccccgttca gaaggaatct
     2401 caacaaagtc agcttgaccg attcttccac tggctgtagc attcggccct cgctggcaag
     2461 ggccgatttc gttgactaga aaggtttagt ctcgcaaatg cgtcgcagcc tcggcgagat
     2521 cgccgatccc gatgaacggg agtcgtcgat gagaattccc acaatgctgt acctcaacga
     2581 cgggcggata gatcggctgc ctccaggcgc cacgagcctg atccagaacg atccagactg
     2641 tcaacgactt atgcaggata ttgaaagggg taaataatga ttgttcggac cgtacgccgg
     2701 gcggtgcgca ccatccaaat catgagcggt tacggctttg agcccacgat ctcttcaacc
     2761 actgtttcag ccttgcgtcg cgatctcccc acttgagctt catgaccaga atcgcatcaa
     2821 aaacgcca
//
LOCUS       DATDNW010000115         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_968038, whole genome shotgun sequence.
ACCESSION   DATDNW010000115 DATDNW010000000
VERSION     DATDNW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968038"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VI359_02870"
     CDS             <1..219
                     /locus_tag="VI359_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6973226.1"
                     /translation="EVRTKVQTGKSDIPLDYRLLNKSGDWRVYDVVVDGVSLVNNYRG
                     QFTKILRSSGYPELIEQLRKKSNHLNSP"
     gene            237..1439
                     /locus_tag="VI359_02875"
     CDS             237..1439
                     /locus_tag="VI359_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6973227.1"
                     /translation="MYNLRGFVAVVLVVVAASAVACPNDVAAEAQYFPVPSVSTSKND
                     GNDAGLIVPILITDPDGELRYIVAPMFINNSIVGNKGIINVFRYEPGGRQMRFIGSFA
                     EKIERRVLFDYTDPAFGGGRYFLNFGGTFFKNATARFFGLGDSTNEDEQTNYTAREIR
                     GNWKFGIYANEVTQIAIGQRIRDVQLQQGATDLPFTGTVFPTVPGVQGHTTIVGHRAS
                     FHYDTRNNLVTPTDGMAVNAYAELNQNTSNSQHPVYSRYELEMKKLLPSESKRAILVV
                     RADLQATIGDQVPFFEQSSLGGQNNLRGYGVDRYIDKNLVAFSVEERLHVARTRIAGV
                     MADFEIAPFVDTGQVFNSWKDVSFTNYRVTPGLGFRGIIRPNVVGRVDYGFSKEGGAV
                     FAGLDFPY"
     gene            1441..2484
                     /locus_tag="VI359_02880"
     CDS             1441..2484
                     /locus_tag="VI359_02880"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEY6973228.1"
                     /translation="MKLSGKFRNPFCSPCLIAGVLIVVCFLPGFVWAEGFGPFPVRNF
                     QPFQQLVLSLPGDRATVVKPGTLDVRLELAETSSIFDSGSPQTTVTVKFETLRSGLFL
                     RYGATEKLELGVEVPLLYRYDGFMDGAIKQVERVTTGLSPARAALNNTNFAFNVTHSG
                     QTVMSGGPGATGLGDTMLVSKYQLLTESASTPAVSVRGAVKLPTGDQSAFFGSGSPDF
                     GLGLQMEKVVAGRFVLYANLNGVLPTGSIAGFSLQPTLSAMVIAEYLWSENFSITAHF
                     DFYSTPFHGTGSDVFDKGVTETVLGFSYRIAPHWLWQVYAVENLDFITGSAADFTLST
                     LITYRFELDSSRR"
BASE COUNT          582 a          711 c          755 g          608 t
ORIGIN      
        1 gaagtccgca cgaaagtgca gaccgggaaa tcggacatcc cgctcgacta ccggttgctc
       61 aacaagtccg gcgactggcg tgtgtacgac gtggtggtgg atggtgtgag tcttgtgaat
      121 aactatcgag gacaattcac gaagatcctc cgttcctcag gctatccgga gttaatcgag
      181 caacttcgca agaaatccaa tcacctcaac tccccatagc tctgtccgga acgtccatgt
      241 acaaccttcg cggctttgtc gccgttgtac tggtagttgt ggcggcgagt gccgttgcgt
      301 gtccgaacga tgtagcagct gaggcgcagt acttccccgt cccctccgta tccaccagca
      361 agaacgacgg caacgatgca gggttgatcg tccccatcct gatcacggac ccggacgggg
      421 aactccgcta catcgtcgcc ccgatgttca tcaacaactc gatcgttggc aataaaggga
      481 tcatcaacgt gtttcgatac gaaccgggtg ggcggcaaat gagattcatc ggatccttcg
      541 ccgagaagat cgaacgaagg gtgctctttg actatacgga tcccgcgttc ggcggtggac
      601 gttattttct gaacttcgga gggaccttct ttaagaatgc caccgcccgg ttctttggat
      661 tgggagattc caccaacgaa gacgaacaaa ccaactatac ggctcgggag atcagaggca
      721 actggaagtt tggaatctac gccaatgagg tgacgcagat cgcgatcggc caacgaattc
      781 gcgatgtgca gttgcagcag ggtgcgacgg atctcccctt caccggaact gtgtttccga
      841 ctgtccctgg agtccaagga cacaccacca tcgtgggcca tcgggccagt ttccattacg
      901 atacgcgcaa caatctggtg actcccaccg atgggatggc cgtgaacgcc tatgcggagt
      961 tgaatcagaa caccagcaac agccaacatc ccgtctattc tcgttatgag ctggagatga
     1021 aaaagctcct tcccagtgaa tcaaagcgcg ccatcctcgt cgtacgcgcc gatcttcagg
     1081 caacgatcgg cgatcaggtt ccgttcttcg aacagagctc gctaggaggg caaaacaatc
     1141 ttcggggtta cggcgttgat cgctacatcg acaaaaatct cgtcgcgttc agtgtggagg
     1201 agcggctcca tgtggcacgg acacgaatcg cgggcgtgat ggcggatttt gagatcgcac
     1261 cgttcgtgga tacgggacag gtcttcaaca gctggaaaga cgtcagtttt acaaactacc
     1321 gagtgacgcc cggcctcggg ttccgcggaa tcatccgacc caacgtcgtg ggccgagtcg
     1381 actatgggtt cagtaaagag gggggggcag tatttgccgg actggatttc ccgtactaag
     1441 gtgaaactga gcggcaagtt ccgcaatccc ttttgctcac cctgtctcat tgcaggggtg
     1501 ctgatcgtcg tgtgttttct accgggattc gtgtgggccg aggggttcgg tcccttcccg
     1561 gtccggaatt ttcaaccctt tcaacagttg gtcttgagcc ttccgggtga ccgcgccacg
     1621 gtcgtcaagc ccggaacatt ggatgtgcga ttggaattgg ccgagacatc gagcatcttt
     1681 gatagtgggt ccccccagac cacggtcaca gtgaagttcg agactctccg atctggtctc
     1741 ttccttcggt acggcgccac tgagaaattg gaactggggg tcgaggtgcc gctgctctac
     1801 cggtacgatg ggtttatgga tggggccatc aaacaggtcg agcgcgtgac gactggcttg
     1861 agccccgcgc gagcagcgct caacaacacc aacttcgcct tcaatgtgac ccatagcgga
     1921 cagacggtca tgagcggggg tcctggcgca accgggctgg gagataccat gcttgtcagt
     1981 aagtatcagc tcttgaccga aagcgcatcg acgccagctg tctcggtcag gggtgctgtg
     2041 aagctgccga caggagatca atcagccttt tttggaagcg ggagcccgga ctttgggttg
     2101 ggtctgcaga tggagaaggt cgtggcggga cgatttgtgc tatacgctaa ccttaatggg
     2161 gtcttgccca ccggcagtat cgcggggttc tctttacagc cgaccctctc tgccatggtg
     2221 atcgcggaat atctatggtc ggagaacttc tcgattactg cgcatttcga cttctactcc
     2281 acaccatttc acgggacggg ctcagatgtg tttgacaagg gtgttacgga aacggtcctc
     2341 gggttcagct atcgcattgc tccgcactgg ctctggcagg tctatgcagt ggaaaatttg
     2401 gattttatta ccgggagcgc cgcggatttt accctctcga ctctgatcac ctatcgattc
     2461 gaattggact caagtcggcg atgaacgtga ggaaatgagc taaccgcttg acagtatggg
     2521 agttgtatgt gaaacttaac accgctagct cacaagccac cttgctactt catctgagcc
     2581 tgtctgaacc tagactattg tcaccaggcc tctactttca cgcaaaggag tgcggtgtat
     2641 gtcgatcttg aagagc
//
LOCUS       DATDNW010000116        12318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_284002, whole genome shotgun sequence.
ACCESSION   DATDNW010000116 DATDNW010000000
VERSION     DATDNW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284002"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..465)
                     /locus_tag="VI359_02885"
     CDS             complement(148..465)
                     /locus_tag="VI359_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973229.1"
                     /translation="MSSLPLMLKKEGLVEKHQVEGVDPSERYFNRAILVNRTPSGYSS
                     KVMYEALTVEGHTHPTIAAAVKELVEKLQGLGFSRLRTRTNFKGTRYLAEKETWIDYQ
                     DRS"
     gene            600..1151
                     /locus_tag="VI359_02890"
     CDS             600..1151
                     /locus_tag="VI359_02890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6973230.1"
                     /translation="MSRTSMNIPNSLTILRILLIPVYIGLLVYEQYDLALFVLLLAGV
                     TDALDGTIARVTNQRTRLGTFLDPLADKLLLTSGFIALSAIHLIPSWVTIVVVSRDLM
                     LLLGTAVAQFTETPIDITPTFLGKGTTFLQLTYVLLVIFLSSRRIDLSLIDPLLAGMV
                     AFTLLSGLHYLYRGYRHSNTQSA"
     gene            1312..2535
                     /locus_tag="VI359_02895"
     CDS             1312..2535
                     /locus_tag="VI359_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY6973231.1"
                     /translation="MATFAYVGRNRSGAVKKGELTAKTRDEAVDQLRKQNVVVTSLEE
                     RAGKGGGFNLNSLMGSGLTDKDLVVFTRQFGTMINAGLPLVQCLEILSTQSENKVLRA
                     TVGEVKTHVEAGSTFSDALRRFPKVFDDLYVNMVHAGEVGGLLDTILTRLAKHIEKAM
                     KLKGQIKSAMVYPSAIIGVAVIVISVLMIWVIPIFAKMFNELSGGKVGLPGPTQLVID
                     VSNIFQSYWYVMLGVIVTLVFGIKKYYATAPGRMNIDRLLLKIPVVGDLIRKASVAKF
                     TRTLGTLISSGVPLLEGLLICAKTSGNKVIEEVLINARVSISGGKTIADPLAKSEVFP
                     KMVTHMIAVGESTGALDAMLGKIADFYEDEVDQAVASLTSLLEPVMMVVLGVVIGFIV
                     IAMYLPIFQMANAIG"
     gene            2698..4395
                     /locus_tag="VI359_02900"
     CDS             2698..4395
                     /locus_tag="VI359_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973232.1"
                     /translation="MKDLRNRLHWLMALRVAVVTLLLGFSLAFQASNGERVETFYTLI
                     IATYALTIPSALLLRSLSTPLALTTFFWAQVGIDFALETMLVVKTGGVESPFAVLYVI
                     TVAVASLVPRRRVGVLTACSCMILFGIVTNIQLYGLLEPWGWLPKSRLTGPETLQTFG
                     AYGLAFLVVGFLSGALADQLQQADQSLREKEQGLTRLQAFHENIVQSISSGVFTTDAS
                     GGITSFNPAAQEATGYTLSQVLGRPWREVFNWHPNQQSDERLAMTLPTMVRFDVECKR
                     ADGNRLILGMTLSPLHEQGHQTGLVGVFKDLTQIRDLEEEMHRKEWLASLGEMSAGMA
                     HEIRNPLGALAGAMQMLRKDVTGDETNHRLMDIAIREATRLDTIITEFLQYARPPALN
                     LVECNLHKILAETLDLVQHEARTRSHIKIITSLATGPLTAQVDQDQLKQVFWNLSMNA
                     FDAMPEGGQLTISTGCRPIDMGGKKCDVIEIAFHDTGEGIPKQLLDKIFLPFFTTKKQ
                     GSGLGLAAVHRIVDLHGGWIKVESQEHQGSRFVVCLPRSGGEGMRLWHEGRTPWKKS"
     gene            4380..5762
                     /locus_tag="VI359_02905"
     CDS             4380..5762
                     /locus_tag="VI359_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973233.1"
                     /translation="MEKILVVDDEKSLREVLSIMLKRAGYTVTEACDGDEAIGHVNKE
                     IYDLVITDLRMPKADGMDVLKAVKSSSPETVVLVITAFATSDSAVEAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLSTENMLLKREMASQSSFAKIVGQSEAMQKVFDVVKKVA
                     DARSNILICGESGTGKELVARAIHYNSARSPMPFVAVNCSAVPETLLESELFGHVKGS
                     FTGAISNKAGLFEVANGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDMKVDV
                     RVIAATNRDLEKAVAEGAFREDLYYRLDVIPIRLPPLRLRTGDIPLLVTYFLEKFSQD
                     SAKPAPMLTPEALHVLLEHEWRGNVRELENLIERVVAFSSGNPVTEADVRGWLHRSVT
                     LQQPGMPTDLPDDGLDLEALINGIEKDLLLKALERAKWVKKKAARLLRLNTRSFRYRL
                     EKYAIKGGRD"
     gene            5755..6663
                     /gene="ispE"
                     /locus_tag="VI359_02910"
     CDS             5755..6663
                     /gene="ispE"
                     /locus_tag="VI359_02910"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY6973234.1"
                     /translation="MTKASPPSSELTILAPAKVNLILRILDRRSDGYHNLWSVMQTVA
                     LEDEMHIRLRPGHEDIRLRCNVSELATDRTNLVHRAASAVLARAGQSSGIDVDLRKRI
                     PMGAGLGGGSSDAAATIIGLNQLLQLNWSMTQMTEVGQSLGSDVSFFLYGPTAIVSGR
                     GETVRPMGIEGTRWAVLVNPGFGVETKWAYNELASTRGGVRPLGRLHQEIDHQQRLTW
                     SELSAAAENDFESPVFAAHEVLREIKQTLLDQGADIALLSGSGATVFGLFANEAEARR
                     AEAHFLRDNRLKVYVVLTCSGPVVCH"
     gene            6867..7808
                     /locus_tag="VI359_02915"
     CDS             6867..7808
                     /locus_tag="VI359_02915"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY6973235.1"
                     /translation="MNRELKIFSGNANPALAQEICAYLGQKLGEATVSSFSDGEIRVK
                     IEENVRGADVFVVQSCCQPVNDSLMELLIIIDALKRSSANRITAVVPYFGYARQDRKD
                     QPRVPISAKLVADLISTAGTDRVLTMDLHAGQIQGFFNVPVDHLYALPVLLDYITKKK
                     FNDLVVVSPDAGGVERARAFAKRLQANLAIIDKRREGPNQTQIMNIIGDVEGKSVLLL
                     DDMIDTAGTIVQGAQACADKGAREVWTACTHAVLTGPALERLQKSCLKQVIVTNTIPL
                     RGKEQTCPKLHQLSVAPLLGEAIRRIHEDESVSSLFA"
     gene            7857..8651
                     /locus_tag="VI359_02920"
     CDS             7857..8651
                     /locus_tag="VI359_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY6973236.1"
                     /translation="MKFDLAVTVREKSGKGAARQLRREGKVPAVLYGQGECLLVTINP
                     DALNKILKSHAGSTALISLAITGAKAKPKRTALLRDFQVDPVEGHVLHADLFEISMDK
                     PIRVKVPLHVIGGTPAGVKEGGILHHNMRELHIECLPSALPDFIEVDASPLNIGQGIH
                     LKEITKKDGLRYLDDPDQMVVSVAAPMSDAKLEALLTSQAVGPEGAKEPEVVAKGKAA
                     AEGEEGAAAAPGAAAPAGAEAKPGEKKEAAAAAAPKAEKKEPEKKK"
     gene            8654..9247
                     /gene="pth"
                     /locus_tag="VI359_02925"
     CDS             8654..9247
                     /gene="pth"
                     /locus_tag="VI359_02925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY6973237.1"
                     /translation="MRLIVGLGNPGIAYAKTRHNVGMWVVEQAAVRWSIRLATRGTTQ
                     RGSGRLGSELLELAGTLDWMNVTGPPLKGLLREFKLTADDLIVIHDDLDLEPGRLRIK
                     QAGGDGGHNGIKSIIEALDTQQFVRLKIGIGRPAPHQDSADYVLEPFTNDELEVLTPC
                     LDRAVEALECLIHRGTPTAMNQFNVREKPAEEDGEEG"
     gene            9253..10344
                     /gene="ychF"
                     /locus_tag="VI359_02930"
     CDS             9253..10344
                     /gene="ychF"
                     /locus_tag="VI359_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY6973238.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTSGGALAANYPFATVDPNTGIA
                     LVPDPRLQILTDIFQSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLGHIREVDALLH
                     IVRCFQGTDVVHVSGSVNPIRDIGVIETELMLADLDALDRRRQKTEKKVRAGDKKAAF
                     EVDFLTRLIGLLDKGEWLGNLTYTPDERSLLNECQLLSAKPVLFVANVSEGKNADDSM
                     VKTVRDFAEKRGARVVTICGQLEAELSSLPESERADFLKEMGLTESGLVRLTREAYTL
                     LTIITFFTAGETESRAWPIPKGMKAPQAAGKIHSDMERGFIRAEVYHYDDLMACGSEA
                     KVKEKGLFRLEGKDYVIKEADIVYFRFNV"
     gene            10518..10865
                     /gene="rpsF"
                     /locus_tag="VI359_02935"
     CDS             10518..10865
                     /gene="rpsF"
                     /locus_tag="VI359_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY6973239.1"
                     /translation="MELYESLFIIRPSVSDEETNALIEKMKGVADKTGAQFIKAENWG
                     RKKLAYEVRRERKGTYVYFYFKAPNNTVGELERAYRLEDNIIKFLTVHLEKELVPRRP
                     MEAPSEEFAGGRI"
     gene            10852..11250
                     /locus_tag="VI359_02940"
     CDS             10852..11250
                     /locus_tag="VI359_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY6973240.1"
                     /translation="MAGFNKVILMGNLTRNPELRYTPSGTPVASFGLATSRRFKQGEE
                     LKEEVCFVDIVVFGKQAEHCGQYLSKGNGAIVEGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFMPKRSEASGGGEPSMPEDVSFESEEHA"
     gene            11266..11538
                     /gene="rpsR"
                     /locus_tag="VI359_02945"
     CDS             11266..11538
                     /gene="rpsR"
                     /locus_tag="VI359_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY6973241.1"
                     /translation="MDRERGGDGGGREGSGGSGRFFQRRRPCRFCLDKGSIDFKDVGL
                     LRNFLTERGRIVPRRISGNCLGHQRELTVAIKRARHIALISFAEER"
     gene            11553..12173
                     /locus_tag="VI359_02950"
     CDS             11553..12173
                     /locus_tag="VI359_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY6973242.1"
                     /translation="MDLLIGDAMDLLTPKIADIAAAGLSLSGEVTAEELGLSEADAVV
                     KAPLAVTMDLAKADGLITVTGVVEGTIVRQCVRCLREYDDPLAFSIRAAYAPDVKPVQ
                     RRPKQADMRKTQLVPVEAEEDSEEEPDDQYHYQGDHVELAPMVREHVILAAPMQPLCK
                     EDCAGLCASCGKDLNEGPCFCAVDPPVTTFRVVRNTKQQTGDHGAS"
BASE COUNT         2782 a         3206 c         3705 g         2625 t
ORIGIN      
        1 tatggatcga tccgacctgc gagttggaag tcgtactttt atcgaaccgc gtaaaccctt
       61 cgaggaagaa tgagaagatt cgagagttcc gccccctgat ccacgatctc gtatatcggg
      121 aatatgttgc cggacgatag agtgaactta ggagcggtcc tgatagtcga tccaggtttc
      181 tttttcggcg aggtatctgg tgcctttgaa gttggtgcgg gtccgcaatc tgctgaatcc
      241 taatccttgc agcttctcta ccaattcctt aacggccgca gcaatcgtgg ggtgagtatg
      301 tccttcgacg gtcaaggctt catacatcac tttggatgaa tagccggacg gcgtccgatt
      361 gacgagaata gcgcgattaa agtagcgctc gctcggatcg actccttcga cctggtgttt
      421 ttcaacgaga ccctcttttt tcagcatgag cggaagcgag ctcatcacaa cctccttcga
      481 caccaggcaa ttgaggtcaa ccaacgcaat tataggggtg tgtccgtctc gagtgcaaca
      541 cgttgacaag gttccaaccc cctctctatc atccttctcg cttctgggga gtggccgact
      601 tgagtcggac gagcatgaat attcccaaca gtttgaccat tctccgcata ctcctgatcc
      661 ctgtctatat cgggttgttg gtgtatgaac aatatgacct ggcgctcttc gttctccttc
      721 tcgccggagt gacagacgca ctcgatggca ccattgcccg ggtgacaaat caacggacgc
      781 ggctgggtac gttcttggat ccgcttgccg acaagttgct gttgacgtca ggatttattg
      841 cgctttccgc cattcacctt attccgtcgt gggtcaccat tgtcgtggtg agtcgggatc
      901 tgatgctgtt attgggcacg gctgtcgctc aatttaccga gacacctatc gacatcacac
      961 cgacattttt aggtaaaggt acgacctttc tgcagttaac gtacgtcctg ctcgtcattt
     1021 ttttgagttc tcgacggatc gatctgtccc tcatcgaccc gctcctggct gggatggtcg
     1081 cgtttacact cctctccggg ctgcattatc tctatcgcgg atatcggcat tccaatacac
     1141 aaagcgcgta gttcttcttt gttgaggccg acgactagag tgactgcgtt gcgcgcagat
     1201 tgtgcatttg tttgacaggt ttttaggtcg ccagtagact cgctcaagag gtattccgcg
     1261 cggtttcaaa caggccggca gcccggccac atcgaaagac gagagtctcc tatggctaca
     1321 ttcgcatacg tcgggagaaa tcgatcgggt gccgtcaaga aaggggaatt gacggccaag
     1381 acgcgcgatg aggcagtcga tcaattgcgc aaacaaaacg tggttgtcac gagcttggaa
     1441 gaaagagccg ggaagggcgg cgggttcaat ctcaatagct taatggggtc ggggctgact
     1501 gataaggatc tggtggtgtt cactcggcag ttcggcacga tgatcaacgc gggtttgccg
     1561 ttagtgcaat gcttggaaat cctgtcgact caatctgaaa acaaagtgct ccgcgcgacc
     1621 gtcggcgaag tcaaaacgca cgtcgaggcc ggctcgacct tctcagacgc gctccgccgc
     1681 ttccccaaag tgtttgatga tctctatgtc aacatggtgc atgcaggaga agtcggcggg
     1741 ttgctcgaca cgattttgac tcggctggcc aagcacatcg aaaaagcgat gaagctgaag
     1801 gggcagatca agtcggccat ggtctatccg tcagccatta tcggagttgc ggtcatagtg
     1861 atttcagtcc ttatgatctg ggtcatccct atttttgcaa agatgttcaa cgaattgtct
     1921 ggtgggaaag taggattgcc gggaccgacg caactcgtca tcgacgtcag caacattttc
     1981 cagtcctatt ggtatgtgat gctcggcgtc attgtcacgc tcgtcttcgg catcaagaaa
     2041 tattacgcga cggctccggg ccggatgaat atcgacaggc tcttgctcaa gatcccagtc
     2101 gtgggagatt tgattcgaaa agcctcagta gccaaattta cacgcacgct cggcacgttg
     2161 atttccagtg gcgtcccgtt gttggaggga ctgttgatct gcgccaaaac gtccggcaat
     2221 aaggttatcg aagaggttct aattaatgct cgtgtgagta tcagtggcgg gaagaccatt
     2281 gccgatccgt tggcgaaaag cgaggtattt cccaagatgg taacgcacat gatcgccgtc
     2341 ggtgagtcca caggggcgct cgacgcgatg ctagggaaga tcgccgattt ctacgaagat
     2401 gaagtcgatc aggccgtggc gtccctgacc tcattgcttg aaccggtcat gatggtcgtg
     2461 ttgggcgttg tgatcggatt catcgtcatt gcaatgtatc tcccgatttt ccaaatggcg
     2521 aacgcgatcg gctagcacag agacggttag gcttcccgcc aagaaagcct gagacgtgtc
     2581 gctattcgac ccaggtacct gctcgtcagt gaggaaaggc aggtacctgc tacctgccaa
     2641 gaaaagtccg ttccaactcc tggcgaattc gacggtgtgt agcatgagtc acgtgcgatg
     2701 aaagacttgc gcaatcgatt gcattggttg atggccctac gcgtggccgt tgtcacgtta
     2761 ttgttggggt tctctcttgc attccaggcg tcgaacggtg agcgagtcga aacgttctac
     2821 acgcttatca ttgcaaccta cgccctcacg atcccttccg cgttgttact ccgatcgctt
     2881 tcaactccgt tagcccttac gacgttcttt tgggcccagg taggaatcga cttcgcgctc
     2941 gagacgatgt tggtggtcaa gacgggtgga gtcgaaagcc cgtttgccgt gttgtatgtg
     3001 atcacggtag cggtcgcgag tctcgttccg cgtcgtcgcg tcggagtcct cacggcctgt
     3061 tcttgcatga tcttatttgg gattgtgacg aacatccaac tctatggcct gctggagccg
     3121 tgggggtggc ttccgaaaag tcggctgacc ggtccagaaa cccttcaaac cttcggagcc
     3181 tatggattgg ccttcctcgt ggtcggcttc ctgagtggtg cgttggcgga ccaactgcag
     3241 caagcggatc agtcattgcg cgagaaggaa cagggactca cccgtctgca ggcttttcac
     3301 gagaatatcg tccaaagtat cagtagcggc gtctttacga cggatgcgtc gggaggcatc
     3361 acgtcgttca atccggccgc acaggaggcg actggttaca cactaagtca ggttttgggg
     3421 cgaccgtggc gcgaggtctt caattggcac cccaaccagc aatccgacga acggctggca
     3481 atgacgctgc cgacaatggt gcgcttcgat gtggaatgca agcgggcgga tggcaaccgt
     3541 ctgatcctgg ggatgacgct ttcgcccttg cacgagcagg gacaccagac aggattggtg
     3601 ggtgtgttca aggatctgac gcaaattcga gatctcgaag aggaaatgca tcgaaaggaa
     3661 tggctagcca gtttgggcga aatgtctgcc ggcatggcac acgaaattcg aaaccccttg
     3721 ggtgcattag ccggcgcgat gcagatgctc cgcaaggacg tgaccggcga cgagacgaac
     3781 catcggttga tggatatcgc gattcgagaa gccacgagac tcgacaccat tatcacggaa
     3841 tttctgcaat acgcccgtcc gccggcactc aatctggtcg agtgcaatct tcacaaaatt
     3901 cttgccgaga ctctggatct cgttcaacat gaagctcgga cacggtccca catcaagatc
     3961 attacttcgc tggcgaccgg acctttgacg gcgcaggtcg atcaggatca gttgaagcag
     4021 gtgttctgga acctgtccat gaatgcgttt gatgccatgc cggagggtgg gcagttgacg
     4081 atttcgaccg gatgccgccc gattgatatg ggagggaaaa agtgcgacgt gatcgagatc
     4141 gcgtttcacg atacggggga gggtatcccc aagcagcttc tggacaaaat tttcctcccg
     4201 ttctttacga ccaagaaaca agggtccggg ttaggtctcg cggctgtgca tcggattgtc
     4261 gatctgcacg gaggctggat caaggtggag agtcaggaac atcaagggtc tcgattcgtc
     4321 gtctgtcttc ctcgctccgg tggcgaaggc atgcggttgt ggcatgaggg taggacaccg
     4381 tggaaaaaat cttagtcgtt gatgatgaga aaagcttgcg ggaagtcttg agcatcatgc
     4441 tcaagcgcgc cgggtatacc gtcacggagg cttgcgacgg cgatgaagcg atcggccacg
     4501 tcaacaaaga gatctacgat ttggtcatca ccgacctccg catgccgaag gcagacggga
     4561 tggatgttct caaggccgtt aagtcctcgt caccggaaac cgtcgtgttg gtcattacgg
     4621 cctttgccac atcggattca gcggtcgaag cgatgaaaca aggcgcgtac gactacctca
     4681 ccaaaccgtt ccaggtcgac gaagtgcaat tgatcattcg caatgcgttg gaaaagcggc
     4741 ggctctccac ggagaacatg ctgttgaagc gcgaaatggc gagtcagtcg tcatttgcaa
     4801 aaatcgtcgg ccaaagtgag gccatgcaga aagtgtttga tgtggtcaag aaggttgcgg
     4861 acgcaaggag caatatcctc atctgtggag aaagcgggac cgggaaggaa ttggtggccc
     4921 gcgcgattca ctacaatagc gccaggagtc cgatgccctt tgtggctgtg aactgcagcg
     4981 ccgttccgga aacgctcttg gaaagcgaac tcttcggcca cgtgaaaggc tccttcaccg
     5041 gagccatttc caataaggcc ggtctctttg aggtcgccaa cggcgggact atctttctgg
     5101 acgaaatcgg cgacacgacg ccgacgattc aggtgaagtt attgcgagtg atccaggaac
     5161 gggagttccg acgcgtcggc ggtaatcagg atatgaaggt ggacgtccgt gtgatcgcgg
     5221 cgaccaatcg agatctggag aaagccgtgg cggagggggc gttccgcgaa gacttgtact
     5281 atcgactgga cgtcatcccg atccgactcc cgcctctgcg cctccggacg ggcgacatcc
     5341 cccttctggt cacctatttc ctggagaaat tctcgcaaga cagcgctaag ccggccccca
     5401 tgttgacgcc ggaggcctta cacgtcctcc tggaacacga atggcgaggc aacgtgcgtg
     5461 aattggagaa cctcatcgag cgtgttgtgg ccttctcgtc cgggaacccg gttaccgaag
     5521 cagatgtgcg cggctggctc catcggtccg tgacactaca acagcctggg atgcccacgg
     5581 acttgccgga cgacgggttg gatcttgagg cgctgatcaa cggcattgaa aaagatctgc
     5641 tgctgaaggc gctggagcgt gcgaagtggg tcaaaaagaa agcggcccga ctgttgagac
     5701 tgaacacgcg gtcgtttcgc tatcggttag agaaatatgc tattaaggga ggtcgtgact
     5761 aaggcgtcgc caccatcctc cgaactcacc attcttgctc ctgccaaagt caatctcatc
     5821 ctccgtattc tcgatcgacg ttccgacggc tatcacaacc tctggtcggt catgcagacg
     5881 gtggcgctgg aggacgaaat gcacatccgg cttcgtcccg gccatgagga tatacggctc
     5941 cgatgtaacg tatcggagct ggcgaccgat cgtaccaact tggtccatcg cgcggcatcc
     6001 gcagtgctgg cgcgagcagg gcaatcaagc gggatcgatg tcgacttgcg gaaacgcatt
     6061 cccatgggag ccggccttgg cggagggagc agcgatgccg cggcgaccat tatcggactg
     6121 aaccagttgc tgcaactcaa ttggtccatg acgcagatga cagaagttgg gcagtcatta
     6181 gggagcgatg tgtcgttctt tctttacggc cccacggcga ttgtatcggg tcggggggaa
     6241 acggttcggc caatgggcat agaaggcacc agatgggcgg ttctcgtcaa tccggggttc
     6301 ggcgtcgaaa cgaaatgggc ttataacgaa ctggcttcga ccagaggggg agttcgtcct
     6361 ctgggacgcc tccaccaaga gatcgatcat caacaacgct taacatggag tgaactctct
     6421 gccgcagccg aaaacgattt tgaatcaccg gtgttcgccg ctcatgaggt gcttcgcgag
     6481 atcaagcaga ctctcttgga ccaaggggcc gacatcgccc ttctgtcagg aagtggcgcg
     6541 acggtatttg gtctgtttgc taatgaggcc gaagcgcggc gggcggaagc ccatttctta
     6601 agggacaaca ggttgaaagt ctatgttgtg ttaacctgtt ccggtcccgt ggtgtgtcac
     6661 tgaagggcca gaaggattgc tgctctatcc gttgacaacc tgctgtcgtt tccgatacat
     6721 ttctcactcc agttggcttc ctcaacagcg cgtagcgaac gcggtaggcg ggttcgagat
     6781 cacgcccccc gacgcccgcc gtcgagacga accacgaacg aaatcagaac cttagagcct
     6841 gtgttccaga accgtccgag gggtcgatga acagagaatt gaaaatcttc tctggcaacg
     6901 caaacccggc actcgcccag gaaatttgcg cctatctggg gcagaaactt ggcgaggcca
     6961 cggtgtcgtc attcagcgac ggagaaatcc gggtcaagat cgaagagaat gtccgcggag
     7021 ccgatgtctt tgtcgtgcag tcttgctgcc agccggtcaa tgactcgctc atggagttgc
     7081 tcattatcat cgatgcgctg aaacgctcgt cggcaaatcg cattaccgca gtagttccgt
     7141 actttggcta tgcgcgacag gatagaaaag atcaaccgcg cgtacccatt tctgcgaagc
     7201 tggtggcgga tctcatcagc acggccggca ccgaccgtgt gttgacgatg gatctccacg
     7261 cgggacagat tcaggggttt ttcaacgtgc ccgtggacca tctctacgca ctcccggtgt
     7321 tgctggacta cattacgaaa aagaagttca acgaccttgt cgtggtgtcg ccggatgccg
     7381 gtggtgtgga gcgggccagg gccttcgcca agcgactcca ggccaacctg gcgatcatcg
     7441 acaaacggcg tgaagggccg aaccagacac agattatgaa catcatcggc gatgtggagg
     7501 ggaagagcgt cctgctgctg gatgacatga tcgatacggc cgggacgatc gttcagggcg
     7561 cgcaagcttg cgccgacaaa ggcgcacgag aagtctggac cgcctgcacg cacgcagtcc
     7621 tgacagggcc ggccctggag cggctgcaga aatcctgcct gaagcaggta atcgtcacca
     7681 atacgatccc gttacgaggg aaggaacaga cgtgcccgaa gctgcatcaa ttgtcggtag
     7741 ccccgttgtt gggcgaggct atccggcgca ttcatgagga tgagtcggtc agttcattgt
     7801 ttgcctgatc aagaacccac agataacgat aatcgagaga gggacggagg agtatcatga
     7861 aattcgattt agcggtgacg gtacgagaga aaagtggaaa gggggcggcg cgccaactcc
     7921 gacgtgaggg gaaagttccg gccgtgctct acggtcaagg ggagtgtctc ttagttacga
     7981 tcaatcccga cgcattgaat aagattctca agtcgcatgc gggaagcacc gcgttgattt
     8041 cactcgctat aaccggggcg aaagcaaagc ccaagcgcac ggccttactc cgcgactttc
     8101 aagtcgatcc ggtcgagggg cacgtactcc acgcggacct cttcgagatc tctatggaca
     8161 agccgattcg tgtgaaagta cctctgcatg tgatcggcgg tacgcccgct ggtgtgaaag
     8221 aaggcggtat cctccaccat aatatgcgcg agttgcacat tgagtgtttg ccgtcggcgt
     8281 tgcccgactt catcgaagtg gatgcctcgc cgctcaacat tggccagggg atccacttga
     8341 aggagattac caagaaagac ggcctgcgct atctggacga tcccgaccag atggtcgtga
     8401 gcgtggcggc gccaatgtcg gatgcgaagc tcgaagcgct tttgaccagt caagccgtgg
     8461 gaccggaagg ggcgaaggag cccgaagtgg tcgccaaggg gaaggcggca gccgaagggg
     8521 aagaaggagc cgcagcagcg cctggagcgg ccgcaccggc tggtgccgaa gccaagcccg
     8581 gcgagaaaaa ggaagccgcc gcggccgcag caccaaaggc tgagaaaaag gaacctgaga
     8641 agaagaagta acgttgcgcc tcatcgtcgg actgggcaac cccggtatag cctacgccaa
     8701 aacccgccac aacgtcggaa tgtgggtcgt cgaacaagcg gctgttcgat ggtcgatccg
     8761 tctcgccaca cgcggcacaa cccagcgcgg ttctgggcga ctgggctccg agttgctcga
     8821 actcgcgggt accctcgact ggatgaacgt tacaggccct ccgctgaaag gcctcctgcg
     8881 cgaattcaaa ttgaccgccg acgatctgat tgtcattcac gacgatctcg atctggagcc
     8941 ggggcgcctg agaatcaaac aagccggcgg tgacggtggc cataacggaa tcaagtcgat
     9001 catcgaggcg ctggacacac agcagttcgt gcgactgaaa atcggcatcg ggagacccgc
     9061 gccgcatcag gattcggccg actatgtatt ggaaccgttc acgaacgacg agctcgaggt
     9121 cctcaccccc tgtctcgatc gcgcggtcga ggcgctggaa tgtttgattc atcgcggaac
     9181 acctacagct atgaatcagt tcaatgtcag ggagaaaccg gctgaagagg acggggaaga
     9241 ggggtaacga aagtggggct ctgttgcgga atgatcgggt tgccgaacgt cgggaagacg
     9301 acggttttca acgccctcac aagcgggggg gcgctggcgg cgaactatcc cttcgccaca
     9361 gtcgatccga acactggtat cgcacttgtg ccggatccac gactccaaat ccttactgac
     9421 atttttcagt cgaagaagac cacgtacagc acacttgagg ttcgcgacat cgctggactg
     9481 gttgaaggtg ccagcaaggg cgaaggactc ggcaaccaat ttcttgggca catccgcgaa
     9541 gtcgatgcgt tgctacatat cgtccgctgt ttccagggaa ccgacgtggt gcatgtgagc
     9601 gggagcgtga atcccatccg tgacatcggc gtgatcgaga cagagttgat gttggctgac
     9661 ctcgacgcac tcgatcgccg caggcaaaag accgaaaaga aagttcgcgc gggggataag
     9721 aaggcggcct tcgaggtcga ctttctcacc cgtctcattg gactgctcga caaaggggaa
     9781 tggttgggca acttgacgta tacccccgat gaacggtcgc tcctcaacga atgccagctt
     9841 ttgtcggcca agccggtgct ctttgtcgcc aacgtctcgg aggggaagaa tgcggatgat
     9901 tccatggtga aaaccgtccg cgactttgcg gagaagcgag gggcacgagt cgtgaccatc
     9961 tgcggtcagc tcgaagcgga gttatcctcg ttgccggaga gcgagcgagc cgattttctt
    10021 aaggaaatgg ggttgactga gtcggggttg gtccggctca cacgcgaagc gtacactttg
    10081 ttgaccatca tcaccttctt taccgccggc gaaactgagt cacgtgcctg gcccattccg
    10141 aaaggtatga aggcgccgca agcggcgggc aagatccact ctgacatgga gcgaggcttc
    10201 atccgcgccg aggtctatca ctacgatgac ctcatggcgt gcggctcgga ggccaaggtg
    10261 aaggagaaag ggttgttccg gctcgaggga aaagattacg tgatcaaaga agcagacatc
    10321 gtgtacttcc gcttcaatgt atagcggcca ccacccacca ttaccaagta gcggacagat
    10381 gagaatacac ggtccatggt ctatgttgac agttttgtga ggcccgtgat accgtccacc
    10441 gttcgttgcg ccaaaatagg cgctcctaca cctcgctctc aacgtgtttg agggcctttg
    10501 tccaggagga ttccttcatg gagctctacg agtctctgtt tattatccgt ccgtccgtct
    10561 ccgatgagga gacgaacgcg ctcatcgaga agatgaaggg cgtcgccgac aagactggcg
    10621 cgcagttcat caaagccgaa aattggggtc gcaagaagct cgcctatgaa gtcaggcggg
    10681 aacggaaggg cacgtacgtc tacttttact tcaaggcgcc caacaatacc gtgggagaac
    10741 tggagcgcgc ctaccggctg gaagacaaca tcatcaagtt cctgaccgtg catcttgaga
    10801 aggagttggt gccgcggcgg ccgatggagg ccccgtcaga ggagtttgcc ggtggccgga
    10861 tttaacaaag tcatcctcat ggggaatctg acgcgcaatc cggaattgcg ctataccccg
    10921 agcgggacgc cggtggcgag cttcggcttg gcgacgagcc gacggttcaa acagggtgag
    10981 gaattgaagg aagaagtctg ttttgtcgac atcgtggtgt tcgggaagca ggccgaacat
    11041 tgcgggcaat atctcagcaa gggcaatggg gcgatcgtcg aaggcaggtt gcagcagcgc
    11101 cgctgggaaa cggaagacgg tcaaaagcgg agcaagcatg aggtcgtcgc gcagacagtc
    11161 acgtttatgc cgaagcgcag cgaggccagc ggtggaggtg agccgtcgat gccggaggat
    11221 gtgagttttg aatctgagga acatgcctaa ctgaggagac tcgtcatgga tcgtgaacgt
    11281 gggggagatg gtggcggccg agaaggcagc gggggaagcg gacgattttt ccaacgccgc
    11341 cggccctgcc ggttttgtct cgataagggc tcgattgatt tcaaagacgt tgggctgttg
    11401 cgaaactttc tgaccgagcg cgggcggatt gttccacggc gcatttccgg aaattgcctg
    11461 ggccatcagc gcgagctgac ggtggccatc aagcgggcgc gtcacatcgc cctgatcagc
    11521 ttcgcagaag aacggtaaga ggcatgttcg ggatggattt gttgataggt gatgcgatgg
    11581 acctcctcac accgaagatc gctgatattg ctgcggcggg gctgtcgttg tccggtgaag
    11641 tgaccgccga agaactgggg ttgagtgagg ccgatgccgt tgtcaaggca ccgcttgcgg
    11701 tgacgatgga tctggccaag gccgatgggt tgattaccgt gaccggcgtg gtggaaggca
    11761 cgatcgttcg acagtgtgtg cgatgcttga gagaatatga cgatccgctc gcattttcca
    11821 ttcgagcggc ctatgcgccg gatgtcaaac cggtccagcg gcgtcccaag caggcggaca
    11881 tgagaaaaac gcagctggtt cccgtcgaag cggaagagga ttcagaggag gaaccagacg
    11941 atcaatatca ttaccagggt gaccacgtgg aattggcgcc gatggtgcgc gagcacgtga
    12001 tcctggcagc gccgatgcag ccgttgtgca aagaggattg cgcaggactc tgtgcctcat
    12061 gtggaaaaga tttgaacgag gggccctgtt tctgtgcggt agatccaccg gtcacaacat
    12121 tccgagtggt ccggaacacg aaacaacaga ccggtgatca cggagcttct tagtcagccg
    12181 tgttcagccg tcgtaaggag cagccatgcc aaatccgaaa cataaacatt cacgagccag
    12241 gcgcgataag cggcgaacgg ccaagttgcg catgacaccg ccgggcatgt cggtatgccc
    12301 gcaatgccac gaattgaa
//
LOCUS       DATDNW010000117         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1309187, whole genome shotgun sequence.
ACCESSION   DATDNW010000117 DATDNW010000000
VERSION     DATDNW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309187"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VI359_02955"
     CDS             <1..198
                     /locus_tag="VI359_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973243.1"
                     /translation="PARDGMDVTLELTREFLDAKVIAMTGATGDRNFLSIAKLFGARE
                     ILQKPFTVDEVRRLVRYTLKH"
     gene            complement(307..1797)
                     /locus_tag="VI359_02960"
     CDS             complement(307..1797)
                     /locus_tag="VI359_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY6973244.1"
                     /translation="MILGFVILYLLISVGIGLYAATRVHSAKDFAVAGRSLPLPIVTA
                     TVFATWFGAEAVLGMPATFVKDGLRGVVADPFGASLCLILAGLFFAPRFYRLNLLTVG
                     DYYRIRYNRTVEVLCTLCVVVSYLGWVAAQFKVLGLVLNVVTNGGVSQKLGMVIGAGI
                     VLTYTTFGGMFSVAILDFVQLGVILGGLLYIVSVISDLTGGVGTVIAHAAQAGKLEFF
                     PSAKLSAWIPFIGAGITMMLGSIPQQDVFQRITSAKDERTAVRGSVLGGGLYFAFCFV
                     PMFLAYAATLVDPAKFGTLLEQDSQLVLPTLILQHTPIVAQILFFGAVLSAVMSCSSA
                     TLLAPSVALSENVIKCALPSMADRQFLRMMRFVLVGFACVVLTIALSSNSSIYMLVVN
                     TYKVTLVAAFVPLCAGLYWPKATTQGALCAIVAGLTTWIGCEFFGSSDSVWPPQLVGF
                     LMSIAGMVIGSLLLPLGGSPLMRPIVDPAPRVASHTMPLTTVDLHD"
     gene            complement(1808..2356)
                     /locus_tag="VI359_02965"
     CDS             complement(1808..2356)
                     /locus_tag="VI359_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY6973245.1"
                     /translation="MVRLWWLLIFVMLTTQGWAAEFQLSSPTIKNRGKIGHEHVFAGF
                     GCSGGNVSPALQWQGAPKDTKSFALTMYDPDAPTGSGWWHWVIFNLPPTLNSLPANAG
                     KPDGGTAPAGSIQSITDFGHPGYGGPCPPAGDKPHRYIFTLYALKLDQVPLKSDASGA
                     MVGFYLKQNMIAKASFTAFYGR"
     gene            2556..3062
                     /locus_tag="VI359_02970"
     CDS             2556..3062
                     /locus_tag="VI359_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973246.1"
                     /translation="MTQNIAHRLNPLPDTSTPARQVMAGIIGLGILLLTAIPADANDP
                     QGPWECSGYSGEAHTRCLAAFVEAQREQIASLQGKVQAQQETVNQLKAQMDRQAAAHA
                     SSQSQVAPPAIIPTVPPLYGYPPLYGYPPVGIGVYIGRPWFYGPPYFYRPYFYGPRYF
                     GYRHWGHR"
BASE COUNT          813 a         1002 c          881 g          603 t
ORIGIN      
        1 ccggccagag acggcatgga cgtcacgctg gaactcacgc gcgaatttct cgacgccaag
       61 gtcatcgcca tgaccggggc caccggcgac cggaactttt tgagcattgc caaactgttt
      121 ggagcccgag agatccttca aaaaccattt acggttgacg aagtccgtcg tctggtccgc
      181 tatacgttga aacattgacc accgaaagcg gccctcttcc tcccgtaatc tcttcacttc
      241 catgacaacc agggtccagc ctcgtccaga ggtggaagca atgctgagca acaacgttcg
      301 ccgcgtttag tcgtgaaggt cgacagttgt gagtggcatc gtgtgtgagg caacacgggg
      361 ggcagggtcg acgatcggtc gcatcaacgg agagccgccg agcggaagca acaacgaccc
      421 aatgaccatg cccgcaatgg acatgagaaa accgaccaat tgcggaggcc atacggagtc
      481 tgacgagcca aagaactcac acccgatcca tgtcgtcaga ccggccacaa tcgcgcagag
      541 tgccccttga gtggtcgctt tcggccagta gaggcccgca cataagggaa cgaaggcagc
      601 taccaacgtc accttgtatg tattcacgac aagcatgtag atactcgaat tggaactgag
      661 cgcgatcgtc agcaccacgc acgcaaatcc gaccaatacg aaacgcatca tccgtaagaa
      721 ttgcctgtcg gccatgctag gcagcgcgca tttgatgacg ttttcgctca gcgcgaccga
      781 cggggccagc aacgtcgcgc tcgaacaact catcaccgcc gacagcaccg cgccgaagaa
      841 caggatttgc gcgacgatcg gcgtgtgctg aaggattagg gtcggcagga cgagttgcga
      901 atcctgctcg agcagcgtac caaactttgc ggggtcaacc aacgtggccg cataggcaag
      961 aaacatcggg acaaagcaga acgcaaaata gagccctcca ccgagcaccg agcctcgcac
     1021 cgccgtccgt tcatccttgg ccgacgtgat acgctggaac acatcctgct gcgggatcga
     1081 acccagcatc atcgtgatac cggcgccaat aaacgggatc cacgcactca gcttcgccga
     1141 cggaaagaat tccaattttc cggcttgagc agcatgggcg atgactgtcc cgacgccacc
     1201 ggtgagatcg ctgatgactg agacaatata gagcaatccc cccagaatga cgccgagctg
     1261 aacaaagtcg agaatcgcga cggaaaacat gccgccgaac gtcgtatagg tcagcacaat
     1321 ccccgcaccg atgaccatac cgagcttctg actcacaccg ccgtttgtca cgacattcag
     1381 caccaatccc agcactttga actgggccgc cacccaacca agataggaga ccacgacgca
     1441 tagcgtacac agcacttcca ccgttcggtt gtagcgtatc cggtagtagt ctccgacggt
     1501 gagcaaattc aagcgataga aacggggcgc gaaaaacagc ccggcaagaa tcaaacagag
     1561 gctcgccccg aatggatcgg cgacgacgcc gcgcagtccg tccttgacga acgtcgccgg
     1621 catgcccagc accgcttccg caccgaacca agtcgcaaac accgtggcgg tcacaattgg
     1681 aagaggcaac gaacgaccag ccaccgcgaa atccttcgca ctgtggacgc gcgtggccgc
     1741 gtagaggcca atccccacgg agataaggag atagaggatg acgaatccca gaatcatgca
     1801 gacgggctca acgcccgtag aaggccgtga aggacgcctt ggcgatcatg ttctgcttga
     1861 gataaaagcc caccatcgcg ccggaggcgt cactcttcag aggtacctgg tcgagcttca
     1921 acgcatagag ggtgaagata tatcgatggg gcttgtctcc tgcgggcgga caaggaccgc
     1981 catacccggg gtgaccgaag tccgtgatgc tctgaatgct gcctgccggg gcggtcccac
     2041 cgtccggctt tcccgcgttg gcaggcagcg agttcaacgt gggaggcaga ttgaaaatga
     2101 cccaatgcca ccagccactg ccggttggcg catccggatc atacatggtc aatgcgaaac
     2161 tcttggtgtc tttcggcgcc ccttgccatt gcaaagcagg tgacacgttt cccccgctac
     2221 agccgaatcc cgcaaacacg tgttcgtgac cgatcttgcc ccggtttttg atcgtcggac
     2281 tgctcagctg aaactcagcg gcccatccct gcgtcgtcag catcacgaat atcaatagcc
     2341 accacaggcg aaccatgatt tgcccctttc ggaaaagaga acattacgga cgcgtgatca
     2401 caagaaccga cgacagatgc gggaggattg tatcgagaga cgaaggaaca cgcaacgtga
     2461 gaaacagctg aaatcaaagc ggcgatgtgc cccctgtcat cttggttttt ctcgtggtag
     2521 cttgtaagta agaggcccaa caaggaggga agcccatgac acagaacatc gcccatcggc
     2581 tgaacccttt gccagatacc tcgacgcccg cccggcaagt gatggcggga atcatcggcc
     2641 tgggaatcct cctcttaacc gccataccgg cagacgcgaa cgacccgcag ggaccatggg
     2701 aatgttccgg atattcaggt gaagcccaca cccgttgcct ggcggcattc gtggaagccc
     2761 aacgcgagca aatcgcatct cttcagggga aagtgcaagc ccagcaggaa acggtgaacc
     2821 aactgaaagc ccaaatggat cgtcaggcgg cggcccatgc gagcagtcaa tctcaagtcg
     2881 cacctcccgc catcatcccg acggttcctc cgctctacgg gtatcctcca ctgtatggat
     2941 atcccccggt cgggatcgga gtctatatcg gtcgcccgtg gttctatgga ccgccgtatt
     3001 tttatcgccc ctatttttat ggcccgcgct atttcggcta ccgtcactgg ggccatcgct
     3061 gatcggagcg tcaagactac tcacgttcgg tcgtctcacc atcgcaaagc ataattgtct
     3121 tgaggacaac ggttatatcc cgtactctac gaaacttcac actcatgacg atcatgatca
     3181 tccattcaca tccatcagaa agggctactc atgcatgtct tgctttcagc tctgatgctc
     3241 tggttctgcg ttggactccc ctccgccttt gcggccgctg aaccgacgaa cgacgacca
//
LOCUS       DATDNW010000118         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_284710, whole genome shotgun sequence.
ACCESSION   DATDNW010000118 DATDNW010000000
VERSION     DATDNW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284710"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VI359_02975"
     CDS             <1..336
                     /locus_tag="VI359_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6973247.1"
                     /translation="LIAHMRLPVVVVGRSRLGGINHALLTVEALRRRKIPIVAIVLNQ
                     TEPGRSKLDRLQERTTVEILRKEAGVPVLGPLPYRSELSRQFRRSVTRLAQSTAIKKL
                     AKIVTRAAR"
     gene            complement(317..955)
                     /gene="thiE"
                     /locus_tag="VI359_02980"
     CDS             complement(317..955)
                     /gene="thiE"
                     /locus_tag="VI359_02980"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY6973248.1"
                     /translation="MTVNRHRRALHGLYIILDPSICPDRPLHSVLTQSAQAGTQIFQY
                     RNKLVSMKEAYAEALPLRNAAADLGVLFIVNDRCDLALAVEADGVHLGQDDLPYAEAR
                     MLLGPGKIIGLSTHNAEQVRQADKLKPDYIGFGPIFTPGSKEDHDPVVGLEGLRQVRP
                     LTKLPIFAIGGIHAEQACAVMQAGADGIAVISAVLEASDVKKAVGQLISLLS"
     gene            complement(1020..2360)
                     /gene="accC"
                     /locus_tag="VI359_02985"
     CDS             complement(1020..2360)
                     /gene="accC"
                     /locus_tag="VI359_02985"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY6973249.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAVHSEADASSLHVRA
                     ADEHICIGPPEAALSYRNIPNVLSAAEITGADAIHPGYGFLSENAHFAEVCESIGIKF
                     IGPTSENIALMGDKAKAREIVARKGLPVTPGSPGELKSEQEALDAATKIGYPVIIKAS
                     AGGGGRGMRVVNKADDLARAFQAAQAEAKSTFGHDGVYLERYFLEPRHIEVQIIADHR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAVDEKLRKEIGRVAVEAVKAVHYRNVGTVEF
                     LLDKDHNFFFMEVNTRIQVEHPITEMVTGIDLIKEQIKLANGQALSFKQQDIRLNGHS
                     LECRINAEDPDKFTPSPGLITKYNAPGGFGVRVDSAMESNAMVAPHYDSMIAKVITHG
                     RDRQEAIARMRRALDEFVIEGIKTTIPLHRRILNDPDFQKGHVSTTFLDRFLAG"
     gene            complement(2394..>2962)
                     /gene="accB"
                     /locus_tag="VI359_02990"
     CDS             complement(2394..>2962)
                     /gene="accB"
                     /locus_tag="VI359_02990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY6973250.1"
                     /translation="KKRARKQRANKPPIILPQDFADIPIGQGKSFLLGNQSKHVQELI
                     DILKRNNLTELELEREGVRIRVRHEQGIKTITSTVADASSGTVASASQPSPTGSTSAE
                     DVTGLVTIASPIVGTFYRSPSPDADPYVEEGDYVKKGQVLCIVEAMKLMNEIESEVDG
                     RVTKILAESTKPVEYGQALFLVDPKATP"
BASE COUNT          604 a          804 c          826 g          728 t
ORIGIN      
        1 ctcatcgccc atatgaggct tccggtcgtg gttgtgggtc gatctcgact gggcggtatc
       61 aaccacgcgc tactgactgt cgaagcgctt cggcgtcgca aaatacccat cgtggcaatt
      121 gtcctgaatc aaaccgagcc ggggcgatca aagctcgatc gcctgcaaga acggaccacg
      181 gtcgagattc ttcggaagga ggcgggggta ccggtgctcg gtccactccc ctatcgatcg
      241 gaactgtcac ggcagtttcg gcgatcagtg acccggttgg ctcagtcaac ggctataaaa
      301 aaactggcaa agatcgtcac gagagcagcg agataagttg cccgactgct tttttgacat
      361 ccgatgcttc gagcaccgcc gagatgactg caatgccatc ggcgcctgcc tgcatgacag
      421 cacaggcctg ttccgcgtgg attcctccga tcgcgaagat ggggagcttc gtcaacgggc
      481 gaacttgccg caacccttcc agcccaacca ccggatcatg gtcctccttg gagcctggcg
      541 tgaagatcgg tccgaacccg atgtaatcgg gcttcagctt gtccgcttgc cgcacctgct
      601 cggcattgtg agttgacaag ccgatgattt tgcccggtcc caagagcatc cttgcctcag
      661 catagggcag atcgtcctgg ccgagatgca ccccgtccgc ttccaccgcc agcgccagat
      721 cgcaacggtc attgacgatg aacagcaccc ccagatcagc tgcagcgttt cttaatggca
      781 atgcctctgc gtaggcttct ttcatcgaga cgagtttgtt gcggtattga aagatctgtg
      841 tgccggcttg cgccgattgt gtcagcacac tgtgcagtgg tcggtctgga caaatcgacg
      901 gatcaagaat gatataaagt ccgtgcagtg cacgtctatg cctattcact gtcatgatct
      961 gagcatgtat cgtcaaaaaa agaagttaac tccaggcgcc gaattcggtg agaagctgat
     1021 cagccagcca ggaatcggtc gaggaatgtg gtcgagacat ggcctttttg gaagtcagga
     1081 tcgttgagga tgcgtcggtg aagcgggatc gttgtcttga tgccttcgat cacaaattcg
     1141 tcgagcgcgc ggcgcatgcg ggcgatggct tcctgccggt cgcggccgtg ggtgataacc
     1201 tttgcgatca tggaatcata gtgcggggca accatcgcgt tggattccat ggcggaatcg
     1261 acacggacac cgaacccgcc cggtgcgttg tatttcgtaa tcagccctgg cgagggcgta
     1321 aatttgtccg gatcttcggc gttgatccgg cattccaggc tgtgaccatt taagcgaatg
     1381 tcctgttgct tgaatgagag cgcctgtcca ttggccaatt tgatctgttc cttaatgagg
     1441 tcgatgccgg tgaccatctc ggtgatcggg tgttcgacct gaatgcgcgt attcacctcc
     1501 ataaagaaga aattatggtc cttgtcgagc aagaactcta cggtgccgac gtttcggtag
     1561 tgcacggcct tgacggcttc gacggccaca cggccgattt ccttccggag cttttcgtcc
     1621 acggcgggag acggcgtttc ttcgaccagt ttctgatgcc ggcgttgtat agaacagtct
     1681 ctctcaccca agtgaacgac gtgaccacga tggtcggcaa taatctgcac ctcgatatgt
     1741 cgaggttcca ggaaataccg ttcgaggtag acaccgtcat ggccgaaagt cgatttggct
     1801 tctgcttgcg cggcctggaa tgcgcgcgcc aagtcatcgg ctttattaac gacccgcata
     1861 ccacgtccac ccccgcccgc agaagcctta atgatgacgg gatacccaat cttggtcgcg
     1921 gcatccaacg cttcctgttc gcttttgagc tcgccggggc ttcccggtgt gacgggtaag
     1981 cctttcctcg cgacgatttc tctcgccttg gctttgtcgc ccatcagggc gatgttctca
     2041 gacgtgggac cgatgaactt gatgccgatg gattcgcaca cttcagcaaa gtgtgcgttc
     2101 tcagacaaga acccatatcc tggatgaata gcatcggcgc cggtgatttc tgccgcgctc
     2161 aaaacgttcg ggatattacg gtagctcagc gctgcttcag gcggcccgat gcagatgtgt
     2221 tcgtctgccg ctctgacatg gagtgagctg gcatccgcct cagaatggac cgccacggtc
     2281 ttgatgccga gttctttaca ggcccggatg acccgtagtg cgatttctcc tcgattggcg
     2341 acgagaactt tcttgaacac gccgtagctc cgagttggat tagataaagc gaattagggt
     2401 gtggctttcg gatcgacgag gaagagcgcc tgaccatatt cgaccggctt tgtgctttcg
     2461 gccagaattt ttgtcactcg cccatccacc tccgattcaa tttcattcat cagtttcata
     2521 gcttccacaa tacacaggac ctgacctttt ttcacgtaat ccccttcttc tacgtaaggg
     2581 tcggcatctg gggaggggga gcggtaaaat gttccgacga tgggagacgc aatcgttacg
     2641 aggccagtta cgtcttcggc cgatgtgctc cctgtggggc ttggttgaga tgctgacgcg
     2701 acggtaccgg agcttgcgtc ggcgacggtg gacgtaattg tttttatccc ttgttcatgt
     2761 cggacacgaa tcctgactcc ttctcgttcg agttccaact cggtcaaatt atttcgcttt
     2821 aatatgtcga taagttcctg gacgtgcttg gactgattac ccagcagaaa ggactttcct
     2881 tgcccgatgg ggatgtcagc aaaatcttgc ggaagaataa tggggggctt gtttgctcgc
     2941 tgcttgcgag cccttttttt ac
//
LOCUS       DATDNW010000119         7664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_426458, whole genome shotgun sequence.
ACCESSION   DATDNW010000119 DATDNW010000000
VERSION     DATDNW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426458"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..781
                     /locus_tag="VI359_02995"
     CDS             584..781
                     /locus_tag="VI359_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973251.1"
                     /translation="MHYVRIGKKIVNLDSVSYCEAQIWQDAMSVKMFFAGSAHNTPLV
                     FSEDEAKELWKYLEYVAEKPV"
     gene            complement(924..1607)
                     /locus_tag="VI359_03000"
     CDS             complement(924..1607)
                     /locus_tag="VI359_03000"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY6973252.1"
                     /translation="MNMTHNENNKAKTVQSHSLCLATGLLVVGVIIALFAPSVLADAQ
                     VYEYVSPTGIVNLTNVPANTRFKEFRSPARYHRAVSDMELEQAVALAARQHKVAPALL
                     LAVMKAESDFNPMVISKAGAVGLMQLIPETAIRHGVRNLYDTEENIVGGAKHLRYLLD
                     RFHGKIRLALAAYNAGERKVDRYGKIPPYKETQLYVEKVMGYYREFKKGIWIVPIAVT
                     TSADPRRGS"
     gene            2956..3333
                     /locus_tag="VI359_03005"
     CDS             2956..3333
                     /locus_tag="VI359_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973253.1"
                     /translation="MEDLVIEEEGTCSALKITLGDFLVFINWMNQTEKALDRGEMLPA
                     VPDLDSGKVKGWIQLVQRLEERFRRSEEKKLALEANVIAQEAQLGQLLSHLHANIASL
                     YENLGQSQKAEEVRRRAEALHVG"
     gene            3638..4585
                     /locus_tag="VI359_03010"
     CDS             3638..4585
                     /locus_tag="VI359_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HEY6973254.1"
                     /translation="MIKVLLVEDNDVDARLTQDLLAEWSVEEFQVVHVITLAEGLARL
                     TGERFDAVLLDLSLPDAFGLPTVRQVHATSPSTPVVVLSGVSDQSLALQAVQQGAQDY
                     LVKGQGHPELLARAVRYAIERKRTEERLIYLAQYDHLTGLVNRTLFRDRLFQGMARSK
                     RLQQSLGLMLLDLDRFKMVNDTMGHDIGDELLKSVADRLKCCVREVDTVARMGGDEFT
                     IILEGVASVENVTVVAKRIVEAIAASFEIKNRQISIGVSIGITIYPHDDKEIDDLLKH
                     ADDAMYQAKQEGGSGFRFYGSNEPQTPDLYSQFSQTPRT"
     gene            complement(4657..5469)
                     /locus_tag="VI359_03015"
     CDS             complement(4657..5469)
                     /locus_tag="VI359_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY6973255.1"
                     /translation="MLVEQKPIPKTDPPTNPLGGKTLVVDAQDVSAYARPSAALNDAG
                     ETDQVFIRPGTYEDKIFVSGRPILMIGAGRDQVQIFSRRGGPLYLQRVPGGRISGITF
                     RYVGSDQNSAMNLMDSSCTVTQCRATDGILSGVVIYGPQSRTAFIDNEVCHNRESGVF
                     VFAGAQPRLADNRCFGNHHFGIAVRDPGSHPELVRNHCRENLLSGILLFHHAEALLID
                     NTCADNNQWGIVLTPDCHPTPDAADFEQSNVFMPNPRGRVCVTEKPLADIGR"
     gene            5807..6304
                     /gene="ybgF"
                     /locus_tag="VI359_03020"
     CDS             5807..6304
                     /gene="ybgF"
                     /locus_tag="VI359_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6973256.1"
                     /translation="MMNRLKHPLLVTAAVLFSFNLAVAASPQLQDTSRHLYDRVMEEF
                     KHKDYEAALAGFRFFLELHGQSSLAANAQYWIGECHYRLGHYKDALKSFYDVVSYYPL
                     SPKLAASSLKIAQTYTKIKDHEKARMMYERVVDQYPDSAEAEIARKALEAAAARDEPT
                     TLPTE"
     gene            complement(6339..6848)
                     /locus_tag="VI359_03025"
     CDS             complement(6339..6848)
                     /locus_tag="VI359_03025"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEY6973257.1"
                     /translation="MDWGLIGLGIGVALLVWWFAVPSPSIKAKTGTFVMPPHVTLQPQ
                     PLLTDSELFLYNLMRLAVQDQYLVFTQVPLWSIVHVEAEGAIRSDLLHRIALKRLDFV
                     LVHPGSRLVEQVVQLEDDQPSHSRQGDRQEIVKAVVESAGIRFTTLPAHTSYTIPQLA
                     TLLGLGDSD"
     gene            complement(6857..>7664)
                     /locus_tag="VI359_03030"
     CDS             complement(6857..>7664)
                     /locus_tag="VI359_03030"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY6973258.1"
                     /translation="AVIAERTGITTIANFRPRDIAAGGPGAPLAPMAHALLLKHPRRG
                     RLIVNLGGISNVTYVPPGGGQGGVQAFDTGPANMVLDSLIVKLTAGRLLMDREGKLAS
                     KGRVNDRLLGKLLAHPFLSKRPPKSTGREEFGPHLMEDLITAQQQLELSVEDLLATCS
                     MWTAKAVGTARRWIHGKIDEVVVGGGGVRNRAIMAHLATVFDPVRVTTFEALGWDSKA
                     FEAVAFAILAYQTATGQWGNIPAVTGASHRVLLGTIVPGSPQWREQLHSR"
BASE COUNT         1828 a         2038 c         2026 g         1772 t
ORIGIN      
        1 tgacgaggat tatgctacgt ttttcaaacg agacccggag cataagggga tgctcctgat
       61 ggtcagtccg caggatggca gagtccaagc gattgtggag gtctgccaga agctgaaaaa
      121 ataggttagg tgccgagtgt gcggaagtga ttctcgttgg tcgtgaccac tgtgctaaat
      181 ttgtgctaaa agttatccaa aatgaagtga actaatagaa ataactgata cagatggaac
      241 gctgctaatc ctttgcaatc actaataaaa gcgagaaacc agacgaaaga tcagagtgtt
      301 aagaaactat cgcttaacgg cctcataacc caaaggtcga aggttcaaat ccttcccccg
      361 caaccaaaac catccctcca cgataattcc tcctgtttat acacaaaaag tcctgcctcc
      421 gctcggacag gtggacggtg cactttgtac agcttcatat catcgcccct ccttttgcgt
      481 tcccggatct gtcacaagac aacacacgaa agtagttagt cattggatag atactgcact
      541 gatggtatag tccgaacggc atgcgataag tagagaggtg ctgatgcatt atgttcgcat
      601 cgggaagaag attgtgaatc ttgatagtgt gagctactgc gaagcgcaga tctggcaaga
      661 cgcgatgagt gtgaagatgt tttttgcagg gtctgcgcac aacaccccat tagtcttcag
      721 cgaggacgaa gcaaaagaac tctggaagta tctggagtac gtggcagaga agccggtgtg
      781 acagcgcccg ccctttttta ctcagacgcg accttcacct tccccttatt cgtgagttcg
      841 atcgagacca ttctatacgc gtttgaatcg cttcgttagg cgaacagttt cacgagttgc
      901 aatctgttac gactagcaca gattcaagat cctcgcctcg gatcggctga ggtggtgacg
      961 gcaatcggaa ctatccagat gcccttcttg aattcgcggt agtatcccat gaccttctcg
     1021 acatagagtt gtgtttcttt gtacggcggg atcttgccat accgatctac cttacgttcg
     1081 ccggcattgt acgccgccaa ggccagccga atttttccat gaaaccgatc cagcagatac
     1141 cggaggtgtt ttgccccgcc aacgatgttc tcttcggtat catagagatt acgcacacca
     1201 tggcgaattg ctgtttctgg gatcaactgc atcagcccga ccgctccggc tttggagata
     1261 accatgggat tgaaatccga ctcggccttc atgacggcaa gaagcaacgc cggcgcaact
     1321 ttatgttggc gggcagcgag tgcaaccgct tgctcaagct ccatatctga cacggccctg
     1381 tggtaccgag ccggcgatct gaattccttg aagcgggtat ttgccgggac gtttgtcaga
     1441 ttcacgattc ccgtcgggct cacatattcg tacacttgcg cgtcagccag cacagatggt
     1501 gcaaataacg cgataatcac cccaacgacc aggagcccag tagcaagaca caggctgtgt
     1561 gactgaaccg tctttgcctt gttgttctca ttgtgagtca tattcatttg tcttccctcc
     1621 ctaccaccgg atttcatgat cccctgccgc cgtctccggg tgtttctcgt tcgggaattg
     1681 actcagtcct gccatgctac tagcgcagtt tttatgcctt ttatctgacg acggaaattg
     1741 caatattatc ctattaatac aatatgttat atgctctcat cgcccccaac ttttgtagga
     1801 gtagcagggt ggggttattc catttttcga cttcttgagg cgcattttgt tgtcctttcg
     1861 cgggatggca tatcagcgga cggctatgct agggtggatt gagtcgatga tgacaataag
     1921 gacttattag agatgatcaa aatccgatat tgccctcgcg tacattcgag ttttcctgtc
     1981 gtcttcacta gtgaaagctg cgtgggggaa ggaaccgtcg tgaacgtttc cgtgcccggg
     2041 tgtgctatcc agagcaggag gcgagttcag gccggttctt acttggagat gagaatgctg
     2101 gtaccagaca cggcttctcc actccacata gggttggcta aggttcgctg gtcagaagga
     2161 cgccgcttcg gtgtcgagtt catccgtatg cccggcgggg atcagattcg tcttggccgt
     2221 ctggtcaaaa tgaaggttca tttcgaatct ccctcaccat ccagtagatg gcgtggcgca
     2281 cagtgttcag gtagtcgccg acctagctga tagcagaggc ttagatgggg aagatccttc
     2341 ttactattct gctgctgtct ctcagtggtt gtgctgaacc ctatacagtc ttccacagta
     2401 aaacggggga tccgatttta attggccggc gtgctttctc gtatgatgga tgcctagaaa
     2461 cgatgcggga ggaagccgct cgccttggcg tcacgtttcg cactgtgcag gtaaagggtt
     2521 cgacagtagg gcggtcgctg ctctggccat ttgaaccagg cttcgcctgc gaagccgcca
     2581 ttggaccgga gcgcccgccg cacggtgcct accccaacgt tccccctctc gctcctcaag
     2641 ggtaatcaac acctctctcg gtcgattcgc taagttcccc atcggaaaaa tttgcattgg
     2701 aattcctgtc gaattcggcc ataagagacc agttgacaat acaacccctg gcgcagtatt
     2761 actcgcgcaa caccaatctg atgtggctaa cggctcgatt gtctgcgaac atatccatct
     2821 tcatttttca aacaacagaa gttcaacaag gaggtgatcc atactgcgag agaggtgtaa
     2881 tgcagcacac agcaacatca gaaattgggt ggaggccgtt cgcaacaact gtagaatcgt
     2941 ctggtaagga gacatatgga agatttggta atcgaagaag aaggcacgtg ctccgcactt
     3001 aaaattacgc tcggtgattt cctcgtcttt atcaattgga tgaaccaaac ggaaaaagcc
     3061 cttgaccgtg gtgaaatgtt gccggcagtt cccgacttgg attcaggcaa ggtaaagggt
     3121 tggattcaac tcgttcagcg gttggaagaa cggttccgcc ggagcgagga aaagaagctg
     3181 gcgttggaag ccaacgtcat cgcacaggaa gcgcaactgg ggcaactctt gtcccattta
     3241 catgccaata tcgcctctct ttacgaaaac ctcgggcagt cccagaaagc tgaggaggtt
     3301 cgccgtcgag cagaagcgct tcatgtaggc tgataaaccg actgccgctc gccccggcaa
     3361 gagccgaagc tctcctcgtc aatagtcgac cgcctgcttg aaagctgagt gggtggaagg
     3421 tatggtgtgt cctcaccata gggaccgcca acggcactgg aaggcttgtc ttcgtgggcc
     3481 atgggttcta ctgcctgccc aaattactca tttccttggt cgcacatgac ctcatgttta
     3541 taataaaaac tgcacaggct gaatggtcta gcgagcgaga ggaacaagca atcgggcgcc
     3601 ggagtaggtt tctacttcag agcattgaat agccgctatg atcaaggttc tgctcgtcga
     3661 agataatgac gtcgatgccc ggctgacgca agatctcctt gcggaatgga gcgtcgaaga
     3721 atttcaagtc gtccacgtga tcaccctcgc ggagggatta gcccgcctga ctggggaacg
     3781 attcgacgcc gtactattag atctttcgct ccccgacgcg ttcggcctcc caacggtgcg
     3841 acaagttcat gccacaagcc cctcgacccc agttgtggta ctcagtggtg ttagtgacca
     3901 aagcctggct cttcaggccg tccaacaggg ggcacaggac tatctggtga aggggcaagg
     3961 gcaccccgaa ctgttggctc gcgctgttcg atatgccatt gagcggaagc gtacggaaga
     4021 acggctgatc tatctcgcac agtatgacca tctgactgga ctggtgaatc gaacattgtt
     4081 tcgcgaccga ctgtttcagg gcatggcgcg cagcaagcgc ctccagcagt cactgggctt
     4141 gatgttactt gacctcgacc gctttaagat ggtgaacgat acgatgggac atgatattgg
     4201 agatgagctt ctcaaatctg tggcagaccg gctcaagtgt tgcgttcgag aagtcgatac
     4261 ggttgcccgg atgggaggag acgagtttac gattattctg gaaggggttg catcagtgga
     4321 gaatgttacg gtagtggcga aacgaattgt cgaagctatt gcggcctcgt tcgagatcaa
     4381 gaatcgtcag atctctatcg gtgtcagcat cggaattacc atttatcccc acgatgataa
     4441 agagattgac gacttgctta aacatgcgga tgacgcgatg taccaagcta aacaggaggg
     4501 cggaagtgga ttccgattct atggatccaa cgaaccgcag acacccgacc tctactctca
     4561 gttcagtcag accccgcgaa cgtagcgcaa ttattttctc tctgcctttt gcacccctct
     4621 ccgtcacccg gccacgcctt tccttcccgg aacggactat cgaccgatat cagccaacgg
     4681 cttctccgtc acacagactc gcccccgagg gttcggcatg aagacattcg attgttcaaa
     4741 atctgcggcg tcaggcgtcg gatgacaatc cggtgtgagg acgatccccc actggttatt
     4801 gtcagcgcac gtgttatcaa tgagcagcgc ctcagcatga tgaaacagca atatcccgct
     4861 gagcaagttt tcgcgacagt ggttccgaac aagttcaggg tgacttccag gatcgcgaac
     4921 ggcgatgccg aagtgatgat ttccaaaaca tcgattgtcg gcgagcctgg gctgggctcc
     4981 ggcaaacaca aagactcccg attcacggtt gtggcacacc tcgttatcga tgaatgccgt
     5041 gcgcgactgc ggaccataaa tgacaacgcc agagagaatg ccgtcggtgg cgcgacactg
     5101 cgtgacagta caagaagaat ccatgaggtt catcgcggaa ttctgatcgc tcccaacata
     5161 ccgaaatgta attccgctga tccgtccacc gggcacccgt tggagataca gcggcccacc
     5221 ccgacgtgag aagatctgaa cctgatctcg tcctgccccg atcattagga tcggccgacc
     5281 cgagacgaaa atcttgtctt cataagttcc cggtcgaata aagacttggt cggtctctcc
     5341 cgcatcgttc aacgcagcac taggccgtgc atatgcgctg acatcctgcg catcgacgac
     5401 gagggtctta ccgccgagtg ggttggttgg tggatcggtc ttcggtatcg gtttctgctc
     5461 cactaacatc gcctcacttt gcgcccggat cgttgggggc gaggagacaa gaaaagacac
     5521 ggcattattt cggtcgttta aggggaagtc aagattgggg aaaatccatg gccagcgcca
     5581 tgttagcgga acaccaatct cgaatttgtc aagcgggcag agagccccaa cccgtacaaa
     5641 tctgtctggt cagcatgtga tgctatccaa atttgcagcc agatgttcgc atcgaagctc
     5701 ctggttttcc atcgattttc accggacgga tcctctccgc tgtggcacgg tgattgcgac
     5761 actacgtggc gatgcgatgc agaactctcg ggtcatggag gcgatcatga tgaacagact
     5821 gaagcatccg ttgctcgtaa ctgctgccgt ccttttctcg ttcaatcttg ctgtcgctgc
     5881 atcacctcaa ttgcaggaca cctcccgaca tctgtatgac cgagtgatgg aggagtttaa
     5941 acacaaagat tatgaagcgg cgctcgccgg cttccgtttc ttcctcgagc tccacggtca
     6001 atcatcattg gccgcaaacg cgcagtattg gatcggagaa tgccactacc ggttgggtca
     6061 ctacaaagat gcgctaaagt ccttttacga tgtcgtgtcc tattatccgc tcagcccgaa
     6121 gttggcggcc tcatccttga agatcgccca aacctacacg aagatcaaag accacgagaa
     6181 agcccgcatg atgtatgaac gtgtggttga tcagtatcct gacagtgcag aagccgagat
     6241 tgccagaaaa gcgttagagg cggcggccgc acgggatgag ccgacgacgt tgcccactga
     6301 atagctcagg agccatgtct gcgccgatga caagtaagct aatcggagtc gccgagacca
     6361 agcagcgtgg cgagttgcgg aatcgtatag gaggtatgcg cgggcaaggt cgtgaatcga
     6421 atgcccgcag actcgacgac agccttaacg atctcctgcc ggtctccctg gcgggagtga
     6481 gacggttgat catcctctaa ctgcactacc tgctccacga gtctcgaacc gggatggacc
     6541 agcacaaaat cgagtcgttt gagtgcaatc cgatgcagca agtctgaacg gatagctcct
     6601 tccgcctcca catgaacaat ggaccagagc ggaacctgcg tgaatacaag gtactgatct
     6661 tgaaccgcca gccgcatgag gttatagaga aacagttccg aatccgtgag cagcggctgt
     6721 ggctggagcg tcacatgggg aggcatcaca aaggtccccg ttttggcctt gatggaaggg
     6781 gacgggacgg caaaccacca caccagcaaa gcgactccga ttccaagacc gatcagtccc
     6841 caatccatcg cgccctttat cggctatgca gctgctcacg ccactgagga ctaccgggca
     6901 cgatcgttcc caataacacc cgatgcgaag caccagtcac ggcagggata ttcccccact
     6961 gtccagtggc ggtttgataa gccaggatgg caaacgcgac cgcttcaaag gctttactat
     7021 cccatccaag cgcctcaaag gtcgtcacgc ggaccgggtc aaacacggtg gcgagatgag
     7081 ccatgatcgc ccgattgcga acaccgccgc cgcccaccac aacttcatca attttgccat
     7141 gaatccatcg ccgtgcagtt ccaactgctt tagccgtcca catactgcag gtggctaata
     7201 gatcttctac agaaagttcc agctgttgtt gcgcggtaat cagatcctcc atcaaatgcg
     7261 gaccgaattc ttcacggcca gtcgatttag gcggccgctt cgacagaaag ggatgcgcaa
     7321 gcagtttgcc cagcagtcgg tcattcaccc gccccttcga cgcgagtttt ccttcacgat
     7381 ccattaagag acgtccggcg gtgagcttca cgatcagact atcgaggacc atgttcgccg
     7441 gcccagtgtc aaatgcttga acacctccct gtccgccgcc cggaggcacg taagtgacgt
     7501 tgctgattcc acccaagttg acaatcagtc ggccacgacg aggatgcttg agtaaaaggg
     7561 catgtgccat tggtgcgagg ggagcccctg gtcctccggc agcaatatca cgtggacgaa
     7621 agttcgcgat tgtcgtgatc ccggttcgct cggcaatgac tgcc
//
LOCUS       DATDNW010000120         6395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1309338, whole genome shotgun sequence.
ACCESSION   DATDNW010000120 DATDNW010000000
VERSION     DATDNW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309338"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..604
                     /locus_tag="VI359_03035"
     CDS             116..604
                     /locus_tag="VI359_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY6973259.1"
                     /translation="MATFPSIRTLLRQILHLRETPQRTAVAFAIGVWIAFCPFYGFHT
                     ILVGLCTAIFGLNFLALFAGASINNPWTIVPVLGATYWTGAVLLGRSETPVFDWHDLS
                     FSAIYEQVIPYAIPFAVGGFVLSFVGAALSYPVAHYLISKYRRTHPIPSAEPLPPHDP
                     LG"
     gene            625..2046
                     /locus_tag="VI359_03040"
     CDS             625..2046
                     /locus_tag="VI359_03040"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY6973260.1"
                     /translation="MQPSDHPSNAPYDGSALIADPIHQYVSFTVPYANPDPHERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHSRFVHSLGTMHVAGRFARHLYPFLKKAAKD
                     APSANYVEEFLRITALVHDIGHGPFCHFFDDNYLHALHSSHEKLGQIIIREHLGPAIR
                     KIRRSPSGSFDKGEELNPDQIAHVILKEKSKDNSRIPRWLNMLQPVISGSYTADNLDY
                     VLRDSYMCGVAVGPVDLSRLIHYTIITDKGFTIHKTGLPALQMFLNTRMYLYSNVYFH
                     RTTRAIDIHLRDIFGDTMKLLFPHDPRKKMDEYLTLTDWSLLQQVRNWKTSRQAAERR
                     LGEEWSRIIGRDIKWKMAYSTVLKEKGQERGMDFPSHEHFEKQIRKELPAPLQKIAFR
                     VDMAPLDPRPDPKDRRGNPLFVYDPGTKGVSTDPLEEFLDLLPTRLIQFRIYTLGHEQ
                     DAALSRAAATVLNKTPSSLETNF"
     gene            2201..2569
                     /locus_tag="VI359_03045"
     CDS             2201..2569
                     /locus_tag="VI359_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973261.1"
                     /translation="MKTWTMLILLALWFSGQTAVLAQTPIGAQKQADILKLMSELPPD
                     VMMKIQQLGKILQQDLKDGKLTQEQIQEELVSGNLEQKLRDLNPEAGPLLDDIAQAMK
                     NHPNAENLPHILDGLAGPAK"
     gene            complement(2587..2754)
                     /locus_tag="VI359_03050"
     CDS             complement(2587..2754)
                     /locus_tag="VI359_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973262.1"
                     /translation="MERGGAVNPGYRLIIVDQAGVLVSEFQLTEDALAQPDTFVAAIK
                     QAIEDVEEEEL"
     gene            2865..3440
                     /locus_tag="VI359_03055"
     CDS             2865..3440
                     /locus_tag="VI359_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6973263.1"
                     /translation="MAMASVMLPLGTPAPPFTLRDVVNGHSYSLDAFSGKTALLVMFI
                     CRHCPYVQHVERALAKLGQDYADTGLGIIGISSNDPVHYPDDAPERLKEMAHRLSLTF
                     PLCFDETQEVAKAYKAACTPDFYLFDRERKLVYRGQLDDSRPGNNKPVTGRDLRTAID
                     AILASKPVDPNQKASIGCSIKWKPGNAPAYA"
     gene            complement(3572..4813)
                     /locus_tag="VI359_03060"
     CDS             complement(3572..4813)
                     /locus_tag="VI359_03060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6973264.1"
                     /translation="MHTSTHTSFHTLGLSEALLQSLTKAGFLQPTAIQAQAIPHALAG
                     RDVLGCAQTGTGKTAAFVIPLIERLTAKPKGHPRGLILVPTRELAIQIQDTIDRLGQQ
                     MRIFATTIVGGADMPAQVRGLRQHPDILVATPGRLLDHMWNGTVNLGVMEILVLDEAD
                     RMLDMGFAPQINQILDALPEERQSLLFSATLPTDLRRLAQASVKNPVHVMVARPATTA
                     AGVTQALHHTTHDRKTDLLVSLLRNESDTVLVFTRTKHRADRLGRALGSAGHKVAVLH
                     GDRSLPQRRAALDGFKRGTFRVLVATDIAARGIDVANIGHVVNYDLPHCPEDYVHRIG
                     RTARMHATGRATSFVTAEDHTQLRAIEQLLGQAVPLAVGSQAPHVAHSSDSLRSSHHR
                     QGRHGRPSGFRTRSFATQTPS"
     gene            complement(5131..5868)
                     /locus_tag="VI359_03065"
     CDS             complement(5131..5868)
                     /locus_tag="VI359_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY6973265.1"
                     /translation="MPLMPVIVFVTVLLALPIAAQAHDETKPEIPTLTVVATGSVTAP
                     PDRAFVTVGMDSAGKSLAEAQRQNSAVMQKVIERLKELKIEKERIQTSAFTVSPQYKS
                     SSRRSSDAPPAAPEIVGYTVSNMLTVEVRETEKVAPVIEEALSAGANHFQGLQWALRD
                     EQQARLNALKLASAKAREKAAALSEALSVKLGRLVNVTEGSQVLHPTPRVGRAMAAME
                     GAGGEVPIASGEMRVEATVTLVYEIAH"
BASE COUNT         1330 a         2058 c         1751 g         1256 t
ORIGIN      
        1 gggacgattc gaccggccag gctgggcatg cggacgcggt cctccaagac agggtcccgc
       61 cccttgccgg tcaccagtta cgaacacttc cgataaccct gttcacagag agaccgtggc
      121 cacctttcca tcaatccgca ccctgcttcg gcagattctc catcttcgcg aaacaccgca
      181 gcggactgcg gtggccttcg ccatcggagt ctggattgcc ttctgcccat tttacggatt
      241 ccacacgatt ctcgtcggcc tgtgtacggc gatcttcggc ctcaactttc tggccctctt
      301 tgccggcgca tccatcaaca atccctggac catcgtcccg gtgctcggcg cgacgtactg
      361 gaccggtgct gtgctcctgg gccggtccga aaccccggta ttcgattggc acgacctgag
      421 cttctccgcc atctacgagc aggtcatccc ctatgcgatt cccttcgccg tcggtgggtt
      481 cgtcctcagc ttcgtcggcg cagccctctc ctatcccgtc gcccattatc tcatcagcaa
      541 gtatcgtcgc acgcatccca ttccttcggc agagccattg ccgcctcatg atccgctggg
      601 ctaagatgga gatgcggcat tggtatgcag ccatccgatc atccatccaa cgcgccctat
      661 gacggctcgg cgctcatcgc cgatccaatt caccagtacg tctcattcac cgtgccctac
      721 gccaatccgg atccgcacga acggacggaa aaggatctga tcgactcgcc ctgggtacag
      781 cgacttcgct acatctatca actccaaagc gcccggtggg tctatccctc ggctgaacac
      841 agtcgcttcg tccattccct cggcaccatg cacgtggcag gccggttcgc gcggcacctc
      901 tatccgttcc tcaaaaaagc ggcgaaggac gccccttccg ccaactatgt cgaagagttc
      961 ctccgcatca ccgccctcgt gcatgacatc ggccatggcc ccttctgtca ctttttcgac
     1021 gacaactatt tgcacgcact gcactccagc cacgaaaagc tcgggcagat catcatccgc
     1081 gagcatctcg gaccggcgat tcgcaagatc cgacggagcc cgtccggctc cttcgacaag
     1141 ggcgaggaac taaatccgga ccagatcgcg catgtaatcc tgaaggaaaa aagcaaggac
     1201 aactcgcgca tcccgcgctg gctgaacatg ctccagccgg tgatttccgg gagctacacc
     1261 gcggacaatc tcgactacgt gctgcgcgat tcgtacatgt gcggcgtcgc cgtcggtccg
     1321 gtcgatctca gccgcctcat tcactacacg atcatcacag acaagggctt cacgattcat
     1381 aagaccggcc tccccgcgct gcaaatgttt ctcaacaccc ggatgtatct ctattcgaac
     1441 gtctattttc accggacgac gagagccatc gacatccatc tgcgcgatat cttcggcgac
     1501 acgatgaagc tgctcttccc acacgatccg cgcaagaaga tggacgaata cctcacgctg
     1561 accgactggt cgctcttgca acaggtgcgg aactggaaga cctcacgcca ggcggcggaa
     1621 cggcggttgg gcgaagaatg gtcgcgcatc atcggccgcg atatcaaatg gaagatggcc
     1681 tacagcacgg tgctcaagga aaagggacag gagcggggga tggatttccc cagccacgag
     1741 cattttgaaa aacagatcag gaaggaactg ccggctccgt tgcagaagat cgccttccgt
     1801 gtcgacatgg ccccgctcga tccgcgaccg gatccgaaag atcgccgggg caatcccctc
     1861 ttcgtctacg acccagggac caaaggagtc tcgaccgatc cgctcgagga gttcctggat
     1921 ctcctcccaa ccaggctcat ccagttccgc atctataccc tcggtcacga acaggacgcc
     1981 gctctctccc gcgccgccgc caccgtcctc aacaaaacac cctcgagcct cgagacgaac
     2041 ttctaatatg gcggcaggct gtgccgggac gcgccgctgc cgtgcctcga attctttctc
     2101 tcgcaaattg agaaacagct gacaaccgga accgtcgttg agatactggc gggagccgtg
     2161 tgcatcacgc gatcccaccg cacggtcatg ggagggaatc atgaaaacat ggacgatgct
     2221 catcctgctt gcactctggt tctccggcca aaccgctgtg ctggcgcaaa cgccgatcgg
     2281 cgcccagaaa caggccgata tcctcaagct gatgagcgaa ctgccgcctg acgtcatgat
     2341 gaaaattcag cagctcggaa agattttgca acaggacctg aaagacggga aactgaccca
     2401 ggaacagatc caggaagaac tggtgtcggg caatctcgaa cagaagttac gagacctcaa
     2461 ccccgaagcc ggtccgctgc tcgatgacat cgcccaggca atgaaaaatc acccgaacgc
     2521 cgagaacctg ccccacatac tcgacggact ggcgggacct gccaagtaac cgccgcaagg
     2581 ctcatttcaa agctcttcct cttcgacgtc ctcgatcgcc tgcttgatcg cagcgacgaa
     2641 cgtgtccggt tgagccaacg cgtcctcagt cagctggaat tccgaaacga ggacgcccgc
     2701 ctggtcgaca ataatgagac ggtagcctgg attcacagca cctccccttt ccatgcaaca
     2761 tgcagatgcg aggtcagacc agtgatgcag actggctcta gtcgtcgaga gtccggacct
     2821 gtagaatgga tcgaccgttt ccaccatgac accacgaggt tcgtatggca atggcgtcag
     2881 tgatgctccc gctcggcacg ccagctcctc cctttacatt gcgcgatgtc gtgaacgggc
     2941 atagctactc cctcgatgcg ttttccggca agaccgcttt gctggtcatg ttcatctgcc
     3001 ggcactgtcc ctatgtccag cacgtcgagc gagcgctggc caagctggga caggattatg
     3061 cggacacagg tctggggatc atcggcatca gcagcaacga tccggtgcat tatccggacg
     3121 acgcccctga acggctcaag gagatggccc accgtctcag cctgacgttt ccgttgtgct
     3181 tcgacgagac tcaagaagtc gcgaaagcct acaaggccgc ctgcacgccg gacttctatc
     3241 tcttcgatcg agagcggaag ttggtctacc gagggcagct cgacgacagt cgtcccggca
     3301 acaacaagcc ggtcacaggc cgcgatctcc gcaccgcgat cgacgcgata ctcgccagca
     3361 agccggtcga cccgaaccaa aaagccagca tcggctgcag catcaagtgg aaacccggca
     3421 acgcgccggc ctatgcctag tcgagcagca gcaacacgcg aacccaccta cgattcgatc
     3481 tgtaagggta gccggggcag gatggcggac cgacgcgacg cgcgcgacgc cactgagatg
     3541 aattggcacc tcaatggcgt ccggaaggag gttatgacgg ggtctgcgtg gcgaagcttc
     3601 tcgttctgaa tcccgacggt ctcccatgtc gtccttgtcg gtgatgggat gacctcagcg
     3661 agtcagagct gtgcgcgaca tggggagcct ggctgcccac tgcgagcggg actgcctgac
     3721 caagcaactg ttcgatcgcg cgcagttgcg tgtggtcctc ggcggtgaca aagctggtcg
     3781 cccgaccagt cgcgtgcatg cgggcggttc gcccgatccg gtgcacgtag tcttccgggc
     3841 aatgcggcaa gtcgtagttg accacatggc cgatgttggc cacatcgatc ccacgggctg
     3901 cgatgtccgt tgcgaccaat acccgaaacg tcccgcgctt aaatccatcc aacgccgccc
     3961 gtctttgcgg gagactccgg tcaccgtgca acacggcgac cttgtgtccg gcgctcccca
     4021 gagcgcggcc cagccgatcg gcccgatgct tcgtccgggt aaagaccagc accgtgtccg
     4081 attcgttccg caacagcgag accaggagat cggtctttcg atcgtgggtg gtatggtgca
     4141 acgcttgtgt gacgccggcg gccgtcgtgg ccgggcgtgc caccatgaca tgaaccggat
     4201 tcttcacgct cgcctgagcc aggcgcctga gatcggtcgg caaggtggcg gaaaagagca
     4261 gtgattgccg ctcctcgggc agcgcgtcca ggatctgatt gatctgcgga gcaaagccca
     4321 tatcgagcat gcggtccgcc tcatccagaa cgaggatctc catcactcct aaattcaccg
     4381 tcccgttcca catatgatcc agcaggcgac caggcgtggc gacgagaata tcggggtgct
     4441 gacgaagtcc tctgacctgg gcgggcatgt cagcccctcc gacgatcgtt gttgcaaaga
     4501 tccgcatctg ctggccgagc cggtcgatcg tgtcttgaat ttggatcgcc agttccctgg
     4561 tcggcaccag aatcaggccg cgcgggtgac ctttgggctt tgcggttaac cgctcgatca
     4621 gcggaatcac aaaggcggcc gtcttaccgg tcccggtctg cgcacagccg agcacatcgc
     4681 gtccggcgag tgcgtgagga atggcctgcg cctgaatggc ggtaggttga agaaaacccg
     4741 ccttggtcag gctctgtaaa agggcctcgg acaaaccaag ggtgtgaaaa ctcgtgtgag
     4801 tactcgtatg cactaaacta tcctttcagt ttgatcgcat gttcacgaag atcagagaac
     4861 cgaggggagg cagattcagg aagggagaga gtccgctccg aacaggacct ggtcgcgatg
     4921 cgatcgcgag gtccgtaatt gattcaacat cgccatcacc ggaccactac aatgacgata
     4981 tggcgtcacc ttacagcatg cagtcccggc cgtcaaccta tttaataaga cgtcaccgtt
     5041 cacacgaacg gagatcccgg gcacagacac cgtccaatgt cacgccggct cgcgtcagcc
     5101 tgcactggtg gccggtttca gcaccatgac ctagtgcgcg atctcataga ccagggtgac
     5161 ggtcgcctcg accctcatct cccctgaggc aatggggacc tcaccgcccg ccccttccat
     5221 ggccgccatg gcgcgaccga cgcgaggcgt cgggtgaagg acctggctgc cttccgtcac
     5281 gttcacaagt cggccgagtt tcacgctcag tgcctcactc agagccgccg ctttttctcg
     5341 cgccttggcc gaggcgagct tcaatgcgtt gagccgcgcc tgttgctcgt cccgcagggc
     5401 ccattgcaac ccctgaaagt ggttggctcc ggctgacaac gcctcttcga tcacgggagc
     5461 caccttctcg gtctcgcgga cttcgaccgt cagcatatta ctgactgtgt acccgacgat
     5521 ttccggcgca gccggcggag catcggagga acgcctagat gaagacttgt attgcggcga
     5581 caccgtaaac gcagaggtct gaatgcgctc cttttcgatc ttcaactcct tgagacgttc
     5641 gatcaccttc tgcatcacgg ccgagttctg gcgctgggct tccgccagcg atttgcccgc
     5701 tgaatccatc cccacggtca cgaatgcccg atcagggggc gcggttacgc tgccggtcgc
     5761 gaccaccgtc agcgtcggga tttccggctt cgtctcgtcg tgggcctgtg ccgcaatcgg
     5821 caatgcgagt agcacagtta cgaaaacgat cactggcatg agtggcatgg tcgttctctc
     5881 cacggctgaa aagcactcaa tgaaccgaga caccgtacgg tatcgccgaa ggctaggctg
     5941 tccgccaaga ggtgtcaaga agctaagtga gcaacgcggc ctggtggtcc tcttgctcgt
     6001 ggatcgccag acctcagaca gaccattccc ccattccccc catcccgccc gtcccgcttt
     6061 tctcgcccat cgcgacccct cccaatcacc acagaacccc aaaagactcc caacccctgc
     6121 ggttcgacgc aacttgccga cgatctgttc tttcgtgtat tttcatcgac gcatgatgtc
     6181 ctccttctca agcttcgctc ctcgcggatt cctcccgtac aacctggttc tgctttaaac
     6241 cgaacagggc ataccgactg atgctacgaa tcgtgaagat aattgcgagg agtcttatgg
     6301 ttacgatgac agcatgcgca ggcatcgctg tggcggaacc ctttgtcgct cataccgatc
     6361 tagagctgcg agttggtgaa gaatggctgt gcggg
//
LOCUS       DATDNW010000121         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_153529, whole genome shotgun sequence.
ACCESSION   DATDNW010000121 DATDNW010000000
VERSION     DATDNW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153529"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            729..2363
                     /locus_tag="VI359_03070"
     CDS             729..2363
                     /locus_tag="VI359_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6973266.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGNVRVMGFPPIGVMSLSSVL
                     KQAGHDCVMFDQANPDTPNDVIIEMIKRREPALVGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLDLLAHLNDPEGVASLTWTR
                     DGRVVNNPGRPMERHLDQWPFPDRESLKLDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVDELKYLEEHGYGAVYFVDDHFLLQPKRIEAICH
                     GVTSAGLSIQWGIEGRVDSVAQHLFPAMAQAHCRTVMFGIESGSQKILDRLKKEQTLA
                     EVTTAVTNAKKAGIEIVHGFFTVGNPDETVEDMRATFNFASKLPLDTFGFNRLCVYRG
                     TPLWQEYVKRGLVSDATDWYKYFKCSEIDPTCLPGEVINDVRQQGLKKLFLYKLMHYP
                     LQTYRLLRRFLRFMPARDVIYLIMKPFLGKRHGATKAEVLSRAVEHAEMKDAAAQLTQ
                     VSEEILHAVLQSSRVERLRIQLEAEGLRELPMVQSR"
     gene            complement(2373..>2985)
                     /locus_tag="VI359_03075"
     CDS             complement(2373..>2985)
                     /locus_tag="VI359_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY6973267.1"
                     /translation="EALRGILMDEFKHEDVLVTELTKRKINPERIRNIFLGMNDGLVE
                     ILGAVSGFFAAFGSATMVLIAALTTAVAGALSMAAGSFLALNSEKEVKTTEIARKVFL
                     GEESDVTPMEESPLGSAVVVGVAYITGALVPVLPVLVGAKDALFSVFTAGLMVIVVST
                     ILSFLSGMDVKRRIILNLVIITVAVGVTYAIGLAAKEIWGISV"
BASE COUNT          655 a          842 c          831 g          657 t
ORIGIN      
        1 cacgagtcag tttctttctg aacaagttca ggaagctggg cttcatcgat tataacggcg
       61 gattacgcat tcgcagttcc ctcctcaaca tcgtcctggt cgactaactt cccatgtgtc
      121 gcgtagggtc ttaggctcaa ggcgtagcct cgcctgccgg ccgcatccat tcctcaaaag
      181 tgagcaattt tggccagttc gccattctgt gtctctggca taatttcact caaattcaga
      241 cgatgcgcat cgagattctc cggcaccttc ttacggtaag ctccgccgaa cgttggagaa
      301 tcggttgtcc tgatgcgagg atgaaaattc aaaagagggc agtgttgatg ggataccgct
      361 tgctcgcggt gaatgatgac cgggctgcgg accgcctgaa cgagctacga tcatgctgct
      421 tcagaaccgg agggccggtg ctgcgcccca gcgatgtttt ttctttcctc gcatgccgaa
      481 tcgtgctaac ttcttctcac cagttaggat cacaccttct acgggatctg cgcagaagcc
      541 ccctcggcag attagaaagc aggaagcact ctctcctcgg aatagacagt taccccgtcg
      601 ttttggttcc cttgaaagaa ccccgaacca ggacgcgaaa gttgcagtcc ggcattgtct
      661 tagccggaag cagtaaggcc ggctcgcggt aagacggcgt gtgaaccatt ccgcgcttgg
      721 aggtaggtat gctcgtcttt ctgatccacg tccgggatcc gcaattctat gcccttccgg
      781 ctaaaacccg cgccaagaac ggcaatgtcc gggtgatggg gttcccgcca atcggcgtca
      841 tgtcgctgtc gtccgtgctc aaacaggcag gacacgactg cgtcatgttc gaccaagcga
      901 accccgacac gccgaacgac gtgatcatcg aaatgattaa gcggcgggag ccggcgctgg
      961 tcggcctgag ctttctcagc accacaagct atccctacgc gaagatcctt gcccgtcaaa
     1021 ttcgcgcagc cgattcaacc gtgaaattag cctttggcgg cgtgtttgcc agtctgaatg
     1081 ccggactggt gaagctacaa tgtccagaag tcgattttgt ctgccgtggg gatggggaac
     1141 aactgctgct cgatcttctg gcgcatctga acgatcccga gggagtggcc agcctgacat
     1201 ggacgagaga cggtcgtgtg gtgaacaacc cgggtcgtcc gatggagcgg catctggatc
     1261 aatggccgtt tcccgatcgt gagagcctta agctcgactt tgttgagtcg atgccgttgg
     1321 atgtgccggc ggtgttgtcg atggaacggt ttacaaccat gcaaacttcg cgcgggtgtc
     1381 cctggccttg cgtgttctgc gatatcccca tttttaacga gggcaagtgg cgcgcacgga
     1441 gcgctcagca cgtggtggat gagctgaagt accttgaaga gcatggctat ggagccgtct
     1501 acttcgtcga tgaccatttc ctgcttcagc cgaagcgaat cgaagccatc tgtcatggag
     1561 tcaccagcgc cggtttgagc atccaatggg gcatcgaggg tcgggtggat tcagtggctc
     1621 agcatttgtt tcccgccatg gcacaggcgc actgtcgcac cgtcatgttc gggattgaaa
     1681 gcggcagtca gaagattctc gatcggctca agaaagagca gacgttggcc gaggtgacta
     1741 cggctgtcac caatgccaag aaagccggca tcgagatcgt gcacggcttc ttcacggtcg
     1801 ggaatcccga tgaaacggtc gaggatatgc gggcgacgtt caactttgcg tcgaaactgc
     1861 cgctcgatac cttcggtttc aaccggctct gcgtctatcg aggaacgccg ctctggcagg
     1921 aatatgtcaa gcgggggttg gtcagcgacg ccaccgactg gtacaagtac ttcaagtgct
     1981 cagaaatcga tccgacgtgc ttgccgggag aagtcattaa cgatgtccgc cagcaagggt
     2041 taaagaaact cttcctctac aagctcatgc attaccctct gcagacctac cgtttgttgc
     2101 gccggttctt acgttttatg ccggcgcgcg acgtaatcta tctgatcatg aaaccatttt
     2161 tgggcaagag acatggggcc acgaaggccg aggtgctctc acgagcggtc gagcatgcgg
     2221 agatgaaaga cgcggcggca cagctcacgc aggtgagtga ggagatattg cacgcggtgt
     2281 tacagtcgtc gcgagtggaa cgccttcgga ttcaactaga ggccgaaggg ttgcgagaat
     2341 tgccaatggt gcagagccgc tagcagccag gcttacaccg aaattcccca gatttcttta
     2401 gcggccaacc cgattgcgta ggtcacgccc acggccacgg taataatcac cagattgagg
     2461 atgatgcggc gtttcacgtc catgccggac aaaaacgata agatcgtcga gaccacaatt
     2521 accatgagcc cggcggtgaa gaccgagaac agcgcatcct tcgcgccgac gaggaccggc
     2581 aacaccggca ccagtgcgcc ggtgatatag gccaccccca cgaccacggc cgatcccagc
     2641 ggagattctt ccatcggtgt gacatccgat tcttcgccca agaacacttt tctggcgatt
     2701 tcagtcgtct tcacttcttt ttcgctgttc agggccaaga acgaaccagc ggccatcgac
     2761 aacgcgccgg ccacggccgt tgtcaaggcg gcaatcaaga ccattgtcgc actgccgaat
     2821 gcggcgaaga agccgctcac cgctccgaga atctccacca acccatcgtt cattcccaga
     2881 aagatatttc ggatcctttc cgggttgatc ttccgcttgg tcaattccgt cacgagcaca
     2941 tcctcatgtt tgaactcgtc catcagaata ccccgcagcg cctcg
//
LOCUS       DATDNW010000122         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1104810, whole genome shotgun sequence.
ACCESSION   DATDNW010000122 DATDNW010000000
VERSION     DATDNW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104810"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VI359_03080"
     CDS             <1..1107
                     /locus_tag="VI359_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973268.1"
                     /translation="GQRLVLDGSAAGWAVRHRKPRVDHDLASTQGRFLDHKHLFKDRF
                     QSSLVVPFFVRGQVGGTITLSSKEAGRYQATDARTLEPIILKLADALQAPAPTPPGPT
                     PALGSVDTPEGAVAPTSLEPIIRKQERHAAIGEFSAFLATEVREPLGSIRAQLEELTG
                     EGILDFDPQTRVENAMRELMRVEAILNEILDFAKPLELNRRLCRIPEVLESALTVVGT
                     DLEVTRIQVTKDYATQIAPVRGDESKLQQVFLSIFKNAVEAMSPGGHLHIQVTQHRAG
                     RGLEDQILIKNDGAPIPAEIVDKVFEPFFTTKRAGTGLGLATVKKIIEEHGGSIAIGS
                     APGEGTTVTIRLPGISRGPAFRHRGRGRRPLHRR"
     gene            1273..1641
                     /locus_tag="VI359_03085"
     CDS             1273..1641
                     /locus_tag="VI359_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973269.1"
                     /translation="MKLVIGTVATLSGVLFLLSMASANPALLPKHEGYPMKNSGSPVN
                     GQATANDPGQTNASGEKAELKAAAFDDTHVKQNLMKTDNARITQGQGANQLPTVQGPQ
                     IKIAPPVTSATKADASLGVK"
     gene            complement(1719..2324)
                     /locus_tag="VI359_03090"
     CDS             complement(1719..2324)
                     /locus_tag="VI359_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY6973270.1"
                     /translation="MLILFFCAELAPVRNVPHQILSRAYFNRPTVQVARSLIGKFLVR
                     SCNGRELAGKIIEVEAYVGPQDKACHASKGRTARTDVLFGPPGISYVYLIYGMYHCLN
                     VVTERTEFPAAVLIRAIEIEGELIDGPGRLCRAMQIDRSLNRWNMTDGNQLWFEDRSV
                     AVRKSDVGAFTRIGVDYAGKWAKKPWRFRLNGVESESRKRS"
     gene            complement(2486..>3428)
                     /locus_tag="VI359_03095"
     CDS             complement(2486..>3428)
                     /locus_tag="VI359_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6973271.1"
                     /translation="KRVPTGTIVESAVQEASYSPYMKLEVQPMPWMRLSGGLRADYFT
                     FDVRNLCDTCPQQPDGRINSGQVSPKANLILGPWFNTELFVNYGTGFHSNDARSTVTG
                     GSSPLARAQGTEVGIRSKPWGPERMELFSTFWWLDLKSELVFVGDAGTTEARGPTRRY
                     GVEVGARGQVYGPLYFNGSFTWTHAEFKDTGLAIPLAPELTAYAALLLRWPEGLSSQI
                     QMTYLGVRPLTEDRTINAPSWLDFDLTERYLLPVKLPHGHLEAIFYVQNLFDTKWEQA
                     TFAFTSRLPGEPAAGAQDIHFVPGNPRFVMGGLAWYF"
BASE COUNT          773 a         1034 c          919 g          702 t
ORIGIN      
        1 ggtcagcggc tggtgttaga tggctcggct gccggatggg cggtgcgcca ccgcaaacct
       61 cgtgtcgatc acgatctggc ctcgacccaa ggacggtttc tcgaccataa gcacctcttc
      121 aaggatcggt ttcagtcctc gctcgtcgta ccgttcttcg tccgcggcca ggtgggcggc
      181 accattaccc tctcgtcgaa agaagccggt cggtaccaag caacggacgc gcgcacgctc
      241 gaaccgatca ttctcaagct cgccgacgct ctgcaggctc cggctcctac gccacccggc
      301 ccgacaccgg ctctgggatc ggtagacact cccgaaggcg ccgtggcacc tacctcgttg
      361 gagcccataa tccgcaagca agaacgccat gcggcgatcg gtgaattcag tgcgtttctc
      421 gcaacggagg ttcgagaacc gctgggatcc atccgcgcgc aactggagga attgacggga
      481 gagggcattc tcgattttga tccccagacc cgtgtcgaga acgccatgcg cgaactcatg
      541 agagtggaag caatcctgaa tgaaattctg gacttcgcaa aaccgctcga gctgaatcgc
      601 cgtctctgtc gaattcccga ggttctcgag agcgcactga ctgtcgtcgg aaccgattta
      661 gaagtcacgc ggattcaagt gacaaaagac tatgcgacgc aaatcgcccc agtgcgcggc
      721 gatgaatcca agcttcaaca ggttttcttg agcatcttca agaacgccgt cgaagccatg
      781 agcccggggg ggcacttgca cattcaagtg acccaacatc gagccggtcg aggtctcgag
      841 gaccaaattc taatcaagaa cgatggggca ccgatcccgg cggagatcgt cgataaggtc
      901 ttcgaaccgt tctttacgac gaaacgagca ggtaccggtc tcggcctcgc gaccgtgaag
      961 aaaatcattg aagagcatgg gggttcgatc gccatcggca gtgcgcctgg cgaaggcacg
     1021 acagtaacga tccgccttcc cggtatcagc cgtggccccg ctttccgcca ccgtggtcgt
     1081 ggtcgccgtc ccctccaccg acgctaggat tttccacaaa ccctgctttt tctgtcgttt
     1141 atcacgcagc tcgtttctcg cttgacagag atagcccccc ctggctatca tcccgccact
     1201 tgattcgctt ttagaatgaa catattcatc aacgtattcc ttcagccacc tttattcaca
     1261 aaggagtagg gtatgaagct cgtaatcggc actgttgcga cactgtccgg tgtgctgttc
     1321 ctgctgtcga tggcgtccgc caatccagcg ctgctcccca aacatgaagg ctacccgatg
     1381 aagaacagcg gaagccccgt gaacgggcaa gcgactgcca atgacccggg ccagaccaat
     1441 gcgagtggtg agaaggccga actcaaggcc gccgcgttcg acgatacgca tgttaagcag
     1501 aatcttatga agacagacaa tgcgcggatt acccaaggcc agggcgcgaa tcagctccct
     1561 accgtgcagg gaccacagat taagatcgcc cctccggtga cctccgccac caaggcggat
     1621 gccagtctcg gcgtaaagta actcgaatcg tagcttcttc tgcgaggagg gagtcaggct
     1681 gcttgcctga ctccctcttc atgttttgac ggcatgtatc agctccgctt ccggctctcg
     1741 ctctccactc cattcagccg aaaccgccag ggcttcttgg cccatttccc cgcataatcg
     1801 acgccaatgc gtgtgaatgc accgacatcc gacttccgta ccgcaacaga ccgatcttcg
     1861 aaccacaact ggttcccatc ggtcatgttc caccggttca aacttcggtc aatctgcatc
     1921 gcccggcaca atcgcccggg accatcaatc aactctccct cgatttcaat cgcccgaatc
     1981 agcacggccg ccgggaactc agtccgttcg gtaacgacat tcaggcaatg atacatgccg
     2041 tagatgagat acacgtagga aatccccggc ggaccaaaca agacatcggt gcgggcggtt
     2101 cttcccttcg acgcatgaca ggctttatcc tgaggcccga cgtaggcttc aacctcgatg
     2161 atcttgccgg ccagttcccg tccattgcat gaacggacga gaaacttgcc aatgagcgac
     2221 cgtgcgactt gcacagtcgg gcgattgaaa tatgcacgtg aaagtatctg gtgtggcacg
     2281 tttctcactg gagcgagttc cgcgcaaaaa aacaggatga gcaagacgcg cgtctcctgc
     2341 tttcgcgctc ctctcccaat tgccaactgc tcaaaatgac gtccagcaag gccgcaagga
     2401 gcgaagaccc ctaggagtac tattcgtgta cgttgagggg tcagagcaac tgagaacgaa
     2461 gctggaggat attttcagca actggctaaa aataccatgc gagaccgccc ataacaaagc
     2521 gtggattccc cggcacgaaa tggatgtctt gggctccggc ggccggctca ccgggcaagc
     2581 gggaggtgaa ggcaaacgtg gcctgttccc atttggtatc gaagagattc tggacgtaga
     2641 aaatcgcctc caggtgtcca tgcggcaact tgaccggcag caggtatcgt tcagtcaaat
     2701 cgaagtcgag ccaggatggc gcatttatgg tgcgatcctc cgtcaagggc cggactccga
     2761 gataggtcat ttggatttgc gaagagagtc cctccggcca ccgcagcagc aaggccgcgt
     2821 acgcagttaa ttcgggtgcc aatggaatag ctaaccctgt gtctttgaac tcggcatggg
     2881 tccaggtgaa gctgccgtta aaatagagcg gcccatagac ctgtccgcgt gcacccacct
     2941 cgacaccata gcgtcgcgta gggccacgtg cttcggtcgt acctgcatcg cccacgaata
     3001 cgagctcgga tttcaaatcc aaccaccaga acgtcgagaa caattccatc cgttccggac
     3061 cccagggttt tgaacgaatg ccgacctctg tgccctgcgc ccttgctaga ggtgaactcc
     3121 ctcccgtgac cgtcgaccgt gcatcgttgc tgtggaagcc ggtcccgtag ttgacgaaca
     3181 attccgtgtt gaaccacggt ccgagaatca gattggcctt cggactgact tgaccagaat
     3241 ttatcctgcc gtccggctgc tggggacagg tgtcgcaaag attacgcaca tcgaatgtga
     3301 agtagtcggc gcgcaggccg cccgacaacc gcatccacgg catcggttgt acctccagct
     3361 tcatgtatgg agagtaagag gcttcttgta ccgcgctctc cacaatggtt ccagtcggaa
     3421 ctcgcttc
//
LOCUS       DATDNW010000123         5346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_153828, whole genome shotgun sequence.
ACCESSION   DATDNW010000123 DATDNW010000000
VERSION     DATDNW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153828"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..392)
                     /locus_tag="VI359_03100"
     CDS             complement(69..392)
                     /locus_tag="VI359_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6973272.1"
                     /translation="MPIYEYRCGQCEKQFDATQSVHARPEDTVCPFCQAQQATRLLSA
                     FASKVVGTHKPGFSEMKAYDMLNERKDKFAKLPPVFGKRTAPPPMDAVASGSPPIDQT
                     KPSES"
     gene            519..695
                     /locus_tag="VI359_03105"
     CDS             519..695
                     /locus_tag="VI359_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973273.1"
                     /translation="MSLLIRKYKGHNGMMQEERIDDPDRIERYLRLFEKDDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            787..990
                     /locus_tag="VI359_03110"
     CDS             787..990
                     /locus_tag="VI359_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973274.1"
                     /translation="MIYWIHIARGIDRVTLHKDRCSEVPSTHLSSDFWEGGWFDYPDK
                     ERALLAMEQAGTNEQRRCPLCKP"
     gene            1075..1944
                     /locus_tag="VI359_03115"
     CDS             1075..1944
                     /locus_tag="VI359_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6973275.1"
                     /translation="MPRLALLLTTLIWGATFPATKVVLEQIPPLSFLFLRFSLGALLV
                     VIGMLLSGFPVRRERSVLRASAIATVWLFLGYLLQTVGLSYTTASNSAFITALYVVFV
                     PLILRRFGWRVWVATSIAMGGLWLLVKPTIAINAGDLLTLGCAVAFAAHIACLERFTR
                     EVDAPSLFTWQMVAMAVILCPAMVVEEAPVESFAPTAVLLVGLAITGGLATGAFAVQM
                     WAQRLIPAQQVALVFASEPVYAAWLSWYFLGESLDLQGWIGSGLIVAAVVIGAFATES
                     HGAEKPVASLQTA"
     gene            1974..2441
                     /locus_tag="VI359_03120"
     CDS             1974..2441
                     /locus_tag="VI359_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973276.1"
                     /translation="MSQKFGNSRWVREGYLDNRVEGTVVGRIHFAVIGAVDVYLGGNF
                     KSDIAGQVIQFRNRRFEEEDVAGQVIGDMENPQIGTVNLISFDPHPNLVPHPYIEWFS
                     IRKNHYRIELEPADAWVMSESEVATIESESQRIRETLALRESERPSHEESDWV"
     gene            complement(2525..3499)
                     /locus_tag="VI359_03125"
     CDS             complement(2525..3499)
                     /locus_tag="VI359_03125"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY6973277.1"
                     /translation="MSDVRVLHPSKAQALWFTILRWVDSWAGLEYLKVDGPPRMDWLR
                     CIPIIAVHLMCLGVIWVGWSWTAIAVAAAFYFVRMFAITGWYHRYFSHRTFKTSRACQ
                     LAFAILGGSCAQRGPLWWASHHRHHHIASDMPDDVHSPRQGGFLWSHIGWFTSQTHFA
                     PRLKAIADFAKYPELRFLDRFDILVPTIAGFAMFGLGRLLETYAPSLGTNGPQMLIWG
                     FFISTVVLIHGTCTINSLSHVYGSQRYDTGDDSRNNFFLALITLGEGWHNNHHYYPAS
                     TSQGFYWWEVDITYYGLKLMEWLGLVWDIRDVPAYVRDGKTKQEAIAV"
     gene            3824..4849
                     /gene="hemE"
                     /locus_tag="VI359_03130"
     CDS             3824..4849
                     /gene="hemE"
                     /locus_tag="VI359_03130"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY6973278.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMPEYRSLRAKHSILDL
                     CKTPELAAQVTLQPIDRFPLDAAIIFADILLPLEAMGLSLEFADGEGPVIHNPITDRG
                     SVERLKVIDGDELSYVADAVSLTRQALSGRVPLIGFAGAPFTLASYAIEGGGSRNYVL
                     TKQLMYREPDVWHKLMDKLARVVTGYLRRQIRAGAQAIQLFDSWVGCLAPGDYEEYVL
                     PHVQVVFEGLKREGVPLIYFGTGTSAILRQMRRAGGDVIGVDWRIHLDEAWATVGHDV
                     AVQGNLDPLVLFAPLHEIERRVEDILRRAAGRPGHIFNLGHGILPNTPVEHVAAVVDM
                     VHKLSAR"
     gene            4915..>5346
                     /locus_tag="VI359_03135"
     CDS             4915..>5346
                     /locus_tag="VI359_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY6973279.1"
                     /translation="MPGSRKPIAVLLMAMGGPDSLENVEPFLRDVRGGRPTPSELVEE
                     IRERYRVTGGKSPAFGITKEIAKKLEERLNEPGGERYRVYVGLRHWHPFIHETYAELL
                     SDDPEQVIGLCMAPQQSSLSTGAYRKKVDEVRASLQSDCPVS"
BASE COUNT         1139 a         1388 c         1619 g         1200 t
ORIGIN      
        1 tcgcttgtcg attcggacat cacggcgatg gtgagacgag gggttcaacg ggcagcctgc
       61 ggcgcggttc aggattctga cggctttgtc tggtcgattg gaggggaacc tgaggccacg
      121 gcgtccatcg gtggaggggc agtccgcttt ccaaacaccg gcgggagctt ggcaaacttg
      181 tccttgcgct cgttcagcat gtcgtacgct ttcatttctg aaaaacccgg cttatgagtg
      241 ccgacgacct tggaggcaaa tgcggagagc aaccttgtcg cctgttgagc ctggcaaaac
      301 ggacagacgg tgtcttctgg tcgggcatgg accgattggg tcgcatcgaa ctgtttctcg
      361 cattgcccgc aacggtactc gtagattggc atcgttgctc tcctttatga tcgtggcgac
      421 cagcgcgaca acgcgccctt gaccacccga caaagtttcg cttattgtag cacacttccc
      481 cggtccggag actaggggtg tgaaggaggg agatgtcgat gtcgttgttg attcggaagt
      541 acaaggggca taacggaatg atgcaggagg agcggatcga cgatccggat cggatcgagc
      601 ggtacctgag gctcttcgaa aaggacgatg tgaagaaact cgaaaccggc gtgaaagtct
      661 tcatcgaaaa ggatgaatgg cagctcctgc cctgaaccgg taaggtggga taggataagg
      721 gcttcagtca agatctcaaa tcaacctaca tcccttaccc tcacacctca ccgccagcca
      781 gtccccatga tctattggat tcatatcgcg cgaggaattg accgcgtaac cttgcacaag
      841 gaccgatgtt cggaggtgcc ctcaacccat ctgtcgagcg atttctggga agggggctgg
      901 ttcgactatc ctgacaagga acgcgcactg ttagccatgg aacaggcggg gacgaacgag
      961 cagcgacgct gtcccctctg caaaccatag acatgaagtg ctgagtgctc agtgctgagt
     1021 tgagcaccag acactcacca acccgaactc attactcagt actttgtatt ccccatgccc
     1081 cgtttagcac tccttctcac cacccttatc tggggtgcga cctttcccgc aacaaaagtc
     1141 gtgctggagc agattccccc tttatccttt ctgtttcttc gattttcctt gggggctttg
     1201 ttggttgtga ttgggatgct cctgtcaggg tttccagtcc gtcgcgagcg atcggttctt
     1261 cgggccagcg cgattgccac ggtgtggctc tttctgggct atctgcttca gactgtcggg
     1321 ttgtcgtata cgaccgcctc gaattccgca tttattaccg ccctctatgt ggtgttcgtg
     1381 cccctcatcc tgcgcaggtt cggttggcgt gtctgggtcg cgacgagtat cgcgatgggg
     1441 ggactctggt tgctggtcaa gccgacgatc gcgatcaatg ccggcgacct cctcacgctc
     1501 ggctgtgcgg tggcgttcgc ggcgcacatc gcctgtctcg aacgcttcac tcgtgaggtg
     1561 gatgcgcctt cgttgtttac ctggcagatg gtggcaatgg cggtcatctt gtgtccagcg
     1621 atggtggtcg aagaggcgcc agttgagtca tttgccccga cggcagtgtt gttggtaggc
     1681 ctagcgatta caggaggctt agcgacggga gcattcgcag tgcaaatgtg ggcacagcgg
     1741 ttgattccag cgcaacaggt cgcgttggtc tttgcgtccg aaccggtcta tgcggcctgg
     1801 ctgtcttggt attttctcgg cgaatcgttg gacctccaag gatggatcgg aagcggactg
     1861 attgtggctg ccgtcgtcat aggcgcgttt gcaaccgagt cgcatggagc ggagaagccg
     1921 gtcgcatcct tgcagacggc gtaggtgatc gagcggaagt ttacggggga agcgtgtcac
     1981 aaaaattcgg gaacagtcgt tgggtgcggg aagggtatct cgataatcgt gtcgagggaa
     2041 ccgtggtagg gcggattcac ttcgcggtaa tcggcgcggt cgatgtgtat ttgggaggga
     2101 atttcaaatc ggacattgcc gggcaggtca ttcaatttcg aaaccggcga ttcgaggagg
     2161 aggacgtggc cgggcaggtc atcggtgata tggagaatcc tcagatcggg acggtcaatt
     2221 tgatctcctt cgatcctcac cccaatctgg tgccccatcc ctacatcgag tggttttcga
     2281 tccgcaagaa tcattatcgg atcgaattgg aacctgccga tgcctgggtg atgtcggaat
     2341 ctgaagtggc gaccattgag tcagagagcc agcgtattcg ggagacgctg gctctgaggg
     2401 aatcggaacg tccctctcac gaagaatccg attgggtcta accgagtgtt gaagcagttg
     2461 gccgttgcgc gaggacggac tttgtgacca ctctgctggg agagccggct cgattcacgg
     2521 gagcttagac ggcgatggct tcctgcttgg tcttaccgtc gcggacatat gccgggacat
     2581 cgcggatatc ccagacgaga ccgagccact ccatgagctt caagccgtag taggtgatgt
     2641 cgacttccca ccaataaaag ccctggctgg ttgaagctgg gtaatagtga tggttgttgt
     2701 gccagccttc cccgagcgta atcaacgcga ggaaaaaatt atttcggctg tcgtcgcccg
     2761 tgtcgtaacg ctgcgagcca tagacatgag acagcgagtt gattgtgcag gttccgtgaa
     2821 ttaacacgac agtcgagata aagaaccccc aaatcagcat ctgcgggcca ttggtgccca
     2881 agcttggtgc gtaggtttcc aagagcctgc ccagaccgaa catcgcaaac ccagcgatgg
     2941 tggggaccag gatatcgaat cggtcgagaa atcgcagctc agggtacttg gcaaagtccg
     3001 ctatcgcctt cagtcgcggc gcgaaatgtg tttgcgaggt aaaccacccg atatgcgacc
     3061 acagaaatcc cccttggcgg ggcgagtgca cgtcgtccgg catatccgat gcgatatggt
     3121 gatgacggtg gtgactggcc caccagagcg gcccgcgctg ggcgcatgat ccgcctaaga
     3181 tggcaaaggc gagttgacag gcgcgagagg ttttaaaagt gcgatgggaa aaatatcggt
     3241 gataccagcc cgtaatggca aacattcgaa cgaaatagaa cgcggcggcc acggcaatcg
     3301 cggtccaact ccatcccacc caaatcacgc cgagacacat caggtggacg gcgataatgg
     3361 gaatgcagcg gagccagtcc atgcgaggag gtccgtcgac cttcaaatat tcgagtcccg
     3421 cccaggaatc gacccaccga agaatagtga accagagggc ctgggctttg ctcggatgta
     3481 ggacacgcac gtcggacatt cgaatacctc caaagtcagg gattgcaggc aggtaatgta
     3541 taaatacgcc gaatcccgat tcgcttctgt aatgtcgcgg cgctaagtgg cgattaatca
     3601 ggcaggccgc actctaatgg ggacgtatta tatacgtaat tgcctccgaa atggcaagaa
     3661 gatttttgcg cacgtagacc aagcatcagc ctgattgtag cggagccatt ggggttgtcc
     3721 accttttgtt gctgttgacg caatcagggc ttctacggag gctgaccggc tgcgtgcctc
     3781 ttgtttgaca ccctccactg aggcccccta gaatgcctcc ccgatgaatg accgttttct
     3841 caaagcctgc cgccgtgaac cggtggactg cactcccgtg tggttcatgc gtcaggccgg
     3901 ccgctacatg cctgaatatc gctcgcttcg agccaaacac tcgattctcg atctgtgcaa
     3961 gacgccggaa ctggcggcgc aagtcacgct tcagcccatc gatcgattcc cgctcgatgc
     4021 ggccatcatc ttcgcggaca ttctgcttcc gctcgaagcg atgggcctga gcctcgaatt
     4081 tgcggacggt gaaggacccg tcattcacaa tccgattacc gatcgcgggt ccgtagagcg
     4141 actcaaggtt atcgatggcg atgaactgtc gtacgtcgcc gacgcggtga gcctgacacg
     4201 ccaagcttta agcgggcgag tgccgttaat cgggttcgcg ggagctccgt tcaccttagc
     4261 cagttatgcc attgaaggcg gtggatcacg aaactacgtt ctcacgaagc agttgatgta
     4321 tcgggagccg gatgtctggc acaagcttat ggacaagtta gctcgtgtgg tgacgggcta
     4381 tctgcgccgg cagatcagag ctggcgccca agcgattcaa ctattcgaca gctgggtcgg
     4441 ctgtctcgcg cccggtgatt atgaagagta cgtccttcct cacgtgcaag ttgtttttga
     4501 ggggttgaag cgggaaggcg tgccgttgat ctatttcggt acgggaacgt cggccattct
     4561 tcgacagatg cgccgggctg gtggtgatgt gataggggtc gattggcgaa ttcatctcga
     4621 tgaagcctgg gccacggtcg gccatgatgt ggccgtgcaa ggtaatctcg atccgttggt
     4681 gctgttcgcg ccgctacatg aaatcgaacg ccgggtggaa gacatcctgc gtcgggctgc
     4741 cggacggccc gggcatatct ttaatctagg gcacgggatt ttgccgaata cacccgtcga
     4801 acatgtagct gctgtggtcg acatggtaca taagctcagt gcgagatagg ccacgaggct
     4861 gatgtgagct tcgagattgg aaccaatgaa gcggccgcga ggtgaattct ttgtatgccc
     4921 ggatcacgga aacccatcgc ggtgctgtta atggccatgg gagggcccga ctctctggag
     4981 aacgttgaac cgttcttgcg tgacgtgcgt ggaggtcgac caacgccgtc cgaacttgtc
     5041 gaagaaattc gggagcgata tcgcgtgacg ggtggaaaat caccggcatt cggaatcacc
     5101 aaagagattg cgaaaaagct ggaagagcga ttgaacgaac ctggcggtga acggtatcga
     5161 gtgtatgtcg ggctgcggca ttggcatccc tttattcacg agacctatgc cgaactgctc
     5221 agcgatgacc ctgaacaagt catcggtctc tgcatggcgc cccaacagtc atccttgagt
     5281 actggtgcct atcggaagaa agtggatgaa gtgcgagcct ccctgcagag tgattgtccg
     5341 gtgagc
//
LOCUS       DATDNW010000124         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_427193, whole genome shotgun sequence.
ACCESSION   DATDNW010000124 DATDNW010000000
VERSION     DATDNW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427193"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..596
                     /locus_tag="VI359_03140"
     CDS             141..596
                     /locus_tag="VI359_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973280.1"
                     /translation="MTTLKPILLAEDNPRDAELALAAMEEHHIADKVVVCHDGAEVLD
                     YLYCRGSYKTRRHGNPAVVFLDLKMPKVDGIEVLRTIKNDTDLQHIPVVMLTSSREER
                     DLAQSYALGANAYVVKPVEFHQFITAVKELGVFWGVINEPPPEGEASTQ"
     gene            615..2237
                     /locus_tag="VI359_03145"
     CDS             615..2237
                     /locus_tag="VI359_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973281.1"
                     /translation="MKSPLRLLLLEDNPVDADLITATMTEGGIPCQSHRVDTRQDFVA
                     ALKEGQIDLILADYSIPGFDGFTALTLARQHRPEIPFMFVSATIGEDLAIDAMHQGAT
                     DYILKQRLGRLVPSVQRALREVDERAERQRAEEALEQSEKQFRQAQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSQVLSTELGPEHPLRSKIEETQKAGERAAGLIRQLLAFSRKQSM
                     DPKVLSLNTVVTNLESMLRRLIGEDIRLVTKLDPGNGRVRADQAQLEQVLMNLVVNAR
                     DAMPKGGILTLETAQVELTRSPVHYLRPLPPGPYMKLSVSDTGCGMDRQTQSHIFEPF
                     FTTKEEGKGSGLGLSTVFGIVTQCGGGIDVASRVGHGTKFDVFFPSIESDVPLAAVPQ
                     DAGQPKRGTETILLVEDDPSVRNLVRDELRKLGYRVLEAKNGVEACLLATQQAGTLDL
                     LLTDVVMPGMGGRELAQHLSVIKPDLKILFISGYIDDVGLHVGHEEGSSSFLQKPFTP
                     EALARAVRELLDTRLRTDKTSATVPAPRAVLR"
     gene            2468..3235
                     /locus_tag="VI359_03150"
     CDS             2468..3235
                     /locus_tag="VI359_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973282.1"
                     /translation="MTVWTPQAPCQDVPPLVAIDIDPDRYRFVVHHYQREGLLTPPDI
                     RQWQKQTGHDLIFNAGLFREDFSYLGLLYANGRSIGSRRHPTWMGLFVAEPVDSTSRR
                     AKVLDLAVDPFDEQQPTYQEAAQSLMLLDRTGKVRVRQTGKRAQQTILAELGNGHMLL
                     LKSTDAVSLYAVGQCVRDTFPAVRQAMAMDGGSSSDVVLSAATAQTTSKAGVLQPWLA
                     QLGDNSTVHIGLPAVIGISPRHTASTNQTRQEESARR"
     gene            complement(3245..>3674)
                     /locus_tag="VI359_03155"
     CDS             complement(3245..>3674)
                     /locus_tag="VI359_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6973283.1"
                     /translation="VNMKSRAAFFVSASLLLLVAVSVVSAHHSYLLTVQQLRAGLTKA
                     PSITQKGFVLIDVRSAEEHATGYIPGTDLNIDFREIKARHREIGAQLDDHIVVYCQSG
                     HRSNIAAETLADLGYRHVYNVSGSMNAWQDAGFPIETARR"
BASE COUNT          818 a         1046 c         1040 g          770 t
ORIGIN      
        1 gacgagttcg aaggaacagg cattgggctt gcgaatgttc gacgaatcat tcatcgtcac
       61 ggtggcagga cccgggccga aggggcggtc gaccggggag cgacattttt ctttaccctc
      121 ccaacgaaaa gagctgtctg atgactaccc tgaaaccgat cttgctggca gaagacaacc
      181 ctcgcgacgc tgagctagct ttggccgcga tggaggaaca tcacatcgcc gacaaagttg
      241 tcgtctgtca tgatggggca gaggtgttgg attatttgta ttgccgagga tcttataaga
      301 cacgacggca tggcaatccg gcggttgttt ttctcgatct caagatgccg aaagtggatg
      361 gaattgaggt gctgagaacc attaagaacg ataccgacct ccagcatatt cccgtcgtca
      421 tgctgacgtc gtcgcgcgaa gagcgggact tggcacaaag ttacgccctg ggagctaacg
      481 cgtatgttgt caagcctgtt gaattccacc agttcatcac cgccgtgaag gaactaggcg
      541 tcttttgggg tgtgatcaac gaaccacctc cggaaggcga ggcatccact cagtagtctc
      601 gcaagcgagg acccgtgaag tccccactgc gtctcctgct tctcgaagat aatccggtgg
      661 acgccgacct gattacggcg accatgacgg agggaggcat tccctgccag tctcaccgcg
      721 tcgatacgcg tcaggatttt gtggctgcac tcaaagaggg ccagatcgat ctgattcttg
      781 ccgattactc gatcccaggt tttgatggat tcactgccct gaccctcgct cggcaacacc
      841 gcccagaaat acccttcatg ttcgtctccg caaccatcgg cgaggatctg gccattgatg
      901 ccatgcacca aggcgcgacg gattacatcc tgaagcaacg gttaggtcga ttggtcccct
      961 ccgtccagcg cgccttacga gaagtcgatg aacgggccga acgacagcgc gcggaggagg
     1021 ccctggagca aagcgagaag cagttccgac aggcccaaaa aatggaggcg gttggcagac
     1081 tggcaggagg catcgcgcac gacttcaaca atcttctcac tgttatcatg ggatacagtc
     1141 aggtcctgtc gacggaactg ggccctgaac acccacttcg cagcaagatc gaagaaaccc
     1201 agaaagcggg ggagcgagcc gccggattga ttcggcagct actggcattc agtcgcaagc
     1261 agtcaatgga cccgaaagtg ttgagtctca acaccgtcgt caccaatctg gaaagtatgt
     1321 tgcgccgttt gatcggggaa gatatccgcc ttgttactaa gcttgatcct ggaaacggcc
     1381 gcgtgcgggc cgaccaggcg cagctcgagc aggtcctcat gaatcttgtc gtcaatgcac
     1441 gagacgcgat gccgaaaggc ggaattctca ccctcgaaac cgctcaagtc gaactcaccc
     1501 gtagccctgt ccattatctc cgcccattgc ctcccggccc ctatatgaaa ttatcggtca
     1561 gcgatacagg gtgcgggatg gatcgccaga cccaatcgca tatcttcgaa ccgttcttca
     1621 cgacgaagga agaaggaaag ggtagtgggc ttggtctctc cacggtgttc ggaatcgtta
     1681 ctcaatgcgg tggcggcatc gacgtggcca gccgcgtggg acatggaaca aaattcgacg
     1741 tgttcttccc gagcatcgaa tccgatgtgc cactcgccgc tgtcccacaa gacgctgggc
     1801 aacccaaaag gggaacggaa accattcttc tcgtagaaga cgacccgagt gtccgcaatc
     1861 ttgtgcgaga tgaactgcgt aaactcggtt atcgggtgct ggaggccaag aacggcgtcg
     1921 aagcctgctt gttggctaca cagcaggcgg gcacgctcga tctgttgctg accgatgtcg
     1981 tgatgcccgg tatgggcggc cgagaactgg cccagcacct ctccgttatc aaaccggatc
     2041 taaaaattct cttcatttcc gggtacatcg acgacgtcgg tctccatgtg ggacatgagg
     2101 aaggctccag cagctttctc caaaagccct ttacccctga agctcttgcg cgggctgtta
     2161 gagagctcct cgacacgcga ttgcgcactg acaaaaccag tgccaccgtg cccgccccac
     2221 gagcggtttt gcgctgaaaa cacggcttta cacgtgtcgg ctttaaaaac tgcgtgattt
     2281 gacggtccgc gcatcgcctc tctatactca gccgcaacga accatgagat accgctttca
     2341 gtgtcgccga tcctgctcaa gctttgctgt cgtgcttttg ctcggaacgg catggctggt
     2401 cattggctcc ccataccagg caactaccca aacattgacg tgggatcctg tcgcggaagg
     2461 gctgtccgtg acggtctgga cgccgcaggc accctgccaa gacgtcccgc cacttgtcgc
     2521 cattgacatt gacccggatc gctatcgctt tgtggtccac cactatcagc gggagggact
     2581 attaacccct ccggatattc gtcaatggca gaaacagaca ggtcatgacc tcattttcaa
     2641 tgccggtcta ttccgtgagg acttttcgta tctcggcctg ctgtatgcca atggacgctc
     2701 gatcggcagt aggcgccatc ccacgtggat ggggcttttc gttgccgagc ctgtcgactc
     2761 gacctcgcgt cgggcaaaag tgttagacct tgccgtcgat ccgtttgacg agcagcagcc
     2821 cacgtatcaa gaagcggcgc aatcgctgat gctgctcgac cgaacaggaa aagtgcgcgt
     2881 gcgccagacg ggcaagcggg cccagcaaac aattctcgcg gaactcggca acggacacat
     2941 gctcctgctc aagagtacgg atgcagtgtc actctatgcc gttggccaat gtgtgcggga
     3001 tacatttccc gcggtccggc aggcgatggc gatggatgga gggtcttcat cggacgtggt
     3061 cctgagcgcg gcaaccgcac agacaaccag caaagcaggt gtcctgcagc catggctcgc
     3121 acagttgggc gacaactcga cagtccatat cgggctcccg gctgtgatcg gcattagccc
     3181 gcgccacacc gcatcgacaa atcagactcg gcaggaagag tctgcgcgtc ggtaacggtc
     3241 caagttatcg acgcgccgtc tcgattggga accctgcatc ctgccaggcg ttcatactgc
     3301 cgctgacatt gtagacatgc cggtatccca gatcggccaa ggtttctgcg gcgatgttgc
     3361 tgcgatggcc cgactgacaa tagacgacga tgtggtcgtc taactgagct ccgatttcgc
     3421 gatggcgcgc cttgatttcc cggaaatcga tattgaggtc cgtgccgggg atatagcctg
     3481 tcgcatgttc ttcggcggat cgcacatcaa tcaagacgaa gcctttctgt gttatagagg
     3541 gagctttcgt caatccggcc ctcagttgtt gaaccgtcag cagatacgaa tggtgggcgg
     3601 agacgacgct gaccgcaacg agcagtagaa gcgaggcgct gacgaagaag gcggctctcg
     3661 atttcatgtt cacc
//
LOCUS       DATDNW010000125         5559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_697088, whole genome shotgun sequence.
ACCESSION   DATDNW010000125 DATDNW010000000
VERSION     DATDNW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697088"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..834
                     /locus_tag="VI359_03160"
     CDS             220..834
                     /locus_tag="VI359_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY6973284.1"
                     /translation="MKTMASVLLAGLLTVLQATPALADGEFEPSSVARGVLLVASPSL
                     ADPNFREAVILIVEHGSEGTLGLILNRSTHVLLSEALPDLTVLKGTSYRLFAGGPVEP
                     TRLLLLSRLKEPPADVRPVFDGVYVGGTPEVLERIITQAKPTETFRAFAGYAGWAPGQ
                     LAFEMLEGSWAVLPPDSFNIFDKDSATLWSDCISRLQAPRVISY"
     gene            complement(907..1311)
                     /locus_tag="VI359_03165"
     CDS             complement(907..1311)
                     /locus_tag="VI359_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973285.1"
                     /translation="MGRRLLIIDNEPHLCESLRDRLEAMGYTVIVAHDGRTGLALMAL
                     EAKQTPIRGVLLDLQMPVMDGIQVLRELRVQHEQVPVVMMTAEPDRRIREEALSLGAK
                     HYILKPVDATLLTQICKQTFPLTNHDGVDLLD"
     gene            complement(1286..1567)
                     /locus_tag="VI359_03170"
     CDS             complement(1286..1567)
                     /locus_tag="VI359_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973286.1"
                     /translation="MPNEADSGIHHFEYAEQESPIVALLRSSGKLTVEEIVARIPDLS
                     WSQLFLAVDLLSRTKDVILRREGFTYTLEMAKPSPVLAGASHGTTASDH"
     gene            complement(1870..2298)
                     /locus_tag="VI359_03175"
     CDS             complement(1870..2298)
                     /locus_tag="VI359_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY6973287.1"
                     /translation="MSKPSTVHIRFPVAIFLLCSGMLLIGSAAAAFALADPVEIAFET
                     TAPYYQPRVAVVSAGAPVQWVNTTGSPHSVRHDGCVTDETCAFQSIAVPPDSSFLIAP
                     LPPGRYGYHCELHPIMRGTLVVVDSLARGDGIHPALEPTR"
     gene            complement(2484..3194)
                     /locus_tag="VI359_03180"
     CDS             complement(2484..3194)
                     /locus_tag="VI359_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973288.1"
                     /translation="MTQGARILVVDDEPQIRRSLQVNLERSGYAVETVETGEAALSSF
                     RNRRPDVVILDLIMPGMGGVEVVRRIRESSTVPIIVLSAMGEEARKVDALELGADDYM
                     TKPFGMHELLARIRSLLRRAAGAHSPDPVFMTGDLCVNFDRREVTLDQKPVKLTPTEY
                     DLLKFMIEHAGKVLTQRMLLQEVWGQAYVDQAQYLRVFIGQLRKKLEKDPTRPRFILT
                     DPGVGYRFCTERDSPSVT"
     gene            complement(3191..4618)
                     /locus_tag="VI359_03185"
     CDS             complement(3191..4618)
                     /locus_tag="VI359_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973289.1"
                     /translation="MAAAASPPSVRSYDVNWTVAGILGLLIITALAIVPLTLTVIHSR
                     ESDALVIDLAGQQRMLLERHMKEVLLASQGVDAQYQQTRAALKDRVQTLIHGGSTMAQ
                     VNRDGTLAVPAAPTDTIRQTLLEQQRLMEALFTNADAFLLAARTGEQRGAARDDLLRD
                     NAALLAIANDVVTQLTKHSEDNVQTLIRWELVVVLMVLTVATLGTWRFLRAEQALKRS
                     QAMTVQALHQRDAVKSSLLSSVSHELRTPLTAIKTMVFSLGHDRESLSPSIRHEFLAG
                     IDRELDYLNRLIGNLLDMSRLEAGTLAPRREWHVLDELVEGAIRRVEPLLAGRPLHVL
                     LAPDLPPIPVDGMQFQQVLVNLLDNAIKFSPPESPIRLTAALAGNSLEVRVSNTGEGI
                     PPNELDRIFDRFYRVQSGRSSGPPGTGLGLAICKGIVEAHGGSILAQSVPGGETTILF
                     RIPVAASPPAGDRAESEPQLVRRAS"
     gene            5334..>5559
                     /locus_tag="VI359_03190"
     CDS             5334..>5559
                     /locus_tag="VI359_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973290.1"
                     /translation="MPLIPEPLNHRLVQLRRVLHQYPELSGQETNTAAVICKFLDGLA
                     IKYRAHVAGHGVVADIPGRAGVPCIVLRADT"
BASE COUNT         1149 a         1648 c         1693 g         1069 t
ORIGIN      
        1 aacacgatgt acctggtcgc actggacgat gcgcacacgg gttcactcgt caacgccgtg
       61 cgagagctgc atgccaggtt ggtgaaagaa aacgacggtc acgaggtccg gctcaaagtc
      121 ttcgtccatc cctgtgagat gatcgtgtag tcatccggga gccaaatccc catgctgcct
      181 ccgtctgagt cctcgagcgc actgagccct gccgaggcaa tgaaaaccat ggcgagcgtc
      241 ctgctcgcag gacttttgac cgtcctgcag gcaacaccag ctctggcgga cggggaattc
      301 gagccgtcgt cggtcgcgag gggtgtactc ttggtggcca gcccgtcact ggctgatccg
      361 aacttccgcg aagccgtcat cctcatcgtg gaacacggtt ccgaaggaac ccttgggctc
      421 attctgaatc gttccacgca cgtcctcctg tctgaagcgt tgccggacct cactgtgctc
      481 aaggggacca gctaccggct gtttgccggt gggccggtcg agccaacccg cctgcttctg
      541 ttgtcccgac tcaaggagcc tccagcagac gtgcgaccgg tcttcgacgg ggtctatgtg
      601 ggaggcacac ccgaggtgct ggaacgcatc attacgcagg ccaaaccaac cgaaacgttc
      661 cgggcctttg ccggatatgc cgggtgggct ccggggcaac tggcatttga gatgcttgaa
      721 gggtcgtggg ccgtcttgcc gccggattcc ttcaacattt ttgacaagga ctcggccaca
      781 ctctggtcgg actgcatcag ccgcctccag gcgccgcggg tgatctccta ttaaagggag
      841 aggggatgga gattgttgat ctcctgtgtt cgcgcaacct gcctacctga cgaacacaat
      901 cgcaattcaa tcgaggagat cgactccatc atggttggtc aggggaaagg tttgcttgca
      961 gatttgtgta agcagggtcg catcgaccgg cttcaagatg taatgtttag ctcccaggct
     1021 gagcgcctct tcccgaatcc tgcgatctgg ctccgcggtc atcatcacaa ccggcacctg
     1081 ttcatgctga actcgtagtt ccctgagcac ttgaatgccg tccatgacgg gcatttgcag
     1141 gtccagcagc acgcccctga tcggagtctg cttcgcttcg agggccatga gcgccagtcc
     1201 cgtccgtccg tcgtgagcga caatgacggt atagcccatg gcttcaagtc ggtccctcaa
     1261 gctttcacag agatggggct cgttatcaat gatcagaagc cgtcgtccca tgtgacgctc
     1321 cagccagaac cggagatggt ttcgccattt ccagggtata ggtaaatccc tcccgacgaa
     1381 gaatgacgtc cttagtgcga ctcagcaggt ccacggcaag aaagagttga ctccaactga
     1441 ggtccggtat ccgtgccaca atctcctcga ccgtcaattt gcccgaagaa cgtaggagag
     1501 ccacgatcgg ggattcctgt tctgcgtact cgaagtggtg aattccagag tcagcctcgt
     1561 tcggcatggg gtcctccttt cttttctcag acgttcgggt cggcaatgga acggtcatgg
     1621 gtcgacaagt gcgctcacgg tctgcgccgt gcaaccgaga cgattcgcca cgatgacggg
     1681 atcgatgatc ccgccacatt gcacgcagct ccatgcgtgg gccgtttgtg tctctgtgat
     1741 ccacccccac tctccatacc gaaccggaat caggaatccc tggcagcgtg ggcagtgagc
     1801 aggccgcacg gcggtcggtt caggctccat cggtggtggt gaggacggcc gttcgttcag
     1861 cacactcgtt cacctggttg gttccagggc gggatgtatc ccgtcgcccc gcgcgagtga
     1921 atccacgaca acgagcgtgc cccgcatgat ggggtgcaac tcgcagtggt agccatagcg
     1981 tcccggcggc agcggagcga tgaggaagct gctatccggc gggaccgcga tcgattgaaa
     2041 cgcgcacgtt tcatccgtca cacatccatc atggcggacg ctatgaggag atcccgtcgt
     2101 attgacccac tgcacagggg cgcctgccga cacgactgca acccgaggct ggtagtaggg
     2161 cgcggtcgtt tcgaacgcga tctcgaccgg atcggccagg gcaaacgcag ccgccgcgct
     2221 gccgatcaag agcatgcccg aacagagcag aaagattgca accgggaagc gaatgtgaac
     2281 ggtggagggt ttgctcatcg tgccactcct ttccacgagg gattcgcatc gatctggcgc
     2341 gtagatacaa taccgccgga gaggataagg actcgatatg tggatgggtg gaatcgtata
     2401 aagaacgcgt aaagatcagc cgcgcgcgat ctccacgcga atcggagagg gaagaaatcg
     2461 ggggattgaa gacggaaccg cgttcaggtg accgatggag aatcgcgttc ggtgcagaag
     2521 cgatacccga cgccggggtc ggtcaggata aaccgtgggc gagtgggatc tttttcgagc
     2581 tttttccgca gctgcccgat gaacacgcgc agatattggg cctggtccac ataggcctgt
     2641 ccccacacct cctgcaacag catccgttgc gtgagcactt tgccggcatg ttcgatcata
     2701 aatttgagca ggtcatactc ggtcggcgtc agtttgaccg gcttctgatc cagcgtgacc
     2761 tcgcgccggt caaaatttac gcagaggtcg cccgtcataa acaccgggtc cgggctatgg
     2821 gcgccggctg ctcgccgtaa caacgagcgg atccgggcca acaattcgtg catcccgaag
     2881 ggcttcgtca tgtaatcatc cgcgcccagt tcgagggcgt cgaccttgcg ggcctcctcg
     2941 cccattgccg agagcacgat gatcggcacc gtggaggatt cgcgaatccg gcgcaccacc
     3001 tccaccccgc ccatccccgg catgatgaga tccaggatga cgacgtcggg acgccggtta
     3061 cggaatgaac tgagcgccgc ttcgcccgtc tccacggttt cgaccgcata gccgctgcgt
     3121 tcgagattga cttgaagcga tcgccggatc tgcggctcat cgtcgacgac gagaatgcgc
     3181 gcgccctgtg tcacgacgcc ctccgcacga gttggggttc ggactccgcc cggtcgccgg
     3241 cgggcggcga ggcggccacc gggatccgaa acagaatcgt cgtctctcca ccaggaaccg
     3301 actgagcgag aatcgaaccg ccatgggcct cgacgatgcc cttgcagatc gccaacccga
     3361 gaccggtgcc cggagggccg gacgaccgtc cggactgcac acggtagaat cggtcgaaga
     3421 ttcggtccag ttcgttggga gggatcccct cgccggtgtt tgacacgcgc acttcgagag
     3481 agttccccgc gagtgcagcg gtgagccgga tcggagactc cggcggagag aacttgatgg
     3541 cattgtcgag gagattcacg agcacctgct ggaactgcat cccatcgaca gggatcggcg
     3601 ggagatccgg agccagaagc acgtggaggg gccggcctgc cagcaacggc tcgacgcgtc
     3661 ggatagcgcc ttccaccagt tcatccaaga catgccattc ccggcggggt gccagcgtcc
     3721 cggcctcgag acgggacatg tccaagagat tgccgatcag cctgttcaaa taatccagtt
     3781 cccggtcgat gcccgcgaga aactcgtggc ggatggaggg cgagagcgac tctctgtcgt
     3841 gcccaaggct gaacaccatc gttttgatcg ccgtgagcgg cgtgcggagc tcatgcgaga
     3901 cggaagacag caacgaggat ttgaccgcat cacgctgatg cagggcttgg acggtcatcg
     3961 cctggctccg tttcaatgcc tgttccgctc gcagaaagcg ccaggtgccc aacgttgcca
     4021 ctgtcagcac catcagaacc accacgagtt cccaacgaat gagcgtctgg acattgtcct
     4081 ccgaatgctt ggtcaactgc gtcacgacat cgttcgcgat ggccaagagt gcggcattgt
     4141 ccctgagcaa gtcatcccgc gccgcgccgc gctgttcgcc ggtccgtgca gccaggagaa
     4201 aggcatcggc gttggtgaac aacgcctcca tgagtcgttg ctgttcaagc agcgtctggc
     4261 ggatcgtatc ggtcggggcg gcgggaaccg ccagagtccc gtcgcgattg acctgggcca
     4321 tggtcgagcc cccatggatc aacgtctgca cccggtcctt cagcgcggcg cgagtttgct
     4381 ggtactgagc gtccacaccc tgagaggcga gcaggacctc tttcatatga cgctccagca
     4441 acatgcgttg ctggcccgcc agatcgatga cgagcgcgtc ggactcgcga ctatgaatga
     4501 cggtcagggt gaggggaacg atggcgagtg cggtgatgat cagcaggccg aggatgcccg
     4561 cgaccgtcca attgacatca tacgatctca cagagggcgg ggatgctgcg gcagccacga
     4621 cgacctccaa tcggttccca tcatatcaat gagcccaggg ggaagggtag cctggttagt
     4681 cccctgtgct cacggaaccc ccacgataaa aatgatatgg aacaacgccc atgctgctcc
     4741 tttgctcccg gagagcgcac gcgggactaa tcagatttct aaattagggg cggtgctccc
     4801 tcgacggccg cagtaggagc agcacaggcg ttattccatt gaagtgaagt acgagcaagc
     4861 tttgaaggaa cattaagcgt gcgcagttgg ggactaatcc aggccaccct ttgaggagag
     4921 gacgagcaag cttggaggga ccataatata tcgttcgacg cgcgcagtga ggtgactacc
     4981 caagccaccc tatgaaggga gaaacgagca agcttggaag gatcatttgt atcggtcaat
     5041 gcgcgcagtt gagggataga tccagaccac ccccttgaga ggaggtacga gcaagcttgg
     5101 agggaccatt atagtggtcg ttcgacgtgc gcactccagg gtcgaccaag tcaccctacc
     5161 tgggagagga gcgagcatag caagagcacc atccaggcag tggcaacccg ctgcagaaac
     5221 cgtggtcggg tctgtgctag catagctgtg ttatcgtcag atcgccggtc tggccggacc
     5281 cggcgaaggg tacgcctaca ggccgtcgta ggttcactgc caaggttgca atcatgccat
     5341 tgattcctga accgctcaat caccgtctcg ttcagcttcg acgagtgctc catcagtatc
     5401 ccgaactgag cggacaggag acgaataccg ccgcggtgat ctgcaaattt ctcgatgggc
     5461 tcgccatcaa gtaccgggcc cacgtcgccg gccacggagt cgtcgccgac attcccggac
     5521 gcgccggagt gccctgcatc gtgttgcgtg ccgatacgg
//
LOCUS       DATDNW010000126         3990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_153955, whole genome shotgun sequence.
ACCESSION   DATDNW010000126 DATDNW010000000
VERSION     DATDNW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153955"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VI359_03195"
     CDS             <1..562
                     /locus_tag="VI359_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6973291.1"
                     /translation="SVNHQQGGAAMFKQLKEQKGFTLIELMIVVAIIGILAAIAIPNF
                     LAYQARSRQAEARTNLGAVFVSQVAFFGENNRYSDFATIGYTLAGQSNRYTYRSPNQA
                     GNAGSTGAQGADVFATLAGSATAGGVITAEPGGTTPSNATLPAAGVAGAFTATASGNV
                     DGDATTDHWLVNDVKAIANDLNDVTS"
     gene            complement(1056..1385)
                     /locus_tag="VI359_03200"
     CDS             complement(1056..1385)
                     /locus_tag="VI359_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6973292.1"
                     /translation="MINKQDTEAPAKVTVYIDPDLAEIVPGFLENRRRDVQFLQTALQ
                     QDDLTTIRVLGHRMKGDGGGYGFDTISAIGEALELAAVQQDRLTIERQTSELNNFLTR
                     LDVVYRR"
     gene            complement(1382..>3990)
                     /locus_tag="VI359_03205"
     CDS             complement(1382..>3990)
                     /locus_tag="VI359_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973293.1"
                     /translation="KKIPIVDEQGRPLYLLGISEDITDQKHQHETLVRLQRAVEHGME
                     GLALLNADGLYTYMNPAHAALFGFEVEELIGKSWKELYGPEQQATIEQYHFPFLQVDG
                     YWRGELVGRKKTGELFDVEVALQQFAGSDGHTSEIIWTCRDITDRKQVTRQEKRRFSL
                     LQEHQIGLLKLAQSEAIISGNLQEAFQAITEVASRVLAVERASIWLFQEDRSAIHLAD
                     LYETANGRHSAGSVLHGYQFPSYFQALEQEDYVIAAHDAHCDPRTREFSSAYLTPFGI
                     GAMLDAPIRHKGKVVGVLCHEYLGMSRTWTADELTIAVSFATMTTLAMEAMERGQAER
                     AVRQAKEKAETANRAKSEFLASVSHEIRTPMNAIIGMADLLWETELTPDQRKYLRIFR
                     RAGSNLLNLINDILDFSKVEAGHLELESIAFDLGDLIEKAIEILAMRANEKGLELACH
                     LSPDVPCALIGDPNRLQQILINLISNAVKFTEKGSVTLRIQNDPDRAAPGAIRFSVTD
                     TGIGIPSEKLATIFESFTQAHASITRKYGGTGLGLTISKQLSERMNGRMWVESTEGQG
                     STFYCSVQLGIQPQDPLANLPLPINLAGLRTLVVDDHATNRLILRETLTAWGAQVTDV
                     ADGAAALDALHQASESARPFELAFLDCRMPDMDGFQVVEKIREAKLAEGLTIVMLTSD
                     HWADDIARTYDLRLGGYLIKPIRRSDLLQTISIVLARGKGIQPAGLPEPSRPSADSSM
                     NKLRILLVEDSPDNQVLIRSYLKPTSHRLDIADNGAIAFEMFKTGHYDLILMDMQMPV
                     MNGYDTTRAIRAWEREHDLPRTQIIALTALALKEESAKILDSGCDAHMTKPIKRATLL
                     EVLQAYRGNLAA"
BASE COUNT          827 a         1059 c         1149 g          955 t
ORIGIN      
        1 gtcagtgaac catcaacaag gaggagcagc aatgtttaag cagttgaagg aacaaaaggg
       61 ttttacgttg atcgagttga tgatcgtggt ggcgatcatc gggatcttgg cggcgatcgc
      121 catcccgaac ttcttggcgt accaggctcg gtctcgccaa gctgaagcca ggaccaacct
      181 gggcgcggtg tttgtgtcgc aagtcgcctt cttcggtgaa aacaatcggt actcggattt
      241 tgcgacgatc ggctataccc tcgccggtca atcgaaccgg tacacctacc ggagtccaaa
      301 ccaggctggc aacgcgggaa gcactggtgc gcagggtgcg gacgtattcg caacgcttgc
      361 cgggtccgcg actgccggtg gtgtgataac agcggagcca ggcggcacga cgccgtcgaa
      421 tgccacgtta cctgcagccg gtgtggctgg ggccttcacg gcgacggcat caggaaatgt
      481 ggatggcgat gccaccacag accactggtt ggtgaacgac gtcaaggcga tcgcgaacga
      541 cctgaacgac gtgaccagct aagtgatctt cagtatgcgg tgatgctgtg gcggggagag
      601 agcgatgctc tccccgcctt ttttctttct cctgcgccaa tatgaaagct aagggccgtg
      661 aacgcagagc ctcttgcctc ccctacgtca gaggtcaccg ctcccttgta gccactcatg
      721 agcccgaaag aagttagtat aaagggttct catcagttca ggctaatgcg gttggtgcgt
      781 ccttgggaat gttgggaagg ccgaagaggg acgcgaatcg tgggcctaaa taaagcaggt
      841 agcttgtgcc ctccaccaaa cctcgcttgt gcgcgccgcc ggctaagtgt cgttgcatca
      901 cacgtgatgt aatgctcgcg gataaaacct gcaatcgtac agcagaatcg ctccctcgta
      961 ccggcctctt atagccttat cgaacgtggg tcgacagata aacacattca gtgccgcatt
     1021 ctcactgaac atattctgca gttgttccct gcttgctatc tccgatagac cacatcaagg
     1081 cgggtcagga agttgttgag ttctgaggtc tgacgctcaa tggtgagtcg gtcttgctgc
     1141 acggcggcca gttccaatgc ttcaccgatc gcgctgatcg tgtcgaagcc gtaccctccg
     1201 ccatcgccct tcatccgatg accgaggacg cgaatcgttg ttaagtcgtc ctgttgcagt
     1261 gccgtctgga ggaattgcac gtcacgccga cggttctcaa gaaacccggg aacaatctcg
     1321 gcaaggtcgg ggtcgatata taccgtcact ttcgctggag cttctgtgtc ttgcttgttg
     1381 atcatgcggc caggtttcct ctgtaagcct ggagaacctc caggagcgtc gcacgcttaa
     1441 tgggctttgt catatgggca tcgcagccgg agtcgagaat cttggcactt tcttctttca
     1501 atgccagtgc cgtaagggcg atgatttgtg ttcgagggag atcgtgttcg cgctcccacg
     1561 cacgaatggc cctggtggtg tcatagccgt tcatgaccgg catctgcata tccatcaaaa
     1621 tcagatcata gtggccggtt ttgaacatct cgaaagcaat tgcgccgttg tcggctatgt
     1681 caaggcggtg tgaagtcggt ttcaagtagg aacggatcag cacttgattg tcaggggaat
     1741 cttccaccag tagaatccgt aatttgttca ttgaggagtc agcggagggc cgagatggtt
     1801 caggaagtcc cgccggctgg atgcctttcc ctcgtgccag cacaatgctg atggtctgca
     1861 acagatcgga gcgtctgatc ggcttgatga ggtagccgcc caatctcaga tcataggtcc
     1921 gtgcgatatc atcggcccaa tggtcggagg tgagcatcac gatggtcaat ccctcggcca
     1981 gtttggcctc tcgaattttc tccacgactt gaaagccgtc catatcgggc atgcgacaat
     2041 cgaggaaggc cagttcgaag ggacgagcgg actcggatgc ttgatgtaat gcatcgaggg
     2101 ctgctgctcc atcggccacg tcggtgacct gggctcccca tgcggtcaaa gtttctcgga
     2161 ggatcaggcg gtttgtcgca tggtcgtcga cgacgagtgt tctgagcccc gccaggttga
     2221 tcggcagcgg gaggttggcg agcgggtcct gcggttggat tcccaactga acgctgcagt
     2281 aaaacgtgct gccctgacct tccgtacttt cgacccacat tcgcccattc atccgctcgg
     2341 agagttgttt cgaaattgtc aacccgagac cggttcctcc gtacttgcgg gtgatggagg
     2401 catgggcttg ggtgaagctt tcaaaaatgg tggccagttt ttccgaggga atgccgatgc
     2461 ccgtgtcggt gacggagaat cggatagcgc ctggtgcggc gcgatcagga tcgttttgga
     2521 tgcgaagggt gacggatcct ttctcggtga atttcaccgc gttactgatc aagttgatca
     2581 aaatttgttg taaccggttg ggatctccga tgagcgcgca tgggacgtcc ggtgagaggt
     2641 ggcacgcaag ctccaacccc ttctcgttag cccgcatggc gagaatttca atggccttct
     2701 caatcaggtc acccaagtcg aaagcgatcg actccaactc cagatggccg gcttcgacct
     2761 tggagaaatc gaggatgtcg ttaatgaggt tcaggaggtt gctgcctgcc cggcggaaaa
     2821 tgcggagata tttcctctgg tccggtgtga gttctgtctc ccacaggaga tcggccatgc
     2881 cgatgatggc gttcatcggt gtgcgaattt catggctcac gctggcgagg aattcgctct
     2941 tcgctcgatt tgctgtttcg gccttctcct tggcctgacg gacagcgcgt tctgcctgac
     3001 ctcgctccat agcctccatc gcgagggtcg tcatcgtagc gaaggaaaca gcgatggtca
     3061 gttcatcggc agtccatgtc cgtgacatgc ccagatattc gtggcacaag acgcctacca
     3121 ctttcccctt gtgtctgatc ggcgcgtcga gcatggcgcc tattccaaac ggagtgagat
     3181 acgcggatga aaattctctg gtgcgaggat cgcagtgagc atcgtgagcg gcaatgacat
     3241 aatcctcctg ctctaaagct tggaagtaag acgggaattg gtagccgtga aggacagagc
     3301 cagcactatg ccttccgttc gcggtctcat acagatctgc cagatggatg gctgagcggt
     3361 cttcctgaaa cagccaaatg ctggcacgct caaccgccag cacacgactc gccacttctg
     3421 ttatggcttg aaatgcttct tgaaggtttc cggagatgat cgcctcgctc tgggctaatt
     3481 tgagcaaacc gatctggtgc tcttggagta agctgaaccg ccgcttttcc tgtcgtgtaa
     3541 cttgcttgcg gtctgtgatg tcgcgacagg tccagatgat ctcggaggtg tgtccatcgc
     3601 tgcctgcgaa ttgttgcaat gctacttcga cgtcaaacag ctcaccagtc tttttcctgc
     3661 ctaccagctc accacgccaa tatccatcaa cttgcaagaa aggaaaatgg tattgctcaa
     3721 ttgtggcctg ttgctctgga ccgtagagtt ccttccagga ctttccaatt agctcctcaa
     3781 cctcaaaacc gaacagggcg gcatgggccg gattcatata ggtgtacagg ccgtcggcat
     3841 tcagtagggc gaggccctcc atgccatgct caaccgctcg ttggagtcga accagggtct
     3901 catgctggtg tttctggtcg gtgatgtctt ccgatatccc gagcaagtac agggggcgtc
     3961 cctgttcgtc cacaatggga attttttttg
//
LOCUS       DATDNW010000127         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_153958, whole genome shotgun sequence.
ACCESSION   DATDNW010000127 DATDNW010000000
VERSION     DATDNW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153958"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VI359_03210"
     CDS             <1..1122
                     /locus_tag="VI359_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HEY6973294.1"
                     /translation="TSVDELTKLGQVNDWLKKSKLVAKAHEALGSRFKLGLVKVDLDF
                     KGAEAAAKEMIGRQVGSITVSQVIVSEMIPHKEEYYCAVKSTREGSEILVANCGGIEV
                     ESNWDRVKRLTLDVGQNPTPEGLDKLVKDAGFTGSLAKKMADFAGKMFVCFDNEDAQY
                     LEVNPVVVRESDGELVALDAVTLLDGDAKFRHPDWNFAFAAEFGRAYSENEMEVMAVD
                     SKIKGSVKFIEIPGGDTAMLPAGGGASVYYSDAVVARGGKLANYAEYSGDPPDWAVEV
                     LTDKVCSLPGIRNIIVGGAIANFTDVKKTFGGIINGFRKAKSEGKLNGVRIWVRRGGP
                     REKEGLDAMRALREEGFDIHVFDRNTPLTDIVDKALQTK"
     gene            1168..>2670
                     /locus_tag="VI359_03215"
     CDS             1168..>2670
                     /locus_tag="VI359_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY6973295.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKKPLNVEAFVY
                     PPDAGKTNEIPYGSGLLAIPIYKSIAEATKHHPSINTSLVYIGADRAMRGGMEALDDP
                     HIKLVSMITEGVPEKDAKLLGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVVTKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRIKLIGVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGAIVPPTFGALGPAIKEAYQELLKSGQVKEVVEPVVLP
                     KLPKPVEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVMGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGLIMIGPRF
                     GGAVTDAGRYFKHAVDNRMSVDEFLAYMKKSVGPVPGIGHRVKSLRNPDKR"
BASE COUNT          594 a          721 c          837 g          518 t
ORIGIN      
        1 acctcagttg atgaactcac caaactcggg caagtgaatg attggctgaa gaaatccaaa
       61 ctggtggcga aggcccatga agcgctgggg tcccggttca aactcggtct tgtcaaagtt
      121 gatctggact tcaagggcgc cgaggccgcg gccaaggaaa tgatcggtcg ccaggtcggg
      181 agcatcaccg tgtctcaagt gattgtctcc gagatgattc ctcacaagga agaatattat
      241 tgcgctgtga aatcgacccg cgaggggtcc gagatactcg tcgccaattg cggcgggatc
      301 gaagtcgaat cgaattggga tcgcgtcaag cggctcacgc tcgacgtggg tcaaaatccc
      361 accccggaag gactggacaa gctggtcaaa gatgccgggt tcaccggttc gctcgccaag
      421 aagatggccg attttgctgg caagatgttt gtgtgctttg acaacgagga tgcacagtat
      481 ctggaggtca atccagtcgt tgtgcgcgag agcgacgggg aactggtcgc gctggacgcc
      541 gtcaccttgc tcgacggcga cgcgaagttt cgccatccgg attggaactt tgcctttgcg
      601 gcggaattcg gtcgcgccta ttccgagaac gagatggaag tgatggcggt ggattccaag
      661 atcaagggct cggtaaaatt catcgaaatt cccggtggtg acacggcaat gctgccggcc
      721 ggcggcggcg ccagcgtgta ctattcggac gcagtcgtcg cgcgcggagg gaagctcgcg
      781 aactatgccg aatattcggg cgatccaccg gattgggcgg tcgaagtcct gaccgataag
      841 gtctgctcac tgccggggat caggaacatc atcgtcggtg gcgcgatcgc caatttcacg
      901 gacgtaaaaa agaccttcgg cggcattatc aacggcttcc gcaaggccaa atccgaaggt
      961 aagctgaacg gcgtcaggat ctgggtgcgc cgcggtggcc cgagggagaa ggaagggctc
     1021 gatgcgatgc gtgcgctcag ggaagaagga ttcgacatcc atgtgtttga tcgcaacaca
     1081 ccgctcaccg acattgtcga taaggcgctc cagacgaagt agcctgccac attgtccatg
     1141 accatactga cataacggga gatcccgatg agcattctcg ccaacaaaga cacccgcgtg
     1201 gtgattcagg gtggccaagc aggcgtcaac gctgcccgcc gcatggccga gttttgttac
     1261 ctgatcaaga agccactgaa cgttgaagcc ttcgtctacc cacccgatgc cggcaagacc
     1321 aacgagattc cctatggcag tgggctcctc gccattccca tttacaagag catcgccgaa
     1381 gcgaccaagc atcacccctc gatcaatacc agccttgtct atatcggagc ggatcgtgcg
     1441 atgaggggtg gcatggaagc gctcgacgat ccgcacatca agctcgtctc catgatcacc
     1501 gaaggtgtgc cggaaaagga cgccaaactg ctcggcgcgc atgccaggaa gttggggaag
     1561 gtcttcaacg ggccgtcttc catcggcatc atctcggcag gcgcctgtcg gctgggcgtc
     1621 atcggcgggg cattcgacaa cctggtgctc tcgaagctct accgtgaagg ttccttcggt
     1681 gtcgtcacta aatccggcgg tctctccaac gaaatcatct ggatctgctc gcagttcgcc
     1741 gatgggatca cgacggcgat cggaatcggc ggcgacgcct atcccggtac cgactatgtc
     1801 agctatctcg aaatgttcga gaacgatcct cagacaaagg ccgtcgtcat cgtcggtgag
     1861 atgggcgggg acctcgaaga acgcgcggcc gagtggtacg gcgccaagaa gcgacgaatc
     1921 aagttgatcg gtgttgtgtc cggattctgc caggagagtc tgccgaaggg gatgaagttc
     1981 ggccatgccg gggcgaagga aggcatgaag ggtgagggtt cggcacggtc caagtcagat
     2041 gcactcaaga aggctggcgc gattgtgccg ccgacgttcg gcgcgcttgg tcctgctatc
     2101 aaggaagcgt atcaagaact gttgaagtcc ggtcaggtca aggaagtggt tgagccggtt
     2161 gtactgccga agctgccgaa gccggtggaa gaagcgatga aggcggacga agtcatggtg
     2221 gcgccattga tccgtacaac gatcagcgac gaccgcggcg acgagccctg ctacgacggc
     2281 tatccggctt ccgagctgat caacaagggc tacgaaattc cccatgtcat ggggttgctc
     2341 tgggacaagc gtctcatctc gaagcaggaa gccgagatca tcaagcggat catgatgctc
     2401 tccgccgacc atggtccttg cgtcagcggg gcctatgcga ccatcttagc ggcttgcgcc
     2461 ggtatcgggt tgtcacagtc ggtggcagca ggcttaatta tgatcggtcc acgcttcggc
     2521 ggcgccgtca cggatgccgg tcgatacttc aagcatgcgg tcgacaatag gatgtccgtc
     2581 gatgagttcc tggcctacat gaagaagagc gtcggcccgg tgccggggat cggtcatcgc
     2641 gtcaagagcc tgcgcaatcc ggacaagcgg
//
LOCUS       DATDNW010000128         6908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_81127,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000128 DATDNW010000000
VERSION     DATDNW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81127"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VI359_03220"
     CDS             complement(<1..219)
                     /locus_tag="VI359_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY6973296.1"
                     /translation="MSRQLSDEERNMENVLRPQSLAEYVGQERMKESLRICIEAAKQR
                     QEALDHAIFYGPPGLGKTTIAHIIAREMG"
     gene            complement(246..740)
                     /locus_tag="VI359_03225"
     CDS             complement(246..740)
                     /locus_tag="VI359_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973297.1"
                     /translation="MHVIQSHLILRVAMGLVLSVGPGLWAQVGAEEPEPPKTVHKTCG
                     KCPDGFALTGVTEAPGICKDGDPTLVQCVPLGANMLPVCGSCPTGYVEIGSSSVPARC
                     GKQEGGRLSQCQLQKMELNLPDPTQGGKVCPPDCGSTQTPGQGALPPPPRFLPTPEKQ
                     ESQR"
     gene            complement(750..1361)
                     /gene="ruvA"
                     /locus_tag="VI359_03230"
     CDS             complement(750..1361)
                     /gene="ruvA"
                     /locus_tag="VI359_03230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY6973298.1"
                     /translation="MIALLTGRLAFKAPTHLALDVQGVGYEVFIPLSTYYGLPNLNET
                     ASLSIHTHVREDAIQLFGFLTQQEKDAFVLLTSVSGIGPKLALSVLSSLSVTDVVSAI
                     RTADLEKLATVPGIGKKSAGRIALELKDKLAKLDPSPTVSTRFTGGEKQDTTFDDALS
                     ALVNLGYRPQDAKGALQQVKKMKPDSMVLKDLIRDSLKELARG"
     gene            complement(1415..2179)
                     /locus_tag="VI359_03235"
     CDS             complement(1415..2179)
                     /locus_tag="VI359_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY6973299.1"
                     /translation="MGGHSHWATIKRHKSSQDAKRGKIFTRIIRELTIAARGGGDPDG
                     NPRLRLAIAKAKEANMPGDTMKKAIQRGTGELPGVTYEEFTLEGYGPGGTGLLLEITS
                     DNRNRTVAEIRSLFTKNHGNMAEAGAVAWQFQKKGLLTIEKGKVDEDSLLSLALDAGA
                     EDVKSSGKSFEIVTDAHDFEGVKKALTDAKIEIALAEITFIPQNTIRLEEKAAEQMLK
                     LMETLDEHDDVQKVHANFDISDEVMEKVAAAAAAAG"
     gene            complement(2318..3085)
                     /locus_tag="VI359_03240"
     CDS             complement(2318..3085)
                     /locus_tag="VI359_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973300.1"
                     /translation="MAAGSWRTIVGMAVILGAVLWWTVPVFAQHGDEEHHAVHETSTS
                     GHASHTVSPGALAWEGSVEGVAYSEFNHHLAGILVMLMGLAELAQASRLPSLAWARVL
                     LPLSMLAAGSFLLIWSDHEAWPIGSLSFGQTYFGGDHEILQHKTFGVLSLAVGTVELL
                     RRYRRLTHLAWTVPLPLLATVGGVMLFGHSHGEHPSAQKIAAHHAMMGTLALAAGSSK
                     ILSGWFHSPSAATRVTWEWIWGGLVFALGILLLWYSE"
     gene            complement(3167..3925)
                     /locus_tag="VI359_03245"
     CDS             complement(3167..3925)
                     /locus_tag="VI359_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEY6973301.1"
                     /translation="MKFVCLNCETYMNFEKVEKPGEGSLGVFFGCPSCGAKFSMVTNP
                     GETQMVSSLGVKLGGRTAAAEPFEMTRGTLKDEALAGSGQMAAYLNEKIHGGQPVAAS
                     AAPPSGKEGEKTGGGCPFSAMVAEMGLTTGGKAASGTTGALEFTWSSDAKEKLDRLPA
                     FVKPMVQSSVEAYARKQGYKTITLQVMDDSKNDSSSNGVTWSREAEQRLENIPDFIRP
                     MARKEIERVAKERGVATITAQVMDEAKDKFMKFM"
     gene            complement(3951..4871)
                     /locus_tag="VI359_03250"
     CDS             complement(3951..4871)
                     /locus_tag="VI359_03250"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY6973302.1"
                     /translation="MAPDKDLKTILGKLRYSDDAKVVQQITEQTKNVHARMAGIKHKL
                     VVMSGKGGVGKSMTTVNLALAFARQGAKVGLLDVDLNGPCVPRMLGLHGQSLQMTPEG
                     AIPPIGPLGIKVASMDFFLDEASPVRWKGPMDVSPVWLGLMEMNVIREFLADVIWGEL
                     DVLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIKVL
                     GIVENMSEYRCPSCGEQNELFEGNTEAMCEVLDLPLLGRIPFDRKLARTFDKGAPLLD
                     ETYPTIQRYQEIAGRIRTLLDYKKVLADKL"
     gene            complement(5037..6881)
                     /locus_tag="VI359_03255"
     CDS             complement(5037..6881)
                     /locus_tag="VI359_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6973303.1"
                     /translation="MKNTEAKPSENLNIVIVGHVDHGKSTLLGRLYADTGSLPDGKLE
                     KVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFTWKGRQYIIIDAPGHKEFL
                     KNMISGAARAEAALLLIDALEGVKEQSKKHGYLLSLLGVRQFAVVVNKMDLVGYRQDV
                     FEGIEKEYREFLSQFKAVPERIIPVSAKLGDNIVSRSENMPWYSGPTVLDMLSLFQKE
                     RARSEQPLRLPVQDVYKFDARRIIAGRIVAGRLKVGDQLVFSPSNKRANIKSVEAFNV
                     EPVPTEAHAGQSVGIALDEQIFVERGEIASHQGELPLVSTAFRANLFWLGRRPLEQGR
                     KYILRVATKEVDCEVATIHRIIDTMDLNQQQGGGSVGRNQVAELTLRTKAPVAFDLSS
                     SFEATGRFVLVDEYDIAGGGIVTEMVRDDQEFLREEARRRDFAWVKGEVGVEERAQQF
                     GHRAAIVLVTGGRHTGKSFLAKKLEARLVADGRHAYLLDGENLRRGLDADLSEAERSQ
                     TTEMARRYGEVARLLIDTGLIVVSTTNPFGLAYSEAAQAIRTLVHPAPVIAVHMSKTP
                     EEPPPGTDIALSGPTDFDSATRQILDELKRRGVLAQAIGAKPTFQYSI"
BASE COUNT         1475 a         2099 c         1764 g         1570 t
ORIGIN      
        1 gcccatttcc ctggcaatga tgtgagcgat agtcgtcttg cccagaccgg gcgggccgta
       61 gaaaatcgcg tgatcgagtg cttcctgtcg ttgtttcgcc gcttcgatac aaatacgaag
      121 ggactctttc atccgttcct gaccgacata ctccgccaaa ctctgcggtc ggagcacgtt
      181 ctccatattc cgctcttcgt cgcttaactg tctactcacc gtacgatcag ccatcagtta
      241 ttgcttcacc gttgtgattc ttgtttttca ggagtcggaa gaaatcttgg gggaggcgga
      301 agtgcgccct gccccggtgt ctgcgtactg ccgcaatcag gcggacagac ttttcctcct
      361 tgcgtcggat ccggcagatt cagttccatt ttctggagtt ggcactggga cagccgtcct
      421 ccctcttgct tcccgcagcg agccggcacg gaagaactgc caatttcgac atagcccgtt
      481 gggcaagacc cgcacaccgg caacatattc gcaccgagcg gtacacactg aaccaaggtc
      541 gggtctccat ccttgcaaat tcccggtgct tcggtgaccc ccgtcaaggc aaacccatca
      601 gggcatttcc cgcaggtctt atgcacggtt ttaggaggtt cgggttcctc cgcccctacc
      661 tgcgcccaaa ggccgggacc gacactgagc accaacccca ttgcgactcg aaggataaga
      721 tggctttgta tgacatgcat ggtcatgcgc taccccctgg ctagctcttt caagctgtca
      781 cgtatcaaat ctttcaagac catcgaatct ggtttcatct tcttcacctg ctgcagagcg
      841 cctttcgcat cctgcggccg atagccgagg ttgaccaggg cggagagcgc atcgtcgaat
      901 gtcgtatcct gtttttctcc tcccgtgaac cgagtcgaga ccgtcggact tggatccagc
      961 ttggcgagtt tatctttcag ttcgagcgcg attcgaccgg ccgatttctt tccgatgccg
     1021 ggcaccgtcg cgagtttctc gagatccgct gtacgaatgg cggacaccac atcggtgaca
     1081 gagagagaag acagcacact cagcgccaat ttcggtccga tcccggagac cgaggtcaaa
     1141 agcacgaatg cgtccttttc ctgctgcgtc aaaaacccga ataactgaat cgcgtcttcg
     1201 cgaacgtggg tatggatgct cagcgaggcc gtctcgttga ggttgggaag tccgtagtag
     1261 gtactgagag gaataaacac ttcatacccg actccctgaa cgtcgagagc cagatgcgtc
     1321 ggcgccttga aggccaggcg gcctgtcaat aaggcgatca tgtggccggt gtcgtaggac
     1381 tcacgctgtt accgagcgag gctgccgccc gttgtcatcc cgccgccgcc gcagccgccg
     1441 ccaccttttc catgacctca tccgaaatat cgaaattcgc atggaccttc tggacatcgt
     1501 catgttcgtc cagggtttcc atcagcttca acatctgctc cgccgctttc tcctcaagcc
     1561 ggatggtgtt ctgtggaatg aacgtgattt cagccagagc gatttcgatc tttgcgtcag
     1621 tcaaggcttt cttcacacct tcaaaatcat gcgcgtcggt gacgatttcg aaactctttc
     1681 cgctggattt gacatcttcc gccccggcat ccagggcgag cgacagcaga gagtcttcat
     1741 cgactttgcc cttctcaatg gtgagcagtc ctttcttctg aaactgccac gccactgccc
     1801 cggcctccgc catattgcca tgattcttcg tgaacaagct ccgaatctct gcgaccgtgc
     1861 gatttcggtt gtcgctcgtg atctcaagga gcagcccggt cccgcctggg ccataaccct
     1921 caagcgtaaa ttcttcatag gtcacaccgg gcaattcccc ggtgccgcgc tgaatcgctt
     1981 ttttcatcgt atcgccgggc atgttggctt ctttggcttt ggcaatggcc aagcgaagac
     2041 ggggattgcc gtccggatcg cccccgccgc gcgccgcaat ggtcagctca cggatgatgc
     2101 gcgtgaagat cttgccgcgc ttggcatcct gcgaggattt atgccgtttg attgttgccc
     2161 agtgactatg gccgcccatg agaacctcct cgtggctcgc gttgttccgc ttgccacgac
     2221 catgctgctt tacttgtcaa atgtggaatt ttgtactcgt agcacagggc tctgaagggt
     2281 gtcaattgcc gatcgacagg agaaagagag gagaacgcta ttccgaatac cagagaagga
     2341 ggatccccag ggcgaagacg agccctcccc agatccattc ccatgtgaca cgcgtcgcag
     2401 cggagggaga atggaaccag cctgagagaa tttttgatga cccggctgcg agggctaagg
     2461 tgcccatcat cgcatggtga gccgcgatct tctgcgctga tgggtgctct ccgtgtgaat
     2521 gaccgaataa catcaccccc cctacggtcg ccagaagcgg caacgggact gtccacgcga
     2581 gatgggtgag ccgcctgtac cggcgcagga gttctacggt gcccaccgct aatgacagaa
     2641 ccccgaatgt cttatgctgg agaatctcgt gatccccgcc gaaataggtc tgaccgaaac
     2701 tgagcgaccc gatgggccac gcttcgtggt cgctccagat caggagaaag gagccggcgg
     2761 caagcatcga cagaggaagg agcaccctgg cccatgcgag ggagggcaac cgactcgcct
     2821 gagccagttc agccaagccc atcaacataa caagaatgcc agccaaatga tggttgaatt
     2881 ccgagtaggc aaccccttcc accgacccct cccaagccag agcgccggga ctcactgtat
     2941 gggaagcatg gcctgaggtc gacgtttcat ggacggcatg atgttcctca tcgccatgtt
     3001 gagcaaagac aggaacggtc caccaaagaa cagcacctaa gattactgcc ataccgacta
     3061 tggtccgcca actgccagca gccatgcttt tctaagctcc ttcaatagaa aggcccatcc
     3121 gattcatcgg atgggccttt gtccccccgc aacgaacatc acagatttac ataaatttca
     3181 tgaacttgtc tttcgcttca tccatcacct gtgcggtgat ggtagcgacg ccacgctcct
     3241 ttgcaactcg ttcaatttct ttccttgcca taggacggat gaaatccgga atattctcca
     3301 gccgctgctc cgcttcgcgc gaccaagtca caccgttcga cgacgaatca ttcttggaat
     3361 catccatcac ttggagggtg atcgtcttat acccctgctt gcgagcatac gcttccacac
     3421 tgctctgcac catcggcttc acaaatgccg gaagccgatc tagcttttcc tttgcatccg
     3481 agctccacgt gaattctagt gctcccgtcg taccgctggc agcctttcct cccgtcgtga
     3541 gccccatctc ggcaaccatg gcagaaaagg gacagccacc gccggtcttt tccccttcct
     3601 taccggacgg gggcgccgca gaagcggcta ccggttgccc accatgaatc ttttcattca
     3661 aataggccgc catttgcccg gacccggcga gcgcttcgtc cttcagcgtt ccgcgcgtca
     3721 tttcaaacgg ttctgccgct gcagttcgtc cacccagttt cactcccaac gaactgacca
     3781 tttgagtttc acccgggttg gtgaccatgg agaacttcgc tccgcaagaa gggcacccaa
     3841 agaagacacc gagcgatcct tccccgggct tctcaacctt ctcgaagttc atataggttt
     3901 cgcagttaag acaaacgaat ttcattgtcg ctcctcccgc ctcgatcccg tcacagctta
     3961 tccgccagga ctttcttgta gtccaacagg gtgcggatac gtccggcaat ttcctgatat
     4021 cgctgaatgg tcgggtatgt ttcgtcgagc aagggggctc ccttatcaaa tgtgcgggcc
     4081 aacttccggt caaacgggat gcgtccaagg agcggaaggt ccaagacctc gcacatggct
     4141 tctgtattgc cctcaaacaa ctcattctgc tccccgcagg acgggcaacg gtactcactc
     4201 atattttcca cgattcccaa caccttgata cccatatcac gtgcatacgt cacggacttt
     4261 tgaacgacat ccgaagccac ttccgacggt gtcgtgacga cgatggcgcc ggccaagtca
     4321 ggaatgaagc ccgcgatcac gggcggcttg tcggcagctg cgccaggagg caggtcggcg
     4381 agtagcacat ccaattctcc ccagatcacg tcggccaaga actctcggat gacattcatc
     4441 tccatgagtc cgagccagac cgggctcaca tccatcggac ccttccaacg aaccggcgat
     4501 gcttcatcga ggaagaaatc cattgaggcg acttttatac cgagcggacc gattggaggg
     4561 atggctcctt caggagtcat ctggagagac tgaccgtgga gacccagcat acgcggaaca
     4621 cagggaccgt tcagatcgac gtcaaggagc cccaccttag ccccctgacg ggcaaaggcc
     4681 agcgccagat tgacggtcgt catacttttg ccgactccgc ccttgccgct catcacgacc
     4741 agcttgtgct tgatgccggc catccgggcg tgaacgttct tcgtctgctc ggtgatctgc
     4801 tggaccacct tcgcatcatc tgaataccgc aatttcccga gaatcgtctt caggtctttg
     4861 tcaggggcca tgagttctaa gtctctccaa aacaagtggt ttctaagagg aggtacggta
     4921 acacacggat ttccggagag tcaaaccatc agggagcgga gccttggtcg tgatctcccg
     4981 actcagatgt tcctttcgtt ccatactgaa accgagcggc cgagatgccg tgcttgctag
     5041 atcgaatact gaaacgtcgg ttttgctccg atggcctgtg ccaacacacc tcgacgcttg
     5101 agttcatcca aaatctgacg cgtcgccgaa tcgaagtcgg tcggaccgga cagagcgata
     5161 tcggtaccag gaggaggctc ctctggtgtc ttgctcatgt gcaccgcaat gaccggcgcc
     5221 gggtgaacga gggtacgaat ggcttgcgcc gcctccgagt aggccagacc gaagggattc
     5281 gtggttgata ctacgattag ccccgtatcg attaagagcc ttgccacttc gccgtaccgc
     5341 cgagccattt ccgtagtctg gcttcgctcc gcttcagaca agtcggcatc caacccgcgc
     5401 cggaggtttt ctccatccag aaggtaggca tggcgaccat ccgcgacgag cctggcctct
     5461 aacttttttg ctaggaacga cttcccggta tggcgacctc ctgtgacgag cacgatcgcg
     5521 gcccgatggc cgaattgctg cgctcgttcc tcgaccccca cctcgccctt aacccacgcg
     5581 aaatcccggc ggcgagcttc ttcccgcaga aactcctgat cgtcgcggac catttccgtg
     5641 acgattccgc caccggcaat atcgtactca tcgaccaaca caaaccgacc agtggcttca
     5701 aacgaggacg agagatcaaa cgccacagga gccttggttc tgagcgttaa ctcagccact
     5761 tgattcctgc cgactgaccc tccgccctgc tgctgattca agtccattgt atcgattatg
     5821 cgatggatcg tcgccacttc gcagtcgact tctttggtgg cgacccgcag gatatatttt
     5881 cgcccttgtt ccaacggccg gcgccccaac caaaacaggt tggcgcgaaa tgccgttgag
     5941 acgagtggca actcaccctg gtgtgaggcg atctcacctc gctccacaaa gatctgctca
     6001 tcgagcgcaa tgccgactga ctgtcccgca tgggcctcgg taggaacagg ctcgacatta
     6061 aaggcctcaa cggatttaat gttcgcgcgt ttgttcgatg gagaaaagac aagttgatcg
     6121 ccaaccttca accgccctgc cacaatccga ccggcgatga tccgacgagc atcgaattta
     6181 tagacatctt ggacgggcag gcgcagcggc tgctcggacc gagccctctc tttctggaac
     6241 agactcagca tatctaacac tgtcggtccg ctgtaccaag gcatgttctc actgcgactg
     6301 acaatgttgt cacccagctt ggcactcaca ggaataatcc gctcgggcac ggctttgaat
     6361 tgactgagaa actcccgata ttccttttca ataccctcga agacatcctg tcgataaccg
     6421 acaaggtcca ttttgttgac gacgacggca aactgccgga ctcccaataa agaaagcagg
     6481 tacccgtgtt tcttggattg ctccttgact ccttccaagg catcgatgag aagcagcgcc
     6541 gcttcagcac gcgctgcgcc ggagatcatg ttcttgagaa actctttgtg ccctggcgcg
     6601 tcgataatga tgtactgccg acccttccac gtgaagaatg ttctcgccgt atcgatcgtg
     6661 atgccttgct cctgctcctc caagaacgca tcgaacagga atgcgtactc gaattccttt
     6721 ccctgttgcc ggcagatagc ctgaactttt tccagtttgc cgtcgggcaa cgaccccgtg
     6781 tcggcgtaga gacgacccaa caaagtggac ttgccatgat caacatggcc gacgatgacg
     6841 atattcaagt tctcggatgg tttggcttct gtattcttca tctcaattca tgtccggttg
     6901 ttgaagat
//
LOCUS       DATDNW010000129         3996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_81541,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000129 DATDNW010000000
VERSION     DATDNW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81541"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VI359_03260"
     CDS             <1..753
                     /locus_tag="VI359_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973304.1"
                     /translation="QFQLYMQSGKQGEAEQWLGRLVNEKPTDAEVRELLGSLFERKGD
                     GPGAALHYARALELLLEHPDDKKPERPGALYNKVKELAPASPLVAKLAPMFAPTSEEP
                     AAVSAQADDSTPVANGFDPNIHYTLGVAFKNMGLLDEAKEEFVQSMAGNDLFLDSCLM
                     TAVCLKEQGQGKAASEQLEQLINDARCQGAKAQAIRYELGLLYEAQEQWQKAAAIFES
                     IPTFHDVPKRLDALRGAQKPKPAAAGAFRYAK"
     gene            complement(758..1717)
                     /locus_tag="VI359_03265"
     CDS             complement(758..1717)
                     /locus_tag="VI359_03265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY6973305.1"
                     /translation="MSTIRPLLYVSRLLPHPVMATIQERFRLVQAPTEASPTPAALRH
                     GFSRADAAICTLTDTVDDDLLSSAAHLKILANYAVGYNNVDLDAARRHHIVVTNTPDV
                     LTDATADLTWALLLATARRVVEGDALLRSSRWTGWTPTQLLGREVSGKTLGIIGMGRI
                     GQAVARRAEGFRMGVRYHSHRPIPAPPGIWESCPLDELLQESDFVTLHVPLNSETRHL
                     IGARELSLMRPTAILINTSRGSVIDEAALAHALQSGTIAGAGLDVYEEEPAVHPLLPS
                     LKQVVLLPHLGSATMQTRVQMGLICLENIEAVLEGRPAPNRVA"
     gene            1810..>3996
                     /locus_tag="VI359_03270"
     CDS             1810..>3996
                     /locus_tag="VI359_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6973306.1"
                     /translation="MTLILPDASRAVARDLRRLLGSDLVQDDGPTKTAYAVDASIYRM
                     EPQAVVLVESERDIAATVTYAVQRGISLTPRAAGTNLTGSAIGSGIILDVSRLNRILE
                     LNRDERWARVQPGIVLAELNKQLGRDGLLFGPDPSSGDMCKLGGMVANNSAGPHTIRY
                     GAVKDNVQALRVCLSSGEWLNARTYGLDDPQLSGLLDRHAPLKGLLSLVRNQATVIAN
                     KQPTVSKNSSGYNLFGLADGLTRRQFDLPKLFVGSEGTLGVVSEATLRLVNKPTATVT
                     ALIHFLRLEDVGEAVPKLLELKPSALEVMDANTLDLIGRSKHGIPPDAAATLLTELDS
                     DKGDDDLRDQATRMAAICRQYRLASDLVLAYDQDRREQLWKARKSLYPTLYRFDARKK
                     PINFVDDVVVSADRISELIRYLEEFFQGQQVPVAIFGHIGNGNAHIVPLLDVNDRQDF
                     EKMVQGYHEIHQTVLDRFGGSICGEHGDGRVRAEYVRKMFGPELYGMFEQVKRLFDPS
                     GVLNPGVKISDRPFTDHIDYTRLSKSCATCAKCNSVCPVYDVFQSEDMSSRGWYEIVT
                     AKDYSYLNSKRVVEACLNCKSCRTICPAGVDVSELILQKRAEQPNYIAGLIFRLHANQ
                     AVFERILKLFAKSQRIWDRPSIRRIIERLTRPLLRGIAQTAKLPFDMVLPRLARRHLR
                     DRHADLVTMEGARHSPVAYFHGCAANYFDDGVGDAVIEVLRKHGVTP"
BASE COUNT          882 a         1141 c         1151 g          822 t
ORIGIN      
        1 caattccaac tctatatgca gtctgggaaa caaggggagg cggagcagtg gctcggacga
       61 ctggtaaacg aaaaaccgac cgacgccgaa gtgcgtgaac tattggggtc cttgtttgaa
      121 cgtaaaggtg atggcccggg agccgcgctg cattatgccc gtgcgcttga attgctcttg
      181 gaacatcctg atgacaagaa accagaacga cctggcgcgc tctacaacaa agtcaaggaa
      241 ctggctccag ccagtcctct ggtggccaaa cttgccccga tgtttgcacc cacatcggaa
      301 gaaccggcag cggtgagcgc tcaagctgac gactcgactc cggtcgccaa tggattcgat
      361 ccaaacattc actacacgct gggtgtcgct ttcaagaata tggggttgct cgacgaggcc
      421 aaagaagaat ttgtccagtc gatggcagga aacgatctct tcctcgattc ttgtctgatg
      481 acggcagttt gcttgaaaga acaaggtcag ggaaaggccg caagcgagca gctcgaacaa
      541 ctgataaacg atgcgcgctg tcagggtgcc aaagcccagg ccattcgata cgagctggga
      601 ttgctttatg aggcgcagga gcaatggcag aaagccgctg ccatcttcga atcgattcct
      661 acttttcacg atgtgcccaa gcgtcttgac gcgctacgag gcgctcagaa accaaaaccc
      721 gccgctgctg gagccttccg ctacgccaaa taggaactca ggcaacccgg ttcggggccg
      781 gtcgaccttc cagtacagcc tcaatattct ccagacagat gagccccatc tggacccgcg
      841 tctgcatcgt ggcggagccg agatgcggca ggaggacgac ctgcttcaac gaaggaagca
      901 gcggatggac cgccggttcc tcctcataga catctaatcc ggcgccggcg atcgtgccag
      961 attgcagagc atgagcgagc gcggcctcgt caatgaccga cccgcgactc gtgttgatca
     1021 gaatcgctgt cgggcgcatg agcgagagct cacgagcacc aatcaagtga cgggtctcgg
     1081 aattcaacgg gacgtgcaac gtcacgaagt ccgactcctg taacaattcg tcaagaggac
     1141 atgattccca aatgcccggc ggcgccggga tcggccgatg actgtggtag cggacaccca
     1201 tgcgaaatcc ctcggctcgt cgagccactg cctggccgat gcgtcccatc ccgataatac
     1261 ccagcgtctt tcctgacact tccctgccta agagctgagt cggcgtccag ccggtccacc
     1321 ggctcgaacg aagtagcgca tcgccttcga cgactcgacg ggccgtagcc aacagaagtg
     1381 cccaggtcag gtcagctgtt gcatccgtca gaacatccgg cgtattggtg accacaatgt
     1441 ggtgtcgacg agcggcgtcg agatcaacat tgttgtagcc caccgcatag ttcgccagga
     1501 tcttcaagtg agcggcggat gacaacagat cgtcatccac cgtatcagtc agcgtacaga
     1561 tggcggcgtc cgcgcgggag aatccgtggc gcaacgcggc tggagtgggc gaggcttcag
     1621 tgggagcctg caccaaccga aaccgttcct gaatggtcgc cattacaggg tggggcaaga
     1681 gacgggatac atagagcagg ggacgaattg tcgacatggc gcgctcctgc tggccatctt
     1741 aatgtgtctg gaatcattgc tcaaccgatg ctggcggact ttttcaacat cctgctagaa
     1801 tgcgcagtca tgacattgat actccccgat gcttcccgcg cggtcgcgcg cgaccttcgt
     1861 cgtctccttg gttctgatct cgtgcaggac gatggtccga ccaagacagc ctatgcggtg
     1921 gatgcaagca tttatcgcat ggaaccccag gctgtcgtgc tcgttgagtc ggagcgtgac
     1981 atcgctgcca cagtgaccta tgcggtgcaa cgagggattt cactcacgcc ccgtgccgcc
     2041 ggtacgaacc tgaccggctc ggccatcggc tccggcatca ttctggatgt ctcacgtctc
     2101 aatcgaatct tagaactgaa tcgcgatgaa cgctgggccc gggtccaacc gggcatcgtg
     2161 ctggccgaac tcaataaaca attggggcgt gacggactgt tgtttggtcc cgatccttcc
     2221 agcggcgaca tgtgcaaact cggtgggatg gtcgcgaata attccgctgg acctcatacg
     2281 atccggtatg gagcggtcaa agacaatgta caggcccttc gggtgtgctt gtcttcaggg
     2341 gaatggctca acgcgcggac ctatgggttg gatgatccac agctcagcgg gctgctcgac
     2401 cgccatgcgc cgttgaaggg cctcctctca ctcgtccgga accaggcgac agtcattgca
     2461 aataagcagc cgaccgtcag caaaaatagt tccgggtaca acctcttcgg tttggccgat
     2521 gggttgaccc gcagacaatt cgacctcccc aagttgttcg tcggcagcga aggcacccta
     2581 ggcgtagtga gcgaagccac gctgcggctc gtcaacaagc caacggcgac cgtgactgcg
     2641 ctcatccact ttctacggtt ggaagatgta ggagaggccg tgcccaagtt gctcgagctg
     2701 aagcccagcg ctttggaagt gatggatgcg aacacgctcg atctgatcgg gcgatccaag
     2761 catggcattc ctccggatgc tgctgcgacc cttttgaccg aactcgacag cgacaagggg
     2821 gacgatgacc tgcgggatca ggcgacacgc atggcggcca tctgtcgcca atatcgactg
     2881 gcctctgatc ttgtgctcgc ctacgatcag gatcggcggg agcagctctg gaaggcccgc
     2941 aaatccctat acccgacctt gtaccgtttc gacgcaagaa agaagcccat caacttcgtc
     3001 gatgacgtcg tcgtctcggc agatcgaatc agcgagctga tccgctattt agaggagttt
     3061 tttcagggac agcaggtccc ggtcgctatc tttggtcata ttgggaatgg caatgcccac
     3121 attgtgccgc tgctcgacgt gaacgatcgc caggatttcg aaaaaatggt ccagggttac
     3181 catgaaatcc atcaaacggt cctggatcga ttcggcgggt ccatctgcgg cgagcacggc
     3241 gacggccgcg tgcgtgccga gtatgtccgc aagatgttcg gtcctgagct ctatgggatg
     3301 ttcgagcagg tgaagcgcct gttcgatccg tccggcgtcc tcaatcctgg cgttaaaatc
     3361 agcgatcgtc cgttcaccga ccacatcgac tacacgagac tctccaaatc gtgcgcaacc
     3421 tgtgcgaaat gtaactccgt ctgtcccgtc tacgacgtgt tccaatccga agacatgagt
     3481 tcgcgcgggt ggtatgagat cgtcacagcc aaagactaca gctatctaaa ctcgaagcgg
     3541 gtggtagagg cctgtttgaa ttgtaaatct tgccgcacga tctgtcctgc cggcgtcgat
     3601 gtctcggaac tgattcttca gaagcgggct gaacaaccga attacatcgc tggtctgatc
     3661 tttagattgc atgcaaatca agctgttttt gagagaatcc tgaagttatt cgcgaagtcg
     3721 caacggattt gggatcgacc tagcattcgt cgaatcatcg agcgactcac gcggccgctt
     3781 ctccgtggca tcgcgcagac tgccaagctg ccgttcgaca tggtcttgcc tcgtcttgcc
     3841 cggcgacacc tccgtgatcg ccatgcggac ttggtcacca tggaaggagc acgccactcg
     3901 ccggtggcct atttccatgg gtgcgcggcc aactacttcg acgacggggt aggagatgcg
     3961 gtgatcgagg tgctgcgtaa gcacggtgtc acgccg
//
LOCUS       DATDNW010000130         4088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_834286, whole genome shotgun sequence.
ACCESSION   DATDNW010000130 DATDNW010000000
VERSION     DATDNW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834286"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="miaA"
                     /locus_tag="VI359_03275"
     CDS             <1..967
                     /gene="miaA"
                     /locus_tag="VI359_03275"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY6973307.1"
                     /translation="RLSEAQVRLRPLVVIVGPTAVGKSRIALEIAKAFETDILTADSR
                     QVYRGMDIGTDKPTAGQLQDVTHRLIDLVEPNQPFNTGLYRRHAVQAIEDLYRARRLP
                     LVVGGTGLYVRTLLKGLCKAPPGDPVIRASLRREAQMHGRDHLYTQLVACDPATAARL
                     HPRDESKVLRALEVHRLSGRPISEFQQQHGFTERPFTSLMIGLNRAREVLYRRIEERI
                     DWQLDHGLVDETKRLLARGYSRDLPAMKGLGYRHVSCYVAGEYDAEEMARRFKRDTRR
                     FAKRQMTWFRKEPGIPWMMIEESEPLTVTTHRIVKHVDAFLADLG"
     gene            1024..1929
                     /gene="mtnP"
                     /locus_tag="VI359_03280"
     CDS             1024..1929
                     /gene="mtnP"
                     /locus_tag="VI359_03280"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY6973308.1"
                     /translation="MGTARRHVRARADVGIIGGSGLYEIEGLRNVTRVPVRTPFGAPS
                     DAIMLGELDRVRVAFLSRHGRGHRISPAEINYRANIYALKSLGVRRVISVSAVGSMKE
                     SIKPGDIVLPDQFIDLTKRRASTFFEGGIVAHVGFAEPICGALSASLFEGAQSIGASV
                     HRNGVYICIEGPQFSTKGESRLYRQWGVSVIGMTNMPEAKLAREAELCYATVALVTDY
                     DCWHETEEPVTVEAILATLRKNVVLAKQLLRISVKPVAAVETCGCQLALENAVVTAPE
                     RIPAGVRRKLGLLLDRIMSPTTRGR"
     gene            1933..2847
                     /locus_tag="VI359_03285"
     CDS             1933..2847
                     /locus_tag="VI359_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY6973309.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVAEVLGGSATYFSAAASFFTTVD
                     LIAVVGEDFPSQHVAFLKSRGIDLTGLERRSGATFRWQGEYTHQLNEAHTLDTKLNVF
                     ETFRPKIPDAYRSPDVLFLGNIDPELQLDVLQKLPRPPLVACDTMNFWINGKREALWR
                     VLEKVDILIVNDGEARALGGSPNLVKVAQLVLARGPKHLIIKRGEYGVLMFNEKQVFG
                     APAFPLEDVRDPTGAGDTFAGGFLGYLAATGNRTNEAIKQAIIFGSVMASFTVESFSL
                     DRLRILDYKEIHERFRAFKRLTHFEDIA"
     gene            2844..3530
                     /locus_tag="VI359_03290"
     CDS             2844..3530
                     /locus_tag="VI359_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973310.1"
                     /translation="MTHSSLVGTMIRSWSYGLVVVLPLVGIVQGCATPEETVKKSQGY
                     YQEGIATLDSDKQKSYVSFQKALQVDPQNKEAHYGLGHILAGQGKWALAEEHFRAALS
                     IDQDYSEAHTYLGQVLEAQNRWPEAVQEFRLALTNPLYATPDLARFHLGRALAHEGDY
                     HGAMEALEDALVVDPPHVSPARTNLELGRVYYKLGFERRAKETLTRVTTLDKNGEYGA
                     AAKELLAKLK"
     gene            3544..>4088
                     /locus_tag="VI359_03295"
     CDS             3544..>4088
                     /locus_tag="VI359_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6973311.1"
                     /translation="MESIGEFFKQVRETKGLTVDEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFTQSAGAFYEKQDERERLRVRQAEEERKRQA
                     NRKAVAVAIGIAILTLVFLLSREQSSVFVRRSASDLPAPAKRAAPAPPEPVAHQEPPT
                     SLPGAKANAPGATSSKPATESA"
BASE COUNT          870 a         1082 c         1263 g          873 t
ORIGIN      
        1 gcggttgtcg gaggcacaag tccggctgag gcccttagtc gtcatcgtgg ggccgacggc
       61 tgtcggtaag agccgcatcg cactggagat cgcgaaggcg ttcgagaccg acattctgac
      121 cgcggactcc cggcaggtct accgaggaat ggatatcggg accgataagc cgacggcagg
      181 tcagctgcag gacgtcactc atcgcttgat cgatctggtc gaaccgaacc aaccattcaa
      241 cacaggtctc tatcgacggc acgccgtcca ggccatcgaa gatctgtatc gtgcccgccg
      301 tctcccactg gtcgtcggcg gcacaggcct gtatgtacgg acattgctca aaggcttgtg
      361 caaggcccct ccgggagatc cggtcattcg ggcatcgttg cggcgcgaag cgcagatgca
      421 cggacgagat catctctaca cgcagcttgt tgcctgcgat cctgcgaccg cggcccgatt
      481 gcatccacgc gacgagtcga aagtgttgcg cgccttggaa gtgcatcggt tgtccgggcg
      541 tccgatctcg gagttccaac agcaacatgg gtttaccgag cggccgttta cgtccttgat
      601 gatcggacta aaccgcgcgc gcgaggtgct ctatcgacgt atcgaagaac ggatcgattg
      661 gcaattggac cacgggttag tggatgaaac gaagcggttg cttgcccgtg gttatagtcg
      721 agatctcccc gccatgaagg gcctgggtta tcgccacgtg tcctgctatg ttgcgggtga
      781 gtacgatgcc gaggagatgg cccgccggtt taagcgggac accaggcggt ttgccaagcg
      841 acagatgacg tggtttcgca aagaaccggg catcccctgg atgatgattg aggagtcgga
      901 gccgctcact gtcacgacgc accgtatcgt caaacacgtt gatgcatttc ttgccgacct
      961 tggatagcgc atgttgtgtc cggtaagttg gaccggaagg gagcatgata caggaggact
     1021 acagtgggaa cggctcggcg gcatgtgaga gctcgcgcag atgtcggcat catcggcgga
     1081 agcgggttgt atgagatcga aggacttcgg aatgtcacgc gcgtgccagt tcgcacgccg
     1141 ttcggagctc cgtccgacgc aatcatgctg ggtgaactgg accgagttcg tgtcgctttt
     1201 ctctcgcgcc atggtcgcgg acatcgaatc agtccagcgg aaatcaatta ccgcgcgaat
     1261 atctatgctt tgaaatctct cggagtccgg cgagtcattt ccgtcagcgc cgtcggcagc
     1321 atgaaggaat cgatcaagcc gggtgacatt gtgctcccgg accagttcat cgatctcacg
     1381 aaacgacggg cttccacctt cttcgagggt ggcatcgtcg cccatgtggg attcgccgag
     1441 ccgatttgcg gggctttgag tgcctcgctc tttgagggag cgcagtcgat cggtgcatcg
     1501 gttcatcgga acggagttta tatctgcatc gaagggcctc agttctcaac gaagggagag
     1561 tctcggctgt atcgtcaatg gggcgtcagc gtgattggta tgacgaatat gcccgaagcg
     1621 aaactcgctc gtgaagcgga actctgttat gccacagtcg cgctggtgac cgattatgat
     1681 tgctggcatg agacggaaga gccggtcaca gtcgaagcca ttctcgccac gttgcgaaag
     1741 aatgtggtgt tggcgaagca actccttcgg atcagtgtaa agccggttgc cgctgtcgag
     1801 acctgtggct gccagcttgc gttagagaat gccgtggtca cagcccccga gcggataccg
     1861 gcgggagtcc gtcgtaaact ggggttgtta ctcgatcgaa tcatgtctcc aacaacaaga
     1921 ggtcgatgag cgatgggaaa attgttagtg gtcggctccg tagcgctgga taccgtcaag
     1981 actccattcg gggaggttgc cgaggtactg ggtggttcgg ctacctactt ctcggcggcg
     2041 gccagcttct ttacgaccgt tgatctaatc gcggtggtcg gagaggattt tccgtctcag
     2101 catgtcgcgt tcttgaaaag ccgcggtatt gatctgaccg gtctcgagcg acgatccggc
     2161 gcgacgtttc gatggcaggg cgaatatacg caccaattga acgaagccca cacactcgac
     2221 acgaagctca atgtcttcga aacgttccgt cccaagatcc ccgatgccta ccgatcgccg
     2281 gacgtgttgt tcttggggaa catcgacccg gaacttcaat tggatgtgct gcagaagctt
     2341 ccccgtccgc cgttggtggc ctgcgacacg atgaatttct ggatcaacgg gaagcgagag
     2401 gcgttgtggc gcgtgctgga gaaagtcgac attctcatcg tcaatgatgg agaggcgcgc
     2461 gccctcggcg gaagtcctaa tctggtcaaa gtggcgcagc tggtgctggc gcgaggtccg
     2521 aaacatctca tcatcaagcg gggtgagtat ggagtcttga tgtttaatga gaagcaggtc
     2581 tttggcgcac cagcctttcc tctcgaagat gttcgggatc cgaccggtgc tggcgatacc
     2641 ttcgccggcg ggtttctcgg gtatctggca gcgacgggaa atcggacaaa cgaggcgata
     2701 aagcaagcca tcatcttcgg cagcgtcatg gcgtcgttta ccgtagaatc ctttagtctt
     2761 gaccgtttgc gaatcctgga ttacaaagag attcacgaac gcttccgagc gttcaagcgg
     2821 ttaacccatt ttgaggacat tgcgtgacgc actcctctct cgtcggaacg atgattcggt
     2881 cgtggtcgta tggtctcgtc gtggtgcttc cactcgtcgg gattgtgcaa ggctgtgcga
     2941 cacccgagga gacggtaaag aaatctcaag gctattacca agagggtatt gcgacgctgg
     3001 attcggacaa gcagaaatcc tatgtgtcgt tccagaaagc gctccaagtc gatccgcaga
     3061 ataaggaagc ccactacgga ctcggccata tcctcgccgg acaggggaaa tgggcactgg
     3121 cggaggagca tttccgcgcc gctctgagca tcgaccagga ctattccgag gcgcatactt
     3181 atctggggca ggtgctcgaa gctcagaatc gctggcctga agctgttcag gagtttcgcc
     3241 tcgcattgac gaatccgctc tatgcgacgc ctgatctagc gcgctttcac ctcggccgcg
     3301 cgctagccca tgagggggac tatcacggtg ctatggaagc gttggaagat gctctcgtgg
     3361 tcgaccctcc tcatgtgtca cccgcccgca ccaatttaga gttgggcagg gtgtattata
     3421 agctgggctt tgagcggcgg gccaaggaaa ctctgacgag agtgacgacg ctggataaga
     3481 acggtgagta cggagcggcc gcgaaagaat tgttggcgaa attgaagtaa gagaatattc
     3541 gacatggaat caatcggaga attcttcaaa caggtgcgcg aaaccaaggg gctgacggtc
     3601 gacgaagtcg cctcgaagac ccgcatccgc acggatttcg tcaaggcgct cgaagaaggc
     3661 aactttgcca aactgcccga tcaggtgttt gcaagagggt tcgtgcggtc ctacgcgcga
     3721 tcgttgggac tagatgaaga agatgccatc catcggttta cgcagtcggc gggggccttc
     3781 tacgagaagc aagatgagcg ggaacgtttg agggttcggc aggccgagga agagcgaaaa
     3841 cggcaagcca atcgaaaagc tgtcgctgtg gcgatcggca ttgccattct cacgttggtg
     3901 tttctgctga gtcgtgaaca gtcctcggtc ttcgtccgca ggtccgcgtc ggatctgccg
     3961 gcaccggcca aacgggccgc accggcacct ccagaacctg ttgcacatca ggagccgcca
     4021 acgtctttgc ccggagcaaa ggcgaatgct cctggtgcga cctcgtccaa accggcaact
     4081 gagagtgc
//
LOCUS       DATDNW010000131         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1242318, whole genome shotgun sequence.
ACCESSION   DATDNW010000131 DATDNW010000000
VERSION     DATDNW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242318"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VI359_03300"
     CDS             complement(<1..978)
                     /locus_tag="VI359_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY6973312.1"
                     /translation="MAMQEFIAKIGKGPKAAKDLTWDEAKQVMKALIEGDATQAQVGA
                     FLLAMRIKMESVTELASFTATARSYVAPVPVPRELEVVDVPTYAGKQETFHALAGAAI
                     VAAAGGATILMHGYDGVPGRLGTAGILKGLGVPIDLEPKSIGGELAKKGLAYLDIGLY
                     HPPIYRFLEMRRELGVRNVFHPIARLLNPARATSQIVGLSHPPYFEKTAEVLRMLGCP
                     RALVIRGVEGDPELSLASVTKVLELRDERITPLTLAPKDVGLTMGASREMAGFPPDQQ
                     EKEEELLRRILQNQIQGGPREWVLLNAAVLLYAAGKGTSMAACVPIARRV"
     gene            complement(981..1700)
                     /locus_tag="VI359_03305"
     CDS             complement(981..1700)
                     /locus_tag="VI359_03305"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY6973313.1"
                     /translation="MTKLSPEEVKAWGESFAAKQPQEVLAAAIERYAPEIVLACSFGA
                     EDVVLVDMIHRINPTVPLFYLDTDFLFPETYAVRDRVIERYGLKPEQIIQMKSLLTPE
                     AQAQQYGERLWAQEPREKGPDHCCNLRKIEPLTRILKQYAAWITGIRRDQSPTRANAG
                     LIEWDDKFQLVKVNPLAKWTWDDVWTYIRLYEVPYNELHDRNYPSIGCTHCTAPVMPG
                     EDLRAGRWRNFGKTECGLHRT"
     gene            complement(1861..>2187)
                     /locus_tag="VI359_03310"
     CDS             complement(1861..>2187)
                     /locus_tag="VI359_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY6973314.1"
                     /translation="LAGEGEASPVPKPVVLPVKDIAAVVSAPTSKGAPVYDLRGVACP
                     LNYVKTKLKLEMMDAGEQLEVWLDAGEPIKNVPMSLKNDGHKVLLQEALEPEAAHYRV
                     LVEKVE"
BASE COUNT          431 a          696 c          570 g          490 t
ORIGIN      
        1 cacccgtcga gcgatcggaa cgcaagccgc catcgacgtg cctttgccgg ctgcatacag
       61 caacaccgcg gcattgagta acacccactc ccgggggccg ccctggattt gattctggag
      121 gattcgcctg agcaattctt cttccttctc ctgctgatcc ggtggaaagc cagccatttc
      181 acgcgacgcg cccatcgtca accccacatc cttcggggcc aacgtcagcg gggtaatacg
      241 ttcgtcccgg agttctaaga cctttgtcac cgaggccagc gacaactcgg gatcgccttc
      301 gaccccccga atcacgaggg cgcggggaca gcccagcatc ctcaatactt cggcagtttt
      361 ctcaaaatag ggtgggtggg agagtcccac aatttgcgac gtcgctctcg ccggattaag
      421 caatcgtgca attggatgaa agacgttcct gacgccgagc tctcgtcgca tttccagaaa
      481 tcggtagatg ggcgggtgat agagtccaat gtcgagatag gcaagccctt tcttcgcgag
      541 ctcgcctccg atggatttcg gctcaagatc gattggaacg cccaacccct tgaggattcc
      601 agcggtcccg agccgtccag gaaccccgtc gtatccatgc atcagaatcg tcgccccgcc
      661 tgcggctgct acaatcgcgg ctcccgcgag agcatggaag gtctcctgtt tccccgcata
      721 ggtggggaca tcaaccacct ccaattctcg cggaaccgga accggcgcca cgtaggagcg
      781 ggccgtggcg gtgaacgacg ccaactccgt gaccgattcc atcttgatcc gcatcgccaa
      841 cagaaaggcg ccgacttgcg cctgcgtcgc atcgccctca atgagggcct tcatgacctg
      901 cttggcctcg tcccaggtaa gatctttggc cgccttgggc cctttcccga ttttcgcgat
      961 gaactcctgc atggccatcg ttacgtcctg tgcaagccgc actcggtctt cccgaagttt
     1021 cgccagcgtc ccgcgcggag atcttctccc ggcatgacgg gagccgtaca atgagtgcag
     1081 ccgatgctgg ggtaattgcg atcatgtaat tcgttgtacg gtacttcgta cagacggatg
     1141 taggtccaga cgtcgtccca cgtccacttc gcaagtggat tgactttgac aagctgaaac
     1201 ttgtcgtccc attcgatcaa tcccgcgttg gctcttgtcg gtgactggtc tcgtctgatg
     1261 ccggtgatcc aggctgcgta ctgcttcaat atacgggtca agggttcgat ctttcgtagg
     1321 ttgcagcaat gatccggccc tttttctcga ggctcttgag cccacaagcg ctcaccatat
     1381 tgttgcgcct gggcttccgg agtgaggagc gacttcatct gaatgatctg ttccggcttc
     1441 aatccatacc gctcaatgac tcggtcacgg actgcgtagg tttcgggaaa cagaaaatcc
     1501 gtatccagat aaaacagtgg gacggtcgga ttgatacgat ggatcatgtc caccaggacg
     1561 acatcctctg ctccgaaact gcaggccagc acgatctcag gcgcatatcg ttcgattgcc
     1621 gccgccaaca cctcctgcgg ctgcttcgct gcgaacgact ctccccaggc cttcacttct
     1681 tctggcgaca acttcgtcat ctgatccctt cttcgatcta ccacaacgcg cggcataggg
     1741 ataatgcccg agcgcgcgcg tcattctcac ccgcccccca gacgcgaaat gcgccttgac
     1801 ccgagcgagg ccttccaagg ccacgccttg ctcgagcatg ggcattatcc ctatgccgca
     1861 tcactctact ttttcgacca acacccgata atgtgccgct tccggttcga gggcttcttg
     1921 caggaggacc ttgtgcccgt cattttttaa gctcatcggg acattcttaa tcggctcccc
     1981 tgcgtcgagc cagacttcta actgctcacc ggcgtccatc atctcgagtt tcaacttcgt
     2041 cttcacatag ttcagcgggc aggctacgcc gcgaagatcg taaaccggcg caccctttga
     2101 tgtaggggca gacaccaccg ctgcaatgtc cttcactggc agcacaaccg gcttggggac
     2161 aggggacgct tcgccctctc ctgcaag
//
LOCUS       DATDNW010000132         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_629491, whole genome shotgun sequence.
ACCESSION   DATDNW010000132 DATDNW010000000
VERSION     DATDNW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629491"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VI359_03315"
     CDS             complement(<1..411)
                     /locus_tag="VI359_03315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973315.1"
                     /translation="MALWHSIAQQFRQPAGWVGRLAGFFFRINREGIDWTINLLEIQP
                     TDHVLEIGFGPGHGIQQAASLATQGRVAGVDFSHTMLEQASQHNAMAIDSGLVELCLG
                     DATSLLYPENTFHKVFATNVIYFWSDPLVNLQELR"
     gene            892..1215
                     /locus_tag="VI359_03320"
     CDS             892..1215
                     /locus_tag="VI359_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973316.1"
                     /translation="MTEHRSQFRFPVERRALLKHNGNTILCDVLDLTEQGLQLRTEFP
                     IAVGDTIQLECQLDTHTIIHCALLVTHAAPPHVGGRITDISTEHHQQLMRYIQQLSAQ
                     NLGGM"
     gene            complement(1236..2255)
                     /locus_tag="VI359_03325"
     CDS             complement(1236..2255)
                     /locus_tag="VI359_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY6973317.1"
                     /translation="MLRLILLFIGLLTLGFLVWHIGPGNIYEAAARLGPVALLAILVP
                     SLIMYVIEAYGWKVTLGPTAKHVPFWRLLAIRTAGEVVNMTTPTAYVGGEPLKAYLLR
                     KHHVPMVEGLASVVIAKTTMTIAEVLFILLGIALAFWALGANGSSGHTIAAALLSVGL
                     LLFGTAAFVFVQRKGLFTWILEFLRKIGLRIGFLEARADQLRSLDRTILDFYTHNRPA
                     FYASTGLFFLGWLAEALEVFVMIYYLGGPSVMLSAISIGALSVFIKGGTFFIPGSLGA
                     QDGGNLLLLKAFGYSDVTGITFALLRRFRELVWIGLGLLCLALVGGRAAATQEQRAHD
                     SGTSS"
     gene            complement(2458..>2887)
                     /locus_tag="VI359_03330"
     CDS             complement(2458..>2887)
                     /locus_tag="VI359_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973318.1"
                     /translation="VARLTFTESPFGAWLDIALDNVVHMAIFVGIAAGSYLRVMGHEG
                     AWVPLVLGAAAVLGNAVSFTLVSKAQKIKTASEWKTPVHAAWSDFMLKNVASRDFSVI
                     VLLFACLDRLEWFLWMAAIGSMSFVAVMLWVLRPSAVTRA"
BASE COUNT          657 a          875 c          802 g          553 t
ORIGIN      
        1 ccggagttcc tgtaggttca ccagcggatc gctccagaaa tagatgacgt tcgtcgcgaa
       61 cactttgtgg aaggtgtttt ctggatagag cagtgaggtg gcgtcgccga ggcacagttc
      121 gaccagtccc gaatcgatgg ccatggcatt gtgttgagag gcttgctcca gcatcgtgtg
      181 agaaaaatcc acaccggcga ctctgccctg ggtggccagg cttgccgcct gctgaatacc
      241 atgacccggg ccaaagccga tttccagcac gtgatcggtc ggttgaatct ctagcagatt
      301 gatggtccag tcgatcccct cgcgattgat gcggaagaag aaacctgcga gccgcccaac
      361 ccatccagcc ggttgtcgaa attgttgtgc aatgctgtgc cacaatgcca tatactcccc
      421 cagttatcta cgtaggccag cactatatcg tgacgaaatc agccgattaa cttttcgtct
      481 tcgtgcccct taaggggcag gaaattcttg ctgaatgctg ccttttgctg tcgtcttttt
      541 gctcaggatt tccctcgtcg atgcccgctc tcgttggcca ccgcctcatc catcgaatgg
      601 cctctatgaa tcaactggtt ctgaggagcc catctttctg gttatacggc tggtccccgc
      661 attgcacttt tcctggtcaa aactacggac gaaaggagac ccgccatgtc gtcgctcatg
      721 cttatcatac tggtcagtgc cttcccgttc ccagtgttgg gactcatgct tgcggtcgat
      781 cgggtgaagc atgaaaggcc acaccacagg ccttgacaca accttcgctt tctggctggt
      841 cttccctggc catggcactc agtcgccacg tgactggggg agacccactt catgactgaa
      901 caccgcagcc agttccgctt ccctgtcgaa cgtcgcgccc tcctgaagca caacgggaat
      961 accatcctct gcgatgtttt ggacctgacg gaacaagggc tgcagctccg gacggaattc
     1021 ccgatcgccg ttggggacac aatccagctc gaatgccagc tggacactca caccatcatc
     1081 cattgcgcgc tgctcgttac ccacgccgcg ccccctcacg tgggcggccg gattaccgac
     1141 atctccaccg agcaccacca acagctcatg cgctatattc aacagctaag cgctcagaat
     1201 ctggggggca tgtagcggga gatgcgaagg agggctcagg aactcgttcc tgagtcgtgc
     1261 gcacgctgtt cttgagtcgc cgcagcccgc ccgccaacga gagcaagaca cagcaatcct
     1321 aatccgatcc agacgagttc gcggaatcgc cgcagcaggg caaaggtgat gccggtgacg
     1381 tccgagtagc cgaaggcttt gaggagcagc agattgccgc cgtcctgtgc gccgaggctg
     1441 ccggggatga agaacgtgcc gcctttgatg aaaactgaga gcgccccgat ggatatggcc
     1501 gagagcatca ccgacggccc gccaagataa tagatcatga cgaacacttc cagggcctcc
     1561 gccagccagc cgagaaagaa cagccctgtc gacgcataaa acgctggacg attgtgggta
     1621 tagaaatcca gaatcgtccg atcaagcgac cgcagttggt ccgcccgcgc ttctaaaaac
     1681 ccgatcctga gtccgatctt cctcaagaac tccagtatcc aggtaaaaag gcccttccgc
     1741 tgcacgaaga caaacgcggc cgtgccgaac aacaagaggc cgacactgag caaagcggcc
     1801 gcgatcgtgt gccccgatga cccgttggcg cccagtgccc agaacgcgag cgcgatgccg
     1861 agcaaaatga ataggacttc tgcgatcgtc atcgtcgtct tggcaatgac gacagaggcc
     1921 aatccttcca ccatgggcac gtgatgcttt ctgagcagat aggccttgag cggttcgccg
     1981 ccgacgtacg cggtcggcgt cgtcatgttt acgacttcac cagccgttcg gatcgccagc
     2041 aatcgccaga agggcacatg cttcgcggtg ggaccgagcg tgaccttcca gccataggct
     2101 tcgatcacat acataatgag ggacggaacg agaatggcga gaagcgcgac tgggccaagc
     2161 cgggccgccg cctcatagat gttgccgggg ccgatatgcc aaacgaggaa cccgagtgtg
     2221 aggaggccga tgaaaaggag aataagtctg agcacgtggg gttagaatcc gtcattcgat
     2281 acgggatgct caaaacgacc tattatcatc acccgcccgc ccctaggcgc gccaagacgc
     2341 gccgattgac ccagcgaggt cgcaaagagc gaaggccccg agccgtacgg ttgtcggtac
     2401 gtcgagggga ataagcgact gagaacaaag catggtgtcg gttttagcat cccgctacta
     2461 agcacgggtc acagctgacg gacggaggac ccacagcatc acagcaacaa acgacatcga
     2521 tccgatggcg gccatccaga gaaaccactc cagcctgtcc aagcacgcga agagcagcac
     2581 gatcaccgag aagtcccggc tggcgacgtt cttgagcatg aagtccgacc aggcagcatg
     2641 caccggcgtt ttccattcac tcgcggtttt gatcttttgc gctttgctca ccagtgtgaa
     2701 cgatacggcg tttccgagca ccgccgccgc acccaagacc agcggcaccc atgcgccctc
     2761 atggcccatt acccgcaagt acgagcctgc ggcgatcccg acgaagatgg ccatatgcac
     2821 gacgttgtcc agcgcgatgt ccagccaggc accgaaggga gattccgtaa acgtgaggcg
     2881 cgcgact
//
LOCUS       DATDNW010000133         3734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1311074, whole genome shotgun sequence.
ACCESSION   DATDNW010000133 DATDNW010000000
VERSION     DATDNW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311074"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..874)
                     /locus_tag="VI359_03335"
     CDS             complement(245..874)
                     /locus_tag="VI359_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY6973319.1"
                     /translation="MMPKNLPGILTGRVNFFSRWFRKRSYFQIELSSRYRWYLAPLRL
                     VLMATCGVLGATMAWDIAQTSITYQEASAIQARIKSVQEQDQQLLVEAKDHDIDLSES
                     SLQRLPAEVALANQLLAKRHFSWTQFLSGLEEVIPPRVAIESVRLDPGSAVIHLTGSA
                     VSLEDITALTVKLQDHQIFRDPVLGQHRAGSAGLVEFDLMLKYRPPQGI"
     gene            complement(878..1876)
                     /locus_tag="VI359_03340"
     CDS             complement(878..1876)
                     /locus_tag="VI359_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973320.1"
                     /translation="MWEFAASRPRLCVKIGAQALAWVEASRNWRGRRRYRCLLSPTPT
                     GAVKLTPVELNLTDVAVVENRLRSFAGPNRDLRVGGQVLLADIPRPITLLLPDLSVRA
                     TVVHLEQFPDRPEEQEALVRWRLGQEQLFSLAGVKVFWQVFPPERSTHQRTYAVLVVA
                     IQESVLNQYEAVCESAGLVTQQIGVCSLRLFNLWLKASKGRRLDRDLMWVSVLDGGLT
                     CLVLHEGRVVFVRTKRLEGGPVQLEEEAGGEFADRIVKECAASLYMCHEHHPKLAVKY
                     VVYASDHPISGLDATFSRELYVSTEQCDWEQVEELGWRYDGGSMSLAAFPVLAGVV"
     gene            complement(1880..3571)
                     /locus_tag="VI359_03345"
     CDS             complement(1880..3571)
                     /locus_tag="VI359_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY6973321.1"
                     /translation="MLVKLGMVPKQTVEQTLGRLGGAVAALGQTLLSEGALSEEQLAR
                     ALASQFDLPFDPLTDFRVDRRFYESMSVKLMQRHPFVPMTEQQGRLTIAIPDPQNLLA
                     LDQLELLLARPLDLVVSPRSAILVALEHSEGSSQALRELEAEYRSVLVKEDERGEEVL
                     TIDEAGEDQSPAVKLLDSILLSAMQRRASDIHIEAADRATKVKFRVDGILVPAMEPLD
                     IRLHAPLVSRLKVMSDLDIAERRVPQDGSFRMRLERKTVDFRVSILPSVFGESVVIRI
                     LDREAITAGVSTLRLDRLGFNEEDLKRFRRAITRPYGMVLVTGPTGSGKTTTLYAAIT
                     EMNIKEDKLITIEDPVEYQLPGVVQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRD
                     AETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDAYNFLAALNCVLAQRLVRI
                     SCPACRVPIKVEKSLAEESGLDYEEYKDLTLYQGKGCQDCHGTGYRGRKCITEFLDLT
                     DEIKEMILAERPLSEIRYRAVTAGMITLRQSALKKVLAGDTTLHEINRVTFSEES"
BASE COUNT          858 a         1105 c          966 g          805 t
ORIGIN      
        1 ggcaagacag cccacacttc gacgagatcc tgacgcgctt tcttggcttc tttgtggtgt
       61 gtcaatcgct ggcgggcctg cgcccactcc gattccaatt gagccctatg tcgttccgcc
      121 ccttcgagtc cgaccgcatg ggccaacaat agtccagcga agagtacgag catgacgagg
      181 caccagggga ccagtggtgc gtagggactt cgcagctgtt gggtaattat attcatcgct
      241 cgccctagat accctgtggc ggccggtact tgagcatcaa atcaaattcc accaaccccg
      301 cggatcccgc acggtgctgc cctagaactg ggtcacggaa gatctggtgg tcttgcaact
      361 tcaccgtgag tgccgtgatg tcttcaaggc tgacggccga gccggtgaga tgaatgaccg
      421 cactccccgg atcgagacgc acactctcaa tggccacgcg tggtggaatt acttcctcaa
      481 gaccggagag aaattgtgtc caagagaaat gtcgttttgc gagaagttgg ttggccaacg
      541 ccacttcagc cggcaatcgt tggagggacg attccgagag atcaatgtcg tggtcttttg
      601 cctccaccaa cagctgttga tcctgctcct gtacactttt gatgcgcgct tgaatagcag
      661 atgcttcttg ataggtgatg ctcgtctgag cgatatccca tgccatcgtg gcacccaaca
      721 ccccacaggt cgccatgagg acgagtcgga gaggggccag ataccagcga tatcggctgc
      781 tgagttcgat ctggaaatag gatcgcttcc ggaaccatct cgagaagaaa ttcacgcgcc
      841 ccgtgagaat gccgggcaag ttctttggca tcatgattta taccactcct gccagaacgg
      901 gaaatgcggc aagggacatg ctccctccgt catacctcca tcccaattct tcgacctgct
      961 cccaatcgca ttgctctgtc gaaacataaa gctcccgact gaatgttgcg tctaagccag
     1021 agataggatg gtcactggca tagaccacgt atttcacagc aagcttcgga tgatgctcat
     1081 ggcacatata gagcgaggcc gcacattcct tcacaattct gtccgcgaac tcgcccccag
     1141 cctcttcctc caattggaca ggccctcctt ccagtcgttt cgttcgcacg aacacgaccc
     1201 gcccctcgtg cagaacgaga catgtgagcc cgccgtccaa cacgctcacc cacatgagat
     1261 cacgatctaa tcgccggcct tttgaagctt tcaaccagag attgaagagc cgtaaactgc
     1321 agacgccaat ctgttgcgtg accaatccgg ctgattcaca caccgcctca tactggttta
     1381 ataccgattc ttgaatcgca acaaccagca ccgcgtaagt tctctggtgc gtcgaccgtt
     1441 caggaggaaa gacctgccag aacaccttga ctccagcgag agagaaaagt tgttcttgac
     1501 caagacgcca ccggaccaac gcttcttgct cttcaggtcg atcaggaaat tgctcaagat
     1561 gcacgacagt ggcgcggacg gagaggtctg gcaagagcag tgtgattggg cgtggtatgt
     1621 cggccagcag cacctgtccg ccgacgcgca ggtcccgatt cggtccagca aatgatcgga
     1681 gacgattctc gaccaccgcc acgtcggtaa gattcaactc aaccggcgtg agcttcaccg
     1741 ctccggtcgg cgttggcgac agcaggcatc ggtagcgacg tctaccccgc cagtttctgg
     1801 acgcttcaac ccatgccagc gcctgagcgc cgatcttgac acagagacga ggtcgactgg
     1861 cggcaaattc ccacatgcgc tagctctcct cactgaatgt gacacgattg atttcatgca
     1921 gcgtggtgtc gccagccagc actttcttca gcgcagactg ccgcaacgta atcatgccgg
     1981 cggttaccgc gcgataccga atttccgaaa gcgggcgctc cgcaagaatc atttccttga
     2041 tctcatccgt cagatcaaga aactcggtga tacattttcg tccccgatag cccgtaccat
     2101 ggcaatcctg acagcctttc ccttgataga gcgtgaggtc cttgtattcc tcataatcga
     2161 gacctgattc ttccgcgaga gatttctcaa ctttgatcgg cacacgacac gcaggacaag
     2221 aaatccgaac aagccgctgt gccaacacgc agttcagcgc ggcgaggaag ttgtaggcat
     2281 caatccccat cgaggcgaat cgaccaatca cgtcgaagac attgttggcg tggactgtgg
     2341 tcagcaccaa atggcccgta agggcggatt gaatggcaat ctgtgccgtt tcagcatcac
     2401 gaatctcacc gaccatgatt ttatccggat cgtgacgcag gatagatcgc agccctcttg
     2461 caaaactcag cccttttttc tcgttgactg ggatctgcac gacaccaggc aactgatact
     2521 ccaccggatc ttcaatggtt atcagcttgt cctctttgat gttcatctca gtgatcgcag
     2581 catagagggt cgtcgtcttt ccgcttccgg tcggaccggt gaccaacacc atgccgtacg
     2641 gtctggtaat cgctcggcgg aatcgtttga ggtcttcttc attgaacccc agtcggtcta
     2701 accgcaacgt ggataccccc gctgtaatcg cttcgcgatc caagatacga atgacgactg
     2761 actcgccaaa tacgctgggg agaatcgaca cacggaaatc gacggttttc cgttcaagcc
     2821 gcattcgaaa actgccgtcc tgaggtacac gccgttcggc aatgtcgaga tcagacataa
     2881 ctttgagccg agacaccaaa ggggcatgga gccgaatgtc caatggttcc atcgctggaa
     2941 ccagtatgcc atcgaccctg aacttcacct tggtggcgcg gtcggcagct tcaatatgga
     3001 tgtcgctggc tcgtcgctgc attgcgctca acaagattga atcgaggagc ttgacggcag
     3061 gactctggtc ttctcccgcc tcgtcaatgg tcaagacctc ttctccccgt tcgtcttcct
     3121 taaccagtac agaccggtat tctgcctcta gttcccgcaa cgcttggctg gatccctcgc
     3181 tatgctccaa agccaccaaa atggcgctgc gagggctcac gacaagatcg agcggccgcg
     3241 ccaggagaag ttccaactga tcgagcgcca ggagattctg aggatcggga atcgcgatgg
     3301 tcagacgtcc ttgctgctcg gtcatcggca caaagggatg ccgctgcatc aatttcacag
     3361 acatggactc atagaaacgc cggtcgacgc gaaagtccgt gaggggatcg aacggaaggt
     3421 caaattgtga ggccaatgcg cgcgccagtt gttcttcgga aagagcgcct tcgctgagca
     3481 acgtttgacc gagagccgcc accgcgccgc cgagccgacc cagcgtctgc tcaactgtct
     3541 gtttcggaac cattccgagc ttgacaagca cgtccgccag cgatggccgg gcaagagtcg
     3601 gaacagcgac ggctcgtgca ctacgactgc cttcttgagt cgaccgagca ggtcgtcgat
     3661 tcggaacaag cctcatacac tccctgccat ctgaaagacg ggaagataca tcacaatgac
     3721 gattaccccg acca
//
LOCUS       DATDNW010000134         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1106263, whole genome shotgun sequence.
ACCESSION   DATDNW010000134 DATDNW010000000
VERSION     DATDNW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106263"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1762)
                     /gene="zwf"
                     /locus_tag="VI359_03350"
     CDS             complement(221..1762)
                     /gene="zwf"
                     /locus_tag="VI359_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY6973322.1"
                     /translation="MPTTPRIDIPPARETLPPVDPCTLVIFGGSGDLARRRLIPALYN
                     LLLDGLLPSNFAVLGLGRKPMSDEEFRGIAREGVMAHSRQALVETTWNEFAAHLSYIA
                     GDNNDPNTYATLKSKAESVEQQFQIPGNRIFYLSIPPSSFTSVCAGLDRAGLVTRDGN
                     RSPYTRIIVEKPVGRDLVSARQINTVTGQVFDESQIFRIDHYLGKETVQNLMVVRFAN
                     SIFEPIWNHKYIDHVQITVSEAEGVGSRATYYEEAGALRDMVQNHLLQLLCLVAMEPP
                     YSLDPDVVRNAKMEVLRCLRPILGKDVEQCTVRAQYTEGSAHGKPIPGYRREPGVNPN
                     STTETYVAVKCFVENWRWSGVPFYLRTGKALPCRASEVAVQFKDIPQILFNANARQPQ
                     PPNVLALRIQPEEGLSLRIVSRVPGTREQTHPVEMDFQYSDVFGRPSPEAYERLLLDV
                     MAGDASRFMRRDAVEASWAWITQILDGWQQQGLRWLPAYQAGTWGPVEADRLIQQDGR
                     TWRTL"
     gene            complement(1852..2637)
                     /locus_tag="VI359_03355"
     CDS             complement(1852..2637)
                     /locus_tag="VI359_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973323.1"
                     /translation="MDTNKSGTLPTREELNAELLEVPEPPEQPEAVNEPAYEDIVAVA
                     LRHVRGRRGGDLVSVILVGSGARRAVTAHSDVDLIALVKGQAEGHEIIRIADRLADIR
                     YRGHQEVEEELPYSPRLPPLLRKGRILYDHDGIGANLIERANQRFRQGPPPTSMNEQI
                     RMKAGCLHWLGKAQDIADKPATANYLLMLFFDDFVNAFFRLRGLWLTAPVDVPRFITS
                     RDAVLGDLAGRFLIATTLAERLNCARDLVPLLFKDVPNPARID"
     gene            complement(2638..2877)
                     /locus_tag="VI359_03360"
     CDS             complement(2638..2877)
                     /locus_tag="VI359_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973324.1"
                     /translation="MEYLMPLQVVSFAQISRILEITDSLGLNREWVEIPLSPESPGAV
                     RRLTNGKLEIIVDADQPFDEWIGSLPKHIQLAQKI"
     gene            complement(2928..3959)
                     /gene="ltaE"
                     /locus_tag="VI359_03365"
     CDS             complement(2928..3959)
                     /gene="ltaE"
                     /locus_tag="VI359_03365"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY6973325.1"
                     /translation="MIDLRSDTVTKPSDAMRKAMARAEVGDDVYGEDPTVNRLQEMVA
                     AMLGKKGALFVPSGTMANQLSIRIQTQPGQEVIVESKSHIVRYEQGAAGALSGVQLHW
                     VTGERGLMTAEQIEAAIRPKDPYSIRTALICLENTHNAGGGTIYPLSTIERIREVALR
                     HNIPMHLDGARLFNAVAATTLPPASYAQNFETVSLCLSKGLGAPVGSLIVTNDSALID
                     KARRFRRMYGGGMRQAGILAAAGIYALEHNIGRLKEDHDNAKRLARKLQQIPSVSINL
                     QHVDTNIVIFDVVNHRLSPTALVTALKQEGVLINAIGGSSFRAVTHLDVSAKQIDEAA
                     EIFTRILGN"
     gene            complement(3956..>4679)
                     /locus_tag="VI359_03370"
     CDS             complement(3956..>4679)
                     /locus_tag="VI359_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973326.1"
                     /translation="KKFEPDNPTIQKEFPGACAPFMAARTNAFHLLLPFDLKISRTPE
                     DPLEAGVRIFYAKPGYSYPLRYEMGKLCSYHDGQMLDIALDDPNLFFVSVSEIKEPAF
                     RYIPPDRAPDVPDELAYPLSVLNMVGSLGPFIQISCKFKVWFDASVVSLLIQGAPDLA
                     EYGLQGASGLMTRTYASDRVEAYAESFRQPWQEGLSYNFVNLHLGLLPDTQTALIPFN
                     TPIFTIYPVLSRHAYNFEEKPA"
BASE COUNT          959 a         1297 c         1416 g         1007 t
ORIGIN      
        1 gctgaaaatg aacgtcgaag agaactatca gattcctgcc gaattggagg tgtcctcgtt
       61 gccggcgctg gcgctctatc gcaacgggtt gttcgagcga ttcatcggtg gactgggcac
      121 ccgaacggag atcctgaaac aactgcggct ggctacgagc agttagaaat ggcagacagc
      181 gcgagcatcg tctgctttct gtttccggct tatgccgatg tcagagcgtg cgccacgtgc
      241 gtccgtcttg ctggatgagg cggtccgctt cgactggccc ccatgtcccg gcttgatagg
      301 ccggcagcca acgcaggccc tgttgttgcc agccgtcgag gatctgggtg atccaggccc
      361 atgaggcctc cacggcatca cgccgcatga accgtgatgc atcacctgcc ataacgtcca
      421 ataacaaccg ctcataggct tcaggtgatg gtcggccgaa gacatcgctg tactgaaagt
      481 ccatctccac cggatgggtc tgctcgcgcg tgccggggac ccgcgacacg atccgcaacg
      541 aaagtccctc ctccggttgg atgcgaagcg cgagcacatt gggaggctga ggctggcgcg
      601 cattggcatt gaacaggatt tgcgggatgt ccttaaactg gacggcgact tcgcttgcac
      661 gacagggcaa cgccttcccg gttctgaggt agaacggcac gccggaccag cgccaatttt
      721 caacgaagca cttcacggcc acataggtct ctgtcgtgga attcgggttg acgcccggtt
      781 ctcgtcgata accgggaatc ggtttgccgt gtgctgatcc ttcggtgtat tgggcgcgaa
      841 ccgtacactg ttcgacgtct ttcccgagga tgggacggag acagcgcagg acttccatct
      901 tggcgttgcg gacgacgtcg gggtcgagag aatagggcgg ttccatcgca acgagacaga
      961 ggagttgaag caggtgattt tgcaccatgt cgcgcagcgc accggcttcc tcataatagg
     1021 tggcccgtga tccgacgcct tcggcttcgc tcacggtaat ctggacatgg tcgatgtact
     1081 tatgattcca gatcggctcg aaaatgctat tggcaaaccg gacgaccatc aggttctgga
     1141 ccgtctcctt accgagataa tggtcgattc ggaaaatctg cgattcgtcg aagacttggc
     1201 cggtgacggt gttaatctgc cgtgcggaga cgaggtcgcg gccgaccggt ttttccacga
     1261 tgatccgggt ataggggcta cggttgccat cgcgtgtcac aagcccggcc cggtcgagtc
     1321 ccgcgcagac cgacgtgaac gagctcggtg gaatgctgag ataaaaaatc cgattcccgg
     1381 gtatctgaaa ttgctgttcg acagattccg ccttcgattt cagagtggcg tacgtgttcg
     1441 ggtcgttgtt gtcgcctgcg atgtaagaca gatgcgccgc aaactcattc caagtcgtct
     1501 ccaccagcgc ttgccgtgag tgggccatga cgccttcgcg agcgattcca cgaaactctt
     1561 catcggacat cggtttgcgg cccaagccca ggaccgcaaa attcgacggc agcaatccgt
     1621 ccagcaacaa gttatagagc gcgggaatta gccggcggcg agccagatcg ccggatccgc
     1681 cgaaaatgac cagcgtgcag gggtctaccg gtggcagcgt ctctcgcgcc ggggggatgt
     1741 cgatacgcgg tgtcgttggc atcacaacct cattctaacg gcggaccgga ttactgccaa
     1801 gggcatgtcg agcggcagcc gatcatccgg cttcatcacg tccgagtcag ttcaatcgat
     1861 ccgagctgga ttgggaacat ccttgaataa gaggggcacg agatcgcgag cacagttcag
     1921 ccgttctgcc aacgtcgtgg cgattaaaaa tcgtccagct aagtccccga gcacggcatc
     1981 acgggaagtg atgaatcggg gaacgtcgac gggcgccgtc aaccacaagc cgcgcagtcg
     2041 gaaaaacgca ttcacgaaat cgtcgaagaa caacatcaac agatagtttg cagtcgccgg
     2101 cttgtcggct atgtcttggg ccttgccaag ccaatggaga caacccgcct tcatccgaat
     2161 ttgctcgttc atgctggtgg ggggaggccc ctgacggaat cgctggtttg cccgctcgat
     2221 caaattggcg ccgattccgt cgtgatcgta aaggatgcgc ccttttctca ggagcggcgg
     2281 aagacgaggg gaatagggaa gttcttcttc aacctcttga tgccctcgat atctgatgtc
     2341 ggcaagacga tccgcgatgc ggatgatctc atgtccttcg gcctggcctt tgacgagcgc
     2401 aatcagatcg acgtcgctgt gggctgtcac ggcgcgtctc gctcccgaac cgacgagaat
     2461 cacgctgacg aggtcgccgc ctcgccggcc gcggacatgt cgaagggcaa cggcgacgat
     2521 gtcttcatag gccggttcgt taaccgcttc aggttgttcg ggcggttccg ggacttcgag
     2581 gagttccgcg ttgagttctt cccgtgtcgg gagggtaccg gacttgttcg tgtccatcta
     2641 gatcttctgt gccaattgaa tatgtttcgg aagtgagccg atccattcgt cgaatggctg
     2701 atcggcatcg acaataattt ccagcttacc attggtcaga cgccgtaccg ctccaggact
     2761 ttccggggac aatggaattt ccacccattc ccggttcagc ccgagcgagt ccgtgatctc
     2821 gaggattcgg ctgatctgtg cgaacgacac tacctgtaac ggcatcaggt attccacaca
     2881 cgtgcattct agatttcagg ggaagaacgt gtcaatgcgc gcgccgctca gttccccaag
     2941 atccgtgtga agatttcggc cgcctcatcg atctgtttgg cggacacatc gagatgggtg
     3001 acggcgcgaa agctgctgcc gccgatcgca ttgatcaaga caccttcttg tttgagggcc
     3061 gtgactaggg cggtaggaga gaggcggtga ttgacgacgt cgaagatcac gatgttggtg
     3121 tctacgtgct gcagattgat agagaccgac gggatctgtt gaagtttgcg ggccagccgt
     3181 ttcgcattgt cgtgatcctc tttcaggcgg ccgatattgt gttcaagagc atagataccc
     3241 gctgcggcca gaataccggc ctgcctcatg cctccaccgt acatgcggcg aaaccgtctg
     3301 gctttatcga tcaacgcgga atcgttagtg acgatcagcg agccgaccgg cgcgccgagc
     3361 cctttcgaga gacagagcga caccgtttca aagttttggg cgtaagacgc cggtggcaac
     3421 gtcgtagcgg cgacggcgtt aaataagcgc gcaccatcca agtgcatcgg gatgttgtgg
     3481 cggagggcga cctcgcgaat ccgttcaatc gtcgagaggg gatagatcgt gcctccgccg
     3541 gcattgtgcg tgttctccaa acaaatcagc gcggtcctga tggaatacgg gtccttgggg
     3601 cggatcgctg cttcgatctg ctccgccgtc atgaggccac gctcgccggt cacccagtgg
     3661 agctgcacgc cggaaagggc tccggccgct ccttgctcgt agcggacgat gtggctttta
     3721 ctctccacga tcacttcctg gcccggttgc gtttgaatac ggatcgacag ttgattcgcc
     3781 atcgtgcccg aggggacgaa gagcgcccct tttttcccca acatggctgc gaccatctct
     3841 tggaggcgat tcacggtggg atcttcgcca tagacatcgt cgccgacctc ggcacgggcc
     3901 atggccttcc gcatcgcatc ggacggtttg gtgaccgtgt cgctgcgcag atcgatcacg
     3961 ccggcttctc ctcgaagttg taggcatgcc gactcaacac cgggtagatc gtaaagatcg
     4021 gtgtgttgaa cgggatcagc gcggtctgcg tatcgggcag cagtccaaga tggaggttca
     4081 caaagttgta gctcaaacct tcttgccaag gctgccggaa tgattccgca taagcttcaa
     4141 cgcggtcgga ggcgtaggtg cgtgtcatca gccccgaagc tccttgcaga ccgtattccg
     4201 ctaagtccgg tgccccttga atcagcagcg agacgactga tgcgtcgaac cagactttga
     4261 atttgcagct gatctgaata aagggaccca gtgagccgac catgttcaac acggagagcg
     4321 ggtaggccaa ttcgtctggc acgtccggtg cgcgatccgg cgggatatag cggaaggcgg
     4381 gctccttgat ttcagacacg gaaacaaaga agaggttggg atcatccagc gcaatgtcga
     4441 gcatctggcc atcgtggtag ctgcagagtt tgcccatttc ataccgcagc ggatacgagt
     4501 accccggctt ggcgtagaag atacgcacgc cggcctcgag gggatcttct ggcgtgcggc
     4561 tgatcttcag gtcgaacggc agcaggagat gaaaggcatt ggtccgcgca gccatgaaag
     4621 gggcgcaggc gccgggaaat tccttttgga tcgtcgggtt gtccggttca aactttttg
//
LOCUS       DATDNW010000135         5723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_698495, whole genome shotgun sequence.
ACCESSION   DATDNW010000135 DATDNW010000000
VERSION     DATDNW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698495"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VI359_03375"
     CDS             <1..459
                     /locus_tag="VI359_03375"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY6973327.1"
                     /translation="DGGVKYSGDITKALAAGASSVMLGGLLAGTEESPGETVLYQART
                     YKVYRGMGSIGAMERGGGDRYGQAGRQAQKLVPEGIEGRVPYKGPVSAVIYQLVGGVK
                     SGMGYCGCKTIPELQQKATFIRQTVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            607..2163
                     /gene="guaA"
                     /locus_tag="VI359_03380"
     CDS             607..2163
                     /gene="guaA"
                     /locus_tag="VI359_03380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY6973328.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAHVYSQIMPCTVPLATV
                     LAYRPKGIVLSGGPSSVYDRKAPSVAKELFAQGIPILGICYGMQLVTHLSGGEVAKAS
                     HREYGRAELTIDDRSDLFKGVGEGGSTIVWMSHGDRIERMPPGFRSIAHTGNSPIAAM
                     RRHDGKGRIYCLQFHPEVAHTPEGATVLRNFVYDICGCQPSWTMRSYVDTAVEQIRKQ
                     VGKDQVICALSGGVDSSVAAVLTHRAIGDQLTCVFVDNGVLRAGERDQVQKTFAANFK
                     LNLRILDRTNEFLAGLKTVIDPERKRKIIGRLFIKNFEAESKKLKGVKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPARMKLKLIEPLRELFKDEVRVLGKELGIPDDVIW
                     RQPFPGPGLAIRILGSVTKERLTILREAETIVEQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYENVVAIRAVTSLDGMTADWAKIPNEALGKMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            2175..2993
                     /locus_tag="VI359_03385"
     CDS             2175..2993
                     /locus_tag="VI359_03385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY6973329.1"
                     /translation="MEIRLQKLIAGTGLASRRKAETLISAGRVTVNGRVVTELGTKVD
                     PIRDHVKVDGKHLSSAQPYVYLMLNKPKHVMSTLDDPGGRPTVKDYLRGVSVRVFPVG
                     RLDFDSEGLMLLTNNGDLAQALLHPRYHVPKTYLIKVKRVLTDEEIRTLERGVKLDDG
                     MTSPAHVKKVKKVEQNSWLELTIREGRKHQVKRMLDAVGHPVLRLIRVKMGPLVLGDL
                     EPGEFRFLEDREANALRELVEQRQQSVQEGRFPAETKKPARRAGWARPKKVNVA"
     gene            2990..4753
                     /gene="aspS"
                     /locus_tag="VI359_03390"
     CDS             2990..4753
                     /gene="aspS"
                     /locus_tag="VI359_03390"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY6973330.1"
                     /translation="MKTRTHRCGELNKAHVGQTVVLNGWVQRHRDHGMVMFIDLRDRT
                     GITQVVFNAERNAAVHQAAHSLRSEYVVSITGQVMARPAESRNPNLPTGDIEVFVDAV
                     EVLNEAKTPPFVIEDDADVTEAVRLKYRYLDLRRPKMQRLLAMRHNVTQAVRSFLNRE
                     GFLEIETPILTKSTPEGARDYLVPSRVNPGMFFALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDRDQVMSVMEQMIVTVFREAGGVQLPKPFPRM
                     TYAEAMGRYGSDKPDLRFDMPLHDVTAFGAASEFKVFREAATKGKIVKAMIVKGGGTI
                     ARSRIDALGETAKSFGAKGLAWLKITTDGQLDSVIAKFLDAKAFFAALPEAKPGDLVL
                     FGADKSAIVHDVLGRIRLLLGEELNLIDKTAWKPVWVTEFPLLDYSEEEKRYVFMHNP
                     FAAPMDEDLALLDAEPLKARAKAYDMVLNGNEVGGGSIRNHRSEIQLKILDLLGISKD
                     QAQAKFGFLLEALDYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQKAQCPLTD
                     APSSVGPDQLKELRIKLDLVE"
     gene            4750..>5723
                     /gene="aroC"
                     /locus_tag="VI359_03395"
     CDS             4750..>5723
                     /gene="aroC"
                     /locus_tag="VI359_03395"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY6973331.1"
                     /translation="MNGPHVHIMAGNTFGRLFTVTSFGESHGPAIGCVVDGCPPGMML
                     SADDVQKDLDRRKPGTSRHVTQRQESDTVEILSGVFEGKTTGTPIALFIRNEDARGRD
                     YGNLVDTFRPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLSEKHGVV
                     IRGYLSQLGPIPIPFKTWSAVSTNPFFAADPDIIPKLEAFMDDLRKSGDSVGAAITTV
                     AEHVPVGLGSPVYAKLDADLAAAMMSINAVKGVEIGSGFASVTQCGSEHCDELTPEGF
                     LTNHAGGILGGISTGQNVVVTIGIKPTSSVRLPRRSIDKQGNPVTVET"
BASE COUNT         1260 a         1544 c         1774 g         1145 t
ORIGIN      
        1 gacggcggcg tcaaatattc cggggacatc accaaggccc tcgcggcagg cgcgtcctcg
       61 gtgatgcttg gcggcttgct ggccgggacg gaagagtcac ccggcgagac tgtgctatat
      121 caagcccgaa cgtataaggt ctatcgcggc atggggtcga tcggtgcgat ggagcgcggc
      181 ggcggagatc gttacggaca ggccgggcgt caagcgcaga agctggtgcc ggaagggatc
      241 gagggacgcg tgccctacaa agggccggtg tcggccgtga tttaccaact tgtcggcggt
      301 gtgaaatccg gtatgggcta ctgcgggtgc aaaacaattc cagaactgca gcaaaaggcc
      361 acgttcatcc gccagacggt cgccggcctc cgcgaaagtc atgtccatga cgtgatcatt
      421 acgaaagagg ccccgaacta tcggatggat tgggagtgat cgaaagctat cagctctcag
      481 ccgtcagctt tcaaccagac acacatcatt cccgcctcgg ttcagatctt gtcaccagca
      541 cgtgtgcagt tgccctatct aaattgttcg ccggtagctg aatgttgacc gccgaaagct
      601 aatacgatgg aactctggca cgatcgtatc ctggtcctgg atttcggctc gcaatacacc
      661 cagctcatcg cgcgtcggat ccgcgaggcg cacgtctatt cccagatcat gccctgcacc
      721 gttccgctcg cgacggtcct ggcctatcgt ccgaagggca ttgtgctgtc cggcgggcca
      781 tcgagtgtct atgacaggaa agcgccgtca gtcgccaaag agttgttcgc acagggcatc
      841 ccaatcctgg gcatctgcta cggcatgcaa ttggtcacac atctttccgg cggggaggtc
      901 gccaaggcgt cgcatcggga atacggacgg gctgagctca cgatcgatga tcgatcagat
      961 ctcttcaaag gcgtcggcga gggcggctcg accattgtct ggatgtcgca cggtgaccgg
     1021 atcgagcgca tgccgccggg gtttcgctcg attgcgcata cgggaaattc tccgatcgcg
     1081 gctatgcgac gacacgacgg gaaaggccga atctattgcc tgcagtttca tcccgaggtg
     1141 gcacatacac cggaaggcgc tacggtcctg cgaaatttcg tgtacgacat ctgtgggtgc
     1201 cagccgtcat ggacgatgcg gtcctatgtc gataccgccg tggagcagat ccgcaaacag
     1261 gtcgggaagg atcaggtcat ttgcgcgtta agcggcggcg tggactcctc cgttgcggcg
     1321 gtattgacgc accgtgccat cggcgaccag cttacctgtg tattcgtcga taacggagtg
     1381 ctgcgagccg gagagcgcga tcaagtgcaa aagacctttg ccgcgaactt taaattaaat
     1441 ctccggattc tcgatcgaac aaacgagttc cttgccggtt tgaagactgt gatcgatccg
     1501 gagcgaaagc ggaagatcat cggccggctc ttcatcaaga actttgaagc ggaatcgaag
     1561 aaattgaagg gagtcaagta tctggtccaa gggacgctct atccggacgt gatcgagagt
     1621 gtcagtttta aggggccgtc cgcgacgatc aagacgcatc ataatgtcgg cggcttgccg
     1681 gcgcggatga aactgaaatt gatcgaacca ctgcgagagc tatttaagga tgaagtgcga
     1741 gtccttggga aggaattggg gattcccgac gacgtcatct ggcgtcagcc cttccccggt
     1801 cctggtctgg cgattcgcat tctcggctcg gtcaccaagg aacggctgac gatccttcgt
     1861 gaggcggaaa cgatcgtgga acaagaaatt cgagcggcag gattatatcg cgagatctgg
     1921 caggcgtttg ccgtgttgct gccgattcgt acggtcggcg tgatgggcga ccagcggaca
     1981 tatgaaaacg ttgtcgccat tcgtgcggtg accagcctcg acggcatgac cgctgactgg
     2041 gccaagattc cgaacgaggc tctaggcaag atgtcgaacc gaatcatcaa tgaagtgaag
     2101 ggtgtgaatc gggtcgtgta cgatatcagt tccaaacccc catccaccat tgaatgggaa
     2161 tagcagaacg tccgatggaa atccgactgc aaaaactgat tgccggaacg ggattggcct
     2221 cccgccgcaa ggctgaaacc ttgatcagtg caggacgggt cacggtcaat ggtcgggtgg
     2281 tgacggaact tgggacgaag gtcgatccaa tccgcgacca cgtgaaagtc gatggcaaac
     2341 acctcagctc ggcgcagccc tacgtctatc tcatgttgaa caagccgaag cacgtgatgt
     2401 cgacgttgga cgatccaggc ggccggccga ccgtgaaaga ttatctccgg ggcgtgagcg
     2461 ttcgggtatt tcccgtcggt cggttggact ttgacagcga agggctcatg ctgctcacga
     2521 acaacggtga tttggcgcag gctctcctgc atccccggta tcacgtgcca aagacctatc
     2581 tcattaaggt caagcgcgtg ctcacagatg aagagattcg aacgctggaa cgtggcgtga
     2641 aactcgacga tggcatgacc agcccggctc acgtgaagaa ggtgaagaag gtcgagcaaa
     2701 actcctggct ggaattgacg atccgcgaag ggcgcaaaca tcaagtgaag cgcatgttgg
     2761 atgcggtggg gcatccggtc ttgcggctca tacgagtgaa aatggggccg ctggtgttgg
     2821 gcgatttgga accaggagag tttcggtttt tagaggatcg tgaggcgaac gccctgcgcg
     2881 agcttgtcga gcaacggcag caatcggtgc aagagggaag attccctgca gagaccaaga
     2941 agcctgcgcg gcgagcggga tgggcacgtc cgaagaaggt gaacgtggca tgaagacccg
     3001 aacgcatcgg tgcggcgagc tgaataaggc acatgttggt caaaccgtcg ttctcaatgg
     3061 atgggtccag cgccaccgcg atcatgggat ggttatgttc atcgacctcc gcgaccgcac
     3121 gggcatcacg caggttgtgt tcaacgcgga gcgaaacgcc gctgtccatc aagctgccca
     3181 ttcgctgaga agcgaatacg tcgtgtccat caccgggcaa gtcatggcgc ggccggcaga
     3241 gtcgagaaat ccgaatcttc cgaccggaga tatcgaagtg tttgtggatg ccgttgaggt
     3301 gttaaatgag gccaagactc cgccgttcgt gatcgaggat gatgccgacg tcacagaagc
     3361 cgttcgtctc aagtaccggt accttgactt gcgccgcccc aaaatgcagc gtctcctcgc
     3421 gatgcggcat aatgtcacgc aagcggtccg cagtttcttg aatcgggaag ggtttctcga
     3481 aattgagacg ccgattctca cgaagagtac accggagggt gcgcgggact atctggtgcc
     3541 cagccgagtc aatccaggga tgtttttcgc gctcccgcag tcaccacagc tcttcaagca
     3601 agtcctgatg gtgagcgggg ttgatcggta ttaccaaatc gcccgttgtt tcagggacga
     3661 ggacctgcgg aatgatcgcc agccggagtt cacccaaatt gatcttgaga tgtcgtttgt
     3721 cgatcgcgat caggtgatga gtgtcatgga acagatgatc gtcacggtct tccgcgaagc
     3781 cggtggcgtc cagctgccga aaccgtttcc gcgcatgacc tatgccgagg cgatggggcg
     3841 gtacggctcg gacaagccgg atctgcggtt tgacatgccg cttcacgatg taacggcttt
     3901 tggcgcggcc agtgagttca aggtgtttcg ggaagctgcc acgaagggca aaatcgtcaa
     3961 ggcaatgatc gtgaaaggcg gcgggaccat cgcgaggagt cgaatcgatg cgctggggga
     4021 aaccgcgaag agtttcgggg cgaaggggtt ggcgtggctc aagatcacga cagacggcca
     4081 actcgattcg gtcattgcga agtttctcga tgccaaggca ttcttcgccg ctctgccaga
     4141 ggccaaacca ggcgacctcg tcctgttcgg tgcggacaaa tcggctatcg tgcacgatgt
     4201 cctgggccgg atcagactgt tgctcggtga ggaactgaac ctgatcgaca agacagcctg
     4261 gaagccggtc tgggtgacag aatttcctct gctggactat tcagaggaag agaagcgcta
     4321 tgtgttcatg cacaatccct ttgccgcgcc gatggacgag gacctcgcgc ttcttgatgc
     4381 agaacccttg aaagctcgtg ccaaggccta cgacatggtc ctgaacggca acgaagtcgg
     4441 cggagggagc atccggaatc accggagcga gatccagctc aagattctcg acttgctggg
     4501 gatcagcaaa gatcaggcac aggcgaaatt cggttttcta ctcgaggccc tcgactatgg
     4561 cgcgccgccg cacgggggca tcgcctttgg cctcgaccgc ctcatcatgc tgctcggcgg
     4621 tgccgactcc attcgtgacg tgatcgcgtt cccaaagact cagaaagcgc aatgtccgct
     4681 taccgacgca ccgtcctcgg tgggtccgga tcagctcaaa gagctccgaa tcaagctcga
     4741 tctggtggag tgaacgggcc gcacgtacac atcatggctg gcaatacctt cgggcgactc
     4801 ttcacggtga cctccttcgg tgaaagccac gggcctgcga tcggctgtgt ggtggatggg
     4861 tgcccgcctg ggatgatgct gtcggcggac gacgtgcaga aagatctcga tcggcggaag
     4921 ccgggcacgt cgcgacatgt gacgcagcgg caggaatccg atacggtgga aattctctcg
     4981 ggtgtgttcg aagggaaaac gaccgggacg cccattgctc tcttcattcg gaacgaagat
     5041 gcgcgcggcc gcgactacgg caatctcgtc gacaccttcc gccccgggca cgcagactat
     5101 acctattggc agaaatacgg catccgcgac catcgaggcg ggggacgggc ctccgcccgt
     5161 gaaacggcag tacgtgtcgc ggccgctgcc atcgcgagaa aatggctttc ggagaagcat
     5221 ggcgtcgtca ttcgcgggta tctgagtcag cttggaccga tcccgattcc gttcaagacc
     5281 tggagcgctg tcagtaccaa cccgtttttt gccgccgatc ccgacatcat tccaaaactc
     5341 gaagccttca tggatgattt gcgcaagtcc ggtgactctg tcggcgcggc gatcacaact
     5401 gtcgcggaac atgtgccggt cgggctcggc agtccggtgt atgcgaaatt ggatgcagat
     5461 ctcgcggcgg cgatgatgag catcaatgcc gtgaaaggcg tggaaatcgg ttcaggtttt
     5521 gcctcagtga cccaatgcgg gtcggaacat tgcgacgaat tgacgccgga gggatttctt
     5581 accaaccacg ccggcggcat tctcggcgga atttcaaccg gacagaacgt cgtggtcaca
     5641 atcggcatca agccaacctc gagtgtccgt ctcccgcgcc gatcgatcga taaacagggt
     5701 aatccggtca ccgtcgagac gaa
//
LOCUS       DATDNW010000136         8340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1106757, whole genome shotgun sequence.
ACCESSION   DATDNW010000136 DATDNW010000000
VERSION     DATDNW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106757"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..710)
                     /locus_tag="VI359_03400"
     CDS             complement(78..710)
                     /locus_tag="VI359_03400"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY6973332.1"
                     /translation="MAYRAKICGITNIEDALIAVAAGADALGFLLYRKSPRYVDPMTV
                     QRIVAELPPFVVPVGVFVNEEIKVVRDLMDTCGLALAQLHGEETAAYCRELSRPVLKA
                     LRLKDRSTFLALAEFQGRAGVRGFVLDAFSDQTYGGTGQVVDWGLASEAAQAATVILA
                     GGLTPENVGQALRAVQPYGVDVSSGVEVAPGKKDHEKVRAFLRAVRVVSP"
     gene            complement(732..1559)
                     /gene="trpC"
                     /locus_tag="VI359_03405"
     CDS             complement(732..1559)
                     /gene="trpC"
                     /locus_tag="VI359_03405"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY6973333.1"
                     /translation="MILDRILEHKKAELRHKQSRGYLAELTGRIKDLPRAMGFAVALD
                     ATKTVASPALIAEVKKASPSLGLLRPEYGEKFDHLEIARSYYAHGASALSVLTDKDFF
                     QGSLDHLREIKHAIPLPVLNKEFMIGEVQFYEARAHGADAVLLIVAALEREQLIDFHA
                     LTKELDMDTLFEVHHERELDTVLERIPDARLIGINNRDLKTFSTDLGVTLRLAKRVPE
                     DRLIVSESGIHKRDDVIRLLEAGVQAMLIGESLIRADDIGAKIQELRGANDRTPTTS"
     gene            complement(1648..2658)
                     /gene="trpD"
                     /locus_tag="VI359_03410"
     CDS             complement(1648..2658)
                     /gene="trpD"
                     /locus_tag="VI359_03410"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY6973334.1"
                     /translation="MIKDAIAKLADRISLTDKEAEEVMAEIMDGAAKPAQIAAYLMGL
                     RLKGETVDEIAGSVRAMRSRAIRIAVGDPNVVDTCGTGGDGAHTFNISTTTAFVVAGT
                     GLTVAKHGNTSVSSKSGSADVLAALGVKIDLSTERVTDCINEVGIGFLFAPLYHGAMK
                     HCAGPRHEIGIRTIFNILGPLTNPAGAAIQVLGVFDGKLTELVGKVLLQLGSRHCFVV
                     HGMDGLDEITLTDRTMVSEGKQGVLSSYVLDPQEFGLSRVRLKDLAGGHPPENAEITR
                     DILQGRKGPKRDIVCLNAAPALVAGRKAKSLQEGFRLAAQTIDSGAAAEKLERLIAYT
                     NK"
     gene            complement(2738..3301)
                     /locus_tag="VI359_03415"
     CDS             complement(2738..3301)
                     /locus_tag="VI359_03415"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY6973335.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRVYRNNKITLDEIEALHP
                     GRVVISPGPCTPKEAGISVDAIRRFGGAVPLLGVCLGHQSLAVAFGGEVVRAERLMHG
                     KTSWIKHDGKTIFKDLPNPFEATRYHSLIVKRSTLPSCFEISAETDEGEIMGIRHRSL
                     GIEGVQFHPESILTAVGKDLLRNFLKL"
     gene            complement(3309..4805)
                     /gene="trpE"
                     /locus_tag="VI359_03420"
     CDS             complement(3309..4805)
                     /gene="trpE"
                     /locus_tag="VI359_03420"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY6973336.1"
                     /translation="MRTPQYSLSLDQFRRYAHEGNLIPLYREILADYETPVSAFAKID
                     HGPSAYLLESIEGGEKWARYSFLGGGTPVVVYEEGGDLILLRGKVKKRVPSFGQPLER
                     LRELMQSYRPVTLPDLPRFVGGAVGYLGYDMVRTFEDLPTRRKDQGAAPDFAFLLTDT
                     LLIFDNVAQKIKVVANAHLKSTADRDIKRAYHEATERIERMIARLKRPLRRVRPHRRS
                     RPITFTSNMSKADFEKMVARTKEYICAGDIVQAVLSQRWETNVQTSPLQLYRALRVVN
                     PSPYMYYLRIAGVELVGSSPETLVRCEDGVISVRPIAGTRRRGVTVEEDQQLARRLLA
                     DEKERAEHVMLVDLGRNDVGRVAERGSVHVDSLMHVERYSHVMHIVSNVTGRLDPSKT
                     SFDVLRACFPAGTVSGAPKIRAMEIIDELEPTRRGPYAGAVGYFSFSGNMDMCINIRT
                     VVLKKHQAYIQAGAGIVADSNPEHEYEETCNKARAMMKAVELAEQGLE"
     gene            complement(4825..5475)
                     /locus_tag="VI359_03425"
     CDS             complement(4825..5475)
                     /locus_tag="VI359_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY6973337.1"
                     /translation="MKEEGGRMILNPKVVHRTVLVFLGTFVLSGCVLSEKYEAEKARS
                     LNFQRLLAQEEKRTSELDSEVKRTKRELSEYEARNRELTAQVQAVREQMSRVQEEAEA
                     MKEAALLERKAKTDVKRSSAAPARTTKPDANLEGTDFSGAFTGMPSDTKEATESASVD
                     TTPEVKTGGMVHVVKPGETLYRISRRYGVSVDKIKKWNKLPDEIIEVGQKLIVGQE"
     gene            5681..7708
                     /gene="ligA"
                     /locus_tag="VI359_03430"
     CDS             5681..7708
                     /gene="ligA"
                     /locus_tag="VI359_03430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY6973338.1"
                     /translation="MATSDIPSRITALRKAIQRHDYLYYVKDRPEISDSEYDRLFREL
                     TELEQANPDLVTPDSPTQRVGAPPLDELIKIPHEQPMLSLDSIIDSTEVIAFDQRMKR
                     ELHVPSVEYAAEPKFDGLSVELVYDHGLFMRGATRGDGITGEDVTVNLRTIRSLPLQL
                     QPHSDFPAHLVVRGEVYMRLDDFQALNRRMTEREGEAFANPRNAASGSLRQLDSNVTA
                     QRPLVVTCYEVMVSSESQLPTHWAELEALSRWGLPVPTHRQLCRSIDDVVEFHHNTEA
                     IRDTLSYEIDGVVVKVNHRDWQRRLGVKSRSPRWAIAYKFTPRKEVTVIQEIVVSVGR
                     TGTLTPIALLKPVEVGGVTISRATLHNADEVARKDIRMGDTVKVERAGDVIPAIAERV
                     PVDGERRAEPFAMPAHCPVCGSTVAREGAYYYCTGQTVCPAQLKGAIEHFASKAAMNI
                     EGLGHKTVAQFVDRGLVHSLADLYNLTKERLMALDGFAERSATLLLEAIERSKVVTLD
                     RFLMGLGIRQVGQHVAKVLAREFHTLPAIMAAKQECLQQVREIGPEISTSLFSFFQEE
                     HNRLVIERLLAQGVTISSTPQARTEGTAPLAGKTFVFTGGLDQLTRDEAAKLVEERGG
                     TISSTVSRKTSYVVAGREPGSKLDQAKKLGVSILSEKDFATLLGEAGSRPN"
     gene            complement(7701..>8340)
                     /locus_tag="VI359_03435"
     CDS             complement(7701..>8340)
                     /locus_tag="VI359_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY6973339.1"
                     /translation="NLHLKEAQELLYNSKYSRVPVYDGTLDNITGILYKTRALTELSK
                     GRFDVKLKDIANPALFVPAGKTADDLMKQFQQDKRHMAVVVNEFGGVMGIVTLEDLLE
                     EVVGEIMDETDITEELIKRLGKNQILVHGRTEVHKINEFLKVDLGDEAVTISGLIQEQ
                     LGRIPKVGDELKIGNCRILIHEADPRSIRSVQIVREERIPTAVETPNLDLVS"
BASE COUNT         1837 a         2385 c         2136 g         1982 t
ORIGIN      
        1 gaaccgccct ctcttgtcag gaactgcaac catagaattc tctgtgatga tataaaaaaa
       61 cagtatagac gggcctgcta cggagacaca acccgcaccg cgcggagaaa cgctcgaacc
      121 ttctcgtgat ctttctttcc tggagcgact tcaaccccgc tgctgacatc gacgccatac
      181 ggctgcaccg ctcgaagagc ctgaccgaca ttctctggtg tgaggcctcc cgcgagaatc
      241 actgtcgctg cttgcgccgc ctcactggct aagccccaat cgacgacctg accggttcct
      301 ccatacgtct gatcggagaa cgcgtcgagg acaaagccgc ggacccctgc cctcccttga
      361 aattctgcga gggcaaggaa ggtgctccga tccttcaatc gcagcgcttt cagcaccgga
      421 cgactcaatt cacggcaata cgccgcggtt tcctccccat ggagttgcgc gagtgccagg
      481 ccgcaggtat ccatgagatc gcgtacaacc ttgatttctt cgttgacgaa cactccaacc
      541 ggcaccacaa acggcggcaa ctcggcaacg atccgttgaa ccgtcattgg atcgacgtat
      601 cgagggctct tccgatagag aaggaagcca agcgcatcag cccctgccgc tacggcaatc
      661 aaggcatcct cgatattcgt aattccgcaa attttcgctc gatatgccac ggtggtttcg
      721 actttcaccc actatgacgt ggttggagtc cggtcattcg ctccacgaag ttcctggatc
      781 ttcgcgccaa tatcatcagc gcgaatcaag gattcaccaa tgagcatcgc ctggacaccg
      841 gcctcgagga gtctgatcac gtcgtctcgt ttgtgaatac cgctttcgct cacaatcaac
      901 ctatcctctg ggactcgttt ggccagccgc aaagtcacac caagatccgt tgaaaaggtc
      961 ttgaggtcac gattattgat tccgatcaac cgcgcatccg ggatccgttc aagaaccgta
     1021 tcaagctcgc gttcatggtg cacttcaaac agcgtgtcca tatccagttc tttggtcaaa
     1081 gcgtgaaaat cgatcagttg ttcccgctcg agcgcggcaa cgattaagag caccgcgtcg
     1141 gcaccgtgag cgcgagcttc gtaaaactgc acctctccaa tcatgaattc cttgttgagc
     1201 acgggaagcg gaatcgcatg tttgatttcg cggagatggt caaggctgcc ttgaaaaaaa
     1261 tccttatcgg ttaagaccga cagcgctgaa gcgccatgtg catagtacga tctggcgatt
     1321 tcaagatgat caaacttctc tccatactcc ggtcgcagaa gccctaaact tggggacgct
     1381 ttcttaacct cggcgatgag ggcagggcta gcaaccgttt tggtggcatc taatgcgacc
     1441 gcaaacccca ttgctcgagg aaggtccttg atccgaccgg tgagttcggc caagtaccca
     1501 cgactctgct tgtgtcgcaa ttcggctttc ttgtgttcga gaatacgatc caggatcata
     1561 ttcggttaag gctcccagcc agtagttgac gttctccaat ctgcaggcga cgttgtcggt
     1621 accccttgcc cagaccttta cactctccta tttattcgta taggcgatga gccgttcgag
     1681 tttttctgcg gcagcccctg agtcgatcgt ctgagcagcc aggcgaaacc cttcctgaag
     1741 cgactttgct ttgcgtccgg caaccagagc aggagccgca ttcagacaca cgatgtcccg
     1801 tttcggcccc tttcgccctt gtagaatatc gcgtgtaatc tccgcatttt ctggtggatg
     1861 cccgccagcg aggtccttca ggcgtacgcg tgacagcccg aactcctggg gatccagcac
     1921 gtaactcgag aggactccct gcttgccctc tgacaccatg gttctgtcgg tcaaggtaat
     1981 ctcgtcgagt ccatccatgc cgtgcacgac aaagcaatgc cgcgagccca gctgtaagag
     2041 aacctttccg accagttcgg tgagttttcc atcaaacact cccagtactt gaatggcagc
     2101 gccggctgga tttgtcagtg gcccgagaat gttgaatatc gtcctgattc caatctcgtg
     2161 ccgtggtcct gcgcaatgtt tcatagcgcc atgatagaga ggtgcaaaga ggaatccgat
     2221 cccgacctca ttgatgcagt cggtcacgcg ttcagtcgac aaatcaattt tgacgcccaa
     2281 agcggctaag acatcggcac tccccgattt cgaagagacc gacgtgttgc cgtgtttggc
     2341 aacagtcaga ccggtacctg caaccacaaa cgcggtcgtc gtcgagatgt tgaaggtatg
     2401 ggctccgtct ccgccagtgc cacaggtatc caccacattg ggatctccga ctgcgattcg
     2461 aattgcacgg gaccgcatcg ctcgcacgga accagcgatt tcgtcgacgg tttcaccctt
     2521 gagacgaagc cccatcagat acgcagcgat ctgggcaggc ttggctgctc catccatgat
     2581 ttcggccatc acttcttcgg cttctttgtc cgtaagagaa atgcggtcgg cgagtttggc
     2641 aatggcgtct ttgatcatat tcaacagcga tcagctagca gcgatcggct tcgtagtagc
     2701 tcatctgcct gaaggctgac cgacgattgc taaatggcta cagttttaag aagtttctca
     2761 gcagatcttt cccgactgcc gtcagaatcg attccggatg gaactgcacg ccttcaatgc
     2821 cgagcgatcg atggcgaatc cccatgattt ctccctcgtc cgtttcggcc gagatttcaa
     2881 aacaggacgg gagtgtcgaa cgctttacga tgagggaatg gtaccgtgtt gcttcgaagg
     2941 gattgggaag atctttaaag attgtcttcc cgtcatgctt aatccatgac gttttcccat
     3001 gcatgagacg ttcggcccgc acgacttctc ctccaaacgc aaccgccaac gactgatgtc
     3061 ccaggcatac gcccaataag ggaaccgctc ctccaaaccg cctaatcgcg tcgactgaga
     3121 tgccggcttc cttgggcgtg cagggacccg gtgaaatgac cacgcggccc ggatgtagcg
     3181 cctcaatctc atccagcgta attttgttat ttcgatagac gcggacatct tcgcccaact
     3241 cgccaaaata ttgcacaagg ttgtaggtaa aggagtcgta gttgtcgatc atcaaaagca
     3301 tgaccgtgtt actccagtcc ctgctccgcc aattcaacgg ctttcatcat ggcgcgggcc
     3361 ttgttgcatg tttcctcgta ctcatgttcg ggattggaat cggcgacgat cccggcaccg
     3421 gcctggatat aggcctgatg tttcttcaga acgactgtcc ggatgttgat acacatgtcc
     3481 atgttgcctg agaaactgaa atagccgacg gctccggcat agggcccgcg tctcgttggc
     3541 tcgagctcgt cgatgatttc catcgctcga atcttcggtg caccggacac agtcccggcg
     3601 ggaaagcatg cgcgcaaaac atcgaaggaa gttttagacg gatcaagccg tcccgtgacg
     3661 ttcgatacaa tgtgcatgac atgggagtac cgctccacat gcatgaggga gtcgacgtgt
     3721 acgctccctc gttcggccac gcgaccgacg tcgtttcggc ccaaatcaac gagcatgacg
     3781 tgttcggccc gctccttttc gtccgcaagc agccggcgcg cgagctgctg atcctcttcc
     3841 accgtgacac ctcgccgtcg tgtccctgca ataggcctga ctgaaatcac tccgtcttcg
     3901 caccggacta atgtttcagg agatgatccc accagttcca cccccgcgat tcgcaagtaa
     3961 tacatatatg gagaaggatt caccacacgc agcgcccggt aaagctgcag gggacttgtc
     4021 tggacattag tttcccatcg ctgggacagc acggcctgaa caatgtctcc cgcgcaaatg
     4081 tattccttgg tccgagcaac cattttctcg aagtcggctt tgctcatatt cgaggtaaac
     4141 gtgatcggtc ggctgcggcg gtgagggcgc acccgtcgga gtggccgttt gagtcgagcg
     4201 atcatccgtt cgatgcgttc tgtcgcttca tggtaggctc gtttgatatc tcgatcagcc
     4261 gtcgatttca gatgggcgtt ggctaccact ttaatctttt gggccacgtt gtcgaagatc
     4321 aacagagtat cggtcagcaa aaaagcaaaa tccggagcag caccctggtc ttttcgccga
     4381 gtcgggagat cctcaaaagt gcggaccatg tcatacccaa gataccccac agcacctccc
     4441 acaaatcggg gaagatccgg cagcgtaact gggcggtagc tttgcattaa ctctcgcaga
     4501 cgttcgagcg gctggccaaa actggggacg cgctttttca cttttccccg gaggaggatc
     4561 agatctcctc cctcttcgta cacgacgaca ggtgttcctc ctcctaagaa cgagtatcgc
     4621 gcccattttt ctccaccctc gatactctca agcaaatagg cagacgggcc atgatcgatc
     4681 ttggcgaaag ctgagaccgg cgtctcatag tccgccagaa tctcccgata aagggggatg
     4741 agattcccct catgggcgta acgacgaaac tggtctaggc tcaacgaata ctgtggcgtc
     4801 cgcacgtgaa tcgtcctatt gtcgttattc ctgaccgaca atgagctttt gcccaacctc
     4861 gatgatctca tcgggtaact tgttccactt cttgatctta tcgacactaa ccccatagcg
     4921 gcgactgatg cggtacagtg tttcccctgg cttgaccaca tggaccatac ctccggtctt
     4981 cacctccggc gttgtgtcca cagatgctga ctcagtcgcc tctttggtat ccgagggcat
     5041 gcccgtgaag gctcctgaaa agtcggtacc ttccagattt gcatcgggct tggttgttcg
     5101 tgctggggct gccgatgatc gtttcacatc ggtcttggct ttccgctcga gcaaagcggc
     5161 ttctttcatc gcttcagctt cttcttgcac ccggctcatc tgttccctca ccgcttggac
     5221 ctgagcggtt aattcgcgat tccgcgcctc gtactccgac aactccctct tcgttcgctt
     5281 gacttcgcta tcgagttcac tggtccgttt ttcttcctga gccaacagac gctggaagtt
     5341 gagactcctg gctttttccg cctcatattt ttcactgagt acgcatccac tcagcacgaa
     5401 agttcccaag aacaccagaa cggtgcggtg gaccactttg gggttcaaga tcattctgcc
     5461 tccctcctcc ttcatgaaga cctccttcta catttcctgg tttctgcccc taagcaacca
     5521 agagagcgca ccggctaccg gacaaagcag gcaccttgcc gaggattgga taggatagtg
     5581 gctaacgctg cgaaagtcaa agcgaatatg gctgtttcga tcgtttgacc cgcttcctac
     5641 ccctgtgcta cggtcgacaa gcgaccgagg ttccttcctc atggccactt ccgacattcc
     5701 ctcccgtatc accgccttgc gtaaggcaat ccagcggcat gattacctct actacgtgaa
     5761 ggaccgaccg gagatttccg actccgagta cgatcgccta tttcgagagc taactgaact
     5821 tgaacaggca aatccggacc tcgtaacccc cgactctccc acgcaacgtg tcggtgcccc
     5881 acctttggac gaactcatca aaataccaca cgaacaaccc atgttgagcc tggattcaat
     5941 cattgattca accgaagtca tcgcttttga tcagcgcatg aaacgagaac tgcatgtccc
     6001 ctcggttgag tacgccgctg aaccgaaatt tgatggcctg tctgttgaac tcgtatacga
     6061 tcacggtctg ttcatgcgag gggcgacacg aggcgatgga atcaccggcg aggatgtgac
     6121 cgtgaatctc cgaacgattc ggtcactccc gctccaactc caaccccact cggacttccc
     6181 cgcccatctg gttgtgcggg gagaggtcta catgcgactg gacgattttc aagcattgaa
     6241 ccgccgcatg accgagcgag aaggcgaggc gtttgctaat ccgcgcaatg cagcgtccgg
     6301 ttccttacga caattggatt cgaatgtgac agcacaacgg cccttagttg ttacttgtta
     6361 tgaagtgatg gtttcgtccg aatctcaatt gccgacacat tgggctgaat tggaagcgtt
     6421 gtcccgttgg gggctgcctg ttccgactca tcgacaactg tgccggtcta ttgatgatgt
     6481 cgtggagttc catcacaaca ctgaggcaat acgcgacact ctttcttatg aaatcgatgg
     6541 cgtagtggta aaggtaaacc accgagattg gcagcggcga ttgggcgtga agtcccgaag
     6601 tccacggtgg gccatcgcgt acaaattcac cccccgcaaa gaggtcacgg tgattcagga
     6661 aatagtggtg tcggtcggac gtactggaac tctcacaccc attgccttac tcaagccggt
     6721 agaagtcggt ggtgtgacta tcagtcgagc gacgcttcac aacgcggatg aagtcgcacg
     6781 gaaagacata cgaatgggcg acactgtaaa ggttgagcga gccggcgatg tgattccagc
     6841 gatcgcggaa cgcgtaccgg tcgatggtga gcggcgcgct gaaccatttg ccatgccggc
     6901 ccactgtccc gtttgcggat cgactgtggc acgggaaggg gcctattact actgcaccgg
     6961 ccagacagtc tgtccagccc agctcaaagg agcaattgag catttcgctt cgaaggctgc
     7021 gatgaacatc gaaggactag ggcataagac cgtggcgcaa ttcgtcgatc gaggtctagt
     7081 tcattcactg gctgacctct ataacctgac caaagagcgg ctgatggcac ttgacgggtt
     7141 tgccgaacga tcggccacgc ttctactcga ggcaattgaa cggagtaagg tcgttacgct
     7201 ggatcgcttc ctgatggggc ttggtatccg gcaagtcggc cagcatgtgg ccaaggtgct
     7261 ggcccgcgaa tttcatacct tgccagccat catggcggct aaacaggaat gcctgcagca
     7321 ggttcgtgag attggccctg aaatctccac aagccttttt tcattttttc aggaagaaca
     7381 caaccgtctc gtcatcgaac gcctgctcgc ccagggtgtc acgattagtt ctacaccgca
     7441 agcgcgaacg gaagggacgg cgccgttggc gggaaaaacc tttgtcttca cgggaggact
     7501 agatcaattg acccgtgacg aggccgctaa gttggtagaa gaacgaggtg gaaccatctc
     7561 ctcgacggtg agtcgaaaaa cctcctatgt ggtggcagga cgtgagccgg gatcaaaatt
     7621 ggatcaggcc aagaagctgg gagtatccat cctctcggaa aaagattttg cgaccttgct
     7681 cggagaggcc ggaagccgac ctaactgacc agatcgaggt tgggagtctc aacagcggtc
     7741 gggattcttt cttcccggac tatttgaacg ctgcgaatgg accgtgggtc tgcttcatgg
     7801 attagaatgc ggcaattgcc gatcttgagc tcatctccga ctttcggtat gcggcccaat
     7861 tgttcctgaa taagaccgct gatcgttacc gcttcgtctc ctagatccac tttcaagaat
     7921 tcattgattt tatggacttc ggttcttccg tgaaccagga tctgattttt cccaagacgc
     7981 ttgatgagtt cttccgtaat gtctgtctcg tccataatct cgccgaccac ctcttccagc
     8041 agatcttcga gcgttacgat tcccatgacg ccgccgaatt cgttcacaac taccgccatg
     8101 tggcgtttgt cctgctggaa ctgtttcatg aggtcatcgg cagttttccc tgccgggacg
     8161 aacaaggccg ggttggcgat gtcttttaat ttcacgtcaa accggccttt cgacaattcc
     8221 gtgagcgcac gcgtcttata gaggatcccg gtgatgttat ccaacgttcc gtcatacacg
     8281 ggaacgcgcg agtattttga attgtagagc agctcttggg cttccttgag gtggagattc
//
LOCUS       DATDNW010000137         6054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_85058,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000137 DATDNW010000000
VERSION     DATDNW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85058"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VI359_03440"
     CDS             complement(<1..661)
                     /locus_tag="VI359_03440"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973340.1"
                     /translation="MKYVILHADGLADRPRQELGGKTPLQVAATPHLDRVVKGGELGL
                     LSFLNGSGRHGSGLTGTAILGYDPKKYYQGPGPFEAASLEVNVTEYDVVYRCTMVTLK
                     AEPGAKGVSTDIKKLGPQVIMDDATAGLITTEEARELIDAANEQLGSETIQFYPGSGH
                     RHVMVWVNGKPRAICTDPQTLVGHPIAEGLPSGDGADILRKLMEASLHIFRDHPVNDE
                     RR"
     gene            complement(685..1743)
                     /gene="thrC"
                     /locus_tag="VI359_03445"
     CDS             complement(685..1743)
                     /gene="thrC"
                     /locus_tag="VI359_03445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY6973341.1"
                     /translation="MNRWRGVIEEYRKFLPVSEHTPIITLGEGNTPLIRATRLAKQIA
                     PGIDLYLKFEGANPTGSFKDRGMTMAISKAVEGGARAVMCASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHRATVIQIEGNFDQALSIVKDLSATQHIELVNSINP
                     FRIEGQKTAAMEVCDQLGDAPSVHVLPVGNAGNITAYWKGYQEYRAANQITKVPRMMG
                     FQAAGAAPIVLGHVVDNPQTVATAIRIGNPASWQLALNAVKESSGAIDMVTDDEILQA
                     YASVAATEGIFCEPASAASVAGVTKLNQAGALREGETVVCTLTGHGLKDADTAISVSV
                     QPKTVKATREDVARLLKV"
     gene            complement(1748..3061)
                     /locus_tag="VI359_03450"
     CDS             complement(1748..3061)
                     /locus_tag="VI359_03450"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY6973342.1"
                     /translation="MKSSIGVGLIGFGTVGSGVAKILLENAGLIQRRVGVPVRLIRIA
                     DLDIIRDRGLRLPDGMLTTDAKQILTDPAIDIVIELIGGYDTAKRIILEAMAAGKQVV
                     TANKALLALHGEDIFAAAVRHGVDLGFEASVGGGIPVIHAMTEGLAANAIQSIYGIIN
                     GTSNYILSRMTNEKQGFEAVLAEAQRAGYAEADPTFDVAGIDSAHKLAILANLAFGTP
                     INFKDLYTEGITHITQHDIAYAWELGYTIKLLGIAKLVDGEVEARVHPTMIASSLPIA
                     KVEGVYNAIQLVGDAVGDVMLYGRGAGDLPAGSAVVSDVISLARNLLKGAVGRIPPAS
                     FQQNQRRPLRMRPMDEISSLYYLRFMVVDRPGVLAQIAGVLGQFGISISSMLQQGRSA
                     GQTVSIVIKTHMAKERDVQTALREINRMTFISAPAILIRVEGKDE"
     gene            complement(3058..4254)
                     /gene="alaC"
                     /locus_tag="VI359_03455"
     CDS             complement(3058..4254)
                     /gene="alaC"
                     /locus_tag="VI359_03455"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEY6973343.1"
                     /translation="MGIGDGFYRIKRLPPYVFAQVQSLKLDARHRGEDIIDFGMGNPD
                     QPTPTHIVDKMIEAARKGKNHRYSASRGITKLRHAIAGWYKRNYDVDLDPETETIVTI
                     GSKEGLAHLSLAMIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRPDSDFFDDLQR
                     VYRQTFPRPKILVINFPHNPTTAVVDLEFFKKIVAFAKEHQVIVIHDLAYADIVFDGY
                     RAPSFLQIPGAKDIGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKIKSYLDYGIFQ
                     PLQIASVIALNGPQDCVKETVLRYQKRRDVLVSGLNRIGWYVAKPLATMFVWARIPLP
                     YRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEYVRFALVENEHRTRQAVRGIRKALK
                     LEGIDE"
     gene            4539..5252
                     /locus_tag="VI359_03460"
     CDS             4539..5252
                     /locus_tag="VI359_03460"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY6973344.1"
                     /translation="MPRLGVNIDHVATLRQARGGNDPDPLAAAVLVELAGADGIVVHL
                     REDRRHIQDKDLQLLRELIRTKLDLEMAADEAMAKIALMVKPDMVTLVPEHRQELTTE
                     GGLDVSGHKDRISQVVKMLHDGDIPVSLFIEPDLAQIKAAHKISADFVELHTGRYANA
                     KRSKEADAEFEAISQSSKLAYKLGIGVNAGHGLNYRNVKRLTMIPEIVEYNIGHSIIA
                     RAVLVGLDQAVREMKTLIG"
     gene            5398..>6054
                     /locus_tag="VI359_03465"
     CDS             5398..>6054
                     /locus_tag="VI359_03465"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY6973345.1"
                     /translation="MKLVTAAQMQTLDRRTITEAHVPSSTLMERAGEGVVTHLEKQYG
                     PSRGKTVAIFCGKGNNGGDGLVVARLLRRRHALVQVFMLAPLADLSRDAAVMYRRFVR
                     TVGRSAVKPFRSADQVRDLVTSSDFVIDALLGTGITSEITGPYREAIDLINEAHRPVI
                     AVDVPSGIHADNGAVMGRAVRASMTVTFGLPKLGLVVGPGIDHAGIVQVVDIGIPPAY
                     T"
BASE COUNT         1285 a         1807 c         1612 g         1350 t
ORIGIN      
        1 gcctccgttc gtcattgacc gggtggtcac ggaagatgtg cagggaggcc tccatgagct
       61 tccgcaaaat atcggctcca tctcctgatg ggaggccctc ggcaatcggg tgaccaacca
      121 gcgtctgagg atcggtacag atcgcccttg gcttaccgtt gacccaaacc atgacatggc
      181 gatgtcccga tcctggatag aactggatcg tctccgatcc caattgctca ttggcggcat
      241 cgatcagctc tcgcgcttcc tccgtggtga tgagtccggc ggtcgcatca tccatgatca
      301 cctgaggccc gagttttttg atatcggtcg agactccctt ggctcctggc tcagccttga
      361 gtgtgaccat cgtgcaacgg tacaccacat cgtactcggt gacgttaacc tccaggctgg
      421 cggcttcgaa gggtccagga ccctgataat atttcttagg atcgtacccg agaatggctg
      481 tacccgtgag tccagagcca tggcggccac tgccgttcaa aaacgacagg agccccagtt
      541 ctccaccttt caccacacga tccagatggg gagtcgccgc tacttggaga ggagtctttc
      601 cacccagttc ctgccgaggg cggtcggcga gtccatcggc atgcaggatc acatacttca
      661 tcgacagggc gctcgcatca atacctagac tttcagcaga cgggcgacat cctcccgcgt
      721 cgccttcacg gttttcggtt ggacggatac actgatggcc gtatcagcat ccttcaaccc
      781 atggccggtc aacgtacaca caactgtctc tccttcacga agtgcgccgg cttgattcaa
      841 cttcgtcact cccgcgactg atgctgccga tgccggttcg cagaagatcc cttcggtcgc
      901 tgccacgctc gcataggcct gcaaaatttc atcgtccgtg accatgtcga tggcgccgga
      961 cgactctttc acagcattca gcgccaattg ccaactggcc ggattgccga tccggatggc
     1021 cgtcgccacg gtttgtggat tatccaccac gtgtccgagc acgatcggtg ccgctccagc
     1081 cgcttgaaac cccatcatcc tcggcacctt ggtgatctga tttgcggcgc gatactcctg
     1141 atagcccttc cagtaggccg tgatgttccc ggcattgcca accggaagta catggacgct
     1201 cggcgcgtcg ccaagttgat cgcagacctc catggctgcg gtcttctgac cctcgattcg
     1261 aaagggattg atcgaattga ccagctcgat gtgttgcgtc gcagagagat ctttgacgat
     1321 cgagagcgct tgatcgaaat taccttcaat ttggatcacc gttgcgcgat gcatcatggc
     1381 ctgggagagc tttcccatcg caatcttgcc tgcgggaatc aagacgtaca cggccagccc
     1441 ggcccgagct ccataggcgg cagcagacgc agacgtattc cctgtcgacg cgcacatcac
     1501 agcccgcgct ccgccttcaa ccgctttcga gatcgccatc gtcatgccgc ggtctttgaa
     1561 ggacccggtc ggattagcgc cctcgaattt gagatagaga tcgatgcctg gagcaatctg
     1621 tttcgctaat cgtgtggctc tgataagagg cgtgttgcct tcgcctaacg taatgatggg
     1681 cgtatgttcc gaaaccggga gaaacttccg atactcttcg atcaccccgc gccagcgatt
     1741 cattatgtta ttcgtccttg ccttcaaccc tgatcagaat agcaggtgcg gagataaagg
     1801 tcatgcgatt gatctcccgt agcgctgtct ggacatcacg ttccttggcc atgtgggttt
     1861 tgatgacaat cgagaccgtt tgcccggcgc ttcgcccctg ttgtagcatt gatgaaatgc
     1921 tgataccgaa ctggccgagc actcccgcga tctgcgccaa gacccccgga cgatccacaa
     1981 ccatgaaccg gagatagtag agggagctaa tctcgtccat gggacgcatc cttaatggac
     2041 gccgctgatt ctgctggaac gacgcaggtg gaatacgccc gacagcaccc ttcaggagat
     2101 tgcgcgcgag gctgatgaca tcgctcacga ctgcacttcc ggcgggcaga tcgccggcgc
     2161 ctctgccgta caacatcacg tcgccgaccg cgtcgccaac aagctgaatc gcgttgtaaa
     2221 ccccttccac tttggcaatc ggcagggacg aggcgatcat ggtcggatgg acacgcgctt
     2281 cgacctcgcc gtcgacaagc tttgcgatgc ctaggagctt aattgtgtac ccgagttccc
     2341 aggcataggc gatgtcatgt tgtgtgatgt gtgtgatccc ttccgtatag agatctttga
     2401 agttgatcgg tgtgccgaat gccaggttgg ccaggatagc cagcttatgt gccgaatcga
     2461 tacctgcgac gtcaaaggtc ggatcggcct ccgcatagcc ggcccgttgc gcctctgcca
     2521 agacagcctc aaatccctgc ttctcgttgg tcatgcgaga aagaatgtag ttcgatgtcc
     2581 cgttgatgat tccgtaaata gactggatcg cattggccgc cagcccctca gtcatcgcat
     2641 ggatgacggg aatccctccc ccgacactgg cttcaaagcc gaggtccacc ccgtggcgca
     2701 ccgctgccgc gaagatgtcc tctccatgga gcgcaagcaa tgccttgttc gccgtcacta
     2761 cttgcttccc cgcggccata gcctccagaa ttatccgctt ggctgtgtca tagccgccga
     2821 ttaattcaat cacgatgtcg atggcaggat cggtgaggat ctgtttcgca tcggtggtca
     2881 acattccgtc tggtagacga agtcctcgat cacgaatgat atccagatcc gcaatccgga
     2941 taagccgcac aggtacaccg acgcgacgtt gaataagtcc ggcattctcc aacagaatct
     3001 tggcgacgcc acttccaacc gtgccaaacc cgatgagccc aacgcctatg cttgacttca
     3061 ttcgtcgatc ccttcgagtt tgagggcttt gcggattccg cggaccgcct gcctggtgcg
     3121 gtgttcattc tcgaccagtg caaaccgcac atactcatcg cccccctcac cgaacccgat
     3181 gccgggggag acggccactt tcgcctcgcg caacaacaat ttagaaaact ccagcgaccc
     3241 catgtgccga tagggaagcg gaatcctggc ccacacgaac attgtcgcca gcggtttcgc
     3301 aacataccac ccgattcggt tcaagccgct gaccaacaca tcccgtcgtt tttgataccg
     3361 gagaacggtt tctttgacac aatcctgcgg cccattcaag gcgataacgc tggcgatctg
     3421 cagcggttga aaaatgccgt aatccaggta gctcttgatc ttcgccagcg cgccaaccac
     3481 ctcccggttc ccgacacaga atcccacacg ccaccccggc atattgtaag ccttggagag
     3541 ggtatagaac tccacgccga tgtccttggc cccaggaatc tgcaggaagc ttggcgccct
     3601 atatccgtcg aacacgatat ccgcataggc caggtcgtgg attacgatca cctgatgctc
     3661 tttggcaaat gcgacgatct tcttgaaaaa ttccagatcg acgaccgcgg tggtcggatt
     3721 gtgcgggaaa ttgatgacca gaatcttggg ccgggggaat gtctgccgat agacacgctg
     3781 gagatcatca aagaagtcac tatccgggcg cagctcaatg ccgcggactt cccctcccgc
     3841 aatgataaaa ctatacatgt ggatgggata cgtgggcgta ggagtcaaga cgacatcgcc
     3901 gggcccgatc attgccaacg ataggtgcgc caacccttcc ttggagccga tagtcacgat
     3961 ggtttcggtc tcgggatcga ggtcaacgtc gtagttccgt ttgtaccatc ccgcgatcgc
     4021 atgccgaagt ttggtgatcc cgcgggaggc cgagtagcgg tgatttttgc cctttcgcgc
     4081 cgcctcgatc atcttgtcga cgatgtgggt gggtgtcggt tgatcgggat tccccatccc
     4141 gaaatcaata atgtcttcgc cgcgatgccg ggcatcgagc ttcaggctct gcacctgagc
     4201 gaagacatac ggcggcagcc gcttaattcg gtaaaacccg tctccgattc ccatcgttgt
     4261 cgcgcatccc cgcccgatcg ggatctgaca gtaagaaaca cccgtccttc tacagcggca
     4321 tattctaatt gcgtagaacc ggttgagtca aattatgttg tgattctgtg gggttccgca
     4381 cgcggtccaa tctgtagtcc gttccttgat ttgatgcctg tgacctgtcc tctccggtcc
     4441 ttgccgaccc agaatatttc tgttacaaca gcagcgcctt tcacatttct cattcacgtc
     4501 agtatctcct gggtttgcac tcctcagaag gaggctccgt gccacggctt ggcgtcaaca
     4561 tcgatcatgt cgcgaccttg cggcaagccc gaggtggaaa cgatccggac cccctggcgg
     4621 cagcggtgct ggtggaattg gccggcgccg atgggattgt ggtccatctt cgagaagacc
     4681 gtcgtcacat ccaggacaaa gatctccaac ttcttcgcga gttaattcga accaagctcg
     4741 acctggaaat ggctgccgac gaagcgatgg cgaaaatcgc gctgatggtc aaaccagaca
     4801 tggtcacact tgtccctgaa caccgtcagg aactcactac cgaaggtggg cttgacgtat
     4861 ccggtcacaa agatcgcatt agccaggtcg tgaagatgct tcacgacggc gacattccag
     4921 tcagtctatt catcgaacca gatcttgcgc agatcaaggc ggcccataag atatccgccg
     4981 atttcgtcga gttgcacacc ggtcgatatg ccaatgcgaa acggtctaaa gaggctgatg
     5041 ccgaattcga ggccattagc caatcgtcga agttggcgta taaattgggc atcggagtga
     5101 acgctggaca tggcttgaac taccgtaacg tcaagcggtt aacaatgatc cccgaaatcg
     5161 tggagtacaa catcggccac agcattatcg caagagccgt gctcgtcggc ctcgatcaag
     5221 ccgtccgtga aatgaagacg ctgatcggct gagttttacg acaacgaggc gccaccgtct
     5281 gatccgaaca ccagtcagtc gtcctgtcat gacacgactg tgtatgaagg atttctttct
     5341 cttctcctca tcaactcaat gcaccacgcc atttccgtac aggatgatga gccgctcatg
     5401 aaactggtca ctgcggcaca gatgcagacc ctcgatcgtc gaacgatcac cgaagcccat
     5461 gtgccaagtt ctactctgat ggagcgggcc ggtgaaggag tcgtaacgca tctcgaaaaa
     5521 cagtacggac cctctcgcgg gaaaaccgtc gcgattttct gcggcaaggg taataatgga
     5581 ggcgatggac tcgttgttgc gcgtctgctg cgccgccggc acgcactggt gcaagtgttc
     5641 atgctggccc ctcttgccga tctgagccgc gatgcggcag tcatgtatcg tcgattcgtc
     5701 cggacagtag ggcgctcggc tgtaaaacca tttcgctctg cggaccaggt tcgcgactta
     5761 gtgacctcca gcgacttcgt catcgacgcc cttctcggaa ccggaattac ctcagagata
     5821 acagggccgt atcgcgaagc catcgacctc atcaacgagg cccatcgacc agtcattgcc
     5881 gtcgacgttc cgtccggcat ccatgccgat aacggggcgg tcatggggcg agccgttcgc
     5941 gcctcgatga ccgtcacatt cggcctgccg aagcttggtc tggttgtggg ccccggtata
     6001 gaccacgctg gcatcgtcca ggtcgtcgac attggcattc cgccggccta tacg
//
LOCUS       DATDNW010000138         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_354107, whole genome shotgun sequence.
ACCESSION   DATDNW010000138 DATDNW010000000
VERSION     DATDNW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354107"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VI359_03470"
     CDS             <1..265
                     /locus_tag="VI359_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6973346.1"
                     /translation="VIERAIALTPHPIITPEDLPETIRNATAQDQARARGWATLEELE
                     KDYILRVLEAHRQDHGRAAAVLGIHRKTLLRKLRQYGQNQLGH"
     gene            691..1074
                     /locus_tag="VI359_03475"
     CDS             691..1074
                     /locus_tag="VI359_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973347.1"
                     /translation="MMERKSKTVLVVEDDSDLRYVLSVRLVSAGYTVYGAANGVEALD
                     QMEKHQVDVVLTDYHMPKMNGLELLANSRVKWPGIPVVVVSGDGDDMAHEATDRGAFA
                     WLRKGNGFTTLLEILASAMQQSVHA"
     gene            1110..1634
                     /locus_tag="VI359_03480"
     CDS             1110..1634
                     /locus_tag="VI359_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973348.1"
                     /translation="MKHRRSGPLSRANRWWPIRGLILLMALNGCDPGGQSVRPWLPEN
                     GRFIDMWNTYTHCYRSEDLDAVHLDARRLNHAAGAINAAADPILPPFIDPAHKELPVR
                     ASVDPAAMAAACTLRAGQIAQGTGHPHLAQEMFLIVITNFPQPPYRYYVAQAQRGLDL
                     LDVPNRSTFSSPPM"
     gene            1647..2030
                     /locus_tag="VI359_03485"
     CDS             1647..2030
                     /locus_tag="VI359_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973349.1"
                     /translation="MKSLRRLAMTPLVILAVVTLSHATDPMLNNVSETKTVSETIVRG
                     DVLMTEGEFYIVKDVTGHEVRLHVNKDTKADVKVKVGDKIEARVTPEGHATSLTLHIP
                     QNGSAPTIPGMDSTPPQRSPEKLVQ"
     gene            2105..2395
                     /locus_tag="VI359_03490"
     CDS             2105..2395
                     /locus_tag="VI359_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973350.1"
                     /translation="MTRTIVASLFTAMIGFSPIALAQTPSSPTTPPASAEKEQRSNKG
                     GQLRGLDRADQAAGEHGQQGRDRARDAQLNRPNFSERTGPPDHPVRPNRSGR"
BASE COUNT          614 a          715 c          686 g          520 t
ORIGIN      
        1 cgtgatcgag cgtgccatcg ccctgacccc ccaccccatc atcactcctg aagacctacc
       61 ggagacgatc cggaatgcga ctgcgcaaga ccaggcccgg gctcgcgggt gggccaccct
      121 tgaggaattg gaaaaggact acattctgcg cgtgttggag gctcatcgtc aggaccacgg
      181 tcgggcagcc gcagtgctcg gcatccaccg gaagacactc cttcgcaagc tccgtcagta
      241 cggtcaaaac caattaggtc attgatcagc ctgttctgtt cctcggcaag acaacttgtc
      301 cttacaaact gaaagcccgc ataccacctc gaatttcgac acatgtcatc cgatctatgc
      361 gacaacttgt ctccctgaag gagctatcct tcccagttca atgaagtagt cctgcaatcc
      421 tccatcagct tgattttgtg ttgaatcttt ggcgagcggg tcaattcatg ctcgtcggtc
      481 catcctttgc tctcttattc catcgtcata tttcatgctg cctgtggtct tggacagagc
      541 atggaaagac ttatcgatgg ttgtacgttg caagaaacct cacgatggcc ggatgcacgc
      601 gtctccatcg aaagaaacgt gaggaccgaa gatcgaatgt attcatcggt caggtatttc
      661 tcccatcgat ggagcaaggg aggtcaatac atgatggaac gcaagagcaa aacagtgttg
      721 gtcgtcgagg atgatagcga cttgcgctac gtcctttcgg tcagactcgt cagcgcaggg
      781 tacaccgtct acggagcggc aaacggagta gaagccttgg accaaatgga aaagcaccag
      841 gtcgatgtcg tcctgaccga ctaccatatg ccgaaaatga atgggttgga actcctcgcg
      901 aacagccgcg tgaagtggcc tgggattcca gttgtcgtcg tctctggaga tggtgacgac
      961 atggcacatg aggcgacgga ccggggagcc ttcgcatggc tccgcaaggg aaatggattc
     1021 acaacgctgt tggaaattct tgcctcagcg atgcaacagt cagtccatgc gtgaacggcg
     1081 gcgcgtgcat gccaggggtg gcgtacacga tgaagcatag aaggagtggt cctctgtctc
     1141 gagcgaatcg ttggtggccc atacgcggcc tgatcctcct gatggcgctc aatggatgcg
     1201 accctggcgg gcaatccgta aggccctggc tgccagaaaa cgggcggttc atcgatatgt
     1261 ggaacaccta tacccactgc taccgaagcg aagatcttga tgcggtgcat ctggatgctc
     1321 gccgattaaa ccatgccgca ggcgcgatca acgctgcagc agatccaata ctcccgccat
     1381 ttattgaccc ggctcacaag gaactgcccg ttcgcgcatc ggtagatcct gctgcgatgg
     1441 ctgctgcctg tacccttcgt gccggtcaga tcgcgcaggg gaccggacat cctcatctag
     1501 cacaagagat gttcctgata gtcatcacaa actttcccca accaccctat cgctattatg
     1561 tggctcaagc ccaacgaggt ctcgatctcc tggacgtccc gaaccgctct actttcagca
     1621 gtccccccat gtgaggaggt atcgacatga agtcgttacg gcgactggcc atgaccccgc
     1681 tcgtcatcct tgcagtagta accctcagcc atgcgaccga tcccatgctg aacaacgttt
     1741 ccgagacgaa gactgtatca gaaacaatcg tcagaggaga tgtgttgatg acggaaggtg
     1801 agttctacat tgtaaaggac gtcaccggtc atgaggtacg gttgcacgtg aacaaggaca
     1861 ccaaggcgga cgtgaaggtg aaggttggag acaagattga ggcgcgggtg acaccagaag
     1921 ggcacgccac gtctttgacg ttgcatattc cacaaaacgg gtcggcgccg accatacccg
     1981 gcatggactc gacgcccccg caacgatccc ccgagaaact tgttcaataa gattcaatcg
     2041 aatcatttta tgctggcagc ggatacggaa ccatcacacg gatctcaacg ggaggtacat
     2101 gaacatgaca cggacaatag tggcaagtct attcaccgcc atgatcggct tcagccccat
     2161 cgctctggcg caaactccat cgagccccac gaccccaccg gcttcagccg agaaagagca
     2221 gcgaagcaac aaaggtggcc aactgcgggg ccttgatcgc gcagatcagg cggctggtga
     2281 acatggtcag caggggcgtg acagggcgcg ggatgcacag ctcaatcggc cgaacttttc
     2341 cgaacggaca ggccctcctg accatccggt gagacctaac cgatctggcc gctagccgtt
     2401 cacagattcg cgcgccgacg aaacaattca cctcctttcg tccggcgcga tgcttgcgcg
     2461 agagaaaacc atctaaagag gaaaagctat atgcgtagaa tggtccaaac gtttgtcttg
     2521 aaacaggcgg tgata
//
LOCUS       DATDNW010000139         5209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_698690, whole genome shotgun sequence.
ACCESSION   DATDNW010000139 DATDNW010000000
VERSION     DATDNW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698690"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VI359_03495"
     CDS             <1..465
                     /locus_tag="VI359_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY6973351.1"
                     /translation="SRGLLGVRLHADGSGHGFIRIPAGGSRTPTTQETVKSKGHTLRM
                     KGAPLFRQAVRRLEEAVRDVVKEFGIDLQEVAQLILHQANGRILEQVAKRLRIPAERI
                     CSVIEHYGNTSSASLPIALDYAVRTGSIKPNDLVVLGSFGGGLTWATGIVRW"
     gene            553..1173
                     /locus_tag="VI359_03500"
     CDS             553..1173
                     /locus_tag="VI359_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY6973352.1"
                     /translation="MLFGLIPKEEAFFELFKKAAHNMIEGSRLLKEMMEHFQDPVQQA
                     KRIKDVEHVGDGITHDIALRLNQTFITPIDREDIHDLASALDDILDVTEAIADRFVLY
                     KVAKPTDMAIKLANILYQASAAVGRGVDRLGMGHPQVNECSVQVNSLENEADRVSRDA
                     ISALFEKENDPMVVLKLKEIYEGFEAGTDRCEDVANILERIALKHH"
     gene            1336..2328
                     /locus_tag="VI359_03505"
     CDS             1336..2328
                     /locus_tag="VI359_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY6973353.1"
                     /translation="MPDLSGMLLLVVLLALLFDFSNGWHDSANAIATVVSTRVVSPFV
                     AVMAAGVLNIAGAFMSTAVAKMVGSGIVDPTSVTQQMVAAALAGAILWNLLTLLLGLP
                     TSSSHALIGGLVGAAVTHGGWETVKLTGLRSVLEAMVLSPFCGFAMGLILMVLISWTC
                     FRIARSVATRFFKRLQLLSACFMAFSHGANDAQKAMGIITLALLSAGQIQTAEVPGWV
                     IGACAIAMGLGTAVGGWQIVRTLGMRIVKLEPVHGFAAETGAAAVLMVTAHIGLPVST
                     THTITSSVMGVGAIKRLSAVRWGVTKRILYAWLFTLPGAALLSCVIYLLGTKLS"
     gene            complement(2301..4166)
                     /locus_tag="VI359_03510"
     CDS             complement(2301..4166)
                     /locus_tag="VI359_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973354.1"
                     /translation="MTLPIRWKVTLATLVVLACGLIIAGLLAAQSIKQQEIAQSSQTL
                     EVRTQLVAHQLRPLLLGPNPLPPTTRLQATLRDLSSLALVRITLVTMDGSVLADSAVP
                     DGELAAVENHRTRPEIQSAIASGFGTDLRTSHTTGERTLYRAVGLKRDGDSTYSIFLR
                     LGLPMTMLEQELSKLQHNLTLAFGSAFLIAVALSVWLARGLTKPLSDMAKAASQLAAG
                     DHGVHIQTSAQDEVGLLGDALNHMTAELRVKIEELSEDRSQLLAMLTSMVEGVMVLDY
                     RGRVLQVNPALERMFDISRSEARGRRCADVFQHQDLNQLVSNVLASRNGQESEIILSP
                     SGRCLHVEASIAGGEQDNEACAVLVLHDITELRRLENIRKDFVANVSHELRTPLTSIK
                     GYVEALIDGGKNDPETSARFLDIILKQSDRLNLILDDLLQLSKIESGQVLFKRDPLHL
                     STVIERTLAMLKPLADKKGHRLTSWVAPDLPLVAGDEERLVQVLMNLIDNAIKYTPEH
                     GTVTVAAHRATSGPSSASSTSDQIDLTVTDTGMGIPERDRPRVFERFYRVDKARSREL
                     GGTGLGLAIVKHIVEGHGGQVWVEANIPTGSRFVVRLPAYHVVSSDTQLSLVPSR"
     gene            complement(4163..4873)
                     /locus_tag="VI359_03515"
     CDS             complement(4163..4873)
                     /locus_tag="VI359_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973355.1"
                     /translation="MSPAVSKKILIVEDEQDILQLVKLYLEKEGFRTVTAATGSEGLK
                     QVKQEKPDLIILDLMLPELDGLEVCKRLRSAPETAMLPIIMLTAKAEESDTVIGLELG
                     ADDYVTKPFSPKALMARVKALFRRLDRKPDERLTRYQYGRLVMDLARHEVTLNNTDIP
                     LTAKEFGLLEHLLRNPGRVLTREVLLNTVWGYDYYGTTRTVDVHIRRLKQKIPFLNEA
                     ITSVKSIGYKLKEADRTA"
     gene            5052..>5209
                     /gene="ispH"
                     /locus_tag="VI359_03520"
                     /gene_synonym="lytB"
     CDS             5052..>5209
                     /gene="ispH"
                     /locus_tag="VI359_03520"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY6973356.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIFVRHEIVHSRH
                     VVNSLRQKG"
BASE COUNT         1042 a         1395 c         1630 g         1142 t
ORIGIN      
        1 agccggggcc ttttgggtgt acgtctccat gccgacggat caggtcatgg attcattcgc
       61 attcctgcag gagggtcccg aaccccgacg acacaggaga cggtgaaatc aaaggggcac
      121 acgttgcgga tgaaaggggc cccgctcttt cgtcaggcag tccggcggct ggaagaagcg
      181 gtgcgggacg tagtgaagga gttcgggatc gaccttcaag aggtggcgca gctgatcctt
      241 catcaggcca atgggcggat tctcgagcaa gttgcgaagc gtctaagaat tccggcggag
      301 cgaatctgtt ccgtgatcga acattatggt aatacgtctt cggcttcgct ccccattgct
      361 cttgactatg cggtccggac tgggtcgatc aaacccaacg atcttgtcgt gctgggcagt
      421 tttggcggcg gactcacctg ggcgacgggc atcgtccggt ggtgaacaat gtacaagccg
      481 gtagccgttt cgtaatctca atgtaacagg aaccatggaa ggtgactacc gcacgatagg
      541 agggtcgact cgatgctgtt tgggttgatt cccaaggaag aagcgttctt cgaactgttt
      601 aagaaggcgg cacacaatat gatcgagggg agccggcttc tgaaagagat gatggaacac
      661 ttccaagatc cggtccaaca agccaaacga atcaaagacg tggaacatgt cggggacggc
      721 atcacccacg atatcgcgtt acggctgaac cagaccttta tcacaccgat cgatcgcgag
      781 gatattcacg acctcgccag cgcgttggac gatattctcg atgtgaccga ggcgatcgcc
      841 gaccgcttcg tgctctacaa ggtggcaaaa ccgaccgata tggcgatcaa gctggcaaac
      901 atcctgtatc aagcctctgc cgcggtcggc cggggagtgg atcgtttggg gatgggccat
      961 ccccaagtga atgaatgcag tgttcaggtg aacagcttgg agaacgaagc agaccgagtc
     1021 tctcgtgacg cgatttcggc gttattcgaa aaagaaaacg atccgatggt ggtgctcaag
     1081 ttaaaagaga tctatgaagg cttcgaagca gggaccgatc gatgtgagga cgtggccaac
     1141 attcttgagc gcatcgcctt aaaacaccac taaatgaact cggcaagagg taaggcgtga
     1201 gaggccggtg gcccatcaca agggatcaga ctgtggtgct tgaacgatcg gcaactttat
     1261 tggccgtctt ttctctcata gggcgtccgc acgtctggcc gctagcttcc ctgcctcttc
     1321 tctacaaggt gtcgcatgcc tgatttgagt gggatgctcc ttctcgtcgt cctgctggcg
     1381 cttctgttcg atttttcgaa cggctggcat gacagtgcga acgcgatcgc gaccgtggtc
     1441 tccacgcgag tggtcagccc gtttgtggcc gtcatggcgg cgggagtgct caacatcgcc
     1501 ggggcgttca tgtcgacggc ggtggcgaag atggtcggat cggggatcgt cgatccgacc
     1561 tcagtgacac aacagatggt tgcggcagcg ctcgccgggg cgattctctg gaatctgctc
     1621 acactcctgc tcggtctgcc gaccagctcc tcccacgcgt tgatcggtgg acttgtcggc
     1681 gctgcggtga ctcacggagg gtgggagacc gtgaaattga ccggccttcg ttcggtcttg
     1741 gaagcgatgg tgttgtcgcc gttctgcggg ttcgcgatgg gacttatttt aatggtgttg
     1801 atcagctgga cctgcttccg tatcgcgcgg agcgtggcga cgcgattctt caaacggcta
     1861 cagctcttgt cggcctgctt catggcgttc agccacgggg cgaatgatgc gcagaaggcc
     1921 atgggtatta ttacactggc tctcctctcg gcaggacaga ttcaaacggc ggaggtgccg
     1981 ggttgggtga tcggagcctg tgccatcgcg atgggcctcg ggacggcggt gggaggctgg
     2041 cagattgtcc ggaccttggg gatgcgcatc gtcaagctcg aaccagttca tgggttcgcc
     2101 gctgaaaccg gtgctgcggc ggtgctgatg gtcacggcgc acatcggttt gccggtcagc
     2161 acgacgcaca cgattacctc ttcagtgatg ggtgttggcg cgatcaagcg gctgtctgcc
     2221 gtacgctggg gggtgacgaa gcggattctc tacgcctggt tgtttacatt gcccggtgcg
     2281 gccctgttgt cgtgcgtgat ttacctgctc ggaaccaaac tgagctgagt gtccgacgaa
     2341 accacgtggt aggcgggcaa tcgcaccacg aaacggctcc cggtcgggat gttcgcctct
     2401 acccacacct gcccgccgtg tccttctacg atatgtttca caattgcgag tccgaggccc
     2461 gtgccgccca gttcgcggga acgtgcctta tcgacacggt agaatcgctc gaagacgcgg
     2521 gggcgatccc gctcggggat gcccatgccg gtgtcggtga ccgtcaagtc gatctgatcg
     2581 ctggtggagc tggcggaaga ggggcctgac gtcgcacggt gagccgcgac cgtgacggtt
     2641 ccatgttccg gtgtgtactt gatcgcattg tcgatcaggt tcatgagcac ctgcacgaga
     2701 cgctcttcgt cgccggcgac cagcgggaga tccggagcga cccacgacgt caagcgatgc
     2761 ccttttttat cggcaagggg tttgagcatg gccaaggtcc gctcgatgac cgtgctgagg
     2821 tgcaacgggt cccgcttgaa caggacttga ccggactcga tcttcgagag ctggagcagg
     2881 tcgtcgagga ttaagttcag tcggtcgctc tgtttgagaa tgatgtcgag gaagcgcgcg
     2941 ctcgtttcag ggtcattctt tcctccgtcg atgagcgcct cgacgtaccc tttgatcgag
     3001 gtcagcggag tccgcaattc atgggacacg ttcgccacga aatctttgcg gatgttctcg
     3061 agcctccgga gctcggtaat atcgtggagc accagcactg cacaggcctc gttatcctgt
     3121 tcaccgccgg cgatggaggc ttcgacatgg agacaccgcc cgctggggga gaggatgatc
     3181 tcggattctt gtccattgcg tgacgccaac acgttcgaaa ccaactgatt caagtcttga
     3241 tgctggaaca cgtcggcgca gcggcgccca cgcgcttccg atcggctgat atcgaacatg
     3301 cgctcgaggg ccggattgac ctgaaggacc cgaccgcgat agtcgaggac catcacccct
     3361 tcgaccatcg aggtgagcat cgccagcagc tgcgatcggt cttccgacaa ctcctcgatt
     3421 ttcacgcgga gctcggcggt catgtgattg agggcgtcgc ctaagagccc cacttcgtcc
     3481 tgtgccgagg tttggatatg aacgccatgg tcaccggcgg ctagctgact ggccgccttg
     3541 gccatatccg ataacggttt tgtcagcccg cgtgcgagcc acacgctcag tgcgacggcg
     3601 atgaggaacg cgcttccgaa cgcgagggtc agattgtgct gcaacttgga taactcctgt
     3661 tctaacatcg tcatcggaag cccgagcctc aggaagatcg aataggtcga atcgccatcc
     3721 cgtttcaatc cgactgctcg atacagcgtt cgctcgccgg ttgtgtgact ggtgcggaga
     3781 tccgttccga atccggaggc gatcgcgctt tgaatttccg gtctggtgcg atgattttca
     3841 acggccgcga gttcgccatc ggggacggca ctgtcggcca ataccgatcc atccattgtc
     3901 acgagcgtga tgcgcacgag cgccaacgag ctcaaatcac ggagggtggc ttggaggcgc
     3961 gttgtgggcg gcagagggtt gggacccagg agaagaggtc gtaattgatg ggccacgagc
     4021 tgggttcgga cttccagtgt ttgactcgac tgagcaatct cctgttgctt gatcgactga
     4081 gcggcgagga ggccggcgat gataagcccg caggcgagca ccaccagcgt ggcgagcgtc
     4141 accttccatc ggatgggaag cgtcatgctg tgcggtccgc ttctttcaac ttatacccga
     4201 tcgatttgac cgaggtgatg gcttcgttca ggaacggaat tttttgtttc aggcggcgga
     4261 tatggacgtc gacggtccga gtcgtaccgt aataatcata tccccacacg gtattgagga
     4321 gcacctcgcg cgtgaggacg cggccaggat ttcgcagcag gtgctcgaga agcccgaatt
     4381 ctttcgcagt caaggggatg tctgtgttgt tcagggtgac ctcgtgacgg gccaagtcca
     4441 tgactaggcg tccgtattga tatcgagtga gacgttcgtc gggtttccga tcaagccgcc
     4501 ggaagagtgc tttgacgcgc gccatcagcg ctttgggact gaagggcttg gtgacatagt
     4561 catcagcgcc caactcaaga ccgatcacgg tatccgactc ctcggctttg gccgtcaaca
     4621 tgataatggg caacatcgca gtttctggag ccgaacgaag ccgcttgcag acctcgagtc
     4681 catcgagttc ggggagcatg agatcgagga tgatcaggtc aggcttctcc tgcttgacct
     4741 gcttcagacc ttccgacccg gtcgctgccg tgacggttcg gaatccctct ttctcgagat
     4801 acaatttgac caactggagg atatcctgtt cgtcctcgac gatgagaatc ttcttcgaca
     4861 cggcaggcga catgtggata gctctccagc ctacgggggc gtgaaaaccc tgtcaagaga
     4921 cgggatttgc cgggggacgg gcaaatctgt tgacggaacc tgttgatctg acgtaaggtg
     4981 aacgaaatca aggtgctggt tcggcgtaag gccggcatgc agggtggtgc catgagggag
     5041 agcgaatcac gatgaagatc tatcttgcca acccccgggg attctgcgcc ggagtcgatc
     5101 gggcgatcga tatcgtcgat ctgtcgctga agaaatatgg tgcgccgatt tttgtgcggc
     5161 acgaaatcgt gcatagccgg catgtcgtga actcactccg tcagaaggg
//
LOCUS       DATDNW010000140         7119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_430788, whole genome shotgun sequence.
ACCESSION   DATDNW010000140 DATDNW010000000
VERSION     DATDNW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430788"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1081
                     /locus_tag="VI359_03525"
     CDS             299..1081
                     /locus_tag="VI359_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973357.1"
                     /translation="MHDLTKGIGVLLGLIMLMGCAATFPPNRLDSYLAPETKTATQDL
                     TKIDQRPLKAGLVLVSDTSAPGAAPNLPDEAFIRLGEELKQQVSRVLPVTVTEVLSAE
                     GIRPQPSGDWAQFTELGRTHKLDYLVVVVLSSTEQEYPMTLFLGWTTHAQPGYRRDNY
                     SLLEFALLDLKNNQTLMRAEARGWATLDRPSAPGIDQWYPVIYLRPQDPERRIWPPTY
                     EGAPNTLRVVSFNDAAKRLTFRLQNSWLGQLEADAAMRRASS"
     gene            1246..2751
                     /locus_tag="VI359_03530"
     CDS             1246..2751
                     /locus_tag="VI359_03530"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY6973358.1"
                     /translation="MVMWSREKNALVALVCLCVVAVACSTLPTGSMASAAGVPPSMAQ
                     GFSEIVKKVTPAVVNIAVTGGGEAGGRGRRPLPPGPFGGPPGEEPPGGELPTPPPMPP
                     PGPHGRPDQSAGSGVIVDSSGYIVTNNHVVEGATQITVTLSDRREFSAKIIGTDPKTD
                     LAVIKIEVKDLPALKWAEYEKLQVGDLVLAIGSPFGLSSTVTLGIISALGRGNVGIAD
                     YEDFIQTDAAINPGNSGGALINMNGDLIGINTAIFSRTGGSEGIGFAIPSSIALDIVE
                     SLQKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSYEAGIKRGDV
                     VIAFNGKDVQSVSQLRNLVARTMVGKDAQVKILRDGREQTLNVKVAERPSDEMLAKKE
                     PAPKEPTDTIKPPDNVLAALRIQALDPAMMSQMNIPAKTTGVVIASVEAGSSAEAAGL
                     QRGDVIQEVNHEVVKSLDDYQKAANKIKKDELAVLLLSRQGNNLFVAVNPK"
     gene            2829..3773
                     /gene="tatC"
                     /locus_tag="VI359_03535"
     CDS             2829..3773
                     /gene="tatC"
                     /locus_tag="VI359_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY6973359.1"
                     /translation="MANDSLNSINRWLQDTIFTPLEDKKMPVMEHLVEFQVRLTRAVI
                     VTAVVFVGTFFYADTLVKWLRVPLQNMFVPGSLSWVPTDLPAIPFVFLAPAEALWQNV
                     KVAGLFALVLAMPYILWEIWQFVVPGLHAQERRFVGPFVLLSTLAFYAGVGFSFFFVL
                     PFALNFLIAYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLAITLMAKLGWVDAP
                     FLKRYRKWALLGAFIVAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGFFHKKKPKTE
                     ETAAPAVAAGTSGKSASPRVPSAVEGEYVGVPSGIPRR"
     gene            3820..4773
                     /locus_tag="VI359_03540"
     CDS             3820..4773
                     /locus_tag="VI359_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY6973360.1"
                     /translation="MPRELNVIQPQGSGSGDACTYMWACAICDENESCQKDKEGHSRW
                     LVAKRMERIEYKVLIMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKMV
                     GAEGQKLKISTSGGIIPFQAYNLKIASMSFLLQNPDDPIIWRDAYKYEFINQLLGGVE
                     WQDLNFLFVDLPPGTGNESVTTIDLLGNVSGAVIITTPQEVALLDSRKSVTFCKDSEV
                     PIIGIVENMSGLECPHCHNHIEVFRKGGGEASAYDMGVPFLGRIPLDPDVVTQSDAGE
                     PFAMFNSDTSTAEAYHEIANKVEAFCKKSGSLVKVAPRHAH"
     gene            4786..6084
                     /gene="glp"
                     /locus_tag="VI359_03545"
     CDS             4786..6084
                     /gene="glp"
                     /locus_tag="VI359_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY6973361.1"
                     /translation="MKAKDATQGLTPLTDAQKVVLDATPVLGLEKISILDALGRVLGE
                     DIIAERDNPPWDNSAMDGFAVRWEDIKQEHAIQKPVALSIIEDVPAGKMPSKTVGSGQ
                     AIRIMTGAPIPKGADTVLKVEDTEHTPEAVRVFKPEQKGSNIRPQGEDVRKGECIIAK
                     GTQIRPGEAGMLAILAKSFVFVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQDAGGIPFLLGIARDNPAALKEKISHGLTADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKMSGHRSYG
                     RPLVQAVFQEKFSKRTDRRHFLRGVLTREDGVFKVHTTGDQGSGILTSMVKANCLIDV
                     AVEVERLNPGDLVTVQLLSGEAWSNHADQNQSGSHRLSCC"
     gene            6106..6630
                     /gene="mobB"
                     /locus_tag="VI359_03550"
     CDS             6106..6630
                     /gene="mobB"
                     /locus_tag="VI359_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY6973362.1"
                     /translation="MAVPIVCFVGRSNSGKTTLIERVIPELVRAGYKLATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVVISKGSMAMFADVSDQMHVTDVRDRFLDESYDLIIAE
                     GWKSEGYPKIVIVREQLGEVPVSQEGLLAVVSDKPVDLQVPVFDINDVVGVAALIMTK
                     FPKRHPRAAHELEA"
     gene            6614..>7119
                     /locus_tag="VI359_03555"
     CDS             6614..>7119
                     /locus_tag="VI359_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY6973363.1"
                     /translation="MSWKPKVTIILGLIAGAMALSGVVIPLTSHPKFCASCHNIAPSY
                     ESWTKSSHKDVTCVACHVRPGLEGWVHDKAWNGMKDVAIYIFGTPTDPHNLKARVDSN
                     VCLSCHRNILRMSEVAPRDLPPPVKEVGLVMSHRKHIEAFSARGQGEGCTTCHSGIVH
                     ERPIKGYP"
BASE COUNT         1558 a         1836 c         2173 g         1552 t
ORIGIN      
        1 aatcgcgtga aggtaaggaa gaatccgggc agcttcgtta tgagccggaa tgacaacaga
       61 aatatcgtga agggcgaggg gatctcgtga cggcacgggt gattgttccc tgtagtcggt
      121 caacagcctc gaaaccacac gagcagcggg ggtgagttac ggtagctgag cgtcatgcag
      181 aattgcaaga gagaggaagg ttcaggggta ttgacagatc tggtcatggg gggcgaacgt
      241 atacatgaga gacagagtgc tcacgatagg ccccacgaat tcagccggga ggtgagatat
      301 gcacgatttg acgaagggaa tcggtgtcct gctcggtcta atcatgctta tgggttgtgc
      361 ggcgaccttt ccgccgaatc gtctcgacag ctatcttgcc ccagaaacga agaccgccac
      421 ccaagatctc acgaaaatag atcaacggcc gctgaaagca ggcttggtgt tggtatcgga
      481 tacctcggct ccaggcgcag caccgaatct gccggacgaa gcgttcattc gcttgggcga
      541 ggagttaaag cagcaagtca gccgggtgtt gccagtaacc gttaccgaag tgctatcggc
      601 tgaagggatc cggccgcagc caagcggaga ctgggctcag tttaccgagc tcggcaggac
      661 ccacaagctc gattacttgg ttgttgtcgt tttatccagc acggagcagg aatatccgat
      721 gacactgttc ctaggctgga cgactcatgc gcagccgggg tatcgtcgcg acaactactc
      781 tctgctggaa ttcgccctgc tggatctgaa gaataatcag acgttgatgc gggcggaggc
      841 tcggggctgg gcgacgttgg atcgcccttc agctcctggc attgaccaat ggtatccagt
      901 aatttatctc cgtccgcaag atcccgaacg gcggatttgg cctcctacct atgagggtgc
      961 accaaatacc ttgcgggtgg tctcgtttaa cgatgcggcc aagcgcctca cttttagatt
     1021 acagaactcc tggcttggtc agttagaggc cgacgcagcg atgcgcagag ccagttcata
     1081 gacctattgc ggacacagct gccttgcggc cttacctcgt ccccttcggg cctgctttgt
     1141 ggtccggagg ggacttttag tctttgaagg cttctgaata gatagtgtaa aatacaccgt
     1201 tcggcttctg gttcgtgcca cctatttttc tggaggaacg ctgttatggt catgtggagt
     1261 cgggaaaaga atgcgctcgt tgccctggtc tgtttgtgtg tggtggccgt tgcatgcagt
     1321 accttgccaa ctggctcgat ggcgtcggct gcgggggtac cgccatccat ggcgcagggc
     1381 ttttccgaga tcgtgaaaaa agtgacgccc gcggtggtca acatcgccgt cacgggtggg
     1441 ggagaagcag gagggcgagg acgtcgtccc ttacctcccg ggccgtttgg cggacctcct
     1501 ggggaagaac ccccgggtgg tgaactaccg acaccgccgc ccatgccgcc gccgggtcct
     1561 cacggccgtc ctgatcaaag tgcgggctca ggcgtgatcg tcgactccag tggctacatc
     1621 gtcaccaaca accacgttgt tgaaggagcc acgcagatta ccgttacatt gagtgatcga
     1681 cgcgagtttt ccgctaagat catcgggacc gatccgaaga cggacttggc cgtcattaag
     1741 attgaagtca aagaccttcc cgctctgaag tgggcggagt atgaaaagct ccaagtaggc
     1801 gacctggttc tcgccatcgg cagcccgttt gggcttagtt ccacggtgac gttggggatt
     1861 atcagcgcgc tgggtcgtgg aaacgttggt atcgctgatt atgaggattt cattcagacc
     1921 gatgcagcga tcaatccggg taattccggt ggggcgctga ttaacatgaa cggggacctg
     1981 atcggcatca atacggcgat cttttctcgg accggcggat cggagggaat cggctttgcc
     2041 attcccagca gcatcgcgct ggatatcgtt gagagtctcc agaaaaccgg caaagtcgtg
     2101 cgagggtgga tgggggtggc gattcaagag atcacgcctg cgcttgcgaa atcgtttaag
     2161 cttcccgagc aacgaaaagg ggtgctcatc agcgacgtga acgagaacgg tccctcttat
     2221 gaggctggga tcaagcgggg cgatgtggtg atcgcgttta atggaaaaga cgtccagagc
     2281 gtcagccagt tgcggaatct cgtcgcacgg acgatggtag gcaaagatgc ccaggtcaaa
     2341 attctccggg acgggaggga acagactctg aacgtgaagg tcgcggaacg accgtccgac
     2401 gagatgctgg ccaagaaaga gccggctccg aaggaaccga ccgacacgat caaaccgccc
     2461 gataatgtgc tggccgcgct tcgcatccaa gccctcgacc cggcgatgat gagtcagatg
     2521 aatatccctg cgaagacgac cggggtcgtg atcgcgtcgg tggaagcagg cagctcggca
     2581 gaggcggctg gcttacagcg gggcgacgtg attcaagagg tgaatcacga agtcgtgaaa
     2641 agtctcgacg actatcagaa ggcggcgaat aagattaaga aagatgaatt ggcggtcctg
     2701 ctcctcagtc ggcaaggcaa taacttgttc gtggcggtga atccgaaata gacacgtcac
     2761 ggttgaggtg caaggctgag gactgtgttg ttcttaaccg ccacctcgac cgcgtgtgca
     2821 tctcccttat ggcgaacgac agcttgaaca gcatcaaccg gtggctgcaa gacacgatct
     2881 tcacgccgct ggaggacaag aaaatgccgg tcatggagca cctggtggag ttccaggtgc
     2941 gcctgacccg ggctgtcatt gtcaccgctg tcgtgttcgt cggcaccttc ttctatgccg
     3001 atacccttgt gaaatggctt cgtgtgccgt tgcagaacat gttcgtgcca ggttcgctgt
     3061 cctgggtacc gactgatctt cctgccatcc ctttcgtctt tctggcaccg gctgaagccc
     3121 tctggcagaa cgtcaaggtc gctggactct ttgcccttgt gctggcgatg ccgtacatct
     3181 tgtgggaaat ctggcaattc gtcgtgcccg gcctccatgc gcaagagcgg cgtttcgtgg
     3241 gtcccttcgt gctcttgagc acgttggcat tttacgccgg cgttgggttt tcgtttttct
     3301 ttgtcctgcc gtttgctctg aatttcctga tcgcctatgg ggtgaatgcc gggtttattc
     3361 cgcagctgtc gatcgcccag tacgtcgggt tcgctctgtg gttcttgctt gtgttcggac
     3421 tgatcttcga agtgcccttg gcgattacgc tgatggccaa actgggatgg gtcgatgcac
     3481 cgtttttgaa gcgctatcgg aaatgggcgc tgttaggggc tttcattgtt gccgcaattc
     3541 tgaccccgac accggacccg ttcaaccagt gtttgatggc cttgccgatg tatatctttt
     3601 atgaagtcgg gatcgtcagt gcgggcttct ttcataagaa aaagccgaag acagaagaga
     3661 ccgccgctcc ggccgttgct gcagggacca gcggtaaatc agcctcccca cgagttccga
     3721 gtgcggtgga aggggaatac gtcggcgtac cgtcaggaat ccctcggcga tgagcatgat
     3781 ggactaatag accagctagg taaggagtca tgatcaccca tgccacgtga attaaatgtc
     3841 attcagcctc aaggatctgg aagcggcgat gcttgcacat acatgtgggc ctgtgccatt
     3901 tgcgatgaga atgagtcgtg tcagaaggac aaggaagggc atagccgctg gcttgtcgcc
     3961 aagcgcatgg agcgcatcga atacaaagta cttatcatga gcaacaaggg tggcgtgggg
     4021 aagagtacct gcaccaccaa cttggctgtg agcctggccc tcaaaggctg gcatgtgggt
     4081 atctgcgaca tggacatcca tggaccgaac atcccgaaga tggtcggggc cgaaggccag
     4141 aagttgaaga tcagcacatc gggcgggatc attccgttcc aggcctacaa tctcaagatc
     4201 gcatcgatgt ccttcttgtt gcaaaatcca gacgatccga tcatttggcg cgatgcctac
     4261 aagtacgaat tcatcaatca gctcttgggc ggcgtcgaat ggcaagatct gaatttcctg
     4321 tttgtcgact taccccctgg aaccggcaac gaatcggtga cgaccatcga tctgctcgga
     4381 aacgtgagcg gcgcggtcat tatcactacc cctcaagaag tggcgctcct cgattcccgc
     4441 aagtccgtca cgttttgcaa ggacagtgaa gtgccgatca tcggcatcgt ggagaacatg
     4501 agcggattgg agtgcccgca ctgccacaat catatcgaag tcttccgcaa gggcggcggt
     4561 gaagcctctg catacgatat gggtgttccg tttcttggcc gaattccgct cgatcccgat
     4621 gtcgtgaccc agtctgatgc cggtgagcct tttgcgatgt tcaattctga cacgtcgacg
     4681 gccgaggcct atcacgaaat tgccaacaag gtcgaagcgt tctgcaagaa gagcgggtcg
     4741 ctcgtgaaag tggctccgcg gcatgcgcat tgacgagggg gcaaagtgaa agcaaaagac
     4801 gcgacacagg gcctgacgcc gttgactgac gcgcagaagg tcgtactgga tgccacaccg
     4861 gttctgggct tggagaagat ttccatactc gatgcactcg gtcgagtgtt gggggaagac
     4921 atcatagcgg agcgcgacaa tccgccgtgg gacaactcgg cgatggatgg atttgccgtg
     4981 cggtgggagg atatcaaaca ggaacacgcg attcagaaac ccgtggcgct gtcgatcatc
     5041 gaggatgttc ccgcaggaaa gatgccgtcg aagacggtcg ggtctggtca ggcaattcgg
     5101 atcatgacgg gagccccgat tccgaaaggt gcagacaccg tgttgaaagt cgaggatacc
     5161 gagcacacac cggaggctgt gcgagtgttc aagccggaac agaaaggttc gaacattcgt
     5221 ccgcagggcg aagacgtcag gaagggcgag tgtatcatcg caaagggcac tcagattcga
     5281 ccgggagaag ccgggatgct ggcgatcttg gcgaagtcct tcgtctttgt ctatcaacgt
     5341 ccgcgcgtgg cgattctgtc caccggagat gaacttgcgg acttggatga gcggttcagc
     5401 gaagagaaga tcatcaattc aaacagctat gggattgcgg ccgccgtgca ggacgccggc
     5461 ggaatcccgt ttctgctcgg tatcgcgcgg gacaatcctg ccgcgctcaa agagaagatt
     5521 tcccacggct tgacggcgga tatcttggtc ttgtccgggg gagtctccat gggcgactac
     5581 gactttacca aggccgtatt tcgcgatctg ggagccgaga tgaatttttg gaaactcgcc
     5641 atccgaccgg ggcagccgct ggccttcggg aagattcagg gcaaactcgc gttcggtctg
     5701 ccggggaatc ccgtctcatc gatggtcacg ttcgaacagc tggttcgccc agcgatgctg
     5761 aaaatgagcg gccaccggag ttacggccgt cctctcgttc aggcggtctt ccaagaaaag
     5821 ttttccaagc gaacggatcg ccgtcatttt ttacggggag tcttgacgcg agaagatggc
     5881 gtctttaagg tccacacgac cggtgatcaa gggtcaggaa ttctcacctc gatggtcaag
     5941 gccaattgtc tgatcgatgt ggcggtggaa gtcgaacgac taaatcccgg cgatctcgtg
     6001 acggttcagc tcttaagcgg cgaagcttgg tcaaaccacg cagaccagaa ccagagcggg
     6061 tcccaccgcc tgtcctgttg ttgagggggg aagggacgtc ggctcatggc tgttcctatt
     6121 gtctgtttcg tgggtcgctc aaacagcggc aagaccaccc tcatcgagcg cgtgatcccc
     6181 gagctggtcc gggcgggtta caaactcgcg acggtcaaac atgccggtca tgggtttgat
     6241 ctcgacaccg aaggaaagga cagttggcga cacaagcagg ccggtgccag cagtgtggtg
     6301 gtgatctcca aaggcagcat ggccatgttt gccgatgtct ccgatcagat gcacgtcacg
     6361 gacgtccggg ataggttttt ggacgagtcc tacgatctga tcatcgccga gggctggaag
     6421 agcgaaggct atcctaaaat cgtcattgtc cgcgaacaac tgggagaagt gccggtctca
     6481 caagaaggac tgttagcagt ggtctccgat aaacccgtcg atcttcaagt tccagtattt
     6541 gacatcaacg atgtcgtcgg cgtggccgcg ttgatcatga cgaagttccc caagcggcat
     6601 ccgcgcgctg cgcatgagct ggaagcctaa ggttaccatt atattgggcc tgattgccgg
     6661 tgcgatggca ctgagtggcg tcgtcattcc tctcaccagt catccgaagt tttgtgcgag
     6721 ctgccataac attgctccgt cctacgagag ttggacgaag tcttcccata aggacgtgac
     6781 ctgtgttgcg tgtcacgtgc gacccggtct ggaagggtgg gtgcatgaca aggcatggaa
     6841 tggcatgaag gacgttgcca tttacatttt cgggacgccg actgatcccc ataatctcaa
     6901 ggctcgggtc gactccaatg tgtgcctcag ctgccatcga aacattcttc gcatgtcaga
     6961 agtggcgccg cgcgatctcc cgccgccggt gaaggaagtg gggttggtga tgagccatcg
     7021 aaaacacatc gaggcgttct cggcaagagg acaaggggaa gggtgtacga cctgtcattc
     7081 ggggatcgtg catgagcgcc cgatcaaggg ctatccgat
//
LOCUS       DATDNW010000141         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_699196, whole genome shotgun sequence.
ACCESSION   DATDNW010000141 DATDNW010000000
VERSION     DATDNW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699196"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VI359_03560"
     CDS             <1..1134
                     /locus_tag="VI359_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY6973364.1"
                     /translation="DGWLFYDFRGSDPLAYRILLLDPTLHVTRRWYYWVPAQGTPVKI
                     VHRIEPHILASLPGRAECYVSWEQHHRLLAEVLTGTRRVAMQYSPLNAVPYVSRVDAG
                     TIELVRGSGVDVVSSADLVQTFEARWTDCQLESHRVAAAALRRIVDEAFGHVQARISH
                     GQVLSEYGLQQFILSRIHDVSMVTSSPPIAAVNAHSADPHYGPAPTGSAELKRDDLVL
                     IDLWAKQAEPGSVYADITWTGYIGGHAPAKQSRIFNIVRQGRDAALAYVQASVAGGQF
                     PYGWEVDQACRRVIEAAGYGDHFVHRTGHSIGEEVHGNGANIDGLETQDTRRLIPRTC
                     FSIEPGIYLAGDFGIRSELDVYISEHEASVFGLPLQQEIIPIL"
     gene            1248..1373
                     /locus_tag="VI359_03565"
                     /pseudo
     CDS             1248..1373
                     /locus_tag="VI359_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            2147..2848
                     /locus_tag="VI359_03570"
     CDS             2147..2848
                     /locus_tag="VI359_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6973365.1"
                     /translation="MPDRAAKLLIELLQDPHPDIRRVAAESLGKIGDPRTGSALVPLL
                     TDPDAGVREAAVRAIGRLGPSAGEEVAQVVVALLDDPNESVRRAVTIAIGEIEPPTRV
                     FVAVPKLLRARDVSTRRSAAQALSMIEARAWLPDLVKALQDPDTEVRQHVVAALAELG
                     DPIIAPLLRDRLARDPSVAVRTEAAYRLGKIGDPETKEVLAAVAGRDPDRGVRQWARM
                     GLDAARSSTESGSMR"
     gene            complement(2814..>3273)
                     /locus_tag="VI359_03575"
     CDS             complement(2814..>3273)
                     /locus_tag="VI359_03575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973366.1"
                     /translation="NQEAVLLRGAKGQLIGTMPWSGVIDRILANDDDARFAHSRAHPR
                     APLAVKVRYMTPDGKQFDSLTGGIGGGGLFIESSTPLAPGTELSVEFALPDRPWERLK
                     AKAKVAWTRNKPERYLLFPGMGVQFTDIDPGCQRQLVELVTALNRTRLTT"
BASE COUNT          668 a          915 c          972 g          718 t
ORIGIN      
        1 gatggctggc tcttttacga ttttcgggga agcgacccac tggcctatcg cattctatta
       61 ctcgatccaa cgttgcacgt cacgaggcgt tggtactatt gggttccagc tcaaggcacg
      121 ccggtgaaaa tcgtgcatcg aattgagccc cacatccttg cgtcactccc tgggcgagct
      181 gagtgctatg tctcgtggga gcaacatcat cggttgctgg ctgaagttct cacgggaacc
      241 cggcgagtgg cgatgcaata ctctccattg aacgctgtgc cctacgtgtc ccgggtggat
      301 gcagggacga tcgagttggt taggggatcc ggtgtcgacg tggttagctc ggctgatctc
      361 gtccagacgt tcgaggcacg gtggacggac tgtcagctcg agtcgcatcg tgtggccgcc
      421 gcagccctgc ggcgcattgt cgacgaagcc tttggccatg tgcaggcgcg aatcagtcac
      481 gggcaggttc ttagcgaata tggattgcaa caattcatcc tttcgcgcat tcacgacgtg
      541 agcatggtta cgtcgagccc tccaatcgca gccgtcaatg cccacagtgc cgacccccac
      601 tatggccctg caccgacagg gtccgctgag ttgaaacgag atgatctcgt gttaatcgat
      661 ttatgggcca agcaggcgga gcccggttct gtctatgccg atatcacctg gacgggctat
      721 atcggcgggc acgctccggc gaaacagagc cgcattttca atatcgtgcg gcagggccgc
      781 gatgccgcgc tcgcctatgt acaagcgagt gtggccggtg gacagtttcc ttacggatgg
      841 gaagttgacc aggcgtgtcg acgtgtgatc gaggctgctg gatacggcga tcactttgtg
      901 caccggaccg gtcattcgat cggagaagaa gttcacggca acggggccaa tatcgacggc
      961 ctcgaaacgc aagacactcg ccgtctcatt cctcgaacct gtttctcgat cgaaccaggt
     1021 atttatttgg cgggggactt cggcattcgt agtgaactcg atgtctatat aagtgaacat
     1081 gaagcctctg tgttcgggct gcctctccaa caagaaatca ttccgatcct ctaaaagtcc
     1141 ggctggtcat tccttgacac cgtgttgtat cggcctataa agttgaagca gttatcgtga
     1201 ggctgggtaa acagcctgtt cacctttctc atggtgggga acataccatg ttcggtacaa
     1261 tggggatttc cgaactcatc atcatccttg cgatcgtcct gatcatcttt ggcgccggtc
     1321 ggttaccgca aatcggagag ggtttcggaa aggctctcaa gggattcaag aaggaagtcc
     1381 acgatattcc accgccagaa ccggctcagg cagaagctgc tccgaccact caggcgcaac
     1441 cagttgcgca agcggtagcg agtcaggctg cccatcaggc tccaactcct caaaccctca
     1501 ggccgacggc tccctatacc cctggaccgg aactcacccc agggaccacc gctgcattga
     1561 tgtataacgt gggagcccaa gcagcacagc cggctcggcc ccagtcgacg acagcgacat
     1621 cagccggaca aggtcagcag cctcccacga tggaagaacg gaccgctagc ccgactccga
     1681 tgatgcgtgc gcagtatcct ccccttcccc agagtgctca gcagaagccc tcagcgaagc
     1741 gtccttctgc agttgtgaac aaagatgcgg tggctcgtgt tcaagcacag caggcggcga
     1801 tgaaggctaa ggccacccag caggcgggag tatcccctgg tgatatgcaa agtttgggcg
     1861 aagggctcgg tgatgccctg cggacgtttc gtcaagcagt ggccgacgtt cgtcacactg
     1921 ttgatcctga gatgcgcacg attcaggccg aaatggatgc ggcgcagaaa gagttggaac
     1981 aatccatcga atcggccaag gaacttccga cgcttcacga agagcctccc aagcaggcgt
     2041 gatatcccac cgacttcttt ggttccattc ccctttttac ggcctcatcg tatggtgcgt
     2101 tgtagggttg gtggtcggtg gctgttatgg caatgaacct ccgacaatgc ccgaccgcgc
     2161 agccaagctg ttaatcgagt tgcttcaaga tccccatccg gacattcgcc gtgttgcggc
     2221 tgagtccctg gggaaaatcg gtgacccaag gaccgggagt gcgctggttc ccttgttgac
     2281 tgatccagac gcaggcgttc gtgaggccgc ggtgagggcg attggccgcc tcggtccctc
     2341 cgctggcgag gaagtggcac aggttgtcgt ggcattactt gatgatccaa atgaatccgt
     2401 caggcgagcg gtgacaattg cgataggcga gatcgagcca ccaactcgtg tgtttgtcgc
     2461 ggttccgaag ttgttgagag cccgagacgt atcgacgcga cggagcgccg cccaggccct
     2521 ctcgatgata gaggccagag cgtggcttcc cgatctcgtc aaggcgttgc aagatcccga
     2581 tacagaagtg cgtcaacatg ttgttgcggc gttggccgaa ctcggcgatc cgatcattgc
     2641 gccgttactt cgtgatcggt tagcacgtga cccgtctgtg gcggtgcgaa cggaagcggc
     2701 ctatcgcttg ggaaagattg gagaccccga aacgaaagaa gttttggccg ctgtggctgg
     2761 tcgggaccct gatcgtgggg ttcgtcagtg ggcgcggatg ggtctggatg ctgctaggtc
     2821 gtcaaccgag tccggttcaa tgcggtgacg agctcgacta attgccgctg gcaaccgggg
     2881 tcaatatctg tgaattggac tcccattccc gggaacagga gatagcgttc tggtttgttt
     2941 ctggtccacg ccacttttgc tttggccttg agtctttccc atgggcggtc cgggagagca
     3001 aattccactg acaactctgt gcctggagca aggggggtgc tgctctcgat gaataagccc
     3061 cctccgccga tcccgccagt cagactatcg aactgtttgc cgtcaggggt catataccga
     3121 accttcacgg ccaggggggc tcgtgggtgg gctcgagaat gggcaaatcg cgcgtcgtca
     3181 tcattcgcaa ggatacggtc gattacccca ctccacggca tggttccaat cagctgcccc
     3241 ttggctcccc gaagtaagac ggcttcttgg ttc
//
LOCUS       DATDNW010000142         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_86394,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000142 DATDNW010000000
VERSION     DATDNW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86394"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VI359_03580"
     CDS             <1..304
                     /locus_tag="VI359_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6973367.1"
                     /translation="VEQKKIDLMNTISLEVTDAYLTLQAARQQVIVEEKEVESARSAL
                     TLAKERYRLGLASIVDVTTATTELLMAEVRLSEARYALQAGSAAVAYATGQAYQSF"
     gene            365..2293
                     /locus_tag="VI359_03585"
     CDS             365..2293
                     /locus_tag="VI359_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973368.1"
                     /translation="MRWPLQFLNDLPIARKLFLASVIPVLTVVVLSILTYRSVVTFSE
                     DEGELNNIYVAQRLAAEYLRLVVDLETGFRGFIMTKQEKYLYPYRTAQDHIRNVGSSL
                     EEKVHSQQEQRDLVAKVQELVKQFAEEKEVLIEDVKAGNQEKARIYIEESRGRQIMRK
                     IRESMARLDQLEHDVLNARLAKLYQDRNAMLTVILGGGLFALVLMVFALHLIGRSITG
                     PLAALAKAVRTSPSGLVPTVPVMDRKDEIGNLTNMMHAMSAQIRDHLAAVEKSETELR
                     ALNQDLSASESKYRSLVDHAPFGIFTTKGMAMVFSNRYNRVLAGLNPDEEGDPEAIRS
                     AIHPEDRDRVLSEFEKAVKEGRPYETVFRFLHKDGMVRKVLSRRIPIKDTGGQVVMYQ
                     GFNIDITALDQMQTRLSRSERLATLGQVAAGIAHEIRNPLVGIGSTTSLLVDDTDVKD
                     PRRSDLEVILRETRRLDRIVNQIIEYARPRELAPVLFSFADLVQEVLKLLDAPLSTKA
                     LKVTCAIPPSLPQVHADRDQMKQVLLNVFQNAIEASGDGSGLSVAAGPFARNLDPGIV
                     ITVTDQGPGISSEDLPHVFEPFFTRGKHRGTGLGLAICRNIVDAHGGDIQLTSGRGKG
                     TTARLWLPLRQQPRLIEV"
BASE COUNT          543 a          660 c          715 g          514 t
ORIGIN      
        1 ggttgagcaa aagaagatag atctgatgaa cacaatttcg ctggaagtga cggacgccta
       61 cctgacgctg caagcagccc ggcaacaggt cattgtcgag gaaaaagagg tggagtccgc
      121 acgcagtgct ctgaccttgg ccaaggagcg ctatcggctg ggactagcgt caatcgtgga
      181 tgtcaccaca gcgacgacgg agctgctcat ggccgaggtc cgtctctcgg aggcgcggta
      241 tgccttgcag gcgggatccg cggctgttgc ctatgccacc ggccaggcct atcagagttt
      301 ttgacccgcc tccctcgatt gactatgatc atggggcttt ctaggatagt ttcatgaggg
      361 cttgatgcgc tggcctctgc aattcctcaa cgatctgccg attgcccgca agctgttcct
      421 tgcgtcggtg atccccgtcc tcacggtggt ggtgctcagc atcctgactt acagaagcgt
      481 tgtgacgttc tcagaggacg aaggagaact caataatatc tacgtcgcgc agcggctggc
      541 agcagaatac ctgcggctgg tcgtggatct cgaaacagga ttccgtggtt ttatcatgac
      601 caagcaggaa aagtatctct acccttaccg aacagcccag gaccacatcc gcaacgtcgg
      661 cagttctttg gaagagaagg tccatagtca gcaagaacaa cgggaccttg tggccaaggt
      721 tcaggaattg gtcaaacagt tcgcagagga gaaggaagta ctcattgaag acgtgaaggc
      781 tggcaaccaa gaaaaggcta ggatttacat cgaggaaagc cggggtcggc agattatgcg
      841 caagattcgc gagtcgatgg ctcgccttga ccaactggaa cacgacgtgc tcaacgcgcg
      901 cctggcgaaa ctctatcagg accgcaatgc aatgttgact gtcattcttg gcggtggttt
      961 gtttgcgctc gtgctcatgg tttttgccct ccacctcata ggtcggtcca tcactggccc
     1021 actcgcggcg cttgcgaaag cggtcaggac ttccccttcc ggattggttc ccacagtccc
     1081 ggtgatggat cgaaaggatg agatcggcaa tctcaccaac atgatgcatg ccatgagcgc
     1141 ccagatcagg gaccatcttg cggcggtcga gaagtcggaa acggagctgc gggccctcaa
     1201 tcaggacctg tcggcgtccg agtccaagta tcggagcctg gtcgatcatg ctcccttcgg
     1261 catcttcacc acaaaaggga tggccatggt gttcagcaac cggtataacc gggtcttggc
     1321 tggcctcaac cccgatgagg aaggcgatcc cgaagcgatt cgcagcgcga ttcatcctga
     1381 agacagggac cgggttcttt cggaattcga aaaagccgtc aaggaaggtc gtccatatga
     1441 gacggtgttc cgattccttc acaaggatgg gatggtcagg aaagtcttga gccggcgcat
     1501 tcccatcaag gacaccggcg gacaggtggt catgtatcaa gggttcaata tcgatattac
     1561 tgcgctggat caaatgcaaa cacgattgag tcgatcagag cggctcgcta ccctcggtca
     1621 ggtcgcggcc gggatcgccc atgaaattcg gaacccgctc gtcggaatcg gatcgaccac
     1681 gtcgctgctg gtcgacgaca ccgacgtcaa ggaccctcga cgttctgacc ttgaggtgat
     1741 tttgcgagag acgcgtcgcc tggatcggat cgtgaatcag atcatcgagt atgcgaggcc
     1801 gcgcgagttg gcgccggtgc tcttctcctt cgccgatctg gtgcaggaag tgttgaagct
     1861 gctcgacgcc cctctttcaa cgaaagcgtt gaaggttacc tgtgccatcc ctccctcttt
     1921 gcctcaggtt catgccgatc gagatcagat gaaacaggtg ttgttgaatg tatttcagaa
     1981 cgcgattgag gcttctgggg acggcagcgg gctcagcgtc gcggccggtc cattcgcgag
     2041 aaacttggac cccggcattg tgattaccgt gacggatcaa ggacctggaa tatcgtcgga
     2101 agacctcccg cacgtgttcg aacccttctt cacacggggg aagcaccgag ggaccggact
     2161 cggtctggcc atttgccgaa atatcgtcga tgcccacggc ggcgacatcc aattgacgag
     2221 cggtagaggg aaggggacga cggctcggct ctggcttcct cttcgacaac agcccagact
     2281 gatcgaggtc taatatatgc gcgcaacgat ctttgtcact gacgatgagc cggcgatccg
     2341 tgctgcaatt atcaaacgct tgtcacgacg gcaccaccgt gtcgtggggt atgactcggg
     2401 agacgccctc atgaaggcga tcgagcacga cc
//
LOCUS       DATDNW010000143         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_86643,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000143 DATDNW010000000
VERSION     DATDNW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86643"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /gene="bioB"
                     /locus_tag="VI359_03590"
     CDS             complement(<1..839)
                     /gene="bioB"
                     /locus_tag="VI359_03590"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY6973369.1"
                     /translation="MIGHQEFAEKALREEILSRSECKDVLNTSDEELLGLLQAAFRVR
                     FRYFGKTVRLQMLQNAKSGACQEDCHYCSQSAVSTASIERYNLVPQKQMIEGARQAAA
                     SKAQRYCIVISGRSPLDREIDEIAKAVQAIKKEIPIQICCSLGLMNEVQARRLKTAGV
                     DRVNHNLNTSEAFHSSICTTHTFQDRLATIKNARVAGLEICSGGIVGMGEKDDDLIDL
                     AMALRDVKPDSIPLNTLHPVAGTPLEHCDNLTPQRCLKVLCLFRFLHPRTEIRIAGGR
                     EHNL"
     gene            complement(902..>2247)
                     /locus_tag="VI359_03595"
     CDS             complement(902..>2247)
                     /locus_tag="VI359_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973370.1"
                     /translation="SYFRELNPGSITVHTGPSTHFELSAVTLVVFSMAVGAVLVALAV
                     GMRQTVHVIGNWRSTRLQRRKEKVDALHREGTHAFMSKRTSDAVGLFERALSIDPNRI
                     DSLLWLGNIYRSESNYAEAIRLHQNAQRIDDRNIEILLELSKDLEGAKRYEDALQSLQ
                     RILRIEPDNLTALTRKRDLLIRLEKWSDALEIQHRMAKANLPESERRAEAQLLTGCMY
                     EVGRQLLERGHADKARRYFRGAIKKDRTFLPAYIGLGEVLIREGKTKDAVEILKKVYA
                     KTRNVIILHRLEEHFLEQGEPGEIIRTYQEALQQDPNNAILQFYLGKLYYRLEMVDEA
                     FDILSTVEGPQDQLVDYHKIMANLFLRKQHMEQAVVELKKALQFKKRVVVPYVCTVCQ
                     QDTTEWSGRCRRCGNWNTFVALPWLDGSPAAGRESTSLPTRGIPYQGIASPFETV"
BASE COUNT          461 a          609 c          637 g          540 t
ORIGIN      
        1 agattatgct cgcgaccgcc ggcgatgcgg atctccgtgc gagggtgtaa gaaacgaaag
       61 agacacaaca ctttcagaca ccgttgcggc gtgaggttat cgcaatgttc caatggagtt
      121 cctgctaccg gatgcaacgt attgagaggg attgagtcag gtttcacgtc gcggagagcc
      181 atggccagat cgatgagatc gtcatccttc tcgcccatgc cgacgattcc tcctgagcaa
      241 atctccaatc ctgccacgcg cgcatttttg atcgtcgcga gccgatcttg aaacgtgtgt
      301 gtcgtacaga tcgaactatg aaaggcttcg ctcgtgttga gattgtggtt gacccggtcg
      361 acgcccgcgg ttttgagccg cctggcctgg acttcgttca tcaggccgag ggagcaacag
      421 atttgaatag gaatctcttt cttgattgcc tggactgcct tggcgatttc atcgatttcg
      481 cggtccaatg gacttctgcc gctgatgacg atgcagtacc gttgggcttt agaggctgcg
      541 gcctggcgcg cgccttcgat catctgcttc tgcggtacca gattgtagcg ttcgatcgat
      601 gctgttgaga cagccgattg ggagcagtag tggcaatctt cctgacaggc tccactcttg
      661 gcgttctgca acatctgcaa gcgcaccgtc ttgccgaagt agcggaagcg tacccgaaag
      721 gcagcttgca ggagcccgag aagctcctcg tcagatgtat tgagcacgtc cttacactca
      781 gaacgggaga ggatctcctc acggagggct ttctctgcga actcctgatg accgatcatc
      841 gaacgctccc aaggatggca aatgagcctg ctactgtggt gctgtcttcg gacgcgaaaa
      901 cctacacggt ttcgaacgga gaagcaatgc cttggtaggg gatgccacgg gtcggtagcg
      961 aggtgctctc tcgtcctgct gccgggcttc cgtctagcca gggcagagcg acgaaggtat
     1021 tccagttacc acaacggcga caacgtccgg accattccgt tgtgtcctgc tggcacaccg
     1081 tgcagacgta cggtacaacg acgcgcttct tgaactgcag cgctttcttg agctcgacga
     1141 ctgcctgttc catgtgctgt ttgcgcagga acaggttagc catgatcttg tggtaatcga
     1201 cgagttgatc ttgaggccct tccacggttg agagaatatc gaatgcctca tcgaccatct
     1261 ccaaccggta atacaacttg ccgagataga actggagaat ggcattattc ggatcctgct
     1321 gcagggcctc ctgataggtc cggattattt cacctggttc tccctgttcc aagaagtgct
     1381 cttccaagcg gtggagaata atgacgttcc gagtcttcgc atacaccttt tttaaaattt
     1441 ccaccgcatc cttggttttg ccttcgcgga tcagaacttc gccgagcccg atgtaagcgg
     1501 gaaggaacgt gcgatctttc ttgatcgcgc cgcggaagta gcgacgcgcc ttgtcggcat
     1561 gaccccgctc caacaactgg cgccccactt catacatgca tccggtgagc aactgcgctt
     1621 cagcgcgccg ttccgattct ggcaggttgg ccttcgccat gcgatgctgg atttcgagcg
     1681 cgtcgctcca tttttcaagc cggatgagca agtcgcgttt gcgggtcaag gccgtcaggt
     1741 tgtctggctc gatcctcaaa atcctttgca aggattggag tgcatcttcg tatcgtttgg
     1801 ccccttcgag gtctttcgac agttccaaga ggatttcaat attccggtcg tcgatgcgtt
     1861 gggcgttttg gtggagccgg atcgcctcgg catagttgga ttcagaccga tagatgtttc
     1921 caagccatag gagggaatcg atccgattgg gatcgattga cagggccctt tcaaagagac
     1981 cgaccgcatc acttgtgcgc ttggacatga aggcatgggt gccttcccgg tggagggcgt
     2041 cgactttttc cttccgccgc tggaggcggg tgctgcgcca attgccgatg acgtgcacag
     2101 tctgtcgcat gcccacagcc aaggccacga gcacggcccc gactgccatt gagaacacca
     2161 ccaacgtcac cgcgctgagt tcgaagtgag tagagggtcc ggtatgaacg gtgatagagc
     2221 cgggattcaa ctcgcgaaaa tagctat
//
LOCUS       DATDNW010000144         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_431852, whole genome shotgun sequence.
ACCESSION   DATDNW010000144 DATDNW010000000
VERSION     DATDNW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431852"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VI359_03600"
     CDS             complement(<1..891)
                     /locus_tag="VI359_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6973371.1"
                     /translation="MGLSDNFYRLKRLPPYVFAQVQSLKLEARHHGEDIIDFGMGNPD
                     QPTPNHIVEKMIEAARKGKNHRYSASRGITKLRHAIAAWYKRNYDVDLDPETEAIVTI
                     GSKEGLAHLSLAMIGPGDVVLTPTPTYPIHMYSFVIAGGEVRGIELRQDSDFFDDLQR
                     VYRQTFPHPRILVINFPHNPTTAIVDLEFFKKIVAFAKEHHVIVIHDLAYADIVFDGY
                     KAPSFLQVPGAKDVGVEFYTLSKAYNMPCWRVGFCVGNREIVGALAKIKSYLDYGIFQ
                     PLQIASVIALNGPQDCVKETV"
     gene            1184..1897
                     /locus_tag="VI359_03605"
     CDS             1184..1897
                     /locus_tag="VI359_03605"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY6973372.1"
                     /translation="MARLGVNVDHVATLRQARGGTDPDPLGAAVLVELAGADGIVVHL
                     REDRRHIQDRDVQLLREIIRTKLDLEMAADDTMAKIALTVKPDLVTLVPEKRQELTTE
                     GGLDVLAHKDRIQSIITLLHDGGIPVSVFIEPDLAQIKAAHKISADFIELHTGRYANA
                     KRSKEADAEFEAITQSAKLAYKLGMGVNAGHGLNYQNVKRLTHIPEIVEYNIGHSIIS
                     RAILVGLDQAVREMKALLV"
BASE COUNT          471 a          559 c          530 g          465 t
ORIGIN      
        1 gactgtttct tttacgcaat cttggggccc attgagagcg atgacgctcg caatctgcaa
       61 cggttgaaag atgccgtagt ccagatagct cttgatcttg gccagcgccc cgacgatctc
      121 acgattgccg acgcaaaatc cgacccgcca gcacggcatg ttgtaggcct tggacaaggt
      181 gtagaactcg acgccgacgt ctttcgcgcc tggcacttga agaaaactgg gggctttgta
      241 cccgtcgaat acgatatccg cataggccaa atcatggatc acgatcacat ggtgttcctt
      301 ggcaaacgcc acaatttttt tgaaaaactc aagatccaca atcgcggtcg tgggattgtg
      361 cggaaaatta atgacgagaa ttctgggatg cggaaacgtt tgccgataga cgcgttgcag
      421 gtcgtcgaaa aaatcgctgt cctgccgcag ctcgatcccg cgaacttcgc ctcccgcaat
      481 cacgaagctg tacatgtgga tggggtacgt cggagtcggc gttaagacca catcgcccgg
      541 tccgatcatc gccagagata aatgggccag accttccttg gaaccgatcg tgacgatcgc
      601 ctcggtttca ggatcgagat ccacgtcata attgcgttta taccacgcag caatggcatg
      661 gcgtagcttg gttatcccgc gagaggcaga ataacgatga ttctttccct tcctcgcagc
      721 ttcaatcatc ttttccacga tgtgattcgg tgttggctgg tcgggatttc ccatgccgaa
      781 atcgatgatg tcttcgccgt ggtgacgagc ctcgagcttg aggctctgga cctgcgcaaa
      841 gacataggga ggaagtcgct tgagccgata aaaattatct gacaatccca tggatgtctt
      901 ctgtcagacc attcactttg ggagggaaga gccgcccatc gctggtggtg ttatcgtatg
      961 aaacgctatt ctaattgtga ggatgctgca gagtcaaatt atcttgtgat tctgtggggt
     1021 tctgcgtctg agcctacatg cgcttccagc aaaactaggg tccgaaacca gctctccgct
     1081 ccttgcccct ccaagccggt ttctgttaca acagcggcaa ccattccgtc ttgcactcaa
     1141 cctcgattct ccttcgctag atcagctcca gctggaggct actgtggcta gactcggtgt
     1201 caatgtcgac catgtggcca ccctgcgcca agcccggggc ggaacagatc cggacccctt
     1261 gggtgctgcg gtgctcgttg aattggccgg ggcggacggc attgtcgtcc accttcggga
     1321 agatcgtcgt catattcaag accgtgacgt gcaactactc cgggaaatta tccggacaaa
     1381 actcgatctg gaaatggcag ccgatgacac catggcgaaa attgcgctca cggtcaaacc
     1441 cgatctcgtg acactcgttc cagaaaaacg ccaagagttg acgactgagg gtggcctcga
     1501 tgtccttgcc cataaggacc gcattcagtc cattattaca ctgctgcacg acggcggcat
     1561 tcctgttagt gtgttcatcg agcctgattt agctcagatt aaagctgccc ataagatctc
     1621 cgctgacttc atcgagcttc acacaggccg ctatgcgaac gccaaacggt cgaaggaggc
     1681 cgacgccgaa ttcgaggcga tcacgcaaag cgcgaagttg gcctacaagc tcggcatggg
     1741 tgtcaacgcc ggccatggct tgaattacca gaatgtgaag cgtctgactc acatcccgga
     1801 aatcgtggaa tacaatatcg gccacagcat catctcccgc gccatactgg ttggtctcga
     1861 tcaagccgta cgagagatga aagcattgct cgtctgagca gacatccatc gttccccttt
     1921 ccgcaacgtc cactgcgagt tgatctaccg tgattgtgcg agccaccaga tgaaaatcgt
     1981 gactgcgacc cagatgcgga cgctggacca ccgcacgatt accga
//
LOCUS       DATDNW010000145         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_87682,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000145 DATDNW010000000
VERSION     DATDNW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87682"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..350)
                     /locus_tag="VI359_03610"
     CDS             complement(210..350)
                     /locus_tag="VI359_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973373.1"
                     /translation="MDFAHMIFHWFTQNPFTAASVVFGAGVVAVTLYTYLYDYETLPT
                     RD"
     gene            complement(576..872)
                     /locus_tag="VI359_03615"
     CDS             complement(576..872)
                     /locus_tag="VI359_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973374.1"
                     /translation="MRTYRIIKLTLLIIASLVLMTGLPSLSTQAADQSLEGAIIVPLP
                     GEPGLVAAGAVEDTLSACLARIPSDASAGQRMLAEQSCQQNEATRTMTQAAPKF"
     gene            complement(1172..1399)
                     /locus_tag="VI359_03620"
     CDS             complement(1172..1399)
                     /locus_tag="VI359_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973375.1"
                     /translation="MTCSRCDGLRRIIKMKDVVSGESTSGWQCLLCGDVIDSVIVANR
                     KGHAEPASNRARLPGSVPAGTGRSKFGKARY"
     gene            complement(2313..>2504)
                     /locus_tag="VI359_03625"
     CDS             complement(2313..>2504)
                     /locus_tag="VI359_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY6973376.1"
                     /translation="ADCSLEAGEAFRRLRHETLTAERLALIDLRNNGTISDEVLHRLE
                     YELDIEALRQGIGERRLTR"
BASE COUNT          554 a          746 c          659 g          545 t
ORIGIN      
        1 gtgttgaccc gatctctccc ttgaggtcca gaccattcct tcggctccgc tcgcgtctcc
       61 cgaaaatgaa tgggacgaaa cccgcacgaa ctccttagac aacacgatca gatgaacctg
      121 atgccctggg tgggcctcga cagactcatc tgagttcctc atcgtcaacg cttcggagtc
      181 cacaccaaag acactttgtg ctttcaggcc tagtcccgag tgggcagtgt ttcgtaatcg
      241 tacaagtatg tgtacaacgt caccgcgaca acgccggcgc caaagacgac ggacgccgcc
      301 gtgaagggat tttgcgtgaa ccagtgaaat atcatgtgtg cgaaatccat aattgtcctc
      361 tctgtcgtca ggcacccaca cataacgatg tgtatgtcaa cggaacgggt ccgatcggcg
      421 tgggttcatc cgttttcgga cctactttct tggagccgcc tatctgatcc ttggcttctc
      481 ctcagtgaag gacgcccaca tatcgcctct ccgccgtcgg cccctcggcc ccatatcgga
      541 ttggatgccc tactcccttt cgccgaattg tccactcaga acttgggcgc agcttgggtc
      601 attgtcctgg tcgcctcgtt ctgttgacag ctctgttcag ccagcatgcg ctgaccggca
      661 ctggcatctg agggaatccg agccagacag gccgagagtg tgtcctcgac tgcgcccgcc
      721 gccaccaggc caggctctcc cggcaagggg acaataatgg ctccttccaa agattgatcc
      781 gccgcctgtg tggacagcga ggggagtccc gtcattagca cgagtgaagc aataatgagg
      841 agtgtcagtt taatgattcg ataggttctc atggtgcttt cctcccttgt gttaagttga
      901 agtggtcatg ccgccttctt catggtcgaa caaaaagctt cgtcaggttg cttcatgtcg
      961 attgccgtag ttgactcgga tgccatcatt gcccaacacg tcgcggcaag agcggcgatc
     1021 atcccaccga gggcaagaga tccatctatc attgaattta tagagttcat ggtcggcacc
     1081 tccttagtaa ggacctcact ctcgtgcggt cgtcagtcgt ttgcgatttc ctatgcgata
     1141 cactcctact caaaaccaat tgaatatcga gtcaatatcg agctttcccg aattttgatc
     1201 gccctgttcc cgccggcaca gaccctggaa gacgagcccg gttcgacgcc ggttctgcat
     1261 ggcctttccg attcgcgacg atgacggaat cgatcacgtc accacacaac aggcattgcc
     1321 agccggaggt ggattcgccg ctcaccacgt ctttcatctt aatgatccgc cgtaatccgt
     1381 cgcagcgtga acaggtcatg ccgatcactt ggcacccttc ccccgctcga caccgtcttc
     1441 agcgccgaga tcttgttcga tccagcgaac cgactaaaga cgacgcaggc aaacaggaaa
     1501 ttgtccttgt gccgccctgg cacgagatac acgacggcca cgggacctgg tggttaccca
     1561 tacgaccgat cacgagtcga aaaggcccga ccgaggagcg gcggagactg gggaatcaga
     1621 cgcgcaggga gaaatcagaa ggcgcgaggg ataggacagt cagcgaggct caggacttga
     1681 ctgacccacc ctacaggtag aaggcagtga tgtcaacgag cgtggggagt atctgaaccg
     1741 taatgtggtg attctgttca gttttatatt tttcctgaca gtaaccccga aagaggagat
     1801 gggaggcctc ctatcaggta agactggcat gtaatcagtg tgggccccac ccactccgcg
     1861 cctgattctt gtaatcgcga tgaaccactt agggcgcatg cgaccaaagt ccgtacggcg
     1921 atgcaggcga agtgcgcatt tagaatcgag cacaacagca ccactcggtc cacgccagaa
     1981 gaccacccag tgccagaatg cgcgaccgcg cactcgatgc catttgatcg ccagcagcgc
     2041 aaggcgcggc aaggcccgcc acgtcgtaaa tctcgtctca gtcctggctg catggacacc
     2101 aaactgtcga agcaatcggc gtacgtaacc ggtttcagac cacaagcgcg gatcatcggc
     2161 gaaaatgtcc agccggttcg ccacccgctg ggcctgtcga taactcacgc cggcgaccgt
     2221 cgcgacactg gcgatcccac agccggtccg ttcttcttga ataacaggtt tcatgtttca
     2281 tgcgctttcg tcggctggcc tacgagcaga tatcatctcg tcaggcgtcg ttcaccaatt
     2341 ccctggcgaa gcgcttcaat atcgagttca tattccaggc gatggagcac ctcgtcactg
     2401 atcgtgccgt tattccgaag atcaatcaag gcgaggcgct ctgccgtgag cgtctcatgc
     2461 cgcaaccgtc ggaaggcttc gcctgcctca agagagcagt ccgc
//
LOCUS       DATDNW010000146         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_837374, whole genome shotgun sequence.
ACCESSION   DATDNW010000146 DATDNW010000000
VERSION     DATDNW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837374"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="rplB"
                     /locus_tag="VI359_03630"
     CDS             <1..639
                     /gene="rplB"
                     /locus_tag="VI359_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY6973377.1"
                     /translation="RTIDFRRDKVGIPAKVTALEYDPNRTARIALLQYADGEKRYIVA
                     PVGLQVGDVVQSGPDSEVRPGNALPLANMPLGTTIHNLELKPGKGAQLIRSAGGFAQV
                     MGRDGNYVQVRLKSGEMRRVLATCLATVGQVGNVDHENVTVGKAGRSRWKGRRPHVRG
                     VVMNPVDHPHGGGEGKSGQGNPHPVSPWGLPTKGYKTRNNKRTEKFRIARCK"
     gene            649..936
                     /gene="rpsS"
                     /locus_tag="VI359_03635"
     CDS             649..936
                     /gene="rpsS"
                     /locus_tag="VI359_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY6973378.1"
                     /translation="MPRSVTKGAFVDDHLLKKVEQMNQTKDRKLIKTWSRRSTVVPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVPLK"
     gene            959..1312
                     /gene="rplV"
                     /locus_tag="VI359_03640"
     CDS             959..1312
                     /gene="rplV"
                     /locus_tag="VI359_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY6973379.1"
                     /translation="MTEARAILRHVRVAPRKARPVIDMIRGQQVPLALAMLKHTPRHA
                     ARVVEKILRSAVANAEQKEMGDSDSMWVSKAFVNCGPTYKRFRARSMGRANAIQKRTS
                     HITIIVSGAGVQEKK"
     gene            1372..2043
                     /gene="rpsC"
                     /locus_tag="VI359_03645"
     CDS             1372..2043
                     /gene="rpsC"
                     /locus_tag="VI359_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY6973380.1"
                     /translation="MGQKTHPIGYRLGYNYTWSSRWYADKDYAKLLHQDIKIRKMVKQ
                     RLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKASLEKEYAGQVYINV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMIAGRLGGAE
                     IARTEWYREGRVPLHTLRAEIDYGFAEAHTTMGQIGVKTWIYKGELLPVQPLKPESAF
                     ERRLG"
     gene            2058..>2425
                     /gene="rplP"
                     /locus_tag="VI359_03650"
     CDS             2058..>2425
                     /gene="rplP"
                     /locus_tag="VI359_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY6973381.1"
                     /translation="MLAPKKVKFRKMMKGRMTGKAYRGGHLTLGEFGLKALEPGWVTS
                     RQIEAARIAITRYVKRGGQVWTRVFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMDGVTPDIAHEAFRLAS"
BASE COUNT          583 a          598 c          798 g          446 t
ORIGIN      
        1 cgcacgatcg attttcgtcg tgacaaggtg ggcattccgg cgaaagtgac ggcgctggaa
       61 tatgatccca accgcacagc acggattgcc ctcctgcagt atgcagacgg tgaaaagcgg
      121 tacatcgtgg cgccggtcgg attgcaggtt ggtgatgtgg tgcagtcggg tcctgattct
      181 gaagtgcggc ctggcaatgc cctaccgctg gcgaacatgc ctctgggtac gacgattcac
      241 aatctcgagc tgaagccagg gaaaggggcg cagctcatcc gcagtgccgg cggctttgcg
      301 caggtgatgg gacgcgacgg caactatgtc caggtccggt taaagtcggg cgagatgcgg
      361 cgggttttag cgacatgtct ggccacggtc ggccaggttg gcaacgttga tcatgaaaat
      421 gtcacggtcg ggaaggcggg gcgttcgcgc tggaaaggac gacggccgca tgtgcgcggt
      481 gtcgtcatga atcccgtcga ccatcctcac ggtggcggcg aaggaaaatc cggacagggg
      541 aatcctcacc ccgtgtcacc ctggggcctg ccgacgaagg gctataagac tcggaacaat
      601 aagcggacgg aaaagttccg tatcgcgcgg tgcaagtagg agtagccgat gccaaggtca
      661 gtgacaaaag gcgcgtttgt ggatgaccat ttgctcaaaa aggtcgagca aatgaatcaa
      721 actaaggacc gcaagctcat taagacgtgg tcgaggcgtt caaccgtggt tccggacatg
      781 atcggtcaca cctttgcggt gcacaatggg aagaagttca ttcctgtctt tgtgaccgag
      841 aatatggtcg ggcacaagct cggcgaattc gcgccgacgc ggttctttaa gggacatgga
      901 caagcaaaga cggaaaaggc cgtcccgctc aagtaggaga ctgcagcagg gtttcatcat
      961 gacagaagca cgagccattc ttcgacacgt tcgggtcgcc ccgcggaagg cgcggcctgt
     1021 gattgatatg attcgcggac aacaggttcc tctggcattg gccatgctca agcacacccc
     1081 gaggcacgca gctcgggtgg tggagaaaat tcttcgctcc gccgtagcga atgccgaaca
     1141 gaaggaaatg ggcgacagcg attctatgtg ggtgtcgaaa gcgtttgtga attgcgggcc
     1201 gacctataag cggtttcgcg cgcgatccat ggggcgggcc aacgcgatcc agaaacggac
     1261 cagccacatt acgatcattg tgtcaggggc aggcgttcag gaaaagaagt agcacgcgac
     1321 gattatcgcc gggaacaaca ccgttccccg tggaagtaag ggtggaacag tatgggtcag
     1381 aagacacatc caatcgggta tcgtctggga tacaactata cgtggagctc tcggtggtat
     1441 gccgataagg actacgccaa gctgctgcat caggacatca agattcggaa gatggtcaag
     1501 cagcgcttgt accacgccgg agtcgcgaag gtggagattg agcggtcggg tgatcaaacc
     1561 cgcgtgatta tccacacggc caggccgggg atcattatcg gacggaaggg agcggaagtg
     1621 gacaagctca aggcctcgct cgagaaggaa tacgcggggc aagtctatat caatgtcaaa
     1681 gagatcaaga aaccggaatt ggatgcgcaa ctggtcagcg agaatgtcgc cacccagttg
     1741 gaaaagcgcg tagccttccg tcgagccatg aagcgcagcg tgcagtcggc gcttcgcctc
     1801 ggagcgcaag gcatcaagat catgattgcc ggtcgtctcg gcggtgcgga aatcgcaaga
     1861 acggagtggt atcgggaagg tcgtgtgccg ctccacacgc ttcgggccga gatcgattat
     1921 ggatttgcgg aggcgcatac cacgatgggg cagatcgggg tgaagacgtg gatttataag
     1981 ggagagcttc tccccgttca gccactcaag ccggaatcag cgttcgagcg tcggcttgga
     2041 taacggaaga ggcgactgtg ttagcaccaa agaaagtcaa attccgcaag atgatgaagg
     2101 gccgcatgac aggcaaggcc tatcgtggcg gacacctcac tctgggagag ttcggcctca
     2161 aagccttgga gcctggctgg gtgacgagcc ggcaaatcga agcggcgcgt attgcgatca
     2221 cccggtacgt gaaacggggc gggcaagtgt ggacaagagt ctttcctgat aagccgatta
     2281 cgaagaaacc ggcggagact cgtatgggaa agggcaaggg aaatcccgag tactgggtgg
     2341 cggtcgtgaa gccgggccgg atcctctatg agatggatgg cgtgacgccg gacattgcgc
     2401 atgaagcgtt caggctggcg tcgca
//
LOCUS       DATDNW010000147         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_433177, whole genome shotgun sequence.
ACCESSION   DATDNW010000147 DATDNW010000000
VERSION     DATDNW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433177"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VI359_03655"
     CDS             <1..1408
                     /locus_tag="VI359_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY6973382.1"
                     /translation="TVRLELANKPGMFAKVAALLADEGANLGAVDIVSATADRMVRDI
                     TFDVQDETHGDRVLARLGRLPHVTVLSASDRIFLLHLGGKIHVQSKLPITTRNILSMV
                     YTPGVGRVSQAIAKDPSKAYAFTTKCNSVAVITDGSAVLGLGNLGPEAALPVMEGKAM
                     LFKELAGIDAWPICLNTQDPDQIVQTILAIAPGFGAINLEDIGAPRCFEIERRLRAAL
                     DIPVMHDDQHGTAVVILAALSNALKLTGKKMEEVRVVVNGLGAAGTACCRMLLAAGVS
                     HLLGCEPQGIVLHGEPDQLRACRTNLSSCISRDRAHGSLQDALKGADVFIGLSVGNIL
                     SADDLNLMAGDRIVFAMANPDPEVPPQLGSSHCRIFATGRSDYPNQINNALAFPGIFR
                     GALDVQAREINETMKLAAATALAESVPPSVLSEDYIIPSVFDKEVVPRVAKAVATAAR
                     ETGVARRRTKLSDDFATS"
     gene            1483..2118
                     /locus_tag="VI359_03660"
     CDS             1483..2118
                     /locus_tag="VI359_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY6973383.1"
                     /translation="MPFSASKFIKFRNGMQRRITSLRAKADDSLEVAVDTMMEDRVDS
                     FFRVEQGLEEIVKSLVLIEEELEQIRDLSGAMRLESRLEFVEDRWDDFDSEIRERPRR
                     RRRKINLADMLKAASGGGGDLSQGSSSVNNAVDAYAIMGVEFGSPLADVTTAFRQKAK
                     QLHPDANNGDRSSEPELRRMLEAYQFLKEYLSLSNVEPPSRPPNTTYSPTE"
     gene            2123..>2607
                     /locus_tag="VI359_03665"
     CDS             2123..>2607
                     /locus_tag="VI359_03665"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY6973384.1"
                     /translation="MPTSAPIQYITDRRGLESLCRTLDQSPRLALDTEFVGEETFVPR
                     LELIQVATESTAAIIDFPAVQADGSLDAFWHLVCRSDVEKIVHAGRQDLDLFAAHAGQ
                     IPKPFFDTQIAAAMVGYGAQVAYANLVHRLHGTKLDKAHTFTNWSARPLSRAQLTYAL
                     G"
BASE COUNT          574 a          722 c          726 g          585 t
ORIGIN      
        1 gactgttcgc ctcgaactgg ccaataaacc cggcatgttt gcaaaggtcg ccgcgctgct
       61 tgctgatgag ggcgcgaatt tgggagcggt cgatatcgtt tccgcgacag cggaccgcat
      121 ggtccgtgac attactttcg atgttcagga cgaaacgcac ggtgacagag ttctagcgcg
      181 attggggagg ttacctcacg ttaccgtcct ttcagcatct gacagaattt ttctcctgca
      241 tctaggcgga aaaatccacg ttcaaagcaa actgccgatt acgactagaa atatcctgtc
      301 gatggtgtat acccctggtg tgggacgcgt gtctcaagcc atcgccaaag atccttcgaa
      361 agcctacgcc ttcacaacta agtgtaatag cgtcgctgtg attaccgatg gatccgctgt
      421 gttggggctc ggtaatctgg gtccggaggc tgccctccca gtgatggaag gcaaagcgat
      481 gctgttcaaa gagctcgcgg gcatcgatgc gtggcctatt tgtctcaaca cgcaggatcc
      541 tgaccagatc gtgcagacga ttctcgcgat cgcccccggc tttggtgcaa ttaacctcga
      601 agatatcggt gccccacgct gtttcgaaat cgagcggcgg ttacgcgccg ccctggatat
      661 cccggtgatg cacgacgatc agcatggcac ggccgtggtc attctcgctg cgctctcaaa
      721 cgccctgaaa ctcaccggga agaagatgga agaggtccgc gtggtagtga atggtcttgg
      781 agccgcagga accgcctgtt gcagaatgct cttagccgca ggcgtgtcgc atttgctggg
      841 gtgtgaaccg caaggaattg tgttgcatgg tgaacctgac caattgcggg cgtgccggac
      901 gaatctgtcg agttgcatca gccgtgatcg ggcgcacggc tctcttcaag acgccctgaa
      961 aggagccgac gtcttcatcg gactttctgt cgggaatatt ctctctgctg atgacctgaa
     1021 tctgatggcg ggggatcgaa tcgtatttgc catggctaat ccggaccccg aagtccctcc
     1081 acagctcggc agctcccact gtcgaatttt tgcgaccggg cgttcggatt atcccaatca
     1141 gatcaacaat gccctcgcct ttcccggtat ctttcgcggc gctttggacg tgcaagctcg
     1201 agaaatcaat gaaacgatga agctggctgc ggcgaccgcg ctcgctgaat cagttccacc
     1261 ttcggttctc agtgaagact atattattcc cagtgtattt gataaagaag tcgttccacg
     1321 ggtcgcaaag gccgtcgcca cggctgcacg ggagaccggc gtggccagac gtcggaccaa
     1381 acttagcgac gacttcgcca caagctaacc ctctccgtcc ttccttctgc catatggccg
     1441 cgctgtgcta caatgcgcat gttcccacgg gggcaatcac cgatgccttt ctccgcgagc
     1501 aagttcataa agtttcgaaa cggcatgcag cggaggatta cttccctccg cgccaaagcg
     1561 gacgacagtc tcgaagtggc cgtcgatact atgatggaag atcgcgtcga cagttttttc
     1621 cgcgtcgagc aagggctgga agaaattgtg aagtctctgg tgctgatcga ggaagaacta
     1681 gaacaaattc gcgatctctc tggtgccatg agattggagt cccgcctgga gttcgtcgag
     1741 gaccgttggg acgacttcga cagtgaaatc cgggagcggc cgcggcgacg gcgacgaaag
     1801 atcaatctgg ccgacatgtt gaaagccgcg agtggaggag gtggcgatct ctcccaggga
     1861 tcgagctcgg tcaacaacgc agtggacgcc tatgcgatca tgggcgttga attcgggagt
     1921 ccactggccg acgttaccac ggcctttcga cagaaagcga aacagcttca tccggatgca
     1981 aacaacggag atcgtagctc tgagccagag ttgcggcgga tgctggaggc gtaccagttt
     2041 ctcaaggagt atctgagtct cagcaatgtc gaacctcctt ctcgaccacc gaacacgacg
     2101 tacagtccga ccgagtagct gcatgcccac ttctgctccg atccagtaca tcaccgaccg
     2161 tagaggactc gagtcactat gcagaacgct cgatcagagt ccgcggcttg ccctcgacac
     2221 ggaatttgtt ggggaagaaa cctttgtccc ccgcctcgaa ttaatccaag ttgcgacgga
     2281 aagcactgct gctatcattg attttccagc cgtccaagcc gacggatccc ttgacgcttt
     2341 ctggcacctt gtctgtcgtt ctgatgtcga gaaaattgtt cacgcagggc gacaagacct
     2401 cgatcttttt gcggctcatg ccggccagat tcctaaaccg tttttcgata cccagattgc
     2461 agctgcaatg gtgggctatg gcgcacaagt ggcttatgcc aatcttgtcc atcgcctaca
     2521 tgggaccaaa ctagacaagg cgcatacgtt tacgaattgg agtgcccggc cactctcacg
     2581 tgcgcaactc acatatgcgc tggggga
//
LOCUS       DATDNW010000148         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1041494, whole genome shotgun sequence.
ACCESSION   DATDNW010000148 DATDNW010000000
VERSION     DATDNW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041494"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="lysS"
                     /locus_tag="VI359_03670"
     CDS             <1..1126
                     /gene="lysS"
                     /locus_tag="VI359_03670"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY6973385.1"
                     /translation="TKTNEFTIEVHGLTFLSKALRPLPEKWHGLTDVETRYRQRYVDL
                     IANPEVHGIFAVRSKIIAGIRSFLIERGFLEVETPMMHPIPGGATAKPFVTHHNALGV
                     DLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADYHDL
                     IILTEELITALATQILGKTVIEYQGKELNLTPPWRRWSYHQAILEVNRLDASVLQDRD
                     KAMAAAKQLNVPVDPKWPLFNIVNEIFEETVEPNLQQPTFITDYPIEISPLARRKDAN
                     PSLTDRFELYIAGREIANAFSELNDPLDQRERFEGQAAQRAAGDEEAHLVDEDFLRAL
                     EYGMPPAAGEGIGIDRLIMLFTDQASIRDVVLFPQLRPEK"
     gene            1133..2413
                     /locus_tag="VI359_03675"
     CDS             1133..2413
                     /locus_tag="VI359_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY6973386.1"
                     /translation="MPLPYEIFVGLRYLRAKRRNRTISLNTFVSIAGITLGVAALIGT
                     VGIMTGFKEDIQAKILGTTAHIIVQDRMKDGMADYDAVANKVQQVPDVVAATPFVLKQ
                     VLLTSQTGVQGIVIRGIDPKREGTVTELGKNLAAGTLTDLQSPIKVKQSPPDNPTGPP
                     VETEKPGIILGKELALRLGVFMGDTVNVVSPPAGPVSAMGMVPKIRTFAVAGIFQSGM
                     YEYDSSLAYIDLGEAQKFFNLGATATGVEIKVTDVFRAADIARSVEQSLGFAYGARDW
                     MQMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATS
                     TSIRRVFMLNGLIIGLTGTTIGIPLGYAFLWLIQTFWTFDPTVYYISRIPVHVLASDV
                     LLVAGSAILISFFATVFPARQAARLEPAAALRYE"
     gene            2477..>3066
                     /locus_tag="VI359_03680"
     CDS             2477..>3066
                     /locus_tag="VI359_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6973387.1"
                     /translation="MASAMITINDLYKSFKIGERDLTVLKGINLEIRRGELLAIMGAS
                     GAGKSTLLHIVGLLDRPTQGTVSFEGQNLFQLPEATQAEFRNKRIGFVFQFHHLLPEF
                     TALENASMPALIQRRSPADVEADATALLHEVGLGERLHHKPGELSGGEQQRVAVARAL
                     MQRPDLVLADEPTGNLDTHTGEALFALMRDLNKSRGP"
BASE COUNT          678 a          896 c          845 g          647 t
ORIGIN      
        1 gacgaagaca aatgagttca ccatagaagt tcacggtctc acgttcttga gcaaagccct
       61 tcgaccgctt ccggaaaagt ggcacggcct gaccgatgtg gaaacacgct accgccagcg
      121 ctacgtcgat ctcattgcta atccggaggt ccacgggatc tttgcggtcc gtagcaaaat
      181 catcgccggg attcggtcgt tcctcattga gcgagggttt ctcgaagttg aaacgccgat
      241 gatgcacccg atccccggtg gggccaccgc gaaaccgttc gtgacccacc acaatgcgct
      301 cggcgtcgat ctctacctcc gtatcgcgcc ggaactgtat ttgaagcgat tgatcgtcgg
      361 aggctttccg cgggtcttcg agattaatcg caattttcgg aatgaaggga tctcgactat
      421 ccacaatcct gaattcacga tgctggagtt ctacgtctcc tatgcggact accacgatct
      481 gatcattctc acggaagaat tgattaccgc cttggccacc cagattctcg ggaagaccgt
      541 gatcgagtac cagggcaagg agctcaacct gacgccgccg tggcgacgct ggtcctacca
      601 tcaggcgatc cttgaggtga atcggctgga tgcgtcggta ctccaggatc gggacaaagc
      661 catggcggcg gccaagcaac tcaacgtgcc ggtggatccc aagtggccac tcttcaatat
      721 tgtgaatgag atcttcgagg aaacggtcga gcccaatctg cagcaaccga cctttatcac
      781 cgattacccc atcgaaattt ctcccctggc gcggcggaag gatgccaatc cctcgttaac
      841 cgatcgcttc gagctgtaca tcgccggtcg tgagatcgcc aatgcctttt ccgagttgaa
      901 cgatccgctc gaccagcggg aacgatttga gggacaggca gcgcaacgag cagccggcga
      961 tgaagaggcc catctcgtgg atgaagactt tctccgcgcc ctggagtatg gcatgccgcc
     1021 ggcggcgggc gaaggaatcg gcatcgaccg cctcatcatg ctattcaccg atcaggcgtc
     1081 gattcgagac gtcgtgctct ttccgcaatt gaggccggag aaataattcc gcatgcccct
     1141 cccctatgaa atcttcgtcg gcctccgtta cctgcgcgcc aaacggcgga atcgaaccat
     1201 ctcgctgaat acattcgtct cgatcgccgg cattaccctc ggcgtggccg ccctgatcgg
     1261 aaccgttggc atcatgactg ggttcaagga agacattcag gctaagattt tgggcaccac
     1321 cgcccatata atcgtccagg accggatgaa agacgggatg gccgactacg acgccgtagc
     1381 caacaaggtt caacaggtgc cggacgtggt cgcagcaacg ccttttgtgt tgaaacaggt
     1441 gctgctgaca agccagaccg gtgtgcaagg gattgtgatc cgcggcatcg atccgaaacg
     1501 ggaaggtact gtcaccgaac tcgggaagaa ccttgccgca ggcaccttga ctgacttgca
     1561 gagccccatc aaagtcaaac agtctccccc ggacaatccg accgggcccc cggtcgagac
     1621 ggaaaaaccc ggcatcattc tcgggaaaga gctggctctg cgactcgggg tattcatggg
     1681 cgatacagtc aacgtcgtgt ctccgcctgc cggccccgtc agtgcgatgg gaatggtacc
     1741 caagatcaga acgtttgcgg tcgcaggcat tttccagtct ggcatgtacg aatacgattc
     1801 ctcgctggcc tacatcgacc ttggcgaagc gcaaaagttt tttaaccttg gggcgaccgc
     1861 cacgggagtc gaaatcaagg tgaccgatgt cttccgcgcc gcagacattg cccggtcggt
     1921 tgagcagtcg ctcggttttg cctatggggc cagagattgg atgcaaatga accgaaattt
     1981 gttttctgca ttgaagctag aaaaaacgat gatgttcttg ctcttggtcc tgatcaccat
     2041 cgtggcttcg ttcaacatcg tgagcacctt gacgatgatc gtgaccgaga agcaaaagga
     2101 aattgcaatc ttgaaagcaa tgggcgcgac cagcaccagt atccggcggg tcttcatgtt
     2161 gaatggcctg atcatcggcc tcaccggcac aaccatcgga attccgctcg gctatgcgtt
     2221 cctctggctg attcagactt tctggacctt cgacccgacc gtctactaca tctcccgcat
     2281 tccggtccat gtcttggcat ccgacgtttt gctcgtcgcc ggctccgcca tattaataag
     2341 ctttttcgcc acagtgtttc ccgctcgcca agcggccagg ttggagcccg cggcggcgct
     2401 gcgatatgag taaccaccaa ctcacgatcc agcccccagt tgacggccgc gaacaatgtc
     2461 cagacgcccc gcgtcgatgg cttcggctat gatcacgatc aacgacttgt acaagagctt
     2521 caagatcggc gagcgcgatt tgaccgtgct caaggggatc aatcttgaaa ttcggcgagg
     2581 cgaactgctc gccatcatgg gcgcatcggg tgccggaaag agcacgctcc tgcatatcgt
     2641 cggtctgctg gatcgcccga cacaggggac agtctcgttt gaaggacaga atctctttca
     2701 gcttccggaa gcgacgcagg cggagtttcg caacaagcgg atcgggtttg tctttcagtt
     2761 tcatcatctc ttgccggaat tcacggctct cgagaacgca tccatgcccg ctttaattca
     2821 acggcggtca ccggctgacg tcgaagccga cgccaccgcg ctgctgcacg aagtgggatt
     2881 gggcgaacgg ctgcatcaca aacccggcga attgtctggc ggtgagcagc aacgagtggc
     2941 cgtcgcacgg gcgttgatgc agagaccgga cttggtgctg gcggacgaac caacgggcaa
     3001 tctcgacacg cacacaggcg aagctctttt tgccttgatg cgagacctca ataaatcccg
     3061 cggacc
//
LOCUS       DATDNW010000149         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_433236, whole genome shotgun sequence.
ACCESSION   DATDNW010000149 DATDNW010000000
VERSION     DATDNW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433236"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VI359_03685"
     CDS             complement(<1..689)
                     /locus_tag="VI359_03685"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY6973388.1"
                     /translation="MTLKALPLGVIGMALALAGLIGYSLAPDALWLVTLCESLALVCL
                     IAFVVVHFETLKTFSARRSTRMGANSLVIVLLVVGILAIGNFLAARHSLRWDFSENQN
                     FTLAPQTYRVLRSLSHDVKVTVFTREKDAGFQSYKERLDSYRQASPKLTVEFIDPERH
                     PKVAQSYGITRTDTAIFESGSQTVRVTSPSEVELTGAVLRVSKDNKKRILFLEGHGER
                     GLDDKERTGYS"
     gene            complement(686..1456)
                     /locus_tag="VI359_03690"
     CDS             complement(686..1456)
                     /locus_tag="VI359_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6973389.1"
                     /translation="MTPVATIVSKELRSYFVSPVVYVVGAVFLLIIGLLSYLYVVFAG
                     AQAIQLMQMQGGMAQLNLNDLVFRNLFSSIRFVLLLTLPILTMRLFAEERKLRTFEFL
                     MTSPIGINEIVAGKFMSVFLVFLGLLGLTGLIPVVLSLFSDFDWYPILTGYLGLALLG
                     ALFLSVGVLASALTENQIVAAFLSFGILLLFWLLAGLGSLLGDTALGQTISYLSFMEH
                     FDHLVRGLIDTKDLVYFFSGLVLMLFLTHRAVEAARWR"
     gene            complement(1453..2454)
                     /locus_tag="VI359_03695"
     CDS             complement(1453..2454)
                     /locus_tag="VI359_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6973390.1"
                     /translation="MIDVQQVTKRYGQHTALDRVTFRVAKGEILAFLGPNGAGKTTTM
                     RILTCFMPATEGSASVAGFDCANQPSEVKRRIGYLPETPPVYQELTVSEYLTFVGRLR
                     GLTGKQLSAAMDREVQRLGLGQVRDRLIGNLSRGYRQRVGLAQALLHDPEVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIISGGRIVAEDTPEQL
                     SARLRHSDKISITLKHPPVDGAEQFKSVAGVQNVFPGGSTGTYLLECSLNHDVREEVA
                     RLAVTQGWGLLELKSISMTLEDVFLQLTRHEDGLSQDGEPSTGRHTSTVPESMEQSA"
     gene            3011..>3195
                     /locus_tag="VI359_03700"
     CDS             3011..>3195
                     /locus_tag="VI359_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973391.1"
                     /translation="MMAMAMNGNRKKAAIRPYLMQFFVVASALVFSAACSWIPKGEIQ
                     LDVGLKDRGVASWYGEQ"
BASE COUNT          746 a          841 c          923 g          685 t
ORIGIN      
        1 aaagagtagc cggtccgctc cttgtcgtcg agtccgcgtt cgccatggcc ttcgaggaac
       61 aggatccgtt tcttgttgtc cttcgaaacg cggagcaccg caccggtgag ttcgacttcc
      121 gatggtgatg tcacgcggac cgtctggctc ccgctttcaa aaatcgccgt gtcggttctg
      181 gtaatgccgt aggactgtgc aaccttcggg tgccgctcag gatcgataaa ctcgacggtc
      241 aactttgggc ttgcttgccg atagctgtcg aggcgttctt tgtatgactg aaatcccgcg
      301 tctttctctc gggtgaagac ggtcactttc acgtcgtggg ataagcttcg gaggacacga
      361 tacgtttgcg gtgcaagggt gaaattctga ttctcggaaa aatcccaacg aagggaatgt
      421 cgagcagcca ggaaattccc gatggcgaga atcccaacaa caaggaggac gatcaccaga
      481 ctgttggcgc ccatgcgcgt cgaccgccgg gcggaaaatg ttttgagcgt ctcgaagtgg
      541 acaaccacaa aagcaatcaa acaaacgagt gccaggcttt cgcataatgt gacgagccag
      601 agcgcgtccg gtgccaggct ataaccgatc aatccggcca gagccagggc catcccaatg
      661 accccgagcg ggagagcctt gagggtcatc gccacctcgc tgcttcaact gcccggtggg
      721 tgaggaatag cattaagacc aagccactga agaagtacac aaggtccttg gtgtcgatca
      781 ggccacgaac taaatgatcg aaatgttcca tgaatgacag gtacgagatg gtctggccca
      841 gcgctgtatc tcccaacaga gatcccaagc cggcgagaag ccagaagagg agcaggatgc
      901 cgaagctgag gaaggccgcc acgatttggt tctccgtcaa ggcagacgcg aggacgccga
      961 ccgagagaaa cagagccccg agcaaggcca gtccgagata gccggtcagg atggggtacc
     1021 agtcgaaatc gctgaacagg gacaacacga ccgggatcaa acctgtgagt cccaagaggc
     1081 cgagaaagac gagaaagacg ctcatgaatt ttcccgcgac gatctcgttg atgccgatgg
     1141 gggaggtcat gaggaactcg aaggtccgca gttttcgttc ttcggcaaag aggcgcatcg
     1201 tcaaaatcgg cagcgtgagc agcaacacaa atctgatgct ggaaaacagg ttgcggaaca
     1261 ccaggtcgtt cagattcagc tgagccatgc ctccctgcat ctgcatcagc tggatggctt
     1321 gtgctccggc gaagacgaca tacaggtatg acagcagccc gatgatcaga agaaagaccg
     1381 ctcccaccac atacaccacc ggcgaaacga agtaggaacg cagctctttc gaaactatgg
     1441 tcgcgacagg cgtcatgcgg actgttccat tgattcgggc actgtggagg tgtgccggcc
     1501 agtcgatggt tctccgtcct gtgacaggcc gtcttcatgc cgcgtaagct ggagaaagac
     1561 gtcttctaag gtcatggaga tcgatttcag ctcgagcagg ccccaccctt gagtgaccgc
     1621 cagtcgagcg acttcttctc gcacatcgtg attaagcgag cattccagaa ggtatgttcc
     1681 tgtgcttcca ccgggaaaca cattctgcac cccggcgacg gatttgaatt gctcagctcc
     1741 atctacggga ggatgtttca gcgtaatgga aatcttgtct gagtgacgga gtcgtgcgga
     1801 cagttgctcc ggtgtatctt ctgcgacaat gcggccgccg ctaatgatca ccacgcgctg
     1861 gcagacggcc gtggcctccg gcagaatgtg ggtgctcagg attactgaat gggatccggc
     1921 caagctcttg atcaattccc ggatttcgat gatctgcttc gggtcgagac cgacggtcgg
     1981 ttcgtcgagg atgaggacct caggatcgtg caagagtgcc tgggcgagtc cgactcgttg
     2041 gcggtaaccg cgagacaggt ttccgataag gcggtctcgt acctggccga ggcccaaccg
     2101 ctggacttca cgatccatcg cagcagaaag ctgctttcct gtaaggcctc gcaatcgacc
     2161 cacaaacgtg aggtattccg acaccgtcag ctcttggtac accggcggtg tctccggaag
     2221 atacccgatt cgacgtttca cttcggaagg ctggttagcg caatcgaacc cggccacgct
     2281 ggctgatcct tcggtggccg gcataaagca ggtcaagatc cgcatcgtcg tcgttttccc
     2341 ggcaccattg gggccgagaa atgcgagaat ttcgcctttc gcaacacgaa aggtcacccg
     2401 atcaagggcg gtgtgttgtc cgtaacgctt ggtgacctgc tgaacgtcga tcatgcgtgg
     2461 gtaattgctg actagatcgt aggaccgctg gcggtgggtt gggacttcat gtacgtataa
     2521 cccgctggta tcttactcag tcatccagag tcagtcaata cagtgagcac aaaactttag
     2581 aggctaggtc tttcccggtc gagtcccttg tgcggcacta attatcagac caaaatcacg
     2641 gccggactcg gcgtgtcgcg ctctaggcca tcgatgcagg aagactatgt tgctaatctc
     2701 taaagtacag cgatgcgctg ttcgcatgga caattcactg gcatggaaag ggtcactcac
     2761 ccttggcagt attctgtcca cgcctcttga ctaaggcgta ggcgtcctta tatacagttc
     2821 tacataggca cttcatcccg cccgttggtt cttatctgtg tgatctttcc cacagaggca
     2881 ttcattttcg actcatccga aggtagggga gggggctacc ggatcggtat tgttgggccg
     2941 gtgctcagcg ttttaaggtg agggcagcca gagaacagtc tgagctgagc acaaggatgg
     3001 ttcaaccaca atgatggcta tggcgatgaa tgggaataga aagaaggcag caatcaggcc
     3061 ctatttgatg caattcttcg tagttgcaag cgcattagtg ttctccgcag cttgctcatg
     3121 gatcccaaag ggtgaaatcc agctcgatgt tggattgaaa gaccgagggg tggcctcctg
     3181 gtatggcgaa cagtt
//
LOCUS       DATDNW010000150         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_219565, whole genome shotgun sequence.
ACCESSION   DATDNW010000150 DATDNW010000000
VERSION     DATDNW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219565"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VI359_03705"
     CDS             complement(<1..1404)
                     /locus_tag="VI359_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973392.1"
                     /translation="MKHDVDPTTKKRVLLIDDEPRIRASLKMVLEPTYEVFQAGDAQE
                     GLEVFRKEAPHLVLLDVILPGTDGLAILQTLRTEDRSTPVIMLTGTKSVKTAVDAMKL
                     GAADYLSKPFEVEELRIVVDKALNSQELEREVKQLRAQVVQRYAFHNLIGKSSSMQDI
                     YGKIEQVADSRTTVLITGESGTGKELVAKALHYNSSRRDRPFVALNCAALPETLIESE
                     LFGHEKGSFTDATARRVGQFELANTGTLFLDEIGDLSPMTQAKLLRVLQEREFTRIGG
                     VQAIKVDVRIIAATNKNIEELVRKNQFREDLYYRINVIALYLPPLRERGEDIPLLAKH
                     FLAKRVEEDGRPHQEFTKEAIELLSRYPWPGNVREMENIIEQAFIWSKGSATITPEHL
                     PNILRNDTRSSSLRDDTLAGRLSLEKAVMEFEREIILDALKRTNYVQTHAANLLGISR
                     RMLKYRMDTLGIGRPDNE"
     gene            complement(1407..>2012)
                     /locus_tag="VI359_03710"
     CDS             complement(1407..>2012)
                     /locus_tag="VI359_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973393.1"
                     /translation="APERKDDADFIREFSKVVIDDVYRIERLIQEILDYARYMEPKLT
                     DEDLNEIVSSCLYFVDVKADRLGIKIEKELAPDLPRVMLDRQQVKQVLLNLLLNAMDA
                     MGERGGRLHVRTHKLMKPGGKIWAQIEIEDTGHGIPEGTLERIFDPFFTTKHESGEHE
                     GTGLGLTIVHQIIQEHQGEIQVKSTVDVGTTFFVNLPALPA"
BASE COUNT          429 a          537 c          518 g          528 t
ORIGIN      
        1 ttcattgtcg ggtcggccaa tcccgagtgt gtccattcga tatttcagca ttcggcggct
       61 aatccccagg aggttggcgg catgggtctg tacatagttg gtccgtttaa gagcgtccaa
      121 gatgatctcg cgctcgaact ccatcacggc tttttcgagg gaaagtcgcc ccgccagtgt
      181 gtcgtcgcga agagaagatg aacgggtgtc attgcggaga atgttcggaa gatgttctgg
      241 cgtgatggtg gcggatcctt ttgaccaaat aaacgcctgt tcgatgatgt tctccatttc
      301 tctcacattg cctggccagg gatagcgact caagagctcg attgcctctt tcgtgaactc
      361 ttggtgggga cgaccgtctt cttcgacacg ttttgcaaga aagtgcttgg ccaggagggg
      421 aatgtcctca ccccgttctc gcaagggtgg cagatagaga gcgatgacgt ttatccggta
      481 atacaaatct tcccggaatt gatttttcct gaccagttct tcgatattct tgttggtggc
      541 ggcgataatt cgcacatcaa ccttaatggc ttgcacaccg ccgatccgtg taaattctcg
      601 ttcctgcaac acacgcagca acttcgcctg tgtcattggg ctcaaatcgc cgatctcgtc
      661 caggaatagc gtaccggtgt ttgcaagctc aaactggccg actcgacggg ctgttgcgtc
      721 ggtgaaggaa cccttctcat ggccgaataa ctccgactcg atgagcgttt ccggaagagc
      781 agcacaattc aacgccacga atggccggtc gcgccgcgaa ctgttgtagt ggagtgcctt
      841 ggcgaccaat tcctttccgg ttccgctttc gcctgtgatg agcaccgtgg ttcggctgtc
      901 cgcgacttgc tcaatttttc cgtagatgtc ctgcatggac gaacttttac cgatcaggtt
      961 gtggaacgca taccgctgta cgacctgcgc acggagttgc ttcacctccc gttcgagttc
     1021 ttgggaattg agtgctttgt cgacgacaat ccgaagttct tcgacctcaa atggcttgga
     1081 gaggtagtca gccgcgccaa gcttcattgc gtcgacagcg gtcttcacgg atttggtccc
     1141 cgtcagcatg ataacgggtg tcgaacgatc ctcggtccgt agtgtttgca ggatcgcgag
     1201 cccatcggtc cccggtagga taacgtcaag gagaaccaaa tgaggtgcct ccttacgaaa
     1261 cacttcgaga ccttcctgtg catcacctgc ctggaacacc tcgtacgtcg gctccaatac
     1321 cattttgagc gaggcccgga tacgaggttc gtcatcgatg agaaggacgc gtttcttcgt
     1381 ggtgggatcg acgtcgtgtt tcatgattag gccggcaatg cgggaagatt aacgaaaaag
     1441 gtggtgccca cgtcgactgt acttttgacc tgaatttcgc cctgatgttc ctgaatgatt
     1501 tgatgaacga tcgtcaagcc gagtccggtt ccctcatgct cgccgctttc atgttttgtg
     1561 gtaaaaaacg gatcaaatat gcgctcaagc gtaccttctg ggataccgtg gcccgtatcc
     1621 tcaatctcga tctgagccca gatctttcct cctggtttca taagcttgtg cgtccgaaca
     1681 tgcaaccggc ctccccgctc gcccattgca tccatggcgt tcaagagcag gtttaaaagg
     1741 acttgcttga cttgctgacg gtccagcatg acacgaggta agtcaggcgc caactctttt
     1801 tcaatcttta tccccagccg atcagccttc acgtcaacga agtagaggca ggacgaaacg
     1861 atctcgttga gatcctcgtc cgtcaacttt ggctccatat accgggcata atccaggatt
     1921 tcctggatta atcgttcaat cctatagaca tcatcaatca ccactttgct gaattctcta
     1981 ataaaatcag catcatcctt gcgttctggg gc
//
LOCUS       DATDNW010000151         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_774248, whole genome shotgun sequence.
ACCESSION   DATDNW010000151 DATDNW010000000
VERSION     DATDNW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774248"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VI359_03715"
     CDS             complement(<1..440)
                     /locus_tag="VI359_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6973394.1"
                     /translation="MRVTEIFHSIQGESSHVGQPCVFVRLTGCPLRCTWCDTEYAFYG
                     GNEQSIDQILTKVDSYGCQLVEVTGGEPLAQTETFQLITRLCDRGYQVLIETSGAIDI
                     SPVDRRAHVILDVKCPGSGMTDRMYWPNLNVLTMKDEAKFVLCDR"
     gene            complement(443..1153)
                     /locus_tag="VI359_03720"
     CDS             complement(443..1153)
                     /locus_tag="VI359_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY6973395.1"
                     /translation="MQGYHDLHPSLVRAATSASSASLAALFDVDNTLIPGEACEVRFF
                     RYLWRRGFVGWLELRRSVTWLIRHASDLSIHPLRERKVYLEGKNPAVIETCAREFFHH
                     EMLGLLSTKGRERLEAHRQSGHQLILVSGAPDFLITPLAEALNVPTLFAARLERREDI
                     YTGEIIPPLPYGKGKRELVVSHAKEKDIELASSFAYGDSPGDIEILELVGYPVVVNPI
                     RGMARTARDRGWPMVNWG"
     gene            1407..1490
                     /locus_tag="VI359_03725"
     tRNA            1407..1490
                     /locus_tag="VI359_03725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1440..1442,aa:Leu,seq:gag)
     gene            1512..>2170
                     /locus_tag="VI359_03730"
     CDS             1512..>2170
                     /locus_tag="VI359_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973396.1"
                     /translation="MTSASATSPRRRTSQSAKSPSIRLLLVDDHEVVRVGLRTVLHNN
                     QGITIVGEADNKAQAAKEAKRLRPDVILMDVRLPDGTGVEACREILSDNPDSRVIFLT
                     SYTDDDSVLAAVLAGAYGYVLKEIDSAALIRAIRTVSTGRSILDPNVTKRALEWIKTV
                     PTADGPSRASSLSPQEERVLALVAEGLTNKEIALSLKLSDKTVKNYLANMFQKLHISR
                     RA"
BASE COUNT          501 a          619 c          595 g          455 t
ORIGIN      
        1 cgatcgcaca agacaaattt cgcttcgtct ttcatagtga gcacgttgag attcggccaa
       61 tacatccggt ctgtcatgcc actgcctgga catttcacat cgagaatcac gtgggcccgc
      121 cggtccaccg gggagatgtc gatagcgccg ctggtctcga tcaacacctg atagcccctg
      181 tcgcagagtc tcgtaatcaa ttgaaacgtc tccgtctgag cgagcggctc cccgccggta
      241 acctccacca gttgacaccc atacgagtcc accttcgtca atatctgatc gatggactgc
      301 tcattcccac cgtagaaggc atattctgta tcgcaccagg tgcaacgcag tggacagccg
      361 gtgagccgaa cgaagacaca gggctgaccc acgtgactcg attccccctg gatactgtga
      421 aaaatttctg tcactcgcat ggtcagcccc agttcaccat tggccacccg cgatcacgcg
      481 ctgtccgcgc catgcctcgg atggggttaa cgacgacagg gtatccgacc aattcgagga
      541 tttcgatgtc tccgggactg tcgccgtagg caaaagaact ggccagttcg atgtcctttt
      601 ccttcgcatg cgacaccaca agttctcgtt ttcccttccc atacggaagc ggtggaatga
      661 tctcccctgt gtagatatcc tctcgacgtt ccagccgggc tgcaaacagg gtcggcacgt
      721 tcaacgcttc ggccagaggg gtgatgagga aatcgggggc accagagacg agaatcagtt
      781 gatggccgga ttggcgatgg gcttcgagac gttcacgccc tttcgtcgac aagagtccga
      841 gcatctcatg atgaaaaaat tcgcgtgcac acgtctcgat gaccgccgga tttttccctt
      901 cgagatatac ctttcgctca cgcaggggat gaatggatag gtccgaagcg tgtcggatca
      961 gccaggttac actgcgtcgc agttcaagcc atccaacaaa gcccctacgc cagagatagc
     1021 gaaagaatcg cacctcacag gcctctcctg gaatgagtgt gttgtcgaca tcgaacaaag
     1081 cggcaagcga tgctgaggat gcagacgtgg ccgcccggac aagagaagga tggagatcgt
     1141 gatagccttg catgctgttg tatcaattga ccgatcgaca gaaggtcaat cttactgaac
     1201 aggccgttga ttgacaagga ttttggccta cttctataat gccgctccct cttgtgaccg
     1261 agcatcgaac tctctcgggg cacgcccaaa atgcccttga acgaggccag agggagcctg
     1321 cgaacgaggc gtattcttta tcgggacggt tgagggggat aggcgactga gaacgtcgct
     1381 ggaagacatt ttcagcaatc taacatgccg aagtggtgga atggcagaca cgcacgtttg
     1441 aggggcgtgt ggcgcaagcc gtgcgggttc aagtcccgcc ttcggcacca actcatataa
     1501 gtgaaaccgt gatgacgtct gctagtgcta cttcaccgcg tcgccgaacg agtcaatcgg
     1561 cgaaatcacc ttcgatccgc ctactcttag ttgacgacca cgaagtggtt cgagtgggac
     1621 ttcgaaccgt gttgcacaac aaccaaggca tcacaatcgt cggggaagcg gataataagg
     1681 cacaagcggc gaaggaagcg aagcgcctca gaccggatgt gatcttgatg gacgttcggc
     1741 tcccggatgg gactggcgtc gaggcctgcc gggagatatt gtcggacaat cctgactcga
     1801 gggtgatctt cctgacatcc tacaccgacg acgattccgt ccttgcggcg gtcttggccg
     1861 gtgcctacgg ctacgtcctc aaagaaatcg attccgccgc gctgattcgg gccattcgaa
     1921 cggtctccac cggtcgatcg attctggacc cgaatgtcac gaagcgcgct ctcgaatgga
     1981 tcaagaccgt cccgacggcc gacgggcctt cccgcgcctc gtcactttcc ccacaggaag
     2041 agcgagtcct ggcgctcgtt gccgaaggac tgaccaacaa ggaaatcgcc ttatcgctca
     2101 agttgagtga taagaccgtg aagaactacc tggccaacat gtttcagaaa ctgcacatct
     2161 ctcggcgcgc
//
LOCUS       DATDNW010000152         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_837699, whole genome shotgun sequence.
ACCESSION   DATDNW010000152 DATDNW010000000
VERSION     DATDNW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837699"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VI359_03735"
     CDS             <1..249
                     /locus_tag="VI359_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973397.1"
                     /translation="GDEAVPFWQSLLTEWSWTNRKKKEDLALLSAESLGKLATPAAIA
                     ALELGQKKAGSAVRQACVAALAQAEKQRNVKQTATLQK"
     gene            261..1829
                     /locus_tag="VI359_03740"
     CDS             261..1829
                     /locus_tag="VI359_03740"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEY6973398.1"
                     /translation="MSTSPTNPTTPQTEKTDEAVQPILTHEKSLYNKIAQGSEAGDIL
                     DQQMAMLGIQLITQLNVLLKTSRIHGRTNAALDKPVDAMLTLIKTLAHDQPITLRLQN
                     DFLFLGNQHLKINAQQMAIATSIIDALQAWKIGGVSFASSTASKDLREFAYLFVSLDP
                     ATKTLDDFRTELSTREVAGITLEEPRELIFRRATSSDTASAPDERLKRKVSAKQGYTS
                     AIQSVGNLMQSTRDGGSVSFKQAKRAIQNIVDLMMQDQSTLLGLTNLRCHDQYTHNHS
                     VNVALLSMALANRAGYPKVHLADLGLAALFHDVGKCAISLDVLNKPGEFTKEEWDLMR
                     THPTEGVLTLIRLRGLGNVPARMAAASFEHHMNYDFSGYPKLALPWTQSLTSRIVTIA
                     DCYDAMTSSRVYRREPMSPANVLKIMFAKSGQSFDPILIKLFVNCVGFIPIGSLVMLD
                     TEELAVVLKPAEEKSNAERPLVKVIADQQGAPIDDGSEFDLTEKDDSGTYRRSIIRLI
                     DNTEHKFDTGRYFV"
     gene            1929..2489
                     /locus_tag="VI359_03745"
     CDS             1929..2489
                     /locus_tag="VI359_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY6973399.1"
                     /translation="MRTTGALLGRMFLLLALVGTVTMGSFSFTHAAEAFKMGVVDPQA
                     VLEKSKAGRRALDGLKDYVSTRQKLLSRDEEDLRNTEKQLKEQIAKLSESEKKDKETQ
                     FRGKIQEYQKRAQEFNQELQAKQKELVDDYMKRIASATQTVAEKGGFSIVVDKGSEQT
                     VKIVIYSKDTIDLTDQVIKEFDRVNK"
     gene            complement(2535..2903)
                     /locus_tag="VI359_03750"
     CDS             complement(2535..2903)
                     /locus_tag="VI359_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY6973400.1"
                     /translation="MKHNPGFLKLVDEVRKRVNECTVADAKARLDRGEPLYFIDVRED
                     HEFAKDHASGARHLGKGVLERDIETVVPDKRASIILYCGGGYRSALAADALRQMGYTN
                     VVSMDGGIKAWRDAGYPLES"
     gene            complement(2923..3936)
                     /locus_tag="VI359_03755"
     CDS             complement(2923..3936)
                     /locus_tag="VI359_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY6973401.1"
                     /translation="MQPVNNPPNPFESQHRELLEPAPRVQLQMYEDSTRSILSRNDSP
                     DLPFRWSLNPYRGCFHACAYCYARPTHEYWGFGAGTDFDSKIIIKRDAPALLRQVFDK
                     PSWKGELIVLSGNTDCYQPLEASLELTRGCLEICLEYRNPVAIITKAALVMRDLDLLQ
                     RLHRDAWVRVYFSIPFADDAVARVVEPHAPSSRKRFEAMAALSEAGLSTGISVSPIIP
                     GLNDQDIPDLLTRARAAGAVEAMATLIRLSGPVEPVFLERMSAAFPDRIAKITHRIRE
                     VRGGQLSNGEFFERHHGTGSYWAMIERLVEVTKRKVGFPMDRETAIPQSFRRPGLEQT
                     VLF"
     gene            4077..>4679
                     /locus_tag="VI359_03760"
     CDS             4077..>4679
                     /locus_tag="VI359_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY6973402.1"
                     /translation="MYDLRALRDNLDAIRDQLGPRGADVPWDNLRKLVEERRALTTQV
                     EHLRHELKHGSDQVAKLKRDKQPADDAMAAMKTVGERIKNLEAGLREVEDTLIDLNFR
                     IPNLPHSSVPVGRDPSDNVEVRSHGNVPQLKTPPRPHWDIGEALGILDFDRAAKIAGA
                     RFAVLTGAGARLERALINYMLDLHTTRHGYREVLPPFMVNR"
BASE COUNT         1151 a         1367 c         1185 g          976 t
ORIGIN      
        1 ggcgatgaag ccgtcccctt ctggcagagc ctcctgacgg agtggtcctg gacaaaccgc
       61 aagaaaaaag aagatctcgc actcctgtcc gctgagtcac tcggtaaact ggctacgccc
      121 gcagccattg ctgcgctgga acttggccag aaaaaggcgg ggtcggccgt acgacaggcc
      181 tgtgtggctg cgttggcaca agccgagaaa caacgaaacg tgaaacagac tgccaccctt
      241 caaaaataac aagtgatctg atgagtacat cgcccacaaa tccaaccact ccacaaaccg
      301 agaagacaga tgaggcagtc cagccgattc tgacgcatga aaagtctctc tataacaaaa
      361 tcgcgcaagg atccgaggcg ggagacattc tggaccagca gatggccatg ctgggtatcc
      421 agcttatcac ccagctcaac gtcctgctga aaacctctcg catccatggc cggaccaatg
      481 cggcgctcga caagcccgtg gatgcgatgc tgactctgat caagacgctt gcccacgacc
      541 aaccgatcac gttgaggctt caaaacgatt ttctcttcct gggcaaccag catctgaaga
      601 tcaacgctca gcaaatggcg atcgccacga gcatcatcga tgcgttacag gcatggaaaa
      661 tcggcggcgt ttcctttgcc tcttcgaccg cctcgaaaga tcttcgcgag tttgcctacc
      721 tgttcgtcag tcttgatccg gccacgaaaa cgctggacga cttccgtacc gaactgagta
      781 cgcgggaggt ggcaggcatt acgctggaag aaccacgaga gttgattttc cgtcgcgcaa
      841 cctcgtctga taccgcctct gcgcctgatg agaggcttaa acggaaggtc agcgcaaagc
      901 agggctatac ctcagcgatt cagtccgtgg gaaatctcat gcaatcgact cgcgatggag
      961 gatcggtgag cttcaagcag gcgaagcggg cgatccagaa catcgtcgac ttgatgatgc
     1021 aggaccagtc taccttgctt gggctcacaa atttacgctg ccacgatcaa tatacgcaca
     1081 accattcggt caacgtcgca cttctctcca tggcactcgc aaatcgtgcc ggctatccca
     1141 aagtccacct tgctgatctg ggattagccg ctctcttcca cgatgtcggg aaatgcgcga
     1201 tctcgctaga cgtcttgaat aaaccgggcg agtttacaaa agaagaatgg gacctcatgc
     1261 ggactcatcc gacagaaggc gtgctgacgc tcattcgatt gcggggcctc ggcaatgtcc
     1321 cagcacgcat ggcagccgcc tcgtttgagc atcatatgaa ctacgatttc tccggctacc
     1381 ccaagctggc tcttccatgg acgcaaagcc tgactagccg gatcgtcacg attgccgact
     1441 gttatgatgc gatgacgtct tcccgcgtct accgccgaga gccgatgtca ccggcgaacg
     1501 tcctgaagat aatgttcgcc aaaagcggcc aatcctttga tccgatactg ataaaactct
     1561 tcgttaattg tgtcggcttc attcccatcg ggagcctagt catgctggac acagaggaac
     1621 tggccgtggt cttgaagccc gccgaagaga aatctaacgc tgaaaggcct ctcgtcaaag
     1681 tcatcgccga tcaacaggga gcccccatcg acgatggatc cgaattcgac ctcacggaaa
     1741 aagacgactc cggcacctac cgacgtagca tcatccgcct gatcgacaat accgaacaca
     1801 agttcgatac cggccggtat ttcgtttagt gcaccaccat ttcttcatct cttgacaggg
     1861 ctcagttccc cagttattct cgcccagttt ggcgaccctc gctgtttact ctaaaaggag
     1921 cacatgttat gcgcacaacc ggggccctat tgggccggat gtttttattg ttggcattgg
     1981 tcggcaccgt caccatgggg tctttctcgt tcacgcacgc ggctgaggct ttcaaaatgg
     2041 gcgtggtcga tccgcaggct gtgttggaaa aatcgaaggc cggcagaaga gcacttgatg
     2101 gattaaaaga ttacgtttca accaggcaga aactgctatc gcgcgatgag gaggacttgc
     2161 gcaacaccga gaaacagctc aaggagcaaa tcgcaaagct tagcgaatcc gaaaaaaagg
     2221 acaaggaaac acagttccgc ggaaaaattc aagagtatca aaaacgcgca caagaattta
     2281 accaggaact tcaagccaaa cagaaggagc tggttgacga ctatatgaag agaatcgcgt
     2341 cggccaccca gaccgtcgcg gaaaagggcg gattttccat cgtcgttgac aagggcagtg
     2401 aacagaccgt caagattgtt atctacagca aagataccat tgatctcacc gatcaagtca
     2461 tcaaagagtt cgaccgagtc aacaagtagc ttgcccacga tctttcactg ggatctgctc
     2521 ccacagatcc cagttcagct ttccaaaggg tatccggcat ctcgccaggc cttgattcca
     2581 ccatccatag aaaccacatt cgtataaccc atctgccgga gagcatctgc tgccagagcc
     2641 gaccgatatc cacctccaca ataaagaatg atcgaggccc gtttgtcggg cacaacagtc
     2701 tcaatgtcac gttcgagaac gcctttgcca aggtgtcgcg caccacttgc gtgatccttg
     2761 gcgaattcat ggtcttctcg tacatcaata aaatagagcg gctccccgcg atcgagcctg
     2821 gctttcgcgt ccgcgaccgt gcattcattt acccgttttc gtacctcatc caccaacttg
     2881 agaaaccccg gattgtgttt catcggtgat cggtcccctc actcagaaca ataccgtctg
     2941 ctccagccca ggtcgccgaa aactctgggg aatggcagtt tctcgatcca tcggaaatcc
     3001 caccttgcgt ttggttacct caaccaaccg ctcgatcatc gcccaatagg acccggttcc
     3061 gtggtgccgt tcgaaaaact ctccgttgct tagttgccct ccccgcacct cccgaatccg
     3121 atgcgtaatc ttcgcaatcc ggtcaggaaa ggcggcgctc atgcgctcaa gaaataccgg
     3181 ttccacaggg ccggacaaac ggatcaatgt agccatcgcc tccacagctc ccgccgctcg
     3241 agcgcgcgta agcaagtctg ggatatcctg atcgttcaga ccgggaatga tcggtgagac
     3301 cgagattccc gttgacagtc ctgcctccga caatgccgcc atagcctcga accgcttgcg
     3361 gcttgatgga gcatgaggct ccacaactct cgccactgcg tcatcggcga aaggaatgct
     3421 gaaatagacc cgcacccatg cgtcacggtg caaccgctgc aacaaatcca ggtcacgcat
     3481 gaccaaggcc gctttcgtga tgatcgcgac cggatttcgg tactccagac agatttcaag
     3541 gcagccccga gtcaattcta acgacgcctc cagcggctga tagcaatctg tgttgccgga
     3601 cagcacgatc aactcgccct tccaggacgg cttgtcgaac acttggcgaa gcaacgctgg
     3661 cgcgtcgcgc ttgatgatga tcttcgaatc aaaatcggtt ccggcgccaa acccccaata
     3721 ctcgtgggtc ggtctggcat aacaataggc gcacgcgtgg aagcatccgc ggtaggggtt
     3781 cagactccat ctgaacggca aatcagggct gtcatttcgg ctgagaatgc ttcgggtcga
     3841 atcctcgtac atctgtagtt gaacgcgggg agccggctcg agcagttcac ggtgctgtga
     3901 ctcaaacgga ttgggtgggt tgttcactgg ctgcatggtg tatggtctgc aatctttctc
     3961 cacaagtcaa tgggcggatc ttcatgagat tgccgttttg gcacctacac gccgattgat
     4021 tgactaaccc ccacctcgat gttagattcc agccgcttta gcggagatct catcaaatgt
     4081 acgaccttcg cgcgctacgc gacaacttgg atgccattcg tgaccaactg ggcccccgcg
     4141 gcgccgacgt gccctgggac aatcttcgta aactcgtcga agagcgacgc gccctgacca
     4201 cgcaggtcga acaccttcgt catgaactca agcacggatc cgaccaggtc gccaagctca
     4261 aacgagataa acagccagca gatgacgcga tggctgcgat gaaaaccgtc ggcgagcgaa
     4321 tcaagaatct tgaagcggga ctacgggaag tcgaggatac actcatcgat ctcaattttc
     4381 gtatccccaa tcttccgcac tcctccgtac cggtcgggag agacccatcg gacaacgtag
     4441 aagttcgctc ccatgggaac gtaccgcagc tgaaaacccc tcccaggccc cactgggata
     4501 ttggcgaagc gctcggcatt ctggattttg atcgggcggc gaagatcgcc ggagcacgat
     4561 ttgccgtgct gacaggagca ggggctcgac tcgaacgggc gttgatcaat tatatgcttg
     4621 accttcacac cactcgacat ggatatcggg aagtcctccc gccctttatg gtcaaccga
//
LOCUS       DATDNW010000153         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_156876, whole genome shotgun sequence.
ACCESSION   DATDNW010000153 DATDNW010000000
VERSION     DATDNW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156876"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VI359_03765"
     CDS             complement(<1..399)
                     /locus_tag="VI359_03765"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY6973403.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKIMVAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIAQEVEGPTICSLARARPEDVDRAWDALKGAPKVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQRARGYV"
     misc_binding    complement(619..855)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(852..1655)
                     /gene="pssA"
                     /locus_tag="VI359_03770"
     CDS             complement(852..1655)
                     /gene="pssA"
                     /locus_tag="VI359_03770"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY6973404.1"
                     /translation="MKTAGLRQAMGRAAKQRHAMHLIPNLFTTGNLFCGVYAILSVFN
                     ANYMAAAIAILVAMVFDVLDGKSARLTNSTSHFGLEYDSLSDVVSFGVAPGLLIYSWA
                     LSGQGTFGIAVMFAYVAMGAVRLARFNATVALSDSKYFTGLAIPAAAGVVASLVIFDH
                     YIVRMGAEIKPILVLFITLSLSFLMVSTIKYRSFKDMKFRGGRHFTYLVWGILALMLV
                     AAWPQVMLFVTFAGYALLGPVERLVGLVAKVIGKRPTARAEHSIIEPKF"
     gene            complement(1652..>2256)
                     /locus_tag="VI359_03775"
     CDS             complement(1652..>2256)
                     /locus_tag="VI359_03775"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEY6973405.1"
                     /translation="FVGIGAGVTLLAGFLGWTVVAVLAGGLIVFTAWFFRNPTRVIPQ
                     GPGLVVAPGDGTVIAIEEEFEPRFLKDQSVRLTIFLNVLDVHVNRIPCEGVVEDIQYQ
                     PGAFLVASKPEATLKNEQNALFLRTAQGARVLCVQVAGLIARRIVCWLSPGDRVARGE
                     RFGLIRFGSRMDTFLPLGTALKVAVGDRVKGGETIVGVLR"
BASE COUNT          543 a          717 c          551 g          445 t
ORIGIN      
        1 gacatagcct ctcgcccgct gcaccatctc aaccgcgcgt ttcttggctt cctcccgcgt
       61 catacgaaac tggtgtttca aatggatgtc cgacgtggaa aggaaggtgt gaatgcgaac
      121 tttgggcgcc cctttgagcg catcccacgc tcggtcaaca tcctcgggac gggcccgtgc
      181 caagctgcag atcgttggtc cttccacctc ctgtgcgatc cgccgcacag cctcgaagtc
      241 accaggagaa ctataggcaa acccggcttc gataatgtcg acgccgagac gggccagttg
      301 cttcgccacc ataattttct cctccacatt catgctagcg ccgggagatt gctctccgtc
      361 ccgcaacgtc gtatcgaaaa ttcgtatcat gcgtgtcatg tgtgcacccc tctcctcatc
      421 aggctgctca aaaagtccga cattctcacc cgcccgcccc tggcgcgccg agacgcgccc
      481 tttcccaggc aaggctgcag gcgcaggaaa tacccccggc ataccctctg ggatatgttg
      541 atgatgcttt caagcccaga acgaagctgg tggccgtttt gagcagtctg caaaaacaca
      601 aaagcctcca tcccagaaga cagggacgaa ggcttcacgc actccgtggt accaccctgg
      661 ttcggcgatg gacggccact ctcgccgttc atcacccttc attcggcccg tcacgagggc
      721 aagacggaat ccgctactct cgattcacgg attcggctcg gaggcgagtt ccgcgctcaa
      781 cgggctggtt cgcaccatcc accagctctc tcgattccgt ccgaggcgcg tactattccc
      841 cgtcacagcc actaaaactt cggctcgata atcgaatgct cggctcttgc cgtcggacgc
      901 ttgccgatta ctttggccac gagcccgacg agccgctcca ccggaccgag taaggcatat
      961 ccggcaaagg ttacaaataa cataacttga ggccacgccg ctactagcat aagggcgaga
     1021 atcccccaca ccagataggt aaagtggcga ccgcctcgaa acttcatatc cttgaagctg
     1081 cgatacttga tggtgctgac catcaagaac gacaaagaca gggtaatgaa caacacgaga
     1141 atcggcttga tttccgcccc catccgtaca atgtaatgat cgaagatcac gagcgatgcc
     1201 acaacgcccg ctgcagcagg gatggccaat ccggtaaagt atttgctatc ggataaggca
     1261 acggttgcgt taaaccgagc cagtcgcact gcccccatgg caacataggc gaacatcacc
     1321 gcaattccaa aggtcccctg accgctcagc gcccaggaat agatcagcag gcccggcgcc
     1381 acgccaaacg acaccacatc ggacaaggaa tcatattcaa gtccaaaatg actcgtgctg
     1441 ttggtcagcc gggcagactt tccatccaac acgtcgaaca ccatcgcgac cagaattgcg
     1501 atcgcggccg ccatgtagtt ggcattgaac acggacaaga tcgcatagac tccacagaac
     1561 aaattgcccg tcgtgaacag attcgggatc agatgcatgg catggcgttg ttttgcagct
     1621 cgtcccattg cttgacgtaa cccggcagtt ttcatcgcag cacccccaca atcgtttcgc
     1681 caccttttac ccgatcaccg acggcgacct tcaaggctgt cccgagcgga agaaaggtgt
     1741 ccatgcgtga cccgaatcga atgagtccga atcgttcacc gcgcgccaca cggtcacccg
     1801 gcgagagcca gcaaacgata cgcctcgcaa tgagccccgc aacttggaca cagaggactc
     1861 tcgcaccctg cgcagttcgg agaaacagag cattttgttc gttcttcaac gtcgcctccg
     1921 gtttgctcgc caccaaaaat gctcctggct gatactgaat gtcttcaacc actccttcac
     1981 acggaatgcg attcacatgc acatcgagga cgttcaaaaa gatggtgagg cgaacgcttt
     2041 gatccttaag aaatcggggt tcaaactctt cctcgatggc gatcactgtg ccatcgcctg
     2101 gcgcgacaac cagtccaggt ccttgaggaa tgactctcgt tggatttctg aaaaaccagg
     2161 ccgtgaagac tataaggcct ccggccagca ctgcaacgac cgtccacccc aaaaaccctg
     2221 caagtagtgt aacgccagcc ccgatcccga cgaagg
//
LOCUS       DATDNW010000154         3323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_494839, whole genome shotgun sequence.
ACCESSION   DATDNW010000154 DATDNW010000000
VERSION     DATDNW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494839"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1316)
                     /locus_tag="VI359_03780"
     CDS             complement(69..1316)
                     /locus_tag="VI359_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY6973406.1"
                     /translation="MIVSQSRRVRVALGAAIVLSLLAGGHLVSTGLFAQETAKSQATV
                     EKAFPNSSKCKRCHERVFEEWETSPLSRSIHSPAFRTALDEYVQFSGGKDNALCFRCH
                     APHVREFPDHAKLFLDQVKSGEPAIDGVACAQCHLIKQVDRAKHPPEPKYDLGSKVLY
                     GPYKDFVQNLAHQSMDLDLFRKSDLCLNCHQSVPSASNLGKTNDLLGNWDQSKSVKSG
                     KECQSCHMPEQIGESANGESKRKVANHTFPGRIGKLRQEAAKLDVQTKIEGDKTTVTV
                     KVQSLVPHNLPTTHPAWASVMLDLDIKGKNLKTVFTDKRVYGRVYQDAKGQKTNFDFE
                     AVKVLEDTVLKPEETRVETFTFPTPKDTKSFDVEITLNYAAISGPAAFLQRVEAESPK
                     GSQDPVFQPIPIVKYTENIPINK"
     gene            complement(1339..2634)
                     /locus_tag="VI359_03785"
     CDS             complement(1339..2634)
                     /locus_tag="VI359_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY6973407.1"
                     /translation="MRIGQRLAVGLFLAIVVGVNGVYHGFAIAQKADGQAPADWVAEI
                     EKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKTFGRVDAALLHGTSLESPVFRTVLGV
                     WVQTNPTTEERRRCLSCHVPSTTVFPQHAEKIVGQILAGKPKVEGIGCASCHLIKQVE
                     STPGPPPTFKIEPGKMIYGPYKDPEENLVHPATQSDLFRGANYCASCHFDKVKDVTQK
                     DIPGEILQGTICQDCHMEPSTGSSTSKRGAMTRAIGRHWFRGVVIPGILLKNRNLQAE
                     WMPRVDIDTTKSASAVEGTALVKVGSLPHSFPDGDPVLKQFFLTITVKDAQGKVLGQD
                     TKQFGLPYDKILRGPIADPFIKGGNTRKVPFSQAIPAGATPATVEAVLSYALIPTPDT
                     ALRDKYLATLANDKERDAAKKILDEYTQPRVLTYRAKTL"
     gene            complement(2644..3060)
                     /locus_tag="VI359_03790"
     CDS             complement(2644..3060)
                     /locus_tag="VI359_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973408.1"
                     /translation="MCVGLSLMMVGGGVPAGVRAADQEPMTVEEATRLGEEFGVIVGE
                     VDEEIQKELKLQRPQGVAVFEVIGNSRADYAGIKVRSVIKEIDKKEIRNMIDFGRSIK
                     VAMKECNFTLGTYEPADPGDPVGWGVNFHFVGCKRD"
BASE COUNT          659 a          987 c          889 g          788 t
ORIGIN      
        1 tcgtacccaa tgcggcggta gaagcgtccg acctctcacc tctccgcttc acaagagacg
       61 aacgacgttt acttattgat tggaatgttc tcggtatact tgacgatggg gattggttgg
      121 aagaccgggt cctgagaccc cttgggtgat tcggcttcga cccgctggag aaaggctgca
      181 ggtccgctaa tggcggcata gttgagcgtg atctcgacgt cgaagctctt cgtgtctttt
      241 ggcgttggga aggtgaaggt ctcgacgcgc gtttcttccg gcttcaagac ggtatcttcc
      301 agcaccttga ccgcttcgaa atcgaagttt gttttctgcc ccttggcatc ctgatagacc
      361 cgtccataga cgcgcttgtc ggtaaagacg gtcttgagat tcttgccctt gatatcgagg
      421 tctagcatca cactggccca ggccggatgg gtcgtgggca gattgtgcgg caccaggctc
      481 tggactttga cggtcactgt cgtcttgtcg ccctcgatct ttgtttggac gtccagcttg
      541 gctgcttcct gacgcagctt cccgatgcga ccggggaagg tatggttcgc gactttacgc
      601 ttactttcgc cgttcgccga ctccccgatc tgctccggca tgtggcacga ctggcattcc
      661 ttgccggact tcaccgactt actttggtcc caattgccca acagatcgtt ggtctttccc
      721 agattcgacg cactcggcac cgattgatgg caattcagac agagatccga tttgcggaac
      781 agatccagat ccatcgattg atgcgccagg ttctggacga aatccttgta cggcccgtag
      841 agcaccttgc tcccaaggtc gtatttcggc tccggcggat gcttggctcg atcgacctgt
      901 ttgatcaaat gacattgcgc gcaagccacg ccgtcgatcg caggctcgcc ggatttgact
      961 tgatcgagaa aaagcttggc atgatcggga aattcacgga catgcggggc atgacagcga
     1021 aagcagagcg cgttgtcctt cccgccggaa aactggacgt attcatcgag ggccgtgcgg
     1081 aaggccggcg aatgaatgga gcgggagagg ggcgacgttt cccactcctc gaacacccgc
     1141 tcgtggcacc gcttgcactt gcttgagttg gggaacgcct tttcgacggt cgcctgagac
     1201 ttggctgttt cttgcgcaaa aagaccggtc gaaaccagat gcccaccagc gagcagagac
     1261 agcacgatgg ccgctcccag cgcaactctc acacgtcgag attgactgac aatcacgtcg
     1321 tttctcctta cgttgccact acaaggtttt ggctcgatag gtcagcacac gcggctgggt
     1381 atactcgtcg agaattttct ttgccgcatc ccgttctttg tcgtttgcta gcgtcgccag
     1441 atacttgtct cgcaacgcgg tatccggcgt tgggatcaag gcatagctca gcacggcttc
     1501 gaccgtcgcc ggcgtggccc cagcggggat ggcttgtgag aacgggactt tccttgtatt
     1561 cccgcctttg atgaacggat cggcaatcgg cccgcgcagg attttgtcgt acggcagtcc
     1621 aaactgcttc gtgtcctggc ccaagacctt gccttgggca tccttgaccg tgatcgtgag
     1681 gaaaaattgc ttcaagaccg gatcaccgtc cggaaaacta tggggcaggc ttcccacttt
     1741 gaccagggcc gtgccttcga cagccgaggc ggacttggtc gtatcaatgt ccacgcgcgg
     1801 catccactcc gcctgaagat tcctgttctt gagcagaatg ccgggaatca cgacgccgcg
     1861 gaaccagtgc cggccgatcg cacgcgtcat cgcaccgcgt ttggaagtcg aactgccggt
     1921 ggagggctcc atgtggcaat cctggcagat cgtgccttgc agaatttctc ccggaatatc
     1981 cttctgcgtc acgtccttga ccttgtcgaa atgacaggag gcgcagtagt tcgctccgcg
     2041 gaagagatcg gattgcgtgg ccggatggac gagattctcc tccggatctt tgtacggacc
     2101 atagatcatc ttccccggct cgattttgaa tgtcggagga ggacccggcg tcgactcaac
     2161 ttgcttgatc aaatggcagg aggcacagcc gatcccttcg accttcggct tcccagccaa
     2221 tatttggccg acaatttttt cagcatgttg tggaaacacc gtcgtggagg gtacgtggca
     2281 ggacaggcaa cgacgccgct cctcggtggt cggattcgtt tgcacccaga cgcccaggac
     2341 ggtgcggaac accggggatt ccagcgaggt gccgtgtaac agcgcggcat cgacccgacc
     2401 gaacgtcttc aggtcgggag tctgctcgcg catacccttc cattcttcat agtgccggtc
     2461 atgacactgt ttacaatcct cggaccggat gaagattttc tcgatttccg caacccagtc
     2521 ggccggtgct tgaccgtcgg ctttctgcgc gatcgcgaac ccatggtaca caccattgac
     2581 acccaccacg attgcgagaa agagtccgac ggctagacgc tgtccgatgc gcatagattg
     2641 ttcctagtct cgcttgcacc cgacgaagtg aaagttgaca ccccacccga ccggatcgcc
     2701 cgggtctgca ggctcataag ttcccaacgt aaagttacat tctttcatcg ccactttgat
     2761 cgatcgtccg aaatcgatca tatttctgat ttccttttta tcgatctcct taatgactga
     2821 gcgcacctta atccctgcat agtcggcccg tgaattgccg atcacttcga agaccgctac
     2881 gccctgaggc cgctgcagct tcaattcttt ctgaatttct tcatcgactt cgccgacgat
     2941 cacaccgaac tcttcaccca gccgcgtggc ctcttcgacc gtcatgggct cctgatcagc
     3001 cgctcggacg ccagcaggca cacctccgcc gaccatcata agcgacaggc caacacacat
     3061 aaaaaaagcc ctcctgcggc gaagggcttg ctgtcgtcga tccgtcaaac cgatgtctcc
     3121 tccaatgctg acgcacacct tcttgttaca tcacaccaag aactctacag tttcgcaacc
     3181 ggatcgatca cgagctgaaa ccacggcgcc accgcctttt gcccgttccg ttccttgttc
     3241 tctccgttcc agaccgcgaa cgagaccgtt tgtattcgac cgggaacgag cttggcttca
     3301 ttttcctgct cttcgcttga gag
//
LOCUS       DATDNW010000155         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_494918, whole genome shotgun sequence.
ACCESSION   DATDNW010000155 DATDNW010000000
VERSION     DATDNW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494918"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2296)
                     /locus_tag="VI359_03795"
     CDS             complement(<1..>2296)
                     /locus_tag="VI359_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY6973409.1"
                     /translation="FLRTSPAILGDIEQIPVRSKVVAVGAVLADHSIEAGIAWLSESP
                     RLLRMLPSPEWQMKMLQYGALLGEQDPEATLAYLRRCPELIGLIGDSTDAVSRFEKWF
                     KAGMEVLAYSIEGARAYFALESQTALDSVEKALSGVPLRQVARTIKLFVQGLCGTEFA
                     IQALPASLAPDAPVRATVSPDGRTISLPSLLRRYPTTEDNMRLYLVMAAHEAGHVEFG
                     TYRLRLEPLASLVAEVRERYGRTDRTDPETLTELFHLYPQPRLAQDIWTLLEDARVEY
                     LLQQQYPGLRRDLQRLAREAVETKSLTHGLTIKELIVDQLLQLSSADPESVPIHEAIK
                     EEITILWPMCRSVRVTTATAEDVVRVTHDIYVRLEELLAPKSEMIRADQVDALSQDLG
                     VGPSASEESSEDYRPVTNFVYRGAMNPEFIRRRDAEEQTKREQPRSDEINRLASTAAG
                     AQKDATSGQQARKDTQTSSEIDVLSGGRQLPPHVEELLALDVDQPTSVDRTGAGDRAI
                     RYPEWDQEIHDYRLNWCRVVERAADERPDDVVAATLQAQGGIVSMLRRFFESLRPPGL
                     KRVAGQVDGDEIDLDAVVRASAERASGAEVSDRMYIRRDRKERDVAAAFLVDVSGSTS
                     RQLDGGRRVIDVEREGLVLLCEALEAVGDQYALYGYSGQGRGHVDFLVIKDFADRLGG
                     QAAHRLAGLMPLQQNRDGAAIRHATTKLLAREAKTNLLIVVSDGRPLDDGYKDEYSLE
                     DTKTALREAKRRGIHPFCITIDRDA"
BASE COUNT          486 a          729 c          624 g          457 t
ORIGIN      
        1 cggcgtcgcg atcgatcgtg atgcaaaagg gatggatccc tcgccgcttc gcttcgcgca
       61 aggcggtctt cgtgtcctca agtgaatact catctttata gccgtcatcc aggggccggc
      121 cgtcactgac gacgatcaag agattggtct tggcttcacg agccagcagc ttcgtcgtcg
      181 catggcggat tgccgccccg tcacgatttt gctgcagagg catcagcccg gccaatcggt
      241 gcgccgcctg tccgcccaac cgatcggcaa aatccttgat caccaagaaa tcaacatggc
      301 cgcggccctg gcctgaatac ccatagagcg catactgatc gccaactgct tccagcgcct
      361 cgcacaacaa caccagcccc tctcgctcga catcgatgac acgacggccc ccgtcaagtt
      421 ggcgactagt ggaaccgctt acgtccacaa gaaacgccgc cgcaacgtct cgctccttgc
      481 gatcccgccg gatatacatc cgatcggaca cctcagcccc tgaggctcgt tcggccgacg
      541 cgcgcaccac ggcgtcgaga tcgatctcat ccccatccac ttgaccagcc acacgcttca
      601 accccggtgg acgcaaactc tcaaagaacc gccgcagcat ggacacaatg ccgccttgtg
      661 cctgcaacgt ggccgcgacg acatcgtctg gacgttcatc cgcagcgcgt tcgacgacac
      721 ggcaccagtt gaggcggtag tcgtgaattt cctgatccca ttcgggatag cgaatcgctc
      781 gatctccggc gccggttcgg tccacggatg tcggctgatc gacgtccaag gcgagtaact
      841 cctccacgtg agggggcagt tgccgtccac cactcagcac gtcaatttct gaagaagtct
      901 gagtgtcttt ccgtgcctgt tgaccagatg ttgcgtcctt ttgcgctcca gccgcagtgc
      961 tggccagtcg attgatctca tccgatcgcg gctgctcccg tttcgtctgt tcctccgcat
     1021 cacgccgacg aataaactcg gggttcatcg cgccccgata cacgaaattc gtcacgggac
     1081 gatagtcttc actgctctct tccgaggccg agggccccac acccagatcc tgtgagagcg
     1141 cgtcgacctg gtcggcacgt atcatttctg acttcggcgc caacaactct tcgagtcgga
     1201 catagatgtc atgcgttaca cgaacgacgt cttccgccgt cgccgttgtg acccggacgg
     1261 accggcacat cggccagagg atcgtgatct cctccttaat tgcttcatgg atgggtacag
     1321 attctggatc ggcgctggaa agctggagga gttgatccac gatcaattcc ttgatggtca
     1381 ggccatgagt caacgacttc gtctcgaccg cttcacgagc caacctctgc aaatcgcggc
     1441 gaagaccggg atactgttgt tgaagcagat attccacgcg cgcatcttcg agtaacgtcc
     1501 agatgtcctg tgcaagccgt ggttgcggat agagatggaa cagttcggtc aaagtttcgg
     1561 gatcagtccg atcggtccgg ccatagcgtt cacgcacctc agcgaccaga ctggcaagcg
     1621 gctcgagcct caggcgatag gtgccgaatt cgacatggcc ggcctcatga gccgccatca
     1681 ccagatagag ccgcatatta tcttccgtgg tgggatagcg gcgcagaaga gagggaagag
     1741 aaatagtccg tccatctgga ctgaccgtcg cacgtacagg tgcgtctggc gcgagcgagg
     1801 cgggaagcgc ctgaatcgcg aattcggtcc cacacaagcc ctgcacaaac aatttgatcg
     1861 tgcgcgcgac ctgtcgcaac ggcacgccgc tcaacgcctt ctcgaccgaa tcaagagcgg
     1921 tctgcgactc caaggcgaag taggccctgg ctccttcaat actgtaggcc aggacttcca
     1981 tgccagcctt aaaccacttt tcaaatcggc tgaccgcatc ggtcgaatcg ccgatcagtc
     2041 cgatcagctc agggcagcga cggagatagg caagggtggc ttcaggatct tgttcaccca
     2101 gcaacgcgcc atactgcaac atcttcattt gccattccgg tgacgggagc atacgcaaga
     2161 gacggggtga ctcggacaac catgcgatgc cggcctcgat cgaatggtca gccagcacgg
     2221 cgcccaccgc gacaaccttc gatcggaccg gtatctgttc gatatcgccc agaatagccg
     2281 ggctggtccg gagaaa
//
LOCUS       DATDNW010000156         4506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1177205, whole genome shotgun sequence.
ACCESSION   DATDNW010000156 DATDNW010000000
VERSION     DATDNW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177205"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VI359_03800"
     CDS             <1..262
                     /locus_tag="VI359_03800"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEY6973410.1"
                     /translation="ELFRTVIRFLQTEILDLEPYLAVGIIACTRRILTASAELSHLPA
                     MTETQFYQYLMDVGLNVTVIMVLVVGVFLVRKRPASSPATSP"
     gene            429..815
                     /locus_tag="VI359_03805"
     CDS             429..815
                     /locus_tag="VI359_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973411.1"
                     /translation="MANLLEYVGCVHIYLGPYRGNPIALYLKRTETGCQIGPKAYPWN
                     DVIGTGETPNKAAADFEEKWKTRGLSADMYSGPAWEGGIKPEKPAPPKPPAPSKPAAA
                     TQPSASPEAQPAAASTPAVPSTGEST"
     gene            complement(834..2276)
                     /locus_tag="VI359_03810"
     CDS             complement(834..2276)
                     /locus_tag="VI359_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6973412.1"
                     /translation="MISRLFRTKSIPQILADAEHPDHRLRKTLTAWDLTCLGIGAIIG
                     TGIFVLIGTAIVGDSHRPGAGPGIVLSFILSGIACALAALCYAEFSAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNLLKLAGLELPYWATHPPGGS
                     EGGVANIPAAIIVLLVTIVLVIGIKESARATGIIVMIKLAVILFFIAVGAPAVNPENW
                     TPFMPQGFAGVQSAAAVIFFAYIGFDAVSTAAEEARNPQRDLPLGIIGSLAVCTVLYI
                     SVAAVLTGLIPSSKIDIHAPVAEALSLAGFKWGAAIVAIGAVAGITSVLVVMMLGQIR
                     VFFAMSRDRLLGPWLAKVHPRFGTPHRATILTGVAVAVLAALIPIGDAADMTNIGTFF
                     AFVLVCLGVTILRYTKPDQSRPFRLPFMPFVPILGILACLGLMAGLPQLTWIRFVVWT
                     IVGILVYLFYGLKHSRLAEPAPATGSSSVH"
     gene            complement(2407..3147)
                     /locus_tag="VI359_03815"
     CDS             complement(2407..3147)
                     /locus_tag="VI359_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY6973413.1"
                     /translation="MKPIIGVTPDYNAGDRKEWGGREPTYFLRARYIRAIEEMGGIPL
                     ILPLVADRAARTRLLHDVDGLLLTGSGPDLPPALYGERQRYTFRVMSERRSAFELDMV
                     QLARKADVPVLGICGGMQTMNVACGGSLFQDISSQLAKPLQHRQPKPATALSHAITIT
                     PGSLLRRIMRTVTLRVNSSHHQSVKAVAPSLVVSAVAPDGIIEAIESPTHRFFLGIQW
                     HPEFLFDRYRLHRRLFDAFLRAASHRTG"
     gene            complement(3150..3611)
                     /locus_tag="VI359_03820"
     CDS             complement(3150..3611)
                     /locus_tag="VI359_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973414.1"
                     /translation="MKFYSLLSHGAWNRSVALAIVLALVTVTAVGPAWSQDGQPVAPP
                     VASDSEGGSASSAGMQAAAFFSTVLYAPFKLAFAIGGGIVGGLAYAFSGGNEQAAKSI
                     WQTSMYGTYLITPDHLRGDRPVRFLGVADQTDEPAISQPSPAPTPAPEPVR"
     gene            complement(3755..4222)
                     /locus_tag="VI359_03825"
     CDS             complement(3755..4222)
                     /locus_tag="VI359_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973415.1"
                     /translation="MADEASSDTSSYRVTLFFGPEPVEGKPSIQYCVFNVKKRSWKGG
                     IQVAVEVRRDQLDEIARILRYPEFVEKKLSGLSSEDQLSFRNRANECYAQAMCWCKLD
                     LMLQAGITPENQCLAADVLRDELRDAAIKRAAYITSYILDELDLPPGDATLST"
     gene            4303..>4506
                     /locus_tag="VI359_03830"
     CDS             4303..>4506
                     /locus_tag="VI359_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY6973416.1"
                     /translation="MTTAPHQRVLDIQQALCEAGTMDGWLFYDFRGSDPLAYRILLLD
                     PTLHVTRRWYYWVPAQGTPVKIVH"
BASE COUNT         1014 a         1275 c         1311 g          906 t
ORIGIN      
        1 cgaactgttc cgaaccgtga tcagatttct ccagacggaa attctcgatc tcgaacctta
       61 tctggctgtc gggatcattg cctgtacgag acgaattctc accgccagcg cagaactctc
      121 ccatctccca gccatgaccg aaacgcagtt ttatcagtac ctcatggacg taggtttaaa
      181 cgtcaccgtg atcatggtgc tcgtcgtggg cgtcttcttg gttcgcaaac gcccggcctc
      241 atcccccgcc acatcaccgt agtgttttct cagatgagga attctgtctc caattcgttc
      301 atttcccgca cgcgtcgaac gaccgcaaat tcgaggctct gtcaggagag gggagcaggc
      361 aggtggccct cccacggctt gcctgattcc aagcaggtat ggcatcatgc ctcctcttgg
      421 agacgggtat ggcgaacttg ctcgaatatg tcggctgtgt ccacatctac ctcggaccgt
      481 accgcgggaa tccgatcgcc ctgtatttga agcggacaga aaccgggtgc cagattggtc
      541 caaaggccta tccgtggaac gatgtgattg ggacgggtga gactccgaat aaagcggctg
      601 ccgacttcga agagaaatgg aagaccaggg gtctctcagc cgacatgtat tcaggtcctg
      661 cctgggaagg cgggatcaaa ccagagaaac ccgccccgcc gaaaccacct gctccctcga
      721 agcctgccgc ggctactcag ccaagtgcgt cccctgaggc ccagccagct gcagcctcga
      781 cccctgcggt tccatccacc ggcgaatcca cctagtatct ttcgcttttc ccgttagtga
      841 acactcgacg agccggttgc aggcgccggt tcggcaagtc tgctgtgttt gagcccatag
      901 aacagataaa cgaggattcc tacgatcgtc cagacgacga agcgaatcca ggttagctga
      961 ggcagacccg ccataagacc gaggcaggcc agtatcccca agatgggaac gaatggcatg
     1021 aaaggaagac ggaatgggcg tgactgatcc ggtttggtat agcggaggat agtcacgcca
     1081 agacacacga ggacaaaggc aaagaaggtt ccgatgttgg tcatgtccgc cgcgtccccg
     1141 attggaatca gcgcggccaa cactgcgacc gcgacgccgg tgaggatcgt ggctcggtgt
     1201 ggagtcccaa aacggggatg gacttttgcc agccaggggc cgagcagccg gtcgcgcgac
     1261 atcgcaaaaa acacccggat ttgcccgagc atcatcacga ctaacacgct tgtgatgccg
     1321 gcgacggctc cgatggcgac gatagctgcg ccccacttga acccagcgag actcaacgct
     1381 tctgccaccg gtgcgtggat atcgatcttg ctggagggaa tgaggccggt gagcaccgcg
     1441 gccacggaaa tatacaatac ggtgcacacc gccaacgagc cgatgatgcc caacgggagg
     1501 tcgcgttggg gatttcgggc ttcttctgct gccgtggaaa ccgcatcgaa tccgatatag
     1561 gcgaagaaga taacagccgc tgcggactgt acgccggcga aaccctgcgg catgaacgga
     1621 gtccaattct ccgggttgac cgccggagcc ccgacagcga tgaaaaacag gatgactgcc
     1681 agcttgatca tcacaataat accggtggct ctggcgcttt ccttgattcc aatgacgagc
     1741 acgatggtca cgaggagtac gatgatggcg gcggggatat tcgcgactcc tccttcggat
     1801 cctcccggtg gatgcgtcgc ccagtagggc aattcaagac cggcgagctt caataaatta
     1861 ttgaaataac ccgaccatcc gatggcgacc gcgacgcagg cgacaccata ctccagaatc
     1921 agattccagc ccgtgagcca ggcgagaaac tcgccaaggg tcgcgtagga ataggtgtag
     1981 gcactcccgg cgaccggaat catggcggaa aattctgcgt aacagagggc cgcaagcgcg
     2041 caagcaattc ccgagaggat gaacgacagc acaatccctg gtcccgcgcc gggccggtga
     2101 ctgtccccca cgatcgcggt gccgatgagt acgaagatgc ctgtcccgat gatggcgccg
     2161 atcccgagac aggtgaggtc ccaggccgtc agggtttttc tgagtcgatg atccgggtgc
     2221 tccgcatccg cgaggatctg ggggatcgat ttcgtgcgaa agagacggct aatcaccgat
     2281 ccctcggtgc gagaggagat cgcggtcggt cccgcctgat cgaggacgtg gttgcccata
     2341 tttatgaagt atagcaggcg atgttcgaca atccggctgg tcgatcgagc ggtttcccat
     2401 gcccgcttag cctgtccggt gagaggcggc gcgcagaaag gcgtcaaaga gacgtcgatg
     2461 gaggcggtag cgatcgaaga ggaactctgg gtgccattgg atgccgagga aaaagcgatg
     2521 ggtgggtgat tcgatagctt cgatgattcc gtctggtgcg accgcgctca cgactaacga
     2581 cggtgccacg gctttcaccg attggtgatg ggaactattc acccgcagtg tgacggtgcg
     2641 catgattcgg cgcaacaagc ttcctggcgt gattgtgatc gcatgagaca gtgcggtcgc
     2701 tggtttcggt tgccggtgtt ggagcggttt tgcaagttgg gacgaaatgt cttgaaacag
     2761 gctccccccg cacgcgacgt tcatggtttg catcccgccg caaatgccga gcaccggcac
     2821 atctgccttc cgtgctaact gcaccatatc cagttcgaag gccgaccgac gctcgctcat
     2881 gacacggaag gtataccgtt gacgttctcc atagagagca ggaggaagat cgggcccgct
     2941 tcccgtcagc aagagcccat cgacatcgtg gagaaggcgt gttctcgccg ctcggtccgc
     3001 gaccagggga agaatgagcg ggatgcctcc catttcttcg atcgcccgaa tatatcgcgc
     3061 ccgaagaaaa taagttggtt cccgccctcc ccactccttt cgatcaccgg cgttgtagtc
     3121 aggcgtgacg ccgatgatgg gtttcattgt cagcgaacag gttccggtgc cggtgtgggt
     3181 gccggtgatg gttgtgaaat cgccggttca tccgtttggt ccgcgacacc aaggaaacgg
     3241 acaggccggt cgcctcggag atgatccggc gtaatgagat aggtaccgta catgctggtc
     3301 tgccagatac tcttcgcggc ctgttcatta ccacccgaga acgcataagc caggcctccc
     3361 acgatgcctc ctccaatggc aaacgccagc ttgaagggag catagaggac ggtcgaaaaa
     3421 aaagcggcag cctgcatacc ggcactggag gcgctgccgc cttccgagtc gcttgccaca
     3481 ggtggggcaa cgggctggcc gtcctggctc caagcaggac caacagccgt gaccgtgacc
     3541 aacgccagga cgatggcgag ggcgacggat cgattccagg ctccatgtga taacaacgaa
     3601 tagaatttca cagaagttcc tccttccaga aaaaaacggg cggcattgcc atgtgatctc
     3661 gtgacgccgt ctagaggcat gattcctaaa taggccgtct gtgggttata gcaaattcaa
     3721 cttcgaatgc aaaccccctg ccgggcagag cctatcaagt ggaaagagtg gcatcgccgg
     3781 gagggaggtc aagttcgtcc aggatgtagg aagtgatgta cgcagcccgc tttatcgctg
     3841 cgtctctgag ttcatcacgg agcacgtcgg cggcaagaca ttggttttct ggtgtgatcc
     3901 cagcttgcaa catcagatcg agcttacacc aacacatcgc ttgcgcgtaa cactcatttg
     3961 cacggttgcg aaaagagagt tggtcttcag aggacaggcc agacaatttc ttctccacaa
     4021 attctgggta ccggagtatc ctggcgatct catccaactg gtcacgcctg acttcaacgg
     4081 cgacttgaat cccgcctttc cagcttcgtt ttttcacgtt aaacacgcag tactgtatgg
     4141 atggttttcc ctccaccggc tccggcccaa aaaagagcgt gacccggtag gaggaggtat
     4201 ccgacgaggc ctcatcagcc atggcgcgca ttgtagcatg attatctcat gctgccgtgt
     4261 gattgacgct ccaaagaggc gacgatatga tgcaagccaa cgatgacgac cgcgcctcac
     4321 caacgggttc tcgacattca gcaggctctt tgcgaagccg gtacgatgga tggctggctc
     4381 ttttacgatt ttcggggaag cgacccactg gcctatcgca ttctattact cgatccaacg
     4441 ttgcacgtca cgaggcgttg gtactattgg gttccagctc aaggcacgcc ggtgaaaatc
     4501 gtgcat
//
LOCUS       DATDNW010000157         5488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1314255, whole genome shotgun sequence.
ACCESSION   DATDNW010000157 DATDNW010000000
VERSION     DATDNW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314255"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VI359_03835"
     CDS             complement(<1..1579)
                     /locus_tag="VI359_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973417.1"
                     /translation="MSPDPVRLALTHLQFPANSSFAQDLEQAGGRVVRQVSGHLVFYR
                     PDGRRFLATDPTGSPLHECEWSQDASGHVHLLRVRMRLDWGQWVGIKPAGLVNETNLN
                     LSHKPGWQRLTADSLRHMAAQAMGVSIEEVRFFYGDDDLVIDARGHAIIRHKKDAFYV
                     LETGGFERTRFMSCMGAMHWDQIDFLPVVELFKSLLPGTGSAAFELIRGLYDDQNQGQ
                     SVQRSLRYRGIPTYPSEAAFRLFSSFFVPHAPPGSDPLTLFMDQARSHQVTWLPASHP
                     PLRYFDDAQRACLTVQGGVLQKVTMADDPTGLPYVNPGGRRTVPFDRSLAIVGDRIVL
                     RDREQERVLVSSQQLQGVTVNADRREISQTDWRSVFVREMPPIRPVEAFGAVLFYPDD
                     DQPIGELAAQPFVADYLQDLAEQDREIGVIVSRAEHILIDNGDAVIATCIPFDRPRDY
                     TVYVHHAAFAQRQAQQLWTISAELQRWDWLRRIRFLTAASVQEDTIQQRYDLVYNWVP
                     LLSHDQPARLSEHVRMVH"
     gene            complement(1580..2911)
                     /locus_tag="VI359_03840"
     CDS             complement(1580..2911)
                     /locus_tag="VI359_03840"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY6973418.1"
                     /translation="MLNPSNQTDVMRDAKESPAEPEVLSRPIPEYVTQLVTRARNAAG
                     RLATLSTSVKNQALTAMADALEEQTEALLAANERDLEAFGTTPDKKAMADRLRLTKAR
                     IAEMAVGIREVAKLPDPVGDMPKMWTRPNGIQVGRVRVPIGVIGIIYESRPNVTADSA
                     ALCLKSGNACVLRGGSEAIHTNTAIANVLSETAEKTGIPAGAITFVERPDRELVPILL
                     KQDRHIDLIIPRGGESLMRIVMEHATIPVIKHDAGVCHTYVDGDADPVMAERVCFNAK
                     VQRPSTCNAMETLLVHQGIARTWLAPFIEKLMTAKVEVRGCQKTCQLSSAVKPASEED
                     FGKEFLDLTLAVKVVKNIDEAMEHIARYGSRHTESIITTDYAKAMRFLREVDASAVLV
                     NASTRLNDGFQFGLGAEIGISTSRIHARGPMGLEELTCSKFIVMGSGQIRE"
     gene            complement(3038..4048)
                     /locus_tag="VI359_03845"
     CDS             complement(3038..4048)
                     /locus_tag="VI359_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY6973419.1"
                     /translation="MRILITGGAGFLGSHLSDLLVENGHSVICMDNLVTGRMSNIAHL
                     LGHERFSFVKYNVCDYLHVEGTVDALLHFASPASPQDYLEFPIATLKVGALGTHKALG
                     LAKAKGARFLLASTSEVYGDPLVNPQPETYWGNVNPLSPRGVYDEAKRFAEAMTMAYH
                     RYHGIDTRIVRIFNTFGPRMRPNDGRVVSNFIVQALQGKPLSVYGDGLQTRSFCYVDD
                     MIRGIFTLLMVDSDKSVEERTNKKFLVAQKADSNRSSMHDPVNIGNPLEVTVLEIAKL
                     VLKLTGSSSAITHQPLPADDPKVRRPDIQRAQLLLGWEPRVPLEEALRRTVEYFRKVL
                     AH"
     gene            complement(4094..4684)
                     /locus_tag="VI359_03850"
     CDS             complement(4094..4684)
                     /locus_tag="VI359_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY6973420.1"
                     /translation="MRTERGVTYVMLMCAIVLIGIVTSIAAKQWKIMVQRELEADLLA
                     RGIEIQQALALYSTAMKAGRVMQGEVYPQTLVDLTRLPKPFLRKVYLDPMAHGEWDYL
                     RSPTGGIMGVRSRSKGKPIRQHEFPLAVRHFEGRSRYDEWIFQYPNPSTASLIPQVPQ
                     GSGSTPSVPLPQGQGGSSGIPSSSSQPVQTQVGSSS"
     gene            complement(4720..5301)
                     /locus_tag="VI359_03855"
     CDS             complement(4720..5301)
                     /locus_tag="VI359_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973421.1"
                     /translation="MTGDRKKILALIVLGLLWGGLLVRQWLTVAEPTRVPLTNVTGLA
                     TATRSLRTPAGGLQVQLDLLAAARTQREMTFTAPRNIFALPRIDGQPIGSASQEVADV
                     NSDEAARQQTVLAALAQFRYLGFIRMEQDRRPNAAMAVLTRNDDVHVVKAGQTVVDQV
                     IVKTITPDSVTLQDRASRLEQMVPLSEETVAQP"
BASE COUNT         1230 a         1572 c         1467 g         1219 t
ORIGIN      
        1 ggtgtaccat cctcacatgt tcgctcagtc ttgccggttg gtcgtgcgac aggagcggca
       61 cccagttata aacaagatca tagcgctgtt ggatcgtgtc ctcttgcact gatgcggcag
      121 taagaaagcg gatacgtctg agccaatccc atcgctgaag ctcggccgag atggtccaga
      181 gttgctgggc ttgcctctga gcaaatgccg catgatggac gtacaccgta taatcgcgag
      241 gacgatcgaa cggaatacac gtggcaatca cggcatctcc gttgtcgatc aggatatgtt
      301 cggcacggga aacaatcaca ccaatctcgc gatcctgctc ggccaggtct tgaagataat
      361 ctgccacaaa cggttgggcc gccagttctc caatcggctg gtcatcgtcc ggatagaaga
      421 gcaccgcacc aaaggcctcg acgggacgga tcggtggcat ctcgcgcaca aaaacagaac
      481 gccaatccgt ctggctgatt tcacgacggt cagcattcac agtcaccccc tgaagctgct
      541 ggctggacac gagtaccctt tcttgctcgc gatctctcaa cacgattctg tctccaacta
      601 tggcaaggct tcgatcgaat ggcaccgtac gcctgcctcc gggattcaca tagggaagtc
      661 cggtcgggtc gtctgccatc gtgacttttt ggagtacgcc accctgcacg gtcagacaag
      721 ctcgttgcgc atcgtcgaag taccgcagag gaggatgcga tgccggcaac catgtcactt
      781 gatgagaccg agcctggtcc atgaacaagg tcaaaggatc actccccggg ggagcgtgcg
      841 ggacaaagaa actgctgaac agacggaacg ccgcctcgga aggataggtc ggaatacctc
      901 gatagcgtag agaccgttga accgattgac cctgattctg atcgtcatag agaccgcgga
      961 tgagttcgaa tgccgccgaa ccggtaccgg gcaacagcga tttgaagagc tcgactactg
     1021 gcaagaaatc aatctggtcc caatgcattg cgcccatgca ggacatgaat cgcgttcgtt
     1081 caaatccccc ggtttccaat acgtagaagg catctttctt atggcggatg atggcgtggc
     1141 ctcttgcatc aatcaccaaa tcgtcatccc catagaagaa ccgaacttcc tcgatggaaa
     1201 cccccatagc ctgcgccgcc atgtgtcgga gactatccgc tgtgagtcgt tgccaccctg
     1261 gcttatgtga aagattaagg ttggtctcat tcaccaaacc agccggctta atgccaaccc
     1321 attgccccca atcaagccgc atccgcaccc gcagaagatg gacatgaccc gatgcatcct
     1381 gactccactc acattcatgt aatggactgc cggtcggatc agtcgcgaga aaccgtcgac
     1441 cgtctggacg ataaaaaacg agatggccgg agacctggcg cacgactcgt ccgccggctt
     1501 gttcgagatc ttgcgcaaag gaggaattgg cagggaactg aagatgagta agggcgagac
     1561 gaacggggtc gggcgacatc tactcgcgga tttgcccgct ccccatgaca atgaacttgg
     1621 agcaggtcaa ttcttccagt cccatcggac cacgggcatg aatgcgtgac gtactgatcc
     1681 cgatttccgc tcccaacccg aattggaagc cgtcattgag acgagtggac gcattcacga
     1741 gcacggcgct ggcgtcgact tctcggagaa atcgcatcgc tttggcgtag tcggtcgtga
     1801 tgatggattc cgtgtggcgg gacccgtacc gagcaatgtg ttccatcgcc tcatcgatgt
     1861 tcttcacaac tttgacggcc aaggtcagat ccagaaactc cttgccgaaa tcctcttcgc
     1921 tcgctggctt gacggcagag gacaattggc aagtcttctg acagccgcgg acttcgactt
     1981 ttgccgtcat aagcttctcg atgaacggcg caagccaggt tcgggcaatc ccctgatgaa
     2041 ccaagagcgt ctccatcgca ttgcatgtgg aaggtcgctg gaccttggca ttgaaacaga
     2101 ctcgttccgc catgactgga tcggcatcgc catccacata tgtatggcag acaccggcgt
     2161 cgtgcttgat gacggggatt gtcgcgtgtt ccatgacaat cctcatcaga gattcgccgc
     2221 ctcggggaat aatgagatcg atatggcgat cttgtttcag gagaattgga acgagctctc
     2281 gatccggccg ttcaacaaac gtgatggcac cggccggaat gccggtcttc tccgccgttt
     2341 ctgacagcac gttggcaatc gccgtattgg tgtggatcgc ctcacttcca ccacgcaaga
     2401 cacaggcgtt gccggacttc agacagaggg ctgcggaatc cgcggtcacg ttcggacgcg
     2461 attcatagat gatgccgatc acgccgatgg gaacccgcac tcgtcccacc tgtatgccgt
     2521 taggccttgt ccacatcttg ggcatatcgc ccacaggatc tggaagtttc gcgacttccc
     2581 gtatgccaac tgccatctcc gcaatccgcg ccttcgtcaa tcgaagacgg tccgccatcg
     2641 ccttcttgtc cggcgtagtc ccgaatgcct ctaagtcccg ttcgttggca gcgagcaacg
     2701 cctcggtctg ctcttctaac gcatcggcca tcgccgttaa ggcctgattt ttcaccgatg
     2761 tcgaaagagt ggccaaccgg cccgcggcat tcctcgcacg tgtgactaat tgggtaacgt
     2821 attccggaat cggcctgctc aaaacctccg gctcagcagg agattccttc gcatcccgca
     2881 tcacatcggt ctggttgcta gggttcaaca tgtgagagcc tcaagatccc tcttcggtga
     2941 cagcagcaca ggataccgtg ggagtttcag acgggtcaag ccacagcact gttcgtcgac
     3001 cgaccaatgg aattgtcatc tgaagggaaa aagaacgcta atgcgccagt accttgcgga
     3061 aatattcgac agttcgacga agggcctctt cgagcggtac tcttggctcc catcctagga
     3121 ggagttgggc acgttgaatg tcaggtcgtc tgacttttgg atcgtcggcg ggtaacggct
     3181 ggtgcgtgat ggcgctggag gagccggtca gtttgaggac aagtttggca atctccagaa
     3241 cagtcacctc gagtggattg ccgatgttga ccggatcatg catgctggac cgattggaat
     3301 cggccttctg cgcgacgaga aacttcttgt tggtccgctc ttcgacgctt ttgtccgaat
     3361 ccaccatgag gagagtgaag atgccacgga tcatgtcgtc gacatagcag aaacttctgg
     3421 tttgtaatcc atctccatag acgctcaatg gtttcccctg caatgcctgc acgatgaaat
     3481 ttgataccac gcgaccatca ttcgggcgca ttcggggacc gaaagtgttg aagattctca
     3541 cgatcctcgt atcgatccca tggtagcgat gataagccat cgtcatggcc tcagcaaacc
     3601 gtttggcttc gtcgtataca ccacgcgggc tgagcggatt gacattcccc caataggtct
     3661 ccggttgagg gttcacaagg ggatcaccgt agacttcgga ggtgctggcc aagagaaatc
     3721 gtgccccctt ggctttcgcc aacccaagtg ctttgtgagt tcccaacgct ccgaccttta
     3781 gcgtcgcaat cgggaactca agataatcct gcgggcttgc aggcgacgcg aaatgcaaga
     3841 gcgcatcgac tgtcccttca acatgaagat agtcgcagac gttgtacttc acgaatgaaa
     3901 accgttcgtg accgagcaga tgggcgatgt tgctcatccg gccggttacc agattatcca
     3961 tgcagatgac ggaatgtccg ttctcgacca aaagatcgga cagatgactt ccaagaaatc
     4021 ccgccccacc ggtgatcaga atcctcatcg gagtccgtcc ttcactcgtg ccccatacag
     4081 acagacacgc ccattaggaa cttgatccca cttgcgtttg caccggctga cttgaggacg
     4141 agggaatgcc cgagctgcca ccttgtccct gcggcaacgg aacggaggga gtcgacccgc
     4201 tcccttgagg aacttgagga atcagactgg ccgttgaagg attaggatac tgaaagatcc
     4261 attcgtcata cctggatcgt ccttcaaaat gccggaccgc gagaggaaac tcatgttgcc
     4321 gaatcggctt ccccttgctt cgacttcgca cccccataat gcctcctgtt ggagaccgga
     4381 gatagtccca ttccccatgt gccatggggt ccaggtagac ttttcgcaga aatggcttcg
     4441 gaagtcgagt gaggtccact aacgtttgag gatagacttc tccctgcata acacgtcccg
     4501 ctttcatcgc cgttgaatac agcgccagcg cctgctgtat ctcgatcccc cgagccagga
     4561 gatccgcctc taattctcgc tgcaccatga ttttccactg cttggctgcg atactcgtga
     4621 cgatgccgat cagtacgata gcgcacatca gcattacgta ggtcacacca cgttcggttc
     4681 tcataccagc tcgcatgcct cgcgttgaca taggggccat caaggttgcg ctacggtctc
     4741 ttccgagagg ggaaccatct gttcgagacg ggatgcccga tcctgaagcg tgacgctgtc
     4801 gggcgtaatg gttttgacga tcacctgatc aacgacggtc tgtccggctt tgaccacgtg
     4861 tacatcatca ttccttgtca gtaccgccat cgcagcattg ggtcgccgat cttgctccat
     4921 ccgtatgaat ccgagatagc ggaactgtgc gagtgcggcg agtacagttt gctggcgcgc
     4981 ggcttcatcc gagttcacat cggcaacttc ctggcttgca ctaccaatcg gctgaccatc
     5041 aatacgaggc agagcgaaga tgtttcgtgg agcggtaaag gtcatttccc gttgagtccg
     5101 tgcggccgca aggagatcaa gctgaacctg caatcctccc gcaggagtcc gtaacgacct
     5161 tgtggcagtg gcaagccctg tcacattcgt caacggaact cgagtcggtt ctgccaccgt
     5221 caaccattgt ctgacgagca accctcccca gaggaggccc agaacgatga gggccagtat
     5281 ctttttccga tcccccgtca taaccaatgc ctactgaacc gtgacaccgc ccggttcgtt
     5341 ccgtaagaag gtcgagatct tgatgttgaa tgtcagcgtc tgttcctgtt ggttggccgg
     5401 gcgcactagt tcgagatcct cgataatgat ccactcctct gcggtctcca gttcgtagag
     5461 aaaccgacga agatcttcgt agcgaccg
//
LOCUS       DATDNW010000158         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_15763,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000158 DATDNW010000000
VERSION     DATDNW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15763"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VI359_03860"
     CDS             complement(<1..1224)
                     /locus_tag="VI359_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY6973422.1"
                     /translation="MNSNVCIKRPLHLRGLELMLLASLFAGCVTPVSAEVASERPSEQ
                     RVRARDLGLILGQYQPGPLNAITDVAGVKVGHATLIKGDGPLKPGDGPVRTGVTVIIP
                     RDDVWHKKVPAGSFVLNGTGEMTGLSWVTESGFLEYPVALTNTLNVPRVANGIMSWMI
                     RQYPEIGITDDTLTPVVAECDDGRLNDIQGRHVSEADVAKALDGAMSGPVPEGTVGAG
                     TGMVSYGFKGGIGTASRRFSERDGGYTLGVMVNANHGRRPELIMGGIPVGQLYDGTSV
                     RTSDVSDGRTTGASEGSIIVVIATDAPLDGRQLTRLAKRAALGLARTGSTARHGSGDF
                     ILAFSTGNVIPHYPKEQTYSATHLADTYLNPLITATVEATEEAILNALTMATSVTGRD
                     GNRVEAISLDRLRSLI"
     gene            complement(1214..>2489)
                     /locus_tag="VI359_03865"
     CDS             complement(1214..>2489)
                     /locus_tag="VI359_03865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY6973423.1"
                     /translation="HQNRRLGFDPKSIGAVLLSHAHIDHSGALPVLAKHGFSGKVHMT
                     PATGDLTTVMLEDSAHIQKYDCMYVNKKERRRGKERIQPFYDTDDVRKIARHFHSARY
                     EDTIKIAPRITASFHDAGHILGSAAVRLKYSHRGNTTTILFTGDLGRKSMPILRDPAP
                     PPPCDILITESTYGDRLHEQSRENMTAKAEEIVTYIKKTGGKIIVPAFAVGRTQEVVM
                     RIKDLVSESRIDPLPIYIDSPLASKATEIFRRHPECYDEETTKTFSADGDPFAARYIH
                     YISSVDDSKRLNSAKGPCVIIAASGMCEGGRVLHHLKHAIQDEANVIAIVGFQAEHTL
                     GRRLVEGWDVVPIFGKQTPRRARVVRFNGFSAHADRNELLDYVRSVTPSPEKVFVVHG
                     EEKQALSLASAIQSEHAGMEVVVPQQDSVHEL"
BASE COUNT          525 a          709 c          713 g          542 t
ORIGIN      
        1 aatcaaggac cggagccgat ccaacgaaat cgcctcgaca cggttcccgt cgcgcccggt
       61 caccgaggtg gccatggtca acgcattaag gatcgcttcc tccgttgctt cgacagtggc
      121 ggtaatcaga ggattcagat acgtatctgc gaggtgagtg gccgagtacg tctgctcctt
      181 gggatagtgc gggatgacgt taccggtcga gaaggccagt atgaagtcgc cgctgccgtg
      241 tcgtgccgtg gagccggttc gtgccaggcc gagcgccgcg cgtttggcga ggcgcgtcaa
      301 ctgccggccg tcgagtggtg catcggtggc gataactacg atgatcgagc cttcgctagc
      361 cccggtggtt cgtccatcag acacgtcgct ggtgcgtacc gatgttccat cgtaaagttg
      421 gccgacggga attccaccca tgataagttc tggtctccgt ccatggttcg cgttgaccat
      481 aaccccgagg gtatatcctc cgtctctttc agaaaaacgc cgggaagctg tgccaatccc
      541 gcccttaaat ccataggaca ccatcccggt cccggcgccg actgtgcctt ccggaacagg
      601 tccagacatg gccccatcga gtgcttttgc cacatccgct tccgaaacat gccgtccctg
      661 gatgtcattg agtcgtccgt cgtcgcattc agccacgacg ggcgtcaacg tatcgtcggt
      721 aatcccgatc tccggatact gccgaatcat ccagctcatg atgccattgg cgacgcgcgg
      781 cacgttcaag gtgttcgtca acgccacagg gtattcgaga aatccggact cggtaaccca
      841 cgagagaccg gtcatttccc cagtcccatt cagaacgaac gaccccgccg gtactttctt
      901 gtgccagacg tcgtcgcgag ggatgatcac cgtcacgccg gttcgtaccg ggccgtcccc
      961 gggctttagt ggcccatcgc ctttgatcaa tgtcgcatgg ccgaccttca cgccggcgac
     1021 atcggtaatg gcattaagcg gaccaggctg atattggccc agaatgagac cgagatcgcg
     1081 ggcgcgaacc cgttgctctg agggacgttc cgatgcgact tccgcactca ccggcgtgac
     1141 gcaaccggcg aagagggagg cgaggagcat aagctctaag ccacgaaggt gtaacggtcg
     1201 tttaatgcag acgttagagt tcatggacag agtcttgttg cggcaccaca acctccatcc
     1261 cagcatgttc gctctgaatg gcgctggcca acgacagggc ctgtttttct tcgccatgaa
     1321 cgacaaacac tttctccgga gatggagtca ccgaccggac atagtccagc agctcattcc
     1381 ggtcagcgtg ggcagaaaac ccattgaagc gcaccacccg tgcgcgtcga ggtgtctgtt
     1441 tcccaaaaat cgggacgacg tcccatcctt cgaccaatcg gcgaccgagt gtatgttcag
     1501 cttgaaagcc gacgatcgcg atgacattgg cttcgtcttg gatcgcatgc ttgaggtggt
     1561 gcaggacccg accgccttcg cacatccccg aggcagcaat gatgacgcag ggaccttttg
     1621 cgctgttcag gcgcttgctg tcatcgacgg aggatatata gtggatgtag cgtgcggcaa
     1681 agggatcgcc gtctgcggag aaggtcttcg ttgtctcctc atcgtagcat tcaggatgtc
     1741 gacggaagat ctcagtggct ttcgaagcta acggcgaatc gatgtaaatc ggtaagggat
     1801 cgattcggct ttcagagacc aaatccttga tccgcatcac cacttcctga gtccgcccaa
     1861 cggcaaaggc cggcacaatg atcttcccgc ccgttttttt gatgtaggtg acaatctcct
     1921 ccgcctttgc agtcatgttc tcgcggctct gttcgtgcaa tcggtcgcca taggttgatt
     1981 cggtaatcag gatatcgcag ggaggaggag gtgccggatc gcgcaagatc ggcatcgact
     2041 tccggcccag gtcaccggtg aaaaggatgg tagtcgtatt tccgcgatgc gaatatttga
     2101 gccgtaccgc ggccgaaccc aagatatggc cggcatcgtg aaaggaggcc gtaatgcgcg
     2161 gagcgatctt gatcgtatcc tcgtaacgag cgctatggaa atgtcgcgcg atcttacgca
     2221 catcgtccgt atcgtagaat ggctgaatgc gctctttgcc tcgtcgacgc tctttcttgt
     2281 tcacatacat acaatcatac ttctgaatgt gagccgaatc ttccagcatg acggtggtga
     2341 gatcgccagt agcaggggtc atgtggacct tgcctgaaaa cccatgtttg gccaagaccg
     2401 gcagagcgcc ggaatggtcg atatgggcgt gagataacag cacagcccca atggatttgg
     2461 gatcgaaacc aaggcgacga ttctggtgc
//
LOCUS       DATDNW010000159        10156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_437040, whole genome shotgun sequence.
ACCESSION   DATDNW010000159 DATDNW010000000
VERSION     DATDNW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437040"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VI359_03870"
     CDS             complement(<1..818)
                     /locus_tag="VI359_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973424.1"
                     /translation="MELRFQPALLQEVIDSFVEKTEREGDPTYYKEFHELADPIYEKF
                     TLDDRESEFKKLYQYLFGIWGFSDIIRDAFNEYPVLQKSIGIVLVKGVLKEDQEGVDI
                     LRKWGSVEQELAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELMHISDMLDPLFGY
                     DPDTKVGQNPGEETLILHRYRVLWSISVDSRLISASKEPMLPKEDRFKEFRSWYRKIP
                     APQLKSVFEGLWQTGFFTHSELIEMATDTLRVMDRAVDVEGGEVPDTANKVMLMP"
     gene            1034..2137
                     /locus_tag="VI359_03875"
     CDS             1034..2137
                     /locus_tag="VI359_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY6973425.1"
                     /translation="MADPVTTTPIAPTPSPSSAGPGVTTQPVDCLEYWKTDAREIRTF
                     RGHSHGVWSVAIAPDGLTLASGGVDRLVRIWEIETGRLLRSLRGHTNDIRAVVYTPDG
                     QTLATGSEDRTIRLWNPRTGEPLKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIKIW
                     EVTTGTELMTLLGKDQYDHHWSPCVAFSPDGIHLASGSDIGKIKLWEVLPSGEEKVLH
                     NGHWKEDEEDSTETRGYYIEDDGGFQKPMDYWIGAMTFTPDAKTLITGSRDTTIKLFD
                     LPNVVERRTLTGHGGWVRSLVVTPDGKVLISASDDQTIKFWDLSTGRVFRTLKGHTGG
                     VRAIALSPEGTRLASAAWDRTVKLWAGGSEPTE"
     gene            complement(2303..3745)
                     /locus_tag="VI359_03880"
     CDS             complement(2303..3745)
                     /locus_tag="VI359_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973426.1"
                     /translation="MKGLRILIVDDEPLMRLSMMDALEAVGYDVQAAASGTEGIEAIR
                     QRDFDLVITDLRLPGTDGLSVLKATKDKAPHTDVVVITAHGSVETAVGAMKLGAFDYI
                     TKPFQMDELLLIVERAGKVVALRRENQDLKETLEDKFSFGGILGTNSQMRAVLDKIKL
                     VATTDSTVLILGESGTGKELVANAIHQNSPRSHYPLIKVSCAALPETLLEAELFGHEK
                     GAFTGALRQRRGRFELAHKGTLFLDEIGEISPVVQVKLLRVLQERKFERVGGNDTIDV
                     DVRLVCATQKDLRKEVAQGRFREDLFYRLNVVQIVVPPLRQRQEDTMLIADHVLNTCS
                     VRMNKKLRGFSQQARELLLRYSFPGNVRELENMVERAVALGRDRDAIQPSDLCGFQSC
                     PFTGGAPQESCGFCSEGLSGRQKQHAQPLTSLAAAREQFEREYIVSVLERVEGSRTTA
                     SKILGLSRKALWEKCKRYKIPSAKDGPDED"
     gene            complement(3742..6921)
                     /locus_tag="VI359_03885"
     CDS             complement(3742..6921)
                     /locus_tag="VI359_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6973427.1"
                     /translation="MKFWSRSHSLQRKIITAIVVVGLLPLTLLLALTYHEERRSLQVT
                     TGANFKEVAVEASRRIEMHISRGMSEAQQLATTPFLRTAVTEANRTYEDKDPTTIQGM
                     IKDWQQRWKQRDKHSEFPLFINRIVTNYLIRWHDIRKADYVGIFVTDNQGALVISSIP
                     QVEYFYGKTAWWQAIVKSKTPHVYVSDIAFDPNFGTHVVVVAAPILDDTQHVAIGAIT
                     ILLRRDTLFHSISEVTVGSTGHAMLFNSDGVPVICPVLSLEEHTVTPELIATINQAKS
                     GWAVASDDSHGSRNSLIGFAPVRFGESLAPGSIGGKHWITVVRQDPRETYAPLADLVA
                     KVLLYGLVVLAVLAGTGVVAARRIARPIQLLHDGVREIGSGRWDQRLDLKTGDEIEHL
                     AEAFNRMAANLKVSFAQIEQRMAEVHRLEEKYRDLIEHSPEMIYQLNRGGQFVHVNKT
                     GLDKLGYTLDEMLAMRLWEIVPKGQEALVLNYLERLMSQGRSSIETILISKDGRPIDV
                     EIHATALFDQERGGLVHSRAFVRDITERRQLERQLQQYTTKLEQAVSERTQQLVASQA
                     RYKALFDLVADSVFMVDAQGVVVAVNKREEQALGYAESMVVGKSLFDVAMPEHQEVLR
                     ELLTKLMAGQRQVPTKEITVRHATGQEIPVEMDLIHVGATESPLVMVQLRDITDRKRL
                     ERQLQTYREELELKVRERTREIEETKQYLENLLENANDVIYTLDTDQHFTYVNSKIEA
                     WGYRKDDLIGRPYLSLLSKRHRGRRLKNTLDIGAKQVYEVEVVTRMGEARTVMVSVSP
                     LHGIEGTILGVLGIARDMTETKKLEQQIRNSEKLASVGKLAAGVAHEVNNPLAGILNC
                     IYNLRKGTLSPTRQEEYVASMEDGVRRVQKIVRQLLDFSQQHEPAFVPADINQVIDRV
                     LVLTTHLFAPNRIVLETMLGHGLPSLMVDRHMIEQVLMNLILNAVQAMKGGGVLTIRT
                     SVLEGVCLVEVRDTGSGIAPAVLPKIFDPFFTTKGEGEGTGLGLSVSLGIVERHGGKI
                     LVESEVGKGTTFTLCLPLSRDRSFVEKTS"
     gene            complement(6976..7893)
                     /gene="secF"
                     /locus_tag="VI359_03890"
     CDS             complement(6976..7893)
                     /gene="secF"
                     /locus_tag="VI359_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY6973428.1"
                     /translation="MLEILGKTNFDFMSKRHLAFTFSGVMVLLGIIAVVQIARGAANL
                     GIDFAGGTAVQLKFDQPIHIDVARKALEANGLGDAELQEFGQENKLLIRVKASTTIEE
                     KVAERVVGVFTKEIPSNHFVVESSTEIGPTIGKKLQEDALIAVVISFAGIILYIAARF
                     EFRFGIAAALATFHDVLAVLGAFYLLDKEITLLVVTALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRRDSEETMINNAVNQVLSRTIVTSLTVVLVLIPLTVAGGEVLHDFSLALLWGV
                     IFGTYSSVFVASPLLLLWPGTPGRLLKRS"
     gene            complement(7909..9552)
                     /gene="secD"
                     /locus_tag="VI359_03895"
     CDS             complement(7909..9552)
                     /gene="secD"
                     /locus_tag="VI359_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY6973429.1"
                     /translation="MKRVGGRLLLLSLVIIVSLVFFVPTYRPLFQALPDWARKLLPDK
                     GITLGLDLQGGIHLVMEVDEDRAVEIALDRTVVSLQDLLVDKKIPVESVKRTGLNRIT
                     ITFQNPELKDQIKKLIDDYPTFTEAESAGSSDTLIWDLRDTEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLVQRQGLKQIVVQLPGVKDPKRAKDLIKETALLEFKMLDEDNQLKL
                     DLPARVPKDREADVLKQAEAKLPEGDQILFERVVEKDTGREYRIPYVVKKRVMLTGDV
                     LSDARVAIGQFNDPYVSITFDAKGGKEFDRITGENVKKRMAIVLDNSIYSAPVIQERI
                     TGGRAQITGTFSTQEANDLAIVLRAGALPAPLRIIQDLTVGPSLGQDSIDKGVQATLI
                     AGAMVVVFMIVYYKLSGVVADFALILNLVCLIGALSALNATLTLPGIAGIVLTIGMGV
                     DSNVLIFERIREELRQGKPVRLAVDGGYEKALLTIVDSHVTTLITGVALFLFGTGPIK
                     GFAVTLCLGIAINLFTALVGTKVIFDIVNQRQKVEQLSI"
     gene            complement(9583..9915)
                     /gene="yajC"
                     /locus_tag="VI359_03900"
     CDS             complement(9583..9915)
                     /gene="yajC"
                     /locus_tag="VI359_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY6973430.1"
                     /translation="MVMQSVAWAQGSTGGGGGMGSLLSLVPFVLIFVIFYFLLILPQQ
                     KRQKQQKTMMEALKKGDKIVTASGIWGTIANLGKDTVTLQIADNTKIKIQREHIARIR
                     GDEDDKES"
     gene            complement(9941..>10156)
                     /locus_tag="VI359_03905"
     CDS             complement(9941..>10156)
                     /locus_tag="VI359_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973431.1"
                     /translation="FNVKEMLGSRLNTIHNLWYFADLMRRIRVAIEQGTFSAFRAEFY
                     RSRGEMETDEVLNSTGDVDDSRRTRQD"
BASE COUNT         2229 a         3042 c         2595 g         2290 t
ORIGIN      
        1 ggcatgagca tgactttatt ggccgtgtcg ggaacttcgc ctccttcgac atccacggct
       61 ctgtccatca cacgcagcgt atccgtggcc atttcgatca actctgagtg ggtaaagaag
      121 ccggtctgcc agagtccctc gaagacggac ttcaattgag gggcaggaat tttccgatac
      181 caggagcgaa attccttgaa gcgatcttcc ttcggcagca tgggttcttt gctggcggat
      241 atcaggcgac tgtccacact gatgctccag agcacacgat atcggtggag aatcagcgtc
      301 tcctcaccgg ggttttgtcc caccttcgta tcagggtcat acccaaacag cggatccagc
      361 atgtcggaga tatgcatgag ttcatgacgg cagtaccgag tcagggcggg atcgtagaat
      421 cggcgcggaa tgagcttgat gccgacgcct ttgagcccct tctcctcaaa ctgtttggct
      481 aactcctgct cgaccgagcc ccatttcctc agaatatcaa caccttcttg atcctctttg
      541 agcactcctt tgaccaagac aatcccgatg ctcttctgga ggacaggata ttcattgaat
      601 gcatcgcgga tgatgtccga aaatccccag atcccgaaga gatactggta gagtttcttg
      661 aactcgcttt cgcgatcatc gagggtaaac ttctcgtaaa tcggatcggc caattcgtgg
      721 aattctttat aataggtagg gtccccctcc cgttccgtct tttcgacaaa cgagtcgatg
      781 acctcttgaa gcaatgcagg ttgaaagcga agttccataa tcgtcctcaa tcacttaccc
      841 tagcgtggaa ggctccgaac gtcgctcgac agaccggcga acccgccccg atcgccgtct
      901 tgacttgacg actgagcatc tcttacgatc acgactgacc gtagcgaaat tatactggtc
      961 cccattgctg ttttgcaatc aagctagggc atggcccggg agtatatgcg tttcgttcaa
     1021 aggaagattc accatggctg accctgttac cacaaccccg atcgcaccaa ccccttcccc
     1081 aagctctgca gggccgggag tgaccacgca accggtcgat tgcctcgaat attggaagac
     1141 cgacgcgcgg gagattcgaa catttcgagg ccactctcat ggagtctggt cggtggccat
     1201 tgcaccggac gggttgaccc ttgcgagcgg aggcgtcgat cgtctcgtcc gcatttggga
     1261 aattgaaacg gggcggttgc tccgttctct caggggacat acgaacgaca tccgggcggt
     1321 ggtctatacg cctgatggac agaccctcgc gacgggaagt gaggatcgaa ccattcgact
     1381 ctggaatcct cgaaccggcg aaccgttgaa actgctcttc acccggtatg atcacaacgt
     1441 gtgcagtctc tctctgtcgc cagacggcct catgctggcc agagggagcc ataataaaga
     1501 catcaagatt tgggaagtca cgaccggcac cgaactcatg acgttgctcg ggaaagatca
     1561 atacgaccac cattggtcgc cttgcgtcgc attttcgcca gacgggatcc atctcgccag
     1621 cggaagtgat atcggcaaga tcaagctctg ggaagtcctc ccgagcggag aagaaaaagt
     1681 gctccacaac ggtcactgga aagaggatga ggaggactcc acggaaacac gcggatatta
     1741 catcgaggac gatggcggct tccaaaagcc gatggattat tggatcggcg caatgacctt
     1801 tacgcctgat gccaagaccc tcatcacggg gagccgtgac accacgatca agctcttcga
     1861 cttaccgaac gtggtcgagc ggcgcacctt gacagggcac ggaggatggg tccgttcgct
     1921 cgtcgtcact ccagacggca aggtgctcat cagcgccagc gacgatcaga cgatcaagtt
     1981 ctgggacctc tcgaccggcc gcgtgttccg taccctgaag ggacataccg gtggtgttcg
     2041 tgcgatcgcc ctctcacctg agggcacgag actggccagc gcggcctggg accgaacagt
     2101 aaagctttgg gccggaggct cagagcccac cgagtagtac ctgacaagct gcgggtactt
     2161 ggtctggcca gaagttcgca ggatgctcaa aaaggccttc cagcaaggcc gcagtgagtg
     2221 aaggtccgag gcgtaccctc tggggtacgt tgagggtctg aacgatatga gaatgcagct
     2281 ggaggacttt ttcagcatcc tgtcagtctt cgtccggccc gtccttcgcc gacggaattt
     2341 tgtaccgctt gcatttttcc cagagggcct tgcgcgaaag ccccaggatc ttcgacgccg
     2401 tcgtgcgact tccttcaact cgttctaaca ccgacacaat atactcgcgc tcgaattgtt
     2461 cgcgcgccgc ggccaacgag gtcaagggct gtgcatgctg tttctgccgg ccggagagtc
     2521 cctcactgca aaagccgcac gattcctgtg gcgccccacc ggtaaacgga caggattgga
     2581 acccgcagag gtcggacggt tggatcgcgt cccggtctcg tcccaacgcc actgcacgct
     2641 cgaccatatt ctccagctcc cgaacgttcc caggaaacga atagcgtaag agcagttccc
     2701 tggcctgttg cgaaaatcct cgcaacttct tgttcatcct gaccgagcag gtgttcagaa
     2761 catggtcggc gatcagcatg gtgtcttcct gccgctgacg cagcggtggc acgacgattt
     2821 gcacgacatt gaggcgatag aagaggtctt cacgaaagcg gccttgggcc acctctttgc
     2881 ggagatcctt ttgcgtcgcg cagacgagcc gcacatcgac atcgatggta tcgttgccgc
     2941 cgacccgctc aaactttctc tcttgcaaga cgcgcaacag cttgacttgc acaacgggag
     3001 aaatttctcc gatctcgtca aggaacagcg tgcctttatg cgccaactcg aaccgtcccc
     3061 gccgctgacg aagcgccccg gtgaaggccc ctttctcatg gccaaagagt tcggcttcga
     3121 gcagcgtttc aggaagcgcc gcacagctga ccttaatcaa tggatagtgg cttcgtgggc
     3181 tattctggtg aatagcgttg gcaaccaatt ctttccccgt cccgctttca cccagtatca
     3241 agaccgttga atcggttgtc gccaccagct tgattttatc gagcaccgcc cgcatctggc
     3301 tgttggtccc cagaatccct ccgaagctga acttgtcctc caacgtctcc ttcagatcct
     3361 gattttcccg tcgcagggcg accaccttgc cggcccgctc gacgatcaac aacaactcat
     3421 ccatttgaaa cggcttcgta atgtagtcga aggcgccgag cttcatcgct cccaccgccg
     3481 tctcgaccga tccatgcgcc gtaatcacca ccacgtccgt atggggagcc ttgtccttgg
     3541 tcgccttgag caccgacaag ccatccgtac cgggcagacg taagtctgta atgacgagat
     3601 cgaagtcccg ttggcggatc gcctcgattc cttccgtccc ggatgccgcc gcctggacgt
     3661 cgtagcccac cgcttccaaa gcatccatca tcgaaagccg catcaacggc tcatcgtcga
     3721 cgatcaggat cctcagtccc ttcatgacgt cttctccacg aaagagcggt cgcgggaaag
     3781 gggcagacac agagtaaacg tcgtgccttt cccgacttcg ctctccacga gaatttttcc
     3841 tccatgccgt tccacgatcc cgaggctgac cgaaagaccc aaaccggtcc cttcgccttc
     3901 gcccttggtc gtgaaaaacg gatcgaagat tttcggaagc acggccggag caatgcccga
     3961 tcccgtatca cggacctcaa cgagacacac gccttccagc acagacgtgc gtatcgtcaa
     4021 gacacctcca cctttcatgg cttgcaccgc gttgagaatc aggttcatca ggacttgctc
     4081 gatcatatgc cggtcaacca tcaggctcgg caatccatga cccagcatgg tttccagcac
     4141 gatccgattg ggggcgaaca aatgcgtggt caaaaccaag acccgatcga tgacctggtt
     4201 gatgtcggca ggaacaaagg ccggctcatg ctgctgggag aaatcgagca gctgtcgaac
     4261 gattttctgc acgcggcgca ccccatcctc catcgacgcg acatactcct cttgtctcgt
     4321 cggagagagg gtacctttgc ggagattgta gatgcagttc aaaatcccgg cgagcggatt
     4381 attgacttcg tgcgccactc cagcggccag cttcccgacc gaggccaact tttctgaatt
     4441 ccgaatctgt tgttccaatt tcttggtctc cgtcatatct cgggcgatac ccaagacccc
     4501 gaggatcgtc ccttcaatcc cgtgaagcgg ggaaacactg accatgacag ttctcgcctc
     4561 tcccatgcgt gtgacaactt ccacctcata gacctgcttc gctccaatgt cgagggtatt
     4621 cttcagacga cgcccccgat gtcgcttcga taacaatgag agatatgggc gcccgatcaa
     4681 atcgtccttg cgatagcccc acgcctcaat cttactattt acataggtaa agtgttgatc
     4741 cgtgtccaag gtatagatga cgtcattcgc attttcgagc aggttttcga gatattgctt
     4801 ggtttcttcg atctccctcg tccgttcacg gaccttgagt tccaattcct ctcgataggt
     4861 ctgcaattgg cgctccagcc gttttcgatc cgtgatatcg cgcagctgga ccatcaccaa
     4921 cggactctcc gtcgctccaa cgtgaatgag atccatttcg accggtatct cttgtccggt
     4981 ggcatgtcgc acggtgattt ctttggtggg cacctgccgc tgtcccgcca tgagtttggt
     5041 caacagctcg cgcagcacct cctgatgttc gggcatcgcc acatcgaaca gactcttccc
     5101 caccaccatg gattccgcat agccgagcgc ttgctcttcc cgcttattca ctgccaccac
     5161 gacgccctgc gcatccacca taaacacgga gtcagccaca agatcaaaga gggccttata
     5221 gcgcgcctgc gaggcaacga gctgctgggt tcgctccgac accgcttgtt ccagcttggt
     5281 cgtatattgt tggagctgcc gttccaattg gcgccgttcc gtgatgtcac gaacgaaggc
     5341 gcgcgaatgc acgagcccgc cccgttcctg gtcaaacaag gctgtcgcat gaatctcgac
     5401 atcgatcggt cgaccatctt tcgagatcaa aatcgtctcg attgaactcc gcccctggga
     5461 catgaggcgc tccaggtaat tcaacaccaa ggcctcttgc cctttcggca caatctccca
     5521 cagccgcatc gccaacattt cgtccagggt ataacctaat ttatcgaggc ccgttttgtt
     5581 cacatggaca aattgaccgc cgcggttcaa ctggtagatc atttctggag aatgctcgat
     5641 gaggtcgcga tacttttcct caagacgatg gacctccgcc atgcgttgtt caatctgagc
     5701 aaaagagact ttgagattcg ccgccatccg attgaacgcc tcggcgaggt gctcgatttc
     5761 atcgcccgtt ttgagatcga gtcgctggtc ccaccggcca ctcccgattt ctctcacccc
     5821 gtcatgcagg agctggatcg gacgggcgat ccggcgcgcc gccactacgc cggtccccgc
     5881 gagcaccgcg agcacgacca atccgtacaa caacaccttg gccaccaggt cggcgagggg
     5941 cgcataggtc tctcgtggat cttgtcgcac gacggtgatc cagtgtttcc caccgatgct
     6001 gccgggcgct aggctttccc cgaaccgaac cggagcaaac ccgatcagcg aattacgact
     6061 cccatgagag tcgtccgaag caacagccca tcctgatttc gcctgattga tggttgcgat
     6121 caattccggc gtgaccgtat gttcttcgag ggagagaacc ggacagatga ccggcactcc
     6181 atccgagttg aacaacatcg catgcccggt cgagccgacc gtgacttcgg aaatggaatg
     6241 aaacaacgtg tcccgtctca acaaaattgt tatggcgcca atggcgacat gttgcgtgtc
     6301 atccagaatc ggagccgcca cgacgactac atgggtgccg aagttgggat caaaggcgat
     6361 gtcgctcaca tacacatgag gcgtcttgga cttcacgatc gcctgccacc aagccgtttt
     6421 cccgtagaaa tattccactt gcgggatcga actgatgacg agagctccct gattgtcggt
     6481 cacgaagata ccgacgtagt cagctttccg aatgtcgtgc cagcggatca aataattggt
     6541 gacgattcgg ttaatgaaca gggggaattc gctatgtttg tcgcgttgct tccaccgttg
     6601 ttgccaatcc ttgatcatgc cctggatcgt ggtgggatct ttgtcctcat aagtccggtt
     6661 ggcttcggtc accgccgtcc ggagaaacgg cgtcgtcgcc agttgctgag cctcactcat
     6721 cccgcggctg atgtgcatct caatccgacg cgacgcttca accgcaactt ctttgaagtt
     6781 cgcccccgtg gttacttgaa gcgagcgccg ttcttcatga taggtcaaag ccaggaggag
     6841 ggtcagtggc aggagtccga ccaccacgat tgccgtgatg atcttacgct ggaggctgtg
     6901 agaccgactc cagaacttca tgggtgcatg ggcgaggccc gacgtcgtcg ggctcctcga
     6961 ttccggccta tccggctaac tgcgttttaa caaccgaccg ggcgttcccg gccacaataa
     7021 taagagtgga ctggccacga acacggacga ataggtgccg aagatgacac cccagagcaa
     7081 ggcgagcgaa aaatcgtgaa gcacttcacc acccgccaca gtcagaggaa tcagcaccag
     7141 gacaaccgtc aaacttgtca cgatcgtccg gctcaggact tgattgacgg cattattgat
     7201 catcgtttct tcactatctc gtctccgtgt tcgcagattc tcacgaatgc ggtcgaagac
     7261 gacgaccgtg tccgtcagcg agtaaccggc caaggtcaga agcgcggtga cgacgagcaa
     7321 ggtgatttcc ttatccagaa gataaaaggc ccccaagacg gccaagacat cgtgaaacgt
     7381 ggcaagcgct gcggcaatgc caaatcgaaa ttcgaaccgt gccgcgatat agaggatgat
     7441 tccggcaaac gaaatgacga ccgcgatcag agcatcttcc tgaagtttct tgccgatcgt
     7501 tgggccgatt tcggtactgg actcgaccac gaaatgattg gagggaatct ccttcgtgaa
     7561 gacgcccacc acccgctcgg ccaccttttc ttcaatcgtg gtggatgcct tcacgcgaat
     7621 cagcagtttg ttttcttgcc cgaattcctg cagctcggca tcccccaacc catttgcttc
     7681 cagcgccttc ctggccacat cgatatggat cggctgatcg aacttcagct ggaccgccgt
     7741 ccctcctgcg aaatcgatcc ccagattcgc tgcaccacga gcaatctgca ccacggcaat
     7801 gattcccagc agcaccatga cgccggaaaa tgtaaaggcc agatggcgct tgctcatgaa
     7861 gtcgaaattg gtcttcccaa gaatttctaa catgcaggct ccttcgaact aaatgcttaa
     7921 ctgttccact ttttgtcgtt gattcacgat atcgaaaata actttcgtgc cgaccaacgc
     7981 ggtaaagagg ttaatagcaa taccgaggca cagggtcaca gcgaatccct tgatcgggcc
     8041 ggtcccaaac agaaagagcg ccacgcccgt gatcaaagtg gtcacgtggg agtccacgat
     8101 tgtgagcaac gctttctcat agcccccatc caccgccagc cgcactggtt ttccctgccg
     8161 cagttcctcc ctgatacgct caaagatcaa gacgttcgaa tcgacgccca tcccgatggt
     8221 gagcacaatc ccggcgatgc cgggcaatgt cagcgttgcg ttcaaggcag agagggctcc
     8281 gatcaaacag acgaggttca gaatcaacgc gaaatccgcg accacgccgg agagtttgta
     8341 atagacgatc ataaagacga cgaccattgc gcccgcgatg agggtcgcct gcaccccctt
     8401 gtcgatggag tcttgcccca gcgagggtcc caccgtgaga tcttggatga tcctcaacgg
     8461 agccggtaat gccccagccc gcagcacgat ggctagatcg ttggcttcct gcgtcgaaaa
     8521 tgtccccgta atctgcgcgc gacctccggt aatacgttct tgaatcaccg gcgcggagta
     8581 gatcgagttg tcgagaacaa tcgccatccg ctttttgaca ttctctccgg tgatccggtc
     8641 aaactccttt ccgcctttgg cgtcaaacgt aatggacaca tacggatcgt tgaattggcc
     8701 gattgccacc cgtgcatcgc tcaggacgtc tccggtcaac atgacccgct ttttgacgac
     8761 gtaggggatg cgatattcac ggcctgtatc tttctcaacc actcgctcga agaggatttg
     8821 atcgccctca ggaagcttgg cctcagcctg cttcagcacg tcagcttccc tgtccttcgg
     8881 cacgcgagcc ggaagatcga gcttgagctg gttgtcctcg tcaagcatct tgaactccag
     8941 caatgccgtt tccttgatga ggtccttcgc ccgcttcgga tctttgacgc cggggagctg
     9001 caccacgatt tgcttgagcc cttgccgttg gaccaatggt tcggctacgc cgaactggtc
     9061 gattcgattt cggatcgtct ccagcgcttg gttgatcgca gagtctttaa tccgcttaat
     9121 ttccgtatcc cgaagatccc atatcaaggt atcgcttgat cccgctgact ccgcttcggt
     9181 aaacgtggga tagtcgtcga tcaatttctt aatctgatct ttcagttccg ggttctgaaa
     9241 ggtgatggtg atccggttta acccggtccg cttgaccgac tcgacaggaa tcttcttgtc
     9301 aaccaacaga tcttggagcg agaccaccgt ccgatcgagg gcgatttcga ccgctcgatc
     9361 ttcgtcgacc tccatcacca ggtgaatgcc cccctgcaaa tctagtccca gcgtgatgcc
     9421 tttgtccggc aacagcttac gagcccaatc cggaagggcc tgaaaaagcg gcctatacgt
     9481 cggcacaaag aaaacgagcg acacgataat gaccaagctc agcagcagta accgcccacc
     9541 cactctcttc atgttatttt caaccccttc ttagcttgca tactacgatt ctttgtcgtc
     9601 ttcatctccg cgaatacgcg caatatgttc ccgctgaatt ttgattttcg tgttgtcggc
     9661 gatctgcagc gtcacggtgt ctttgcccag attggcgata gtcccccaga tgccggaggc
     9721 cgtgacgatt ttgtcgcctt tcttcaatgc ctccatcatc gtcttctgct gtttctgccg
     9781 cttctgctgc ggcaggatga gcaagaaata aaagatgacg aagatcaaga cgaagggaac
     9841 gagcgacaat aacgatccca tcccccctcc gcccccggtt gatccttgag cccatgcgac
     9901 ggattgcatc accatgtcgg ttttttccct cccggctcga ttaatcctgt ctcgttcgtc
     9961 gactgtcgtc cacatcgcct gtcgaattca gcacctcatc ggtttccatc tctccacgac
    10021 tccggtagaa ctcggctcga aacgcgctga aagtcccttg ctcaatcgca acccggatgc
    10081 ggcgcatgag atcggcgaaa taccagaggt tgtgaatcgt attgagcctt gagccgagca
    10141 tttctttcac gttgaa
//
LOCUS       DATDNW010000160         2904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_17148,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000160 DATDNW010000000
VERSION     DATDNW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17148"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VI359_03910"
     CDS             <1..925
                     /locus_tag="VI359_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6973432.1"
                     /translation="SCWYYLRFIDPKNAQRLVDPEKERYWMPVDLYIGGSEHAVLHLL
                     YARFWHKVLFDIGVVSTPEPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMMDQFGAD
                     AVRLYEMFMGPLEAMKPWSTRGVEGVTRFLERVWRLMVNEEGALSSAVVSVAPSLDQQ
                     RLLHQTIKKVTEDIETLRFNTAISQMMVFTNELTKIERRPRALLEPFVLVLSPFAPHL
                     AEELWEKLGGRTSVSQQPWPAFDPALTISDRLTIPIQVNGKLRGKIEVLVDTSGAEVE
                     SLARAQIAEWMQGEEPKKVIYVEKKLINFVV"
     gene            1045..1614
                     /gene="lptE"
                     /locus_tag="VI359_03915"
     CDS             1045..1614
                     /gene="lptE"
                     /locus_tag="VI359_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY6973433.1"
                     /translation="MATALGACGYQFRVEGAGPTIGGTSVPASSEPPPRLVIRTLENK
                     SFEPNLETRYTNYLRHEFSSGSGAQIVPESEAADLVLTGQILSVIVPTLSFTQTTTLE
                     SRTEVIVLVKIEETRTKKVVWMQTAKGSSEFFVTTDLQFNRALQNRALEQAGRFIAAD
                     LASRFLLQLESGKLSKSTTDPTATTTGSK"
     gene            1632..2744
                     /gene="holA"
                     /locus_tag="VI359_03920"
     CDS             1632..2744
                     /gene="holA"
                     /locus_tag="VI359_03920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY6973434.1"
                     /translation="MPSTVTQTQLQASLKQGVLAPLYLVVGEDDLLRDTALSTLKVAV
                     LGEGGDTFNCDVFYGDEASGADIVACASEVAVFAPRRLVVVKGADKLPARECEVVLPY
                     LQVPNDTTTVLFIAPKMDGRLKFTQALARVAVSVDCSTPSDAQLTPWLKQDAERVGVR
                     LSDDALHMLKEACGGSLYSARRELEKLAAYVPSERAATASDVEALRGIEPGASVFDLA
                     MAIGTRDRGRVLSILARNLDAGEPPLRMLGSLAWQYRRLWKAKDLLDQGGRESEAARM
                     LRMDPYRVRAYVGQFSESHLSEALRRFLEVDAKLKGGSGGKPARIFEQLLLRLCDRAQ
                     GERRASSPDAPSSRETTRTKPLSNIRTVTSGKRSKN"
     gene            complement(2716..>2904)
                     /gene="rpsT"
                     /locus_tag="VI359_03925"
     CDS             complement(2716..>2904)
                     /gene="rpsT"
                     /locus_tag="VI359_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY6973435.1"
                     /translation="GLVKKLQTAVAAKKIDEAKASLRDATSALSKAVTKGVLKANTAS
                     RRISRLTLRVNSLTASRS"
BASE COUNT          643 a          731 c          885 g          645 t
ORIGIN      
        1 gtcctgctgg tattacctcc gatttatcga tccgaagaat gcgcagcgcc ttgtcgatcc
       61 agagaaggaa cggtactgga tgccggtgga tctttacatc ggcggcagtg aacatgccgt
      121 attgcatttg ttgtacgcaa ggttttggca caaggtactc tttgacatcg gtgtcgtgag
      181 cacgccggag ccgttcaaga aacttgtcca tcaaggcatc gtgcttgggg aagacaatca
      241 aaagatgtcg aaatcgcgtg gcaacgtggt caatcccgac gagatgatgg atcagttcgg
      301 ggctgatgcg gtgcgcctct atgaaatgtt tatgggtccg ttggaggcga tgaagccctg
      361 gagtacgaga ggggtcgaag gagtcacgcg gtttcttgag cgggtctggc ggctgatggt
      421 gaacgaggag ggcgccctgt caagcgctgt cgtgtctgtg gcaccgagtc tcgatcaaca
      481 gcgccttctg catcaaacga tcaaaaaggt gacggaagac atcgaaacac tccgattcaa
      541 cacagccatt tcacagatga tggtgtttac caacgagctg acgaaaatcg agcggcggcc
      601 ccgggcgctg ttggaaccgt tcgtattggt cctctcgccg tttgctccac acctggccga
      661 agaactttgg gaaaaattgg gtggtcggac cagtgtttca caacagcctt ggcctgcatt
      721 cgatcccgct ttgacgatca gcgaccggtt gacgattccc atccaggtga acggaaagct
      781 tcgcggaaag attgaggtcc tggtcgacac gtccggagca gaggttgaaa gcctcgcgcg
      841 agcgcagatc gccgaatgga tgcagggcga agagccgaag aaggtcattt acgtcgagaa
      901 gaagctcatt aactttgtgg tctgaaaagt gctgaggacg gggtgctgaa actcaagact
      961 gacaagaacg cgattccaca taaagggctg cataggcact ctgttctccg tactcggtac
     1021 tcagtccttg gtgttgccgt cgcaatggct accgcgctcg gcgcgtgcgg ctaccagttt
     1081 cgcgtcgaag gcgccgggcc tacaatcggt ggaacatccg ttcctgcctc gtcagagcca
     1141 ccaccgcgac tggtgatcag gacgttggag aataaaagtt tcgaacccaa tctggaaacg
     1201 cgttatacga attatttgcg ccatgaattt tcatcgggga gcggtgccca gattgtcccg
     1261 gagtcggaag cagcggattt ggtgctcaca ggccaaatcc tgtcggtcat cgttccaacg
     1321 ttgagtttta ctcagaccac gactctcgaa agccggacgg aggtcatcgt cctcgtgaaa
     1381 attgaggaga cgcgtacgaa gaaagtggtg tggatgcaga cggccaaggg ctcatcggaa
     1441 ttttttgtga cgacggacct tcaattcaat cgcgctctgc aaaatcgcgc cctggagcaa
     1501 gcgggacggt tcattgccgc agatttggcg tcgcgattcc tgttgcaact ggagtcggga
     1561 aaactgtcga agtcgacgac ggatccgact gcgactacga caggaagcaa gtagaaagaa
     1621 cgggtcaaca aatgccctcg accgttacac agactcaatt gcaggcctcg ctcaagcagg
     1681 gcgtacttgc tccgttgtat ctcgtcgttg gcgaagacga tcttctacgt gatacagcgt
     1741 tatccacact taaggtcgca gtgctcggag aaggtggaga tactttcaat tgcgatgttt
     1801 tttacggaga tgaagccagc ggagcggaca ttgtggcctg tgcttcggaa gtggcggtat
     1861 ttgcacctcg acgtctcgtt gtggtgaagg gggctgataa attgccagcg cgagaatgtg
     1921 aggtggtgtt gccatacctt caggtgccta atgacacgac gacagtcctg ttcatcgcgc
     1981 ctaagatgga tggtcgcctg aagtttacac aagcgcttgc gcgcgttgcc gtttcagtcg
     2041 attgttcgac tccgtcggat gcacagttga cgccgtggct caaacaggat gccgagcgcg
     2101 tgggtgttcg actcagcgat gacgcgttgc acatgctcaa ggaagcctgt ggcgggtcgc
     2161 tctactcggc gcgtcgagag ttggagaagc tagcagcata tgttccgtcc gaacgagccg
     2221 cgacggccag cgatgtagag gcgttacgcg gaatcgaacc cggtgcatcg gtgttcgatt
     2281 tggcgatggc gatcgggaca cgtgatcgcg ggcgcgtctt gtcgatcctg gcccgtaatc
     2341 tcgatgcggg cgagcccccc ctgcgaatgt tgggctcgct cgcgtggcaa tatcgccgtc
     2401 tctggaaagc caaagatctc ctcgatcaag gtgggcgtga atccgaagcc gctcgaatgt
     2461 tgcggatgga tccttatagg gtgcgagcct atgtggggca gttttccgag tcccacttga
     2521 gtgaggcgct cagacggttt ctggaggtcg atgcaaaact caagggaggg agcggaggga
     2581 aaccagctcg aatcttcgag cagcttttgc tgcgcctctg cgatcgtgcc cagggcgagc
     2641 ggcgcgcctc atcaccggat gctccttcgt cgagagagac aacacggacg aagccattgt
     2701 ccaatatccg gacggttacg agcgggaagc ggtcaaagaa ttgacccgaa gagtgaggcg
     2761 agaaatccga cgagatgctg tgtttgcctt aagcacgcct ttcgtcacgg ccttactcag
     2821 cgccgatgtc gcatcgcgca atgaagcctt ggcctcatca atcttttttg cggccacggc
     2881 cgtttggagt ttttttacta gacc
//
LOCUS       DATDNW010000161         4742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_776353, whole genome shotgun sequence.
ACCESSION   DATDNW010000161 DATDNW010000000
VERSION     DATDNW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776353"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VI359_03930"
     CDS             <1..925
                     /locus_tag="VI359_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6973436.1"
                     /translation="SCWYYLRFIDPKNAQRLVDPEKERYWMPVDLYIGGSEHAVLHLL
                     YARFWHKVLFDIGVASTPEPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMMDQFGAD
                     AVRLYEMFMGPLEAMKPWSTRGVEGVTRFLERVWRLMVNEEGALSSAVVSAAPSLEQQ
                     RLLHQTIKKVTEDIEALRFNTAISQMMVFTNELTKIERRPRALLEPFVLVLSPFAPHL
                     AEELWEKLGGRTSVSQQPWSAFDPALTISDRLTIPIQVNGKLRGKIEVGVDVSREEVD
                     RLARAQITEWVQNKEPKKVIYVEKKLINFVV"
     gene            959..1591
                     /gene="lptE"
                     /locus_tag="VI359_03935"
     CDS             959..1591
                     /gene="lptE"
                     /locus_tag="VI359_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY6973437.1"
                     /translation="MGEWWKLRQDRVRAAYFACVACLALLSACGYQFRVEGAGPTIGG
                     ASVPASSEPPPRLVIRTLENKSFEPNLETRYTNYLRHEFSSGSGAQIVPESEAADLVL
                     TGQILSVIVPTLSFTQTTTLESRTEVIVLVKVEETRTKKVVWVQTAKGSSEFFVTTDL
                     QFNRALQNRALEQAGRFIAEDLASRFLLQLESRKLSKSTTDPTATTTGSK"
     gene            1609..2721
                     /gene="holA"
                     /locus_tag="VI359_03940"
     CDS             1609..2721
                     /gene="holA"
                     /locus_tag="VI359_03940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY6973438.1"
                     /translation="MPSTVTQTQLQASLKQGVLAPLYLVVGEDDLLRDTALSALKVAV
                     LGEGGDTFNCDVFYGDEASGSDIVACASEVAVFAPRRLVVVKGADKLPARECEALLPY
                     LQMPNDTTTVLFIAPKMDGRLKFTQALARVAVSVDCSTPSDAQLTPWLKQDAERVGVR
                     LSDDALHMLKEACGGSLYSVRRELEKLAAYVPSERAATVNDVEALRGIEPGASVFDLA
                     MAIGERNRGRVLSILARNLEAGEAPLRMLGSLAWQYRRLWKVKDLLEQGGRESEAARM
                     LRMDPYRIRAYVGQFSESHLSDALRRFLEVDAKLKGGSGGKPARIFEQLLLRLCDRAQ
                     GERRASSPGAPSSGETTRTKPLSNIRTVTSGKRSKN"
     gene            complement(2693..2971)
                     /gene="rpsT"
                     /locus_tag="VI359_03945"
     CDS             complement(2693..2971)
                     /gene="rpsT"
                     /locus_tag="VI359_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY6973439.1"
                     /translation="MPVVHKSTIRRARQSEKRHERNHATLSAVKGLVKKLQTAVAAKK
                     IDEAKASLRDATSALSKAVTKGVLKANTASRRISRLTLRVNSLTASRS"
     gene            3060..3773
                     /gene="pyrF"
                     /locus_tag="VI359_03950"
     CDS             3060..3773
                     /gene="pyrF"
                     /locus_tag="VI359_03950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY6973440.1"
                     /translation="MSKIAARDRLILALDVPSSAEADRLLDHVHDQICFVKIGLELYT
                     AAGPDMVKRVIDRGKRVFLDLKFLDIEETVRRATARVAAMGVELLTVHANRKALAAAV
                     QGRGNSSLKLLAVTVLTNFDSQDLRDMGIQHSVQELVSARALLASEVGCDGVVASGEE
                     AGVLRPKVGPRFLIVTPGVRPAGKGVDDHARVTTPTQTIVAGADYLVIGRPIRDAADP
                     AAAAAAILSEMQAAFDARG"
     gene            3857..3931
                     /locus_tag="VI359_03955"
     tRNA            3857..3931
                     /locus_tag="VI359_03955"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3889..3891,aa:His,seq:gtg)
     gene            4479..>4742
                     /locus_tag="VI359_03960"
     CDS             4479..>4742
                     /locus_tag="VI359_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6973441.1"
                     /translation="MCKQLKGHKGFTLIELMIVVAIIGILASIAIPNFLAYQARSRQA
                     EARTDLGAVFVSETAFFGENNRFSDFATVGYSPTATSNRYTYRS"
BASE COUNT         1033 a         1202 c         1431 g         1076 t
ORIGIN      
        1 gtcctgctgg tattacctcc gatttatcga tccgaagaat gcgcagcgcc ttgtcgatcc
       61 agagaaggaa cggtactgga tgccggtgga tctttacatc ggcggcagtg aacatgccgt
      121 attgcatttg ttgtacgcaa gattttggca caaggtactc ttcgacatcg gtgtcgcgag
      181 cacgccggaa ccatttaaga aacttgtcca tcaaggcatc gtgctcgggg aagacaatca
      241 aaagatgtcg aaatctcgcg gcaatgtcgt gaatcccgac gagatgatgg atcagttcgg
      301 ggctgatgcg gtgcgcctct atgaaatgtt catgggcccg ttggaggcga tgaagccgtg
      361 gagtacgaga ggagtcgaag gagtcacgcg gtttttggag cgggtctggc ggctgatggt
      421 gaacgaggag ggcgccctgt caagcgctgt cgtgtctgcg gcaccgagtc tcgagcaaca
      481 gcgccttctg catcaaacga tcaaaaaggt gacggaagac atcgaagcac tccgattcaa
      541 cacagccatt tcacagatga tggtgtttac caacgagctg acgaaaatcg agcggcggcc
      601 acgggcgctg ttggaaccgt tcgtattggt cctctcgccg tttgctccac acctggctga
      661 agaactttgg gaaaagttgg gtggtcggac cagtgtttca caacagcctt ggtctgcatt
      721 cgatcccgct ttgacgatca gcgaccggct cacgattccg attcaagtga acgggaaact
      781 acgcggaaag atcgaggttg gggtagacgt gtctcgagaa gaagtcgacc ggcttgcgcg
      841 agcgcagatc accgagtggg tgcagaacaa agagcccaag aaagtcatct acgttgagaa
      901 gaaactgatc aacttcgtgg tgtagtagct gatggataca agacgaaata ggcacaacat
      961 gggagaatgg tggaaactac gacaagatcg tgtgcgtgct gcctatttcg cgtgcgtcgc
     1021 ctgccttgcg cttttgagcg catgcggcta ccagtttcgc gtcgaagggg ccgggcctac
     1081 aatcggcgga gcatccgttc ctgcctcgtc agagccaccg ccgcgactgg tgatcagaac
     1141 gttggagaat aagagtttcg aacccaatct ggaaacgcgt tatacgaatt atttgcgtca
     1201 tgagttttca tcggggagcg gtgcccagat tgtccccgag tcggaagctg cggatttggt
     1261 cctcacaggc caaatcctgt cggtcatcgt tccaacgttg agttttactc agaccacgac
     1321 tctggaaagc cggacggagg tcatcgtcct cgtgaaagtt gaggagacgc gtacgaagaa
     1381 agtggtgtgg gtgcagacgg ccaagggctc atcggaattt tttgtgacga cggaccttca
     1441 attcaatcgc gccctgcaaa atcgtgccct ggagcaagcg ggacggttca tcgccgaaga
     1501 tttggcgtcg cgattcctgt tgcaactgga gtcgagaaaa ctgtcgaagt cgacgacgga
     1561 tccgactgcg actacgacgg gaagcaagta gaaagaacgg gtcaacaaat gccctcaacc
     1621 gttacacaga ctcaattgca ggcctcgctt aagcagggcg tacttgctcc gttgtatctc
     1681 gtcgttggcg aagacgatct tctgcgtgat acagcgttgt ccgcactcaa ggtcgcagtg
     1741 ctcggagaag gtggagatac tttcaattgc gatgttttct acggggatga agccagcgga
     1801 tcggacattg tggcctgtgc ttcggaagtg gcggtatttg cacctagacg tctcgttgtg
     1861 gtgaaggggg ctgataaatt gcccgcgagg gaatgcgagg cgttgttgcc ataccttcag
     1921 atgcccaatg acacgacgac agtcctgttc atcgcaccca agatggatgg tcgcctgaag
     1981 tttacacaag cgcttgcgcg cgttgccgtt tcagtcgatt gttcgactcc gtcggatgca
     2041 cagttgactc cgtggctcaa acaggatgcc gagcgcgtgg gggttcgact cagcgatgac
     2101 gcgttgcaca tgctcaagga ggcctgtggg ggatcgctct actcggtgcg tcgagagttg
     2161 gagaagctgg cagcatatgt tccgtccgaa cgggccgcga cggtcaacga tgtggaggcg
     2221 ttacgcggaa tcgaacctgg tgcatcggtg ttcgatttgg cgatggcgat cggggagcgt
     2281 aatcgcgggc gcgtcttgtc aatcctggcc cgtaatctcg aggcgggcga ggcccccctg
     2341 cgaatgttgg gctcgctcgc gtggcaatat cgccgtctct ggaaagtcaa agatctcctc
     2401 gaacaaggtg ggcgtgagtc cgaagcggct cggatgttgc ggatggaccc ttataggata
     2461 cgagcctacg tggggcagtt ttccgagtcc cacttgagtg acgcgctcag acggtttctg
     2521 gaggtcgatg caaaactcaa gggagggagc ggaggaaaac cggctcgaat cttcgagcag
     2581 cttttgctgc ggctctgcga tcgtgcccag ggcgagcggc gcgcctcatc accgggtgcc
     2641 ccttcgtcgg gagagacaac acgcacgaag ccattgtcca atatccggac ggttacgagc
     2701 gggaagcggt caaagaattg actcgaagag tgaggcgaga aatccgacga gatgctgtgt
     2761 ttgccttaag cacgcctttc gtcacggcct tactcagcgc cgatgtcgca tcgcgcaatg
     2821 aagccttggc ctcatcaatc ttttttgccg ccacggccgt ttgaagtttt tttacgagac
     2881 cctttacagc gctcaaggtg gcgtgattgc gctcatgacg tttttccgac tggcgagccc
     2941 ggcgaatcgt cgacttgtga acaacaggca ttgcatgctc ctatacaaaa gaggagcgag
     3001 tttgtaccat agggccgatc gaggcgtcaa gatgagcctc cttcagagag gaatcatcag
     3061 tgtcgaaaat cgccgcacgt gaccgtctga ttctagccct cgacgtgccg tccagtgccg
     3121 aggcagaccg gttgttggat cacgtccatg accagatctg ttttgtcaaa attgggctag
     3181 aactctacac agctgcgggt cccgacatgg tgaagcgcgt gatcgatcgt gggaaacgtg
     3241 tctttttgga tctcaagttt ctggacatcg aggaaaccgt tcgccgcgct accgcacgcg
     3301 tcgccgctat gggagtggag ttgctgacgg tgcacgcgaa tcgcaaagcc ctcgcggcag
     3361 ccgtgcaagg ccgtggaaac tcaagtctca aacttcttgc agtgacagtg cttacgaatt
     3421 ttgatagtca agacttgcgt gacatgggca ttcagcacag cgtgcaggaa ctggtcagcg
     3481 cccgggcgtt actggcctca gaggttggat gcgacggagt tgtggcgtcg ggggaagaag
     3541 ccggtgtgct tcgccccaaa gtcggtcctc gcttcttgat cgtcacgcca ggagtccgtc
     3601 cggccgggaa gggtgtggat gaccatgcac gtgtgacgac gccgacccaa acaatcgtgg
     3661 ccggggcaga ctatctggtc atcggacgtc ccatcaggga tgcggcagat cctgccgctg
     3721 cggcagccgc cattctttcc gagatgcaag cggctttcga cgcgcggggc tgattaccca
     3781 gccgtggttg cgagccttct ctgctctttg ctaagattct tttcgcgagt cgtctcgttc
     3841 ccggcttctc tcagtggtgg gtgtagctca agggtagagc gcctgactgt ggctcaggag
     3901 gctgcgggtt cgaagcccgt cactcacccc aattcccttc ttcgtgactt ccggattgct
     3961 caattgacct gaggccgtaa tagtcatcac cgtacttctt tggttgcgcc caggataatg
     4021 cctgcggttc agtcatcaac tcagccggtg tcgcccgatt gtagatcgtt ggatcttctt
     4081 aggcgaaatg ggccttctcc tactccgcca ggcaagtcct gccacggaaa aattcaggaa
     4141 ctgggaaata aagggctgaa gtaagccgat gccatctttg aaatggataa gtttgtcaaa
     4201 atggacgtca tcgggtgcca atttttggca gcttcgagca agaaactgaa tgatcagtcc
     4261 ggataatagc tgagaattgt ggacaggccg ctttctaaca cattgattta ccgtttattt
     4321 ttctatctga cggcgtccag gtttccccgg catcgaacct gctgatacgt atctcctgct
     4381 attggggaga ggcctatgcg ggcgcctagg gggtttccag agcggacaac ggagcgagca
     4441 agggcctgtc agcgaaccat catcaaggaa gagcagtaat gtgtaagcag ttgaaggggc
     4501 acaagggttt tacgttgatc gaattgatga tcgtggtggc gatcatcggg atcttggcat
     4561 cgatcgccat cccgaacttc ttggcgtacc aggcccggtc tcgccaggct gaagctagga
     4621 ccgacctggg tgcggtgttt gtgtcagaaa ccgccttctt cggcgaaaat aatcggttct
     4681 cggattttgc aacggttggt tattctccta ccgcgacttc aaaccggtac acctaccgga
     4741 gt
//
LOCUS       DATDNW010000162         3912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_158345, whole genome shotgun sequence.
ACCESSION   DATDNW010000162 DATDNW010000000
VERSION     DATDNW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158345"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VI359_03965"
     CDS             complement(<1..297)
                     /locus_tag="VI359_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973442.1"
                     /translation="MKKVRVLLADEHPPLLHHLSALLSEDFYVVGTVSDGKALVTEAL
                     KLEPQVIISDIDMPIMGGLEAVRQVKALMPDIKIIFLTNHKEQEYVAAAFSAGAS"
     gene            complement(467..1111)
                     /locus_tag="VI359_03970"
     CDS             complement(467..1111)
                     /locus_tag="VI359_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973443.1"
                     /translation="MNKPRVLLADDHTLVLDGFRKLLEDHCEIVGAVEDGRALLDLAE
                     RCQPDVITLDISMPQLNGIDAARRLRKIAPQAKLIFVTMHADQAYISEAFKSGASGYL
                     LKRSAGSELVQAIHTVMNGNYYVTSLIAKDLVHSALAGGRAPVIQRDTLTVRQREVLQ
                     LVAEGRTVKEIAGVLNISPKTVEFHKSQLMEQLDLHTTAELTKYALTHGLISSE"
     gene            complement(1108..2226)
                     /locus_tag="VI359_03975"
     CDS             complement(1108..2226)
                     /locus_tag="VI359_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6973444.1"
                     /translation="MTHIQARQGLITGVVAALTVGIFVFDILTPRGLTNQVLYLVPLL
                     ISFLSTGSTFPLAVASACSVLTIIGLFLSPDTFNIPLWVILSNRLFSLVIIWTPVLFF
                     QQRRRHEEQLTRMNEELEQRVQDRTKDLASVNQALVAEVSERMHTERMLDASRHELRS
                     LAAELLRVQEEERRRISRDLHDDINQRLALLAVELEALDRPLPMTPQERGRVVRAVQD
                     RVAELSEDVRRLAYQLHPSILDDLGLPIALQRLVDDFTTRTGMSGQFVNRGVSKALPQ
                     DVATCLYRVAQESLRNIARHARASTMTVELSRKQDMLTIVITDDGVGFDPDLVRSGLG
                     LLSMKERIALVSGSLDIASTPGKGTHVCVRVPQVGDNP"
     gene            2858..3541
                     /locus_tag="VI359_03980"
     CDS             2858..3541
                     /locus_tag="VI359_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6973445.1"
                     /translation="MATPTVIFLFDVDNTVLDNDRVTADLRQHLEREVGTQREQLYWT
                     IFEQLRAELGYADYLGALQRYRVEYPRDPHVLTVSRFLVNYPFANRLFPHALDVVDHV
                     KQWGRAVILSDGDVVFQPRKIERSGLFAAVDGNVLIYIHKEQELDDVERRYPADHYVL
                     VDDKLRILTAVKKGWGNRVTTVFPRQGHYARDPQAMTSYPAPDLAIEQIGDLLKYDLH
                     ALLALAPST"
BASE COUNT          856 a         1062 c         1115 g          879 t
ORIGIN      
        1 cgaggcgcct gcagaaaacg ccgctgcgac gtattcctgc tctttatgat tggtcaggaa
       61 aataatcttg atgtcgggca tgagcgcttt cacttgtcgg acggcttcca gtccgcccat
      121 gatcggcata tcgatatcgg aaataatcac ctgcggttct agtttcaacg cttccgtcac
      181 gagggctttt ccgtcgctca ctgttccaac gacatagaaa tcctctgaga gcaacgcact
      241 caggtggtga aggagcggtg ggtgttcatc ggcgagcagc acacgcactt tcttcatacc
      301 aatcctcctt gacacagtgc atgaacagcc aacgggtgtc gcccaggtat tgctatatgt
      361 gtactgaatc gcaactagga gcagaactag ggatatacct aggacgagcg agaaagacta
      421 ccgggtaggg gaacacgggg cgaggtgatg ttcggtggac acgctgttat tcagacgaga
      481 taagtccgtg ggtcaatgca tatttcgtca attcggcggt ggtgtgaaga tcgagttgtt
      541 ccatgagctg tgatttgtgg aactcgactg ttttggggga gatattaagg acgccggcga
      601 tttccttgac cgtccggccc tcggcaacca gctgtaagac ctcgcgctga cggacggtga
      661 gcgtgtcccg ctggatcaca ggggctcggc cgcccgcgag agcggagtgg accaggtcct
      721 tggcgatcag ggatgtcaca taatagtttc cgttcatcac cgtgtgaatc gcctgaacaa
      781 gctcggagcc ggcggaccgc ttgaggagat agcctgaggc tccggactta aaggcttcgc
      841 tgatataggc ctgatctgca tgcatcgtga caaagatcag tttggcctgt ggggcgatct
      901 tgcggagtcg cctggccgca tcgatgccgt tgagctgggg catcgagata tccagcgtaa
      961 tgacgtccgg ttggcaccgt tccgcaaggt cgagcagcgc ccgtccgtct tccacggcac
     1021 cgacgatctc gcaatggtcc tcaagcagtt tacgaaatcc gtctaggacc agcgtgtgat
     1081 catcggcgag cagcacgcgc ggtttgttca tggattgtct ccgacttgtg gcacgcggac
     1141 gcacacatgc gtccctttgc caggcgtgga agcgatgtcc agtgatccgc tcaccagtgc
     1201 gatccgctcc ttcatgctca gcaaccccaa cccgcttcgc acgagatcgg gatcgaaccc
     1261 gaccccgtcg tcggtgatca cgatcgtgag catgtcctgt ttgcgactga gttcgacggt
     1321 cattgtcgat gcccgcgcgt gccgggcgat gtttctcaaa ctttcctgtg cgacacgata
     1381 caaacatgtg gcgacatcct gtggcaatgc ctttgagaca ccacggttca caaattgccc
     1441 gctcattccg gtccgagtgg tgaagtcgtc cactaaccgt tggagtgcga tgggaaggcc
     1501 gaggtcgtct aggatggaag ggtgtaattg gtaagccaga cgccgaacat cctcggaaag
     1561 ctccgccacc cggtcttgca cggcccggac gacacgacca cgctcttgcg gcgtcatcgg
     1621 cagcggtcga tccagggctt ccagctccac ggcgaggagg gctaatcgtt gattgatgtc
     1681 gtcgtgaagg tcgcgggaga tacgccggcg ttcctcctcc tgcacgcgca gcaactcggc
     1741 agccaacgat cgtaattcat ggcgactcgc gtccagcatt cgttctgtgt gcatccgttc
     1801 agaaacctcc gccaccagcg cctggttgac cgatgccaaa tccttggtac ggtcctgcac
     1861 acgctgttcc agttcttcgt tcatccgcgt gagttgttcc tcatgcctgc ggcgttgctg
     1921 gaagaacaac accggtgtcc atatgatcac caaactgaac agccggttgc tgaggatcac
     1981 ccagagaggg atattgaatg tatcaggact caaaaacaaa ccaatgatcg tgaggacgct
     2041 gcacgcgctt gccacggcga gaggaaaggt tgaccccgtc gaaagaaagg aaatcaggag
     2101 cgggaccagg tacaggactt ggttcgtcag accacgcggc gtgaggatgt caaacacaaa
     2161 gataccgacc gtcaacgcgg cgacgacacc ggtgataagc ccctgtcttg cctggatatg
     2221 cgtcacgcga actcccggtt gaattatcct ccctcgtagg gaggcggttc aagcggaggg
     2281 aaatcgctag tacatgagta tgaggcggac gatgatgaag cccgaggtgc cggaggcggt
     2341 ataggtcgaa ttggcgccct tgagatctgt atgttcccac atgctctgta tgcgaaattc
     2401 tcggttggcg taccaggtga gccccacgct gtaccctttt gcggtctggg catacagcgt
     2461 cgctcatgtg tcatttgaga accagtgaaa tgggctgctt ttaaaaacgg aaccacgcga
     2521 atttgatcgt tccagcacca ggttgcacgg tgcgaacaag aaagtacctg cgtgggtggt
     2581 cgcagcccct cgacaacgac ttcattattg gggaatactc ccgtctggag agctgggagc
     2641 aggaaaaccc gacatgccct ggttggttta tatagatcga tgaaccttgc tggttcgtat
     2701 cgtagcctca tggcatctca taggaaattc gagtgcgaga tgcatgacta gatctgtgag
     2761 tgatcagaaa aggcgtgacc ttcctgcgcc gtgtaaggaa gtagaggaac cgaagattcg
     2821 ccgttggcaa ttgaattgaa cctgctaggg gagtggcatg gcaacaccca cggtcatttt
     2881 cctcttcgat gtcgataaca cggtgctgga taacgatcgg gtcaccgccg atctgaggca
     2941 gcacttggag cgggaagtgg gtacgcagcg agagcaactc tattggacca tcttcgaaca
     3001 gctccgtgct gaactgggct atgcggatta tctgggggcg ctgcagcggt atcgtgtgga
     3061 atacccgcgc gatcctcacg tgctgaccgt ctcgcgattc ttggtgaact acccgtttgc
     3121 gaatcgcctg tttcctcatg cgctggacgt ggtcgaccat gtcaaacaat gggggagggc
     3181 ggtgatcctg tcggacggcg acgtggtctt tcaaccgcga aaaatcgaac gttccgggct
     3241 gttcgctgcc gtcgatggca atgtattgat ctacattcat aaagagcagg aactggatga
     3301 tgtcgagcga cgatatccgg ccgaccacta tgtgctggta gacgacaaat tacggattct
     3361 cactgcagtg aagaaaggct ggggtaaccg cgtgaccacc gtttttccgc gtcaaggtca
     3421 ctatgcgaga gatcctcagg cgatgaccag ctatcccgct ccagaccttg ccatcgagca
     3481 gatcggtgat ctgctcaaat acgatcttca tgcgctgctt gcgctcgcgc catccacata
     3541 acccaacgaa agccggcatc gcgttctctc tgcttctctc aatcggtaga aacgggcgtt
     3601 tctgcacaga ccacacaatt tatcaacaca aaccggatca gctgggaaaa gttgtttcca
     3661 tccgcacgag tctcaagcct cagtcacaat cttgcggcaa accgttgctc catcctcttg
     3721 gcaggaaatt gcttgattga cgggagcacg atgtcaatgt tctgcttggc tctttacgat
     3781 cgcttccgcc ttgagccaat cctccagatc gtggccgtct tcttgaccac gctgctcgta
     3841 cagattatag gcggtctccg caatgcgccg ccggataccg tcacccgatc cgttcgagct
     3901 ctctctatcg tt
//
LOCUS       DATDNW010000163         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_701347, whole genome shotgun sequence.
ACCESSION   DATDNW010000163 DATDNW010000000
VERSION     DATDNW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701347"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /gene="asnB"
                     /locus_tag="VI359_03985"
     CDS             complement(<1..1433)
                     /gene="asnB"
                     /locus_tag="VI359_03985"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY6973446.1"
                     /translation="MSKMNQALVHRGPDSQGITGDAHVTLAMRRLSIIDVLGGEQPLY
                     NETGTVAVVCNGEIYNYVELRDALIKKGHRFSSKSDVEVIVHLYEDMGMEFLTMLRGM
                     FAMALWDADKGRVVLVRDRMGEKPLYWYRDPRGRLWFSSEMKSLLQGIREYQPTLSLS
                     SVRLFLTYQYVPEPRTMFEGVFKLPAGHYLDVSTDRLDANPQPYWNYLAVPETDGNPI
                     HLVRHEIEAACRVMGRSDVPVGVALSGGVDSAAVAAVSAKSYPGTLQAFSVGYEGRPE
                     TDERAKAAEIADYLKIPFFEIELAINDVVRRLPDLAWAMDDPIADIAGFGYYAVSELA
                     RDHGVPVLLSGLGGDELFWGYPWIRDLVVEHERHRTSSSPAAWLRKMLASQSKRQQNL
                     VLSQLHEELRVADAWSRKLLASAPGARPLSDLAEESIQYAEDESLALPLSHLLNRTWL
                     VSNCLALADRVSMAHSVEMRLPLLDVRL"
     gene            complement(1571..>2504)
                     /locus_tag="VI359_03990"
     CDS             complement(1571..>2504)
                     /locus_tag="VI359_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY6973447.1"
                     /translation="YALSHSKQDGDMVLMMNNDTQVEPAYVETLVRVSMKENAAVGSL
                     IVDSQNPSRVLDAGEFINWQTYSFPVKTYIEPGNTFYDRVDVLPGRGTMVPLRMIRIA
                     GNVDDQVFPHYIADYEFFLRLRRHGFRLGVSSEVRILAHIEETGLFAEGFPVEIGQAW
                     ALVSSRKSMNNFRDHWRFIGHCAPPGYKVRSRIRLLWRTAHIFLLQTRLRYVVWPVVL
                     CWRWLRFLVYDNYYVTAADCARYGLDAKSLSAESILAPWRRSDWYKFARDRREWWGVR
                     PELHGLYRCAWNPSTKLRRWVKARGYRSGHGKAA"
BASE COUNT          590 a          764 c          621 g          529 t
ORIGIN      
        1 agcctgacgt ccaaaagcgg caaccgcatc tcgaccgaat gagccatact gacccgatcg
       61 gccagcgcga gacaatttga cacgagccac gtccgattca gcagatgcga cagcggcaag
      121 gcgagactct cgtcttcagc atattgaatc gattcttctg caaggtccga caatgggcga
      181 gccccaggtg ccgaagccag caacttccgc gaccatgcgt ctgcgactct caattcctca
      241 tggagctgac tcagcaccag gttttgctgc cgcttagatt gggacgcgag catctttcgc
      301 agccaagcag ccggactcga cgacgttctg tgacgttcgt gctccacgac aaggtcacga
      361 atccatgggt acccccaaaa gagttcgtcg ccacccaagc cggacagaag gacggggact
      421 ccatggtcac gcgccagttc ggagacggcg tagtacccga accccgcgat gtccgcgatg
      481 ggatcatcca tcgcccacgc cagatcgggc aagcgccgga cgacatcgtt tatggccaat
      541 tcgatttcga agaacggaat cttgagatag tccgcaattt ccgccgcctt ggcccgctcg
      601 tccgtctcgg gccttccttc ataccccacg ctaaaggcct ggagcgttcc cggataactt
      661 ttggcgctca ccgcggcaac agccgccgaa tccactcccc cactcaaagc aactccgacg
      721 ggaacatccg atcgacccat gacccggcaa gcagcttcga tctcatgtcg aacgaggtgg
      781 atcggattac cgtcggtctc cggcacagcc aagtaattcc aatagggttg aggattggca
      841 tcgagccggt cggttgacac atccagatag tgaccggcag gaagtttaaa cactccctcg
      901 aacatcgtcc gcggctcagg aacatactgg taggtcaaga atagccgaac cgatgataag
      961 gagagcgtag gctgatattc gcggatgccc tgcaagagac ttttcatctc gcttgagaac
     1021 caaagacgac ctctcggatc ccgataccaa tagagaggct tttctcccat tcgatctcgc
     1081 acaagcacca cacggccctt atcggcatcc cacaaggcca tggcaaacat gcctcgtaac
     1141 atcgtcagaa attccatccc catgtcttcg tagagatgaa caatgacttc gacatcgctc
     1201 ttggaagaga atcgatggcc ctttttgatc agcgcatctc ggagttcgac atagttgtag
     1261 atttcgccat tacacacgac cgcaaccgta cccgtctcgt tatacagtgg ctgttcccca
     1321 ccgagcacgt cgataatgct caatcgtctc atcgccaagg ttacgtgggc atcaccggtg
     1381 attccctgcg agtccggacc gcgatgaacc aaggcttgat tcatcttcga cacggtgtgt
     1441 cgttcactct cggtcagagg agtgagcgag attatcccgg ctataccgca catggcatta
     1501 cagtttcctt gtgatgtccc tcgagacgac cctgtcgatc gtgcatgccc tgccgagccg
     1561 gacgattggt ttatgctgcc ttcccatgtc cagatcgata ccctctggcc ttgacccatc
     1621 ggcgaagttt tgtggatgga ttccacgcac accgatacag cccatgtaac tctgggcgaa
     1681 ctccccacca ctctctgcgg tctcgagcaa acttgtacca atcacttctc cgccagggag
     1741 caagaatgct ctctgccgat aaggacttcg cgtcgagccc gtagcgcgcg caatccgctg
     1801 cagtgacata gtaattgtca tagacgagga acctcagcca ccgccagcac agcaccaccg
     1861 gccacacgac gtacctcaat cttgtttgaa gcagaaaaat gtgtgcggtt cgccaaagga
     1921 ggcgaattcg tgaacgaact ttgtagcctg gtggcgcaca atgaccgatg aaccgccagt
     1981 gatccctgaa attattcatg gattttctgg acgacacgag ggcccaagcc tgaccgattt
     2041 caaccggaaa tccttcagca aataacccgg tctcttcgat gtgcgccaga atacgtacct
     2101 cgctcgacac tcccaaccga aatccatgtc gccgcagccg gagaaagaac tcgtaatccg
     2161 cgatatagtg ggggaatacc tgatcgtcta cgttgcccgc aattcgaatc atacggagtg
     2221 gaaccatcgt tcctcgcccc ggcagtacat ccacccgatc gtagaaggtg ttccctggtt
     2281 cgatgtacgt tttcaccgga aacgaatagg tctgccagtt gatgaactcg cctgcgtcaa
     2341 gaacgcgaga cggattctgg ctatcgacga tcaacgaccc gaccgctgca ttttccttca
     2401 tagacacccg cacgagcgtt tcgacgtagg ccggctcgac ttgcgtgtca ttgttcatca
     2461 tcaagaccat gtcaccgtct tgcttgctgt gtgagagcgc atac
//
LOCUS       DATDNW010000164        14120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_840776, whole genome shotgun sequence.
ACCESSION   DATDNW010000164 DATDNW010000000
VERSION     DATDNW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 14120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14120
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840776"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VI359_03995"
     CDS             <1..343
                     /locus_tag="VI359_03995"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973448.1"
                     /translation="DIMIECRQYPRVPVDLQVFFSTTGNTDIREGTMFDLSLGGCAVT
                     TMTSVQPGTGIRILIRATDLGSPITIESAAVRWSHHGEFGVEFMGISDIDQSRLSRLL
                     QASTSYPIRPS"
     gene            672..1715
                     /locus_tag="VI359_04000"
     CDS             672..1715
                     /locus_tag="VI359_04000"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY6973449.1"
                     /translation="MISTKGYAALSAKEALQPFSFERRHVGAHDLLIKISHCGICHSD
                     IHQARNEWGGSIFPMVPGHEIVGVITQIGNAVKKFRVGDRAGVGCFVDSCRTCTACLE
                     GLEQYCEAGMLLTYSGRDQDGQPTQGGYSTQIVVDENYVLRLPSALSAAGTAPLLCAG
                     VTTYSPLRHWGVGKYHKLAVVGLGGLGHMAVKIARAMGTEVTVLSTSERKRMDAERLG
                     IAHFALTSQPETFTKLQRHFDFILDTVSAPHNYNAYVNLLKTDGTMILVGAPDKPTPL
                     EPFPLILHRRRIAGSVIGGIRETQEMLDFCADHHIESDVEVIPIQQVNEAYERVIRGD
                     VRFRFVIDLASLK"
     gene            complement(1750..1823)
                     /locus_tag="VI359_04005"
     tRNA            complement(1750..1823)
                     /locus_tag="VI359_04005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1789..1791),aa:Gln,seq:ttg)
     gene            complement(1878..1951)
                     /locus_tag="VI359_04010"
     tRNA            complement(1878..1951)
                     /locus_tag="VI359_04010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1917..1919),aa:Gln,seq:ctg)
     gene            complement(2026..3438)
                     /gene="gltX"
                     /locus_tag="VI359_04015"
     CDS             complement(2026..3438)
                     /gene="gltX"
                     /locus_tag="VI359_04015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY6973450.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLYARQQHGVFILRIED
                     TDQSRSTDESIQAIIEGMEWVGLDWDEGPYRQTDRMDLYRSHAMQLLEKGQAYWCVCK
                     AEELEARRKEAELKGLSPRYDGRCRNLGVINPTEEAALRFKAPQEGQTVVDDLIKGKV
                     VFDNTVLDDLIILRSNGYPTYNFSVVVDDGLMHITHVVRGDDHLTNTPRQVPIFQALG
                     FPVPQFGHLPMILGSDKARLSKRHGATSIMAYKEMGYLPEAMMNYLVRLGWSHGDQEL
                     FTRQELIEKFSWDHVQKSAAVFNPEKLIWMNAEYIKTSPPSQIAQRLLPLLEASGLQE
                     EVRTVSGGWLTQLVVLVRERTKTLTDMIMWVTPYFGQTVAFDEEAAKKFLTPAMAPVL
                     GKLVARFEALPTFSKQEWEASFKKLVEEEDMKMGQLAQPVRVALTGRTASPGLFEVME
                     VLGRERTLLRLRKGIERASA"
     gene            3560..4264
                     /locus_tag="VI359_04020"
     CDS             3560..4264
                     /locus_tag="VI359_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6973451.1"
                     /translation="MAELTQSATVLSVNDLTPQVRQLVLLPTVHNIAFQPGQWVSLKL
                     PVGAKPPLNRAYSMAAPGTPSGELTLVFDRVTGGLGTTYLYTLKPGSEVQFSGPHGNF
                     VLPKQLDRELILIARYTGLVPIRCMLKRLYAQRQTAPIMLIAVAPAEEELLFHQELLT
                     LAVTHPGFRYLPLVAAGGEQQGVDLTLSMLRPLIEGVPKVIPMLCGTKGFVRPLRAYF
                     VGMGYDRKEVKTETYD"
     gene            complement(4266..4685)
                     /locus_tag="VI359_04025"
     CDS             complement(4266..4685)
                     /locus_tag="VI359_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY6973452.1"
                     /translation="MGGTNPYIEKADYELPSKPYTITYIAPSGEVTKVEVDPGKIPYG
                     PTGLPGSVLDVAMANGVELEHVCGGVCACSTCHVIVKQGLESCNEGTDDEFDQLDEAP
                     MTTLQSRLACQCVPNGTKDIVVEIPAVNKNLVKEGHQ"
     gene            4821..5294
                     /locus_tag="VI359_04030"
     CDS             4821..5294
                     /locus_tag="VI359_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY6973453.1"
                     /translation="MLKISKKADYALMALQHIASVQFGDVTPGRVVNTKEIAEEYNIP
                     LELLAKVLQTLAKHGMIESHNGPKGGYLLARSARQLTIAQILESIEGPLGITDCSHEK
                     DGELCLQLEHCTIRTPLLKVQDSIYQLLNNMTLQDMMGGTPLITIQSPTTQGVER"
     gene            5291..6508
                     /locus_tag="VI359_04035"
     CDS             5291..6508
                     /locus_tag="VI359_04035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY6973454.1"
                     /translation="MKLPIFLDNHSTTPMDPRVLETMLPYFVEKFGNAASRNHAFGWE
                     AEEAVEQARKQIAKLIKADPKEIIFTSGATEADNLAIKGVLEMYREKGDHIITSSTEH
                     RAVLDTAKAMEAKGKATVTYLPVDKYGMVNPEDVRHAITEKTVLISIMLANNEIGTIN
                     PIKEIGKIAKEKGVLFHCDATQGVGKIPVDVQDMGIDLMSFTAHKIYGPKGVGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCGLEMATESVRLAAMRDR
                     LHVGITKVLEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKDIALSSGSACTSATL
                     EPSYVLRALGVGTELAHSSIRFGLGRFNTDDEIDYTIKKVIEVVTKLREMSPLYEMAK
                     EGVDLKSVQWAAH"
     gene            6537..6935
                     /gene="iscU"
                     /locus_tag="VI359_04040"
     CDS             6537..6935
                     /gene="iscU"
                     /locus_tag="VI359_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY6973455.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKDEEGVGTGMVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTVEEAQKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADGKTVDTK"
     gene            6955..7311
                     /locus_tag="VI359_04045"
     CDS             6955..7311
                     /locus_tag="VI359_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY6973456.1"
                     /translation="MDATNAETQAPVITLSEAALKEVKRLINVQGLTEGGLRLGVKGG
                     GCSGLSYTINFDDKIGQYDQVYEFDGVKVIVDVKSAIYLQGTQLDFQKDLMGGNFKFV
                     NPNANKTCGCGESFSA"
     gene            7389..8102
                     /gene="hscB"
                     /locus_tag="VI359_04050"
     CDS             7389..8102
                     /gene="hscB"
                     /locus_tag="VI359_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY6973457.1"
                     /translation="MDPHTHMTSRRELQMARSMCWHCQSEVAGEYFCERCVKVQPVSK
                     ELDYFTCLGFPRRLTIDPSALETKYYELSRAFHPDFYQNKSDEERAISLGNSAMLNTA
                     YRTLRDPIQRAEYLLDLEAGSAKDIRTSPPADLFEEIIELQEILDEYRATGHSSEAEP
                     TLRAKLQSERAILEQRQRDMEMQLQHLFAQWDELQDRGEATEEARAERDRILKQMREI
                     LSNRTYVRNIVNDLVATIA"
     gene            8108..9928
                     /gene="dnaK"
                     /locus_tag="VI359_04055"
     CDS             8108..9928
                     /gene="dnaK"
                     /locus_tag="VI359_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY6973458.1"
                     /translation="MTRIVGIDLGTTNSLVAYMDQGTPRVMPGRSGRTMVPSVVAMTD
                     NGLIVGDPAKEHLIRNPERTIYSVKRFMGKGLADVQGELAYFPYQLTEQGGVIRIKVG
                     EKFYSPPQISAMILKELKHRAEAHLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQQRTQGTIAVYDLGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDFDHRVADLFVKDILTQYGIDLTTSPDHMQAVRLEAEQVKVRLSTDLKTTATLEL
                     PDGKGRFTRELTRDQLESLTMDLVERTLAPCRLALKDAGLTPNDIDEIVLVGGSTRMP
                     LVQQRVQALFGKPPHCELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASTKEMFTTYVDGQTGVDIHILQGERELVKDNRSLARFRLKIPP
                     LPAGVPRIEVTFLIDANGILNVSARDIRTGETQSIAVKPSYGLADEEVERMIGESFKF
                     AAEDLKARQLIEANTEAEAILRATEKALKQGGHLISEQERSAIRASMLALESAKAGTD
                     HKVIRARMTELEQTTHHLAEILMDTSLKEALENKKLSEIT"
     gene            9956..10168
                     /gene="iscX"
                     /locus_tag="VI359_04060"
     CDS             9956..10168
                     /gene="iscX"
                     /locus_tag="VI359_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEY6973459.1"
                     /translation="MNLNWHDAEDIAIRLVEEHPDTDPLTVRFTDMHAWIVALPDFKD
                     DPKKSNEKILEAIQLAWHEEYKDSTS"
     gene            complement(10208..12604)
                     /locus_tag="VI359_04065"
     CDS             complement(10208..12604)
                     /locus_tag="VI359_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY6973460.1"
                     /translation="MTMIDRPVQLQDPPRQPPPHRPWRRWQIVLVGFGVLVLVGTIAA
                     AGVLWYFSQDLPSLDSLQNYQPSLVTQVYSSDQQLIGQFFIERRIVTPLAEIPKILSQ
                     AVIATEDARFFEHPGLDYIGILRAAWTNVRHGGKKLEGASTITQQLARSLFLSSERSY
                     GRKIRELILAYKMEIASTKEQILETYLNQIYFGQGAYGVASAAQTYFGKELAKLTLPE
                     AAFLAGLPKSPSRFSPFTAYDRAKKRQEHVLSRMEEAGFITTAEQEQAAAEPLAFRRP
                     GADQLAPYFAEYIRQLLIAKYGEAMVYKGGLKVFTTLNLEMQRVAEAAFAAGLRELDK
                     RQGWRGPLRTVDVATMPPPGTGAAADDTLNVGDYREGVVTKVAKDHCLVQMDHTVAKL
                     AFDDMAWAKKRLKGPDTTKDVVVEPNLKQILKPGDVIEVAVKKIDRGAISLQLEQTPL
                     VEGGLIAIDPGKGAIRAMVGGYDFNRSEYNRAVQAHRQPGSAFKPVIYATAINQGMSP
                     ASVILDAPVVYEQEQEEKTWKPENYGKKFHGMVSLRDALAHSHNLATVRLLEKIGVKN
                     VIDFAQTVGITSPLAADLSLGLGSSSVGLLELTSAYGVFLNQGSRVEPYAIFFVKDNA
                     GKVLELAEPEAHEVISKETAYLITNMMEDVIRKGTGQAAKVIDRPIAGKTGTTNEFIN
                     AWFIGGTPNLVTGVYVGFDDRRSLGETESGAHAALPIWIDFMRQALKQVPVVPFAIPD
                     GVTFVKVDPATGLLESEQEGTPGTVELFAKGSEPTQASQRRLDPTDFYKLDQITEGSP
                     "
     gene            12804..>14120
                     /locus_tag="VI359_04070"
     CDS             12804..>14120
                     /locus_tag="VI359_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6973461.1"
                     /translation="MDQSSLQSLSTSTRRPPLGHRADLRNVAIIAHVDHGKTTLVDAL
                     LRQTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVTYKGVKINIVDTPGHADFGG
                     EVERTLRMVDGVLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVV
                     NRTFDLFVHLGATDEQLDFPIVYTSAIKGVATLDVNKPGSDITPLLDTVLEKIPAPAI
                     NADAPLQILVLALAQDSYKGKMGVGKIQSGSIARRQNVLVLGKDRSQIPGKVSDLAVY
                     SGLERTDTEQAEAGEIVAVAGLDDVSIGDTIADAEHPLALPRVTIDEPTVQMTFSVNN
                     SPFAGREGKFLTSRHLRERLFKELETNVSLRVQETDSPDRFLVAGRGELHLGVLIEQM
                     RREGYELQVSQPEVILHRDGDKVTEPYEELTILIPETYQGAVIEEIG"
BASE COUNT         3249 a         4050 c         3828 g         2993 t
ORIGIN      
        1 agacatcatg attgaatgtc gacagtatcc gcgcgtccca gttgatctcc aagtcttctt
       61 ttccaccaca gggaatacgg atattcgaga agggacgatg ttcgacctct ccctgggggg
      121 gtgcgcagtg accactatga cctccgttca gccaggaacc ggtatccgta tcctgatccg
      181 ggcaactgat ctgggatctc ctattaccat cgagtctgca gcagtccgtt ggagccacca
      241 tggagaattt ggcgtcgagt tcatgggaat ctccgacatc gaccagagtc gtcttagccg
      301 ccttttgcaa gcatccacct cctatccgat ccgtccaagc tgaccattag accagcgcga
      361 tcttgagcgt gatagagtag tcggtatgag atgcccaatc tgccgaactc ccgccagctg
      421 gaaagagaac ccctggcgtc ccttctgctc tgagcgatgt caactgaccg accttggaac
      481 ttgggctgca gaacagtaca gatttccagg ctctcggctt actgtcgaac ccgaattaga
      541 ggaagagaca gattgagtca ggagtcatga cggctctcgc gaggtttcct ttctaagccc
      601 cgattggcaa gtccccacta tcttgataaa gttcccgtcc tacgtcagat acctgaaaag
      661 gggagtattg gatgatatca accaaaggct acgcagccct tagtgccaag gaagctcttc
      721 aacctttctc gtttgagcgg cgtcacgtcg gcgctcatga cctattgatc aagattagcc
      781 actgcggcat ctgccattcg gatattcatc aggcccgcaa cgaatgggga ggctctattt
      841 ttccgatggt cccaggccat gaaatcgtcg gtgtgatcac gcaaataggg aacgcggtca
      901 agaagttccg cgtgggcgat cgtgccggcg tcggatgttt tgtcgattcc tgccgaacct
      961 gcaccgcctg tctggaaggg cttgagcaat actgcgaagc cgggatgctc ctcacctaca
     1021 gtggccgtga ccaagacggt caacccaccc agggtggcta ttcgacgcag attgttgtgg
     1081 acgagaatta tgtcttgcgc cttcccagcg cgctctcagc agcagggaca gctccactac
     1141 tctgcgcggg ggttacgacc tactcgcctc ttcgccattg gggtgtgggc aagtatcaca
     1201 agttagcggt cgttggatta ggtgggctcg gccacatggc tgtgaaaatt gccagggcga
     1261 tgggcaccga agtcaccgtc ttgagcacgt ccgaacgcaa gcgaatggat gccgagaggt
     1321 taggcatcgc ccacttcgcg ttgacctcgc agcccgagac gttcacgaaa ctgcagcgac
     1381 acttcgattt catcctcgat accgtctctg ccccccacaa ctataatgcc tatgtgaatc
     1441 ttttgaaaac tgacggcacc atgattctcg tcggggcacc cgacaagccg acgccgctcg
     1501 aaccattccc cctcatcttg catcggcgcc ggatcgctgg atcggtcatc ggtggtatac
     1561 gtgagactca agaaatgctc gacttctgtg ccgaccatca tatcgagtct gatgtcgaag
     1621 tgattccgat acaacaggtg aacgaggcct acgagcgagt cattcgcggc gatgtgcgat
     1681 ttcgatttgt catcgacctg gcatcattga aataactgaa ccgacaggaa gtatattgct
     1741 ctgagaaaat ggctggggga ctatgaatcg aacctaggta gccagctcca aaggctggcg
     1801 tcctaccgct agacgatccc ccagcgcggt aaggaaccga gacatagacc tgaatagtac
     1861 ggcgtggcca agattagtgg ctgggggact aggaatcgaa cctaggtagt cagatccaga
     1921 gactgacgtc ctaccgctag atgatccccc aaccggagtt cacagtaata tatgcctcct
     1981 gattccgtca agatcggcgc gggtttgttg gaacacattc gttgattagg ctgatgcccg
     2041 ctcgattcct ttgcgcagcc gcagcagggt ccgctctcgc cccaacactt ccatgacttc
     2101 aaacagtccc ggactggcag tccgacctgt gagggcaacc cgaaccggct gagcgagctg
     2161 gcccattttc atgtcctctt cttcgaccag cttcttaaag ctcgcttccc actcctgttt
     2221 ggaaaaagtc ggtaaggctt caaaccgcgc caccaactta cccaagacag gcgccatcgc
     2281 cggcgtgagg aacttcttcg cagcctcttc gtcgaacgcg accgtctgcc caaagtaggg
     2341 cgtgacccac atgatcatgt cggtcagcgt tttagtccgc tccctgacta agacgacaag
     2401 ctgtgtcagc caccctcccg agacagtccg aacctcttct tgcaaaccgc tagcttccaa
     2461 gagcgggaga agcctctgtg caatctggct cggcgggctt gttttgatgt attcggcatt
     2521 catccagatc agcttctcgg gattaaacac cgccgcggat ttttgcacgt gatcccaaga
     2581 aaacttctca atcagttcct gacgcgtgaa gagttcctga tcgccatggg accagccaag
     2641 ccggaccaga tagttcatca tcgcctctgg gaggtacccc atttctttat aggccatgat
     2701 cgacgtggcc ccgtgtcgct tggagagccg ggccttatcc gacccaagaa tcatagggag
     2761 atgtccaaac tgcggcaccg gaaatcccag cgcctggaaa atcggcacct gccgcggtgt
     2821 gttggtcagg tgatcgtccc cgcgcacaac gtgggtaatg tgcataaggc catcgtcgac
     2881 gacgaccgaa aagttgtagg tcggatagcc gttcgatcgg aggataatca gatcgtccaa
     2941 gacggtgttg tcgaacacaa ccttgccctt gatgagatca tcgacgaccg tctgaccttc
     3001 ctgtggcgcc ttgaatcgca aggcggcttc ctcagttgga ttgatgactc ccagattccg
     3061 gcaacggccg tcgtagcgag gcgagagtcc tttcagttcg gcctctttcc gccgggcttc
     3121 gagctcctcc gccttacaaa cacaccaata ggcctggccc ttctcaagca actgcatcgc
     3181 gtggctgcgg tacaggtcca tgcggtcggt ttggcgatag ggcccctcgt cccaatccag
     3241 cccgacccac tccatgcctt cgatgatggc ttggatggat tcgtcggtgg accggctttg
     3301 gtcggtgtct tcaatgcgca ggatgaaaac gccgtgttgc tggcgggcat agagccaatt
     3361 gaacagggcg gttcggacac cgccgatatg caaaaaacca gttgggctag gcgcaaatct
     3421 gactcggacc tggctcatat tgtactcgca gcaggcggag taaaggtcgg tatctatagc
     3481 acgcactgaa aagggttgta cagaatctcg gcgcttccat ctagtcgcgg cttctgctat
     3541 gacgatgacg gagatcgtca tggcagaact cacacagtcg gccacggttc tctcggtgaa
     3601 cgacctcacc ccgcaggtgc gtcaattggt tctccttccc accgttcaca acattgcatt
     3661 tcagccaggc caatgggtat ctctcaagct gccggttggt gccaaaccgc cgcttaatcg
     3721 tgcctactca atggcggcac ccggtactcc atcgggtgaa cttactctgg tgttcgatcg
     3781 tgtgacaggc ggcttgggca ccacctatct ctacactctc aagcccggca gcgaagtaca
     3841 gttttcagga cctcacggaa actttgtgct tccgaagcaa cttgaccgag aactgatttt
     3901 gatcgctcgc tacaccggcc tcgtccccat acgctgtatg ttaaaacggc tttatgctca
     3961 gcggcagact gctcccatta tgctcattgc cgtcgctcct gcagaggaag aattgctctt
     4021 tcatcaagag ttactcacct tggcggtgac ccacccagga ttccgctacc tccccttggt
     4081 ggctgctgga ggggaacagc agggcgttga cctcacgctt tcgatgctcc gaccgttgat
     4141 agagggtgtg cctaaagtga ttcctatgtt gtgcggaacg aaaggattcg tacggccact
     4201 acgggcttat tttgttggga tggggtatga tcggaaggaa gtcaaaacgg aaacctatga
     4261 ttagattact ggtgtccttc tttcacaaga ttcttgttca cagccgggat ttcgaccacg
     4321 atatccttgg tgccgttcgg aacacattgg caggcaagac gtgattgcaa tgtcgtcatc
     4381 ggcgcctcat ccagctgatc gaactcgtca tcggttcctt cattgcagct ctctaaccct
     4441 tgcttcacaa tgacatggca ggttgagcag gcacagaccc ctccacaaac atgctccaat
     4501 tcgacaccgt tggccatcgc cacgtccaga acgctgccag gaagccccgt agggccgtag
     4561 ggaatcttgc cgggatcgac ctcaactttt gtcacctcgc cactcggggc aatgtaggta
     4621 atcgtatagg gtttgctggg gagttcgtag tcggctttct caatgtaggg gttggttcca
     4681 cccatgagcc tcgtcctttc gctgccaggt tcaatctccg atacccgtgt tgacagccct
     4741 gaaatctacg tggtattctt aatccgacca tattagtcgg agactattat agtcgccgat
     4801 aagatagatc ggcaatagcc atgttgaaga tttcaaaaaa agccgattac gccctgatgg
     4861 cgcttcagca catcgcctcc gtccaattcg gcgatgtgac gcccggtcgc gtggtaaaca
     4921 ccaaagaaat cgctgaagaa tacaatatcc cgctcgaact gctggccaaa gtacttcaga
     4981 cgttggccaa gcatggcatg atcgaaagtc ataacggtcc caagggcggc tacttactgg
     5041 cccgaagcgc gcgacaattg accattgccc agattctgga aagcattgaa gggccgctcg
     5101 gcattacgga ctgttctcat gaaaaagacg gcgagctgtg tctccagctg gagcattgta
     5161 ccattcgcac accgctgctg aaggtccaag acagcattta tcagcttctg aataatatga
     5221 cgctgcaaga catgatgggc ggcacgccgc tcattacgat tcagtcccct acgacccaag
     5281 gagtcgaacg atgaaattgc cgatcttttt ggataaccat tccactacgc cgatggatcc
     5341 gcgtgtgctg gagaccatgc ttccctattt tgtcgagaaa tttgggaatg ccgccagtcg
     5401 caaccatgcc tttggatggg aagcggaaga agcggtcgaa caggcccgta agcagatcgc
     5461 caagctgatc aaggccgacc ccaaggagat catcttcacg agcggcgcca ccgaggcaga
     5521 caacctcgcg attaaaggcg tcctggaaat gtacagagag aagggcgatc acattatcac
     5581 gtcgtccacc gaacatcgcg cggtcctcga taccgcgaag gccatggagg ccaaaggtaa
     5641 ggcgacagta acgtacctcc cggtcgataa atacggaatg gtgaatcctg aagatgtgcg
     5701 ccacgcgatc acggagaaga cggttctcat ctccatcatg ctggccaaca acgaaatcgg
     5761 cacgatcaat ccgatcaaag agatcgggaa gattgccaag gaaaaaggcg tcctctttca
     5821 ctgcgatgcg acgcagggtg tgggtaagat tccggtcgat gtgcaggaca tgggtatcga
     5881 tctaatgtcg ttcaccgcgc ataagatcta tggccccaaa ggggtgggtg cgctgtatgt
     5941 acggaagaag aacccacgtg ttcgcatcgc ggctcagatg gatggcggtg gacatgagcg
     6001 cggtatgcgg tccggtacac tcccggttcc gttgatcgtc ggattcggca aagcctgtga
     6061 attgtgcgga ctggaaatgg ccacggaatc ggttcggctg gccgccatgc gcgatcggct
     6121 ccacgttggt atcacgaagg tgctcgaaga gagttattta aacggccacc cgacgaatcg
     6181 gttacccggc aatctgaata tctcgtttgc atatgttgaa ggcgagtcct tgttgatggg
     6241 tatgaaagat attgccctgt cctccggctc ggcctgcact tcggcaacgt tggaaccctc
     6301 ctatgtgctg cgggcgctcg gcgtcggcac ggagttggct cattcgtcga tccgcttcgg
     6361 cctgggccgg ttcaatacag acgacgagat tgactacacg attaaaaagg ttattgaggt
     6421 cgtgaccaag ctgcgagaaa tgtctccgct gtatgaaatg gcgaaggaag gcgttgattt
     6481 gaaatctgtt cagtgggcgg cccactgagc cacgagcaga tgaaggagga gtgaccatgg
     6541 cctatagtga caaagtcgtt gatcatttca ataacccccg caatatgggc agcttcaaga
     6601 aggatgagga aggcgtcggc accgggatgg tcggcgcgcc ggaatgcggc gacgtgatga
     6661 agctccagat caaggtgcag aacgatacga tcgtggatgc caagttcaag acattcgggt
     6721 gcggctccgc gatcgccagt tcgagcctcg cgacggaatg gctcaagggg aagaccgtcg
     6781 aggaagccca gaaaatcaag aacaccgaca ttgtgcagga gctcaatctg ccaccggtga
     6841 agattcactg ttcggtgctg gccgaggatg ccatcaaggc cgcgctggcc gattaccaga
     6901 aaaaggccga tgggaagaca gtcgacacga agtaacgaca cgaaggagcc acgcatggac
     6961 gccacgaacg cagagacgca agctccggtc attacactga gcgaagccgc cttgaaggaa
     7021 gtgaagcggt tgatcaacgt gcaaggcctc accgagggtg gcctgcgtct gggtgtcaaa
     7081 gggggcgggt gctcggggtt gagttacacc atcaatttcg acgacaagat tgggcagtac
     7141 gaccaggtct acgagttcga cggcgtgaag gtgattgtcg acgtgaaaag cgcgatctac
     7201 cttcagggaa cgcagttgga cttccagaaa gatctgatgg gcggcaattt caagttcgtc
     7261 aatccgaacg ccaataagac ctgcggctgc ggagagtcgt tctcggccta acctcggcac
     7321 gcttccacga cgacatgttc accgcgggca tggcaggtcg ccatgcccgt gctgtcagag
     7381 cagagaccat ggacccacac actcatatga cgagtcgtcg cgaactccaa atggctcgca
     7441 gcatgtgctg gcactgtcaa tccgaagtcg ccggcgagta cttctgtgag cggtgcgtaa
     7501 aggtccagcc ggtttcaaaa gaactcgatt atttcacttg tctggggttt cctcgtcgcc
     7561 tcaccattga tccgagcgcc ctcgaaacca aatactacga actgagccgg gcattccatc
     7621 ccgattttta ccaaaacaag agtgatgaag aacgagccat cagcctgggc aactcggcaa
     7681 tgctcaacac cgcctaccgg acgctacgcg atccgattca gcgggccgaa tatctactgg
     7741 accttgaagc cggttcagca aaggacatcc ggacgtctcc tcccgccgat ctattcgagg
     7801 aaattatcga gctgcaggaa atcttggacg agtaccgcgc cacaggccat tcctccgagg
     7861 cagagcccac gcttcgcgcg aaactccaat ccgagcgagc catacttgaa caacggcagc
     7921 gggacatgga aatgcagctg caacaccttt tcgcgcagtg ggacgagctg caagatcgtg
     7981 gagaggccac cgaggaagcc cgggccgaac gagaccgcat cctgaaacag atgcgggaaa
     8041 ttctatccaa tcgaacctat gtcaggaaca tcgtcaacga cctcgtcgca accatcgcat
     8101 aaccaccatg acacgaatcg tcgggattga tctaggaaca acgaattccc tcgtcgcata
     8161 tatggaccag ggtacgccgc gcgtcatgcc tggacggagc ggccgtacca tggttccatc
     8221 ggtcgttgcg atgaccgaca acgggcttat cgtcggcgat ccggcaaaag aacacctcat
     8281 tcgcaatcct gaacggacga tctattccgt caaacggttc atggggaaag gcttggccga
     8341 cgtgcaaggc gagctggcct acttccccta tcagctcaca gaacaaggcg gggtcatccg
     8401 gatcaaggtc ggcgagaaat tctactcgcc accgcagatc tcggcgatga ttctgaagga
     8461 actcaagcat cgcgccgaag cccatctcgg tgagagcatc acgaaggcgg tcatcactgt
     8521 gcccgcctat ttcaatgaca gccagcgaca agccacgaaa gacgccggca tgatcgcagg
     8581 gctcgaagtg ctccggatca tcaacgaacc gaccgcggcc tcgctcgcgt acggattgca
     8641 gcaacggaca cagggcacca tcgctgtgta tgacctcggc ggcgggactt tcgacatctc
     8701 cattcttaaa ctcaagaacg gcattttcga agtgctcgcc acgaacggcg acacccatct
     8761 tggtggcgac gattttgacc accgtgtcgc tgatcttttt gtgaaagata ttctcacaca
     8821 gtatgggatc gatctcacca cgtccccaga tcatatgcag gcggtgcggc tcgaagcgga
     8881 gcaggtcaaa gtccgacttt ccaccgatct caagacgacg gctacgcttg aattgcccga
     8941 cggcaagggc cgctttacgc gtgagctgac ccgcgatcaa ctcgaatcct tgaccatgga
     9001 cctcgtcgaa cgaaccctgg ctccatgccg ccttgcgctg aaagatgcgg gcctcacgcc
     9061 caacgacatc gatgaaatcg tcctcgtcgg aggatccacg cggatgccgc tggtccagca
     9121 acgggtccag gcgttattcg gcaagccacc ccactgtgaa ttgaatcccg acgaagtcgt
     9181 ggctcttgga gcagctgttc aggcggatat tcttagcggt gggacgaccg acatgttgtt
     9241 gctggacgtg accccgctct cccttggcat cgaaacgatg ggcggagtga tgagcagcct
     9301 aatccgccgc aacacgacca ttcccgcaag cacgaaagag atgttcacga cctatgtgga
     9361 tggccaaacc ggtgtggata ttcacattct gcaaggtgaa cgagaactgg tgaaagacaa
     9421 tcgcagcctc gcacggtttc ggctcaagat ccctccgctg ccagccggcg tcccacgtat
     9481 cgaggtcacg tttctaatcg atgcaaacgg gattcttaat gtctctgcac gagatatacg
     9541 cacaggcgaa actcagtcaa ttgcagtcaa accctcgtac ggcctcgccg acgaggaagt
     9601 ggaacgcatg atcggcgaat cattcaagtt tgcggctgaa gacttgaaag ccaggcaact
     9661 aattgaggcc aataccgaag cagaggctat tttgagggcc acggagaaag cccttaaaca
     9721 gggtggacat ttgatcagtg aacaagaacg ctctgccatt agggcatcga tgctcgccct
     9781 cgaatcagcc aaggcaggaa cagaccataa ggtgattcgc gccagaatga ccgagcttga
     9841 acaaactacg catcatctcg ctgaaatcct gatggatacg tctttaaagg aagcgttaga
     9901 gaacaagaag ctatcggaga tcacgtagcc gtcagctttc agcaaggagc aggccatgaa
     9961 tttgaactgg catgatgcgg aagacatcgc aatcagattg gtggaagaac atccagatac
    10021 ggatccgctt actgttcgct tcaccgatat gcatgcgtgg atcgtggcgc ttccagactt
    10081 caaagacgac ccgaagaaat cgaatgagaa gattctggag gcgattcaat tggcctggca
    10141 cgaagagtac aaagattcca cgtcataaca atctcctagc cgccactttt tgccgacagc
    10201 cccctgccta gggcgagccc tcggttattt gatccagctt ataaaaatcc gttggatcca
    10261 accgccgctg gctcgcttgg gtgggctcac tcccctttgc aaagagttcc accgtcccag
    10321 gtgttccttc ctgttccgat tccagcagcc ccgtggccgg atcaactttg acgaaggtca
    10381 cgccgtcggg aatcgcaaag ggtaccaccg gcacttgttt caacgcttgt ctcataaagt
    10441 cgatccatat cgggagcgcc gcatgggcgc ctgattcggt ttcgccgagg gagcgtcgat
    10501 cgtcaaatcc cacatagaca cccgtcacga ggttcggcgt cccaccgata aaccaggcgt
    10561 tgataaattc gttggttgtt ccggtcttcc cagcgatggg ccgatcgatg actttcgcag
    10621 cttgtccagt ccctttacga ataacgtctt ccatcatatt ggtaatcaga taggcggtct
    10681 ctttcgagat cacttcgtgc gcttccggtt ccgccagctc gagcaccttg cccgcattat
    10741 ccttcacgaa gaaaatggca tagggctcca ctcggctgcc ttgattcaag aacaccccgt
    10801 aggctgaggt caattccaac aacccaacgg aagaagaccc cagccccaag gaaagatccg
    10861 ctgccaatgg actggtgatg ccgacggtct gagcaaaatc gatcacattc ttgaccccga
    10921 tcttctccaa caggcggacg gttgcaagat tgtgcgagtg cgccaaggcg tcccgcaaac
    10981 tcaccatccc gtggaacttc ttgccatagt tctccggctt ccacgtcttc tcttcctgtt
    11041 cctgttcgta tacgaccggc gcatcgagga tcaccgacgc aggactcatg ccctgattta
    11101 tcgcggtggc ataaataacc ggcttaaatg cggagccagg ttggcgatgg gcttgcaccg
    11161 cccgattgta ttcgcttcgg ttgaaatcgt acccgcccac catggcgcgg atggcgccct
    11221 tccccggatc tatcgcaatt aagccgccct cgaccagcgg cgtttgctcc agctggagcg
    11281 aaatcgcccc gcggtcgatc ttcttgacag ccacttcgat cacgtcacct ggtttcaaga
    11341 tctgcttcag attcggctcg acgacgacat ctttcgtggt atccggtcct ttcagccgct
    11401 tcttggccca cgccatatcg tcaaatgcca acttggcgac cgtatgatcc atttgaacga
    11461 ggcaatgatc tttcgccact ttcgtgacca ctccctcccg atagtcgcca acgttcagtg
    11521 tgtcatcggc cgcagcccct gttccagggg gaggcatcgt cgcgacatca acggtccgta
    11581 gcgggccacg ccacccctgg cgtttgtcga gttctcgaag cccagcggca aatgccgctt
    11641 ctgcaaccct ttgcatttcg aggttgagcg tcgtgaacac cttgagtccg cccttgtaca
    11701 ccatcgcctc gccgtacttg gcgatcaaca actgccggat gtactccgcg aaatagggcg
    11761 cgagctggtc agcgccagga cgtctgaagg ccagcggctc ggcagccgct tgttcttgct
    11821 ccgccgttgt aatgaacccc gcctcttcca tccggctcaa cacatgttcc tgccgcttct
    11881 ttgcgcggtc ataggccgtg aatggagaga accgactcgg cgatttcggc agtccggcaa
    11941 gaaacgccgc ttccgggagc gtcagtttgg cgagttcttt cccgaaatag gtctgggccg
    12001 ccgaggccac gccatacgca ccttggccaa agtagatctg gttcaaatag gtttcgagga
    12061 tctgttcctt cgtcgacgcg atctccatct tataggccaa aatcaactcg cgaatctttc
    12121 gaccgtacga ccgttccgac gacagaaaca gtgatcgcgc caattgctgc gtgatcgtgc
    12181 tcgctccttc aagtttcttt cctccatgcc gaacgttggt ccaggctgct cgcaagatcc
    12241 cgatatagtc gaggcccgga tgctcgaaga accgggcatc ctcggtagca ataacggctt
    12301 ggcttaaaat cttcgggatc tccgccagcg gcgtcacgat gcgacgttcg atgaaaaact
    12361 ggccaataag ctgttgatcg ctggaataga cttgggtaac cagactcggc tgatagtttt
    12421 gcaacgaatc cagcgagggc agatcttgcg aaaagtacca gagcacgccg gcggcggcga
    12481 tggttccgac cagaaccaag accccgaatc ccaccaggac gatctgccac cggcgccacg
    12541 gccggtgcgg cggcggctga cgaggcggat cctggagttg tacgggccgg tcgatcatgg
    12601 tcatggattg atgcccccgt gagggctcgg tcatcggtag gatgatgtag attcaccata
    12661 ccccggcaag ctctaaaact caagcagagg acgaccctgc acccgcgctt gtgccaggtg
    12721 tgacccttca ggtatactgt gagtcgacgc ctggagttcg gggcgtcttt tttttcgtgt
    12781 tcaaacggat cagctgagtg accatggacc agtcttctct acagagcctc tcgacatcga
    12841 cgcgccgacc ccctctgggg caccgggctg atctccgcaa tgtcgcgatc atcgcccacg
    12901 tcgaccatgg caagacgacg ctagtcgatg ccctgctccg tcaaacccac gtccatcgca
    12961 agatcgacga tatgggtgaa cggatcatgg actcaatgga tcaggagcgc gaacgcggca
    13021 ttaccatccg agccaagaac gccagcgtca cgtacaaagg cgtgaagatc aacatcgtcg
    13081 acacgccggg ccacgccgac ttcggcggtg aagtggagcg aaccttgcgg atggtggacg
    13141 gcgtgctgat cctgattgac gccaaagaag gaccgatgcc gcaaaccacc tttgtcttgc
    13201 gaaaagcctt ggcgctcggc cacaaggcca tagtggtcat caacaagatc gaccgtccgg
    13261 acgccgtcat cgatgacgta gtaaaccgaa ccttcgatct cttcgtgcat ctcggtgcaa
    13321 cggacgagca gctcgacttc cccatcgtct acacctcggc catcaaaggc gttgcgacac
    13381 tcgatgtgaa caaaccggga tcggacatta ctcccctcct cgacacggtg ctggagaaga
    13441 ttcccgcgcc ggcgatcaat gccgacgccc cactccagat cctagtcctc gccctggccc
    13501 aggactccta taaaggcaag atgggagtgg gcaagattca gtccggttcg attgcgcgca
    13561 ggcagaatgt gctcgtgctc ggcaaggaca gatcccagat tcctggcaag gtgtcagatt
    13621 tggcggtcta ctccggtctt gaacgaacgg acacggaaca ggcggaggcg ggagaaatcg
    13681 tcgcggtcgc cggccttgac gacgtgagta tcggcgatac gattgccgac gcggagcatc
    13741 cgcttgcgct gccccgtgtc acgatcgacg agccgaccgt gcaaatgacc ttttccgtca
    13801 acaacagccc cttcgccgga cgcgaaggca agttcctcac ctcccgtcac ttgcgtgaac
    13861 gcctgttcaa agaactggag accaacgtct cgctgcgggt gcaagaaacc gacagccccg
    13921 atcgattcct cgtcgcaggt cgaggcgaac tccatcttgg cgtgttgatc gaacagatgc
    13981 ggcgcgaggg ctatgagctc caggtctcgc agccggaggt catcctccat cgcgatggcg
    14041 ataaagtcac cgagccctac gaggaactga ccatactgat tccggaaacc tatcagggcg
    14101 ccgtgatcga ggagatcggc
//
LOCUS       DATDNW010000165         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_159980, whole genome shotgun sequence.
ACCESSION   DATDNW010000165 DATDNW010000000
VERSION     DATDNW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159980"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2208
                     /gene="rpoC"
                     /locus_tag="VI359_04075"
     CDS             <1..>2208
                     /gene="rpoC"
                     /locus_tag="VI359_04075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY6973462.1"
                     /translation="FCAKIFGPIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRE
                     RMGHIELAAPVAHIWFLKGVPSRIGTLLDMSLKQLEKILYFESYVCVDPGTTDLVEKD
                     LVTEEKLRSLQSEYSAGSYKVGIGADAIRELLRKIDINTLWDELHVKAKASASAALKK
                     KYAKRLKVIEAFRKSGNKPEWMIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVI
                     NRNNRLKRLIELKAPGVIIRNEMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDML
                     KGKQGRFRQNLLGKRVDYSGRTVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERG
                     AATTIKSAKRLVEKERPEVWDVLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAI
                     RLHPLVCAAFNADFDGDQMAVHVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQD
                     MVLGCYWLTKERVGVKGEGKVFGSPEEVRIAFDAREVEEHARIKVRIEGALVQTTVGR
                     VLLSEVLPPGMPFANANKVMTKKEVTRLIDTVYRQTGHRETVTFLDKIKDIGFQYATR
                     AGVSICIDNMHIPTKKEDLIGKAQREVNEIERQYSEGLITNGERYNKVIDIWAHVTEQ
                     VANEMMKELGAGGDPAKVESFNPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIE
                     TPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDIAQDVIIN"
BASE COUNT          517 a          565 c          688 g          438 t
ORIGIN      
        1 ttctgcgcca aaatttttgg gcccatcaag gattgggaat gcaactgcgg caagtacaag
       61 cgcatgaagc accgcgggat tgtctgcgac aagtgcggcg tggaggtcat tcaatcaaag
      121 gtacgccgcg aacggatggg ccatatcgaa ctcgctgcgc cggtggctca catctggttt
      181 ttgaaaggcg tccccagccg tatcggcacg ctcctggaca tgagcctgaa gcagctggag
      241 aagattcttt atttcgagag ctacgtctgt gtcgatcccg gaacaaccga tttggtggaa
      301 aaggatctcg tcaccgaaga gaagctgcgt tcactccaat ccgagtacag cgcagggtcg
      361 tacaaagttg ggatcggtgc cgatgccatc cgtgaactgc ttcggaagat tgatatcaac
      421 accctgtggg atgagctgca tgtgaaggcg aaggcctcgg cctccgccgc gctgaagaaa
      481 aagtacgcga agcgactgaa agtcatcgaa gcattccgga aatccgggaa caagccggag
      541 tggatgatca tggatgtgat ccccgtgctt ccaccggagt tgcgcccgtt ggtgccgttg
      601 gacggtggtc ggtttgcaac gtcggatctc aacgatctgt atcggcgcgt cattaaccgg
      661 aacaatcggc tcaaacgact gatcgaattg aaagcccccg gcgtcatcat ccgtaatgaa
      721 atgcggatgc tgcaagaagc ggtggatgcc ctctttgaca acgggcgtcg aggccgcgcc
      781 attcgtgggc ccaacaagcg tccgctgaag tcactgagcg atatgctcaa gggcaaacag
      841 gggcggttcc gacagaacct tctcggaaag cgcgtggatt actcggggcg aaccgtgatc
      901 gtggtggggc cggaattgcg gttgcaccag tgcgggttgc caaagaagat ggcgctcgag
      961 ctgttcaagc cttttatctt ccacaaattg gaagagcgcg gggcggcgac gacgatcaag
     1021 agtgccaagc gtctggttga aaaagaacgg cctgaagtgt gggacgtgct ggacgaggtc
     1081 attcgtgagc accctgtgct cttgaaccgt gctccgacgc tgcaccggtt ggggattcaa
     1141 gcgttcgatc cggtgttggt cgaaggcaaa gccattcggc tgcatccgct ggtctgcgcc
     1201 gcgttcaacg ctgacttcga cggcgaccag atggcggtgc acgttccgct gtcggtggaa
     1261 gcgcaggtcg aagcccgtgt gctgatgatg tcgattaaca acatcttgtc tcccgcgaac
     1321 ggaaaaccga tcgcggtgcc gtcccaagac atggtgttgg gctgctattg gttgacgaag
     1381 gaacgggttg gggtcaaggg cgagggcaag gtgttcggct cgccggaaga agtgcgtatc
     1441 gcctttgatg cccgggaagt cgaagagcac gcacgtataa aagttcgcat tgaaggggcg
     1501 ctggtgcaga cgacagtcgg ccgtgtgctg ctgtccgagg tcctccctcc tgggatgccg
     1561 ttcgccaatg cgaacaaggt gatgacgaag aaggaagtga cgcggctgat cgatacggtg
     1621 tatcgccaaa ccggacatcg ggaaaccgtg acctttctcg acaagatcaa agacatcggg
     1681 ttccaatatg cgacgcgcgc tggcgtgtca atctgcatcg acaacatgca tatcccgacc
     1741 aagaaagaag atctgatcgg gaaagcgcaa cgcgaagtaa atgaaatcga acggcagtat
     1801 tccgaggggc tgattacgaa cggcgagcgc tacaacaagg tcatcgacat ttgggcccac
     1861 gtaaccgagc aggtagccaa cgagatgatg aaagagctcg gggctggagg tgatccggcg
     1921 aaagtcgaat ccttcaaccc gatcttcatg atggccgact cgggtgcgcg aggcagttcg
     1981 caacagattc gtcagctggg tgggatgcgt ggattgatgg ccaagccatc cggcgaaatt
     2041 atcgaaacgc cgattaccgc gaacttccgc gaaggcttga ccgtgttgca gtacttcatc
     2101 tcgacgcacg gtgcacgtaa aggcttagcc gatacggcgt tgaagaccgc gaactcgggc
     2161 tacctcacgc gtcgtcttgt cgacatcgcg caagatgtca tcatcaac
//
LOCUS       DATDNW010000166        10743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_439315, whole genome shotgun sequence.
ACCESSION   DATDNW010000166 DATDNW010000000
VERSION     DATDNW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439315"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VI359_04080"
     CDS             <1..733
                     /locus_tag="VI359_04080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY6973463.1"
                     /translation="VAIKTMRLDEIDDDEKLRDFKARFFREAESTGRMSHPNIVTIYD
                     AGEEQNLAFIAMELLEGTPLKQYARKPNLLPPKDVVSMLATVADALDYAHQQGVVHRD
                     IKPANIMITKDQTVKVMDFGIAKMASTNKTQTDIVLGTPTYMSPEQIAGKKVDGRSDI
                     FSLGIVLFELLAGQPPFTADNLSALLFSITHNPHPTIQSFRKDLPPVMQEILDRALQK
                     ELPQRYRRAGEFAQDLRACLQSLAA"
     gene            746..1558
                     /locus_tag="VI359_04085"
     CDS             746..1558
                     /locus_tag="VI359_04085"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY6973464.1"
                     /translation="MPIEILHCAKTDVGLKRHRNEDRFCANHDLGLYVVCDGMGGENA
                     GDIASTLAVNTIQTYMADTARLSDLPFVGPYDATVSPMTNRLASAIRLANQTIHQESW
                     TQPDCVGMGTTVVAAHLSNEMLSIAHVGDSRLYLVRSNTIHGLTTDHSWVADQVLNGL
                     LTEEEAEQSSRKNIVTRALGVESTVEVELNEVAVMSGDVFLLCSDGLTRGVRTPEILR
                     ALRDEHTVESKANRLIAMANQAGGDDNITVLVVTVQQDDRPGLWERFRTGFL"
     gene            1595..2311
                     /locus_tag="VI359_04090"
     CDS             1595..2311
                     /locus_tag="VI359_04090"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY6973465.1"
                     /translation="MPHTAIAHAKLLVKLHGQASRMIDLVTEGFAIGRKGDNDLAIDD
                     QSISSHHARIVKVQSVYFIEDLKSTNGTSVNEKRIERHQLRDGDIISIGQHRLIFQDI
                     SAAETTPAAVSIDMDKTMVLAGGSQLQGLAAPAARLLVTSGKTDQMEYTLAKQVALIG
                     SQQDATIRLTGWFVPKAAARVTRRGLQYSISPAQRTKKVLVNGKEVVTQQELKNGDQI
                     EVAGVTLSFFLTPSSKTQRR"
     gene            complement(2322..2951)
                     /locus_tag="VI359_04095"
     CDS             complement(2322..2951)
                     /locus_tag="VI359_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HEY6973466.1"
                     /translation="MTQQGSSGRVYLWLVTALSLGLVWGTIVSSFPSQQELTAIDPPR
                     AAAASVPPPATVPTLDLTIPTSPPLPSQDQSAVQKESAPAQRPQAGISTGMASDPRSQ
                     QVTKLKCEAEIEQFCPASSDGPMRRQCIEQRVSQLAPRCQQLIRERFVKWREERTRMI
                     AACQEDVRRYCMSMTPGSGNMVQCLQDHAQDVSDRCYQTLPKGTVYFKH"
     gene            complement(3086..4099)
                     /locus_tag="VI359_04100"
     CDS             complement(3086..4099)
                     /locus_tag="VI359_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973467.1"
                     /translation="MLSWSRPDPLTRDLIHLINARRHDHGDTGAAIDTLQAFIEQGRE
                     HDLLTVLAALDEDMAEWLFDLLAESACTVLLDEEADEKPLYAVMAALALPLQANLSHP
                     LKLKIDPVATANLLHRALRLPAGTVVRVESRLLTDATLEPLTLQSVNEHVNAVADSQR
                     AQTIAARLYPPVENTAFLLFELIGAPDAGLPDSMEDRHRRALSEGLVEQCPELAFAPD
                     AIEAPVYAAYALFDAQNAVRLHRLADETAAVWRDLDPLVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPEEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVET
                     PEN"
     gene            complement(4236..4649)
                     /locus_tag="VI359_04105"
     CDS             complement(4236..4649)
                     /locus_tag="VI359_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY6973468.1"
                     /translation="MKSYREELWFETKTRRAFVNITPQVETVVRKSGVVEGLVLVNAM
                     HITASVYINDDEPGLLQDYERFLEAIAPQAASYRHNETGEDNADAHIKRQLMGREVVV
                     AISKGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            4769..6541
                     /locus_tag="VI359_04110"
     CDS             4769..6541
                     /locus_tag="VI359_04110"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY6973469.1"
                     /translation="MPRPGTSGKGGPPKTPSLRLSANALTVLRRRYLAKSPTGTVIEK
                     PAQLLWRTASDIALAERLYPANGRLPHAARRFYELMARLEFLPNSPTLMNAGRRLQQL
                     SACFVLPVEDSLDSIFEAVKFQALIHQSGGGTGFSFSHLRPKDDRVASTSGVASGPVS
                     FMRIFNMATDVIKQGGTRRGANMGVLRVDHPDILEFIGIKQNLNEMTNFNLSVGITDA
                     FMQAVERNRTYALINPHDGSTAQHLSARMVFDRLVEAAWATGEPGMIFLDTVNRANPT
                     PQLGTIESTNPCGEQPLLAHESCTLGSINVARFVSPSRTLPTVDYERLGATIALAVRF
                     LDNVIDRNHYPLSQIAAATRRTRKIGLGIMGFADLLIALNIRYDSPEALTVAESLMKF
                     FQARAHEASAQLAAVRGTFPAYRASRLSTMGQRRRNATVTTIAPTGTISIIADCSPGI
                     EPLYGVRVVRTVMEDVRLTGLHPMFAHRARVLGLDLAKLERDVAAHESIQHLTQIPAG
                     LRRLFVTAHDVAPEQHVRMQAVFQRYSDSGVSKTINLPATSTQADVASAFRLAYQLGC
                     KGLTVFRSGSREKQVLSCTQILAC"
     gene            6674..7384
                     /locus_tag="VI359_04115"
     CDS             6674..7384
                     /locus_tag="VI359_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY6973470.1"
                     /translation="MAAFTDCLTMPGTPFQWSLLFARQPEPDLEFTEEELEQTTTTRP
                     MSPMKPGKKSGGGRPIMWVLLLILVGGIAYVAMEPEMLTELLNPILGENAPVAGPSPR
                     VMTPKPQPPATAAPVPAPTPEPAPAVAAPAPPAPAMTPPGAAPAPTPTAQPAVTAIPM
                     FAEGQKVTVTSDPTAPGEGIPLSTDSSGAKPGAIVKPGVTLTVLDGELQGNSWVYSVR
                     TDEGMKGWVPEKRLRLKF"
     gene            7496..8212
                     /locus_tag="VI359_04120"
     CDS             7496..8212
                     /locus_tag="VI359_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY6973471.1"
                     /translation="MGQLAAVIFDFDGVIADTEPLHFAGFRRTLAEIGITLTESDYYA
                     NYLGYDDRGCFNAALEARGHPTNPIVISRLMERKAQAYLESIREDLIIFPGVHELVRE
                     AAASHPLAIASGALRHEIELILEQAGLRKQFSHITSAEDVTRGKPDPQPFFQALQGLN
                     RLQRGREIAPEACLVIEDSIPGIRAGKAAGMKVLAVANTHTLQDLHEAHAVAHSLSEV
                     RLSELHERLWPDRLMDPKRR"
     gene            8176..9069
                     /gene="hpnD"
                     /locus_tag="VI359_04125"
     CDS             8176..9069
                     /gene="hpnD"
                     /locus_tag="VI359_04125"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY6973472.1"
                     /translation="MARSIDGPEEAMTPAEAQAYCTAFTKKSGSNFYYSFLFLPRARR
                     HAMYTVYAFCKEVDSAVDEPSPGSNPREELRRWRMELEAAYHGTPTFPVTISLAEHAR
                     RLSIPQAYFEELIKGVEMDLMMPNYKTFRDLSLYCYRVASVVGLICLHVFGTTSARAQ
                     DYAVDLGMAFQLTNILRDLGTDAAQGRVYLPEEDLEKFGCSAEMLQRQQDSPPLRELI
                     RFEAARAHAYYDKAAAALEALPAKDRRALTVAEIMRAVYFRMLRRIEQAEYHPFGPRV
                     RLSTTSRLAVAAGVWLRSRFS"
     gene            9045..10400
                     /locus_tag="VI359_04130"
     CDS             9045..10400
                     /locus_tag="VI359_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6973473.1"
                     /translation="MASLSILVTRPTPSSVIVLGGGLAGLTAAYRLASRGTHVTLLEL
                     RSTLGGNSIPPGKGWEPSPLTISNGHQATWDLLRSLGSHISPAHFSHIPLEFLMPDGT
                     TTTYPYTPLPQPAHVAVSLLRFNGLTWRERWRLVSWLEQIWEGAVPLPSDLAHRTADE
                     WLAALGQGECARRNVWDPLARWLTGNQLTHQSADTFRGAIEQTFFRSARESRWAIVPS
                     FQALLVQPMIEALRTSGATVLLDTEATHLLSEGDRVTGVLLRNGSTLQGDWYLAALPP
                     QHLARLLPERWLSRYAYFQQLAELITRPTISAQIIIWHALTQPRILLLSGGFFYSMVA
                     QPSGSGHTVCRFFGGDEAGADSMTSDTLLATAEEVLRSLHMLAPGQIVESCDSQRNEE
                     GCLSLEPGVQLRRPLQRSPIANFLVAGDWTDTGWPPNNNVEGAIVSANRCADIITSSP
                     A"
BASE COUNT         2383 a         3173 c         2899 g         2288 t
ORIGIN      
        1 cgtcgccatc aagaccatga ggctcgacga aatcgatgac gacgagaaac tgcgggattt
       61 caaagcccga tttttccgtg aagctgaatc gaccggacgc atgtcccacc ctaatatcgt
      121 cacgatctat gatgcaggag aagaacagaa cttagccttt attgcgatgg aactgctcga
      181 gggaaccccc cttaagcaat atgccaggaa accgaacctg ctccccccaa aagacgtcgt
      241 ctcgatgctc gcaacggttg ctgatgccct cgattacgcc catcagcaag gcgttgtcca
      301 tcgagacatc aaaccagcca acatcatgat cacaaaggat cagaccgtga aagtgatgga
      361 tttcggcatc gccaagatgg catcgaccaa caagacccaa actgacatcg tactcgggac
      421 gccaacctac atgtcgccgg agcagatcgc cggcaagaag gttgacggac gctccgacat
      481 cttttcgctg ggaatcgtcc tgttcgagct tcttgcggga cagccaccgt tcacagcaga
      541 caacttgtca gctctactct tcagcatcac gcataacccg catcccacga ttcagtcgtt
      601 tcggaaggat cttcccccgg taatgcagga aattctcgat cgagcattac aaaaagagtt
      661 gcctcaacga taccgtcgtg cgggagagtt cgcccaggat cttcgggctt gccttcagag
      721 cctggccgct tgagaagggt ccttcatgcc gattgagatc ctccactgcg ccaaaacgga
      781 tgtcggactc aagcgccacc gcaacgaaga ccggttttgc gcgaatcacg atctcggact
      841 gtacgtcgtc tgcgacggaa tgggcggaga aaatgccgga gacatcgcca gcacccttgc
      901 ggtcaacacc attcaaacgt atatggcgga cacagcccgt ctatccgacc tgcccttcgt
      961 cggtccgtac gacgccaccg tctcccccat gacgaatcgg ctcgccagcg ccatcagact
     1021 ggccaatcag accattcatc aagagtcctg gacgcagcca gactgtgtcg gcatgggtac
     1081 caccgtggtg gcggcacatc tctccaacga gatgctctcc atcgcccacg tcggagacag
     1141 ccgactctac cttgtgcgca gcaacaccat tcatggattg accacggatc attcgtgggt
     1201 ggccgaccaa gtcctgaacg ggcttttgac ggaagaagag gcggagcagt catcacgtaa
     1261 gaacatcgtc acgcgtgcgc tcggcgtcga aagtaccgta gaggtcgagc tcaacgaagt
     1321 ggcggtcatg tcgggcgatg tgttcttgct ctgttccgac ggcctgaccc gaggcgtccg
     1381 aactccagag attctccggg cactccgtga tgagcacacc gtggaatcga aagccaatcg
     1441 attgatcgcg atggccaacc aagccggggg agatgataac atcaccgttc tcgtcgtcac
     1501 ggttcaacaa gacgatcggc ctgggttgtg ggaacgattt cgcacaggat tcttgtgacc
     1561 atatgagctg agactgaaag aggagggatc accgatgccg cacactgcga tcgcacatgc
     1621 gaagctgctc gtcaaattac acggccaagc ttcgaggatg attgacctcg tgacagaggg
     1681 ctttgccatc ggacgcaagg gtgataacga tcttgcgatc gacgatcaga gtatctcgag
     1741 tcaccacgca cgaatcgtca aggtacagtc ggtctatttt attgaagact tgaagagcac
     1801 aaatggcacg tccgtcaatg agaaacgaat cgagcgacat cagcttcgag atggggacat
     1861 catctcgatc ggtcaacacc gtctgatctt tcaagacatc agcgcggcag aaaccacgcc
     1921 cgccgccgta tccatagata tggataagac gatggtcctc gcaggtggga gtcaacttca
     1981 gggcctggcg gctccggccg cacgcctcct ggttacgtcg ggtaaaaccg atcagatgga
     2041 atacaccctg gccaaacagg ttgcactcat tggatctcaa caggatgcga cgattcgcct
     2101 gactgggtgg ttcgtgccca aagccgccgc acgggtcacc cggcgagggc ttcagtactc
     2161 gatcagtccg gcacagagga caaaaaaagt tctggtcaat ggaaaggaag tcgtcacaca
     2221 gcaggaactg aaaaacggcg accaaatcga ggtagcgggc gtcacgctct cgtttttcct
     2281 cacgccctct tcgaaaacgc agcgtcgata gcggtcagag actagtgttt gaagtacacc
     2341 gtacccttcg gcagagtctg atagcaccga tccgatacat cctgcgcatg atcttggagg
     2401 cactgtacca tgttgccgga ccctggcgtc atcgacatgc agtatcgcct gacgtcctcc
     2461 tgacaagccg cgatcatcct cgttcgctct tctctccact tgacgaatcg ttcccgtatc
     2521 agctgttgac atcgaggagc gagttgagac acgcgctgct caatgcattg acgacgcatg
     2581 ggaccatccg acgaggcggg acagaactgt tcaatttcag cctcgcactt gagtttagtg
     2641 acttgctgag accgaggatc gctggccatt cccgtagaga tgcccgcctg gggacgttga
     2701 gccggtgcac tctccttttg gacagccgac tggtcctgcg acggtagcgg cggcgacgtc
     2761 ggaatggtca gatcgagagt cggcactgtt gcaggcggtg gcaccgacgc tgctgcggca
     2821 cgaggagggt cgattgctgt gagttcctgc tgggaaggga agcttgagac gatcgtgccc
     2881 cagacaagcc ccagactcag agcggtcacc aaccataggt atactctgcc ggacgaccct
     2941 tgctgagtca tagctctgcc tccgctgaaa tcacctcagc ataggccaag cctgaataaa
     3001 aatcgatgaa atgtccggtt ttcgcgcgtg agtgagaagc gaggtgcgtc agaacaagac
     3061 gacgttcata cccggataga aaacgctaat tctcaggggt ttcgacaatg tgattttcga
     3121 atcgagtaca gccttccgcc aacaaacgat tagtcagcat ttctcccggc cattcgatgg
     3181 gcaaatcagc cgtaaacacc cacacatccg gcgaggtcca taccggaccg tgctcttcgg
     3241 gcaattcagg atcgaacagg tccgtccaga ccggcatgta cacaccgttt ccacattggg
     3301 tgtgaaggtg gacgatggtg cgagcccgca ccaagggatc caagtcacgc cacacagccg
     3361 ctgtttcatc ggccagccga tgaaggcgaa ccgcattttg cgcatcaaag agtgcgtagg
     3421 cggcatagac tggagcttcg atcgcgtccg gagcaaaggc taactccgga cattgttcga
     3481 ccaggccttc cgacaacgca cggcggtgtc ggtcctccat cgagtccggc aatccggcgt
     3541 ccggtgcacc aatcaattca aacaacagaa aggctgtatt ttcgacgggg ggatagagtc
     3601 gagcggcgat ggtctgcgcc cgctgggaat cagccacggc attcacgtgt tcattaactg
     3661 attgaagtgt caacggctcc agcgtcgcat ccgtgagcaa tcgcgattcc acccggacga
     3721 ctgttccagc cggaagacga agggcgcgat ggagcagatt cgccgtggcg acggggtcaa
     3781 tcttgagctt caacggatgg ctgagattcg cctggagcgg aagggctaac gccgccatga
     3841 cggcatacag cggcttctcg tctgcttcct catccagcaa caccgtgcac gcggattcag
     3901 ccagcagatc gaagagccat tccgccatat cctcatcaag cgcggcaagc accgtcagca
     3961 gatcgtgttc ccgcccctgt tcgataaacg cctggagcgt gtcaattgcc gcaccggtat
     4021 caccatggtc atggcggcgc gcattgatca gatgaatcaa gtcccgcgta aggggatccg
     4081 gacgtgacca gctcagcatg ttgcactcct gcaaaaccgg caacgcacta cacgaaccgg
     4141 tttgtgtcca gccaccatgt tgccaaactt tcgcccgagg agcaagcacc agataagttt
     4201 aattctcgtc ggtgatcttc gctgtcgact gttcatcatt caccgatcac cttgaccagc
     4261 acccgcttgc gtctgcgtcc atcgaattcc ccatagaaga tttgttccca cgggccgaaa
     4321 tccaagcggc ccttactaat cgccaccacg acttcccgcc ccatcaattg ccgtttgatg
     4381 tgggcgtcgg cgttgtcctc gccagtctcg ttgtggcgat aggaggctgc ttgcggagcg
     4441 atggcttcga gaaaccgctc gtaatcttgt aaaagtcccg gctcatcgtc gttgatatac
     4501 acgctcgccg tgatgtgcat ggcattgacg aggacgagtc cttcgacgac accgctcttg
     4561 cggacgacag tttccacctg aggagtgatg ttgacgaatg cacgccgtgt ctttgtttca
     4621 aaccagagtt cttctcggta ggatttcatt gccgtcaacc agtgggaatg gcattctgcc
     4681 caatgtaccc tgacggaagc aagcaagggg tcgtgcatct cgaccattgg tcagttccac
     4741 ggtcctagta tacggtataa tgggaaccat gcctcgacca ggcacgtccg gcaagggcgg
     4801 cccccccaag actccctctc tccgattatc cgccaatgcg ctcacggtgc tccgtcgacg
     4861 ttatttggcc aaatcaccga cgggaacagt catcgaaaaa ccggcgcagc tgctgtggcg
     4921 aaccgcttcg gacattgcgt tagccgaacg actttatccg gccaatggcc ggctgcccca
     4981 tgcagcccgg cgattctatg aattaatggc ccgacttgaa tttcttccca actcgccgac
     5041 cctcatgaac gccgggcggc gactgcaaca actctctgca tgcttcgtcc ttccggtcga
     5101 agactctttg gattccattt tcgaagccgt caaattccag gccctaattc atcaatcggg
     5161 aggcgggaca ggcttctcgt tcagtcacct ccgtccaaaa gacgatcgcg tcgcctcgac
     5221 cagcggagtt gcgtcaggcc ccgtctcctt catgcggatc ttcaatatgg ccacagacgt
     5281 catcaagcaa ggcggtacga gacggggggc caacatgggt gtgctgcggg tggaccatcc
     5341 agatattttg gagttcatcg ggatcaagca gaacctgaac gagatgacga atttcaacct
     5401 gtccgttggg attaccgatg cgtttatgca ggctgtcgaa cgcaaccgca cgtacgcgtt
     5461 gattaatccc catgacgggt caacggccca gcacctctct gctcgtatgg tctttgaccg
     5521 gcttgtcgag gcggcctggg caaccggtga gccgggcatg atctttctgg acacggtcaa
     5581 ccgagccaac ccaacgcctc aactcggcac gatcgagtcg accaacccct gcggcgaaca
     5641 accgttgctg gcccatgaat cctgcacgct cgggtcgatc aacgtcgcca ggttcgtctc
     5701 tccctcgaga actctcccca cggtggatta tgaaaggctg ggtgccacga ttgctctcgc
     5761 cgtccgcttt ttggacaatg tcatcgaccg aaaccactat cctctctccc aaatcgctgc
     5821 agcgacaagg cggacaagga aaattggact gggcatcatg ggattcgccg atcttctgat
     5881 cgccctgaac attcggtacg acagtccgga agctctgacg gtagcggaat cgttgatgaa
     5941 attttttcaa gctcgcgcac acgaggcttc ggcgcagctc gccgctgtgc gcgggacctt
     6001 ccccgcctat cgagccagcc ggctcagcac catgggtcag cgccgtcgca atgccaccgt
     6061 cacgacgatc gctcctaccg gcaccatcag catcatcgcc gattgctcgc ccggcattga
     6121 gccgctctat ggcgtgcgag tcgtccgcac cgtgatggag gacgttcgtc tgaccggctt
     6181 gcatccgatg tttgctcacc gcgcacgtgt gctcggcctc gatcttgcga aacttgaacg
     6241 agacgtggcg gcccacgaat ctattcaaca tctcacacag atccctgcgg gacttcgtcg
     6301 cctgttcgtc accgcacatg atgtggctcc cgagcagcac gtccgaatgc aggcggtgtt
     6361 ccaacgatac agcgatagcg gcgtgtcaaa aaccatcaac ttgccggcga cctccactca
     6421 ggcggatgtg gcttcggcct ttcgcttggc ttatcagctg gggtgcaagg gcttgaccgt
     6481 gttccgttca gggagccgag aaaaacaggt cctctcctgc acacagattc tggcttgttg
     6541 atccgtgtaa gtctcaaacc agccggcgaa atttccaaac gaaattgaca ccatcggggg
     6601 atggtgataa gtacttctcc aaattgccca taaaccagga tcttgcctcg tgcgctgagc
     6661 agggaggtat gcgatggcgg ccttcacaga ttgcttgact atgccaggga ccccttttca
     6721 atggagtctc ctgtttgctc gtcagccaga accggacttg gagttcaccg aagaggaact
     6781 tgaacaaaca acgaccacac gtccaatgtc cccgatgaaa ccgggcaaga agtccggcgg
     6841 aggacggccc atcatgtggg tgttactgct gattcttgtc ggcggaattg cttatgtcgc
     6901 catggaaccg gagatgctga cggagttgtt gaatcccatc ttaggtgaga acgctcccgt
     6961 agcaggaccg tcgccacgag tgatgactcc caagcctcaa cctccggcaa ccgcagcccc
     7021 cgttccggct ccaacaccag agccggcgcc ggctgtagca gctcctgcgc cgccggctcc
     7081 agccatgaca ccgcccggtg cagccccagc gccaactcca actgcacagc cagcagtcac
     7141 ggctattccc atgtttgccg aaggacagaa ggtcaccgtc acgtcagacc cgacggcgcc
     7201 aggggaaggt atcccgcttt ccacggattc ctcgggcgcg aaacccggcg caatcgtgaa
     7261 gccgggcgtg acgctcacgg tcttagatgg agaattacaa ggaaacagct gggtctattc
     7321 ggttcggacc gatgagggta tgaaagggtg ggtgccggag aaacggcttc gtttgaagtt
     7381 ctgatcctca catcccaatt ctttctcttc ttctctcgaa atcagcaggg cgttgacgat
     7441 tccgctctat gttataatcc gccgattcca cacctgaatt ttgttttcgg tggctatggg
     7501 tcaactggct gcggtgatct ttgacttcga cggtgtgatt gccgataccg aacccctcca
     7561 ctttgccgga tttcgccgga cgctggctga aattggtatc accctgaccg aatccgacta
     7621 ctacgcgaac tatttaggct atgacgaccg gggctgtttt aacgctgccc tggaggcacg
     7681 cggtcaccct accaatccca tcgtcatcag ccgtttgatg gaacggaaag ctcaggccta
     7741 tcttgaatcg attcgggaag atctgatcat tttccccggc gtccacgaat tggtacggga
     7801 ggcagcggct tcccatccat tagcgattgc ctccggggcc ctccggcacg agatcgaatt
     7861 gattcttgag caggcgggac tgcgaaaaca gttttcacac atcacaagcg cggaggacgt
     7921 gactcggggt aaaccggatc cgcaaccttt ctttcaggcg ctgcaaggtt taaatcggct
     7981 gcaacggggg cgggagattg ctcccgaagc ctgtttagtc atagaagatt cgattccggg
     8041 gattcgggca ggtaaggccg ccggcatgaa ggtgctggcc gtcgctaaca cccacactct
     8101 gcaggatctt cacgaagcgc atgccgtcgc ccacagcctc agcgaagtcc gtttgtctga
     8161 actacacgag cggttatggc ccgatcgatt gatggacccg aagaggcgat gactcctgca
     8221 gaagctcaag cctattgcac ggccttcacc aagaagagcg ggagcaactt ctactactcc
     8281 ttcctctttc tgcctcgtgc acgccggcac gcaatgtata cggtctatgc cttctgcaaa
     8341 gaggtagaca gtgccgttga tgaaccctca ccaggcagca atcctcgtga ggaactgcgc
     8401 cgatggcgga tggagcttga ggcggcctat catggcactc ccactttccc cgtcacgatc
     8461 agtctggccg agcatgctcg acgtctctcc atcccgcaag cctatttcga ggaattgatc
     8521 aaaggggtcg agatggacct gatgatgccg aactataaga cgtttcgcga cctttccctc
     8581 tattgttatc gggtagcgtc cgtggtggga ctgatctgtc tgcatgtgtt cggcaccacc
     8641 tcggcacggg cgcaagacta tgccgtcgac ctcggcatgg cgtttcaact caccaatatt
     8701 ctgcgtgatc ttgggaccga cgccgcccag gggcgtgtgt atctgcccga agaagatctg
     8761 gaaaagttcg gctgttcggc cgaaatgcta caacgccaac aagacagccc cccgttgcga
     8821 gaactcatcc gttttgaagc cgcgagggca catgcctact acgacaaagc ggctgccgca
     8881 ctggaagctt tgcccgccaa ggaccgccga gccctgacgg tcgcagaaat catgcgcgcc
     8941 gtgtatttca gaatgctgcg acgcattgaa caggccgagt atcatccttt tggtccccgc
     9001 gtacgactct cgaccacgag ccgactggct gtggcagccg gagtgtggct tcgctctcga
     9061 ttctcgtgac tcgtccaacc ccttcatcgg tcattgtcct gggcggcggc cttgccggct
     9121 taactgccgc ctatcgcctg gcttcccgcg gtactcatgt cacgctgctt gaactacgtt
     9181 ccacccttgg aggcaattca atcccgccgg gcaagggatg ggagccctcg cccctgacaa
     9241 tctccaatgg ccatcaagcc acctgggacc tcttgcgttc gctcggatca cacatcagtc
     9301 ctgctcactt ctcacacatt ccgctagagt tcctgatgcc agatgggacc acgaccacct
     9361 acccgtatac gcctctacct caacctgcac atgttgcagt gagtcttctt cgtttcaatg
     9421 ggctgacatg gcgagaacgc tggcgactgg tttcgtggct ggaacagatc tgggagggag
     9481 ctgttccact gccttcggat ctcgcccatc ggaccgctga tgaatggctg gctgcacttg
     9541 gacagggtga gtgtgcccgc cggaatgtgt gggatccgct ggctcggtgg ttgaccggca
     9601 atcagctgac acatcagtct gccgacacct ttcgtggggc tattgagcag accttttttc
     9661 gatcggcccg tgagagtcgt tgggcgatcg ttccgtcttt tcaggcattg ctggttcagc
     9721 cgatgatcga agcgctcaga acaagtggcg cgacagtttt gcttgatact gaagccactc
     9781 atttgctcag tgaaggcgat cgagtgaccg gagtcctcct caggaatgga tccacgctcc
     9841 aaggagactg gtatcttgcc gcactccccc ctcaacacct cgctaggctt ctacctgaac
     9901 gttggctctc acgttatgcc tacttccaac aactcgctga actgattact cgtcctacaa
     9961 tcagcgcaca aatcattatc tggcatgctc tcacgcagcc acgcatccta ctgttgagcg
    10021 gaggcttctt ctattctatg gttgcgcagc cgagcggatc gggtcacacg gtctgtcgat
    10081 tcttcggcgg agatgaagca ggagccgact caatgacttc agacacctta ctggcaactg
    10141 cggaagaggt tcttcgatcg ttacatatgc ttgctcctgg acaaatcgtt gagtcttgtg
    10201 actcccaaag aaatgaagaa ggatgcttgt ctctcgaacc aggtgtgcaa cttcgccgcc
    10261 ctcttcaacg cagtccgatt gccaatttcc tcgtcgccgg cgactggact gataccggat
    10321 ggccacccaa caataatgtg gagggcgcca tcgtgagcgc caaccgttgt gcggatatca
    10381 ttaccagttc gcctgcgtga ccgaactgac aacctggcat aggtacctga gccctaattg
    10441 ggaccgatca gagacaccag ccggtcggta tacgtgaagc tccagcgacc atgtaaaagc
    10501 gctgcgtgga cttggaacga gcaacaccgt acgcgacttt tttcttatgc gacaggaaat
    10561 cgagtccagt aaggtggaag tgctgagttg tcacttggag gaagatcgga aatagcactg
    10621 atcttcgaac cgatcgcctc gaagatattt agcaaccacg atccaggtac cgtgtgtatc
    10681 ccacagggcc acagaaatct tcccctcgac aacaaacggt tggagtgtga aggcggtgct
    10741 cag
//
LOCUS       DATDNW010000167         3742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_439353, whole genome shotgun sequence.
ACCESSION   DATDNW010000167 DATDNW010000000
VERSION     DATDNW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439353"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VI359_04135"
     CDS             complement(<1..341)
                     /locus_tag="VI359_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973474.1"
                     /translation="MRCVECFFENQSGAHRCRICGTTLQADTGPASSETLAIEATHHR
                     GGFFSFGTLITPACIQVAYVIGAAVITLAGLLMVALVLTGVAPEYTDANRDALLVGGL
                     TLLGIGNLLWRV"
     gene            1280..1987
                     /locus_tag="VI359_04140"
     CDS             1280..1987
                     /locus_tag="VI359_04140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HEY6973475.1"
                     /translation="MNNQVARRNHSFLRSRVTTTEVFCDFDGTITRVDATDAVLETFA
                     LPAWREWEDRWVKGEITSQECLARQVELIQADRDTLVQFTADLPIDEGIFALDRCCAQ
                     HGVPLTIVSDGIDLLVEAVLRRHGLLHLPVFSNHLRWDDHGSPTLSFPFAANNCESGA
                     GTCKCSLTRQSDSASTYRVYIGDGRSDQCVSAKMQTVFAKGKLRDWCECNGIQCIPFE
                     SLSDVSERLFPKEARIA"
     gene            1984..3375
                     /locus_tag="VI359_04145"
     CDS             1984..3375
                     /locus_tag="VI359_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY6973476.1"
                     /translation="MKPLPMIHISEQELREKEAKYCSHGDTVHYSPVPKFFQGCEGSF
                     LYDREDKPFLDLQMWYSAVNFGYRNKSIVEAVKSQLDQLPQLACQYLHEEKVLVAEKL
                     AKECLRAFDVEGRVQFNVGGAQAIEDSMKLVRNATGKSLFMAFMGGYHGRTLGASEIT
                     SSYRYRRRFGHFSNRAHFVPFPYCYRCPYGKKLDSCEYYCVNQVERLFETEYNSFWDS
                     KANEPEFVAFYVEPVQATGGYIIPPPEYFSRLKKILHDRKILLVDDEIQMGFFRTGKF
                     WAIEHFGVKPNVVVFGKAMTNGLNPISGLWAEESLISPQKFPPGSTHSTFSSNPLGTA
                     ASLATLQWIEQHNYEDTVAESGAYFLSQLKELKQRHATIGDVSGLGLALRIEVTTPDR
                     FTPDRALADRIFEAGLAGGIPSSRGPMGLVLDIGGHYKNVFTLAPSLDITRAEIDLGI
                     ELLDRLFTTCSEG"
     gene            3375..>3742
                     /locus_tag="VI359_04150"
     CDS             3375..>3742
                     /locus_tag="VI359_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6973477.1"
                     /translation="MRAHRLKRSGLLDDMVDRRKTDFALHTGSNVLVYLIHGVTGTPA
                     EMHYVGKELARHGWDVYATTLPGHCTRLRDLVKTSERDWRGHVEKQLAFIRDRYQYVF
                     VVGLSAGALLALDASTVVDV"
BASE COUNT          829 a         1014 c         1020 g          879 t
ORIGIN      
        1 actctccaga ggaggttgcc gatgccgagg agtgtcaacc ctccgacgag cagcgcatcc
       61 cggttcgcat ccgtatattc gggagctacg cccgtgagga ccagggccac catgagcaga
      121 ccggccagcg tgatgaccgc cgcgccaatg acgtaggcca cttggataca tgccggggtg
      181 atcagcgtcc cgaaggaaaa gaatcctcct cgatgatgtg tggcttcgat tgcgagggtc
      241 tccgatgaag caggtcctgt gtcagcctgg agggtggtgc cacagattct gcaccgatga
      301 gccccgcttt ggttctcaaa gaagcattcg acgcatcgca taccactgtt ctccctgata
      361 atcgcagcga cttgaaaaat gataggtcat ccctgttcct taaaaaaggg gataggtggc
      421 cgggaaagaa aaaggtgtta ggaaccggtt ccatattgac ccgagcgatc caagggaccc
      481 gcagaatcgc tccgaataga tcgtctatca tcttcagctc attcacggat tgagttcctg
      541 cgccgggtgc ccggtagaga ttgctgcaga catgacagct gttgcctgca aatatttccc
      601 gcatccttga cattgctcgg actagatgtt aactgtgaca acaagctgca agaaaaggat
      661 tcttctggtt aagaccctgc ctgagtccct gatcgcgtta aaggtgttat tttgtggagc
      721 atagcctact tcagcatcta agagcccttc gaccagttgg cggcatcgtc ctgatcctcg
      781 ccttccttct actgccgtat ctcattgacg tcgcctacta tggcgacttc accccaaccc
      841 attacgcaca agagaatatc aataatgagg aaggcacgca ctccgttctt gcgaaggctt
      901 ccctattgtt tgctgttgac caaacgaact ctggagccgg agttcgcttt ccagacccag
      961 atgtcacccc actaggcagt cttcaggcag gagcattcct cctgtccgag tacctctttt
     1021 tagactcact catttccagg cctcctccgc ttctctgatc tggttagttt cgtcatttta
     1081 gagacctgga gattgtctcc cgcggcattg cactgtgctc gcagagacct ctgccagaga
     1141 gtcttaggta aaagcactgt ttggaacttc tcttccgtac acggaaggtg aggggattat
     1201 cgtgagcggt tctcacaatg atccaatgca cgacttcggt caattgctcc atttttggaa
     1261 tggaaggaga aaagagttgt tgaacaatca agttgccagg cgcaaccaca gttttctgcg
     1321 cagtcgtgtg acgacgaccg aggtattttg tgacttcgat gggacgatca ctcgcgtcga
     1381 cgccacagac gctgtgctcg agacattcgc cttgccagcc tggcgagaat gggaagatcg
     1441 ctgggtgaaa ggcgagatca cgagccagga atgtctcgct cgccaggtgg agctgattca
     1501 ggccgaccgt gacactctcg tccaatttac cgctgatctt cccatcgacg agggcatttt
     1561 cgcgctagac cgttgttgtg ctcaacatgg tgttccactc acgattgtca gcgacggaat
     1621 tgatctcctt gttgaggcgg ttcttcgccg gcatggcctg ttgcatcttc cggtgttttc
     1681 gaaccacctc cgctgggatg accacgggtc tccaacactc agttttccct tcgccgcgaa
     1741 caattgtgaa agtggcgcgg ggacttgcaa atgctcgttg acccgtcaat cagattccgc
     1801 gtcgacgtac cgggtgtata tcggcgacgg ccggtcggat cagtgcgtct cagccaaaat
     1861 gcagaccgtg tttgccaagg gaaaactccg ggattggtgc gaatgcaacg gcatccaatg
     1921 catcccgttt gaatcgctgt ccgacgtatc cgaacgtcta tttccaaagg aggcccgcat
     1981 cgcatgaaac ccttgccaat gatccatatc agtgagcaag aactccgcga gaaagaagcg
     2041 aagtattgct cacatgggga caccgtccat tactcgccgg tccccaaatt ctttcagggc
     2101 tgcgaaggga gtttcctcta tgaccgagag gacaagccct ttctcgacct ccagatgtgg
     2161 tactcggcgg tcaattttgg ctatcggaac aaaagtattg tcgaagccgt caaatcgcag
     2221 ctggaccaac tgccccaatt ggcttgccag tatctgcatg aagaaaaggt gctcgtggca
     2281 gagaagctgg cgaaagaatg tttacgcgcg tttgacgtag aggggcgggt ccaattcaac
     2341 gtgggaggcg cacaggcaat tgaggactcg atgaagctcg tgcgcaatgc caccgggaag
     2401 agtctcttca tggccttcat gggtggctat catggccgaa cgttgggggc atccgaaatt
     2461 acttccagtt atcgctaccg ccgtcgcttt ggccacttct cgaatcgtgc gcacttcgtg
     2521 ccctttccct actgttaccg gtgcccctat ggaaagaagc tcgactcctg tgagtattac
     2581 tgcgtcaacc aggtcgagcg gttgtttgaa acagagtaca actccttctg ggactcgaag
     2641 gccaatgagc cggagttcgt cgcattctac gtcgagccag tccaagccac gggcgggtac
     2701 attatcccgc cgcccgagta tttttcacgg ctcaaaaaga ttctacacga ccgcaaaatt
     2761 ctcctggtcg atgatgaaat ccagatggga tttttccgga ccggtaaatt ttgggcgatc
     2821 gagcattttg gggtgaagcc gaatgtcgtc gtcttcggta aggccatgac aaatgggttg
     2881 aatcccatat ccgggctctg ggcggaggaa tcgcttatct ctcctcagaa attcccgcca
     2941 ggctcaacgc attccacctt ttcatccaat ccattgggca cagccgcctc gttagcgacg
     3001 ctgcagtgga tcgaacagca caattacgaa gacacagtcg cagaatcagg agcgtatttt
     3061 ctgagtcagc tgaaggagct caagcagcgt catgcgacga tcggagacgt gtcgggcctg
     3121 ggactggctc tccgcatcga ggtgaccacg cccgaccggt ttactcccga tcgagccctt
     3181 gccgaccgga tctttgaggc agggctcgcc ggggggattc catcatcccg tgggccgatg
     3241 ggattggttc tggacatcgg cgggcattac aagaatgtct tcaccctcgc gccttccctg
     3301 gatatcacca gagcagaaat tgatctcggg atcgaactgc ttgaccgctt attcaccaca
     3361 tgttctgagg ggtgatgaga gcacatcggc tgaaacgttc cggactgttg gacgacatgg
     3421 tggatcgccg taagacagac ttcgccctcc acacggggtc gaacgtgttg gtctatctga
     3481 ttcatggtgt caccggtacg ccggctgaaa tgcactacgt ggggaaggaa ctcgcgcggc
     3541 acggatggga tgtctatgcc accacgttgc ccgggcactg tacaagactt cgggatcttg
     3601 tgaagacgag tgagcgcgac tggcgtggtc atgtggagaa gcaactcgcc ttcatacgag
     3661 atcgctacca gtatgttttt gtcgttgggc tcagtgcagg cgcgctcctg gcgttagacg
     3721 catccaccgt cgtggacgtg aa
//
LOCUS       DATDNW010000168         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_18745,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000168 DATDNW010000000
VERSION     DATDNW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18745"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            578..934
                     /locus_tag="VI359_04155"
     CDS             578..934
                     /locus_tag="VI359_04155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973478.1"
                     /translation="MRSELRQQPRLRIPAPFVCVFSRIGLTRWLSADRDGLGVVLDVS
                     LKGARVMSAVTMTPGDQLAVSLRLPNQISTMNTDATVRWGSNQTFGLEFATLSRTAEM
                     RLRKYLTQTSSTQLTA"
     gene            complement(1035..>2445)
                     /locus_tag="VI359_04160"
     CDS             complement(1035..>2445)
                     /locus_tag="VI359_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6973479.1"
                     /translation="IKRTFPGLNIRFAVSLRRDIVQFLNTATGQADAGGRKLDENVSD
                     ILGELTSEAQAEAMEDASAGGGLDENDSAIVRLGNQIIADAYRQGASDIHIEPYGDKR
                     ETLVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMANLDIAERRKPQDGKIKFKLGGDRE
                     IELRVATIPTAGYNEDVVMRILAASEPLPLDKMGFSDRNLKILKQIAEKPYGIILCVG
                     PTGSGKTTTLHSVLGYINTPDIKIWTAEDPVEITQYGLRQVQVQPKISFTFAAAMRAF
                     LRADPDVIMVGEMRDKETADIGIEASLTGHLVLSTLHTNSAVETVTRLLDMGCDSFSF
                     ADAMLGVLAQRLSRRICKDCKEQYVGSKEEYEELRQGYGPEYWDKLGIKQDNTFRLSR
                     GKGCETCNRSGYKGRVALHELLEGTERMKKMVQSKARTEEMMKVALEEGMTTLVQDGI
                     QKVLQGLTTYKEVKAVAIK"
BASE COUNT          504 a          678 c          658 g          605 t
ORIGIN      
        1 aacatctgct gtgcgaacac ttcaggggta aagcctttat cgcctcgggt tttgtcatta
       61 atcagagact ggttcagagc gatagtggtt cgcccagagt ccaagaggat ggcgataagg
      121 cggccggttt cgatgctttc actctcggtg ctcgaactcg atacggttgg aacaaggccc
      181 ccgtaaacaa tcgtcagaag cgagataatg agagcccacc ctctcctcca catcataatc
      241 ctccctaccg gtgcgacaca gatctgtgga tgctgcttgc ccgcacaccc tggcatcata
      301 atggcctcaa gtctcacaaa atttctactc ccctaagagg ggggtaaccc gggaatcagg
      361 gagcgtgtta acgagccggt aagctcttct cgtacaggag aagacaggga gtatgtccgc
      421 tttcctcaaa tttcgtctct tgccaatcat cagtggtaac tggacgggca gcaactcggg
      481 gcaacttttt tgaaatgtgt tcgtgaacag atgaagctag cgtatggtac aggactgaca
      541 cacgtggtgc tctgaaatga gatcaattca ggaacccatg cggagcgaac tgcgacaaca
      601 gccacgcttg cgtatccctg ccccatttgt gtgtgtgttc tcaaggattg gactaacccg
      661 ctggctttct gctgatcgcg acggtctcgg cgttgttctg gacgtttccc tgaagggcgc
      721 gcgggtgatg agtgccgtca cgatgacgcc cggtgatcaa ctcgccgtca gtctgcggct
      781 gcccaatcag atttcgacca tgaataccga tgcgactgtc cggtggggga gtaaccaaac
      841 cttcgggttg gaattcgcga cattatcccg tactgccgag atgcgactcc gcaaatacct
      901 cacgcagacc tcttccactc agctcacagc gtaaacatcc cgaaaagtat ctggtcatcg
      961 gaggattgag gcttacaagc atgcccaggc ttgagtccgg taagacagcc ggcggaagat
     1021 atctccgccg ggggctactt gatcgcgacg gctttcactt ctttataggt cgtaagacct
     1081 tgaagcacct tttggatgcc gtcctgaacg agggtcgtca tgccttcttc gagcgcgact
     1141 ttcatcatct cttccgttcg agccttggat tggaccatct tcttcatccg ctcggtccct
     1201 tccaagagtt catgcaatgc gacccgcccc ttgtatcctg aacggttgca ggtctcgcaa
     1261 ccttttccgc gcgacaagcg gaacgtatta tcttgcttga tgcccagctt atcccagtat
     1321 tcgggaccat acccctgccg cagttcttcg tattcttcct tgctgccgac gtactgttcc
     1381 ttgcagtcct tgcaaatccg tcgagacaaa cgctgggcga gcacgccgag cattgcgtcc
     1441 gcaaaactga aggagtcgca tcccatgtcg agcagccggg tgaccgtttc caccgcgctg
     1501 ttggtgtgaa gggtgctcaa aactaggtgg ccggtgagcg aggcttcgat gccgatgtcg
     1561 gccgtttctt tgtcgcgcat ctcaccgacc atgatcacgt cagggtcggc acggagaaag
     1621 gcacgcatgg ctgcagcaaa cgtgaagctg atcttcggtt ggacctggac ctgacggagc
     1681 ccatattgcg tgatttcgac cggatcttca gccgtccaaa ttttgatgtc cggagtgttg
     1741 atatatccga gaaccgagtg gagcgtggtc gttttccctg atcccgtcgg acccacacag
     1801 agaatgatcc cgtacggttt ttcggcgatc tgcttgagga ttttcaggtt ccgatcggaa
     1861 aatcccatct tgtccagcgg cagaggttcg ctggctgcca ggatacgcat cacgacatct
     1921 tcgttatatc cggcggttgg gatggtcgcg acgcgcaact cgatttctcg atcgcccccc
     1981 aatttgaatt tgatcttgcc gtcttgaggt ttgcgtcgtt ccgcgatgtc gagattggcc
     2041 atgatcttta atcgcgagac gatggcgcga cgatagctct gcggaatctt catgtattcg
     2101 aaacaatcgc cgtctacgcg gaatcgcacg agcgtttctc gcttgtcccc atagggctca
     2161 atgtgaatgt cagacgcccc ctgccgatag gcgtcggcga tgatttggtt tcccaaccgt
     2221 acgattgcac tgtcattttc atcaagcccg ccgcctgctg aggcgtcttc catggcctcg
     2281 gcttgggctt cgctggtcaa ctccccgaga atgtcagata cgttttcgtc cagttttcgt
     2341 ccgcctgcat ctgcttggcc agtggcggta ttgagaaatt ggacgatgtc gcgacgaagg
     2401 cttacggcga accggatgtt gagaccggga aacgttcgct tgatg
//
LOCUS       DATDNW010000169         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_439569, whole genome shotgun sequence.
ACCESSION   DATDNW010000169 DATDNW010000000
VERSION     DATDNW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439569"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VI359_04165"
     CDS             <1..417
                     /locus_tag="VI359_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY6973480.1"
                     /translation="NWARKSALWPMTFGLACCAIEMIASVSSRYDLDRFGAGVFRASP
                     RQSDLMIVAGTVTRKMAPVIRRIYDQMPEPRYVISMGSCATSGNHYNSYAVVQGVDQI
                     VPVDVYVPGCPPRPEALMDGLLKLQEKIQREKVFVK"
     gene            417..2171
                     /gene="nuoD"
                     /locus_tag="VI359_04170"
     CDS             417..2171
                     /gene="nuoD"
                     /locus_tag="VI359_04170"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY6973481.1"
                     /translation="MNQTLAETLLKKFPDAVRALEVDTARGEVSVSIHASHLLDVARY
                     LHDAPEASFNHLTDICSVDYPEDQERFEVVYQLHSLPLRQRLRVKARIPEDHPTIESV
                     TSIWKGAEFLEREVYDMMGITFTGHPDLRRILMPEDYAEGYPLRKDFPAEGRGWRSHF
                     DFIPRLDEPTMTTTTDELSEEQRKPFLAETGSTGSRRREELLLNMGPQHPATHGVLRV
                     VLELDGERIVKATPDLGYLHRGVEKLSEGLAYMQIIPHTDRLDYVCAMANNYAYVRAV
                     EKLLTITVPERAEYIRTIVAEMQRIVGHLFWLGTQALDIGAMTVFFWTFRERETLLDM
                     FEKLCGARLTLNYYRIGGVDSDFTDELVQRLKAFLDTFPEKVREYDSLIASNRIWLGR
                     TRNVAVISAEDAINFSCTGPVLRGSGVAYDVRKFEPYGAYDKVEWEVPIGKNGDTYDR
                     YWIRMEEMRQSARIIKQCLEQMPPGPIMADVPQYIPPPKPHVMRDMESLIHHFIIFTQ
                     GFKPPKGETYCGTEAPKGELGFFIVSDGSPRPYRLKIRSPSFVHMGAFDHMARGYLIS
                     DIITIFGTYDIVMGECDR"
     gene            2328..>2584
                     /locus_tag="VI359_04175"
     CDS             2328..>2584
                     /locus_tag="VI359_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY6973482.1"
                     /translation="MLKEKYQKEIDEIVSRYPVRRSALIPLLYLAQREQGFVTEAAMK
                     EIAVLLRLTPPQVYETVTFYTMLNLKKVGKFHIQICKSLMCA"
BASE COUNT          546 a          721 c          760 g          557 t
ORIGIN      
        1 aattgggctc ggaagtcggc cttgtggccg atgacgttcg gcctggcttg ctgcgcgatc
       61 gagatgatcg cgagcgtctc gtcgcggtac gacctcgacc ggttcggcgc cggggtgttc
      121 cgcgcgtcgc cgcggcaatc agacctcatg atcgtcgccg gcaccgttac gcgcaaaatg
      181 gcgccggtga ttcgccgcat ctatgaccaa atgccggaac cgcgctatgt gatctcgatg
      241 ggttcctgcg ccacctccgg caatcactac aacagctacg ctgtcgtcca gggtgtggat
      301 cagattgttc cggtggatgt ctatgtgccg ggctgcccgc cgagaccgga agcgctcatg
      361 gatggattgt tgaagctaca ggaaaagatt caacgtgaaa aagtcttcgt gaagtaatga
      421 accaaactct ggccgaaacc ctgctcaaga agtttccgga tgcggtgcgt gcgctcgagg
      481 tcgacacggc acgcggcgaa gtctctgttt cgattcacgc ctcgcacctg ctggacgtcg
      541 cccgttatct ccacgatgcc ccggaagcgt cgtttaacca tctcaccgac atttgctcgg
      601 tcgattatcc ggaggaccag gagcggttcg aggtcgtcta ccaacttcat tcgttgccgc
      661 ttcgtcagcg ccttcgcgtg aaggcacgga ttcccgaaga tcatccaacg atcgaatcgg
      721 tcacgagcat ctggaagggg gccgagtttc tcgagcgcga agtctacgac atgatgggca
      781 ttacatttac cggtcatccg gacctccgtc gcatcctcat gcctgaagat tacgcagagg
      841 gttatccgct ccgaaaagat tttcctgcgg aaggtcgcgg atggcgcagc cacttcgact
      901 ttattccgcg actggacgag ccgacgatga ccacgaccac tgatgaactt tccgaagagc
      961 agaggaaacc gttcctcgcc gagaccggat ccaccggttc ccgccggcgt gaagaactct
     1021 tgctgaacat gggaccacag cacccagcca cccatggagt tctccgcgtt gtgctcgagt
     1081 tggatggcga acgaatcgtg aaagcgaccc ccgacctggg ctatctccat cgcggcgtgg
     1141 aaaaactctc cgagggcttg gcctacatgc agatcatccc ccacacggat cgtctcgatt
     1201 acgtctgcgc catggccaat aattacgcct atgtacgggc tgtcgaaaaa ctcctgacca
     1261 tcaccgttcc tgaacgggct gaatacatcc ggaccatcgt ggctgagatg cagcgcatcg
     1321 tcggtcacct gttctggctt ggaacccagg ccttggacat cggtgccatg acggtttttt
     1381 tctggacgtt ccgcgagcgc gaaacgttgc tcgacatgtt cgaaaaactc tgtggcgccc
     1441 gcttgactct gaactactac cgcatcggcg gtgtcgacag tgatttcaca gatgaactcg
     1501 tgcaacggct caaggctttt ctcgacacgt tccctgagaa ggttcgagaa tacgactcgc
     1561 tcattgcctc gaatcgcatt tggctcggac gcacgaggaa tgtcgcggtc atttcggcgg
     1621 aagatgcgat taatttcagt tgcaccgggc cggtcttgcg cggatcgggc gtcgcttacg
     1681 atgtccgaaa gtttgagccc tacggcgcct acgataaggt cgaatgggaa gtgcccatcg
     1741 gtaagaatgg cgacacgtat gaccggtact ggatccgcat ggaagagatg cgccagagtg
     1801 cgcggatcat caagcagtgt ctcgaacaga tgccgccggg tccgatcatg gctgatgtcc
     1861 cccaatacat tccgccgccg aagccgcacg tcatgcggga catggaaagc ctgattcatc
     1921 actttattat ttttacgcag ggcttcaagc cgccgaaggg ggagacatac tgcggcacgg
     1981 aagcgccgaa aggcgaactg ggctttttca tcgtgagcga cggaagtccc cgtccctacc
     2041 ggctgaagat tcgctcacca tcgttcgttc acatgggggc atttgaccat atggcgcgag
     2101 gctatttgat ttcggacatc atcacgatct tcgggacgta cgacatcgtg atgggagagt
     2161 gcgatcgatg aggcctagga gccaggtgtt cgcactgctc gcggaacgcg cgccttagaa
     2221 ggcctcgctt gggacacggg cgcgtcttgg cgcgaccagg gtgggcgggt gagatcaggc
     2281 gcgcagtcgg cccaatcacc ccgtcgcctc actgttgggg aagtggaatg ttgaaagaga
     2341 agtatcagaa agaaatcgac gagattgtga gccgctatcc ggtgagacgg tcggcgttga
     2401 ttccgctgtt gtatctggct caacgggagc aggggttcgt cacggaggcg gcaatgaagg
     2461 agattgcggt attgctgcgc ctgacaccgc cgcaagtcta tgagaccgtc actttctata
     2521 cgatgttgaa tctcaagaag gtggggaagt tccacatcca gatttgcaaa tcgttgatgt
     2581 gtgc
//
LOCUS       DATDNW010000170         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_18791,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000170 DATDNW010000000
VERSION     DATDNW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18791"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VI359_04180"
     CDS             <1..636
                     /locus_tag="VI359_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6973483.1"
                     /translation="DHPIVLQGCRQLLEDAGVEQIVQAQSLADGFRLYRTQKPDVVIV
                     DLAMRTGALGGLSFIRRLRLHDAKTPILVFTMHSDPVIVSRALEVGATGYVLKDTSPD
                     EVVKAFQRVRENRPYLSHDLASEVAFMEARGTTNPLRRMTVRELQTLALVAEGKPYGV
                     IAEHLHVSYKTVANTCTQLKAKLGVRTLPELMRIAIQHLPTASAKTPREWS"
     gene            1230..>2452
                     /locus_tag="VI359_04185"
     CDS             1230..>2452
                     /locus_tag="VI359_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY6973484.1"
                     /translation="MNMKFGVLAGAIVAVTMGLSGQAMAGSDEEQLARMKDPDQWPAP
                     GRDFSLTRHSPLADINTKNVSKLQMIWLQGTNALRGHEGQPLVVKDVGGKTVLFMVSG
                     CPAMSNCNVVQALDLSDPDHPKQIWNYVKKTDRDESAVPRACCDTVNRGGSYADGKFV
                     YGTLDGFLIALDGQTGKEVWVVKLAYPEHGETITPAPLIADDKVIEGFGGDEFAARGR
                     MTAYNLKDGSVAWVCQSTGSDKDVCLTADTNKANPQYGTAGSDLGIKTFPGEDYKIGG
                     GAAWGWYSYDPDLKLVYYSTGNPGLWSPAYRCPDKSHEECNAGQFDNKWSMTIFARKV
                     DTGEAVFAYQMTPFDQWDYDGINENMLTDMDIDGKKVKALTHFDRNGFAYVLDRTDGT
                     LLRATKYVTVDWAEK"
BASE COUNT          514 a          707 c          730 g          501 t
ORIGIN      
        1 gatcatccga tcgtgctgca aggctgtcgc cagctgctcg aggatgccgg cgtcgagcag
       61 atcgtccagg cgcagagtct cgccgacggc tttcgccttt atcgtacgca gaagcccgac
      121 gtggtcatcg tcgatctcgc catgcgcacg ggagctctcg gggggctgtc cttcatcagg
      181 aggctgcgcc tgcacgatgc gaagacgccg atcctcgttt tcaccatgca ctccgacccc
      241 gtgatcgtca gccgcgcact cgaggtcggc gcaaccggct atgtgctcaa ggacacctct
      301 cccgacgagg tggttaaagc cttccagcgg gtccgcgaga accggcccta tctcagccac
      361 gacctcgcct ctgaggtggc cttcatggag gcgaggggca ccaccaatcc cttgcggcgc
      421 atgaccgtgc gcgagctgca aacgctcgct ctcgtcgccg agggcaaacc ttacggcgtg
      481 atcgccgaac atctccatgt aagctacaag actgtcgcca atacttgcac ccagttgaag
      541 gcgaagcttg gcgtgcgcac gcttcctgaa ttgatgcgca ttgccatcca acaccttccc
      601 acggcgagcg cgaaaacgcc gcgcgaatgg tcgtagcgaa gccaatcgct cccgaatgaa
      661 tcgcatctcg cgtaaagaga gatgtgactc gtgcactgac ggagcgtttc cctcataaag
      721 ttaagagggg ggatgaggct aaaggtgcgc cgcacacaaa tcgtggaaga tttcgcttga
      781 cactatagtc tgccgggcac gcgttcttga tgcagtgcag cgaaacgcga tgcgcgattg
      841 gcacaaatta ttgccgcgag cacttttcac ttgcaaagcc attacatttg gcaattatga
      901 gcgggcagcg tagcaagggg tgggatgctg gtttccatcg ataattcgat atctccggcg
      961 tcgagctctg agttaggctg caattcctcg tgaggtgtca ccgcgtgatg tgttcatcgg
     1021 aggacccgcc gccaggagcg ggcgcgaggc ggtttttttt gcttctgcgc attcgggatt
     1081 gcctcccata tccacagtga caactgcaaa acgcaagggc tcggtgctct cttggcattc
     1141 gcgcctaggg agaaacgtac cccctgcaca tttagagaag aactcagctt tcgttcggtg
     1201 aactgagtag ctagggagag gagaaagaca tgaatatgaa gtttggagtt ctggctggcg
     1261 ccatcgtcgc ggtgacgatg ggtctgtccg gccaggcgat ggcaggctcc gatgaggagc
     1321 agcttgccag aatgaaggac cccgaccagt ggcctgcacc tggtcgcgat ttcagcctca
     1381 cgcgccatag cccgctggct gacatcaaca caaagaacgt cagcaagttg cagatgattt
     1441 ggcttcaggg caccaacgcc ctccgtggtc atgagggtca gcccctggtg gtcaaggatg
     1501 ttggcggcaa gaccgtgctg ttcatggtct ctggctgtcc cgccatgtca aactgcaacg
     1561 tggtccaggc tcttgacctg agcgaccctg atcaccccaa gcagatctgg aactacgtga
     1621 agaagaccga tcgtgacgag tctgctgttc cgcgggcttg ctgcgacacg gtcaatcgcg
     1681 gcggttccta tgccgatggc aagttcgtct atggcactct cgacggtttc ctgattgccc
     1741 tcgacggcca gaccggcaag gaagtctggg ttgtcaagct cgcctatcct gagcatggcg
     1801 agaccatcac ccccgctcca ttgatcgccg acgataaggt gatcgaaggc ttcggtggcg
     1861 acgagttcgc agctcgcggt cgcatgacgg cttacaacct caaagacggc tcggtggctt
     1921 gggtttgcca gagcacgggt tccgataagg acgtgtgcct gacggccgac accaacaaag
     1981 ccaatccgca atacggcact gccggttccg acctcggtat caagactttc ccgggcgagg
     2041 actacaagat cggcggcggc gcggcttggg gttggtatag ctatgatccg gacctgaagc
     2101 tggtctacta ctcgacgggt aaccccggcc tgtggagccc ggcctatcgc tgcccggaca
     2161 agagccatga ggagtgcaac gctggccagt tcgacaacaa gtggtcgatg acgatcttcg
     2221 cccgcaaggt cgacaccggt gaggcagtct ttgcctatca gatgaccccg tttgatcagt
     2281 gggactatga tggcattaac gagaatatgc tcactgacat ggacatcgat gggaagaagg
     2341 tgaaagccct gacccacttc gatcgtaacg gttttgccta tgtgctcgat cgcaccgacg
     2401 gcactctgct ccgcgccacg aagtacgtga cggtggactg ggccgagaag at
//
LOCUS       DATDNW010000171         6532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_18831,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000171 DATDNW010000000
VERSION     DATDNW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18831"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="radC"
                     /locus_tag="VI359_04190"
     CDS             <1..670
                     /gene="radC"
                     /locus_tag="VI359_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY6973485.1"
                     /translation="LAQGPTSLTDAQLLAILLRVGRRRSSALQVGLEVLDRLNGLSGL
                     AHCGIEELCAIPGVGAAKAAQLKAALELGKRALAAPLAKGTKITSSRDLFHHYHPSLR
                     DLRHEIFKVVLLDAKHAIMRDATVSEGSLTLSIVHPREVFNLAVRESAAAVIFLHNHP
                     SGDPTPSQEDRVLTTRLVAAGDVLGIRVLDHLIVGDGRYVSFADHGWLTCSAAGTEIQ
                     KEWC"
     gene            735..2834
                     /gene="fusA"
                     /locus_tag="VI359_04195"
     CDS             735..2834
                     /gene="fusA"
                     /locus_tag="VI359_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY6973486.1"
                     /translation="MNELRAESLRNVAVVSSAGAGKTSLVEAILFTTGTIPVLGAVAQ
                     GNTASDFEPEEIHRRTSISTSLLRCSWQHTILNLIDPPGALNLLGEPLMALQAVDAVI
                     IVVGTSGVRSELAKVWARVKELRLPCLIFANEFDKENVSLEDLRALCHRSFDATVLPL
                     SLPINTSGHLVGVIDLLAQVQVHSQAESAKVQQTPIPESLQQEIGEARKRLQEAIAEV
                     DDRLLEQYCATGELGQEDMLNGLRSAVQTGQLVPLFCGSALKNVGIVPLLNAITSLLP
                     SPAERAERAPLEAVNPDTGESISRQGREGDPFSAIVFKTIIDPFIGRLSYVRVLSGTL
                     QADSVVFNATRALKEKGGHLYTLIGKKHTAVPFAKAGEIVALGKLKDTQTGDTLCEET
                     APIRYASVTLPKPVMSFAIEPKAKADVDKVSLGLHKLIEEDPTLEFHRNADTKEMVLS
                     GLGQLHIDIALEKLHRKYGADVVLHTPKIPYRETIRGTAQAQGKYKKQTGGHGQYGDC
                     WLELAPLPRGAGFTFANKIVGGAIPRNFIPAVEKGVIEAMQHGTLAGCPVVDLSVTVY
                     DGSYHVVDSSEMSFKIAASMGFKKAMESAHPVLLEPLMTLEVEAPADHIGAVIGDLNS
                     RRGRILHVEAKDHTELITALVPLAEVLTYATTLNSLTGGRGSYGMGFTRYEEVPRDLA
                     TRVIEEQKAEKHTVVAQ"
     gene            complement(2841..4223)
                     /locus_tag="VI359_04200"
     CDS             complement(2841..4223)
                     /locus_tag="VI359_04200"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY6973487.1"
                     /translation="MARYIAPLLSVTLLTLLDPVAMAGAEPDTTPARSGAGLQMLEEI
                     QTVITELAESAKPSVVNLFPLSGPGRAREMPQERIPNSPGSGSGLIVDAEGHIVTNNH
                     VIGDATEIEVRLSDKSTLIGQVIGKDPDTDLAIIKVTPDHPLAYAKFGDSSKVRVGQW
                     VLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLFNLHGEV
                     IGINTAIINFAQGIGFASPANMAKQVIQQLLAKGRVVRGWLGVGIQPLTPELARKFGV
                     REGEGVLVNEVFEKDPAASAGIKPGDIITKIDGNLVDTPNRLSRLVAGLLPGATAKVE
                     VVRDQVRLTLEVGLTERRDAAVVTSLPQARTEVRLGLDVQDLTAGLAEKFKLRESKGV
                     LITKVDPGSLAQSEGLREGDLIKEVNRAEVGTVGEFSTAIAKIRRGETVLLRVLRENR
                     AFYVVLKSSE"
     gene            complement(4284..4913)
                     /locus_tag="VI359_04205"
     CDS             complement(4284..4913)
                     /locus_tag="VI359_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY6973488.1"
                     /translation="MFLEFLGRLQKELSITGQAIYETIIAVAERVNRKVEVLRLHGQA
                     SAVITQVDIAHAELGRQITALWTRRYLPDHEASVATELNQLVVQTTARVHQLKQMLLT
                     VDSQVRALKLETTQEDLLTLQRDLSRRSAALERIVLSPGAPAIGRQIGELRLPESVRV
                     VTIFRGPFFINSSEDLVLRSEDVLLVVGLQTDLAHAVAQLTVADRVRSA"
     gene            complement(5244..5648)
                     /locus_tag="VI359_04210"
     CDS             complement(5244..5648)
                     /locus_tag="VI359_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973489.1"
                     /translation="MTGVETLAWLVLAFADGSDCLLQGTFQWLPGGRAFVREDGCAVQ
                     SHQEGGYVVFWSENRWVAIKIPTRAKSLSYRWGRAMAFVNGDSVKVQHGKIIGALQSG
                     PLMSDHDNTTTLSKWRIGDVLVHRRVSHHQIM"
     gene            complement(5767..>6532)
                     /gene="lipA"
                     /locus_tag="VI359_04215"
     CDS             complement(5767..>6532)
                     /gene="lipA"
                     /locus_tag="VI359_04215"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY6973490.1"
                     /translation="ARCPNVWECWNARTATFLILGDICTRRCHYCSVTTGRPMELDRE
                     EPLRVAHAVQALGLRHAVITSVNRDELEDGGASVFAETIRYIRKLIPTCTIEVLIPDF
                     EGNNAALATVCAEKPDILNHNIETVQRLFPTVRPQGKYQRSIALLGRAKELGMTTKSG
                     LILGMGETMDEAREVMRNLRSIDCDIMTIGQYLQPTKDHLPVARFYHPDEFVALEEEG
                     HALGFIHVESGPLVRSSYHAERQVASSPARVSPCPH"
BASE COUNT         1433 a         1859 c         1795 g         1445 t
ORIGIN      
        1 gcttgcgcaa gggccaactt cgctaaccga tgcacaactc ttggccattc ttcttcgagt
       61 cggtcgccgc cgatcctcgg cgctacaagt tgggcttgaa gtgttagacc gtctcaacgg
      121 actttcggga ctcgcacatt gtgggatcga agaactctgt gccattccgg gagtcggcgc
      181 cgcaaaagca gcacaactga aagcggcact cgagctcggt aaacgtgcgc tcgccgctcc
      241 attggccaaa ggcacaaaga ttacgtcgag tcgtgacctt tttcatcact accatcccag
      301 cctacgagac ctccgccacg agattttcaa ggttgtgctc ttagacgcca agcatgcgat
      361 tatgcgggat gcgaccgtat cggaaggcag cctgacgctc agcattgttc atccgcgtga
      421 agtattcaat cttgccgtgc gagaatcagc cgctgccgta atttttcttc ataaccatcc
      481 cagtggcgat cccacaccga gccaggaaga tcgtgtcttg acaacccgac ttgtggcggc
      541 gggcgatgtg ctggggattc gagtcctcga tcacctcatt gtgggtgacg gccgctatgt
      601 gagttttgcc gatcacgggt ggctaacatg ttcggccgca ggcactgaga tacagaagga
      661 gtggtgttga tgtaatccat ctatgcaaca gccggaagaa tcccttgcct tccggtgtga
      721 caaaggagga tcccatgaat gagctacgtg cggagtcact cagaaatgtg gctgtcgtct
      781 cgagtgccgg ggccgggaaa acctcgcttg ttgaagctat cctctttacc accgggacca
      841 ttccggtgct cggggctgtg gcgcaaggga acacggcctc cgatttcgaa ccggaagaga
      901 ttcaccgacg gacttccatc agtaccagcc tccttcgctg ttcttggcaa cacaccattc
      961 tcaatctgat cgatccacct ggggcgctca accttctcgg cgagccgttg atggcactgc
     1021 aggcagtcga tgcggtgatc atcgtcgtcg ggacctccgg ggtccggagc gaactcgcga
     1081 aagtctgggc tcgcgtcaag gagttgcgac tgccgtgtct catctttgcg aacgagttcg
     1141 acaaggaaaa tgtctcgctc gaggacctca gggccctgtg ccaccgcagt tttgacgcga
     1201 cggttcttcc gctctcatta ccgatcaaca cgagtggcca tctcgtcgga gtgattgatc
     1261 tgttagccca ggttcaggtc cactctcaag cagagtccgc caaagttcaa cagactccga
     1321 ttcctgaatc gctgcaacag gagatagggg aggcgcgcaa acggctccag gaggcgattg
     1381 cggaagtgga cgaccgattg ttggaacagt actgtgcgac cggagaactc ggccaggagg
     1441 atatgctgaa cgggctccgg tctgcggtac agactggtca gctggttccg ctgttctgtg
     1501 ggtccgcact caaaaatgtc ggcatcgtcc cgctgctcaa tgccatcacc agcctgctgc
     1561 cgtctccggc agagcgggca gagcgagccc ccttggaggc cgtcaatcca gacaccgggg
     1621 aatcgattag tcgccaaggg agggaagggg atcccttttc cgccatcgtg ttcaaaacga
     1681 ttattgatcc gttcatcgga cggctctcct atgtccgggt actttccggt accttacagg
     1741 ccgactccgt tgtatttaac gcaacacgtg ctttgaagga aaaaggggga catctttata
     1801 cccttatagg caagaaacat acggccgtcc cgtttgccaa ggccggagaa atcgtcgcgc
     1861 tgggaaaact caaagatact caaaccgggg atacgctgtg cgaggagacg gctcccatcc
     1921 gctatgccag cgtgactcta cccaagccgg tcatgtcgtt tgccattgaa ccgaaggcca
     1981 aagcggacgt cgacaaggtc agtttgggtc tccacaagct gattgaagaa gatccgacgc
     2041 tcgaattcca tcgtaacgcg gacacgaaag aaatggtgct gagcgggtta gggcaactcc
     2101 acatcgacat cgcattggaa aaactccatc gcaagtacgg ggcggacgtc gtcctccaca
     2161 cgccaaaaat tccctatcgg gaaacgattc ggggaacggc gcaagcgcaa gggaaatata
     2221 aaaaacagac aggcggccac gggcaatatg gcgactgttg gctcgaactc gctccgcttc
     2281 cgcgcggagc tggctttacg tttgccaata aaattgtggg cggcgcaatt cctcgcaact
     2341 ttattcccgc cgtggaaaag ggcgtgattg aggcgatgca gcacggcacg ctcgcaggct
     2401 gtcccgtcgt cgatctctcg gtcactgtct atgacgggtc ctaccatgtc gtggattcgt
     2461 cggaaatgtc gttcaagatt gccgcctcga tgggattcaa gaaagcgatg gaatcggctc
     2521 atccggtgct gctggaacca ttgatgacgt tggaggtcga ggcgccggcg gatcacatcg
     2581 gcgccgtgat cggagacttg aattcgaggc ggggacggat cctccacgtc gaagcgaagg
     2641 atcatacaga actcattacc gccttggtgc cattagctga ggtgttgacc tatgcgacca
     2701 cactgaattc gctgactgga ggacgcggca gttatggaat ggggtttacc cgctatgagg
     2761 aagtcccgcg ggatctggca actcgagtga ttgaggaaca gaaggcggag aaacatacgg
     2821 tcgtcgcgca gtgagcatca ctactcagac gacttaagga ccacgtagaa tgcgcgattt
     2881 tctctaagaa cccgtagcag gactgtctct ccgcgtcgga ttttcgcgat cgcggttgag
     2941 aattcgccga ccgtgccgac ctctgcgcgg ttcacttcct tgatgaggtc cccttcgcgc
     3001 aatccctcgg attgcgccag gcttccgggg tcgactttgg tgatcagtac gcctttgctt
     3061 tcacggagtt taaacttttc ggctaacccg gccgtcaaat cctgcacatc aagcccgagt
     3121 ctcacttccg tgcgcgcttg cggaagcgaa gtcaccacgg cggcatctcg tcgttccgtc
     3181 aatcccacct ccaaggtcag gcgcacctga tcacggacaa cttcgacctt ggctgtcgcc
     3241 cctggaagca agccagccac gagtcgcgag agacgattgg gagtatcgac aagattgcca
     3301 tcaatcttgg taatgatatc acccggcttg atcccggccg acgctgccgg atccttctca
     3361 aacacctcgt tgacgagcac cccttcccct tcgcggacac cgaacttacg tgccaactcc
     3421 ggcgtgagcg gttgaatgcc gacccccagc catccgcgaa ccacgcgccc ctttgccaac
     3481 agttgctgga tgacctgttt ggccatattg gccggactgg cgaacccgat gccctgtgca
     3541 aagttgatga tggcggtatt gatcccgatg acctcgccgt ggaggttgaa caatggccca
     3601 ccggagttcc ccggattgat cgaggcgtcg gtttgaataa aattctcata gcgcgacaga
     3661 ttgatatttt ctcgcccgat tccactgacc acgcccaagg tgaccgtccg gtccaggccg
     3721 aaagggttgc ctacggccaa aacccattgg ccaacacgaa ctttgcttga atccccgaac
     3781 ttggcatacg cgagcgggtg gtctggggtg accttgatga tcgccaaatc ggtatccgga
     3841 tccttcccaa taacctgtcc gatgagagtc gatttatcgg acaaccgaac ttctatttcc
     3901 gttgcatcgc cgatcacatg gttgtttgtg acaatgtggc cctcggcgtc aacaatcagg
     3961 ccggatccgg aacctgggga gttagggatt cgttcctgtg gcatttcgcg agcacgtcca
     4021 ggaccgctga gcggaaagag attgaccacg gacggctttg cagactccgc gagctcggtg
     4081 atgacagttt gaatttcctc cagcatttga aggccagccc ccgagcgcgc tggagttgtg
     4141 tctggctcag cgcccgccat ggccactgga tcaagcaggg tcaacagggt gacagacaga
     4201 aggggagcga tgtaacgtgc catcctcata gcaagtgctt gtattctata ccacgctcgt
     4261 tctctatgcc cactttagat acgttaggcg gaccgaacgc ggtcagcaac cgtcagctgt
     4321 gcgaccgcgt gagcaagatc agtttgcagt cccaccacga gcagaacatc ctcactccga
     4381 agcaccagat cctcagacga attgatgaaa aatggtccgc ggaagatcgt cactacacga
     4441 accgattccg gaaggcgcag ctcaccgatc tgtcgtccga tcgccggcgc cccgggtgac
     4501 aagacaatgc gctcaagggc ggcagatcgc cggctgaggt ctcgctggag agtaagcaaa
     4561 tcttcctggg tcgtctcaag tttcagcgct cgcacctgac tatcgactgt caggagcatc
     4621 tgcttgagtt ggtggacgcg ggcagttgtt tggaccacca gttggttcaa ttcggtggca
     4681 acggaggctt cgtgatcagg cagatatcta cgggtccaca gagcggtgat ctgacggccg
     4741 agttctgcat gcgcgatatc aacctgggtg atgaccgcgg aagcctggcc atggaggcga
     4801 aggacttcga ctttccggtt cacccgctcg gcaacggcta tgatcgtttc atagatggcc
     4861 tgtcctgtga tggagagctc tttttgaagg cggccgagga actcaagaaa cataggagct
     4921 ccgtgataca aggtcagata agatatatta tgtaaacttc tacttctctc ccgttcccgt
     4981 gctccgatcc tttactctcc tcccccttct tcggtcaaca tattcccgcg acggtgtcct
     5041 cccttctcat cctctccatc cttccaagtc gcagactgga tataacactc tccatacgac
     5101 aatagcttcc ttcgttggat caaaagaatt aaaaccacaa ttgccggccg ttcatcgtaa
     5161 ttattgccag atcctgattc gcagtgggtg caactcgtgg cgaggtcttg cctgtgactc
     5221 ttgtccagtc gagaaccgga atgtcacatg atttgatgat gggagactct ccgatggact
     5281 agaacatcgc cgattctcca tttcgaaaga gtggtcgtat tatcgtgatc gctcatcaat
     5341 ggtccgcttt gtaatgcccc tatgatcttc ccgtgctgaa ccttcacact atccccgttc
     5401 acaaacgcca tcgcccgtcc ccatctatag gacaacgact tcgctctcgt tggaatcttg
     5461 atcgcgaccc atcgattctc ggaccaaaac accacgtacc ccccctcttg gtgtgattga
     5521 acggcgcagc cgtcttctct cacaaacgct cgccctcccg gaagccattg gaacgtacct
     5581 tgaagcagac agtcggatcc gtctgcaaat gccaggacca gccatgcaag cgtctcgacc
     5641 cccgtcatcc tccacacctc tgtgaagcgg attacgtgct aagagtcccc cagccccagg
     5701 ggcatgtctg tttgaagcct agcactcagc cgcaggatgt aaaggaatgg gtgaggaacc
     5761 gcgaccttaa tgagggcaag gagaaaccct tgctgggctg gaggcaactt gccgttcggc
     5821 atggtaggaa ctgcggacca atggtcctga ttcgacgtgg ataaaaccga gagcgtgccc
     5881 ttcctcctcc agggctacga attcgtcagg atgataaaaa cgagcgactg gcaggtggtc
     5941 tttcgtcggc tggagatatt gcccgatggt catgatatcg cagtcgatgg aacgcagatt
     6001 gcgcatgacc tcgcgggctt catccatcgt ttcgcccatg ccaaggatga gtccggactt
     6061 tgtggtcatc cccaactcct tcgctcgccc taatagcgca attgaacgtt gatactttcc
     6121 ctgaggtctg acggtcggaa acagccgctg gacggtttcg atattatgat tcaggatatc
     6181 tggtttctcg gcgcatacgg tcgcgagcgc cgcattattc ccttcgaaat ccggaatgag
     6241 tacttcgatc gtgcaagtcg gaatcagctt tcggatgtat ctgatggttt cggcaaagac
     6301 ggaggccccg ccatcttcca actcatcccg gtttaccgag gtgatgaccg catggcgcag
     6361 gccgagggct tggactgcat gggcgactcg taaaggctcc tcgcggtcta attccattgg
     6421 tcgaccggtg gtgaccgagc agtagtggca ccgcctcgta cagatgtcgc ccagaatcag
     6481 aaaggtcgcg gtgcgggcgt tccaacattc ccacacgttg ggacaacggg ct
//
LOCUS       DATDNW010000172         7048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_565634, whole genome shotgun sequence.
ACCESSION   DATDNW010000172 DATDNW010000000
VERSION     DATDNW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565634"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VI359_04220"
     CDS             <1..1432
                     /locus_tag="VI359_04220"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY6973491.1"
                     /translation="VSDGISMGTEGMKASLISREVIADSIELVARGHLFDGVVALSGC
                     DKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHDVTIQDVFEAVGKHASGKMTTAEL
                     KDLEDHACPGPGACGGQFTANTMAIAFEFLGISPMGRNGVPAMDQKKDDVAFECGKMV
                     MDLLKHDLRPRQIITRKSLENAIAAVATTGGSTNAVLHLLAVAREMGVKLTIDDFDQI
                     NRKVPLLADLKPGGRFTAADLYAAGGTTLVAKRLLAAGILHAGQPTVTGRTIGEEANE
                     AKETAGQQVLRPLSNPIKPTGGLVILKGNLAPEGCVVKVAGHSMMTFRGPAKVFDREE
                     DAFVAVQARQIKTGDVVVIRYEGPSGGPGMREMLGVTAAIVGAGLGDSVALLTDGRFS
                     GATHGLMAGHVAPEAIKGGPIAAVKNGDMIRFDIAKRRLDVELSQKEIAARLKKVKRP
                     VSRYTSGVMAKYARHVSSASEGAITT"
     gene            complement(1429..2211)
                     /locus_tag="VI359_04225"
     CDS             complement(1429..2211)
                     /locus_tag="VI359_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973492.1"
                     /translation="MKFIPTEIVHNLLMGLQPVAKFAQRYHSTGINADPEMVNRVFDL
                     YTRFVSIQGKDILEIGPGQTLEILERARAEGARSCAAIDVVDYRLPEQAKKPSITCVV
                     YGGRKVPFESDRFDLIWSYTAFEHLRYPELTVKECFRVLRPGGCLVALIDLGDHSYYG
                     CAAIGPEKLFDCLRYPEWLWNLMRWNRSSYVNRLRRSDWIRLFDEAGFIVRTQEAMVS
                     QDVARLLPQLKYLQKYSHNDAVTSVLTVCLEKPLKASASQPN"
     gene            complement(2308..3153)
                     /locus_tag="VI359_04230"
     CDS             complement(2308..3153)
                     /locus_tag="VI359_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973493.1"
                     /translation="MSAKDSSDRYLPAASVALFVLFLWTTGVLAKSDLDRFELRGPVN
                     TVVTKYPQLRTTHQFDRNGLLTSLELFQADGRDSARYVFVHDATGRVIEEQTFEPDGT
                     VAYKKIFRYGFDEGGRQTALVAATDTGAFTNAEFAIYDHRGFLTEELSISGQGLIEKS
                     LFDVRGNLVYHARYYQGKLVQEATHHHTALGRLKESRYYGFDGELMRKDLYRYNEAGQ
                     LIEQQSEVFHQSHLRKTVVTFEFDHAGNWIKETVQQWTDKGGSLALAETVVSRERLLT
                     YYGPE"
     gene            complement(3209..4207)
                     /locus_tag="VI359_04235"
     CDS             complement(3209..4207)
                     /locus_tag="VI359_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973494.1"
                     /translation="MEQATILVVEDETIVAKDIQCSLQRLGYHVPSTATSGEEAIRKA
                     GELKPDLVLMDIVLKGRMDGIETAQQLQHQYDVPVVYLTAYADHQTLDRAKTTTPAGY
                     MLKPFQPTELRTTIELALHRSRSNRHVKESLRWLMTTVRCIQEAVVTTNRDGQVTYLS
                     PAAETLTGWTLQELVELDLRVLLQAGKEMSSVTVENPARRAIAERNVVQLDGLYLKKK
                     NGAIDEISGRAAPVSDDGGTIMGAVVVFQERKGTELVRGEPGGSDHRFARPAGIINLC
                     AWCKRVPDESGHWYDLATFISEHSLLQFNGGLCPECMTKCFPREVQGPEHSCVLEP"
     gene            complement(4211..6067)
                     /locus_tag="VI359_04240"
     CDS             complement(4211..6067)
                     /locus_tag="VI359_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6973495.1"
                     /translation="MSIAEKYVGREIRWCPMTLLQAHYPVVAAILALIGGIFAVDWWL
                     PQTLAITVLYVVPVLLASRIHLPWLTSRVAILTSVLTLMEMIRTPLLNLTWVVVCNRS
                     FTLLVIWVTAVLCLHRQRDEARLRKVCQAIEQQVEERTADLAHANKLLDALHAEAVSQ
                     LAAIVKSSDDAIVGMTLNGIIRSWNAGAEQVYGYTADEMVGRSISLLCPPDRLDEVPV
                     MLGRIARSEHVRHVETVQRRKRGTPIDVSLTVSPVKDADGIVIGASSIARDITARKRT
                     EAALRESEARFHMMADTAPVMVWMAGPDGRCTFVNKRWLEFTGRSLDDEVGDDWITGL
                     HPTDAERCQKTYTGSFRTEQPFSMEYRLQRSDGKYRWVLDTGVPFFEPGGRFGGYIGT
                     CVDMTERKDMEDRLRKALKDKESLLREVHHRVKNNLQIISSLLNLQISSIKDPQVAQL
                     FRECQVRITSIALLHDTLHRSDNLSRIAMSEYMRTLISHLFRSYGVDGTRIQANLQLD
                     EVEFDIDTGLTCGLILDELVSNCLKHAFNDHRPGCITISLQAHADDTYSLRVSDNGVG
                     LPKDGVLNNPDSLGLELVGLMAEKLDGTITLQSGQGTEWQVRFRPLQYQERM"
     gene            complement(6464..>7048)
                     /locus_tag="VI359_04245"
     CDS             complement(6464..>7048)
                     /locus_tag="VI359_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY6973496.1"
                     /translation="RIGDHVVAIGHPEQGGLWTLTTGVISAEVDNFNGVKGKNVFQTE
                     TGLNRGNSGGPLLDGEGRMVGINTAIARVASDGLPITSISFSLKSSVATQWLKDQHVA
                     IQQANGTTAGMGQTRVATDEPMSVPPVQSPPQAKPAPAPKAEGLNKPPVKPDIADSGP
                     ARPYDLDKLISERSKVEADLDNMISEMRGRMKGR"
BASE COUNT         1487 a         2043 c         1965 g         1553 t
ORIGIN      
        1 cgtgtccgac gggatctcga tgggcaccga ggggatgaag gcgtcattga tcagccgaga
       61 agtgattgcc gattcgatcg agctggtggc gcgcggtcac ctctttgacg gcgtcgtggc
      121 actgtcaggt tgtgacaaga caatccccgg caccgtgatg gccttggcac gcttgaacgt
      181 gccgtcattg atgctctatg gcggttcgat catgccgggt cagttccaag gccatgacgt
      241 gacgattcaa gatgtcttcg aagccgtggg caaacatgcc tcgggcaaga tgacgactgc
      301 cgagctgaag gacctcgaag accatgcttg tcccggcccc ggtgcctgcg gaggtcagtt
      361 cacggccaat acaatggcga ttgcttttga atttctcgga atttccccga tgggacgcaa
      421 cggtgtgccg gcgatggatc aaaagaaaga cgacgtggcg ttcgagtgcg ggaagatggt
      481 gatggacctc ctgaaacacg atctgcgacc ccggcagatc attacccgaa agtcgctgga
      541 gaacgcgatc gcggctgtcg ccacaaccgg aggctcgacg aatgccgtgc tgcacttgct
      601 cgccgtggcc cgagaaatgg gtgtaaagct cacgatcgac gatttcgacc agattaatcg
      661 taaggtaccg ttgctcgccg acctcaaacc ggggggccgg tttacagcgg cggacctcta
      721 tgctgccgga ggaaccacgc tggtggccaa gcggctactc gcggcgggaa ttcttcacgc
      781 cggtcaaccc acggtgacgg gccggacaat cggggaagaa gccaacgagg cgaaggaaac
      841 agccggccag caagtcctca gaccgttgtc gaatcccatc aagccgaccg gcggattggt
      901 catcctcaag ggcaacctgg ctccggaagg ttgcgtcgtc aaagtggccg gccactccat
      961 gatgaccttc cgcggtcccg ccaaggtttt tgatcgggag gaggatgcct ttgtcgccgt
     1021 ccaagcacga cagatcaaaa ccggagatgt cgtggtgatc cgttatgaag gcccctcggg
     1081 cgggcccggc atgcgtgaga tgctgggagt gacggcggcg atcgtcggcg cgggactggg
     1141 agactcggta gcactcctta ccgacggccg cttttccggc gccacccacg gactgatggc
     1201 tggccatgtc gcaccggaag ccatcaaagg cgggccgatt gccgccgtga agaacggtga
     1261 catgatccgg ttcgacatcg cgaaacggcg gttggacgtc gaactgtctc aaaaagaaat
     1321 cgctgctcgg ctgaaaaagg tgaaacggcc ggtttcccgg tatacctccg gcgtgatggc
     1381 caagtacgca cggcacgtct cctcggcgtc cgaaggagca attaccactt agtttggttg
     1441 agaggcggac gctttgagcg gtttttccag acagaccgtc aggacacttg tgacggcatc
     1501 gttatgacta tacttctgca gatacttcag ctgcggaagc aagcgggcga cgtcttgact
     1561 gaccatcgcc tcttgtgtcc gcacgataaa tcccgcctca tcgaatagtc tgatccagtc
     1621 cgatcgcctc agccggttca catatgagct acgattccat ctcatgagat tccatagcca
     1681 ttctggataa cgaaggcagt cgaacaactt ctcggggccg attgcagcgc acccatagta
     1741 cgaatgatct ccaaggtcaa tgagcgcgac gaggcatcct cctggccgga ggacccgaaa
     1801 acattccttg acggtgagct cgggatagcg caaatgctcg aaggccgtat aggaccaaat
     1861 gagatcaaag cggtctgatt caaacgggac ctttctcccc ccatatacta cgcatgtgat
     1921 ggacggcttc ttcgcctgct caggcaatcg gtaatccacc acgtcgatcg ccgcacagct
     1981 ccgtgctccc tccgctctcg cccgttccaa gatttccaat gtctgaccag ggccgatttc
     2041 taagatgtct ttcccctgta tggacacgaa tcgtgtatac aggtcgaaca cccggttaac
     2101 catctcaggg tcggcattga ttcccgtcga atgatatcgc tgggcaaact tcgccaccgg
     2161 ttggaggccc atgaggagat tgtgaacgat ttccgttgga atgaacttca acaggcaagt
     2221 tcctctggga actgatccct ggctcggtct tttatccctg gatccatacc ggaagaatct
     2281 cccggacacg catccgggca catcgcttta ttctggtccg tagtaggtca agagccgctc
     2341 ccggctgacg acggtttcgg ccaaggctag gctcccaccc ttgtccgtcc actgctgaac
     2401 ggtctctttg atccagttgc cggcatggtc gaattcgaag gtcacgaccg tctttctgag
     2461 gtgagactgg tgaaagactt cgctctgctg ttcgatgagc tgacccgcct cgttgtaccg
     2521 gtagaggtcc tttctcatca actctccatc gaacccgtaa tatcggcttt ccttcagacg
     2581 accgagggcg gtgtggtgat gcgtcgcctc ttgcaccaat ttcccttggt agtaacgagc
     2641 gtggtagacc agattacctc gaacatcgaa gaggcttttt tcgatcagcc cttggccgga
     2701 gatggaaagc tcttcggtca ggaatccgcg gtggtcatag atcgcaaact ccgcattggt
     2761 gaaggctccc gtgtctgtgg cagccacgag cgcggtttgc cgccccccct cgtcgaagcc
     2821 gtatcgaaag atcttcttat aggcaaccgt gccatcaggc tcaaaggtct gttcctcgat
     2881 cacgcgccct gtcgcatcat ggacaaacac gtaacgggct gagtctctcc catcggcttg
     2941 gaagagttcc aagctcgtca gaaggccgtt tcgatcgaat tgatgagtgg tccgtagctg
     3001 gggatacttc gtgacaaccg tattcacagg cccgcgcaac tcgaatcgat ccagatcact
     3061 ttttgcgagt acgcccgtcg tccagaggaa caagacaaac agagcgacgc tcgcggcagg
     3121 aagataccga tcggaggaat ccttcgccga catgccaagg cggtatcata cacggcctgc
     3181 aggatttgag aaaccccttc tcaccacgtc agggttcgag aacacacgag tgctcaggac
     3241 cttgcacctc gcgcgggaaa cacttggtca tacattccgg acagagtcct ccattgaatt
     3301 ggagcaagga gtgttcactg ataaaggtcg cgagatcgta ccaatgtcct gactcatccg
     3361 gcacccgctt gcaccaggcg cagaggttaa tgatcccggc cggcctggca aagcggtggt
     3421 ccgatcctcc cggctctccc cgcaccagtt cggtcccttt cctctcttga aagacgacta
     3481 cggcacccat aatcgttcct ccgtcgtcgg atacaggcgc cgcgcggcca ctgatttcgt
     3541 cgatggctcc gttcttcttt ttcagataca gtccatccag ttggacaacg ttccgttcag
     3601 cgatcgcccg ccgagccgga ttctcgaccg ttacggatga catctctttg ccggcctgta
     3661 acaggactct gagatcaagc tctaccagtt cttgcagcgt ccagccagtc agcgtttccg
     3721 cggccgggct taaataggtc acctggccgt cccggttggt ggtcacaacg gcctcttgga
     3781 tgcagcgcac tgtcgtcatc agccagcgca aactctcctt cacatgtcga ttggaccgcg
     3841 accggtgcag ggccaattca atggtcgtgc gcaactcggt gggctggaac ggtttgagca
     3901 tgtatcctgc cggcgtcgtc gttttagccc gatccaaggt ctgatgatca gcataggcgg
     3961 tgaggtacac gaccggaaca tcgtactgat gctgcagctg ctgtgcggtc tcgatcccgt
     4021 ccattcgtcc tttgagcacg atgtccatga gaaccaaatc cggcttgagt tcgcccgctt
     4081 tacggatcgc ctcctcgcct gacgtggccg tactgggtac gtggtaccca agccgctgga
     4141 ggctacactg aatatctttc gccacaatag tttcgtcttc tacaacgaga atggtggcct
     4201 gttccatgat tcacatcctt tcttggtact gcagcggacg gaaccggacc tgccattcgg
     4261 tcccttgccc gctttgtaac gtgatggtcc catccagttt ttccgccatt agaccaacca
     4321 gctccagacc cagagaatcc ggattgttca agactccgtc cttgggcagg ccaacaccgt
     4381 tgtcgctgac tcggagggag taggtatcgt ctgcatgcgc ctgcagcgaa atcgtgatgc
     4441 atcctggccg gtggtcattg aatgcatgtt tgagacaatt ggatacgagt tcatcgagaa
     4501 tcagaccgca cgtgagcccc gtatcgatgt cgaactcgac ctcatcaagc tgaaggttgg
     4561 cttgaattct ggtgccgtcc actccgtagg accggaagag atggctgatc agcgtccgca
     4621 tatattcgct catggcgatc cgtgacaggt tgtcggaccg gtgaagcgtg tcatggagca
     4681 aggcgatcga cgtgatgcgc acctggcatt cacgaaacag ctgagcaacc tgcggatctt
     4741 taattgagct gatctgaagg ttcagcaggc tcgagatgat ctgcaagttg tttttcaccc
     4801 ggtgatggac ctcacgaaga agactctctt tgtccttcag agccttgcgc aatcgatctt
     4861 ccatgtcctt gcgttcggtc atgtctacgc atgttccgat atagcctcca aatcgaccgc
     4921 cgggttcgaa aaacggcact ccggtatcca aaacccatcg atacttccca tcgctccgct
     4981 gtagccgata ttccatcgaa aacggttgct ccgtcctgaa cgaacccgtg taggttttct
     5041 ggcaccgttc ggcatcagtc gggtgaagac cagtaatcca gtcatcgccg acctcatcgt
     5101 cgagcgagcg cccggtgaac tcgagccacc gcttgttcac gaaggtacag cggccatccg
     5161 gaccggccat ccacaccatt accggagcgg tgtccgccat catatggaat cgagcttcgc
     5221 tttcgcgcag agcggcttca gtgcgcttcc tcgccgtaat gtcccgggcg atcgacgagg
     5281 cgccaatgac gatgccgtcc gcgtctttta ccggagaaac ggtcaatgac acatcgatcg
     5341 gcgttcctcg cttccgtcgc tggaccgttt cgacgtggcg gacatgctcg ctgcgtgcga
     5401 ttcggcccag catcaccggt acctcgtcga gtcgatcagg aggacacagc aatgagatcg
     5461 atcgcccgac catttcatcg gccgtatagc catagacttg ctccgccccg gcgttccaac
     5521 ttctgatgat gccattgaga gtcataccga caatcgcgtc gtccgaggac ttcacaatcg
     5581 ccgcgagctg cgagacagcc tccgcgtgta gtgcgtccaa cagtttgttg gcatgtgcca
     5641 gatccgccgt tcgctcttcc acctgctgtt cgatggcctg acagacttta cgcagccgcg
     5701 cttcgtcgcg ctggcggtgg agacagagca ccgcggtcac ccaaatgacc agaagagtga
     5761 atgatcgatt gcacactacc acccaggtga ggttgagcag cggggtgcga atcatttcca
     5821 tcagggtcaa gacggacgta aggatcgcca cacgcgacgt gagccagggt aaatgaatcc
     5881 gcgacgccag cagaactggc accacataca gcacggtaat cgcaagagtc tggggtagcc
     5941 accagtcgac agcgaagatc cctccgatca aggccaggat cgcggcgacg acgggataat
     6001 gggcttgcag aagcgtcata gggcaccagc ggatttctct acctacatat ttctcagcaa
     6061 tgctcatgcc gctcgctcta atcgatcctt aatccacgag gccctggtga gagtgcactc
     6121 ttgcacaaat tcagctgtcc gatttcttct ctgcccttct ttgagaacca catgggctat
     6181 gtttgctagt cccttcgcct catcactggt tggattccac caaggcccac gacaaagttg
     6241 tacagagcgg tgtggaaatc gcggaggccc gcaaacggac tgagggaaac ccctaggttt
     6301 tgcgcgtacg atttaggaag caggaatgtt cagccttgaa cgggttgaag gcaggcggaa
     6361 gcgcagagtg gggatgaaat ccgatacatg gagaaagcaa cttgagcgag aagaaggcga
     6421 cgatcggccg gaaagtgaga cgatgaaatc gcgtgttcga cggttaccgc cctttcatcc
     6481 taccgcgcat ctcactaatc atgttgtcga gatcagcctc gaccttgctc cgctcgctga
     6541 ttaacttatc gaggtcatag ggtcgagcag gaccggaatc ggcgatgtcg ggtttgactg
     6601 gcggtttatt caaaccttct gcttttggtg cgggagctgg cttggcctgt ggaggggatt
     6661 gaacgggcgg gacggacatg ggctcgtctg tcgccactct ggtttgcccc atcccggctg
     6721 tggtcccgtt cgcttgttgg atggcaacat gttgatcctt caaccactgg gttgccacac
     6781 tggatttgag cgagaagctg atgctcgtga tcggaagtcc gtcagaggcc acgcgggcga
     6841 ttgctgtatt gatcccgacc atgcgtcctt ctccatccag cagcgggccg cctgaattgc
     6901 cgcgattcaa tcccgtctct gtctgaaata cattcttgcc cttcaccccg ttgaagttgt
     6961 caacctctgc gctgatgacg ccagtggtca aggtccagag tcctccttgc tcgggatgcc
     7021 cgatggccac cacatggtcc ccgatcct
//
LOCUS       DATDNW010000173         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_289901, whole genome shotgun sequence.
ACCESSION   DATDNW010000173 DATDNW010000000
VERSION     DATDNW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289901"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..490
                     /locus_tag="VI359_04250"
     CDS             68..490
                     /locus_tag="VI359_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973497.1"
                     /translation="MRLHSSSVAAVSKMRHKISADESAARRTKTDTPERRREVRTVRM
                     RLCTFGMMHATERGVVTLEEGEGTVVDDSGTGMRLLLGIAPLEKQLLEIQTVQSFLKR
                     SIYLVEVCWTKPVREDAQGRLFLVGGRLLFGPSHYWLS"
     gene            642..1412
                     /locus_tag="VI359_04255"
     CDS             642..1412
                     /locus_tag="VI359_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6973498.1"
                     /translation="MTTRTAPQKQRISLDRSIERMRKQLAELAPFLGKGKATRSLEEF
                     DLETERLIGDLLGQTSDLLHAYEYAELGEAAGLVNMTDEAPEGTGMDSHRQSLLQRSR
                     VLESCVAELEARRAAEPKRAKANRQTLIGPQVAEHMSPEIRSLSQDASLREAGQLMQK
                     WKLGSLLLTDNQSYVGFITDSALAREVVANGVNPNSTAVKTCMRKPVVSIAGDRPIID
                     AVRMMKEQATRHLAVTQDGQIIGVISVSNILRYYSGVV"
     gene            1443..1802
                     /locus_tag="VI359_04260"
     CDS             1443..1802
                     /locus_tag="VI359_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973499.1"
                     /translation="MGPTKAIVKGNALHEAVGDKLIKDGFANRREIEEYVNHHYLVLP
                     VVDNAGKPWVLEGKPVYCLRGSQYETVDDQKVHLARCPDCGGMGIRSDEFTVESDCIR
                     CTVCGHEFDARLEMMET"
BASE COUNT          417 a          577 c          619 g          391 t
ORIGIN      
        1 ttcgtgcgat tgaaagaggc gctgcgtccg tgacgtctgg gggagtggtt cacgtcgcaa
       61 gcgactcatg cggttgcact cgtcctccgt ggcggcggtt tcgaagatgc ggcataagat
      121 tagtgctgat gagagtgcag ctcgccgtac caagacagac acccctgaac gtcggcgcga
      181 agtccgaacg gtccggatgc gtctctgcac atttggcatg atgcacgcga cagagcgcgg
      241 cgtggtcacg ctggaagagg gagaaggcac cgtcgtcgat gacagcggga caggcatgcg
      301 ccttctgctg ggcatcgccc ctctcgagaa gcaactcctg gaaattcaga ccgtccaatc
      361 gttcttaaag cgctcgattt atctcgtcga ggtctgctgg accaaaccgg tgcgggaaga
      421 tgcgcagggt cgattattcc tcgttggcgg ccggcttctg tttggtccct cccactattg
      481 gctttcttag ctcgggccgg aaaccgtcaa tctccgcccc tgtccttcag ggatcacgtc
      541 ctatcaagca cccagtacag cacctaggct agtcacttgc ctcgccgatc cagcgggagg
      601 tatgatcggc ccacccctga gtcatctaca gaggaggtgc tatgaccacg cgcaccgctc
      661 cccagaaaca acggatctcc ctggaccgca gcatcgagcg catgcggaag cagcttgccg
      721 aactcgcccc gttcttgggc aagggcaagg cgacccgcag cttggaagaa ttcgacctgg
      781 agacggaacg gttgatcggc gatctcctgg ggcagacctc cgacttattg catgcgtacg
      841 aatatgcgga gttgggcgag gcggccggat tggtgaacat gacggatgag gcgccggaag
      901 gcaccggcat ggatagccat cgtcaaagcc tcttgcaacg gagccgagtg ttggaaagct
      961 gtgtggccga attagaagcg cgccgggctg cggaacccaa gagagccaag gccaatcgcc
     1021 agactctcat cggcccgcaa gtagccgaac acatgtcccc cgaaatccgg agcctatccc
     1081 aggacgccag cctgcgggaa gcggggcagc tcatgcagaa atggaaactc gggtcgctcc
     1141 tcctcacgga caaccagtcg tatgtcggat tcatcacgga ttcggccttg gcgcgcgagg
     1201 tcgtcgccaa cggggtgaat cccaacagca ctgccgtcaa aacgtgcatg cgcaagccgg
     1261 tcgtctccat tgcaggcgat cgcccgatta tcgacgccgt gcgcatgatg aaagagcagg
     1321 ccacccggca tctggccgtg acccaggacg gtcagatcat cggcgtcatc tcagtctcga
     1381 atattctgcg gtactactct ggcgtagtct gatcagcctc aatccgactg gggaggtggg
     1441 acatgggccc aacgaaggcg attgtgaagg gaaacgcgct gcatgaagcc gtgggagaca
     1501 agctgatcaa ggacggcttc gccaaccggc gcgagattga ggagtacgtg aaccatcatt
     1561 atctcgtgtt gccggtggtc gacaacgcag gcaagccctg ggtcctcgag gggaagccgg
     1621 tctattgcct gcgtggaagt caatatgaaa ccgtggatga tcagaaggtc caccttgcgc
     1681 gttgtccgga ttgcggtggc atgggaatca gatcagacga atttacggtc gaatcggatt
     1741 gcatccgctg cacggtctgc gggcatgagt ttgatgcgcg gctggaaatg atggagacgt
     1801 gaggggacgg cctggttggt tccctgtgct cacggaaccc ccacgatgaa gctctggtca
     1861 ttcaacgtgc gcagtgggga gcccaccaac gctgtcgccc cggcatcttt ctctcaactt
     1921 tctcgtctct ttgccccgcc gcggttgcct ggccacacac ctaacaaggg ggtcggtatg
     1981 gatgtgtcgc tcctattcgc actg
//
LOCUS       DATDNW010000174         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_841666, whole genome shotgun sequence.
ACCESSION   DATDNW010000174 DATDNW010000000
VERSION     DATDNW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841666"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VI359_04265"
     CDS             complement(<1..1364)
                     /locus_tag="VI359_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973500.1"
                     /translation="MGLKPATNPEVEAAAIELSIDGKTVTAKDGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKATAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDLPLSCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQMYIETKDTEVIRITSPLSWKNKWGDRADTSKGH
                     GGLCVRGRFGFEYLDSPSRLKQPMVRKGNQLVETPWLETMHHVVERFSEIRKRHGPEA
                     IAGLITARCPNEELYLFQKLMRAGFRTNNLDSSARYGHLNFVHASRQALGIGRSPNDW
                     EDLTKAKAVLLIGSNITETNPLTAVRIKEAIRVYKAQVVTIDSAITNMGKLASHPFVI
                     KAGT"
     gene            complement(1389..>2085)
                     /locus_tag="VI359_04270"
     CDS             complement(1389..>2085)
                     /locus_tag="VI359_04270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY6973501.1"
                     /translation="ERDFTPSLLEKLKKFLDYFPPKIDEYVVFLEKNRIWLARTKGVA
                     VISAEDAVSFGLSGPTLRGSGVDYDLRKYEGYAAYPKCEFSVPVGKNGDTYDRYWIRV
                     MELYESVKIIRQCLEQMQDGPIMADVPSVTLPPKERVFTNLESMIQQFKLFSQGFNAP
                     PGEIYCGTEAHKGELGFYIVSAGGGKPYRLKIRAPSFIHMGAFDYMSRGYMIADAVTI
                     FGTYDIVMGECDR"
BASE COUNT          424 a          580 c          578 g          503 t
ORIGIN      
        1 tcggttcccg ccttgatcac gaatggatgt gaggcaagct tgcccatgtt cgtgatcgcg
       61 gaatcaatcg tgacaacctg ggccttgtag acacggatgg cttccttgat ccgcacggcc
      121 gtgagcggat tggtttctgt aatgttcgac cctatcagca agacggcctt cgccttggtc
      181 aaatcttccc agtcgttcgg tgagcgtccg attcccagcg cttgacggga ggcatggacg
      241 aaattcaagt ggccgtaacg cgcgctgctg tcaaggttgt ttgtgcggaa tccagctcgc
      301 atgagtttct ggaagaggta caactcttcg ttcgggcagc gtgcagtaat caagccggcg
      361 attgcctccg gcccgtgacg cttgcggatc tcggaaaatc gctcgacgac gtggtgcatc
      421 gtttcgagcc acggagtttc gacgagctga ttccccttgc ggaccatcgg ttgtttcaag
      481 cgcgaggggc tatcgaggta ttcgaatcca aatcgtcctc ggacgcagag cccaccatgg
      541 cctttcgagg tatcggcgcg atcaccccat ttatttttcc acgacagcgg cgacgtgatg
      601 cgaatgactt cggtgtcttt ggtttcaatg tacatctggc agccgtcccc gcagtagttg
      661 caggtggtgg tcgttttctt catctgccag ggcttgtaga ggtactttga aaacttgttc
      721 gtgatcgcgc ctaccgggca gaccgccaga caatctccgc agaactcgca ggacagtggc
      781 agatcgccct tcgccacgac ttggttaaat ccgcccttct tcatgaactg taaggcgtca
      841 atcatcagca catctttaca gacgttgatg cattcagcgc aggcaatgca gcgattcatg
      901 ttgaagtcga ggacgaggct tctggtatct tcgggaatga atttctgctt cgcgttggcg
      961 aggttcgtca cgccgtgttg aaacgccatg tcttggagtt cgcagtgacc gtcggcgtcg
     1021 cagaccgggc aatctagcgg gtggacggag agatgcttct cgacggcttt cttcctggcc
     1081 tggaaaaggt cgtcgccctc cgtgcggata atcattccgg cagtcgcctt ggccgtacaa
     1141 gaccggaccg gggccttctt cccctcctgc atgacgaggc acattccgca ggaaccgaaa
     1201 ggatcaaagg tgtagtggta gcacatggcc ggaataatct tgcccgtgct cgaaatgaca
     1261 tcgtacagcg agacgccgtc cttcgccgtg acggttttcc cgtcaatcga gagttcgatc
     1321 gccgccgctt cgacttcagg attggttgct ggtttcaaac ccatagtgcc taccttgctc
     1381 tagtcattct atcgatcaca ttcgcccatc acgatgtcgt acgtgccgaa aatcgtgacc
     1441 gcatccgcga tcatgtagcc cctcgacatg taatcaaacg cgcccatatg gatgaaggag
     1501 ggggctcgaa ttttgagccg gtagggtttt cctccacccg cgctcacgat atagaacccc
     1561 aattcgccct tatgggcttc agtcccgcaa taaatttctc cagggggagc attaaagccc
     1621 tgcgagaaga gcttgaattg ctggatcatc gattccagat tggtgaagac ccgttccttg
     1681 ggaggcagcg tgacgctggg cacatccgcc atgattgggc cgtcttgcat ctgctcgaga
     1741 cattgccgga tgatcttgac gctttcatag agttccatga cgcggatcca gtatcgatcg
     1801 tacgtatcac cgttcttgcc gaccgggaca ctgaactcgc acttgggata ggccgcgtag
     1861 ccttcgtact tgcggaggtc atagtccacg ccggagccgc gcaaagtcgg accgctcaag
     1921 ccgaaactca ccgcgtcttc cgcagaaatc accgcgacgc ctttggtccg ggcgagccaa
     1981 atccggttct tctcgaggaa gaccacgtat tcgtcgattt tcggcgggaa gtagtcgaga
     2041 aacttcttga gcttctccaa cagtgatggt gtgaaatccc gttcg
//
LOCUS       DATDNW010000175         4235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1112949, whole genome shotgun sequence.
ACCESSION   DATDNW010000175 DATDNW010000000
VERSION     DATDNW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112949"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VI359_04275"
     CDS             complement(<1..203)
                     /locus_tag="VI359_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY6973502.1"
                     /translation="MGYRAFCIRVATQRRLSGGVKNLEDGRVELEVEGRRDLILALIE
                     ELKIGPPAARVGTVDVEWAAATGR"
     gene            complement(743..819)
                     /locus_tag="VI359_04280"
     tRNA            complement(743..819)
                     /locus_tag="VI359_04280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Pro,seq:cgg)
     gene            complement(859..1962)
                     /gene="ald"
                     /locus_tag="VI359_04285"
     CDS             complement(859..1962)
                     /gene="ald"
                     /locus_tag="VI359_04285"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY6973503.1"
                     /translation="MIIGVPKEVKDHEYRVSVTPDGVQALTQGGHQVWVEPSAGVGSG
                     YSDDDYRRVGAAIAGSKGELFRKAELIVKVKEPLLSECSFFRPGQVLFTYLHLASLPG
                     LTKALLEAKVTAIAYETTEGRDGGLPMLKPMSEIAGRMSIQIGARYLEKTQGGRGVLL
                     AGVPGVEPAHVVVLGAGIVGSAATRIAVGMGARATVINLDLDRLRALDDLYQGRIVTR
                     ASTPGAIEQSVRDADLVIGAVLIPGARAPKLVSRALVKQMKPGAVVVDVAVDQGGCFE
                     TTKPTTHSDPVYLVDGVLHYGVANIPGIVPRTSTVALTNATFPFLSRLASAGVQQAIR
                     SDAGLAKGVNLMEGKVTCRAVAEAHGLRFDPLL"
     gene            2278..2508
                     /locus_tag="VI359_04290"
     CDS             2278..2508
                     /locus_tag="VI359_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973504.1"
                     /translation="MQKNLQRDFRDIRAEYDKLSAGQGSALSNTDEFRRASLRLREIV
                     TILDAATSPSPLIATADVKLLLERLSTATMPV"
     gene            2521..2874
                     /locus_tag="VI359_04295"
     CDS             2521..2874
                     /locus_tag="VI359_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY6973505.1"
                     /translation="MPPFQRHIFVCINQRPKDDPRGCCANLGSEKLHAHFKSETKRLN
                     LKGLVRANKAGCLDHCSMGPSVVIYPEGVWYWVGNEADVTEIMERHVLKGEIVTRLLM
                     PDHPVPAKLAPLKMD"
     gene            2928..3311
                     /locus_tag="VI359_04300"
     CDS             2928..3311
                     /locus_tag="VI359_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973506.1"
                     /translation="MEKVAFMKRFPGLLHSWDSLAGKTVEAVIELKSKTGSAVVIFTD
                     GSFTVLPHPSTEPYELGEALTVARQYLEPKHREAYAEHDRLVKKDKDAQKSARLSNIL
                     GAIQNNVDQIPELKDKLKALVKEWK"
     gene            3377..3607
                     /locus_tag="VI359_04305"
     CDS             3377..3607
                     /locus_tag="VI359_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973507.1"
                     /translation="MMIIRDHLVRHPDRCTHQAICVPICPTGAWLSTPPFKFDASRCL
                     ESCRLCLDACPSQAIFGVFKKGEKLLEPQPKK"
     gene            complement(3966..>4235)
                     /locus_tag="VI359_04310"
     CDS             complement(3966..>4235)
                     /locus_tag="VI359_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973508.1"
                     /translation="KSVVAAVLDNALPIGDYQYNMTLPTGAPALAAFGPLPITPTAAA
                     QSAVAKMYTVQGSAQTATTVYTTQTPAPSSKDITANPMQDFTLTP"
BASE COUNT          935 a         1262 c         1143 g          895 t
ORIGIN      
        1 cgccctgtcg ccgcggccca ttccacatca accgtgccca cacgagccgc cggaggaccg
       61 atcttgagtt cctcaatgag tgcgagaata agatcgcgcc tcccctccac ttcgagttct
      121 actcggccgt cttctagatt cttcactcct ccggagagcc gtcgttgagt cgcgacccgg
      181 atgcaaaacg ctcgataccc cacaccttag cactcgtcct gctacgatga ttcgtgcccg
      241 aaccggggcc tgtgcagtcg actcagtcat gagctcagct gcctaagagt ccaccagccc
      301 gctccaccaa cggatactct catacctcat tagaaccgtc acgcgttttg caaattggtg
      361 ccgcgtagca gcccagatgt cacgagactg ccgaatgaaa gcgagagaag acactactga
      421 gggtgaaaca tttttccagt cgcctgaata gaagaggagg ctccgggtcg agcaagctct
      481 gggcacggcc gtggacgagg aatcacgtta atggggtgaa gcctctttcc cgtcgcctca
      541 agtggaggaa gaggctccgg gtcgagcaga atctggaata ggataacact gatggagttt
      601 ttccgcgggc gatcgatacg atacaaccat gaagagcccg cggtacgagc tagctaatca
      661 atgaggaagc atctgaatgg agcgaggctt ctttcccgtc gcttcaatag aagaggaggc
      721 tccgggtcga gcaagctctg tttggtcggg gcgagaggat ttgaacctcc gacccctgcg
      781 tcccgaacgc agtgcgctac cgggctgcgc tacgccccga cttgatcacc aggaaaacga
      841 aacgagggtg ggattgtctt acaacagagg gtcgaaacgc aagccatgtg cttcggccac
      901 cgcgcgacac gtgactttcc cttccatgag attcacacct ttggcgagac cggcatcgga
      961 gcgaatggct tgttggacac cagcggatgc caaacgtgag aggaacggaa aggtggcatt
     1021 tgtcagtgcg acggtggatg tccgcggcac gattccaggg atattcgcca caccatagtg
     1081 caacacgcca tcgaccagat agaccgggtc cgagtgtgtc gtcggcttcg tcgtttcgaa
     1141 acatcctcct tgatcgacgg ccacatccac aacaactgct ccgggcttca tttgcttgac
     1201 caaggcgcgc gacacgagct tgggagccct tgccccaggt atcaacaccg ccccgatcac
     1261 cagatccgcg tcgcgaacgg attgttcaat cgcaccgggg gtagaggcac gtgtgacaat
     1321 ccgaccttga tacaaatcat ccagggcgcg aagccggtcg agatcgagat tgatgacggt
     1381 cgctcgagca cccatcccca cggcaattct cgtcgccgca ctgccgacga tccccgctcc
     1441 caatacgacc acgtgggccg gttcgacacc cggcactcct gccaagagga cgccccgtcc
     1501 tccttgagtc ttttccaaat accgcgcgcc aatctgtatc gacatccggc cggcgatctc
     1561 gctcatcggc ttcaacatcg ggagtccccc gtctcgccct tccgtcgtct cataggcgat
     1621 cgcggtgacc ttggcctcca gcagagcctt cgttagacca ggcagcgatg ccaggtggag
     1681 ataggtaaac aggacttgtc caggccgaaa gaaggagcac tcggacagca gcggctcctt
     1741 cactttcaca atgagttcgg ccttgcgaaa aagctcccct ttcgatcccg cgatcgcggc
     1801 gccgacccgt ctataatcgt cgtctgaata cccgctgccg acaccagctg agggctctac
     1861 ccagacttgg tgccctccct gcgtcagcgc ctgcactccg tcgggtgtca cactcacacg
     1921 atattcgtga tctttgactt cttttggaac accgataatc actgtctgcc tcctgccagg
     1981 cgactgcgta tctctacttg cattatatac ggtattaacg aacctgtcct tacctcccgc
     2041 gttccccgct gtggacagtg tgtaaagccg tggtgtaaga ttcgctcgtc tcacacacaa
     2101 ttgtctgagc ctggaggacg ggccatggac gcaacggctg gttcctgtaa cgcctgcggt
     2161 gccacaggca ctgcgctgat aaagttttct ctcggccgag atttttttgg gcgatcgtat
     2221 gatcgcctct ccccttcagc cgatcaaagc cccaaatggt attgcgagac ctgttcgatg
     2281 cagaagaatc tgcaacgaga ctttcgcgat atccgcgcgg aatacgacaa attgagcgcc
     2341 ggtcaaggct ctgctctctc gaataccgac gagttccgac gtgcctcgct gcgattgcgt
     2401 gaaatcgtta ccatcctcga cgctgccacg agcccatcgc cgctgattgc aacagcagac
     2461 gtcaagcttc ttctggagcg gctcagcacc gcaaccatgc ccgtctaacc ctattgcttt
     2521 atgcctccgt tccagcgtca tattttcgtc tgtatcaatc aacggccgaa agacgatccg
     2581 cgcggctgct gcgcgaatct tggttcagaa aagctgcacg cccattttaa gagcgagacc
     2641 aagcgtctca atctcaaagg gttggtgcgc gcaaataagg ccgggtgcct cgaccattgt
     2701 tcgatggggc ccagcgtggt catctatccc gaaggtgtct ggtactgggt tggcaatgaa
     2761 gctgatgtca ccgagatcat ggagcgacac gtcctgaaag gcgaaatcgt gactcggttg
     2821 ctgatgccgg accatccggt gcctgcgaag ctcgcaccgc tgaagatgga ctagctcctt
     2881 cgccgattac attcaccatg ccaattgaag acaaatggaa ggccaacatg gaaaaggtgg
     2941 ccttcatgaa gcggtttccc ggcctgctgc actcgtggga ctccctggcc gggaaaacgg
     3001 tagaagccgt gattgaattg aagagcaaga caggatcagc cgtcgtcatt tttaccgacg
     3061 ggtcttttac cgtcctccct catccttcca cagaacccta cgagctcggt gaggccttaa
     3121 cggtcgcacg ccagtatctt gagccaaagc atcgtgaggc ctatgccgag catgatcgtc
     3181 tcgtgaagaa ggacaaagat gcgcagaaat cagctcgact cagcaacatt ctgggcgcca
     3241 ttcagaacaa cgtggaccaa attccggaat taaaagacaa gctgaaagcg ctcgtgaagg
     3301 agtggaagtg agcagcctcc ccgagaaaaa catgtttgag atcttttccc aaggtctctt
     3361 tgaaggagtc aaacccatga tgatcattcg cgatcacctt gtccggcatc ccgatcgttg
     3421 tacgcatcaa gctatctgcg tccccatctg cccgaccggc gcctggctct cgacgccgcc
     3481 gttcaagttc gacgcctccc gttgtctcga aagttgccgc ctctgtttgg acgcctgccc
     3541 gtcgcaggcg atctttgggg tgttcaagaa gggggagaag ctgctggagc cgcagccgaa
     3601 gaaatagccg gcagggctga cgaacggcgc acgacctcgg ttgcgccatc atgcgatgac
     3661 tactcacaac cgttgatctg cgtcttaatt cgccgcgggc tcctctaaaa cgctgcctct
     3721 ccggcagcat gccgtcgccc gctcacctac atttagccac tgaccctcac ctcacgcgat
     3781 gtcgccaacc tcgccgtgca tgtgttctag cccagcaggt gagaaacaag atggaaacag
     3841 accgcttcta ttcgtcaagg gcggtctcat cgccacagat ccgttttgta tttggatgtg
     3901 acagtctgtg agacgagaga gctttgccgg ccgcaggtga tcacccacgg ccggcagccg
     3961 atggtttacg gagtcaaagt gaagtcctgc attggattgg cagtaatatc ctttgacgaa
     4021 ggcgcggggg tctgagtcgt ataaacagtc gtggcagtct gggcggaacc ctggaccgtg
     4081 tacatttttg ccaccgcgga ttgcgcagcg gcagtcggtg taataggcag cgggccaaac
     4141 gctgcgagcg caggcgctcc agtaggcagc gtcatattat attgatagtc accgattggt
     4201 agagcattgt ccaaaactgc ggcgactacc gattt
//
LOCUS       DATDNW010000176         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_20185,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000176 DATDNW010000000
VERSION     DATDNW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20185"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..175
                     /locus_tag="VI359_04315"
     CDS             23..175
                     /locus_tag="VI359_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY6973509.1"
                     /translation="MKDRLCDDTGELRRFVNIYVNEEDIRFLKGKDTSLKDGDEVSIV
                     PAIAGG"
     gene            178..417
                     /locus_tag="VI359_04320"
     CDS             178..417
                     /locus_tag="VI359_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEY6973510.1"
                     /translation="MSHLRFHIRFPEDKVKEPIIYQIGREYNIVTNVRRADVRETTGW
                     ADVELSGETAEIERAIDGLRKKGCVVDPIELNVVE"
     gene            511..1314
                     /gene="moeB"
                     /locus_tag="VI359_04325"
     CDS             511..1314
                     /gene="moeB"
                     /locus_tag="VI359_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY6973511.1"
                     /translation="MEFTEQQIQRYSRHIILQDVGGKGQLKLAKAKVLLIGAGGLGSP
                     AGLYLAAAGIGTIGLVDGDVVDLSNLQRQILHSTATVGQPKVESGKKTLAAINPDITI
                     KAYHMNVDADNILGLISDYDIVLDGSDNFTTRFLVNDACFFAKKTLISASMFRFEGQL
                     TTIKPQAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TLADRLLIYDALEMKFRKVGRPKDPACRLCGPKPTITDLSSDYTVTCTI"
     gene            1321..1833
                     /locus_tag="VI359_04330"
     CDS             1321..1833
                     /locus_tag="VI359_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY6973512.1"
                     /translation="MPDLFIPQQIIDGMIAHARELDPYECCGILAGRNGTVTQIYRIK
                     NIVALDGAEKLSSFDPAKVAHLERLSPAERAEIAFVMDMQDFSAAKKDIRNKDLDLQV
                     VYHSHPHDPARPSVTDIKIASDYEEIWPKINLPIPAYLLISLMDKNKPDIRTYWIKDG
                     TVSPASVVIV"
BASE COUNT          495 a          607 c          527 g          442 t
ORIGIN      
        1 gctcaacagc gcccatccgg gcctgaagga tcgcctctgc gatgataccg gcgagcttcg
       61 gcgcttcgtc aacatctacg tcaacgaaga agatatccgt ttcctcaaag ggaaagacac
      121 ttcactcaaa gacggcgacg aagtgtcaat cgtccccgca atcgctggag gctgacgatg
      181 tcacatctac gttttcacat tcgctttccg gaggacaagg tcaaggagcc gatcatctat
      241 cagatcggga gagaatataa catcgtgacc aatgtccggc gggctgatgt gcgtgaaacg
      301 accggctggg ccgatgtgga actctcgggc gagacagctg aaattgaacg ggccatcgac
      361 ggccttagaa aaaaaggctg tgtcgtagac ccgatcgaac tgaatgtggt cgaatgacag
      421 gcacgcggcg acaggcgcga ggcgagtggc aaatcttcac tttctccacc tctagccgct
      481 ggccccgcgc cactaaccta tagcaacgct atggaattca ccgaacaaca aatccagcgt
      541 tacagccgcc acatcattct ccaggatgtg ggtggtaagg gtcagctgaa gctcgccaag
      601 gcgaaggtgt tgttgatcgg tgcgggcggg ctcggctcac cagccggcct ctacttggcc
      661 gctgcgggga ttggcacgat cggtctcgta gacggcgatg tggtggatct ctccaatctc
      721 cagcggcaaa tactccactc gaccgccacc gtgggtcagc ccaaggtcga atcgggaaag
      781 aaaacgcttg ctgcaatcaa tccggatatc acgatcaagg cgtatcacat gaacgtggat
      841 gccgacaata ttctcggatt gatctccgac tatgacatcg tgctggacgg atccgacaac
      901 ttcacgacgc gttttctcgt gaacgatgcc tgcttctttg ccaagaaaac gctgatttct
      961 gccagcatgt tccggtttga ggggcagttg accacgatca aaccgcaggc aggataccct
     1021 tgctaccgct gtctgtatcc ggaaccgcca ccggccggac tggtgccgaa ttgccaggaa
     1081 gccggcgtgc tcggcgtcct cgccggaacc atgggcatct tgcaagcgtc cgaagccatc
     1141 aaggagattc tcggtatcgg cgaaacgctt gcagatcgcc tcctgatcta cgatgccctc
     1201 gagatgaaat tcaggaaagt cggccgcccg aaagatcctg cctgccgtct ctgcggtccc
     1261 aaaccgacca ttaccgattt aagcagtgat tacaccgtca cctgcaccat ctaaccaacc
     1321 gtgccagacc tgttcattcc acaacagatt attgacggca tgattgccca cgcccgggag
     1381 ttggatcctt acgaatgttg cgggattctg gccggcagga acggcacggt cacgcagata
     1441 tatcgcatca agaatatcgt ggcgctggac ggagccgaaa aattgtcgtc gtttgatccc
     1501 gccaaggtgg cgcacctcga acggctctct cccgcggaac gagccgaaat cgccttcgtc
     1561 atggacatgc aggacttctc cgccgcgaag aaggacatcc ggaacaaaga cctagatctg
     1621 caagtcgtct accactctca tcctcacgat cccgcgcgac cgtcggtcac cgatatcaag
     1681 atcgcatccg attatgagga aatctggccc aagatcaatt taccgatccc tgcatatctc
     1741 ctcatttcat tgatggacaa gaacaagcca gacatccgaa cctattggat caaggacgga
     1801 acggtctctc cagcctctgt tgtgatcgtc taaatacagt tcgcctcacg tcccacacgc
     1861 caattttcct gttcccttct ttttatccga atgccataat ggcacatcat ttaccttcat
     1921 ccgaggagtg cccgccatgc agactacgcg cacattcctg ataggagcac tggttctctg
     1981 gttcctcttc ctcgtcatca ttcctcccac gatggctgaa gaacggagtt tctatagtcc
     2041 cgtgatcttc atcgataagg agaagaacca a
//
LOCUS       DATDNW010000177         8855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_633663, whole genome shotgun sequence.
ACCESSION   DATDNW010000177 DATDNW010000000
VERSION     DATDNW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633663"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VI359_04335"
     CDS             complement(<1..771)
                     /locus_tag="VI359_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY6973513.1"
                     /translation="MKLTGAEIFIECLKREGVKTIFALPGGVVLKIFDMLYQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVALVTSGPGMTNVITALADAYMDSVPLVCFSGQV
                     PTHLIGNDAFQEADNVGLSRPCTKYNFLVKDVTELATTIKEAFYIATTGRPGPVLVDI
                     PKDVAQNQTEFHYPNSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGGGVIFSG
                     ASQELRELAEMTQIPVDMTLMGLGAFPGDHPLSMGMMGM"
     gene            complement(1034..1461)
                     /gene="rnpB"
                     /locus_tag="VI359_04340"
     ncRNA           complement(1034..1461)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VI359_04340"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1538..1909)
                     /locus_tag="VI359_04345"
     CDS             complement(1538..1909)
                     /locus_tag="VI359_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973514.1"
                     /translation="MASRSGARVPLECAVVYVGELAIGKGRICNVSLSGCQLEGAHKT
                     MKRGDSVHLRVFIPGHQIPLSVPLAVVRWVEKNRVGLEFIRSSREDQTRLARFVRQQK
                     QLAGIWYKWNESKTKLATLVD"
     gene            complement(2058..2927)
                     /locus_tag="VI359_04350"
     CDS             complement(2058..2927)
                     /locus_tag="VI359_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6973515.1"
                     /translation="MIRVRWVTRIMLLILSSSSLFVLNGCETNPYTGRSQLLMTSVSE
                     EMQLGAQAYQQVKNDPKVHQSQDPREIEPVKRVAARIIEAAKRSKYAEMAKQFQWEVT
                     VIKDDKTMNAFALPGGKIAVYTGIFPVAKTEGGLAAVLGHEVTHALARHGAERMSQGE
                     LTNAALQVVGAAAGASGMNPTLSQGAMAALGAGAQVGVLLPFSRSHESEADYIGILLA
                     ADAGYDPRESVHLWERMEQLSGGGGPSEFLSTHPGHETRIAQLKKWMPEAMGIYQTRQ
                     PVPAAELPPISAR"
     gene            3041..3709
                     /gene="rnhC"
                     /locus_tag="VI359_04355"
     CDS             3041..3709
                     /gene="rnhC"
                     /locus_tag="VI359_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEY6973516.1"
                     /translation="MTATTPHPSIERIGIDESGKGDYFGPLVIAAVFVDATTQGELSL
                     MEVRDSKKISDGRILEMAPDIRTICPHSIIAIGPQRYNELYDKIRNLNRLLAWGHAKA
                     LETLLERVSCDRAIADQFGDERLILNALQEKGRKITLEQRPKAEIDLAVAAASILARA
                     EFLLRLKRLSTEVGTTLPKGASPAVELAARMIVKKHGQDRLSTVAKLHFKTTKAVLGA
                     VAPT"
     gene            complement(3706..4482)
                     /locus_tag="VI359_04360"
     CDS             complement(3706..4482)
                     /locus_tag="VI359_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY6973517.1"
                     /translation="MNSNLSPLIELQKFDLRIMEIKDERRHIPQRLQAVEAPLQEAKR
                     VLHDTTSAVDALVKERRSYEKDLEAHEDRVEKLKERASQLKTNQEYQAHLFEVDLANK
                     KKFEIEDKILGCMEKIEQLQRVSKEAQEKVTVAEKSFAQEKQMLEERDQKLMQELAEL
                     EVQQRERAAHIDKNLLDRYTKLKAARKDHALASVQGGMCSGCRLQIPPQLIAEVKRSQ
                     DLHSCPYCHRMLYWDGDGSTDQKPPQDQVKPSDLEVGESV"
     gene            complement(4542..4618)
                     /locus_tag="VI359_04365"
     tRNA            complement(4542..4618)
                     /locus_tag="VI359_04365"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4582..4584),aa:Ile,seq:cat)
     gene            complement(4720..6528)
                     /gene="rpoD"
                     /locus_tag="VI359_04370"
     CDS             complement(4720..6528)
                     /gene="rpoD"
                     /locus_tag="VI359_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY6973518.1"
                     /translation="MPKQELLVEVKKLIAIGKEKGFLTYDELNSTLPAEVVSSEQFGS
                     IMAMFGEMDIEIVDAPEGGGERPRKSRDSEDASDDDEADAGEDNEKEIDLTPGALSRT
                     DDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKRDIASVIYGMPMTIEFVLSLREQLK
                     NGKIDVREIVPIQETEEDFEEEEVEKDYEELRLKTLEGLNSVRKISTSLKGLYEKSRH
                     VNGDPTKLKKIKKQIDTIREQIVDKMESVNLHGVLKDRMVQRVRDLAIQIRTAEREIV
                     SCQRRLGIGGEAGAEALRKLCRDRKSFLTVKRKSGCSEDTLSDVKKLFQAAKTRIRQL
                     ETEEALVSADEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFLDL
                     IQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINK
                     LIRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSHLG
                     DFIEDKKAISPLEAAIRYDLQRQINSALETLTPREEKVLRKRFGIGEATDHTLEEVGQ
                     DFEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESM"
     gene            complement(6531..8348)
                     /gene="dnaG"
                     /locus_tag="VI359_04375"
     CDS             complement(6531..8348)
                     /gene="dnaG"
                     /locus_tag="VI359_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY6973519.1"
                     /translation="MGRGLISEDVINQIRDRIDIAEVIGQHVNLTRAGQNLKGLCPFH
                     QENTPSFTVSSSRQIFHCFGCGAGGNVFGFLMRITGASFPDTVRELGEKVGIEVKESA
                     GGTGSQGTQSGRIEQLNRAVASWFQDNLRDQTIGAPARDYLASRGIEVPIIERFGIGY
                     VPPDWDGLSTALAKQGFSASEQAAAGLTVAREHSSGFYDRFRSRVMFPIIDLRKRVVG
                     FGGRILGEGTPKYLNSPDTPLFKKGQTLFALDAAREAIARLKTVIVVEGYFDAIALHQ
                     AGLTHTVATLGTALTPEHIQVLRRFASKVVLLFDPDQAGVRAALRGLDLFVNSGMGVT
                     VVTLPDGEDPDTYVRKEGPESFARLEAQAPSLLDFALEQSLKVAQSSTLEERIRSVDE
                     VLRILQKSEHPIEREERIRLVAERLGINQQRLIERYPALIQKPAPRPIARRSETAPSA
                     QLFKGTPEERDLVFLLLQGQLSPADVRRLRPETFSVPSCRIIVETALACLDRDGRVGL
                     RNLLDAVVDHPECGAVATELSMREDHFDDVRAHVKGCLDTLDRKRAEAVLRELIAQLK
                     EAEREGRVEDARSLNARVNELRMQKAGQSSAGSLSLVKE"
     gene            complement(8628..>8855)
                     /locus_tag="VI359_04380"
     CDS             complement(8628..>8855)
                     /locus_tag="VI359_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY6973520.1"
                     /translation="LVIPKRHVVSVQELGEQDQALLGRLVLACTKIAKLKGLAENGYR
                     LVTNTGRDGGQSVFHLHFHILGGRHMAWPPG"
BASE COUNT         1857 a         2710 c         2226 g         2062 t
ORIGIN      
        1 catccccatc atgcccatgg acaacggatg gtcgccgggg aacgcaccca atcccataag
       61 tgtcatgtcg accggaatct gtgtcatttc ggcaagctca cgcaattcct gtgaggcacc
      121 ggaaaatatc acgccgccac ccacgtacag gatgggcttc ttggccttca tgatggcttc
      181 ggcggcttgc ttgatttgcc acttattccc ttcgtacgtc gggttatagc cgcgaatcga
      241 gacggagttc ggatagtgaa actccgtttg gttctgcgcg acgtccttcg gaatatcgac
      301 cagcactggt cctgggcgcc ccgtcgtcgc gatatagaag gcttccttaa tcgtggtcgc
      361 tagctcggtc acatccttga ctagaaagtt gtacttggta caggggcggc tcagtccgac
      421 attgtctgct tcctggaacg catcgttgcc gatgagatgc gtcggcacct gaccgctgaa
      481 acacaccaac ggcaccgaat ccatgtaggc atcggccagc gccgtgatca cattcgtcat
      541 cccaggacca gaggtgacca gggctacacc ggccttgccg gtcgcctttg catacccttc
      601 cgccatgtga ccagctccct gttcatggcg ggtgagtacg acttcaatat ccttttgctg
      661 atagagcatg tcgaaaatct tcaagacaac gccacccggc agcgcgaaga tggttttcac
      721 cccttcccgt ttcaggcatt cgatgaagat ttcagccccc gtgagcttca taccaaccct
      781 cctctccctc gccattcctg gatttctcag cagagggaac cactcgacat gcgtggaaga
      841 cgatacaaac accttcccca cgatttttca gaagcatctg aaaaatcaag gggaaagatg
      901 cgcgatccta tcatcacccc ttgcacccgt caataggatg ccgggttacg gcgatttcca
      961 tcagcctcgt cgccgctcac gagaatacgg tggataaaga ataagaagga cgggcagtga
     1021 ttatataaaa gcgcgctgga gagaacctat aagccggatt ctgtcccacc tgggctttga
     1081 atcccagacg ggggtgatca tttctctggg attcgagttg cctcgaacct caagcgacct
     1141 acccgaagac ctcggccggg ccagccgttc cgcacgatcg cgaacgatca cgcgggtgtc
     1201 ttcctatttg gccttgcatc gggcaacgct taccgtgccg gtgatgtcac caccaccgcg
     1261 gtgggctctt accccaccgt ttcaccctta cctgatccgc agcgactaga aaggggtccc
     1321 atcgccttgg ccatcggcgg tctattctct gtggtgccgg tgtcggatcg ctccgcctgg
     1381 gtgttaccca gcgccccgcc cctgaagtcc ggactttcct ctcgcagcgt tgccgccacg
     1441 agcgatcacc cgatcttctc caacgcgcag gatcagtata ggaaaggttt gaagaggttg
     1501 caagggcaag tcgagagcag aacggccaac aaggggctta atctaccaat gttgcgagct
     1561 tcgtttttga ttcattccac ttgtaccaga tccctgccag ttgcttctgt tgcctcacaa
     1621 aacgtgccag ccgggtttgg tcctccctag aagaacgaat aaattccagt cccactcggt
     1681 ttttttcaac ccagcgcacg acggccaggg gaacactcag cggaatctgg tgacctggta
     1741 tgaatacacg gaggtgcact gaatccccgc gcttcatcgt cttgtgggcc ccttcgagtt
     1801 ggcacccaga gagggagacg ttgcagatcc ggcctttgcc gatggccagc tcccccacat
     1861 agacgacagc acactcaagc gggacacgag ctccagatcg agaggccatt ggtacctcct
     1921 aagtgagaag agtcattttg aacagaatcc catacctcac tacccccgcc aatcctacga
     1981 aaggcgggac ttgcgcagta cgaaaggagg tgcttagaga tctcagctcc ggccgtaaag
     2041 gcctcgaatg cttacactta gcgggcagat atgggaggta gttcagctgc tggaacaggc
     2101 tgacgggttt gataaattcc cattgcctcc ggcatccatt tcttcagttg cgcgattctg
     2161 gtttcatggc ctggatgagt cgacaagaac tccgacggtc cgcctccgcc agaaagctgt
     2221 tccattcgtt cccagagatg gaccgactcc cgtggatcat agcctgcatc cgctgcgagt
     2281 aatatgccga tgtaatctgc ttcagattcg tgcgatcgac tgaatgggag caacacgcca
     2341 acctgcgcgc ccgcccccaa tgccgccatc gcaccctgag acaatgtggg attcatgcca
     2401 ctggcgcctg cggccgcccc aaccacttga agcgccgcgt tcgtgagttc accttggctc
     2461 atccgctcgg cgccgtgacg tgccaaggcg tgagtcactt catgccctaa gactgcagcg
     2521 agcccccctt cggttttcgc aacaggaaag atgccggtat acacggcgat tttcccgccg
     2581 ggtagcgcga acgcgttcat cgttttgtcg tccttgatga cggtgacttc ccactgaaat
     2641 tgcttcgcca tctctgcata ttttgaacgt ttggccgcct cgatgatacg ggccgcaact
     2701 ctcttgaccg gctcgatctc acgaggatct tgcgactggt gcactttagg atcatttttc
     2761 acctgttgat aggcctgcgc cccaagctgc atctcttccg agacagaagt catgagcaat
     2821 tgagaccgtc cggtataggg attggtttcg cagccgttca gaacaaacag cgagctactg
     2881 ctcaggatca acagcataat gcgtgtgacc cacctgaccc taatcatggt tgatcctccc
     2941 cgctgaatcc ctgtccatta gtgtggccaa attgaccgat cttcaatgct ctgttagatt
     3001 accgcgccgc accacgattt tctacaaggg atactcgcct gtgaccgcta ccactccgca
     3061 cccatccatc gaacgcatcg gcatcgatga atccggcaag ggagattact tcggtccact
     3121 ggtgatcgcc gctgtctttg tcgatgcgac aacgcaaggc gaactttccc tcatggaggt
     3181 acgagacagc aagaaaattt ccgacggccg cattctcgaa atggccccgg acattcgcac
     3241 gatctgcccc cacagcatca tcgcgattgg tccccagcga tacaatgagc tctacgacaa
     3301 gattcgcaat ctgaatcgtc tgctcgcatg gggtcacgcc aaagcgctgg aaactctgct
     3361 ggagcgcgtc tcttgcgacc gagccatcgc cgatcaattc ggcgatgagc gattgattct
     3421 gaacgcgttg caggagaaag gtcgaaagat aactctggag cagcgaccca aagcagagat
     3481 tgatctcgcc gttgcagccg cctcgattct cgcgcgcgcg gaattcttac tgagactgaa
     3541 acggctctcg accgaggtcg gcaccaccct ccccaaaggg gcgtcgccag ccgttgaact
     3601 tgccgcccgc atgattgtga agaagcacgg gcaagaccgc ctgagtaccg tcgccaagct
     3661 ccatttcaag accacaaagg ccgtgcttgg agcagtggct cctacctaga cggattcgcc
     3721 gacttcgaga tcggaaggtt tgacctgatc ctgcggtggc ttctgatcgg tcgacccatc
     3781 gccatcccaa tacaacatcc gatggcaata ggggcaggag tgtaaatcct gggaacgttt
     3841 cacttcggca atcagttgcg gaggaatttg aagccgacac cctgaacaca tgccgccttg
     3901 caccgaggcc agtgcatgat cttttctggc tgctttcagc tttgtgtacc ggtcgagcaa
     3961 gttcttatca atatgagctg cccgttcccg ttgttgcacc tcgagttcgg ccagctcttg
     4021 catcagcttc tgatcgcgtt cctccagcat ctgcttttcc tgtgcgaagg acttctccgc
     4081 aaccgtaacc ttctcctgcg cttccttcga aacccgctgt agttgttcga tcttttccat
     4141 gcaacccaag atcttgtctt cgatctcaaa tttcttcttg ttcgccaagt ccacttcgaa
     4201 cagatgggct tggtattcct ggttggtttt taattgcgaa gcccgctcct tcaatttttc
     4261 aacgcgatct tcgtgagctt caagatcctt ttcgtaactc ctccgctctt tcaccagcgc
     4321 gtcaacggcg gacgtcgtat cgtgcaacac ccgctttgcc tcctgtagcg gagcctcgac
     4381 tgcttgaagc cgttgtggga tgtgtcgacg ttcgtctttg atctccatga tacggagatc
     4441 aaacttttgt agctcgatga ggggagaaag attcgagttc aatgcactcc tacatatctg
     4501 aggtgcaaca gcagcctgac tctgctgcga accggcctcg ctggtgggcc cactaggact
     4561 cgaacctagg accagctgat tatgagtcag ccgctctaac cacctgagct atgggcccca
     4621 tcagggagtc tgcggttgac ccatcgcgcc tgacccgcag acacgagcgg ttgattgtag
     4681 gacgccgccg aaatggagtc aattgacgcc agcacccggc tacatacttt cgacaaagct
     4741 gcgcaacttc ttgctgcggc tcggatggcg taacttgcgc aatgccttgg cttcgatctg
     4801 acggatacgt tcgcgcgtca cctcaaagtc ttgaccgacc tcctcgagtg tgtgatccgt
     4861 cgcctcgccg atgccgaacc gtttccgaag caccttttct tcccttggcg tcaacgtttc
     4921 cagcgcgcta ttgatctgcc gctgcaagtc ataacgaatc gccgcttcca agggtgagat
     4981 cgccttcttg tcttcgatga aatcgcccag atggctgtct tcctcttcac caatcggagt
     5041 ttcgagtgaa atcggctccc gcgcaatctt caggatcttc cgaaccttat caagcggcaa
     5101 gtccatccgc tctgcgattt cctccggggt cggttcacgt ccgagctttt gcacgagatg
     5161 ccgggatgtc ctgatcagtt tattgatcgt ctcgatcatg tggacaggaa tacggatcgt
     5221 cctggcttgg tcggctatcg ctcgcgtaat cgcctgcctg atccaccacg tggcataggt
     5281 gctgaacttg tatccgcgct tgtattcgaa cttatccacg gccttcatca agccgatgtt
     5341 tccctcttga atcaaatcca agaattgaag cccgcggttc gtgtactttt tggcgatact
     5401 gacgacgagc cgcagatttg cctcaaccaa ttcggccttc ccgcgcttga ccttttcttc
     5461 cgccacgtcg agatgtttga cggcatcctt gatctcatcg gcagacacca gtgcttcttc
     5521 ggtttccaat tgcctgatac gggtcttggc cgcctgaaag agttttttga cgtcggagag
     5581 ggtatcttct gaacaccccg acttgcgctt gacggtgaga aaactcttgc gatcccggca
     5641 cagcttcctg agggcctccg cgcctgcctc accgccgatc ccgagccgac gctgacaact
     5701 gacgatctcc cgttctgccg tccgaatctg gatcgccaag tcccgcacgc gctgcaccat
     5761 acggtcctta agcacgccgt gaaggttgac ggactccatc ttgtcgacga tctgttcacg
     5821 gatcgtgtcg atctgctttt tgattttctt caactttgtc ggatcgccgt tgacatggcg
     5881 agacttctca tacagaccct tgagtgaggt cgagattttt cgaacggagt tcaatccttc
     5941 gagtgtcttc agtcgaagtt cctcataatc tttctcgact tcctcttctt caaagtcttc
     6001 ctcggtctct tggatcggca caatctcgcg gacgtcgatc tttccgttct tgagctgctc
     6061 acgcaatgac agcacaaact cgatcgtcat cggcatcccg tagataaccg atgcgatgtc
     6121 ccgcttgcct tcttcgatcc gcttggcaat ctcgatttct ccctcgcgac tcaggagcgc
     6181 gacgctcccc atctctttga ggtagagacg gacggggtcg tccgtgcggc tgagcgctcc
     6241 cggagtcaga tcgatctctt tctcgttatc ctcccccgca tctgcttcat cgtcatcgct
     6301 cgcgtcctcg ctatcgcgac ttttccgagg ccgttcccct ccaccttccg gagcatcgac
     6361 gatctcgatg tccatttcgc cgaacatcgc catgatgctc ccgaactgtt cggaggacac
     6421 gacttccgcg ggcaacgtac tattcagctc gtcataggtc agaaagccct tttctttccc
     6481 gatcgcgatc agcttcttga cttcgacgag caactcttgt ttcggcatga ctactccttt
     6541 accagagaga gagacccggc cgaggattga ccggcttttt gcattcgcaa ttcgttcacg
     6601 cgagcattga gcgatcgcgc gtcttccaca cggccctcac gttccgcttc cttcagctgc
     6661 gcgatcagct ctcgcagcac cgcctccgcc cgcttgcgat ccaaggtatc cagacatccc
     6721 ttcacatgag cccgcacatc gtcgaagtgg tcttcacgca tggagagttc cgtggcaaca
     6781 gctccgcact ccggatggtc gacgaccgca tcgagtaaat ttcgaagacc gacccgaccg
     6841 tctcgatcaa gacaggccag cgcggtttca acgattatgc gacaagacgg taccgagaac
     6901 gtctccggcc tgagtcgtcg cacatcagcg ggtgagagct gaccctggag caatagaaac
     6961 acgagatccc gttcttcagg tgtccctttg aacaactgcg cggaaggggc ggtctccgat
     7021 cgtcgggcta tcggccgagg cgcgggtttc tggatcagag ccggataccg ctcgatcaac
     7081 cgctgttgat tgattccgag tcgttccgct acgagccgga tgcgttcttc tcgttcgatc
     7141 gggtgttcgc tcttttgcaa aattcgcaag acttcatcca cgcttcggat acgttcctca
     7201 agcgtgcttg actgcgcgac ctttaggctc tgctccaatg cgaaatccaa aagacttgga
     7261 gcttgcgctt ccaatcgtgc gaacgattcc ggcccctcct ttcgcacata ggtgtcgggg
     7321 tcttcaccgt ccggaagtgt gacgaccgtc actcccatcc cgctgttgac gaacaaatcc
     7381 aacccccgca acgccgcacg gaccccggct tgatcggggt cgaacagcaa caccaccttc
     7441 gaagcgaatc gccgcaacac ctgaatatgc tcgggcgtca gggcggttcc cagcgtggcc
     7501 accgtatgtg tgaggccggc ttgatgcagc gcgatagcgt caaagtagcc ttcaacgacg
     7561 atcacggttt tcaaacgagc aatggcttcg cgggccgcat cgagcgcgaa gagggtctga
     7621 cccttcttga atagcggcgt atccggagag ttcaaatact tcggcgtgcc ttcaccgaga
     7681 atgcgccctc cgaatcccac cacacgctta cggaggtcaa tgatgggaaa catcacgcga
     7741 ctacgaaacc gatcataaaa cccggacgaa tgttcccgcg ccaccgtcaa cccagccgcc
     7801 gcctgctcgc tcgctgaaaa cccctgcttc gcgagcgctg tactcagccc atcccagtca
     7861 ggcggcacgt aaccaattcc aaaccgttcg atgataggta cctcaatgcc ccgactggcc
     7921 aaataatcgc gagcgggcgc cccgatggtt tggtcgcgaa ggttgtcctg aaaccacgaa
     7981 gcaactgctc gattgagctg ctcaattcga cccgactgcg tcccttgaga gcccgtaccg
     8041 cccgcactct ccttgacttc gattccaact ttttccccta gctcgcgcac cgtgtcagga
     8101 aaacttgccc ctgtgatgcg catcaagaat ccaaacacgt tgccgccggc gccgcagccg
     8161 aagcaatgaa agatttggcg cgaagaactg accgtgaagg agggggtgtt ttcctgatgg
     8221 aatggacaca accccttcaa attttgaccg gctctcgtca gattgacatg ctgcccaatg
     8281 acctcagcaa tgtcgatccg gtcccggatt tgattgatca cgtcttcaga aatcagtcct
     8341 cgccccacgg gagtcgcaac tcctcattcc ccctcgaaac agccaagggg ttttaaacgt
     8401 atcttctcga aaaaattgat cggtcagact atcagacagg caagaattct gtcaaacccg
     8461 cggaaatctc aagcggactg tcgacacgcc gatctacgca actcaaaaag gctgtccagc
     8521 tgggcctcaa agagtaagaa ctcccgggtg tatgcgagca gggcaggctg cgaggttcga
     8581 atgactcaga gcggcactgg tgctcttcgt caacatggta ttaggtatta gccaggaggc
     8641 caggccatgt ggcgaccacc caagatgtgg aaatgcaagt gaaagaccga ttgtcctccg
     8701 tctcggccag tgttcgtcac caaccggtag ccgttctcgg ccagcccctt gagtttagcg
     8761 attttcgtac aggccagcac cagccgtccc agcaacgcct gatcctgttc ccccaattcc
     8821 tgcaccgaca ccacatgtcg cttgggaatg accag
//
LOCUS       DATDNW010000178         8083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_980787, whole genome shotgun sequence.
ACCESSION   DATDNW010000178 DATDNW010000000
VERSION     DATDNW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980787"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VI359_04385"
     CDS             <1..775
                     /locus_tag="VI359_04385"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY6973521.1"
                     /translation="FVVSYRPPPQSMPTFFIAPEAITLPTIQIAGPLLHHLRESLRLH
                     RGESLILTDHQGIRYHVEVTDITSRAIHARVVDQETAPPRSTPQIVLGQALLKGEKMD
                     WVLQKATELGVDTIVPLETHHSVVKPNPERLEHQRNRWRRIVLEAAQQSERWTIPQVS
                     DPTTLARFVDRQSGATIKGILSERSKGSSLTSLMLPCEPHHMIVLAVGPEGGWTPEEQ
                     QLARDCGFVALTLGGRILRAETATLAALSILQSRLGELG"
     gene            complement(784..1623)
                     /locus_tag="VI359_04390"
     CDS             complement(784..1623)
                     /locus_tag="VI359_04390"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY6973522.1"
                     /translation="MNVYFRNPSLGWAVGSGGTVLKTLDGGQKWKKVSSGTTTLLTGV
                     LFVDEKHGWLVGANGTIRHSVNGGETWAPQPIDTQVPLYGISFVSPTEGWVVGGNGTI
                     LHTMDGGGHWVDQASTTTAALYAVQFVTDKRGTAVGAIGTVLATDDGGATWVAQETQG
                     AVTLFDVYFSDPSTGWVVGNAGAMFQTTDGGSRWVDRTLPCARNCTKLTDLLRVRFTD
                     PRTGWIVGERGMVYHTADSGFTWVERGSVAKVSLFGLSFPDALHGWATGEKGTIVRLS
                     TGQ"
     gene            complement(1784..3463)
                     /locus_tag="VI359_04395"
     CDS             complement(1784..3463)
                     /locus_tag="VI359_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY6973523.1"
                     /translation="MKALLTLLMSSAILWGSMTCAALAATSTTMQLTPSSATPGATVS
                     IGGKGFGSFRSVQFNRVTFSGVPALIQRWEPDLIEVKVPFQAQSGPVEVRIGKKTLAA
                     GTFSLVRPKITAVTPAAIEPGHPIEITGEHFGITAGARDPNTMFGVNDVVIGGVVVRP
                     RRWKDDKIEVEVPANAKSGDVLVRLASSDPLPDGSCCAPVRYAISNSVPISIIPTVRM
                     DPLQGPTGTKVVLFGEGFGASRDPSDGVRFGGHSAAVAQWSDNTIVVHVPSNAETGPV
                     VLKTRGQDRTVGTFTVQIPKATGISPASAPIGTLLRISGEHFGSYSESGSTPFNFMDF
                     DTGDNRVEIGGVPAVIYRWHDDRIDVWVPFSAKSGPVVVTRGSANPKPDGSCCEEKKL
                     VSTEAGTFTIVTPKIDSYSPHSAGLDEVVTIHGSGFGNFLKTTEASKMGMTKDIYKRN
                     SPTLEENVSRTEVLFSNVAAVVISWTDTEIKVRVPRRHLFGIGKPGEFNPDLSTGPLV
                     VRRGSWDILPDGTCCTPKKWLTIEAGTFTIEAKGFPDQSFFQVRPEGSTND"
     gene            complement(3658..4119)
                     /locus_tag="VI359_04400"
     CDS             complement(3658..4119)
                     /locus_tag="VI359_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY6973524.1"
                     /translation="MMSYEAKLKALGLQLPSPPQPVATYVPAVRAGDLLFLSGVLPMR
                     DGQLEFSGKLGRDLTVEEGMEAAKLCVLNALAIAKQELGSLDRINRIVKVVGHVASAE
                     GFVQQPQVVNGASDLLVAVFGESGRHARVAVGAAELPRRAPVEIEVILSIT"
     gene            complement(4131..5288)
                     /locus_tag="VI359_04405"
     CDS             complement(4131..5288)
                     /locus_tag="VI359_04405"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY6973525.1"
                     /translation="MMNRVILIVIDGFGIGAMPDAAKYGDESANTLVHLAEAAGGLHL
                     PTMEALGLGHITAIPSVQVMAQPEGCFGRLGFLSSDVDSLGGYWEMNGYMQAVAAHTY
                     PNGFPQPLIAVLEQISGRKMLGNRVASPTAMLQDYGNDHLASGAPIVWTDGRRTCHLA
                     AHATVMRPDALHQLCREARKLLREPWGIMRVVAHPLTGEKGAVRFSHQRRDFVIEPSG
                     TTMLDVLNRAGQILTGVGKAGDLFNGRGLTRTIPQAHWATLLEDVKGMLKNVPRGLIY
                     VGLDVLESDDAASAGALHDFDRRLPSLLEQLRPGDLFVVTGDHGRDPRKVHQGSTREY
                     VPLLMSGPKLAQGVNLGTRSTAADLGQTIVEALCGERLAVGDSFLDALRPG"
     gene            complement(5379..5702)
                     /locus_tag="VI359_04410"
     CDS             complement(5379..5702)
                     /locus_tag="VI359_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973526.1"
                     /translation="MFAIRKDIRFDVQCPVACVLEEQAVQGTTFNLSRGGCAIECGIM
                     ATEGETVSLEITVPGQPTPISVELGRICWATRREFGVEFKVVPGDSKQRLDAFLLEVA
                     KRSAS"
     gene            complement(5706..6386)
                     /gene="deoC"
                     /locus_tag="VI359_04415"
     CDS             complement(5706..6386)
                     /gene="deoC"
                     /locus_tag="VI359_04415"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY6973527.1"
                     /translation="MMTRDWNLPALIDHTILRPDATKQDVLRVCHEAKQAGFLVVFVP
                     PCYVDIAIAAVAESEIRVGSPIGFPLGWHTTRNKVAEALEAVGHGARILDMVINVSRL
                     RSGDHDYVRQDIAEVVRATPDAEHKVILETCYLTAQEKRTACQLAIEAGVEYVKTSTG
                     FAAAGATVEDVRLMKQAVAGKAKVKASGGIRDWKTTLAMLEAGADRIGTSASLTILAE
                     WSATRRKG"
     gene            complement(6453..7487)
                     /gene="mltG"
                     /locus_tag="VI359_04420"
     CDS             complement(6453..7487)
                     /gene="mltG"
                     /locus_tag="VI359_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY6973528.1"
                     /translation="MKLKVILGLLLVAVIVLGMAAYQAIRWAEGPAVPPQEHPPSKIV
                     VIPDGATFQQVAALLERERLIKSRSAFVLFGKSHSADRKIHAGEYELNASMVPAEILS
                     KLLLGQVVLHPFTVPEGLTVSQIADVMAQRGISTREEFMRVASDKALIASLGIKADTL
                     EGYLYPDTYKFPREVKPKEIVVAMVDHLRQVFGADLQVRAQELKMTLHEVLTLASLIE
                     KETGSGGERPEISAVFHNRLKKRIPLQSDPTVIYGLPNFDGNLRKKDLSSPSPYNTYR
                     WVGLPPGPIANPGVQAIRAALYPSNSRALYFVSRNDGTHQFSVTLAEHNEAVEKYQKR
                     PFRRATHPRT"
     gene            complement(7402..7965)
                     /gene="ruvX"
                     /locus_tag="VI359_04425"
     CDS             complement(7402..7965)
                     /gene="ruvX"
                     /locus_tag="VI359_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY6973529.1"
                     /translation="MVSRILALDYGTKRIGVALSDELGWTAQPLETFERRTLDRDIAH
                     IASLVKSHEVGKVVLGLPLQLDGREGPAIEAMREFAAKLEAGLSVPIVRWDERMTTKA
                     AEDLLIAADVSRKKRKGAVDRVAAAILLQSYLASLESPATTEPWTETDADSAPVETSD
                     ETQGHSGSSAGRGHRSRHGRISGDSVG"
BASE COUNT         1731 a         2530 c         2173 g         1649 t
ORIGIN      
        1 cttcgtcgtc tcatacaggc cgccaccgca gtccatgccg acgtttttca tcgctcctga
       61 ggccatcact ttaccgacca tccagattgc cggtccgttg ctgcaccact tgcgcgagag
      121 tctccggctc catcggggtg aatcgttgat cctgacagac catcagggca tcaggtatca
      181 cgtggaggtc acggacatca cgtcacgggc aatccacgct cgtgtcgtcg atcaggaaac
      241 agctccgcct cgctcgaccc cgcagattgt gctcggtcag gctctgttga agggtgagaa
      301 gatggattgg gtgctgcaaa aggcgactga gctcggcgtt gacacaatcg ttccgctcga
      361 aactcatcat agcgtggtga aaccaaaccc ggaacggctt gaacatcagc ggaacagatg
      421 gcgtcgcatc gtgttggaag ctgcacagca atcggaacga tggacgatcc cgcaggtgtc
      481 ggatccgaca acgcttgcac gatttgtcga tcggcagtca ggggccacga tcaaaggaat
      541 actgtcggaa cggagcaagg gttcctccct cacttccctc atgcttccgt gcgagcccca
      601 tcacatgatt gtcctcgcgg tcggaccgga aggcgggtgg acccccgagg aacagcaatt
      661 ggctcgagac tgtggattcg tcgcgttaac actgggggga cggattcttc gagcagaaac
      721 ggcgacactc gccgcactca gcattctcca atcgagactg ggtgaattgg gatagaagag
      781 aatctactga ccggtcgaaa gacgaacgat ggtgcctttt tcaccggtcg cccaaccatg
      841 cagggcgtcg gggaacgaga gcccaaacag cgagactttc gcgacgctcc cccgctccac
      901 ccaggtaaag ccgctatccg ccgtgtggta caccatgcct cgctctccca caatccaccc
      961 ggtccgagga tcggtgaagc ggacccgcag caagtccgtc agcttcgtgc agttcctcgc
     1021 gcaaggaagc gttcgatcca cccatcggct tcctccgtcg gtcgtctgga acatggcgcc
     1081 tgcgttgccg acaacccacc cagtcgaagg atccgagaaa tagacatcga agagggtcac
     1141 cgcaccttgc gtttcttgcg caacccatgt cgcacccccg tcatcggtcg cgaggactgt
     1201 gccgatggca cctaccgccg ttccacgctt gtccgtgacg aactggaccg cgtacaaggc
     1261 cgcagtcgtc gtactagctt ggtcgaccca atgacctccc ccatccatcg tatgcaggat
     1321 cgtcccattg ccgccgacca cccagccctc tgtcggagac acgaatgaga tgccgtacaa
     1381 tggcacctgg gtatcgatcg gctgcggcgc ccacgtttct ccgccattga cgctgtgacg
     1441 aatcgtgccg tttgccccga ccagccatcc gtgtttttcg tcgacaaaca gaactcccgt
     1501 caacagtgtc gtagtcccgc tcgagacctt cttccatttc tgcccgccat cgagggtttt
     1561 aagaacggtt cctcctgatc cgacggccca gcccaggctg ggattacgaa aatacacatt
     1621 catcagcgtc acttccgtcc cggatttttg tgcggtaaag gagaggggag attccccggc
     1681 taccgtggga ggcccaggca ggatggaaat catcagagag gcgaaagtaa aaacactcgc
     1741 gcgacacgca accacggaga gctgtcggcc ccaaccattc atgctaatcg ttggtgctgc
     1801 cttccggcct gacttggaaa aaactttggt cgggaaaacc cttcgcttca atggtgaagg
     1861 tgccggcttc tattgtcagc catttcttcg gcgtacagca cgttccatcg ggcagaatgt
     1921 cccatgaacc acggcgcacg acgagaggtc cggtcgaaag atcgggattg aactcacccg
     1981 gttttccgat cccgaagaga tgacgacggg gaacgcgaac tttgatctcg gtgtcggtcc
     2041 atgaaataac gactgccgcc acattactga acaggacttc tgtccgcgac acattctctt
     2101 caagggtcgg actattccgt ttgtagatgt ccttggtcat acccatcttg ctggcctcag
     2161 tggttttgag gaaattccca aatccactcc cgtggatcgt caccacctca tcgaggcccg
     2221 ctgaatgagg actgtacgag tcgattttcg gcgtcacgat ggtgaaggtt ccggcttctg
     2281 tcgacacaag ctttttctct tcgcaacaac tcccgtccgg tttggggttg gcacttcctc
     2341 tggtcaccac aacaggcccg ctcttcgcgc taaacggaac ccacacatca atccggtcgt
     2401 cgtgccatcg gtagatcacc gctgggacac cgccgatctc aacacgattg tccccggtat
     2461 cgaagtccat gaagttgaac ggcgtggagc cgctttcaga atacgatcca aaatgctcac
     2521 cgctaattct gagtaaggtc ccgatggggg cgctggccgg cgagattccc gtcgccttgg
     2581 gaatctgcac cgtaaaggtt cccacggtcc gatcctgtcc gcgagtcttg aggacgactg
     2641 gtccggtttc cgcattggac ggcacatgga cgacgatcgt gttgtcggac cactgagcca
     2701 cggcagcgga atgaccaccg aagcggacgc catcactagg gtctctggac gcgccaaacc
     2761 cctcgccgaa cagtaccacc ttcgtgccgg tcggaccctg cagcggatcc atccgtaccg
     2821 tgggaatgat ggagatcggc acggaattac tgatcgcata ccgaactggt gcacaacacg
     2881 atccgtctgg aagcgggtcc gacgaggcta accgcaccag gacatcgccg gatttggcat
     2941 tggctggcac ctcgacttca atcttgtcat ctttccatcg gcgaggcctg acgacgactc
     3001 cgccaatgac gacgtcattg actccgaaca ttgtgttggg atcgcgggct ccggccgtga
     3061 tcccaaaatg ttcaccggtg atctcgatcg gatggcccgg ctcgattgca gcaggcgtca
     3121 cggccgtgat cttgggtcga acgagggaaa aggtcccggc agctaatgtt ttctttccga
     3181 tacggacctc taccggaccg gactgagcct gaaaggggac cttgacttcg atcaagtcag
     3241 gttcccaccg ttgaatcagt gccggcacac cggaaaaggt cacacgattg aattgcacag
     3301 agcgaaacga tccaaacccc tttccgccga tcgacaccgt ggcacctggc gttgccgacg
     3361 acggagtcaa ctgcatggtc gtcgacgtgg cggccaacgc tgcgcaagtc atgctccccc
     3421 agaggatggc actactcatc agaagagtga ggagagcctt catgtgtgtg ctcctggata
     3481 tcatcgagaa taacggccct ggcgtggcac gagaacccca cgcaccggat tgaggaaggg
     3541 caaaacgggg gtataacgtg gagcgaggga cggtcgaact ataacaagcg gttttttcgg
     3601 gtgtcaagcc gacaacgccg ccccggctga gcattcgcag tcagctgaac gcttggatca
     3661 agtaatggag agaatcactt cgatttccac aggagcgcgt cgtggcaact ccgccgcccc
     3721 aacggccact cgagcatgac gtccggactc accgaatacc gccactaaga gatccgacgc
     3781 cccgttcacc acctgtggtt gctggacaaa cccttcggca gatgcgacat gtccgacgac
     3841 cttgacgata cggttgatcc gatcaaggct tcccagttcc tgtttggcaa tagcgagggc
     3901 gttcaatacg caaagtttgg ccgcctccat cccttcttcc accgtcagat cccgtcccag
     3961 tttgcctgaa aactccagtt gcccatcccg catcggcaac actccggaca agaacaggag
     4021 atcaccggct cggacggcgg gaacgtacgt cgccaccggc tgcggtggag aaggcagctg
     4081 cagaccaagt gccttcaatt tggcttcata cgacatcatc gctccgaacc tcatcccggt
     4141 cgcaaggcat cgagaaaact gtcccccacc gccaaccgtt cgccacaaag cgcctccacg
     4201 atggtctgac cgagatccgc ggccgtcgat cgagtgccaa gattcacccc ttgagcaagc
     4261 ttcggaccac tcatcagcag cggcacatat tcccgcgtag aaccctgatg gacctttctg
     4321 ggatcgcgcc catgatcacc ggtcaccacg aacaagtccc cgggacgaag ctgttcgaga
     4381 agcgatggca gccgtcgatc aaaatcgtgc aacgccccag ccgacgcggc atcatcggat
     4441 tcgagcacat ccagtccgac ataaatcaac ccgcgaggga cgttcttcag catccccttg
     4501 acgtcttcga ggagcgtcgc ccagtgagcc tggggaatcg tccgcgtcag cccgcgaccg
     4561 ttgaagagat cgccggcttt gccgacgccg gtcaggattt gtccggcacg attcagcacg
     4621 tcgagcattg tcgtaccgga cggctcgatc acaaaatccc gtcgctgatg gctgaaccga
     4681 accgctccct tctctccagt gagtggatgg gcaactacac gcatgatgcc ccatggctct
     4741 ctgagtaatt tcctcgcctc gcggcagagt tgatgcagtg catcaggacg cataacagtt
     4801 gcatgagcgg caaggtgaca ggtccgtcga ccgtctgtcc agacgatggg cgcgcccgag
     4861 gcgagatgat cgttaccata gtcctgaagc atggcggtgg gcgaggccac acgatttccg
     4921 agcatcttcc ggccgctaat ctgttccagg acggcaataa gtggttgggg aaatccgttc
     4981 gggtatgtat gagcggccac agcctgcata tagccgttca tctcccaata ccctcccaac
     5041 gaatcgacat cgctggaaag aaaaccgagc cggccgaaac aaccttccgg ctgagccatc
     5101 acctgcacac ttggaatcgc agtaatatgg ccgagcccca gcgcttccat cgttggaaga
     5161 tgcagtccgc ctgctgcctc ggcgaggtgc acgagcgtgt tggcgctctc atcaccatac
     5221 ttggctgcat ccggcatcgc accgattccg aacccgtcga tcacgataag aatgacgcga
     5281 ttcatcatgc acgcacttcc atcacactgg tgatcagatc aacgctgaaa ccgcacaccg
     5341 ggtcagagga agctgtgacg tcagctggcc attccgtgtt acgaggcact gcgcttcgcc
     5401 acttcaagga gaaacgcatc aagccgttgt ttcgaatctc ccggcacgac cttgaactcg
     5461 acgccgaact cacgtctggt cgcccaacag attcttccca gctcgacaga aatgggtgtc
     5521 ggctgcccag gtaccgtgat ttcgagactc acggtttctc cctccgtggc catgatcccg
     5581 cactcaatgg cacagccccc acgggacaga ttgaacgtcg tcccctgcac ggcctgctcc
     5641 tccaacacac aggccacggg acattggacg tcaaaccgga tatctttccg tattgcaaaa
     5701 atgtcctacc cctttcgcct cgttgccgac cattccgcga gaatcgtcag gctcgcgctc
     5761 gtcccaatgc gatccgcccc cgcctcaagc atggcgagtg ttgtcttcca atctctgata
     5821 ccgccggacg ctttcacttt cgccttaccg gctacggcct gcttcatgag tcgcacgtct
     5881 tcgaccgttg cgccggcagc ggcaaatccc gtcgatgtct tgacatactc gacgccagct
     5941 tcgatggcaa gctggcaggc cgttcgtttc tcctgagcgg tgagatagca cgtttccaat
     6001 atcactttgt gttccgcgtc aggtgtcgcg cgcacaactt cggcaatgtc ttgccgcaca
     6061 taatcatggt cacctgacct cagtcggctc acgttgatga ccatatccag aatccgggcg
     6121 ccgtgcccga cggcttcgag cgcttcagca accttgttcc ttgtcgtatg ccagcccaat
     6181 gggaatccga tcggactccc cactcgaatc tccgattccg cgacggccgc tatcgctata
     6241 tccacataac agggcggaac aaacaccacg agaaatccgg cttgtttggc ctcatggcac
     6301 acacgcagca catcctgttt cgtcgcgtcg ggacggagta tggtgtggtc gatcagcgca
     6361 ggcaggttcc aatcacgagt catcatggaa atctcttata gcaggactgt tcggagagat
     6421 gaagaagaat cacacgagaa gccactgcct ggttacgtac gggggtgcgt cgcgcgtcgg
     6481 aacggtcgct tttgatactt ctccaccgcc tcattgtgtt cggccaacgt cacggagaat
     6541 tgatgcgtcc cgtcgttgcg cgacacaaaa tagagggcgc gcgaattcga gggatagagc
     6601 gccgcgcgaa tcgcctgaac cccgggattc gcaatcggtc ccggcggcaa gccgacccag
     6661 cgatacgtgt tgtacgggct cggactcgag agatccttct tccgaagatt cccgtcgaaa
     6721 ttcggcaggc cgtaaatcac cgtcgggtcg ctttgaagcg gaatacgctt cttcaggcga
     6781 ttgtgaaaga ccgctgagat ctcagggcgc tcccccccgg aaccggtctc tttttcgatg
     6841 agggacgcca gcgtcagcac ctcatgcagc gtcatcttca gttcctgcgc ccgcacctgg
     6901 agatccgctc caaacacctg acgaagatgg tcaaccatgg cgaccacaat ttctttcggt
     6961 ttcacctcac gcggaaactt gtacgtatca ggatagagat atccctccag cgtgtctgct
     7021 ttgatcccca gcgatgcaat gagcgccttg tcgctcgcga cccgcatgaa ctcttcacga
     7081 gtcgagatac cgcgctgcgc catgacatcg gcgatttggg agaccgtcaa cccttccgga
     7141 accgtgaacg gatggagcac cacctggccg agcagcagct tggataagat ttccgccgga
     7201 accatactgg cgttcaattc atattcgccc gcatggatct tccgatcggc ggaatgtgac
     7261 ttaccgaaca ggacgaacgc cgaccggctc ttgatcagtc gctcccgctc aagcaatgcc
     7321 gcaacctgtt ggaacgtcgc gccgtctgga atgacgacga tctttgaggg aggatgttct
     7381 tgtgggggaa cggcaggccc ttcagcccac cgaatcgcct gatatgcggc catgccgaga
     7441 acgatgaccg cgaccagcag aagacccaga atgaccttga gtttcatcgg atgtctctac
     7501 gggcgccgag tccgcatctg tctctgtcca aggctccgtc gttgcgggtg attcgaggct
     7561 tgccaaataa ctttggagca gaatcgcggc tgccacacga tcaactgccc ccttccgctt
     7621 tttccggctg acgtccgcag caatcagaag atcttccgcc gccttcgttg tcatccgctc
     7681 gtcccagcgg acaatcggaa cggaaagacc cgcctccagt ttcgctgcga actcgcgcat
     7741 ggcctcaatc gcaggacctt cgcgtccgtc caattgcaac ggcagcccca acaccacctt
     7801 tcctacctcg tgggacttaa ccagggaggc aatgtgggcg atatcccgat cgagcgtgcg
     7861 ccgctcgaac gtctcgagcg gctgcgccgt ccacccgagt tcatcgctca gggcgacacc
     7921 gatccgcttc gttccgtaat ccaatgcaag gatgcggctc accatgagtt taccgctgga
     7981 gggagttttc gactaggcca aagacttttt ccaacgccgt atccaatcgg gaagggtcct
     8041 tcccccctgc ttgggccatt tccggccgtc cgccgccggt gcc
//
LOCUS       DATDNW010000179         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_441670, whole genome shotgun sequence.
ACCESSION   DATDNW010000179 DATDNW010000000
VERSION     DATDNW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441670"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VI359_04430"
     CDS             <1..740
                     /locus_tag="VI359_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973530.1"
                     /translation="LLVCQELFLTETARLAHVVFPAASYMEKAGTFTNTEGHVQAVRP
                     AIDPVGESRPDWEILSALSVLMGMPLEYGDAKELLKEIRGVIPGYGQLGPAPIPHKVD
                     RATLDQYLAEGYRQNLLSRYTLTPAVRRPEGTVQVGLAQSLFHAGKFSTRSKGLLQVE
                     GRGALRMNPADAARFAVADGDRVRLSNARANVTTFVKVWDRVPEGLAWYPDHFAQEMT
                     QLFECTVDPETKVPSFRIASVSVMKVA"
     gene            789..1859
                     /gene="nuoH"
                     /locus_tag="VI359_04435"
     CDS             789..1859
                     /gene="nuoH"
                     /locus_tag="VI359_04435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY6973531.1"
                     /translation="MTELSLRLAVSLAQIAAVMGIVMLTVMILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPIADGLKLFFKEDIVPAGANKFMFSLAPLLAMVPALIGFAVIPFGP
                     SLTFDVAGIKVQPFVISDINIGILYILAFTSIGAYGIILGGWASNSKYSLLGGLRSAA
                     QVISYELNVGLAIVGVLILAGSLSLVKIAEAQAGGFWHWYLFALPMPQILAFVVYVIS
                     SVAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMVLVSCVAAALFLGGW
                     NAPYPGTIMAQLGMGSIAWIENVAWFTVKVYVFLFLFFWLRATLPRLRYDQLMRFGWK
                     VMLPIALGNIIVTSIAAYFFRQ"
     gene            1872..2450
                     /gene="nuoI"
                     /locus_tag="VI359_04440"
     CDS             1872..2450
                     /gene="nuoI"
                     /locus_tag="VI359_04440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY6973532.1"
                     /translation="MNVNVSQRAPAGRSGFRGWLKTITFYEILVGMKATLSHLLHYKP
                     ITLQYPHEKRNLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEP
                     TKRYSKEYYMDMTRCLFCGMCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGD
                     RSFPVREKRLELQHPNVAFFNIPFKHVPQKPV"
     gene            2520..3038
                     /locus_tag="VI359_04445"
     CDS             2520..3038
                     /locus_tag="VI359_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY6973533.1"
                     /translation="MELAFFFYFALVIIVTSALVVAFRNPIYSALSLLIMFFHVAGLY
                     VTLHAEFLAAVQIIVYAGAILVLYLFVVMLLNMKQDDRYHRQWPIAGLIGGLLLVEAL
                     VLTVARYRVPAVATSEVESVVEQSGNTEAIAEVLYSTYLFPFEVASLILLVAMIGAIL
                     LAKRDLQEAPRS"
     gene            3035..3340
                     /gene="nuoK"
                     /locus_tag="VI359_04450"
     CDS             3035..3340
                     /gene="nuoK"
                     /locus_tag="VI359_04450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY6973534.1"
                     /translation="MTVPMSYYLTLSAIVFLTGLVGVLIRRNIIIILLSVELMLNATN
                     INFVAFSQFFHNVAGQVFVFFALTVAAAEVAVGLAIIIALYRAKSTINVDEFSLLKW"
     gene            3420..>4580
                     /gene="nuoL"
                     /locus_tag="VI359_04455"
     CDS             3420..>4580
                     /gene="nuoL"
                     /locus_tag="VI359_04455"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY6973535.1"
                     /translation="MYALIPLLPFVAFLILGLAGWWIKDRAHLVAVPAVALSWVLSIV
                     AFADVASGSVISVPLYTWLTSGPLDIHIGLSIDRLTAVMLLLVTTVSGLVHVYTIGYM
                     HGEPGYARFFSYIALFTFSMLMLVLADNLLQLYVFWEAVGLCSYLLIGHWYERPSACS
                     AATKAFIVNRVGDFGFLLGLFLAWTSFGSLTYLEIFAQAHQSVNMTLNLLAPLGSQWE
                     VSVVTLVCLLLFMGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVARL
                     APLYNLSPTAMSVVAIIGGLTMVVGATIALTQTDIKRVVAYSTVSQLGYMMMACGLGA
                     YAAGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRQMGGLKDKLPVTYWTFV"
BASE COUNT          829 a         1367 c         1294 g         1090 t
ORIGIN      
        1 acctcttggt ctgccaggaa ctgtttctta cagagaccgc tcggctcgcg cacgtggtct
       61 ttccggcagc ctcgtacatg gaaaaggccg gcacctttac caatacggaa gggcatgtgc
      121 aggcggtacg tcccgcgatc gaccctgttg gcgaaagccg ccccgattgg gagattttgt
      181 cggcgctgtc cgtgctgatg ggcatgccgc tcgaatatgg cgatgccaaa gaattgctca
      241 aagagatccg cggcgtcatc cccggttacg gccaactggg gccggctccg attcctcaca
      301 aagtggaccg ggccacgctc gatcaatatc tagcagaggg ctatcgccaa aacctgttat
      361 cccgctatac tctcaccccc gcagtcaggc ggccagaagg gaccgtgcag gtggggctgg
      421 ctcagagctt gttccatgcg ggaaagttct cgactcgatc gaagggcctg ctccaagtcg
      481 aaggccgcgg cgcattgcgc atgaatccgg ctgatgccgc acggtttgcc gtggccgacg
      541 gtgaccgagt ccgtctttcg aatgcgcggg ctaacgtcac cacgttcgtg aaagtgtggg
      601 accgagtccc ggaagggctg gcgtggtatc cggatcattt tgcacaggag atgacccaac
      661 tgttcgagtg cacggtggat ccggagacga aggtgccatc attccggatc gcgtccgtgt
      721 cggtgatgaa agtggcctag cgcagacagg attgtgaatc cgttcatctg ttatccggga
      781 gtattgtcgt gactgaactc agcttgcgcc tcgctgtttc ccttgcccag atcgccgcgg
      841 tgatgggcat tgtcatgttg actgtgatga ttctaacgct cgccgagcgc aaagttctcg
      901 gctggatgca ggatcgcatg ggccccatgg aagtcggccc ctatggtatt ctccaaccga
      961 tcgcggacgg cctcaagctc ttcttcaagg aggacatcgt tcccgccggc gcgaacaagt
     1021 tcatgttcag cctggcgccg ctcttggcta tggtcccggc gctgattggt ttcgccgtga
     1081 ttccgttcgg tccgagcctg acctttgatg tcgccggcat caaggtccag cccttcgtca
     1141 ttagcgacat caacatcggc atcctgtaca tcctggcctt tacctcgatc ggcgcctacg
     1201 gcatcatcct gggcggttgg gcctccaaca gcaagtattc actcttggga ggattgcggt
     1261 ctgccgccca ggtcatcagc tacgaattga acgtgggact cgccatcgtg ggcgtgctaa
     1321 tcctggccgg ctcgttgagc ctggtaaaga tcgcggaggc gcaggcaggc gggttctggc
     1381 attggtacct cttcgccctc ccgatgcccc agatcctggc cttcgtggtc tacgtgatct
     1441 cctccgttgc agaaaccaat cgtgtgccgt tcgacctgcc cgaagcggaa agcgaactgg
     1501 tcgccggctt ctttactgaa tacagcggta tgcgcttcgc gtttttcttc atcgcggaat
     1561 acgccaacat ggtgctcgtc tcttgcgttg ccgccgcact cttccttgga ggctggaatg
     1621 ctccctatcc cggcacgatc atggcccaat tagggatggg cagcatcgcg tggatcgaga
     1681 atgtggcctg gtttaccgtg aaggtctacg tcttcctgtt cttgttcttt tggctgaggg
     1741 cgaccttgcc gcgactgcgc tacgaccagc tgatgcgctt cgggtggaaa gtcatgctgc
     1801 caatcgcgct tggcaacatc atcgtgactt caatcgccgc atatttcttt cgccagtaac
     1861 ccgaggggca catgaacgtg aacgtctcac aaagggctcc cgcaggacga tccggcttcc
     1921 gcggatggct gaagacgatt acgttctacg aaattctcgt cggtatgaaa gccacgctct
     1981 cccatttact gcactacaaa cccattacgc tgcaataccc gcacgagaaa cggaacttgc
     2041 cggacaacta tcgcggcatg ttggcactcc tccgctatga cgatggcacc gagaagtgtg
     2101 tgggatgcga cttgtgcgag gccgcttgcc cctcacgcgt gatccgtgtg gtcagcgccg
     2161 aggtccctgg agaacccaca aagcggtatt ccaaagaata ctacatggac atgacccgtt
     2221 gcctgttctg cggcatgtgc gtcgacgcct gtccggtgga cgcgctcggc atgacccgcg
     2281 agttcgaatg ggctgtgtac gacaagcgcc agctgcacct gaacaagcag caactgctcg
     2341 ccatcggcga tcgatctttc ccggttcggg aaaagcggtt ggagctgcag catccgaacg
     2401 tggcgttctt caatatcccg tttaaacacg tgccgcaaaa gccggtctga acacaccgag
     2461 gccggatatc gtgctgcgcg caacatgcgc acagggtgga atcggactga gtcatctgga
     2521 tggaactggc attcttcttt tacttcgccc ttgtaatcat cgtcacgtcg gccctcgtgg
     2581 tcgcgttccg gaatcccatc tatagcgcgc tctccctcct tattatgttt tttcacgtcg
     2641 cggggctgta cgtcactctc catgcggagt tcctcgccgc cgtgcagatc atcgtgtatg
     2701 ccggtgccat tctggtgttg tatctcttcg tcgtcatgct gctgaacatg aagcaagacg
     2761 accggtacca tcgacagtgg cccatcgcag gattgatcgg gggactgctc cttgtggaag
     2821 cgctcgtctt gactgttgca cggtaccgcg taccggccgt ggcaacgtca gaagtcgaat
     2881 cggtggttga gcagagcggc aacaccgaag cgatcgctga agtcctgtac tcgacgtacc
     2941 tgttcccttt cgaagtcgcg tcgttgatcc tgctcgtggc catgatcggc gccattctct
     3001 tagccaaacg cgacctccag gaggccccga gatcatgacc gttccaatgt cctactatct
     3061 cacgctcagc gccatcgtgt ttctgaccgg actcgtcggc gtgttgatcc gccggaatat
     3121 catcatcatc ttgctctcgg tggaattgat gttgaatgcc accaacatca atttcgtcgc
     3181 cttttcccag ttctttcata acgtcgcggg acaggtcttc gtcttcttcg ccttgacggt
     3241 ggcggcggcg gaagtggccg taggcctggc catcatcatc gcgctttacc gggcaaagtc
     3301 cacgatcaat gtagacgagt tcagtctcct gaagtggtga caatcaacat gttgagaact
     3361 gctagaaagc agaaaatcac ctcagcacgc agcacgccgc acgcagtcct gggatgatca
     3421 tgtacgcctt gatccctctc cttccatttg tcgcgttttt gatcctcggt ttggccgggt
     3481 ggtggatcaa ggatcgcgca catctggtgg ccgtccccgc tgtcgccttg tcatgggtcc
     3541 tctcgatcgt cgcgtttgcc gatgtcgcct cgggatcggt catctctgtg cctctgtaca
     3601 cgtggctcac ctccggcccc ctcgatattc acatcggatt gtcgatcgat cggctgacgg
     3661 ccgtcatgtt gctattggtg acgacggtga gcggcctcgt ccacgtctac acgatcgggt
     3721 acatgcatgg ggaaccgggc tatgcgcggt ttttcagcta cattgccctg tttaccttct
     3781 ccatgctcat gctcgtcctg gcggacaacc tgcttcagct gtacgtgttc tgggaagcgg
     3841 tcgggctctg ctcgtatctc ctgatcggcc attggtacga acgaccatcg gcctgttccg
     3901 cggcgacaaa agcgtttatc gtgaaccggg tcggcgattt cggatttctc ctcggtctgt
     3961 tcctggcttg gacatcgttc ggctcgctga cgtatctcga gatctttgcc caggctcacc
     4021 aatccgtgaa catgacgctg aatctgctcg ccccgctggg cagccagtgg gaggtctcag
     4081 tcgtcacgct ggtgtgtctc ttgttgttca tgggagccgt cggcaaatcg gcgcaggtgc
     4141 cgttacacgt ctggcttcct gatgccatgg aaggtcccac tccgatctcc gctctcatcc
     4201 atgcagcgac aatggtcacg gccggtgtct ttatggtggc ccgcttggcc cccctctaca
     4261 acctttcccc caccgccatg tcggttgtgg cgatcatcgg cggcctgact atggtcgtgg
     4321 gagccaccat cgccctgaca cagaccgaca tcaagcgggt ggtcgcttac tccaccgtga
     4381 gccagctcgg ttacatgatg atggcctgtg gtctgggtgc gtacgcggcc ggaatgtatc
     4441 acctcctgac ccacggagcc ttcaaagccc tgctgttctt aggctgcggc tccgtgatca
     4501 tcgcgctcca tcatgagcag gacatgcggc agatgggagg cctcaaggac aaactgcctg
     4561 tgacctactg gaccttcgtc
//
LOCUS       DATDNW010000180         7005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_225540, whole genome shotgun sequence.
ACCESSION   DATDNW010000180 DATDNW010000000
VERSION     DATDNW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225540"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..214
                     /gene="rpmF"
                     /locus_tag="VI359_04460"
     CDS             29..214
                     /gene="rpmF"
                     /locus_tag="VI359_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY6973536.1"
                     /translation="MPNPKHKHSRARRDKRRTAKLRMTPPGMSVCPQCHELKLPHYVC
                     LNCGTYKGKAVIQVEET"
     gene            427..1449
                     /gene="plsX"
                     /locus_tag="VI359_04465"
     CDS             427..1449
                     /gene="plsX"
                     /locus_tag="VI359_04465"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY6973537.1"
                     /translation="MKIALDAMGGDHGSAPAIEGAAQAVREFDVDVILVGDEAVLSSE
                     CRRLGCTDARLTIRHAPQVVEMHESPAAVARKKRDSSIWIATELVKSGEAQAVVSPGN
                     TGASMVASFFVLGLTKGVERPAIATSLPTLTGTAIMLDVGANVDCSAKHLEQFAIMGH
                     EYGKYLFGKPNPRVGLLSIGEEDSKGNEVTKEAFKLLKSSPLNFIGNVEGRDVYSGEA
                     DVVVCDGFIGNVALKISEGVADTIKKLLLKEISGSFFGRLAYPLIAGPLLNLKRKIDY
                     AEFGGAPLLGVNGITMICHGRSSAKAIKNAIRRAKGLAESRVDDLIQRDIEENLSRAR
                     TEEPTA"
     gene            1446..2420
                     /locus_tag="VI359_04470"
     CDS             1446..2420
                     /locus_tag="VI359_04470"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY6973538.1"
                     /translation="MKARIRGTGSYVPAKVLTNVDLERMVATSDEWITERTGIRERRL
                     VGAGEACSDLAVKAAERALKAAGVSAAELDLILLATCTGDFPLPATACLIQHQLGATR
                     AAACDLSAACCGFVYALSVADAYIKTGLRHVLVIGSEVMSAIMDWTDRNTCILFGDGA
                     GAAVVSASDGDRGILSCHLRSDGALCDLITVPGGGSRNPPSEKIIAERMQFIKMKGNE
                     TFKVAVRALEDIARETLAANNLTVNDLDLYVPHQANVRILKSVAGRLGLPLEKMMLNL
                     DRYGNTSAASIPIALDEAVQAGRIKEGSLVMVGAFGAGLTWASALIRW"
     gene            2641..3579
                     /gene="fabD"
                     /locus_tag="VI359_04475"
     CDS             2641..3579
                     /gene="fabD"
                     /locus_tag="VI359_04475"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY6973539.1"
                     /translation="MPSHIAFVFPGQGSQSVGMGRALVDAQPALRAVYDEASSVLGYD
                     VAALCFEGPAERLNLTEYTQPALLVSSVAALRALDPLGLIPAAVAGHSLGEYSALVAA
                     GGLPYRDAVAVVQKRGRYMAEAVTPGTGLVAALLGLTADVVKEVCREASSIGIVAAAN
                     FNSPGQVVIAGEKAAVERAVELAKTRGCKKAIPLPVSVPVHTPLMQKAADRLAADLGM
                     VNWSDLKVPLINNAEAKALSKAEEIKASLIRQLPSSVLWEDSIKTMAAMGITRFVEVG
                     PGAVLSGLIKRILPQATTWTVNDPKSLEATRVALAA"
     gene            3600..4343
                     /gene="fabG"
                     /locus_tag="VI359_04480"
     CDS             3600..4343
                     /gene="fabG"
                     /locus_tag="VI359_04480"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY6973540.1"
                     /translation="MSLQGKVAIVTGAAQGIGRAIAEALAQAGADIAVADLDPGRSAD
                     AVAAVEKIGRKALNIKVNVADANDTKAMAEQVMKAWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFNCTKAVLQPMTKQRYGRIVNIASIVGVMGNVGQANYAASKAA
                     VIGFTKTVAREYASRNVTVNAVAPGFIDTAMTQGLPVEVKETLQKQIPLGRLGTPADI
                     AAAVRFLVSDDAAYVTGHVLHVNGGMLMS"
     gene            4424..4657
                     /gene="acpP"
                     /locus_tag="VI359_04485"
     CDS             4424..4657
                     /gene="acpP"
                     /locus_tag="VI359_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY6973541.1"
                     /translation="MATVDERVKKIIAEQLGVEEDEVTPEASFVEDLGADSLDTVELV
                     MALEEEFGIEIPDEDAEKILTVGKALEYIKEKA"
     gene            4672..5922
                     /gene="fabF"
                     /locus_tag="VI359_04490"
     CDS             4672..5922
                     /gene="fabF"
                     /locus_tag="VI359_04490"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY6973542.1"
                     /translation="MSERSTRRIVVTGLGVVTPLGTGVEKTWKALCAGESGIGRITKF
                     DPTGYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGAAQMAADDAGLKVAPEEAT
                     KVGVYIGSGIGGLGSIEHYHKILLEKGPGRVSPFFIPMTIINLASGQVAIRLGAKGPN
                     SCAVTACATGNHCIGDAYRLIQRGDADVMLAGGAEAAITTLGVAGFAASKALSFRNDE
                     PTKASRPFDRDRDGFVLGEGAGVVVLEELEHARRRQARMYAEVIGYGMNSDAYHITAP
                     SEEGEGAVRCMELALKDAAIEKDQIDYINAHGTSTMADAIETKAIKQVFGDRAYRIPV
                     SSTKSMTGHLLGAAGGIEAVFSVLALYHGILPPTINLDHPDPECDLDYVPHKARQVRV
                     NVALSNSFGFGGVNACLLFKKLHA"
     gene            6073..6780
                     /gene="rnc"
                     /locus_tag="VI359_04495"
     CDS             6073..6780
                     /gene="rnc"
                     /locus_tag="VI359_04495"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY6973543.1"
                     /translation="MIPVPSPESLQATLDYRFQDQALLDEALTHKSYVNEQKGVVCQD
                     NERLEFLGDAVLSLVISEYLASRLPDSTEGALSKLKAQLVSESSLERVARRLQLGGYL
                     KLGRGEELSHGREKSSLLADAVEALLAAVYLDGGYEASRRVTLHIFAAEVSQVDLRVH
                     NAGADDYKTQFQEWCQKRHDILPHYKTIRESGPDHDKCFDVEVTIRGEVVGAGSGRSK
                     KEAEQQAARQALERARM"
BASE COUNT         1541 a         1835 c         2201 g         1428 t
ORIGIN      
        1 ccgtgttcag ccgtcgtaag gagcagccat gccaaatccg aaacataaac attcacgagc
       61 caggcgcgat aagcggcgaa cggccaagtt gcgcatgaca ccgccgggca tgtcggtatg
      121 cccgcaatgc cacgaattga aactgccgca ttacgtctgc ctgaattgcg ggacctacaa
      181 gggcaaggcg gtcatccaag tcgaagaaac atgaagagga tggtggggaa gcccttgttg
      241 accaccccgt agatgggaag tgagatgggc ggcaaggtgc gttttgcgtc gccaggaacc
      301 tgttttcacc atcctgttgt gcaacccgat tttctgctgt aagcttccag cccaagtgac
      361 caggccacga atgtcatccg cgcaccccta ttgtccgcac gattccctgt gagtgctcaa
      421 tctcgcatga aaattgccct ggacgcgatg ggtggagacc atgggtcggc tcccgccatc
      481 gaaggcgctg ctcaagctgt cagagaattc gatgtcgacg tcattctcgt cggagatgaa
      541 gcggtcttga gcagcgaatg ccgccgtctt ggctgtaccg atgcccgtct cacgattcgc
      601 catgcgccgc aagtggtcga aatgcatgaa tcgccggcgg cggtcgcgcg gaaaaaaaga
      661 gattcttcaa tctggatcgc cacagaattg gtgaagagcg gggaagccca agctgtcgtg
      721 agtccgggca acacgggtgc cagcatggtg gcctcgttct tcgtgttagg cctgacgaag
      781 ggcgtcgagc gcccggccat tgcgacgagt cttcccacgc tgaccggcac tgccatcatg
      841 ctggatgtcg gcgccaacgt ggattgctct gccaaacatc tcgaacagtt cgccatcatg
      901 ggtcacgagt atgggaaata cctcttcggc aaaccgaatc cccgcgtggg acttttgagc
      961 atcggcgaag aggatagcaa gggcaacgaa gtcaccaaag aagcattcaa actcttgaaa
     1021 tcaagtccgc tcaatttcat cggcaacgtg gagggccggg atgtctatag cggcgaggcg
     1081 gacgtggtgg tgtgcgacgg gttcatcgga aatgtggcgc tcaagatctc tgaaggcgtg
     1141 gccgatacca tcaagaaact cttgctcaag gaaatttccg gctcgttctt cggccggttg
     1201 gcctatccgc ttattgctgg ccccctgttg aatttgaagc gaaagatcga ttacgcagaa
     1261 ttcggcggtg cgccgttgct cggcgtgaac gggattacga tgatttgtca cggtcgatcg
     1321 tccgccaagg caatcaagaa tgccatccgc cgtgcgaaag ggttagcgga gagccgcgtc
     1381 gacgacttga ttcaacgcga catcgaggaa aacctgagcc gcgctcgaac tgaggagcca
     1441 acggcatgaa ggcgcgcatc aggggaaccg ggtcctacgt tcctgccaag gtgttgacga
     1501 atgtcgatct cgagcgcatg gtggcaacgt cggatgagtg gatcaccgaa cgaaccggga
     1561 ttcgtgagcg tcgtctcgtc ggcgccggcg aagcttgttc tgatcttgca gtcaaagcag
     1621 ctgaacgagc gctgaaggct gccggcgtga gtgccgccga attggatctc atcctcctgg
     1681 caacctgtac gggcgacttt ccgctgccgg ccactgcctg tctcattcaa catcaactcg
     1741 gtgcaacccg cgccgccgcc tgtgacctgt cggctgcttg ttgcggattt gtgtacgccc
     1801 tgtcggtggc ggatgcctat atcaagaccg gactgcgtca tgtgttggtc atcgggtccg
     1861 aagtcatgtc ggcgattatg gactggaccg accgcaatac ctgcatcctg ttcggcgacg
     1921 gagccggtgc ggcagtcgtg agtgccagcg atggggaccg aggcattctc tcgtgtcatc
     1981 tgagatccga cggggcgttg tgtgacttga tcacggtacc gggcggggga tcgcgaaatc
     2041 ctccatccga gaagatcatt gcggaacgga tgcagttcat caaaatgaag ggtaacgaga
     2101 cgttcaaagt ggcggtacgt gcgctggaag acatcgcccg tgaaactttg gctgcgaata
     2161 acctcaccgt gaacgacctt gatctatatg tcccgcatca ggcgaatgtg cggattctca
     2221 agtctgtcgc gggccggctg ggccttccgc tcgaaaaaat gatgctcaat ctcgatcgct
     2281 atggaaatac ctcggcggcg tcgattccga tcgcgcttga cgaagcggtg caagccggca
     2341 ggattaagga agggtcgctg gtcatggtgg gcgcgtttgg agcgggattg acgtgggcct
     2401 ctgcgttgat tcgttggtaa ggagtaagcc ggtcggcggt cagttgtcag cgtgatgaat
     2461 taagggattc gccgatgtct cgcaaagctt ttttcccgtt gacattcaaa gcaaattcga
     2521 cgatatctaa ccccgctagg ataggccctc agcctgcccc ggcgcttcac aaagcacaca
     2581 aaaggataac gagtcgcctc gcacaattgc ctctgtagca tggcggctct ccaaaccact
     2641 atgccgtccc acattgcctt cgtttttcca ggccaggggt cccagtctgt cgggatgggc
     2701 agggcgttgg tcgacgcgca accagcgctt cgtgcggtct atgacgaagc ctcatcggtg
     2761 ctcgggtacg atgtggccgc gctgtgtttt gaaggacctg cagaacgatt gaatctcacc
     2821 gagtacacgc aaccagcctt gttggtgagc agtgtcgcgg cgctacgtgc gttggacccg
     2881 ttgggtctca ttcccgcggc tgtggcaggg catagtctcg gagaatactc ggcgctggtg
     2941 gcagccggag gcctccccta tcgcgacgct gtggcggtgg tgcagaagcg gggacgctat
     3001 atggcggagg ccgtgacacc gggaaccggg cttgtggcgg cgcttctcgg cctgacggct
     3061 gacgtggtca aagaggtctg tcgcgaagcc tcttcaatcg ggattgtggc agcggcgaat
     3121 tttaattctc ccggtcaagt cgtgatcgcg ggtgagaagg ctgcggttga acgagccgtc
     3181 gaactcgcga aaaccagagg ctgcaaaaag gccattccct taccggtgag tgttccggtc
     3241 catactccgt tgatgcaaaa agctgcggat cgattggccg ctgaccttgg gatggtaaac
     3301 tggtcggatc tcaaggttcc gttgatcaat aatgcggaag cgaaggcctt gtccaaggcc
     3361 gaagaaatca aggcgtctct catccggcag cttccgtctt cggtactctg ggaagactcg
     3421 atcaagacga tggcagccat gggaatcacg aggttcgtcg aggtcggacc gggagcagtc
     3481 ttgtcgggac tgatcaagcg tattttgccc caagctacca catggaccgt caacgatccc
     3541 aaatcgcttg aggcaacgcg cgtcgcgctt gctgcgtgag gggtgtgagg agcgaacgga
     3601 tgtcattgca aggcaaagtt gcaatagtca caggggcagc acaaggaatc ggacgcgcga
     3661 tcgccgaggc gctcgcacag gccggcgccg atattgcggt ggccgatctt gatccgggac
     3721 ggtcggccga cgcggtggca gcggtggaga aaatcgggcg caaggcgctc aacatcaagg
     3781 tgaacgtcgc cgacgccaac gacaccaagg caatggcgga gcaggtcatg aaggcctggg
     3841 gaaagatcga catcctcgtc aacaatgctg ggatcacccg tgatgggtta ctgctccgga
     3901 tgaaagaaga ggactggaat ttggtcttgc aagtgaattt gaacggcacg ttcaactgca
     3961 caaaggcggt cttgcagccg atgaccaagc agcggtatgg tcgcatcgtc aatatcgcgt
     4021 ccatcgtcgg ggtaatgggc aatgttggtc aggccaacta cgcggcctcg aaggcggcag
     4081 tgatcggatt taccaagacg gtggcgcgtg agtatgcaag ccgcaacgtg acggtcaatg
     4141 cggtggcgcc cggattcatc gatactgcga tgactcaggg gttgccggtg gaggtgaagg
     4201 agactctgca aaaacagatt ccgttgggac ggctcggtac gcctgcggat atcgccgcgg
     4261 ctgtacggtt tcttgtctcg gatgatgcgg cgtacgtgac cggacatgtg ctgcacgtca
     4321 acggcggcat gctcatgtcg tgagacggtg acaggcttga tgccgcagcc gggatgctgc
     4381 ggggaggatt caggatcatg atgggaagga ggtcaggata tcgatggcta cggtagatga
     4441 acgagtcaag aaaatcatcg cagagcaatt gggcgtagag gaagacgagg tgactccgga
     4501 agcaagtttc gtggaggacc ttggggctga ttcactggat acggtcgagc tcgtgatggc
     4561 gctcgaagag gaattcggga ttgaaattcc ggacgaggat gccgagaaga tcctcactgt
     4621 cgggaaggcg ttagaataca tcaaggaaaa agcataggca ggttcggaac catgagcgaa
     4681 cgttcaacca ggcgcattgt cgtgaccggt ctcggagttg tcacaccgct cgggaccggc
     4741 gtggagaaaa cgtggaaggc cctgtgtgcg ggcgagtccg gcatcgggcg cattacgaag
     4801 ttcgatccca cgggatacga cgctcaaatt gccggcgaag tcaaagattt cgaccccgcc
     4861 caattcatcg agaaaaaaga aatcaagaaa atggacacgt tcattcacta tgccgtcgga
     4921 gcggcccaga tggcggcgga cgatgcgggg ctaaaagttg cgccggaaga ggcgacaaaa
     4981 gtcggcgtgt acatcgggtc cggcatcggc gggctcggct cgatcgagca ttaccacaag
     5041 atcctccttg aaaaggggcc cgggcgcgtc tcgccgtttt tcattcccat gaccatcatc
     5101 aacctggctt ccggtcaagt agccattcga ttaggagcga aggggccgaa ttcctgcgcg
     5161 gtgaccgcgt gcgccacagg gaaccattgc atcggcgatg cctaccggtt gattcagcgt
     5221 ggggatgcgg acgtgatgct ggcgggcgga gcggaggcag ccatcaccac attgggggta
     5281 gcggggtttg ccgcgtcgaa agccctgtcg ttccgaaacg atgaaccgac gaaagccagc
     5341 cggcccttcg acagggatcg cgacggcttt gtactcggcg agggagcggg cgtggtggtg
     5401 ctggaagaac tcgagcatgc gcgccgacga caggcgagga tgtatgccga ggtcatcggt
     5461 tatggaatga acagcgacgc ctatcacatt acggcgccat ccgaagaagg cgagggggcg
     5521 gttcgatgca tggagttggc attgaaggat gcggcgatag aaaaagatca gatcgactat
     5581 atcaatgctc atggcacgtc gaccatggcc gatgccattg agaccaaagc catcaagcag
     5641 gtctttggag accgggccta tcgcattccg gtcagctcaa cgaagtcgat gaccggccat
     5701 ttgctgggtg cagccggcgg gatcgaggcg gtgttcagcg tcttagctct ttatcacggc
     5761 atccttcctc ccacgatcaa cttggatcac ccggatcccg agtgtgacct cgactatgtt
     5821 ccccacaagg ccaggcaggt acgcgtgaat gtcgctctgt cgaattcatt cggattcggc
     5881 ggcgtcaatg cctgtctgct cttcaagaaa ctgcatgcct agcaggttgt tgaaaacacc
     5941 accctctttc cctctccgcc cccggtatcg tcgtacctgc tcactcagag tttgccgaga
     6001 caggctccgt tctgctagga cgcgcctcga cacgcctcac acattgttca gagactcgtc
     6061 gtacgtgccg tcatgattcc ggtcccttcc cctgaatctc tccaggcgac gttagactat
     6121 cgttttcagg atcaagcgct actcgatgaa gccttgacgc ataagtcgta tgtcaatgaa
     6181 cagaagggcg tcgtgtgcca agacaacgag cgactggagt ttctcggtga tgcagtactg
     6241 tcgctcgtga tcagtgagta tcttgcctct cgcttgcccg actcgaccga gggagccctg
     6301 tcgaagctga aggcccagct cgtgagcgaa tcgtcgttgg agcgggttgc ccgacgattg
     6361 caattgggcg gatatctcaa gcttggacgg ggagaagaat tatcgcatgg cagagagaag
     6421 tcgtcgctct tggccgacgc ggtcgaagct ctcctggcag cggtatacct ggatggaggt
     6481 tatgaagcca gccgtcgggt gacgttgcac atttttgccg cggaagtgag tcaggtcgac
     6541 cttcgtgtgc acaatgccgg agccgacgac tacaaaacgc agttccaaga atggtgccag
     6601 aaacgacatg acatcttgcc acactataag acgattcggg aatcaggccc ggaccacgac
     6661 aagtgcttcg atgtggaagt gaccatccgc ggcgaggtcg ttggcgccgg gtcagggcga
     6721 agcaagaaag aagcggagca acaggctgcc aggcaagcgc tcgaacgagc caggatgtga
     6781 cgctcgagca tggtaagatg atctccagca tcgccaattt gagggaagga ggatgttcat
     6841 gcagagacga acgatgctca gcgtgtgtgt tgtcatggtg tgcgtcaccg gagtcttgtt
     6901 gctgaatacc ggattgctgg gtgcggctga caagatgtcg gcttcgaagg cccccaaagc
     6961 gattatcaag accaagtttg gtgatatgga agtgacgttc ttccc
//
LOCUS       DATDNW010000181         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_22655,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000181 DATDNW010000000
VERSION     DATDNW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22655"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="uvrA"
                     /locus_tag="VI359_04500"
     CDS             <1..1458
                     /gene="uvrA"
                     /locus_tag="VI359_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HEY6973544.1"
                     /translation="DILRQLNAKLRFLRRVGLGYLTLTRQTKTLSGGEAQRVSLANQL
                     GSGLVGTLYVLDEPTIGLHARDTDLLAGILKDLAAVGNTVVVVEHDRHMIESADYIVE
                     LGPGSGEKGGEVVCAASTKEFLSDRRSVTARYLRGEDSIPLPRSRRSGTGKVLVLAGA
                     SEHNLKDLVVRIPLGMLVCVTGVSGSGKSTLIEDTLYRAVARAFRVGSLPMGRFKAIK
                     GLEHLKGVRLIDQQPIGRTPRSNPITYLKAFDEIRSIFASEREALRQGLTPSHFSFNA
                     SDGRCDRCEGSGVEKLEMYFFEDIYVPCETCEGKRFKPQVLAIRHRGKTIADVLNLTV
                     DDALAFFSNAPRLSARLHLLSSLGLGYLRLGQSATTLSGGEAQRLKIAAELRDSTAKD
                     VLYIMDEPTTGLHFDDIKKLLSVLHKLVNAGNTMVVVEHNLDVIKTADWIIDLGPEGG
                     AAGGQIVAEGRPEQVAKVAVSYTGTFLCQAFEGQR"
     gene            complement(1475..>2452)
                     /locus_tag="VI359_04505"
     CDS             complement(1475..>2452)
                     /locus_tag="VI359_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY6973545.1"
                     /translation="PFSILIGGCVIYPFTNVAMEHEREYPHHVVLVDDELCAWGPPAA
                     ILQRRRVCETVGIPHARNGGMPGRPEPVVTGLGPINRETIDHYRTMAVMLFEDAAGVQ
                     GSGSRVAGIVTTLLLDLDINAVERAKLDEVLKEQVIQLTHADDANVLKVGKLVGAQAI
                     FVGGVQQWERHEQARTNSVSLSLRMIDVETGQLLFSGEGHLADPTSDDPEGSARLIVH
                     RILARFGSQTGLLGSGRIGVNWELQESAGFRFYLVRELRSGLPAEKAGLKVGDRIVAC
                     NGAALATVKTERDAKQLCQVEAGQTLQLDVRRGNAAMDIVVLAERRPGL"
BASE COUNT          522 a          742 c          681 g          507 t
ORIGIN      
        1 gacattctgc gacagctcaa cgccaagctg aggtttctcc gacgggttgg gttgggatat
       61 ctcaccctca cgcggcagac aaaaaccctg tccggcggcg aggcccagcg agtgtcccta
      121 gccaaccaac tcgggtccgg attggttggg acgctctacg tcctcgacga acccacgatc
      181 ggacttcatg cgcgagacac agacttactg gctggcatcc tcaaagacct ggccgcggtg
      241 ggaaataccg tcgttgtcgt tgagcacgac cgtcacatga tcgagtcggc cgattacatc
      301 gtcgaattgg gacccggttc tggagaaaag ggcggtgaag tcgtctgtgc agcgtcaaca
      361 aaggaatttc tttcagatcg ccggtctgtc accgctcggt acctgcgcgg cgaagattcc
      421 attcccctgc cgagatcacg ccgttcggga accggcaagg tgcttgtgct ggcgggcgcc
      481 agcgaacaca atctgaaaga cctcgtcgtg cgtattcccc tcggcatgtt ggtctgcgtg
      541 actggtgtct ccggatccgg caaaagtacg ttgatcgaag acacgctcta ccgagcagta
      601 gcgcgcgcct tccgcgttgg ctcacttccg atggggcgat tcaaagccat taaaggattg
      661 gaacacctta aaggcgtccg actgatcgat cagcaaccga tcggacgaac gcccagatcg
      721 aatcccatca cctatctcaa ggcgttcgac gagattcggt cgatcttcgc ctcggaacgg
      781 gaggcgcttc gtcaggggct cacgcccagc catttctcgt tcaatgcctc ggacggtcga
      841 tgcgatcgtt gcgaaggcag cggagtcgag aaattagaga tgtacttttt cgaagacatc
      901 tatgtcccct gtgagacctg cgaggggaag cggttcaaac cgcaagtcct tgctatccga
      961 catcgcggga aaaccattgc cgatgtcctc aacctgacgg tcgatgacgc cctcgccttc
     1021 ttttccaacg caccaagact ttccgcgaga ctccatctcc tctcctcact cgggctcggc
     1081 tatctaagac tgggtcagtc tgccaccaca ctgtccggcg gcgaggctca acgtttgaag
     1141 atcgcggcgg aacttaggga ttccacggcc aaggatgtcc tgtacatcat ggacgaaccg
     1201 acgaccggat tgcactttga tgacatcaag aagctcttgt ccgtcctcca taagctcgtc
     1261 aatgcaggca atacgatggt tgtggtcgaa cacaacctcg acgtcatcaa gaccgcagat
     1321 tggattattg atttaggccc tgagggagga gcagcaggtg ggcagattgt cgcggaggga
     1381 cggccggaac aggtagcaaa ggtggccgtg tcctacacag gaacttttct gtgtcaggcc
     1441 tttgaaggcc aaagatgaga cggcctgacg ccacttacag tcctggtcta cgctctgcaa
     1501 gaacaacaat atccatcgcg gcatttccgc gacgcacgtc gagctggaga gtttgccccg
     1561 cctcgacctg gcagagttgt ttggcgtctc gttccgtttt taccgtcgcg agcgcggctc
     1621 cattgcaggc cacgatacga tcaccgacct tgaggccggc cttttcagcc ggcaggccgc
     1681 tccgaagttc ccggaccaga taaaaccgaa acccggccga ttcttgcagt tcccaattca
     1741 ccccgatgcg ccccgatccc aacaaacccg tctgtgagcc gaaacgggcg aggatacgat
     1801 ggacgattaa tctcgccgat ccttccgggt catcgctcgt cggatcagcc aaatggccct
     1861 cgccgctaaa gagcaactga ccggtttcca cgtcgatcat ccggagcgag agggacacgc
     1921 tgttggttct ggcctgctca tggcgttccc actgctgcac gcctccgacg aaaatggctt
     1981 gcgcacccac cagcttgccg acttttaaca cgttggcatc atccgcatgt gttaactgaa
     2041 taacttgttc tttgagcacc tcgtccagct tggcacgttc aaccgcgttg atatcaagat
     2101 cgagcaggag ggtcgtgacg attcccgcca cccgcgaccc ggacccctgc acaccggcag
     2161 cgtcttcgaa cagcatgacg gccatggtgc gatagtgatc gatggtctcg cgattgatag
     2221 ggccgaggcc cgtcacgacc ggctcgggcc gtcccggcat cccgccgttt cgtgcgtgcg
     2281 gaattcccac cgtttcgcaa acccgtcgtc gctgaaggat agcggcgggc ggtccccagg
     2341 cacagagttc gtcgtccacg agcaccacgt ggtgcggata ttcacgctca tgctccatgg
     2401 cgacgttcgt aaagggatag atcacacacc cgccgatcaa gatcgaaaat gg
//
LOCUS       DATDNW010000182         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_706207, whole genome shotgun sequence.
ACCESSION   DATDNW010000182 DATDNW010000000
VERSION     DATDNW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706207"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2631)
                     /locus_tag="VI359_04510"
     CDS             complement(<1..2631)
                     /locus_tag="VI359_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973546.1"
                     /translation="MDAGSPELTPDVKRKYKFDSRFLDDMVRASWDTAFTYVAKGAIV
                     IAQRYSGEAGARRLREQGYAPEMIEMMKGAGVRCFKHRAGMPVLGIIGKMFNTRFNGG
                     VNALLDSWIRKNGPDQAQGGRYWSNYTWHGDQNPSNPWWCGAQASDIDLSDMRFSKLN
                     TSWGKNFVENKMPEAHWKLECIERGARVVVITPEYNPTAYRADYWIPVRPNADCAIFL
                     GALKIIVDENMHDMDFLKQFTDAPLLMRTDTLQYLDPRDVIADYKFPDFSKSYSGRIQ
                     SLKPEQIERLGGMMVWDVNKKQAVPLHREQVGWHMQSSGIDPAMTGTYRVKLLNGRET
                     DVMPIWQGYLIHFQDYDLDTSHQITRCPKDLLVRWARDSGTIKPAAIHNGEGTNHYFH
                     MTENSRAAAMVLIVTGNVGKFGTGQHTWAGNYKAGIWNSTPWSGAGIAVHTGEDPFNL
                     TLDPNAHGKEIKTKSYYYGEEVAYWNHGDTALIVNTPKYGRKVFTGKTHMPSPTKVRW
                     VTNVNVLNNSKHHYDMVKNVDPNIEMIVTQDIEMTSDVNHADVAFACNSWMEFTYPEM
                     TATVSNPWIQIWKGGIRPLYDTRNDGDTFAGVAAKLSDITGDKRMRDVYHFVYLNRVD
                     VYVQRVLDASCTCYGYSADVMLKSEKGWFSMGRTYPRHPFWEEVNESKPMWTRSGRFE
                     NYRIEPEAIEYGENFIVHREGPEATPYLPNAIMSTNPFVRPDDYGIPITAQHHDDKHV
                     RNIKLPWNDIKRNANPLWEKGYQFYCVTPKTRHRVHSQWSVNDWVQIYESNFGDPYRM
                     DKRTPGVGEHQLHINPQAAKDRGINDGDYVYVDGNPVDRPYRGWKPSDPFYKVARLMI
                     RAKYNPAYPYHVTMAKHAPYV"
BASE COUNT          455 a          807 c          771 g          617 t
ORIGIN      
        1 cacgtacggg gcatgtttcg ccatcgtcac gtggtacgga taggccgggt tgtacttggc
       61 ccggatcatc aggcgcgcca ccttgtagaa cggatccgag ggtttccagc cgcgatatgg
      121 ccggtccacc gggttgccgt cgacatagac gtagtcgccg tcgttgatcc cgcggtcctt
      181 ggctgcctgg gggttgatgt gcaactggtg ctcgccgacg cccggtgtcc gtttgtccat
      241 gcggtacgga tcgccgaagt tcgactcgta aatctgcacc cagtcgttca ccgaccactg
      301 gctgtgcacc cggtggcggg tcttcggggt gacgcagtag aactggtacc ccttctccca
      361 caacgggttc gcgttccgct tgatgtcatt ccacggcagc ttgatattcc ggacgtgctt
      421 gtcgtcatgg tgctgtgccg tgatcggaat cccgtagtca tccggccgca cgaaggggtt
      481 cgtcgacatg atggcgttcg ggaggtacgg cgtggcctcc gggccttccc ggtgcacgat
      541 gaagttttcg ccgtattcga tggcttccgg ctcgatccga taattctcaa agcggccgga
      601 gcgcgtccac atcggcttgc tctcgttgac ctcttcccag aacggatgtc ggggataggt
      661 ccggcccatg gaaaaccagc ccttctcgga cttcagcatc acgtcggcac tgtagccata
      721 gcacgtgcag ctggcatcca gtacgcgttg gacgtacacg tccacccgat tcaggtacac
      781 gaagtggtag acgtctcgca tgcgcttgtc gccggtgata tcagacagct tggctgccac
      841 tccggcaaag gtgtcgccgt cgttccgtgt gtcgtacaac ggccgaatcc cgcccttcca
      901 gatttgaatc cacggattcg acaccgtggc ggtcatttcc ggataggtga attccatcca
      961 gctgttgcat gcaaacgcca cgtccgcatg gttcacgtct gaggtcatct cgatgtcttg
     1021 cgtgacgatc atctcgatgt tcggatcgac gttcttcacc atgtcatagt ggtgcttgga
     1081 gttattcagc acgtttacgt tcgtgaccca ccgaaccttt gtggggctcg gcatgtgggt
     1141 cttcccggtg aacaccttgc ggccgtactt cggcgtgttg acgatgaggg ccgtgtcccc
     1201 gtggttccag taggccacct cctcgccgta gtagtacgac ttcgtcttga tttccttccc
     1261 gtgcgcattc ggatcgaggg tcagattgaa cggatcctca cccgtgtgca ccgcgatacc
     1321 ggcgccggac cacggcgtcg agttccagat gcccgcctta tagttgccgg cccaggtatg
     1381 ttgcccggtt ccgaatttgc ccacgttgcc tgtgacgatg agcaccatcg ccgcggcgcg
     1441 cgagttttcc gtcatgtgga aatagtgatt ggtcccctcg ccgttatgga tcgctgcggg
     1501 tttgatggtt ccgctatcgc gcgcccaccg gacgaggaga tccttgggac agcgggtaat
     1561 ctgatgggac gtgtccaggt catagtcctg gaaatgaatc agataccctt gccagatcgg
     1621 catcacgtcc gtttcacgtc cgttcagcaa tttgacgcga tacgtccccg tcatcgcggg
     1681 atcgatcccg ctgctctgca tatgccagcc cacctgctca cgatgcaggg ggaccgcctg
     1741 cttcttgttc acgtcccaga ccatcatgcc gccgagccgc tcgatctgct ccggcttcaa
     1801 cgactgaatc cgacccgagt agctcttgga gaagtccggg aacttgtagt ctgcgatgac
     1861 gtcccgcgga tccagatact gcaacgtatc ggtccgcatc agcagcgggg catcagtgaa
     1921 ctgtttgaga aaatccatat cgtgcatgtt ttcgtcgacg atgatcttta gagctccaag
     1981 gaagatcgca cagtccgcgt ttggtctgac ggggatccag tagtcggctc gataggcggt
     2041 gggattgtat tccggcgtga tcacgacgac gcgagcgccg cgctcgatac attccaactt
     2101 ccagtgcgct tccggcatct tgttctcgac gaagttcttg ccccagctgg tgttcagctt
     2161 ggagaagcgc atgtccgaga gatcgatgtc ggatgcctgt gcgccgcacc accaggggtt
     2221 tgagggattc tggtcgccgt gccaggtgta gttcgaccaa taccgtcccc cttgggcctg
     2281 atccggaccg tttttccgaa tccacgagtc caacagcgca ttgacgccgc cattgaaccg
     2341 ggtgttgaac atcttgccga tgatgccgag gaccggcatg cccgcgcgat gcttgaagca
     2401 gcggaccccc gcccccttca tcatctcgat catctctggg gcatagccct gctcccgcag
     2461 ccgtcgggcc cctgcctcac cgctgtagcg ctgcgcgatc acgatggcgc ctttggccac
     2521 ataggtgaag gcggtgtccc aggaggcgcg gaccatgtcg tccaggaagc ggctgtcaaa
     2581 tttgtacttc cgcttcacat cgggcgtgag ctccggggag cccgcatcca tccactcctt
     2641 ccagcccttg
//
LOCUS       DATDNW010000183         4902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_706505, whole genome shotgun sequence.
ACCESSION   DATDNW010000183 DATDNW010000000
VERSION     DATDNW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706505"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VI359_04515"
     CDS             complement(<1..226)
                     /locus_tag="VI359_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973547.1"
                     /translation="MHHAVDQVHHSQTVQRETPFEYHSQDPDIAYSLFMHHSSGIALL
                     AVGLLMLLDRLWLCPRDFTGVLIGCTWLLFG"
     gene            complement(529..1908)
                     /locus_tag="VI359_04520"
     CDS             complement(529..1908)
                     /locus_tag="VI359_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973548.1"
                     /translation="MAMILVVDDENNYLWMLKELLQGEGHEVVTCNKVADALDILQES
                     RIDVLLTDLRMSETDGMTLLAQAREISPTTTTMLMTAYGTIERAVEAMRLGAYDFIVK
                     PFENADLLRSVAKAVEHNTLVRENVRLSHSLAPHYHFDNLIGQSVAMRAVFDKIKRVT
                     DSKTTVLLFGESGSGKELVARAIHFNGPRCGRPFLAVNCGAMTATLAESELFGHERGA
                     FTGANARHQGMFEQANSGTLFLDEIGELPMDLQSKLLRVLDTQEVRRVGGEKTFRVDV
                     RILAATNRDLKAEVKHGRFREDLFFRLSVVRIDVPPLRERGEDVRLLAEAYLRELVKE
                     GSLHGKRFAPATLDLLQRYQWPGNVRELHNAVAHAALMAQHEEIQPDDFPLELAASGE
                     WLHALDRILPPDAPLDPTLKAVEHHMIARALTRAGGVQAKAADLLKISRSLLQYKLKT
                     FGTNPDQAN"
     gene            complement(1929..3284)
                     /locus_tag="VI359_04525"
     CDS             complement(1929..3284)
                     /locus_tag="VI359_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973549.1"
                     /translation="MAFGYLNAFDILKAATLTLLAILLFSLGSVHRPTSFGAYLSFSA
                     IMLLQGIEMLAPSFSEKTNNPAQRLLLLRLSILLQLLLASILVAVTDGSGSIYELVYL
                     LPIISAATKLPGRDVAIVVGGSVLAMIGFIVTGEQLTASIMSVKEFQDSVAALVYFTM
                     AGLLTYFFAKSEREQRLRYQTLAATLANTNEELRETQTQLTDRLAELAMMDERVQRVS
                     QMAALGELAGQLAHEVRNPLGIIRGAAEMLAGRVSDPSTYRHITVLIEEADRLNKAVE
                     GVLRLSMPLRMKLLRLDLVELLEGIAQISSASSVPIGSSVKLIVPSEPLWIRGDRDLL
                     YQAFTNLVRNAFQAMPTGGIVTITVDSTSDDGHVVTSIADAGIGLSEEDVRRLGEPFF
                     SKRPTGVGLGFSLARRVVSEHGGSVTVGSILGQGTTVSIHLPILKAIREKQLPVESFN
                     A"
     gene            complement(3406..4101)
                     /locus_tag="VI359_04530"
     CDS             complement(3406..4101)
                     /locus_tag="VI359_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973550.1"
                     /translation="MAALFLLGISTAFVQAEQQVLSSDRTSALERELHTLLANQAPAS
                     SDPRILVHLADLYLDLGDDAYSEETQRRAAYEEGARLARRAVELDESNAEAHYLYAAN
                     LGSAAQLKGVMASAFTVHDLKRHVRRALELNPNHAPALHMMGMMLEELPWVLGGDAEA
                     ALTYLNKAVTTDPHYIHARLDLGKAYIKRQQIDAARHELKTILSQAPPREQSASEQRY
                     RQEARQLLASLAP"
     gene            complement(4287..>4902)
                     /locus_tag="VI359_04535"
     CDS             complement(4287..>4902)
                     /locus_tag="VI359_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEY6973551.1"
                     /translation="WAYRDRILDLRKDLRFRYILIFKNHGAAAGATLEHSHSQLIALP
                     IIPTSVMTEIEGCRAHYEQKERCIYCDILRQDLSEGDRIVAENPEFACVTPFAPRFPF
                     EMWILPKRHAGYFQECQKQQFEFLAPILSESLRRMDKVLAKPAYNFVLHSSPLHEKTG
                     DFYHWHLEIIPKLTQVAGFEWGTGFYINPVSPEESAKFLREAEI"
BASE COUNT         1097 a         1300 c         1404 g         1101 t
ORIGIN      
        1 tgccgaacag cagccacgtg catccgatca gtacccctgt aaagtcacga ggacagagcc
       61 acagccgatc gagcagcatg agcagcccta cggccaacag agcaatacca gagctgtgat
      121 gcatgaacag agaataggca atatccggat cttgtgagtg atattcgaac ggggtttccc
      181 gctggactgt ctgagaatgg tgaacctggt cgactgcgtg atgcatatca gagtctgagg
      241 cgcttccctc agatggaaag aggagcaacc cggaaaaaag tccgacgatg agaaggattg
      301 ctgcggctag cctcacgcta ctcactcttg atccgcgcct ggtccggcca aactgacaag
      361 cgaatcaata gataaagagc acgaatctcg ccacagtggt gtcagtgagg actctattgg
      421 aatttcaaga agttagacga acgacagaga tggcggaatg cgaggggcct ggcagcgtgt
      481 caaaatattt tgtttgccgt caaaattctt atacaacgtc agaagacttt aattggcttg
      541 atcaggatta gtgccaaacg tctttaactt atactgaagc agactccggc tgatcttgag
      601 taaatcggcg gccttggctt gaacacctcc ggcacgagtc agagcacggg cgatcatgtg
      661 gtgttcaacc gctttgagcg tcgggtcgag tggggcatcg ggaggaagaa ttcgatcaag
      721 ggcgtgcaac cattcgccgc tggcggctaa ttccagcgga aagtcgtccg gctgaatttc
      781 ttcgtgttgg gccatcaatg ccgcgtgtgc gacggcgttg tgcagctcac gcacgttccc
      841 cggccattgg taacgctgca ggaggtcgag agtggctggt gcaaaccgtt ttccgtgcaa
      901 acttccctcc ttaaccagtt ctcgcaaata cgcttctgcc aataaccgaa catcttcgcc
      961 tcgttcgcgt agaggaggga catcgattcg cacgacgctc aggcggaaaa agaggtcttc
     1021 tcggaagcgg ccgtgtttta cctccgcttt caagtctcga ttggtggcgg cgagaattct
     1081 cacgtcgaca cggaacgtct tttcgcctcc cactcggcgg acctcctggg tgtcgagcac
     1141 cctcaggagc tttgactgga gatccatcgg aagttctcca atctcatcca gaaagagagt
     1201 tccagaattc gcctgttcga acatcccctg atgtcttgcg ttggcaccgg tgaatgcgcc
     1261 tcgttcgtgt ccaaagagtt cgctttccgc aagtgtggct gtcatggccc cgcagttgac
     1321 tgccaggaac ggccggccac agcgcggccc attgaaatga atggcacgcg caaccagttc
     1381 cttcccactt ccgctctcgc caaagagtaa aacagtggtc tttgagtcgg tgactcgctt
     1441 gatcttgtcg aagacggctc gcatggcgac actctgtcca atcaggttat cgaagtgata
     1501 gtgaggcgcc agggagtgag acagacgcac attctcccga accaaagtgt tgtgttcgac
     1561 cgcttttgct acggaacgga gtaagtctgc gttctcgaat ggcttcacaa taaagtcgta
     1621 cgccccaagc cgcatcgctt ccaccgcccg ttcgatcgtc ccgtaggccg tcatcagcat
     1681 cgtggtggtg gttggtgaaa tctctcgtgc ttgggccagc agtgtcatgc catccgtctc
     1741 gctcattcgg aggtctgtga gcagcacgtc gattcttgac tcctgaagaa tgtcgagggc
     1801 atcggctacc ttgttgcatg tgacgacctc atgcccttct ccctgaagaa gctccttcag
     1861 catccagagg taattgttct cgtcatcaac gaccaggatc atggccatag gcggatttcc
     1921 gattctttct aagcgttgaa agactccact ggcaattgtt tctcgcggat ggcttttaga
     1981 atcggcagat gaatggacac tgtcgtccct tgtcccagga tactccccac agttaccgat
     2041 cctccgtgct ctgacacgac ccgtcgagca agtgaaaatc caaggccgac tccagttggt
     2101 cgtttggaaa aaaacggttc gccgagtcgt ctgacgtctt cctcggagag gccgataccg
     2161 gcgtcagcga ttgaggtcac gacatgacca tcatcggatg tggagtcaac cgtgatggtg
     2221 acgataccac cggtcggcat agcctggaat gcgttgcgga cgagattggt aaaggcctga
     2281 tacaggagat cgcggtctcc gcgaatccac agtggctctg acggaacgat gagcttgacg
     2341 gaagaaccaa tgggtacaga cgaggctgag gaaatctgtg cgattccctc gagcagttcg
     2401 acaaggtcta ggcgcagaag tttcattctc agaggcatgc taagacggag gacgccctcc
     2461 accgccttat tgagtcgatc cgcctcctcg atcaagaccg tgatgtgtcg gtaggtagag
     2521 ggatccgaca cacggccggc cagcatctcg gccgcacccc tgataatccc gagtgggttc
     2581 cgaacctcat gagccaattg gccggcgagt tcaccgaggg cagccatttg gctaactcgc
     2641 tggacacgtt catccatcat ggccagttcg gccagacggt cggtcaattg tgtttgcgtc
     2701 tcgcgaagct cctcgttggt gttggcgagc gtggccgcga gggtttgata gcgcagccgc
     2761 tgctctcgtt cactcttcgc aaaaaagtag gtcagcaagc cggccattgt gaaatagacc
     2821 aaggctgcga cggaatcctg gaactccttg acgctcataa ttgatgcggt cagttgttct
     2881 ccggtcacaa tgaacccgat cattgccaac accgagccgc ccacgacgat cgcgacgtct
     2941 cgacccggta acttggtggc tgctgagatg atcggcagca aatacacgag ttcatagatg
     3001 ctcccgcttc cgtccgttac tgccacgagg atgctcgcca acaagagctg aagcagaatg
     3061 ctcaggcgta acaggagaag acgttgtgca gggttatttg tcttttcgga aaaggacggc
     3121 gccaacatct cgatgccctg caacaacatg atggcggaaa acgaaaggta agcaccgaac
     3181 gacgtcggac ggtgcacgct gccgagggag aacaagagga tggcgagcag agttaaggtc
     3241 gcagccttca gaatgtcgaa cgcgttgaga taaccgaaag ccatggagag tcgatctttt
     3301 caggtgatca gcaaattcga agaactctac aggctgctgt gaggagaagt caaaagtcga
     3361 gactcgtgtc tcaacgcatt atttgtgtga gtcagttgtg ttttgtcaag gtgcgagaga
     3421 cgcgagaagc tggcgggctt cctggcggta acgctgctca ctcgctgact gttcgcgagg
     3481 gggggcctgg ctcagaattg tcttcaactc gtgccgtgct gcgtcgattt gctgtcgctt
     3541 gatgtaggct ttcccgagat caagacgtgc gtggatataa tgtggatcgg ttgttactgc
     3601 cttattcaga tatgtgagcg ccgcctccgc atcaccccca agcacccatg gcaactcttc
     3661 gagcatcatc cccatcatgt gaagagccgg ggcgtggttg gggttgagtt ccagcgcgcg
     3721 gcggacatgt ctcttgaggt cgtgaacggt gaatgcggat gccatcactc ccttaagctg
     3781 ggcggcactg ccgaggttgg cggcgtataa gtaatgagcc tctgcgttgg actcatcgag
     3841 ctcaacggct cggcgggcga gtcttgctcc ttcctcgtag gcggctcggc gttgtgtttc
     3901 ttcggagtag gcgtcatctc cgagatcaag atacaagtcc gcgagatgca cgaggatccg
     3961 cgggtccgac gatgcaggcg cctggttggc aaggagcgtg tgcagttctc gttcaagagc
     4021 gcttgtgcga tcggaagaca gtacttgttg ttcagcctgc acgaatgccg ttgatattcc
     4081 caggaggaat agagctgcca cgagcagtct ccttcctttg agactcatct ccatcctaat
     4141 ttctttaagg gaagaggaaa gtcgaaatca agagacatcc gtgatgcgcc ccacgccgtg
     4201 gcttgccgac ttcgaacata atcatcgtga acgtcagcct gttgtctcag ccgctgtgat
     4261 gcagtttcag ggaggatggg aaacagtcag atctctgctt cccgtaggaa tttagctgat
     4321 tcttccggcg acaccgggtt gatatagaac cccgtgcccc attcgaagcc ggcgacctgc
     4381 gtcagcttcg gaatgatttc aaggtgccag tggtagaagt cgccggtctt ttcgtggagc
     4441 ggagaactat gaaggacgaa attgtaggcg ggcttggcga ggactttgtc catccgccgc
     4501 aacgattcgg acagaatcgg cgccaagaac tcgaattgtt gtttctggca ttcctggaag
     4561 taacccgcat gccgcttcgg cagaatccac atttcgaacg gaaaccgcgg ggcgaagggc
     4621 gtcacacaag caaactcggg attctcggcg acgatccggt cgccttccga taagtcctgc
     4681 cgcagaatgt cgcaatagat gcaccgttct ttctgctcat aatgcgcgcg gcacccttcg
     4741 atttccgtca tgacgcttgt agggatgatc ggcagggcga tgagctgcga gtggctatgc
     4801 tcgagcgtcg cgccggccgc ggcgccatga ttcttgaaga tcaggatgta gcggaagcga
     4861 agatcctttc ggaggtccag tatccgatcc cgataggccc ag
//
LOCUS       DATDNW010000184         4262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_443678, whole genome shotgun sequence.
ACCESSION   DATDNW010000184 DATDNW010000000
VERSION     DATDNW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443678"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VI359_04540"
     CDS             <1..754
                     /locus_tag="VI359_04540"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY6973552.1"
                     /translation="GAPDAIFHAAGGSSVAQSVTHPIRDFDRTVRTTSVLLDTIRRAA
                     PEALLMLPSSAAVYGIADNGPIKETASLNPVSPYGLHKCLAEQLIQGAHRLFGLRYAI
                     IRYFSIYGPGLRKQLLWDLAQKLSSKPKDIVLFGTGEETRDFLHIEDAARFAVLVAEK
                     TAGREPLIVNGGSGECVSVRHVAKTLGGLLSPETDIRFNGTQRTGDPFHYQADTCLMH
                     HLGFRPMWNLKKGMAGYVDWLVHSDGDYRKCA"
     gene            802..1941
                     /locus_tag="VI359_04545"
     CDS             802..1941
                     /locus_tag="VI359_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY6973553.1"
                     /translation="MRNLLLALNQVPSAEPVFLVPASKPDSDLAPWIATGCEVLRIQA
                     LDRWTPSWWLRQFARFTRRVEHDVWSESFLRHHGIEVTYLRPCVGRPGAIPNISWVPD
                     FQHLHLPDMFSGEEIETRSAQIRLAAEYSARIVLSSHQAHQDYRRFSPAHAHKGRVLQ
                     FVASVSPEVYRRNPNEIVQKYGVPEKFFYLPNQFWKHKNHLVVVKALGLLKKMGRRVH
                     VVCTGNPKDYRHPRHWEDFHRAISENGVEGQLIVLGMIPADDVFALLRQSIATVNPSL
                     FEGWSTTVEEARSVGKRVLLSDISVHLEQDPPGATYFDRNNPDDLAQKLYELWQEVSP
                     GPDVVMETRARESLPARLAEFGARFVAIAQEASAGMTRRGVWGAA"
     gene            1958..2803
                     /locus_tag="VI359_04550"
     CDS             1958..2803
                     /locus_tag="VI359_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973554.1"
                     /translation="MQVKLDERPAEVCNYNELHARRAADLINLKGASVLVVGANTGAD
                     CRYFIGWGAKEVHGLDVVPNVGCEYQADNVRYFCMSAEAMQLPDNFYDLVYCFATMEH
                     VPDIVAAFREMTRVTKPGGFIYSVASPLWNSRSGHHMACLAPHPWIHLRMGKQEMVRY
                     CKSDGIEGERGLSIESIVSYVLDSTEFNRRPARDYLQAVATIPNVLIVSNLLYNEPES
                     AVPEDLRTELAQKGISHDELRAVTHEFVAIKRPLTWKQRIVGTTRRLVRVARTQFSGI
                     RRLLK"
     gene            2997..4133
                     /locus_tag="VI359_04555"
     CDS             2997..4133
                     /locus_tag="VI359_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6973555.1"
                     /translation="MRPLKLVLCVVGLGTGGTETHVLELASRINQARFDLTVCSLKSG
                     GCMADELRARGIRVVSLGGTGKLDARVMLRLWRFVRREQPDVIQAFLFWANVASRLVG
                     HFEKTIQVISSYHDEVVPEGWLNRAIDRLTMKWTKYIVCCSESVRRSVGRRIGGAKEQ
                     FVVIPFGVETSRFGDVGPMAGTVLGLQGGLPIIGTVCRLVEPKKGLRYLLEAMAHLEQ
                     EAGKPVCQLVIVGEGPAEKTLRALSAQLGIASRVVFAGMRRDIPQLLAMTDIFVLPSL
                     YEGFGIAILEAMATGKPVVASTVGGIPEFVVSGESGLLVPPGDSIALAAAIRQLLAQP
                     EWAKLMGRRGQEHVRQHYSIESVVRQHEQLYEMCVAQTSTLAHA"
BASE COUNT          916 a         1093 c         1311 g          942 t
ORIGIN      
        1 cggggcacca gacgccattt ttcacgccgc tggcggaagt tccgtcgcac aatccgtgac
       61 ccatccgata cgcgactttg acaggacggt gagaaccacg tcggtgctgc tggataccat
      121 tcgtcgggcg gctccggagg ctctgctgat gttgccctcc agcgcggccg tttacggaat
      181 agcggacaac ggcccgatta aggaaacggc ctcgctgaat ccggtgtcgc cctacggtct
      241 ccacaagtgc ctagccgagc aacttatcca gggtgcacac cgactcttcg gtctccgtta
      301 cgccatcatc cggtactttt ctatttacgg cccgggctta cgcaagcaat tgctgtggga
      361 tctggctcag aaattatcat caaagccgaa agacatcgtg ctgtttggga cgggtgagga
      421 gacaagagat ttcctgcata ttgaagatgc cgcgcgcttc gcagtcttgg tggcggaaaa
      481 gacggcagga cgggagccat tgatcgtaaa cggtgggagt ggtgagtgcg tttccgtccg
      541 acacgtcgca aaaacccttg ggggcttgct cagtccggag acggacattc gattcaatgg
      601 tacccaacga actggagatc cgtttcacta tcaggcggat acatgtctga tgcatcacct
      661 cggttttcgt cccatgtgga atttgaagaa gggaatggcg ggatacgtgg attggctcgt
      721 gcattctgat ggtgactaca gaaagtgcgc ataggaattt ttcacactgg tggatccgag
      781 tggacgggcg gagaacgcta tgtgcgcaat ctgcttctgg cgctcaacca agtcccgtcg
      841 gcagaaccgg tctttcttgt gcccgcgagt aagcccgatt ccgacttggc tccctggatc
      901 gcgacgggat gcgaggtctt gcggatacaa gcgttggacc ggtggacgcc gagttggtgg
      961 ctcaggcagt ttgcccgatt caccagacgg gtggagcatg acgtatggtc cgagtcgttt
     1021 ctcagacatc acggcatcga ggtcacgtat ctgcgcccct gcgtcggccg gcctggcgca
     1081 atcccaaata tttcatgggt tcccgatttc caacatctcc atctcccgga tatgtttagt
     1141 ggagaggaga ttgaaactcg gagcgcgcag atacgtctgg cggctgagta ctctgcgcga
     1201 atcgtgttga gtagccacca agcccaccaa gattaccggc gattctctcc ggcacatgcg
     1261 cacaaagggc gcgtgctgca atttgtcgcg tcggtcagtc ctgaggtcta ccgacgtaat
     1321 cccaatgaaa tcgtacagaa atatggagtg cccgaaaagt tcttctactt gccgaatcaa
     1381 ttttggaaac ataagaacca tctggtcgtg gtgaaggcct tggggctgct caagaaaatg
     1441 ggccgccgtg tgcatgtcgt atgtaccggt aaccccaagg attaccgcca tcctcggcat
     1501 tgggaggact ttcatcgagc gatcagtgag aacggggtcg aaggtcagct gatcgtgctc
     1561 ggcatgatac cggccgacga cgtctttgct ctgctgagac aaagcattgc gaccgtcaac
     1621 ccgtctttgt ttgaagggtg gagcaccacg gttgaagaag ccaggtctgt cggcaagcga
     1681 gtgttgttgt cggatatttc cgttcatctg gagcaagacc cgccgggcgc gacctatttc
     1741 gatcgcaaca atccggacga tctcgctcaa aaactgtatg aactgtggca agaggtttct
     1801 cccggaccgg acgtcgtgat ggaaacgaga gcacgggaaa gtctgcctgc ccgcctggca
     1861 gaatttggcg cgcgattcgt cgccattgca caagaggcat ccgccggcat gacgcgtcgc
     1921 ggagtttggg gagcggccta ggacgccaag gaggaacgtg caagtcaagc tggacgagag
     1981 acctgccgag gtctgtaatt acaatgaact ccacgctcgc cgggcggcgg atctcatcaa
     2041 cctaaagggt gcgagcgtgt tggtggtcgg tgccaatacc ggtgcggact gccgctattt
     2101 tattggctgg ggcgcgaagg aagtccacgg acttgatgta gtgccgaatg tcgggtgcga
     2161 gtatcaggca gacaacgtgc gttacttctg tatgtctgcc gaagcgatgc agttacccga
     2221 caatttctat gatctggtgt actgctttgc cacgatggaa catgtacccg acatcgtagc
     2281 ggcgttccgt gaaatgacga gagtgaccaa gccaggcgga ttcatctatt cggtcgcgtc
     2341 gccgttgtgg aactcgagaa gcggtcatca catggcctgc ctcgctccac atccttggat
     2401 ccatctccgc atgggcaagc aggagatggt tcgttattgc aaaagcgacg gcatcgaggg
     2461 cgaacgaggg ctttcaatcg agtccattgt atcctatgtg ctggattcaa cggagtttaa
     2521 tcgtcgacct gctcgtgact atctgcaagc agtggccacc attccgaatg tgctgatcgt
     2581 cagcaacctg ctctacaacg agccggaatc tgctgtcccg gaagacctga gaactgagct
     2641 cgcgcagaaa ggcatttcgc acgatgaact ccgagccgtg acccatgagt tcgtggccat
     2701 caaacgcccg ctgacgtgga aacaacgaat cgtgggaact accagacgtc tggtgcgtgt
     2761 ggctcgcacg caattcagcg gaatacgtag acttttgaag tgaagtcggt accatggaaa
     2821 gggatcacaa tcgtggatcc cgtcgggccc caataatcag gaccgttctg cgggttgaag
     2881 tctctttcga ctgatttggc aaggtcgcgc caggtgtgga ggggacggtc aagaggaggg
     2941 gtacatgaag gaataggaat gcccgtccgt caccgcatgg ccaacagaac ccaattatga
     3001 gaccgctgaa gcttgttttg tgtgtggtgg gactcgggac ggggggaacg gaaacgcatg
     3061 tgctcgaact cgcctcccgc atcaatcagg cgagatttga cctgaccgtc tgctcgctca
     3121 agtccggcgg gtgtatggca gacgaattgc gggcgcgcgg aattcgcgtg gtgtctctcg
     3181 gtgggacagg gaaattggat gctcgagtga tgctacgact ttggcgattc gttcgacgtg
     3241 aacagccgga tgtcattcaa gccttcctct tctgggccaa tgtcgcatcg aggctggtgg
     3301 ggcatttcga gaaaaccatt caggtcatct cgtcgtatca cgatgaagtc gtgccggagg
     3361 gctggctcaa ccgtgcgatc gatcgtctga ccatgaagtg gacgaagtac atcgtgtgtt
     3421 gttccgagtc cgtgcgccgg tcggtggggc gacgaattgg tggagcgaag gaacaattcg
     3481 tcgtcattcc gtttggagta gaaacgagtc gatttgggga tgtgggtccc atggcgggga
     3541 cggtattggg attgcaggga ggattaccga tcatcggaac cgtctgtcgc ttggtcgagc
     3601 ccaagaaggg gcttaggtat ctgctggagg cgatggcaca tttggaacag gaggccggga
     3661 aaccggtctg ccagctggtg atcgtcgggg aaggcccggc ggagaagacg ctccgggcct
     3721 taagtgcaca gctgggcatc gcttctcggg tggtctttgc aggaatgcga cgagatatcc
     3781 cacaacttct tgcaatgacg gatatcttcg tcctgccgtc gctgtacgag ggattcggga
     3841 tcgccattct tgaagcgatg gcaacgggga aaccggtggt ggcctcaacg gtgggaggga
     3901 tcccggaatt tgtcgtttcg ggagaatccg gcctcttagt gccaccggga gattcgatcg
     3961 cattggcggc tgccatccga caattgctcg cccagccaga gtgggccaag ctcatgggac
     4021 gccgtggaca ggagcatgtg agacagcact actccatcga gtcggttgtg agacagcacg
     4081 aacaactcta cgagatgtgt gtcgctcaga cttcaacttt ggcacatgcg tgagagcatg
     4141 cacctttatt ttctgacctt cattctgggc ctgttcttct cgctctacgg agtgccgatt
     4201 gcgagggagg cggccctcaa atacggcatc gtcgacgcgc ccgatgggca gttgaagcat
     4261 ca
//
LOCUS       DATDNW010000185         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1115056, whole genome shotgun sequence.
ACCESSION   DATDNW010000185 DATDNW010000000
VERSION     DATDNW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115056"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1745)
                     /locus_tag="VI359_04560"
     CDS             complement(96..1745)
                     /locus_tag="VI359_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY6973556.1"
                     /translation="MKIVVGLGVIVLLLIGLILSLPFLIDLNKYQDQYKPLIEDALNR
                     KIQLQDIRLTIWPRIGARVAGFAVLDDPAFEAGSFASLTSLDVGVKLMPLLGGKVEVE
                     EIALRDPVITVIKNKNGVLNVSTLGRTGVAAPATPSRAPVPSAEGPLKILALLAVDQV
                     SISGGKLIYRDLSTTNSTEYILQDLELLLKSVRLGQTPSLHLATLVQPFNMPVALDGT
                     FGPLKETTDIDAINMQLALGKTSFTITGSTVGHDAKINIGSPVINTANLPVALPLKKP
                     VEIHDLQVVAEVKGQEAQLHSLSLHLFDGEVKTQGKLISGSDAPPFSGKLTMEGLQLG
                     PALDAVAAPQVSVSGSGRADMNLQGRGFSMADLTKSLEGTGHVAVKEGKVEGVSLLQE
                     AISILKVAGISLDNPKATAFSTIETDLAIKQGIIYVQRLLMDSHDFQATGGGTIGFDQ
                     VLNLTVNLNLSQDLSHKIAAASPVAKLAIRDGRLVLPLLITGTAQAPSYGLDMKGISG
                     KVQEQVQKKVEEAVGGLLKGTTKPEDLKQQGQELFKDFLGR"
     gene            1899..1988
                     /locus_tag="VI359_04565"
     tRNA            1899..1988
                     /locus_tag="VI359_04565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1933..1935,aa:Ser,seq:gga)
     gene            2086..2185
                     /gene="ffs"
                     /locus_tag="VI359_04570"
     ncRNA           2086..2185
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VI359_04570"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2293..>3418
                     /gene="dnaX"
                     /locus_tag="VI359_04575"
     CDS             2293..>3418
                     /gene="dnaX"
                     /locus_tag="VI359_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY6973557.1"
                     /translation="MDYQVSARKYRPGTFNDVIGQPHVVQTLVNSITTKRIAHAYLFS
                     GTRGVGKTTVARILAKALNCERGPTGTPCGTCANCQEIAQGTSVDVIEIDGASNTSVD
                     DVREIRENVKFAPFRGQYRVYIIDEVHMLSNSAFNALLKTLEEPPSHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIARTEIVERLRHVAQQDGIALEDRSLTALARASEGSMRDAL
                     SLLDQAVAFGGTTIVHADLEALLGAVPQELVRGMIQAITGQDSAAALRMLANLLDQGH
                     DLRAYCADVVEYLRNMLVASVVTAPQDLRGLIEASAEDLLQMAADARLLSPEQLQELF
                     AIFTQAEDALRFSAHPRFILETAAVRATRLARRSDGKPDQG"
BASE COUNT          713 a          978 c          977 g          750 t
ORIGIN      
        1 ccggcggtga aaatcccaag cgccacaacg gcttgcaggc cgtactgcac tgcgtattgc
       61 gtgacggtat ccatggcaat catgtggcga tcctcctagc ggccaaggaa gtccttgaac
      121 aactcttggc cttgttgctt cagatcttcc ggtttcgtcg tgcctttcag aagcccaccg
      181 acagcttcct caactttctt ctgcacttgt tcttgaactt tcccactaat ccctttcata
      241 tccaatccgt atgagggggc ttgcgcggtg cccgtgatga ggagcggcag aaccagtcgc
      301 ccgtccctga tggccagttt ggcgacgggc gaggccgcgg cgatcttgtg actgaggtct
      361 tgtgagaggt tgaggtttac ggtgagattc agaacttgat caaatccgat ggtgccgcct
      421 ccggtggctt ggaaatcatg gctatccatc aagagtcgct gaacatagat gattccttgc
      481 ttgatggcga gatcggtctc gatggtcgag aaggctgttg ctttcggatt gtccagcgag
      541 ataccggcga ccttgaggat ggagatggcc tcctgcaaca gactgacacc ttcgactttg
      601 ccctctttca ccgcgacatg gcctgttcct tctagagatt tggtgagatc cgccatcgag
      661 aaaccccgac cctgcaagtt catatcggct cgacccgatc cgcttaccga cacttgcggg
      721 gcggcaactg cgtcgagggc cggaccgagc tgcaagcctt ccatcgtcag tttgccacta
      781 aatgggggag catcggaacc ggaaataagc ttcccctgcg tcttcacctc tccgtcgaag
      841 aggtgcaacg acaaggagtg aagctgcgcc tcttggcctt tgacctctgc gacgacctgg
      901 agatcgtgaa tctcaaccgg tttctttaac ggtagggcga ctggcagatt ggccgtgtta
      961 atcaccggtg agccgatatt gatctttgcg tcgtgtccga ccgtgctccc ggtaatcgta
     1021 aaggacgttt tcccaagtgc gagttgcata ttgatggcgt cgatgtccgt cgtttctttc
     1081 aggggtccga atgtgccatc cagtgccacc ggcatgttga acggttgaac gagtgtcgca
     1141 aggtggaggc tcggggtttg acccagccgg acggatttga ggagcagttc caagtcttgc
     1201 aatatgtact ccgtcgagtt ggtcgtcgac agatcgcgat agatcagttt cccgcccgag
     1261 atggagacct gatcaaccgc cagcagtgct agaatcttga gcggaccttc ggccgatgga
     1321 acaggtgcac gcgacggcgt ggcaggagcg gccacgcccg tgcgaccgag cgtcgacaca
     1381 ttcaagacgc cgttcttatt cttgatgacg gtgatcaccg ggtctcgcag cgcgatctct
     1441 tccacttcga ctttgccgcc caacaggggc atcagcttga cgcctacatc aagcgacgtg
     1501 agcgatgcga atgaacctgc ttcaaacgcc ggatcgtcca acacggcaaa tccggccacg
     1561 cgcgcaccga tgcgcggcca gatagtcagg cggatgtctt gtagttgaat cttgcgattg
     1621 agcgcgtctt cgatgagcgg cttgtactgg tcttgatact tgttcaggtc gatgagaaac
     1681 ggcaaagaga ggattaatcc aatcaggagt aagacgatca ccccgagccc gaccacgatt
     1741 ttcattgagc gcctccattc acttgaccgc agtataggaa agggatttcc ggcggtcaat
     1801 gtctggcgag cagacctgcg tcgactggtg cgtctggttg tcttgctggt gctcagagcg
     1861 cgtgctaaga tgctcgccgc ctggcatgag gtcggtgtgg agaggtgcga gagtggacga
     1921 atcgacatgc ttggaaagca tgcgtctcgg caacgggacc gtgggttcga atcccaccct
     1981 ctccgccaaa cccaagcgct gagtagtgag tgctgagcaa tgaggtgtca gaatcaagcg
     2041 gatcctgttt ccagtccgca ctcagtcctc agcacttcga aaaggggctg ggccctgtgc
     2101 aacagaaccc tgtgaacccc gccaggtccg gaaggaagca acggtaagca gttagtcctg
     2161 ggtgccgcag gatcacctgg ccccgctctg gaacacgtga ggcgtgaaac gtgagacgta
     2221 aggcgagatc cgcttccggc tatcacgctt cattcgcctc acgccttacc ccttacgcct
     2281 aacgcgcttt ttatggatta ccaagtctcc gcccgcaagt atcgccccgg gacgtttaac
     2341 gacgtgatcg gtcagcccca tgtcgtgcag acgttagtca attccattac gaccaaacgg
     2401 attgcacatg cgtacctgtt ctccggcact cgcggggtgg ggaagacgac cgtggcgaga
     2461 attctcgcaa aagcactcaa ttgcgagcgg ggaccgaccg gtacaccctg cgggacttgc
     2521 gcgaactgcc aagaaatcgc tcaaggaacg tcggtcgatg tgatcgaaat cgacggcgca
     2581 tccaatacga gcgtggacga cgtgcgcgag attcgcgaga atgtgaagtt cgcgcccttc
     2641 cgcggtcagt atcgtgtcta catcatcgac gaagttcata tgttgtccaa ttcggccttt
     2701 aacgccctcc tcaagacgtt ggaggaacca ccgtcgcacg tcgtcttcat tttcgccacc
     2761 accgaaatcc ataaaattcc agccacgatc ctttctcggt gtcagcacta taatttccgt
     2821 cgcatcgcgc gcaccgaaat tgtcgagcgg ttgcgccatg tcgcgcagca ggacggcatc
     2881 gcactcgagg atcgcagcct aacggcactc gctcgggcca gcgaggggag catgcgtgac
     2941 gcgctcagct tgctggatca agccgtcgca ttcggcggaa ccacgatcgt tcatgccgac
     3001 ctcgaggcac ttttaggggc tgtgcctcaa gaactcgtcc gcgggatgat ccaggcgatc
     3061 accggtcaag acagcgcagc agcccttcgc atgctggcca atctgctcga tcaagggcac
     3121 gatctccgag cctattgcgc cgacgtggtt gaatatctgc gtaacatgct ggtggcgtcg
     3181 gtcgtcacgg cgccccagga tctacggggg ctgatcgaag cgtcagcgga agacctgtta
     3241 caaatggcgg ctgatgctcg attgctctcg cccgagcaac tccaggaact cttcgccatt
     3301 tttactcaag cagaagatgc gctccgattc agcgcccatc ctcggttcat ccttgaaacg
     3361 gcggcggttc gtgccactcg cttagcccga cgctcggatg gaaaacctga ccagggga
//
LOCUS       DATDNW010000186         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1115116, whole genome shotgun sequence.
ACCESSION   DATDNW010000186 DATDNW010000000
VERSION     DATDNW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115116"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VI359_04580"
     CDS             complement(<1..318)
                     /locus_tag="VI359_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6973558.1"
                     /translation="MARRIAIIQGHPDAQARHFGHALADEYAKGAEDGGHEVKRIEVA
                     KLDFPILRTKEDFEHGMPPDSIKQAQDTINWADHLVIIYPLWLGSVPALLGAFFEQVF
                     RPGF"
     gene            complement(446..934)
                     /locus_tag="VI359_04585"
     CDS             complement(446..934)
                     /locus_tag="VI359_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973559.1"
                     /translation="MRSSVDNFRALMIPIAVGAIALAGIGVGIPSTVQAEDEMKVEIT
                     ITDKGYDVKGHTTPGALTRIVVRNEGSMTHGITSPMFKEGVLKKEGDGVEIRGPKGKG
                     IRAYHLDAGKTMTLTFKKESRPDPATGISETSQVPFWCDIHTHMKGEFLVVETKGEVG
                     GG"
     gene            complement(1025..1159)
                     /locus_tag="VI359_04590"
     CDS             complement(1025..1159)
                     /locus_tag="VI359_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973560.1"
                     /translation="MKHFANVLALLVNVESTVKTEVADEIRFGRNQRASVLQFPKNNK
                     "
     gene            1546..2034
                     /locus_tag="VI359_04595"
     CDS             1546..2034
                     /locus_tag="VI359_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY6973561.1"
                     /translation="MPILKRLQSYLDNHKIPYEVVSHPTAYTAHDVAETLHVSGNVFA
                     KVVMVKADERFVMAVLPSTWRVDLKRLRDVLEAREVRLATERECADLFPDCDVGTMPP
                     FGNLYGVEVYVDQLLTKDESIVFEAGTYVGAMKLRYKHFAELVRPKVAAFHHEPSKLA
                     PY"
BASE COUNT          429 a          623 c          518 g          499 t
ORIGIN      
        1 aaaaccgggg cgaaagacct gttcgaaaaa cgctcccagg agtgcgggca ctgatccgag
       61 ccagagagga tagatgatta cgagatggtc cgcccagttg atggtatcct gagcttgctt
      121 aatcgaatcc ggcggcatcc cgtgctcgaa atcttctttc gtccgcagga tgggaaagtc
      181 cagcttcgcg acttcaatcc gtttcacttc atggccgcca tcctcagcac ccttcgcata
      241 ctcatcggct aacgcatgac cgaaatggcg cgcctgggca tctggatgac cctggatgat
      301 cgcgatgcgt ctcgccattg ttcactctga ctcggtcagc actctggaac tcagaaacaa
      361 gaacgggccg tgcgactttc ccagatcgca cgacccgttt tgtcccaact actcactccg
      421 actgatctca catcttgatg aagaatcagc caccacccac ttcgcccttc gtctcaacaa
      481 ccaagaattc tcccttcatg tgggtatgga tatcgcacca gaacggaacc tggcttgtct
      541 cgctgattcc ggtcgcagga tctggacggg actccttctt aaaggtcaag gtcatggtct
      601 tccctgcgtc gagatgatag gccctgatcc ctttgccctt cggacccctg atctccactc
      661 cgtcgccctc tttcttgagg actccttcct tgaacattgg cgaggtgatc ccatgagtca
      721 tcgagccctc gttacggacg acgatcctgg tcaacgcgcc gggcgtggta tgacccttca
      781 catcatagcc tttgtccgtg atcgtgatct cgaccttcat ctcatcctcg gcctggacgg
      841 tcgatgggat gccgacaccg attccagcga gagcaatcgc tcccacggct atcggaatca
      901 tcaatgcacg aaaattgtcg accgagcttc tcataacacc ctcctgtggt tgaagtgaac
      961 gatctgtgtt ctcatcaagg tgcattcctc atggcccggc cgccagttgt ttgccaaacc
     1021 ttgatcactt gttatttttg ggaaactgca acaccgatgc ccgctggttt cggccaaaac
     1081 ggatctcatc ggcgacttcc gttttcacgg tgctctcaac attgactaac agggccagca
     1141 cgttagcaaa atgcttcatt gagggctcat acccccttcg atctttaccg ctcgctcgct
     1201 cttttgagcc tgttccaatc tgcgacagca gtcgaagaag gcctgtggag taatgcccct
     1261 gtgttgacgc attgtgcgat tcatgagcaa cccaaacccg cgagcgtgtg attgaactgg
     1321 cgcacagatg catttcagga cagttcgatt catcagcatt gccccattct gctacgagac
     1381 tgatccagca tcttccccgc acttctgtct tgcctgcgct aaccacgctt cctttctcac
     1441 gtttattcgc gacccatcaa tcgtcgcgtg atggcactcc ctttgctcac atcttctttc
     1501 gagccctgat tcgcaaatcg gcatcccttc tttcaggagg tcgacatgcc tattctcaaa
     1561 cggctgcaga gttacctgga caaccacaaa attccctacg aggtggtgag ccacccgacg
     1621 gcctatacgg cgcacgacgt cgccgagacg ctccacgtct cgggtaacgt gtttgccaag
     1681 gtcgtgatgg tgaaagccga tgagcgcttt gtgatggcag tgctcccctc gacatggcgg
     1741 gtcgatctca aacggttgag agacgtgctg gaggctcgcg aggtccgact cgcgacggaa
     1801 cgggagtgtg ccgatttgtt tccagattgt gacgtgggga caatgccgcc attcggcaat
     1861 ctctatgggg tggaagtcta cgtcgatcaa ctcctgacga aggacgagtc gatcgtcttt
     1921 gaggcgggga cctatgtcgg agcgatgaag ctccggtaca aacattttgc cgaactggtc
     1981 cgtcccaagg tcgcggcgtt ccatcacgag ccatcaaagc tcgccccgta ttagcgtgac
     2041 gggacctcgt cccctggtgc gcaccgatg
//
LOCUS       DATDNW010000187         5039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_162305, whole genome shotgun sequence.
ACCESSION   DATDNW010000187 DATDNW010000000
VERSION     DATDNW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162305"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VI359_04600"
     CDS             <1..285
                     /locus_tag="VI359_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY6973562.1"
                     /translation="PMYSIAHALALVAVGRVQATITLEPENEWDLAASLLLIEESGGS
                     MTDRAGHPFHFNQPTPRFHGVCALSRSADQRLQTLLRSQMMGDGMPSQGR"
     gene            289..1155
                     /locus_tag="VI359_04605"
     CDS             289..1155
                     /locus_tag="VI359_04605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6973563.1"
                     /translation="MVTVALLGTGLLGRAIAERLHATHHSVIVYNRTAARALSLQTHG
                     VTVVTLPEQAIVQADVVLLVLADAAAIRAVLLTPAVAKVLRGKTVVQMGTIAPDESKA
                     IQREIERFGGAYCEAPVLGSLAEAKAGTLQVMVGGTGEQFSEWSPLFRSLSREPRLIG
                     PVGAAAALKLALNQLIAAEISAFALSLALCQRAGVPVDTFMTILRESALFAPSFEKKL
                     PRLLKRDYHNPNFSTRHLLKDVELFLAEAIKQRLAVSSVEGIVPILRNTIDHGLGEVD
                     YSAIYDIIHPLR"
     gene            1332..3083
                     /locus_tag="VI359_04610"
     CDS             1332..3083
                     /locus_tag="VI359_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY6973564.1"
                     /translation="MMKAIQKTIDPHAFDLLIDEGFIRQADLVKAIQDSMQARTELEG
                     VLIEKYRVPKAALGKALSRFFDCPYVPFDERTIADPKLVKDLSQDYLWKHRWLPLKCD
                     GEILDVLIDNPHDIERGHDIRRAFPGLTIRYAVSLRCDIQRFLQSIIGETDTGSITDT
                     LGELVQEARTEEASDLVSDGIDENDSAIVRLASQIIAEAYRHDASDIHIEPYTDRKET
                     AIRFRVDGTCSTYMRIPAAYRRALVSRIKIMANLDIAERRKPQDGKIRFRLNRDKDIE
                     LRVATLPTAGNNEDVVLRLLSAKEPIPLEAMELSPAMLSKLQEIVEKPHGIILCVGPT
                     GSGKTTTLHALLRHLNTDERKIWTAEDPIEITQEGLRQVQVNPKIGFTFAAAMRSFLR
                     ADPDVIMIGEMRDKETADVAIEASLTGHLVLSTLHTNSAVETVIRLLDMGCDPFNFAD
                     AIQGVMAKRLCKRICSNCKQTYHPSSQEYDKLVRAYGTNAWEALGISYHEEFQLYRGH
                     GCETCNQTGLKGRVALHELLCGSDELRSLIQNRAKTAEMLSVAIKGGMTTLVQDGIGK
                     VLAGLTTYDQVRAVAMK"
     gene            complement(3114..3434)
                     /locus_tag="VI359_04615"
     CDS             complement(3114..3434)
                     /locus_tag="VI359_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973565.1"
                     /translation="METRKNIRYAVQLPVSFNGDQVSGEGEGTILNLSADGCAITSTT
                     VAGPASYMQLTMQLREREEPVRVDLAAVRWSTPARFGVEFIKVRPEEGDRLKRFVKAL
                     ETGV"
     gene            complement(3479..3970)
                     /locus_tag="VI359_04620"
     CDS             complement(3479..3970)
                     /locus_tag="VI359_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973566.1"
                     /translation="MSGLQLSLFIRRIGLLALSCAVLGVSACTHPAIQRVGSTELVPS
                     PDRSLPSDRRNLAGEWEYEDGAVFVLTLDKQGNGPYAWKDGRIETQMLTDRTWKGMWF
                     QKENDREGGFSVEFSPDFSEGDGTWWYTRIGDDQTPSQKGGTFHLTRKSLSAKVSETP
                     PAP"
     gene            complement(4074..>5039)
                     /locus_tag="VI359_04625"
     CDS             complement(4074..>5039)
                     /locus_tag="VI359_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6973567.1"
                     /translation="GLPELRQALASKLARYNGIKADPETEITISSGATGSFHCACLAL
                     LNPGDEVILFEPYYQYHISALLAVEAVPVIVKMTAPHWTFSAGEIERAMTHRTKAIIV
                     NSPGNPSGKVFSRTELEDIADVTRRHDLFAFTDEIYEYFLYDGSRHISLATLSDMSER
                     TITVGGYSKTFSITGWRIGYSVADQRWASLIGAMNDLLYVCAPAPLQAGVASGIEQLP
                     DSFYRDLAGDYQIKRDRICHALEKAGLTPSIPQGAYYVLADVSSLPGKTGKERAMHLL
                     NKTGVAGVPGEAFFSGDEGRYFSRFSYAKTDSELDEACRRLARIG"
BASE COUNT         1118 a         1474 c         1356 g         1091 t
ORIGIN      
        1 ccgatgtact ccatcgccca tgcactcgcc ctcgtggccg tgggtcgcgt tcaggccacg
       61 atcacgctcg aaccggaaaa cgaatgggat cttgccgcca gtctgttgtt gatcgaagag
      121 agcggtggga gcatgaccga cagggcggga catccatttc atttcaacca gccgacaccc
      181 agattccacg gcgtgtgtgc cctgtcacgg tcagcggatc agaggctaca aaccctcttg
      241 cggtcgcaga tgatgggtga tggcatgcca tcgcaaggaa ggtgaattgt ggtgaccgtt
      301 gcgctgctgg gaaccggcct tttgggtcga gcgatcgcgg aacgactcca cgctacgcac
      361 cattctgtga ttgtctacaa ccggactgct gcccgggcac tttccctcca gactcatggc
      421 gtgaccgtcg tcacgttgcc cgaacaagcg atcgtgcaag ccgacgtggt cctcctggtg
      481 ctggccgatg ctgcggctat ccgagcggtc ttgctcacac ccgcagtcgc caaggttttg
      541 cgaggcaaaa ccgtcgttca aatgggcacc atcgctccag acgagagcaa ggcgattcag
      601 cgcgagatcg aacgattcgg cggcgcatac tgcgaagccc cagtcctggg cagtctcgct
      661 gaagcgaaag ccggcacact gcaggtcatg gtgggcggca cgggcgaaca gttctctgag
      721 tggtctccat tgttccgttc gctcagccgt gagccccgtc tgattggccc ggtcggagcg
      781 gccgccgcac tcaaactggc cttgaatcaa ctgatcgcgg cggagatatc ggcattcgca
      841 ttgagccttg ccctgtgcca gcgagccggg gttcccgtcg acaccttcat gacgattctg
      901 cgcgaaagcg ccctctttgc cccctctttc gagaagaaat taccccgcct cctcaagcgg
      961 gactaccata accccaattt ttcgacccgg catcttctga aggacgtcga actgttcctt
     1021 gccgaggcga tcaagcagcg actcgccgtc agcagtgtcg aaggaattgt tccgatcttg
     1081 cggaatacga tcgaccatgg gctgggagag gtcgattatt cggcaattta cgacatcatc
     1141 catccgcttc gatagcttgc cttatcttca tgcccacatt tccgtcccaa agggcagaca
     1201 atgttgtgtg gaatcctgcc ccgtttttga aattcattgt gcgcgatggg aaaatctcta
     1261 tactcaattc catgagcatc ccgccgaccc agtaactgca catttctcag agaattcgtg
     1321 gaataacacc gatgatgaag gcgattcaga aaacaattga tccccatgct tttgatctct
     1381 tgattgacga ggggttcatt cgtcaggccg atctggtgaa ggcgatccag gattctatgc
     1441 aagcacgcac cgagctggaa ggcgtgctga tcgagaaata ccgcgtccct aaggcggcgc
     1501 tcggcaaggc gttgagcagg tttttcgact gcccctatgt tccgttcgac gaacggacca
     1561 tcgctgatcc caaactcgtg aaagatctca gccaagatta cctgtggaaa catcgctggc
     1621 ttcctctcaa atgcgatggc gagattttgg atgttctgat cgacaacccc cacgatattg
     1681 agagaggtca cgacattcgc cgcgcctttc ctggtctgac gatacggtac gccgtgagcc
     1741 tgcggtgtga catccagcgg tttctccagt cgatcatcgg agaaaccgac accgggtcca
     1801 tcaccgacac ccttggtgag ctggttcaag aagccagaac cgaagaggca tccgatctcg
     1861 tctccgacgg cattgatgag aacgattcag cgattgtacg gcttgccagt cagattattg
     1921 ccgaggccta tcggcacgac gcatccgata ttcatatcga gccctacaca gatcgcaaag
     1981 aaacggccat ccgtttccgt gtggacggaa cctgctccac ctatatgcgg attccggccg
     2041 cgtatcgtcg agcgctggtg tcccgtatca aaatcatggc caatttggat attgccgaac
     2101 gccgcaaacc ccaggacggg aagatccgat tcaggctgaa tcgggacaag gatatcgaac
     2161 tgcgcgtcgc cacactgccc accgccggca acaacgaaga tgtcgtccta cggctgctct
     2221 cggcaaagga acctatcccc ctcgaggcca tggagctttc ccctgccatg ctcagcaagt
     2281 tgcaggagat cgtggagaaa ccccatggga tcatcttgtg cgtcggcccc accggttccg
     2341 gaaaaacgac gacccttcac gctttgcttc gccacctgaa caccgacgag cggaaaatct
     2401 ggaccgcgga agatcctatt gagatcacgc aagaaggtct ccgacaggtg caggtcaatc
     2461 cgaaaattgg attcaccttc gctgccgcca tgcgatcatt cttgcgagcc gacccagacg
     2521 tcatcatgat cggcgagatg cgagacaagg aaacagccga tgtggctatc gaggcctcgt
     2581 tgaccggcca ccttgtcctg agcaccttgc acacgaacag tgcggtcgag acggtgatcc
     2641 gacttctcga tatgggatgc gatccgttta atttcgccga tgccatacaa ggcgtgatgg
     2701 ccaaacgtct gtgtaagcgg atctgttcca attgcaaaca gacctaccat ccatcgtccc
     2761 aagaatacga caaactcgtc cgcgcatacg gcacaaacgc gtgggaagcg ctaggaatta
     2821 gctatcatga agaatttcag ctctatcgtg gacatgggtg cgagacctgc aatcaaacag
     2881 gcctcaaagg gcgggtcgcg ttgcacgaac tcttatgcgg atcggatgag ttgcggagcc
     2941 tgattcagaa ccgagccaag accgctgaaa tgttgagcgt tgcgattaaa ggaggaatga
     3001 caacactcgt acaggacggg attggaaaag tgctcgccgg cttgacgacg tacgaccagg
     3061 tcagagccgt cgccatgaaa tgatcgacct cgtatggtat cggctgtcta ggctcagacg
     3121 cctgtttcta acgccttcac aaatctcttc agccgatccc cctcttcagg acgaaccttg
     3181 ataaactcaa cgccaaatct cgccggagtc gaccatcgaa ccgccgccag atcgacacgg
     3241 accggctctt cccgctcccg gagttgcatc gtcaactgca tgtaggacgc cggaccggca
     3301 acggtcgtcg aggtgatggc acagccgtcg gcggacaaat tcaaaatagt cccttcgccc
     3361 tctcccgata cctggtctcc attgaacgat acgggaagtt gaaccgcata ccttatgttt
     3421 ttacgtgttt ccacgagccc tctgctgcaa accgactgat cgtcctagat gctccctatc
     3481 acggagccgg cggtgtctcg ctcaccttcg ccgagagaga ttttctggtg agatgaaacg
     3541 tgccgccctt ttgtgaagga gtctgatcgt caccgatgcg cgtgtaccac catgtgccgt
     3601 ctccctctga aaagtcaggc gaaaactcga cgctaaaccc gccttcgcgg tcattctcct
     3661 tttggaacca catccctttc cacgtccggt cggtgagcat ctgcgtctca atgcgcccgt
     3721 ccttccaggc gtagggtcca tttccttgct tgtcgagtgt gagcacgaat accgctccat
     3781 cttcatattc ccattctccg gccaggttcc ggcggtcgct cggcagggat ctatcaggcg
     3841 aaggaaccag ttcggttgaa cccacccgtt gaatcgcagg atgagtacag gcgctgacac
     3901 ccaacactgc acacgaaagc gccaggagac caatgcgccg tatgaatagg gacagctgta
     3961 aaccactcat ggttgttgta cttaccatgt ggaagccgga tgcacaagca gatgaatcga
     4021 actgactgtg cggtgatgcg cacagcgcgc tgaatcccat tttggtgaat ctctcaccca
     4081 atccgcgcca gtcgccggca ggcttcgtcg agttcggagt ccgtctttgc ataactgaac
     4141 cggctgaagt agcgcccctc gtcccctgag aagaatgctt ctccgggcac gcccgcgacg
     4201 ccggtcttgt tcaacaaatg catcgcccgc tctttgccgg tttttcctgg caggctcgag
     4261 acatcggcca atacatagta ggccccttgg gggatcgacg gtgtgaggcc ggccttctca
     4321 agcgcgtggc agatgcgatc ccgctttatt tgataatccc ctgcaaggtc tcgataaaac
     4381 gagtctggga gctgctcgat ccccgatgcc acaccggctt ggagaggggc tggtgcacag
     4441 acatagagca aatcgttcat ggccccgatc agggaagccc atctttgatc cgcaacgcta
     4501 tatccgatgc gccagccggt gatgctgaag gttttggagt acccaccgac cgtaatcgtt
     4561 cgctcgctca tgtcggacaa tgtcgcgagg ctgatgtgtc ggctaccgtc atacagaaaa
     4621 tactcgtaaa tctcatcggt aaacgcgaac agatcgtgcc gccttgtcac atcggctatg
     4681 tcctcgagct cagtgcgaga aaacactttc ccggatgggt tgcccggtga attcacaata
     4741 atagccttgg tccggtgggt catcgcgcgt tcaatctcac cggccgagaa cgtccagtgg
     4801 ggtgccgtca ttttcacaat gaccggtacc gcttcaacag ccaacagtgc actgatgtga
     4861 tattgataat agggctcgaa gaggatgact tcgtcaccgg ggttcagcag ggccaggcaa
     4921 gcgcagtgaa atgagccggt agctccggaa ctaatcgtga tctccgtttc tgggtctgcc
     4981 ttgatgccgt tgtaccgagc cagctttgat gcgagtgctt gccgaagctc cggtaggcc
//
LOCUS       DATDNW010000188         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_23362,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000188 DATDNW010000000
VERSION     DATDNW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23362"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VI359_04630"
     CDS             complement(<1..239)
                     /locus_tag="VI359_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY6973568.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVEAKAGSITEMIETLNSAHPGLKDR
                     LCDDTGELRRFVNIYVNEEDIRFLKGKDTSLKDGD"
     gene            complement(236..1486)
                     /gene="thrC"
                     /locus_tag="VI359_04635"
     CDS             complement(236..1486)
                     /gene="thrC"
                     /locus_tag="VI359_04635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY6973569.1"
                     /translation="MAKMKALVCRECGKEYPTKAIHVCEMCFGPLEVKYNYEEIKKSI
                     SRKTIEEGPRSMWRYIDLLPVEGTNFVGPHAGLTPMVRAKNLGAYLGLDELYIKNDTV
                     NHPTLSFKDRVVAVALTRARELGFETVACASTGNLANSVSAHAAAANLHCYVFIPGDL
                     EAAKVLGNLIYKPHVVEVEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWKTPDQVVIPMASGSLLTKIWKGLNEMHALGLVDSVRTKINGAQAEGCSPI
                     STAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESKGSMDMVPDDEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLKKLVKQGTIKKHDVTVAYITGNGLKTQEAVIDAVGRP
                     VRIQPSLVSFEKTFKMGKNGGGDT"
     gene            complement(1514..2326)
                     /gene="moeB"
                     /locus_tag="VI359_04640"
     CDS             complement(1514..2326)
                     /gene="moeB"
                     /locus_tag="VI359_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY6973570.1"
                     /translation="MEFTEEQINRYSRHILLPEVGGKGQKKLAKAKILIVGAGGLGSP
                     AALYLAAAGVGTLGLIDSDVVDLTNLQRQILHHTSDIGRPKVVSGKEKIQALNPDVSV
                     SMYEERLTAGNALKIISDYDVVIDGVDNFTAKFLINDACFFADKPLVHGGILRFDGRV
                     TTIIPKKSACYRCVFKTPPPPGLVASCQEAGVIGVLAGIIGTIQATEALKLVLGIGHP
                     LTNRMLDFDARKTQFREIKVRRNPTCALCSDHPTITELFDDGDPYAGCAVHP"
     gene            complement(2425..3354)
                     /gene="cysK"
                     /locus_tag="VI359_04645"
     CDS             complement(2425..3354)
                     /gene="cysK"
                     /locus_tag="VI359_04645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY6973571.1"
                     /translation="MTMVLHKEITELIGKTPLVRLNRLSPPDSATIYGKVEFFNPGGS
                     VKDRICLNMINEAERQGRLKPGGTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTPAWEGMKGSIREAESIIAQNPSYFMPDQFSNPANPAMHRMTT
                     APEIWEALGGKIDAFVAAVGTGGTITGCGEVFKEKNPHVKIIAVEPAASPVLSGGDPG
                     PHKIQGIGAGFVPKVLNQRILDRVITVTDDEAYQTAKALSKKEGLLVGISAGANVFAA
                     QKVAQELGPGKNVVTVLCDTGERYISIEKYFNI"
     gene            complement(3351..>3567)
                     /gene="thiS"
                     /locus_tag="VI359_04650"
     CDS             complement(3351..>3567)
                     /gene="thiS"
                     /locus_tag="VI359_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY6973572.1"
                     /translation="QFRSGMQVKINGKMENIQGGTILDLLKAKNIEPQMVAIEVNDAM
                     LERNHLATTTLKEGDQIEFLFYMGGGR"
BASE COUNT          690 a         1023 c          982 g          872 t
ORIGIN      
        1 tcgtcgccgt ctttgagtga agtgtctttc cctttgagga aacggatatc ttcttcgttg
       61 acgtagatgt tgacgaagcg ccgaagctcg ccggtatcat cgcagaggcg atccttcagg
      121 cccggatggg cgctgttgag cgtctcgatc atttccgtga tactgccggc cttggcttcg
      181 acttcccctt ggcctttcgt caacgggcgc aagggagtcg ggatgcggac tttaatcatg
      241 tgtcaccacc accgtttttc cccatcttga aagttttctc aaagctcacc aggctgggtt
      301 gaatccggac cgggcggcct acggcgtcga tcacggcctc ctgggttttc agcccgttgc
      361 cggtgatgta ggcgaccgtc acgtcgtgtt tcttgatggt gccctgtttg accagcttct
      421 tgagcacgcc gatcgtgacg ccgccggccg tttcggcgaa aatgccttca gtctgggcaa
      481 ggagcttgat cccctcgacc acttcgtcgt cggggaccat atccatcgag cctttgcttt
      541 ccgctgtggc tttcaaggca tagtaaccgt cggcaggatt gccgatggcc agagattttg
      601 cgatggtttt cggtttgacc ggcttgaaga aatcacggcc ggccttgaag gcggtcgaga
      661 tcggcgagca cccttccgct tgtgctccgt tgatcttggt ccgaaccgaa tcgaccaagc
      721 cgagggcgtg catttcgttc agccccttcc agatttttgt gaggagggag ccggaggcca
      781 tcgggatcac gacttggtct ggcgtcttcc aaccgagctg ctcgacggtt tcgaaggcaa
      841 gcgtcttgga tccctctgca taatagggtc ggatgttgat gttgacgaag gcccagccgt
      901 gctcacccgc aatctcgctg cagagccggt tcacgtcgtc gtaatttcct tcaacctcga
      961 cgacgtgcgg cttgtagatc aagttgccca acactttggc tgcctccaga tctcccggga
     1021 tgaacacgta acaatgcaga ttggccgctg ccgcatgggc cgagacagaa ttcgccaggt
     1081 tgccggtcga agcgcaggcc accgtttcaa atcccagttc gcgggcacgc gtgagcgcca
     1141 cggccacgac tcgatccttg aacgagagag tcgggtggtt taccgtatcg ttcttgatat
     1201 agagttcgtc cagtcccaaa taggcgccga gattcttcgc gcgtaccatg ggcgtcagtc
     1261 cggcatgagg tccgacgaag ttggtgcctt cgaccggcag caggtcgata taccgccaca
     1321 tgctgcgagg tccttcttcg attgtcttac gcgagatgga cttcttgatc tcctcgtagt
     1381 tgtactttac ttcaagagga ccgaagcaca tttcgcacac gtggatggct ttcgtcgggt
     1441 attcttttcc gcactcgcgg cagaccagcg ctttcatctt ggccatgatc acaccacctt
     1501 gagaatgctg tcgttaagga tgcactgcac agccggcata cggatctcca tcgtcgaata
     1561 gttcggttat cgtcggatgg tcactgcaga gcgcacaggt cggattacgg cgcaccttga
     1621 tctccctaaa ctgtgtcttg cgcgcatcga agtcgagcat gcggttggtc aaggggtgac
     1681 cgatccctaa taccagcttc agagcttcgg ttgcttgaat cgttccgata atccccgcga
     1741 ggacgccgat gacacccgct tcctggcacg acgcgactag tccgggcgga ggcggagttt
     1801 tgaatacgca tcgatagcac gccgatttct tcgggatgat cgtggtgacg cgtccgtcga
     1861 accgcaaaat gcctccgtgg accaacggct tgtcggcaaa gaagcaagca tcattgatga
     1921 ggaacttggc ggtaaaatta tccactccgt cgatcacgac atcataatca ctgatgatct
     1981 tgagcgcatt gccggcggtg agccgctctt catacatcga gactgatacg tcgggattga
     2041 gagcctgaat cttttctttc ccggagacga ccttgggtcg gccgatatcg gaagtatgat
     2101 ggagaatctg acgttggagg ttcgtcaggt cgacgacgtc gctgtcgatc aagccgagcg
     2161 taccgactcc ggccgcggcc agatacaggg cggcggggga gccgagtcca ccggcgccga
     2221 cgatcaaaat cttcgccttc gccagcttct tctgcccctt gcccccgact tccggcaaca
     2281 ggatgtggcg actgtatcta ttgatttgct cttcagtaaa ttccatgact ttgtccaggc
     2341 tagtggttag aggcacgggg ccagaggtat ttcgagaccg gttgctcctt tgccgcctct
     2401 cgccactcgc ctcgtgcctc gcacctaaat attaaagtac ttctcgatac tgatataccg
     2461 ctcgccggta tcgcataaca cggtcacaac attcttgccc ggaccgagtt cctgcgcaac
     2521 tttttgggct gcaaacacat tggcgccggc tgaaataccg acgaggaggc cttccttctt
     2581 cgagagggcc ttggccgttt gataggcttc gtcgtcggtg accgtgatga cgcggtcgag
     2641 gatcctctgg ttcaaaacct tcgggacgaa tccagcgcca atcccttgaa tcttgtgggg
     2701 gcccgggtct ccgccggaaa gcacgggtga agcagccggc tcgacggcga taatcttgac
     2761 gtgtggattc ttttccttga acacttcgcc gcatccggta atggttccac ccgtgccgac
     2821 cgctgcgaca aacgcatcga tcttgccgcc aagggcttcc caaatttccg gcgccgtcgt
     2881 catcctatgc atagccggat tcgccggatt cgaaaattga tccggcatga aataggatgg
     2941 attctgggcg ataatgcttt ctgcttctcg aatcgaaccc ttcatcccct cccacgctgg
     3001 tgtcagcacg agttgcgcac cgtaagagga aaggaggctt gcccgttcca tactcatgct
     3061 ctccggcatg actaaaatca acttataccc gcgtaccgct gccaccaagg cgaggccgat
     3121 ccccgtattg ccgctcgtcg gttcgacgat cgttccgccc ggcttcagtc tgccttggcg
     3181 ttccgcctca ttgatcatat tgaggcagat ccggtctttt acgctgccac ccggattgaa
     3241 gaattcgacc ttgccataga tcgttgcgga atcaggaggc gacaagcggt tcagtctgac
     3301 gagcggggtc ttcccgatca gctcggtaat ttccttatgg aggaccatcg tcatcggccg
     3361 ccacccatgt agaacaagaa ttcaatctga tcaccctctt tgagggtggt ggtggcgaga
     3421 tggttgcgct ccaacatcgc gtcgttcacc tcgatggcga ccatctgcgg ttcaatgttt
     3481 ttagccttga gaaggtcgag gatcgtacca ccctggatgt tctccatttt cccgttgatt
     3541 ttgacctgca tgccactcct aaattgg
//
LOCUS       DATDNW010000189         6672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1248109, whole genome shotgun sequence.
ACCESSION   DATDNW010000189 DATDNW010000000
VERSION     DATDNW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248109"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VI359_04655"
     CDS             <1..415
                     /locus_tag="VI359_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY6973573.1"
                     /translation="RHGTVGAVALDGWGTVASGASTGGIDSMLPGRVGDTPLIGCGVY
                     ADNRSGAVSMTGLGEGIICLAMAKTVCDRLASRISPAVAAQQISGQLVSRLQGAAGML
                     VLTSNGRFAIRHVTPCMAAGWWDGKGKPTVRGRFR"
     gene            412..840
                     /locus_tag="VI359_04660"
     CDS             412..840
                     /locus_tag="VI359_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6973574.1"
                     /translation="MSDSIFHLAFPIHDVAAAKRFYVEGLGCTLGRESPTAVTFGLAG
                     HQLVAHLVPELQPTQQGIYPRHFGLVFLSKTRWQELADRAKAKGLPFYQQPRVRYPGT
                     RIEHHTFFIEDPSRNLLEFKYYTFESAIFGEHGFTQVGDR"
     gene            complement(869..2914)
                     /locus_tag="VI359_04665"
     CDS             complement(869..2914)
                     /locus_tag="VI359_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6973575.1"
                     /translation="MSSRPVIALWLVCASSLLVTTAELVRAGEEEADIALGEILISET
                     RTPQLAHQLAGTVTLIAREQIDRSPYPGGHQIDDLLRSVPDVQPSLLSSRYNHPTAQA
                     VSLRGLGARRSLVLLDGVPLNDGFGGWINWGLVPDRVQRIEVVPGGASSLWGTWAMGG
                     VIHIITEPAATGSGGKLESQAGNRDTYTQALSVHTGTDRLNLTLGYRWYHSNGYITVP
                     SYQRGPIDRTDDSRHEHFSGAVSTALDSRTTLTLNGTLFREDRSFGTPQSLATRTIGM
                     LSLGLTGEPLEGQRYEAKIFGQWQTFRNLTSQVVPSPTVRLSEFQDTIQTIPSNDFGA
                     SAQGTSRISPAHTLVLGGDIRTIIAQSGEDLFTATGPAGSTLARGQQLGGGPFAEWIY
                     RPTEQLTITPSVRADWWKQFNAQVVSSTGIITLPDDRTISVVNPKLSALYRVEEWLRV
                     GTSVYQGFRAPTLNELYRSFGFGGFTFAANDRLAPERLTGAEGKIEGELPGIPDLSWR
                     VSGHFDQVKDQILFVTQSALLAQRQNIGRTRTIGTEVDVRWRVAPLLAATVGYAYADS
                     TITSFPGNPSREGNIVPNVSRHQVVMGVIVGRPDIVEVTIQGRYLSRQYADDANQQPI
                     GDFVVVDASIQKPIAKGIRMFFNGENLTNQQYIAAQTGPVKTLGVPLLVMGGIRMEH"
     gene            complement(2925..3659)
                     /gene="mtgA"
                     /locus_tag="VI359_04670"
     CDS             complement(2925..3659)
                     /gene="mtgA"
                     /locus_tag="VI359_04670"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY6973576.1"
                     /translation="MPKSTSQRRARLILSVILLVCLPIGAVLLVWLVTLPNVSALKTT
                     NPSITALMEMRQAQAQDRGQTAGRQWQWVPLARISPHLRRAVVAAEDASFFTHEGFDW
                     EGIKDAAIANLEAGELKRGGSTITQQLAKNLYLSSERSMFRKAREALITRLLEHHLSK
                     DRILELYLNVAEWGIDVYGAEAAARHHFQKSSHDLTAEEAAWLAAILPSPRRYDPIRK
                     SAALSRRYERIIYRMNKYPSSNRTSH"
     gene            3761..4363
                     /gene="yihA"
                     /locus_tag="VI359_04675"
     CDS             3761..4363
                     /gene="yihA"
                     /locus_tag="VI359_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY6973577.1"
                     /translation="MKILNAEFIRSCLSAEQLPAGAIPEFAFAGRSNVGKSSLINTLL
                     QRKGLAKVSRTPGKTRALNLFAVSTDDPAMPRCHIVDLPGYGFAKVSKTVRAQWAPMI
                     EGYLASREQLLTVVLLIESRVPREQDGETIAWLASIGRPPIVVATKADKLKASERVRA
                     LRSLGDVLAIHDRETLILFSSVTGEGRDRLWAALKQRVRT"
     gene            complement(4287..5309)
                     /locus_tag="VI359_04680"
     CDS             complement(4287..5309)
                     /locus_tag="VI359_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY6973578.1"
                     /translation="MMDRSTNAPHRRRLPVATCVFTLLLGFSSLEAAQVEDRIVAVVN
                     SDLIMMSEMKRELAPERERLKKLHRGQELARRIKIAESMALTTMIERKIQLQEAKARG
                     VEVSDQEVKQTIKQMKLQGEKIDETDAWTFKNIRDQLTLLRLVDREVRSGVMVVDTDM
                     RRYYQEHRDRFALPEEYTLSQILLERRSSDDGAAAKARAREIIAMLKQGETFEDLALR
                     YSEGPNASRGGALGLVRQGELLPAIERAIANLEPGGISDLIDAPEGIHIVRVEEKQPK
                     QFRPYEAVKTEIQGLVFQQKSEDVFQAWLANLKNKAYIEVKFEPAASGQPTGGLSPLP
                     SPMKKE"
     gene            complement(5439..6335)
                     /locus_tag="VI359_04685"
     CDS             complement(5439..6335)
                     /locus_tag="VI359_04685"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY6973579.1"
                     /translation="MNRSRIAVVSTGYARLSLLFAGLIGSVMLLASCTPPPEDPVLAL
                     VNGRQITQSEFDIRWSELADATRARYEKEGGKRRFLDELITRELLMQEARKKGLDQDD
                     TIRERTQRYKEQLILDELLKDKIKAKIELTKEELDAYYEKHARELLTPLKVSVSQMLL
                     PNYPAAKDLEKQVNQGGDFAKFAQRYSIDGKTKANGGDLGPYRKGLVIPEVDEVIHTL
                     KPGMISAPIKTDAGYYLVKIVPLDKEVIQADLATRERLRQELLNEKRRKRFDEMIADI
                     RGRAIVRLADASRYVADDVGKR"
     gene            complement(6357..>6672)
                     /locus_tag="VI359_04690"
     CDS             complement(6357..>6672)
                     /locus_tag="VI359_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY6973580.1"
                     /translation="LPPEPVERLFEVMQIRLQAKALRLASVELKTQSVVITLDAKNPV
                     GSSGIQRLMDRHKKRLRFLSPLSFELQMPHQDWSSIFRELTATLQSLNVCDTNTSESA
                     QG"
BASE COUNT         1311 a         1877 c         1991 g         1493 t
ORIGIN      
        1 tcggcatggc acggtgggag ccgtggcctt ggacggatgg ggaacggttg catctggggc
       61 gtccacagga ggcattgatt caatgctccc agggcgagtc ggcgacacgc ccctgatcgg
      121 ctgcggagtt tatgccgaca atcgcagcgg ggccgtatcc atgacgggac tcggtgaagg
      181 catcatctgt ctcgccatgg ccaagaccgt ctgcgatcgg ttggcctccc gtataagtcc
      241 cgcagtggct gcccaacaga tctcggggca gttggtatcc aggctccagg gggcggcagg
      301 catgttggtt ttgacgtcga acggccggtt cgctattcgt catgtcacgc cttgcatggc
      361 ggcaggatgg tgggacggga aaggaaagcc gacagtccga gggcgatttc gatgagtgac
      421 agcatctttc atctggcatt tcctatccat gatgtggcgg cggccaagcg tttttacgtt
      481 gagggacttg gctgtacgct cggccgtgaa tcaccgaccg ccgtgacgtt tggcttggcc
      541 ggccatcaac tcgtggcgca tctggtgccg gaactgcagc cgacccaaca ggggatctac
      601 ccgcgccatt tcggcctggt ttttctctcg aaaacacgct ggcaggaatt ggccgatcgt
      661 gcgaaggcaa agggactgcc cttctatcaa cagccgcggg tacggtaccc tgggacccgt
      721 atcgagcacc acacgttctt catcgaagat ccctcgcgca atctactcga gttcaaatac
      781 tacacatttg aatcggccat ctttggcgag cacggattta cccaagtcgg agaccgctag
      841 gctagaatcg atccatcctg gcgcgccatt aatgctccat gcggatccct cccatcacga
      901 ggagagggac gccaagggtc ttcactggtc ccgtctgcgc ggcgatatac tgttgattcg
      961 tcaggttctc gccattgaag aacattcgaa ttcctttcgc aatcggtttt tgaatcgagg
     1021 catcgaccac gacaaagtct ccgatcggct gctggttcgc gtcgtcggca tattgtcgcg
     1081 acaagtaccg gccttgaatc gtgacctcaa caatatcggg ccgccccacg ataaccccca
     1141 tcaccacttg gtgccgcgac acattcggaa cgatattgcc ctctcgactc ggatttcccg
     1201 gaaacgatgt aatggtggaa tcggcgtagg cgtatcccac ggtcgcggcc aggagtggag
     1261 caactcgcca cctcacatcg acttcagtgc cgatcgtgcg tgtgcggccg atgttctgcc
     1321 gttgagccag tagagcactt tgcgtgacaa agaggatttg atccttgacc tgatcgaaat
     1381 ggccgctcac cctccaggac aggtcgggaa ttccgggcaa ctctccttca atcttccctt
     1441 cggctccggt cagccgctct ggggcgagac gatcattggc ggcgaacgta aatccgccga
     1501 agccgaagct tcgatacagt tcgttcaacg tgggggctcg aaatccctgg tagaccgacg
     1561 tgccgacacg aagccactct tcgacccggt agagggctga taattttgga ttcacgaccg
     1621 agatcgtgcg atcgtcaggg agcgtgatta ttccggtgct cgacacaacc tgagcattaa
     1681 actgtttcca ccaatctgct cgcacgctcg gcgtgatggt cagttgctcg gttggtcggt
     1741 agatccattc agcaaagggg ccaccgccga gctgctggcc acgcgcgagg gtcgatccgg
     1801 ctggccctgt cgccgtaaaa aggtcttcgc ccgattgcgc gatgatcgtc cggatgtctc
     1861 cgccgagcac cagggtatgc gccggtgaga tccggctcgt cccttgagcc gacgcgccga
     1921 aatcgttgct gggaatggtc tggatcgtgt cttggaactc gctgagtcgg actgttggag
     1981 atgggacaac ttgcgacgtc agattccgaa aggtctgcca ttgtccaaag atcttggcct
     2041 cgtaccgttg gccctcaagg ggttctcccg tgagtcccag cgacagcatc ccgatcgtac
     2101 gcgtcgcaag actttgggga gtcccaaagg aacggtcctc acgaaacagc gtgccgttga
     2161 gcgtgagcgt agtccgcgaa tcgagcgcgg ttgacaccgc ccccgaaaag tgctcgtggc
     2221 gagaatcgtc cgtccgatcg attggacctc gctggtagct cggcaccgtg atatatccgt
     2281 tgctgtggta ccagcggtaa cccagcgtca gattgagtcg atccgttccg gtatggacgg
     2341 acagggcctg ggtataggta tcgcggtttc ccgcttggct ttccaatttg cctccagatc
     2401 ctgttgccgc tggctccgtg atgatatgga tgacccctcc catggcccag gtgccccaca
     2461 gactcgaagc gccgcctgga acgacttcaa tgcgttgaac acgatccggc accaatcccc
     2521 aattgatcca gcctccaaac ccatcattga gaggcacacc gtctagcagc accaagctgc
     2581 gacgagctcc caggccccga agcgaaacag cttgagcggt gggatggttg tatcggctgc
     2641 tgagcaggct cggttggacg tccggtacag aacgaaggag gtcatcgatc tggtgaccgc
     2701 ccggataggg ggaccgatcg atctgttccc gcgcgatgag tgtgacggtt cccgcgagtt
     2761 ggtgcgccag ctgtggtgtg cgcgtttcgg agatgagaat ctccccgagg gcgatatcag
     2821 cctcctcctc gccggctctg accagttcgg cggttgtcac aaggaggctg ctggcgcaga
     2881 ccagccacaa cgcgatgacc ggccggctgc tcatgacgaa ggcctcaatg agaagtgcga
     2941 ttcgacgatg gatacttgtt catccggtag atgatccgtt cgtatcgtcg actcaaggca
     3001 gcagatttcc gaattgggtc gtaccggcgc ggtgagggca aaatggcggc caaccaggcc
     3061 gcttcttctg cggtgagatc gtgcgaagac ttttggaagt gatggcgcgc ggcggcttcg
     3121 gctccgtaca cgtcgatgcc ccactccgcg acgttgagat acagctcaag aatgcgatct
     3181 ttgctgagat ggtgctcgag gaggcgcgta atcagcgcct ctcgcgcctt gcggaacatc
     3241 gatcgttcgg aggacaggta cagattcttc gcgagctgct gcgtgatggt gctcccgcca
     3301 cgtttcaact cgccggcttc gaggttggcg atggcggcgt ccttgatacc ttcccaatcg
     3361 aatccttcat gcgtaaagaa cgacgcgtcc tccgcagcca cgacggcgcg acgaagatgg
     3421 ggtgagatgc gtgccagcgg cacccactgc cattgacgtc cggccgtctg accccgatct
     3481 tgagcctgcg cctgacgcat ctccatgaga gccgtgatcg aggggttggt cgttttcaag
     3541 gcagagacgt tgggcagcgt caccagccac accaacagca ccgcgccgat gggaaggcac
     3601 accaagagga tgacggagag aatcaggcgt gcgcgacgtt gcgatgttga cttcggcata
     3661 gtgtgatgcg tatgaacaaa ctcgaagctt gggacaacgg ggtcgatgag actttcgtac
     3721 ccgcgtgtcg cctcacgtta cctgttcttg agggttgccg ttgaaaatcc tgaatgcgga
     3781 gtttatcaga agttgcctga gcgcagaaca gcttccggcc ggcgcaattc cggaatttgc
     3841 ctttgcagga cgatcaaatg taggcaaatc gtccttgatc aataccctcc tgcagcggaa
     3901 gggtcttgcg aaagtgagcc ggacgccggg caagaccagg gcactcaatc tgtttgccgt
     3961 gtcgaccgac gatccggcga tgccacggtg tcatatcgtg gatctccccg gctatggatt
     4021 cgccaaggtt tcgaaaacgg tgcgcgctca atgggcaccc atgatcgaag gctatcttgc
     4081 cagtcgggag caattgctca ccgtggtgtt gttgatcgag agtcgagtgc cgagagaaca
     4141 ggatggcgaa accatcgcct ggctggcgtc catcgggcgt ccgccgatcg tggtcgccac
     4201 caaagcggat aaactgaaag ccagcgaacg agtccgtgcc ctgcgcagct tgggcgatgt
     4261 cctggccatc cacgatcgtg agacgctcat tcttttttca tcggtgacgg gagaggggag
     4321 agaccgcctg tgggctgccc tgaagcagcg ggttcgaact taacttcaat atacgctttg
     4381 ttcttcagat tcgccagcca agcctggaag acgtcctcgc ttttctgctg aaacaccaga
     4441 ccttggattt ccgttttgac cgcttcgtag ggtcggaact gtttcggctg cttctcctcc
     4501 acgcggacga tgtggatgcc ttcgggggca tcaatgaggt ctgagatacc gccgggctca
     4561 agattcgcga tggcccgttc aatggcaggc aacagttctc cctgtcgtac caaaccgaga
     4621 gctcctcccc gcgacgcgtt ggggccttca gaatatcgaa gtgcgagatc ctcaaaggtt
     4681 tctccttgct tgagcatcgc gattatctcg cgggccctgg ctttcgcggc ggcaccgtcg
     4741 tccgaggatc ggcgttcgag caggatttgg ctcagcgtat attcctcggg cagtgcaaac
     4801 cggtcgcgat gttcctggta ataccgtctc atatccgtat caacgaccat cacgccactg
     4861 cgcacttcac gatcgacgag tctgagcaac gtcagttggt cgcggatatt cttgaaggtc
     4921 caggcatcgg tttcgtcgat cttttcccct tgtagcttca tctgtttgat cgtttgcttg
     4981 acctcctggt cggagacttc aaccccgcga gccttcgctt cttgaagctg gatctttcgc
     5041 tcgatcatgg tcgtcaaggc catggactcg gcgatcttta tcctgcgggc caactcctgc
     5101 cctcgatgaa gtttcttgag tctctctcgt tcgggagcga gttctcgttt catctccgac
     5161 atcatgatca gatcggagtt gacgacggcg acgattcgat cttccacttg cgcggcttca
     5221 agagaggaga aaccgaggag aagagtaaag acgcaggtcg ctacaggaag gcgccgacga
     5281 tgtggcgcgt tggtggatcg atccatcatg tcacagatct cgaacgtccg cgatgcgaaa
     5341 tcgaacagcc catccaggct gctggatggt cggtggattg tacacgaatt caacgggaat
     5401 tgaggaggga gggctgacac gcgacaaaca ggcgaagttc agcgttttcc aacgtcgtcc
     5461 gccacatagc gcgaggcgtc ggcgaggcgg acaatcgccc tcccgcgaat gtcggcaatc
     5521 atttcgtcga accgcttgcg gcgcttctcg ttgagcaatt cctgccggag acgttccctg
     5581 gtcgccaggt ccgcctggat gacttccttg tcgaggggaa cgatcttgac gagatagtat
     5641 ccggcgtccg tcttaattgg cgcgctgatc atcccgggtt tgagggtgtg aattacctca
     5701 tcgacttcag ggatgaccaa gcccttgcga taagggccga ggtcgccccc attggccttc
     5761 gtctttccgt cgatggaata tcgttgggcg aatttggcga agtctcctcc ctggttgacc
     5821 tgtttctcta ggtcttttgc ggccggatag ttgggaagaa gcatctggga tacgctcacc
     5881 ttgagcggcg tcagaagctc gcgggcatgc ttctcatagt aggcgtccaa ttcctccttc
     5941 gtgagctcga tctttgcctt gatcttgtcc ttgagcagct cgtcaaggat gagttgttcc
     6001 ttgtatcgtt gcgtccgctc gcggatggtg tcgtcctggt ccaggccttt cttgcgggct
     6061 tcctgcatga ggagctcgcg cgtgatcaat tcgtcgagga agcgccgttt gccaccttcc
     6121 ttctcgtacc gagcgcgtgt tgcgtcggcg agttctgacc accggatgtc gaattcgctc
     6181 tgcgtgattt gtcggccatt gacaagtgca aggaccgggt cctcgggcgg gggagtgcag
     6241 ctggcaagca gcatcacact gccgatgaga ccggcaaaca gcagcgaaag acgagcgtac
     6301 cctgtactga cgacggcaat tcgagatcgg ttcataggtg taactggttc agcggcttaa
     6361 ccctgcgcag actccgatgt gttggtatca cagacattga ggctttgcaa ggttgcggtg
     6421 agttcccgaa agatggaaga ccagtcttga tgcggcattt ggagttcgaa tgagaggggc
     6481 gagagaaacc gaagccgttt cttatgtcga tccatcaggc gttggatgcc ggagcttcca
     6541 accgggttct tggcgtcgag cgtgatgacc acggactgtg ttttcaattc gaccgaggcg
     6601 agccggagcg ccttcgcctg gagcctgatc tgcatgactt caaacagtcg ttcgaccggt
     6661 tcaggcggta at
//
LOCUS       DATDNW010000190         4497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_292145, whole genome shotgun sequence.
ACCESSION   DATDNW010000190 DATDNW010000000
VERSION     DATDNW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292145"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VI359_04695"
     CDS             <1..226
                     /locus_tag="VI359_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973581.1"
                     /translation="ADLAVLDCSFPANRPGPVHLHAGECGQIAKEAGVGQLVLSHFYP
                     IAERYDVKSQAGEAYGGKILLARDRLNIRL"
     gene            complement(248..1102)
                     /locus_tag="VI359_04700"
     CDS             complement(248..1102)
                     /locus_tag="VI359_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY6973582.1"
                     /translation="MNESQIRALIRLLADEDEKIVRTISGKLVDIGTTAVPLLQEAEI
                     EQPEMAERIASVLEEIRGGRLEDELIQLSALPDEQMDLERGAFLIARYAYPTLDAPSY
                     GRQLDEMAQEVRERIGPRASGEETVKTLNRYLFTEQGFKGNTKNYYELENSYLNCVID
                     RRTGIPISLSVVYLLIGKRLGLPVHGIGMPGHFLVKYESDRYKIFIDCFNGGALLTEK
                     NCQRFLTEAGYGCDDKYLQHSPVRAILSRMIKNLLAIYSKLDDPIKKTRLTTFIELLG
                     AESKEGGL"
     gene            complement(1165..1944)
                     /locus_tag="VI359_04705"
     CDS             complement(1165..1944)
                     /locus_tag="VI359_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6973583.1"
                     /translation="MAQYWQEISALTMRWVLRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADAQVTQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     SSVILSRFIFVMTITSLQVLVILGVAFLFGVQPATGLLGVAVILLIGMLFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMGFLARFNPMTWAIDAVR
                     PLILSSWAEALPHVGMVVVVMVVFDALCLYGGAKAFRRAMG"
     gene            complement(1954..2925)
                     /locus_tag="VI359_04710"
     CDS             complement(1954..2925)
                     /locus_tag="VI359_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6973584.1"
                     /translation="MDRAVAIEVSHLSKTYDGHKAVEDLSFQVYRGEIFGLLGPNGAG
                     KSTTLRTLITLLRPTSGFARVLGHDTVKDADVVRHLIGYVPQERAIDRFLTGREHLQL
                     LAALYHLSKDEGARRIRELLKLVELEAHADRPAKTYSGGMKRKLDIACGLLPNPKILF
                     LDEPTLGLDVQSRLRIWDYVRMLKAQGMTVVMTTNYLDEADQLCDRLAIIDAGTIKTL
                     GSPTELKVGLGGDIVALTIHDANRLPVLAEALKGLSAIRAVNSTARGLDIRVESPEKV
                     LPAILEAASRLDCRVEFIEYHRPRLDDVFIAHTGRAITESVPEAETT"
     gene            complement(2925..3614)
                     /locus_tag="VI359_04715"
     CDS             complement(2925..3614)
                     /locus_tag="VI359_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6973585.1"
                     /translation="MQTDRERENQGREPQDKSLPFLIPARIPIFPLPNTVFFPKTYLP
                     LHIFEPRYRQMVTDAAMGGQCIGMVLLKEGWESDYYGNPPIFSMGCVGRIVSMQPLVD
                     GRSNILLQGLERYEIQAEHFDRSYREATIRLTPRPSDGRLDPMVRTKLVQSLEEFLKS
                     RDDAPTWQALFREEVSDDILINSVSTYLECTPLEKQFLLEAESLQQQARRLGDLLHFM
                     MHDPHGAKGWG"
     gene            3758..>4497
                     /locus_tag="VI359_04720"
     CDS             3758..>4497
                     /locus_tag="VI359_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEY6973586.1"
                     /translation="MTRINTGFIGCQRQVRRGSIPHAIVITLCIGTFVGGLLFAADPI
                     QEFRLATPGYHYVFPRDHGSHDDFRTEWWYYTGHLATKNGRRFGYQLTFFRRGIPRDQ
                     VKTLPSQWSITQLYLAHFAISDLAGEQFLYTEHISRAGLGKAGAERDRLHVWIDQWSA
                     ESPAAIPLTQNLRASNGTIGVQFTLSPEKPLVLHGLDGVSRKGSASGQASHYYSFTRL
                     ATTGTLSIGTEEFDVTGTSWMDHEFGSA"
BASE COUNT          984 a         1379 c         1211 g          923 t
ORIGIN      
        1 ggcggatctg gcggtactcg attgttcctt ccccgccaac cgaccaggtc cggttcatct
       61 acatgcggga gaatgcgggc agattgcgaa ggaggcgggg gtcggccagc tggtcctatc
      121 tcacttctat ccgattgccg aacggtacga cgtcaaatct caggcaggcg aagcgtatgg
      181 tgggaagatc ctcctggctc gcgaccggct gaacatcagg ctctaacagg tcggggtcag
      241 tcggcttcta caacccgcct tctttacttt ccgccccgag aagctcgatg aaggtcgtga
      301 gccgagtctt tttgatcggg tcatcgagtt tggaatagat cgccagtaga ttcttgatca
      361 tacgggatag aatcgcccgg acgggactgt gctggagata cttgtcgtcg catccatagc
      421 cggcttccgt gaggaagcgc tggcaattct tctcggtcag aagcgcgccc ccgttgaagc
      481 agtcgatgaa aatcttgtat cggtccgact cgtacttcac gaggaagtgg cccggcatgc
      541 cgatgccgtg gaccggaagg ccgagacgct tcccgatcag cagatacacc accgacaggc
      601 tgatcggaat ccccgttcgc cggtcgatca cacaattgag gtagctgttc tcgagctcgt
      661 agtaattctt ggtgttgcct ttgaacccct gctcagtaaa gagatagcgg ttcagcgtct
      721 tcacggtttc ttcgcctgac gcacgcggac cgatccgctc gcgcacctcc tgagccatct
      781 catcaagttg ccgtccatag gacggcgcat ccagcgtggg ataggcatag cgggcgatca
      841 agaaagcgcc ccgctcgaga tccatttgct catccggcag agccgagagc tgaatgagtt
      901 cgtcttcaag tctcccgccg cgaatttctt caagcaccga cgcaatacgc tccgccattt
      961 ccggctgttc gatctcggcc tcttgcagca atggcacagc tgtcgtcccg atatcgacga
     1021 gcttcccact gatcgtcctc acaatcttct cgtcttcgtc cgccaacaag cggatcaacg
     1081 cacggatttg gctttcattc accagcattg aagatcccag ctattcaccc acccgtctcg
     1141 ctcgctagtc tgttcaccgt cctgctatcc catcgcccgc cggaaagcct tcgccccacc
     1201 atataagcat agcgcgtcaa atacgaccat gacgaccacc accatcccca catgcggcag
     1261 agcttcggcc caactcgaga gtatgagcgg tcgcaccgca tcgatcgccc aggtcatcgg
     1321 attgaatcgc gccaagaaac ccatccagcc cggcatcgcc gccagcggca ccaacgccga
     1381 gctcaggaaa atcatcggga gcgacagaaa ccccagcacc gaaaagaagt cgccgtggct
     1441 ttttaccgaa aaggccatcg ccatcgaaat cgccgtcagt ccgaccccaa acaacatccc
     1501 tattaataat atcaccgcaa ctccgagaag tccggtcgcc ggctggacac cgaacaggaa
     1561 cgccacaccg aggattacca gcacttgcaa ggatgtgatc gtcatcacga agatgaaccg
     1621 gctcagaatg acggagctcc gatggatcgg cgtggacatc aaccgctcca agaatccgtt
     1681 ttccttatcg aagaggagat cgaccccacc ggccaagccg ttgttcagca cggtcatcac
     1741 tacgacgccc gccgcgagaa aactgatgta gttcggggct tgcgtcacct gggcatcggc
     1801 cgcccgctga aacaggttgc cgaaaaagat caaccaaaac agcatcggtt ggacaagcgt
     1861 gaagagcatg ctgaattttt cccggctcag ccgcagcacc caccgcatcg tcaaggcact
     1921 gatttcttgc caatactgcg ccactgctcc tccttacgtc gtttccgctt cgggcacgga
     1981 ttccgtaatc gcacgccccg tatgggcgat gaacacgtcg tccaatcgcg gtcgatggta
     2041 ctcgataaat tccacgcggc aatccaggcg gctggccgct tcaagaatgg ccggcaagac
     2101 tttttccggc gactcgaccc ggatgtccag cccgcgggca gtgctgttca ccgcacgaat
     2161 ggcggacagt cccttgagtg cctctgccaa gaccgggagt cggttggcat cgtgaatcgt
     2221 cagtgcgacg atgtcgccgc ccagccccac cttgagttcg gtcggggaac cgagcgtttt
     2281 gatcgtcccg gcgtcgataa tggccagccg gtcgcacagt tggtccgctt cgtccagata
     2341 gttggtggtc atcaccaccg tcatgccctg ggctttcagc atgcgcacat agtcccaaat
     2401 acgaagacga ctctgcacat ctagtccaag cgtcggctcg tccagaaaca gaattttcgg
     2461 attcggaagg agtccgcagg cgatgtcgag cttgcgcttc attccgccgg agtaggtctt
     2521 ggccggacga tcggcatggg cttcgagttc aacgagcttg agcaactctc tgatccgtct
     2581 cgcgccttcg tccttcgaca agtgatagag cgccgcgagc agttgcagat gttcacgacc
     2641 ggtcagaaag cgatcgatcg cccgttcctg cggaacgtac ccgatcaaat gccgcacgac
     2701 gtccgcgtcc ttcaccgtat cgtgccctaa gactctggcg aatcctgacg tcgggcggag
     2761 cagcgtgatc aacgtgcgga gggttgtgct tttcccggca ccgttaggcc ccagcaatcc
     2821 gaatatttcg cctcggtaga cctgaaagga gagatcctcg acggccttat gcccgtcata
     2881 ggtttttgac aggtgagaca cctcgatcgc gaccgcccga tccattaccc ccaacccttc
     2941 gccccgtgag ggtcgtgcat catgaaatgg agcagatcgc caaggcggcg agcctgttgc
     3001 tggaggctct cggcttccaa cagaaactgc ttttccaacg gcgtgcattc cagatacgtc
     3061 gacacggaat taatgaggat gtcgtcgctg acctcttcgc ggaataacgc ctgccacgtg
     3121 ggcgcgtcat cgcgtgactt gagaaactct tctaatgact gcaccagttt ggtgcgcacc
     3181 ataggatcga gcctgccgtc agatgggcgt ggcgttaacc tgatggtggc ctcacgatag
     3241 gaccggtcaa aatgctcggc ctggatttca taacgctcaa ggccttggag caggatattt
     3301 gaccggccat cgacaagtgg ttgcatgctc acgatccggc ccacacagcc catcgagaaa
     3361 atcgggggat ttccataata gtccgattcc caaccttctt tgagcaagac cataccgatg
     3421 cactggcccc ccatcgctgc atcggttacc atctgacgat aacgcggctc gaaaatgtgg
     3481 agcggcagat acgtcttggg gaagaagacg gtattcggaa gagggaagat cggaatgcga
     3541 gcgggaatga gaaacgggag cgatttatcc tggggttcac ggccttgatt ctcccgctcg
     3601 cgatcagtct gcatggctca cgatagccga tggttgccaa aattgtcaac ggcgcgagcc
     3661 cacggttgac gcggcccccg gcggagaaaa ccatgatcaa tacaggcgaa ttgtgcgatc
     3721 ggttcacgca tggtataagc ggcgtcgccg aatcatcatg acacgaatca acacaggctt
     3781 tatcggatgt caaagacagg tacgcagagg aagcatccct cacgcgatcg tcatcaccct
     3841 gtgcatcggc acattcgtcg ggggcctgct cttcgcagct gatccgattc aagagtttcg
     3901 cctggccacg ccgggctacc actatgtctt cccgcgcgat catggttctc acgatgactt
     3961 tcggactgaa tggtggtact acacgggtca cctcgcgacc aagaacggac gccggttcgg
     4021 ttatcaactg acattctttc gccgcgggat tcctcgcgat caggtcaaaa cccttccgtc
     4081 tcagtggtcc atcacgcagc tatacctcgc tcactttgca atcagtgacc tcgccggcga
     4141 acaattcctt tataccgaac acatcagtcg agccggcctg ggtaaagccg gcgccgagcg
     4201 ggatcggctg catgtctgga tcgatcaatg gagcgccgaa tcaccggcgg ccattccact
     4261 cacacagaac cttcgcgcgt cgaacggtac catcggggtc cagttcacgt tgtcgccgga
     4321 aaaaccgctc gtcctccatg gcctcgacgg cgtcagtcgc aaaggatccg cctccggaca
     4381 ggcatcccac tattattcct tcaccaggct ggccacgacc ggcacactgt cgatcggaac
     4441 cgaggaattc gatgtcacag gaaccagctg gatggaccac gagttcggct cggccga
//
LOCUS       DATDNW010000191         8065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_982035, whole genome shotgun sequence.
ACCESSION   DATDNW010000191 DATDNW010000000
VERSION     DATDNW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982035"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="ruvB"
                     /locus_tag="VI359_04725"
     CDS             <1..915
                     /gene="ruvB"
                     /locus_tag="VI359_04725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY6973587.1"
                     /translation="QERMKESLRICIEAAKQRQEALDHAIFYGPPGLGKTTIAHIIAR
                     EMGASLRSTSGLVLAHAGDLAAILMNLQEHDVLFIDEIHRLPASVEEALYPAMEDFQL
                     DLVVGQGPAARTVKLDLPRFTLVGATTRAGALTSPLRDRFGLVHRLEFYSPEELCTIV
                     TRSAGLLGIPIDRDGAAEIARRARGTPRIVNRLIKRVRDYAQVKAEGRITNMVAQEAL
                     VWLGVDTAGFDEMDRRILVTIIEKFGGGPVGVESLAAAVQEDKGTLEDVYEPYLIQAG
                     FLERTGRGRQVTKLAHDHFKVSTPFLFS"
     gene            1115..2191
                     /gene="pheA"
                     /locus_tag="VI359_04730"
     CDS             1115..2191
                     /gene="pheA"
                     /locus_tag="VI359_04730"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY6973588.1"
                     /translation="MAGDLSDYRKEIDRIDDEILRLLNERSKSVIEIGRLKKQKDADA
                     NLHTPAREAAIIDRLTHQSQGPFPTEAIRSVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGSSAQYVPVNSIKDVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEVLQEVSHHLLSKSGVADDVKKIYSHPHAIAQCRNWLETNLPHVPVSEVASTA
                     RAAEICVDDPSAGAIASELAAQLYGLKVIKSRIEDNMNNFTRFLVLSQKPPERTGKDK
                     TSLMLSVKDKVGALYDLLRPFASHGLNMTKIESRPSRRKAWEYIFFVDLEGHMEEERV
                     KKAIEEIKSRCLFMKVLGSYPSYN"
     gene            2196..3275
                     /gene="hisC"
                     /locus_tag="VI359_04735"
     CDS             2196..3275
                     /gene="hisC"
                     /locus_tag="VI359_04735"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY6973589.1"
                     /translation="MTIRVHPDIASLSPYVPGKPIEELQRELGLTRVIKLASNENPLG
                     PSPKALSVLSESASTLHRYPDGGAYRLREALADRWKVTPDHVILGNGSDEILGLLART
                     FLSPGDEAVMADQTFVIYKMEVTAAHGKPAVIPLKQWRHDLTAMADAITDRTRLLFVC
                     NPNNPTGTMVTGTDVARLMERVPSHVIVVFDEAYYEYVRSPQFPESMAYVKQGRNVIV
                     LRTFSKIYGLAGLRIGYGITTPEITNFLNRVRPPFNANSLAQRAALAALGDDEHVARS
                     RTVNGAGMGQMTAGLKALGFEAVPSEANFLYFDVGREGRGVFEGLLREGVIVRHIEGP
                     MLRVTIGLEEENQAFLEALKRVLKK"
     gene            3295..4308
                     /gene="aroF"
                     /locus_tag="VI359_04740"
     CDS             3295..4308
                     /gene="aroF"
                     /locus_tag="VI359_04740"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY6973590.1"
                     /translation="MIIVLKPEATEREVDHIIDRLRELGLKSQLSTGQERTIIGVIGD
                     DRILQNQPLTALPGVESVLPILAPWKLVSREFKREATIIDVGGVKIGGKKLAVMAGPC
                     AVERLELTVGIAHDVKAAGATILRGGAYKPRTSPYSFQGLGREGLDYLIEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKDLSHLPVIVDPSHATGR
                     WDLVAPMSKAAVAAGADGLLIEVHSNPECALCDGEESIRPNKFKELMQELRKIGEAVG
                     REV"
     gene            4361..5254
                     /locus_tag="VI359_04745"
     CDS             4361..5254
                     /locus_tag="VI359_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEY6973591.1"
                     /translation="MTVHFTQVTIVGVGLIGGSLGMILKRKGLADRVVGVGRRVENLK
                     KAVELGAIDRYVVDSTEGVREADLVVLATPVDTYERHLKEWASCLKTGAIVTDVGSVK
                     GPLVKQAEAALPHGVHFVGAHPIAGKEKTGVVAGTPQLFTGARCIITPTKTTDPEALE
                     RVRALWRETGSVVLEMDPYEHDKILGAVSHLPHVAAFALTNALIDLRERLIPSLDLAG
                     HSGGGLRDTTRIAASSPEMWRDIFLWNRDNIVACIEAYERSLARLKQLIKSGDAAGIE
                     KELERAKQEREKFPVQPPSNT"
     gene            5261..6583
                     /gene="aroA"
                     /locus_tag="VI359_04750"
     CDS             5261..6583
                     /gene="aroA"
                     /locus_tag="VI359_04750"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY6973592.1"
                     /translation="MASLTITPGRSLNGTISVPGDKSITHRAIILTALADGVSTVSKY
                     CRGEDCLNTIRACQVLGVRIEVSPETLRVHGKGLWGLSEPAAPIDCGNSGTGIRLLTG
                     LLAGQDFFTVLTGDESIRRRPMGRVVKPLREMGAMIAGRKGGELAPLAVTGSKLRAIA
                     YASPVASAQIKSSLLLAALFAQGTTRITEPRLSRDHTERMFQYFGIPLCREGLTVSLD
                     GRPTVGWNAVPELIVPGDLSAAAFFLVGASIVPGSDIMIEGVGVNSTRTGLLEVLARM
                     GADIQLLNRREKAGEPVADLRVKSAALRGVTIGSDLIPQTIDEFPILCVAAAVAEGQT
                     VISGAEELRVKESDRIATMAAELRKLGVQVTERPDGMTIQGVGKANENGRLTAATCQS
                     HGDHRVAMSLAIAGLTASTNTVIDDTACIETSFPNFQSKLLELLTKDA"
     gene            6630..7313
                     /gene="cmk"
                     /locus_tag="VI359_04755"
     CDS             6630..7313
                     /gene="cmk"
                     /locus_tag="VI359_04755"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY6973593.1"
                     /translation="MKGGKLNQMIIAIDGPAGVGKSTVAKLLAARLGYLYLDTGALYR
                     AVAWNVLHHRVDPAESQAVESLLSGTSIQMLFQNGSAEVLVNGKNITHELRTPAVSAA
                     ASIVSAIPAVRAWLLPVQRQIGSAGAVVAEGRDIGTKVFPQADVKFFLEADADVRTQR
                     RHRELVAAGHTGALEQTNADLIGRDTRDRTRAIAPLVAAEDALHIDTSALTVEAVVDH
                     MMVVITAKL"
     gene            7310..7966
                     /locus_tag="VI359_04760"
     CDS             7310..7966
                     /locus_tag="VI359_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY6973594.1"
                     /translation="MSSVVYGVLWVLVRTVAWVCFRFQVVGQVPKTGGFIIAANHASY
                     LDIPLLGCGMRRRAWYLGRSDLFPARAVNRLLRWLGWIPLRLGRLDRDAFGKAVSLVK
                     EGKVVVIFPEGSRTENGQLQPAKAGIGMIVAQTGCPVIPAYLKGTHEVLPVRAKWPRF
                     RSVTVIYGEPIAFVPSRENGEGKKFYQEVSQTVMDRIAALGGVEPPIRHDTAGTRSAE
                     "
BASE COUNT         1756 a         2131 c         2464 g         1714 t
ORIGIN      
        1 caggaacgga tgaaagagtc ccttcgtatt tgtatcgaag cggcgaaaca acgacaggaa
       61 gcactcgatc acgcgatttt ctacggcccg cccggtctgg gcaagacgac tatcgctcac
      121 atcattgcca gggaaatggg cgcttcgcta cggtcgacct ccggtctcgt cttagcccat
      181 gccggtgatc tcgccgccat cctgatgaac ctccaagagc acgatgttct cttcatcgac
      241 gagattcacc gccttcccgc gtccgtcgag gaggcgctgt atccggcgat ggaggacttt
      301 cagctcgatc tggtcgtcgg gcaaggaccg gctgcgcgga ccgtcaagct cgacttgcca
      361 cgctttacct tggtgggagc gaccacgaga gccggggccc tgacgtctcc tctccgagat
      421 cgctttggac tcgttcatcg gttggaattt tactcccctg aagagctctg taccattgtg
      481 actcgctccg ccggtctact cggaattccc attgatcgcg acggggcagc ggaaattgct
      541 cgtcgcgcca gggggacacc tcggatcgtc aatcgattga tcaaacgcgt tcgagactat
      601 gcgcaagtaa aagcggaggg gcgcattacc aacatggtgg ctcaagaagc gctcgtatgg
      661 ctcggagtgg atacagctgg attcgatgag atggatcgta gaattctcgt cacgattatt
      721 gaaaagttcg gtggagggcc ggtcggggtc gagtctcttg ctgcagcggt tcaggaggat
      781 aaaggaaccc tggaagatgt ctatgaaccc tacttgatcc aagcagggtt tctcgaacgg
      841 accggccgtg gacggcaagt taccaagctg gcgcatgacc acttcaaagt ttcgaccccg
      901 tttcttttct cataaggaat ccagagcaga cagctcgaaa tgcttccgtg gaaggtgatg
      961 aaccgatcga ccacggtcgc gcaagttcct gaaacactga ggatctcctt gcattcccgg
     1021 ggatcgttct tgtatcattt cttgttcttc ctacacctgt ctaccctctg gacgctcgtc
     1081 caactcatga ggtgcgggtg gaaggggtcc gcgcatggcc ggagaccttt ctgactaccg
     1141 gaaagaaatc gatcggatcg atgacgagat tcttcgtctt ctcaacgagc ggtcgaagag
     1201 tgtcatcgag attggccggc tgaagaagca gaaggacgcc gacgccaacc tccatactcc
     1261 tgctagggaa gccgcaatca ttgatcggct cacgcaccag agccagggac cattcccaac
     1321 cgaggcgatc cgctcggtgt accgggaaat catgtccgct tctctgtcgc ttgagggtcc
     1381 gcagaaggtc gcctacctcg ggccgagggc cacattcacg catatggcct gcatgcaaaa
     1441 gtttggttct tcggcccagt atgttcccgt gaacagcatc aaagacgtct ttagcgaagt
     1501 ggagcggggg cgagctcatt tcggtgtcgt cccgatcgaa aatacgacgg aaggcgtggt
     1561 caatcacaca ctggacatgt tcatcgattc gaatctgttg atctacggtg aggtgctgca
     1621 agaggtgtct catcatctat tgtcaaagtc aggggtagcc gacgacgtga agaagatcta
     1681 ttcgcatccc catgccattg cccaatgtcg caattggctg gagacgaatc tcccccatgt
     1741 tccggtctct gaggtggcga gcaccgcaag ggccgcggag atctgtgtgg acgacccctc
     1801 agcgggggcg attgcgtcgg agttggcggc acagctctac gggttgaagg tcatcaagag
     1861 tcggattgaa gataacatga acaactttac gcggtttctt gtcctctcgc aaaaacctcc
     1921 cgagcgaacc ggaaaggaca agacatcact gatgctgtcc gtgaaggaca aggtcggcgc
     1981 cctgtatgac ttgctccgtc cattcgcctc tcacgggttg aacatgacga agatcgagtc
     2041 gcgtccctcc cgtcgaaagg cctgggaata cattttcttc gtggatctcg aagggcacat
     2101 ggaagaagaa cgcgtcaaga aagcaattga ggaaatcaag agtcgctgcc tcttcatgaa
     2161 ggtgctcggg tcgtatccgt cctacaacta acgtcatgac aatcagagtt cacccagaca
     2221 tcgcctcgct cagcccctac gtgcccggga aacccattga ggaattgcaa cgggaactgg
     2281 gtctgacacg cgtgatcaag ttggcctcca acgaaaatcc cttgggtccc tcccccaaag
     2341 cactctccgt actgtcagaa agcgccagta cgcttcatcg ctatcctgat ggtggagcgt
     2401 accgtcttcg cgaggcgctg gcagatcggt ggaaagtgac gcccgatcac gtcattctcg
     2461 gaaatggttc agacgaaatt ctcggtctgc tcgcacggac cttcttgtcc ccgggggacg
     2521 aagctgtgat ggcggaccag acctttgtca tttacaagat ggaagtgacg gccgctcacg
     2581 gaaagccggc cgtgattccc ctgaaacagt ggcgtcacga tctgacggcg atggcagatg
     2641 cgatcaccga ccggacgaga cttctgttcg tgtgtaaccc caacaatccc accggaacaa
     2701 tggtgaccgg tacggacgtg gcccggctca tggagcgagt tccgtctcat gtcatcgttg
     2761 tctttgacga agcctactat gagtatgtcc ggagcccaca attcccggag tcgatggcct
     2821 atgtcaagca gggtcggaat gtgattgtgc tgcgcacgtt ttcgaagatt tatggccttg
     2881 ctggactacg gattggctac ggaatcacaa cgcccgagat cacgaatttc ttgaatcgcg
     2941 tgcgacctcc gttcaatgcg aattctctgg cccagcgggc ggcattggct gccttgggtg
     3001 acgacgagca cgtggcaaga agccggaccg tgaatggcgc ggggatggga cagatgaccg
     3061 ctggtctgaa agcgctcgga ttcgaggcgg taccgagcga agcgaacttc ctgtatttcg
     3121 acgtgggtcg agagggacgt ggtgtctttg agggattgct acgagaaggc gtcatcgttc
     3181 gccatatcga aggccccatg ctgcgggtta cgatcggcct agaagaagaa aaccaggctt
     3241 tcttggaggc gttgaagcgc gtactcaaga agtgaagaga ggcatgaggg gaccatgatc
     3301 atcgtcttga agccagaagc aacggaacga gaagtcgatc acatcatcga tcggttgcgc
     3361 gaattgggcc tgaaatcgca actctccacc ggccaggagc ggaccatcat cggagtcatc
     3421 ggcgatgatc ggatattgca gaatcagccg ttgaccgcgt tgccgggagt cgaaagcgtg
     3481 ctgcccatcc tcgcgccctg gaagctggtc agccgcgagt tcaagcggga ggcaaccatc
     3541 atcgatgtcg gaggcgtgaa gatcgggggc aagaaactgg cagtcatggc tggcccttgc
     3601 gccgtggaac ggctcgaatt gacggtcggg atcgcccatg acgtcaaagc ggccggagcg
     3661 actatcttgc gcggcggagc gtacaaacct cgcacgtcgc cctattcatt ccaggggctg
     3721 gggcgcgagg gcctcgacta cttgatcgaa gcaaagaagc aaaccggatt gccggtggtg
     3781 agtgaaattc tcgacacgcg cgacatcgaa ctatttttgg aaaaggccga catcattcag
     3841 atcggcgcgc ggaacatgca aaacttcgaa ttgttaaagg aggttggggc ctacgacaag
     3901 cctgtactcc tcaagcgagg attatccgcc acgattaagg agtttctcct gtcggcggaa
     3961 tacatcatgt cgcgtggaaa tcggaacgtg atgttgtgcg aacgagggat cagaacgttt
     4021 gagactcaat accgcaatac gctcgacttg gcggcgattc ctacgttgaa agatctgtca
     4081 catttgccgg tcatcgttga tccgagccac gcgaccggtc gatgggatct cgtcgctccg
     4141 atgtcgaagg cggcggtggc cgccggggca gatggcttgc tgatcgaggt tcattcgaat
     4201 ccggagtgtg ccctctgcga tggtgaagaa tccatcaggc caaacaaatt caaagaactc
     4261 atgcaggaac tgcggaagat tggggaggcg gtgggacgag aggtgtaggc cgccaccccg
     4321 gctttcgacc ccggtcttgc ccttccatac agcacattcc atgactgtcc atttcaccca
     4381 ggtcaccatt gtcggcgtcg gtctcatcgg cggctcgctc gggatgattc tcaagcgaaa
     4441 agggttggcc gatcgggtcg ttggtgtcgg gcgtcgagtc gaaaacctga agaaagcggt
     4501 cgagctgggc gccatcgatc gctacgtcgt ggactcgaca gagggggtgc gagaggccga
     4561 tctcgtcgtg ctggccacgc cggttgatac ctatgagcgt catctgaagg aatgggcttc
     4621 ctgcctcaag accggtgcca tcgtgacgga cgtcggaagc gtgaagggtc cattggtcaa
     4681 gcaggctgag gcggcgctgc ctcacggtgt ccattttgtc ggagcccacc cgatcgccgg
     4741 gaaggaaaaa accggggtgg tcgcggggac tccccagctg tttacgggcg ctcggtgcat
     4801 catcacgccg acgaaaacaa cggatccgga ggcgttggaa cgcgtgcggg ccttgtggcg
     4861 ggagacgggc tctgtggtgc tggaaatgga tccatatgag cacgacaaga ttctcggagc
     4921 agtcagccat ctgccccacg tcgcggcctt cgcattaacc aatgcgctca tcgatctgcg
     4981 agagcgtctg attccctcgc tggacctggc aggacactcg ggcggcgggt tgcgcgatac
     5041 caccaggatt gcagccagct cgccggaaat gtggcgggac atttttcttt ggaatcgcga
     5101 caacatcgtc gcgtgtatcg aagcctacga acggtcgctg gctcggctga aacagttgat
     5161 caaatccggc gatgccgccg gaatcgaaaa agaactggaa cgcgcgaaac aggagcggga
     5221 gaagttccca gtccagcctc cctcgaacac gtaggaagct atggcgtcgt taacgattac
     5281 accagggcgc tcgctgaacg ggacgatctc ggtcccaggt gataaatcga tcactcatcg
     5341 ggccatcatc ctgacggcat tggccgacgg cgtcagcacg gtgtcgaaat attgccgtgg
     5401 cgaggattgt ctcaacacga tacgggcctg tcaggtgctt ggcgtgcgaa ttgaggtgtc
     5461 cccggagacg ttgcgggttc acgggaaggg tctttggggc ttgagtgaac cggcagctcc
     5521 gatcgattgc ggcaattccg gcacgggaat ccgacttctg actggtcttt tggccggaca
     5581 ggacttcttt accgtcctca caggtgatga atcgatccgt cgtcgtccga tgggtcgcgt
     5641 cgtcaagcct ctccgtgaaa tgggcgccat gattgcggga cgtaaaggtg gcgagctggc
     5701 tcccttggcg gtcacaggaa gtaaacttcg ggcgatcgcg tatgcttcgc ctgtcgccag
     5761 tgcccagatc aagtcgtctc tcttactggc ggcattattc gcccaaggca caactcggat
     5821 cacggaacct cgcctctccc gggaccatac ggaacgcatg tttcagtact tcgggattcc
     5881 actttgtcgc gaagggctca cagtatcgct tgatggacgt cccacagtcg gttggaatgc
     5941 cgttcccgag ttgatcgttc caggtgacct gtcggcggcg gcattctttc tcgtcggagc
     6001 gagcattgtg cctggatcgg atatcatgat cgaaggggtt ggggttaatt ctacaagaac
     6061 gggcctgctc gaagtcctgg caagaatggg cgcggacatt caactcctga atcgtcgcga
     6121 aaaggcggga gaaccggtcg cagatctccg ggtgaagtcg gcagcactcc ggggggtgac
     6181 cattggatcc gacctcattc cccagacgat cgacgaattt cccattctct gtgtggctgc
     6241 cgccgtcgcc gagggtcaga cggtgatctc cggagcagaa gagctccggg tcaaagaaag
     6301 cgatcgcatt gccacgatgg cggcagaatt gagaaagctg ggcgtgcagg tcacggagcg
     6361 gccagatggg atgacgatcc aaggggtagg aaaggcgaac gaaaacggtc ggttgaccgc
     6421 ggccacctgc cagagtcacg gggatcaccg tgtcgccatg tctctggcca ttgctggcct
     6481 gaccgcctcc acgaacacag tgattgacga caccgcctgc attgagacat cattcccgaa
     6541 ctttcaatcg aagctattgg aattattgac taaagatgct tgaaggcgaa taatgtggat
     6601 ttagctctcg cgggggtgag gaagctaaag tgaaaggcgg aaaactcaac cagatgatta
     6661 ttgcgatcga tggtccggct ggcgttggca agagcaccgt tgcaaaattg ttagcggctc
     6721 gacttgggta tctctatttg gatacgggag cgctgtatcg agcggttgcc tggaatgtcc
     6781 ttcatcatag ggtggacccg gccgagtccc aggcggtcga gtcgctgctt tctggaacat
     6841 cgattcagat gctgttccag aatggatcgg ccgaggtctt ggtgaacgga aagaacatta
     6901 cacatgaact tcgcaccccg gctgtctcgg ccgctgcctc gatcgtttcc gctattccag
     6961 cggtgcgggc ctggttgctt cctgttcagc gtcaaattgg gagcgctggt gcggtggttg
     7021 ctgaagggcg cgatatcggg acaaaagtat tccctcaggc agacgtgaaa tttttcctcg
     7081 aggccgacgc cgatgttcga acacagcgac ggcaccgaga attggtcgcg gccgggcata
     7141 ccggggctct cgaacagacc aacgccgact tgattgggcg tgacaccaga gatcggacgc
     7201 gggcgatcgc gcctctcgtg gcggctgagg atgcgctgca cattgatacg tcagccttga
     7261 cggttgaggc tgtcgtcgat catatgatgg ttgtgatcac ggccaaattg tgagttccgt
     7321 ggtctatggg gtgctctggg ttctggtccg gacagtcgca tgggtatgtt ttcgatttca
     7381 ggtcgtcggg caagtgccga agaccggcgg attcatcatc gcggcaaatc acgctagtta
     7441 tctcgatatc ccgctcttag gatgcgggat gagacggcgg gcgtggtatt tgggacgaag
     7501 tgatttgttc ccagcccggg cagtgaatcg cctgttgcga tggctgggat ggattccact
     7561 gcgacttggg cgcttggatc gagatgcatt cgggaaggcc gtcagtttgg taaaggaagg
     7621 caaggttgtc gtcatttttc cagaaggcag tcgaactgag aacgggcaat tgcaaccggc
     7681 taaagcaggg atcgggatga ttgtggcgca aacagggtgc ccggtcatcc ccgcgtactt
     7741 gaagggaacc catgaggtac tgccggttcg ggcaaaatgg ccgcggtttc gttcggtgac
     7801 agtgatatac ggagagccaa ttgcatttgt cccttcgcgg gagaacggcg aggggaaaaa
     7861 gttttatcag gaggtcagcc agacagtgat ggatcgaatc gccgcgctcg gaggagtcga
     7921 gccgccgatc cgtcacgaca cagccggaac ccgcagcgct gagtgagttc cggttttatt
     7981 actatcagca cccgacttgt tcggaagagt aggaagctcc catgagtacg gtttcgaaac
     8041 agagtgacca acagttggat cgtgc
//
LOCUS       DATDNW010000192         6168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_92883,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000192 DATDNW010000000
VERSION     DATDNW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92883"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VI359_04765"
     CDS             complement(<1..310)
                     /locus_tag="VI359_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973595.1"
                     /translation="MMRWLRCGHAGTFPIGLLVVTGVLWSASEAVAGAITIAGNGPEL
                     RVIEQLTRAFEKNRFGTVVEIRWDPSFHPINMVKSGEADFAVAGQTDLDLVAVPIAWD
                     G"
     gene            complement(675..968)
                     /locus_tag="VI359_04770"
     CDS             complement(675..968)
                     /locus_tag="VI359_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973596.1"
                     /translation="MRIHLFIKRAVLCLSLLLIASSPTLSTQAEDQLVTGAMVFESAK
                     DLGGVAAGAVEDTLKACLARIPLDASVGQRLLAEQSCAGEEGTRKLIPDAPKF"
     gene            1501..1902
                     /locus_tag="VI359_04775"
     CDS             1501..1902
                     /locus_tag="VI359_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973597.1"
                     /translation="MLMPLFLLLILIGFPLSASAEFINKGDRVEVGKGTRLCTNVKMD
                     KAKGSLDPGRVFWKVTSKMRSNGTQTVAAFVYEEIYDSKSGDTRVKETLYQEDYATFF
                     KRDPEHKGMLLMISPQDGRVEAIVEVCEKKK"
     gene            2160..3014
                     /locus_tag="VI359_04780"
     CDS             2160..3014
                     /locus_tag="VI359_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973598.1"
                     /translation="MMFHLNTAVGHRGACTAVAVIIAITGFVCSPADAVPIAVRFPEG
                     IAHGFLLVRSLAGEIIGQGEMTQVVKEGDLVDSHLVFTFKDGSLHDEKVTFSQQRVFS
                     MLRYHLVQRGPSFPNQIEVSIDRSTAEYKVRSKVGEDGKEEVLTGAFTLPKDVYNGIF
                     VTMLLNLPKGASETVNFLAFTPAPEVIKLELHLMGEHTVHIGDLSRKALQYVFKPDIG
                     MIRELLGKAAGKLPAHFHYDCWVLADEVPSFVQFEGPLQLMGPIVRIELVSPRLSTKP
                     EDKKVFPK"
     gene            3398..3565
                     /locus_tag="VI359_04785"
     CDS             3398..3565
                     /locus_tag="VI359_04785"
                     /inference="COORDINATES: protein motif:HMM:NF018715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY6973599.1"
                     /translation="MLYYALVFLVVGLIAGALNLAGVSAVAVQISWILFLIGIVLVAI
                     HVITGRTARVA"
     gene            3661..3888
                     /locus_tag="VI359_04790"
     CDS             3661..3888
                     /locus_tag="VI359_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY6973600.1"
                     /translation="MNADQLKGKWAQFKGELKQKWGKFTDDDLQQIEGNYEKFIGKVQ
                     ERYGDKKSELMKWADQWYHKSQPTATEKKTS"
     gene            3952..4509
                     /locus_tag="VI359_04795"
     CDS             3952..4509
                     /locus_tag="VI359_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY6973601.1"
                     /translation="MTTRYVLTLCTALVIGGALITGSANAVGKADEKTPMNDSYLTAK
                     TKIALFADSRVKGSEINVETTQGAVMLRGKVDSDTAKQAAEDITKGLDGVKTVKNELQ
                     VVAPSKREAIDDKDEAITARVNEQIAKDSQLKKAGIRAQTNAGVVSLTGEVPDLMTSA
                     QASWYAWQVPGVKSVKNDLTVKEKA"
     gene            4610..4852
                     /locus_tag="VI359_04800"
     CDS             4610..4852
                     /locus_tag="VI359_04800"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY6973602.1"
                     /translation="MSRVTISSPATPTLSASTPKVSPTTEQSGPAIQISKADWTHERI
                     ERRAYELYEQRGRQEGRALEDWVKAERQLVGASGKS"
     gene            4908..5381
                     /locus_tag="VI359_04805"
     CDS             4908..5381
                     /locus_tag="VI359_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY6973603.1"
                     /translation="MSTIVRWDPIRTRWNPFNELQELESRWPSMFPLRAATGNGGKEA
                     ITVAEWSPLVDIMEDEKEYLIKVELPDMKKEDVKLTVENDVLTISGERKYAKEERGEK
                     HHRIERAYGSFLRSFSLPEDADGSKVSADYKDGMLKVHLPKSEKAKPKAIEIKVA"
     gene            5859..>6168
                     /locus_tag="VI359_04810"
     CDS             5859..>6168
                     /locus_tag="VI359_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973604.1"
                     /translation="MMRWLRCGHAGTFPIGLLVVTGVLWSTTEAVAGAITIAGNGPEL
                     RVVEQLTRAFEKNHFGTVVEIRWDPSFHPINMVKSGEADFAVAGQTDLDLVAVPIAWD
                     G"
BASE COUNT         1430 a         1614 c         1812 g         1312 t
ORIGIN      
        1 ttccatccca ggcaatggga acggcgacga ggtctagatc tgtttgtccc gccactgcaa
       61 aatccgcctc gccagacttg accatattga ttggatggaa cgaagggtcc caacggatct
      121 caacgaccgt tccgaaacgg ttcttttcaa aggctcgggt cagttgttcg ataacgcgaa
      181 gttcaggacc attgccggca atcgtgatcg cgccggccac ggcctcggat gctgaccaca
      241 ggacacccgt taccaccaac agcccgattg ggaaagtccc cgcatgacca cacctgagcc
      301 atctcatcat gtgcgtcctc tcatttccta atctttctgg tccttccctc cgcactcgct
      361 attcctcaat gaagggaccc tcacaatcgg atcgaagatc ggcagaatca tgccccccac
      421 tagaaggccc aggggccaga ctgcgcagag cgagctctgg cttatccttt cacttcaacg
      481 ttgcttgccc agatggccgc tccccatgta aaaaacatca ggctcaataa ggctataatc
      541 tgatacatct cgctcacctc tctttcaatg aatcttcgag ttcacttcac tcttctatgt
      601 ctatgccgat cgattctcag aagctgcggc tttgcagggc ctcagctcct gttcgggcgt
      661 gttatgagcg tcgttcaaaa cttcggagcg tctgggatca gttttcttgt gccctcttcg
      721 cctgcgcagc tttgttcagc caacaggcgc tgcccgacac tcgcgtccaa tggaattctg
      781 gctaggcagg ccttcagcgt gtcctccact gcccccgctg ccactcctcc gaggtctttc
      841 gccgactcga acaccatagc tcctgtcaca agttgatcct ccgcctgagt tgacagcgtc
      901 ggcgatgacg caatcagcaa aagcgacaaa cacaggactg cccgcttgat gaacagatgg
      961 attctcatgg catacccctc cttgaatcac gtaggcccgg gattgacgct tcagagcggg
     1021 cccactcgat gaagcgcgcg tgaaccatcc ggtcgaatca ctcttctgct ctgtctcatg
     1081 gatcagcaga aatccttcgc tatccccatg cagccacggg aggcacgaat acgagtacca
     1141 tgaggcatgg catttacaca caagaccgat tgactcgatg agacgctttg ctcgagggag
     1201 cggcggaaac cagggaatca gtcgcgcagg aaggaaagag gaagcgcgag ggatgggaca
     1261 gtcgtcgtgg ccctctgcgt ctaggatcac gctaagcgca atgggcaccg gagtcaagca
     1321 ctataaatgg gggcatggat agttcaatga ttctgcgggt cctcagaggt cccacacgtt
     1381 ccaccatcgg agattcgatg atcccctggc cgtgcgctcg attttctaat ggaatcgcgg
     1441 tatccttccc gcgggtcagc agcacatgtc tttaaactca atttcaaaga ggattgtcac
     1501 atgttaatgc ccctcttttt gcttctgatc ctcatcggat ttcccctgtc agcctcagct
     1561 gaatttatta ataaagggga tagggttgag gtggggaaag gaacgagatt atgtactaac
     1621 gtgaaaatgg ataaggcgaa ggggtctctg gatccaggtc gcgtgttctg gaaggtaacg
     1681 agcaaaatgc gatcaaatgg tactcagact gtcgccgctt ttgtctatga agaaatttat
     1741 gacagcaaga gtggcgatac gagggtcaag gagaccttat accaagagga ttatgccacg
     1801 ttctttaaaa gagacccaga gcataaggga atgcttctca tgatcagtcc gcaagatggc
     1861 cgagtcgaag ctatcgttga ggtgtgtgag aaaaagaagt aggggaggtc ttgcagtcgt
     1921 gcatgggctc tcgccaagca ggtgatcgaa gaagcaacgg ggacgcctat gaaaaagacc
     1981 gtatgctgat ctctgaattc caagtgaccg agcgcgactt ggctgacccg gcagcgttcg
     2041 tcggggcgct cgatcaatcg atcgagcacg tcgaagagga agaaccctga gggcacggcg
     2101 ccgatcctcg gttccgacga gaatatcatg gtgtcagaca cagtttctgc ttaccccgca
     2161 tgatgtttca tctgaacacc gctgttggcc atcgtggtgc ctgcacagcc gtcgcggtca
     2221 tcattgcgat cacgggtttc gtgtgctcgc ctgcagacgc cgtgcctatt gcggtgcggt
     2281 ttccggaggg cattgcccac ggcttcctgc tcgtgcggtc gcttgccggg gagatcattg
     2341 gccagggtga gatgactcag gtcgtcaaag agggagatct ggtcgacagc catttggtct
     2401 tcactttcaa ggacggctct ctgcacgatg agaaagtgac cttctcgcaa cagcgtgtgt
     2461 tttcgatgct ccgctatcac ctggtccagc gtggcccgtc gtttccgaac cagatcgaag
     2521 tctcgatcga ccgaagcacc gcggaataca aggttcgctc caaagtgggg gaggatggga
     2581 aggaggaggt gctgaccgga gcgtttacgc tgcccaagga tgtgtacaac gggatctttg
     2641 ttacgatgtt gctgaacctg ccgaaaggtg ccagcgaaac ggtgaacttt ctcgccttca
     2701 cgccggcgcc agaagtcatc aagctcgagc tgcatcttat gggtgagcac accgtccaca
     2761 tcggtgacct gtcacgtaag gcgctgcagt atgtcttcaa gccggacatc ggaatgattc
     2821 gggaattgct cggcaaggcc gccggcaagc tcccggctca ttttcactat gactgttggg
     2881 ttctggccga tgaggtgccg agtttcgtgc aattcgaagg gcccttgcaa ctcatggggc
     2941 cgatcgtgcg aatcgaactt gtgagccctc gcttgtcgac aaagcccgaa gataagaagg
     3001 tgtttccaaa ataacgtcgg cgacacaatt agagaagcat tctgctattt gcgcaacctc
     3061 tttctaggga atggccaggc tgtcccttac tgaccgggtc gcaacgtacc tgtatcattg
     3121 aactatccat gcccccatta tagtgcttga cgctgctccg cattgcgcgt agcgtgactc
     3181 tgtgacgtat tgagctccgc tgactgttct atccctcgcg cttcctgttc cttcccacgc
     3241 gtgaccgatt ccccagtctc cgccgctttt gcgccagcgt atctctgatt cgctgcttgt
     3301 ctggtgtcag ggcctgctca tggaggcggc caggactccg gccatgctcc agaggactgt
     3361 tcaactcacg cccgcattcc atagagagga gtgagccatg ctctactacg cactggtgtt
     3421 tttggtggtt ggcctgattg ccggcgccct gaatctcgcc ggggtgtccg cggtcgcggt
     3481 ccagatttcc tggatcctgt tcctgatcgg gatcgtcctg gtcgcgatcc atgtgatcac
     3541 ggggcgcacc gctcgggttg cgtgactggg gctgtcgggt tgtccgaccg tatcccggcg
     3601 tgatgacctg ccgccaacgt gaagctgagg tatcgacact ttacacagga gggagatccg
     3661 atgaacgctg atcaactcaa ggggaaatgg gcgcagttca aaggcgaact gaaacagaag
     3721 tggggcaagt ttaccgatga tgacttgcag cagatcgaag ggaactacga gaagtttatc
     3781 gggaaagtcc aggaacggta tggcgataag aagagtgagc tgatgaagtg ggcggaccag
     3841 tggtatcaca agtctcagcc gaccgcgacg gagaaaaaga cctcgtgacg ggaatcgatc
     3901 aatgagacac gtctcactat acaatttcac atctcggtta aggagaagat catgacaaca
     3961 cgctatgtac tgacactctg cacggcgctg gttatcggcg gggcgctcat caccggctcg
     4021 gcgaatgcgg tcggcaaggc cgatgagaag acgccgatga atgactcgta cctgacggcg
     4081 aagaccaaga ttgcgctgtt tgccgactcc cgggtcaaag ggagtgagat caacgtcgaa
     4141 accacacagg gcgcggtgat gctccgcggc aaggtcgatt ccgatacggc gaagcaggcg
     4201 gcggaggaca ttaccaaagg cctcgacggc gtcaagacgg tgaagaacga acttcaggtc
     4261 gtcgctccgt ccaagcgcga ggcgatcgac gacaaggatg aggcgattac ggctcgcgtc
     4321 aacgagcaga tcgcgaagga ctcgcaattg aagaaagctg gcattcgtgc gcagacgaac
     4381 gcgggagtgg tctcgctgac cggcgaggtg ccggacctga tgaccagtgc ccaagcgtcc
     4441 tggtatgcct ggcaggtccc tggcgtgaag tccgtgaaga acgatctgac ggtgaaggag
     4501 aaggcctaag gtttcactcg cacgacccat gcgctctccg tccgccgaga accggccaca
     4561 acggagtcgg cagacggcag cgcacagggt ttacatgagg aggtgcacga tgagcagggt
     4621 aaccatttca agtccagcga cgccgacatt gtcggccagc acgcccaagg ttagtccaac
     4681 cactgaacag tccggtcccg ctatccagat atcgaaggcc gattggactc acgagcgcat
     4741 tgagcggcga gcctatgagc tatatgagca acggggtcgg caggaaggcc gggccttgga
     4801 ggactgggtg aaggcggaac gacaactggt tggcgcttcg ggtaaatcgt gaaacgaacc
     4861 atgagggtat tgtgagttca cttctatgac taaaaggaaa tgagaccatg agcacaatcg
     4921 tacgatggga ccctattagg acacgatgga atccttttaa tgaactgcag gaattggaat
     4981 cgcggtggcc gtccatgttt ccgcttcggg cggcgacggg gaacggaggc aaggaagcca
     5041 ttacggtggc cgagtggtcg ccgctggtag acattatgga ggacgagaag gaatacctca
     5101 tcaaggtgga attgccggac atgaagaaag aggacgtcaa actcacggtg gagaacgatg
     5161 tcttgacgat ttccggagaa cggaaatacg cgaaggaaga aagaggggag aagcatcatc
     5221 ggatcgaacg cgcctacgga agcttcctgc ggagcttctc gcttcccgag gatgcggatg
     5281 ggagcaaagt gtctgctgac tacaaggatg ggatgctcaa agtgcatctc ccgaagtccg
     5341 agaaagcgaa accgaaagcc atcgagatca aggtggccta aggcccttgt gttgatccgg
     5401 ggatggggag cgtcagggaa ggcgtccgta cgccgacgcg atgatggagt atttctgacg
     5461 ctcctccgcg aggtggctcc caagttctga acgcctgtgg cgtcacatcc gagcggagtt
     5521 gcagttcaag aagagacagg ttcgaaggac acgatcatcg aagcacacgc cttctgtaca
     5581 cacagcaggt tgtgaagaca gtgaaagaga ggtgagcgag atgtatcaga ttatagcctt
     5641 attgagcctg atggttttta catggggagt ggccatctgg gccagcaacg ttgaagtgaa
     5701 acaataagcc agagctcgtt ctgcacagtc tggcccctgg gccttctagt ggggggcatg
     5761 agtctgtcga ccttcgatcc gattgtgaga gccgcttcat tgaggaatag cgagggcgga
     5821 gggaaggacc cgaaaggtta ggaaatgaga ggacgcacat gatgagatgg ctcaggtgtg
     5881 gtcacgcggg gactttccca atcgggctgt tggtggtaac gggtgtcctg tggtcaacaa
     5941 ccgaggccgt ggctggcgcg atcacaattg ccggcaatgg tcccgagctc cgggtcgtcg
     6001 aacaactgac tcgagccttt gaaaagaacc atttcggaac ggtcgttgag atccgttggg
     6061 acccttcgtt ccatccaatc aatatggtca agtctggcga ggcggatttt gcagtggcgg
     6121 gacaaacaga tctagacctc gtcgccgttc ccattgcctg ggatggaa
//
LOCUS       DATDNW010000193         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_445102, whole genome shotgun sequence.
ACCESSION   DATDNW010000193 DATDNW010000000
VERSION     DATDNW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445102"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1366)
                     /locus_tag="VI359_04815"
     CDS             complement(305..1366)
                     /locus_tag="VI359_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973605.1"
                     /translation="MRERRWETTSPLTFGQVLAVGERLAALGLKPAVPAKDIICYVEE
                     WTVDSPDDFDQLDPWGTEDVTLVHIREGWRGDFFLLAGAYHTTYQQYQDTGTYCSISH
                     PWRIREPLQLHDQRSMVWLGFRHAHSFVRVRLQTHEVIPPGETRGDAQRERWVEERRT
                     AFLEAITQLELPIETGVEKGSLVLRPADTSVPFFCSWPDAFGPCQFEYNTADAYEFLV
                     PASKLVATFAPEPAGVRAYLTGFSEDAFGEFQAIEPSARHAYRCSVHCPLDDLPEIRS
                     VIEPDGRLYATFCEFQTSELIPEGEDASAILGIVGDHAGFQIEARLNRAPLGEDLMAN
                     WLERVVGYPVVYAPLPAFV"
     gene            complement(1391..2041)
                     /locus_tag="VI359_04820"
     CDS             complement(1391..2041)
                     /locus_tag="VI359_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973606.1"
                     /translation="MRQIAGRYGLIIATLFSACLVTGCGGTGRTFYMDLQQKALLAQY
                     VEPEPVKIVVEPFDDRRLEKTRLGMRTHLWGGVTYFQVAGERPGYVVAQALVERLKTR
                     GWRDRAWNVSMAAPGSVTDADIVISGQILDLSANAKSRVFSTVLSSGLKLVVTARNVG
                     DRTTTMRSVEGAQYDTVVWFSDNDLQELTRATLQDGIERYIVDTAIDQRALRPSRQ"
     gene            complement(2215..>2587)
                     /locus_tag="VI359_04825"
     CDS             complement(2215..>2587)
                     /locus_tag="VI359_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973607.1"
                     /translation="FEGTDKFTFRTLFWWGHHLACAILLEGTGIKQYKKHIVDRFHRL
                     AGQELELSLAPTLWEWKRGEGYTLPMTHDRKARIAAVLAERPFLKIVRFVPLTDPRVQ
                     LGQLPNLSRETFRAVLPIVAR"
BASE COUNT          563 a          789 c          729 g          506 t
ORIGIN      
        1 gagtgagagt cagcgtgtca taggtgacgc ccgagagaaa aaacacagcg ggcatgaacg
       61 gtttggccag cagggattgg agcttcgcca ccgggctcat gtccccactg tgcgaaatcc
      121 ttctcgtttg ttcaatgaaa aataggattc agacgatctg aattagagac ccaatcgaac
      181 acagaatgtt caaacagtcc atccggcaag gccgcagcga gcgaaaacct gaggcgtacg
      241 tttcttgtac gttgaaggtt tgagcgatgc gagaacgcag ccggtgggct ttttcaacat
      301 tctgtcagac gaacgcgggc agcggcgcat agaccaccgg ataccccacc actcgttcga
      361 gccagttcgc catcaagtct tccccgaggg gagcccgatt gaggcgggcc tcgatttgaa
      421 atcctgcgtg gtctccgacg ataccgagga tggcagaggc gtcctctcct tcgggaatga
      481 gttccgacgt ctggaactcg cagaaggtgg cgtagagacg cccgtccggt tcgatgacag
      541 aacggatttc cggcagatca tcgagcgggc aatgcaccga acagcgatag gcatgccggg
      601 ctgacggttc aatcgcttga aactctccga acgcgtcttc ggaaaatccc gtgagatagg
      661 cccgcacgcc cgccggttcc ggtgcaaatg tcgccaccaa tttactggcc ggtaccagaa
      721 actcatacgc atcggccgtg ttgtactcga attggcaggg gccgaacgcg tcaggccagg
      781 aacagaagaa cgggacggac gtatcggccg gacggagtac aagactcccc ttctccacac
      841 ccgtctcgat cggcaactcc agctgcgtga tcgcttcgag gaacgcagtc cgccgttcct
      901 ccacccatcg ttcgcgttgc gcatctcccc gcgtttcacc cggaggaatg acctcgtgcg
      961 tctgtagacg gacgcgcaca aaggaatggg cgtggcggaa gccgagccag accatgctcc
     1021 gctgatcatg gagctgaagc ggctcacgaa tccgccaggg atggctgatg gaacaatagg
     1081 tgccggtgtc ttggtactgc tgatacgtgg tgtgataagc cccggcaagg agaaagaaat
     1141 cgccccgcca gccttcgcga atgtggacca gcgtcacatc ttcagtgccc cagggatcga
     1201 gttgatcgaa gtcatccggc gagtcgacgg tccattcctc gacataacag atgatgtctt
     1261 tggccggcac cgccggtttc aatcccaatg ctgccagccg ctcgcccacg gcgagcactt
     1321 gtccgaacgt cagtggcgaa gtggtttccc aacgacgttc acgcacagcc tgtgatccct
     1381 cgccccgata tcattgccga cttggccgca gtgcacgttg gtcgatggcg gtatccacaa
     1441 tgtaccgctc aatgccatcc tgcaaggttg ccctcgtcaa ttcctgaaga tcgttatcgg
     1501 aaaaccagac caccgtgtcg tattgcgctc cttcgacgct gcgcatcgtc gtagtccggt
     1561 ccccgacatt gcgggccgtg acgacgagct ttaacccgct cgagagcacc gtcgaaaaga
     1621 cgcggctctt cgcgttggct gacaaatcca atatctgccc gctgatcacg atgtcggcat
     1681 cggtcaccga tcccggcgca gccatgctga cattccaggc gcggtcgcgc catccgcgcg
     1741 ttttgagcct ctcgaccagc gcctgagcca caacgtaacc cggccgctcg cccgccacct
     1801 gaaaatacgt gacgccaccc catagatgcg ttcgcatgcc cagtcgggtt ttttcgagac
     1861 gacgatcatc gaacggttcc acaacgatct tgacgggctc cggctcgaca tactgcgcga
     1921 gtagcgcctt ttgctgcaaa tccatgtaaa acgtccggcc cgttccccca catccggtga
     1981 caagacatgc gctaaacaac gttgcgatga tgagcccata ccgtcctgcg atttgtctca
     2041 catgcgcctc cctgcgttag ctcgcctcct tcggcgaggc gggccaagtg tacccaagtt
     2101 aaagattaga gacaaatgtg aggcgaaagg cacgtggcaa gaggctaggg ggagagtcgg
     2161 attgatcgag atgaagttgt tacccattgc ctcttgcccc acaccacgta ccgcctatcg
     2221 cgcaacaatc ggcaggaccg ctcgaaaggt ctcgcggctg agattcggca gctgccccaa
     2281 ctgaacccgc gggtcggtga gcggcacgaa gcggacaatc ttgagaaagg gccgttcggc
     2341 cagtaccgca gcgatccgag ccttccggtc gtgagtcatc ggcagcgtat acccttctcc
     2401 gcgtttccat tcccagagtg ttggagcgag cgagagttcc agctcttgcc ccgcaaggcg
     2461 atggaaccga tccacgatgt gtttcttata ctgcttgatt ccagtcccct cgagcaaaat
     2521 ggcgcacgcc aggtggtggc cccaccagaa cagcgtccgg aacgtgaact tgtcggtccc
     2581 ctcgaag
//
LOCUS       DATDNW010000194         6841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_295241, whole genome shotgun sequence.
ACCESSION   DATDNW010000194 DATDNW010000000
VERSION     DATDNW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295241"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1421
                     /locus_tag="VI359_04830"
     CDS             177..1421
                     /locus_tag="VI359_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEY6973608.1"
                     /translation="MASHQETVILHGHIIDSLILAKVLDTILMMGGPFDLEEVQIGKT
                     REESSRARIVVRAGSAQLLNDILHRIQPHGASIEREKDCSLQPAPADGIFPEDFYPTT
                     HLPTQIRLRGEWVDVEGTEMDLGVSVDPKKAIVRTVPMGAVKRGELIVTGREGIRVIP
                     LQRPRDRDIFGFMESQVSAERPHRHVIHDVARRMSSMREGFRRGQLGSKVLMAGGPAI
                     IHAGGRDALTWMIEEGFIHVLFCGNALPAHDMEVELYGTSLGYGHQAGRVVPHGHEHH
                     LRTINRIRAIGSIKAAVESGVIKGGIMAACINKHVQVVMAGTIRDDGPLPGVITDSVM
                     AQDAMRAAIPGVGLALLVASTLHAVATGNLLPATIPSVCVDVNPAVPTKLADRGSFQA
                     VGLVMDAASFLRELARELGWTS"
     gene            1418..2218
                     /locus_tag="VI359_04835"
     CDS             1418..2218
                     /locus_tag="VI359_04835"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY6973609.1"
                     /translation="MSRLLVCPPDYFGIEYEINPWMRRSNTVNRDQAVRQWHGLMQVL
                     EQDVGAVLERMKPVPGLPDLVFTANAGIVVGRRAVPSRFRYVERQPEEARFEDWFRTH
                     GFEVLSLEKELYFEGAGDLLGFPDGWFGGYRQRSDIRAFPVLSEWFGREIIPLELVDS
                     RFYHLDTCFCPLSGGELLYFPTAFDRYGQAAIADRIAPHQRLAASEADALKFACNAVC
                     VGKHVILPDGCLGTERLLEERGYRPHPVPLSEFMKSGGSAKCLTLALD"
     gene            complement(2252..2602)
                     /locus_tag="VI359_04840"
     CDS             complement(2252..2602)
                     /locus_tag="VI359_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973610.1"
                     /translation="MKTCPSCRRQQTDLNRYCIGCGRQFESAPPTSPTRGDTTPEALN
                     LQILYGMVGVLILAVLFPPWETPPSQTPDFLGIHFILAPPTPDAVVSRLLLTIELVTI
                     AIAGLYASFLFRKQ"
     gene            complement(2704..3417)
                     /locus_tag="VI359_04845"
     CDS             complement(2704..3417)
                     /locus_tag="VI359_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6973611.1"
                     /translation="MKIVRVGTALLVMSVLPLFFFAQSTLSQSTAQAESYFAAQVGYA
                     FPQSLSNGKVTQDGFGGLDISDQPLKNSPMVGAKLGHYFTRVRWLGVETEVTYSNPHV
                     KQGSITFSGPGGPPVTSPILAGLTQRMIMWAPVTLLVRYPGQRLQPYAGVGPALFFAH
                     LNGPTAPPGQSGTAIGLNAEAGLRYYLTRHWSLSSEGKYNLARIGYTSNDSNPNADPF
                     GFRATYSVITVSLAIAYHF"
     gene            3725..4744
                     /locus_tag="VI359_04850"
     CDS             3725..4744
                     /locus_tag="VI359_04850"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY6973612.1"
                     /translation="MPAMSQIGVIGAGAWGTALAKHLAQKGHSIQLWAYEREVVDAIN
                     ATHENSRFLPGVTLPQTLRVTNSLAQATEGCESIVFAVPSHVARAVLQQLAPVLSDEV
                     AIISATKGVEEDTLKLITQVMEDVLPQSCHQSLMVLSGPSFATEVSRGQPTALCLAGK
                     NSTIVTRFQSVLMSSTLRVYADHDVVGVQLGGALKNVMALAAGIIDGLGLGHNTRAAL
                     ITRGLAEMVRLGTAMGADPRTFYGLSGVGDLVLTCTGTLSRNHMVGVRLGQGERLEAI
                     LGGMHAVAEGVRTAKAAQGLAHRHRVEMPIVQEINAVLFEGKSCRRAVTDLMEREAKA
                     EKGAS"
     gene            4741..5502
                     /gene="larB"
                     /locus_tag="VI359_04855"
     CDS             4741..5502
                     /gene="larB"
                     /locus_tag="VI359_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY6973613.1"
                     /translation="MSPTKLRQLLAKVRSGTLSIEAALKHLRTLPYEDLGFASLDHHR
                     SLRQGFPEVIFCEGKTPAQIRGIARSLLNHHRPFLATRATREVATIIRRLDRRAAYHE
                     AARIVAIRDPKRTLRGHVLVITAGTSDIWVAEEARVTAEVMGSHVETLYDVGVAGIHR
                     LLERQHRLIEAKVAVVVAGMDGVLPSVVGGLVDYPVIAVPTSRGYGASFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINILGTGAKG"
     gene            complement(5549..6043)
                     /locus_tag="VI359_04860"
     CDS             complement(5549..6043)
                     /locus_tag="VI359_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973614.1"
                     /translation="MAWRGFWLLFCATVILWTTTEVQAKSKPRTPRPEPELKILELKI
                     APNPYTIASGTLEFSALVQLPKELGGATILELSSLVTSPSKTSLRFLTSRKPIEPHTT
                     MDGADPPRISLVLTWDGFDHKKQPAVPGIYAFEVRAKLLANGEKGPRTMMVSWPKRGT
                     LEVK"
     gene            6254..>6841
                     /locus_tag="VI359_04865"
     CDS             6254..>6841
                     /locus_tag="VI359_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEY6973615.1"
                     /translation="MGAHLHFDCFSGVSGDMVLGALVDLGVPFSQLVKGLTGLRLSGF
                     TLKKRHVRRGAVHATKVDVIVRKGLHEPLSLRRIHRILVASRLPDIVKEQSQSVFERL
                     AEAEGQAHRVAKAKVHFHEVSVLDSFVDVVGGVLGCHLLGVTRITASPVNVGSGTLHS
                     MHGILPVPGPAVAALAKGIPIYSAGPRRELATPTGL"
BASE COUNT         1420 a         1795 c         2150 g         1476 t
ORIGIN      
        1 cctggtgcct atgccacgag cgttctgcgg gagttcatga aaacacctga gatgtcagac
       61 agttcgttgc gctagagccc tcgcacactc tcgtcgagct atttccattc atctgcccct
      121 gtccccgcgg ctgggtggta gaatttcacc agactcgtca atcggaggtc ccctccatgg
      181 cgtcccacca agaaaccgtc attcttcacg gccatatcat cgattcgctg atcctagcga
      241 aggtcttgga cacgattctg atgatgggtg ggccgttcga tctcgaggaa gttcagatcg
      301 gtaagactcg cgaagaatcg tcccgggcca gaatcgtcgt gcgcgccgga tctgcgcaat
      361 tactcaatga catcctgcac cggattcagc cccacggggc ctcgatcgaa cgcgagaagg
      421 attgcagtct tcaaccggct ccggcagatg ggattttccc cgaggatttc tatccgacca
      481 cccatttgcc gactcagata cggctacgag gagaatgggt ggatgtcgag gggactgaga
      541 tggatcttgg tgtctctgtc gatccaaaga aggcgatcgt ccgtactgtg ccgatgggag
      601 cggtgaaacg gggtgaactg atcgtgacgg gccgtgaggg aatccgcgtg attccattac
      661 aaaggccacg tgatcgcgac atatttggct tcatggagtc gcaggtgtcg gccgagcgtc
      721 cgcaccgcca cgtcatccac gacgtcgcca gacgcatgag ctccatgcgc gaaggattcc
      781 gcagagggca gctggggtcc aaggtcttga tggctggagg gccggcgatt atccatgcgg
      841 gcggacgtga tgcactgaca tggatgattg aagaaggatt catccatgtc ctgttttgcg
      901 ggaatgcgct gcccgcgcat gacatggagg tcgaactgta cggcacctcg cttggatatg
      961 ggcaccaggc cggacgggtc gttccgcatg gacacgagca ccatctgagg actatcaacc
     1021 gaattcgagc gatcggcagt atcaaggctg ccgtggagtc gggcgtgatc aagggcggga
     1081 tcatggcggc ttgcatcaac aaacacgtcc aggtcgtcat ggccgggacg atccgcgatg
     1141 atggtccatt acccggcgtg attactgatt cagtcatggc ccaggatgcc atgcgggcgg
     1201 cgattcccgg ggtgggattg gccttactgg tggcctccac gcttcatgcc gtggcgaccg
     1261 gaaacctgtt gccggccacg attccctccg tctgtgtcga tgtgaatccg gctgtgccga
     1321 cgaaactggc ggatcgggga agcttccagg ccgtcggact ggtgatggac gccgcctcgt
     1381 ttctgcgcga gctggcgcgc gaattaggat ggacgtcatg agtcgtcttc tggtctgtcc
     1441 ccccgactat ttcggcatcg aatatgagat caatccctgg atgcgtcggt cgaataccgt
     1501 gaaccgggac caggctgttc gacagtggca tggcctgatg caggtgctcg aacaagacgt
     1561 gggcgcggtg ttggaacgta tgaaaccggt gcctgggttg cccgatctcg tcttcacggc
     1621 gaatgccggc atcgtggtcg gacgtcgagc cgtacccagt cgctttcgtt atgtcgaacg
     1681 gcaaccagaa gaggcgcgtt ttgaagactg gtttcgcacg cacgggttcg aggtattgtc
     1741 gttggaaaag gagttgtact tcgaaggagc cggcgatctg cttggctttc ccgacgggtg
     1801 gtttggcggg taccgtcaac gatcggatat ccgagccttc ccggtcctca gcgagtggtt
     1861 tggtcgagag atcattccac tcgaactcgt ggacagtcgg ttttatcatc tcgatacctg
     1921 cttttgtccg ttgagcgggg gcgaacttct ctacttcccc actgcctttg atcgatacgg
     1981 ccaggcggcg atcgccgacc ggattgcgcc gcatcaacga ctcgccgcgt ccgaagcgga
     2041 tgcgctcaaa ttcgcctgta acgcagtctg tgtcgggaaa cacgtgattc ttccagacgg
     2101 atgcctcggt accgaacggc ttcttgagga gcgaggctat cgacctcacc ccgtcccact
     2161 gagcgagttc atgaaatccg gtgggtccgc gaaatgcctc acgctggcgc ttgattgagc
     2221 tggcctagtt gggctgcaag ccacttgccg actattgttt tctgaacagg aacgacgcat
     2281 aaagaccggc gatggcgatc gtcacgagtt caatcgtcag gaggagacga ctcaccaccg
     2341 cgtcgggggt gggtggagcc agaatgaaat ggatgccaag aaaatccgga gtctgtgacg
     2401 gcggcgtttc ccacggagga aacagaaccg cgaggatgag cacccccacc attccgtaga
     2461 ggatctgaag attcagagcc tccggcgtgg tatcccctcg agtgggagag gtcggcggtg
     2521 cgctctcgaa ctgacgccca cagccgatac aatagcgatt gaggtctgtc tgctgccgcc
     2581 ggcaggacgg acaagtcttc atcaattgtt ctccagcgag gcaatagtcg acacggccct
     2641 gataggctac acggaccagc gtcggtttgc tagttggaat tgggatcgga gggaaaaacg
     2701 cgatcagaaa tgataggcga ttgccagcga gacagtgatg acactgtagg tggctcggaa
     2761 accgaagggg tcggcgttgg gattgctatc gttggaggtg taaccgattc gtgcgaggtt
     2821 gtatttgcct tccgaagaca gagaccaatg cctcgtcagg taataacgga gtcctgcctc
     2881 cgcattcaac ccgatggcgg ttcccgattg gcccgggggg gcagttggcc cattcaagtg
     2941 ggcgaagaac agcgcaggac ccacgccggc gtagggctga aggcgctgcc ccggataacg
     3001 gacgagcagc gtcacgggcg cccacataat catgcgctgg gtgagaccgg ctaagatagg
     3061 gctggtgacc ggaggtccac cgggaccgct aaatgtaatc gatccttgtt tgacatgcgg
     3121 gttcgaatag gtgacctctg tttcgactcc gagccaccga acgcgcgtga agtagtgtcc
     3181 gagttttgcg ccgaccatcg gagagttctt cagaggctgg tcagagatgt cgagtcctcc
     3241 gaaaccatct tgcgtgactt ttccgttact caaactctgc gggaacgcat aaccaacctg
     3301 cgcggcgaag tacgactccg cttgcgcggt tgactgcgag agggtacttt gcgcgaagaa
     3361 gaacaatgga aggacgctca ttacgagcag tgccgtgccg acgcgaacga tcttcatgcg
     3421 catcctccga attccatatc cgtacactcc catcctataa gccgacccat caagggcgaa
     3481 gggaaatgtg aaatccagac gttttggagc aagccgtgtg ccactccaga ttcgtctgtg
     3541 acgctatatg tgggattgct tatgatagac aacaacgtcg actgtaggct gcttggtgca
     3601 gtggtggaca agcctgttcg ttgacagggg gaaaccagct tcctataatc cgcccggcca
     3661 gcaccgcgct cggagaagcc acacggccca ctcaatacac gccacttagg gtattcttct
     3721 catcatgcca gctatgagtc agatcggagt gattggtgcc ggagcatggg gaacggcact
     3781 ggccaagcat ttggctcaga agggccactc cattcaactc tgggcctacg agcgagaagt
     3841 cgtcgatgcg atcaacgcca cgcatgaaaa ctctcggttc ttgccgggtg tgacgctccc
     3901 acagacgctg agagtcacca attccttggc acaggcaacg gaaggatgcg agagcatcgt
     3961 gttcgcagtt ccgtcgcatg tcgctcgggc ggttctccaa cagctggccc ccgtcctctc
     4021 cgacgaggtc gccatcatca gcgcgaccaa aggggtcgaa gaagatacgt tgaaactgat
     4081 cactcaagtc atggaagacg tgcttccgca gtcgtgtcac cagtcactga tggtgttgtc
     4141 cgggccaagc tttgcgacgg aagtcagtcg agggcaacca actgcgctgt gcctggcagg
     4201 gaagaacagc acgatcgtga ccagattcca gtcggtgttg atgtcctcga cgttgcgcgt
     4261 gtatgccgac catgatgtgg taggagtcca attgggcggc gcactgaaga acgtgatggc
     4321 gcttgcagct ggaatcatcg acgggcttgg gcttggtcac aacacgagag cggcgttgat
     4381 caccagagga ttggcggaaa tggtccgtct cggtacggcc atgggagccg atccacgcac
     4441 gttctacgga ttgtcggggg tcggcgatct ggtactgacc tgtacaggaa cgttgagccg
     4501 caatcatatg gttggtgtgc gattggggca aggggagcgg ttggaggcaa tccttggcgg
     4561 gatgcacgcg gtggccgaag gggtgcggac tgccaaggcg gcacaagggc ttgcacatcg
     4621 acatcgagtg gaaatgccga ttgtgcaaga aatcaacgcg gtgttgttcg agggaaaatc
     4681 ctgccgtcga gccgtaaccg acttgatgga gcgagaagcc aaggctgaaa agggggcgtc
     4741 gtgagtccga cgaagctccg gcagcttctc gccaaggttc ggagcggcac tctctcgatc
     4801 gaggctgcat tgaaacattt gcgcaccctg ccgtatgaag atttggggtt tgcctcgctc
     4861 gatcaccatc ggtcattgcg acaagggttc ccggaagtta ttttttgtga agggaaaaca
     4921 ccggcccaga ttcgcgggat tgcgcgatcc ctgttgaatc accatcgtcc gttcttggcc
     4981 acgcgcgcga cgagagaagt ggcgacgatt atcaggcgac tcgatcgccg cgccgcgtat
     5041 cacgaagccg cgcggattgt cgctattcgc gatccgaagc gaaccctacg cggtcatgtc
     5101 ctcgtcatca ccgccgggac gtctgatatc tgggttgcag aagaagcgag agttacagct
     5161 gaggtgatgg ggagtcatgt cgaaactctc tacgatgtgg gagtagcggg gattcatcgc
     5221 ttattggagc ggcagcaccg tctgattgaa gcaaaggtcg cagtcgttgt cgcaggaatg
     5281 gacggggtct tgccgagcgt cgttggaggc ttggtggact atccggtgat cgccgttccg
     5341 acgagccgag gctacggggc gagcttcggc gggttggcgg ctttgttaac gatgttgaat
     5401 tcctgtgcgg cgggtgttgg tgtgatgaac attgataacg gtttcggagc cgggtgtctg
     5461 gcgcatcgca ttaacatctt gggcacggga gctaaagggt aggggaagaa aattgtctct
     5521 tgtcactcgt ctcgcgtcgt tgaaccgctt atttcacttc taacgtgcct cgtttcggcc
     5581 aggagaccat catcgtccgt ggtcccttct ctccattcgc cagcagcttc gcccggactt
     5641 cgaatgcata gatgcctggt acagccggtt gtttcttgtg atcgaatcca tcccatgtca
     5701 acacgagaga gatgcgcgga ggatcggctc cgtccattgt ggtatggggc tctatcggtt
     5761 ttcggctggt gagaaaccga agcgatgttt tcgagggaga ggtgacgaga gacgaaagtt
     5821 caaggatggt cgcgccaccc aattccttgg gcaactggac cagcgccgaa aattccaacg
     5881 taccgctggc gattgtgtag ggattcggcg caattttgag ttcgaggatt ttcagctccg
     5941 gttccggacg aggagttcgc ggcttggatt ttgcctgcac ttcggttgtg gtccacagga
     6001 tgactgtggc acagaacagg agccagaatc cgcgccatgc caaggagcga gaacacggtc
     6061 ggtggaactg gaaggcgttg tgccaggtgt tcataacagg gatgagagag acatctccga
     6121 cagctgaggc cgctgagggg ataacacagt gccatgaggc tgtcaacgat cgtctcgcgc
     6181 agattgccgt tccgcagcgc cttgggtaag atggctccgc acgattccgg atgaacgacg
     6241 gaaaggatgg tcagtgggag cgcatcttca tttcgactgt ttctctggtg tcagtggaga
     6301 catggtactc ggggctctcg ttgacctcgg ggtgccgttt tctcagttgg tgaaaggatt
     6361 gaccggtctg cgcctgtcgg gattcaccct gaagaagcgg catgtacgtc ggggcgcggt
     6421 ccacgcgacg aaggtcgatg tcatcgttcg caaaggactc catgaaccgc tgtcattaag
     6481 gcgtatccat cgaattctcg tcgctagtcg cttgcctgat attgtcaagg agcaaagtca
     6541 atcagtcttc gaacgcctcg cagaagccga ggggcaggct caccgagtcg ccaaggccaa
     6601 ggtgcatttt cacgaggtga gcgtgttgga ctcgtttgtc gatgttgttg ggggagtatt
     6661 gggctgtcat ctcttgggtg tcacccgcat tacagcctct cctgtcaatg tgggaagtgg
     6721 aacgttacat tccatgcacg gaattctccc ggtgccgggg ccggcggtcg cagcattggc
     6781 gaagggtatt ccgatttaca gcgcggggcc gaggcgggaa ctggccacgc cgactggact
     6841 g
//
LOCUS       DATDNW010000195         4917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1116394, whole genome shotgun sequence.
ACCESSION   DATDNW010000195 DATDNW010000000
VERSION     DATDNW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116394"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VI359_04870"
     CDS             <1..181
                     /locus_tag="VI359_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973616.1"
                     /translation="YQDEGASFVPKYLDLLSTGGSEPPQTILAKVGVDMTSETFWQSG
                     FDTIRDMVQQLEHTL"
     gene            complement(744..1313)
                     /locus_tag="VI359_04875"
     CDS             complement(744..1313)
                     /locus_tag="VI359_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY6973617.1"
                     /translation="MAALAIIVQPAQADETQPAIKVLVAYHSLTGNTERMAEAVVEGA
                     KSVVGTDVVLKRVGQITADELFSADAIIVGSPVYWSNMSGEVKTFFDNWQFKFGVYPD
                     FKMKDKIGAAFATGGQVSSGKEVTMLTILAAMLGNQMIVVSGGGAFGASATTEGDSPG
                     IDNKEMADAQALGRRVAEVASIVKRGSVK"
     gene            complement(1428..1613)
                     /locus_tag="VI359_04880"
     CDS             complement(1428..1613)
                     /locus_tag="VI359_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973618.1"
                     /translation="MQEKRRVLYKKGVFVCPKCRQSYVQEKWIEEWRIRCHRCDYRGT
                     LTEVSVEEHMEEETIRR"
     gene            1750..2196
                     /locus_tag="VI359_04885"
     CDS             1750..2196
                     /locus_tag="VI359_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY6973619.1"
                     /translation="MLLFTLAFLTPSCTQAAGDSSTAGLIPITLPGGTLIHAEIADTA
                     RKRAEGLMYREHLAKNRGMLFTFDQAQPWTFWMKNTKIPLDIIWLNEKKQIVHIESNV
                     PICTRTDDGCRQYQPNEPALYVLELGGGMAEHYKLQKGTTLRFRVP"
     gene            complement(2204..4387)
                     /locus_tag="VI359_04890"
     CDS             complement(2204..4387)
                     /locus_tag="VI359_04890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY6973620.1"
                     /translation="MVYETVTDLDQLLDRVKSYHAEADLGVVRRAYEFSAKAHEGQRR
                     RSGEPYLQHLIAVAGVLTSLKTDVTAIVAGLLHDTLEDTLATPDELEKEFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEHLSKAKR
                     QEIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYETLRVRVAKRDEDRQQYI
                     QEVIQLVNKAMQENGLPGEVYGRPKHLFGIYQKMDKQSITFEEVYDLTALRIVTDTKM
                     NCYALLGVIHSLWRPVPGRFKDYIAIPKSNLYQSLHTTVVGPKGEHVEFQIRTEDMHR
                     VAEYGIAAHWKYKEQGRVGERDSKAFGWLHQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPKGIVKELPKGSTPVDFAYAIHTEVGDHCVGAKVNAKIVPLKHQLTSGDTVEIL
                     TSATQTPHKDWLKFVRTSRAKTKIKHWIKIEEQKRSIEIGRRLLESEFRRHGMAPAKM
                     LRSDELLDAARESGFETVDELTAAVGFGRVATAQVIEKLKGLETGEGKAVQERPAVPK
                     RITGKPDEKGVKVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTVDCPNLEAL
                     DYDRDRLVEVEWDASTPSTHAVKVSVMAVDKTGVLANVSSAIAECHANISRAEIATRE
                     DRKAVLDFIVEVSDTRHLEQVLKAVERVDGVITARRIRAWQERPS"
     gene            complement(4391..>4917)
                     /locus_tag="VI359_04895"
     CDS             complement(4391..>4917)
                     /locus_tag="VI359_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY6973621.1"
                     /translation="GGFDLYQGLSACRDLVDAFGGHPSAAGVTVRESRLDEFRARFCD
                     VVAEWASDAAKVPTLNVDAELRLDEVNLRLIQEIGVLHPFGVGNPEPTFAVTRLEVMD
                     ARTVGEKHLKMTVRQGASQPFDSIGFGMKSLLERGIPSRTPVDLAFTPELNRWDGYDR
                     IQLRIRDLRQSVIE"
BASE COUNT         1088 a         1428 c         1233 g         1168 t
ORIGIN      
        1 gtaccaggat gaaggtgcct ccttcgtacc caaatacttg gacctcctca gtactggcgg
       61 gtcggaaccc ccgcaaacaa tcctggccaa ggtgggagtc gatatgactt ctgagacgtt
      121 ctggcaatca ggatttgata ctattcgaga tatggtccag caactcgaac acacgctcta
      181 gaatcgcctc ggacagggtc agtatgccgg ccgattcctc atctccagcc tagggatctc
      241 ttgcagttac cccccttgac agcagaaacg ggtgaaggta tatttgtgtc agttgataac
      301 ggttatcata atcgtttgcg accaaacggc ttccgtatag agacccgact aagaaggagt
      361 acaacgaatg tatctctgtc tttgcaaggg aatcacagag tccgatgtca gggaagcggg
      421 acgtgcagga gttgtcatgc cctgccagct caaggcgcag ttcggcctga aggaagccgg
      481 ttgctgcggc cgttgctcaa agaacattca cgaatttgtg gaaatcgcga cgagtgaaca
      541 tcgtgcgccc tgtgcgaata ctgtgcgaag ctagcctcgc tcccagttgg tcgccaccac
      601 ctttcatttc attcgcacga tctctccccc atgggtccgg caataaccga cagaatacac
      661 ctctgcgagt tggagcctaa cagacgtagt gtgatgcctt gtggcggcac cgaggctgca
      721 agaagatgat aagagggaga tgactatttt accgaaccgc gcttcacgat gctcgcgact
      781 tctgctacac gccgaccgag cgcttgggca tcggccattt ccttattatc gatgcctgga
      841 ctgtctcctt ccgtcgtggc agatgcgccg aatgctccac cgccactgac gacgatcatc
      901 tgattaccca gcatcgcggc taagatcgtc aacatcgtga cttccttgcc actggatact
      961 tgcccaccgg ttgcaaaggc cgccccgatc ttgtctttca tcttaaaatc cggatagacg
     1021 ccgaatttga actgccagtt atcgaagaag gttttgactt ctcccgacat gttcgaccaa
     1081 tagaccggtg accccacgat gatcgcgtcg gcggaaaaga gctcgtccgc cgtgatctgt
     1141 ccgactcgct tcagcacgac gtcagtcccg accacgctct tggccccttc gacaacagct
     1201 tcggccatcc gttctgtatt gcctgttaga gagtggtaag ccacaagaac tttgatcgcc
     1261 ggctgagtct catcagcctg tgccggctgg acaatgattg ccaaggctgc catcagcagc
     1321 aaaatcgagt atccctctct tctgagattc ttccgattta tatgcattga agtaatgaag
     1381 acaacccgct cagccgtgga aggaaacact tgaagcattg aaacagtcta tcgtcgaatc
     1441 gtttcttcct ccatgtgttc ttcgaccgac acttccgtaa gagtcccgcg atagtcgcac
     1501 cggtgacaac gaatgcgcca ttcctcgatc catttttcct ggacgtatga ctgccggcac
     1561 ttcgggcaca cgaagacccc ctttttgtac aacacccgac gtttctcttg catggatatc
     1621 ctccctcaaa gtcagcggga ggatggcaca gcgcacgccg ggattgcaag ccgcactagg
     1681 ctgggttcag accttgactc tacattcaac gaccgatagg atttcgctgt gcagtcacga
     1741 tccctgagcg tgctcctctt cactcttgcc ttccttaccc cctcgtgcac acaagccgcc
     1801 ggcgactcat ctacggctgg cctgattccg atcacactac ctggaggcac gctcattcac
     1861 gctgaaattg ctgacactgc gagaaaacgt gcggaaggat taatgtatcg ggagcacctc
     1921 gccaaaaatc gaggcatgct gtttacgttc gaccaggccc aaccctggac cttttggatg
     1981 aagaatacga aaattcccct cgatatcatt tggttgaatg aaaaaaagca gatcgtccac
     2041 attgagtcaa acgtgccgat ctgcacgaga accgatgacg gttgtcgcca gtatcaacca
     2101 aacgaaccgg cgttatatgt cttggaactt ggtggaggga tggcggaaca ctacaagctt
     2161 cagaagggca ccacgctcag atttcgcgtg ccctagacac atctcatgac ggtcgctctt
     2221 gccaggcgcg aattcgtcgc gccgtaatga cgccatcgac acgctccacg gctttcaaca
     2281 cctgttcgag atggcgggtg tcggatactt ctacgataaa atccaagact gctttgcgat
     2341 cctctcgcgt ggcaatctcg gcccggctga tgttggcatg acattcggcg attgctgacg
     2401 aaacattggc caacactcct gttttgtcga ccgccatcac ggacactttc accgcatgag
     2461 tactcggcgt tgaggcatcc cactcgactt ccacaagtcg atcgcgatcg taatccaatg
     2521 cttccagatt cggacaatcg accgtatgga tggtgagccc gcggccacgc gtaatgtatc
     2581 ccaggatgcg atcgccagga accggattgc agcagcgaga cagttgcatc aacagatccc
     2641 gcgcgccctt gacctttacg cctttctcat cgggcttgcc ggtgatccgt ttgggaacgg
     2701 ccggccgttc ctgcactgcc ttcccttcgc ctgtttccag tcccttcagc ttttcaataa
     2761 cttgagccgt cgccacacga ccgaacccca ccgcggccgt caattcatcg actgtctcaa
     2821 atccagactc ccgggccgca tcgagcaact catcggatct cagcattttg gccggagcca
     2881 tcccatgacg gcggaattct gactccaaca gtcggcggcc gatctcgatg cttcgtttct
     2941 gctcctcgat tttgatccag tgtttgattt tggtcttggc ccgtgaggtg cgtacaaact
     3001 tcagccaatc cttgtgaggt gtttgggtag cggaggtgag gatctcgacc gtatcgccgc
     3061 tggtcaattg atgcttcagc gggactatct tggcgtttac cttggcgccg acgcaatgat
     3121 cgccaacctc cgtgtgaatc gcatatgcaa aatcgacggg agttgaccct ttcggtagtt
     3181 ctttcacaat acctttaggc gtaaacacat acaccacatc gtgaaagaga tcgagtttga
     3241 ctgaatccat gaactgccga ttgtccggca aatcttgatt ccattcgacg aactgatgaa
     3301 gccacccaaa ggccttgcta tcccgttccc cgacacgccc ctgctcttta tatttccaat
     3361 gcgcggcgat cccgtactct gcgacgcgat gcatgtcttc cgtgcggatt tggaactcca
     3421 catgctcacc cttcggcccg acgactgtcg tatgaagtga ttggtagaga ttggactttg
     3481 gaatcgcgat atagtcctta aagcgtcccg gaaccggtct ccacaaggag tggatcaccc
     3541 cgagcagagc gtagcaattc atcttcgtgt ccgtcacgat tcgcaaggcg gtcagatcgt
     3601 agacttcctc aaatgtgatc gattgcttat ccatcttctg atagatgcca aagagatgct
     3661 tcggcctgcc atagacttcg cccggcaatc cattctcctg catggccttg ttgacgagtt
     3721 ggatgacttc ttggatgtac tgttgccgat cttcgtctcg tttggcgact cgtacacgga
     3781 gggtttcgta tacgtccggc ttcagatgct tcaaacagag atcttcaagc tcgttcttaa
     3841 tccacccgat cccaagccgg tttgccaagg gcgcatagat ctctaaggtt tcttgcgcaa
     3901 tttcctgtcg cttggctttg cttaaatgct caagcgtacg catgttatgc agccggtctg
     3961 cgagcttgat gaggacgact cggatgtcgt ccgccattga gaggaccatt ttgcgaaaat
     4021 tttccgcctg cttttcttcg taattgcgga aggtaatctt gccgatcttg gtaaccccgt
     4081 cgacaagatg gacaacatct ttaccgaact ccttctcgag ttcgtccgga gtcgcgaggg
     4141 tatcttcgag cgtatcgtgg agaagcccgg ctacgatggc ggtgacatcc gtcttcagcg
     4201 atgtgaggac gccggccacg gcaatgagat gctgcaggta gggttcgcct gatcgccgac
     4261 gctggccttc gtgcgccttg gcggaaaatt cataggctct gcggaccaca ccgagatcgg
     4321 cctcggcatg ataacttttc actcgatcca gcagctggtc gaggtccgtg acggtttcgt
     4381 agaccataaa tcactctatc acgctctgcc gaagatcgcg gatacggagc tgaatccggt
     4441 catagccgtc ccaacgattg agttccggtg taaacgccaa atcgactggg gtgcgggacg
     4501 gtatgcctcg ttccaacagc gatttcatgc cgaagccaat gctgtcaaac ggttgcgacg
     4561 ctccctgtcg caccgtcatc ttcagatgct tctcaccgac ggtacgtgca tccatcactt
     4621 caagccgggt cacggcaaag gtcggctccg gattcccaac accgaacgga tggagaacgc
     4681 caatctcctg gatcaaccgc agattcactt catccaagcg tagttcggca tcgacgttga
     4741 gcgtgggaac tttggccgca tcgctcgccc actccgccac cacatcgcag aagcgtgcgc
     4801 gaaactcatc aagacgggac tcgcgcaccg ttacgccggc cgcgcttgga tggccgccaa
     4861 atgcatctac gagatcccga caagcggaca atccttggta gaggtcaaac ccgccga
//
LOCUS       DATDNW010000196         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_983247, whole genome shotgun sequence.
ACCESSION   DATDNW010000196 DATDNW010000000
VERSION     DATDNW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983247"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VI359_04900"
     CDS             complement(<1..327)
                     /locus_tag="VI359_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973622.1"
                     /translation="MPRPSIRFLTYRPIMAFRVFTLLVMALFTPVLSSPVLAEVRIVN
                     AQGEYRMGDRDTREDAIRLATEAAKRNALEQVSTYLESVTVVDGMDVTKDEIRTYTAG
                     LVLVLDQ"
     gene            510..1541
                     /locus_tag="VI359_04905"
     CDS             510..1541
                     /locus_tag="VI359_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY6973623.1"
                     /translation="MMVNIPTRSAQCGMVAILSVLFVFLCTSTSLNVALAVAPASHET
                     PTTSSTEYVILFVLEGFGQDSLKGGTMPTMSRLIKQGAVTWSATGVKPALRLPTMVSL
                     ITGMPVDKHGISWNTFDFSRGYPRAPSVFDYLDLSGGRDSAIFLMDESLYQIARPEPY
                     TDYQMCGPLKPECNPDRIVSYIRAYFKKAASGEGYGHAIQSLPHFLIVHLPEAGRVGV
                     AHGWGSKEYREALRTVDKAMASVLDIYKEQGLLKRTTVFVTSLSAEGKDAGGEQADGA
                     VPLLPWIASGIGIKHGHAIHQPVSILDTGATVMRTLGLKTHTEWESHAVEEIFQTASA
                     TAISPSAKQ"
     gene            1548..1988
                     /locus_tag="VI359_04910"
     CDS             1548..1988
                     /locus_tag="VI359_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973624.1"
                     /translation="MQRLVDKYAVQWVMVGAGLVLSAVMGASAAAGPPAAYLQEHLIT
                     IDATVLEPPTWWQVPGVTPSIYASDPESLDAYKTTEARALKLKPGKYKFVSFTFDFPF
                     GVNLDGKVEYAASLDQCVGGRGTQTLTVRCSRTYPHGGQRDYQY"
     gene            2004..>2869
                     /locus_tag="VI359_04915"
     CDS             2004..>2869
                     /locus_tag="VI359_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6973625.1"
                     /translation="MTHTLDDLLEALEDVDDATREEAAKTLAELGDPKSLDALVSACS
                     DDYWSVRAHAGVGAAKIGGPKALEAVIALFNDPIMEVRNQAVDAVVKMGSTVLDRMIA
                     VMRDDRWRVREHAAKTCGELKDRKAVEALIVACRDRDGAVKSAAAEALGKIGDPAAVP
                     ALIKLFRDSSKIVRETAGTALVYIGQASVDPLIETLKDKDFVVRCHAARALGGMTTDY
                     QIGRTWVREPRVVDALIAMLKDPDRAVREDATIALGQIGDPRAIDALIEAMKDGAVKR
                     HAIASLGMIGDAR"
BASE COUNT          600 a          783 c          892 g          594 t
ORIGIN      
        1 ttgatccagg acgagtacga gcccggctgt gtaggtgcgg atttcatctt tggtgacgtc
       61 cataccgtcc accaccgtga cgctttccag gtaggtggag acctgctcca gagcattccg
      121 cttcgctgct tcggtcgcca aacggatcgc gtcttctctc gtatcccggt cgcccatgcg
      181 atattcgcct tgagcattga cgattcggac ttccgcaagg acaggagacg agaggacggg
      241 ggtgaagaga gccatgacca gtagggtaaa gacccgaaat gccatgatgg gtcgataggt
      301 aaggaagcgt atcgagggac ggggcatcgc catcaccttc cgtgatagga ctgacgcacc
      361 agagaccctt tcgttgacct ccaaagacga gacagtctca gcatagcacc agcggtgtct
      421 tggcgccaga gcccaccgct gggcgtgagc tgccttgcct tggcggaaaa tggcgtgtta
      481 aggtacaaat tcttttctgc gggaaggaca tgatggtgaa tattccaaca cgatctgctc
      541 aatgcggaat ggtggctatc ctctcggtgt tgttcgtctt tttgtgcaca agtacctcac
      601 tcaacgtcgc tctcgccgtg gccccggctt cgcatgaaac ccccacaacg agttcgaccg
      661 aatatgtgat tctgtttgtg cttgaagggt tcggacaaga ttctctcaag ggcggcacga
      721 tgccgacgat gagccgcctg atcaaacaag gtgccgtgac atggtcagcc accggcgtca
      781 aaccggcgtt gcgactcccg acgatggtgt cgttgatcac cgggatgccg gtcgataagc
      841 acggaatttc ctggaacacg tttgacttca gccgaggcta cccacgggct cccagtgtgt
      901 ttgactacct cgacttgagc ggtggccgcg atagcgccat cttcttaatg gacgaatcct
      961 tgtatcagat cgcccgcccg gagccctaca ccgattatca aatgtgcgga cccctgaagc
     1021 cggagtgcaa tccggatcgg atcgtttcct acattcgggc ctatttcaag aaagccgcga
     1081 gcggcgaagg atatggccac gccatccagt cgttaccgca ttttctcatc gtgcatctgc
     1141 ctgaagcggg acgcgtcggc gtggcgcatg gatggggatc caaggaatac cgagaagcct
     1201 tacggactgt ggacaaggcg atggcttcgg tgctggacat ttacaaagag cagggcctgc
     1261 taaagcgcac aacagtgttc gtgacttcct tgagcgcgga aggcaaagat gccggcggcg
     1321 aacaggctga cggtgcggtc ccgctgcttc cctggattgc ctcgggtata gggatcaagc
     1381 acgggcatgc cattcaccaa ccggtttcga ttttggatac aggggccacg gtgatgcgga
     1441 cgttggggct gaagacccat acggagtggg agagtcatgc cgttgaagaa atttttcaaa
     1501 cggcctcggc gaccgcaatc tctccatccg cgaaacagta gtttgccatg caacgcctcg
     1561 ttgacaagta tgcagtgcaa tgggtcatgg tgggggctgg gctcgtcctg tctgcagtaa
     1621 tgggtgcctc ggcggctgcc ggtcccccgg cagcctatct tcaggagcat ctcattacta
     1681 tcgatgcgac cgtcctggag ccgcccacgt ggtggcaagt tcccggcgtc accccctcaa
     1741 tctacgcctc ggaccctgaa tcgttggatg cctacaagac gacggaagcc cgggccctca
     1801 agctcaagcc aggcaagtat aagttcgtga gttttacgtt tgattttcca ttcggggtga
     1861 atttggatgg aaaggtggag tacgccgcct ctctcgacca atgtgtcggt ggacgtggca
     1921 cacagacgtt gacagtccgg tgctcgcgga cgtaccccca tggtggacag cgcgactatc
     1981 aatactgagt ggagaacgtg acgatgacac acacgctcga cgatttactg gaagcgttgg
     2041 aagatgtcga tgacgccacc cgcgaggaag cggcaaagac cttggccgaa ctcggcgatc
     2101 cgaagagtct cgatgcgctg gtcagcgcct gtagtgatga ctattggtcg gtgcgtgccc
     2161 atgccggggt gggagcggcc aagatcggtg ggcccaaggc actggaggcc gtgatcgcgt
     2221 tgttcaacga cccgatcatg gaagttcgca accaggctgt cgacgctgtc gtcaagatgg
     2281 gatccacggt tctcgaccgc atgatcgcgg tgatgagaga tgaccgctgg cgagtccggg
     2341 agcatgcggc gaaaacgtgt ggcgagctca aggaccggaa ggccgtcgag gcattgatcg
     2401 tggcctgtcg tgatcgcgac ggggcggtga agagcgctgc ggccgaagcg ctgggcaaga
     2461 tcggcgatcc cgccgctgtg ccggccttga tcaaactttt ccgtgattca tccaagattg
     2521 tgcgcgagac ggccggtacg gccctcgtct acatcggaca ggcgtcggtg gatccgctga
     2581 tcgagacgtt gaaagacaaa gactttgtcg tgcgttgtca tgcggcgcgc gccctcggcg
     2641 ggatgacgac ggactatcaa atcggccgca cgtgggtgcg cgaaccgagg gttgtcgatg
     2701 cgcttattgc catgctcaaa gacccggatc gggctgttcg ggaagatgcg accattgcac
     2761 tgggacagat cggcgacccg cgagcgatcg atgcgctcat cgaagccatg aaagatggcg
     2821 cggtcaagcg gcatgccatc gcttcactgg gcatgatcgg agatgctcg
//
LOCUS       DATDNW010000197         3250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_163815, whole genome shotgun sequence.
ACCESSION   DATDNW010000197 DATDNW010000000
VERSION     DATDNW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163815"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VI359_04920"
     CDS             <1..616
                     /locus_tag="VI359_04920"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973626.1"
                     /translation="SACADLPHLRVLHDPLTPEEHVTLGLTYEIQGHRDLATREYRTA
                     LTQEAGYVPALIGLGNVAFAEGELEESETFYQQALEASPEHPGANNNLAMLYLARGGD
                     LREAERLALRALAHKGPLQPYVLDTLAHVYARQGRYQEAVAALEKAEAVAPSQDKMLR
                     DRLIRLRDQLVGTDAQEDRRTMEQPGVHQSPERWRDRSGEEVAI"
     gene            805..1416
                     /locus_tag="VI359_04925"
     CDS             805..1416
                     /locus_tag="VI359_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973627.1"
                     /translation="MNSKSISLFVITIFMTVGGGEGLVLAQSDTDEVRMQTMQREADD
                     DAVKRGQKAHAELLAKQFQLPASEIEKMRNTGQGWGEVTIRLALADKLVKTDPAKFPT
                     LTAALDRINTLRNEGRGWGNISKELGFKLGPIVSEVRQSLNDLRRDLRAEQVKTGKVN
                     DSGNGADLKREARLEHIERAQRPERPERPQRPERPEKPERVGR"
     gene            1568..2437
                     /locus_tag="VI359_04930"
     CDS             1568..2437
                     /locus_tag="VI359_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY6973628.1"
                     /translation="MLARQSNVHIAIAGIVAFLISPLVSRAVDQPRDQKVQDWQVGFS
                     PTYMSGNYGTSSTTSITYLPLSIRRLFDNGDLTFTVPYLCVTGNGAVTVLSGTPNRVS
                     KSGTATTGQGGGKGKKPGNVEPTSSTECGIGDLIFRGRYYLVDEHGPVPTVAVTGRVK
                     IPTADADRGLGTGRFDEGVGVEMSKKLIGNWMGFVDFGYTFIGDPEGVNLRNQWYYDF
                     GVGYNVTNKLLVSMYYEEYRALIEGLSNPRDLLFALNYTASSSLRFNASILAGLSSGA
                     PDYGLTGGISWRF"
     gene            complement(2459..2911)
                     /locus_tag="VI359_04935"
     CDS             complement(2459..2911)
                     /locus_tag="VI359_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6973629.1"
                     /translation="MLVHDVMSTGVVTAKKTDTVRSVVVKMMNRNCGAIPVVEGDNQL
                     IGMVTLRDVLLPLYPNYGDYIHDNVHSRDFMEMEEGYPEVLGKKVEDIMSRNPLTVSP
                     TTPVLEAASYMGLKNFRRIPVVEKHKLVGMLSIGDINRGLFFERGMQP"
BASE COUNT          729 a          897 c          969 g          655 t
ORIGIN      
        1 gtctgcctgc gctgatctgc cgcatcttcg ggttctccac gatcccctca ccccggagga
       61 gcatgtcaca ctgggattga cctatgaaat ccaaggacac cgagacctag caacccgcga
      121 atatcgcacg gccctgacac aggaggcagg gtacgtccct gccttgatcg ggctgggcaa
      181 tgtcgcattc gccgagggtg agctggagga atcagagacg ttctatcagc aagcgttgga
      241 ggcctctcca gaacacccgg gtgcgaacaa caatttagct atgctctacc tggccagagg
      301 tggggacctg agggaagccg aacggttggc gctccgcgct ctggcgcaca agggtccgct
      361 tcagccatat gtcttggata ccctcgccca tgtctatgca cggcaaggac gataccaaga
      421 ggccgtggca gcgttggaaa aagctgaggc agtggctccg tcgcaagaca agatgcttcg
      481 cgatcgcttg attcgattac gagatcaatt ggtcggtacc gatgctcaag aggatcgaag
      541 aacgatggaa caaccaggag tccatcagag tcccgagcgc tggcgtgata gatctggcga
      601 agaggtggcg atctaatcgg cctgctgttc ggttcgaaac gacgacaggg gtcttctctt
      661 gcgccgcaat ggctaggagg ccggacaatg acagatactc gacaagtgac tcacagtgtc
      721 cctgctttgg caaccgcgag ttgggcgtat cacactcggg caaataaacg catagtgcag
      781 tcagtcaagg aaaggagggg taggatgaac agcaaatcga tcagtctttt cgtgatcacc
      841 atcttcatga ctgtgggcgg cggtgaaggg ctcgtgctgg ctcaatccga taccgatgag
      901 gtccggatgc aaacaatgca acgggaggcc gatgacgatg cggtcaagcg tggacagaag
      961 gcgcacgccg agctgctggc caagcaattc caattaccag ccagtgagat cgagaagatg
     1021 cgaaacacag gccaggggtg gggggaggtc accattcgtc tggctctcgc ggacaaatta
     1081 gtgaagaccg atcctgccaa attcccgacg ctcaccgcag cgttggaccg gatcaacacc
     1141 ttgcggaatg agggaagagg atggggcaac atctccaagg agctgggctt taagctcggg
     1201 ccaatcgtga gcgaggttcg gcagtccctg aacgatttac ggcgcgacct tcgtgcagaa
     1261 caggtgaaga cagggaaggt gaacgacagc ggcaatggag cagatctgaa gcgtgaggcg
     1321 cgcctcgaac atattgagcg ggcccagcga ccggaacgac cggaacggcc gcagcgaccg
     1381 gagcgtccag agaagccaga gcgcgtcggg cgttaggtct cacattgtgc gagtaaacgc
     1441 gggggcagct ccacgggttg gtactgagta acctcgcatc ctaacttgaa gctgtcatca
     1501 gcaccggatc ggcacctgcc ttctggcagg tgccgtcaca cgccaacgac gttcagcagg
     1561 aatagtcatg ctggcacgac aaagcaacgt ccacattgcg atcgctggga tcgtcgcctt
     1621 cctgatcagc cccttggtca gccgcgcggt tgaccagccg agagaccaga aggtgcagga
     1681 ttggcaagtg ggtttctccc ccacttacat gagcggcaac tatggaacca gttcaacaac
     1741 cagcattacc tatctgcctc tgtcgattcg tcgtctcttc gataacgggg atctcacctt
     1801 caccgtgccc tatctctgcg taaccggcaa cggggcggtg actgtgttga gcggtacacc
     1861 gaaccgtgtg agcaaaagtg gaaccgccac cacgggccaa ggtggcggca aaggcaaaaa
     1921 gccgggcaat gttgagccga cttcgtcgac agagtgcggt attggtgatc tcatttttcg
     1981 cggacggtat tacctggtcg atgagcatgg cccggtcccc acggttgcag tgaccgggcg
     2041 tgtgaagatt cccacggcag atgcagatcg tggattgggg acgggaagat tcgacgaagg
     2101 cgtgggggtc gagatgagca agaaactcat cggaaactgg atgggcttcg tcgacttcgg
     2161 atacaccttt atcggagatc cggagggcgt caatctgcga aaccagtggt actatgattt
     2221 cggtgtcggc tacaacgtga cgaacaaact cttggtcagc atgtattacg aggagtatcg
     2281 agcgttgatc gagggcctat ccaacccgcg cgacctcctg ttcgcattga actatacagc
     2341 cagttcctcc ctccgattca acgcgtcgat cttggcggga ctctcaagcg gagcaccgga
     2401 ttatggcctg accggtggga ttagttggcg gttttgacgc tcccaggaaa atcgtgcttc
     2461 agggctgcat gcctcgctca aagaagagcc ctcggttgat atcgccgata ctcagcatgc
     2521 cgaccagctt gtgcttttcc accacgggaa tgcgccggaa gttcttgaga cccatgtacg
     2581 aggccgcttc caagaccggt gtggtgggcg acaccgtgag cggattccgg ctcatgatat
     2641 cctcgacttt cttgcccaac acttcggggt agccctcttc catttccatg aaatcccggc
     2701 tatgcacgtt atcgtgaatg tagtcgccat agttgggata caacggcagc aacacatcgc
     2761 gcaaagtgac catgccgatg agctggttgt ctccttcgac cacgggaatc gcgccacagt
     2821 tccggttcat catcttgacg acaaccgacc ggaccgtatc cgtcttcttc gccgttacca
     2881 ctccggtaga catgacatcg tgaacgagca tggtagcctc cttgcgattc agcactgcat
     2941 ctgctccgtg ccagtcttac tacggtgcgt accgccatag gaggtcacgg gcgatcccgg
     3001 atgaggaaga gtgttctccg tacatcgccc gtggccggcg cttagaacgc caaggaccac
     3061 ctattctatt gtctgtagga ataagacccg cgcgagttga tgggccttgg cggctcgtcg
     3121 gggaacgact ttattcgtcc caggcactcg ggctcttcac gctcgccgca actctctcac
     3181 ctggcgtacg gctcgtatca gcgttcccta cgcgggaaat ggtgttgccc gtggagatct
     3241 ggctcgcgtg
//
LOCUS       DATDNW010000198         3935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_983852, whole genome shotgun sequence.
ACCESSION   DATDNW010000198 DATDNW010000000
VERSION     DATDNW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983852"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VI359_04940"
     CDS             <1..345
                     /locus_tag="VI359_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973630.1"
                     /translation="DEEWLKAEYLVAASRRGARRAMSVANQLRQSKSRVIQCLVKGAD
                     DKVLTDAIAMGEAQRTGAVVVVGAPDTAQDEVLLVDLPSKKVGRPRAKTMKMKDLISL
                     ARRNRRNSKDPS"
     gene            342..1697
                     /gene="dnaB"
                     /locus_tag="VI359_04945"
     CDS             342..1697
                     /gene="dnaB"
                     /locus_tag="VI359_04945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY6973631.1"
                     /translation="MKSMSDVDLSQPKLPPQNLEAEQSVLGAILLENAAMAKAMELLV
                     EDDFYRTSHRKIYRAMLELSDVGEVIDQITLTEQLKAQGALEAVGGSAYVAELVQVVP
                     SSANIRYHCKIVRDKALLRGLIHTSTEVLTRGYEGTTAVDELLDFAERSVFSLAQGKL
                     DRSFSPINHIIKESLDLVDKLHKRNEQVTGVPTGFYDLDDLTAGLQPSDLVVIAGRPS
                     MGKTSLVLGMATHAAIHAGTVVGIFSLEMSKPQIVLRMLSSEAKVDSHGLRTGKLQKE
                     DWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKARRLKAERGLGLLVVDYLQLMQGRS
                     DSESRQQEISDISRSLKALAKELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQ
                     DADVVMFIYREEVYDPNSERKGIADILVSKHRNGPIGKKELFFHDRFAKFESLDNREV
                     V"
     gene            1762..3615
                     /locus_tag="VI359_04950"
     CDS             1762..3615
                     /locus_tag="VI359_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973632.1"
                     /translation="MRVLMSDRIVTPSIVARYTTSGFGISLALILFTVMACAAAPQAP
                     QATVISQPTVKTKPPAHIPDASATYHFMLGYQAESSQDIDRAIQEYLTVLKTDPSAQS
                     VKARLAELYFSLGDFPNAQRYANEAAEGAGQDAQLLTQIAKIFVGVGQGDRAVTLLDR
                     AIEQDPTASEAYFAKGLLLLNLKRLPEAEQAVRAGLARNPESPVGQYHLGRILLEAGK
                     LEEAAASFDRAIAVNQSFEPAYLALASLYEARQDRDKAVGILQKYLRNVNPRNRDIRH
                     HLVRLYVSMKDYKGATKELGELLAEDPTDLDAHLRLALIEGEQKDYAKAINRLTMILK
                     ARPAELKVRDYLGYLYEEAKEPQKALDAYTLNVQLEPSYFEGHLHLGVLFYRLKKFPD
                     SVTHLAEASKLNPKQPESHIVLGLAYLQMEQYEKATQAFEEGIRQNPKSADLYFNLGT
                     AYDKLNRFDEVVRVMEIALQLDPHHADTLNYLGYSYAERGIKIDQALSLTKQAVALKP
                     ENGYYVDSLGWAFFKSGLLTEALTEIKRAVALVGDDPVIFEHLGDIYAKQQRMSEARE
                     AWLHSLELDPSNAKLIERFRDLGMGDPTQEERIQQAKRRVSERIQSQPATQ"
BASE COUNT          984 a          980 c         1067 g          904 t
ORIGIN      
        1 gatgaagaat ggctcaaggc cgagtatttg gtggcggcgt ctcgccgagg ggctaggcgc
       61 gcgatgtcgg tggcaaacca gttgcggcaa tctaaatctc gagtgattca atgtctggtc
      121 aaaggagccg acgacaaagt attgactgat gcgatagcca tgggagaggc ccagcgaacc
      181 ggagcggtgg tcgtcgttgg tgctcccgac actgcacagg atgaggtgct gctcgtggat
      241 ctgccaagca agaaggttgg ccgacctcgc gcgaaaacta tgaagatgaa agatctaatt
      301 tcccttgctc gccgaaatcg tcgaaatagt aaggacccat catgaagtcc atgtcggacg
      361 ttgacctgtc gcaaccgaag ttgccaccgc aaaatctgga agcggagcaa tccgtgcttg
      421 gtgccatcct actggaaaac gcggcgatgg ctaaggccat ggagttgttg gtcgaagacg
      481 atttttatcg gacctcccat cgcaagatct atcgcgcgat gttggaactc tctgatgtcg
      541 gagaagtcat tgatcaaatt acgcttactg aacagctgaa ggcgcaggga gcattggagg
      601 cggtcggtgg gtccgcctac gtagcggaac tggttcaggt cgtacctagc tccgcgaata
      661 tccgatatca ctgcaagatc gtccgcgaca aagcgttgct gcgcggtttg atccacacgt
      721 cgacagaggt tctgacacgt gggtacgaag ggaccacagc agtcgacgag ttgctggatt
      781 ttgccgaacg gtctgttttc agtctggccc aaggtaagtt ggaccgctcg tttagtccta
      841 ttaatcacat catcaaggaa agcctcgatc tcgtcgacaa gctccacaag cgaaacgagc
      901 aagtcacagg cgttcccaca ggtttctacg atctcgacga tttaaccgcg ggactccaac
      961 cgtctgattt ggtggtgatt gcaggccgac cgagcatggg gaaaacaagt ttggttttgg
     1021 gaatggcgac tcatgccgcg attcatgctg gaacagtggt cgggatcttc agcctcgaga
     1081 tgtcgaaacc gcaaatcgta cttcgcatgt taagttcgga ggcaaaggta gactcccatg
     1141 gtttgcgaac ggggaagctt cagaaagaag attggtggcg attggccgag gcagccggtc
     1201 gtttggaaca ggccccgatc tatatcgatg ataccggtgg gatcaccgta caacagatgc
     1261 gaggtaaggc ccgtcggctc aaggccgaac gaggacttgg attgttggtg gtcgattatc
     1321 tccaactcat gcagggaaga agcgattcgg aatcccgtca acaggaaatc tccgatattt
     1381 ctcgttcgtt gaaagccttg gccaaagaac ttaatgtgcc ggtggtggcg ttatcccagt
     1441 tgagtcgtgc agtcgaagct cggaaaccac cgatccctat gctcgctgat cttcgggaaa
     1501 gcggcgccat tgaacaggat gccgacgttg tcatgttcat ataccgagag gaagtatatg
     1561 atccaaactc cgaacggaag ggaattgcgg acattttggt cagcaagcat cggaacggtc
     1621 cgatcggtaa gaaagaactg ttctttcatg atcgttttgc caaatttgaa agtctcgata
     1681 atcgagaagt cgtttgaccc cgcttcactc cccctcgtat aatccgtttg agtcctactc
     1741 gagacgattt ctgagatgat catgcgagtc ttgatgagcg atcggattgt tacaccctcg
     1801 atcgttgcca gatataccac atcgggcttc ggcatttcgc tcgctctcat ccttttcacg
     1861 gtgatggctt gtgcggcagc gcctcaggct ccacaagcga cagtcatttc tcagcccacc
     1921 gtcaaaacga agcctccggc gcacatccct gatgcatcag caacctacca tttcatgctg
     1981 ggttatcagg ctgagtcgtc tcaagatatc gaccgggcga tccaggaata tcttacggtt
     2041 ctcaaaaccg atccctcagc tcaatcagtc aaagccagac tggctgaact ctatttctca
     2101 ctcggcgatt tccccaatgc tcagcgttac gccaacgagg ccgcagaggg agcagggcaa
     2161 gatgcccaat tgttgacaca gatcgccaag atttttgtcg gagtagggca aggagatcgg
     2221 gcggtgactt tattagatcg agcgattgag caggatccga ctgcgagcga ggcgtatttt
     2281 gccaaaggac tgctgttgct gaacctcaag cgtctgccgg aggcagagca agccgtcaga
     2341 gctggccttg ctagaaatcc tgagtctccc gtcggccagt accacttagg acgtatcctt
     2401 cttgaagccg ggaagttgga agaggcggcg gccagttttg atcgggccat tgcggtcaat
     2461 cagtcgttcg aaccagccta cttggccttg gcgtcgctct atgaagcgcg acaggatcga
     2521 gataaggcgg ttggaattct tcagaaatat ctccggaatg tgaatccccg caatcgggac
     2581 attcgtcacc atttggttcg tttatatgtc tcaatgaagg attacaaggg agctacgaag
     2641 gaattgggcg agttgctcgc cgaggaccct actgatctcg acgctcactt gcgtctggcg
     2701 ctcatcgagg gagaacaaaa agactatgcc aaggcaatca atcggttgac gatgatcttg
     2761 aaggcgcgac cggccgaact gaaagtgcga gactatctcg gctatctcta tgaggaagca
     2821 aaagagccgc aaaaagcgct cgacgcatac accttgaatg tacagcttga accgtcatac
     2881 ttcgaagggc accttcatct tggagtgctc ttctatcggc tcaagaagtt tcccgattcg
     2941 gttacacacc ttgccgaggc gtcaaaactg aatcccaaac aacctgagtc acatatcgtc
     3001 cttggattgg cctacctcca gatggagcag tatgaaaaag caacacaagc atttgaagag
     3061 ggcattcgcc agaacccaaa gagcgccgac ctttatttca atcttggcac ggcatatgac
     3121 aagctcaatc gcttcgacga ggttgtgcga gtaatggaaa ttgcgctcca gctggatcca
     3181 catcatgctg acacgcttaa ttaccttgga tatagctatg cggaacgcgg gattaaaatc
     3241 gaccaggctc tgtctctcac gaaacaggcg gtcgccctga agccggagaa tggctattat
     3301 gtggatagcc ttggttgggc cttcttcaaa tcgggattgc taaccgaagc actcaccgaa
     3361 atcaagcgcg ccgttgccct cgttggggac gatccagtaa ttttcgaaca tctaggagat
     3421 atttatgcca agcagcagag aatgtccgaa gcccgcgaag cctggctcca ttcacttgaa
     3481 ttagatccat caaatgcgaa gttgattgag cgattccgcg atctcggtat gggagatcct
     3541 acccaagaag aacgtatcca acaggcaaag cgacgcgtat ccgagcggat acagtctcaa
     3601 ccagcgacac agtagtcacc ttcccccctt ccagccctat tctgactcga ttctgatcgc
     3661 atctttggag tgtctaggcc tcataagcag aggtgtgttc catgtatccg acctgctgat
     3721 cataggacgg aattctgata aaaattggtg aatttcttga tggatcagca gcttaggatt
     3781 gttaaagcct taggcggcag gctgtagcca tctatgcgtg gacaagtttg tgtgcacggt
     3841 caccacaggt gccaattctt ggcagcttcg agcagaaacc ggaagagccg gctcagaaat
     3901 tagatgagaa tcataagggg gagtgtttgc taaca
//
LOCUS       DATDNW010000199         3698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_709779, whole genome shotgun sequence.
ACCESSION   DATDNW010000199 DATDNW010000000
VERSION     DATDNW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709779"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..159
                     /locus_tag="VI359_04955"
     CDS             4..159
                     /locus_tag="VI359_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973633.1"
                     /translation="MTSIHGSHPVAPSGVDVYNPAFDITPAEYITGIITERGVFPPHE
                     LARTFSS"
     gene            257..676
                     /gene="ndk"
                     /locus_tag="VI359_04960"
     CDS             257..676
                     /gene="ndk"
                     /locus_tag="VI359_04960"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY6973634.1"
                     /translation="MSERTLAIIKPDAVKKHVIGDIITRYEKAGLKPVAMKLMHLSKA
                     TAEGFYAVHKARPFFESLCAFMSSGPAVVLVLQGENAIKKNREIMGATDPAKAEKGTI
                     RAAHGANIEYNAVHGSDSPETARFEIGYFFSEMEFVR"
     gene            759..2117
                     /locus_tag="VI359_04965"
     CDS             759..2117
                     /locus_tag="VI359_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973635.1"
                     /translation="MVLSLTGCHHTVHPVAPSIPPVESGVDLRLDTQTRSLEEAYRAY
                     VQGRYPKASILFKRFVETNPQSPRLSEARWWLARSYEAQGNVQAAVAEYRVLAGASVP
                     LDSSAAGYQAHAVRRLDDIMQAGGAATLSEVRPVVLAMTHGDWSRIADLSSWIAKVRQ
                     AGVTTLLIDAGTSMADLDQSRPAGAYFKTSTVPLIDDVLGRVIPRAHAEGVAVFARLD
                     LHQATWMPLNPDWVSAVPGSSRPPQSSDVVDVLHPDYQHAVGRIVDDLCRTGIDGLVL
                     QARMRKGFAEEISPTSWAVFERKFGQSAKGDPTSPLLWRWAGWKTRSYLRFVEQLKEQ
                     VRRERSTRTMVVTVHASAVLDPKASLMDYGEDLLETRLKGFEVLVLPEPEASIGAGSG
                     RTEFMKRLVSTMSGERPLWLGTSLSLSDPEMIPEAISTTLTAMSEQPQTPLVLMNEAI
                     VP"
     gene            2206..2592
                     /gene="gcvH"
                     /locus_tag="VI359_04970"
     CDS             2206..2592
                     /gene="gcvH"
                     /locus_tag="VI359_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY6973636.1"
                     /translation="MIPADLRYHQEHEWVRVSGAQATLGISHFAQDTLGDIVFIDLPK
                     VGASVTAGQQIGEVESTKTTSTIYTPVSGTIAKVNTDLKDHPEVVNSDPYGKGWMAVI
                     ELSKPGEVEKLMTAAQYDTFLKNQKH"
     gene            2660..>3698
                     /locus_tag="VI359_04975"
     CDS             2660..>3698
                     /locus_tag="VI359_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6973637.1"
                     /translation="MTLQKHIAILGAGPGGYVAAIRAAQLGARVTVIENQALGGVCLN
                     WGCIPSKALLSVVELGDKLKKADELGVILPGAARYDLARMVGRKNKVVAGLVKGIATL
                     FKAWNIEQIDGTGSLRDAHIIDVRKKDGTESQITADAIVVATGSSWPNLPQFPIDGRQ
                     ILTSKDLLDLEVIPTRLLIVGAGVEGCEFAALYSGLGTQVTMVELMSRVLPVEDEEIA
                     SMMERELKKRGVTLYTGTTIDQLERSDTTVTVHLKDGTSVTVDKLLVSVGRGFNSRGI
                     GLEQVGVQTGRRGEILVNDRMETNVAGIYAIGDVVGKAMLAHVASAQGKVAVENIMGH
                     PTTVRYDVIPAG"
BASE COUNT          853 a          988 c         1092 g          765 t
ORIGIN      
        1 gaggtgactt ccatccacgg cagccatccg gtcgcaccat ccggtgtcga cgtgtacaat
       61 cccgccttcg atatcacgcc ggcggaatac atcaccggca tcattaccga acggggagta
      121 ttcccaccgc acgaactcgc ccgaacgttt tccagctgac ttgtgtcctt cttgcaaggt
      181 cgtttggcca tccttataat gcagaattat cactgagcca ttgtccgtca ctgaaagggg
      241 aggattactt caagcgatga gtgaacgaac gctcgcaatc atcaagcccg acgcggtaaa
      301 aaaacatgtg attggtgaca ttatcactcg atatgagaag gcaggactca agccggtggc
      361 catgaagttg atgcacctgt cgaaggcaac agcggaggga ttttacgcag tccacaaagc
      421 taggccgttc tttgagagcc tgtgcgcatt catgtcgtcc ggaccggctg ttgtgctcgt
      481 gctccagggc gagaacgcca tcaagaagaa tcgagaaatc atgggggcga cggaccctgc
      541 gaaagccgag aaaggaacca ttcgcgcagc ccatggagcc aacatcgaat ataatgctgt
      601 ccacggatcg gactcgccgg aaacggctcg attcgagatc ggatactttt tttcagaaat
      661 ggagttcgtc agatagcgcc ccatcctggg gggcatgaga ggccttgcca ttcacattca
      721 cacatccgcg aggggcgata ctctggctcg caggagccat ggtgctatcc ctcaccgggt
      781 gtcaccatac tgtccatccc gttgcaccct ctattccgcc ggtcgaatct ggcgttgatc
      841 ttcgactcga tacccagaca agatcactgg aagaagccta tcgcgcctat gttcaaggcc
      901 ggtaccccaa ggcgtcgatc ctgttcaaac ggttcgtaga aaccaatccc caatcaccac
      961 gactcagtga agcccgttgg tggctggccc gatcctacga agcacagggg aacgtccagg
     1021 ccgcagtcgc tgaatatcga gtattggcag gagcctcagt tccattggac agttccgccg
     1081 ccggctacca agcccatgcc gttcgacgac tcgatgacat catgcaagcc ggcggagccg
     1141 ctaccttgtc agaggtgcgt ccggtcgtct tagcgatgac tcatggtgat tggagccgaa
     1201 tcgcagacct ctcatcctgg atcgcgaaag ttcgccaggc gggtgtcacg acgttattga
     1261 ttgatgccgg gacatctatg gctgatctgg atcagtctcg tccagcagga gcctatttca
     1321 agacgtcaac ggtcccgctt atcgatgatg tgctcggtcg tgtgattccg cgcgcccatg
     1381 cagaaggagt cgccgtgttt gcacggcttg atctccatca ggccacctgg atgccactca
     1441 atcccgattg ggtcagtgcg gttccgggtt ccagcaggcc gccgcaatcg agcgatgtgg
     1501 tggacgtcct ccatccggat taccagcatg cggtcgggcg catcgtcgat gatttatgtc
     1561 gaaccgggat cgacgggctt gtattacagg ccagaatgag aaaaggattc gcggaggaaa
     1621 tcagtccaac gtcgtgggcc gtgtttgaaa ggaaatttgg ccaatcggcc aagggagatc
     1681 cgaccagccc gttattatgg aggtgggctg ggtggaaaac gcgcagctac ctccggtttg
     1741 tcgagcaatt aaaagaacag gtacgccgcg agcgctcgac ccgcaccatg gttgtgactg
     1801 tgcatgcaag cgcagtcctc gacccgaaag cgtcgctgat ggactatggg gaagacctgc
     1861 ttgaaaccag actgaaggga ttcgaggttc ttgtgcttcc agagccggag gcatcgatcg
     1921 gagccgggtc cggtcggacg gagttcatga aacgtctggt gtcgacgatg tcgggtgaac
     1981 ggcctctctg gcttggcact tcactctctc tatcggatcc ggaaatgata ccggaggcga
     2041 tcagcacgac gctgaccgcg atgtcagaac aacctcagac cccgcttgtc ctgatgaatg
     2101 aggcgatcgt tccttgacaa aacgggcact ctgccactac gatccgcacg ccgattttgt
     2161 gatgtactag acgctgtaat ttcgccaagc caccgagagg agactatgat tccagcggat
     2221 ctccggtatc atcaagaaca tgaatgggtt cgggtcagcg gcgcacaagc gacgttgggg
     2281 atcagtcatt ttgcgcaaga caccctaggc gatatcgtct ttattgacct cccgaaagtc
     2341 ggggcctcgg tgacagcggg gcagcaaatc ggcgaagtgg aatcgacaaa aaccacctcg
     2401 acgatctata ccccagtcag tggcaccatc gcaaaggtca ataccgattt gaaagaccat
     2461 ccggaagtcg tgaattccga tccctatggg aaaggctgga tggcggtcat tgagctctcg
     2521 aaaccgggtg aagtggagaa gctgatgacg gctgcacagt acgacacctt cctcaagaat
     2581 caaaaacatt gaccgcggta tgatgggttc gttggcatcg tgccctcgct gaaccagtcg
     2641 gcaatggtcc gatgagatca tgaccctcca aaagcacatc gccatcctcg gtgccggccc
     2701 gggcgggtac gtcgcggcga ttcgagcggc tcaattaggg gcgcgcgtga cggtcatcga
     2761 gaatcaggcg cttggcggcg tttgtctgaa ctggggctgt atccccagca aagcgctgct
     2821 gtccgtggtc gaactgggcg acaaactgaa aaaggcggat gagctgggag tcatcctgcc
     2881 tggggcggct cggtacgatc tggctcggat ggtcgggcgc aaaaataaag tggtggcagg
     2941 gttggtcaaa gggattgcga cgctcttcaa agcttggaac atcgaacaga tagacggaac
     3001 cggatcgctc cgtgatgcac acatcattga cgtccggaag aaggatggga ctgaaagcca
     3061 aattacagcc gacgccatcg tggtcgcgac cggttcgtcc tggcccaacc tgccgcagtt
     3121 tccgatcgat gggcgacaga tcttgacgag taaggacctg ttggatcttg aagtcatccc
     3181 cactcggctc ttgattgtcg gtgccggtgt cgaaggctgt gagtttgccg ctctctatag
     3241 tgggctaggc actcaagtca cgatggtgga actcatgtcg cgtgtgttgc cggttgagga
     3301 tgaggaaatt gcgtcgatga tggagcggga actcaagaag cggggagtca cgttgtacac
     3361 cgggacgaca attgaccagc tggaacgatc cgatactacc gtgacggtgc atctcaaaga
     3421 tggtacatcg gtgacagtcg acaaacttct cgtctccgtg ggccgagggt ttaacagccg
     3481 cggcatcgga ctagagcaag tcggtgtcca gaccggtcgg agaggggaaa ttctcgtcaa
     3541 tgaccggatg gaaaccaatg tggcgggaat ctacgcgatc ggcgatgtgg tgggaaaggc
     3601 catgcttgca cacgtggcct cggcgcaagg gaaagtcgcc gttgagaata tcatgggaca
     3661 tccgaccacg gtccgatatg acgtaatccc ggccggaa
//
LOCUS       DATDNW010000200         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_94046,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000200 DATDNW010000000
VERSION     DATDNW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94046"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..622)
                     /locus_tag="VI359_04980"
     CDS             complement(29..622)
                     /locus_tag="VI359_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973638.1"
                     /translation="MHQITVSVVAAFVVIGLSESAGRAIDVQPTSDQIQAALGRGKEA
                     AERRSPPDSFYVRFGATDELHPNGFLITKTGSLSIMATHMALRGLQPSEADVAQVLEG
                     KTMLISTVIFGNVPNFAVDSYMVLDQGGKTIKPVTVRFDGQANRSAAWPENPRFKAKV
                     VASFNYADFDPLAKTNITVFPANGGETTFTLNFSEIH"
     gene            784..1497
                     /locus_tag="VI359_04985"
     CDS             784..1497
                     /locus_tag="VI359_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY6973639.1"
                     /translation="MSSPEQTPAPKRQYVNFVFYKVDPAWRRLPEDERTKGKQEFLRA
                     VEDYSGKVLVIAYSAVGIRGDCDIMLWRISYELELFQEMSTKILASGLGKYLTTPYSY
                     LSMTKRSIYVDHHTHENQESKRLTVVPGKAKYIFVYPFLKTREWFLLTKAARQGMMDE
                     HIEVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDT
                     PIFTCIRRSLKEALDTLGG"
     gene            1558..2262
                     /locus_tag="VI359_04990"
     CDS             1558..2262
                     /locus_tag="VI359_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973640.1"
                     /translation="MTMTAPLPPRTSIDSRAALFSALLPGLGQFTQKRYGLGLTCLIS
                     TLALAAASGGLGHISGRAAEIFFFMLLVLPWWAIQSYDAYLGPSDQQWGLLRTWRAVW
                     QRGHDIRFLGALFLLSAVNDSIIILSNPEYLLPFFCTKPAGVVGVATKAVSPVLHTAV
                     GLGFLRLRRWSLFLYLLYAAYGMANAVVNLTCFGPGRVRNTLLITLAGFTIYIVWRRR
                     CFDEITDLERLTRFDR"
     gene            2272..2535
                     /locus_tag="VI359_04995"
     CDS             2272..2535
                     /locus_tag="VI359_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973641.1"
                     /translation="MLPVETDGQPVTKARNSMRDNNTLYAIRYPDGSVSLYIDEEYAM
                     ERGVNPATLTRVEIPRELFSTGTIQDIREYVATYLEAQQTGTA"
     gene            2557..3735
                     /locus_tag="VI359_05000"
     CDS             2557..3735
                     /locus_tag="VI359_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973642.1"
                     /translation="MASPLTVATIESAIAAMPVQGRIMLRLILLQYLDVTQEEIEYMA
                     ADRPDPRCVTGTKPTHNMITKEAIKAVRDKRDEYRRSVRLLRERTWLQCECLKQLASL
                     HDAMANRAGQLLLSRFGQDRDALDALRAQARTALPKPAIRALDRRWDADEITAEEYQR
                     QRLAIELQTHLRLAERYRKRLDLAERERQTASNNSLQDHEIGHIWGIPAGSLAARKVK
                     YLAQYLQMLQTLIQREAPAKDTVTPPLDLWKETFSVLAQLPVERSLSTYDGLERTESA
                     LLDKLASMVWGSLTEEVESKFWLSLVHGGTSNALLSEWGRSLFGLQRLAAILAEVDTS
                     SASLEEALLTRIAPRTKTEPGALLEEKPAEPVLGEMGEHVLRSMLGESHPDLHGSRRS
                     "
     gene            3878..4273
                     /locus_tag="VI359_05005"
     CDS             3878..4273
                     /locus_tag="VI359_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY6973643.1"
                     /translation="MHPWRISFLTLPTLLVIMSAIGVSASLLEIAELLAHPEQYDRQE
                     IVVTGQVSNLQLATNRQGQPAYGFLLKDQAGTLKVISLGQAEVREGEQVIVEGVFSRL
                     RQAGRTIIYNEIKATSIKPINRLNPDLVG"
     gene            complement(4301..>4539)
                     /locus_tag="VI359_05010"
     CDS             complement(4301..>4539)
                     /locus_tag="VI359_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEY6973644.1"
                     /translation="ELIMRIKETVGKAAERAIPPDAVVRAVVHALTASRPRTRYLVGT
                     DAKIRAWMVKWLPDRLNDRLLTWILHLPRDGGRS"
BASE COUNT          977 a         1341 c         1215 g         1006 t
ORIGIN      
        1 gggcatggat ggcagagcgt cgtggaaatt agtgaatctc tgaaaagttc agtgtaaagg
       61 ttgtttcacc tccgttggcc gggaacactg tgatgttcgt cttagccaac ggatcgaagt
      121 cggcatagtt gaacgaggcc accaccttgg ccttgaaccg aggattctcc ggccaagcgg
      181 cactgcgatt cgcctgcccg tcgaatcgca cggtcacagg cttgatcgtc ttcccaccct
      241 gatcaagcac catataactg tccaccgcga agttcggcac gttgccgaag atcaccgtgc
      301 tgatcaacat ggtcttacct tccagtacct gcgccacatc ggcttcactg ggctgcaatc
      361 cccgtaaagc catgtgcgtc gccatgatcg acagacttcc cgttttcgta atcagaaacc
      421 cgttcggatg gagctcgtcc gtggcaccga accgcacgta gaacgagtcc ggcgggcttc
      481 gcctttcggc cgcttccttt ccccgaccga gtgcagcctg aatctggtct gaagtgggtt
      541 ggacgtcgat cgcccggccc gccgactcag acaggccgat gacaacgaat gcggcaacga
      601 cgctgaccgt gatttggtgc atctgttggg cctatatctg ttgatgccag taccagatcg
      661 attttgctct gtcaacgcag gccgtttcag tctctcgccc gttgacaaat cgaaagatga
      721 agtgataaac acacaaggct atacttaccg tgccttttca agaagttaca cggaggagtt
      781 ccgatgtcga gtcctgagca gaccccagcc cccaaacgcc aatacgtcaa tttcgtcttc
      841 tataaagtcg atccggcctg gcgccgcctt cctgaggacg agcgcaccaa aggcaagcaa
      901 gagttcctgc gcgcggtcga agattacagc ggcaaagtgc tcgtgattgc atactcggca
      961 gtcggaatcc gtggcgactg cgatatcatg ctctggcgca tcagctacga actggagctc
     1021 ttccaggaaa tgagcaccaa gatcctggcg tcgggactcg gcaagtatct caccacgccc
     1081 tattcgtacc tttcgatgac gaaacggtcc atctacgtcg atcatcacac ccatgagaac
     1141 caagagagca agcggctgac ggtggtgccc ggcaaggcca aatatatttt cgtctatccc
     1201 ttcttgaaaa cgcgtgagtg gttcctgctc acgaaggcgg cgaggcaagg catgatggac
     1261 gagcacatcg aagtcggcca tcgcttcccc tcggtcaagc tgaatacgac ctactcgttc
     1321 ggtttggatg atcaagaatg ggtggtcgcc ttcgagagcg acaagccgga agactttctc
     1381 gatctcgtca tggccttgcg cgagacggaa ggttctcgct atacacttcg cgatactccg
     1441 atcttcacct gtatccgccg gagcctcaag gaagcgctcg acacgctggg cggataacgg
     1501 aattcgcgtg atgacttctg aacggccttc gattttacca tcgaaggccg tttctttttg
     1561 accatgacgg cgccgcttcc cccccgcacg tccattgatt ctcgggccgc actcttctcc
     1621 gcactcctcc ctggtttggg acagttcaca cagaagcgat acgggttggg tctgacttgc
     1681 ttgattagta cactcgcctt ggcagctgca agcggcggtc ttggccacat ttccggccgc
     1741 gccgccgaaa ttttcttctt catgctgctg gtgctcccgt ggtgggcaat ccaaagttat
     1801 gatgcatatc tcggcccgag cgaccagcaa tggggtctgc tccgcacctg gcgtgcagtg
     1861 tggcagcggg gacacgatat ccgtttcttg ggtgccctct ttctcctgag tgccgtgaat
     1921 gactcgatta tcattctgag caaccctgaa tacctgttgc cgttcttttg cacgaaaccg
     1981 gctggtgtcg tgggggttgc gaccaaagcc gtttctcctg tgttacatac cgcggtgggc
     2041 cttgggttcc tgcgccttcg ccggtggtca ctctttctct atctgctcta tgccgcttat
     2101 gggatggcta atgcggtcgt gaacctcacc tgctttggcc cgggccgagt tcgcaacact
     2161 ctgctgatca cgctcgccgg atttacgatc tatatcgtgt ggcggcggcg gtgttttgac
     2221 gaaattaccg atctggagcg gcttacacgc tttgacaggt gaacaagccc tatgttacct
     2281 gtagagacag acgggcagcc cgtcacgaag gcgcgtaatt caatgcgtga taacaatacg
     2341 ttatacgcca tccgctatcc cgacggatcg gtgagcctct atatcgacga ggaatatgcg
     2401 atggaacgag gtgtgaatcc cgcaaccctg acccgcgtag aaattccgcg agaattgttc
     2461 agtaccggaa ccattcagga catccgcgaa tacgtcgcga catacctaga ggcccaacag
     2521 accggcactg cgtaattgag aaactggaac ctcttcatgg cctctccgct caccgttgcc
     2581 acaatcgaat cggccatcgc cgcgatgccc gtccagggtc gcatcatgct gcgactcatt
     2641 ctgctgcaat atctcgacgt cacccaggag gaaatcgagt atatggcggc agatcgtccg
     2701 gatcctcgtt gtgtgaccgg gacaaaacca acgcacaaca tgatcaccaa ggaagccatc
     2761 aaggcggtgc gcgacaaacg cgatgagtac cggagatccg tccgtctcct gcgcgaacgt
     2821 acatggttgc agtgcgaatg cctcaagcaa ctggcatccc tccacgacgc catggccaat
     2881 cgtgccggcc aactgctgct ctcgcgcttt ggccaggacc gagatgcgct cgacgcactc
     2941 agagctcaag cgcgcacggc tcttcccaaa ccagccatcc gtgcgctgga ccggcgatgg
     3001 gatgccgacg agatcaccgc cgaggagtat cagcggcaac gattggcgat cgaactgcag
     3061 actcacctgc ggcttgccga aaggtatcgc aaacgtttgg atcttgccga gcgcgagcgc
     3121 caaaccgcaa gcaataattc gctacaggat catgagatcg gccatatttg ggggataccg
     3181 gcaggtagtt tggccgcgcg caaggtcaag tatctggcgc aatacctcca gatgctgcag
     3241 accttgattc agcgggaagc gcccgcgaag gacacggtga ctcctccgct cgatctttgg
     3301 aaagaaacct tttcggtcct cgctcagttg ccggtcgaac gatcgctttc tacgtacgac
     3361 gggctggaac gtaccgagtc ggctctcctc gacaagctgg catcgatggt atggggaagc
     3421 ttgactgagg aggtcgagtc aaaattctgg ctctcattgg tgcatggcgg tacttcgaac
     3481 gcgctcctct ccgaatgggg gcgctccctg ttcggattgc agcggttggc tgccatcctg
     3541 gccgaggtgg atacttcttc cgcttccctc gaagaagcgc tccttacccg catcgcgccg
     3601 agaacgaaaa ccgaacctgg tgccctctta gaagaaaaac cggccgaacc ggttctggga
     3661 gaaatgggag agcacgtcct caggagcatg ctcggcgaga gccacccgga cctccacggc
     3721 agccgccgat catgatcgca tatacctgac ttcgaggttc acaacctcac tccccccagc
     3781 gtgactcctc gatgaatctt tggttctgct cgagcgcccg ggattttccc caaatgttcc
     3841 cttgaatttc gacaaaacga cgatataata cggccgtatg catccttggc gtatatcctt
     3901 tcttactctt cccactctac tcgtcatcat gtcggcgatc ggtgtatcag cgagtctgct
     3961 ggagatcgcc gaattgcttg cgcatcccga gcaatacgat cgacaggaaa tcgtcgtgac
     4021 tggccaggtc tccaatcttc agctggcgac gaaccgccaa ggccagccgg catatggatt
     4081 tctgctcaag gatcaggcgg gaacgctgaa agtgatcagc ctcggtcagg ctgaggtacg
     4141 cgaaggtgaa caagtgatag tcgaaggtgt gtttagtcgt ctccgtcagg ctgggcgaac
     4201 cattatctac aacgaaatca aggccacgtc cattaagccg atcaaccgcc tcaatcctga
     4261 tctcgttggt tagttcagtt cttcaaccag ttcaattccg tcacgacctg cccccatcac
     4321 gaggcaagtg taaaatccac gtcagcagcc gatcgttcag gcgatccggc aaccacttca
     4381 ccatccaggc ccgaattttc gcatcggttc ccacgagata acgtgtgcgc gggcgtgatg
     4441 ccgtcaacgc atggacgaca gctcgcacga cggcatcagg tggaatcgcc cgttctgcag
     4501 ccttcccgac cgtttctttg atccgcatga tcagctctg
//
LOCUS       DATDNW010000201         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_164480, whole genome shotgun sequence.
ACCESSION   DATDNW010000201 DATDNW010000000
VERSION     DATDNW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164480"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VI359_05015"
     CDS             complement(<1..424)
                     /locus_tag="VI359_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973645.1"
                     /translation="MDVYSPDLIELVDCLANRRLLPGYLEIFKASTSALTFIRQHLSG
                     IALSYHGEGLWVTQPESRDHPTFDDAAAEAAAHLNVLQSPWLNHECATKQMAGYSFGT
                     YLPPLFTPVSAEVVAQNIIVVQAALDAQCCRPDGSTPLF"
     gene            679..1437
                     /locus_tag="VI359_05020"
     CDS             679..1437
                     /locus_tag="VI359_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6973646.1"
                     /translation="MSTADKYPPRTTNPTPTVVAHMMTPGVVQIPGDVSVSEAALMLE
                     REQTPCLLVKDSDTLFGLMTPTDIVKKVVAQGLEPHDIEARTIMTQPVQFIEYDRALE
                     EATALMTSTGASHLIVTKDNQPVGVLSARDLMLTPPPRRTRIPAMLSLSENGSVVGTA
                     PHSATIIQLGHLGAQLESGTPLDAGTNILLVFSLPGQHTPLTIQGTVLASKKDRVGNA
                     SNIVDIQFTNVATTDQTRIKAWALQTLPHPQDRS"
     gene            1531..>2067
                     /locus_tag="VI359_05025"
     CDS             1531..>2067
                     /locus_tag="VI359_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D ubiquinol oxidase subunit II"
                     /protein_id="HEY6973647.1"
                     /translation="MKSTPARPRTTLSKEETLDQVRLLLDRPDDDLPAALLKELLGGV
                     LRLHEAHLDVLDLKILNRAMKELRHAFRVFHNYRGRRKVSIFGSARTMSSDPNYQLAF
                     LFAERIVEAGFMVITGGADGIMRAAQEGAGRENSFGVNIMLPFEQGPNATIADDPKLV
                     TFKYFFTRKLMFQKEANAI"
BASE COUNT          485 a          603 c          559 g          420 t
ORIGIN      
        1 gaaacaatgg ggtcgacccg tcaggtctac aacattgggc atccagcgca gcctgcacca
       61 cgataatgtt ttgagccacg acttcggcgc tcactggcgt gaacagtgga ggcaagtagg
      121 tgccgaacga atagcctgcc atctgcttcg tcgcgcattc atggttcagc caggggctct
      181 ggagcacatt gagatgtgcc gcggcctcgg ccgcggcatc atcaaacgtg ggatgatcac
      241 ggctttctgg ctgagtcacc cacagtcctt ctccatgata ggagagggcg atgcccgata
      301 ggtgctgcct gatgaacgtc agcgcgctcg tcgaggcctt aaaaatctcg agatagcccg
      361 gcaagagcct tcgatttgcg aggcagtcca cgagttcgat caggtcggga gaatagacgt
      421 ccaccgagag tccaaggccc tgagggaaaa tcgctcggag ccgatccgca aaagctcggt
      481 ttgcatttcc gaagacagtc aagaatccac ccttgtgctg tgccaaaatt tggcctagtc
      541 tagtcgccaa agccgttgtt tgacaaggga agaccttgat cctgaccaag agttccggtc
      601 tgctatactt ggcttacgcc gacgggggcg aaaccctcac gaaccgggga aaaccgcgac
      661 aggagcggtt tcgaacccat gtcaacagct gataagtatc cccctcggac cacgaatcca
      721 acaccgacgg tggttgcaca tatgatgaca ccaggggtcg tacagattcc gggcgacgtc
      781 tccgtaagtg aggcggcgct catgctcgaa cgcgaacaga cgccctgtct tctggtgaaa
      841 gatagcgaca cactgtttgg actcatgact ccaaccgaca tcgttaagaa agtggtcgca
      901 caggggctgg agccccatga tattgaggct cgtacgatta tgacgcaacc ggtccagttc
      961 atcgaatacg accgtgcgtt ggaagaggcc accgcactca tgacgtcgac gggagcctct
     1021 cacttgatcg tcacaaaaga caaccagccg gtcggcgttc ttagcgcacg cgatctcatg
     1081 ttgactccgc caccgcgtcg tactcggatt cccgcgatgc tcagcttgtc agagaacggg
     1141 agcgtggtcg ggacggctcc gcacagtgcc accattatcc aactcggcca tcttggggcg
     1201 cagcttgagt caggcactcc gttggatgca ggaacgaaca tcctcctcgt attttcgttg
     1261 cccggtcagc acacaccctt gacgattcag gggacggtgc ttgccagcaa gaaagaccgt
     1321 gtgggtaacg cttcgaacat cgtggatatc caattcacaa atgtggcgac gacagaccag
     1381 acacgtatca aggcgtgggc attgcaaaca cttccgcatc ctcaagatcg ctcctgacgg
     1441 gtctcctcca ttgctttgac caatcatgaa tcagctgata gaattttgtc tctaccacga
     1501 accgcgcatt ctgctgagat gaatgatccg atgaaatcca caccagctcg tcctcgaacc
     1561 acgttgtcga aagaggaaac gctcgaccaa gtccgtctcc tcctcgatcg ccccgacgac
     1621 gatcttcccg cggcgctact gaaagaactt ctcggagggg tccttcgcct gcacgaggcc
     1681 catctcgacg tcctggacct gaaaatcctg aatcgggcga tgaaggaact ccgccacgca
     1741 tttcgcgtat tccacaacta tcggggccgc cgaaaggtga gcatcttcgg ctcggcacgc
     1801 acgatgtcga gcgatcccaa ctatcaactg gcgttcctct tcgccgaacg tatcgtggaa
     1861 gccggtttca tggttatcac aggcggtgcg gacggaatca tgcgagctgc gcaggaagga
     1921 gccggccgcg agaacagctt cggcgtcaac attatgctgc ccttcgaaca ggggcctaat
     1981 gcgacgatcg ccgacgatcc caagctggtc acgtttaaat acttctttac gcgcaaactc
     2041 atgtttcaaa aggaagccaa tgccatc
//
LOCUS       DATDNW010000202         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_785542, whole genome shotgun sequence.
ACCESSION   DATDNW010000202 DATDNW010000000
VERSION     DATDNW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785542"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VI359_05030"
     CDS             complement(<1..563)
                     /locus_tag="VI359_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY6973648.1"
                     /translation="MLVFLIHIRDPQFYALPAKTRAKNGNVRVMGFPPIGVMSLSSVL
                     KQAGHDCVMFDQANPDTPNDVIIEMIKRQEPALVGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLDLLAHLNDPEGVASLTWTR
                     DGRVVNNPGRPMERHLDQWPFPDRESL"
     gene            complement(1185..1841)
                     /locus_tag="VI359_05035"
     CDS             complement(1185..1841)
                     /locus_tag="VI359_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6973649.1"
                     /translation="MGRKRATPFDPKIFLTKVDGGKQILKCRKKQALFSQGEAADSVF
                     YILGGKVKLTVLSSKGKEAVIAILGQGEFFGESCLAGQLVRTAAATALEESAIMRIDK
                     AAMIRVLREEPTFAELFMSHLLSRNIRIEEDLVDQLFNSSEKRLARILLLLAHFGKES
                     KVEELIVPKISQETLADMVGTTRSRVSFFLNKFRKLGFIDYNGGLRIRSSLLNIVLVD
                     "
     gene            complement(1870..2742)
                     /gene="sucD"
                     /locus_tag="VI359_05040"
     CDS             complement(1870..2742)
                     /gene="sucD"
                     /locus_tag="VI359_05040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY6973650.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKVVAGVTPGKA
                     GQEVEGIPVFNTVRDAVRKTQCDTSLIFVPPPFCADAILEAADAGIKLVICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKKGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAIVMIGEIGGDAEEKA
                     AEFIKKNVKKPVVSFIAGITAPPGRRMGHAGAIISGGKGTAAEKMKTLEAAGVKVVKN
                     PAEIGNAVKAALGR"
BASE COUNT          609 a          830 c          773 g          632 t
ORIGIN      
        1 ttaaggctct cacgatcggg aaacggccat tgatccagat gccgctccat cggacgaccc
       61 gggttgttca ccacacgacc gtccctcgtc catgtcaggc tggccactcc ctctggatcg
      121 ttcagatgcg ccagaagatc gagcagcagt tgttctccat ccccacggca gacaaaatcg
      181 acttctggac actgtagctt caccagtccg gcattcagac tggcaaacac gccgccaaag
      241 gctaatttca cggttgaatc ggctgcgcga atttgacggg cgaggatctt cgcgtaggga
      301 tagcttgtgg tgctgagaaa gctcagaccg accaacgccg gctcctgccg cttaatcatt
      361 tcgatgatca cgtcgttcgg cgtgtcgggg ttcgcttggt cgaacatgac gcagtcgtgt
      421 cccgcctgtt tcagcacgga cgacagcgac atgacgccga ttggcgggaa ccccatcacc
      481 cgaacattgc cgttcttggc acgggtttta gccggaaggg cgtagaattg aggatcgcgg
      541 atgtggatca ggaagacgag catacctacc tccaagcgcg gaatgattca cacgccgtct
      601 taccgcgagc cggccttact gcttccggct gagacaatgc cggaccgcaa ctttcgcgtc
      661 ctggttcggg gttctttcag gggaaccaaa acgacggggt aactgtcaat tccgaggaga
      721 gagtgcttcc tgctttctaa tctgccgaga ggcttcggcg cagatcccgt agaaggtgtg
      781 atcctaactg gtgagaggaa gttagcacga ttaagcacgc aaggaaagga aataccatcg
      841 ctggggccca tcaccggctc tcgggtgctg aggcagcatg atcgtagctc gttcaggcgg
      901 tccgcagccc ggtcatcatt cactgcgagc aatcggtatc tcatcatcac cgctgtcatt
      961 tgaattttca tcctctcaac agaacgaccg attctccaac gctcggtgaa cctcaccgta
     1021 agaaggtgcc ggagaatctc tatggacatc gtgtgaattt gagtgaaatt atgccagaga
     1081 cacagaatgg cagactggtc aaaattgctc acttttgagg aatggatgcg gccggcaggc
     1141 gaggctgcgc tttgagccta agaccctacc cgaaacatgg gaagttagtc gaccaggacg
     1201 atgttgagaa gggaactgcg aatgcgcaat ccgccgttgt aatcgatgaa gcccagcttc
     1261 ctgaacttgt tcagaaagaa actgactcgt gatcgcgtgg tgccgaccat gtcggccaga
     1321 gtttcctgac tgattttcgg aacgatgagt tcttccacct tgctttcttt cccaaaatgg
     1381 gcaagcagca gcaaaatccg agcgagtcgc ttttcgctgg aattaaagag ttgatccacc
     1441 aggtcctctt caatccttat gttacgagac aggagatggg acatgaacag ctccgcgaac
     1501 gtgggctctt cgcgaaggac gcggatcatg gcggctttgt cgatgcgcat gatcgcggat
     1561 tcttccaggg cagtcgcggc ggcggtacga accaattgcc cggcgagaca ggattcgccg
     1621 aaaaattctc cttgcccgag aatcgcgatg acggcttctt tgcctttgga ggagaggacg
     1681 gtcagcttga ctttgccgcc caggatatag aatacggaat ccgctgcttc cccctgcgaa
     1741 aacagcgcct gcttctttcg gcacttcaga atctgtttcc cgccgtcgac tttcgtgagg
     1801 aagatttttg gatcgaacgg agtggcgcgt ttgcgtccca tacatgctac ccgcgagttt
     1861 gattaagtgt cagcggccaa gcgccgcttt gaccgcattc ccgatctcgg caggattctt
     1921 cacgaccttc acgccggccg cttcgagagt tttcatcttc tcagccgcag tcccttttcc
     1981 gccggaaatg atcgccccgg cgtggcccat gcggcggccg gggggagcgg tgatcccggc
     2041 gatgaagctg acgaccggct tcttcacgtt cttcttgatg aactcagccg ccttttcttc
     2101 cgcatcgccg ccgatctcgc cgatcatgac gatcgcctga gtttccggat ctttctcaaa
     2161 gagcggcaac acatcgacaa acccggtccc attcaccgga tcgccgccga tgccgacaca
     2221 agtcgtttcg ccaagtccta gcgtcgacag ctggtgaacc gcctcatacg tgagagtccc
     2281 actgcgggat accacaccca ccacaccttt tttatggatg aagccaggca tgataccgat
     2341 cttcgcctca tcgacggtga tgacaccggg acagttggga ccgatcaagc gaacatcgcg
     2401 gccatggagc gctcgtttca ccttgaccat atcgttgacc ggaatccctt cggtgatgca
     2461 gatgacgagc ttgatccctg catcggccgc ttccaggatg gcatccgcgc aaaacggcgg
     2521 cggcacaaaa atcagggagg tgtcgcactg agttttcctg accgcgtcac ggactgtgtt
     2581 gaagacgggg atgccctcga cttcttgacc ggctttcccc ggcgtgacac cggctacgac
     2641 cttggtcccg taagccttgc actgggtcgc gtggaacgaa ccttccttgc ccgtaattcc
     2701 ctgcaccacc acccgcgtat tcttattgac gagaatgctc acaatcccct cttctttcct
     2761 tcgtccccca ccaagacggg ggaggctgag tctctaaatc ctcttgagcg aggggcacag
     2821 ggttgagccc ccactgcgcg catc
//
LOCUS       DATDNW010000203         5474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_845296, whole genome shotgun sequence.
ACCESSION   DATDNW010000203 DATDNW010000000
VERSION     DATDNW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845296"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="cax"
                     /locus_tag="VI359_05045"
     CDS             complement(<1..873)
                     /gene="cax"
                     /locus_tag="VI359_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY6973651.1"
                     /translation="MRHVFASWLDLLLVFVPVTVGLELLHIDPLVVFLSAGLAIIPLA
                     GLLGRATEHLTSHVGAGVGGLLNASLGNAAELIIALAALRSGLHDVVKASLTGSILGN
                     ILLVLGASMVAGGLKYERQKFNQTAAGMGASLLLLAAVGLIIPALFHFTAADRGVVRE
                     QELSLAIAVVLFAIYVLSLMFSLKTHKHVFRGDPHAADDLAETRWTRNTSIVVLTGAT
                     LLVAVMSEILVGALEPAAHRLGMTQLFVGLILVALVGNAAEHSTAVMVALKNKMDLAF
                     GIAVGSSLQIALLIA"
     gene            910..1590
                     /locus_tag="VI359_05050"
     CDS             910..1590
                     /locus_tag="VI359_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973652.1"
                     /translation="MKPPLGPHALYYPFHLCHPETVHRLLARFATIHFRDFMALQLTP
                     MAGMTAFHDRMGADFPDLVANGRLVQGYDVSGPLSSDFLVAIDRDLADTSWRRLFHQA
                     LCQDRRFQRGLFDLTHSMKLGKTTVPGAAALLNLMEAAFQQHRFQVEDIKALSSAPLT
                     VEQGYRYEYGLALIKTSASLIYTDHLARRHHLQTVTDSPAHFALFEHSARRDGRIWAN
                     HLLVRQGY"
     gene            1625..1921
                     /locus_tag="VI359_05055"
     CDS             1625..1921
                     /locus_tag="VI359_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEY6973653.1"
                     /translation="MATTPPPLAIPHTAETPTTGTGNGLEARVIVYNCDCHTYQQVIA
                     LFCQCIPGMSSSKAFELAWKIDHDGEAVVFAGTLKESEDIAAKLGAGGLKVSVQ"
     gene            complement(1928..2254)
                     /locus_tag="VI359_05060"
     CDS             complement(1928..2254)
                     /locus_tag="VI359_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973654.1"
                     /translation="MNQRRRLLNAAKKGDQKAVNRLFELYQVRIFSGEMVNKLNRAAG
                     LHKLRGKGAQQKLPKGKALSKQSTPSHKAASKVKTRSTHGKVHPKTVKPKKLKKAHKP
                     KKSKKR"
     gene            2477..3151
                     /locus_tag="VI359_05065"
     CDS             2477..3151
                     /locus_tag="VI359_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HEY6973655.1"
                     /translation="MEHHDKPETTVSDDPHARELLRRAFENTARWQPDFKGFTADLTV
                     NINGKETSGPVIVKGPREVSVQLSDGDVQKWAQEQLGMIAVHRGPRSFEESDGKYTLT
                     AEEDGHPLGTKLNIHGSNSFYRLKNNRITQINRTMAHHGMAPFAFTINVEESAITQDQ
                     KNLTTKYSVYYYSPADGKLNNVESFTDTHVRVGASDLPATRRIISFENGQVTVKSLTF
                     TNHRLL"
     gene            3155..3595
                     /locus_tag="VI359_05070"
     CDS             3155..3595
                     /locus_tag="VI359_05070"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEY6973656.1"
                     /translation="MDLRKSFPRSMRFKLEGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     LDFRLMEFAGITDQQFTATVKANPTDEDVAQWFRGVAKPHAGSELEEWNRMMLYRGPS
                     TPEKQAYFNKLRDAVDPSRTDLTAWSDLQDLEEGRVVPKRQRVA"
     gene            3766..5181
                     /locus_tag="VI359_05075"
     CDS             3766..5181
                     /locus_tag="VI359_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973657.1"
                     /translation="MVKSRIWVLGALVLGVVATVMAESGAPTFLSNLFPFPNSTGILK
                     TFSATGKLDLTGPFFQSLGTNGRSCATCHQSSDAWSVSATHVSERFEETKGLDPIFRT
                     NDGSNCDHDIDTSSEKGRRQAYSLLISRGLIRIALPVPDGAEFEVVSISNPYGCNEST
                     TLSMYRRPLPATNLRFLSTVMWDGRESSAQMGTKPITFATNPNDLLFDLAHQSVDATL
                     GHAQATTPPTPHQQQQIVAFEMGLSTAQAIDFGAGSLDSTTAKGGPVALVTQPFFVGI
                     NDPLGQNPFKTPFIPVIFTLFTPSWAHAHSHDDQATHRARIVNGQALFNSRTINITGV
                     GGLNSPSLPLIKGTCGTCHDAPNVGNHSVSAPLNIGVADITNSLGVSYLPVITLRLKA
                     DPSQVVSTTDPGRALVTGQWADIGKFKGPILRGLAARAPYFHNGSAASLKEVIEFYDT
                     RFEMGLTDKEKADLVAFLSVL"
BASE COUNT         1255 a         1603 c         1414 g         1202 t
ORIGIN      
        1 agcaatcagc aaggcgattt ggagactcga gcccaccgca atcccgaacg ccaaatccat
       61 cttgtttttt agcgcgacca tgacggcggt cgaatgttct gctgcattcc ccaccaatgc
      121 aaccagaatc aacccgacaa acagctgcgt cattcccaaa cgatgggcag cgggttccag
      181 cgcaccaacc aggatttcac tcatgaccgc caccaacaga gtcgcgccgg tcaggacgac
      241 gatcgaggtg ttgcgggtcc aacgcgtttc tgccaaatcg tcagcggcat ggggatctcc
      301 tctaaacaca tgcttgtggg tcttcaatga aaacatgagg ctcaggacat aaattgcgaa
      361 taacacaaca gcaatggcca gactgagctc ctgctcgcga accacgcctc gatctgccgc
      421 cgtaaaatga aacagcgcgg gaatgattag tcccactgcc gccagcaaca agagactggc
      481 tcccattcca gccgccgttt ggttaaactt ctgtcgttca tacttcaatc cgccggcaac
      541 catggatgcc cctaagacga ggagaatgtt cccgagaatg gacccagtga gcgaagcttt
      601 caccacatcg tgcaatccgc tgcgtaacgc cgccaaagcg atgatcaatt ccgcagcatt
      661 gccgagtgac gcgttcagca atccaccaac cccagcaccg acgtgggacg tgagatgctc
      721 tgtcgcccgt ccgagcaagc cggctaaggg gataattgcg agccctgctg aaagaaacac
      781 gacgagtgga tcaatgtgaa gcagttcgag tcccaccgtg acaggaacga agacgagcag
      841 gagatcaagc cacgaggcaa agacgtgtcg cacgagcaac ccctataaca tgacgccatg
      901 cgtttgtcga tgaaaccgcc tcttggaccc cacgcactgt actacccgtt tcacctttgt
      961 catccggaga cggtccatcg gctgctggca cgatttgcca caatccattt tcgcgatttc
     1021 atggcgctcc agctgacacc aatggcgggg atgaccgcct ttcacgatcg gatgggcgca
     1081 gactttcccg acctcgtggc caatggccgc ctcgtgcagg gatacgacgt gagcggccct
     1141 ctcagtagcg acttcctcgt tgcgatcgat cgtgatcttg ccgatacaag ctggcgccga
     1201 ctcttccatc aggccctgtg ccaggatcgc cggtttcagc gaggactctt tgatctcacc
     1261 catagcatga agctagggaa aacaacggtg cccggagcag cggctctcct caacctgatg
     1321 gaagctgcat ttcagcaaca tcgctttcag gtggaagaca tcaaagcact cagctccgct
     1381 cctctcacag tcgagcaagg gtatcgttat gagtacggcc tcgcgcttat caagacctcg
     1441 gcctctctga tctataccga ccacctcgcc cgcaggcacc atctccagac agtcacggat
     1501 tcccccgccc attttgcctt gtttgagcat tctgcgagac gcgatggccg tatatgggct
     1561 aatcatctgc tggtgaggca gggatattaa tcctttccca gaagtctgct agaattctac
     1621 ccctatggct acgacgcccc cccctcttgc catcccacat acggcggaga caccgacaac
     1681 cggaaccggt aatgggctgg aagcgcgtgt cattgtctac aattgcgact gtcacaccta
     1741 tcagcaagtg atcgccctat tttgccaatg catcccgggc atgagttcgt ccaaggcatt
     1801 cgagttagcg tggaagattg atcacgacgg cgaggcggtg gtgttcgccg gaacgttgaa
     1861 agagtcggag gatatcgccg ccaaactcgg cgcgggagga ttgaaggtgt cggtccagta
     1921 acgcagacta gcgcttcttt gacttcttgg gtttgtgagc tttcttcagt ttctttggtt
     1981 ttacagtttt ggggtgaacc ttcccgtgcg ttgaccgtgt tttcaccttc gaggcagcct
     2041 tatgagaagg agtcgactgc ttcgagagag ccttcccctt gggcagcttc tgctgagctc
     2101 ccttgccacg cagcttgtgc aacccggcag cccgattcag cttattgacc atctcgccgc
     2161 tgaagattcg tacctgatag agctcaaata gtctattgac ggccttttgg tcgccctttt
     2221 tcgccgcatt caggagccgg cggcgttgat tcatttcctc ttcttctgta tgcgaggcag
     2281 ctcgtctctc catgcccatc ctttccgtcg aatttcggcg caacgcggcg cgcagtataa
     2341 cggaaacccc gcaattgttc tagagcgatt cattctcctc gtcgcctctc tcctctgctt
     2401 gtgatcccat ttctggcatg ggtataatcc atcaacgttt gccctaatga gatgtgttca
     2461 gcaaggaggc caagccatgg aacatcacga caaaccagag acgactgtta gcgacgatcc
     2521 tcacgcccgc gagttattgc gccgggcctt tgagaatacg gcgcgttggc agccagactt
     2581 taaggggttt acggcggatt tgaccgtgaa catcaacggg aaagagacca gcgggccggt
     2641 gatcgtgaag ggcccccgcg aggtgtcggt gcaactctcc gacggcgacg tgcagaagtg
     2701 ggcgcaggaa caactcggca tgatcgccgt tcaccgaggg ccacgcagct ttgaagaatc
     2761 ggatgggaaa tataccttga ccgccgaaga ggacggccat ccgctcggca cgaaactcaa
     2821 catccacgga tcgaattcat tttatcgcct taagaacaac cggatcaccc agatcaatcg
     2881 cacgatggct catcacggca tggccccctt tgcctttacg atcaacgtgg aggaaagcgc
     2941 gatcacccaa gatcagaaaa atctgacgac gaagtacagc gtgtattact attcgccggc
     3001 agatggaaaa ctgaacaatg tcgaaagctt caccgacacg catgtgcgtg taggagcgtc
     3061 cgacctaccg gccactcgcc gaattatttc cttcgagaac ggccaagtaa ccgtcaagag
     3121 cttaaccttt acaaaccacc gtctgctgta gcgtatggac ctcagaaaga gtttccccag
     3181 aagtatgcgg ttcaagctgg aaggctacgt ccatcttgcg cgcatgatcg acaaatgccg
     3241 ggctgtgctg gccggcaccg agggggagta tatctatccc tgcccgctgg acttcagact
     3301 catggaattc gccggcatca ctgatcagca attcaccgcg acagtcaaag cgaatccaac
     3361 cgatgaggat gtcgctcaat ggtttcgcgg tgtggcgaaa ccacacgcgg gatcagaact
     3421 cgaagagtgg aatcggatga tgttgtatcg ggggccgagc acccccgaga aacaggcata
     3481 tttcaacaag ctccgtgacg cagtcgatcc ttcacgcacc gacctcaccg cctggtccga
     3541 cttgcaggac ctcgaagaag gccgggtcgt gccaaagcga caacgagtcg cctagacaca
     3601 aaactcgtat ccttctctcg cctcaagcga tcatcctaca cgcagacgcg aggtaagccg
     3661 tagatatttt attttcgaat atacggtgga gatctacgta tttagcgtaa tttacatcct
     3721 ggacgacatc ctatagcatc gcgccgctca caaaggaggc cgtccatggt gaaatctcgc
     3781 atctgggtcc tgggcgcgct cgtgttggga gtcgtggcga cggtaatggc cgagagcggt
     3841 gcccctactt ttctctcaaa tctctttcca tttcccaact ccactggaat tctgaaaacc
     3901 ttcagcgcga caggcaagct ggacctgacc ggtccatttt ttcagagtct cggtaccaat
     3961 gggcgtagtt gcgcaacctg ccaccaatct agtgacgctt ggtccgtctc ggccacacac
     4021 gtctccgaac ggtttgaaga aacgaagggt ctggatccga tttttcgcac caatgacggc
     4081 tcgaattgcg atcacgatat cgacacgtct tcggagaaag gccggcggca ggcttacagc
     4141 ttgctgatca gtcgcggcct gatccgtatc gcgctcccgg tgccggacgg ggctgaattt
     4201 gaagtagtca gtattagcaa cccctacgga tgcaatgaat ccacgactct ttccatgtat
     4261 cgacgcccgc ttccggcgac caatctccgg tttctcagta cagtgatgtg ggacgggcgt
     4321 gaatcgtccg cccaaatggg gacaaaaccc atcacatttg caacgaatcc caacgaccta
     4381 ctcttcgacc tagcacatca gtctgtcgac gccaccctcg ggcacgccca agccaccaca
     4441 ccacccacac cgcaccagca gcagcaaatt gtggcgtttg aaatggggct gtcgaccgcg
     4501 caggcgattg actttggtgc tgggtcgctc gattcaacca cagcaaaggg gggacctgtg
     4561 gccctcgtca cgcagccatt tttcgtcgga atcaatgatc cgctgggcca gaacccattc
     4621 aaaacgccgt ttatcccggt gatcttcaca ttattcacgc cctcttgggc ccacgcacat
     4681 tctcatgatg accaggccac tcatcgtgcc agaattgtga acggccaagc actgtttaac
     4741 tcgcgcacta tcaatatcac cggagtcggc ggactcaatt ctcctagctt gccactcatt
     4801 aagggtacat gcggtacctg tcatgacgcg cccaatgttg gcaatcactc agtctcggcc
     4861 ccgctgaata tcggcgtcgc cgacatcaca aattctcttg gtgtgagcta cttgcctgtc
     4921 atcacgctgc gcctcaaggc cgacccatct caggtggtct ccacgactga cccgggccgg
     4981 gccttggtga ccggtcaatg ggccgatatc ggaaaattta aagggccgat tcttcgagga
     5041 ctcgccgccc gggcacccta ctttcacaac ggctcggcgg caagtctaaa ggaagtcatt
     5101 gagttctacg acacccgatt cgagatgggg ctcacggaca aggaaaaggc tgatctggtg
     5161 gccttcttga gtgtcttgta agaacagacg actcgaccgc ctcgtttgtt ttcatgacaa
     5221 tcgatggata agccattggg tttgatgcca aggagcactg tacttcttct cataagacaa
     5281 agcgctgatt ctctctcagg attctgatat tcagatttat cgcggtatga gcggaggggc
     5341 cgtaggggga aagggatacg tctctttcaa cgccaaggcg acggcatcgg ccactcgaat
     5401 gtcgctccgg ctgtgctcgg taatgctgtc gtatcgagct tgcgtcgttt cgaccggcat
     5461 ctgcttgttg tact
//
LOCUS       DATDNW010000204         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_786579, whole genome shotgun sequence.
ACCESSION   DATDNW010000204 DATDNW010000000
VERSION     DATDNW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786579"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VI359_05080"
     CDS             complement(<1..477)
                     /locus_tag="VI359_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEY6973658.1"
                     /translation="MKPVAMIALTVVLTSMPISRALAANSDNGPGCGLGKLAWSDFKR
                     QKDIAPQVLMATTNGTFGSTTFGISSGTSGCTNDGKVWAEERTNVFVAATFDGLTAEM
                     AQGEGEHLTSLATMMGVPVEQHAQFFALAQERYQELVRAGDTSPAAMIKALSDAMVS"
     gene            complement(545..1120)
                     /locus_tag="VI359_05085"
     CDS             complement(545..1120)
                     /locus_tag="VI359_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY6973659.1"
                     /translation="MKQRGAVRIGVIADTHGLYDPAVEEHFAGVTAIVHAGDIGDRAV
                     IESLQGIAPVVAISGNVDDYERSGYPRQIVIRQQGKIVAVRHVLYENGKLMEEAITWL
                     DSLQPDVCIFGHSHRPTIARYGRTLLFNPGSAGPRRFTLPRAIGILTITRNRVVPTHV
                     RLNDKPTIGHRGKSKRRTTVAKSFREQRSSV"
     gene            complement(1117..>2036)
                     /locus_tag="VI359_05090"
     CDS             complement(1117..>2036)
                     /locus_tag="VI359_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY6973660.1"
                     /translation="TLYVKDPVLIRAFREAAPPELTHHFEAAAAKVRQARTHRRRMWG
                     IIVGSLGTLALFLWLGSDWLVDLAVSRIPVEWEQKLGESAYRDFLAKQDVLKDGPAVA
                     AVIEMTQRLTSQVTKSPYTFEITVVKSDVVNAFALPGGYIVVFTGLMKKAESGEEVAG
                     VLSHEVNHVLQRHGLDRIVKTLGLMAVVTILVGDPQGLAGLMKQVGLELVTLKFGRAQ
                     ETEADLTGLQLLQRAKIDPAGMIRFFERLSEKDQGRMEWLSTHPMSTARAERLKAELA
                     SLPKTIPEPFTFDWKKIQESLGVQPVAMP"
BASE COUNT          424 a          619 c          522 g          471 t
ORIGIN      
        1 gctgaccatg gcatcgctga gcgctttgat catcgcggcc ggcgaggtgt ctcctgcgcg
       61 aacgagttcc tgatatcgtt cttgagcaag agcaaaaaac tgcgcgtgct gctctaccgg
      121 cacacccatc atcgtcgcca atgatgtcaa atgctctcct tctccctgag ccatttcggc
      181 agtcagaccg tcgaaggttg cagcaacaaa aacattggtc cgctcttctg cccaaacttt
      241 tccgtcattc gtgcagccgg acgtgccgct gctaatcccg aatgtcgtgc tgccgaaggt
      301 gccattggtg gtcgccatga gtacttgtgg ggcaatatct ttttgtcgct taaaatccga
      361 ccatgccagc ttgccaagac cgcatcctgg tccgttatca gaattcgcgg cgagagcgcg
      421 tgagataggc atgctcgtta gtacgaccgt cagagcaatc atcgcaacag gcttcatatt
      481 gtcctcctct tctccatgaa attgtccgtg gtactaaacg atttggaccg tcattttgga
      541 ccgatcatac actgcttctt tgctcccgaa aagattttgc tacggttgtg cgtctcttcg
      601 attttcctct atgtccaatt gttggtttat cattcaatcg cacatgcgtc gggacgacac
      661 ggttacgggt aatcgtcaga atcccgatcg ctcgaggaag ggtaaaccgt cgaggaccag
      721 ccgatcctgg attgaagagg agcgtgcgtc cataccgagc gatcgtcgga cgatggctat
      781 gaccgaagat gcacacatcg ggctgtagcg aatccagcca tgttatcgcc tcctccatca
      841 gctttccatt ctcatacagc acgtggcgca cggcaacgat cttgccctgc tgacggataa
      901 cgatctgtcg aggatatccg ctccgctcgt agtcgtcgac gttgccagat atggccacca
      961 caggggcaat accttgtaac gactcgatca cggctcgatc gccgatgtct cccgcatgca
     1021 caatcgcggt cacgcccgca aaatgttctt caacagcggg atcgtagagc ccgtgtgtat
     1081 cggcaatcac tccgatgcga acggctccgc gttgcttcat ggcatggcca ccggctgaac
     1141 accgagcgac tcttgaatct ttttccaatc gaatgtaaac ggctccggga tcgtctttgg
     1201 cagagatgcc agttcggcct tgagccgttc agcacgggcc gtgctcatcg gatgtgtcga
     1261 gagccattcc atccgcccct gatctttttc tgacagtcgc tcaaagaatc ggatcatgcc
     1321 ggctggatcg atcttcgccc gctgcagcag ttgcaaccct gtcagatcgg cttcggtttc
     1381 ctgtgcccgg ccgaacttga gcgtcaccag ttcgaggccg acctgtttca tcagaccggc
     1441 caagccctgc ggatcaccga caaggatcgt cacgaccgcc atgaggccaa gcgttttcac
     1501 gatgcgatca agcccatgcc gttgcaagac atgattcact tcgtgactga gcaccccggc
     1561 aacttcttca ccgctctcgg ccttcttcat gagaccggta aaaaccacga tgtagccgcc
     1621 cggcaacgcg aaggcattga ccacgtcgct tttcactacc gtgatctcga acgtatacgg
     1681 gctcttggtc acctggctcg tcagccgttg ggtcatttcg atcaccgccg caacggccgg
     1741 cccgtctttc agcacgtcct gttttgcgag aaaatcgcga taggccgatt ctccgagttt
     1801 ctgttcccac tccacgggaa tgcgactcac ggcgaggtcg acgagccaat cggaacccag
     1861 ccagagaaac agtgcgagag tccccagcga gccgacaata atcccccaca tccgccgccg
     1921 atgagtgcga gcttgtcgaa ccttggcggc cgccgcttca aagtgatggg tcagctcggg
     1981 tggcgccgct tctcgaaagg ctctaatcag gaccggatcc ttcacgtaca gcgtcc
//
LOCUS       DATDNW010000205         4654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1249849, whole genome shotgun sequence.
ACCESSION   DATDNW010000205 DATDNW010000000
VERSION     DATDNW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249849"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VI359_05095"
     CDS             complement(<1..930)
                     /locus_tag="VI359_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY6973661.1"
                     /translation="MQQSSSTSTQPTALEKVIAFVDERVGLKTLQAKMLNEPVPGGSR
                     WAYIFGSILLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHDVDYGWFLLSYHFWG
                     STAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGLVMGFGFTGYLLPWDQRAYWA
                     TTVGVEIMDKTPVVGDFMARFLKGGPTPGQMTLSRFFVIHVMILPAALMGLAGLHIFL
                     FRKAGPAGPFRGSVEELKVKSDYFFPRQVWKDIVGMVTVFLSICALAFWEPVVLLEEA
                     TPDPGDYHPEPEWYFLFLFQMLRLKIFAGEFGQF"
     gene            complement(937..1869)
                     /locus_tag="VI359_05100"
     CDS             complement(937..1869)
                     /locus_tag="VI359_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY6973662.1"
                     /translation="MQPKLKSKVRAADREIGEVTRVIMDPLSREVSHIVVSMNGSGER
                     QIAMAHVQSVSDHLVELRSAASEILALPAFKREDFVTTHEVEIAHLEENIHVTPGEVL
                     VPFPELEKSVKRRTFFMNFTHAIGFLIGLPIAYPVLRYLMKPMYQPFDNSWLKIGNVG
                     KIKQEDIGVQFKYRKKVKEAYMPEAEIEKNVWILKATPAVLEKVYQGKDMEFRDATGT
                     SVWVNKKEVPYIAFSGKCPHLGCGYKWRQHKILGQVFLCPCHLSIYDASGKVLDGPAP
                     RPLDALPIKISAGGDVQIVDMEFKAGTKAQIRIV"
     gene            complement(1871..2677)
                     /locus_tag="VI359_05105"
     CDS             complement(1871..2677)
                     /locus_tag="VI359_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6973663.1"
                     /translation="MGNVIKKLVIGLVVGGTLFGVTKALEFPTVFQMMFFAYAILGAV
                     VFILLDAPSLKPLGGVKALGALLVFYIVLCVAYIAGASLWPQYDPEDEKGKIDKILAP
                     KRKATEQGKAEELIARAKALDEQAKALAERIKALGGDQASAAQGAGGTGAPPATAGAA
                     GGDILKLGEDQWQLQECYNCHKLRGEGGKKRGPELDNIGSFMTVEDIKQKILDPRSWM
                     ADGFEKEYEKKKMPDKYKDLMEEKDVEALASWLVTFKNTSVNTPKPIKKK"
     gene            complement(2716..4419)
                     /locus_tag="VI359_05110"
     CDS             complement(2716..4419)
                     /locus_tag="VI359_05110"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY6973664.1"
                     /translation="MKTWQRCLFALCALVALFGGAAYAQAPGAPPVEFPYTGNRTAVW
                     IVAQLHILFAAFILGAPIFVVISEWLGYRKQDPRYDRLAKEVMKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLVFAVIYPLLFIGETILLYLYFYSWDAWKGEKKARH
                     IALGVLLNLIGTITLFVIDGPTSFMNTPVKAEGVSPAEFLATATLWDKIYNYSWMPMN
                     LHRLVGNVTFGGFITGLVAAYMYMAAKKEEERSYYDWMGFVGNLIGVGALLFLPFMGY
                     LLAYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSMKRIEGVERVR
                     MTIFAPIVMVALPFVMTYVWTIFPVPDPLSLAILIPLALLPVVLGRFIPLTVSSRTVI
                     KVGFLMVVVGDAIWLTPHGFVPTGAKLVADLELPSDWNFLALMPAKNSAAFTLVFVTV
                     VNYIIYNRAISQGTIVWGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHTYNL
                     LPDFTAESFTPTLSYSAWWITAITVAFYVVVSFAIFVTLRPSEAKGHAPEGSPAPAAA
                     K"
     gene            complement(4458..>4654)
                     /locus_tag="VI359_05115"
     CDS             complement(4458..>4654)
                     /locus_tag="VI359_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973665.1"
                     /translation="KAWKSKLSEDDRWKVMAFERTFGLKGKAWDATKKDWVSLDGAAA
                     PAVAPATAPAPAGAPASAGK"
BASE COUNT         1088 a         1388 c         1231 g          947 t
ORIGIN      
        1 gaactgcccg aactcgccgg cgaaaatctt gagccgaagc atctggaaca ggaacaggaa
       61 gtaccattcg ggctccggat ggtaatcacc cgggtccggc gtggcttctt ccagcaacac
      121 caccggctcc caaaaggcca gagcgcagat cgacaagaac acggtcacca tgccgacgat
      181 gtccttccag acctgccggg gaaagaaata atcgctcttg accttgagct cttcgaccga
      241 gccacggaaa ggcccagccg gccccgcctt gcggaacagg aaaatgtgca agccggccag
      301 ccccatcagg gcggccggaa gaatcatcac gtggatgaca aagaaccggc tgagagtcat
      361 ttgacccggc gtcggtcccc ctttgagaaa ccgtgccatg aaatcgccga cgactggcgt
      421 cttatccatg atctcgacgc cgacagtggt cgcccaatag gcccgctgat cccaggggag
      481 caggtagccg gtaaatccga atcccatcac aaggccgaag agggccaagc cgaccagcca
      541 gatcaactcg cgcggctttt tatacgcgcc ccacaagaat acttgcgaca tgtgcgcgaa
      601 gacacacacg accatggcgg tggagcccca gaaatgatag ctcagcagaa accagccata
      661 gtcgacgtca tggatgatgt attgggtgct cgcataggca tggtcggcgg tcggcacata
      721 gtaaaacatc agcaaaatgc cggtgacggc ctgcatgatg aagatgaaca gtaagatgga
      781 gccgaagata taggcccatc gcgagccgcc ggggaccggc tcgttcagca tcttggcttg
      841 aagtgtcttt aggccgacgc gctcgtcaac gaaggcgatg actttttcga gagcggttgg
      901 ctgcgtgctg gtcgaagaag actgttgcat ggacgtctag acgatccgaa tttgcgcttt
      961 cgtcccggcc ttgaattcca tgtcgacgat ctggacgtcg cccccggccg agatcttgat
     1021 cggcaaggcg tccagaggac gcggggccgg tccatcaaga actttccctg aggcatcata
     1081 aatgctgaga tgacacggac agaggaacac ctgtcccaga atcttgtgtt gccgccactt
     1141 atagccgcag ccgagatgcg ggcacttgcc cgagaatgcg atgtagggca cctctttttt
     1201 gttgacccac acgctcgtgc cagttgcatc gcggaattcc atatccttgc cctgatagac
     1261 cttctccagc accgccggcg tcgccttcaa gatccagacg ttcttttcga tttcggcctc
     1321 gggcatgtag gcttctttga cctttttcct gtacttaaat tggactccga tgtcttcctg
     1381 cttgatcttg ccgacgttgc cgatcttcag ccaggaattg tcaaacggct gatacatcgg
     1441 cttcatcaga taccggagga cgggataggc gatcggcagt ccgatcaaga acccgatcgc
     1501 gtgagtgaag ttcatgaaga aggttcgccg cttgacgctc ttctcaagtt cagggaaagg
     1561 caccaaaact tcgcccggcg taacatgaat attctcttca agatgcgcga tttccacttc
     1621 atgtgtcgtg acaaagtcct cgcgcttgaa agcagggagg gccagaattt ccgacgccgc
     1681 agaccgaagc tcaaccagat gatccgagac ggactgcaca tgtgccatcg cgatttgccg
     1741 ttcgccgctt ccgttcatgg acacgacgat gtggctcact tcgcgggaca acgggtccat
     1801 gatgacccgc gtgacttctc cgatctcgcg atccgccgca cgaactttgg acttgagctt
     1861 gggttgcata ctactttttc ttgatcggct tcggcgtgtt caccgaggta ttcttgaacg
     1921 tgaccagcca agaggcgagc gcctcgacgt ccttttcctc catgagatcc ttatatttgt
     1981 ccggcatctt cttcttctcg tattcctttt cgaacccatc tgccatccag ctgcgagggt
     2041 caaggatctt ctgctttata tcctcgaccg tcatgaagct gccgatgtta tcgagctcag
     2101 gaccgcgctt ctttccgccc tcccctctca gcttgtggca gttgtagcac tcttgtagtt
     2161 gccactgatc ttcgcctagc ttgagaatgt cgccaccggc cgcgcccgca gttgccggcg
     2221 gggctccggt ccccccggct ccctgagcag ccgatgcctg atctcctccc aacgccttaa
     2281 tccgctcggc cagcgctttg gcctgctcgt cgagtgcttt ggctctggcg atcaattctt
     2341 cagccttgcc ctgctccgtc gcttttcgtt tcggagccag aatcttgtcg atcttccctt
     2401 tttcatcctc cggatcgtac tgcggccata gcgacgctcc cgcaatatac gcaacacaga
     2461 ggacgatata gaagactagg agggcaccca acgccttcac accgccaagg ggtttgagcg
     2521 agggcgcatc cagcaggatg aagaccacgg caccgaggat cgcataggca aagaacatca
     2581 tttggaagac cgttgggaac tcgagcgcct tggtgactcc aaacagcgtt cctccgacga
     2641 ctagcccgat taccaacttc ttaatcacgt tacccataga gatccctttt ccttagtggg
     2701 cgttccgaag cggagtcact tggccgcggc aggagctgga ctcccctccg gcgcatgtcc
     2761 tttcgcttca gaaggcctga gcgtgacgaa gatggcgaaa ctaacgacca catagaaggc
     2821 aacggtgatc gcggtaatcc accaggccga ataggacaga gtcggcgtga aggattccgc
     2881 cgtgaaatcc ggaagtaaat tgtaggtgtg gaaatacttc cgcagcagcg atcgcaccgc
     2941 ccccatcaac cccattgtcc aaattgcgct gaaggccagg aagaccagga cgaactgcga
     3001 cgcaaaatcg atcttgcccc agacaatcgt gccctggctg atggccctgt tataaatgat
     3061 gtaattcacg acggtcacga acaccaaggt aaatgccgcc gaattcttcg ccggcatcag
     3121 cgccaaaaaa ttccaatccg acgggagctc cagatcggcc accaatttgg cgcccgtcgg
     3181 gacaaagccg tgtggggtca accagatcgc gtccccaacc acgaccatca ggaaaccgac
     3241 tttgatgacc gtgcgggagg acaccgtcag cgggatgaat cggccgagca ccaccggtaa
     3301 taacgcgaga ggaataagga tcgccagcga gagcggatca ggcaccggga aaatggtcca
     3361 gacatacgtc atcacaaagg gcaacgccac catcacgatc ggagcgaaga tcgtcattct
     3421 cactcgctcg accccctcga tgcgtttcat gctgagccag atgtaataat tgctggccag
     3481 gaagatgagt cccaccatgg ccccttgcat ctcgaagaac atggagagct ggtccgccat
     3541 catgtaggga cagatcgagg catcgtagtc acacagttca taagccagca gatagcccat
     3601 gaacgggagg aacaacaacg caccaacacc gatcagattt ccgacaaagc ccatccaatc
     3661 gtaataggac cgttcttcct ctttcttcgc tgccatgtac atgtaggccg caaccaaccc
     3721 cgtgatgaat ccaccgaacg tcacattgcc gacgagccgg tgtagattca tgggcatcca
     3781 actgtagtta tagatcttgt cccacagcgt ggctgtcgca agaaactcgg caggggacac
     3841 gccttccgct ttcaccggtg tgttcatgaa cgacgtcgga ccgtcaatca caaacaaggt
     3901 aatggtcccg atcaggttga gcaggacacc caacgcgata tgtcgggcct tcttctcccc
     3961 tttccaggca tcccaggagt agaagtacag atacagcaga atcgtttccc cgatgaacaa
     4021 gagcggatag atcaccgcga agaccaaaaa gaaatgattg atcaaccagg tggtgaactg
     4081 cgggtaggtc gccaacaaca cgaagatgaa caggccaccg gtgagggcag tcatgctgta
     4141 gagaatgacc gtgaccttca tgacctcctt cgccagacgg tcgtatcggg gatcctgttt
     4201 ccgatacccc aaccactccg aaatgacgac gaagatgggc gcgccgagga tgaaggcggc
     4261 aaacaggatg tggagttggg cgacgatcca gactgcagtg cgattacccg tgtagggaaa
     4321 ctccactgga ggcgcgcctg gagcctgggc atatgccgct cctccgaaca gcgccacaag
     4381 cgcacagaga gcgaacagac aacgctgcca tgttttcatg atgtttcgcc gcccccccta
     4441 cctagagtaa agtcagccta cttgccagct gacgccgggg cccccgcagg agctggagcc
     4501 gtcgctggtg cgacggctgg cgcagcagca ccatctagcg acacccagtc cttcttcgta
     4561 gcatcccacg ctttcccctt cagtccgaag gttcgctcga aggccatcac cttccaacga
     4621 tcgtcctctg atagcttgct cttccacgcc ttca
//
LOCUS       DATDNW010000206        10374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_909841, whole genome shotgun sequence.
ACCESSION   DATDNW010000206 DATDNW010000000
VERSION     DATDNW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909841"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VI359_05120"
     CDS             complement(<1..213)
                     /locus_tag="VI359_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY6973666.1"
                     /translation="MAETLRINKFFTEHGICSRREADRLIEGGRVTINGRPAKLGDQI
                     AVGDVIARDGQVIPWGNKAIYIKYHKP"
     gene            complement(231..1163)
                     /gene="speB"
                     /locus_tag="VI359_05125"
     CDS             complement(231..1163)
                     /gene="speB"
                     /locus_tag="VI359_05125"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY6973667.1"
                     /translation="MTLPAGWLGAGHNFLGLEEPWCHPERAGVYVLPAPYEHTSSYIL
                     GSDQGPSAIIEASHQVEFYDEQLQCEPYREWGGIATAAALELDGKIDGPAVDAIEAFV
                     RPHVNTGRFLVTLTGEHTGALGAIRAHARRYPNLCVVQIDAHGDLRKAYQGNPYSHAS
                     VMARVVDDGLPLVQVGIRSICPEEIERIKSTDCISTFFAATLLDPSGPYEGRATKWIP
                     DVVKACRGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLNFVTALANGPGILGMDV
                     SEIAPIEGFVAPQFSIARLIYRILGRIRAGRRVH"
     gene            complement(1245..1817)
                     /locus_tag="VI359_05130"
     CDS             complement(1245..1817)
                     /locus_tag="VI359_05130"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEY6973668.1"
                     /translation="MVPTHMFLSRGVGVHREKLASFEQALRSAGVAYCNLVSVSSILP
                     PNCKIIPRKRGETLLNPGEITHCVMARSETNERNRLISASIGLAIPTDRHTYGYLSEH
                     HAYGETDEESGEYTEDLAAQMLATTLGIEFDSNIAWKEREQVFKMGGKIVRTLNITQS
                     AIGKPNRWTTVVALAVFIPLENLPKKVRRR"
     gene            complement(1943..3382)
                     /locus_tag="VI359_05135"
     CDS             complement(1943..3382)
                     /locus_tag="VI359_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6973669.1"
                     /translation="MQGPRPFLIGGQWRYGDSVVRVNDPFTGQVIAEVSQASQADAEA
                     AIQSTVDAASAMASLPSHARYHLLQKIAGALYDRREEFARLMTAEAGKPITDARREVN
                     RAIQTFTVAAEEAKRIPGEVIPLDWTPGTDGHLGILRRFPIGPVLGITPFNFPLNLVA
                     HKVAPALAAGNPILIKPAPQTPLTALLLGEVAMEAGVPPGGLNVVPCDNTIAEHLVID
                     PRFKLLSFTGSAAVGWMLKAKCGKKKVVLELGGNAGVIVEADADLDAAAQRCATGGFG
                     YAGQTCISVQRIFVHHSVADLFTTKLLLQVARLKAGDPNDEATVVGPLINPSAAARVE
                     GWIGEAVSEGARVLLGGKRMGSVLEATVLSNVTPTMNVSCQEVFGPVVTLTSYRQFDE
                     AIAALNHSDYGLQAGVFTQDINKVFHAFRHLEVGAVLANEIPTFRADHMPYGGVKDSG
                     SGREGVKAAIEEMTEPRLLVLNLKSPGVA"
     gene            complement(3773..4423)
                     /locus_tag="VI359_05140"
     CDS             complement(3773..4423)
                     /locus_tag="VI359_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY6973670.1"
                     /translation="MMVVGLTRIIGLVLFLISLTLDPLAVAGIGEVTDDGRIRGRVDA
                     PVTLIEYSDFTCGYCLKFFRDTWPKLQAKYVETGKLRFVYRDYPRADRGVGVEAAVAA
                     RCAGAQGRYWAMHDRLFGEGRRLDSEAFRSYARTIGLDQVVFAQCFEERRYLESIFQD
                     RQEANRWGFHGTPGFILMHTVGGATEKEPAVAIPGAFPFDDFAEEIERLLRSAPRS"
     gene            complement(4420..5526)
                     /locus_tag="VI359_05145"
     CDS             complement(4420..5526)
                     /locus_tag="VI359_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY6973671.1"
                     /translation="MAAREFHDGAKDGLEPLEPLDPEKIRSFEDLLTAMRKTAFGGRR
                     LGEAFEVLWSMIEDPDCSVILTLSGAMTIAKMGKIVSTMIDHGMVQGIVSTGALIAHG
                     LSESVGKTHYRHRPSMSDEELFRKGYNRVYDTLEMEANLNYVEHVVAQTMKRINHDQP
                     LSSEILTRELGKTLAEEFEGDGILKSAYVKKVPVYIPAFTDSEMGLDVGTWAMGREID
                     RARSQAKGDNDLEVLRFINRSCPSFNPYLDLNSYTNVVTSAKRLGIFTIGGGVPRNWA
                     QQVGPYIEIANMRLGLNVKPPRFQYGVRICPEPDYWGGLSGCTYQEGISWGKFVPPEE
                     GGRFAEVLSDATVVWPILMVGLLERKKAKQSKRT"
     gene            complement(5832..5909)
                     /locus_tag="VI359_05150"
     tRNA            complement(5832..5909)
                     /locus_tag="VI359_05150"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5871..5873),aa:Glu,seq:ctc)
     gene            6100..6636
                     /locus_tag="VI359_05155"
     CDS             6100..6636
                     /locus_tag="VI359_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973672.1"
                     /translation="MNEQLQTNKIENGDSAELGEEAKPETGLELHMGSNVFRNINGVV
                     KLQGKEQMFWEVVPDLETLLLTMDLYDDKGVRVGHIRRNVIKSGEIGRFTVTANLHAN
                     ARPDSAPSVTVSDRSNGQTVVEAFLVQGRKIRIAAGSFYTHKGELVQISPHYCRIGTG
                     RTLFGDVVESRGGNALID"
     gene            complement(6661..7011)
                     /locus_tag="VI359_05160"
     CDS             complement(6661..7011)
                     /locus_tag="VI359_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973673.1"
                     /translation="MLGVERVYIARDMQERKTTRLKAKLRIFFSGGGVEGEGTVLDLS
                     KTGCRVDCESDIVKDTNVEAWIYSPDYDWPLKIDRAVVRWVSPKVFGLEFLEILPAQK
                     ERLRLVLNEKKLGA"
     gene            7090..7503
                     /locus_tag="VI359_05165"
     CDS             7090..7503
                     /locus_tag="VI359_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973674.1"
                     /translation="MSEIIKPSIQAFLVCDQVIEDSVTKKKSLIGLFTHLQAVSFPFQ
                     HQQMGLYFCLTDAEGTYHFDIDLVYLNSEQLVCRATLPNISIGDRLQISDFGINIPSL
                     IFPAPGRYEFRLRMEGHLIAQKDFHVMQLPTHQTA"
     gene            7631..9325
                     /locus_tag="VI359_05170"
     CDS             7631..9325
                     /locus_tag="VI359_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY6973675.1"
                     /translation="MSETSGASNFIRDIVASDHAAGRHGGRVVTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYPGGVCHLRFDDTNPTTEDPEYVQAIQDDVRWLGFDWHGKLFHAS
                     DYFEQLYDYAIRLITKGKAYVDSLTADQIREHRGTLTEPGKNSPYRDRSVAENLDLFQ
                     RMRAGEFSDGTHVLRAKIDMASPNINLRDPVLYRIRHAAHYRTGTSWCLYPAYDYAHP
                     LSDAIEGITHSLCTLEFEDHRPLYDWVVEQAESTHRPQQIEFARLNITHTVMSKRRLL
                     ELVGKKLVAGWDDPRLPTIKGLRRRGYTPEAIRAFCEHIGVAKRDAVVEMQLLEHFIR
                     EDLNKRVQRVMAVLRPLKLVIENYPEGQAEQLDAINNPEDLSAGTRKVPFSRILYIEQ
                     DDFREDPPKQFFRLAPGREVRLRYAYIIKCVGVTKDPQTGEIAELRCTYDPETKSGSS
                     QEQRKVKATIHWVSAMHAVEAEVRLYNPLFTADLAKVPPDHDWTTYLNPQSLERITGC
                     RIEPSLKQAVPGSRYQFERLGYFCVDSDSSRGTTVFNRTVALKDAWARIDKSQQTR"
     gene            9463..10119
                     /locus_tag="VI359_05175"
     CDS             9463..10119
                     /locus_tag="VI359_05175"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY6973676.1"
                     /translation="MRRFGLAINCWTMLVLTAVTVFFMMAGSPSPVAAGGGATDAVQG
                     TIANVMRVLDDERLKGPEQVEERRHEIEEIIRQRVDYEEMAKRTLGTQWSSLTHHEQG
                     EFVDLFVQLLRDTFAGRISERSDEQVLYLSEQREDGFAEVKTQLKGRKIDTPIDFRLI
                     HRSGDWRVYDVVIDGASIVSNYRSQFTSIIREVSYGGLVKKMRQKAVAVKVFEKNPSQ
                     "
BASE COUNT         2354 a         3020 c         2762 g         2238 t
ORIGIN      
        1 cggcttgtga tacttgatgt agatggcctt gtttccccac ggaatgacct gaccatcgcg
       61 agcaatgacg tcaccgacgg caatctggtc ccccagtttg gcagggcggc cgttgatagt
      121 gacgcgaccg ccttcaatca gtcgatcggc ttcccgacga gagcagatcc cgtgctccgt
      181 gaagaattta ttaatgcgga gtgtttcagc cacaaggagg acaaacctgt tcagtggact
      241 cgacggcccg ctcgaattct ccctaagatg cgatagatca acctggcgat agaaaactgc
      301 ggcgcgacga acccttcaat cggggcgatt tcgctgacgt ccatcccgag aattccaggg
      361 ccatttgcca aggcagttac gaagttcagt gtgtcgtacc agcctaatcc accaggttca
      421 ggagttccca gcgcgggcac gagcgacgcg tcgagcccat cgcaatcaaa ggtaagatat
      481 accggccctc ggcaagcttt gacgacgtca ggaatccatt tggttgcccg gccttcgtac
      541 ggccctgatg gatcgagcag agtcgcagca aagaatgtcg agatgcaatc ggtgctctta
      601 atgcgttcga tttcttccgg acagatcgaa cgaatgccga cctgcaccaa tggtaaacca
      661 tcgtcgacga cacgagccat aacactggca tggctatagg gattgccttg atacgccttc
      721 ctcaaatctc cgtgggcatc gatttgcacc acgcacaggt tgggataccg acgagcatgc
      781 gcgcggatag cacccagcgc gccggtatgt tcaccggtga gggtaacgag aaaccgtccg
      841 gtgttcacat gcggacgtac gaaggcttca atagcatcga ccgcggggcc gtcgatcttt
      901 ccgtcgagct ccaacgccgc cgcggtcgca atcccgcccc attcacgata gggctcacat
      961 tgaagctgtt catcgtaaaa ctcgacttga tgagacgctt cgatgatggc cgacggccct
     1021 tgatcggacc ccagaatata actcgacgtg tgttcgtaag gcgcggggag cacgtataca
     1081 ccagcccgct cgggatggca ccagggctct tcaagaccta aaaaattgtg gcctgcgccg
     1141 agccagccgg caggaagggt catagcctgc tctgttgcaa ccgcgacggg aagctatggg
     1201 cgtgacgatc tcgatcgcaa gaccagtcgt cgaaccccgt actactagcg gcgacgaacc
     1261 ttcttgggaa ggttttcaag tggaataaag accgccaacg cgaccacggt ggtccaacga
     1321 tttggcttcc cgatagcgga ctgggtaatg tttaacgtac ggacgatttt cccgcccatc
     1381 ttaaagacct gttcccgctc tttccaggcg atgttggagt caaactcgat ccccaacgtc
     1441 gtcgcgagca tttgtgcggc caaatcctcc gtatactctc ccgactcttc atcggtctct
     1501 ccataggcgt ggtgctccga cagatagccg taggtgtgac gatccgtggg aatcgcgagg
     1561 ccgatggagg ccgatatcaa tcgattacgc tcgttcgttt ccgagcgagc catgacgcaa
     1621 tgggtgatct cgccagggtt caagagtgtt tcgcctcgtt tgcggggaat gattttgcag
     1681 ttcggcggga gaatcgagga cacgctgacg aggttgcagt aggcgacccc ggcgctgcgc
     1741 aacgcttgct cgaacgaggc gagcttttcg cgatgcacgc ccacgcctct actcagaaac
     1801 atatgcgtcg gtaccatagt cctcttctcc cttgtacaga ttcacagcgt gggcgctcgt
     1861 tcctcaccca gagcttaagc acagggtcgc tgttgtacca agaatgacgc cgcttcgcaa
     1921 tatccctgct cgcttttttc agctaggcaa caccgggcga cttcaaattc agcacgagga
     1981 ggcgaggctc ggtcatctcc tcgattgccg ccttgacccc ctctcgccca cttccggaat
     2041 ctttcacgcc gccatagggc atatgatccg cccggaaggt cggaatttcg ttggccagca
     2101 ccgctccgac ttccagatgc cggaaggcgt gaaacacttt gttgatgtct tgcgtgaaga
     2161 cgcctgcttg cagcccatag tcagaatgat tcaacgctgc aatcgcctcg tcaaactggc
     2221 gataggacgt gagcgtcacg acaggaccga acacttcctg acacgacacg ttcattgtcg
     2281 gtgtcacatt ggagagtacc gttgcctcaa ggaccgagcc catccgcttg ccgcccaaga
     2341 gaactcgcgc tccttctgag acggcctctc cgatccagcc ttccactcga gcggctgcgg
     2401 acgggttgat caaggggccc acgactgtgg cttcatcgtt aggatcgcca gccttcagac
     2461 gcgcgacctg gagcagcagc ttcgtcgtaa agagatccgc gaccgaatgg tgtacgaaaa
     2521 tccgctggac ggagatgcag gtttggccgg catagccgaa tcctcctgtc gcacagcgtt
     2581 gcgcggcggc gtccaagtcc gcgtccgcct ccacaatgac gccggcatta cccccgagtt
     2641 caagtacgac cttcttcttt ccgcactttg ccttcaacat ccatcccacc gcggcgctgc
     2701 cggtaaaact taacagcttg aagcgaggat cgataaccaa gtgttcagcg atggtgttgt
     2761 cgcacggcac cacattaagc ccgccaggcg ggacccctgc ttccatggca acttcgccga
     2821 gcagaagcgc ggtcaagggt gtctgtgggg cgggcttgat gagaatcgga ttcccggctg
     2881 cgagggccgg cgccactttg tgcgccacga gattgagcgg gaagttgaac ggagtaattc
     2941 cgagcaccgg tccgatcgga aatcgccgga ggatgccgag atgcccgtcg gtcccgggag
     3001 tccaatcgag tggaatcacc tcgcctggaa tccgctttgc ctcttctgcc gcaacggtga
     3061 aggtttgaat ggcacgattg acttcccgtc tggcatccgt gattggtttt cccgcctcgg
     3121 cggtcatcag acgggcgaat tcttcccgcc gatcgtacag cgccccggcg atcttttgga
     3181 ggagatgata gcgtgcgtga gaaggcaacg acgccatggc cgacgccgcg tcgacggttg
     3241 actgaatcgc ggcctcggca tccgcctggc tggcttgcga gacctcggca atcacctgac
     3301 ccgtgaacgg atcattcaca cggacgacgc tgtcgccgta tcgccactgg ccgccgatta
     3361 agaatggacg tggtccctgc aacggagacg ctccgccaag cacgaagagt tagtccgcgg
     3421 actcaagtga ggacgcagga gtcggttcgg ggctggaagt cgccgctcgc tgctcctcgg
     3481 aggcagctct cgcgatcaac tcctctgtgc cccaatcacg aatcgcctca agagaaaagg
     3541 cttcgtacgt cgaaactccg tgacatgtgg gacaatccgg catggggacc ttgcgataga
     3601 agacttcgcc caggcaggac atgcagaccc aaaatgcggg ctccccttct cgttgagaaa
     3661 cgggccagaa cgattgtgat gcagacataa tgatggttcc tacgtgatgc ggatcgtgta
     3721 atgtcccgcc gccgaatgct cagacgatcc gctgagtgcg gggcggcgct gcctaggagc
     3781 gcggtgcact gcgcaacaaa cgctcaatct cctcggcaaa atcgtcaaat ggaaacgcgc
     3841 cgggaatggc cactgccggt tctttttccg tcgcgcctcc gactgtatgc atcaaaataa
     3901 acccgggggt cccatgaaac ccccagcggt tggcctcctg acgatcttgg aaaatagact
     3961 caagataccg ccgttcctca aagcactgag caaaaaccac ttgatcaagg ccgatagtcc
     4021 tggcgtaact tcgaaaggcc tcggagtcca agcgccttcc ttcgccaaac aatcggtcat
     4081 gcatggccca atagcggccc tgtgcccccg cgcaccgtgc cgccacggcc gcctcgactc
     4141 ccaccccccg atccgctcga gggtaatcgc ggtacacgaa tcgcagcttg ccggtttcga
     4201 cgtacttcgc ctgcagtttc ggccaggtgt cgcggaaaaa cttcagacaa tagccacagg
     4261 taaagtccga atattcgatc aaggtgaccg gagcatctac gcggccccgg atgcgcccat
     4321 cgtccgtgac ctcaccgatt cctgcaactg cgagcggatc gagcgtcagg cttatcagaa
     4381 acaagaccag gccgatgatt cgtgtgagac cgaccaccat catgtacgct tggactgttt
     4441 ggcctttttc ctctccagca acccgaccat cagtatcggc cacacaactg tggcatcgct
     4501 caacacttcg gcaaagcggc caccctcttc gggcggaaca aacttccccc acgaaatgcc
     4561 ttcttgatac gtgcatccgc ttaacccgcc ccaatagtcc ggctccggac agattcgcac
     4621 accatattgg aaccgaggcg gcttcacatt cagccccagc cgcatgttgg cgatttcgat
     4681 gtagggtcct acttgttgtg cccaattgcg tggtacgccc cccccgatcg taaaaatgcc
     4741 cagtcgcttc gcggatgtca cgacattggt atagctattg aggtcgagat aggggttgaa
     4801 ggaaggacag gagcgattga tgaaacggag cacctcaagg tcattgtccc ctttcgcctg
     4861 cgaccgtgcc cgatcgattt cccgacccat agcccatgtg ccgacatcca gacccatttc
     4921 cgaatccgtg aaggcaggaa tatacaccgg gactttttta acatatgcac tcttgaggat
     4981 tccgtcgccc tcaaactcct cggccagggt tttcccaagt tcgcgcgtca gaatttcaga
     5041 cgaaagcggc tgatcgtggt tgatccgctt catcgtctgc gcgacgacat gctccacgta
     5101 gtttaggttc gcttccattt ccagtgtgtc gtacacacga ttgtagccct tccggaacag
     5161 ttcctcgtcg ctcatcgacg ggcgatggcg ataatgcgtt ttcccgacag attcgctgag
     5221 cccatgcgcg atcaacgcgc ccgtcgaaac aataccttgt accatgccgt ggtcgatcat
     5281 agtgctgaca atcttgccca tcttggcgat tgtcatcgcc ccggacagcg tcaggatgac
     5341 cgaacagtct gggtcctcga tcatcgacca cagcacttcg aaagcttcgc ccaatcggcg
     5401 gccaccaaag gctgttttgc gcatggcggt caggaggtct tcgaaggacc ggatcttttc
     5461 tggatcaagc ggctcgagcg gctcgagtcc gtcttttgca ccgtcgtgga actcacgtgc
     5521 agccattgaa gagtctaccc gggtcagccc tgaacaaatt aagaaaggcg ccttcgttat
     5581 acacaaccaa tgataggggc gtcaacgaag tttgggcagt ggagaagcac gcggcgtgca
     5641 tacacacggt ttaacgaaaa agggatccct ggaggtgtgt agtcgccgac aacaatcacg
     5701 atagcgcgat agtgagtcgg cggcacatac gaagtgccgt ttcaaattac ggggatctct
     5761 ggagaggtta tccgcccgcg ccagattacg atgaggcgat gaagagcggg tggcacgcgc
     5821 aagcaaggat gtggtggtcc caacgggatt cgaacccgtg tttgggcctt gagagggccc
     5881 cgtcctaggc caggctagac gatgggacca gcgaggcgta cagcaaacgg agcggcacag
     5941 tagcataggg tcttttgcct tttcaattgt tctggattct ttgaaagctt tccgatgaat
     6001 gggctctttc tttcccgact tgacacagac acaaacatgg cctacctttc gacaacttcg
     6061 taccccgcgc cgcagggaaa gctctgttag agaccaatca tgaacgagca actccaaacc
     6121 aacaaaatag aaaacggaga ttccgctgaa ctcggcgaag aagcgaaacc ggagaccggg
     6181 ctcgagctgc atatgggctc gaatgtcttc cgcaacatca atggggtagt gaaacttcag
     6241 ggcaaggagc agatgttttg ggaagttgtg cccgacctcg agaccctgct cctcaccatg
     6301 gacttgtacg atgacaaagg cgtccgagtg ggccacattc gccgaaacgt catcaagagt
     6361 ggtgagatcg gacgctttac cgtcactgcc aatttacacg ccaatgctcg accggacagc
     6421 gctccatcgg tcactgtttc tgatcgatcg aacggacaga ccgtggtcga agcgttcctg
     6481 gtccaaggac gaaaaatccg tattgcggcg gggagcttct atacccataa gggtgaattg
     6541 gtccaaatca gtccgcacta ctgtcggatc gggaccggga ggacgctctt cggtgatgtg
     6601 gtggagagcc gcgggggcaa cgcgttgatc gattgatctc ttatcggacg ctacaaatcg
     6661 ttacgcacct aacttcttct cattcaacac cagccgaagc cgttccttct gggccggaag
     6721 gatctccaaa aactccaggc cgaacacctt cggtgacacc catcgaacta ctgctcgatc
     6781 aatcttgagc ggccagtcat aatcgggcga atagatccac gcctcgacat ttgtatcctt
     6841 gacaatatcg ctttcgcaat ccacgcgaca gccggttttg gagagatcca atactgtacc
     6901 ttctccctca acgccgccgc ccgaaaagaa gatgcgaagc ttggctttca gacgagtggt
     6961 ttttcgttcc tgcatgtccc gagcaatata gacccgttca actcctaaca ccctctgttc
     7021 ttttgtgcca tatctggaga aaatacggaa gccttttgac aataggaaga tccaccgtag
     7081 aatactgtca tgtccgaaat cattaagcca agcattcaag cgttcttggt ttgcgaccag
     7141 gtcattgaag acagcgtcac caagaaaaag agtctcatcg gacttttcac ccacttgcaa
     7201 gccgtcagct tccccttcca acatcaacaa atgggcctct atttctgcct gaccgacgca
     7261 gaagggacct atcacttcga catcgacctc gtgtatctga atagtgaaca gttggtctgc
     7321 cgcgctacgt taccaaatat ctccatcggc gaccgcctgc aaatttcgga ttttggaatc
     7381 aacattccct cgttgatctt ccccgcccca ggacgttatg aattccgtct tcgcatggag
     7441 ggacacctca tcgcccaaaa agacttccat gtgatgcagc tgccgaccca ccaaaccgcc
     7501 tagggtcacc ggaggtggac aggaccctcc tacttgcccg aggccttctc ccctacgtcg
     7561 ttctgtggta gaagagtccc acacacctga ttcgtatccg ttccatctgc gcaggagtca
     7621 ggaaagaatc gtgtctgaaa ccagcggcgc ttcgaatttc attcgcgaca tcgtggcgtc
     7681 cgatcatgct gctgggcgac acggcggacg tgttgtgaca cggttccccc ccgagccgaa
     7741 tggttatctg catattggcc atgcgaaatc catttgcctc aacttcggca tcgctcagga
     7801 ttatcccggc ggggtgtgtc atctccggtt cgacgataca aacccgacca cagaagatcc
     7861 ggaatatgtg caggcgatcc aagacgatgt gcgctggttg gggttcgatt ggcacggaaa
     7921 gctgttccat gcttctgact actttgagca gctctatgac tacgcgattc gcttgattac
     7981 caaaggcaag gcttatgttg acagcttgac ggcggaccag attcgcgagc accgaggaac
     8041 attaaccgag cccggcaaga acagccccta tcgcgaccgg tcggtcgctg aaaatctcga
     8101 tctgtttcag cgcatgcgtg ctggcgaatt ttctgatgga acccacgttc ttcgagccaa
     8161 aatcgacatg gcctcgccga atatcaatct gcgtgatccg gtcctctatc gtatccgaca
     8221 tgccgctcac tatcggaccg gcacctcttg gtgtctctat ccagcctacg actatgctca
     8281 tccgctatcc gatgcaatcg aagggattac gcattctctc tgtacattgg aattcgaaga
     8341 ccatcgtccg ttatacgatt gggtggtcga acaggccgag tccacacatc ggccacagca
     8401 gatcgaattt gcccggttaa atatcaccca cacggtgatg agcaagcgca ggctgctgga
     8461 attggtcggg aaaaaacttg tggctggatg ggatgatccg cgcctcccca caatcaaggg
     8521 cttgcggcga cgcgggtata ctcccgaagc catccgggct ttctgcgaac acatcggcgt
     8581 agccaaacga gatgccgtcg tcgaaatgca gcttctcgaa cattttattc gagaggatct
     8641 gaacaaacga gtccaacggg tcatggccgt gttgcgccct ctgaagctcg tgatcgaaaa
     8701 ctatcccgaa gggcaggctg aacaactcga cgcgattaat aatcctgaag atctgtcggc
     8761 cgggaccagg aaagttccat tctcacgtat cctctatatc gaacaagacg atttccgaga
     8821 agatccgccc aaacaattct ttcgcctcgc tccaggccgc gaagtccggc tacggtacgc
     8881 ttacatcatc aaatgcgtcg gggtcacgaa agacccgcaa accggcgaga tagccgaact
     8941 ccgttgcacg tatgatccgg aaacaaagag cggctcgtcg caagaacagc gcaaggtcaa
     9001 agcgacgatt cattgggtct ccgccatgca cgctgttgaa gcggaagttc ggctctataa
     9061 cccgctcttc accgccgatc tggccaaagt cccgcccgat cacgactgga cgacatacct
     9121 gaatccacag tcattggaac ggatcaccgg ctgccgtatc gagcccagtt tgaaacaggc
     9181 tgtccccggg tcccgatacc agttcgaacg tctcggatac ttctgtgtgg actcggattc
     9241 atctcgcgga acaacggtgt ttaaccgtac ggtcgccctc aaggatgcgt gggcgagaat
     9301 cgacaaatct cagcagaccc gctagtcctc tgtcgccctg ccggtttggc accaacaaat
     9361 ctttcctctt tccgccacgt tgcactggct ttacagactc cacttccctg ctacgcttag
     9421 atgagcacgc caagagccct caataggagc cgatttccag ccatgagacg attcggtcta
     9481 gcgatcaatt gttggacgat gcttgtgctt actgcagtaa ccgtcttctt catgatggcc
     9541 ggttcacctt cgcctgtcgc cgctggaggg ggagccactg acgcagtgca aggcaccatt
     9601 gcgaatgtga tgcgtgtact cgatgacgag aggttgaaag gtcccgagca ggtcgaagag
     9661 cgccggcatg aaatcgaaga gatcatccgg cagcgtgtgg attatgagga aatggctaaa
     9721 cgaaccttgg ggacacaatg gtcgtcgtta actcaccatg agcaaggtga attcgtcgac
     9781 ctatttgtcc aactcctcag ggacacgttt gctggacgga tttccgagcg ttccgatgaa
     9841 caggtactct atctcagtga acagcgggag gacggattcg cggaagtcaa gactcaactg
     9901 aaaggacgga agatcgatac tcccatcgat tttcgcctca ttcaccgctc cggcgattgg
     9961 cgggtctatg atgtcgtcat cgacggggcg agcattgtga gcaattatcg gtctcaattc
    10021 accagtatca tccgcgaggt gtcgtacgga ggattggtga agaaaatgcg gcagaaggcc
    10081 gtcgccgtca aagtcttcga aaagaacccg tctcagtagt tttctctttc caattgcctt
    10141 gtcccacttc catgcttttt ctttaccgca gactatgatt tgctagacta cttctatcga
    10201 agtgaggcca cgcctcgcgc ccgcaacgga aggagacatc atgattgaat gtcgacagta
    10261 tccgcgcgtc ccagttgatc tccaagtctt cttttccacc acagggaata cggatattcg
    10321 agaagggacg atgttcgacc tctccctggg ggggtgcgca gtgaccacta tgac
//
LOCUS       DATDNW010000207         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_26147,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000207 DATDNW010000000
VERSION     DATDNW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26147"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VI359_05180"
     CDS             <1..493
                     /locus_tag="VI359_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HEY6973677.1"
                     /translation="YPNDFATWVVRHAQDRVLGERLGVVDPFDFDDIEAVRAELVGII
                     GDHLDRARAPVSSRGSEPFEFIRSHVIEVDLYIEVATLQEFRDELSHVEIGAVYNHLC
                     EARMRKRRPSGDFADWLSSDAGLGLSELGAQTVKVARIGLSLEDMRARIVNLCDQALK
                     ATA"
     gene            520..1728
                     /locus_tag="VI359_05185"
     CDS             520..1728
                     /locus_tag="VI359_05185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6973678.1"
                     /translation="MAPMLAEYTDVAPTGTVEFLRRLGEMAEGRTMLHVNSTRYGGGV
                     AEILHRLLPLMDELGVKTRWEVLTGTDLFYRTTKSFHNALQGTKQRLTKEMFDAYIEC
                     NRENAKRLNLDADVALIHDPQPAPLIDSRPSGAKWMWRCHIDLSTPQRSVWQFLRPYI
                     IRYDAAVFSLPKFTQRLPIPQFLVYPSIDPFSDKNRELPDEQVGAVLDRLEIPRDKPI
                     LLQVSRFDRFKDPVGVVEAYRIVKKSHDCRLVLAGGSADDDPEGAEVLAEVREVVGVD
                     PDVHLLLLPPTANIEINALQRAAAVVLQKSTREGFGLTVTEAMWKGKAVIGGETGGIT
                     AQIIHGKTGYTVNSPEGAAFYIRRLFNDPDLMGAIGRRAKEYARHRFLITRQLQDYLG
                     VMIHLSRQAA"
     gene            1754..3217
                     /locus_tag="VI359_05190"
     CDS             1754..3217
                     /locus_tag="VI359_05190"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY6973679.1"
                     /translation="MLDVHRLIAVSNREPYEHHWENGQLVCKRTDGGLTSALDPVLRR
                     NGGTWVAWGSGPADRDVVGPDMSVEVPPESPAYRLRRVWLSQDEMQDGYRGYANQVLW
                     PLCHITLDRITYLKAFWPAYRSTNLCFADAVLEELDREPGPVWIHDFHLALLPALVKA
                     ARPTVPVSVFWHIPWPGPDVWRILPERREILRGLLAADLLVFQTPSHAEAFLRCAHDF
                     LGGIIHASRDQVLYNGRDTRVMAHPISVDFRLFSDHARSAAVNQTEAVLRQRFNLRAG
                     VRLGLGVDRLDYTKGLLKRLWALDAFFSQFPAFRGKFMFLQIAIPTRGELDTYQRYRD
                     LIRETVSEINQRHAAEYTSETNGNSNWTPIELYEGRIGFDELVALYRMADLALVSSVY
                     DGMNLVAKEYVSAQVDERGVLLISQMAGASEELSGAIVINPYAIEGVAEAVKEALEMP
                     QDERRRRMRRMRSYLAAHDVHAWVDRCLNEALTGIHH"
     gene            3222..4055
                     /gene="otsB"
                     /locus_tag="VI359_05195"
     CDS             3222..4055
                     /gene="otsB"
                     /locus_tag="VI359_05195"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY6973680.1"
                     /translation="MSPAGILSVRSQLESIGLSRLVLFLDFDGTMVPIAARPQHVRMS
                     KALKRTLGRLGHRIPIAVVSGRPISDLRRVMGLRHIHYIGMHGFSYATPGKPPTWLAP
                     RPGRRIVRQWKAALEAVARKVQGAWVEDKGIAVALHDRQVAPQDRRRLRRQALGALQD
                     WLREKQIRLLSGKHIIEVLSVTRCDKGSAVMRLLEQPWARSRVPVYFGDDTTDIPALE
                     LVARSGIAVQVGSIPHAPKTYLRLANSSEVEQLLREMANRLDDTVFVERQRLANSSCP
                     A"
BASE COUNT          864 a         1095 c         1248 g          912 t
ORIGIN      
        1 atatccgaac gactttgcca cctgggtggt gcgacatgcg caagaccgtg ttctcggcga
       61 acgattggga gtcgtggacc cgttcgactt cgatgacatc gaggctgttc gggctgagct
      121 ggtcggaatc atcggcgatc accttgatcg tgccagagcg ccggtctcga gcagggggtc
      181 cgaaccgttt gaattcattc gttcgcacgt catcgaagtg gatttgtaca tcgaggtggc
      241 cacgcttcaa gaatttcgtg atgaactaag ccacgtcgaa attggcgcgg tctataacca
      301 cctctgcgaa gcgcgcatgc ggaagcgtcg acccagcggg gatttcgctg actggttgtc
      361 ctcggatgcg ggactgggct tgtcagaatt gggcgcacag accgtcaaag tggcccgcat
      421 cgggttgagc ttggaagaca tgcgcgcgcg tatcgtcaac ctctgtgatc aggccttgaa
      481 ggcaacagct taatcaggtc aaccagggag aaagacggaa tggcaccgat gttagctgag
      541 tacaccgacg tcgctccgac cggaacagta gagttcctca gacgactggg tgagatggct
      601 gaaggcagga cgatgctcca tgttaattca acccgctatg gcggcggcgt cgcggaaatt
      661 ctgcatcgat tgcttccgct catggatgag ctgggagtga agaccagatg ggaagtgctg
      721 accggcaccg atctctttta ccgcacgacc aagagctttc acaacgcact gcaggggacg
      781 aagcagcgac tgaccaaaga gatgttcgac gcctacattg aatgcaatcg cgagaacgcc
      841 aagcggttga atttggatgc cgacgtcgct ctgatccatg atcctcaacc ggctccgctc
      901 atagacagcc gtccatctgg cgcaaagtgg atgtggcgct gccacattga tttgtcgacg
      961 ccgcaacgat ccgtctggca attcctacgt ccctacatta ttcgctacga cgccgctgtt
     1021 ttctccttgc cgaagttcac ccagcgcctc ccgatccctc agtttctcgt ctacccgtcg
     1081 atcgatccgt tcagtgacaa gaatcgcgag ctcccagacg agcaggttgg agccgtgttg
     1141 gaccggcttg agattccgag ggataaacct attctgttgc aagtctcacg gttcgaccgt
     1201 tttaaagatc cggtcggagt ggtcgaagcc tatcgaatcg taaaaaagag tcacgactgc
     1261 cgattagtgc tggcaggcgg gagtgccgac gatgatccgg aaggtgcgga agtcctggca
     1321 gaggtgcgtg aagttgttgg tgtcgacccg gacgtccacc tcttgttgct ccccccaacg
     1381 gccaatatcg agatcaatgc gctccaacgg gccgcggcgg ttgtcctaca aaagtccacg
     1441 cgtgaggggt tcgggctcac ggtcaccgaa gccatgtgga agggcaaagc cgtgatcgga
     1501 ggggagacag gcgggattac ggctcagatc attcacggaa aaacaggata caccgtcaat
     1561 tctccggaag gggcggcctt ctatattaga cgcctcttca acgatcctga cctcatgggc
     1621 gccattggac ggcgagccaa ggaatatgcc aggcaccggt ttttgattac cagacagctt
     1681 caggactacc tcggggtgat gattcatctg tcccgtcagg ctgcgtgaaa agagaacacg
     1741 ggaggtttta caaatgttgg atgtgcatcg actcatcgcc gtctcgaatc gtgaaccgta
     1801 cgaacatcac tgggagaacg gtcagctcgt gtgcaagcgg acagacggag gactgacgtc
     1861 ggccttggat ccggtgcttc gccgaaatgg ggggacatgg gttgcctggg gaagcggacc
     1921 ggctgatcgc gacgtcgtgg gacctgacat gtccgtcgag gtgccgcctg aatcgcctgc
     1981 ctatcggttg cggcgtgtct ggctgagtca ggacgaaatg caggacgggt accgtggtta
     2041 tgccaatcag gtgctgtggc cgctgtgtca tatcacgttg gaccgcatta cttatctgaa
     2101 ggcattttgg cccgcgtacc ggtcgaccaa tttgtgtttc gccgatgccg ttttggaaga
     2161 gttggatcga gaaccggggc ctgtgtggat ccacgatttt cacctggctc ttctccctgc
     2221 gctggtgaag gctgcgcggc caaccgtacc ggtgtctgtc ttctggcaca tcccatggcc
     2281 aggtccggac gtatggcgca ttctccctga acgccgggaa atcctgcgcg ggctgttggc
     2341 ggctgatctg ctcgtttttc aaacgcccag tcatgctgag gcatttcttc gctgtgccca
     2401 cgatttcctc gggggcatca ttcatgcctc tcgagatcaa gtgctttata acggtcgtga
     2461 taccagggtg atggcccatc ctatcagtgt ggattttcgg ctattctctg accatgcgcg
     2521 cagtgcggct gtgaatcaga ccgaagcggt cttgcgacaa cgatttaatc tgcgcgcggg
     2581 agtacgactc gggcttggcg tcgaccgatt ggattatacg aagggtttgc tgaagcgtct
     2641 gtgggcgctc gatgcgtttt tctcacagtt cccggcattc cgtgggaaat ttatgtttct
     2701 ccaaatcgcg attccaacgc gcggggaact cgatacgtat caacggtatc gcgacctcat
     2761 ccgggaaacg gtgtcagaga taaaccaacg tcatgcggcc gaatacacct ctgagacaaa
     2821 cgggaatagc aactggactc ccattgagtt atatgaagga cgaatcggct tcgacgaact
     2881 cgtggccttg taccggatgg ccgacttggc cctggtcagc tccgtgtacg acgggatgaa
     2941 tcttgtggcc aaagaatacg tgtccgccca agtcgacgag cgcggtgtct tactgatcag
     3001 tcagatggcc ggagcatcag aagaattgtc tggggcgatc gtgatcaacc cctatgcaat
     3061 cgaaggcgtg gcggaagccg ttaaagaggc gttagagatg ccgcaagatg aacgtcgacg
     3121 gagaatgcgt cgaatgcggt cctaccttgc ggcgcatgac gtccatgcct gggtggatcg
     3181 gtgcttgaat gaggcgttga cgggtataca ccattgaagt gatgtcgccg gccggtatct
     3241 tatctgttcg ctcacagctt gagtcgatcg ggctatcccg cctggtgctc tttctcgatt
     3301 tcgatgggac gatggtccca atcgctgcac gaccgcagca tgtaagaatg agcaaggcgc
     3361 tgaagcgaac cctgggaagg ttggggcacc gaataccgat cgcggtggtg agcggacgtc
     3421 cgatttccga tctccgccgt gttatgggtc ttcggcatat ccattatata ggtatgcacg
     3481 gcttctctta cgcgacaccg gggaagcctc ctacatggct ggcaccacgt ccagggcgtc
     3541 gaatcgttcg ccaatggaaa gcggcgctcg aagccgtagc aagaaaagtc cagggtgcct
     3601 gggtcgaaga taaaggtatc gccgttgcac tgcacgatag acaagttgct cctcaggatc
     3661 gccggcgcct ccggcgccaa gcgctggggg cgttgcagga ctggcttcgt gagaaacaga
     3721 tccgtttgtt gagcgggaaa catatcatag aggtactttc ggttacccgc tgtgataaag
     3781 ggtccgccgt gatgcggctg ctcgagcaac cctgggcccg gtctcgagtc ccggtgtact
     3841 ttggtgatga caccaccgac ataccggctc tcgagctcgt tgctcggtcg ggcatcgccg
     3901 ttcaggttgg atcgatcccc catgcgccca agacgtacct gcgattggcg aacagcagtg
     3961 aggtagaaca gctgctgcgg gaaatggcga atcgactcga tgacacggtt tttgtggagc
     4021 gtcagcggct ggccaacagt tcctgccccg catgattcca gccgatgggc ccgatgccgg
     4081 aggctcggat catgcctggt atgtggatat caggatcgt
//
LOCUS       DATDNW010000208         3837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_641058, whole genome shotgun sequence.
ACCESSION   DATDNW010000208 DATDNW010000000
VERSION     DATDNW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641058"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VI359_05200"
     CDS             <1..504
                     /locus_tag="VI359_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973681.1"
                     /translation="QYREVIKLNPDDAVAHANLACALAEQGKMQAALLEYQEALRLNP
                     SDAEIHFAIGGLYENKNRPDLAMKEYRAAVTANPNLAPAHSALGWMLFDRGHREEAME
                     CFNHALKADPEDARAYLGVAKVYASRGKVQLAIENYTKAIKYERDPQKKNDIMNKLYK
                     EGGTWDT"
     gene            912..1274
                     /locus_tag="VI359_05205"
     CDS             912..1274
                     /locus_tag="VI359_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973682.1"
                     /translation="MADKNDTLEQDIHNVLFPNIEFMWVDYAKARDLAVDSLDKNWRN
                     AKCDVLTLWCHIKHCGDVFVTSDSNFHATTKRDNLKALGAGIVAYPKRGTAVGWQFMR
                     KMAERRRLLKQAVTKATG"
     gene            complement(1358..1627)
                     /locus_tag="VI359_05210"
     CDS             complement(1358..1627)
                     /locus_tag="VI359_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973683.1"
                     /translation="MKGLRFERIGQDRYYNVVFHMGSTYVPVSDDTVEELKAQSLLPP
                     ERFLDLLIDRIGYSSYLKDQIRTELKSSGDPVTQITVLQGAIRDL"
     gene            complement(1726..2067)
                     /locus_tag="VI359_05215"
     CDS             complement(1726..2067)
                     /locus_tag="VI359_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6973684.1"
                     /translation="MGALTLHPMKEIRVIVAGEHRAFVTDLLDRVNATGYTIIGNVSG
                     KGHHGVREAHFMFSEQESLEMIMTVVPEEKVEPILAGLRPLFERHSGVMFVGDVAVSR
                     EEYFGKKGKGA"
     gene            complement(2116..>3837)
                     /locus_tag="VI359_05220"
     CDS             complement(2116..>3837)
                     /locus_tag="VI359_05220"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEY6973685.1"
                     /translation="GPNETYGFAGFFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIP
                     RSYLDHRVSKHKARARWVRAGHTLLHDLKENVVTPYVMVESVGWFYSLPIFGKTLVPS
                     LYRRWTGWLSRLFVPSIATTLTVDKLPPAETVEMLAAEQQALVTKALQEETGLRSSYI
                     TPALVEALRERALGGPGDPGPPLIEAATQAGLSIETLNRFIETLRRQYDLNARSASRQ
                     KERITRTGFTLEEQTLTVDTALRMMGLTKNFARLVLFCAHGSTSDNNPYESALDCGAC
                     GGNEGKPNARVLAMMANNQKVRERLAKNGIEIPPDTHFLAGQMDTTTDEVHLFDLEDV
                     PPTHRADLARLQGDLKKASALTSQERCARFPDVHRTVEEPKAKTHVRRRSADWSQVRP
                     EWGLSSNTAFVIGRRELTKGLNLAGRVFLHSYDYREDPTNRLLEVLLTAPQVVAQWIN
                     MEHYFSAVDNDVYGSGSKVYHNVVGRLGIMSGPWSDLRLGLARQTVMNGDMPYHEPMR
                     LLTIIEAPRDRIEKLIARHEVLRHYYHNEWVHLVALDPEDQVWYRYRPNGEWRPIMEE
                     AVKNASN"
BASE COUNT          827 a         1082 c         1107 g          821 t
ORIGIN      
        1 cagtaccgcg aagtgatcaa actcaaccca gacgacgccg tcgcccatgc gaacttggcc
       61 tgtgcgctcg cggagcaagg gaagatgcag gccgcgctct tagaatacca agaagcgctg
      121 cgcctgaatc cgagcgatgc cgaaatacac tttgccatcg gcgggttgta cgagaacaag
      181 aatcgtcccg atttggcgat gaaagagtat cgtgcggcgg tcacggccaa cccgaacctg
      241 gcgccggccc actcggcgct cggctggatg ctcttcgaca gagggcatcg cgaagaggcg
      301 atggaatgct ttaatcacgc cctcaaggcc gatccggagg atgcgcgagc ctatctcggc
      361 gtcgccaagg tctatgcgag tcgcggcaag gtgcaattgg cgatcgagaa ctacaccaag
      421 gccatcaaat acgagcgaga cccgcagaag aagaacgaca tcatgaacaa actctataaa
      481 gaaggcggga cgtgggatac gtgaggtgtg cagaatgccg ggcttggaag gagtatgtgt
      541 agcgggcgct actcgtgcaa cgcgcgtacg gagtttgttt ttggctagat aatcagagta
      601 gacaaatcag tttgcataaa taggtagctg gcaccaaccg catgcgtttt actctggaca
      661 cgaagtgcct cattgacgtc gaggaaggac gaccaagcgc atcgtttatc agagttcttg
      721 tctcacttca tggaaagaac ggaatcacag ttgcaatttc ctcaataggg gcatctgaac
      781 ggcaacgggc cggtggatat gctcagaatt tctctgagtt ccaagcgaag ttaaaagcaa
      841 ttggtttcga gtacttggag catcttccgc ccttggcata ctggaatatc tgcttttttg
      901 accattgcgt catggcggac aagaacgaca cgctggagca agatatccac aatgtgctgt
      961 tcccgaacat tgaattcatg tgggtcgact atgcaaaggc tcgggatctc gcagtagatt
     1021 ccttagacaa gaattggcga aacgccaaat gcgatgtctt gacgctctgg tgtcatatca
     1081 aacattgcgg cgacgttttc gtgaccagtg acagcaattt tcatgctacg accaaacgag
     1141 acaatctgaa agcattaggt gcgggaatcg tcgcataccc taagagaggc actgcggttg
     1201 gctggcaatt tatgaggaag atggcggagc gtcgacggtt gctcaaacag gcggtcacca
     1261 aggccacagg ttagaaaata ccggaggcgt accctctggg gtacgttgag gatttctcga
     1321 gccgagaacg aagttgggcg cctgtttcag caaccgatta gaggtcgcgg atggcgccct
     1381 ggaggacggt aatctgcgtg accggatcgc ccgaagattt cagttcggtg cgaatctgat
     1441 ccttgaggta ggacgaatac ccgatccgat cgatgagcag atcgaggaat cgctcgggcg
     1501 gcagcagact ctgagccttt aattcctcta cggtgtcgtc gctcaccggc acataggtgc
     1561 tgcccatgtg gaagacgaca ttgtagtagc gatcctggcc gatacgctcg aaccgtaaac
     1621 ccttcaccga tccctccgtc gtggaaccgc ctcagagggg cccattctag acaagttctg
     1681 gtcgtgaaga caagagcggg gcgttgcatc accgacgatg gctggtcagg ctcctttgcc
     1741 cttcttgcca aagtattcct cccggctcac agccacgtcg ccgacgaaca tgacaccgga
     1801 gtgccgttcg aagagcggtc gcaggccggc caggatcggc tcgaccttct cttccggcac
     1861 gacggtcatg atcatctcga ggctttcctg ctcgctgaac atgaaatggg cctcgcgcac
     1921 accgtggtga cctttgccgg acacgttccc gataatcgtg tagccggtgg cattgacccg
     1981 gtcgagcaaa tcggtcacga aggcccggtg ttctcccgcc acaatcacgc gaatttcctt
     2041 catcggatgt aaagttaagg cgcccatcga cgaactcctt tcctcctagc cggcgacttc
     2101 taaactgctt gcgatctaat tagatgcgtt tttaacggcc tcctccatga tcggcctcca
     2161 ctcaccattg gggcgatacc gataccatac ttgatcctcg gggtcgaggg cgacgagatg
     2221 gacccactcg ttgtgatagt aatgccgcaa gacttcatgc cgtgcgatca gtttttcgat
     2281 ccgatcccgc ggcgcctcga tgatggtcaa cagccgcatc ggttcatggt atggcatgtc
     2341 gccgttcatg acggtttgcc tggccaatcc aagccggaga tcgctccagg gtcccgacat
     2401 gatgccgagg cgtccgacga cattgtgata gaccttgctc ccactgccgt acacgtcgtt
     2461 gtcgacagcg gaaaaatagt gttccatgtt gatccactgc gccacgactt gcggggcggt
     2521 cagcaggact tcgagtaggc gattggtcgg atcttcgcga tagtcgtagg aatggagaaa
     2581 gactcgaccc gccaggttca gtcccttcgt caattctcgc cggccgatca cgaacgctgt
     2641 attgctcgag aggccccact ctggacggac ctggctccaa tccgcgctgc gtcgtctcac
     2701 gtgcgtttta gcctttggct cctcgacggt ccggtggacg tcgggaaagc gcgcgcagcg
     2761 ttcttggctg gtcagtgccg aagctttttt gaggtctccc tgaaggcgtg ccaagtccgc
     2821 ccgatgggtc ggcggcacgt cttccaaatc gaagagatgc acctcgtcgg tcgtcgtatc
     2881 catttggccg gcgagaaaat gcgtgtcggg aggaatctca atcccgtttt tggccagccg
     2941 ttcccgcact ttctgattgt tcgccatcat cgcaaggacg cgggcattgg gcttaccctc
     3001 gttgccgcca caggcgccgc aatcaagggc ggattcatag ggattgttgt cggacgtgct
     3061 gccgtgcgcg cagaacagga cgagccgcgc gaaattcttc gtcaacccca tcatgcgcag
     3121 agccgtgtcc accgtgaggg tctgctcttc aagcgtgaat cctgtgcgcg tgattcgttc
     3181 cttctgccga gatgctgacc gcgcgttgag atcgtactgt cggcgcaacg tctcgatgaa
     3241 cctgttcaag gtctcgatcg aaagccccgc ttgagtcgcg gcctcaatca acggcggtcc
     3301 cggatcaccc ggcccaccca aggcgcgctc tcgtaaggcc tcgacgagcg ctggcgtgat
     3361 ataggagctg cgcaatccag tctcctcctg caacgccttt gtgacgagag cctgctgctc
     3421 ggctgccagc atctccacgg tctcggccgg cggcagctta tccacggtga gtgtcgtcgc
     3481 gatggaaggg acgaacaggc ggctcagcca cccggtccaa cgccgataga gagacggcac
     3541 caacgttttc ccgaagatgg gaagactgta gaaccatccc accgattcca ccatgacgta
     3601 gggcgtcacg acgttctctt tcagatcgtg cagcagcgtg tggccggccc ggacccatct
     3661 agcgcgggcc ttgtgtttcg acacgcgatg gtccaaatag ctccggggaa tttcccgcac
     3721 ttcgttcttg gcgcgcatga tgacggggaa ctgttccgtg tcatgttcct tgccccaggc
     3781 ccgatagcga atgaacgcgg cgaagaagcc ggcgaagccg taggtctcgt tcggtcc
//
LOCUS       DATDNW010000209         5572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_641266, whole genome shotgun sequence.
ACCESSION   DATDNW010000209 DATDNW010000000
VERSION     DATDNW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641266"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VI359_05225"
     CDS             <1..971
                     /locus_tag="VI359_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY6973686.1"
                     /translation="IVQQQLPGKKPDLTPLVRSRLHAVNGEAIKLDSEGEQEEKREAD
                     KEERRKSWYATREYVLTFLDQVPKDNTIVKGEWWKPAESFTKPLLSVEEEAAKHLGLD
                     VGSMVEFDIQGAIVSAEVRSIRKVEWGNFSTNFYMILSPGSFDGAPVTYVATLRVSSE
                     EEVPIQQAIVASFPNVSAIHIGDVLQNFARVLDRLSLAIRAVALFCVLAGGLVMAAAL
                     AATRYRRLYESVILKALGATRSLIARSFAAEYALLGGVAGLIGVCLGSLLSWVVLTYF
                     FELTWSLHPDVLLTGVSLTVLLTLAVGFLSTYRLLGQRPLAVLRHE"
     gene            complement(1067..2089)
                     /locus_tag="VI359_05230"
     CDS             complement(1067..2089)
                     /locus_tag="VI359_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY6973687.1"
                     /translation="MARLFVAEKGGRIKILDRNTNTLQATFLDISALVSAGSEQGLLG
                     LAFDPQYAINRRFYVSYTDVTGTSVIARYLADPGNPERALTPEDRIILTLAQPFANHN
                     GGMITFGPDGFLYLGFGDGGSGGDPQNHAQNLNDLLGKLLRIDVSQATQSPLPAYEIP
                     ATNPCIGQAGIRAEMWALGLRNPWRFGFDRQTGDLYISDVGQGDREEVNVSTRSSGGG
                     KGVNYGWNVMEGTICFPPGAACTQSGLTLPVLDYEHTGGACSITGGYAYRGSAIPALQ
                     GTYFYADFCSGFVRSFRYVSGQVTEHADWSSIRPGGNITSFGEDAQGELYLMTSQGGL
                     FRVVPN"
     gene            complement(2540..2734)
                     /locus_tag="VI359_05235"
     CDS             complement(2540..2734)
                     /locus_tag="VI359_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973688.1"
                     /translation="MNLSADAGGEIGLEVDDVEAMVITPRAKGTTVKLEPLSTPVCRM
                     AVILDPGGNAVELHQVTKER"
     gene            complement(2899..>5572)
                     /locus_tag="VI359_05240"
     CDS             complement(2899..>5572)
                     /locus_tag="VI359_05240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY6973689.1"
                     /translation="GLVLPLSPTTLIAERPKGEDMNHVSPSQAVDPYRLPRHVVPLRY
                     DLLLEPDLKTATFSGEETVMLTITQPTAEILLNAADLDIRTASLRTTDGTSHTATIEL
                     DGTLQRCRLAFTTRLAPGNWTLRLTFAGTLNDKLRGFYRSTYKDKAGTTHSIAATQFE
                     ATDARRAFPCWDEPDFKAVFATTLIVDPALTAVSNTPVASQTVENGKKIVRFSDTIKM
                     STYLVAFVVGTLNSTDPTMVGRTPIRLWAIPGKTHLARFGQDIAAASLSFFERYYDIP
                     YPGGKLDLLAIPDFASGAMENLGAITFRETALLVDQRAATHAELERVADVVAHENAHM
                     WFGDLVTMSWWNGLWLNEAFATFMEMLAVDAWKPEWKRWESFSMSRAAAFSVDGLQNT
                     RPIEYPVRAPKDAEAMFDVLTYEKGASVLRMLEQHIGPTVFRDGVRDYLRTHAYGNAD
                     TKDLWISLGQVAKQPVPELMDGWIFQPGFPLVTASVNQSSELVLIQQRFNYLDDAQSQ
                     QARGQRWHIPVQMRVVTGDKSITSRLLLEETEARFPVPREFDSLLLNEGGHGFYRVRY
                     APDLLARLLKTGLDKLAAVERFALINDAWAITVAGLMPITEYLDLAAQFTTDRDKNVW
                     ALLLDSFAFLNRLIDTDERPALESLVRQRVSPAVMDVGWTPKRGESELTGQLRSELIR
                     ALGKLGNDPATQQRAAELYAAFKTNAAAIHPDLVPAVVSTLAFTGDQARYEEFTERFR
                     TASTPQEERRYLFSLAEFRHKPLLEQTLARTLNGETRTQDAPFLISAVMGNVHGRELA
                     WAFVKNNWDQMDRLFPKQGLRRMCGGIAALATPELERDVRAFFTSRKIDLGGKTLDQY
                     LEQLRIAVNMQERDKKQLIAYLHTSRDREAEARL"
BASE COUNT         1153 a         1515 c         1650 g         1254 t
ORIGIN      
        1 ggatcgtcca acagcagctc cccggaaaga agcccgacct cacaccactg gttcggtctc
       61 gactccacgc cgtcaatgga gaagccataa aactagactc ggagggtgag caggaggaga
      121 agcgggaggc ggacaaggag gagcgacgaa agagttggta cgccacgcgt gaatacgtcc
      181 tgacctttct ggaccaggtc ccgaaggata atacgattgt gaaaggcgaa tggtggaagc
      241 cggcggagtc ctttacaaaa ccgctgttat ctgtcgaaga agaggcggcg aagcacttgg
      301 gtttagacgt cggttccatg gtcgagttcg acattcaggg cgctattgtg tcggcagagg
      361 tgcggagcat tcggaaagtc gaatggggga acttctcgac gaatttctac atgattctct
      421 cgccaggatc gttcgatggt gctccggtca cctatgtagc tacgttgcgc gtgtcgtccg
      481 aggaggaagt gccgattcaa caggcgatcg tggcctcgtt tccgaacgtc agtgccattc
      541 atatcggcga tgtcctccag aattttgcgc gggtccttga tcggctgtca ttggcgattc
      601 gtgctgttgc attgttttgt gtgctggccg gcggcctcgt catggcggca gccctcgctg
      661 cgacgcggta tcgacgcctc tatgaatcag tgattctgaa ggcactagga gcgacgcgca
      721 gccttattgc acggtccttc gctgcggagt atgcgctgct gggaggagtc gctggtttaa
      781 tcggcgtatg ccttgggagc ctgctgtcgt gggttgtgct gacctatttc ttcgagctca
      841 catggagtct ccacccagac gttctgctga ccggggtgag cttaactgtc ctgttgacct
      901 tggccgttgg atttctcagc acctaccgct tgctgggcca acgtcccctg gcagtgctga
      961 gacacgagta ggagtttcgg agggttttct caaccagaaa acttgcggaa tttctcctgc
     1021 tgtcccttcg agggtttcca tccggcttca acaaattgct cgctgattag ttggggacaa
     1081 cccgaaaaag gccgccttga cttgtcatga gatacagttc gccctgagcg tcttcaccga
     1141 aacttgttat gttgccgcca ggtctgatcg aagaccagtc ggcatgttca gttacttgac
     1201 cgctcacgta gcggaagctg cgtacaaatc cagagcagaa atcggcataa aagtaggttc
     1261 cctgaagcgc aggaatggct gaaccccggt atgcgtagcc gcccgtaatc gagcaggccc
     1321 ctccggtgtg ctcatagtcg agtactggta atgtgagacc ggattgagtg caggcagctc
     1381 caggtgggaa gcagattgtg ccttccatga cattccatcc gtagttcaca cctttccctc
     1441 cacccgatga tcgagtggac acattcacct cttctcgatc tccctgtcca acgtccgaga
     1501 tatagaggtc tcctgtctgt cggtcaaaac caaagcgcca ggggtttcgg agcccaaggg
     1561 cccacatttc cgcgcggatt cctgcttggc cgatgcaggg atttgttgcg ggaatctcat
     1621 aagcggggag cggtgattgt gtcgcctgcg atacgtcgat tcggagcagt ttgcctaaga
     1681 ggtcgttgag attctgcgcg tggttttgag gatcgccccc gctaccccca tcgccgaagc
     1741 cgaggtaaag gaagccgtcc ggtccgaagg taatcatccc gccattatga ttggcaaacg
     1801 gttgcgccaa cgtcaggatg attcggtcct caggagtcag cgcccgctca gggttgcctg
     1861 gatcggccag gtatcgggca atgaccgacg tgccggtgac gtccgtgtaa ctcacgtaga
     1921 aacggcggtt gatggcatac tggggatcga aggccaaccc gagcagtccc tgttcactgc
     1981 cggctgaaac cagcgcgctg atatcgagaa acgtcgcttg gagagtgttc gtgttgcgat
     2041 cgaggatctt gatccggccg cctttctctg ccacgaacaa tcgagccatg tcacccggcg
     2101 gggccgtgag gaacagcggg aagctcaacg ttgtgctgat ctgctcaagg cgaagcagaa
     2161 ttgctggcgg cggagctccg ggtgacggcg ccggtggggg tacctgcgtc tgggccacaa
     2221 ggcaaggagc agaagctgcg acatttgatg ttggcggatt gtctccccct gaactgcaag
     2281 cagtgagcaa tgggaatagg aaatgcccga tgaacctcat gttgatcgaa tggctcaccg
     2341 cgttgtttcc ctcgccgaca ccaagatgtt gacccggcgt agaacttctt cgacccgccg
     2401 atttgctcag tttaccaaac acagaaagtc tcgtgtaatg gggtgattct gaagagagtg
     2461 gcgagtgtcc tgcaaattgg agcgactaag atgaagggcg taatttggtt tgatccgcga
     2521 agaaatcggt tcgtgttagc taccgctcct tcgtcacctg gtgcagttcg acggcgttgc
     2581 ctcctggatc aagaatcacg gccatgcggc acacaggcgt tgagaggggt tcaagcttga
     2641 cagtggttcc cttcgcacga ggtgttatca ccattgcctc cacgtcgtcc acttcaagcc
     2701 cgatctcgcc gccggcatcg gcgctgagat tcactccttc cgctgatgtg gtaatggcga
     2761 aggtactgcc accaatgtct tacccgatcc agacatcttg aatcactctc gcgacgttca
     2821 gaccgaagtc ttattcgtaa aactttctgc cgcgaccgat gtctgtgacc ggatacatgg
     2881 cgaatgcgat actctcgatc ataatcgtgc ttcggcttcc cgatcgcgcg aggtgtggag
     2941 ataggcgatc agctgctttt tatctcgctc ctgcatgttc accgcgatcc ttaactgttc
     3001 caggtattgg tccagcgttt tcccgcccag atcgatcttg cgggatgtga agaatgcacg
     3061 gacgtctcgt tcgagttccg gtgttgcgag ggctgcgatg cctccgcaca tacggcgcag
     3121 gccttgtttc ggaaaaagtc gatccatctg atcccaattg tttttgacga aggcccatgc
     3181 cagctcgcgt ccgtggacgt tgcccataac ggcgctgatc aggaacgggg catcctgggt
     3241 gcgagtttcc ccgttcagcg tgcgggccag cgtctgctcc agcaacggct tgtgtcgaaa
     3301 ctccgccagc gaaaacaggt agcggcgctc ttcttgcggt gtcgaggcgg tacggaaccg
     3361 ctcggtaaat tcctcgtaac gggcctggtc acccgtaaat gccagagtcg acacgacggc
     3421 ggggaccaga tcagggtgga tcgccgccgc gtttgttttg aacgcagcat agagttctgc
     3481 cgcgcgctgc tgggtcgcag gatcattccc aagtttcccg agtgcgcgga tcaactcgct
     3541 tcgtaattgt ccggtgagct cggattctcc tcgtttcggc gtccagccga cgtccatgac
     3601 ggcggggctc actcgttgcc gtaccagcga ttccaacgcc ggccgttcgt ccgtatcgat
     3661 gagacggttc aaaaacgcga aagagtctaa caggagagcc cacacgttct tgtcgcggtc
     3721 cgttgtgaac tgcgccgcga ggtcgagata ctcggtgatc ggcatgagtc cggcaacggt
     3781 aatggcccat gcatcgttga tcagcgcaaa ccgttccacg gcagccaatt tgtcgagccc
     3841 ggttttcaag agacgagcca agagatcggg cgcgtaacgg actcggtaga atccatgtcc
     3901 accttcgttg aggagcagag agtcgaactc gcgcggcact gggaatcgtg cttctgtctc
     3961 ttccaagagc agacgtgagg tgatggattt atctccagta acgactcgca tttgaaccgg
     4021 aatatgccac cgctgtcctc gcgcttgttg tgactgcgca tcatcgaggt aattgaaacg
     4081 ttgttggatc aataccagct cagacgactg attgacgctg gcggtgacaa gtggaaaacc
     4141 gggctggaaa atccatccgt ccatcaattc cggcactggt tgtttcgcca cctggcccaa
     4201 tgagatccag aggtctttcg tatcggcgtt gccgtaggcg tgcgttcgca agtagtcacg
     4261 cactccgtcc cggaacaccg tcgggccgat gtgctgctcc agcattcgca acaccgatgc
     4321 ccccttttcg tacgtcaaca cgtcgaacat cgcctccgcg tctttcggag cgcgaaccgg
     4381 atattcaatc ggtctcgtgt tctgaagccc gtcgaccgag aaggctgcgg cacgtgacat
     4441 gctgaacgac tcccagcgtt tccattccgg tttccaggcg tcgaccgcca acatttccat
     4501 gaaggtggcg aaggcttcat tgagccacaa tccgttccac caggacatgg tcacgaggtc
     4561 gccgaaccac atatgggcgt tctcatgggc gacgacgtcg gcgacccgct ccagctctgc
     4621 atgggtggcg gcgcgttgat ctacaaggag cgccgtctct cgaaacgtga tcgccccaag
     4681 attctccatc gcgcctgacg cgaaatccgg aatggcgaga aggtcgagtt tgccgccagg
     4741 ataggggatg tcatagtacc gttcgaagaa cgacagcgag gcggctgcaa tgtcctgtcc
     4801 aaaacgcgca aggtgggttt tcccaggaat tgcccaaagg cgaatcgggg tccgaccaac
     4861 catcgtcggg tcggtcgagt taagcgtccc caccacgaag gccacaagat acgtggacat
     4921 cttgatggtg tcagagaatc ggacgatctt cttcccgttt tcgacggttt gggacgcaac
     4981 cggggtattg gaaacagccg tcaacgcagg atcaacaatc aaggtggtgg caaacacagc
     5041 cttaaaatcc ggttcatccc aacagggaaa agctcggcgc gcatccgtgg cttcaaactg
     5101 tgtcgcggcg atcgagtggg tggtgcccgc tttatccttg tacgtgctcc ggtagaatcc
     5161 tcggagtttg tcgttcaagg tgcctgcgaa ggtcagtcga agggtccaat ttcccggagc
     5221 gagacgggtc gtgaaggcca gtctgcagcg ttgcagcgtg ccgtcgagtt caatcgtagc
     5281 ggtatgactg gttccatccg tggtccgaag cgaggcggtt cggatgtcga gatcagccgc
     5341 attgagcagg atctcggctg tcggctgagt gattgtcaac atgaccgttt cttctccgga
     5401 gaatgttgcg gtcttgagat ccggttcaag gagaaggtcg tatcggaggg gcacgacgtg
     5461 ccgcggaagc cgatacgggt cgaccgcctg cgatggagaa acatggttca tatcttcacc
     5521 tttcggccgt tcagcgataa gcgtggttgg tgataaagga agaacaaggc ca
//
LOCUS       DATDNW010000210         5895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_641652, whole genome shotgun sequence.
ACCESSION   DATDNW010000210 DATDNW010000000
VERSION     DATDNW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641652"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VI359_05245"
     CDS             complement(<1..378)
                     /locus_tag="VI359_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEY6973690.1"
                     /translation="MSLSETIKAEARSLGFDAVGICRLTDHPQYSAPSTQEDSLTQRF
                     YGRLQDWLQRGYHGTMAWMERDPAKRANPQRVLPGCRSIISVGMNYFTNHRAHDQPGF
                     GRIARYAWGKDYHQILGKKLVQLE"
     gene            complement(390..773)
                     /locus_tag="VI359_05250"
     CDS             complement(390..773)
                     /locus_tag="VI359_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973691.1"
                     /translation="MARIMVIQLLMPAFFLAILTVFGLTPAQVKAQPMDDPGATTELS
                     CSFGMAKGEAGQTCQVPFPQGCLVAHIPGTQKPWATVSKGGATRCKFDEKATDWKTRI
                     TGVCSRCTSPQCSAQFYVRFNCSGR"
     gene            860..1972
                     /locus_tag="VI359_05255"
     CDS             860..1972
                     /locus_tag="VI359_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEY6973692.1"
                     /translation="MLATRIRQKEGIFYFIAYKAVDLLEKVRFTSRYYFEGEEIVQGK
                     ISEHDEVAQFIAGIERSEKGFQRVLNRQKIKQIVNFYETVVAQPMIPGTILLFTDETL
                     RFQKAGDSESVGHLSEPKGKYLVIDGQHRLAGLHFFQAKHQDQIGNVEVPCVLFDGRS
                     GDFATEMFVIINSTHTRINRSHLVDLYEKVSWESPEKKFAAKVANLLYAEADSPLQYK
                     INRLGGRSKQEKWILQSEVFNELLKVVTAHKRWLESHLGMRADRCYALVRDYLKGVKD
                     VMEEVWGNNERYMFTRDVTLKALIRVLDDLIIDRKLMSDWDEQRSHKPFAELLKPWAS
                     LVKEFRAEGFYERFPAKGQLERVRRVHQRLLDAIVG"
     gene            1956..2561
                     /locus_tag="VI359_05260"
     CDS             1956..2561
                     /locus_tag="VI359_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6973693.1"
                     /translation="MRLWDRLLGLLTPLPVACLLWSFSIAAVETRPVFAGNVSETDSL
                     TVNVESGGGIRAIAQVFFPAKPEIIQAILSDYGHWPELFDVRMKVASLAIEHGVATTD
                     LRIEHALLPGERRLVSESRTLPGGGLMTDLKAGDFKRYHRVWKLGPGGDGSGTKADFE
                     LVVEIDSLVPDWLIAVAIRRELEAHFRIVKEKALARINEGK"
     gene            complement(2545..3723)
                     /gene="thiI"
                     /locus_tag="VI359_05265"
     CDS             complement(2545..3723)
                     /gene="thiI"
                     /locus_tag="VI359_05265"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEY6973694.1"
                     /translation="MRCVIVHYHELALKGRNREHFEQRLVRNIQWALKGLGVKRVESL
                     RSRIRVVVPPGVSEDIVKDRLVHVFGVANFLMARAVPLDLISPDLSELNNAIGHDLQH
                     HHFTTFRITAKRADKRLTLTSMDVERAVGKHLCATTGKKVSLTNPDLTVYIELLSKEV
                     YFSADKVAGPGGMPVGVSGKVACLISGGIDSPVAAYRMMKRGCRALFIHFSGRPLVSR
                     ASEEKVHDLVQTLTAHQYQSRLYVIPFGEIQREIVAQAPAPFRVVLYRRLMLRIAEDL
                     ARREQCWGLVTGDSLGQVASQTPENLSVIQEAAELPILRPLIGMDKVEITDQAQRIGT
                     FSISIEPDQDCCSLFTPAHPSTKTRLDDIRKIERVFDIGALVKQGLDKAELSEFTYPR
                     "
     gene            complement(3795..4718)
                     /gene="trxB"
                     /locus_tag="VI359_05270"
     CDS             complement(3795..4718)
                     /gene="trxB"
                     /locus_tag="VI359_05270"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY6973695.1"
                     /translation="MRQVVIIGSGPAGLTAAVYCARANLSPLLIEGWQSGGQLTTTTE
                     VENYPGFAKGIMGPELMKEMRAQAERFGTEFMTGDVTAVDLKTRPFTLTIDGEQTVQT
                     RTLIIATGASAFQIGLKNESRLTGHGVSTCATCDGFFFRGKELVVVGGGDSAMEEAIF
                     LTKFATKVSVVHRRDKLRASKIMQDRAMKNEKITFIWNSIIEDILGDDVVTAVRLKNL
                     VTGKTADLPCAGVFVAIGHRPNTTLFVGQLDMDEKGYIKTHTGTTTSVPGVFAAGDVQ
                     DSKYRQAVTAAGSGCVAAIDAERFLEGEGQH"
     gene            complement(4768..5388)
                     /gene="coaE"
                     /locus_tag="VI359_05275"
     CDS             complement(4768..5388)
                     /gene="coaE"
                     /locus_tag="VI359_05275"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY6973696.1"
                     /translation="MLCSAAMILVGLTGGIATGKTTVANMFKRCGAVVIDADALAREV
                     VQPGKPAWRAIVKTFGGNVLNPDQTINRHVLGHIVFRSRAKLRLLERIIHPRVARLQA
                     RLTRQAARKDPDAVIIYDVPLLFEVRINTRFDKIIVVTADRKKQVTRLKARNGLTRAV
                     ALQRIRSQMPLSKKVQKADYVLDGALSKSILRRNVRQLYKTLRTFA"
     gene            5455..>5895
                     /locus_tag="VI359_05280"
     CDS             5455..>5895
                     /locus_tag="VI359_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY6973697.1"
                     /translation="MKRMMVLCLMVVGGFWGTGGVYADDAAPLSPRTIVVALDGSGDF
                     TSIQEAVDAAKKGDVVFIKAGRYAQDVTIHSKEKVKLVGAGRDQVTLLGREEVVGALH
                     VGKWPYGATEIEISEMTINDHGGHAVGLFNGQGIILRHLTVNGML"
BASE COUNT         1260 a         1541 c         1774 g         1320 t
ORIGIN      
        1 ttcgagctgc accaattttt tgccgaggat ctggtgataa tccttgcccc aggcatagcg
       61 ggcaatgcgt ccgaaccccg gctggtcatg ggcccggtga ttcgtgaagt agttcatgcc
      121 gacagagata atggagcgac agccaggcaa cacgcgttgt ggattcgcgc gtttcgccgg
      181 atctcgctcc atccaggcca tcgtgccgtg gtagcctcgt tggagccaat cctggagccg
      241 accgtagaag cgttgagtca gggagtcctc ctgggtgcta ggtgctgagt actgaggatg
      301 atcggtgagg cgacagatac cgaccgcgtc gaacccgagt gatcgagcct cggccttgat
      361 ggtctctgac aaagacatgg gaagagcgac taccgaccgg agcagttaaa tcggacgtag
      421 aactgtgccg agcactgagg agaagtgcag cggctgcaga caccggtgat gcgagtcttc
      481 caatcagtgg ctttctcgtc gaacttgcag cgggtcgcgc cacctttgga aacggtcgcc
      541 cagggtttct gcgtgccggg gatgtgtgcg acgagacacc cttgggggaa cggtacctgg
      601 catgtttgcc ctgcttcgcc cttcgccatg ccgaaactgc aggacagttc cgtcgtcgca
      661 cctgggtcgt ccattggctg tgcttttacc tgggcagggg taagaccgaa tacggtcaga
      721 atggcgagaa agaacgcagg catgagaagc tggatgacca ttatgcgcgc catgctagca
      781 cagtctcgtg tcttgcggcc accggagggt ccgcgtagaa tctgttcgcc gagacgccac
      841 tcgaaaccac ggaggttcca tgcttgcgac tcgtattcgt cagaaagaag gcatcttcta
      901 cttcattgcc tacaaggcgg tcgatttgct ggagaaggtt cgtttcacca gccggtatta
      961 ttttgaaggc gaagaaatcg tccaaggaaa gatttccgaa catgacgaag tcgcgcagtt
     1021 catcgccggc atcgaacgaa gcgagaaagg ctttcaacga gttctcaatc gacagaagat
     1081 caaacaaatt gtgaattttt atgaaacggt cgttgcgcaa ccgatgattc ccggaacgat
     1141 cctgctgttt accgatgaga cgttacgttt tcagaaggct ggcgattccg agtccgtcgg
     1201 acatctgagc gaaccgaaag ggaagtattt ggtgattgat gggcaacacc ggttggcggg
     1261 gctgcacttc tttcaggcga aacaccagga tcaaatcggg aacgttgagg tgccctgtgt
     1321 cttgttcgac gggcgcagcg gcgattttgc gaccgaaatg ttcgtcatta tcaacagcac
     1381 gcacacgagg atcaatcgct cgcatctcgt cgatctctac gagaaggtgt cctgggagag
     1441 tccggaaaag aaattcgccg cgaaggtggc gaatctctta tacgcagaag ctgattcacc
     1501 attgcaatac aagatcaatc gactcggcgg ccgcagcaag caggaaaaat ggattctcca
     1561 atcggaagta tttaacgaat tactcaaagt cgtcacggcc cataagcggt ggctggagtc
     1621 gcacctgggg atgagggcgg accgctgcta tgcgctggtg cgcgactacc tgaagggtgt
     1681 gaaagacgtc atggaggagg tgtggggaaa caacgaacgg tacatgttta ctcgcgatgt
     1741 cacgttgaag gccttgatcc gtgtgttgga cgatctgatc atcgatcgca agctaatgag
     1801 cgactgggat gagcaacggt cacacaagcc gttcgctgag ctcctgaaac cctgggcgtc
     1861 actcgtcaaa gagtttcgcg ccgagggatt ttatgaacgg ttccccgcca agggtcagct
     1921 ggagcgtgtc cgacgagttc atcagcggtt gctcgatgcg attgtgggat agactcctcg
     1981 gtttgctcac gccattgccg gttgcctgtc tcctgtggtc gttctccatc gcggcggtgg
     2041 agacaaggcc ggtattcgcc ggcaacgttt ccgaaactga cagcctcacc gtgaatgtcg
     2101 agtccggagg aggtatccgg gcgatagcgc aggtgttctt ccccgccaag ccggagatca
     2161 ttcaggctat tctgagcgat tatggccatt ggccggagct ctttgatgtt cggatgaagg
     2221 ttgccagcct cgcgattgag cacggcgtcg ccacgacgga tcttcgaatc gaacatgcac
     2281 tgttgccggg agagcgtcga cttgtcagtg agtcacggac gctgcccggc ggcggactga
     2341 tgaccgatct gaaggccggc gactttaaac ggtaccatcg agtctggaag ttaggtccag
     2401 ggggtgacgg gagcgggacg aaagccgact tcgagctggt cgtggagatt gattcccttg
     2461 ttccagattg gctgatcgcg gtggcgattc gacgggagtt ggaggcacac ttcaggattg
     2521 tgaaagagaa ggctcttgcg cggattaacg agggtaagtg aattcggaca actcggcctt
     2581 gtcgagtcct tgcttgacga gcgcgccgat gtcgaacacc cgctcgatct tgcgaatgtc
     2641 gtcgagacgg gtttttgtac tggggtgtgc gggtgtgaag agtgaacagc agtcctgatc
     2701 cggctcgatt gaaatcgaaa atgtgccgat ccgttgggct tggtctgtga tctcgacttt
     2761 gtccatgccg atgaggggac gtaagatcgg cagttcagcc gcttcttgaa tcaccgagag
     2821 attttccggc gtctgcgagg cgacttggcc gagactgtcg ccggtcacca gtccccaaca
     2881 ttgttctcgt cgcgccagat cctcggcgat ccggagcatc aggcgccggt acagcaccac
     2941 gcggaatggc gctggggcct gggccacaat ctcgcgttga atttccccga aggggatgac
     3001 atagagccgg gattggtact gatgggctgt caaagtctgt accagatcgt ggaccttttc
     3061 ttctgaagct cggctgacca acggtcggcc cgaaaaatgt atgaacaatg cacggcaacc
     3121 gcgtttcatc attcgatagg cggcaaccgg cgaatcgatg cctcccgaaa tcaaacaggc
     3181 cacctttccg ctgacgccca ccggcatccc gccgggcccg gccactttat cggcggagaa
     3241 gtagacctcc ttcgaaagca gttcgatgta tacggtgaga tccggattcg tcaaactcac
     3301 ttttttgccc gtggtggcac atagatgttt gccgactgct cgctcaacat ccatcgaggt
     3361 gagggtaagc cgtttatccg cgcgtttcgc cgtgatgcgg aaagtagtga agtggtgatg
     3421 ttgaagatca tggccgattg cattattcaa ttcactgagg tctggagaaa tcagatcaag
     3481 cggaaccgca cgggccatca agaaattggc aaccccgaat acatggacga gccgatcttt
     3541 gacgatatct tccgaaacgc ccggtggaac gacgacgcgg atacggctcc gcaggctttc
     3601 aacgcgtttc acgccgaggc ccttgagagc ccattggata ttgcgcacga gacgctgttc
     3661 gaaatgttcg cggttgcggc ctttgagagc gagctcgtgg taatggacga tcacgcaacg
     3721 cattcgtagc tctcagccat tagctctcag caccagctcg cccttctggc tgagtgctga
     3781 aggctgacag tgcttcaatg ttggccttct ccttccagaa accgttctgc gtcgatggct
     3841 gccacgcagc cggaaccggc ggcggttaca gcctggcgat atttcgagtc ctgcacatcg
     3901 ccggcggcaa acactccggg aacgctggtc gttgttcctg tatgtgtctt gatatagccc
     3961 ttttcatcca tgtcgagctg cccaacgaac aatgtcgtgt tcggtcgatg gccgattgcg
     4021 acgaacacgc cggcgcacgg taaatcggca gtcttgcccg tgacgagatt cttgaggcgg
     4081 acagccgtga cgacatcgtc cccgagaata tcctcgatga tcgagttcca gataaacgtg
     4141 attttttcgt tcttcatggc gcggtcttgc atgatcttgg acgcgcgaag cttgtcccgg
     4201 cggtggacga cagagacttt ggtggcgaat ttggtgagga agatcgcttc ctccatcgcg
     4261 ctgtcgccgc caccaacgac gacgagttcc ttgcctcgga aaaagaatcc atcacatgtc
     4321 gcacacgtgg agacgccgtg cccggttagc cgcgattcat tcttgagccc aatctgaaag
     4381 gctgacgcgc cggtcgcgat gatcagcgtc ctggtttgaa ctgtctgctc cccgtcgatg
     4441 gtcaaggtga agggacgtgt ttttagatca acggcggtga cgtcgccggt cataaactcg
     4501 gttccgaatc gttcggcttg agcccgcatc tctttcatga gttcaggccc catgattccc
     4561 tttgcgaagc caggatagtt ctcaacttcg gtggtcgtcg tcagttggcc acccgattgc
     4621 cagccctcga tcagcaatgg cgacaagttc gcccgcgcgc aatagaccgc tgcggtgaga
     4681 cctgctggcc cggagccgat gatgacgact tggcgcatgc ctgcaatcta gcacagggca
     4741 ggagcggaat gcatagggaa cggtcgatta ggcgaaggtc cgaagggttt tatagagctg
     4801 tctgacattc cggcgcagaa tactcttaga gagggcgccg tccagcacat agtcagcttt
     4861 ctggaccttt tttgaaagag gcatctgact tcgaattcgt tgaagtgcca cggcacgggt
     4921 caagccgttt cgtgccttaa ggcgggtgac ctgcttcttg cgatccgcag tgacgacgat
     4981 gatcttatcg aatcgtgtgt tgattctaac ttcgaaaaga agcggaacat catagataat
     5041 gacggcgtcg ggatcttttc tggcagcctg tctcgtcagg cgcgcttgca gccgggcgac
     5101 gcggggatgg atgatgcgtt ccagcagccg aagcttggct cgactcctga acacgatgtg
     5161 gccgagtaca tgacgattga tcgtctgatc cggattcagc acgttcccgc cgaatgtctt
     5221 gacaatcgca cgccacgccg gctttccagg ttggacgacc tctcgtgcga gtgcatcggc
     5281 gtcgatcacg accgcgccgc atcgcttgaa catgttcgcg accgtggtct tgccggtggc
     5341 aatcccaccc gtcagtccaa cgagaatcat ggcggcggag catagcacgc gactttctct
     5401 gtcacaacaa accccgattg acttccggag gggcgcgcct gtatgagttt ttgcatgaaa
     5461 cggatgatgg ttctatgtct catggtggtg ggtgggttct gggggacggg aggcgtgtac
     5521 gctgatgacg cagccccgct cagtccccgg actatcgtcg tggcgctcga tgggagcgga
     5581 gatttcacgt cgatccagga agctgtcgat gcagccaaga agggcgacgt cgtcttcatc
     5641 aaggctggcc ggtatgctca agatgtgacg attcatagta aggaaaaagt caaacttgtg
     5701 ggtgctggac gggaccaggt cacgctcctc gggcgtgaag aggttgtcgg ggcccttcat
     5761 gttggtaaat ggccctatgg cgcgacggaa atcgaaatca gcgaaatgac gatcaatgat
     5821 catggcggac atgccgttgg tctattcaat gggcagggga ttattcttcg gcatctcacc
     5881 gttaacggca tgctc
//
LOCUS       DATDNW010000211         5104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_642229, whole genome shotgun sequence.
ACCESSION   DATDNW010000211 DATDNW010000000
VERSION     DATDNW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642229"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..520)
                     /locus_tag="VI359_05285"
     CDS             complement(122..520)
                     /locus_tag="VI359_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6973698.1"
                     /translation="MKVKKLLHTRMRVSDMDQTIHFYTTVLGLEVIEQKVSPRGSHLA
                     FLKVPNSDELIELCSFPASGPVKVQEDLVHLAFQVDNLDETIAQLTEKQIKITDGPTK
                     TSSGSRFIFIDAPDGYEVELIERPPGVKIV"
     gene            complement(759..1232)
                     /locus_tag="VI359_05290"
     CDS             complement(759..1232)
                     /locus_tag="VI359_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6973699.1"
                     /translation="MITPTIRAMTPEDRPAVIALLQDSDPWKRLGYRAGDWDLYFATI
                     PQGRDSYVADLDGRVAGIAIVRQKFLLGDYLELFGVADWARGKGLGKRLLEQIESIVF
                     ARANNLFACVSDFNTQARAFYQKLGYQEIGPLPNLLIPGSAEILLRKTAGPARKS"
     gene            complement(1229..1936)
                     /locus_tag="VI359_05295"
     CDS             complement(1229..1936)
                     /locus_tag="VI359_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY6973700.1"
                     /translation="MLLARSRRVCLIAVAFVLILIVPAQGQVIKSGPPACPGVALTFD
                     LCPVRGGTGYDQALIDYLIEHKIPATFFLSGKWIARHDRQVRALLRIPFFEVGTHGEV
                     HAHLPMHPVDEQKQEILGPVRLLKTKYGHHATLFRPPYGEFNDETVEVVHALGLQFIL
                     WNIVSGDPDPTLSAEQIDDRLTRMLRKGGVIVMHANGRGIHTREVVEHLHQTLLPQRG
                     LQPMTVTDLMNCSQTMP"
     gene            complement(2015..3370)
                     /locus_tag="VI359_05300"
     CDS             complement(2015..3370)
                     /locus_tag="VI359_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973701.1"
                     /translation="MEDFGKDMLMLGGTLAGFVAALLTVLEKMIDIQKRLKPNKERRS
                     PAPSERPSIESSSTVDFFSSKPLRGSSYLLLNETSVIIAAGLLLNYVGLTLSRHLESI
                     LFLDMTGTALVAFLMGPWWGAITALLSNSVVNWLLYPETGADVVIFPWSIVSMAGAFF
                     WGWMGRTAGFQKYLKTGRSSVWSHAWFLYMFGVGGALVMSLPGTFVQAALQERSTFAL
                     NPDVAETLSFRVLQWEQAIRGYLEAQFGLMHGESLSWWVVNWFQTWVRYIPDKTMSAA
                     IALVVLKYGYPLFERELIHGGPEGERPKDERILPLVLGLTFAPSFAVLISNETYGGLT
                     WWPLWSVPWLLMLLGYVSLRYWGPTDATLHEARLQRAERYARALKPIGKEASYEFCRR
                     LTFVTLIASLLFALCLPILLMDFYRVTFKFFCVVYGFLLVVHLIRVAIAQNISIARAD
                     G"
     gene            complement(3481..4350)
                     /locus_tag="VI359_05305"
     CDS             complement(3481..4350)
                     /locus_tag="VI359_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY6973702.1"
                     /translation="MQPLRFLIDLGKSFLHNGCASLAASLAFFSLLSLFPLVFLLLYG
                     LSFVVSQEVISEQVLLSFLKGFLPSLGEHVAEELHRVGSLETVRWAVFLAFAWFGALV
                     FYELDYALNVVFESTWRRHPLISTMIAVASLGATGLLLIVSYVATQTVNFMIAYAPRL
                     WGLNLIALAAHQFFLTYTLPFALAFLSVTVLYRFVPRRRPQWREAMIGALTFSLLWVS
                     AKLLFVTYGTYATIYTHLYGSLFEVILLLLWVYYSSALLLIGAGVAHHLQQRARALSA
                     AAEKSPQPDPALP"
     gene            complement(4409..>5104)
                     /locus_tag="VI359_05310"
     CDS             complement(4409..>5104)
                     /locus_tag="VI359_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973703.1"
                     /translation="EGLGGLKANWSMLFMLLCVVTLVLGNVVAIIQTNIKRMLAYSSI
                     AHAGYALLGVVAAGRGVGESGSATGLASVMLYLTLYAFMTLGAFTVISMLRKGGLEGE
                     EIDDFTGLAKRQPLAAFLMLVFMVSLAGIPPTAGFIGKFYVFMAAVEAGMTWLAVVAL
                     VFAAVSAYYYMRVVMVMYMREPDVSTLESPQLVTSPALSIVLVCAVAGVIVFGVFPNP
                     LVSLALQSVLSLK"
BASE COUNT         1215 a         1422 c         1490 g          977 t
ORIGIN      
        1 ggccatgccc gcggaaaaat ccgtttttca tgtgtcgccc cttcccgtga ccgatgctca
       61 cacaggcggt ggaaggtatc atgaagagga ggagcgggaa aagggccggg aaggaggttc
      121 gtcagacaat cttcaccccc ggcggtcgtt cgatgagctc gacttcgtag ccgtccgggg
      181 catcaataaa aatgaagcgg ctgccggagg atgttttcgt tgggccatcg gtaatcttga
      241 tctgtttttc ggtaagctgt gcgatggtct cgtcgagatt gtcgacctgg aacgccaggt
      301 gaacgaggtc ctcttgcacc ttcactgggc cgctggctgg aaaactgcac agttcgatca
      361 gttcgtcgct gttgggtact ttgaggaatg ccaggtgcga tccccgcggc gataccttct
      421 gctctatgac ttcgagcccg agcacggtgg tgtagaagtg gatcgtctga tccatgtcac
      481 tgacacgcat tctcgtgtgg aggagcttct tgaccttcat gatgtccgtt agtaatagtc
      541 agctttcagc aaacagctca gccctctctt tacccctcct tgataaggag gggctagggg
      601 aggttcagta tgaccttcgc tgtctaattc gttcacgacg gaccgttgga gatggttggc
      661 ttgtcgtcgt gtgactctgg gtatgttgcg ctacccctcc tagcctccct ttacaaaggg
      721 gaggcataat ttaagtagct cattgctgac agctatgttc agctcttcct cgccggcccc
      781 gccgtctttc gcagcagaat ctcggcactc cccgggatga gaaggttcgg tagaggaccg
      841 atttcctgat aaccaagctt ctgatagaag gctcgtgcct gtgtgttaaa gtccgacacg
      901 caggcaaaca ggttgttcgc tcttgcgaag acgatggact cgatctgttc gagcaagcgt
      961 ttccctagac ccttgcctct cgcccaatct gccactccga agagttccaa gtagtcgccc
     1021 aacaggaatt tttgccggac gatcgcgatg cccgctaccc tcccatcgag gtcagccacg
     1081 taactgtccc gtccctgagg gatcgtagca aagtacagat cccagtcgcc agcccggtaa
     1141 ccaagtcgtt tccatggatc agagtcttgc agcagtgcga tcacggcagg acggtcttcc
     1201 ggcgtcatcg cacgaatcgt cggggtgatc atggcattgt ctggctacaa ttcatgagat
     1261 cggtgacggt catgggctgc agccctcgct gcggcaagag agtctggtgg aggtgttcga
     1321 cgacttcacg cgtgtgtatg cctctcccat tggcatgcat gacgatcaca ccacccttac
     1381 gaagcatccg cgtgagtcga tcatcgatct gttcagccga aagggtggga tccggatcac
     1441 cggatacgat attccacaga ataaattgca gaccgagagc atggacgacc tcgacagtct
     1501 catcgttgaa ttcgccgtag ggggggcgga acagcgtcgc gtggtggccg tacttggtct
     1561 tgagcagccg gaccggtcct aaaatttcct gtttttgctc atcgaccgga tgcatcggca
     1621 agtgagcgtg aacctcgccg tgggtgccga cttcaaagaa gggaatccgc agcagcgccc
     1681 gtacttgacg atcgtgtcgg gcaatccatt taccggacag gaagaaggtg gcggggattt
     1741 tgtgctcgat caaataatcg atgagcgcct gatcgtagcc tgtcccgccg cgaaccgggc
     1801 aaagatcaaa ggtgagcgcc acgccggggc aggccggcgg ccccgacttg atcacctgac
     1861 cctgcgccgg cacgataagg atcagcacaa aagccacggc gatgaggcac acccgacgtg
     1921 aacgagccag cagcatggtt cctagtatac ccgaaccagg caggcgccgc cgagtcgcgc
     1981 tcgctatccg gcaaaagagt cggtgagttg ggaattatcc gtcggctcga gcgatggata
     2041 tattctgggc gatggcgaca cgtatgagat ggacgacgag caggaaacca tacacgacgc
     2101 agaagaactt gaaggtgacc cgatagaaat ccatgaggag aatgggcagg cagagcgcaa
     2161 acaggagact ggcgatcaac gtgacaaacg tcagccgacg gcagaattcg tacgacgctt
     2221 ccttgccgat tggtttgagt gctcgcgcgt accgttcggc ccgctggagc cgtgcttcat
     2281 gcagcgtcgc gtccgtcggt ccccaatacc gcaaactgac atagcccagc aacatcaaga
     2341 gccatggaac tgaccagagc ggccaccacg taaggcctcc gtacgtctcg ttgctgatca
     2401 gtaccgcaaa cgacggtgcg aacgtcaatc ctaacaccag cggaaggatc cgttcgtcct
     2461 tggggcgctc gccttccggt cctccatgaa tcaattcccg ctcgaagagg gggtagccgt
     2521 atttcaggac gacgagcgca atcgcggcgc tcatggtctt gtcgggaatg taccgaaccc
     2581 acgtctgaaa ccagttcacc acccaccagc tcaggctctc cccgtgcatc aggccgaact
     2641 gtgcctccag gtaccctcgg atggcctgtt cccactggag aacacggaag ctcagggttt
     2701 cagccacgtc tgggttcaaa gcaaacgtcg atcgttcttg caacgccgcc tgcacgaacg
     2761 ttccgggcaa actcattacc agggccccac cgactccgaa catatagaga aaccaggcat
     2821 gcgaccagac ggagctgcgg ccggtcttga gatacttttg aaaccccgct gtacgcccca
     2881 tccatcccca aaaaaaagcc ccagccatac tgacaatcga ccaggggaaa atcacaacgt
     2941 cagcgccggt ttccgggtag agcagccagt tcacaactga attggaaagc agtgccgtga
     3001 tcgctcccca ccaggggccc atgaggaagg ccaccaacgc cgttccagtc atgtccagga
     3061 agagaatgct ctccagatga cggctcagcg tgagtccgac ataattcagc aagagtccgg
     3121 cagcgataat cacgctggtt tcattcagga gcaaatagga tgatccccga agcggcttcg
     3181 aggaaaagaa gtcgacggtc gaagaggatt cgatggaagg gcgttcagac ggggctggtg
     3241 acctccgctc tttgttcggt ttgagccgtt tctggatgtc gatcatcttt tccagcacgg
     3301 taagcagggc tgccacgaac cctgccaggg tcccgccgag catcagcata tccttgccga
     3361 aatcttccat gatgaatagc tcccttcgtg ccggatgcca ttctagccag gaagggagtt
     3421 ccctgccaga aaaaggccgg aagtcgacag gcctggaaaa cccgaagatg gcgggtggtg
     3481 ctacggcaaa gcgggatctg gctgaggaga tttttcagcc gcagccgaaa gtgctcgggc
     3541 gcgttgttgc aggtgatggg ccaccccagc tcctatcagc aaaagggctg aagagtaata
     3601 gacccataag agcaagagaa tcacttcaaa gagcgaccca tagagatggg tgtagatcgt
     3661 tgcataggtg ccataggtca cgaatagcaa tttggcggag acccacagca ggctgaacgt
     3721 gagtgcgccg atcatggctt cacgccattg tggtcgacgg cgaggaacga accgatacaa
     3781 caccgtgacc gacaagaagg cgagggcaaa gggcagggta taggtgagaa aaaactggtg
     3841 ggccgccagc gcgatcaagt tcagccccca cagtcgagga gcataggcga tcatgaagtt
     3901 caccgtttga gttgccacgt aggacacgat cagcaacagg cccgtggcgc ccagcgaggc
     3961 cacggcaatc atagtggaaa tgagagggtg ccgacgccac gtgctttcga agaccacgtt
     4021 caatgcgtag tcgagttcat aaaataccag ggcgccgaac caggcaaacg caaggaagac
     4081 ggcccaacgg acagtttcta aggaaccgac tcgatgtagt tcttccgcca catgctctcc
     4141 cagggacgga aggaacccct tgagaaagct gagcaagacc tgctcactga tgacttcctg
     4201 gctcaccaca aaactcaacc cgtacagcaa cagaaagacg agtgggaaga gcgaaagcag
     4261 ggaaaaaaag gccagcgagg ccgccaggct tgcgcagcca ttgtgaagga acgatttccc
     4321 gaggtcgatg agaaaccgca agggttgcac gggcacagcg tagcacgagt cgagcgttcg
     4381 tgcacggatt cgggaaggtc gagaggcctt acttcagtga gagcaccgat tggagagcga
     4441 ggctgactaa gggattggga aagaccccga acacgatcac gccggctacg gcgcagacta
     4501 gcacgattga cagagcgggt gaggtcacga gctgtggaga ttcgagagtc gacacatccg
     4561 gttcgcgcat atacatcacc atgaccacgc gcatgtagta gtaggctgac acggcggcaa
     4621 agaccaaggc gacgaccgcg agccaggtca taccggcttc gactgcggcc atgaagacgt
     4681 agaacttgcc gatgaaaccg gccgtcggag gaatgccggc caacgaaacc atgaagacca
     4741 gcatgagaaa tgccgccagc ggctggcgct tggcaagtcc tgtgaaatcg tcgatctctt
     4801 ccccttcgag tccgcccttg cggagcatgc tgatcacggt gaatgcgccc agggtcatga
     4861 atgcgtagag ggtgaggtat aacatgacgc tggcgagacc ggtcgcgctt cccgattcgc
     4921 cgacccctcg gccggccgca acaactccga gcagcgcata gcccgcatgc gcgatgctgg
     4981 aataggccaa catgcgcttg atgttggttt ggataatggc gacgacgttg cccagaacga
     5041 gggtgaccac gcacagcagc atgaacagca tcgaccagtt ggctttcagt ccgcccagcc
     5101 cctc
//
LOCUS       DATDNW010000212         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1119652, whole genome shotgun sequence.
ACCESSION   DATDNW010000212 DATDNW010000000
VERSION     DATDNW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119652"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..805)
                     /locus_tag="VI359_05315"
     CDS             complement(95..805)
                     /locus_tag="VI359_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973704.1"
                     /translation="MSFDAELLTILGLGFVLGLRHALDTDHIAAVSIVLARRPSVRAS
                     GIIGFSWGLGHTLVLLLVGAVVLALRIEIPEPLARAAEFGVGVMLVVLGSALAFKLSK
                     EQWHFHPHEHDGERHFHLHSHHLNADHRHSHWLRDSIQPLCIGMVHGLAGSAALLLVV
                     LSTAKNMTAGLLYIIVFGAGSILGMMLIGLAISVPVLWSLSFGRQVFLAVQGLASLGS
                     IGIGIALMVQIVLRNQLL"
     gene            complement(862..1929)
                     /gene="moaA"
                     /locus_tag="VI359_05320"
     CDS             complement(862..1929)
                     /gene="moaA"
                     /locus_tag="VI359_05320"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY6973705.1"
                     /translation="MNTQQTLSSSLPPVADTFGRPLKSLRLSVTDRCNLRCKYCMPEE
                     EYVWLPREDVLTFEEMSTLTGYFTDVGVDRIRLTGGEPLLRRDLPRLVRRLMQNRKIM
                     DIALTTNGILLADQAQDLYDAGLHRITVSLDTLRPERFRQLTRRDEYARVLEGIESVG
                     RVGFTGLKLDTVAIRGFNDDELVQLIEFAKHYRAEIRFIEYMDVGGANDWSLNKVLSR
                     AAMLQILGKHYGHIEPLPERGSAPAQRFQLRDGTVFGIIPSTTTPFCSTCDRSRVTAD
                     GMWYLCLYATVGTDLRKPLRAGASPEEMWAIIRTGWEARRDRGAEARKALEPVGLRDG
                     GLIGIDRLREDPHLEMHARGG"
     gene            complement(2109..>2679)
                     /locus_tag="VI359_05325"
     CDS             complement(2109..>2679)
                     /locus_tag="VI359_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY6973706.1"
                     /translation="IGELILKKKILVSVNQEIAHEDMTIHDGDEVALLPPFAGGCLMD
                     QVSDDAQFVRVQREDFSVDEEIGRVRARSKRIGGISIFLGTARDRSKGREVDSITFEH
                     YEGMAQKKLREIRERALKDFDVIEVLVLHRYGEIGIGENIVLIVVGAEHRAEAFRACK
                     WAIDELKQITPIWKLEHTPEGEVWVEEHP"
BASE COUNT          619 a          843 c          691 g          526 t
ORIGIN      
        1 ctgatcccct ctcggcttga ctatgctgcg gagaatacca taccgccctc gatgacgcac
       61 gaggatttcc gagatcagac catgcctcga ttcgtcacag cagttggttg cgcaaaacaa
      121 tctgcaccat gagggcgatt ccgatcccga tactgccgag actcgccagc ccttgcacgg
      181 caagaaagac ctgtcgtccg aaagacagag accacaagac cggaaccgag attgcgaggc
      241 cgatgagcat cataccgaga atggagccag ctccgaagac gatgatatag agcaacccgg
      301 ccgtcatgtt cttcgccgta gaaagcacca cgagcagcaa cgccgccgat cccgcaagac
      361 cgtggaccat accaatacag agcggttgga tcgaatctcg caaccaatgc gaatgacggt
      421 gatctgcgtt gagatggtga ctgtgcaaat ggaaatgtcg ctcgccatca tgctcatgcg
      481 ggtggaaatg ccattgctct ttggaaagtt tgaacgcaag cgctgagcct agaacaacca
      541 acatcacccc tactccgaac tcggctgcgc gtgcaagagg ctcaggaatc tcgatccgta
      601 aggccagcac gacggccccg accagcagta ggacaagtgt atgcccgagt ccccagctga
      661 acccaattat gccggacgcc cgcacggagg gccgccgtgc gagaacaatc gaaactgcag
      721 caatatggtc cgtatcaagc gcgtggcgaa gccccagcac aaagcccaat ccgagaatgg
      781 tcaatagctc tgcatcaaaa gacatgaaag aacacgatcc ggggtggagc ggtctgaatc
      841 catgcaagga cctcgcgcgc gtcacccccc gcgcgcgtgc atttcaaggt gcggatcttc
      901 acggagccga tcgataccga tcaagccgcc atcgcgcagc ccgactggtt ccagggcttt
      961 gcgtgcttcg gcaccccgat cgcgtcgagc ttcccatccc gtcctgataa tcgcccacat
     1021 ctcctcaggc gaagcgccgg cccgcaacgg ttttcggaga tcggtgccaa ccgtagcata
     1081 gaggcagaga taccacattc cgtccgccgt cacgcggctt cggtcacacg tcgagcagaa
     1141 cggggttgtt gttgacggaa tgatgccgaa tacggttccg tcgcggagtt gaaaccgctg
     1201 agccggagcc gagccccgct ccggcagagg ctcgatgtga ccgtaatgct tgccgagaat
     1261 ctgcagcatc gcggcgcgcg ataagacttt atttagcgac cagtcgttgg ccccgccgac
     1321 atccatatat tcgataaacc gtatttctgc ccgataatgc ttggcaaact cgatgagttg
     1381 aacgagttcg tcgtcattaa acccgcgaat ggcaacggta tcgagcttca atcccgtaaa
     1441 cccaacgcga ccgaccgact cgatgccttc caacacacgc gcatattcat cgcgacgcgt
     1501 cagctgacga aatcgttcgg ggcgcaacgt gtccaaactc acggtgatgc gatgcaaccc
     1561 tgcgtcatac aaatcctgcg cttgatcggc aaggaggatc ccatttgtgg tcaacgcgat
     1621 gtccataatc ttgcggtttt gcatcaaacg ccgaaccaat cgcgggagat cgcgccggag
     1681 cagcggctct ccccccgtca gacgaatccg atccacaccc acatccgtaa aatagcccgt
     1741 cagcgtcgac atctcctcaa acgtgagcac gtcttcccgc ggcagccaga catactcctc
     1801 ttccggcata cagtacttgc agcggagatt gcagcggtcc gtcacggaaa ggcgtaagct
     1861 cttgagcggc cgcccgaacg tatcggcaac cggagggagc gatgaactca gtgtctgttg
     1921 cgtattcata cgttgactag aggatggtca aacaggctac ccttctcacc cgcccaaccc
     1981 ttggtgcgcc gggacgcgcc ttgtcccaga caaggccgca gcaagcaagg atctgaggcg
     2041 tactcttctg ccgtacgtcg aggatccgag cgctgcgaga acgatgctgg agaccttttt
     2101 caccatcctc atggatgttc ctcgacccag acctcccctt ccggcgtgtg ttccaacttc
     2161 cagattggag tgatctgctt caactcgtcg atcgcccatt tgcatgcccg gaacgcttca
     2221 gcccgatgct cggcgccgac cacgatgagc acaatattct caccgatccc gatttccccg
     2281 taccggtgga ggaccagcac ttcaatcacg tcgaaatcct tgagtgcccg ttcacgaatt
     2341 tctcgaagct ttttctgcgc cataccttcg taatgttcga aggtaatact gtccacctct
     2401 cggcccttcg atcgatcgcg tgccgtaccg agaaagatcg agatcccgcc gatccgcttc
     2461 gaccgagctc ggacgcggcc gatctcctcg tctacggaaa aatcctcccg ttgcacccgg
     2521 acgaactgag catcgtcact gacctgatcc atgagacatc ctcccgcaaa cggcggcagc
     2581 agcgccactt catcgccatc gtgaatcgtc atgtcctcgt gtgcgatttc ctggttcacc
     2641 gacacgagga tttttttctt gaggatcaat tccccgatc
//
LOCUS       DATDNW010000213         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_95665,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000213 DATDNW010000000
VERSION     DATDNW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95665"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VI359_05330"
     CDS             <1..495
                     /locus_tag="VI359_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEY6973707.1"
                     /translation="KPWPSMMRTIYKDPERYKTYWNTIPNCYTAGDVCHKDADGYMWF
                     MGRADDVIKVAGNRLGTAEVESALVSHPAVAEAAVIGKPHKTVGESIKAFIILKHGEL
                     ASSELIQSIKDQVMKELGKIAVPAEIDIVASLPKTRSGKIMRRVLKAKELGQDPGDIS
                     TIEE"
     gene            542..1012
                     /locus_tag="VI359_05335"
     CDS             542..1012
                     /locus_tag="VI359_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY6973708.1"
                     /translation="MVMRCSWKMGLVAGMVAVGFLCGTLGMAQAAESDTKAKKPKASQ
                     EPKAAKPKAEKPKFKTAETAGKAPACFGVAPKIEKVSPDEGKAGDKVTITGANFGSAA
                     CLHSVSFGPGHTAMYKMESATKITATVPSGGRKGLAILTVTTASGEDSKPFLMK"
     gene            complement(1078..1731)
                     /locus_tag="VI359_05340"
     CDS             complement(1078..1731)
                     /locus_tag="VI359_05340"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY6973709.1"
                     /translation="MFTGIVEEMGAVTAVEKTLAGTRLTILAATVLGDLKIGDSLSVN
                     GTCLTVVTKNERNFVVEISPETLSVTTLGQLNAGAPVNLERAMKLNERIGGHLVAGHV
                     DGVGTIRSRHQEGNAIFFTIEAPDEILRYCVVKGSVTVDGISLTINDVTDKGFSVAII
                     PHTAKVTTLGLKQIGDTVNLESDLIGKYVERLLQERNQLPKSIPVIDKDYLQKHRLI"
     gene            complement(1893..3062)
                     /locus_tag="VI359_05345"
     CDS             complement(1893..3062)
                     /locus_tag="VI359_05345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6973710.1"
                     /translation="MTPARSYVLLCTIGVFCFISYNLVRMPVLALFAESLGAGPERIG
                     LIVSVSTLTGVFLKLPSGALSDIYGRRLLLRIGVVAFGLPPFLYPFVSDLNVLTTLRF
                     FHGLATAIFAPSALATVAELYRERRGAALGTYTACTQSGALLGPFIGGYLVYAAGFSS
                     AFVTAGIFGCIAVVIFYSLRLTPPPPRLHERGFAPLLAEMWKGFTAVAKNRKVLITSS
                     TDAAKMIANGALMAFLPLYGVAAGLNPGEVGLLFTVQAFTSFFSKPIMGRVSDRVGRQ
                     PLIMIGLLICAATFVCIPHVSLFPFLLVLSAGFGFGEAVVSSSSSALVADSSEFKTLG
                     AGMGMQGTIMDIGHASGPLLAGVLIERMSYANAFAIIAGVQLLAAGVFWLTMRRA"
     gene            complement(3059..>3988)
                     /locus_tag="VI359_05350"
     CDS             complement(3059..>3988)
                     /locus_tag="VI359_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY6973711.1"
                     /translation="GAGFSGDAYLERWQARLGEDYVLACPTYPAGAWFTRRAEDLVLA
                     TIHLVQRRYHIDPDRIFLTGMSNGGIGTWLIGMHQAPTFAGIAPMAGGLDDALLPFLA
                     NLQSTPVYMIHGAKDQVMPVDLSRTISRELTGLGYPHVYREHQREHPMAGGHYFPREE
                     LPALVAWFNAQRRNPFPATIRLVREASHFQAFGWVRIDATDQIAAFSEDLINKRDELI
                     KRKQYAKLEASIKAPNRIEVETMRVQRYSLFLNEHLIDSAKPVTVLTNGQVTFEGLVA
                     PKVEILLRQARQRQDQRQLFPIQLNLVVPSQPS"
BASE COUNT          980 a         1149 c         1116 g          743 t
ORIGIN      
        1 aaaccctggc cctcaatgat gcggacaatt tataaagacc cggagcgcta taagacctat
       61 tggaatacaa ttccgaactg ctatacagct ggcgacgtgt gccacaagga cgcggacggc
      121 tatatgtggt tcatgggccg cgcggacgac gtcattaaag tggcgggcaa ccggctcggc
      181 acggccgaag tggaaagcgc acttgtcagt caccctgcag tggcggaagc agcggtcatc
      241 ggcaagccac acaagaccgt cggtgaatct attaaggcct tcatcatttt gaagcatggc
      301 gaactggcca gctctgaact gattcagtcg atcaaggatc aggtcatgaa agaactgggg
      361 aaaatcgctg tcccggctga aatcgatatt gtggcgtcgc tccccaagac tcgctccggc
      421 aaaattatgc ggcgagtgct gaaagccaag gaactcggtc aagaccccgg cgatatttcg
      481 accatcgaag aatagccgtc agtcgtcagc ttgcagcaat cagccaacca aggaggcggg
      541 tatggtgatg cgatgtagct ggaagatggg gttggtggcg ggcatggtcg ccgtcggatt
      601 cctgtgcggc actctgggca tggcacaggc agcggagtcg gatacaaagg ccaagaagcc
      661 gaaagccagt caggaaccaa aagcggcaaa accgaaggcc gaaaagccca aattcaagac
      721 ggctgaaacg gcgggaaaag ccccagcctg tttcggcgtt gccccgaaga tcgaaaaggt
      781 cagtccggat gagggcaaag ccggcgacaa ggtgaccatc accggagcaa atttcggaag
      841 cgctgcctgc ctgcatagtg tatcgttcgg tcccggtcac acagccatgt ataagatgga
      901 gagcgcgacg aagattaccg cgactgtccc gagtggcggt cggaaggggc tggccattct
      961 aacggtgacg acggcgtcag gcgaagattc caaaccattt ctcatgaagt aacgatcgca
     1021 gagggtcgag taatcggctc tcaccagaac agcacgtcga gagccaatag cgatccatca
     1081 aatcaaccgg tgtttctgca aataatcttt gtcgatgaca gggatggact tagggagctg
     1141 atttcgttcc tgcagtagtc gctcgacata tttgccgatg agatcggatt cgagattgac
     1201 cgtatcgccg atctgcttga ggccgagcgt ggtgaccttg gccgtgtgcg ggataatagc
     1261 gacggagaac cccttgtcag tcacgtcgtt gatcgtgaga ctgatcccat cgaccgtgac
     1321 tgatcccttg acaacacagt agcgcagaat ctcatccggc gcctcaatcg tgaagaagat
     1381 ggcgttgccc tcctgatgac gactgcgaat ggtgcccacc ccgtcgacat gaccggcgac
     1441 gagatgccca ccgatgcgtt cattgagctt catcgcccgt tcgagattga ccggcgcgcc
     1501 agcattcaac tgacccagcg tcgtcaccga cagcgtttca ggagaaattt cgaccacgaa
     1561 gttgcgctca ttcttggtga caaccgtgag gcaggtgccg ttcacactga ggctgtcacc
     1621 gatcttgaga tcacctaaga cggtcgcggc gagaatcgtc agcctagtcc ccgccaaagt
     1681 tttctccact gccgtcacgg cgcccatttc ttccacgatg ccagtgaaca tctcaatacc
     1741 ttcagctgtc agctatgact ctttcttaag aacttcgcgg accacataga caaatacgat
     1801 cagcaggaca accacaccaa tacctgctag cacgccaatc acgatgttct ctgtcgcaaa
     1861 ctctttcatc gtcgcccatt atagcaccgc gactatgccc gtctcatggt caaccagaac
     1921 actcctgccg ccagcagctg aacgcctgcg atgatggcga acgcattggc atagctcatc
     1981 cgttcgatca acaccccggc gagcagcggc ccgcttgcgt ggccgatgtc cataatggtt
     2041 ccctgcatcc ccatgccggc accaagcgtt ttgaattcag aactgtccgc gacgagcgcc
     2101 gaagaggaag acgagaccac ggcttcaccg aatccgaatc ccgccgagag gacaagcaag
     2161 aatgggaaga gcgagacatg cgggatgcag acgaaggtgg ccgcgcagat cagcaatccg
     2221 atcataatta acggctgcct ccccacacga tcggacacgc gccccatgat cggcttcgaa
     2281 aagaacgacg tgaacgcctg cacagtaaag agcaggccca cctcaccagg atttaaaccc
     2341 gcagccacgc cgtagagcgg caggaaggcc atgagcgcac cgttggcaat catcttggcc
     2401 gcatcggtcg agctggtgat caggacctta cgattcttcg caacggccgt aaagcctttc
     2461 cacatctccg ccaatagcgg cgcgaatcct ctttcgtgaa ggcgcggcgg aggcggagtt
     2521 agacgaagac tgtagaagat gacgacggcg atacatccaa agattcctgc cgtcacaaat
     2581 gccgacgaga acccagccgc atagaccaga tagccgccga tgaagggacc gagtagggca
     2641 ccggattgcg tgcaggccgt gtaagtccca agtgcggcac cgcgccgttc acgatagagc
     2701 tcagccacgg tcgccagggc gcttggggca aaaatcgccg tggcgagccc gtgaaaaaac
     2761 cgcaacgtcg tcaacacgtt caaatcggac acgaagggat agaggaaggg cggcaacccg
     2821 aaagccacga cgccaatccg taatagaagt cgccggccat agatatccga cagcgccccg
     2881 gacggcagct tgaggaacac acccgtgagg gtcgagactg acacgatcag gccgatgcgc
     2941 tccgggccgg ctcccagcga ttccgcaaac aacgccaaca ccggcatgcg cacgaggtta
     3001 tagctgatga agcagaagac cccgatggtg cagagtagaa cataactgcg tgcgggagtc
     3061 atgacggctg ggatggcaca acaaggttca gctgaatggg aaagagttgg cgctggtcct
     3121 gacgttgtcg cgcttgccga agcagtatct cgaccttcgg cgcgaccagc ccttcgaacg
     3181 ttacctgtcc atttgtcaga actgtgacgg gcttggccga atcgatcagg tgctcgttga
     3241 gaaacaggct gtaacgctgc actcgcattg tctcgacctc gatccgattc ggcgccttga
     3301 tcgacgcctc caacttggca tactgcttgc gcttgatcag ctcatcgcgc ttattgatca
     3361 gatcctccga gaaggccgcg atttgatctg tggcatcgat gcgcacccag ccgaaggcct
     3421 ggaaatgact ggcctcccgc accaatcgta tcgtggcggg aaagggatta cgacgttgcg
     3481 cattgaacca tgcgaccaga gccggcaact cttctcgtgg gaaataatgt ccgccggcca
     3541 tcggatgttc ccgttgatgc tctcgataga cgtgtggata gcctagcccc gtaagctcac
     3601 gtgaaatggt ccggcttaaa tcaacaggca tcacctgatc cttcgctccg tgaatcatat
     3661 agaccggagt cgattgaaga ttcgctagaa acggcagtag agcatcatcc agcccaccgg
     3721 ccatcggcgc aattccggca aacgtcggtg cctgatgcat cccgatcagc caggtcccga
     3781 ttccgccgtt cgacataccg gtcagaaaga tccgatcggg atcgatgtga taacgccgtt
     3841 ggaccagatg gatcgtcgcc agcactaggt cttccgcccg tctggtgaac caggctcccg
     3901 ccgggtatgt gggacaggcg agaacataat cctccccgag tctcgcctgc caccgttcca
     3961 aataagcatc gccggaaaat ccggctcc
//
LOCUS       DATDNW010000214         9790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_299097, whole genome shotgun sequence.
ACCESSION   DATDNW010000214 DATDNW010000000
VERSION     DATDNW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299097"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1575
                     /locus_tag="VI359_05355"
     CDS             100..1575
                     /locus_tag="VI359_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY6973712.1"
                     /translation="MTTHSLETLAFDNTYACLPASFYAKVNPTPFSEPPHLVSFNPVA
                     AALIDLAPEEAKRPEFAGVFGGSLLVPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     VKNKRGERWDLQLKGAGLTPFSREGDGRAVLRSTIREYLCCEAMYGLGIPTTRALCIV
                     GSDHQVYREQVETGAMLVRMAPSHVRFGTFEIFYYRKQYELLKILADYVIEQFFPHLA
                     LVGGKYAGLFNEVVERSAKLIAQWQAIGWAHGVMNTDNMSILGITLDYGPFGFMDDYD
                     PGFICNHSDHNGRYAFNQQPFIGLWNLSCLAQALLPLVPKEELKSALDRYTPIHQAFY
                     LELMRMKLGLEGARPDDESLVNDLLLLMAQSQADYTNIFRALGDFRLAPGELNEGLRD
                     YFVDRGAFDGWTARYRARLRDQTGTDAERRERMNRVNPRYVLRNYLAQTAIEKAQQKD
                     FSEIDRLLTLLQHPFSDQPGMDAYAAPPPNWGKHLAVSCSS"
     gene            1684..4941
                     /locus_tag="VI359_05360"
     CDS             1684..4941
                     /locus_tag="VI359_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973713.1"
                     /translation="MSKKPGDSKLAVAGALTLALAIAGVLLVKEPLRSSRPIGTGLEM
                     KQTTGEQLVRARPWEDPIAAVERALREAGSTRTASGATATSGSGPSLAQRLRPLRQAI
                     IERVNNGQSLTVLLVTTSGGPYVESTESRIRDRYAVGTALGVGCYAPEEEGHLSFVEW
                     EPRGAVQGVPYEWYRLRKTRICGTAGSRAESVLVVWLSDEALDRGVFATLTTFSQALV
                     CRETSKSECLLSDETRKLVRLNPSLQQAVTFKIIGPRSSSAFRALLDEAGKLYGKSHE
                     GIGVWPNADGAIDLYSPWASAMKGLLAYGLMAESGKGAACATYEDCEREFYRRLADAN
                     VRLIYDIGSDDQRFESLIAELERRQVRLGWDAVILIGEWDSFYGRALPIEFRAAACAK
                     VATFTEADLKTIQVPVDIKQWCQTVPQAVDLQIQRPADYESLTLNVFRYSYLGGLDGE
                     VPGDDAARASRAAKANAGDQSKDPTRDRPEGTSQIDYVRALVARIHDEGEGARAIGIL
                     GTDPYDALLIIKALRPAFPHAIFFTVDLDARHLHSSEYKSTRNMVIASPFGLQLEGGL
                     QRDVPPFRSSYQTSAYFAVLQAVQHVLCQAHGHQPQVGPCTTEYHVSLTPEDRLYDAG
                     AHPRLFEVGREGAVDLSIVDKEGVRTIHPLRADLAHTDEQGQLKQGIGFDDTAVAVTL
                     FLALLVGTIIAWSNQRLWLWVSKNTRLVGVVGIFLLVSFSAFVALGGAPALLSGHDEG
                     EPFSWTAGVSVWPSELIRLFVVVLCLIFLAKGSRDLSKNGDLITDDFLFQDESGFQRF
                     SPGSFWTNLKRVAHPAATMTATTVDQAWSWYREASKPAQWIMRTVLLFVLYIGMMWAL
                     GYYVLDEEYIHPCRGRLSCTVDWIMTLSSTVLVVFLNLAVFDAVMLCRRWIGWVTAST
                     GGWSQQVQEQYLVDYGLSETRKAEFEKLKYLAVVDLIGQRTEVVNRLIRYPFIALIIM
                     IAGRNDYFDIWNYPLLLLLSWALNVLLALLAAYLLYQAASQAKAAMLDGLSRHMVQAL
                     EVGQDRDVRMKQVQFIIDEVEANEKGAFVPLYQQPVIESSLYGLVALLQYLYAHQ"
     gene            4949..5668
                     /locus_tag="VI359_05365"
     CDS             4949..5668
                     /locus_tag="VI359_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973714.1"
                     /translation="MREIILGGLKTRLSGGSDGTGGGNGPVVVLLHGFGAPGDDLVPL
                     ADVINVPQGTRWMFPEGPLSLNMGFGDSRAWWIIDMARLEADRAAGRVRDLSSEVPRG
                     FPAARETLENFLAQLPRAMPIDYTKTVIGGFSQGAMLTCDIVMRTSHPFAGLVQLSGS
                     ILAKQDWRPAVGKRTGLPVFQSHGTQDPILPFVMAERLRDELIQSGLSVEWHSFRGGH
                     EIPAPVLQRLGAFLTKTLTKA"
     gene            5665..6420
                     /locus_tag="VI359_05370"
     CDS             5665..6420
                     /locus_tag="VI359_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6973715.1"
                     /translation="MSSMQAFELKGADGKLIRGDRVEGKHRQILFITGFLSKRWGNKS
                     KALAQWCQENGWGFCCYDVRGFGESEGTFTDYTLSDWISDARAVMTTLQDGPPLTVVG
                     NSLGGWIAWLMAQEFTSIERLVLIAPAFNMMGVRAKQISDERRHAWHSAGWMPWDDDP
                     VHRNYPIAWKWVEESEALWKTTFDRLRPVKTTILHGLHDTVILPVGSSQFVDRLLVCE
                     ATFPIKLKLVPGDHRLSSPEHLDLFQRSVQEKD"
     gene            6420..6587
                     /locus_tag="VI359_05375"
     CDS             6420..6587
                     /locus_tag="VI359_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973716.1"
                     /translation="MVVVIHKHCGGPAMEDSTVGEVCAIPIDRFPFSCFTCLEDIVDE
                     SELRLSEELGI"
     gene            6695..8377
                     /gene="ettA"
                     /locus_tag="VI359_05380"
     CDS             6695..8377
                     /gene="ettA"
                     /locus_tag="VI359_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY6973717.1"
                     /translation="MATNDKQVIFSLVNVGKVYPPKKQVLREIYLGFYYGAKIGVLGL
                     NGSGKSSLLKIIAGVDQNYTGEITRSKGYSVGLLEQEPQLDANKTVKEIVEEGKKELV
                     ALLHEYEAVSNKIGEAGPDEMEKLLDRQAQLQEKIEAANGWELENELEIAMDALRCPP
                     ADQKIGTLSGGEKRRVALCRLLIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGHGIPFQGNYTSWLEQKQERLEKEEKAESKRRKTLEH
                     ELEWIRMSPKARQSKGKARVNRYEELVNQKQEQRTDDLEIYIPPGPRLGDVVVEAKGI
                     SKAYDDKVLYENVNFNLPKGGIVGVIGPNGAGKTTLFKMIIGKEKPDAGSIKIGETVK
                     LGYVDQDRSLDGNKTVYEVICDGQETVKLGKVDVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLENFAGCAVISSHDRWFL
                     DRIATHILAFEGDSKVVWFEGNYSDYEADRKKRLGKEAERPHRIRYRRLTRS"
     gene            complement(8384..>9790)
                     /locus_tag="VI359_05385"
     CDS             complement(8384..>9790)
                     /locus_tag="VI359_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6973718.1"
                     /translation="PNPSTGGSLAAIAEGDAVDIDIAVRAARKAYEGVWHNVTARDRG
                     RMLYKLAQLIERDARRLAELETADNGKPIREASYIDLPQVVETFEYFAGWATKIEGET
                     IPLPGMMFNYTLREPVGVCGQIIPWNFPLLMCAWKLAPALATGNTCVLKPAEQTPVTA
                     MELAKLIQEAGFPDGVVNIVPGYGETAGAALASHPGIDKIAFTGSTEVGKLIAHAAAN
                     NLAKVSLELGGKAPNIVFADADLDQAVEGAMMGIFFNQGQVCCAGSRLFVAESVRDSF
                     LDRLQSRAKGLKIGDPLQADTQMGPQVSEEQLNRIKNYVAIGQQEGATVLTGGKSPRL
                     SGALEGGYFFQPTILVNVQNQMRVAQEEIFGPVTAVLPFKDEEDLIRQANDTIYGLSA
                     GIWTRDLGRAHRFARAVHAGTVWINTYNMIHPASPFGGYKQSGYGRELGKHALDLYTQ
                     IKSVWVDLSDKPIGWFAA"
BASE COUNT         2061 a         2737 c         2930 g         2062 t
ORIGIN      
        1 gtttggacca aggaattcga gggcgaatgg ttgacttcct cgttggacaa gaacctccat
       61 cgcttgaaag ccgttcacta gtcctgtcat gcttcgttca tgacgaccca ttcactggaa
      121 acactcgcgt ttgacaacac ctatgcctgc ctgcccgcct ccttttacgc gaaagtgaat
      181 cccactccgt tttctgaacc tccgcatctc gtgagtttca atcctgtcgc ggcagcactg
      241 atcgaccttg ctccggaaga ggccaagcgt cctgagtttg ccggtgtgtt cggaggcagc
      301 ctgttggtgc ctggcatgga gccactcgcg atgctctact ccggccatca attcggcgtg
      361 tatgttcccc aacttggcga cggccgtgcg attctgctgg gtgaagtgaa gaacaagcga
      421 ggcgaacgct gggatctgca gctcaaagga gcaggcctca ctcccttttc tcgtgaaggc
      481 gacggacgag cggtgctgcg ctcgacgatc cgagaatatc tctgttgcga ggcgatgtac
      541 gggttgggta ttcccacaac acgcgccctc tgcatcgtcg ggagcgatca ccaggtctat
      601 cgcgagcagg tcgaaaccgg tgccatgctg gtgcgcatgg ccccgtccca cgtgcggttc
      661 ggcacgttcg agattttcta ctatcggaaa cagtatgaac tgttgaagat tctggctgat
      721 tatgtgatcg aacaattttt tccgcatctt gccctcgttg gcggcaaata cgctggattg
      781 tttaatgaag tggtcgagcg gtccgcgaag ctgatcgcgc aatggcaggc gatcggttgg
      841 gcgcacggcg tgatgaatac cgacaatatg tcgattctag ggatcacgct ggactacggg
      901 ccgttcggct tcatggacga ttacgatcca ggctttatct gcaatcactc cgatcataat
      961 ggccgctatg ccttcaacca gcagcccttc atcggattgt ggaacctgag ctgccttgcc
     1021 caggcactct tgccactggt cccaaaagaa gagctgaaat cggcgctgga ccgctacaca
     1081 ccgattcatc aagcatttta tttggaactc atgcggatga agctaggtct cgaaggcgca
     1141 cgaccagacg atgaatcgct cgtgaatgat ctgctgcttc tcatggctca aagccaggcg
     1201 gactacacga atatcttccg cgcgctgggc gattttcgct tagcgccggg agagctgaac
     1261 gaaggcttgc gggactattt tgtggatcgt ggtgcgtttg atggatggac tgcccggtac
     1321 cgtgcccgcc tgcgcgacca gaccggtacg gatgccgaac ggcgggagcg catgaaccga
     1381 gtcaatccca ggtatgtctt acgcaactac ctggcccaga ccgcgattga aaaggcccag
     1441 cagaaggatt tttctgagat cgatcgatta cttaccctgc tgcaacatcc cttcagcgac
     1501 caacccggaa tggacgccta cgcggcccct cctcccaact ggggcaagca tttagcggtc
     1561 agctgctcgt cctagcaaga cctcagaccg ctcagttccc actactgaca ccgacacagc
     1621 ttgcagtccc ccacgccctc tggtgtacag tggcgggact ttccggccta gaaagcaggc
     1681 actatgtcga agaaacccgg tgattcgaaa ctcgcggtag cgggcgcact cacccttgcg
     1741 ctggctattg ccggcgtgtt gctcgtgaaa gagccgttgc gcagttcacg accgatcggc
     1801 accgggttgg agatgaaaca gaccacgggc gagcaattgg tccgcgcgcg gccatgggaa
     1861 gatcccattg ctgcggtaga acgagcccta cgtgaagcag gatcgaccag gaccgcctcc
     1921 ggtgctaccg ccacgtctgg atcgggacca tccttggccc aacggctcag accgctccgg
     1981 caagccatca tcgagcgagt taataatggg cagagcctga ccgtccttct ggtgacgacg
     2041 agcggtgggc cctacgtcga gagcaccgag tcgcggatcc gcgatcgcta cgccgtcggc
     2101 acggcgctcg gtgtagggtg ttacgccccg gaagaggagg gccacctctc ctttgtcgaa
     2161 tgggagcccc ggggcgctgt tcagggggtc ccctatgaat ggtatcggct caggaagacg
     2221 cgaatttgtg ggacggcagg atcgcgtgcg gagagcgtcc tcgtcgtctg gttgtctgac
     2281 gaagcccttg accgaggggt ctttgccacg ctgacgacgt tttctcaagc gctcgtctgc
     2341 cgggaaacca gcaagagcga atgcctgctc tccgacgaga cacgcaaact ggtgcggctg
     2401 aacccctcgt tgcagcaggc ggttacgttc aaaattatag gcccccgcag ttcgtcggca
     2461 tttcgcgcgc tcctggatga ggcagggaag ctgtacggca agtcgcatga agggatcggt
     2521 gtctggccga atgctgacgg cgcgatcgac ctgtattccc cttgggccag tgcgatgaag
     2581 gggctgctcg cctacgggct catggcggag tccggcaaag gagctgcctg cgcgacctac
     2641 gaggattgcg aacgcgagtt ttatcgccgg ctggccgatg cgaacgtgcg gctcatctac
     2701 gatattggat ccgacgatca acggttcgaa tccctgatcg cggaattgga acgacgacag
     2761 gtgcggctcg gctgggatgc tgtcattctc atcggcgaat gggattcgtt ctatggacgg
     2821 gctctgccca tcgaattccg cgccgctgcc tgcgcgaaag tggcgacctt cacggaagcc
     2881 gatctcaaga cgattcaggt gccggtcgac atcaaacagt ggtgtcagac tgtgccgcag
     2941 gcggtcgatc tgcagatcca acggcccgcc gattacgaat cgctcacact caacgtcttt
     3001 cgctatagct atctcggtgg cttggatgga gaagtgcccg gcgacgacgc ggcgagagcc
     3061 agtcgagccg caaaggcaaa tgccggggac cagtccaaag atccgacgcg ggatcgcccg
     3121 gaggggacca gccagatcga ttacgtgcgc gcgctggtcg cccgcattca cgacgagggg
     3181 gaaggcgcaa gggccatcgg cattctgggc acagatccct acgacgcctt gctgattatc
     3241 aaagccctcc gcccggcgtt cccgcacgcg attttcttta ccgtcgatct cgacgcgcgc
     3301 catctccact cgagcgaata caagtcgacg cgtaacatgg tgatcgcctc tccgttcggg
     3361 ttgcaattag agggaggcct tcagcgcgat gttccaccgt tccgcagcag ctatcaaacg
     3421 tcggcttact ttgccgtttt gcaagccgtg caacacgtcc tctgccaggc gcacggccat
     3481 caaccgcagg tcgggccttg cacgaccgaa tatcacgttt cgctgacgcc tgaagatcgt
     3541 ctctatgacg caggtgctca tccgaggcta ttcgaagtcg ggcgagaggg cgccgtagac
     3601 ttgagcatcg tggacaagga aggggtgcga accatccatc ctctccgggc ggatcttgcg
     3661 cacaccgatg aacaagggca gctcaaacag gggattggat tcgatgatac agccgtcgcg
     3721 gttaccttgt tccttgcgct gctcgtcgga acgatcatcg cttggagcaa tcagcgtctc
     3781 tggctgtggg tatcgaagaa tacccgactg gtgggagtcg ttgggatttt cctcttggtc
     3841 tcattctcgg ccttcgtcgc attaggcggt gcgcctgcac tgttgtccgg ccacgatgaa
     3901 ggcgagccgt tctcctggac tgcgggagtt agtgtctggc cgagcgagtt gattcgtcta
     3961 ttcgttgttg tgctctgtct gatcttcctt gcaaaaggat cgcgcgacct ctctaagaac
     4021 ggcgacctca tcacggatga cttcctcttc caagacgagt ccggctttca acgtttttca
     4081 cccgggagct tctggaccaa cctgaagcgg gtcgcccatc cggccgcgac gatgaccgcg
     4141 acgacggtgg atcaggcctg gtcgtggtat cgggaggcga gcaagccggc acagtggatc
     4201 atgcggaccg tcctgttgtt tgttctctat atcgggatga tgtgggcatt gggctactat
     4261 gtgctggatg aggagtacat ccatccctgc cggggtcgac tgagctgcac ggtggattgg
     4321 atcatgacgc tgagcagcac ggtcttggtg gtgtttctca atcttgcggt gtttgatgcc
     4381 gtgatgttgt gtcgccgctg gatcggctgg gtgaccgcgt cgacgggcgg ctggtcgcaa
     4441 caagtgcaag agcaatatct cgtcgattac ggcttgagcg agacccggaa agccgagttc
     4501 gagaagctga agtacctcgc cgtagttgat ttgatcgggc agcggaccga agtggtgaat
     4561 cgcctgatcc ggtatccctt tattgctctg atcatcatga ttgcgggacg gaacgactac
     4621 ttcgatatct ggaattatcc gctcctttta ctgctctcct gggcgctgaa cgtgttgctg
     4681 gctttgctgg ctgcgtatct gctgtaccag gcagcgagcc aggcgaaagc cgccatgctc
     4741 gatgggctga gtcggcacat ggtccaggcc ttggaagtcg gccaggatcg cgatgtccgg
     4801 atgaagcagg tccagttcat catcgacgag gttgaagcca acgagaaggg tgccttcgtg
     4861 ccactctacc agcaaccggt gatcgagtct tccctctacg gcctggtggc actgctccaa
     4921 tatttgtacg cccatcaata agctgtccat gcgagaaatc atcctgggtg gcttgaaaac
     4981 acggcttagc ggcggcagcg acggcacagg cgggggcaac ggcccggttg tcgttctcct
     5041 gcatgggttt ggtgcgccag gggacgatct cgttcccctc gccgatgtaa tcaatgtgcc
     5101 gcaggggaca cgttggatgt tcccggaagg accgttgtcg ctgaatatgg gattcgggga
     5161 ttcgcgggcc tggtggatta tcgacatggc ccggcttgaa gcggatcggg ccgcggggcg
     5221 cgtgcgagat ctgtcgagcg aagtgccgcg cggcttccct gccgcgcggg agacgctcga
     5281 gaactttctc gcgcagcttc cgcgcgcgat gccgatcgat tacacaaaaa cggtgatcgg
     5341 gggattttcg cagggcgcca tgttgacctg cgacatcgtc atgcgcacct cgcatccgtt
     5401 cgccgggctt gtccagctct caggatcgat cctggcgaag caggattggc gtcccgctgt
     5461 cggtaaacga acagggctgc cggtctttca gagccatggc acacaggacc cgattctgcc
     5521 ctttgtcatg gccgaacggc tgcgtgacga attgatccag agcggattgt cggtcgaatg
     5581 gcacagcttt cgcggggggc acgagattcc tgcgccggtc cttcaacggc tgggcgcatt
     5641 cttaaccaag acattgacga aagcatgagt tcgatgcagg cgttcgaact gaaaggcgca
     5701 gatggaaagc tgatccgtgg cgatcgggtc gaaggcaagc atcgacagat tctcttcatt
     5761 accggctttc tctccaaacg atgggggaac aagagcaagg cgctggcgca atggtgccag
     5821 gagaacgggt gggggttctg ttgctatgac gtgcgagggt tcggcgagtc cgagggaacg
     5881 ttcacagact acacgctgtc tgattggatc agcgacgcac gagccgttat gacaacgctg
     5941 caagatggtc cgcccttgac tgtggtgggc aactcgttgg gtggatggat cgcctggctg
     6001 atggcgcaag agttcacgag catcgaacgg cttgtcctca ttgcacccgc gtttaacatg
     6061 atgggggtgc gggccaagca gatttccgat gagcggcgcc atgcctggca ctcggcggga
     6121 tggatgccgt gggacgacga ccccgtccat cgtaactatc cgattgcgtg gaagtgggta
     6181 gaagagagtg aagccttgtg gaagacgacg ttcgatcggc tccggcccgt taagacaacg
     6241 atcctccacg gattgcacga tacggtgatc cttcctgttg gcagcagcca gtttgtcgac
     6301 cggctcctgg tttgcgaggc cacgtttccc atcaagctga aattagttcc gggcgaccat
     6361 cgtctgagca gtcctgaaca tcttgacctg tttcagcgat cggtccagga gaaagactga
     6421 tggtcgtggt gatccataaa cactgtggtg ggccggcgat ggaggactcg acggtcggcg
     6481 aggtttgcgc gatccccatc gaccggtttc cgttttcgtg cttcacctgc ctcgaagaca
     6541 tcgtcgacga atcggaactc cgactgtccg aagaactggg aatctagttc ccgcccgcaa
     6601 ggtgccgtct cctccgtccc ctttatgttg tgaaacgtcc gcagcttttg ttatggtggg
     6661 ccctcctttc ccctatcaac gaaccagagg acctatggct acgaacgata agcaggtgat
     6721 tttttcactc gtcaatgtcg gcaaagtcta tccacccaag aaacaagtgc tgcgagaaat
     6781 ttatctcggc ttttactacg gcgccaaaat cggcgtgctg gggttgaatg ggtcggggaa
     6841 gagcagcctc ctcaagatca tcgccggagt ggatcagaac tacaccggcg agatcacacg
     6901 atcgaagggc tattcggtcg gtttgctcga gcaggaaccg cagcttgatg cgaacaagac
     6961 ggtgaaagaa attgtcgaag aaggcaagaa agaactcgtg gccctcttgc acgaatacga
     7021 agctgtcagc aacaagatcg gtgaagcagg tcccgacgaa atggaaaagc ttctcgatag
     7081 gcaggcacaa ctgcaggaga aaatcgaagc ggcgaacgga tgggaactgg aaaacgaatt
     7141 agagatcgcg atggacgcgc tgcggtgccc tccagcggat cagaagatcg ggacgctttc
     7201 cggaggcgaa aagcgccgcg tggcgctctg ccgtctcctg attcaggaac ccgacattct
     7261 gctgctcgac gaaccgacca atcacttgga cgctgaatcg gtccaatggc tcgagcaaca
     7321 cctgcaacag tataaaggca ccgtcatcgc cgtgacacac gatcggtatt ttctcgacaa
     7381 tgtagccgga tggatactcg agctcgatcg tggtcacggc attccgtttc aaggtaacta
     7441 cacgtcctgg ttggaacaga agcaggagcg gctcgagaaa gaggagaaag ccgaatccaa
     7501 gcggcgcaaa acgctcgaac acgaactgga atggattcgc atgtcgccca aagcccggca
     7561 gtcgaagggc aaggcgcgag tgaatcgcta cgaagaactc gtaaaccaga aacaagaaca
     7621 gcggaccgat gacctggaaa tctatattcc gccgggaccc agactcggtg acgtggtcgt
     7681 ggaagcgaag gggatcagta aagcctacga cgacaaggtg ctctatgaga acgtgaactt
     7741 taatctgccg aagggcggga ttgttggggt gatcggtccg aacggtgcag ggaagacgac
     7801 cctctttaag atgatcatcg gaaaagagaa gccggatgcc ggttcaatca agatcggcga
     7861 aacggtcaag ctcggttacg tcgatcagga tcggagtttg gatgggaaca agaccgtcta
     7921 cgaggtgatt tgcgacgggc aagaaaccgt gaagttaggc aaggttgacg tcaacgctcg
     7981 cggctactgc gcacggttca actttgccgg cacagatcag caaaagaaag tcaaagacct
     8041 gtcaggcggc gaacgcaatc gcgtccacct cgcccggatg ttgaaggaag gcgcgaacct
     8101 catcattctc gatgagccga caaacgatct cgatgtcaac accctgcgcg cgctggaaga
     8161 gggactcgag aatttcgccg gctgcgccgt catcagcagc catgaccggt ggttcctcga
     8221 ccggatcgcg acccatattc tcgctttcga aggtgacagt aaggtcgtgt ggttcgaagg
     8281 gaactacagc gactacgaag cagatcgcaa aaagcgcctc ggcaaagaag ccgaacggcc
     8341 gcaccgcatc cggtatcgta gattgacgcg cagctgaacc gagtcatgcc gcaaaccagc
     8401 cgatcggctt atccgaaaga tcgacccaca cactcttgat ctgcgtgtac aggtcaagtg
     8461 catgtttgcc cagctctcgt ccataaccgg actgcttata gccgccgaag ggagaggctg
     8521 ggtgaatcat gttgtacgta ttgatccaaa ccgtgccggc atgcaccgct ctggcaaacc
     8581 gatgggcacg tccgaggtct cgggtccaga ttccggcaga caggccataa atggtgtcgt
     8641 tggcctgtcg gatcagatcc tcttcgtcct tgaacggaag aaccgccgtg accggcccga
     8701 agatttcttc ttgggccacg cgcatctgat tctgcacgtt gaccaggatc gtgggctgaa
     8761 aaaaatatcc cccttcgagt gcaccggaaa gacgaggcga tttcccgccg gtgagcacag
     8821 ttgctccctc ctgctggcca attgcgacat agttcttaat ccgattcagt tgctcctcgg
     8881 acacttgcgg ccccatctgt gtgtcggcct gcagaggatc gccgattttg aggcctttcg
     8941 ctctcgattg gagccgatcg aggaatgagt cccgcaccga ttccgcgacg aagaggcgcg
     9001 agccggcgca acagacttgg ccttgattga agaagatgcc catcatggcc ccttcaaccg
     9061 cctgatcgag atcagcgtcg gcaaaaacga tgttcggcgc tttgccgccg agctcaagcg
     9121 aaaccttcgc tagattgttt gcggccgcat gggcgatcag ttttcccact tccgttgagc
     9181 cggtgaacgc gatcttgtct atgcctggat gggaagccag cgccgcccca gccgtttcac
     9241 cgtaaccggg cacgatattg accacgccgt ccgggaatcc ggcttcttgg atcagtttgg
     9301 ctaactccat ggctgtgaca ggtgtttgtt ccgccggctt gagcacgcac gtattacctg
     9361 tagcgagagc aggcgcaagt ttccaggcgc acatcaacaa aggaaaattc caggggatga
     9421 tctgtccgca cacgccgacc ggctcccgca acgtgtaatt gaacatcatt ccggggagcg
     9481 gaatcgtttc cccttcgatc ttcgtggccc aaccggcaaa gtattcgaac gtctcgacca
     9541 cttgtggaag atcgatgtaa cttgcttcgc gaattggttt gccgttatcc gcggtttcca
     9601 gctcggccaa ccggcgtgcg tcacgctcga tcaactgggc cagcttgtac aacatgcgcc
     9661 cacggtcccg cgcggtcacg ttgtgccaga ctccctcata ggccttgcgc gcagcgcgta
     9721 cggcgatatc gatgtcgacc gcgtcgcctt ctgcgattgc cgccagcgat ccgccggtgg
     9781 acggatttgg
//
LOCUS       DATDNW010000215         5341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_573003, whole genome shotgun sequence.
ACCESSION   DATDNW010000215 DATDNW010000000
VERSION     DATDNW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573003"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VI359_05390"
     CDS             <1..657
                     /locus_tag="VI359_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6973719.1"
                     /translation="VVPNIPGSAPLPSSLIFLYLILTITGIVIFATLSGESKDAFWGP
                     IQRFLTGENIGALQTARYAVLILFPLLVGWQTYGSTAQSDAPPAENRTIHPAPPGEYT
                     GLSNPVAKTPDNIMQGKGFYAAFCSPCHGGNFDGKGPASRGFNPPPANFSDPTTIAML
                     QESYLFWRIKKGGVGLPIEGMPWKSAMPRWEVELPDEWIWKIIMGEYDGAHQSPRTWE
                     "
     gene            688..1521
                     /locus_tag="VI359_05395"
     CDS             688..1521
                     /locus_tag="VI359_05395"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEY6973720.1"
                     /translation="MTRGMHQQMKWGGAIAGMGAACLVAGTTIALAQESVAVRATMVK
                     GTIPADDPNAAVWNSAPQAQFPMSPQVHWQNRIQEATVKDLKVRALHDGNQLAVLLEY
                     ADPTEDPDDAAALEFMVGDKKAHFAHGQPMAQVEGGPVNIWFWKNKENKAVDMNAKGF
                     GTLKAQEHQDVKAKGVYANGMWKVVFSRSLANEHVNEDVQVKPGEFINIAFAVWDGKK
                     DASGELREKGSQKAVSSWWYFRAEAPPDYSGYLYAALAVGLALGFQFVLIRKLKKGKS
                     A"
     gene            1518..2537
                     /locus_tag="VI359_05400"
     CDS             1518..2537
                     /locus_tag="VI359_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY6973721.1"
                     /translation="MKAARLHVLGAVAGLVGVWAMVAGGCANEQQRKGHELYTHYCMH
                     CHGENGRQNEGFNWASMPDPKPKDLSNKSEMSTFKDEDIFNTVSRDMKDTSPGGDKIG
                     DDDFAVPTMPTFKYTLSEEEIWAIVGYVRTLHGMKLEFKVEERKKELADALQAAQAKF
                     DQAKQAYDDAEKKASEEAEKKSEALKKDVEVDESVYAKEQAAMVQAKKDLDAAQAALN
                     YFSTRPGKGLNIARPDLTMKPEQAAQLAAVGKRLYANKYGCNGCHSVGGEGGKVGPAL
                     DRAGFRLNGTWIYRWVRNPQAMKPETRMPALGVSDADAKAVAMYLNTLRAPKPDDTPV
                     AKPAS"
     gene            complement(2540..3439)
                     /gene="ubiA"
                     /locus_tag="VI359_05405"
     CDS             complement(2540..3439)
                     /gene="ubiA"
                     /locus_tag="VI359_05405"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY6973722.1"
                     /translation="MSGSWPTDSASKAPTIPSLSACGRLIRLQNQTGTLLLLLPTLWA
                     LVLASRGLPPWRLTVIFVIGSFLMRSAGVVLNDLADRSLDRHVARTQSRPLASGELAP
                     GHAVAILVVLVTLAAALLLLLDPMTIMLSPIALLLAALYPFAKRVIHIPQAMLGLAFG
                     WGTIMAWSAAQGTVAAPAWCAFAATLCWAIGYDTIYALQDQDDDRRIGVKSSAIFFGS
                     LAWLAVGVALGGMLLLLSIAGWLTQVGWFYYLVLIAVGFFCVWQVRRLRHTISASIAL
                     SMFQQHVWVGTAVLGGLLGGFLL"
     gene            3599..>5341
                     /locus_tag="VI359_05410"
     CDS             3599..>5341
                     /locus_tag="VI359_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY6973723.1"
                     /translation="MVLSSLAAGCSLFQSRNGLKTGYYLPLVAQLREAASVKGAQITY
                     KDACDQPQSFPIGDLLQEAIKRKTGLVFEKVVPTEKGSTPAAVDGYVDVAVGFNRMEL
                     VIYRKAKRSYPATMALGLDFAYTDAEGTVLVQKKLQSSSTGEVDVISEGSCQVKGIEP
                     IIRDVVETVTDGMAKHLGESTKVREQVAARKAGAPRPTSPANPPPLAVQGSTDVPPPI
                     LPTAPNPAEATRVPAPTPPDEATTLTFRAIVRDQNRNLVLHGGEAIAVEVEVKNEGPR
                     VVHGVEIQVGGTPALTEHLSALITVGELRSGEVKRVTLDGTIGAVREPIQAELILTLR
                     AGSPAVKIPSSKKFVVAMKPESDAEAALLPVDVDQLPKRAGKLKQPKAVGITIGVEQF
                     RDTGMSRIRYAARDAEVMAMYLKSIVGIPADRVRTLVDSQALKQDVAELLEEWLPRHV
                     DPTTVVYVYYSGLGVVDGTTGAVSLVPFDGPASTNSRVYSLRRLQEALIKLPVERAIV
                     MLDISLEPAAGADPAAKVAPIWEPEGSPKDRLMWMVGNRSVQESHRYDPGQHGLFTYQ
                     LLKGLGGGADIDKDG"
BASE COUNT         1248 a         1444 c         1659 g          990 t
ORIGIN      
        1 gtcgtgccca acatccctgg gtccgcaccg ctcccgtcca gcctgatttt tctctatctg
       61 atactcacga ttacaggcat cgtgatcttc gccacgttga gcggagaatc gaaggacgca
      121 ttctgggggc cgatccagcg atttttgacc ggagaaaata tcggggcgtt gcagacagcc
      181 cgctatgcgg tgttgattct gttcccgctt ttggtgggat ggcaaacgta tgggagtacg
      241 gcgcagagcg acgcaccgcc cgctgaaaat cggaccattc acccggctcc tccgggagag
      301 tataccggcc tgtccaatcc agtggccaag acccccgaca acatcatgca gggcaaaggg
      361 ttctatgccg ccttctgctc gccgtgccat ggaggaaatt tcgacgggaa gggcccggct
      421 tcgcgagggt tcaatcctcc tccggcgaac ttctcggacc ccacgacgat tgccatgctg
      481 caggaaagct atctgttctg gcgcattaag aaaggcggcg ttggactgcc gatcgagggc
      541 atgccttgga aatccgccat gccgcgttgg gaagtcgagt tgccggatga atggatctgg
      601 aaaattatca tgggtgagta cgacggcgcg caccaatcgc ctcggacctg ggaatagcac
      661 gaaattcgaa ctcgtaacga ggctcgtatg acacggggaa tgcaccaaca gatgaaatgg
      721 ggcggcgcaa tcgcggggat gggggcggca tgcttggttg ccggaacgac gatcgccctg
      781 gcgcaggaaa gcgtcgctgt gcgcgccacc atggtgaagg ggacgattcc agccgacgat
      841 cccaatgcag ccgtgtggaa cagcgcgcca caagcgcaat tccctatgtc gccacaggtc
      901 cattggcaaa atcgcattca ggaagccact gtcaaggacc tcaaagttcg tgctctccac
      961 gatggcaatc agctggccgt gctcttggaa tatgccgatc cgaccgaaga ccccgatgat
     1021 gcggcggcgc tcgaattcat ggttggagac aaaaaagccc actttgcgca tggccagccg
     1081 atggcgcaag tggaaggcgg cccggtcaat atctggtttt ggaagaataa agagaataaa
     1141 gcggtcgata tgaacgccaa aggtttcggc accctcaagg cgcaggagca tcaggacgtg
     1201 aaagccaagg gtgtctatgc gaacgggatg tggaaagtgg tgttctcccg cagcctggcg
     1261 aacgagcatg tcaatgaaga tgtgcaggta aaacccggcg aattcatcaa catcgccttt
     1321 gccgtgtggg atggaaaaaa agacgccagt ggagaactca gagaaaaagg ttcccagaaa
     1381 gccgtgtcct cctggtggta tttccgcgcg gaggccccgc ctgattattc agggtatctt
     1441 tatgcggctc ttgccgtcgg gttagcgctg ggatttcaat tcgtcctgat ccgcaagctc
     1501 aagaaaggga agtcagcatg aaggccgcac gacttcacgt attgggagcg gtggctggcc
     1561 tggtcggcgt gtgggccatg gtcgccggag gctgtgccaa tgagcagcag agaaaagggc
     1621 atgaactcta tactcactat tgtatgcact gtcacggcga aaacggacgt cagaacgagg
     1681 ggttcaattg ggcgtcgatg ccggacccca agcccaagga tctctccaat aagtccgaga
     1741 tgtcgacgtt caaggacgag gatatcttca ataccgtttc gcgtgacatg aaagacacaa
     1801 gccctggtgg cgacaagatt ggcgacgatg actttgccgt ccccaccatg ccgaccttca
     1861 aatacacgct ctctgaagaa gaaatctggg ccattgtcgg ctatgtgcgg acactgcacg
     1921 ggatgaaact tgagtttaag gtcgaggaac gcaagaagga actcgcggat gccctccagg
     1981 ccgcccaagc caagttcgat caggcgaagc aggcctacga cgacgctgag aagaaggcca
     2041 gtgaagaagc ggaaaagaag agtgaagcgc taaagaagga cgtcgaggtc gatgaatcgg
     2101 tctatgcgaa agagcaggca gctatggtcc aggcgaagaa agatctcgat gccgcccaag
     2161 ctgcgctcaa ttatttctcg acgaggcccg gcaaaggcct gaacattgcc aggccggatc
     2221 tgacgatgaa gccagaacag gctgcgcagc tcgcggcagt cggcaagcgg ctctacgcca
     2281 acaaatacgg ctgcaacggc tgtcatagtg tcggtggcga gggcggcaag gtcggaccgg
     2341 cgcttgatcg agccggtttt agattgaacg gcacctggat ctatcgttgg gtgagaaatc
     2401 cgcaggccat gaagccggag acgcgaatgc cagccctcgg ggtcagcgac gcagatgcga
     2461 aggctgtggc aatgtatctg aatacgttgc gcgcgccgaa gcctgacgac acgccggttg
     2521 ccaagccggc gagttgagtt tatagtaaaa agccgccgag cagtccgccc agcacagcgg
     2581 tcccgaccca tacgtgttgc tggaacatgg acagagcgat tgacgccgag atggtgtgtc
     2641 tgagtcggcg tacctgccag acacaaaaga aaccgacggc gatcaatacc agatagtaga
     2701 accacccgac ttgtgtgagc catcctgcaa tgctcagcag taggagcatc cctccgagcg
     2761 ctacaccgac cgcgagccat gccaacgagc cgaagaaaat ggccgatgat ttaacgccga
     2821 tgcgacggtc gtcgtcctgg tcctgtaacg catagatcgt atcgtagccg attgcccagc
     2881 acagcgtggc cgcaaaggca caccaggctg gagccgccac ggtcccctgc gcagccgacc
     2941 acgccatgat cgttccccac ccgaatgcga ggcccaacat ggcttgggga atgtggatga
     3001 cccgcttggc aaaggggtac agtgcagcca agagaagcgc gatggggctc agcataatgg
     3061 tcattggatc gagcaacagc aacagcgcgg cggccagtgt gacgaggacc actaggatgg
     3121 caaccgcatg gcctggcgca agttcaccgg aagcaagcgg acgagactgc gtgcgtgcca
     3181 cgtgccgatc gagcgaacga tcggcaagat cgttgagtac cacaccggcg ctgcgcatga
     3241 gaaatgagcc gatgacgaag atgaccgtca atcgccaggg tgggagacca cgggacgcga
     3301 gcaccagcgc ccaaagcgtc ggtaacaaca ggagcaacgt gccggtctgg ttttgtagtc
     3361 ggatgagccg gccgcatgca gaaagtgatg ggattgtggg tgctttcgag gccgaatccg
     3421 ttggccaaga cccggacatg ggccagacct tagccgacgg ggatttccca agtcaatcgc
     3481 atgaatggtc aagaatcgtt gtgatgggcc gatgcttttc ggtataacca tacgtgtgca
     3541 ggtgggtgat ctagtgcctc caatccttcg ttgcagcgcc tccatagggg ttgtagcggt
     3601 ggtgctgtct tcgctggcgg ctggctgctc tctgtttcaa agtcgaaatg ggctcaaaac
     3661 agggtactac ctccccctgg tcgcgcagct gcgtgaggct gccagtgtca agggggcaca
     3721 gataacctat aaggatgcct gcgaccagcc tcaatctttc cctatcggag atctcttgca
     3781 agaggcgatc aaacgcaaga cgggacttgt gttcgagaaa gtggtgccta ctgaaaaggg
     3841 atcgactccg gcggcagtgg atggctatgt cgatgtcgca gtgggcttca atcgtatgga
     3901 actggtcatc tatcgaaagg ccaaacggag ctacccggcc acgatggcac tcgggctgga
     3961 ttttgcgtac accgatgccg aaggcaccgt gttagttcag aagaagctcc agagctcaag
     4021 caccggagag gtcgacgtca tatccgaggg aagttgccag gtcaaaggga tcgagccaat
     4081 tatccgggac gtggttgaga ccgtcaccga cgggatggcc aaacacctgg gtgagtcgac
     4141 aaaggttcga gagcaggttg cggcgagaaa ggctggagca cctcggccga cctcaccggc
     4201 gaacccgcca cccctggcag tgcagggttc gacggatgtt ccgccgccga ttcttcccac
     4261 ggcgccaaat ccagctgagg ctacgcgtgt gccggcgccg actcctcccg atgaagccac
     4321 aacgttgacg ttccgggcga tcgtacgcga tcaaaatcgc aacctcgtcc tgcatggtgg
     4381 tgaagcgatc gccgtggagg ttgaggtgaa aaacgaaggc cctcgtgtgg tgcatggcgt
     4441 ggaaatccag gtcggcggca cgccggctct gaccgaacat ctttccgccc tcatcacggt
     4501 cggggagttg cggtcaggtg aagtcaagcg cgtcacgttg gatgggacga tcggggcagt
     4561 cagagaaccg atccaggcgg aactcattct gacattgcgg gcgggctcgc ctgcggtaaa
     4621 aataccttct tcaaagaagt tcgtcgtggc gatgaaaccg gaatccgatg cggaggcggc
     4681 tctactcccg gtcgatgtcg atcaattacc gaagcgggcc gggaagctca agcaaccgaa
     4741 agcggtgggc attaccatcg gcgtagagca attccgtgat accggcatgt cccgaattag
     4801 atatgccgcc cgcgatgctg aagtcatggc tatgtatctc aagtcgattg tgggcatccc
     4861 cgctgatcgc gtccgtacgt tagtggattc gcaggcgttg aaacaggatg tggcggaact
     4921 cctggaagaa tggcttccga ggcacgtcga tccaaccacc gtcgtgtatg tctactactc
     4981 cggactgggg gtcgttgatg gaacgacagg agcggtctcg ctagttccgt tcgacggccc
     5041 ggcttcgacg aactcacgcg tgtactcact ccgtcgattg caggaggccc tgatcaaact
     5101 gcctgtcgag cgggcgatcg tgatgttgga catctcgctc gaaccggcgg caggtgcgga
     5161 tcctgccgcg aaagtggcgc cgatatggga gccggaaggc tcgccaaaag accgactcat
     5221 gtggatggtc ggcaaccgca gcgtccagga gtcgcatcgc tacgatcccg gacaacacgg
     5281 cctgttcacc tatcaactcc tcaaggggct cggaggcggc gccgatatcg ataaggatgg
     5341 a
//
LOCUS       DATDNW010000216         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1058955, whole genome shotgun sequence.
ACCESSION   DATDNW010000216 DATDNW010000000
VERSION     DATDNW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058955"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            776..1348
                     /locus_tag="VI359_05415"
     CDS             776..1348
                     /locus_tag="VI359_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6973724.1"
                     /translation="MMYARKIPVPIWALAGLVVELQGCHLGPLPSVPDLGGTYISQSA
                     TIQADNKTVDEILTAFKQAEAAVQRRDLDALMALYAEGYKHRGFTKDSLRTEWKHLFD
                     NYHDFSSTHVLTGIAVESDKSPATAQIICTGSLWAISNDTNQRVNIDSWYGEVHYLIY
                     VDGAWRMRGHAWEVLVPKDPRAAHPPHPFF"
     gene            complement(1763..2863)
                     /locus_tag="VI359_05420"
     CDS             complement(1763..2863)
                     /locus_tag="VI359_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973725.1"
                     /translation="MKSRRTQTKSCSPILHYGVVSLPAVTRAVLSMWVGLVLCSPVLE
                     ASALPTAEELLQELQLSDSDRQSIREGKIVTWSATEGSDRELALGMAMRAKTKTENLV
                     ALFREAAAFKNVSAIIAYGKIVGEGTLADFAGVKLAPNGEKEARRYLEAEPGDDLNLD
                     AKEIAAFRALKSASKDGAVPIEKVETLIREGLLARYQAYHSKGLAGIAPYARKNGHQT
                     LASDELSIATKQSKLVAKYLPSVYNVLLNYPAIKFKEAEELEEQFFWVTIEVFGRPTY
                     VLSHRLRFRIGESYVVVDRHYYASHDYNSLQQGAVALPSKDDLLVTYLSRVSTDQVAG
                     FGSSAKHPVSRALMAPYLKDLLEALRAKTEKQ"
     gene            complement(3059..3134)
                     /locus_tag="VI359_05425"
     tRNA            complement(3059..3134)
                     /locus_tag="VI359_05425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3099..3101),aa:Ala,seq:cgc)
     gene            complement(3309..3983)
                     /gene="purN"
                     /locus_tag="VI359_05430"
     CDS             complement(3309..3983)
                     /gene="purN"
                     /locus_tag="VI359_05430"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY6973726.1"
                     /translation="MSTKRPTPLRVAVLASGRGSNLQAVIDAVEAKQVDAHIAAVISN
                     KKDALALERARKHSLNDLFVDPKPFAGRPDSREAYDGELLKVLQHHEVELVLLAGYMK
                     IVTAVLVNAYANRIMNIHPSLLPAFPGLDVQKKAIDWGCKLAGCTVHFVTEGVDEGPI
                     ILQAAVPVLDHDTPEMLAARILDQEHKIYPRAVQLFAEGRLRVEGRRVFIDAAKPEGQ
                     AIISPS"
     gene            complement(3973..>4300)
                     /locus_tag="VI359_05435"
     CDS             complement(3973..>4300)
                     /locus_tag="VI359_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY6973727.1"
                     /translation="CPIKGIAHITGGGITENLPRVFPPGVRAQVRRSAWKVPPIFDVI
                     AKLGAVARDEMYRVFNMGIGLILVVPSDAANAVIQRATALGDRAHRIGRIVSANDEKP
                     LVEYVD"
BASE COUNT          913 a         1238 c         1194 g          955 t
ORIGIN      
        1 ccaggttggt cctggcttca gcttggcggg cccgcgcctg atacgccaag aagttcggga
       61 tggcgattgc cgccaagatc ccgatgatcg ccactacgat catcaactcg atcaacgtaa
      121 aacccttttg ttccttcaac tgcttataca ttgctgctcc ttattctatt gtagatagtt
      181 gactgcccgg ccttttggtt gctcagttgt cctccggcaa ccgaagcgct cgtacacagt
      241 cctaccttta cgcaggttcc gtacctggaa tactcgacca gagaaagaga aaagactgaa
      301 tatgaaagca acgggttagc gaacagcgct gctaggacac agaatcaatg ccaacgaagt
      361 cttacgtttt cggccttatc tgccaagatt tggtcccagt gacgattgcg cccgcaagcg
      421 atccaaccgt cgtgcggtcc cctcctccgc tttgaagttc aaggggcagc ccctagcggc
      481 ctagacgcgc ggctccgcgg tttggggaac cgtttccttg accctcctca ggcctactca
      541 ggctcacgca ggcccactcc agccaaggtt gcgcgcgaaa cccgaacttt ggctggagtt
      601 gagcacaaaa tgagtacaac cagatctcgg gaagcgtgag tttctccgtc caactggaaa
      661 ctgagcggga gtgttgacgg ccttataggg taggcttagg atcttaacaa aatggaagct
      721 tcgcattcgg aggcctggaa ttgggggcgt tgagaataat ttgagagaga gcctcatgat
      781 gtacgcgagg aaaatacccg ttccgatctg ggctttagcc ggacttgtcg tagagcttca
      841 aggctgtcat ttgggccctc tgccctctgt cccggatctc ggtgggacct acatttctca
      901 atccgcgacc atccaagccg acaacaaaac ggtggacgag atcctcactg cgtttaagca
      961 ggccgaggcg gccgtccagc gccgagacct cgatgcgttg atggccctgt atgcggaagg
     1021 ttacaagcat cgtggcttca cgaaagacag cttgaggact gaatggaagc acctctttga
     1081 caactatcat gacttctcca gtacccatgt cctaacaggc attgcggtcg agtcggacaa
     1141 atctcccgcg actgctcaga tcatctgcac cggaagtctt tgggcgattt ccaacgacac
     1201 caaccagcga gtcaacatcg atagttggta tggagaggtc cactatctca tttacgtgga
     1261 tggggcttgg cgcatgcgag gacatgcgtg ggaagttctt gtccccaagg acccgcgcgc
     1321 tgcgcatcca cctcatccat ttttttgagg tgcctctgtg gtctcaccga caggcactct
     1381 tttttccgat gaaagcgaat tcataaacat cgggagctgg tcggattaaa agggttggaa
     1441 atcaaggcga cagtgggcag ctattgggag gttcaaaagc aactgatttt gagaaggcct
     1501 ggaggggcct gggcaccgta gggtcttcgt tccatttgcc cctggccgca cccggcctcc
     1561 gtcgccctct tgggcagtga ttgattttcc ggaggcgggg aatttcactc tgtgttcatt
     1621 taggcccatc tgcaccaaga gcctccttct ttccgatcac actcaccttc tccggggcac
     1681 cagacccgag caatggcgca gtcagctgaa tctcccgctg tcggggcatc aggattgctg
     1741 atgggcctga gtcagcggcg cgctactgct tctctgtctt ggcccgaagg gcctcaagca
     1801 agtccttgag gtatggcgcc atcagggcac gagatacggg atgcttcgcc gaggatccaa
     1861 atccggcaac ctggtcggtg gatacacggc taaggtaggt cacaaggaga tcgtccttcg
     1921 atgggagtgc caccgcccct tgctgcagac tgttgtaatc atggctcgcg tagtagtggc
     1981 gatcaacgac cacataggac tctccgattc ggaagcgcag gcggtgagac agtacgtagg
     2041 tcggacgccc aaagacttcg atagtcaccc aaaaaaactg ttcctcgagc tcttcggctt
     2101 ctttgaactt gatggccgga tagttgagca gcacgttgta cacggaggga aggtacttgg
     2161 cgacgagctt cgattgcttc gtcgcgatgg acagttcgtc gctcgcaagt gtctgatgtc
     2221 cgttcttgcg tgcgtacggc gcaatccctg ccagcccctt cgaatggtag gcctggtagc
     2281 gcgccagcaa cccctcgcgt atcagcgtct caaccttctc aatcggcacc gccccatcct
     2341 tgctggcgga cttcagcgct cgaaaggccg cgatctcctt cgcatccaga ttcaggtcat
     2401 cacccggttc cgcctcgagg tagcgccgag cttccttctc tccgttcggt gccagcttca
     2461 cccctgcgaa gtcggccagt gttccctcgc ccacgatctt gccatacgca atgatcgccg
     2521 agacattctt aaacgcagcc gcctcacgaa agagcgcaac aagattttca gtttttgtct
     2581 tggcccgcat ggccatgccg agggcaagtt ctcgatccga tccttcagtg gctgaccagg
     2641 tgacaatttt gccctcccgg atgctctgcc ggtcgctatc cgaaagctgc aactcctgca
     2701 acagttcctc ggcggtgggc agcgctgagg cctcaaggac aggactgcat aacaccaggc
     2761 cgacccacat gctcaggacg gctcgggtga cggccgggag gctgacgacc ccatagtgga
     2821 ggatagggga acaggacttg gtttgtgttc ttcttgattt catgggctcc tcttctgggt
     2881 ttcccctcat gggctcgcgc ttgttaccac agagctttct ttgtggaccg gcctcagaat
     2941 aagcttaatc gattgagaaa tcgacaaggc aagcgtgcat acgggaaaag gattttggca
     3001 ggagtgagct gagtggctcc cgaatgaaga ccgctagaga ttctaggtgg ttgaaaagtg
     3061 gtggagggca cgggagttga acccgcgacc ccgacgttgc gaacgtcgtg ctctcccaac
     3121 tgagctagcc ccccacctgt cgggaccggc gacacgagtc atgaatcccg cagggttcgt
     3181 tgcacatgaa cgtgcagact ggatgctgcg gtagaagaac ggggaggaag taggctcgcg
     3241 tccccctgcg gctgcattat acacaaccgc gaggcaggac tctacccgaa gttggtatcg
     3301 gacctgattc acgaaggact gatgatggct tgcccttctg gcttcgctgc gtcgatgaac
     3361 acccgtcgac cttcaacccg aagacgtcct tcggcaaaga gctggaccgc ccgcggatag
     3421 attttgtgtt cctggtcgag aatccgggct gcgagcatct ccggcgtatc gtgatcaagc
     3481 accggaaccg ccgcctgcag aatgatcgga ccctcatcga caccttccgt cacaaaatgc
     3541 accgtgcatc cggcgagctt gcagccccaa tcgatcgcct ttttctgcac atcgagccct
     3601 ggaaacgccg ggaggagtga cgggtggatg ttcattatcc gattggcata ggcgttgacc
     3661 aacactgccg tcacgatttt catatatccg gcgagcagga ccagctcgac ttcatgatgc
     3721 tgaagcacct tgagaagctc cccatcatag gcctcacgac tatcaggccg tccagcaaac
     3781 ggcttgggat ccacgaagag atcattgaga ctgtgcttcc gcgcccgttc gagtgccaac
     3841 gcatctttct tattgctgat cacagccgca atgtgtgcat caacctgctt ggcttcgacc
     3901 gcatcgatga ccgcctggag atttgaccct cggcctgatg cgagtacagc gacgcggagc
     3961 ggagttggcc gtttagtcga catactccac caatggtttt tcatcgttcg ccgagacgat
     4021 gcgtccgatc cgatgggctc ggtctcccaa cgccgtcgcg cgttggatga cggcatttgc
     4081 cgcatcggac ggcaccacga ggattaaccc aatgcccata ttgaagacgc ggtacatctc
     4141 gtctcgtgcg accgcaccaa gcttagcgat cacgtcgaag atgggtggga ccttccatgc
     4201 actgcgccgc acctgagccc gcacgccagg aggaaagact cgcggaagat tctcggtgat
     4261 gccgcctccg gtaatatggg cgatgccttt gatagggcat
//
LOCUS       DATDNW010000217         4006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_166963, whole genome shotgun sequence.
ACCESSION   DATDNW010000217 DATDNW010000000
VERSION     DATDNW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166963"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VI359_05440"
     CDS             complement(<1..282)
                     /locus_tag="VI359_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HEY6973728.1"
                     /translation="MFRIIKKSLKTGVVTGQYSGAEAPVAPVAQEAKDKAKPFKRSLT
                     IREVDTGSCNACEMEMNALANPVYDIERFGIHIAASPRHADALVVTGPVT"
     gene            complement(377..1969)
                     /locus_tag="VI359_05445"
     CDS             complement(377..1969)
                     /locus_tag="VI359_05445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY6973729.1"
                     /translation="MVDAIAGVKQLKEAFGNSIEELPLVHGTPRLRMKKEDVPVVAHY
                     LHTHPGLRGALSLLWAVDHRPHEARYELCYLFTLAERKDWLLLCTDIQSDDRLFASIT
                     PHIHAAKWYEREIRDMFGLIPVGHPDMRRLIRHEHWPKGSHPLKKDFRWDTVLAHAQG
                     QYAFRKIEGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPIMQLELHHFWKHRGIEKLFE
                     QQTLTTAVPLAERVSGDTTVGHSLAYCQAVETLLRIDVPRRARYLRSFFLELERLHNH
                     LGDIGAICNDTAYVLAHAHGSRLKERLMQLNDRLTGSRFLRGAMQVGGVAHDLDARLL
                     DETVDELQRVEEDFSELESIIFANASLTDRLETTGVLTERTAWDHAVMGLVGRASGID
                     RDARRDRPFAAYDELSVKTVLYRYGDVRARLRVRMDEIHESIRLIREIRLTIPNGAIA
                     AGPNRSANPGDWALSAVEGWRGEILYMVMAGEAGRIHRCKVRDPSFVNWPAIQWAVLG
                     NIIPDFPLINKSFNLSYAGNDL"
     gene            complement(1974..3518)
                     /locus_tag="VI359_05450"
     CDS             complement(1974..3518)
                     /locus_tag="VI359_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973730.1"
                     /translation="MQTIIAPVAVFLGAPLLAAFLSLATRHARVLHGLNLTTMTALVV
                     SECALIQQVLAHGPLIALWGLVYIDALSAFILFIVTVIGLSCSLYTWAYLDDYVARGV
                     IAAKRLNRFFFLFHLFLFAMIVATMANNLGVLWVAIEGTTLATTFLIAFFRKREGLEA
                     GWKYLILCSVGIALALFGTVLTYYSSVRVLGDESTALNVTKLLEVAGQLDPHVLKLAF
                     IFLFVGYGTKIGLVPMHTWVPEAYSEAPAPVTAMLAGVLETVAVYAVLRSKAVMDQAL
                     PPAFAGNLLLLFGLLSFMVAALFILIQHDYKRLFAYSSVEHMGLAMVGFGVGGPVGTF
                     GGLFHLLNHALAKSLAFFAAGNVHRRFATREIGEVRGLAKVQPVTAVAILIAGLALVG
                     MPPFSMFVSEVLVVSALATQSFASDTLHVGRFLTIIVADEVRSLSIVTVFLLFAVALF
                     GGFTYRIAAMVWGTPHESVKRGEGWDIGHVPLILTSLALVGLGMTLPDPLRTLLDRAV
                     SILVVR"
     gene            complement(3518..>4006)
                     /locus_tag="VI359_05455"
     CDS             complement(3518..>4006)
                     /locus_tag="VI359_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973731.1"
                     /translation="AWHLMNHAFAKSTAFYGAGLVFLRHNHKLLDRVTGLLNQMPVAG
                     TAILVAGVALAGMPPFGLFTSEVLIAVGAYTARPEVAYGFLALLGLAFATLLYQVLRM
                     ALGGPGEAGTALGRQCRLFTGAAVGINIAVLGIIGLRVPPGLETLLASIVKIFTVGME
                     AP"
BASE COUNT          919 a         1234 c         1102 g          751 t
ORIGIN      
        1 agtaaccggc cccgtcacca ccaacgcgtc cgcatggcgc ggcgaggcgg caatgtgaat
       61 gccgaaccgt tcgatgtcat agaccgggtt tgcgagcgcg ttcatttcca tctcgcaggc
      121 attgcaggag cccgtatcca cctcccgaat ggtcaacgac cgtttgaatg gtttggcctt
      181 gtccttcgct tcttgagcaa ccggcgcgac aggcgcctcc gctccggaat actggcccgt
      241 cacaactccg gtcttcaaac tctttttaat gatgcgaaac atggttcctc cttggctgtc
      301 agccttcgtc gatcagcttt cagctcagag ctgactgctg atggctgatg gctgattgct
      361 tcccgctctt ccgtcctcag agatcattcc ccgcatacga cagattgaaa ctcttgttga
      421 tcaaaggaaa gtcaggaatg atgttgccga gaaccgccca ctgaattgcg ggccagttga
      481 cgaatgaggg gtcgcgcacc ttacagcggt gaatccgacc agcttctcca gccatcacca
      541 tataaagaat ttcgccccgc cagccttcga ctgcagacag cgcccagtcc cctggattcg
      601 ccgagcgatt cggtccagcg gcgatcgcgc cgttggggat agtgagccga atctcgcgga
      661 tcaaacgtat ggattcgtgg atctcatcca tacgcacccg cagtcgggcc cggacatcac
      721 catatcggta cagaaccgtt tttaccgata actcatcgta cgccgcgaaa ggacgatcac
      781 gccgtgcatc acgatcgatc cccgatgccc gtccgacgag gcccatcacc gcatgatccc
      841 aggctgtgcg ttcggtcaat actccagtcg tttccaagcg gtcggtgagc gacgcgtttg
      901 caaaaatgat ggattccagc tcggagaagt cttcctcgac cctctgtaac tcatcgacgg
      961 tttcgtcgag tagcctagca tcaaggtcgt gtgcaacgcc gcctacctgc atggcaccac
     1021 gtaggaatcg tgagccggtc aaccggtcgt tgagctgcat gagccgctct ttcaaccgac
     1081 taccgtgggc atgggccagc acgtaggcgg tgtcgttgca gatggcgcct atgtcgccga
     1141 gatgattgtg cagccgttcc agttcaagaa aaaacgaacg caaatatctg gcacgcctcg
     1201 gcacgtcgat gcgcaggagc gtttcgaccg cctgacagta ggcgagactg tgtcccaccg
     1261 tggtatcacc cgacacccgt tccgcaagcg gcacggctgt ggtcagcgtc tgttgctcaa
     1321 agagcttttc aattccccga tgcttccaaa agtggtgcag ttccagctgc atgatgggct
     1381 ctccggcaac ggaaaaccgg aaatggcccg gttcgatgat gccggcgtgg attggcccaa
     1441 ccggaacctc gaagacccct tcgccttcaa tctttcggaa cgcgtactgc ccttgggcat
     1501 gtgccaagac agtgtcccat cgaaagtctt ttttgagtgg gtgggagcct ttgggccagt
     1561 gttcgtgccg gatgagccga cgcatgtccg gatggccgac agggatcaac ccgaacatat
     1621 cgcggatttc ccgttcgtac cacttggctg catggatgtg gggagtaatt gatgcgaaca
     1681 gacgatcatc actctggatg tccgtacaca gcagcaacca gtctttacgt tctgccagcg
     1741 taaacaggta gcagagttcg tagcgcgcct cgtgcggccg atggtccacg gcccagagaa
     1801 gtgacaaggc cccacgcagc ccgggatggg tgtggagata atgcgcgacc accggcacat
     1861 cctccttctt catccgtagt cgcggcgtgc cgtggacaag aggcagttcc tcaatggagt
     1921 tcccaaacgc ctccttcaat tgtttcactc ctgctatcgc atctaccatc gcatcacctc
     1981 acaaccagaa tactgaccgc tcgatccaac agcgttctga gtggatcggg gagcgtcatc
     2041 ccaagcccca ctaacgcgag actggtgagg atcaggggca catgcccgat atcccatcct
     2101 tcaccccgtt tcacgctttc gtgaggcgtt ccccacacca tggcagcgat ccgataggta
     2161 aacccgccga agagcgcaac cgcaaacaga agaaagaccg tcacgatcga caagctgcgc
     2221 acctcatcgg cgacgatgat cgtgaggaaa cgacccacgt gaagcgtatc tgaagcaaaa
     2281 ctttgggtcg ccagcgccga cacgaccagt acctcgctca cgaacattga gaacggcggc
     2341 atgccgacca gcgccaggcc tgcgatcagg atcgccactg ctgtgaccgg ctggactttc
     2401 gccagccccc gcacctcacc gatctcgcgg gtggcgaacc ggcgatggac attccctgcc
     2461 gcaaaaaacg ccagcgattt tgccaacgca tggttgagca gatggaacag gcctccgaat
     2521 gttccaaccg gtcctccgac cccgaacccc accatcgcca accccatgtg ttccacactg
     2581 gagtaggcga acaatcgttt gtagtcgtgc tggatcagga tgaacagcgc ggccaccatg
     2641 aaggaaagca gcccgaagag gagcaggagg ttcccggcaa aggccggggg gagtgcctga
     2701 tccatgactg ccttgctgcg cagcaccgca tagactgcga cggtctctaa gacaccggcc
     2761 aacatcgctg tgaccggtgc tggtgcctcg ctataggcct ccggcaccca ggtatgcatg
     2821 ggcaccagcc cgatcttggt tccatagccg acgaagagaa aaatgaacgc gagcttgagc
     2881 acatgcggat ccagctggcc ggccacttca aggagtttgg tcacatttaa ggccgtgctc
     2941 tcatctccca ggacccggac cgatgaataa taggtcaata ccgtgccgaa caacgccaag
     3001 gcgataccga ccgaacagag aatcaggtat ttccatcctg cttccagtcc ttcacgtttg
     3061 cggaagaatg cgatcaggaa cgttgtggca agcgtcgttc cttcaatggc cacccacaga
     3121 acgcccaggt tattcgccat tgttgccacg atcatggcaa acagaaagag atggaacagg
     3181 aagaaaaacc gattcagccg cttcgccgcg atcacacccc gcgccacgta atcatcgaga
     3241 taggcccacg tgtacaacga gcaggacaag ccgatgaccg tcacgatgaa caggatgaag
     3301 gcggagagcg cgtcaatata gaccaacccc cagagcgcga tcaggggtcc gtgagccagc
     3361 acctgctgga tgagggcaca ctcggacaca accaacgcgg tcatcgtggt gagattcaac
     3421 ccatgcaaca cgcgcgcgtg cctggttgcc aaactgagaa aggccgcgag cagcggtgcg
     3481 ccgagaaaaa ccgccaccgg ggcgatgatg gtctgcatca cggcgcctcc atcccgacgg
     3541 tgaagatctt cacgatcgac gccagcagcg tctccagccc cggtggaacg cggaggccga
     3601 taattcccag cacggcaatg ttgattccga cagcggcgcc tgtaaaaaga cggcactgtc
     3661 tgcccaacgc agtacctgcc tcacccggcc ctccgagcgc catccgcaat acctgataga
     3721 gcaaggtcgc aaaggccagc ccaagcagag ccaaaaaccc ataggccact tcgggcctgg
     3781 cggtataggc tccaacggca atgagcacct cgctggtaaa gagtccgaag ggcggcatgc
     3841 ccgccaacgc gacaccggcc acaaggatgg cggttcctgc gaccggcatt tgattcaaca
     3901 gcccagtcac gcgatcgagc agtttgtggt tgtgccggag gaagaccaga ccggcgccat
     3961 agaaggccgt ggatttggcg aacgcgtggt tcatcagatg ccacgc
//
LOCUS       DATDNW010000218         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_646063, whole genome shotgun sequence.
ACCESSION   DATDNW010000218 DATDNW010000000
VERSION     DATDNW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646063"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="VI359_05460"
     CDS             complement(<1..1504)
                     /locus_tag="VI359_05460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY6973732.1"
                     /translation="MQSAGFPWLSLLILLPLAGAAALLFVKDLAVRWLALGVTIADLV
                     IALPLWWLFDPSSGQMQFSEHVVWITSPPIHYSLGLDGISLPLVLMTAGLLPLCVLAS
                     WQAVATRIRGFMAMLLLVEAAMLGVFMALDFVLFYVFWEAMLIPMYLLIGIWGGPNRL
                     YAAIKFFLYTLAGSVLLLVAILVLFFYGGHTFDIMALTQVSYSTPLQMWLFLALFAAF
                     AVKVPMFPFHTWLPDAHVEAPTAGSVLLASLLLKMGTYGFIRFSLPILPDASRAFTPM
                     MVTLSIIAIIYGAYMALAQADLKKLIAYSSVSHMGFVTLGLFVSNQQGIEGAVLQMVN
                     HGITTGALFLCVGVIYERTHSRLIADNVGLTKPMPQYATFLVIFALSSLGLPGTNSFV
                     GEFLVLAGTFLWSKVATALASLGLILAATYLLWMVQRIAFGVPSPRHLPELRDLNQRE
                     LAMMVPLAVLVFWIGFFPNPLVSRMHTSVMKTIDLMSRTRVAPATHPSAGH"
     gene            complement(1511..>2386)
                     /locus_tag="VI359_05465"
     CDS             complement(1511..>2386)
                     /locus_tag="VI359_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973733.1"
                     /translation="HLLTHGAFKALLFLGCGSVIIALHHEQDMRQMGGLKDKLPVTYW
                     TFVVGSLALAGFPLTAGFFSKDDLLVSTWFAGPLGQILTIFGLLTALMTAFYSFRLVF
                     VTFWGPSHVDPHHAGHVHEPSKTMTVPLIVLAVLSIVTGYLGIPAFLEPVFATGSDTS
                     PHHGGGAAGIMMLATVMGLFGIAGAYYLYVARPGLPDQLARMWQALYQGSLHKWYVDE
                     AYDRTVVRPTFSMADELWTRIDVRIIDGAVNGVARAIAWSGWLLRLLQSGQTQHYALG
                     MALGAVVFLTVLLIF"
BASE COUNT          578 a          667 c          751 g          390 t
ORIGIN      
        1 catgtccggc cgagggatgc gttgcgggag cgacgcgtgt ccgtgacatc aggtcgatcg
       61 tcttcatgac gctcgtatgc atccggctga cgaggggatt ggggaagaag ccgatccaga
      121 agaccagtac cgccagcggc accatcatgg ccaattcgcg ttgattcaaa tctctgagct
      181 ccggtagatg gcggggagaa ggaacgccga aggcgatccg ttggaccatc cagagcaggt
      241 aggtcgccgc gagaatcagc ccgagcgacg ccagtgccgt ggcgaccttg ctccagagaa
      301 atgtccctgc cagcacgagg aactctccga caaaactatt ggtgccgggc aagccgagcg
      361 aggagagagc gaagatgacc aaaaaggtgg cgtattgcgg catgggtttg gtgagcccca
      421 cgttgtcggc gatcaagcga ctatgggtgc gttcatagat cacgccgacg cacaagaaca
      481 acgctcccgt cgtaatgccg tggttcacca tctggagcac cgcaccttcg atgccctgct
      541 gattcgagac gaagaggccg agcgtgacga atcccatgtg actgacactg gagtaggcga
      601 ttagtttctt gagatcggcc tgggccaacg ccatgtaggc tccgtagatg atggcgatga
      661 tcgacagcgt aaccatcatg ggcgtgaacg ctcggcttgc atccggcaat atcggcaggc
      721 tgaaacggat gaatccatag gtgcccatct tcaacaacag gctggcgagc aacacactcc
      781 cggcagttgg cgcttcgaca tgcgcgtcgg gaagccatgt gtggaaaggg aacatcggaa
      841 ccttcacggc gaaggcggcg aacagtgcca agaagagcca catctggagc ggcgtcgagt
      901 acgacacttg tgtcaaggcc atgatgtcga aggtgtggcc gccatagaaa aacagtacga
      961 ggatcgcgac aagcagcaga acgctaccgg ccagcgtata caggaagaac ttgatggcgg
     1021 catacagtcg gtttggccct ccccatatcc cgatcaggag gtacatcggg atcagcatcg
     1081 cctcccagaa cacatagaaa agcacgaagt ccaaggccat gaacacgccg agcatcgccg
     1141 cttccaccag gagcagcatg gccatgaagc ctcggattct cgtcgcgacg gcctgccacg
     1201 acgcgagcac gcagagcggc aggagccccg ccgtcatcag gaccaatgga aggctgattc
     1261 cgtcgaggcc caggctgtag tggatcggag gagaggtaat ccagaccaca tgttcactga
     1321 attgcatctg gcctgaactc gggtcgaaga gccaccagag cggcagggca atcaccagat
     1381 cggcgatcgt cacgccgagg gcgagccagc ggacagccaa gtctttgaca aacaagagcg
     1441 ccgcagctcc cgccaagggg agcaggatca gcagcgagag ccatggaaat ccggcggatt
     1501 gcatcggacc tcagaagatc agcaagacgg tcaagaacac cacggcgccg agcgccatgc
     1561 cgagcgcgta atgttgtgtt tgtccactct gcaggagacg cagaagccag ccgctccatg
     1621 caattgcccg ggccacaccg ttgacggccc cgtcgatgat cctgacgtca atccttgtcc
     1681 agagttcgtc agccatggaa aaagtggggc gcacaacagt gcggtcatag gcttcatcga
     1741 cgtaccactt gtggagtgag ccctgataca gagcctgcca cattcgggcc aattgatccg
     1801 ggagacctgg cctcgccacg tagaggtagt acgctccggc aatcccaaac agtcccatga
     1861 cggtcgccag catcatgatg ccggcggctc caccgccgtg gtggggagat gtgtccgagc
     1921 cggtggcgaa gaccggttcg agaaacgccg gaatgccgag gtagccggtg acgatgctga
     1981 ggacagccaa gacaatgagc gggacagtca tggtcttcga gggttcgtgc acgtggccgg
     2041 catggtgagg atccacgtgc gatggccccc aaaatgtgac gaagacgagt cgaaaactgt
     2101 agaacgcggt catcagcgcg gttagcaagc cgaagatggt caagatctga ccaagcgggc
     2161 cggcgaacca ggtcgatacg aggagatcgt ctttgctgaa aaagccggcg gtcagtggaa
     2221 atccagccag cgcgagcgac ccgacgacga aggtccagta ggtcacaggc agtttgtcct
     2281 tgaggcctcc catctgccgc atgtcctgct catgatggag cgcgatgatc acggagccgc
     2341 agcctaagaa cagcagggct ttgaaggctc cgtgggtcag gaggtg
//
LOCUS       DATDNW010000219         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_502388, whole genome shotgun sequence.
ACCESSION   DATDNW010000219 DATDNW010000000
VERSION     DATDNW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502388"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="hisZ"
                     /locus_tag="VI359_05470"
     CDS             <1..1213
                     /gene="hisZ"
                     /locus_tag="VI359_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY6973734.1"
                     /translation="ILPTLEYLDVLAPGLEAELVEKSYKLADRTTGRMLLLRPDATAQ
                     IARTVGMGLTGSTLPLRLSYRTSVFRYQPEHGGRDREIFQVGAELIGVDDGGGDSEII
                     SLLIECLRAIGLRSFTVSVGHVGFSKGLLVRAGLSAQGQKHAEQAVAKKDLPRLEEVL
                     AREGVSKTLTAAILEAPELYGREEVLERGRALAAGDPMLQGPLDRLAQVYQLLCVSGY
                     GESLLLDLGEFRGFDYYDGVVFDVFAEGVGAELGGGGRYDHLIGRFGRPLPSTGFALD
                     VDRIFHAAVNGNGDEEWLKAEYLVAASRRGARRVMSVASQLRQSKSRVIQCLVKGADD
                     KVLTDAIAMGEAQRAGAVVVVGAPGTAQDEVLLVDLPSKKVGRPRAKTVKMKDLVALA
                     RRNRRSGKEQA"
     gene            1210..2565
                     /gene="dnaB"
                     /locus_tag="VI359_05475"
     CDS             1210..2565
                     /gene="dnaB"
                     /locus_tag="VI359_05475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY6973735.1"
                     /translation="MKSMSDVDLSQPRMPPQNIEAEQSILGAILLDKEAMPKVEELLV
                     DQDFYRTAHQKIYRAMLNLSERDEAIDHITLSEHLRSLGELEGVGGAAYLAELVAVTP
                     SAANVRYHCKVVKDKALLRGLISASTDVLMQAYDSTESAEVVLESAEQSVFRIGQNRL
                     GRGFYPLKEVIAESLGVVDALHKLGKSVTGVPTGFAELDEITAGLQPSDLVILAARPS
                     MGKTSLALGIAIKAAIDHQQKVGIFSLEMSKEQLVLRMLSSHASVDSHKLRIGKLDKE
                     EWWALAEAAGRLEMAPIFIDDSGSLSVTQMRGKARRLMKEKGLDLLVVDYLQLMQGRP
                     DAESRQQEISDISRSLKALAKELQVPILALSQLSRAVENRTDKRPVLADLRESGAIEQ
                     DADVVIFIYRDEVYNSDSDEKGTAHILVKKHRNGPTGDLKLTFLDKFAKFADFSDREE
                     T"
BASE COUNT          637 a          628 c          802 g          648 t
ORIGIN      
        1 tattctgccg acgcttgaat atctcgatgt tttggctcct ggtcttgagg cagagctcgt
       61 tgaaaaaagt tacaagctcg ccgaccgcac gacgggacgg atgctgttgc tcaggcccga
      121 tgcaactgct caaatcgccc gcacagtcgg gatgggattg acgggatcga cgcttccttt
      181 acggttgtcc taccggacgt ctgtattccg gtaccagccg gaacatgggg gacgggaccg
      241 ggaaattttc caagtaggcg ccgaactcat cggggtcgat gacgggggcg gtgacagcga
      301 aatcatcagc ctgctcattg aatgcctgcg tgccatcggc ctccgctcct tcactgtgtc
      361 cgttggccac gtgggatttt ctaaaggtct gttggtgcga gccggtctct cggcgcaagg
      421 ccagaagcat gcggaacagg cggtggccaa aaaggattta cctaggctgg aggaagtgtt
      481 ggcacgtgaa ggcgtttcga aaactctgac cgcggcgatt ctcgaagcgc cggagctcta
      541 cggccgtgag gaagtacttg agcgcggtcg ggctctcgcc gcgggagatc ctatgttgca
      601 gggtccgctt gaccggctcg ctcaggtgta tcagctcctc tgtgtttcgg gttatggcga
      661 atcgttactg ttggatctag gagaatttcg aggattcgac tactatgatg gcgtagtgtt
      721 cgacgtattt gctgagggag ttggagccga acttggtggt ggagggcgct acgatcactt
      781 gatcggtcga ttcgggcgtc cgttgccttc gacggggttc gccttggatg tcgatcggat
      841 ttttcatgcg gcggtgaatg gcaatggaga tgaagaatgg ctcaaggccg agtatttggt
      901 ggcggcgtct cgccgagggg ctaggcgcgt gatgtcggtc gcaagtcagt tgcggcaatc
      961 taaatcacga gtgatccaat gtctggtcaa aggagccgac gacaaagtat tgactgatgc
     1021 gatagccatg ggagaggccc agcgagccgg agcggtggtc gtcgttggtg ctcccggcac
     1081 tgcacaggat gaggtgctgc tcgtggatct gccaagcaag aaggttggcc gacctcgcgc
     1141 gaaaactgtg aagatgaaag atctagttgc ccttgctcgc cgaaatcgtc gaagtggtaa
     1201 ggagcaagca tgaagtccat gtcggacgtt gacctatctc aaccaagaat gcctccgcaa
     1261 aatattgaag ctgagcagtc aatcttgggc gctatcctac tcgataaaga ggcgatgcca
     1321 aaggttgagg agctgctggt agatcaggat ttttatcgta cggcgcatca gaagatctat
     1381 cgagccatgt taaatctttc tgagcgagat gaagcaatcg accacattac gctgagcgaa
     1441 catctaagaa gcttgggaga actcgaagga gttggcggtg cggcctacct tgcggagctt
     1501 gttgctgtca cgccatctgc ggccaatgtc cgctaccact gtaaagttgt gaaagacaaa
     1561 gctctcttac ggggcctcat cagtgcctca acggatgtgt tgatgcaggc atatgattct
     1621 acggagtctg cagaggtggt gcttgaatct gctgaacaat cggtctttag gataggtcaa
     1681 aacaggctgg gacgaggatt ctaccctctg aaagaggtta tcgccgagag tcttggggta
     1741 gtagatgctc tccataagtt aggaaaaagt gtgacaggcg ttcctactgg atttgcagag
     1801 ctggacgaga ttactgcggg actccaacca tctgatctcg ttatcttggc agctagaccc
     1861 agtatgggca aaacaagtct tgccttggga attgcgataa aggccgcaat tgatcatcaa
     1921 cagaaagtgg gaatatttag tttagaaatg tccaaggagc agcttgtttt gaggatgctt
     1981 agctcccatg cgtcagttga ctcgcacaag cttaggattg gcaaattaga taaggaggag
     2041 tggtgggcgc ttgctgaggc agcaggtagg cttgagatgg cacccatttt catcgatgac
     2101 tcgggtagcc tttcggtgac acagatgcgt gggaaggcga gacgcttgat gaaggagaag
     2161 ggactagacc tgcttgtcgt ggactacctt caactcatgc aaggtaggcc ggatgccgaa
     2221 tcgagacagc aagagatctc tgatatttct cgttccttaa aagcgctagc aaaggaattg
     2281 caagttccga ttcttgcctt atcccagttg agtcgagctg tcgagaacag aactgacaag
     2341 aggcctgtac ttgccgacct aagggagtcc ggcgcgattg aacaggacgc ggatgttgtc
     2401 atcttcattt atagagacga agtatacaat tccgattccg atgaaaaggg gactgcacac
     2461 atactggtaa agaaacaccg taatggcccc acgggagatt tgaagcttac cttccttgac
     2521 aagtttgcca agtttgccga tttctcagac cgagaagaaa cctgaactcg tttgaccccg
     2581 cttcactccc cctcgtataa tctccttgag ttctacccga gacgatttct gagatgatca
     2641 tgcgaatctt gatgagcgat cggatttcta caccctcgat cgtcgccaga tataccacat
     2701 cgggcttcgg cattt
//
LOCUS       DATDNW010000220         3067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_790840, whole genome shotgun sequence.
ACCESSION   DATDNW010000220 DATDNW010000000
VERSION     DATDNW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790840"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..606)
                     /locus_tag="VI359_05480"
     CDS             complement(238..606)
                     /locus_tag="VI359_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973736.1"
                     /translation="MKLVIGTVATLSGVLFLLSMASANPALLPKHEGYPMKNSGSPVN
                     GQATANDPGQTNASGEKAELKAAAFDDSHVKQNLIKTDNERITKNQGANQLPTVQGPQ
                     ITIAPPVTSATKADASLGVK"
     gene            complement(772..2589)
                     /locus_tag="VI359_05485"
     CDS             complement(772..2589)
                     /locus_tag="VI359_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973737.1"
                     /translation="MTTAAPHSKPNAETHLQRTLTGVLNELPVDAALAAIFHREQGPL
                     SGHAARGFSPRDIQAILRTLSAPAIAGTSAASDQDGSRTMRLQLITPAAKSLLGAPLR
                     HRNRTYGYLVIGRKESASFTKKEKTLMDQASEDVTKALDREGLFDLNMVLSRPYVANE
                     PMPATASGVEIFAAPASQLTPEVQEKIVAILLDASQFVAYDRAWAGHYDPIAGGVEVL
                     GIVGDQKGDTKDGKKDLKVGQRLVLDGSAAGWAVRHRKPRVDHDLASTQGRFLDHKHL
                     FKDRFQTSLVVPFFVRGQVGGTITLSSKEAGRYQPTDARTLEPIILKLADALQAPAPT
                     TPGPTPALGSVDTPEGAVTPTSLEPIIRKQERHEAIGEFSAFLATEVREPLGSIRAQL
                     EELTGEGILDFDPQTRVENAMRELMRVEAILNEILDFAKPLELNRRLCRIPEVLESAL
                     TVVGTDLEVTRIQVTKDYATQIAPVRGDESKLQQVFLSIFKNAVEAMSPGGHLHIQVT
                     QHRAGRGLEDQILIKNDGAPIPAEIVDKVFEPFFTTKRAGTGLGLATVKKIIEEHGGS
                     IAIGSAPGEGTTVTIRLPGISRGPAFRHRGRGRRPLHRR"
     gene            complement(2747..>3067)
                     /locus_tag="VI359_05490"
     CDS             complement(2747..>3067)
                     /locus_tag="VI359_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY6973738.1"
                     /translation="GLAAVFVLTLLNLLVRPILFVLTLPLIVLSLGLFLVVLNALLLE
                     LTAYLVKGFSVDGFWPAVGGAIIISLVTTVLNAWTVEHQRIEAQPFPQRPPTIINPDE
                     KREP"
BASE COUNT          621 a          818 c          955 g          673 t
ORIGIN      
        1 tgggaaccag ttgtggttcg aagatcggtc tgttgcggta cggaagtcgg atgtcggtgc
       61 attcacacgc attggcgtcg attatgcggg gaaatgggcc aagaagctct ggcggtttcg
      121 gctgaatgga gtgaagagcg agagccggaa gcggagctga tacatgccgt caaaacatga
      181 agagggagcc aggctgcatg cctgactccc tcttcgcaga agaagctacg attcgagtta
      241 ctttacgccg aggctcgcgt cagccttagt cgcggaggtc accggaggag caatcgtgat
      301 ctgtggcccc tgcacggtcg ggagttgatt cgcgccctgg tttttggtaa tccgctcatt
      361 gtcggtctta atgagattct gcttgacgtg cgagtcgtca aacgcggcgg ccttgagttc
      421 ggccttctca ccacttgcat tggtctgacc cgggtcattg gcagtcgctt gcccgttcac
      481 ggggcttccg ctgttcttca tgggatagcc ttcgtgcttg gggagcagcg ctggattggc
      541 agacgccatc gacagcagga acagcacacc ggacagtgtc gcaacagtgc cgattacgag
      601 cttcataccc tactcctttg tgaataaagg tggctgaagg aatacgttga tgaatacgtt
      661 cattctaaag gccaatcaag tggcgggatg atagccaggg ggggctatct ctgtcaagcg
      721 agaaaggagc tgcgcggtaa acgacagaaa aagcagggtt tgtggaaaat cctagcgccg
      781 atggagggga cggcgaccac gaccacggtg gcggaaagcg gggccacggc tgataccggg
      841 aaggcggatc gtgactgtcg tgccttcgcc aggcgcactg ccgatggcga tcgaacctcc
      901 atgctcttcg atgattttct tcacggtcgc gagaccgaga ccggtgcctg ctcgtttcgt
      961 cgtaaagaac ggctcgaaca ccttatcgac gatctccgcc gggatcggtg ctccatcgtt
     1021 cttgatcaga atttggtcct cgagacctcg accggctcga tgttgggtca cttgaatgtg
     1081 caagtgcccc cccgggctca tggcttcgac tgcgttcttg aagatgctca agaaaacctg
     1141 ctgaagtttg gattcatcac cgcgcactgg ggcgatttgc gttgcatagt cttttgtcac
     1201 ttgaatccgc gtgacttcta aatcggtccc gacgacagtc aacgcgctct cgagaacctc
     1261 ggggattcga cagagacggc gattcagttc gagcggtttc gcgaagtcca gaatttcatt
     1321 caggatcgct tccactctca tgagttcgcg catggcgttc tcgacgcggg tctggggatc
     1381 aaaatcgaga atgccttctc ccgtcagttc ctccagttgc gcgcggatgg atcccagcgg
     1441 ttctcgaact tccgttgcga gaaacgcact gaattcaccg atcgcttcat ggcgttcttg
     1501 cttgcggatt atgggctcca acgaggtagg tgtcacggcg ccttcgggag tgtctaccga
     1561 tcccagagcc ggtgtcgggc cgggtgtcgt aggagccgga gcctgcaaag cgtcggcgag
     1621 cttgagaatg atcggttcga gcgtgcgcgc gtccgttggt tggtaccgac cggcttcttt
     1681 tgacgagagc gtgatcgtac cgcccacttg gccacggacg aagaacggta cgacgagcga
     1741 ggtctgaaac cgatctttga agaggtgttt atggtcgaga aaccgtcctt gggtcgaggc
     1801 cagatcgtga tcgacacgag gcttgcggtg gcgcaccgcc catccggcag ccgagccatc
     1861 taacaccagc cgctgaccga ccttcagatc ttttttcccg tctttggtat cccccttttg
     1921 atctccgacg ataccgagta cttcaacgcc tccggcgatc gggtcgtagt ggccggccca
     1981 ggcccggtcg taggcgacaa attggcttgc atccaggagg atcgccacga tcttttcctg
     2041 cacctccggc gtgagctggg aagccggtgc ggcaaagatt tcaacaccgg aggccgtcgc
     2101 aggcatcggt tcattcgcga cgtacggccg gctcagcacc atgttcaggt cgaacaggcc
     2161 ttcacgatcc agcgcttttg tcacatcctc gctagcctgg tccatcaagg ttttttcttt
     2221 tttcgtaaag ctcgcgcttt ctttgcggcc tatgacgaga tacccgtatg tgcgattgcg
     2281 atggcgcagc ggcgcaccca acaaagactt ggcggcggga gtaatgagct gcaggcgcat
     2341 cgtccggctc ccgtcctggt cgctggcggc actggtgcct gcgatcgccg gagccgagag
     2401 ggtacgaagg atcgcttgga tatcgcgtgg tgaaaacccg cgtgccgcat gacctgagag
     2461 ggggccctgt tcacgatgaa agatggccgc cagggcggcg tcgaccggca actcattcaa
     2521 gaccccggtg agagtccgtt ggaggtgcgt ctcggcgttg ggtttgctat gcggtgccgc
     2581 ggtggtcatg gctgagcgtg cttcacaatg gtcgcattgt agcaatgggg gcggggcaat
     2641 tcaatgaaaa gggatgagcg ctgattgaag cgcgggaagc gagacgaaac ggggttcatg
     2701 ttgcaacgaa gctgtgttga ggtgccgcat gcttatgtct cacgtgtcag ggttcacgtt
     2761 tttcgtctgg attgatgatt gtcggtgggc gttggggaaa cggttgagcc tcgatccgct
     2821 ggtgctcgac cgtccaggcg ttaaggacgg tcgtgaccag actgataata atcgcaccac
     2881 cgacggctgg ccagaacccg tccaccgaaa atcccttgac caagtacgcg gtgagttcca
     2941 ggaggagcgc attaagcacc acgagaaata atcccagcga cagcacgatc aacgggagtg
     3001 tcagtacgaa caggatcgga cgcaccaaca gattcagcag tgtgaggacg aagacagccg
     3061 caaggcc
//
LOCUS       DATDNW010000221         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_986510, whole genome shotgun sequence.
ACCESSION   DATDNW010000221 DATDNW010000000
VERSION     DATDNW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986510"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VI359_05495"
     CDS             <1..675
                     /locus_tag="VI359_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEY6973739.1"
                     /translation="RRLRQPYVEHDDSSPCGTPFLGNQTGLIISKEITRVLFDIMPAM
                     SGPWQIMLVASCLTTGACATTYDASQDTTGGTPQITFVQLKAAPDSFKGQSVVFGGEV
                     LTAKRLKEGTRIEILQLPLDQSSRPGMNLAQSQGRFLAIHRDFLDPATLPYGTRVTVT
                     GDVTGSVTMPLDEVDYIYPVIDIKTIRVWSDIDDVAPRIRPYYYGPWPYWGPYWGPYG
                     RPWPYW"
     gene            complement(757..1836)
                     /locus_tag="VI359_05500"
     CDS             complement(757..1836)
                     /locus_tag="VI359_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973740.1"
                     /translation="MGGTTEHRSVRHVILVFSVLFLSFFPISISFPASPIQQDPAQRQ
                     IERGLLLLHQGELKGATVAAKKAIELIASSAEAYHLLGLIYFKERKPAEAAEAFSRAV
                     KLKPAYPEALNDLADVYLAQGKAAEAERTLTRAIEVDPQHVDSYLDLAKLYEQRHNVV
                     AATKTYQSLLSVQPNQTKALFSLASLYDTQGDSKSAKDTLSRLVKVDPTHADAWYLNG
                     RIAERRNDLVEAAYSYKQAIVAKPDFVDAHYNLAFIHQSQGQPREAEREFLEVIRYRP
                     EYAEAHMNLGVIYTSLNRLDEAEQAYERAVALKPNYAEGHYNLGVFYELHRKDLPRAL
                     AQYHKYRDLGGRDDRVERIIGSGGR"
     gene            complement(2032..>2248)
                     /locus_tag="VI359_05505"
     CDS             complement(2032..>2248)
                     /locus_tag="VI359_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973741.1"
                     /translation="KTYGHTFTVLMDRDNKVANQFGVFGLPVSVFIDQQGRVQEYIKG
                     GLLTEHIINDVVARIQKQEPVKAASLR"
BASE COUNT          502 a          659 c          577 g          510 t
ORIGIN      
        1 agacgattgc ggcagccata cgtcgaacac gatgactctt ccccatgcgg cactcctttc
       61 ctcggtaatc aaaccggtct cattatatcg aaagaaatca cgcgcgtgct gtttgatata
      121 atgccagcca tgtccggacc atggcagatc atgctcgtgg cctcctgcct cacaaccggc
      181 gcatgtgcca ccacatacga cgcaagccag gacaccacag gaggcacgcc tcagatcacc
      241 tttgtgcaac tcaaggcagc gccggattcc ttcaaaggcc aatccgtcgt ctttggaggc
      301 gaagtcctca cggccaagag attgaaagag ggaaccagga tcgaaattct ccaactgccg
      361 ctcgatcaat cgtcccgtcc cggaatgaac ctggctcaat cacaaggacg ttttttagcc
      421 atccaccgtg actttctcga tcccgcgacg ctgccttacg gcaccagagt caccgtgaca
      481 ggcgacgtga ccggatcggt caccatgccg ttggatgaag tcgactatat ttatccggtg
      541 attgacatca agacgatacg ggtatggtcg gatattgacg acgtcgcccc tcgtatacgg
      601 ccctactatt acggtccttg gccttattgg ggaccctatt ggggccccta cgggcgcccc
      661 tggccctact ggtgaaatgc actcccacta tcagagcatg agaaaagatg aagcctcgtg
      721 ttctttaatc acggaacacg gggccgtcct atccaattat cgtcctcctg aaccgataat
      781 ccgctccact cgatcgtctc gcccacccaa gtcgcgatat ttatggtatt gcgccaacgc
      841 acggggaaga tctttgcggt gtaattcgta aaacactcca agattgtaat gcccttccgc
      901 ataattcggc ttgagtgcca cggcccgttc ataggcctgc tccgcctcat ccaatcgatt
      961 gaggctcgta taaatcacac cgaggttcat atgcgcttca gcatattcgg gacgataccg
     1021 gatcacttcc agaaattccc gttcagcctc ccgcggctgg ccctggcttt gatggataaa
     1081 ggcaaggttg taatgtgcgt ccacgaaatc cggtttcgct acaatcgctt gtttgtagga
     1141 ataggcggcc tcgaccagat cattgcgcct ctcggcgatc cgtccgttca agtaccacgc
     1201 atcggcatgg gtcggatcaa ctttcaccaa ccggcttaac gtgtctttcg cggacttgct
     1261 gtcgccttgc gtgtcgtaga ggctggccag gctgaacagc gctttggtct gattcggctg
     1321 tacggacaga agcgattgat aggttttcgt cgcagcgacc acgttatgcc gttgctcgta
     1381 caacttggcc aggtcgaggt aggaatccac atgctgggga tcaacttcga tcgcgcgggt
     1441 cagcgttcgc tccgcctcgg ccgcttttcc ctgtgccagg tacacatcgg cgagatcgtt
     1501 caacgcttcg ggatacgccg gcttcagttt caccgcgcgc gaaaaagcct cggccgcttc
     1561 agccggtttt cgctctttga agtaaatgag gcccagcagg tgatacgcct cagcggacga
     1621 ggcgatcaac tcgatggcct tcttggccgc tacggtcgcg cctttcaact ctccctggtg
     1681 caacagcaat agaccccgct cgatctgccg ttgagccgga tcctgttgga tgggagacgc
     1741 ggggaaagaa atagagatgg gaaagaacga aagaaacagg acggaaaaga ccagaatgac
     1801 atgcctgaca gatcggtgtt ccgtcgttcc ccccatactt accctcacaa tgaaaccgcc
     1861 cgaccggact ataaacggca ggagacggcg aaatcaataa tgagcgccta ccgaactgca
     1921 ttggaggaca tcatccgacg gtgatcgcta atcgcatgcg cgaacgaatc gatggcgaat
     1981 tcgattactc ctctccgttc agaattgtcg aatcacactg gccatcattt gctatcggag
     2041 cgaggctgct ttcaccggct cttgcttctg aattctcgcg acgacgtcgt tgatgatgtg
     2101 ttcagtcagc agccccccct tgatgtattc ctggacacgc ccctgttgat cgatgaacac
     2161 gctcacgggt aggccgaaca cgccgaactg gttggccacc ttgttgtccc gatccatcag
     2221 gacggtgaac gtatggccat aggtcttg
//
LOCUS       DATDNW010000222         5504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_369221, whole genome shotgun sequence.
ACCESSION   DATDNW010000222 DATDNW010000000
VERSION     DATDNW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369221"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /gene="ureB"
                     /locus_tag="VI359_05510"
     CDS             complement(<1..345)
                     /gene="ureB"
                     /locus_tag="VI359_05510"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY6973742.1"
                     /translation="MAKKTKRSPAKVHKASQKKGSSLAAMIKAELFRPVVPGQILFGA
                     GDIDANAGRATKELLVANTGDRPIQVGSHCHFFEANRALKFDRALAFGFRLCIPAGTA
                     VRFEPGEEKRVTI"
     gene            complement(393..695)
                     /gene="ureA"
                     /locus_tag="VI359_05515"
     CDS             complement(393..695)
                     /gene="ureA"
                     /locus_tag="VI359_05515"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY6973743.1"
                     /translation="MHLTPREQEKLLIYTAGQLAADRKKRGLKLNHPEAVAYLTAAVL
                     EGIRDGKSVAELMTYGTTLLSRKDVMPGVAEMIHEFQVEGTFPDGTKLVTVHDPIR"
     gene            865..1914
                     /locus_tag="VI359_05520"
     CDS             865..1914
                     /locus_tag="VI359_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEY6973744.1"
                     /translation="MAHVALKDRFRDHPTFGFVDWVLRGIGQVVFQNNPISGAIILGA
                     IFYNSWIYGAVCLFGTIISTLTALLFRADKGMIKDGLFGFNGALIAIALVAYTSQNFT
                     TGDMPNLHLWLYIVLSATFTSVLMPAFGAVLGPHKVPGLTMPFVLATWFFLGALLQFS
                     TIDVSNALKATSPTDFTGPRPDYTWITWFHGITMGISEIFFQDNWVTGVIILIGIAIN
                     TRIGALMALMGSTLAVAAAVLYGAHDEAIRDGLFGYNAALTALALGGLFYVLNLPGFI
                     YTVIGVLVTARVWASLGVFLEPSGMPVLTSAFVFVTWLMLLAKNGFPSLIPVAPAEAT
                     TPEENLERYRANQKR"
     gene            complement(1902..3215)
                     /locus_tag="VI359_05525"
     CDS             complement(1902..3215)
                     /locus_tag="VI359_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6973745.1"
                     /translation="MWRRSSRKGELLHRYWLNPIWLVLLGLSFSLGWLSVIEVGAEEE
                     PGPVARELEHIALGLKAAEKAVEKRLLSESLRLHFYGYLDASYTQNFNNPSNRINGLR
                     IFDVNSNQFRPNLAQAVLEREAKATNNWLDRIGFKVKFNVGRDSDFIGGSDLGQWADF
                     QEYYLQYIAPVGNGLNVRLGQINSLVGYEVVESPYNPNYSRSWLFGLGQPFTTRGVRA
                     SYVFNPEVSWSLGVINRINSNLPNEKGSPWVESALTLVPSDKVKLTLYGLVGPGVGAV
                     DVRWGGLLLGGGFLSFQATEQTSVVVESYYANQINSSTISSGKNARWNGVAAYLIHDF
                     STEWGIRLRSEIFEDAAGAVTCQGTTDYQPRANVCFGATSSASASAIAQTLWEVTSTL
                     QYKPISSLTTRLEYRYDKSNENVFQVGGRATSYQPTLSLEVIYLF"
     gene            complement(3652..4362)
                     /gene="urtE"
                     /locus_tag="VI359_05530"
     CDS             complement(3652..4362)
                     /gene="urtE"
                     /locus_tag="VI359_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEY6973746.1"
                     /translation="MLHLEKLNVYYGESHILRDVSFAIEPGQVACLMGRNGVGKTTTL
                     KAVMGLLPVRSGQITIGGTDVTKEATDRRVKRGLAYVPQGREIIPHLTVRENLQLGYW
                     ARHHSSNGVSEKAAFEEVYQLFPKLTQILDRPGGVLSGGEQQQLAIGRALLSDPKVLL
                     LDEPTEGIQPSIIDQIEDVINGFKNSRRFAILLVEQGLHFAARLAEKYVVMAKGAVVA
                     AGKSDELSAEMVKQHLTV"
     gene            complement(4447..5214)
                     /gene="urtD"
                     /locus_tag="VI359_05535"
     CDS             complement(4447..5214)
                     /gene="urtD"
                     /locus_tag="VI359_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY6973747.1"
                     /translation="MTERGSIIYLEGVIVDYDGFKALNNLNFIVNYNELRVVIGPNGA
                     GKTTLLDVICGKTIPAAGRVIFGKDIDLIGKREDEIVELGIGRKFQAPSIYGNLTVWE
                     NLDLSLKRASRGVFPTLLGKSTTAERERIDGNLEAIGLKDHAHTRAGSLSHGQKQWLE
                     IGMVILQDPLLLLVDEPVAGMTDKETEQTGRLLQSLAEKHAIVVIEHDMEFVRQIART
                     VTVLHEGTVICEGPVEKVQADARVREIYLGRQKVAHA"
     gene            complement(5216..>5504)
                     /locus_tag="VI359_05540"
     CDS             complement(5216..>5504)
                     /locus_tag="VI359_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973748.1"
                     /translation="AQIGVLPSLEMVIWVAVGGRGSLAGAVLGAVSVNLGRSVLTNYF
                     PELWPFILGGLFVLVVLLFPDGLVGIIRKSRSLLRPMEPGVNKVALSEGDR"
BASE COUNT         1131 a         1652 c         1445 g         1276 t
ORIGIN      
        1 aatcgtcacc cgtttttcct cccccggctc aaatcgcacc gcggtgccgg ccggaatgca
       61 gagccggaaa ccgaacgcca gcgctcggtc gaacttcagc gcgcgattgg cttcaaagaa
      121 atgacagtgg gatccgactt ggatcggccg gtcgcccgtg ttcgccacca gcagttcttt
      181 cgtcgcgcga cctgcattcg cgtcaatgtc gccggcgcca aagagaattt gtcccggcac
      241 gactggtctg aataactcgg ccttgatcat agcggccagg gagcttccct ttttctgaga
      301 ggccttgtga accttggccg gagatcgctt ggtcttcttc gccatatgcc cctcccacac
      361 agacgacaaa aatagggatg gttccgagcg ccctatcgaa tcggatcgtg cacagtcacc
      421 agtttcgtac catcggggaa ggtcccttca acctggaact cgtgaatcat ctccgctacg
      481 ccaggcatga catctttgcg cgacagcagc gtggtcccgt aggtcatgag ttcggccaca
      541 gattttccgt cacgaatgcc ttcgagaacg gcggcggtta gataggcgac tgcttcagga
      601 tggttgagct tgaggccccg cttcttgcgg tccgcggcga gttgtcctgc agtgtagatc
      661 aacagttttt cctgctccct cggggtcaga tgcatacgtt ctcctctcta tggcggacct
      721 cttgtccgtc gttcctgatt taggagaaaa ggcggaagca attgtcatta tgtattccac
      781 gtaagaaatc tattgcgggg aagaggatgc tagtctagga ttccgccacc tgtcaagacg
      841 catccctgcc gaaaggaggc cctcgtggct catgtcgcgc tcaaggatcg gtttcgggat
      901 caccctacgt ttggatttgt ggactgggtg ttgcgaggga tcgggcaggt ggtctttcaa
      961 aataatccga tctccggcgc gatcatcctc ggagcaatct tttacaattc ctggatctac
     1021 ggcgcagtct gtctgttcgg aaccatcatc agcacactga ccgctctcct gttcagggcc
     1081 gacaaaggga tgatcaaaga tggtctgttc ggatttaacg gcgcgttgat cgccattgcg
     1141 ttggtcgcct atacgagcca gaattttacg actggcgaca tgcccaatct acatctctgg
     1201 ctctacatcg tgctcagcgc cacctttacc tcagtgctca tgccagcgtt cggcgctgtg
     1261 ctggggcctc acaaagtccc gggtctcacg atgccatttg tgttggcgac ctggtttttt
     1321 ctgggcgcgt tgctgcaatt ctccaccatc gacgtatcca acgcccttaa ggcgacctcg
     1381 cccacggact ttaccggccc gcgaccggac tatacctgga tcacctggtt tcacggcatc
     1441 acgatgggca tttcggaaat tttctttcag gacaactggg tcactggagt catcatcttg
     1501 atcggcatcg caatcaacac ccgcatcggc gcattgatgg cgctcatggg atccacgctc
     1561 gcggtcgctg ccgcggtgtt gtacggcgct catgacgagg cgatccgtga tgggctcttc
     1621 ggatacaacg ccgcgttgac ggccttggcc ctcggcggcc tcttttacgt gctgaatctc
     1681 ccgggcttta tctatacggt gatcggcgtg ctggtgacgg caagggtgtg ggcttcgctt
     1741 ggtgtctttt tggaaccctc cgggatgccg gtcctgacct ccgcgttcgt atttgtcact
     1801 tggttgatgc tgctggcaaa gaacggcttc ccttcgctga tcccggtcgc accggctgaa
     1861 gccacgacgc cggaggaaaa cctcgaacga taccgggcga atcagaagag atagatgact
     1921 tcaagagaga gtgtaggctg gtagctggtg gctcggcctc cgacttgaaa gacgttctcg
     1981 ttggatttgt cataccgata ttccaggcgg gtcgtcagag aactgatcgg cttatactgc
     2041 agagtagacg tgacctccca cagtgtctgc gcgatcgcag aagcagaggc agaggacgtg
     2101 gctccaaaac acacgttggc cctgggttgg taatctgtcg ttccctggca ggtcacggcg
     2161 cctgccgcat cctcgaatat ttcactgcgt agacggatcc cccattctgt cgaaaagtca
     2221 tgaatcagat aggctgccac tccgttccac cgcgcatttt tccccgagct gatcgtgctg
     2281 ctgttgattt ggtttgcgta ataggactct actacgacag acgtctgctc cgttgcctgg
     2341 aaggagagaa aacctcctcc caagagcagt cctccccacc tcacatccac cgcgccaacc
     2401 cctggtccga ccagtccgta gagtgtgagt ttcaccttat ccgacggaac gagcgtcagc
     2461 gctgattcga cccaagggct acctttttca ttcggcaagt tactgttgat tcggttgatg
     2521 acaccaagag accacgatac ctcaggattg aacacgtagg atgctcggac accccgagtc
     2581 gtgaacggct gtccaaggcc gaacagccag gatcgtgaat agttgggatt gtatggactc
     2641 tcgacgacct cgtacccgac caggctgttg atctgaccga gtcgcacatt gagcccgttt
     2701 ccgaccggtg caatgtactg gagataatac tcctggaagt cagcccattg accgagatcg
     2761 ctcccaccga tgaagtccga atcccgaccg acattgaact tgaccttgaa gccgatacga
     2821 tccagccagt tgttggtcgc tttggcctca cgttccagga cagcctgcgc gagattcggc
     2881 cggaactggt ttgaattcac gtcgaagatg cggagcccgt tgatccgatt cgaggggtta
     2941 ttgaagttct gggtgtaaga ggcgtcgagg tacccgtaaa aatgcagcct gagactttcc
     3001 gacaggaggc gtttttcaac cgccttctca gccgccttca gccccaaggc aatatgttcc
     3061 aattcacgag caaccgggcc gggttcctcc tctgccccca cctcaatgac cgaaagccag
     3121 ccgagcgaga aagaaaggcc gagaaggaca agccagatgg ggttcaacca gtagcggtgc
     3181 agcaactcac cttttcgtga actgcgccgc cacatagatg gaggctattc ttgtttacgt
     3241 tgagtcagat gcatcgcgcc ctctctggtc ttctccctac agagggcccg aatactagcg
     3301 gggtaatcat ggtgacttca actgtgcgaa tcgagggagc actttctgaa atgcggcctc
     3361 tgtgagcatg gagccacacc tggccatctc gcctttctgt cctttcctat ttctctcgag
     3421 cggcgagcaa gaatacgttc cgccagcatc gcgaatcgca gagacgaggg caccgtcact
     3481 tagcgagcaa ctgcgttcag ccgtcggctg acggcgagga ctgtccgaag ttccgttcga
     3541 gtgtcactca gaacggaacg agttccgcag ccgagcagat gcgacgctta gtgcggtcga
     3601 gtcggagcgt ctgagcaggg ctggcgggac gcatcttgcc cctcctcatc ttcacaccgt
     3661 caaatgctgc ttcaccatct ccgcactcaa ctcatcgctc ttgcccgctg ccaccaccgc
     3721 ccctttggcc atgaccacgt acttctctgc cagccgagct gcaaaatgga gcccctgttc
     3781 caccaacaga atggcgaacc gtctggagtt cttgaaccca ttgatcacat cttcgatctg
     3841 atcaatgatc gaaggttgaa tcccttcggt cggctcatca agcaacaaca cctttggatc
     3901 ggacagcagg gcgcgcccga tcgcgagctg ttgctgctcc ccaccgctca agactccacc
     3961 cggccgatca agaatctgcg tcagcttggg aaacagctga tagacttcct cgaacgcagc
     4021 cttctcggac acgccattgg aggaatgatg ccgggcccaa tatcccaact gtaaattttc
     4081 ccgaacagta agatgcggaa tgatctctcg tccttgcgga acgtatgcga gtcctcgctt
     4141 cactcgccgg tccgtggctt ccttcgtcac gtctgttccg ccaatcgtga tctgcccaga
     4201 ccgcaccggc agcaaaccca tgacggcctt cagtgtcgtc gtcttcccca ctccgttccg
     4261 ccccatcaga caggcaacct ggcccggttc gatggcaaac gacacatccc gcagaatgtg
     4321 gctctcacca tagtagacgt tcagtttttc aaggtgcagc atggactatt gttgctcgtc
     4381 tttagtgaag cgtatctcac aattcctctc gcgcatcacg cctaacgctt tacgacttac
     4441 gtcccttcag gcatgcgcga ctttctgcct gcctaagtaa atctctcgca cgcgcgcgtc
     4501 cgcttgaact ttttcaaccg gtccttcgca aatcaccgtc ccttcgtgaa ggaccgtgac
     4561 cgtcctggca atttgtcgga cgaattccat gtcgtgctcg atgacgacga tcgcatgttt
     4621 ttccgccaac gattggagta accgccccgt ctgttcggtt tctttgtccg tcatgcctgc
     4681 gaccggttca tcgaccagga gaagcaaggg atcttggaga atcaccatcc cgatctcaag
     4741 ccactgtttt tgaccgtgcg agagcgatcc ggctcgtgtg tgtgcatgat ccttgagtcc
     4801 aatggcttca aggttcccgt cgatccgttc ccgctcagcc gtggtggatt ttcccagcaa
     4861 cgtcgggaag acgcctctgc tggcacgttt taatgacaga tcgagatttt cccagaccgt
     4921 gaggtttccg tagatcgacg gtgcctggaa cttcctcccg ataccaagct ccacaatctc
     4981 atcctcccgt ttgccgatga gatcaatatc cttgccgaaa atcacacgac cggcggccgg
     5041 gatcgtctta ccgcagatca catcgagcag cgtcgtttta ccggcgccgt tcgggccgat
     5101 cacgacccgc aattcgttgt agttgacgat gaagttaaga ttgttgagcg ctttaaagcc
     5161 gtcgtaatcg acaatcacgc cttcgagata aatgatggac cctcgttcgg tcatgtcagc
     5221 ggtctccctc cgacagggcc actttgttca cacctggttc catcggccgc aggagactcc
     5281 tgcttttcct gattatgccg accagcccgt cggggaacag caggaccacc aacacgaaga
     5341 gccccccgag tatgaacggc cagagttcag ggaaatagtt cgtgagcacg ctgcgaccga
     5401 ggttcacgct gaccgctccc agcaccgcgc cagccagact gccacgccca ccgaccgcca
     5461 cccaaatcac catttcgagt gagggcagga caccgatttg cgcc
//
LOCUS       DATDNW010000223         2919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_300517, whole genome shotgun sequence.
ACCESSION   DATDNW010000223 DATDNW010000000
VERSION     DATDNW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300517"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="tgt"
                     /locus_tag="VI359_05545"
     CDS             complement(<1..1098)
                     /gene="tgt"
                     /locus_tag="VI359_05545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY6973749.1"
                     /translation="MTGFSVTQIDQATCARRGTLITAHGQIDTPMFMPVGTLGPVKGV
                     DPDDLQQLGFRLMLNNAYHLYLRPGHKVVAELGGLHAFTGWPGAILTDSGGYQIFSLA
                     KLCRVTDEGVSFQSHLDGSTHFITPETAIEIEEALGADIVMALDQCVALPASPDAMRD
                     GVRRTKLWAERCQTSRRRTDQALFGIVQGGLDAELRVAAARELVGLGFDGYAIGGLSV
                     GESKDEMYAMLDVTVPELPVSKPRYLMGVGMPENLVEGVARGIDLFDCVVPSRHGRTG
                     WLFTNFGRVLIRQAQYVRDDRPVDPACGCPVCLRYSRAYLHHLFNVKEMLGSRLNTIH
                     NLWYFADLMRRIRVAIEQGTFSAFRAEFYRSR"
     gene            complement(1113..2867)
                     /gene="argS"
                     /locus_tag="VI359_05550"
     CDS             complement(1113..2867)
                     /gene="argS"
                     /locus_tag="VI359_05550"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY6973750.1"
                     /translation="MSQGVVQEKVANALLGALNGAKQKGQLKTDIWPQLSLDAPKRPE
                     WGDLASTVAMSLAGPEQRAPYDIAQIIVDNLPERELLFDRVEIVRPGFLNLTIKPLIW
                     QEVLREIEGQGFAYGKADLGKGQRVLVEYVSANPTGPMHVGHGRGAAVGQALARLLKA
                     VGYDVISEYYINDAGRQVKLLGASVYARYQELAGRPAEFPPDGYHGSYVKALAERVKQ
                     HFGARLDTLPPAEAEARCRDFAYQEMLVIIRRDLTAFGIEFDSWFSEASLLASQTVER
                     VFAELKAKGLLFEQEGAQWFRSSAFGDEKDRVVRKQDEEYTYLASDIAYHRDKLQRGY
                     DLLVDVWGADHHGYIPRMQAVMQAYGYPKDRLRVVLVQMVNLLRGGKKVEMSKRAGEF
                     ITLREVIDEVGADAAKFFFLMRDSNTHLDFDLELAKQRSADNPVYYVQYAHARIASLW
                     RVAASRGVACPLPSETELALLTDPDELGLIRKLSAYPAVLQGTALAFEPHRMTYYLQQ
                     LAAGLHTFYNKHRILPPAADNDMIDTTPEGLVPTERRLKEELTPQRTAARLALMRGVQ
                     QVLKNGLGVLGISAPEQM"
BASE COUNT          639 a          914 c          779 g          587 t
ORIGIN      
        1 acgactccgg tagaactcgg ctcgaaacgc gctgaaagtc ccttgctcaa tcgcaacccg
       61 gatgcggcgc atgagatcgg cgaaatacca gaggttgtga atcgtattga gccttgagcc
      121 gagcatttct ttcacgttga acagatgatg taagtacgcc cgcgaatacc gcaggcagac
      181 cggacagccg caggcaggat cgaccggcct gtcgtcacga acatattgag cctgtctgat
      241 caggacacgg ccaaagttgg taaagagcca gccggtccgg ccgtggcgtg atggcacgac
      301 acaatcgaac agatcaatgc ctcgggcaac gccctcgacg agattttcgg gcataccgac
      361 gcccatgaga taccgtggtt tcgaaaccgg tagttccggc accgtgacat caagcatggc
      421 gtacatctcg tccttggact cgccaacaga cagacccccg atagcgtatc cgtcaaaccc
      481 gagaccgaca agctctcgcg ccgcggccac acgcagctcc gcgtctagtc ctccttggac
      541 aattccgaaa agcgcctggt cggttcggcg acggctggtt tggcaccgtt cggcccagag
      601 tttcgtgcgt cgcacaccgt cacgcatcgc atcaggagaa gccgggagcg cgacacattg
      661 atcgagcgcc atgacgatat cggcacctaa ggcttcttcg atctcgatgg ccgtttctgg
      721 cgtgatgaaa tgcgtggaac cgtcgagatg agactgaaaa ctgaccccct cgtccgtcac
      781 tctgcataac ttcgccaagc tgaaaatttg atatccgccg ctgtccgtca gaatcgcccc
      841 gggccacccg gtgaacgcat ggagcccgcc taattctgcc acaactttat gtcctggccg
      901 cagatacaga tgataggcgt tgttcagcat caaccgaaat ccgagttgtt ggaggtcgtc
      961 cgggtcgacc cccttgaccg gacctaacgt cccaaccggc atgaacatcg gtgtatcgat
     1021 ttggccgtga gccgtgatca gcgtccctcg ccttgcacag gtcgcctgat ctatctgcgt
     1081 aacagaaaag ccagtcatac gttctcgctc aactacatct gctcgggcgc cgaaatcccc
     1141 agcaccccca agccgttctt cagcacttgc tgcacacccc gcatcaaggc cagccgtgca
     1201 gctgtccgtt gaggtgtgag ttcctccttc aggcgtctct ctgtcggcac caagccctct
     1261 ggcgttgtat caatcatgtc gttatccgca gccggaggga ggatccgatg cttgttgtaa
     1321 aacgtgtgca ggccggcggc caattgctgc agatagtagg tcatgcgatg tggctcaaag
     1381 gcgagcgccg ttccttgcag cacggcggga taggccgaga gtttgcggat cagccccagc
     1441 tcatcgggat ccgttaagag cgccaactcc gtttcgctcg gcagcggaca ggcgacccct
     1501 cgtgaggcag ccacccgcca caagctcgcg attcgagcat gcgcgtactg cacgtaatac
     1561 acgggattgt cggccgagcg ttgcttggcc aactcgaggt cgaagtccaa gtgcgtattg
     1621 gaatcccgca tcaagaagaa gaactttgcg gcgtcggctc ccacctcgtc gatgacttcg
     1681 cggagcgtaa tgaactcgcc cgcacgtttc gacatctcca ccttcttccc gccacgcaga
     1741 agattcacca tctgcaccaa gacgacgcgc aggcggtcct tgggatagcc gtaggcttgc
     1801 atcactgcct gcatccgggg aatatagcca tgatggtcgg ctccccagac gtcgacgagc
     1861 agatcgtacc cccgctgcaa tttgtcacgg tgataggcga tatccgaagc gaggtaggta
     1921 tattcctcat cctgcttacg gaccactcga tctttctcat ccccgaacgc agacgatcgg
     1981 aaccactgtg cgccctcctg ttcgaacagc aggcccttgg ccttcagctc agcgaatact
     2041 cgttcgacgg tctgtgacgc gagcagcgaa gcctcgctga accatgagtc aaactcaata
     2101 ccgaacgccg tcaggtcccg gcgaatgatc accagcatct cctggtacgc aaagtcacga
     2161 cagcgagcct cggcttcagc gggaggcaag gtgtcaagac gagcaccaaa atgctgtttc
     2221 acccgttccg cgagagcctt gacatagctg ccgtgataac cgtcaggagg aaattccgcc
     2281 ggccgaccgg ctaattcttg ataccgtgca tacacggagg cgcccaggag tttcacctgt
     2341 cgacccgcgt cgttgatgta gtactcactg atgacgtcat atccgacagc cttgagaagt
     2401 cgtgccaggg cctgtcctac cgctgcccca cggccatgac ccacatgcat cggccccgta
     2461 ggattggcac tgacgtactc caccaacact cgctgcccct ttcccaaatc agccttccca
     2521 taggcaaacc cctgtccttc gatctctcga aggacctctt gccagatcag cggcttgatc
     2581 gtgaggttaa ggaatccagg tcgcacaatc tccacccgat cgaatagcag ctcacgctcg
     2641 ggaagattgt cgacaataat ctgggcgatg tcgtaaggag cacgctgttc gggaccggcc
     2701 agcgacatgg ccaccgtgga ggcgagatca ccccactcgg gacgcttggg tgcatccaaa
     2761 ctcagttgcg gccagatgtc ggtcttgagt tgtcccttct gcttcgcccc attgagggca
     2821 cccaggaggg cattggctac cttctcttgt acaacccctt gagacactgc aaaccgctcc
     2881 taagtcctta ccaaagcaag ggaaaaccaa ttgggcact
//
LOCUS       DATDNW010000224         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_913200, whole genome shotgun sequence.
ACCESSION   DATDNW010000224 DATDNW010000000
VERSION     DATDNW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913200"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VI359_05555"
     CDS             complement(<1..518)
                     /locus_tag="VI359_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY6973751.1"
                     /translation="MSKTYDHHAIEAKWQAYWEQHRPFRAVDDRSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMKGFNVLHPMGWDAFGLPAEQYAVKTGVHPAVTT
                     AQNIETFKRQMKRVGLSYDWDRELSTTDPDYYRWTQWIFLKLYERGLAYVAEVPVNWC
                     PALGTVLANEEIV"
     gene            689..1306
                     /locus_tag="VI359_05560"
     CDS             689..1306
                     /locus_tag="VI359_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973752.1"
                     /translation="MGLYSYYIFPCLMDWVMSGDEFQRWRAVVLQDVRGEVLEIGLGT
                     GLNLPHYPETVSLLHAVDPSPLLPERVARRSKSAAFPIHITQISAETLPFGERRFDCV
                     VSTWTLCTIPDPVKALQEIRRVLKPSGRFLFLEHGRSENEKIAAWQNRLNPIQRIVGC
                     GCNLNRRIDQFITQAGLSITKLDRFQMEGIPRLVGEMYRGVAVSA"
     gene            complement(1303..2001)
                     /locus_tag="VI359_05565"
     CDS             complement(1303..2001)
                     /locus_tag="VI359_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY6973753.1"
                     /translation="MTRHSALSPQHYLNWKEIDDVLLDMDGTLLDRHFDNFFFEEELP
                     KRYALLHGMVFEEARDLLMSMYRSVEGELAWTDLHYWTKRVGIDVVAMTKEFDHLIGF
                     LPGAEEFLQNLRRMGKRVTILTNAHGSGVAIKTARTGVDQCVDRVVNAFEVGYLKMRP
                     EYWPNCQRLVGFEPERSLYVDDDEGCLAAAQDFGVAHLLHSAKSSSQLAPTRSSLFTS
                     IEHFSVLMNGHLGR"
     gene            2086..>2421
                     /locus_tag="VI359_05570"
     CDS             2086..>2421
                     /locus_tag="VI359_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY6973754.1"
                     /translation="MARTKRKPSSAPTLPMPDTGRIPIVAIIGRPNVGKSTLFNRILG
                     SRTAIVDDVPGVTRDRNYADATYRDRKFRLVDTGGLEPSATDGMLALIRRQSELAIAE
                     ADILILLMDG"
BASE COUNT          532 a          690 c          677 g          522 t
ORIGIN      
        1 acgatttctt cattcgccag cacggtaccc agcgccggac accagttgac cggcacctcc
       61 gccacatagg cgagaccccg ctcgtagagt ttcaagaaga tccattgtgt ccaacgataa
      121 tagtccggat ccgtcgtact gagctctcga tcccaatcat aggaaagccc gacgcgtttc
      181 atttgtcgtt tgaaggtctc gatattctgc gcggtcgtca cggccggatg tacgcccgtt
      241 ttcacggcat actgttcggc cggaagccca aacgcgtccc atcccatcgg atggagcacg
      301 ttgaacccct tcatgcgctt atagcgggag acgatgtcgg tggcggtata gccttcaaga
      361 tgaccgacgt gcagtccgga tccggaggga tagggaaaca tgtcgaggca ataaaacttg
      421 ggcttcgacc ggtcatccac tgcccgaaac ggccgatgct gttcccaata ggcttgccat
      481 ttcgcctcga tggcgtggtg atcgtaggtt ttgctcatga taatgcacgg tagcgatcgg
      541 cccttaactt gatccttcta tcaaagtgat ccatgaaggc cgaccaccga cggcctcttt
      601 gcaatcagcg aatctaacat agcgcgtaga gggtcacaag attcgcaagg ggccgttcgt
      661 ctcgactttg ctatactgcg ggcgactcat gggactctat agctactaca ttttcccctg
      721 tcttatggat tgggtcatga gcggggacga atttcagcgg tggcgagcgg tggtgctcca
      781 agacgtgcgg ggtgaagtat tggagattgg tctgggaacg gggctgaatc tcccccacta
      841 ccccgagacc gtgtcattgc tgcatgccgt ggatccgtca ccgctcctgc ccgagcgcgt
      901 ggcgcggcgg agcaagtctg ccgcgtttcc gattcacatc acacaaatca gcgcggaaac
      961 tctcccgttt ggcgaacgta gatttgactg cgtggttagc acgtggacgc tctgcaccat
     1021 tcccgatccg gtcaaagcgc tccaggagat ccgccgcgtg ctcaaaccat ccggccgatt
     1081 tctctttctt gaacatggcc gaagtgagaa tgagaagatc gccgcgtggc agaatcggct
     1141 caatccgatc caacgaatag tcggatgcgg gtgcaatttg aaccgacgga tcgatcagtt
     1201 catcacgcag gccggtctct ccatcacgaa actcgatcgg tttcaaatgg agggcatccc
     1261 gcgactggtc ggggaaatgt accggggcgt ggcggtctcc gcctagcgac cgaggtgtcc
     1321 gttcatgagg acagaaaagt gttcgatcga ggtaaacaga gacgaacgag tcggggcgag
     1381 ttgggagctg gattttgcac tgtgcaagag atgagcgaca ccaaaatcct gggcggcggc
     1441 cagacaaccc tcgtcgtcat cgacatagag tgagcgctct ggttcgaacc ccacaagccg
     1501 ttgacaattg ggccaatact ccggccgcat ctttaaataa cccacctcaa acgcgttgac
     1561 cacccgatcg acgcattgat ccacgccggt tctcgccgtc ttgatcgcca cacccgaccc
     1621 gtgggcgttc gtcagaatcg tcacccgctt gcccatccgt cgcaggtttt gcaagaactc
     1681 ttccgcccct ggaagaaatc cgatcaggtg atcgaactcc ttcgtcatgg ccacaacgtc
     1741 gatgccgacg cgcttcgtcc agtaatggag gtcggtccag gctaactcgc cttcgactga
     1801 ccggtacatc gacatgagca ggtcgcgggc ttcttcgaag accatgccat gtaacaaggc
     1861 gtaacgcttc ggcaattcct cttcgaagaa gaaattatcg aaatggcggt cgagcaatgt
     1921 accgtccata tcgagcagga cgtcgtcgat ctctttccaa ttgagatagt gctggggact
     1981 gagtgctgag tgccgagtca tgggaagagt atagcgaaga aggaccgaca agacgagact
     2041 gttgtctttg tgaaaatccc tgtgttacgg tccgcgcgag gttctatggc gcgcaccaaa
     2101 cgcaagccct cttctgcccc gacgctgccg atgcccgata cagggcgcat accgatcgtc
     2161 gccatcatcg gtcgaccaaa tgtcgggaag tccacgctct tcaaccgtat cctcggcagc
     2221 cggactgcga tcgtcgacga cgtaccgggc gtcacacgag accgtaatta tgccgatgcc
     2281 acctaccgtg atcgaaagtt tcggttggtt gacacagggg gactcgaacc ctcggcaaca
     2341 gacgggatgc tggcgttgat ccgccgtcag tcggaattgg cgattgccga ggctgatatc
     2401 ctcatcctct tgatggatgg a
//
LOCUS       DATDNW010000225         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1189739, whole genome shotgun sequence.
ACCESSION   DATDNW010000225 DATDNW010000000
VERSION     DATDNW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189739"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..>3006)
                     /locus_tag="VI359_05575"
     CDS             complement(3..>3006)
                     /locus_tag="VI359_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973755.1"
                     /translation="KWNGNVAVGERESTAQNPKYKWAAKWDDSTRPAILFDPTTGKMA
                     WPLFKPHFGKRVPFSSNHSGAPWLEPIHQDANGERTSEPAKPGEQGRWSLCPQNANQK
                     FYNIHFVRMPITLAKQQGKEPAIVDKDGLIYVLHEEERLTRANDDLKFPAVIRANVYD
                     CVDLVLTSEWNDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGFEYEMSVRPFTMWGK
                     GKKHGLPAPMVAKIVGGATAGASSIKIQMAPGATPFHVNTELMVGMDCLEKGNDPAAS
                     LPRDKSCSEIARIKEIKGDQVTFFKPLKQNHPAGDLVSPEFVRYRWWVDVDMGTVFWH
                     DHAFGATTWPHGGFGVTIVEPFGSTYHNPKDGKLVYSGPIADIHSNEPIGAGVSGSFR
                     ELMVSIHDTVPHTVNVIQAGNPPGQPVEVALEAGKTVSFQMPEKILNAPNQFLNGGTH
                     TTGSGFNFRAEPFAQRLSNNPDTSKLFSSVVHGDPDTPLLRAYTGDTMVFRLLHQLMN
                     ESHVWTIAGHTFLTERYAADANRKNSIHVGIAERYDLVTKAGGFQGMPGDYIHFNGRS
                     SHFAEGGWGIVRVLDNETADLKPLPRGTNPLGIPATPSGVCPADAPVKSFNVVSLDRP
                     LKLHPKAPEAIEVDFERKIELTVPEGKIFALEEEATTVAGNVMPNPLTLRANLGDCIK
                     VTLKNKMKASRASFFAPGLAFDPKDSQGLNVGNNGGDQTIGPGESRNYTYYAHPANKE
                     TTSLVWDGGNVVVNPRNGLYGAIIIGPKGSQYRDPVTGADLSQKNAWRADVIVDASLP
                     ENVGKRNYRDVALFFQDEDNIIGTSFMPYVQNVAGLTSVNYRAEPYKYREEQGCSLGK
                     IFQPCVVDKPEDPATPLIEAHAGDPIRIHVIGANSEQNGMFGVEGHEWPIEPYMPGAD
                     MISVVEYAGSETLDVFIRGGAGGPYRLTGDFVWSNNRLPYAQSGQWGYLRVLPTGDSR
                     IQPLGAASMGAKRADAQPDAKAIPTAMK"
BASE COUNT          563 a          937 c          828 g          678 t
ORIGIN      
        1 acctacttca tcgcagttgg aatcgccttc gcgtcgggct gcgcatccgc ccgcttggca
       61 cccatgctgg ccgcgcccaa cggttggatc cgtgaatcac cggtcggcaa cacacggagg
      121 tagccccatt gcccggattg cgcgtaaggc agtcggttgt tggaccacac gaaatctcca
      181 gtcagacgat atggtccgcc cgcaccgccg cggatgaaga catcgagcgt ttctgaaccg
      241 gcatactcta cgacactgat catatcggcc cccggcatgt agggttcgat cggccactca
      301 tggccctcta caccgaacat cccgttctgc tcgctgttgg ccccgatcac gtggatgcgg
      361 atcggatcgc cggcgtgggc ctcgatgaga ggcgtggccg gatcttcggg cttatccacg
      421 acgcagggct ggaaaatctt ccccagcgaa cagccttgct cttcacgata cttgtacggt
      481 tcagcgcggt agttcaccga cgtcaaaccc gccacgttct gcacataggg catgaacgag
      541 gtcccaatga tgttgtcctc gtcctggaag aacagtgcga catcgcgata attccgcttg
      601 ccgacgttct ctggcaggct ggcatcgacg atgacgtccg ctctccaggc attcttctgc
      661 gagagatccg caccggtgac gggatcacga tactgcgatc ccttcgggcc gatgatgatc
      721 gcgccataca atccgttacg cggattgacg acgacgttgc cgccgtccca caccaacgac
      781 gtcgtttcct tattcgccgg atgcgcgtag taggtgtagt tgcggctttc gcccggtccg
      841 atggtctgat cgccgccatt gttgccgacg ttcaggccct ggctatcctt tggatcgaaa
      901 gctaatccgg gcgcaaagaa cgaggcccgg ctcgccttca tcttgttctt caacgtgacc
      961 ttgatgcagt cgccgaggtt cgcgcgcaac gtcaacggat tcggcatgac attacccgcc
     1021 accgtcgtgg cttcttcttc gagcgcgaag atcttgcctt ccggtacggt cagctcgatc
     1081 ttgcgttcaa aatcgacttc gatcgcctcc ggcgccttcg gatggagctt cagcgggcga
     1141 tccaacgaca ccacattaaa gctcttcacc ggagcatcgg ccgggcacac tccactcgga
     1201 gtggctggaa tgccgagcgg attggtgccc ctcggcagcg gcttgagatc cgccgtttcg
     1261 ttatcgagca cccgcacgat accccatcca ccttcggcga agtgcgagct tcggccgttg
     1321 aaatggatgt agtcacccgg catgccctgg aatcctcccg ccttcgtcac gaggtcgtac
     1381 cgttcggcaa tcccgacgtg aatcgagttc ttccgattgg catccgccgc ataccgttcc
     1441 gtaaggaaag tatggccggc gatcgtccag acatgcgact cgttcatcaa ctgatgcagc
     1501 agacggaaga ccatcgtgtc gcccgtgtac gcccgcaaca acggtgtatc gggatcacca
     1561 tggaccacgc tgctgaacag ttttgacgtg tccggattat tcgagaggcg ttgggcaaac
     1621 ggttcggcgc ggaagttaaa tccgctgccg gtcgtgtgcg ttccgccgtt caggaactgg
     1681 ttcggagcat tcagaatctt ttccggcatc tggaacgaca ccgtcttacc cgcttccagc
     1741 gccacttcaa ccggctgtcc gggaggatta ccagcctgga tgacgttcac cgtgtgcggg
     1801 accgtgtcat ggattgacac catcagctca cggaagctgc cgctcacacc ggcgccgatc
     1861 ggttcattcg aatgaatgtc cgcgatcggc ccactgtata ccagcttgcc gtccttcgga
     1921 ttgtgatatg tggacccgaa tggttccacg atcgtcacgc cgaacccgcc atgtggccag
     1981 gtcgtggcgc cgaatgcatg gtcatgccag aagaccgttc ccatatccac gtccacccac
     2041 caccgatagc ggacgaattc cggcgacacg agatcgcccg ccggatggtt ctgcttcagc
     2101 ggcttgaaga acgtcacctg atcgcccttg atctccttga tcctggcgat ttccgagcaa
     2161 ctcttgtcac gcggcagtga ggctgctgga tcattgccct tctccaaaca atccatgcct
     2221 accatcaatt cggtgttcac atggaatggc gtggcgccgg gcgccatttg gatcttgatg
     2281 gagctggctc ccgccgtcgc tccgccaacg atcttggcca ccattggtgc aggcaatcca
     2341 tgtttcttgc ccttgcccca catcgtgaac ggccgaaccg acatttcata ttcgaatcct
     2401 gagatcacac cgtccgagtt ccccgtatcg aactggaaga aatggggatg gatgttgatc
     2461 ttggatgact ggaagttcgt gtagtcatcg tcgttccatt cgctcgtcag caccaggtcc
     2521 acacagtcgt agacgttggc gcggatgacc gccgggaact tgagatcatc gttggctctg
     2581 gtcaagcgct cctcctcatg gagcacatag atcaggccgt ccttatccac gattgccggc
     2641 tctttgccct gctgtttggc aagagtgatc ggcatgcgga cgaagtggat gttgtaaaac
     2701 ttttgattgg cattctgcgg acagagactc caacgtccct gctcgcccgg cttggccggc
     2761 tcagatgtcc gctccccatt ggcatcctgg tggatcggct ccaaccaggg tgcgccgctg
     2821 tggttcgatg agaatggcac gcgctttccg aagtgcggtt tgaacagcgg ccatgccatc
     2881 ttgcccgtcg tcgggtcaaa gagaatggcc ggcctggtgc tgtcatccca tttcgccgcc
     2941 cacttgtact tgggattttg cgcggtgctt tcacgctcgc ccaccgccac gttgccattc
     3001 cacttc
//
LOCUS       DATDNW010000226         3613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_450696, whole genome shotgun sequence.
ACCESSION   DATDNW010000226 DATDNW010000000
VERSION     DATDNW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450696"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VI359_05580"
     CDS             <1..1053
                     /locus_tag="VI359_05580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY6973756.1"
                     /translation="GSADLSYVEEVGRGIARSMTGYKVIVTKSTVPVGTGETLRKVIT
                     ASQSDKFRFDIVSNPEFLREGSAIEDFMRPNRVIIGAESEQAVAIMKDLYRPLYLIET
                     PFVVTDIPTAEMIKYASNAFLATKISFINEMAALCEKVGADVQLVAKGMGLDKRIGSK
                     FLHAGPGFGGSCFPKDLAALIQTGERLGYSVQIAKAASQVNDEQRQRMVEKIRETVGV
                     LKGKTFALLGLSFKPNTNDLREAPALAIAQILMKQGATIRAYDPVALEEASRTLNGLI
                     PCRDIYHAAEGSDALILMTEWNQFRTLDFERLKTLMRKPVLFDLRNVYEPAKVAACGF
                     LHVSVGRPSKNPSPSA"
     gene            complement(1070..1777)
                     /locus_tag="VI359_05585"
     CDS             complement(1070..1777)
                     /locus_tag="VI359_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6973757.1"
                     /translation="MADEAPAKLIQIGPKGGLKKDGFNLVTERVVAVNPEAKQLEVEL
                     LAYDGKTVLLDVAEEALEDLKQIKAGDGATIRVVEEGGRRIAKSFKIRAKDPNAAKAD
                     AMLLDLKDPHWLNRKYAAEILGELKEARAVEPLVEALTDDVGDVRQRAYDSLIKIGGS
                     AVPSLVPLLVSEEDELRQSVTEIIRKIGKPAVEPLATALAEADDRLKTRIMKVLDRMG
                     YKPKPKDEAKAEIPRLT"
     gene            complement(1810..2571)
                     /locus_tag="VI359_05590"
     CDS             complement(1810..2571)
                     /locus_tag="VI359_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6973758.1"
                     /translation="MNDETRTDRIGQLISALRDDNEALRDHAIASLGQIGEEAVPQLI
                     GLMADEDMLIREAATSAVVRIGPSVVDRLIEALTDDEWAVREQAASGLGKLKDPRGVD
                     PLVVALKDKDGAVRTAAVWALERIGDSRAVPGLIEALTDSTLREDVARVLKKIGDTRA
                     VDALIDGLMGNNWMVRRHAAEALGKIGDQRGIPPLIESLKDEDWLVRRNAAESLARLG
                     AKQAIDPLLPLLEDENTMVQETVEGVLASLGWKAK"
     gene            complement(2564..>3613)
                     /locus_tag="VI359_05595"
     CDS             complement(2564..>3613)
                     /locus_tag="VI359_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6973759.1"
                     /translation="DWRRMRATAACLSGGPRAVHALVHAVETGVPALKVEAAAMLARV
                     KDPQAGVPLVKLLRETDEQVQRAGASALEQMAGVLDRDTASALVSLLYEAKDERVRTV
                     ASQLLGAIPNAVPSLCEMMKHPDPDAQIMAATMLEQLLDPRSADAFIDAMGQPAVRET
                     AVRTLKKLGAIRECIDQRFNALRDVEGASEREEARMATVIELLNIGRPSVEILIEYLE
                     DDDWLVREAAADLLGKIGDVRAVGPLMKRLEVDKDTGVKELALKSLGLIGDARPAELY
                     IEAIPIRPLRVYAMEALAKVKDVDVLRPHRELFERLRTDRDGLVAYNAGLIADKLAAL
                     DGEQAASEEGLHHDE"
BASE COUNT          706 a         1159 c          953 g          795 t
ORIGIN      
        1 gggtccgccg acttgtctta tgtcgaggaa gtcggtcgtg gaatcgctcg aagcatgaca
       61 ggctacaaag tcatcgtgac caagtcgacg gttccggttg ggaccggaga gaccttgcgt
      121 aaggttatta cggccagcca gtctgacaag tttcggttcg atatcgtgtc aaatccggaa
      181 tttctccgtg aaggatcggc catcgaagac ttcatgcgac caaaccgtgt gatcattggg
      241 gcggagagtg aacaggccgt ggccatcatg aaagacctct atcgccccct ctatttgatc
      301 gagactccct tcgtcgtcac ggacatcccg accgcggaaa tgatcaaata tgcatcgaac
      361 gccttccttg cgaccaaaat ttcgttcatc aatgagatgg cggcgctttg cgaaaaagtg
      421 ggtgctgatg tgcaactcgt cgcgaagggg atggggctgg acaagcgcat cggatccaaa
      481 tttctccatg ccggacccgg cttcggtggt tcgtgcttcc cgaaggatct tgccgctctg
      541 atccaaaccg gagaacggct cggctactcc gtgcagattg cgaaggctgc gtcacaagtc
      601 aatgacgagc agcggcagcg gatggtggag aaaatccgcg aaacggtcgg ggttctgaaa
      661 ggaaaaacct tcgctctatt gggtctgtcc tttaaaccaa acacgaacga tttacgcgaa
      721 gcgcccgcgc tagcgatcgc acagatcctg atgaagcaag gggctacgat tcgcgcctat
      781 gacccggtcg ccctcgaaga agcgtctcgt accttgaacg gtctcatacc ctgtcgggat
      841 atttaccacg ccgctgaggg gagtgacgcg ctgattctca tgacggaatg gaatcagttc
      901 cgaaccttgg atttcgaacg actgaagacc ctgatgcgca agccggtgct cttcgatctt
      961 cggaatgtct atgaaccagc caaagtcgca gcctgcggat ttctccacgt gtcggtggga
     1021 cgccccagca agaacccttc gccgtctgcc taaccgcctt gaccgagcat taggtgagtc
     1081 ggggaatttc ggccttcgcc tcatccttcg gtttcggctt gtaccccatt cggtccaata
     1141 ccttcatgat ccgagttttt aaccggtcgt cggcttcggc aagagcggtg gcgagaggtt
     1201 ccaccgccgg cttcccgatc ttccggatga tttcagtgac ggactgccgc agttcgtctt
     1261 cttccgacac taagagtggc acgagagacg ggaccgccga tccaccgatc ttgatcaagg
     1321 agtcgtaggc ccgctgccgg acgtcgccca catcatccgt caacgcctcg accaacggtt
     1381 ccacagcacg cgcttctttg agttctccca ggatttccgc cgcatacttt cgattgagcc
     1441 aatgcggatc cttgagatcc aacagcatgg cgtcggcttt ggcggcgttg gggtctttcg
     1501 cgcggatctt gaagcttttt gcaatccggc ggccgccttc ctccacgacg cggatcgttg
     1561 ccccatctcc ggccttgatc tgcttcaggt cttcaagcgc ttcttcggca acatccaaca
     1621 gcacggtctt cccgtcgtag gccaacagtt cgacttcaag ttgttttgcc tcaggattga
     1681 cggccacaac ccgttccgtc accaggttga atccgtcttt cttgaggccc cccttgggac
     1741 caatttgaat gagtttcgct ggagcttcgt ccgccatgat gtgattcctt tacgtccaaa
     1801 aagagatgat tactttgctt tccaccccaa actcgccaac accccttcga ccgtctcctg
     1861 caccatcgta ttttcgtctt ccagcaacgg aagcaatgga tcgatcgcct gcttggcgcc
     1921 caatcgcgca agcgactccg ccgcgtttcg acgcaccaac cagtcctcgt ccttgagcga
     1981 ttcgatcaac ggcgggatcc ctcgttgatc cccgatcttg cccaatgcct ctgctgcatg
     2041 ccgccgcacc atccaattgt tccccatcaa cccgtcgatt aacgcgtcca ccgccctcgt
     2101 gtcaccgatt tttttcagta ccctcgcgac atcctcacgt aacgtactgt cggtcagggc
     2161 ctcgatcaac cccggcacgg cacgggagtc gccgatccgc tcgagcgccc aaacagcggc
     2221 ggtcctgacc gctccgtctt tatccttcag cgccacaacc agcggatcca ccccgcgcgg
     2281 gtccttgagc tttcccagcc ccgatgccgc ttgttcccga acggcccatt cgtcatcggt
     2341 gagggcttcg atcaatcggt cgactactga gggaccgatt cggacgacgg cgctggtcgc
     2401 cgcttctcga atcaacatat cctcgtccgc catcagacca atgagctgag gcacggcctc
     2461 ctcgccgatc tgcccgagac tggcgatggc atggtcacgg agcgcctcgt tgtcgtcgcg
     2521 caaggctgat atcaactgtc caatgcgatc cgtccgggtc tcgtcattca tcgtggtgca
     2581 atccttcctc actcgccgct tgttctccgt cgagcgctgc gagcttatcg gcaatcaagc
     2641 cggcattata cgccaccaaa ccgtcgcggt cggtccgtag tcgttcgaac agttcccgat
     2701 gcgggcggag aacatccaca tccttcacct tggcaagcgc ctccatcgca tagacccgga
     2761 gcggacggat gggaatcgcc tcaatgtata attcagccgg tcgagcatcc ccgatcagtc
     2821 ccagtgactt caaggccaat tctttgaccc cggtatcctt gtcgacctcc agccgtttca
     2881 tcaaggggcc gacggcacgg acgtcgccga ttttccccag caaatcagcc gcggcttcgc
     2941 gcactaacca atcgtcgtcc tcaaggtact cgatcaaaat ttccacgctg ggacgaccga
     3001 tattgaggag ttcgatgacc gtcgccatgc gggcttcctc tcgttcactg gcgccttcga
     3061 cgtcgcgcag agcgttgaat cgttgatcga tacattcgcg gatggccccc aattttttga
     3121 gcgtgcgcac agccgtctcg cgcaccgccg gttgccccat cgcgtcgatg aatgcatccg
     3181 cggagcgggg gtcgagtagc tgttcgagca tcgtggccgc catgatctgc gcatccgggt
     3241 caggatgctt catcatctcg cacagcgagg gaaccgcgtt ggggattgcc cccagcaact
     3301 gcgaggccac cgttcgcacc cgctcgtcct ttgcttcata caacaaggac accagcgccg
     3361 acgccgtgtc tcgatccagg actcccgcca tttgctccaa tgcggatgcg ccggcacgct
     3421 gaacctgttc gtcggtctca cggagcaact tgaccaacgg cactccggcc tgcggatctt
     3481 tgacgcgcgc caacatggcc gccgcctcca ctttcaaggc cggaacgcct gtttccacgg
     3541 catgcacgag agcatggacc gcccgcggtc caccagacaa gcaggcggct gtggcccgca
     3601 tccgccgcca gtc
//
LOCUS       DATDNW010000227         4653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_370876, whole genome shotgun sequence.
ACCESSION   DATDNW010000227 DATDNW010000000
VERSION     DATDNW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370876"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..484)
                     /locus_tag="VI359_05600"
     CDS             complement(2..484)
                     /locus_tag="VI359_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973760.1"
                     /translation="MAGLMSADEIFKKAEDAAVAATGPDEKALQIDYQALKEKIRAAL
                     GDRKVALCHINKFLPEGYEEQGRFNLMLLTAGNVVFDMVIGDSYFRYDIVSVGQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDEERKSLLAFARAVAAVRNPER
                     "
     gene            complement(497..799)
                     /locus_tag="VI359_05605"
     CDS             complement(497..799)
                     /locus_tag="VI359_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973761.1"
                     /translation="MGVVVHMVLQIVSVMGALMVLVAYGLIQSGVWRELDRGYLAFNI
                     VGSLLLGIVAIADQRMGFILLEFAWAGLGIVGVVRAIKARSASENVSQNMSCCTTK"
     gene            complement(925..2298)
                     /locus_tag="VI359_05610"
     CDS             complement(925..2298)
                     /locus_tag="VI359_05610"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY6973762.1"
                     /translation="MPSAATSKSLPQGIAIGDVTSQSALLWLRTDGPMRVQVEWASVA
                     AWEAVSKQATAIAHVARTPLFMTGPETDYTLTIPFLGLMPATAYRYHILVGPKGDEGQ
                     HIEARVEAKGEFTTLPDAKTSAPVTFAWSGDLGGQPHCRRGATGYPIFDVLRTQKLDF
                     FLFLGDTIYSDGVCPSPPNEPGADFTASTLEEYRARHRYQRGADSLRRFLESVPVYVV
                     WDDHEVRNNFAGPFERLMPVGRQALRDYWPIRTDPEDPHRLYRTVRCGADLELFILDT
                     RQYRSRNADADGPNKSMLGATQLRWLLEGVRTSTATWKVIATSVPLSIPKGGGEAVPG
                     YDGWAGGPDGTGFERERQVIVDAIIGQKLKNVVFIAGDVHYVQANGYDPNNDGQPDFY
                     EFVVGPLSASPGRLTPPSPALHPTNMINEGGYFNFGLIRVTRSSLEATVIDEAGQTRF
                     THRLTAQ"
     gene            2529..2768
                     /locus_tag="VI359_05615"
     CDS             2529..2768
                     /locus_tag="VI359_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973763.1"
                     /translation="MSELACMIEGCQAKAYAPAGTQNVCKEHFLSFLTWRRRKGPQMF
                     TKYAAMTMEERNTIVTEWQKTVAIVDVPTPSAPKA"
     gene            complement(2833..3402)
                     /locus_tag="VI359_05620"
     CDS             complement(2833..3402)
                     /locus_tag="VI359_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973764.1"
                     /translation="MRDGQAVHSLFGRRALQGAMFVLGLSLVGCLKPVPKVPIPGSDA
                     TPPTLVWEVYNLQTKERREIAQDGQTLDVPSNEQVIVTIAVEDLQSGVKDVSLGGEGQ
                     YACELGGQTDKKAVKLEQQNTTRIPDHENKVPVSASLSYTVEFGKMGCKEAERFAGGK
                     LSLVGKGHNFLNGTGTKSLHFDLKKQASP"
     gene            complement(3427..4353)
                     /locus_tag="VI359_05625"
     CDS             complement(3427..4353)
                     /locus_tag="VI359_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY6973765.1"
                     /translation="MRIVSLLPSATEIICSLGLEDQLVGVTHECDYPPAVLQLPKVTH
                     TLIPKEATSSQIDRLVRQQLKTTRALYALNLLLLKELRPDLIVTQSLCEVCAVAQDDV
                     QAALSQLPGNPRAINLEPQSLLDVFAAMHQVADAAGVSAEGAIQPLTSRIDAVIARSV
                     TLTHRPRVVLLEWLDPPFSCGHWSPELVRLAGGIEGLGQEGRPSRTLSWDEVVTWQPE
                     VIFVACCGLNVERTLQDLSLVQSISGWQELPAVRSGRLYVTDGSQYFSRPGPRLVDSL
                     EMLAHVLHPTVHPLPSGLPAPIAVNRTCVGSK"
     gene            complement(4467..>4653)
                     /locus_tag="VI359_05630"
     CDS             complement(4467..>4653)
                     /locus_tag="VI359_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973766.1"
                     /translation="YEEAIGCPTSGDCYVPGSEHLLSIELLFFYAALIIWPVAAYNLW
                     RQVRSILWTRVIRGGDG"
BASE COUNT         1057 a         1448 c         1219 g          929 t
ORIGIN      
        1 gtcagcgttc cggattccgc acagcagcca ccgcgcgtgc gaacgccagc aggcttttcc
       61 gttcctcgtc atcgagtgcc aacaagagat gcgtctcttc ggcatgcatg agccgtaggc
      121 tgaggaatgg ctcctcacgg cgcttttcgc ggttgtccca catggcgtcg atgacctgga
      181 ccttatccag ctggccgacc gacacgatgt cgtaccgaaa gtacgaatcg ccgatcacca
      241 tatcgaacac gacgttgccg gccgtcaaca gcatcagatt gaatcgcccc tgctcctcgt
      301 acccttcagg tagaaatttg ttgatgtggc acagcgccac cttgcggtca ccgagcgcgg
      361 cgcgaatctt ttctttgagc gcctgataat cgatctgcag tgccttctca tccggtcccg
      421 tcgcggccac cgccgcatct tctgctttct tgaaaatctc atctgccgac atcaaccctg
      481 ccataacgtg ttccccctat ttcgtcgtac agcacgacat gttttgcgag acgttttccg
      541 aagcgcttcg cgccttgatc gcccggacca ctccgactat gccgagcccc gcccaggcaa
      601 actccaaaag aatgaatccc atgcgttgat ctgcaatcgc gacgatcccg agcagcaacg
      661 aaccgacgat gttgaatgcg agatagcctc tgtcgagctc acgccacaca ccgctctgaa
      721 tcaggccgta ggcaacaagc accatgagcg cacccatcac agacacaatc tgtaagacca
      781 tatgcacgac tacccccatt aggcggatcc ggaagtgagc gagtacggta cccgccgacc
      841 agccaagatt gcaagaggat agtcacgaaa gtggcggctt attcgcagac ggtcaaacag
      901 agcatcgtgc tccgatcagg cctgctactg tgccgtcagg cggtgagtaa atcgagtttg
      961 accggcctca tcgatgaccg ttgcttccag tgacgaacgt gttacacgga tgagtccgaa
     1021 gttaaagtag ccgccttcgt tgatcatgtt ggtaggatgc aaagcgggac ttggtggagt
     1081 caggcggccc ggacttgccg agagtggacc aacgacgaat tcatagaagt ccggttgacc
     1141 atcgttgttg ggatcgtacc cattcgcctg tacatagtgc acatcgcccg cgatgaagac
     1201 cacattcttc aatttctggc cgatgatggc atccacgatt acctgccgtt ctcgctcaaa
     1261 acctgtgccg tccggtcccc cggcccaccc gtcatatccc ggtacagcct cgccgcctcc
     1321 tttgggaatt gagaggggga cgctggtggc gatcaccttc cacgtcgcgg tggaggtgcg
     1381 caccccctcg agcaaccaac ggagttgcgt cgccccgagc atggatttgt tcggaccatc
     1441 agcatcggca ttgcgactgc gatattgcct cgtgtccaga atgaacagtt caagatcagc
     1501 cccacatcgt accgtccgat acagtcgatg ggggtcctcc ggatcggtgc gaatcggcca
     1561 atagtcccgc aacgcctgcc gtccaaccgg catcaggcgt tcgaacggac cggcaaaatt
     1621 gtttcgcact tcatggtcgt cccacaccac atacaccggc accgactcga gaaagcggcg
     1681 caacgagtcg gcgcctcgct gatagcgatg acgcgctcgg tattcctcca atgtcgacgc
     1741 tgtgaaatca gcgcccggct cattcggtgg tgaagggcac accccgtcgc tgtagatcgt
     1801 atccccgaga aagagaaaga agtcgagttt ctgtgtccgc agcacatcaa agatcggata
     1861 ccccgtagcc ccacgacggc aatggggctg tcctccgagg tcgccgctcc acgcgaacgt
     1921 caccggcgcc gaagtcttcg cgtcagggag cgtcgtaaat tcacccttcg cctcgacccg
     1981 tgcttcgata tgttgtccct catctccctt tggacccacg aggatatggt agcgataggc
     2041 agtagccggc ataagcccta agaagggaat cgtgagcgta taatccgtct ccgggccagt
     2101 cataaataga ggagttctcg ccacgtgagc tatggctgtg gcctgcttcg atacggcctc
     2161 ccaagccgcg accgacgccc actccacttg aaccctcatc gggccatcag tccgaagcca
     2221 gagcagcgcc gactggctcg tgacatcgcc gatagcaatc ccctgcggca acgatttcga
     2281 ggtggcagcg gaaggcatgg caaaaggact ccccggagga agaccatcgc gagacggcga
     2341 ggtgcaactg aggacagaaa gaaccacgac cggcagcgac gatacgcgac agagctgaga
     2401 cataatgaat gaggacttac cggaacaagg tcggatcgtc aagagaggcc ttgcaatcga
     2461 tgtacgaggc tggttgaaaa tcttatcatc cgtcctgcat actactgctc gaccgagtaa
     2521 ggagaccgat gagcgaactg gcctgcatga tcgaaggttg ccaagcgaag gcgtacgcgc
     2581 cggcaggcac acagaacgtg tgcaaagagc acttcctcag ttttctgacg tggcgacgcc
     2641 gcaaaggtcc gcagatgttc accaagtatg ctgccatgac gatggaggaa cgtaatacca
     2701 ttgtgaccga gtggcagaaa actgtggcga tcgtggacgt gccgactccc tccgctccga
     2761 aggcttaatc gttcgccttc tcccacgcac aagattcctg ccgcatcgtt gagcggcgtt
     2821 ctatcaaccg actcatggcg atgcctgctt cttgagatcg aagtgcagcg atttggtccc
     2881 agtgccgttc aaaaaattat gccccttccc gacgagagac agtttcccgc cggcaaagcg
     2941 ctcggcttct ttgcacccca ttttcccgaa ttccacggtg tacgacagtg aagcggacac
     3001 cggcaccttg ttttcatgat caggaatgcg cgtggtgttc tgctgttcga gtttgacggc
     3061 cttcttgtcg gtctggccgc cgagttcgca tgcatactga ccctccccgc caaggctgac
     3121 gtctttcaca ccgctctgga gatcctccac cgcaatcgtt acgatcactt gctcgttcga
     3181 cggcacgtca agcgtctgcc cgtcttgcgc aatctctcgg cgctccttcg tctgaaggtt
     3241 gtagacctcc cagacaaggg ttgggggcgt ggcatcggaa cctggaattg ggaccttcgg
     3301 aacgggcttc agacacccca ccaaggagag accaaggacg aacatggctc cttgcagtgc
     3361 gcgtcgccca aacaaggaat gtactgcttg accatctctc atcacaccct ccttctctac
     3421 catgatttat ttggagccaa cacacgtcct attgacggcg attggagcag gcagcccgct
     3481 cggcaacgga tgcacggtag gatgaagaac gtgcgccagc atttcaagac tatcgacgag
     3541 gcgaggcccg ggacggctga aatactgcga gccatccgtg acgtacaggc gaccggaccg
     3601 aacggcgggc agttcctgcc agccagaaat cgattgaacc agtgaaaggt cttgcagcgt
     3661 ccgctccacg ttcaagccgc aacaggcgac gaatatcacc tccggctgcc aagtcaccac
     3721 ctcgtcccag ctgagtgttc gcgaaggacg tccttcctga ccaagtcctt caatcccgcc
     3781 agccagccgc accaattcag ggctccagtg accgcacgaa aacggtgggt cgagccattc
     3841 cagcaacacc acgcgcggac gatgagtcag cgtgactgac cgtgcgatca ccgcatcaat
     3901 ccgggacgtg agcggctgaa tcgcgccttc tgcagaaacg ccagctgcat cggcaacctg
     3961 atgcatcgcc gcaaaaacat ccaacagcga ttgcggttcg agattgatcg cgcgggggtt
     4021 gcctggtaat tgcgagaggg ccgcctgcac atcatcctga gccaccgcac aaacctcaca
     4081 gagcgattgc gtcacaatca aatcaggccg caattccttc agcaacagca gattgagtgc
     4141 atacagagcc cgcgtcgtct ttaactgctg gcgcaccaat cgatcgatct ggctactcgt
     4201 cgcttctttc ggaatcagcg tgtgcgtgac cttgggcagt tgcagcaccg caggcggata
     4261 atcgcactca tgggtcactc ccaccagctg gtcttccaac cccagtgaac aaatgatctc
     4321 cgtcgcactc ggcagcagcg acacaattct catcgcaccc ctctaccttg attcaacaaa
     4381 cccccgacct ccaccgcatg acacccagtt cgaaactgtt cctcacaccg tttcctatca
     4441 ctcattgctt catatagaga acagtcctaa ccatctcctc cacgaataac ccgagtccag
     4501 agaatgctcc tgacctgccg ccacaagttg tacgcagcga caggccatat aatcaaggcg
     4561 gcatagaaaa acagcagctc tatcgacagc aggtgttccg agcctgggac gtagcaatca
     4621 ccggaagtcg ggcagccaat tgcctcctcg tat
//
LOCUS       DATDNW010000228         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_987218, whole genome shotgun sequence.
ACCESSION   DATDNW010000228 DATDNW010000000
VERSION     DATDNW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987218"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VI359_05635"
     CDS             <1..802
                     /locus_tag="VI359_05635"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY6973767.1"
                     /translation="TGHLILAGHALGFDGEIAASAEISIQLGAILAVIAYERRKLASM
                     FGQALREQQDVSAMFRGRGTTSWSALIQKSWAIHRNLWFILGLGLAFLPAAAIGFLTH
                     NWIKSHLFTAQTVAISSIIGGLIILAVEARHNQARVLQLEQVSLASAFWIGVAQCASL
                     IPGTSRSGSTIIGGLLVGLDRKIATEYSFFLALPTLIVATCYQMWKSRDVFRQEDYLA
                     LAVGMVVSFIVAWVVIAAFLSFVKHHTLRPFAYYRIVMGLVVFYVFGF"
     gene            818..1087
                     /locus_tag="VI359_05640"
     CDS             818..1087
                     /locus_tag="VI359_05640"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HEY6973768.1"
                     /translation="MAGTQSDTVHVYDTWVKGKKGLLHFDVMTSDETTALTLAKQYLA
                     SIGEADVPVMLKECQFCHSEPLVMFSPEQQRQFREQGGFIVRLSA"
     gene            complement(1084..1617)
                     /gene="lspA"
                     /locus_tag="VI359_05645"
     CDS             complement(1084..1617)
                     /gene="lspA"
                     /locus_tag="VI359_05645"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY6973769.1"
                     /translation="MTKPVLRYFLLALVAGTVIIIDQLTKLSIMQNMRLHESIPIIPN
                     LFSLTYIRNPGAAFGLLAGSSNAFRTLFFGVTSLFALGLLGTILVRLPANDWMGQLSI
                     ASILGGAIGNLLDRLQYGEVIDFLDFYVDAYHWPAFNVADSAISVGVVFLIIHFAFEK
                     KEAPAVPSGSASSSASH"
     gene            complement(1614..>4317)
                     /gene="ileS"
                     /locus_tag="VI359_05650"
     CDS             complement(1614..>4317)
                     /gene="ileS"
                     /locus_tag="VI359_05650"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY6973770.1"
                     /translation="LYERIQEAGKGRERYLLHDGPPYANGRIHIGHALNKILKDIIVK
                     SKTMAGYHVPYVPGWDCHGLPIEHQVLKDLGEKKKTLDAMAIRRLCRDYAEKFYKIQR
                     EEFQRLGVLGDWQHPYLTMNPAYEAAIIRTFGQFVERGGVYKGLKPVLWCTQDQTALA
                     EAEVEYEDHTSPSIYVKFPVAGSPASFSSRFPGLTIPASVRSMSIVIWTTTPWTLPAN
                     QAVCLHADIAYAFAQVGNEVLIVAHKLLDSVAKACKLENVKLLAVKKGCEGFEGLETQ
                     RPLTTGLSPVLLGDFVTLDQGTGCVHIAPGHGMEDYLLVLEYNATASPGERLEILAPV
                     DDAGRFTEVVTEFAGQHVFRANSGIVERLKQNGRLLGHDSLSHSYPHCWRCKSPVIFR
                     ATEQWFVSMDINDLRKAALAEIDRVQWIPPWGKDRINGMIANRPDWCLSRQRVWGVPI
                     PGFTCTACRHVLADVRVIEHIATLVETNGADVWFERSAADLLPAGTTCPKCGKTDFEK
                     ERDILDVWFESGVSYAAVLKPCKWWPADLYLEGSDQHRGWFHSALLAGVVTDRRAPYQ
                     AVLTHGFVLDGQGKKMSKSAGNVVAPQDVIKQSGAEILRLWVAAQDYRDDLRISPEIL
                     NHLIEAYRKIRNTCRFLLSNLYDFDLTTHRVAYEQLPELDRWALTRLAELVPRVRQSY
                     EAFEFHTIFHALNNFCSVDLSAVYLDILKDRLYTFRADSPLRRGSQTVFFEIVTVLTK
                     LMAPVLSFTAEEIWRALHPQQAGHEASIHLSSFPDADRKWADGQLALRWEKLLSVRER
                     VQGVLETSRREKLIGSSLEAHVTLLADAKTYEFLKPMASDLSTVFIVSQVTLTQQSGA
                     AQDLVVTVTKSPFAKCERCWNYREAVGKDATHPTLCDRCVEAVR"
BASE COUNT          942 a         1240 c         1183 g          952 t
ORIGIN      
        1 caccggccat ctcatcctcg ccggacatgc gttagggttc gacggcgaga tcgccgccag
       61 tgccgagatt tcgatccagc tgggcgccat tcttgccgtc atcgcctacg aacgccgaaa
      121 acttgcctcc atgttcggac aggcgttgag ggagcagcag gatgtctccg cgatgtttcg
      181 tggtcgtgga acgacttcat ggtcggcttt gatacagaaa tcttgggcga ttcaccgcaa
      241 tctctggttc attctcggcc tcggcctcgc cttcctgccg gcggcagcaa ttggatttct
      301 tacccacaat tggatcaaga gtcatttgtt taccgctcaa accgtggcga tctcctcgat
      361 tatcggtggg ctcatcattc tggctgtcga agcccgccac aaccaggctc gggtcctcca
      421 gcttgagcag gtgagtttgg cctcggcctt ctggattggc gtggcccaat gcgcctcttt
      481 gatcccgggc acgtcccgat ccggctcgac gattattgga gggctactgg tcggacttga
      541 tcgcaagatc gcgacggaat attcgttctt cctagcccta cccacgctga tcgttgcgac
      601 ttgctatcaa atgtggaaat cgagggatgt attccggcag gaggactatc tggcgctcgc
      661 cgtcgggatg gtagtctcgt tcattgttgc gtgggtcgtc attgcggcat ttctctcctt
      721 cgtcaaacac catacgcttc gaccgttcgc gtattatcgc attgtgatgg gtctcgtggt
      781 gttctatgtt ttcggattct agaggagacg agtgtcgatg gcaggcacac agtccgatac
      841 cgttcacgtg tacgacacgt gggtgaaggg gaagaaggga ctcttacatt tcgatgtcat
      901 gacctctgac gagacgacgg ccttgacact ggccaaacaa taccttgcaa gcattggaga
      961 ggcggacgtc ccggtgatgc tcaaggaatg ccagttctgc cacagcgagc cactcgtgat
     1021 gttttcgccc gagcagcaac gtcaattccg agagcagggc ggttttatcg tgagattgtc
     1081 ggcctaatga ctggccgatg acgacgcgct tccggacgga accgccggtg cctctttctt
     1141 ttcaaatgcg aagtggatga tgaggaagac gacgcccaca ctgatcgccg agtcggcgac
     1201 attgaaggcc ggccagtgat aggcgtcgac gtaaaaatcg agaaagtcga tcacctcgcc
     1261 gtattgcagc cggtccagca gatttccgat cgcgccaccc aagatcgatg cgatgcttaa
     1321 ttgtcccatc caatcgttcg cggggagccg gaccagaatc gtccccaaca atccgagggc
     1381 aaacaacgat gtgaccccaa agaacagggt acgaaacgca ttgctgctcc cggccaataa
     1441 gccaaacgcg gccccaggat tcctgatata cgtgagactg aacagattgg gaatgatggg
     1501 aatcgattca tgcaaccgca tgttctgcat gatcgaaagc ttggtcagtt gatcgatgat
     1561 gatgaccgtg ccggcaacga gcgcaagaag gaaatagcgg aggaccggct ttgtcatcga
     1621 acggcctcca cacaacgatc gcagagcgtg gggtgggtcg catccttgcc gacggcttcg
     1681 cgatagttcc agcaacgttc gcacttggcg aatggagact ttgtcactgt gacgaccaaa
     1741 tcttgtgccg ctccggactg ctgagtcaat gtaacctgtg acacaataaa gaccgtgctc
     1801 aaatccgagg ccatgggttt gagaaactcg taggtctttg catctgcgag caacgtcaca
     1861 tgagcctcca gcgacgagcc aatcaacttt tcccgtcgac ttgtttcgag aacaccctgc
     1921 acacgttccc ggaccgataa aagcttctcc cagcgtagtg ctaactgacc atcggcccat
     1981 ttacgatctg catcaggaaa agaggaaaga tgaatgcttg cctcgtggcc tgcctgttgg
     2041 ggatgtaacg cgcgccaaat ttcctctgcc gtaaagctca acacgggtgc catcagtttc
     2101 gtcaaaaccg tgacaatctc aaaaaacacg gtctgggacc cgcggcgcaa cggcgaatct
     2161 gcgcggaagg tatagagtcg gtctttcaag atgtccagat agacggcgct caggtcgacc
     2221 gagcagaagt tattcaatgc gtggaaaatc gtatggaact cgaaagcctc atacgactgg
     2281 cggaccctcg ggactaactc agccagtcgc gtcagcgccc accggtccag ttcaggcagt
     2341 tgttcatagg ccactcgatg ggtcgtcagg tcaaaatcgt acaaattgct tagcaagaac
     2401 cggcaggtat tgcgaatctt gcgataggct tcgatgaggt gattgagaat ctccggtgag
     2461 atccggaggt catcgcgata atcctgagcc gcaacccaca gtcgtaagat ctctgccccg
     2521 gattgcttga tgacatcctg gggcgccacc acgttccctg ccgacttcga catctttttc
     2581 ccctgtccat cgaggacaaa cccatgggtc aagactgctt gatagggagc tcgtcgatcc
     2641 gtcaccactc cggccagcaa cgcactatga aaccatccgc gatgctggtc ggacccttcg
     2701 agatacagat cggccggcca ccacttgcat ggcttgagca ctgcggcata gctcacgccc
     2761 gattcgaacc acacgtcaag gatgtcccgt tccttttcaa aatctgtctt cccacatttc
     2821 gggcacgtgg tgccagcagg caagaggtct gccgccgatc gttcgaacca tacgtcggcg
     2881 ccgttcgtct ccaccaatgt tgcgatgtgt tcaatgaccc gaacatcggc gagcacatgg
     2941 cgacaggccg tacaggtgaa tccggggatc ggcacgcccc acacacgctg acgtgacaag
     3001 caccaatcag gtcggttggc aatcatgccg ttgatgcgat ccttacccca tggtgggatc
     3061 cattgcacgc gatcgatttc agcgagcgcg gccttccgaa gatcgttgat gtccatcgag
     3121 acgaaccatt gttcagtcgc gcgaaagatg accgggctct tgcaccgcca acagtgggga
     3181 tacgaatggc tcagcgagtc gtggccgaga agccgtccgt tttgtttcaa ccgttccacg
     3241 atgccggaat tcgccctgaa cacgtgttgc ccggcaaact cggtgaccac ttccgtaaac
     3301 cgccccgcat catccaccgg cgcgagaatc tccagccgtt ctcctggtga ggccgtggcg
     3361 ttgtactcca acaccaggag atagtcttcc ataccgtgac ccggcgcgat atgcacacag
     3421 ccggtccctt gatccagcgt cacaaaatcc ccgagcaaca cgggcgacag gccggtggtc
     3481 aatggacgct gcgtctccag accttcgaac ccctcacaac cttttttcac cgcgagtagc
     3541 ttcacgtttt ccaacttgca ggccttggcc acgctatcaa ggagcttgtg agccacaatc
     3601 aggacttcgt tcccgacctg ggcgaacgca taggcgatat ccgcgtgaag gcagacggcc
     3661 tggttggccg ggagtgtcca gggcgtcgtc gtccagatca cgatcgacat tgaacggacc
     3721 gaggcaggaa tcgtgagccc aggaaatctt gagctgaatg acgcaggtga cccggccact
     3781 gggaacttca catagatcga tggcgaggtg tgatcttcat attcgacttc ggcctcggcc
     3841 agagccgtct gatcctgcgt gcaccacagc accggcttga gtcctttgta gacgccccct
     3901 cgttcgacga attgcccgaa cgtgcgaatg atcgccgcct cgtaggccgg attcattgtg
     3961 agatagggat gctgccagtc gcccaggact ccgagccgct gaaattcctc gcgctgaatt
     4021 ttatagaact tctccgcata gtcgcggcag agacggcgga tcgccatggc atcgagcgtt
     4081 ttcttcttct caccgagatc tttcaggacc tggtgttcta ttggcaaccc gtggcaatcc
     4141 catcccggca catagggaac gtgatagccg gccatagtct tggatttcac aatgatgtct
     4201 ttgaggattt tgttcaaggc atggccgatg tggatacgcc cgttggcata gggtggaccg
     4261 tcgtggagca gatagcgctc ccgtcccttg cccgcttcct ggatccgctc atagagt
//
LOCUS       DATDNW010000229         4836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_371836, whole genome shotgun sequence.
ACCESSION   DATDNW010000229 DATDNW010000000
VERSION     DATDNW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371836"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VI359_05655"
     CDS             complement(<1..680)
                     /locus_tag="VI359_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY6973771.1"
                     /translation="MFVILGATGDLTRRKLLPALFHLRDPGSIEKPTRLIVGAALPEM
                     SEEAFRLWAYEGLHNAGSRNANELRAWCDACLHYQSVHEGGPQDYEALAGFIRQLERT
                     SHIPENRVFYLALPPASVPLAIEQLGQAGLLKARGWVRVVFEKPFGHDFHSARALNTE
                     LHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWKRDSVESVQITVAEDLGVE
                     HRGAYYQQA"
     gene            complement(1035..1274)
                     /locus_tag="VI359_05660"
     CDS             complement(1035..1274)
                     /locus_tag="VI359_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973772.1"
                     /translation="METTNVNVPDRAAAWVERQLTLAVYTAIVGLACVLLFLWYGFGA
                     WTMGLGTFIGIPLLGLAFLLYLSAVVADLKRRRAF"
     gene            complement(1246..2022)
                     /locus_tag="VI359_05665"
     CDS             complement(1246..2022)
                     /locus_tag="VI359_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY6973773.1"
                     /translation="MTEQRPRRRPMLFLMAFFVEAWLLASPAWAVETHGRWSDVDVLR
                     AMGIAAAVAGILLLILVQHVYRNKIQHSTYRRLLLIGLFVLPMIVTWSTTRTVMEGTK
                     SVQACMSCHVMHPFVNDMENPTSRTLAARHYRNNWIAKDQCYSCHVTYGATGTVEGKR
                     DGFRHWLYYITNTYADPIQYAGSYDNSNCMNCHRGTGKWERVKSHQALVAELAADRVA
                     CISCHGPPHPLPSERKKMASRSTMTAELGGHYGNDERQRP"
     gene            complement(2038..2253)
                     /locus_tag="VI359_05670"
     CDS             complement(2038..2253)
                     /locus_tag="VI359_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY6973774.1"
                     /translation="MKDGDPVVVKSRRGEVELRARLTEVVQPGMVFATMHSARHLVNQ
                     STHDVYDPFSKQPAYKRCAVAVRRKMA"
     gene            complement(2263..4314)
                     /locus_tag="VI359_05675"
     CDS             complement(2263..4314)
                     /locus_tag="VI359_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973775.1"
                     /translation="MPEQSNEPTGLTRRELTKLMGASVGLACLSPLAGCGELWERQPV
                     IPVDTWHKGVCRFCGTGCGVMIGLKGNKVADVKGDDEAHNRGRLCIKGLATRDILYSE
                     GRALYPMIRKDGELQRASWDDAMTLVAQRFKESIETHGPDSVAYYGSGQLYTQESYTA
                     NKLFKAGIGTNNVDGNPRLCMASAVTGYTTTFGKDEPAGCYEDIDDAECFFITGSNTL
                     ECHPIIWERVRDRKRSHPNTRIILVDPRTTFTARHADLHLPICPGTDVSLYNAMIHVF
                     IEQKFIDADMVQQYLSFQEGEEKRTFDSFKRHIAKYTPEAAARTCGITARDIREAAFL
                     FASSKASMSLWTMGLNQQAQGTASNRLVCAMHLLTGHFGRPGATPFSLTGQPNACGGV
                     RDTGSLAHALPNGRLVANPKHREEMEDLWKVPRGRISPKPGYHAVALFDAMNRGAVNC
                     CLVMCTNPGQTLPSVTTYRQGMEKAFLVVVDAFHPTETTKYADVVLPAALWVEKEGVT
                     GNAERRYHLYPKLVDPPGEAKSDLDILVDLADRLGHGNLITARTPREVWDEWRKVSAH
                     SKYNFEGITYERLEKERGLLWPCPTETHPGTKRRYVPGEDPMATGTGRFDFYGKPDGR
                     ATVWLDHQEEPLDPYTAEFPLIFNTGRILEHWHTMTITGPVPTLQGIHPDYLEIHPYD
                     A"
     gene            complement(4568..>4836)
                     /locus_tag="VI359_05680"
     CDS             complement(4568..>4836)
                     /locus_tag="VI359_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973776.1"
                     /translation="LPVVGSDRCSPLYGVAQFELVDETGSLPVETLGSCLAASADLPH
                     DGDVIELTAEIQVFVPEGQRQRVIKAITQKIAVLRVAPDGARAP"
BASE COUNT          958 a         1454 c         1426 g          998 t
ORIGIN      
        1 gcctgttggt agtaggcacc gcgatgttcg acacccagat cctcggccac ggtgatctga
       61 acgctctcga cgctgtcgcg cttccagagt gattcaaaaa tcggattggc aaaccggaac
      121 gccaggagat tctgcaccgt ctctttgccc aggtagtggt cgatccgata gatctgggac
      181 tcgtcgagat agcggtgcag ctcggtgttt aaggcccggg ccgagtgaaa gtcgtggccg
      241 aacggttttt cgaacacgac acggacccac ccccgcgcct tgaggaggcc tgcctgcccc
      301 aattgctcaa tggcgagagg cacagaggcc ggcggcaggg ccagataaaa cacacgattc
      361 tcgggtatgt ggctggttcg ctccaattgt cggatgaacc cggccaacgc ctcgtaatcc
      421 tgcgggcccc cttcatgcac cgactggtaa tggagacagg cgtcgcacca ggcgcgaagt
      481 tcgttggcgt tgcgcgaccc cgcattgtgc agcccttcgt aggcccagag gcgaaaggcc
      541 tcttcgctca tctccggcag cgcagctccg acaatcagcc gggtcggttt ctctatgctc
      601 cccggatcgc gtaaatgaaa gagcgcgggc aggagcttcc gccgggtcaa atcgcccgtc
      661 gcgcccagaa tgacaaacac atgtggctca ccgatgggtt cctgcactcg cgtcgatccc
      721 ctttgccaca gtgtaccagt tcctgaatga ttgagggtct tccttcgtgc ttctcaccac
      781 ctgcggccgg cgagcacagt ctttcacaga ccgcgcgcgg acgaggcgtt gcaacaaaga
      841 ctccctaccc tttttcttaa ccgtcttttt tgtccctcga aaagctactg cgaggagaga
      901 agtctgtcaa cggagggaga accggtcagc ttccgcgcgc gggcgaggcg aaatggttcc
      961 tgcacctttg tcttccgtgg ccagatacta acttgagaag acgcggggac acttaactga
     1021 aagctgcccc ccacttagaa ggcacgccgc cttttcaggt cggcgacgac ggcactgagg
     1081 tacagcagaa aagcgagacc gagcagaggg atgccgatga aggtccccaa ccccattgtc
     1141 cacgcgccga acccgtacca aagaaaaagc aacacgcagg cgaggcccac aatggcggta
     1201 tacacggcca gcgtcaattg cctctcgacc catgccgcgg ctcgatcagg gacgttgacg
     1261 ttcgtcgttt ccataatgac ctcccagctc cgcggtcatt gttgatcggc tcgccatctt
     1321 ttttcgctcg ctcggtaggg gatggggcgg tccatgacag gagatacaag cgacgcggtc
     1381 agctgccagt tctgccacga gcgcctggtg gctcttcaca cgctcccact tgccggttcc
     1441 ccgatggcag ttcatacaat tggaattgtc gtaactgccc gcgtactgaa tgggatccgc
     1501 ataggtattg gtgatgtaat acagccagtg ccggaatccg tctcgtttac cttccacggt
     1561 gccggtcgct ccgtaggtca cgtgacagga gtagcactgg tcctttgcaa tccagttgtt
     1621 ccggtagtgc cgtgcggcca acgtccggct ggttggattt tccatgtcgt tgacgaaggg
     1681 atgcatcacg tggcaggaca tgcaggcctg caccgacttg gtaccttcca tgaccgtcct
     1741 cgtggtactc caggtcacga tcattggcag cacaaacaac ccgatgagga gcagacgccg
     1801 ataggtacta tgttgaatct tgtttcggta cacgtgctga accagaatga gaagaaggat
     1861 gcctgccact gccgccgcta tgcccatcgc gcgcaagaca tcaacgtccg accatctgcc
     1921 gtgcgtctcg accgcccatg caggagacgc caggagccac gcttccacaa agaacgccat
     1981 gagaaaaagc atcggacgcc gacgaggccg ttgttctgtc atggtcagag tcctccgtta
     2041 ggccatcttc cgccgaaccg cgaccgcaca acgtttgtag gccggttgtt tcgaaaaagg
     2101 atcgtagacg tcatgggtgg attggttcac cagatgcctg gcggagtgca tggtcgcgaa
     2161 taccatacct ggctgcacaa cctccgtcag acgggcccgc agctcgactt ctccgcgccg
     2221 gctcttcacc acgaccggat cgccatcttt gatctgtaac tgctaggcat cgtaggggtg
     2281 gatctccagg tagtcggggt ggatcccttg gagtgtgggt accggaccgg tgatggtcat
     2341 cgtatgccag tgctccagaa tccggcccgt gttgaagatc agcggaaact cggcggtgta
     2401 gggatcgagc ggttcttcct gatgatctag ccagacggtg gctcgcccgt ccggttttcc
     2461 gtagaaatcg aaccggccgg tgcccgttgc catggggtcc tctcccggaa cgtaccgccg
     2521 cttcgtaccc ggatgcgtct ctgtcgggca gggccagagg agcccgcgtt ctttctccag
     2581 tcgctcgtag gtgatgcctt caaagttgta tttcgaatgg gccgagacct tgcgccactc
     2641 gtcccagacc tccctcgggg ttctggccgt gatcagattc ccgtggccga gccgatccgc
     2701 cagatcgacg agaatatcca agtcgctttt ggcctcgccc ggtggatcga cgagcttcgg
     2761 gtagagatga tagcgccgct ccgcgttgcc cgtaacgcct tctttctcca cccacagagc
     2821 agcgggcagc accacgtcgg catatttcgt ggtctccgtc ggatgaaacg cgtccaccac
     2881 aaccaggaag gccttctcca tcccttgccg gtaggtcgtg acgctgggga gcgtttgccc
     2941 cgggttcgtg cacatcacca gacagcagtt cactgcgccg cggttcatcg cgtcaaacag
     3001 ggcgacggcg tggtagcccg gcttcggact gatccgtccg cgcgggactt tccacaaatc
     3061 ctccatttcc tctcggtgct tggggttggc cacgagccgt ccgttcggca gcgcatgcgc
     3121 gagagaaccg gtgtcgcgca ccccgccgca ggcgttcggc tgaccagtca acgaaaacgg
     3181 cgtggcgccg gggcggccaa agtgtcccgt caggagatgc atcgcacaga ccagccggtt
     3241 ggacgcagtc ccctgcgctt gctggttcag ccccatggtc cacaacgaca tcgaggcctt
     3301 cgatgaggcg aacaggaacg ccgcttcgcg gatgtcccgt gcggtgatgc cgcaggttct
     3361 ggcagccgcc tccggcgtgt atttcgcaat gtgccgcttg aagctgtcga acgtccgttt
     3421 ctcttcgcct tcctgaaacg agaggtactg ctggaccatg tccgcgtcga tgaacttctg
     3481 ttcgatgaac acgtgtatca tcgcgttata gagggagacg tcggtgccgg gacagatcgg
     3541 caggtgcagg tccgcatgcc gtgccgtaaa ggttgtgcgt ggatcgacga ggatgatcct
     3601 ggtgttggga tggctccgtt tgcgatcgcg cacacgttcc caaataatcg gatggcactc
     3661 cagggtgttt gatcccgtga tgaaaaagca ctcggcatcg tcgatgtctt cgtagcagcc
     3721 agctggttca tccttgccaa aggtcgtcgt gtaaccggtg acagccgacg ccatgcacaa
     3781 gcgcgggttg ccgtcgacat tattggtccc gatgccggcc ttgaacaatt tattcgcggt
     3841 gtagctttcc tgagtgtaca gctgcccgct accatagtag gcgacgctat ccggcccatg
     3901 tgtctcaatt gattccttga accgttgcgc gaccagcgtc atcgcgtcgt cccagctcgc
     3961 ccgctggagt tcgccgtctt tccggatcat cggataaagg gcacggcctt cggagtagag
     4021 aatgtcgcgg gtagcgagac ccttgataca gaggcggcca cggttgtgcg cctcgtcgtc
     4081 tcctttgacg tcggcaactt tgttcccctt gaggccgatc atgacgccgc agcccgttcc
     4141 gcagaagcgg cagacgccct tgtgccaggt gtctacgggg atgaccggtt ggcgttccca
     4201 gagctcgccg cagccggcga ggggcgaaag acaagccagt ccgacggagg cgcccatgag
     4261 cttggtgagc tcccggcgtg tcagtccagt tggctcgttc gattgttccg gcatggcagt
     4321 accctccaca tccaattaat gagagtcctt cgggacggat agtcgttcgc gaggatagag
     4381 aagctctcgg agcgagtgac ccaccactcc gagacaacgc cccaggaaat cgtgcgtacg
     4441 ctagaacaag gcaccccaaa aatcaatgag gaaaccattc aactgaggag ctggagaaat
     4501 gcgtgtgttc cagccgccgc gtgcggacga ggcgatgaga agaattgggt aatcccgttc
     4561 gctctggcta aggtgcacga gctccatctg gggcaaccct gaggacggcg atcttttgag
     4621 tgatggcctt gatcacccgc tgtcgttgcc cttcaggtac aaagacctga atctcggccg
     4681 tcagttcaat cacatcacca tcgtgaggaa ggtcggcaga tgcagcgagg caactcccca
     4741 gcgtctcgac tggaagagaa ccggtctcgt caaccagctc aaattgagcg accccgtaca
     4801 aggggctaca gcggtcagac ccaaccacgg gcaagg
//
LOCUS       DATDNW010000230         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_503768, whole genome shotgun sequence.
ACCESSION   DATDNW010000230 DATDNW010000000
VERSION     DATDNW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503768"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..459)
                     /locus_tag="VI359_05685"
     CDS             complement(34..459)
                     /locus_tag="VI359_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973777.1"
                     /translation="MDVEIGSNLYRNTNGMIAIEGVPQIEVAVKPATGTLLVNFALFD
                     GAGKMIAKLSDSTLMFNERRAYEVSKTAKTLALKHIDSGKIILQMELRAPEVVAITKG
                     EFHTVKGHVLEVTPTEWKIDKVRSSGTKHDMQGGEVAIG"
     gene            complement(503..718)
                     /locus_tag="VI359_05690"
     CDS             complement(503..718)
                     /locus_tag="VI359_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973778.1"
                     /translation="MVGTIKVFLWWFVVGAAMALAVIMLQGGIREVMQAQGSVWELKL
                     VELLTTIMGGGLLGGCIALILNRIKKS"
     gene            complement(728..2257)
                     /locus_tag="VI359_05695"
     CDS             complement(728..2257)
                     /locus_tag="VI359_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY6973779.1"
                     /translation="MLAKVLSAALVGLDACLIDVEVDIAGGLPQFSVVGLPDATVRES
                     RDRVRSALKNTGFHFPAKRITVNLAPAGIKKEGSALDLAIAIGILVAEEVIPPESVMR
                     RVFVGELSLDGHIKRIPGALSIGIACRPNYSLLLPAENSQEAAMVENVEVYPIHTLPE
                     AVAFVKGMIMIEPMRGDGDNLFRARPMEEEDYGDVKGQEHAKRALEVAAAGGHNILMV
                     GPPGSGKTMLAKRLPTILPLMESDEAIETTRVHSVAGQLPADRALLTVRPFRAPHHSI
                     SDAGLIGGGTIPRPGEVSLAHNGILFLDESPEFRRPVLDGLRQPLEDGHVVLTRASGS
                     LRYPARFMLVAAMNPCPCGYYGDPARDCICTPQQIRRYRGRLSGPLQDRLDIHIDVPS
                     VAVREFRDNGGTPEHSEAIRARVLAARARQSHRYREEGIHTNAQLKPRQLKRYCGLDG
                     PSHTLLERSMTKLGLSARAHGRILRVARTIADLAGSDTIGPAHLAEAIQYRSLDRPTQ
                     F"
     gene            2578..>2872
                     /locus_tag="VI359_05700"
     CDS             2578..>2872
                     /locus_tag="VI359_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973780.1"
                     /translation="MEVRTPASHIGAAMALLATLQDGDVLPPEGTPAANRIVKSVIQC
                     QSLFMKSSDPDVRAFFDRALQRKLNGHATETIRRFRNEGWTSEVLEALSESFVA"
BASE COUNT          639 a          839 c          744 g          650 t
ORIGIN      
        1 ccgaccgtga caggaacata agtgttcatc aatctacccg atagcaacct ccccaccctg
       61 catatcatgc tttgtcccgc ttgaccgtac cttgtcaatc ttccactccg tcggcgtgac
      121 ttcaagcacg tgccctttga cggtatggaa ctcgcctttg gtaatggcaa ccacctcagg
      181 ggcacgcaat tccatctgaa gaatgatttt ccccgagtca atatgtttca aggcgagcgt
      241 cttcgctgtt ttgctcactt cgtatgcccg ccgctcgttg aacataagcg tgctgtccga
      301 caacttggcg atcatttttc cggctccgtc gaagagtgca aagtttacca gcaatgttcc
      361 ggttgcaggt ttgacagcca cctcgatttg aggcacccct tcaatggcaa tcataccgtt
      421 ggtgttgcga tataaattcg atccgatttc tacatccata gtgccctttg tccttctggt
      481 gttgtaagga tatgatcgac ggctaggatt tcttgatacg attcaggatc aaggcaatac
      541 atccccccag caggccgccg cccatgatcg tcgtcagcaa ttcgaccaat ttgagttccc
      601 agacggaccc ctgagcctgc atcacttctc tgatgccgcc ttgcagcatg atcacggcca
      661 aggccattgc ggcccccact acaaaccacc agagaaacac tttgatggtg ccaaccatgg
      721 tcgttcatca aaactgagtg ggacggtcga ggctccgata ttgaatcgct tcggccaaat
      781 gcgccggccc aattgtatca gagcccgcta agtcggcaat agtccttgcc acacgaagaa
      841 tacgcccgtg ggcacgagcg gacaggccta gtttcgtcat cgaccgctcc aagagagtgt
      901 gactcggccc atccagccca caataccgtt ttagctgacg aggcttcaac tgggcattcg
      961 tgtgaattcc ctcctcgcga taccgatgcg actgtctcgc acgggccgcg agtacacgag
     1021 cccgaatcgc ctctgagtgt tccggagtac cgccattgtc acggaattca cggaccgcga
     1081 ctgaaggcac atcgatgtgg atatccaggc ggtcctgaag cggaccagaa agccgtcctc
     1141 gataccgacg tatctgttgc ggcgtgcaga tgcaatcccg ggcggggtcg ccatagtacc
     1201 cgcaaggaca aggattcatc gcggcaacca gcatgaaccg cgctggatac cggagcgagc
     1261 cactggctct cgtcaagacg acgtggccgt cttcaagcgg ttgccggagc ccgtctaaga
     1321 ccggccgacg gaactcggga gattcgtcca ggaacaggat gccattatga gccaaggaca
     1381 cctctccagg gcgcggaatc gtacctcccc cgatgagtcc ggcgtccgaa atactgtggt
     1441 ggggagctcg aaacggtcgg acggtgagaa gtgcccggtc ggcaggtaac tgacccgcca
     1501 cgctgtgcac gcgtgtcgtc tcaatggctt catcagattc catcaatggc agtatcgttg
     1561 ggagccgctt cgccagcatg gttttacccg accctggcgg accgaccatc aaaatgttat
     1621 gcccgccggc agccgcaact tccagagccc gctttgcatg ctcctgtcct ttcacgtcgc
     1681 catagtcctc ttcctccata gggcgcgctc gaaagagatt gtcaccgtct ccccgcatcg
     1741 gctcgatcat aatcatgcct ttgacgaacg cgacggcttc aggaagcgtg tggatcggat
     1801 agacttcgac attttcgacc atggccgctt cttggctatt ctccgccggc agaaggagac
     1861 tgtagttggg tcgacacgcg atgccaatcg agagcgcacc aggaatgcgc ttgatgtgtc
     1921 catcgagaga gagctcgccc acgaacactc gtcgcatcac actctccggc gggatgacct
     1981 cttccgccac aagaatgccg atcgcaatgg ccaaatctag ggcggatcct tctttcttga
     2041 ttccggctgg cgcgagattc accgtaattc tttttgcggg gaaatgaaat ccggtattct
     2101 ttaacgcaga tcgcacccga tcgcggcttt cacggactgt tgcgtcggga aggcccacga
     2161 cagaaaattg cggaaggcca ccggcgatat cgacttctac atcgatcaga caggcatcga
     2221 gaccgaccag cgcagcgctc agtacttttg caagcatagc aaacctaata tcacaccgtg
     2281 attcagcgaa gggattataa aaagcacttc caacactttc aactcgcgac gaaggcaatg
     2341 ctggtatttc cgttctgtga tgcagggtaa gaggagccga tagatggtgg aaccgtgact
     2401 ccctctcccg gtataatttc acccgctcga tccgtcatct ccgttctcga caaatttatg
     2461 tttacctttg tctcccctta tcgatccgaa atcatgcgaa ttggatggat ctgcggcctc
     2521 atcgctggcc agagcgtttt attctgtttg atgaatgagg cccacgctga tgtacgaatg
     2581 gaggttcgga ctccggccag ccatatcgga gctgccatgg cgctcttggc gacattgcag
     2641 gacggcgacg tgctcccgcc ggaaggcaca cctgctgcga accgcatcgt gaaatcggtt
     2701 attcaatgtc agtcgctttt catgaaaagt tcggatccgg acgttcgagc attctttgat
     2761 agggcgctgc agcgtaaatt gaatggccac gccacggaga ccattcgacg gttccgaaat
     2821 gaaggctgga cctcagaggt actcgaagca cttagtgagt catttgtagc cc
//
LOCUS       DATDNW010000231         4090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1257003, whole genome shotgun sequence.
ACCESSION   DATDNW010000231 DATDNW010000000
VERSION     DATDNW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257003"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..708)
                     /locus_tag="VI359_05705"
     CDS             complement(193..708)
                     /locus_tag="VI359_05705"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6973781.1"
                     /translation="MTKNIYTGKVITMNVDTVTLPNGVTVDLEIVRHPGASAIVPLKE
                     DGTVVLIRQFRHAAGGFIYEIPAGKLSPGEEPMACAARELEEEIGYRAGSFELLSSIF
                     TAPGFTDEVIRIYKATGLMKGRQQLDRDEVLEVIEMPLYEAMTMIKTGTIRDAKTIVG
                     LQAVYLGSEHR"
     gene            complement(802..1932)
                     /gene="gcvT"
                     /locus_tag="VI359_05710"
     CDS             complement(802..1932)
                     /gene="gcvT"
                     /locus_tag="VI359_05710"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY6973782.1"
                     /translation="MSNSSVCEKAMQRTPLITQHQSAGAKLVDFAGWEMPIQYSGVVD
                     EYQTVRTRVGLFDVSHMGRLWLSGADVVAFLQRVTTNDVGKLTVSQAQYSTVCNEQGG
                     IKDDIFIYRIASEDFLLCVNASNREKILSWLRSHLASTKAVILQDRSTRLSQIAIQGP
                     QSRELLVSLGGTILTTLKLHHACQANLGGFPCLIARTGYTGELGYEIYVDADKVGRVW
                     DLFMQKGSAWGLKPAGLGARDLLRLEMGYLLYGNDINEETTPLEANVEWTVSFQKGDF
                     IGSQALLTQKEAGVPRRFMAFELVEKAVPRHGFKILDSVSSRVIGEVTSGNFSPLLQK
                     GIGLGYVPPSYAAPATALAIDIRGKAVPAVVVKAPFYKKPKG"
     gene            complement(1910..2278)
                     /locus_tag="VI359_05715"
     CDS             complement(1910..2278)
                     /locus_tag="VI359_05715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY6973783.1"
                     /translation="MKGSDKTTGKNEITTSSGLTYRDEVVGTGEVAVAGKTATVHYTG
                     WLENGKQFDSSVDRGQPFSFRLGAGQVIRGWDEGVEGMKVGGKRRLTIPANLGYGARG
                     AGGIIPPHATLIFDVELLGV"
     gene            complement(2372..3580)
                     /locus_tag="VI359_05720"
     CDS             complement(2372..3580)
                     /locus_tag="VI359_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY6973784.1"
                     /translation="MITHSSRATHNSTLQFASALSRSADTEAAARDVADIVRRQIGEN
                     SVDLAFAFFSAHHVAHATVISTMLQEALQPEVCVGCSGEGVIGGSEELETAPAITLWV
                     ARLPQVHMTPLRLSFSDVRDQIQITGWPESTAAESTFILVADPFTTPMRDVLSVMADR
                     YPQAKAIGGLAGGGHDVGANRLLLNHEVFDGGLVGVRLDGPVSVRTVISQGCRPIGDR
                     FVVTKAERNLVYELGGKPALQRLQDVFESLESTHRRDAHLALHLGIVIDEHRNHFERG
                     DFLVRNLIGADQQAGAIAIGDVVEEGQTVQFQLRDGISASEDLQVLLAKDRTIHRTPP
                     LSALLFSCCGRGQGLFGYPHHDSGAIHERFGDIPVAGFFAQGEIGPVGGRNFLHGYTA
                     SVALFAEPDS"
     gene            complement(3602..3829)
                     /locus_tag="VI359_05725"
     CDS             complement(3602..3829)
                     /locus_tag="VI359_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973785.1"
                     /translation="MNEYQIGGGLRLLTAVEKTDAFGEFLKTRMVRALESQDPTELHY
                     LLAQLDDYYSYMWRYYKKLAKDRAERMDPGV"
BASE COUNT          877 a         1156 c         1174 g          883 t
ORIGIN      
        1 ttccttcgcc ggtgaagaaa agaaggacaa gaaggatgag aagaagggcg gccacgtcgt
       61 gatttacggc gaggagaaga aggacgagaa gaagaaggac gagaagaagg gcaagtaatc
      121 cccgtttttg gcgaggctag cgggcttaag gagcctcccg tcagaaacgg gaggctcctt
      181 gggtgattct ccctaccggt gctcactccc aaggtagact gcttgtaatc cgacaatcgt
      241 ctttgcgtcg cgtatcgttc ccgtcttaat catcgtcatc gcctcgtaca gcggcatctc
      301 aatgacttca aggacttcgt cacgatccaa ctgttgccgc cccttcatca agccagtggc
      361 tttgtagatg cgaatgacct cgtcggtaaa ccctggggca gtgaagatgc tggagagcag
      421 ctcgaatgat ccagcccggt aaccgatttc ctcctcaagt tctcgagctg cgcaggccat
      481 aggctcttcg cctggcgaga gcttcccagc cggaatctcg taaatgaatc cccctgccgc
      541 gtggcgaaat tgacgaatca gcacgacggt gccgtcctct ttgagcggga caatcgcgga
      601 ggctccggga tgacgcacga tctccaagtc gactgtgacg ccattcggaa gcgtaaccgt
      661 gtcaacgttc atcgtgatga ctttgcctgt ataaatgttc ttggtcatga agcgcgtctg
      721 gctgatggtc tatggtctgg cagaagctga atggagttcc atcgtgcctt gtcctagctc
      781 tcagccatga gccatacgac atcaaccctt gggctttttg tagaacgggg ccttgacgac
      841 gactgccggc acagcttttc cacgaatgtc gattgcgagt gcagtcgccg gggctgcgta
      901 tgaaggagga acgtagccga gtccaatacc cttctgaagc aacggtgaga aattaccgct
      961 cgtgacttca ccaatgaccc gcgacgatac agaatcgaga atcttgaacc cgtgccgagg
     1021 cacagccttt tcgacaagtt cgaaggccat gaatcgccga ggcacgccgg cctctttctg
     1081 tgtgagcaac gcctggctgc cgataaagtc gcccttctgg aagctgacgg tccattcgac
     1141 gttggcctca agcggtgtcg tctcttcgtt gatatcgttc ccgtagagga gataacccat
     1201 ttccagtctg agtaagtctc tggcgcccag tccagccggt ttgagccccc atgccgaccc
     1261 cttctgcatg aatagatccc atacgcgacc gaccttgtcc gcgtcgacgt atatttcgta
     1321 gcccagttcg cctgtgtacc cggtccgcgc gatgaggcac ggaaatccgc cgaggttggc
     1381 ttggcaggca tggtgaagtt tgagcgtggt gaggattgtt cctccgagac tgacgagcaa
     1441 ctcgcgagac tgagggcctt gaatggcgat ctgagacaac cgcgtcgaac gatcttgaag
     1501 aataactgcc ttcgttgatg cgaggtggct tcttaaccag gagagaatct tttcgcggtt
     1561 cgaagcgttg acgcataaga ggaaatcttc cgaagcgatc cggtaaataa aaatatcgtc
     1621 tttgatcccg ccttgctcgt tgcagaccgt ggaatactgg gcttgggaca cagtgagttt
     1681 cccaacgtcg ttggttgtca ctcgctggag gaatgccacc acgtcggcac cggagagcca
     1741 aagccggccc atgtggctga cgtcaaagag cccgaccctc gtccggacgg tctggtattc
     1801 atccacgaca ccgctgtatt gaatgggcat ttcccagccg gcgaagtcga caagcttggc
     1861 accggcggat tgatgttgtg taatgagcgg cgtgcgctgc atggcctttt cacacaccga
     1921 ggagttcgac atcgaagatc aaggtcgcgt ggggaggaat gatgccgccg gccccgcggg
     1981 cgccatatcc gagattggca ggaatcgtca gcctccgctt gccacccacc ttcatgccct
     2041 ccacaccttc gtcccatcct ctgatgacct gcccggcccc aaggcgaaat gagaagggct
     2101 ggcctcggtc aacggagcta tcgaattgtt tcccattctc cagccagccc gtgtaatgga
     2161 ccgttgcggt ctttccggca acggcgactt caccggtacc gacgacctca tccctatacg
     2221 tgaggccaga tgatgtggtg atttcgttct tgccagtggt cttgtcgctc cctttcatgt
     2281 ctcctcccgt tccgatggtc ggtggtcggc ttgcatcccg gttttccctc tggacgatct
     2341 gctgaaagat tttggcaggc ggccggtccg ttcacgaatc cggttctgcg aacaacgcca
     2401 cactggccgt gtagccatga aggaagtttc gtcctccgac cggaccaatt tctccttggg
     2461 cgaaaaatcc tgccacgggg atgtctccaa atcgctcgtg gatcgcgccg ctgtcgtgat
     2521 gcggataacc gaatagccct tgcccacggc cacagcagct gaagagcagc gcgctcaacg
     2581 gaggagtgcg atgtatcgta cgatccttgg caagcaggac ctgcaaatcc tcgctcgcgg
     2641 atatgccgtc gcgaagttga aactgaacag tctggccttc ttcaacgaca tcgccgatcg
     2701 cgatcgcacc ggcctgctgg tctgcgccaa tcaagttccg aacgaggaag tctcctcgct
     2761 caaagtgatt tcggtgctca tcgatgacaa taccgaggtg aagagccagg tgtgcgtctc
     2821 gccggtgggt gctctctagt gattcaaaga cgtcctggag ccgttgaagc gccggctttc
     2881 cgcctagctc gtaaaccaaa tttcgctccg ccttggtgac gacgaatcga tcgccgatcg
     2941 gacggcaacc ttgtgagatc accgttcgga ccgagactgg accgtccagt cgtacgccga
     3001 cgagacctcc gtcgaagacc tcgtggttga gcagcaaccg gtttgcacca acatcatggc
     3061 ctcccccggc cagacctcca atagccttgg cctgtggata ccgatccgcc atcaccgaca
     3121 gcacatcccg catgggtgta gtaaaaggat cggcgaccag gataaacgtg gattcggccg
     3181 cagtggattc aggccagccg gttatttgga tctgatcccg cacgtcagaa aaagacaacc
     3241 ggagtggtgt catgtggact tgaggcaggc gtgccaccca aagcgtgatc gccggtgctg
     3301 tttcgagttc ctcagatcca ccgatcacac cttccccaga gcaaccgaca cagacctctg
     3361 gctgtaacgc ctcttggagc atagttgaga tgacggtggc gtgtgccaca tggtgggcag
     3421 agaagaatgc gaacgcgaga tccacagagt tctcgccgat ctgccggcgg acgatgtccg
     3481 cgacatcacg agccgccgct tccgtatcgg cacttcgaga gagcgccgac gcaaattgga
     3541 gcgtgctgtt gtgtgtggcc cgggaagaat gggtgatcat cgtataggcg tcgccgaacg
     3601 gtcagacgcc ggggtccatg cgttccgctc ggtccttggc gagcttcttg tagtatcgcc
     3661 acatgtagga atagtagtcg tcgagttgtg ccagaagata gtgcaattcg gtcgggtctt
     3721 gggattcgag cgcacggacc atcctcgtct tcaagaattc accgaaggca tcagtttttt
     3781 ccacagcggt caggagccgg agcccgccgc cgatttggta ttcgttcatg acattcctcg
     3841 accgacctcg ggagcaggat agcgagacga tggagggaaa tgcaagagag cgagatgagg
     3901 caggacagag agacgcatgg tcagatgact atcgcgtctg gtggccggta atcaaggacc
     3961 ggagccgatc caacgaaatc gcctcgacac ggttcccgtc gcgcccggtc accgaggtgg
     4021 ccatggtcaa cgcattaagg atcgcttcct ccgttgcttc gacagtggcg gtaatcagag
     4081 gattcagata
//
LOCUS       DATDNW010000232         3719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_794756, whole genome shotgun sequence.
ACCESSION   DATDNW010000232 DATDNW010000000
VERSION     DATDNW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794756"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VI359_05730"
     CDS             complement(<1..691)
                     /locus_tag="VI359_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEY6973786.1"
                     /translation="MDLLIRFVDTVLLRPYVFVFLAVFLLAGVQLIGWPRTWRFWIIS
                     WASAFVCEFSSTRVGIPFGWYDYTGSTVGQELYLSNIPFMASLSFSFLLYAAYCAALA
                     LLLPIDHASTPAPPSLIPLRFDPAARTSRPVFLITSFLFALIDMVIDPVALRGDRWFL
                     GRIYYYVDPGFHFGVPLANYVGWVVVGLICLSIYFPIDRRLPGPPSHTNGSTTLRLLP
                     GIGLYYVIVAFN"
     gene            complement(813..1796)
                     /gene="hpnA"
                     /locus_tag="VI359_05735"
     CDS             complement(813..1796)
                     /gene="hpnA"
                     /locus_tag="VI359_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEY6973787.1"
                     /translation="MKVLVTGASGFVGGAVARTLVQAGIDVRVLARPGSDPQNYARLN
                     VETVEGELRDPNSLRRALTGCRQLYHVAAHYALWAKDPAIFYDVNVTGTRTLLEIARD
                     VGTERIVYCSTIGAIGLPPGGGLGTEDTPVSLDQMAGHYKRSKYLAEQEVLKLAREGL
                     PVVIVNPSAPVGAGDVRPTPTGQVIVDFMKGRMPAYIETGMNIVDVDDVATGHLLAMQ
                     KGRIGERYILGTKNLMLREVFEILSRLTGVKAPSIKLPRLAILPLAYLNEWIATLTGQ
                     PPRIPLEGVKMAKYKMHYDCSKAIKELGIPQTPPEVALEKAVRWFTSHGYA"
     gene            complement(1907..2854)
                     /locus_tag="VI359_05740"
     CDS             complement(1907..2854)
                     /locus_tag="VI359_05740"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY6973788.1"
                     /translation="MLRSAPIRRRATTNPIFAFMNIKDYLEEKRIAVDRFLDQVSPQP
                     ATPPTTLHESMRYSLMAGGKRVRPILAIAAADAVGTTPPGIMAIACSLEFIHTYSLIH
                     DDLPCMDNDDFRRGKPTNHKVYGEAMAILTGDALLTMAFDLCSHPDLMKGCDPTRQVR
                     LIQELAYGSGNMGMVGGQVFDIQAENKDIDLPTLQNIHKHKTGMLIRAAVRMGAIAAG
                     ANDRQLDHLTGYAEDIGLAFQIADDVLNVTGTREELGKNPNTDAERGKKTYPTFYGVD
                     GARKLADECVARAINRLSSFGPAADPLREIARYITSRRS"
     gene            2981..3271
                     /locus_tag="VI359_05745"
     CDS             2981..3271
                     /locus_tag="VI359_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973789.1"
                     /translation="MSIRKAGGDWEPMLLGIEPRNCKVCKLGLYRDKTMFRGGWSRCT
                     VCDEFVHYSCLASGKVSFLKARPRVCKTCRAAKEGAPSSSTTKDEAPVAVGS"
     gene            3268..3603
                     /locus_tag="VI359_05750"
     CDS             3268..3603
                     /locus_tag="VI359_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973790.1"
                     /translation="MSSSTSSHPDHVRSGWHTVFAEAVRLALAPLILLFSGFFTANFI
                     VSGQYTWPRTSRAVALTLTVLILSYEFVYKEQLACAAAPGRARTAVVYSCAIPYLAGA
                     LVMLALWKL"
BASE COUNT          788 a         1034 c         1121 g          776 t
ORIGIN      
        1 ggttaaaggc cacaatgaca taatagagtc caataccagg gaggaggcga agtgtggttg
       61 atccgttggt gtgagatggc gggccaggga gtcgccggtc gatgggaaag tagatggaaa
      121 ggcaaatcaa cccgaccacg acccatccga cgtagttggc gagcggcaca ccgaaatgga
      181 atccgggatc gacgtagtaa tagatcctgc cgaggaacca acgatcccca cgtagcgcca
      241 ctgggtcgat caccatgtcg atcaaggcga acaagaagct cgttattaga aaaaccggcc
      301 gacttgtccg tgcagccgga tcgaaacgca aaggtattaa ggacggcgga gcgggcgtcg
      361 acgcgtgatc gattggaagg agcaaggcga gcgccgcgca ataagccgca tagaggagaa
      421 agctaaatga gagcgacgcc atgaagggaa tattcgaaag atagagttcc tgcccgacgg
      481 tggaaccggt atagtcgtac cagccgaagg ggattccaac gcgagtggag gaaaattcgc
      541 acacgaaggc gcttgcccag ctaatgatcc aaaaccgcca ggttcgcggc cagccgatta
      601 gttgaacgcc agcaagaaga aacacggcca ggaacacgaa cacatagggc cggaggagga
      661 cggtgtcaac gaaacggatt aggaggtcca tcactagcga ataattaatc ttgaatgatg
      721 cattcttgca aggggtcccg caattttcaa ttcaatactg aacattgagc attctccaac
      781 ggttatgcgt tgccggcacc ttccacgacc agttacgcat agccgtgaga cgtgaaccat
      841 cgcacggcct tttccagagc gacctcgggc ggggtttgcg gaatgcccaa ttccttgatt
      901 gctttactgc aatcgtaatg cattttgtac ttggccatct tcaccccctc aagtggaatt
      961 cgcggtggtt ggccagtgag ggtggcaatc cactcgttca gataggcgag cgggaggatc
     1021 gccaaccgag gaagcttgat cgaaggcgct ttcacgccag tgaggcggct aagtatctcg
     1081 aacacctcgc gcagcattaa attcttggtc ccgaggatat accgttcgcc gattcgtccc
     1141 ttctgcatgg ccaacaggtg accggtcgca acatcgtcga catccacgat gttcattcct
     1201 gtttcgatgt aggccggcat ccggcctttc atgaaatcca cgatgacctg ccctgtcggg
     1261 gttggccgga cgtcgccggc accgaccggg gcactgggat tcacgattac gaccggtaag
     1321 ccttcgcgtg ccagcttgag tacctcttgt tcggccaggt atttggatcg cttgtaatgg
     1381 ccggccatct ggtcgagcga cactggcgtg tcttcagtgc cgagtccacc gcccggcggg
     1441 agaccgatgg ctccgattgt gctgcagtaa acgatccgtt ccgttccgac atcgcgggca
     1501 atttccagga gcgtgcgggt gccggtcacg ttcacgtcat agaaaatggc agggtctttc
     1561 gcccacaggg cgtagtgggc agccacgtgg tacagctgcc ggcaaccggt gagtgctcgt
     1621 cgaagagagt tgggatcgcg caactcgcct tcgaccgttt cgacattgag tcgcgcgtaa
     1681 ttttggggat cgctaccggg gcgcgcaagc acacgcacgt cgatcccggc ttggacgagt
     1741 gtgcgggcaa cggcccctcc gacaaagccg ctagcgcctg tgacgaggac cttcatcgat
     1801 tatacgcagg tgaggggcta ggggcgagag gcgagaggtg ctgataggag agtgaaatcc
     1861 ttctctggcc catagcccct gacctcacgc ctcttgccac gcgccgctag gagcggcgcg
     1921 acgtgatata gcgcgcgatt tcccggagag gatcggcagc aggaccgaag gacgacaacc
     1981 gattgatggc acgcgcgaca cactcatctg cgagcttccg cgcaccgtcg acaccataga
     2041 acgttggata ggttttcttc ccgcgttccg catcggtgtt gggatttttg ccgagttcct
     2101 cgcgcgtgcc ggtcacgttc aagacatcgt cggcgatctg aaaggccagt ccgatgtcct
     2161 cggcgtaccc ggtcaggtga tccagctggc ggtcgttggc cccggcggcg atcgccccca
     2221 tccggacagc ggcgcgaatt aacatcccag ttttgtgttt gtgaatgttc tgtaaggtcg
     2281 gaaggtcgat gtctttgttc tctgcctgga tatcgaatac ttgaccgccc accatcccca
     2341 tgttgccgga cccgtacgcc agctcctgaa tcaacctcac ttgacgcgtc ggatcgcagc
     2401 ctttcatgag atccggatgg ctgcacagat cgaaggccat ggtcaggagc gcatcgccgg
     2461 tgagaatggc catcgcttcg ccgtagactt tgtgatttgt tggtttgccg cgccgaaaat
     2521 catcgttgtc catgcaaggg aggtcatcat ggatcaagga ataggtatgg atgaattcca
     2581 gcgaacaggc gatggccatg atgccgggag gcgtggtccc tacggcgtca gccgcagcaa
     2641 tcgccagaat cggacgcacc cgcttgccgc ccgccatcag gctgtagcgc atgctctcat
     2701 gaagcgtggt cggcggcgtg gcgggctgcg gactcacctg gtccaagaac cgatcgactg
     2761 cgatccgttt ttcttcgaga tagtccttga tgttcatgaa tgcaaagatg gggttcgtcg
     2821 tggcccgacg tcgaatcggc gcggagcgta acaaatgggt atcgggctgt caaacaagcc
     2881 ggcaggtatt gtcataagct agggccacaa gatcaagaga accacctggt ctgacgacac
     2941 ttgccccgtg tcgactccct tcgctatact ccatctcgct atgagcatca gaaaagccgg
     3001 cggtgactgg gaaccgatgt tgttggggat tgagccccgg aactgcaagg tctgcaagct
     3061 cggtctctat cgagacaaaa ccatgttccg tggcgggtgg tcccgttgta ccgtctgcga
     3121 tgaattcgtc cactacagtt gtctcgccag tggcaaagtg tcattcctca aagcgcggcc
     3181 tcgtgtgtgc aagacctgcc gcgcggccaa agagggcgcc ccctcttctt cgacgacgaa
     3241 ggatgaggcc ccggttgcgg tcgggtcgtg agttccagca catcgtccca tcccgatcac
     3301 gttcgctccg gatggcacac cgtctttgcg gaggccgttc ggttggcgct ggctccgctc
     3361 atcctgctct tcagcgggtt tttcacggcg aacttcatcg tcagtgggca gtacacgtgg
     3421 ccgagaacga gccgggccgt ggcgctgacg ctcaccgtgc tgattctctc ctatgagttt
     3481 gtctacaagg aacagctcgc ctgtgcagca gctccgggcc gggcccgtac ggctgtcgtc
     3541 tattcctgtg cgatcccata cctagccggc gcgctcgtga tgctggccct ctggaaactg
     3601 tagcgagttt cggttttcta ccggtggcaa ggacaatggg agagatcatc cgcagcggag
     3661 ccttcctgca acaatgttgg tcggtccatc cgttgtgtgt agcggtcaag cggatggtt
//
LOCUS       DATDNW010000233         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1257173, whole genome shotgun sequence.
ACCESSION   DATDNW010000233 DATDNW010000000
VERSION     DATDNW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257173"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2246
                     /locus_tag="VI359_05755"
     CDS             <1..>2246
                     /locus_tag="VI359_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973791.1"
                     /translation="FGDVPTTIPRGYLGDPVKWRLVHGGSEVFHSHHPHSGSIRWQRS
                     PGTEPNNLWAAGQDGPVKYPIIRTKSDRVDVEVIGPSESLDLEPECGGGGCQHLAGEF
                     LFHCHVAHHYVAGMWGYARFYNTLQAGVGHTDTMPDLRELPDRQGRMKLGVSSDKLIG
                     TTLDWFGKTFKIVDKSQKTNWKSDPAVVNIKDWVEMWIPAAGKPGHTDNEKAQIMAYD
                     ATVWDWKWNGNVAVGERESTAQNPKYPWAAKWDDSTRPTILFDPTTGRMSWPLFKPHY
                     GKRVPFSANHNGAPWLEPIHQDSKGERTSEPAKPGEQGNWSLCPVNANQKFYNIHFVR
                     MPITLAKQQGKEPPIVDKDGLIYVLHEEERLTRANDDLKLPAVIRANVYDCVDLVLTS
                     EWDDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGFEYEMSVRPFTMWGKSKKHGLPA
                     PMVAKIVGGAKAGANSIKIQMAPGATPFHVNTELMVGMDCLEKGNDPAASLPRDKSCS
                     EVARIKDIKGDQVTFFTPLKHNHPANDLVSPEFVRYRWWVDVDMGTVFWHDHAFGATT
                     WPHGGFGVTIVEPFGSTYHNPKDGKLVYSGPIADIHSNEPIGAGVSGSFRELMVSIHD
                     TVPHTVNVIQAGNPPGQPVEVALEAGKTVSFQMPDKILNAPNQYINGGTHTTGSGFNF
                     RAEPFAQRLSNNPDTSKLFSSAIHGDPDTPLLRAYTGDTMVFRLLHQLMNESHVWTIA
                     GHTFLTER"
BASE COUNT          510 a          632 c          674 g          430 t
ORIGIN      
        1 cttcggcgat gtgccgacga cgattcctcg cggatatctc ggcgatcccg tcaagtggcg
       61 gttggtccat ggtggatcgg aagtgttcca ctcccaccac ccgcacagcg ggtcgatccg
      121 ctggcaacgg agcccgggca cagagcccaa caatctgtgg gcggccggtc aggatggtcc
      181 ggtgaaatat ccaatcatcc gcacgaagtc cgatcgcgtg gacgtggaag tgatcggccc
      241 gtctgaatcg ttagatctgg aaccggaatg tggcggcggc ggctgtcagc acctggctgg
      301 cgaattcctc tttcactgcc acgtcgcgca ccactatgtg gcggggatgt ggggttatgc
      361 ccgcttctat aacacgttgc aagccggcgt tggccacacg gacacgatgc cggacttgcg
      421 agaattgccg gatcgtcagg gccggatgaa gctcggggta tcgtcagaca agctgatcgg
      481 gaccaccctc gattggttcg gcaagacgtt caagatcgtc gacaagagcc aaaagaccaa
      541 ctggaaatcc gatcctgcgg tcgtgaacat caaggattgg gtggaaatgt ggattccagc
      601 cgctggcaag cccggccata cggataacga gaaggcccag atcatggcct acgatgcgac
      661 cgtgtgggat tggaaatgga acggcaatgt ggcggtcgga gaacgtgaaa gcaccgctca
      721 gaatcccaag tatccatggg cggccaagtg ggacgacagc acgaggccga cgattctgtt
      781 tgatccaacg acaggcagaa tgtcctggcc gttgttcaag ccccattacg gcaagcgtgt
      841 gccgttctca gcaaatcaca atggtgcgcc ctggctggaa cccatccatc aggatagcaa
      901 aggtgaacgg acctctgaac cggcgaagcc gggcgagcag ggaaactgga gtctctgtcc
      961 ggtgaacgcc aatcagaagt tttacaacat ccactttgtc cgcatgccga tcaccctggc
     1021 caagcagcag ggcaaagaac caccgatcgt cgacaaggac ggcttgatct atgtgctcca
     1081 cgaagaggag cgcttgacca gagccaacga cgatctcaag cttccggcgg tcattcgcgc
     1141 caacgtgtat gactgcgtcg atttggtcct caccagcgag tgggatgacg acgactacac
     1201 gaatttccag tcgtccaaga tcaatatcca tcctcacttc ttccagttcg acaccgggaa
     1261 ctcagacggt gtgatctccg ggttcgaata tgaaatgtcg gtgcgcccgt tcacgatgtg
     1321 gggcaagtcc aagaagcacg ggttgcctgc gccgatggtc gccaagatcg tcggtggcgc
     1381 aaaggcaggg gccaattcaa ttaagatcca aatggcacct ggggcgacac cattccatgt
     1441 gaacaccgaa ttgatggtgg gcatggattg tttggagaag ggcaacgatc cagcggcttc
     1501 attgccacgg gataagagct gttccgaagt cgcccgtatt aaggacatca agggcgatca
     1561 ggtcacgttc ttcacgccgc tgaagcacaa ccatccggcc aacgatctcg tgtcgccgga
     1621 gttcgtgcgc tatcggtggt gggtggacgt ggatatgggg accgtgttct ggcacgacca
     1681 tgcattcggc gcgacgacct ggccgcatgg cgggttcggc gtgacgatcg tggaaccgtt
     1741 cggatccacg tatcacaacc cgaaggacgg caagctcgtg tacagcggcc cgatcgcgga
     1801 cattcattcg aatgagccga tcggcgccgg cgtcagcggc agcttccgtg agttaatggt
     1861 gtcgatccac gacacggttc cgcataccgt gaacgtgatc caggccggca atcctcctgg
     1921 acagccggtc gaagtagccc tggaagcagg caaaacggtg tcgttccaga tgccggacaa
     1981 gatcctgaac gcgccgaatc aatacatcaa cggcggaacg cacacgaccg gcagcggatt
     2041 taacttccgc gcggaaccgt ttgcccaacg cctgtcgaac aatccggata cgtcgaaact
     2101 gttcagcagt gcgatccatg gtgatcctga tacgccgttg ttgcgcgcgt ataccggtga
     2161 cacgatggtc ttccgcctgc tgcatcagtt gatgaacgaa tcgcacgtct ggacgatcgc
     2221 cggccatacc ttcctcacgg aacggt
//
LOCUS       DATDNW010000234         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_795059, whole genome shotgun sequence.
ACCESSION   DATDNW010000234 DATDNW010000000
VERSION     DATDNW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795059"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VI359_05760"
     CDS             complement(<1..578)
                     /locus_tag="VI359_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY6973792.1"
                     /translation="MGECAKRRVWFLALGILLIMSVPAQAEMYVAGQVGVTIPQNLSN
                     VEWSAGGVTVGGNDLSLHNSVMYGAKLGYYFDSLKWLGLETEAFNTTPNVKQQNLTIG
                     GINLGTQPGVDFRVLTWAPVNIVARYQMGAFEPYAGVGLGVFFSRLSQGGSSSSSTDV
                     GLNTQVGLRYRVTNNLAVFGEWKFNHANISHD"
     gene            794..1408
                     /locus_tag="VI359_05765"
     CDS             794..1408
                     /locus_tag="VI359_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973793.1"
                     /translation="MKLYADFIFPRVMDWVMSGEVFRQLRAELLKDAHGDVLEIGFGT
                     GLNLPHYPTNISNLTIVDPARLLPQRVRERVARVPFPILAEHITAEALPNPDRQFDSV
                     VSTWTLCTIPDPVKALREINRVLKPNGTFLFLEHGRSDNPRVAAWQDRINPLQNIIGC
                     GCNLNRRIDQLIEQSGLHILHLDRFLMPNVPRMFGEMYQGRASS"
     gene            complement(1421..2113)
                     /locus_tag="VI359_05770"
     CDS             complement(1421..2113)
                     /locus_tag="VI359_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY6973794.1"
                     /translation="MAQPSAFNLLPSLDWNQIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAMQGLSFQSARDHLMAMYRSVEGELAWTDLHYWTKRVGIDVVAMHKELDYMIGF
                     LPGAEAFLSELRAKGKRITVLTNAHRAGVIVKSAKTGLGRYVDRIVDAFEVGYLKMRP
                     EYWPTCKRLVGFNPARSLYVDDDESCLGAAKQFGIAGIVHSAKSSSQLPPAHSTRFAS
                     IEQLSQLVNGKW"
BASE COUNT          553 a          636 c          580 g          548 t
ORIGIN      
        1 ttatcatggc taatattcgc atgattgaac ttccattctc caaagaccgc gagattgttg
       61 gtcactcgat acctcagtcc aacttgcgtg tttaatccga cgtccgtgct gctacttgag
      121 ctgccacctt gagataggcg ggaaaagaat actcctaacc ccactccagc ataaggctcg
      181 aatgctccca tttgatatct agcgacaata ttcactgggg cccaggttaa gactctaaag
      241 tccacaccag gttgggtccc gagattaata ccccctattg tcaagttttg ctgtttaaca
      301 ttgggcgtcg tattgaaggc ttccgtctcc aagccaagcc atttgagtga atcaaagtag
      361 taacccaatt tggcaccgta cattacagaa ttatgcaaag agaggtcatt gccaccgaca
      421 gtaaccccgc ctgcactcca ttccacgttg ctaagattct gaggaattgt cacacccacc
      481 tgaccggcca catacatctc cgcctgagcc ggaacgctca tgatcaacaa aattcccagc
      541 gcaagaaacc aaacacgccg tttcgcacat tcgcccataa tatccctcct tggaaagatg
      601 atgagttaag ccgactagga gagacaatca tccggccgtt gattgcttcc ctaataatcg
      661 gtcgctcctg tatcatgttg ttcttatctc gtcaaacata acgtcatgat ttatctgaca
      721 aattacacgt atttcgctga tgagatgtga gggaggctaa accaggtggt tggtgacgac
      781 ggagaggtga tcgatgaagt tatatgccga ttttattttc ccacgcgtta tggactgggt
      841 gatgagcggc gaagtgtttc ggcagctgcg ggcggagttg ctgaaggacg ctcacggcga
      901 cgtcttggaa atcgggtttg gaacagggtt gaatttgccc cactatccta cgaacatctc
      961 gaaccttacg attgtcgatc cagcccgcct gctcccgcag agagtaagag agcgagtagc
     1021 gcgggtcccg tttcctatcc ttgccgagca catcacggcc gaggctctgc ccaatcctga
     1081 tcggcaattc gactccgtcg tcagcacgtg gacgctctgc acaattcccg atcccgtaaa
     1141 agcgctgcgt gagatcaatc gtgtgctcaa gccgaatggg acctttcttt ttctggagca
     1201 tggccgaagc gacaaccccc gcgtcgccgc ctggcaggat cgaatcaatc ctcttcaaaa
     1261 catcatcggc tgcgggtgca atttgaatcg acggatcgat cagcttattg aacaaagcgg
     1321 cttacacatt cttcacctcg atcgattcct gatgcccaat gtcccgagga tgttcggcga
     1381 aatgtaccag ggccgggctt cgtcataaac gagaaccgcc ttaccatttg ccgttgacga
     1441 gctgcgacaa ctgctcgatc gaagcaaagc gggttgaatg ggccggtggc agctgcgagc
     1501 ttgatttcgc actgtggacg atcccggcga tgccaaattg cttcgcggca cctaagcaac
     1561 tttcatcgtc atccacataa agcgaccgtg cgggattgaa tccgaccagc cgtttgcagg
     1621 tcggccagta ttccggtcgc attttcagat agcccacctc aaaggcatct acgatccgat
     1681 ccacgtaacg gccgagaccg gtcttggctg atttaactat caccccggct cggtgggcat
     1741 tcgtaagcac cgtgatccgt ttgcctttag ctcgcagttc gctgagaaag gcttcggcac
     1801 ccggcagaaa accgatcatg tagtctagtt ccttgtgcat ggccacgaca tcgatgccca
     1861 cccgttttgt ccagtaatgc aaatccgtcc aggccagctc gccttcgacc gaccggtaca
     1921 tggccatgag atgatcgcgg gcggattgga acgagaggcc ctgcatggcc gcatagcggc
     1981 ggggcaattc ttcttcgaaa aagaaattgt cgaaatgtcg gtcgagcaag gtcccgtcca
     2041 tatcgaggag gacgtcgtcg atctggttcc aatcgagaga gggtagaaga ttgaaggctg
     2101 agggttgagc catgaggaaa agtataccgg aagggctcaa gagaagacga ggtgagttgt
     2161 ctttgagaaa aaccctatgt tacggtcctg ccatcgtgca tccatctgat tccatgccct
     2221 ccccctccat ccttagcgct cattccgcag cactcgtgcc tttggtcgct atcatcggta
     2281 ggccgaacgt cggaaaatcg acgctgttca accgcct
//
LOCUS       DATDNW010000235         2856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_987372, whole genome shotgun sequence.
ACCESSION   DATDNW010000235 DATDNW010000000
VERSION     DATDNW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987372"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..580)
                     /locus_tag="VI359_05775"
     CDS             complement(98..580)
                     /locus_tag="VI359_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973795.1"
                     /translation="MKIQRQWVLVGALVMGVAACVGTSHGERDHVTPGDPVIGLLSKG
                     ITQLNGNINALSKRMNDVQQASAGTDPALRELQALDLSGWQLHQQQWVLQRDHLVLAR
                     DSLQQASGQPEVKKQLLEQWRQHQQRYVNALEELRQQRQNLENKHLEVEARLIERGLQ
                     "
     gene            complement(635..1153)
                     /locus_tag="VI359_05780"
     CDS             complement(635..1153)
                     /locus_tag="VI359_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973796.1"
                     /translation="MDRTAEGKSPFRTWPARYGLLLSACAVLVGCVSVRVEPLVKESY
                     PRRENHSPVQWLETEPGSPHIKLARIVATSQTADEDRMRDKILARAESMGADAVVLGR
                     DDVVESMMGPSPLYESTLGPAADSFSPYASGWGWWNPFYYDPWSFVQGAADQTGRTEL
                     LSGTAIRYLNGK"
     gene            complement(1360..1926)
                     /locus_tag="VI359_05785"
     CDS             complement(1360..1926)
                     /locus_tag="VI359_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973797.1"
                     /translation="MLLSMHESWRQPSMKRSCTILSTLGLYLMVSACTRVDTILLTSD
                     TFPPKSSADEVAVLEQNPTRPHRDIAELRIGDSSLSFGSLQRKILSRAATLGADAVVF
                     AKPQTQTTHQVAYEPVYDPWGYNSPYYGGPWGYGGGYGGPFGGWGPWGGMYSGSVAVP
                     YNETVRMLMGTAIHYTQPMDSSGHKVNE"
     gene            complement(1964..>2856)
                     /locus_tag="VI359_05790"
     CDS             complement(1964..>2856)
                     /locus_tag="VI359_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6973798.1"
                     /translation="NLKKLPRRMVVIGGGIIGIEYASMLAALKINVTLIDKRTRLLEF
                     LDSEIVEELMHQMRNRNVTFRLGEAVERLEVTSDEPHRAVITLESGKRIVSESVLYSV
                     GRVGATERLNLTATGLTADKRGRLTVDAQFRTEIPHIFAAGDIIGYPSLASTSASQGR
                     HVACYAFGVEIEPLPEHLPVGIYAIPEISMAGPPEHELTEQHVPYETGMARYREIARG
                     QILGDDSGLVKLLFHREDRRLLGVHAIGTGATELIHIGQAVLDLGGGLNYFLRTVFNY
                     PTLAECYKVAALDAFNKLSM"
BASE COUNT          609 a          844 c          741 g          662 t
ORIGIN      
        1 cccgagtaac tccggatgat gaccattagt tgtttgaacc attcattctt ctaagtagcc
       61 tggcgagaca gtttattcga agcattcact aggccattca ctgaagcccg cgttcgatta
      121 agcgcgcttc cacctccaaa tgcttgttct ctaagttttg tcgttgttgg cgaagttcct
      181 ccagtgcatt cacgtaccgt tgctggtgct gtcgccactg ttccagcaac tgcttcttca
      241 cttccggctg gccggatgct tgctggaggc tgtcgcgtgc caagacgaga tggtcgcgct
      301 gtaataccca ttgttgctgg tgcagctgcc atccggacag atctagagct tgtaattccc
      361 gcagcgcggg gtccgttccc gccgacgcct gctgcacgtc attcatgcgt ttcgacagcg
      421 cgttgatgtt gccgttcaat tgggtgatgc ctttgcttaa aaggccgatg actggatcgc
      481 caggggtgac atgatcgcgc tccccatggc ttgtgccgac acaggcagcg acacccatca
      541 cgagcgctcc gacaaggacc cactgccttt gtatcttcat atgtcctcct tcaagaaaca
      601 ctccgacaat ctcgattccg ttaggatgtc agctctactt tccgttcagg tatcgaatcg
      661 ctgtgccaga caataattct gtccgcccag tctggtcagc agctccttgg acgaaactcc
      721 agggatcgta gtagaaggga ttccaccacc cccagccgct tgcatatggg ctaaagctgt
      781 ctgcggccgg tcccaaggtg gattcgtaga gaggactcgg tcccatcatt gattccacaa
      841 catcgtctct tcccaacacc accgcatcgg ctcccatcga ttcggcacga gctaggatct
      901 tatcccgcat ccggtcctca tccgcggtct gactcgtcgc aacaatccgt gcgagcttga
      961 tgtgcggaga acctggctct gtctccagcc attgcacggg gctatgattc tcgcgccgcg
     1021 gatacgactc tttgaccaac ggctcgacac gcactgacac gcacccgacc agaacggcac
     1081 aggcggacag gagcaggcca tagcgcgcag gccacgttct aaatggagat ttaccttctg
     1141 ctgtccgatc catcgcgtga ggacgtcatc ccgtgtttaa tacaccccgg caaggaccgg
     1201 cccccggtct cttccacatc gtcagctcag cagaaccaag aagaaggaag ctgtgagctg
     1261 cagcattctc tgcacaggtg ttttttcgaa cgccttgccg ggagcctcgg attcacaaat
     1321 aacaattacg tgtctgaaga atccttagtg atgacgagct cattcgttga ccttgtgacc
     1381 actcgaatcc atcggttgtg tataatggat ggctgttccc atgagcattc tgacggtctc
     1441 gttgtacgga actgcgacgc tgccggaata cattccaccc caaggacccc atcctccaaa
     1501 gggaccgcca taccccccgc catagcccca agggcctcca tagtatgggc tgttgtagcc
     1561 ccatggatca tagaccggtt catatgccac ttgatgcgtc gtttgagttt gcggcttcgc
     1621 aaacacgacg gcatcggctc caagagttgc cgcccgactc aaaatctttc gctgcaagct
     1681 gccgaagcta agcgagctat caccaatccg tagttccgcg atgtcccggt gaggtcgcgt
     1741 agggttctgt tcgagcacgg cgacctcgtc cgcagagctc ttgggtggaa atgtgtcgct
     1801 agtcaacagg atggtgtcca cccttgtgca tgcggagacc ataaggtaga ggccaagcgt
     1861 cgacaggatc gtgcaggatc gcttcatgct cggctgtctc caactctcat gcatagatag
     1921 gagcatgaca ggctcccacg aagttccaat ctgatgcgga cgactacatc gacagcttgt
     1981 tgaatgcgtc cagcgccgcc accttgtaac actccgccaa cgtcgggtaa ttaaagacgg
     2041 ttctcaggaa atagttcagc ccaccgccga ggtcgagcac agcctgtccg atatgaatga
     2101 gttcagtcgc gccggtcccg atcgcgtgaa caccgagcag ccgtcggtcc tctcgatgaa
     2161 aaaggagttt taccaagccg ctgtcgtctc cgaggatttg gcctctagcg atctcccgat
     2221 accgggccat gccggtttcg tagggaacat gctgttcagt cagttcatgt tcaggtggtc
     2281 ctgccatgga tatctctggg attgcgtaaa tgccgactgg taagtgctcg gggaggggtt
     2341 cgatctcgac cccgaatgcg taacaagcga catgccgacc ctgcgaggca gaggtggagg
     2401 caaggctggg ataaccgatg atgtcgcctg cggcaaaaat atgcgggatc tcggtccgaa
     2461 actgagcatc cactgtcaac cgcccacgtt tatcagctgt caaaccagtt gccgtaaggt
     2521 tgagtcgctc agttgccccg actctcccga ccgaatagag cacggattcc gacacgatgc
     2581 gcttgccgga ctccagggta atcacagctc gatgcggttc atcgctcgtc acttctagcc
     2641 gttcgactgc ctcgcccagt cgaaacgtga cgttgcgatt gcgcatctga tgcatcaatt
     2701 cctcaacaat ctcagaatcc agaaactcta agagccgcgt acgcttgtcg atcaatgtca
     2761 cattgatctt cagcgcggcc agcatcgatg cgtattcgat gccgatgatg ccgccgccaa
     2821 tcacgaccat tcgtcgggga agtttcttga ggttca
//
LOCUS       DATDNW010000236         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_575690, whole genome shotgun sequence.
ACCESSION   DATDNW010000236 DATDNW010000000
VERSION     DATDNW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575690"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1448)
                     /locus_tag="VI359_05795"
     CDS             complement(276..1448)
                     /locus_tag="VI359_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY6973799.1"
                     /translation="MDNIKHRFFAPCPRGLEGILEQELHELGVPKTNKTAGGVGFQAP
                     WVSMYWVNLKSHIASRVLWEVGHNDYRSEEDVYRAAYALAWPDWFMPFHSIKVKVSAH
                     RCPLPSLDFLTLRIKDAVCDKFMAVRHKRPSVDTQQPNIRLDAFLDSNSVTFYLDTSG
                     EPLFKRGHRIASVEAPLRENLAAGLLRLAGWTADEVLLDPMCGSGTIPLEAALMSRRI
                     APGLARSFAFERLQTHDPKRWGQLREAARKKERAEVPAAIYASDHDADAIKVAQRTFQ
                     GAGVALDIRLQHIDMLSIEPPADNGVILINPPYGVRLSRSVELESFYPKLGDWLKQRF
                     SGWRAYVFTGDSHLQKLIGLSPSRRIPLFNGSLECRLYEFRIVRGSGRQRRLRSSI"
     gene            1438..2016
                     /locus_tag="VI359_05800"
     CDS             1438..2016
                     /locus_tag="VI359_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6973800.1"
                     /translation="MLSIRSACYRRTPMNYCGECGKSVSKKIPQGDNLPRFVCDFCQA
                     IHYHNPKIVAGCIPEWKGQILLCRRAIEPKSGLWTYPAGFMEIGEGTEEAARRETLEE
                     AHAQVAITRLHSVLSLPHIGQVYLTFVGRLLAKEFRAGQESLDVRLFDRTDIPWNEIA
                     FPVVKDALRRYVDDVAGGEFRLHVADLPDPLI"
     gene            complement(2046..2309)
                     /locus_tag="VI359_05805"
     CDS             complement(2046..2309)
                     /locus_tag="VI359_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY6973801.1"
                     /translation="MAWYRLMYQAGVVEDLSAAGSPMAQRLFEKTKWIASNVDNLRHE
                     NLAPDLPRLHKYAVGDWRIFYAIDRTEQLVDIHAIVHKDALHK"
     gene            complement(2316..2519)
                     /locus_tag="VI359_05810"
     CDS             complement(2316..2519)
                     /locus_tag="VI359_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973802.1"
                     /translation="MDLSSLTLEQFRELVRGLVDDRLRELIGDPDLGLQLGDALRARL
                     KASLVKGERLSGDELAEKLGLRW"
BASE COUNT          634 a          708 c          764 g          595 t
ORIGIN      
        1 gcctgtgtcg ggaggtccca gccacgcaaa gaaactttga accataccgc atccgggcag
       61 cacgagaagg atcagcagat tgagcagctg cagtgtaagc cgaatcacgt cacgcgaaat
      121 cctgggcgag gaacaacatt gaggatgtgt accttatcac gcttatcatt ggaagggtta
      181 cacatggaga gatcaacttg aggagcaggc tctccgtagc atggacatgg ttcatgcggc
      241 gtccagccaa agggatgaga acagggattt tccaattaga tggaggaacg gaggcgtctt
      301 tgccttccgc tgccgcgcac gattctaaat tcgtataggc ggcattccag tgagccattg
      361 aacaacggga tacgacggga aggagagaga ccgatcaatt tctgaagatg tgagtcaccc
      421 gtaaacacat aggcccgcca tccagagaac ctctgcttga gccaatcacc cagcttggga
      481 taaaaggact cgagctcaac tgagcggctg agccgcacgc cgtatggagg gttgatgagt
      541 attacgccat tgtcggcggg gggctcgatc gacagcatgt cgatgtgctg gaggcgaata
      601 tcgagtgcga ctcccgcacc ttgaaatgtg cgttgcgcaa ccttgatggc atccgcgtca
      661 tgatctgagg cataaattgc ggccggaact tcggcccgtt ctttttttct cgctgcctca
      721 cggagctgcc cccatcgttt ggggtcatgg gtctgcaaac gttcgaacgc gaaggaacga
      781 gcgagtcccg gtgcaattcg acgcgacatc agtgccgcct ctagcgggat agtcccgctt
      841 ccgcacatgg gatcaaggag gacctcatcg gctgtccacc cggccagtct gagcagacct
      901 gcagcaaggt tttcacgcaa gggggcttcg accgaagcga ttcgatggcc tcgtttgaat
      961 agcggctcgc cggacgtgtc gaggtaaaac gtgacgctgt tcgagtctaa aaaggcgtct
     1021 agtcggatat tcggttgctg cgtatccacg ctcggccgct tgtggcgcac cgccatgaat
     1081 ttgtcgcaga cggcatcttt gatacggagt gtcaggaaat ccaaactggg aagtgggcat
     1141 cgatgtgcgc ttaccttcac tttgatgctg tggaatggca tgaaccagtc gggccaggcc
     1201 aaagcatagg cggcccgata gacgtcctct tcagagcgat agtcgttgtg gccgacttcc
     1261 cagagcactc tgctggcgat gtggcttttg agattcaccc aatacatgct gacccagggg
     1321 gcctgaaagc cgacgcctcc agcggttttg ttcgtcttgg gaacgccaag ttcatggagt
     1381 tcctgttcta ggatgccttc gagacctcgc gggcatggtg caaaaaaacg atgttttatg
     1441 ttatccattc gaagcgcctg ttatcggcga acgcctatga actattgcgg cgaatgcgga
     1501 aagtcggtct cgaaaaagat acctcaggga gacaatcttc cccgatttgt ctgcgacttc
     1561 tgccaggcta ttcactacca taatccaaag attgtcgctg gctgtatccc ggaatggaaa
     1621 gggcagattc tcttgtgccg ccgcgcgatt gaaccaaagt cgggtctctg gacatatccc
     1681 gccggcttca tggagattgg cgaaggaacc gaggaagccg cacggcgaga gacattggaa
     1741 gaagcccatg cacaggttgc aatcacaaga ctacactcgg ttctaagcct tccccatatc
     1801 ggccaggtct acctgacttt tgtcgggcgg ttgctggcaa aagaattcag ggcgggccag
     1861 gaaagtctcg atgtacggtt gtttgatcgg acagacattc cctggaacga gatcgctttc
     1921 ccggtggtga aggatgcgct gcgccgctat gtcgacgatg tggcaggcgg cgaatttcgc
     1981 ttacacgttg ccgacctgcc tgatcccttg atctgacgag taaccgatca cgtttgcgcc
     2041 gtcaatcact tatgcagtgc gtccttgtgc acgatggcat gaatgtcgac gagctgttct
     2101 gtgcgatcga tagcatagaa aatgcgccaa tcgcccacag cgtatttgtg caatcgaggc
     2161 aaatcagggg cgagattttc gtggcgaaga ttatcgacgt ttgaagcgat ccacttggtc
     2221 ttttcgaata ggcgttgcgc catcggcgag ccggcagcag aaagatcctc aacgacaccg
     2281 gcttgataca tcagacgata ccaggccatc aggcgctacc aacgcagccc cagtttttct
     2341 gcaagctcgt cgccggacag ccgctcacct ttaaccagag atgccttgag cctggcgcgg
     2401 agtgcgtcac cgagctggag tcccaagtcg ggatcgccga tcaactcccg caaacgatcg
     2461 tcgacaagac ctcgtaccaa ttccctgaat tgttccagcg tcagagagga aagatccatg
     2521 ggaatgaaag gatacggagc cctttcaaga aatgcaaatg agaggataga gctagttggt
     2581 ggtgtccgtc ttttccaatt gcttgatgaa gtagccgagc cgattctgtt cgggttctgc
     2641 aatgaccaaa aattcaatac caattccctg aggaccgcac catcgcactg ccgcattctc
     2701 a
//
LOCUS       DATDNW010000237         6382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_372397, whole genome shotgun sequence.
ACCESSION   DATDNW010000237 DATDNW010000000
VERSION     DATDNW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372397"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VI359_05815"
     CDS             complement(<1..900)
                     /locus_tag="VI359_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973803.1"
                     /translation="MTQTTFIGAILGGLLVLAGAVGILDSGAADVFLEATRPDFQKIS
                     LGVVGIQNTGGPDWLGGRIEEVLKKDVKRSLVFDLVDLPSLGIKVREVSNGATAIFKQ
                     AADNGVSLLVWGKASLKETGKEADATMEGFVYDSSSDEVVGGKRYAGSPSVLRLMAHR
                     FADELVFRYTGEPGIARTKITYVAEQGSARELYVMDYDGYDPRQLTADGFLNLMPRWS
                     PDRRFIVFTAYRNRNSQDIDMIELATGKRWTLISYGGLNITPALSPDGIFLAFASSYE
                     GNSELYRLDTRTKSVQRLTVNPSG"
     gene            complement(897..1895)
                     /locus_tag="VI359_05820"
     CDS             complement(897..1895)
                     /locus_tag="VI359_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY6973804.1"
                     /translation="MASQAYVQSVLWFDHEEQARFFHRLRRALLLSFVAHLIILLVVT
                     VLRLPQRGERPLAAVEVSLVSMSTPVPVKQPEPVKPVVQPKPPESKSLPAPVVKDTAP
                     ARAPVPPAGHVARDVLRDLQLPPDAPKFGDLAPAKSVALPQREAKVKVPDLPRIPDPI
                     QEPAATVSRTSKLSDDVDRELEEELKKVKQFTPAPKLEIPKEVRAPDQPTKPAPQQEV
                     RIPTAQTPETMLKISGSSGSNPYWARVQAVISSHWEPPPIDVSGQTYTVTIKFRLQRD
                     GMIKDVTIQKTSGNNYYDMAGQRAVLRPRQLPPFPSDMTDGYKDVEMVFRVGEPAG"
     gene            complement(1927..2382)
                     /locus_tag="VI359_05825"
     CDS             complement(1927..2382)
                     /locus_tag="VI359_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY6973805.1"
                     /translation="MTAFESRQRRFLAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDI
                     NLPKSATNTIKPEMRTVLTIEKDQRLYLDKDPVGVAQLEQKLKLLKLQHRDVSLYLRA
                     DREVPYGVVVQVMDGVKRAGIEKIGMVTEPVGAERVSAPQLTHTPSRKS"
     gene            complement(2379..3083)
                     /locus_tag="VI359_05830"
     CDS             complement(2379..3083)
                     /locus_tag="VI359_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY6973806.1"
                     /translation="MFQTSTVELVGSLGLVSKSVLVLLFLFSVISWAVILFKWRVFRA
                     ADRGDQRFMALLAKSKDLGDVYRQIRKMEESPCGSLFEGTAERMGIGGVDAKDGTGSA
                     PIDRHLIERTAAHLSHSQLSRLEAYLPFLATTGNITPFVGLLGTVLGIIDAFREIGAQ
                     GTASIAAVAPGVSEALIATAAGLFTAIPAVIAYNYFLTRIRRTAARMDTVTIELLAWL
                     PASTTKAKPAPVVMQA"
     gene            complement(3339..3417)
                     /locus_tag="VI359_05835"
     tRNA            complement(3339..3417)
                     /locus_tag="VI359_05835"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3379..3381),aa:Asp,seq:gtc)
     gene            complement(3542..3617)
                     /locus_tag="VI359_05840"
     tRNA            complement(3542..3617)
                     /locus_tag="VI359_05840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3582..3584),aa:Val,seq:tac)
     gene            complement(3715..4656)
                     /gene="xerD"
                     /locus_tag="VI359_05845"
     CDS             complement(3715..4656)
                     /gene="xerD"
                     /locus_tag="VI359_05845"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY6973807.1"
                     /translation="MLLHPMVERYLTHLRIEGGLSINTLEAYRRDLLKFQTYAHRVGV
                     SLPAQMTAGTVTGFLRSLREARLSHTSSARCLSAVRGWLRFLRQERVIDENPAVRLAS
                     RRRGIKLPRTLSQHEVTTLLDLPALDTREDHRDRAMVELLYATGLRVSELVSVEVAKI
                     NLDVGYLFVTGKGSKERVVPMNDQARETVRHYLHEVRPRLLKQRTSRFLFVTSRGTPM
                     TRQAFWKLLRRRVRRAGITKVISPHMLRHSFATHLLEGGADLRSVQAMLGHASIATTQ
                     IYTHVERERLKHVHDRFFPRKHRRGGVGKRSGVGSPR"
     gene            4745..>6382
                     /locus_tag="VI359_05850"
     CDS             4745..>6382
                     /locus_tag="VI359_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY6973808.1"
                     /translation="MNTKLRNLPLEPMIHRIIASYGLSDRPCRALLSLLAVLAFISAE
                     IDPSAGWRESRSVDAATAKGTPESHPALTQAKRLLDAGQAEEATTILRRFLGTSPKPE
                     FLDDTYLLLGAALYDTREYPEALKYLQQLQLEFPDSEVSDRGKLMLARVHAAMGNVDL
                     ALPILVNIRTLSKDDTAKLEAMHLSGDLYVKKKDPVRAIQAWLDEMALAPDDQAEDIH
                     ARISELINGQPDRKTLERIQELYSRTFPGDLASVRLIEFYVARGEDHQATRQIQQFLN
                     RFPTHPYAAKASELLGTLQTKLKSSQFVLAAVLPLSGKAAPFSNDVLSGIQLAVELSK
                     DRTTGSSIGLIVKDYESERPTFLDDLSRLLIDDHPIAVIGPLLSKSLPVMAELAERTK
                     TPLITPTATIPNVRRLGGYVFSTALTYSMQARRIASYAALEQGYRRFCILYPDTTYGR
                     ELARLFALEIQQHDGEIIAMESYKEGETDFNAQLKRMKAEDLKKYGLSVPVDPTKMTG
                     RQTKLDKKVLYTPGFDAIFVPGRAQEAGLIAAQLNFHD"
BASE COUNT         1398 a         1930 c         1727 g         1327 t
ORIGIN      
        1 cccggacgga tttacggtca gtcgctgaac cgatttggtt ctcgtgtcca atcgatagag
       61 ctctgaattg ccctcatagc tcgacgcaaa cgcgaggaag atgccgtccg gcgacagagc
      121 cggcgtaata ttcaagccgc cataggagat gagagtccac cgttttcctg tcgccagctc
      181 gatcatgtcg atatcctgag agttgcgatt ccgataggcg gtaaacacga tgaaccggcg
      241 atccggagac catcgaggca tcaaattcag aaatccatcg gcagtcagct gccgcggatc
      301 atacccgtcg taatccatca catagagctc gcgcgccgat ccctgttccg caacataggt
      361 gatcttcgtg cgggcaattc ccggttcgcc ggtgtaccga aagaccaatt cgtccgcaaa
      421 gcgatgcgcc atcaagcgaa gcaccgacgg cgatcccgca tagcgtttcc cgcctacgac
      481 ctcgtcgctg ctgctatcgt agacaaatcc ctccattgtc gcgtcggctt cttttccggt
      541 ttccttgaga ctcgccttcc cccacacgag cagagacact ccattgtcag ccgcctgttt
      601 aaagatggcc gtcgccccgt tgctgacttc ccgaaccttg atcccaagac tgggcaagtc
      661 gaccagatcg aacaccaagg accgtttcac atctttcttg agcacctctt caatccgtcc
      721 ccccagccaa tccggcccac cggtattttg aattcccacc acacccagtg aaatcttttg
      781 gaaatcagga cgcgtggctt cgaggaacac atctgcagcc ccggagtcga gaatcccgac
      841 ggctcctgca agcacgagca acccgccgag tatggcccct atgaatgtcg tctgcgtcat
      901 ccggccggct ctcctactct gaacaccatt tcaacgtcct tatacccgtc agtcatgtcg
      961 gatggaaacg gcggaagctg acgcggtcga aggaccgccc gctggccggc catgtcgtaa
     1021 taattattgc cggaagtctt ctgaatcgtc acgtctttga tcattccgtc ccgttgcaag
     1081 cgaaacttga tggtcacggt gtaggtctgc cccgagacat cgatcggagg cggctcccaa
     1141 tggctgctga tcacagcctg tacccgtgcc caatagggat tggagccgga tgagccggaa
     1201 attttgagca tagtctccgg tgtctgcgcc gtcgggatcc tgacttcttg ttgcggagcc
     1261 ggcttcgtcg gctgatcggg cgctctgact tctttcggaa tctctaattt gggcgccgga
     1321 gtgaattgtt ttaccttctt cagctcctcc tcgagctccc gatccacatc atcgctgagc
     1381 tttgatgttc gagaaaccgt ggcagccggt tcttggatcg gatccggaat gcgagggagg
     1441 tctggcactt tcaccttggc ttcccgctga gggagtgcca ctgatttcgc aggagccagg
     1501 tcgccgaact tgggagcatc cggcggcagt tgaaggtccc gcagcacgtc acgcgccaca
     1561 tgtcccgccg gaggtaccgg tgcgcgggcc ggagccgtgt ctttgaccac cggggcagga
     1621 agagatttcg attccggcgg cttgggctga accacgggtt tcaccggttc aggctgcttc
     1681 actggaacgg gagtcgacat gctgacgagc gaaacttcca ccgcagcgag aggccgctcg
     1741 ccgcgctgcg gcaaccgaag gacggtgacg accagcagga tgatgagatg cgcgacaaac
     1801 gagagaagga gcgctcgtcg gaggcggtga aagaaccgcg cctgctcttc gtgatcgaac
     1861 cacagcaccg attggacgta tgcctgagag gccataagac gcgtgttctc tcttcgactc
     1921 ggcccgctaa ctctttcgag acggtgtatg ggtcaactgg ggagcactga cacgttcggc
     1981 accaaccggt tcggtcacca tgccgatctt ttcgatgccg gccctcttca ctccatccat
     2041 aacctgcacg acaacaccgt agggcacctc acgatcggcg cgtaagtaca gcgagacatc
     2101 ccgatgctgc agcttcagca gtttcagttt ctgctccagt tgcgccacac ccaccgggtc
     2161 cttgtctaaa tacagccgtt gatccttctc gatcgtgagg accgtccgca tctcgggctt
     2221 gatcgtattg gtcgcagatt tcggcaaatt gatatccatg ccgcggtaca acatcggcgc
     2281 ggtgaccatg aaaatcacga ggagcacgag cacgacatcg accagcggaa tgacgttgat
     2341 ctccgccaag aatcgtcgtt gtctggattc gaacgccgtc atgcctgcat caccaccgga
     2401 gctggtttcg ctttagtagt ggacgccggt agccaagcca ggagttcgat ggtgaccgta
     2461 tccatacgcg cggcagttcg tcgaatcctg gtgaggaagt agttgtacgc gatgacggcg
     2521 ggaatcgcgg tgaataaccc agccgccgtg gcgatcaatg cctctgagac accgggagcg
     2581 accgcagcga tactcgccgt cccttgcgca ccgatctcac ggaaggcatc gatgatcccc
     2641 aagacggttc ccagcagtcc gacaaacggc gtgatgttcc cggtcgtcgc gagaaaagga
     2701 agataggctt cgagccgtga gagctggcta tgggagagat gcgccgccgt ccgctcgatg
     2761 agatgccgat caattggtgc ggacccggtc ccgtccttgg cgtcgacgcc gccaatcccc
     2821 atgcgttcgg ccgttccttc aaacaaactt ccgcagggac tctcctccat ctttctgatc
     2881 tggcgatata cgtcgccgag atctttggac ttggccaaga gggccataaa ccgctggtcc
     2941 ccgcggtccg ccgcgcgaaa cacgcgccac ttgaagagaa tgaccgccca ggagatcacc
     3001 gagaaaagaa acagcagcac gagtacgctc ttcgacacca gaccgagcga cccgaccagt
     3061 tctacggtac tagtttgaaa catgccccta cgacctttcc cgcataagcg gataatgtgt
     3121 attgctgcat agggagtcta gcaaacattg aggaacaggg aggggccgac agaagttgat
     3181 ccaccctcca tatgttagct tggctctgga ggctgccacg ccaaccgcta cgactgctgg
     3241 ccggtcctct tgtacgtccc tcacgaaagg gaataccacc ccttttagta cgacgtgttg
     3301 tgggcggtga gcccacaaca cgtccattga gataaaagtg gcggggccga cgggatttga
     3361 acccgcgacc tccagattga caatctggcg tcctaaacca ggctgaacga cggcccctcg
     3421 tgactctacc tatagcggaa attcggagcg acaagactat aactagcggg tggtctcgtc
     3481 tgaaattttc aatccttctt tacaatctct ggcctgttcc gaacaaccta gaccctgcgg
     3541 ctggtaggcg gaacagggat cgaacctgtg acctctgcct tgtaagggca gcgctcttcc
     3601 aactgagcta tccgcccgat tttcttagca accttggcgg atgctaccaa gtccaccgac
     3661 ccttgtcaat ccaatctcgc acccttcttg cagaatatgg aacatgtgac cagtttaccg
     3721 tggactcccg acaccagatc tcttgcccac tccgccgcga cgatgcttgc gcgggaaaaa
     3781 tcgatcatgc acgtgcttga gtcgttctcg ctcgacatgc gtatagattt gggtcgtggc
     3841 gatgctcgcg tgtccgagca tggcctggac ggagcggaga tcggcgcccc cctcgagcag
     3901 atgcgtggcg aacgaatgcc gcagcatgtg tggtgaaatc actttcgtga tcccggcacg
     3961 ccgtacgcgt cggcgcagca atttccaaaa agcctgcctt gtcatgggag tcccgcggct
     4021 ggtcacgaag agaaatctcg atgtccgttg cttcaacagc cgtggacgca cctcatggag
     4081 gtagtgtcgc actgtttctc gcgcttgatc attcatcggc acgacgcgct ctttcgaccc
     4141 cttgccggtg acaaaaagat agccgacatc gagattgatc tttgcgactt ccaccgaaac
     4201 aagttccgac acccgcaatc ccgtcgcata gagcagctcc accatcgccc ggtcgcgatg
     4261 gtcctctcgc gtgtccaagg cgggcagatc cagcaacgtc gttacttcgt gctgagacaa
     4321 ggtcctcggc agtttgatgc cgcgacgcct ggacgccagc ctgacggcag gattttcatc
     4381 gatcacccgc tcctgcctga gaaaccggag ccagccgcga acggcggata ggcagcgggc
     4441 tgaagaagtg tgcgacaatc gagcctctct caatgagcga agaaatcccg tgaccgtgcc
     4501 ggctgtcatc tgcgcaggta acgacacacc aacccgatgt gcataggttt gaaacttgag
     4561 cagatctcgg cgataggcct ccagcgtgtt aatcgacagc cctccctcga tgcggaggtg
     4621 agtcaagtac cgttcaacca tcggatgcag caacatgttt cgatgatagc gaatcactgc
     4681 gatgaagaca acgaacggcg cggagattgc cttgacaccc cacagggttt ccgatagtag
     4741 tcacgtgaac acgaaattgc gtaaccttcc actggaaccc atgatccatc gcatcatcgc
     4801 ttcgtatggc ttgtctgatc gtccatgtcg tgcgctgttg agtctgctcg ccgtcctcgc
     4861 tttcatctca gcagagatcg acccatccgc cggatggagg gagagtcgat cggttgacgc
     4921 cgcaacggcg aaaggcacac cggaatcgca tcccgcgctg acccaagcca aacgtctgct
     4981 cgacgccggg caggcggaag aagcgaccac tatcctgcgt cgcttcttgg gcacgtcacc
     5041 caagcctgag ttcttggatg acacctatct cctgctcgga gccgccttgt acgacacgag
     5101 agaatatccg gaagccttga agtacctaca gcaactgcag cttgagttcc ccgattcaga
     5161 agtctcggac cggggcaagt tgatgctggc ccgcgtccat gcggcgatgg gaaacgtcga
     5221 ccttgccttg ccgatccttg tcaatatccg cacgctctcg aaagacgaca cggccaaact
     5281 ggaggcgatg cacctgagcg gcgatctcta tgtgaaaaaa aaggaccctg tgcgggctat
     5341 tcaagcctgg ttggatgaaa tggctctcgc tccagacgat caagccgagg acatccatgc
     5401 tcgtatcagc gaactcatca atggacaacc ggacaggaaa accctcgaac ggatccaaga
     5461 actctattcc cgaacgtttc ccggcgacct cgcctcggtt cgtctgatcg agttctatgt
     5521 cgcgcgcggt gaagaccatc aagcgacccg tcagattcag cagttcttga atcgcttccc
     5581 gacccatccc tacgccgcga aggcctcgga gctgctcggc acgttacaaa ccaaactcaa
     5641 atcttctcaa ttcgtgctcg ccgccgtgct gccgctgtcc ggaaaggccg cgcccttttc
     5701 caacgacgtc ttgtcaggta ttcaactggc cgttgaactc agcaaggatc gaaccaccgg
     5761 ttcatcaatc gggttgatcg tgaaagacta cgaatccgaa cgccccacgt ttctcgatga
     5821 cctgtctcgg ttgttgatag acgatcaccc gatcgccgtc atcggtccgc tcctgtcgaa
     5881 gagtttgccg gtcatggctg aattggcgga acggaccaaa actccactca ttacgccgac
     5941 tgccacaatc ccaaacgttc gccgattggg cggctatgtc ttcagcacgg cgctcaccta
     6001 tagcatgcag gcgaggagga tcgcgtccta tgccgccctc gagcagggat accgccgctt
     6061 ttgtatcctg tatccggaca cgacctacgg tcgtgaactt gcacgactat ttgcgctgga
     6121 gatccaacaa cacgacggcg aaatcattgc gatggagtcg tataaggaag gcgagaccga
     6181 cttcaacgcg caactcaaac ggatgaaggc tgaggatttg aagaagtatg ggctctccgt
     6241 gccggtggat ccgacgaaga tgaccgggag acaaacgaaa ctcgacaaga aagtgctcta
     6301 tacgcccgga ttcgatgcga tcttcgtccc gggacgcgcc caggaagccg gtctcatcgc
     6361 cgcgcaactg aactttcacg ac
//
LOCUS       DATDNW010000238         6308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_451151, whole genome shotgun sequence.
ACCESSION   DATDNW010000238 DATDNW010000000
VERSION     DATDNW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451151"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VI359_05855"
     CDS             complement(<1..540)
                     /locus_tag="VI359_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY6973809.1"
                     /translation="MSSELDRQALIDIFVTEASEAMAALTSALNPSDGSIPQPQQLQE
                     QYVWAHKIRGASALYGFNGLATLGALLESTLEQASSIDDGIWPKVVGVLRGMVEAFQT
                     QLEGIRQGEGEDTAACEHWKSEVDGLLPESSETAPPAPSSSRLSQDYLVPTIDADVLS
                     YFSPEADEYLHAMELFLQRL"
     gene            complement(590..2488)
                     /locus_tag="VI359_05860"
     CDS             complement(590..2488)
                     /locus_tag="VI359_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY6973810.1"
                     /translation="MAKADAKIDSKGSHFKGRFYNLNTRTKLISSFGVVSLIILIMSS
                     VGVLTLRQLSSQSRTVYADYTVPLANFADMGTALTTHHQILTSMATITRQADFAAEVA
                     RLPQYRAKARKVLADYEATAFRVSRSGRDEAKDYALLKPAIQQYFQEADGALSAMADS
                     FDKSLTSEQAENMRGLGVLALTVNLQPTFDKAVTRHREQVLDMQEVAKDLNDDAQAIS
                     ENGTLILIGGGFIAVVLGLGIGYWMAGFFSKGLTHIAQVASQAAAGNYQARAKVSTKD
                     ELGQMATSFNAMLDRITALVTSEEERDLMQKRLMNFLVLVSDVGKGDLTKRGEVTADM
                     FGNLADGFNLMIQRFGQLLKQVREAAERVNKSAGTLRESAGQMAGTAKHQADESVKTL
                     GAVEQLATSMRQVAETAGASSESAKQVLQATERGRVAVQETVQDMQSIRAAVQRMSKQ
                     VKALGDRSLEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAE
                     SSTQATREIADLVKVIQTETQDAVVAMEHETQAVEAGSASALRTGDVFNEISTIAQRS
                     SELAQIIASSAANQTASTDQVGRSIKDFTGGAVATQKATDAARVTVEDMAKLAEGLTS
                     SVSQFKLA"
     gene            complement(2594..3088)
                     /locus_tag="VI359_05865"
     CDS             complement(2594..3088)
                     /locus_tag="VI359_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY6973811.1"
                     /translation="MEHQQEYTPQGLLETDHQSAGMNASRMCLISLSGELFAMDLGHV
                     TEVFEVDSITPVPGAPSTLIGVANLRGTIIPLADLRPSLGLSATDSSLKYAVVVRHGS
                     QQIGILVDTVPEIRTVRQDEFLAAPTQGSANAQPLVSALLRVEERMGGVMEVPRLLTT
                     VGLG"
     gene            complement(3134..3496)
                     /locus_tag="VI359_05870"
     CDS             complement(3134..3496)
                     /locus_tag="VI359_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973812.1"
                     /translation="MSKILIVDDSYAELQMIEGYLKGASHTVVSFPNTEKLEDKVISE
                     KPDLIVMDVVMPGRNGFQACRDLKNDDRTKNIPIVLCTSKGNESDKFWGQQQGANGYV
                     TKPFKAEDLLAAVKRALG"
     gene            complement(3555..4286)
                     /locus_tag="VI359_05875"
     CDS             complement(3555..4286)
                     /locus_tag="VI359_05875"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6973813.1"
                     /translation="MRKIAMGLIGMGMVALSFTWTSSATAGDEAETAELLITLLKAGR
                     GVVSENQGLINDASKAHKGFTDDAFAQQALEKFKAKTKIDLAKAGNLPQGPMLKALLD
                     SEKEVVAEAQMVINKQGIGFKGFVPAVFARKTGERFYQKTGVKLRLTGLDYRNPNNKP
                     DDFEQEVLRMFADPRHPKGQQYVKTTMLAGKPVLRVMDPEYAGPTCLSCHGSPKGERD
                     ITGMKKEGWKEGDLAGAISLTLPIK"
     gene            complement(5106..5864)
                     /locus_tag="VI359_05880"
     CDS             complement(5106..5864)
                     /locus_tag="VI359_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEY6973814.1"
                     /translation="MDLRTHYRVIANTLVIDLRRPSKVLSSAPQGGGLRSVRYILNHQ
                     IKVPAGSNCTTAAVKKTRQPEWGDPARYLRRLATAVGVDNNCVALMTAVPMKQLVIGH
                     EESQGIWVECFATVGVTNAVRAGEWPGVESDPKRDACLGTINLILVTNTTLSSAAMVG
                     AVQVATESKTGILRDHAVPSWTGVAGATGTGTDAVVVACALRGIGARLSYSGTHTIIG
                     AMIGRVVGECVTKGLARAERWITHAASQSRSVST"
BASE COUNT         1355 a         1860 c         1579 g         1514 t
ORIGIN      
        1 aagtctttga agaaacaatt ccatggcatg cagatactca tccgcctccg gagagaaata
       61 cgataggacg tccgcatcga ttgtggggac taagtaatct tgcgacagcc gactcgacga
      121 tggtgccggc ggcgcagttt cggaagactc tgggagaaga ccgtcaacct cggatttcca
      181 atgttcgcag gcggccgtat cttcgccttc tccttgtcta atcccctcca actgagtctg
      241 gaacgcttcc accatcccgc gcaagactcc cacgactttc ggccatatcc cgtcgtcaat
      301 ggaagaagct tgttcgaggg tgctctcgag cagcgcgccc aacgtggcaa gtccgttgaa
      361 cccgtacagt gccgaggccc cgcgaatttt gtgggcccac acatactgtt cttggagttg
      421 ctggggttgc ggaatcgaac cgtcgctcgg attcagggcg gacgtgagtg ccgccattgc
      481 ttccgaggct tcggtgacaa agatgtcgat gagggcctgt cggtccagct cagaactcat
      541 gccgtccttt cttacccgga acgccgaatc ggcgccccgg ctcaccggcc taggctagtt
      601 tgaactgcga gaccgaggac gtcagacctt cagcgagctt ggccatgtct tcgacagtca
      661 ctcgggcggc gtccgtcgct ttctgcgtgg ctaccgcacc gccggtgaaa tccttaattg
      721 aacggcctac ttgatccgtc gatgccgtct gatttgctgc ggaactcgca atgatttggg
      781 ccagctcgga tgatcgttgc gcgatggttg aaatttcatt aaacacgtcg ccggtacgga
      841 gcgcagacgc cgaacctgct tccacggcct gagtttcatg ctccatcgct acgaccgcat
      901 cctgcgtttc agtctgaatg actttcacaa gatcggcgat ttcacgtgtc gcctgcgtcg
      961 agctttcggc cagttttcta acctggtcgg caacgactgc gaaacgtgct ccggcttcgc
     1021 cggcgccggc cgcctcaatg gcggcgttca gcgcgagcag gttagtctga ttcgcaatgt
     1081 cgcgaatcgt ggacacaatc tgcgagattt ccaatgatcg atcgccgagt gctttcacct
     1141 gcttcgacat ccgttgcacc gcggctcgga tgctctgcat gtcttgcacg gtttcttgca
     1201 ccgccacacg gccgcgctca gtagcttgca acacttgctt ggcggattct gacgacgccc
     1261 ctgcggtctc cgccacctga cgcatcgatg tcgcgagttg ttcgaccgcg ccgagtgtct
     1321 taaccgattc atcggcttgg tgttttgcgg tacctgccat ctgtcccgcg ctttctcgca
     1381 gtgtcccggc ggatttattg actctttccg cagcctctcg gacctgtttg agcaattggc
     1441 cgaaccgctg aatcatgagg ttgaatccgt ctgctaagtt cccgaacata tcagccgtaa
     1501 cttcaccgcg tttcgtcaag tcccccttac cgacatccga caccaagacc aggaaattca
     1561 tgaggcgctt ttgcatgagg tcgcgttctt cttccgaggt cacaagcgca gtaatacgat
     1621 ccaacatggc attgaacgat gtagccatct gaccaagctc gtccttcgtg gaaactttcg
     1681 cacgggcctg gtagttgccg gccgcggctt gcgatgcgac ctgagcgata tgtgtgagac
     1741 cctttgaaaa gaagccggcc atccaataac cgatgccgag tccgagcacc acggcgatga
     1801 accctccgcc aatgagaatg agcgttccat tttcagaaat cgcttgtgca tcgtcgttca
     1861 gatctttggc gacttcctgc atgtccaaga cctgttcacg gtgacgcgtg acggctttgt
     1921 cgaatgtcgg ctgcaggttc actgtcaacg ccaacacacc aaggcctcgc atattctcgg
     1981 cctgctccga cgtcaatgat ttgtcaaaac tgtcggccat ggcgcttaag gcaccatcgg
     2041 cctcctgaaa gtactgctga atggccggct tgagaagggc atagtccttc gcttcatccc
     2101 gacccgagcg ggagactctg aacgcagtcg cttcatagtc cgcgagaacc ttcctcgctt
     2161 ttgcccgata ctgcggcaat ctggcgactt cggcggcgaa gtctgcttgt cgcgtgatgg
     2221 tggccatgct ggtcaagatc tggtgatggg tcgtcagtgc agtccccata tcggcgaaat
     2281 tcgccagcgg gaccgtatag tctgcgtaaa ccgttcgcga ctgactactc aactgtcgaa
     2341 gtgtcagcac accgacgctc gacatgatga ggataatgag actgacgaca ccgaagctcg
     2401 aaatcagctt cgttctggta ttgagattat agaatctccc cttgaagtgt gatcccttcg
     2461 aatcgatctt cgcatctgcc ttagccatcg cgctcctcct tctcccgagg tatgtctcgc
     2521 tgaagcagcg agcaaccgga tcattctgga tctattgtgc gatcgacacg ctcaacaatt
     2581 ggtgttactg cccttatccc aaccccactg tggtcaataa gcgtgggact tccatcaccc
     2641 cacccattcg ttcttcgaca cgcaacagag cggagaccaa cggctgtgcg ttcgcggatc
     2701 cttgcgtcgg tgcggcaaga aactcatcct gtcggaccgt gcggatctca ggtaccgtat
     2761 ccactagaat gccgatctgc tgacttccat gccgcacgac cacggcatac ttgagggatg
     2821 aatctgtcgc tgacaatccc agcgaagggc gtagatcggc caacggaata atcgttcctc
     2881 gaagattggc aacgccaatc aatgttgagg gagcgccggg aaccggcgta atcgaatcaa
     2941 cctcaaagac ttctgtcaca tggcctaggt ccattgcgaa taattcaccg ctcaggctga
     3001 ttaagcacat acgggaggca ttcatccccg ccgattggtg atcggtttcc aacaaccctt
     3061 gcggagtata ttcctgttga tgctccatca ctctccgatg acagcactct ccaatgacag
     3121 cagtagcatc gccttagcct aaggcgcgct tgacggccgc gagcaaatct tctgccttaa
     3181 acggctttgt cacatagcca tttgcccctt gctgttgccc ccagaacttg tcgctctcgt
     3241 ttcccttcga cgtacagagc acaatcggta tgtttttcgt ccgatcgtcg ttcttaaggt
     3301 cacgacaggc ctggaaaccg ttgcgtcccg gcatcacaac atccatgacg atcagatcgg
     3361 gtttttcgga gatgacctta tcctcgagtt tctccgtatt gggaaatgaa accactgtgt
     3421 gactagctcc tttcagatag ccctcgatca tctgcaattc cgcatacgaa tcgtctacga
     3481 ttagaatctt gctcatgtct ggtaccctcc tgatgattga tgttgtgatg aagctctgta
     3541 atcccgtgtt ctgtttattt gatgggaagc gtcagactga tggctccagc cagatcccct
     3601 tccttccatc cttccttttt catacccgta atgtcgcgtt caccctttgg cgacccatga
     3661 cacgataggc acgtgggtcc ggcatattct ggatccatca cacgcaacac cggcttccca
     3721 gctagcatcg tggtcttcac atactgttga cccttgggat gccgaggatc ggcgaacatg
     3781 cgcaagactt cctgttcaaa gtcatccggc ttgttgttag ggttccgata gtccaacccg
     3841 gtgagtcgca gtttcacgcc cgttttctga tagaatcgtt ccccagtttt tctcgcgaac
     3901 accgcgggaa cgaacccctt gaacccgatg ccctgcttgt tgattaccat ttgagcttcg
     3961 gctacgactt ctttctccga gtcgagaaga gccttcagca ttggaccttg cgggagattg
     4021 cccgccttgg cgagatcaat cttcgttttt gccttgaact tctcgagagc ctgctgcgca
     4081 aaggcatcgt cggtgaaccc tttatgagct ttcgaagcat cgttaataag cccctgattt
     4141 tccgatacga ccccgcgccc ggctttcagc agtgtaatca acagttcggc ggtttccgcc
     4201 tcgtctccag ccgttgccga cgatgtccac gtaaacgaaa gcgctaccat tcccatcccg
     4261 atcaggccca tcgcaatttt gcgcataacc cttcctcctt cgtcggatca acagccgtga
     4321 tcaggtcacg acattgttgt gtaaacctga gtgtagcgtt tcattctcta ttggcaagtg
     4381 aatcaataga atctgcatcc ctctccctat ggggcactga cgtgccgctg acaacatcgg
     4441 cgcacatacg gtcaccgcac tcgcacacac ggcaccgcag gttccgcgca caactccagc
     4501 aacttacgtg ccgacactac gtactgccca caagaatgtt ttctcaccca tacgcttgac
     4561 ctgcacaaat gctgtcaaat caacaacaac tgtcgtaaac gctggcgact gttacaaccc
     4621 tgaccatcag tttcccattt gtgcacagtt gtgcatcaca gccgaatttt ggacttcgaa
     4681 cattgagcga ctgactgagg tttatctctg gacgagaccc atgcatgcag gagagacaca
     4741 gggatgcgca tgattgttca atacaggagc gggatttatg aactgggaga cagcgtcaca
     4801 ggagatccct gagaagcagc atcatattca agtggatgtc gatatggaga tctcatgggc
     4861 caaatctgcc tgacaattgt ttcgtaatcc cttccgatac atgcatcgag agggacgata
     4921 acccttttct cactcgaaca cggttcaacc acatcgaccg aaatccagga tcgtcacatg
     4981 gctcatatat atacgtcccc cacacgagac catcatggcg aacagcgcca tcatgatcac
     5041 catccgctcg ccatctttct ggagaattcc gtgaccgcta gctgtgagga tgcttcattc
     5101 gtttcttacg ttgaaaccga ccgtgattgg ctcgcagcgt gggtaatcca gcgttccgca
     5161 cgtgctaaac ctttcgtgac acactcgccc acaacccgac caatcatggc gccgatgatg
     5221 gtatgcgtcc cactatagga cagccgagct cctatccctc gaagcgcaca tgcaacgacc
     5281 actgcgtctg tgccggttcc tgttgctccg gccacaccag tccagcttgg aacggcatgg
     5341 tcacgtaaaa ttccggtctt gctttcagtt gccacctgca ccgcgccaac catggccgcg
     5401 ctggaaagag tagtgttcgt gaccagaatg agattaatcg tcccgaggca agcgtccctc
     5461 tttggatcag attcaacgcc tggccattcc cctgctcgaa cggcattggt gactccgacc
     5521 gtcgcgaaac actcgaccca gatcccctga gattcctcat gaccgatcac gagttgtttc
     5581 atgggaacgg cagtcatcag agcgacgcaa ttgttgtcga cacccacggc agtcgccagt
     5641 cggcgaagat accgcgcagg atctccccat tcgggttgcc gcgttttctt aaccgcggct
     5701 gtcgtacagt tcgagccagc aggtaccttg atctggtgat tgagaatgta gcgaaccgat
     5761 cgaagtcctc ccccttgcgg agcagacgag agtactttcg aaggacgacg aagatcgatc
     5821 acgagcgtat ttgcgattac ccggtagtgc gtacgcaggt ccacgagatt ctgtcgcctc
     5881 atctcttact gatctgcgag tacacgagag agtgccttca tcaggcgatc gttctgttgt
     5941 ctcgttctca ctgctagtct gacggaacga gaatgcagac ccggtacagc ggagcagtca
     6001 cgaattaaga gaccatattt cctgagccta gcagttattg ttctggcatg gcacccggcg
     6061 ggcagttcca tgaggatgaa gtttgcgaat gaaggaaaca caacacaacc cggcaatcct
     6121 gcgagcaacg tcccgaatcg cgagcgttcc ttcgctataa atgaatgact tcgtcgggta
     6181 tggccggcgt ctttcagtgc cgccacagct gccacttgcg cgactgcatt gacagaccaa
     6241 gggggttgtt gtcggcgcag ttgacgagag acaagactat gggcaaccgc atatcccact
     6301 cgcaggcc
//
LOCUS       DATDNW010000239         4059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_168306, whole genome shotgun sequence.
ACCESSION   DATDNW010000239 DATDNW010000000
VERSION     DATDNW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168306"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VI359_05885"
     CDS             <1..1806
                     /locus_tag="VI359_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973815.1"
                     /translation="DTVPHTVNIIEAGNPPGQPVEVALEAGKTVSFQMPEKILNAPNK
                     YINGGTHTTGSGLNFRAAPFAQRLANDPDTSKLFSSAIHGDPGTPLLRAYTGDTMVFR
                     LLHQLMNESHVWTISGHTFLTERYAADANRKNSIHVGIAERYDLVTKAGGFQGMPGDY
                     IHFNGRSSHFAEGGWGIVRVLDKETADLKPLPKGTNPLGITAAPSSVCPADAPVKSFN
                     VVALDRPMKLNPKAPDAIEVDFERKIEMTMPEGKIFALEEEATTVASGATPNPLTLRA
                     NLGDCIKVNLKNKMKASRASFFAPGLSFNPKDSQGLNVGNNPGDQTIAPGESRTYTYY
                     AHPANKETTSLVWDGGNIVVNPRNGLYGAVIVGPKGSQYRDPVTGADISQKNSWRADV
                     IVDASLPENVGKRNYRDVALFFQDEDNIIGTAFMPYVQNVAGLTSVNYRSEPYKFREE
                     QGCSLGKIFQPCVVDKPEDPVTPLIEAHAGDPIRIHVIGANSEQNGMFGVEGHEWPIE
                     PYMPGADMISVVEYAGSEILDVFIRGGAGGPYRQVGDFVWSNARLPYTQSGQWGYLRV
                     LPTGDSRLQPLGIQGMGAKRADVQPEPQAIPTAMK"
     gene            1974..2906
                     /locus_tag="VI359_05890"
     CDS             1974..2906
                     /locus_tag="VI359_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY6973816.1"
                     /translation="MKIQSTILSMLIVGWLAGTAWAYEEITVADGGSLSGTVTLLGEV
                     PKPKGYNLVTLPDPYYCGRISDGQGWRILQPFQVGPRGEFRDVVVYLEGIDKGKPFEE
                     GGVPQIEAKDCLFLPFTTVVRDDQTVTVVNMDPVMHDIQAYETSHLGPRVLFNVPLPM
                     NPRHPRNLKDRSDAAMYHKHMAGAPMKQLVNLSKGRRTFVMQCGFHAYMESWGVAVTN
                     PYFAKTDEQGRFTMTDVPPGTYKLVVWHPYVRTEVEQTVTIGPKGTEEANIAIPAPTG
                     RLYANQVLEHAYVRYNVTEETKKEIDPMIHKQDH"
     gene            2969..3928
                     /locus_tag="VI359_05895"
     CDS             2969..3928
                     /locus_tag="VI359_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY6973817.1"
                     /translation="MAESSVVPMARATTFKLAIAATLLAFVPIGLAYANHESSKQPPL
                     WTPLDEAERLAMIDVPGGMVRVPAGDFLMGSDPRKDHAAGPQEVPQHRVYLDAFEIDR
                     YEVSNVQYLRFVLATGTDWPKFWRESPFPEKAALHPVINVSWNEADAYCRWAGKRLPT
                     EAEWEKAARGADGRIFPWGDEPAGWIKSNIAHSGSKRGFKYPPLANVNRYDRGVSPYG
                     IYQMAGNVSEWVADWFDPEYYRKGDNENPKGPESGDIKVFRGGSWNEDPEVARSAGRN
                     GGEPNRRSYLTGFRCAQSDLGTRHEARGEREEIVSPAPLASRR"
BASE COUNT          906 a         1082 c         1299 g          772 t
ORIGIN      
        1 gacacggtgc cgcatacggt gaacatcatc gaagccggca atcctccggg acagccggtt
       61 gaagtggcgt tggaagcggg caagacggtg tcgttccaga tgccggaaaa gatcctgaac
      121 gcaccgaata agtacatcaa cggcgggacg catacgaccg gcagcggcct caatttccgc
      181 gcggcaccgt ttgcacaacg cttggccaac gatccggata cgtcgaagtt gttcagcagt
      241 gccatccatg gagacccggg tacgccgttg ctccgtgcgt acacggggga caccatggtg
      301 ttccgcctgt tgcaccagct gatgaacgaa tcgcacgtgt ggacgatctc cggccatacc
      361 ttcctcacgg aacggtatgc ggcggatgcc aatcggaaga actcgattca cgtcgggatc
      421 gccgagcggt atgacctcgt caccaaagcg ggtggattcc agggcatgcc gggcgattac
      481 attcacttca acggccgaag ctcgcatttc gccgaaggtg gatggggtat cgtgcgcgtg
      541 ctcgataagg aaacggcgga tctgaagccg ctcccgaaag gaaccaatcc gctcgggatt
      601 actgccgccc cgagttccgt ctgtccggcc gatgccccgg tgaagagttt caacgtcgtg
      661 gcgttggatc gcccgatgaa gctcaatccg aaggcgccgg acgccatcga ggtagacttc
      721 gagcggaaga tcgaaatgac catgccggaa ggcaagatct ttgcgctcga ggaagaagcc
      781 acgacggtgg cgagcggggc aacaccaaat ccgctgacgc tgcgtgcgaa tctgggtgat
      841 tgtatcaagg tcaatctgaa gaacaagatg aaggcgagcc gggcatcctt ctttgcgccg
      901 ggcctgtcct tcaatccgaa agacagtcaa ggtctgaacg tcgggaataa ccccggcgat
      961 cagacgattg ctccgggcga gagccggacc tacacctact acgcgcatcc agcgaacaag
     1021 gagacgacgt cgttggtgtg ggatggcggc aacatcgtcg tcaatccacg gaacggattg
     1081 tacggagccg tgatcgtcgg cccgaaggga tcgcagtatc gcgatcctgt gacgggagcc
     1141 gatatttcgc agaagaattc gtggcgtgct gacgtcattg ttgatgccag cttgccggag
     1201 aacgttggga agcgaaacta ccgtgatgtc gctctcttct tccaggatga ggacaacatc
     1261 atcgggaccg cgttcatgcc ctatgtgcag aacgtggccg gtttgacgtc ggtgaactat
     1321 cgttccgaac catacaagtt ccgtgaagag caaggatgct cgttgggcaa gatcttccag
     1381 ccctgcgtgg tggacaagcc cgaagatccg gtgacgccgt tgatcgaggc gcatgccggt
     1441 gacccgattc gcatccatgt gatcggggcg aacagcgagc agaacgggat gttcggtgtc
     1501 gaaggccacg agtggccaat cgagccctac atgcctggtg cagacatgat cagtgtggtg
     1561 gagtatgccg ggtccgagat cctcgatgtc ttcatccgtg gcggggcggg cggtccgtat
     1621 cgccaggtcg gagacttcgt ctggtcgaat gcgcggctgc cctacacgca gtcagggcaa
     1681 tggggttacc tccgtgtgtt gccaactggc gattcgcggc tccaaccgct cggaatccag
     1741 gggatgggtg ccaagcgtgc ggatgtgcag ccggagcctc aagctattcc gacagcgatg
     1801 aagtagtcga cagcggaata gttgtcgtcg atgcggggga gctgcgcgca atgtgtagct
     1861 cccccgtgtt ctaagaagtg gggattcacg agtgctgagg ctgggtctcg acgcatgaat
     1921 ttccaacctt ggtattcaga acttaacggt gaagaggagg aggccggaac aatatgaaga
     1981 tacagtcgac cattctgagt atgctgatcg tcgggtggct tgccgggact gcctgggcct
     2041 atgaggagat cacggtcgcg gacggtggca gtctctccgg gactgtcacg ctgctgggtg
     2101 aagtcccgaa gccaaagggg tacaacctcg tgaccttgcc tgacccctat tattgcgggc
     2161 gcatttccga tgggcaaggc tggcgtatcc tgcaaccgtt ccaggtcggg cctcgagggg
     2221 agtttcgcga cgtggtggtc tatctggagg ggatcgacaa aggcaaaccc ttcgaggaag
     2281 ggggcgtgcc gcaaatcgaa gcgaaagact gcctcttcct cccgttcacg acagtcgtgc
     2341 gggacgatca aacggtgacg gtggtcaata tggaccccgt catgcatgac attcaggcct
     2401 atgaaacctc gcacttgggt ccgcgcgtgc tgttcaacgt gccactgccg atgaatcccc
     2461 ggcacccgcg caacttgaag gatcgcagcg acgccgcgat gtaccacaag cacatggccg
     2521 gcgcgccgat gaagcaattg gtcaatctca gcaagggtcg ccggaccttc gtcatgcaat
     2581 gcggcttcca tgcctacatg gagagctggg gcgtggccgt caccaacccg tattttgcca
     2641 agacggacga gcagggccgg ttcacgatga ctgatgtgcc gccaggcacc tataaactgg
     2701 tcgtctggca tccctatgtt cgtaccgagg tagagcaaac cgttaccatc ggaccgaagg
     2761 ggacggagga ggccaatatc gcgataccgg ctccgaccgg acggctctat gccaaccaag
     2821 tgctggagca tgcctatgtt cgctacaacg tgactgagga aacgaagaaa gaaatcgatc
     2881 cgatgatcca taagcaggat cactgaacag ggagcgagag cactcagcta tcagccgtca
     2941 ggttccgacg tcagaaagct ggcagctgat ggctgaaagc tcggtggttc ccatggccag
     3001 agcgaccaca ttcaaattgg cgattgcagc gacgctactg gcgttcgtcc cgatagggct
     3061 tgcatatgcc aaccatgaat cgtccaagca acctccgtta tggactccgc tcgatgaagc
     3121 cgagcggctg gcgatgattg atgtaccagg agggatggtg cgcgtgccgg ccggagactt
     3181 cttgatgggg agcgatccgc gaaaagatca cgccgccggt ccgcaagaag tcccgcaaca
     3241 tcgggtctat ctcgatgcct tcgaaatcga tcgttacgaa gtgtcgaatg tacagtatct
     3301 gcgttttgtg cttgcgaccg gaacagattg gccgaaattt tggcgggaga gcccgtttcc
     3361 tgagaaagcc gcgctacatc cggtaatcaa cgtcagttgg aacgaagcgg atgcctattg
     3421 tcgatgggcg ggcaagcgat tgcccacgga agcggaatgg gaaaaagccg cgcggggcgc
     3481 agatggacga atctttccct ggggcgacga accagctgga tggatcaaga gcaacatcgc
     3541 gcactcgggg tcaaaacgcg ggttcaagta tccacccctc gccaacgtga atcgctatga
     3601 ccgtggtgtc agcccgtatg ggatttatca gatggcgggg aacgtcagcg agtgggtggc
     3661 ggattggttc gacccggagt actaccggaa gggtgacaat gagaatccta aggggcctga
     3721 gtcgggggac atcaaagtct tccgcggagg ttcatggaat gaagatccgg aagtggctcg
     3781 ctctgccgga cggaacggcg gtgaaccaaa caggcggagc tacttgacgg gatttcggtg
     3841 cgcacaatcg gatttgggca cgaggcatga ggccagaggc gagagggaag agattgtttc
     3901 ccccgcaccc cttgcctcgc gtcgctagcc gaggcaacca ggaggaagcc gaaacatgga
     3961 ggggaaagag gcggagatat tccggaagtg ggagtggggc atcaatgccg tcttggtcat
     4021 cgccgtgctg ctaatcatgg ctcgcgtggc ctgggggct
//
LOCUS       DATDNW010000240         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_795581, whole genome shotgun sequence.
ACCESSION   DATDNW010000240 DATDNW010000000
VERSION     DATDNW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795581"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VI359_05900"
     CDS             <1..586
                     /locus_tag="VI359_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY6973818.1"
                     /translation="AISMFGYGGIVLTMAIESACIPLPSEIIMPFSGYLVATGQFTMV
                     GVTLAGAIGNVVGSVAAYYAGIWGGRPFVERYGPYFLVSRKDLDLADRWFQKYGEAAV
                     FFSRMLPVVRTFISLPAGVARMNFPRFVLFTFIGALPWCYLLAYIGLKMGERWDQLRD
                     YFHQFDIVIGLGLALVVGYFLWSHWPRRQPNLES"
     gene            594..2027
                     /gene="cysS"
                     /locus_tag="VI359_05905"
     CDS             594..2027
                     /gene="cysS"
                     /locus_tag="VI359_05905"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY6973819.1"
                     /translation="MLTIYNTLTGKKEPFQSLVPKTVRMYVCGVTVYDYCHLGHARSA
                     LVFDMIRRYLEYGGYTVEFVKNFTDVDDKIIKRANEQGVSCDAITTKYIQAYYEDMDK
                     LGVRRASKEPKATESMADIIGLIEKLVKQDIAYKVDGDVYFQVKRYPAYGRLSKRKLE
                     DLQAGARVEVDERKRHPMDFALWKSGKPGEPSWDSPWGLGRPGWHIECSAMSMKYLGE
                     TFDIHGGGMDLIFPHHENEIAQSSGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFEKSKWQQEVTGEVLRYFLLGTHYRSPLDFSDQSLHEAKNAVDGFYDLFMRLREP
                     DGKGIGDGELAQTIDRCRLAFRAAMDDDFNTPVAIAELQRLRNDVNKLVVQGLSTEGR
                     KRVREEFSSFGRVLGLFNSDIWHYVLQPESGNLKMSGEEIEIKIAERNEARKQKDFTK
                     ADEIRQFLASHGIIIEDKPDGTSRWKR"
     gene            2050..>2358
                     /locus_tag="VI359_05910"
     CDS             2050..>2358
                     /locus_tag="VI359_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY6973820.1"
                     /translation="MHAVREALRAKTRPLQRVLVLRIDRQFADLVQLAKAQQVPVHIE
                     PQPAFDRLVPHGRHQGVIAFVAAKSYSSTEDILQRAKERGEPPFLVLLDGVEDPHNLG
                     A"
BASE COUNT          542 a          572 c          688 g          556 t
ORIGIN      
        1 ggcgatttca atgttcggct atggcgggat tgtcctgaca atggcgatcg aaagtgcctg
       61 cattcctttg cccagtgaga tcatcatgcc attctccggt tacctagtgg cgaccggaca
      121 atttacgatg gtaggcgtga cactcgccgg cgcgatcggc aatgtcgtgg gatcggtggc
      181 ggcgtactat gccgggatct ggggcggtcg gccgttcgtt gagcggtatg ggccgtattt
      241 cctcgtgtcg cgcaaagacc tcgaccttgc cgatcgctgg tttcagaagt acggagaagc
      301 tgccgtgttc tttagcagga tgttgccggt agtccgtact ttcatctcgc ttccggcggg
      361 agtcgcacgg atgaatttcc cgcggttcgt gctgtttaca tttatcgggg cgttaccctg
      421 gtgttatctc ttggcgtata tcggtctgaa gatgggcgaa cggtgggatc agttgcggga
      481 ctactttcat caattcgaca ttgtgatcgg tctcgggctg gccttggtcg tcggctattt
      541 cttgtggtct cattggccaa ggcgccagcc gaatttagag tcgtaagcac cgcatgttaa
      601 cgatttacaa taccttgact ggcaagaagg aaccctttca gtcgttggtt ccgaagaccg
      661 tgcgtatgta cgtgtgcggt gtcacggtct atgactattg ccatctgggt catgcccgca
      721 gcgccctggt tttcgacatg atccgtcgat acctggagta tggaggttac acggtcgagt
      781 tcgtgaagaa cttcacggac gttgatgaca agatcatcaa acgagccaac gagcaaggag
      841 tttcctgcga cgcgattacg acgaagtata ttcaggccta ttatgaagac atggacaagt
      901 taggtgtccg ccgcgcttcg aaagaaccga aagcgacgga gtccatggcc gacatcatcg
      961 gactgatcga aaaattggta aaacaagata tcgcctataa agtggacggc gatgtgtatt
     1021 ttcaggtgaa aaggtatcct gcctatggcc gcctgtccaa gcgcaaactc gaagaccttc
     1081 aggcgggggc gcgcgtcgag gtcgatgaac gtaagcgtca cccgatggat ttcgcgttat
     1141 ggaagagcgg caagcctggc gagccttcct gggatagccc ttggggactc ggccggccag
     1201 gctggcatat cgaatgctcc gccatgtcca tgaaatacct tggtgagacg ttcgacattc
     1261 atggcggggg gatggacctg attttcccgc atcatgagaa cgaaatcgcg cagtcgtccg
     1321 gcgcgacggg caaggaattc gctcgttact gggttcacaa cggttttgtg cagatcaatc
     1381 aggaaaagat gtcaaagtcg ttagggaatt tctttacgat tcgggagata tttgaaaagt
     1441 ctaaatggca gcaggaagtc acgggagaag tgcttcgata tttcctcctt ggcacccatt
     1501 atcgaagtcc attggatttt tcggaccaga gcctgcacga agccaagaat gcggtagatg
     1561 gattctacga tctcttcatg cggctgcgtg agcccgatgg gaaaggcata ggtgatggcg
     1621 agttggcgca gacgattgac cgatgtcggt tagcgtttcg agcagccatg gacgacgatt
     1681 tcaacacgcc cgtggcgatt gctgagctac aaagactccg aaatgacgtg aacaaattgg
     1741 ttgtgcaggg tctttcgact gagggacgaa agagagtcag agaggaattt agttccttcg
     1801 gcagagtgct gggcttgttc aactcggata tatggcacta tgttctccaa cctgagtcag
     1861 gtaaccttaa aatgtcaggt gaagaaatag aaattaagat tgcggaaaga aatgaagcca
     1921 ggaaacagaa agacttcaca aaggccgatg agattcgtca gttcctcgca tcccacggca
     1981 tcattatcga ggacaaacca gacggcacaa gccggtggaa gcggtagccc gccggatctg
     2041 ctgtacggat tgcatgccgt tcgcgaggcg ctccgcgcga agactcgccc tcttcaacgg
     2101 gtgctcgttc ttcgcataga tcggcaattt gccgatcttg tccagctcgc aaaggcacaa
     2161 caagttccgg tccacatcga gcctcaaccg gcctttgatc gcttggtccc tcatgggagg
     2221 caccaaggtg tcatcgcatt cgtcgcggca aaatcctaca gcagcacgga ggacattctg
     2281 caacgggcaa aggagcgggg tgaaccgccc tttcttgtgc tcctcgatgg agtcgaagat
     2341 cctcataacc tcggcgcg
//
LOCUS       DATDNW010000241         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1329624, whole genome shotgun sequence.
ACCESSION   DATDNW010000241 DATDNW010000000
VERSION     DATDNW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329624"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VI359_05915"
     CDS             complement(<1..436)
                     /locus_tag="VI359_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6973821.1"
                     /translation="MLIELVLAGNSDLPLLAKLYDRIVAEGTSYPHERLLTHDEFQDY
                     WVRGKSTVVAHERPRKTEPELLGAFYLKPNWPGRARHVANAGFIVTPERRNHGLGWLL
                     GAVMLDYAKELGYRSVIFNLVFSENEVARHLWEKLGFRAIGVV"
     gene            complement(509..931)
                     /locus_tag="VI359_05920"
     CDS             complement(509..931)
                     /locus_tag="VI359_05920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY6973822.1"
                     /translation="MAGKILLEVVTPEKLLLSQQVDELIAPGVEGEFGVLPGHTYFLS
                     MLKIGELRYRVGDQTSYMSVLWGFAEVTPKKVTILAEIAEKAEDIDVERATQKVVEAE
                     ERLKVGGLPSDLKEAQISLEKARLRKRIAERASKTGHI"
     gene            complement(1046..2494)
                     /gene="atpD"
                     /locus_tag="VI359_05925"
     CDS             complement(1046..2494)
                     /gene="atpD"
                     /locus_tag="VI359_05925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY6973823.1"
                     /translation="MATATGKVIQVIGPAVDVEFPPGQLPNIYNALTVNQAENKAAGK
                     PAIRITLEVAQHLGENRVRSVAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLINVL
                     GEPVDEKGPIKTKKTYPIHRPAPKLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFG
                     GAGVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDFTKS
                     KAALVYGQMNEPPGARLRVGLTGLTVAEYFRDEENQDVLLFIDNIFRFTQAGSEVSAL
                     LGRMPSAVGYQPTLSTEMGALQERITSTKRGSITSVQAVYVPADDLTDPAPATAFAHL
                     DATTVLSRQQAELGIYPAVDPLDSTSRILDPQIIGEEHYKVARGVQSILQRYKDLQDI
                     IAILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEIL
                     DGKYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            complement(2587..>3252)
                     /gene="atpG"
                     /locus_tag="VI359_05930"
     CDS             complement(2587..>3252)
                     /gene="atpG"
                     /locus_tag="VI359_05930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY6973824.1"
                     /translation="KRPTKMVRVVVVTSDRGLCGGFNANIVRKALEFIRECEARGVKV
                     EIGLVGRKGRDFFKRRHWPTHEPYVDIFDRLSYEHGMEIAQRALEMYTQGAFDEAHII
                     YNEFKSALQQRVIVEQLFPIESLEENGQPREILGGSYLYEPDELELLDPLLMKHLQAQ
                     TYRILLESSAAEQAARMAAMDGATRNAGEVIKKVTLYYNKTRQAAITKELMDIVGGAE
                     ALK"
BASE COUNT          626 a          969 c          900 g          757 t
ORIGIN      
        1 gaaccacacc aattgcgcgg aagccgagct tctcccacag atgacgcgca acttcatttt
       61 cggaaaacac gagattgaag atcacgctgc gatagccaag ctccttggcg tagtcgagca
      121 tcaccgcgcc gagcagccaa cccaggccgt gattccgccg ttccggcgtc acaatgaacc
      181 ccgcattggc gacatgcctc gctctgccag gccagttggg tttgaggtag aacgccccga
      241 gcagttcagg ctctgtttta cgcggtctct catgtgctac caccgtgctc ttccccctca
      301 cccaatagtc ttggaattcg tcatgggtca gtagccgctc atgtggataa gatgtgccct
      361 cggcaacgat gcggtcatag agcttggcca gcagcggcaa atcggagttc ccggccaaca
      421 ccagctcaat cagcatcccg ttcttgagcc tagcttcaac agggaactgc atggctgata
      481 agagaattgt gcggattgtt tctggtacct agatgtgccc ggtcttgctg gcccgttcag
      541 caatcctctt gcggaggcgg gctttttcga ggctgatttg agcttcttta agatcggacg
      601 gcaggccgcc cactttgagg cgttcctcgg cctcgaccac tttctgcgtg gctcgctcga
      661 catcaatatc ctcggccttc tcggcgatct cggccaagat cgtcaccttt ttcggtgtga
      721 cctcggcaaa tccccagagg acggacatgt agctggtctg atcgccgaca cgatagcgca
      781 gctcaccgat cttgagcatg gaaagaaagt aggtgtggcc gggcaggacg ccaaactcac
      841 cctccacgcc tggcgcaatg agctcatcca cctgctgact cagcagcaac ttctccggcg
      901 tcacgacttc taacagaatc ttccccgcca tcttcgtcta actcctttgc tacaaagggc
      961 tagaaggcag gatgctctga cgtgcgcgca tcgaccgagc accttctgag cgtgcgcggt
     1021 ctgcgagcac aagagcgcct gacttctaga ccttgacccc catcttttcc gccttcgcca
     1081 ccgcttcctc gattggcccc accatgtaga aagcctgttc cggcaggtgg tcgtattttc
     1141 cgtcgaggat ctccttgaag ctacggaccg tgtccttgag ttttacgtac ttgcctggag
     1201 caccggtgaa ggcctcggcg acgtggaaag gctgtgagag aaaccgctga atctttcgag
     1261 cacgggccac gaccatcttg tcgtcttccg atagctcgtc cattccgaga atcgcgataa
     1321 tgtcctgcag gtctttgtac cgttgcagga tcgactgcac accgcgcgcc accttgtaat
     1381 gttcctcgcc gatgatttgc ggatcgagaa tacgcgaggt ggagtcgagc gggtcgacgg
     1441 ccggatagat gcccagctct gcttgttgcc gtgacagcac cgtggtcgcg tccaaatgcg
     1501 cgaaggcggt tgccggcgcc ggatcggtca agtcgtcggc cggcacatag acggcctgca
     1561 ccgaggtgat cgatccacgc ttggtcgagg tgatccgttc ttgcaaggca cccatttcag
     1621 ttgagagggt gggctgatag ccgactgcgg acggcatacg gccgagcaat gcggacactt
     1681 ccgaaccggc ttgggtaaac cggaagatgt tgtcgatgaa cagcaagacg tcctgattct
     1741 cttcgtcgcg gaaatattca gccaccgtga ggccggtgag accgacacgg agacgcgcac
     1801 cgggcggctc attcatctgg ccatacacga gcgccgcttt agatttcgta aaatcatcgg
     1861 gatcaatgac cttggactcc tgcatttcgt gccagagatc gttgccttcg cgggtccgtt
     1921 cgcctacgcc agcgaatacg gagaagcctc cgtggtggag agcgatgttg ttgatgagct
     1981 ccatgatgat gacggtcttg ccgactccgg ccccgccgaa gagtccgacc ttgccgcctt
     2041 tgctatacgg ctcaagaagg tcgacgactt tgatcccggt ctcgagcact tctgtcttgg
     2101 tgtcctggtc ttccagtttt ggagcgggcc ggtgaatagg ataggtcttc tttgtcttga
     2161 tcggaccctt ttcgtcgacc ggttcgccga gcacgttgat aaggcgtccg agcgtttcgc
     2221 gaccgactgg gacagagata ggtgccccgg tatcctgcac gtccatcccg cgtgtgagac
     2281 catcagtcgt ggacatggcc acactacgga cccggttttc accaaggtgt tgggcgacct
     2341 caagggtaat ccggatggct ggtttgcctg cagccttgtt ctccgcctga ttcaccgtga
     2401 gcgcattata gatgttgggc agctggccgg gaggaaactc cacgtctacc gccggtccga
     2461 tcacttgaat gactttgcct gttgcagtcg ccactggatg ctcctttatc tagagtgcag
     2521 agggtctggc actgagtgcc gagtcgaatg ctcgacactc agaacgtgca tctttgcagt
     2581 tccgttctat ttcaatgctt cggctccacc tacgatgtcc atgagttctt tcgtaatcgc
     2641 cgcctgtcgt gttttattgt agtagagcgt gactttcttg atcacctcac ccgcgttgcg
     2701 cgtggcacca tccatcgcag ccatccgagc ggcctgctct gccgcggacg attcaaggag
     2761 aatcctgtac gtctgcgcct gcaggtgctt cattaacaga gggtccagca gttccagttc
     2821 atcaggctcg tagaggtagc tcccccccaa aatctcgcgg ggttgtccat tctcctcaag
     2881 cgattcgatg gggaagagtt gctcgacgat cacccgctgc tggagcgctg acttgaattc
     2941 attgtagatg atgtgggctt cgtcgaacgc tccctgggta tacatctcaa gggctctctg
     3001 cgcgatctcc atcccgtgct cgtaagacaa tcggtcaaag atgtccacgt acggctcgtg
     3061 cgtcggccaa tgtcggcgct tgaagaagtc ccgccctttg cgccccacga ggccgatttc
     3121 cactttgacg ccgcgagcct cacactcgcg gatgaactcc agcgccttgc gaacgatatt
     3181 ggcattaaac ccgccgcaca atccccggtc gcttgtcacg acgacgactc gcaccatctt
     3241 ggtggggcgt tt
//
LOCUS       DATDNW010000242         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_303729, whole genome shotgun sequence.
ACCESSION   DATDNW010000242 DATDNW010000000
VERSION     DATDNW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303729"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VI359_05935"
     CDS             complement(<1..341)
                     /locus_tag="VI359_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973825.1"
                     /translation="MLHRMALRVLPSLSLAVTEESVRKQKRIVMLVPGLVAFGVYRLA
                     KHLLPLSEPVVLLLVSGAVAAVTSLMAYRVSRAESWPTIVRQDGNRLLAWLTGWIGFV
                     YGVQLSLLVLAL"
     gene            complement(350..823)
                     /locus_tag="VI359_05940"
     CDS             complement(350..823)
                     /locus_tag="VI359_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEY6973826.1"
                     /translation="MHTEGIEERAYNDGALQWFIRSECRGCGLKVGIDVPAGQTHGLV
                     DRVIWTDDALHRLDRLPPYVAPLFREEVERDVCARGERVVAYDTLFRLRTGERIVWEP
                     EAERRLDNVPAPVRAMAKIELERTAAERGETRVTVTLMEEVKAKYFGMAASKQGS"
     gene            complement(855..1568)
                     /locus_tag="VI359_05945"
     CDS             complement(855..1568)
                     /locus_tag="VI359_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEY6973827.1"
                     /translation="MDWLRNPGFFGTHATIGADLSQLMATLFTGLFVFGWVQAKKRQA
                     DTHHWLMLGGMVAMLGFFVSYYLFRNLGVLAFEGKEGFGGSELLYHNVFIPLLTFHII
                     LVVIGLVMAIYMIVLGFRAQAIAQGRRILSGRILQTTWGKIGMIFGVLCALVGVYLLY
                     LVAADRFRMGRLVVWVGLLALVAIVFAVEMAIQRIWPDGGQRHRTLGRFTMIIYCVLF
                     VTGTTTYTMLYILYPGKIG"
     gene            complement(1576..>2088)
                     /locus_tag="VI359_05950"
     CDS             complement(1576..>2088)
                     /locus_tag="VI359_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY6973828.1"
                     /translation="GPIRMLDLGGGAGTNAIAFCRVYQELTATVLDLPATLPLTERTV
                     KEAGLESRIALRSGDFNRDGLGGPYDFVLMSDILHYQNFATNASLVQKVYSHLAPGGR
                     LVIKDRFLDETGTGPAWTTAFAVHILVNTQEGSCYKTADAIHWMTQAGFHSVSELERT
                     AVVQGMRAAR"
BASE COUNT          482 a          668 c          531 g          407 t
ORIGIN      
        1 agtgccagca cgagcaacga cagttgcacg ccatagacaa atccgatcca accagtcagc
       61 catgccagca atcgattgcc atcctgccgg acaatcgtcg gccaggactc cgctcgacta
      121 actcgatagg ccatcaacga cgtcacggcg gcaactgcac cgctgaccag cagcagcacc
      181 accggctcgg acagcggcag cagatgtttg gccaaccgat acacaccaaa cgccaccagc
      241 cccggcacca acatcacgat ccgcttttgt ttgcgaaccg actcttccgt cacggccaga
      301 ctcaaactcg gaagcactcg taaggccatt cgatggagca tcgtgatttc tatgacccct
      361 gttttgaggc agccatgccg aaatacttgg ctttcacttc ttccatcaga gtcacggtga
      421 cgcgagtctc tccccgttct gcggccgtgc gctcgagttc tattttcgcc atggcacgaa
      481 ccggggccgg tacattgtcc aaacgacgtt ccgcttcagg ctcccaaacg atacgctctc
      541 ccgtgcgcag ccgaaacaac gtgtcatagg ccaccactcg ttcgccgcgc gcgcacacgt
      601 cccgttcgac ttcttcgcgg aacaacgggg ccacgtaagg aggcagacga tcgagccgat
      661 ggagcgcgtc atccgtccat atcacccgat cgacaagccc atgtgtttga ccggccggca
      721 cgtcgatccc cacttttagt ccgcacccgc gacattcgct ccggatgaac cactgaagcg
      781 ccccatcgtt ataggctcgt tcttcaatcc cttccgtatg catccagcgg ccgcatccac
      841 aggtcaacat gcgattatcc aatcttgcct gggtagagga tgtacagcat cgtgtaggtg
      901 gtcgttccgg tgacaaacag aacgcagtag atgatcatcg taaagcgccc gagtgtccga
      961 tggcgttgtc cgccatccgg ccagatacgc tggattgcca tttccacggc gaacacaatc
     1021 gcgacaagtg caagaagtcc gacccacacc acgagccgtc ccatccgaaa acgatctgcg
     1081 gccaccagat acagcaagta cactcccacc agggcacaga ggacaccgaa gatcatgccg
     1141 atcttccccc aggtcgtctg aaggatgcgc ccgctcagga tccgtcgccc ttgtgcgatt
     1201 gcctgggctc ggaatcccag gacaatcatg tagatcgcca tcaccagtcc aatgaccaca
     1261 agaatgatgt gaaaggtgag gagagggatg aacacattgt gatagaggag ttcagatcca
     1321 ccgaaccctt ctttcccttc aaaggccagc acgcctaggt ttcggaagag atagtaactg
     1381 acgaagaacc ccagcatggc caccatgcct cccaacatca gccagtggtg cgtgtctgcc
     1441 tggcgcttct tcgcttgaac ccaaccgaac acgaacagac cggtgaagag cgtagccatg
     1501 agttggctga ggtcggcgcc gatcgtggca tgagttccaa agaatccggg atttcgtagc
     1561 cagtccatgg cgcaattacc tcgcggccct catcccttgc acgaccgctg ttcgctccaa
     1621 ttcacttact gagtggaatc cggcttgagt catccagtga atggcatccg ctgttttgta
     1681 acaactaccc tcctgcgtgt tcaccagaat gtgcacggca aacgctgtgg tccaagccgg
     1741 accggttccc gtttcgtcca ggaatcgatc cttgatcacc aggcgaccac cgggcgcgag
     1801 gtgcgaatag actttctgaa ccaacgacgc gttcgtggca aaattttgat aatgcagaat
     1861 gtcggacatg aggacgaagt cgtatggtcc acccaggcca tcccgattaa agtcacctga
     1921 gcgcagcgcg atcctggact ccaacccggc ttccttcact gttcgttccg taagtggcag
     1981 agtggcaggc aaatcgagca ccgtggcggt cagttcctgg tacacgcggc agaacgcaat
     2041 ggcgttcgtc ccggccccgc ctcccaagtc cagcatgcgg atcggccc
//
LOCUS       DATDNW010000243         5735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_30125,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000243 DATDNW010000000
VERSION     DATDNW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30125"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1813
                     /locus_tag="VI359_05955"
     CDS             98..1813
                     /locus_tag="VI359_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY6973829.1"
                     /translation="MSTVSKQSDQQLDRAALAALYEETFRNLEEGTITEGRVVALTKD
                     KVVVDIGYKSEGMIPSDQFSAEELQQIKVGDRLQVYIEECEDAEGNLVLSKEKADKMK
                     IWEELEKLHKEEKSIEGKIISRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGRVV
                     PLKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLSTLKEGQLIHGTVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSEMFTVGDRVEVTVLKYDRETGRISLGLKQKTADPWT
                     NVANKYPIGTRIKGRVVSLTDYGAFVEIEPGVEGLVHVSEMSWTHEVRHPSRVVSIGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWNMVEEKYPVGTRIEGKVKSLTDFGAFIGLD
                     EGIDGLIHISDMSWTKHVKHPSELFKKGQKVEAVVLRLDKEKERLSLGFKQLTRDPWD
                     DEIPSRYRVGDAVNGKVSKVADFGIFVELGGGVEGLIHVSEIGLDHHVKLEEKFKLQD
                     DVAAKIIKVDREERKIALSLRDLQLDSERQQVDEYHATQGALDQSLGRAAKQSRKRGQ
                     AEDEK"
     gene            1903..2799
                     /gene="sppA"
                     /locus_tag="VI359_05960"
     CDS             1903..2799
                     /gene="sppA"
                     /locus_tag="VI359_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY6973830.1"
                     /translation="MSEEHSGKKTKWSAWRIVRWVLGGGLLLLVLINLFFPDLDLSTE
                     DRIALVRIEGVILDSQTTVGELKRFGENPSIKAIVLRIDSPGGGVVPSQEIYDAVQRV
                     RNKSNKAVIASMGTVAASGGYYIAAATDRILANPGTLTGSIGVIMEMANIEGLLKKIG
                     VEGVVIKSGRYKDVGSPLRKMSDEEQALLQSVMDDVHKQFIEAVAAGRSLEVADVKAL
                     ADGRIFTGRQAKDAKLVDELGNLEDAIQLAADISGIEGEPKVVEPRRRFSIRELLESR
                     LPTLFPKLDFHTGVSLKYLMAY"
     gene            2862..3140
                     /locus_tag="VI359_05965"
     CDS             2862..3140
                     /locus_tag="VI359_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY6973831.1"
                     /translation="MTKAQIIERVSEQVATLTKRQAEVVVNTIFDCVRDSLKNGDKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKKVPFFKAGKELKELLNS"
     gene            3158..4159
                     /locus_tag="VI359_05970"
     CDS             3158..4159
                     /locus_tag="VI359_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEY6973832.1"
                     /translation="MNSSEPNESVATDVRWTDGAVKRMERAPVFLRGMVRRLAEKKAR
                     ELGYTEITEEILDQFKSQMMGRMGGESGMSAAAEQLEQGNLPWTAAAKERLATVPEFM
                     RGMIKQIAEEIARERGHLEVNVDLFEKVEALGDLRETDGPPLEWTEGALARLQEKLKQ
                     SPPIAVEFVTDMLKRDTEDLAREKGLGYIDEATLLALWDAPQERVAWTDEAWKRLQTS
                     PDFVRSGIRKAAERRARKLGLKEIDSDHLTTFRNQAMMKAVKRIRSFGYNELTFDAFD
                     TAMAKTKRLQGNEQAEKRLQEIRGHFADPNAKKPEGGTLGAELMDRFRRYLKGEGTL"
     gene            complement(4324..4644)
                     /locus_tag="VI359_05975"
     CDS             complement(4324..4644)
                     /locus_tag="VI359_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973833.1"
                     /translation="MGNIIKMPLTMYGVAEVLHWCHDRNKGRVPGVDTTGFEKMKALL
                     AEKPQGADYFTLDQFWKKKVMLELTEEEIATIDRCLYDIPNFDNEPLPQIRHKFWPQQ
                     SATH"
     gene            4885..>5735
                     /locus_tag="VI359_05980"
     CDS             4885..>5735
                     /locus_tag="VI359_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY6973834.1"
                     /translation="MTARVQSRDSHITSMLIAVSILSHLYTGGCSKQDPYIPPDLFYY
                     FASYKVGKNPTTVNPLDLNQDGITDLVTTNMGSNTLSILLGNGDGTFRDQMQLHVCRE
                     PRSMAVGLFNRDQYPDVVLACSGSDEIAVLFGRADGKLDEGPQYPVHRTPVAVTSGDL
                     NGDHVPDLVVALRNDKIKVFLGTGTGEFQHGAQYEYGDTPTSVALADLNGDGKLDLAV
                     TNGGPMSNAISIWIGNGDGTFRGPTDYRSGKRPLGISFADFNDDRVQDMLVINGERDS
                     FTTFLGKG"
BASE COUNT         1427 a         1388 c         1709 g         1211 t
ORIGIN      
        1 ccgatccgtc acgacacagc cggaacccgc agcgctgagt gagttccggt tttattacta
       61 tcagcacccg acttgttcgg aagagtagga agctcccatg agtacggttt cgaaacagag
      121 tgaccaacag ttggatcgtg ccgcattggc ggccctctat gaggaaacat tccgtaacct
      181 cgaggaaggc accattacgg aaggccgcgt tgtggccttg accaaagaca aggtggttgt
      241 ggacatcgga tataaatccg aagggatgat cccgagcgat caattttccg ccgaagagct
      301 ccagcagatt aaggtcggcg atcgtctcca agtgtatatc gaggaatgcg aagacgcgga
      361 gggcaatctc gttctctcca aagaaaaagc cgataagatg aagatttggg aggaactgga
      421 gaagctgcat aaggaagaaa agagtattga aggcaagatt atctcccgaa tcaaaggcgg
      481 catgatggtg gacatcggcg tcaaagcctt tctcccgggt tcgcagatcg atttacatcc
      541 ggtacgcgat ctggatggtc tcgtcggtcg cgtcgtgccg ctcaagatca tcaagatcaa
      601 tcatcgccgc ggcaatgtgg tggtgtcgcg ccgggttctc ttggaggaaa ctcgcgacaa
      661 gaagcgacag acaaccttgt cgaccttaaa agaagggcag ttgattcacg ggactgtcaa
      721 gaacatcacg gactacgggg cctttatcga tctcggcggc atcgacggcc tgcttcacat
      781 caccgacatg tcgtggggac gagtcggtca tccctccgaa atgtttaccg tcggtgaccg
      841 ggttgaggtg acggttctca agtatgatcg agagacaggc aggatttcgc tgggcctcaa
      901 gcagaagacc gcggatccat ggaccaacgt ggcgaacaaa tatccgatcg gcactcgtat
      961 caaaggacgg gtggtcagcc tgacggatta tggggcgttc gtggaaatcg aaccgggcgt
     1021 ggaaggcttg gtgcatgtgt ccgagatgtc ctggacacac gaagtccgcc atccgtcccg
     1081 tgtggtatcg attggtgacc aggtcgaggc agcggtgttg aacgtggacc ctgccagccg
     1141 caagatctct ctgggcatga agcaaaccgc ccccaatccc tggaatatgg tggaagagaa
     1201 atatcccgtt ggaaccagga ttgaaggcaa agtgaagagc ctcaccgatt tcggcgcatt
     1261 catcggtttg gatgaaggaa tcgacggctt gatccatatt tccgatatgt cgtggaccaa
     1321 gcacgtcaag catccgtccg agctctttaa gaaggggcaa aaagtcgagg ccgtcgtgct
     1381 acgccttgat aaagagaagg aacgactgtc tctagggttc aagcagctga cgcgcgaccc
     1441 ctgggatgat gaaattccga gccgctaccg agtcggtgat gcggtcaatg gtaaggtcag
     1501 caaggtagcg gacttcggaa ttttcgttga attggggggt ggggttgaag ggctgattca
     1561 tgtcagtgaa attggccttg accatcacgt caagctcgaa gagaaattta aattgcagga
     1621 cgacgtggcg gccaaaatca tcaaagtgga tcgcgaggaa cggaagatcg cactcagtct
     1681 gcgtgaccta caacttgatt cggagcgcca gcaggtcgac gagtaccacg ctacgcaagg
     1741 cgcgttggat cagagcttgg ggcgtgcggc caaacagagc cgcaaacgcg ggcaggctga
     1801 ggacgagaaa tagtcaaggc cacgcgactt cgctcgaaca ttcgtgagcg aaggttgtgg
     1861 tctgacggct caacgtatcg gactcatgct caagagtgac acatgagtga agaacattcg
     1921 ggtaaaaaga ccaaatggag cgcttggcgt atcgtgcgtt gggtgctcgg cggcgggctt
     1981 cttctgctcg tgctcatcaa cctcttcttc ccggatttgg atttatcgac ggaggaccgg
     2041 atcgcactcg tgcgcatcga aggggtcatc ctcgattccc aaacgaccgt cggagaattg
     2101 aaacggttcg gagagaatcc ttcgatcaaa gcgatcgtct tgcgcatcga cagtcccgga
     2161 ggcggcgtcg tgccgtcaca ggaaatctac gatgccgtgc agcgcgtccg aaacaagagt
     2221 aataaagccg tcatcgcatc gatgggtacg gtcgcggcgt caggcggtta ttatatcgct
     2281 gccgccaccg atcgaattct ggccaatccc gggacattga ccggaagtat cggtgtcatc
     2341 atggagatgg ccaatatcga aggcctactg aagaaaatag gagttgaagg agtagtgata
     2401 aagagcgggc gctataaaga tgtcggctcg ccgctgagga aaatgagcga cgaggagcag
     2461 gccctactgc aatccgttat ggacgatgtc cacaaacagt ttatcgaggc agtcgccgcc
     2521 ggccgatccc tcgaagtggc cgatgtaaag gcgctcgccg acgggagaat tttcaccggc
     2581 agacaggcga aagatgcaaa acttgttgat gagttgggga atctcgagga cgccattcag
     2641 cttgcggcag atatctctgg aatcgaaggc gaaccgaaag tcgtggagcc tcgccgacgg
     2701 ttttctatca gggagctgct ggagtcgcgg ttgcccacgt tgttccccaa gctggatttt
     2761 cacacgggtg tcagcctgaa gtatctcatg gcgtactaga tgccggtctt ggggcagtga
     2821 ataatcatga tggtcgcgtt gagaagggga gggaatccga tatgacgaaa gcgcagatta
     2881 ttgagcgagt ctcggaacag gtcgccacgt tgacgaaacg tcaggcggag gtcgtggtca
     2941 atacgatttt cgattgcgtg cgagattcgt tgaagaacgg cgataagacg gagatccgag
     3001 gctttggcag ctttcgactg cgggcgcgtc ggatgaaaga aggaaggaat cctaagacag
     3061 gggccacagt ggcggtcccg gccaagaaag tcccgttctt caaagccggc aaggaattga
     3121 aagagttgct gaattcataa cgaaggaagt tagaacaatg aattcctccg agccgaatga
     3181 atcagtcgca accgacgtac ggtggactga cggagcagta aaacggatgg agcgtgctcc
     3241 agtgtttttg cgagggatgg tgcgtcgtct tgctgaaaag aaagcgcgcg aactgggtta
     3301 taccgaaatc accgaagaga tccttgatca gttcaagagt caaatgatgg gtcggatggg
     3361 tggtgaatct ggcatgtcgg cggcagccga acaattggag caggggaatc ttccgtggac
     3421 ggcggcagca aaagagcggc tcgcaacggt tcccgagttt atgcgcggta tgatcaaaca
     3481 gattgccgaa gagatcgcac gagaacgcgg gcatctcgaa gtaaacgttg atctgttcga
     3541 aaaggttgaa gcgttgggcg atctgcgtga aactgacggt ccgccgctcg aatggacgga
     3601 gggagcgttg gctcgacttc aagagaaact gaaacagtct cctccgatcg ccgttgagtt
     3661 tgtgacggat atgctcaaaa gagacaccga ggatcttgcc cgagaaaagg gtctgggtta
     3721 tatcgatgaa gcaacgttgc tggcgttatg ggatgcaccg caagaacggg tggcctggac
     3781 tgatgaggcg tggaaacggt tgcaaacatc gcccgacttc gtccggagcg gaattcgtaa
     3841 agccgcggaa cggcgagccc gcaagctcgg tctcaaagag attgactcgg accatctcac
     3901 cacgttccgg aatcaagcca tgatgaaagc cgtcaagcgc atccgttcct ttggatacaa
     3961 tgagctgacg tttgacgcgt ttgacacggc gatggcgaaa accaagcgcc ttcaaggcaa
     4021 cgagcaggcg gagaagcgtc tccaagaaat tcgtggacat ttcgctgatc cgaatgcgaa
     4081 aaagccggaa ggtggcaccc tcggtgctga actcatggac cggtttcgtc ggtacctcaa
     4141 gggcgagggg acgctctaga acctttttcg tcgattctgg tacgctgacc cgcaggattc
     4201 gagccttcca accctgacaa tagcacacgg ttcgtgtgca gtgagtcctt gcaggatgag
     4261 gtcgggtaat cgaatgtctg aaagataagg aagggattga aacggtccaa ccccttcctc
     4321 tgattaatgc gtagccgact gttgtggcca gaacttgtga cggatctgtg gaagtggctc
     4381 gttgtcgaag ttggggatat cgtagaggca ccggtcgatc gtagcgattt cttcctcggt
     4441 caactccagc atgaccttct tcttccaaaa ctggtcgagt gtaaagtaat ccgctccctg
     4501 cggcttctca gctaagagcg ccttcatttt ttcaaagccc gtcgtatcga ccccaggcac
     4561 gcggcctttg tttcggtcgt ggcaccaatg caggacttca gccactccgt acatggtcaa
     4621 cggcatcttt ataatgttcc ccatgtcaat cctccttcaa aagagcgcta ttctatccca
     4681 gacagtccac gattatcaaa gcctcggaag gtggccaaag accccaaaga ggttgttcga
     4741 aaatcgggtc aggcggccag ttgattgcca gggccgggag tggtctgtat actgcccaga
     4801 ctttcctgac ggtggccaga aggaaatgcc gaactcgtac gccaggaccc gtcaagaacc
     4861 cagtttttga acgaggagtt gagtgtgacg gcgagagtgc agagccgcga tagtcatatt
     4921 accagcatgc tgatagcggt ctcgattctc tcccacctct acacgggagg atgttcgaag
     4981 caagacccat atattcctcc agatctgttt tactacttcg caagttacaa agttgggaaa
     5041 aatccgacaa cggtcaatcc acttgatctc aatcaagacg gcatcaccga tcttgtcacc
     5101 acaaacatgg ggagcaatac tctctcgatc ttgttaggga atggagacgg cacatttcgt
     5161 gatcagatgc aacttcatgt gtgccgggaa cctcgttcga tggcggtggg cttattcaat
     5221 cgggatcaat acccggacgt tgtcctcgcc tgttccggga gcgatgaaat cgccgtcctt
     5281 ttcgggcgcg ccgatgggaa actggatgaa ggtcctcagt acccggtgca tcgcacgccg
     5341 gttgccgtta caagcggcga tctcaacggt gatcacgttc ctgatctcgt cgtggcactg
     5401 cggaacgaca agatcaaggt gtttttggga acaggcactg gcgaatttca acacggagcg
     5461 caatacgagt atggtgatac gccgacatcg gtggcgttag ccgatttgaa cggcgatggc
     5521 aagttggacc tcgccgtcac caacggtggg ccgatgtcaa atgcgatttc gatctggatc
     5581 gggaatggag acggaacatt tcgaggcccg acggattatc ggagcgggaa acgtccactg
     5641 ggtataagct tcgcggactt caacgacgat cgagtccaag acatgcttgt catcaacggt
     5701 gaacgagaca gttttacgac tttcctgggt aaggg
//
LOCUS       DATDNW010000244         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1062519, whole genome shotgun sequence.
ACCESSION   DATDNW010000244 DATDNW010000000
VERSION     DATDNW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062519"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..612)
                     /locus_tag="VI359_05985"
     CDS             complement(298..612)
                     /locus_tag="VI359_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY6973835.1"
                     /translation="MKRTATIAVISLFTAGLCVAPFALAAPDQQNKMKACNEQANAKG
                     LGEGKGDERKAFMKECLSAKPAKSGKTAQQEKMKACNKEAGEKQLKGEERKKFMSTCL
                     SG"
     gene            complement(703..1440)
                     /gene="truA"
                     /locus_tag="VI359_05990"
     CDS             complement(703..1440)
                     /gene="truA"
                     /locus_tag="VI359_05990"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY6973836.1"
                     /translation="MPTFKLTLEYDGTGYAGWQRQPNQPTIQEAVERAILQVSQSSVS
                     VIAAGRTDAGVHALGQVASFRTDRTWTPADWTRALNAVLPEDISVRTTELMPDAFHAQ
                     HAARAKLYEYRILNRPERPTIERRYVWHIYRSLDDAAMQQATSTLIGSHDFSSFEGSL
                     TDNDDPRCNLHRLTLTRRGDHLSFEAYGDRFLKHMVRAIVGTLVEVGTGKRSPRSLAD
                     VLHARDRSAAGQTAPPHGLFLIHVAYE"
     gene            complement(1469..>2490)
                     /locus_tag="VI359_05995"
     CDS             complement(1469..>2490)
                     /locus_tag="VI359_05995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY6973837.1"
                     /translation="RRTRVKKHFIPKPNGIIAEIPNATLSKSAQTKLAGGSIAKPFVI
                     TQISSHSVAVSLPNGSFQRYHVLSLTNPPRLVVDVVPSNQPSVTQPIEAIDPSLPVTP
                     PSQPVPPPTKSFRTIVIDPGHGGRDPGARGLRGTEEKDITLKVGLKLRDLLSRQPGVR
                     VMMTRDRDVFVELEDRAKYANNLEADLFVSIHVNSHPQRTVKGIEIYHFGEAKDQRAL
                     EVAARENGTPLSSTGVGWEYLVADLLTTKKIEESLELAWTTKEAMVAHMNGHYATVDH
                     GVKTAPFYVLRFTSMPSILAEIAYVSNPSEEGLLRTDTFVSRVAESLNDGVKAFLSSA
                     KQPAR"
BASE COUNT          490 a          686 c          751 g          563 t
ORIGIN      
        1 agaatcatca agacacaagg tgagcggatg cccgatgcga cgatggatca caccaatatg
       61 ccagacccta tcagtgatcg tttcaccgct ctcgaacagg aattgatcga actccgcaag
      121 aaactcgaaa accgcgacga aagtccctcc tccgctcggt gaaccacatt ctttctcaaa
      181 ccggcggcca gttcttcgaa gacaggaagc ggtcgcatcg ctgactggca ggtaacttcc
      241 tgacaatgac cggtgagagg gcgacgcggc ccggcctgac cgcgtcggtg aagaggacta
      301 gccggacagg caggtgctca tgaacttctt ccgctcttcg cccttcagct gcttctcgcc
      361 cgcctccttg ttacacgcct tcatcttctc ctgctgagcc gtcttcccgc tcttcgccgg
      421 tttggcggag agacattcct tcataaacgc cttccgttca tcgcccttcc cttcgcccaa
      481 gcctttggca ttcgcctgct cgttgcaggc cttcatttta ttttgttgat cgggggcggc
      541 aagcgcaaac ggcgcaacgc acaagccggc tgtaaaaaga gagatcactg cgattgtcgc
      601 tgtcctcttc atgtgtgtct ccttttgtga aggcgaagga gtcgatgcgg cagcatgata
      661 gcaaaatcca tcagggttgt cgctgggccg atcgcccagg actcattcat aagcgacgtg
      721 gatgagaaac agtccgtggg gaggagcggt ttgtcctgcc gccgatcggt cgcgggcgtg
      781 caacacgtcc gccaggctcc ggggcgatcg tttcccagtc cccacctcaa ccagagtacc
      841 gacgatggcg cggaccatgt gtttgaggaa ccgatcgccg taggcttcga aggaaagatg
      901 atcgccgcgg cgagtcaagg tcagccgatg aagattgcat cgcggatcat cgttgtccgt
      961 gaggctgcct tcgaacgagg agaagtcgtg cgaaccgatc aaggtagacg tagcctgttg
     1021 catggccgca tcgtcgaggg atcgataaat atgccataca taccggcgct cgatcgtggg
     1081 ccgttccggc cggttgagaa tacggtactc atagagcttg gcacgcgcgg catgctgggc
     1141 atgaaacgcg tctggcatga gttcagtcgt acgcacagag atgtcctcag gtagcacggc
     1201 attcaaggcg cgtgtccagt ctgccggtgt ccatgttcgg tccgtccgaa aactcgccac
     1261 ttggcccagc gcatgcaccc ccgcatcggt acgaccggcc gcgatgacgg acacgctgga
     1321 ttggcttact tgcaggatcg cgcgctcaac cgcctcctga atcgtgggct ggttgggctg
     1381 ccgttgccag cccgcatagc cggtgccgtc gtactccaac gttaatttga aggtcggcat
     1441 gggaaatgcg cgtcaaccgg aatgcgaatc atcgagccgg ttgtttggct gagctgagga
     1501 aagctttcac gccgtcgttg agtgattcgg cgacgcggct cacgaaggtg tcggtgcgga
     1561 ggaggccttc ttcgctcggg tttgacacgt aggcgatttc cgccaagata ctgggcatgc
     1621 tggtgaagcg aaggacatag aacggggcgg tcttcactcc atgatcaacc gtggcatagt
     1681 gtccgttcat atgggctacc atggcttcct tggttgtcca ggccagttcc agcgattcct
     1741 cgattttctt cgtcgtcagg aggtcggcga cgagatattc ccaccctacg ccggtgctgc
     1801 tgagcggggt gccgttttca cgagctgcga cttcaagagc acgctgatcc ttggcttctc
     1861 cgaagtgata aatctcgatt cctttgaccg tccgctgcgg atgcgagttc acatggattg
     1921 agacaaacag gtcggcttcg aggttgttgg catacttggc tcggtcctcg agttcgacaa
     1981 agacatcgcg gtccctcgtc atcatgacac gcacgcccgg ttgtctgctt aggaggtcac
     2041 gaagtttcag ccccaccttg agcgtgatgt ccttttcctc tgtcccccgc agccctcgag
     2101 ctcctggatc tctgccgccg tggccgggat cgatcacaat cgttcggaat gatttcgtgg
     2161 gaggtggtac cggctgggag ggtggggtga cggggagcga cggatctatc gcttctatcg
     2221 gttgcgtcac gctcggctga ttggaaggca cgacatcgac gacaaggcga ggagggtttg
     2281 tcaggctgag cacatgatag cgctgaaacg atccgtttgg gagggagacg gcgacggaat
     2341 gcgaagaaat ctgggtgatc acaaaaggtt tggcgattga accgcctgca agcttggttt
     2401 gggccgattt actgagggtg gcgttgggaa tttcggcgat gattccgttg ggtttaggaa
     2461 tgaagtgctt cttgacgcgt gttcttcggt
//
LOCUS       DATDNW010000245         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_850787, whole genome shotgun sequence.
ACCESSION   DATDNW010000245 DATDNW010000000
VERSION     DATDNW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850787"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..931
                     /locus_tag="VI359_06000"
     CDS             530..931
                     /locus_tag="VI359_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973838.1"
                     /translation="MPGTILVVDDDHDVRDVVRLVLSREGYGVLTAPDGHSAMELMTS
                     PENAEKVCTVLCDLEMPNGSGTELIEYFRLHYPTIPIIVLSGSPDAKFLDGILQQGVC
                     DWIRKPATREILLEKVRTAANLFTLRKQQGT"
     gene            1092..1880
                     /locus_tag="VI359_06005"
     CDS             1092..1880
                     /locus_tag="VI359_06005"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEY6973839.1"
                     /translation="MNIRVLGCHGSDGLLTRERGTPECNTCGFLLDGTVLLDAGTVAS
                     KLLFEEQKQIRHVLLSHLHFDHIKGLPTFADNLSEDAASPVTIAAIPEVIAGLRRHIF
                     NTTVYPDFFTIPTPNAPTFVAQTIEAGKPYCVDGFEITPILVNHTVPAVGFIIRDQSA
                     ALLYSGDTYLTDELWSLAHHIPNLKAAFIECSYPDTKKDLAQISKHLTPSLLAKEFRK
                     IGRPDIAVYAYHLKPAFTDQIVRELKDMGIRYLSVLEEGQSISI"
     gene            2275..>2528
                     /locus_tag="VI359_06010"
     CDS             2275..>2528
                     /locus_tag="VI359_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973840.1"
                     /translation="MGTLMKSEHEVFAPYPTRRNGDSPTPHNATGVIFFNSTGQLLFM
                     NDKVPSFFQRLQPGSTRECGTWLIPAEIHTVVHELTTRLQA"
BASE COUNT          591 a          744 c          665 g          528 t
ORIGIN      
        1 aaccatccgt ggagttatcg agtgactgcg acccagttct tcgtaatgcg tggcccctcg
       61 acctgctcat aacagtgagg cctggaatcg agaatcgaac gacgcatggg tgtatggacc
      121 gtgatggatc cgtccaaggt ccgcatatac aacaagccgg acatatcggc agctctggca
      181 cgggcgggca atccgaatat ctgggtctct tgctgcaata gaatcttcaa tcggatgagc
      241 cggcgcttcc gaagagccaa gtatcatggc accctttcct gagttcctgc tcaatcacga
      301 cctgccattt ctcctgcccg ccatatccac aacctcggga gtatcccctc agatagctcc
      361 cccctcattt gggggcattc cgtctgagac aaaggtctac tacagtgcct cccgcatcgg
      421 ctcaccatca ggagagcaga acgctccctc acatggtgac gaaacagaac gcgcaagagg
      481 acacgtgttc gatcgagcac gtgtgacccc gtcggaggga gtctgtccta tgcccggaac
      541 aatcctggtg gtggatgacg atcatgacgt tcgggatgtc gtccgtttgg tgctcagccg
      601 ggaaggatat ggtgtgctca cggcaccgga cggtcatagt gccatggagc tcatgacctc
      661 acccgagaat gccgagaaag tctgcaccgt tttgtgcgac cttgaaatgc cgaatggaag
      721 cgggacagag ctcatcgagt attttcgtct gcactacccg accattccga tcatcgtgct
      781 cagcggatcc ccggatgcaa agtttctcga tggcattctg cagcagggag tctgtgactg
      841 gatccgcaaa cctgccacgc gcgagatttt gcttgagaaa gtccgaaccg cggccaatct
      901 ctttacactt cgcaaacaac aaggcacgta accttaaatg aacgtggaca acggggtatg
      961 gtagtgaata cagtactgag ccaagccggt cacagcactt cgacagacag gatatgcaga
     1021 gaacatccgc ttagagagag ttgacaggtt cgttgagtgg acaattaagc gcggcgtcgg
     1081 gagagaaggc agtgaacata cgcgtcctag gctgtcacgg atctgatggt ctgctgacca
     1141 gggagagggg gaccccggag tgcaacacct gcgggttctt actagacggc actgtgctcc
     1201 tcgacgccgg cacggtcgcc agcaagctgc tctttgaaga gcaaaagcag attcgtcatg
     1261 tgctactctc acacttacat tttgatcaca tcaagggctt acccaccttc gccgacaacc
     1321 tcagcgagga cgccgcctcg cccgtgacga tcgccgccat tcccgaggtc attgcgggac
     1381 tacgaaggca catcttcaac acgacggtct atcccgattt cttcacgatt ccaaccccga
     1441 acgctcccac ctttgtggcg cagacaatcg aggcggggaa gccctactgc gtggacggtt
     1501 tcgagatcac gcccatactc gtgaaccata cggttccggc ggtgggcttc attattcgtg
     1561 accagagcgc cgcactcctg tatagcggcg atacctacct gaccgacgag ttgtggtcgc
     1621 ttgctcatca catcccgaat ctcaaagcgg cattcatcga atgctcctac cccgatacaa
     1681 agaaggatct ggcgcaaatc agcaaacacc ttacgccctc attgctcgcc aaggaattcc
     1741 gtaagatcgg tcgccccgat atagcggtct atgcgtatca tctgaagccg gccttcactg
     1801 atcagatcgt ccgcgaactc aaggacatgg gcatccgcta tcttagcgtc ctcgaagaag
     1861 ggcagagcat ctcgatttag cacctgtgcg atccctgagc ccagagtctt cttcacgcca
     1921 ctcgcctctt gccccatctt cctcgccacc ccaccaggtt gaatcgaatt cgattcgctc
     1981 tgaatcgtat tcgaccaatc attgaggcca agaccgtcca ggctccatgg gcaccagccg
     2041 aaatcggcaa cgtacaatca ctcgattaga catggtgttg cgaggatccg ggttggccag
     2101 agacccgtcg gatctggtag gcctctctat tgcatgcaga tcgaatcgag gcagggcttc
     2161 caaagggagt tctgctctgt gacaacatcc tcggtagggc cgaccctctc gcgaaaacaa
     2221 gcggatcgag ggagtccgta ccggcggttt ttgggctgcc gaagggagta cgagatggga
     2281 accttgatga agtctgaaca cgaggtgttc gcaccctatc cgactcgaag aaatggagac
     2341 agtccgaccc ctcacaacgc gaccggcgta atcttcttca acagcacagg acaactcctc
     2401 ttcatgaatg acaaggtgcc gtcatttttt cagcggctac agccaggctc gaccagagag
     2461 tgcggtacat ggctcatccc cgcggaaatc cacacagtcg tgcacgagct gaccactcgc
     2521 ctacaggc
//
LOCUS       DATDNW010000246         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1192707, whole genome shotgun sequence.
ACCESSION   DATDNW010000246 DATDNW010000000
VERSION     DATDNW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192707"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /gene="uvrC"
                     /locus_tag="VI359_06015"
     CDS             <1..1731
                     /gene="uvrC"
                     /locus_tag="VI359_06015"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY6973841.1"
                     /translation="IGKAASLANRVRSYFLKGGDHSPKTTLLVSQIADLETIVTRSEL
                     EALILESNLIKRHRPKLNVVLRDDKQYPYLRLPIKEDFPRLSIVRRVQKDGALYYGPY
                     TPAGALRETLKVIKRVFPLATCTIEIDGTAERACIEFEIKRCMAPCTGNQSKEDYQKI
                     VTQVRQFLEGRDRELLDDLRAQMAAAAEREEFEEAARLRDRLFKIERTLEKQRVTQTT
                     AIDQDVIGVARQGTAVDLQLLFVRGGLLIGRKDFFWPQATEAGDEELVRSVIEQFYNK
                     DGQPPKELLLPVELGDSTLIEEWLSEKRGDKVRLVAPERGVKHQLLLLAEENATASVA
                     DHLRDELLDRQAGEELQRLLRLDRPPVRIEGFDISNTMGNQSVASMVVWERGQMKKSD
                     YRRFKIQTVTGANDFASMQEVVLRRYRDSEDLARPDLVLIDGGLGQLAAAREGLKQAG
                     EPAMAVIGLAKARGEKDERIFLPGRKNPILLRANSPATHLVQRIRDEAHRFALSFHRR
                     LRSKALLASQLDHIIGIGEIRRTSLLRRFKTLEGLAAASDEDLRATGLNSQTIQDLRT
                     QLGTKTTTRS"
     gene            1918..>3834
                     /locus_tag="VI359_06020"
     CDS             1918..>3834
                     /locus_tag="VI359_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6973842.1"
                     /translation="MDAPIDTSHQTSDDVISLRQRIAELEQTIAQQRLADAALRERQV
                     HFQTLYEDTPAMAFVLSMDGTILSLNRYGAEQLGYTLEELVGQSVLQIFDPADHHAVL
                     RQLTVCGHTPNNTLGWELQKVRKDGSRLWVRETARTIRDAAGSPTILVVCEDITTRKT
                     AEEELRVTAQKLQALIHACPLAVLSLDNEGETVTLWNPAAEAMFGWHADETLGRPTPF
                     LPPSQHKESGRMWDELITSGAVSGVEFRRVRKDGTPIDVSLWATVLKDERGIVTDTLG
                     FLADITARKQVEASLRESEQAIRTLQEAISRPTLTFDQRIQAMLELGCRRFKLPTGLM
                     TRVQGKDLVITHVSSQDANITAAMVLPLDQTYCHATLMAEGPVCFEQASASQWRDHPG
                     HATLGIECYIGTKLVGLNSIHGTICFVAPTPYAARFTDADKDFLLLMAQWIGHEQDRQ
                     ESERALKRHESLLRAVIDTATDAIFMKDTKGRYQLINAAGARTIGKPVEEIVGKTDLE
                     LFPPETAKQLMENDQRVLLGGSQHHFEAVIPFQGEARTFSSIKTPHRDPQGNVIGLVG
                     VSRDITDQKLLEEQRSKSERLFASFMENLPGLAWIKDTGGRYVYLNGAFERAFHVKLS
                     DWKGKNDFDVWPQAV"
BASE COUNT          856 a         1161 c         1101 g          716 t
ORIGIN      
        1 atcggcaaag ccgcgtccct ggcgaatcgt gtacggtcct attttcttaa gggcggcgat
       61 cactcgccta aaacgacgct gctggtcagc cagatcgcgg acctcgaaac catcgtcacc
      121 cgatcggaat tggaagcgct catcctagaa agcaatctga tcaagcgcca tcggcccaaa
      181 ctcaacgtcg tgcttcgaga cgataaacaa tatccctacc ttcgcttgcc catcaaagag
      241 gatttcccgc gcctttcgat cgtccgccgc gtgcagaagg acggcgcgct ctattacggc
      301 ccctataccc cggccggggc attgcgcgag acgctgaaag tcatcaagag ggtcttccct
      361 ctcgcaacgt gcacgatcga gatcgacggc acggcggaac gggcctgcat cgaatttgag
      421 atcaagcgat gcatggcccc ctgcactggc aatcaatcca aagaggacta tcagaagatc
      481 gtcacgcagg tccgacagtt tctcgaagga cgcgaccgtg aattgctcga cgatctgcgc
      541 gcccagatgg ccgctgcagc ggagcgggag gaattcgaag aggccgcgcg attgcgcgac
      601 cgcctcttca agattgaacg cacgctggag aaacaacggg tcacacaaac caccgccatc
      661 gatcaggacg tgatcggtgt cgcgcgacag ggaacagccg tcgatctcca gttgctgttt
      721 gtccgtggcg gtctcttgat cggccgaaag gacttctttt ggcctcaggc aaccgaggcc
      781 ggcgatgaag aactcgttcg atcggtgatc gagcagttct acaacaaaga cgggcagccg
      841 cccaaggaac tgttgctccc tgtcgagctc ggcgactcga cacttattga agagtggctc
      901 tcggagaaac ggggagataa ggtccgcctc gtggcgccgg aacggggggt gaaacaccaa
      961 ctgctcttgc tcgcggagga aaatgccacg gcttcggtcg ccgatcatct cagagacgag
     1021 ctgctggacc ggcaggctgg ggaagaactt cagcgcctgt tgcggctcga tcgacctccg
     1081 gtgcgcattg aaggcttcga catttcgaac acgatgggga accaatctgt cgcatcgatg
     1141 gtcgtctggg aacggggcca aatgaagaaa tcggactacc ggcgattcaa gatccagacg
     1201 gtcaccggcg ccaacgactt cgccagcatg caggaggtgg tgttacgacg ctaccgagac
     1261 agcgaagacc tcgcccgacc tgacctcgtg ctcatcgacg gagggctggg acaattggcg
     1321 gctgcgaggg agggactgaa acaggcagga gaaccggcca tggccgtcat cggtctcgcg
     1381 aaggcccgcg gcgagaaaga cgaacgaatt ttcctgccgg gccgaaaaaa ccccattctg
     1441 ctgcgggcga actcaccggc cactcatctc gttcaacgca ttcgagatga agcccatcgg
     1501 tttgccctga gcttccaccg aaggctacga agcaaggccc tcctcgcctc ccaattagat
     1561 cacatcatcg gaattgggga aattcgtcgc acctcgctgc tgagacgatt caaaacgctc
     1621 gagggcctcg cggctgcctc ggacgaagac ctgcgggcaa caggtttgaa ttcgcagacc
     1681 attcaggacc tccgcacgca gctgggaacg aagactacta cccgatccta accttccacc
     1741 ctttccagcg gtcactccat ctggctgtcc ttaagacgtt cttcgcccta gcaattcagc
     1801 agggaatggt tctatcctat cccccggttg ggggtggtaa ttcacccggg accgctcgca
     1861 gcagcgcgag cggctgcaca gaggctatcg actccgcatc gtcgcgagga gggtctcatg
     1921 gatgccccga tcgatacgtc acaccagacg tcggacgacg tcatcagctt gcggcagcgc
     1981 atcgccgaac ttgagcagac cattgcgcaa cagcgactgg cagatgcggc gttgcgcgaa
     2041 cgtcaggttc attttcagac tctctacgaa gataccccgg cgatggcgtt cgttctgtcc
     2101 atggacggaa cgattctctc tctcaatcgc tacggcgccg aacagttggg ctatactctc
     2161 gaggagttgg tcggccaatc cgtcttgcaa atttttgatc ccgccgatca tcacgccgtc
     2221 ctcaggcaac tgactgtctg tggacacact cccaacaaca ccctgggctg ggagttgcag
     2281 aaggtccgga aggacggctc ccgcctctgg gttcgggaaa cggcacggac cattcgagac
     2341 gcagcgggat cgccgactat cctcgtcgtc tgcgaagaca tcaccacgcg caagaccgcc
     2401 gaagaggagt tgcgcgtcac ggctcagaaa ctccaagcgc tgattcatgc ttgcccgctc
     2461 gctgtcctgt cgctggacaa cgaaggcgaa acggtgacgc tctggaatcc tgccgctgaa
     2521 gccatgtttg gttggcacgc ggacgaaacg ttgggccgac cgaccccgtt tctgcctccc
     2581 tcgcagcaca aagaatcggg ccggatgtgg gacgagctga tcaccagtgg ggcggtatcc
     2641 ggtgtggagt tccgccgggt ccgcaaagat gggaccccga ttgacgtctc cttgtgggca
     2701 accgtgctca aggacgaacg cgggatcgtc actgacaccc tcggcttcct agccgatatc
     2761 accgcccgca agcaggtcga ggcctccttg cgagagagcg aacaggccat ccgcacgttg
     2821 caggaagcga tttctcgtcc cacactgaca ttcgaccagc gcatccaggc gatgctggaa
     2881 ttgggttgtc gccgctttaa gcttccgacc ggactcatga cccgcgttca gggcaaggat
     2941 ctcgtcatca cacatgtctc gtctcaggac gcgaacatca ccgccgccat ggttcttcct
     3001 ctcgaccaga catactgtca cgccacactc atggcagagg ggccggtctg cttcgagcaa
     3061 gcgtcggcct cacaatggcg agatcatccg ggccatgcga cgctgggcat cgagtgttac
     3121 atcggcacca aactggtcgg actcaactca atccatggaa cgatctgttt cgtcgctccc
     3181 actccgtacg ccgcccgttt caccgatgca gacaaggact ttctgttgct catggcgcaa
     3241 tggatcggcc acgagcagga ccgccaggaa tcggagcggg ctctcaaacg acacgagagt
     3301 ctgttgcgcg ccgtcatcga caccgccacc gacgcgattt ttatgaaaga cacgaagggc
     3361 cgataccagc tcatcaatgc ggctggcgca cgaacaatcg gtaaaccggt ggaggagatc
     3421 gtcgggaaaa ccgacctcga attgttcccg cccgagacgg caaaacagct catggagaat
     3481 gaccagcggg tgctgctcgg cggaagccaa catcactttg aggcggttat cccattccag
     3541 ggagaagcca gaacgttttc atccatcaag acgccgcatc gcgaccctca agggaacgtg
     3601 atcgggctcg tcggtgtctc acgcgatatc accgatcaaa aactgctcga agagcagcgg
     3661 agcaagagcg agcgattgtt tgcctcgttc atggaaaatc tgcccggtct tgcctggatc
     3721 aaagatacgg gcggacggta tgtgtacctg aacggcgcgt tcgagcgagc ctttcacgtc
     3781 aagctgtcgg attggaaagg aaagaacgat ttcgacgtct ggccacaggc cgtc
//
LOCUS       DATDNW010000247         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_914666, whole genome shotgun sequence.
ACCESSION   DATDNW010000247 DATDNW010000000
VERSION     DATDNW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914666"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VI359_06025"
     CDS             complement(<1..1161)
                     /locus_tag="VI359_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY6973843.1"
                     /translation="MHKTLEEQLTLLSFLVVVLTVIASSLVQAGLSGTAAQGKPCLSP
                     HKQKTVMEKTLRAIVESHGQWLEQRYNHDYHRADLCQADLHQAALNGANLERANLEGA
                     NLRQANLYQSTLTHARLAGADLTKAGLEDSNLSGADMRQARLSHANLFRVIGDEAALF
                     NAVLSGTQLHESTFERTHFEGADLTSADFTNASVVDSYFYGANLHRATLTEADFMGTD
                     LRQTNLTKANLTKTNLQGALLDDAQLHGARLVEADLEGAYFDEADLTEASLRGALLRG
                     ADLRFTNLQSAGFQQADLEGVNFEGARFVKTNAQSANMTMAILYHAVVDEADFRNARL
                     HNAVLIGARGTGTIFSKTDLSDIYAPKVSLQRASFNDAKLELANLVAADLRKS"
     gene            complement(1206..1568)
                     /locus_tag="VI359_06030"
     CDS             complement(1206..1568)
                     /locus_tag="VI359_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY6973844.1"
                     /translation="MRLLWMVGFLAVVATFMRPLVIPAQEYPPDVTRGKAVYERHCVS
                     CHGTGGWGDGPNAPLLKVAPANFHRFSSFLKSDEELLRTIEHGVVYSPMHSWRGQLTD
                     GEMQDVVAYIRLLSQQGR"
     gene            complement(1577..1741)
                     /locus_tag="VI359_06035"
     CDS             complement(1577..1741)
                     /locus_tag="VI359_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY6973845.1"
                     /translation="MPMYDYKCQDCGKESLLVLTLKEHEAGKITCPSCQSTNVKQMYT
                     SFIAHTTKKS"
     gene            complement(1767..>2658)
                     /locus_tag="VI359_06040"
     CDS             complement(1767..>2658)
                     /locus_tag="VI359_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6973846.1"
                     /translation="ARRRPKRGAVMKILLAVDGSDYSYEAVRALKYLRRADQLILLHV
                     LNVPRPAYPMMMPEVADELYRAAERSMREDGERLLERVRSLLPPHTGPSTTRLELGSP
                     ADMIVELAKREQVDLIVMGARGVGPIKERLLGSVAHRVLTYASCAKLIVTTPLRALSR
                     LLLPIAGEADANHALQFLKLNPFASPVELRLLTVLPHTLPPWPAGSAAFELEATALRS
                     AREFIDGVGVQLRSLGYQSTAEAVLGSPVQAIIEECRKTSPDLILMGSRGSRGLGRLV
                     LGSVSHALLHHSPFPLLVFS"
BASE COUNT          542 a          782 c          770 g          564 t
ORIGIN      
        1 ggattttcgg aggtccgccg ccacgagatt ggccaattcc aatttggcgt cgttgaagga
       61 tgcgcgttgc agcgacacct tcggcgcgta gatatcgctc agatccgtct tggagaagat
      121 ggttcctgtg cctcgcgcac cgatcagaac ggcgttgtgc aatcgggcat tgcggaaatc
      181 ggcctcgtcg accaccgcgt ggtacaaaat agccatcgtc atattggcgg attgggcatt
      241 ggtcttcacg aatcgcgcgc cttcgaaatt gacaccctcc agatcggcct gctgaaaacc
      301 cgcggattgc aaattggtga agcggagatc cgctccccgg agcagcgctc cgcggagact
      361 ggcttccgtc agatcggctt cgtcgaaata tgccccttcc agatccgctt ccacgaggcg
      421 cgcgccgtgg agttgtgcgt cgtcgagcaa cgctccttga aggttcgtct tcgtcaggtt
      481 ggccttggtg aggttcgttt ggcggagatc ggttcccatg aagtccgcct cagtgagcgt
      541 cgcccgatga agattcgccc cgtaaaaata actgtcgacg acgctggcat tcgtgaaatc
      601 ggctgaggtg agatcggcac cttcgaaatg ggtccgttcg aaggtcgatt cgtgcaactg
      661 ggtcccgctc aagacggcgt tgaagagcgc cgcctcatcc ccgatcacgc gaaacagatt
      721 cgcatgcgac aggcgggctt gccgcatatc ggctccggac agattgctgt cttcgaggcc
      781 ggccttggtg aggtctgctc ccgccagacg tgcatgcgtc aaggtgctct ggtacaaatt
      841 ggcttgccgg agattggctc cctccaagtt cgctcgctcc aaattcgctc cgttgagcgc
      901 agcctgatga agatcggctt ggcagaggtc tgcacggtga tagtcatggt tgtatcgctg
      961 ttccaaccac tgaccgtgcg attcgacgat cgctctcaat gtcttctcca tgacggtttt
     1021 ctgcttgtgg ggactgaggc agggtttgcc ctgcgcggcc gtgccggata gtcccgcttg
     1081 cacgagagag cttgcgatca ccgtcaatac gacaaccaag aaggaaagca gggtcaattg
     1141 ttcttcgagc gtcttatgca ttgaacttct ccatcatgat gattcctcgg tcgctccagg
     1201 gacggctagc gcccctgctg tgacaataag cgaatatagg cgaccacgtc ttgcatctcg
     1261 ccgtcagtga gctgcccacg ccacgaatgc atcggactgt agacgacacc gtgctcaatg
     1321 gtgcggagca attcttcatc cgatttcaga aacgaactga agcgatggaa attggccggc
     1381 gcaactttca gcaatggggc attcggaccg tcgccccatc ctccggtacc atggcaggaa
     1441 acacaatgac gctcatacac ggccttcccg cgcgtcacat cgggaggata ctcctgcgcc
     1501 ggaatcacga gaggtctcat gaaggtcgca acaaccgcca gaaatcccac catccataac
     1561 agacgcatgc gtgccctcaa ctcttcttgg tggtgtgggc aataaaggac gtgtacatct
     1621 gcttcacgtt cgtgctctgg caagaaggac aggtgatttt ccctgcctcg tgctctttta
     1681 acgtcaggac gagcaacgat tcctttccgc aatcctgaca cttgtagtca tacatcggca
     1741 taaagactcc ccctctcgac ggccttctag ctaaaaacca acagagggaa gggactatgg
     1801 tgcaacagtg cgtgggagac acttcccagt acgaggcgcc caagtcctcg gctcccgcga
     1861 gagcccatca gaattaagtc cgggctcgtc ttccggcact cctcaatgat ggcctggacg
     1921 ggtgagccca acacggcctc cgcggtcgac tgatacccga gcgaacgcag ctgcacaccg
     1981 actccgtcga tgaactcacg cgcgctccga agcgcggtcg cttccaattc gaacgctgcc
     2041 gaccctgccg gccaaggcgg taacgtgtgg ggcaggacgg tcagcagtct gagctcgact
     2101 ggtgatgcaa aggggttgag tttgagaaac tgcagggcat ggtttgcgtc cgcctcgcct
     2161 gcgatgggaa ggagcaggcg cgaaagggct cgtaggggag tcgtgacgat caatttcgcg
     2221 caggaggcat aggtcagcac gcggtgagcg acgcttccga gtaaccgctc tttgatcgga
     2281 ccaactccgc gtgctcccat gacgatgagg tcgacttgtt cacgcttcgc cagttctacg
     2341 atcatgtcgg ctggcgaccc cagttccagt cgcgttgtcg aggggccggt gtgcgggggc
     2401 agcaacgacc gcacgcgctc gagcagccgt tcgccgtctt cccgcatgct ccgttcggct
     2461 gcacggtaca gttcgtcggc gacctccggc atcatcatgg ggtaggccgg gcgcggcacg
     2521 ttcaagacat gcagcagaat caactgatcc gccctcctca gatatttcaa cgcacgaacc
     2581 gcttcgtagg agtaatcgga cccatcgacg gcgagcagga ttttcatgac ggcacctctc
     2641 ttaggtctcc ttcgagcg
//
LOCUS       DATDNW010000248         3551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_914733, whole genome shotgun sequence.
ACCESSION   DATDNW010000248 DATDNW010000000
VERSION     DATDNW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914733"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /gene="hisH"
                     /locus_tag="VI359_06045"
     CDS             <1..295
                     /gene="hisH"
                     /locus_tag="VI359_06045"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY6973847.1"
                     /translation="VDKHEGRGLKIPHMGWNQANFQRDCPLFDGIAEGANWYFVHSYF
                     VDPSDRQIAATTTTYGIPFVSSIWRDNVLGCQFHPEKSQTVGLQLIKNFGSWK"
     gene            286..1029
                     /locus_tag="VI359_06050"
     CDS             286..1029
                     /locus_tag="VI359_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973848.1"
                     /translation="MEVSLVSQRRWSAAVVGLAVLAAIGCSGQKVTTKSSSELTRHPV
                     RTLALVPFTTLNTPQVRELGDPFFATPQSVRRSDISVAVPSNVEPPSRQTVTVPAYAA
                     EKITQLFWTRLRMREGVTVSPASESTKASPTNVDVSKATQEAVGAAIAARLKADAALV
                     GQVLVFQERVGSRLGADPPAAVGFEVKVVASDETVLWIGNYYERQRPMTEDMLGFLQR
                     WGAFVTAEELAQYGVDEILKEFPFGSSGK"
     gene            1053..1772
                     /gene="hisA"
                     /locus_tag="VI359_06055"
     CDS             1053..1772
                     /gene="hisA"
                     /locus_tag="VI359_06055"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY6973849.1"
                     /translation="MIVIPAIDLKDGRCVRLRQGDMAAETVYSSDVPAMARRWQEQGA
                     RLIHVVDLNGAVDGEPRNLSEIQSVVKTVSVKVQVGGGIRNIDTVRRYLAAGVSRVVL
                     GTAALTDRMLLEQACKEFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLKELSGYEL
                     GAVIYTDIARDGMLGGPNIHALREVVERSSLPVIASGGITRLEDLRAIRSLGPRVEGV
                     IVGKALYDGELDYAAAVVAVG"
     gene            1883..2644
                     /gene="hisF"
                     /locus_tag="VI359_06060"
     CDS             1883..2644
                     /gene="hisF"
                     /locus_tag="VI359_06060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY6973850.1"
                     /translation="MLTKRIIPCLDVTGGRVVKGVSFVNLRDAGDPVEVATIYDREGA
                     DELCFLDITASHENRKTIIDVVERTADRVFMPLTVGGGVRTLDDIRALLNAGADKVSI
                     NTAAVERPEFVKEAAERFGTQCIVVAVDAKRRASPAAGWEVFTHGGRKPTGIDVVEWA
                     MRMERYGAGEILLTSMDQDGQQTGYDLALTAAVSETVSIPVIASGGVGTLAHLYDGFV
                     TGKADAVLAASIFHYRTHTIPEAKAYLRDKGVAVR"
     gene            2644..3297
                     /gene="hisIE"
                     /locus_tag="VI359_06065"
     CDS             2644..3297
                     /gene="hisIE"
                     /locus_tag="VI359_06065"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY6973851.1"
                     /translation="MDLTQLKFDAQGLIPVVVQDWRDGTVLMVAFMNKEALHKTVTTK
                     SVHFWSRSRQTLWEKGETSGHKLVLKDLFVDCDGDALLVKAEPIGPTCHTGEKACFFS
                     RIDEPGAKTSEAWGGILERLYQTIQDRKRQPSSDSYTSKLLDGGVDRVLKKVVEEAGE
                     VVLAGKGGKKDEIIYETADLLFHTLVALGYHDITLQEVYQELATRFGKPGLRTGAAS"
     gene            3294..>3551
                     /locus_tag="VI359_06070"
     CDS             3294..>3551
                     /locus_tag="VI359_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY6973852.1"
                     /translation="MSDCIFCKIANKSIPSKLIYEDDQVVAFDDLHPQTPVHSLVIPK
                     RHVVSVQELGEQDQALLGRLVLACTKIAKLKGLAENGYRLVT"
BASE COUNT          765 a          878 c         1157 g          751 t
ORIGIN      
        1 ggtcgacaag cacgaaggca gaggtctaaa aatcccacat atgggctgga atcaggctaa
       61 tttccagcgg gactgtccgt tgttcgacgg gattgcagag ggcgccaatt ggtatttcgt
      121 acattcttat ttcgtagatc ccagtgatcg gcagatcgct gcgacaacga cgacctacgg
      181 cattccgttt gtgtcaagca tctggcggga caatgtgttg ggttgccaat tccatccgga
      241 aaagagtcaa actgtcggat tgcagctcat caagaacttt ggatcatgga agtaagtctg
      301 gtgagtcaga gacgatggtc ggcagcggtg gtcgggctgg ctgtgttggc agcgattgga
      361 tgtagcggcc agaaagtcac cacgaagtcg tcatcggaat tgactcggca tccggttcgc
      421 acattggcgc tggtcccctt cacgacactg aacacgccgc aagtacgaga gctcggcgat
      481 ccgttcttcg cgacaccgca gagcgtgcgc cggtccgata tatccgtggc ggtgccgtcg
      541 aatgtagagc cgccatcgcg gcaaaccgtc accgtgccgg cctatgcggc ggagaagatc
      601 acgcaactct tctggaccag gctgcggatg agagaaggcg tcacggtctc gcccgcgagc
      661 gagtcgacga aagcatcgcc caccaacgtg gatgtgagca aggcgacgca ggaggcggtc
      721 ggggccgcca ttgcggcacg actcaaggca gatgccgcat tagtcggcca ggtgctcgta
      781 tttcaagagc gcgtgggcag ccgcctgggc gccgatcctc cagccgctgt gggatttgaa
      841 gtgaaagtcg tcgcgtccga tgagacggtc ctctggattg gcaattacta tgagcgccag
      901 cggcctatga cggaagatat gttggggttt ctccagcggt ggggtgcctt cgtcacggcg
      961 gaggagcttg ctcaatatgg tgtcgacgaa atcttgaagg aattcccgtt cggttcttcc
     1021 ggcaagtaat ctaatcgaaa ctggttccag gcatgatcgt tattccagca attgatttaa
     1081 aagatgggcg ttgtgtgcga ctccgtcaag gcgatatggc agcggagacg gtctattcca
     1141 gcgatgtgcc tgcaatggcc cgccggtggc aggagcaggg ggcacggttg atccatgtcg
     1201 tcgatctgaa cggtgccgtg gacggcgagc cgcgcaatct ttctgagatt cagtccgtcg
     1261 tcaagaccgt aagtgtaaag gtgcaggtgg gtgggggcat cagaaatatc gatacggtgc
     1321 gtcggtacct cgccgcgggg gtctcccgtg tggtgctggg caccgcagcg ttgacggacc
     1381 ggatgttatt ggaacaagca tgcaaggaat tccctcgccg cattctgctt ggactcgatg
     1441 cccgcgatgg gaaagtggcg gtcaagggtt ggacggcggt atcggaaacc aaggcgatcg
     1501 acttattaaa agaattgtca ggctacgagt tgggcgcggt catctatacc gacatcgccc
     1561 gtgatggcat gctgggtggg ccgaacatcc acgcattgcg cgaagtcgtg gagcgatcgt
     1621 ccctccccgt cattgcgtcg ggtggcatta cgcgcctaga agacctgcgc gcaattcggt
     1681 cgcttgggcc ccgggtcgag ggggtgattg tggggaaggc tctttacgac ggggaacttg
     1741 actatgccgc tgcggtggta gcggtgggct agaggcaaga ggcgaggggc gagaggtgat
     1801 gagaggaaat tgtttcgagg ggaggagagg ctcttgccac tagccccttg ccccatgcca
     1861 tgagcgagtg gagcgagcga gtatgttgac caagcgcatc attccctgcc tcgatgtgac
     1921 aggtggccgg gtggtcaagg gtgtgagttt cgtcaacttg cgcgacgcgg gtgatccggt
     1981 cgaagtggcg acgatctatg atcgtgaagg ggcggatgaa ctctgttttc tcgacattac
     2041 ggcctctcat gaaaaccgga agacaataat cgacgtggta gagcggacag ccgatcgagt
     2101 gttcatgccg ctgacggttg gcggaggagt gcgcacgctg gatgacatcc gagcgctgtt
     2161 gaatgccggg gcagacaagg tcagcattaa cactgcagcg gtcgagcggc cggaattcgt
     2221 caaggaagcg gcggagcgat tcgggacgca gtgcattgtt gtggccgtcg acgcaaaacg
     2281 tcgtgcctct ccggctgccg gttgggaggt attcacgcac ggaggtcgaa aaccgactgg
     2341 aatcgacgtt gtggagtggg caatgagaat ggagcgatac ggcgcaggcg agattctatt
     2401 gaccagcatg gatcaggatg ggcagcaaac cggctacgat ctggctttaa cggcagccgt
     2461 atccgaaacg gtctcgattc cggtgattgc ctcgggcggt gtcggcacgt tggctcattt
     2521 gtatgatggc tttgtgaccg ggaaggccga tgcggtcttg gccgcgtcga ttttccacta
     2581 ccgaacccac acgattcctg aagccaaagc gtatcttcgt gacaaagggg tcgcagttcg
     2641 ataatggatc tcactcaact gaaatttgat gcacagggtt tgattccagt cgtagtgcag
     2701 gattggcgtg atggcacagt gctgatggtg gccttcatga acaaggaggc gctgcacaaa
     2761 acagtcacga ccaagtccgt gcatttctgg agccgatctc gtcagacact ctgggagaag
     2821 ggcgagacct ctggccacaa gctcgtactc aaggatctct ttgtggattg tgatggtgac
     2881 gctctgctgg tgaaggccga gccaatcggg ccgacctgcc atacgggcga gaaagcgtgc
     2941 ttcttctctc gtattgatga gccgggggcg aaaacctctg aggcctgggg cggcattctc
     3001 gagcggcttt atcagacgat ccaagatcgc aaacgacaac catccagtga ctcctataca
     3061 tccaaactct tggacggcgg cgtcgatcgg gtgctgaaga aggtcgtgga agaagccgga
     3121 gaagtggttt tggcaggaaa gggcggcaag aaagatgaga tcatctatga gacggcggat
     3181 cttctcttcc acacgttagt ggctctcggc tatcatgaca ttacgctcca agaggtctat
     3241 caggaattgg cgactcggtt cggtaagccg ggcttgagaa caggggccgc ttcatgagcg
     3301 actgcatttt ttgcaagatt gccaacaagt cgattccgtc gaagctgatc tacgaagacg
     3361 atcaggtcgt agcattcgac gatctccatc ctcagacccc cgtccactcc ctggtcattc
     3421 ccaagcgaca tgtggtgtcg gtgcaggaat tgggggaaca ggatcaggcg ttgctgggac
     3481 ggctggtgct ggcctgtacg aaaatcgcta aactcaaggg gctggccgag aacggctacc
     3541 ggttggtgac g
//
LOCUS       DATDNW010000249         4777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1193735, whole genome shotgun sequence.
ACCESSION   DATDNW010000249 DATDNW010000000
VERSION     DATDNW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193735"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VI359_06075"
     CDS             <1..224
                     /locus_tag="VI359_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973853.1"
                     /translation="EMKPIPSTFNYDHVTGFSQEVREKLKKIRPASVGQASRISGVTP
                     AAISLLLVALSKYRGASPKDFITPVIASY"
     gene            293..946
                     /gene="rsmG"
                     /locus_tag="VI359_06080"
     CDS             293..946
                     /gene="rsmG"
                     /locus_tag="VI359_06080"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY6973854.1"
                     /translation="MEHQEDLRKFMAAGAATMGVSLAPTHVDQFMTYLQELIRWNKKI
                     NLTGIDNPRDIIVKHFLDSLAALNACTIPLRSEIVDIGSGAGFPGIPLKIVRGDLQLM
                     VVEPVHKKTSFLKSLIGLLKLKDVSTFDGTVQEYVKMPRKTLADFVVVRAIKLNEIMP
                     SMTELLRLGGRALLYRTESIDSTEIYSNFTLIKEVSFTLPHGYGHRVISVMEMRKAA"
     gene            1024..1800
                     /locus_tag="VI359_06085"
     CDS             1024..1800
                     /locus_tag="VI359_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY6973855.1"
                     /translation="MAKIIAVANQKGGVGKTTTAVNLAAGIALQGKRVLLVDIDPQAN
                     ATSGLGIDPRSLQTTVYNCLINYVKFVDAVQATAVQGLSLLPSNAELAGAEVELVNVE
                     EREKVLRHFLKDVEPQFDFVFLDCPPALGILTLNALVAAQSILIPVQCEYYAMEGLGR
                     LMGSIDRVRQSFNPGLEVEGIVLTMYDARNTLARQVVEQVREYFRDRVYQTMIPRNVT
                     LAEAPSYGRPVYLYNMASAGSQSYLSLAKEFISHGEKSAR"
     gene            1778..2626
                     /locus_tag="VI359_06090"
     CDS             1778..2626
                     /locus_tag="VI359_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY6973856.1"
                     /translation="MEKKALGRGLDALIPTSKPAPMPELPEVQHLRTDAIVPNRYQPR
                     QTFSPQELAELTASLKQSGLLQPILVRRKGDGMYELISGERRWRAAKQAGMETIQAII
                     RNCTDEESIVLALIENLQRQDLNPIDAAKAYHRMMNEFGLTQDIIAQRVGCERSSIAN
                     TVRLLNLPVDVQRLIESGELSTGHAKVILGLTNSEAQLRFAELVVSRGLSVRELEKLI
                     QFSTLGRKRAKLEPRRSPWSEVEERLRKRLGTKVTILRGRRGGKVIIHYFSPPELDGI
                     LETLLS"
     gene            2791..3267
                     /locus_tag="VI359_06095"
     CDS             2791..3267
                     /locus_tag="VI359_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6973857.1"
                     /translation="MWGQEKKPTDQVSEGEGNAEGTSEKPAQEAQDISAFVGKGVEFK
                     GTISYSGTVRIDGYLDGEIHTDGVLLIGEDAVLQAKITAGTVVCKGKITGDIAVKEQV
                     KLRAPAVVNGSVKTPLLSMEDGVLFNGNLEMAQQVPQPSLHSVGMITSTGVKRVNG"
     gene            3361..3786
                     /locus_tag="VI359_06100"
     CDS             3361..3786
                     /locus_tag="VI359_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY6973858.1"
                     /translation="MWAMKERNPEVDGLNENFTLLGRGVDFKGIVSFDGTVRVDGRVE
                     GEIHTTGTLIVGEHAVIKGVVSAGVLMNSGKINGTITANEKIQILKPGILVGDIRTPL
                     IAIEEGSHFHGMCDMGATQWREELPVPIESQSTLSIAAH"
     gene            3882..>4777
                     /gene="mnmA"
                     /locus_tag="VI359_06105"
     CDS             3882..>4777
                     /gene="mnmA"
                     /locus_tag="VI359_06105"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY6973859.1"
                     /translation="MDSSVAAALLVRQGYDVHGVTLQVWEHEDETVAPSKRWQERGCC
                     KIGIAKFVAQKLQIPYEVIDTRQTFRTGVIDDFLDGYLQGITPNPCVRCNERVKLRSL
                     IELARERDIDYVATGHYIRVATWHSGRIPRKAADAKKDQSYFLYRLNPSWLPQLLFPV
                     GEMQKSDVWREAEALGLPADELKESQEICFVSHGDYRTFIEKEAPASRKPGLFVDADG
                     NQLGEHDGIAFYTPGQRRGLGLAVGERLYVQKVISETNTVVLGPPASLLRDTCHVMEL
                     NLFDPCLMTESRDVQVKVRYATP"
BASE COUNT         1219 a         1205 c         1240 g         1113 t
ORIGIN      
        1 tagaaatgaa accgattcca tcaacattca attacgatca tgtgacaggc ttctctcaag
       61 aggttcgaga gaagctgaaa aaaattcgtc cagcctcggt tgggcaagcc tcgcgaatct
      121 caggtgtcac gcccgccgca atctcattac ttcttgttgc tcttagcaag tatcgaggag
      181 cgtcccccaa agacttcatc acccccgtca tagcatcgta ctagagacac agaaagcgtc
      241 cctttacacc tacacccttg acctgctctg ccgaataacg taattgttcc acgtggaaca
      301 tcaggaagac cttcgaaaat tcatggctgc gggcgcggca acgatgggtg tatcacttgc
      361 cccaacccat gtcgatcaat tcatgacgta ccttcaagag ttgatacggt ggaataagaa
      421 gatcaatctc actggcatcg acaacccaag ggacattatc gtcaaacatt tcctcgactc
      481 attggctgcg ttgaatgctt gtactatccc gctcaggagc gagatagtgg acataggctc
      541 aggagctgga tttccgggaa ttcctctcaa gatcgtcaga ggggatcttc agctgatggt
      601 ggttgagcca gttcacaaaa aaacctcatt cttaaaatcg cttataggtc tcctcaagct
      661 caaggatgtt tccacttttg atggaactgt tcaggagtat gtaaagatgc caaggaagac
      721 actcgcagac tttgtcgttg tcagagccat taaactcaac gaaatcatgc cctcaatgac
      781 ggagctgctt cgtctgggag ggcgagccct actctatcga accgagtcta ttgactctac
      841 tgaaatttat agcaatttta ctcttattaa ggaagtgtcc ttcactctcc cccacgggta
      901 tggccatcga gttatctcgg ttatggagat gagaaaagct gcatgatgag tgagcatatc
      961 tcatcaatgt tccacgtgga acattgatcg agtgtctata accttccatt cacattaata
     1021 ggtatggcaa agatcattgc agtggcaaat cagaaaggtg gggtgggaaa aacaacaacg
     1081 gccgttaatt tggctgctgg aattgcgctc caagggaagc gggttcttct cgtggacata
     1141 gatccgcagg cgaatgctac aagcggtctt gggattgatc cgcggtcctt acagacaacg
     1201 gtctataact gccttataaa ttatgtgaaa tttgtcgatg ccgttcaagc gaccgccgta
     1261 caaggcctct cattgttgcc atcgaatgcc gagctggccg gtgcagaagt tgagttggtt
     1321 aacgtcgaag agcgagaaaa agtccttcgt cattttctga aagatgtcga accgcagttt
     1381 gacttcgttt ttctcgattg tcctcccgct cttggcattt taaccctcaa tgcgttggtt
     1441 gcggcccaat cgattctgat tccagttcaa tgcgaatatt atgcaatgga gggattaggg
     1501 cgactgatgg gaagtatcga tcgcgtccgc caatcattca atccaggtct cgaagtcgaa
     1561 ggcattgtat tgacaatgta tgacgcgagg aatactttgg cgcgtcaggt ggtcgagcaa
     1621 gtgcgtgaat atttcagaga ccgtgtctat cagacaatga tcccacgtaa cgtgaccctt
     1681 gcagaagcac cgagctacgg gcgtcctgtc tatctataca acatggcatc agcgggatct
     1741 caatcctatc tctctctagc aaaggagttt atcagccatg gagaaaaaag cgctcggtag
     1801 aggactcgac gcactaatcc ctacatcgaa gcccgctcct atgccggaat tgccagaggt
     1861 gcaacatctc cgcacggacg ccatcgtgcc taatcgctat cagcctcgac agacgttttc
     1921 tccccaagaa ttggcagagc tcacggcgtc gttgaaacaa agcggtcttc tgcagccgat
     1981 tcttgtccgg cggaaaggag atgggatgta tgagctcatc tctggtgagc gacgctggcg
     2041 tgcagcaaaa caagctggca tggaaaccat tcaggcgatc atcagaaact gtaccgacga
     2101 ggaatcgatc gttctggcac tcatcgaaaa tttgcagcgg caagatttga atcctataga
     2161 cgcagcgaag gcttatcacc gaatgatgaa cgaatttgga ctcacgcagg acattattgc
     2221 gcagcgcgtt ggatgtgaac gatcatcgat tgcaaacaca gtccgcctgc tcaatctccc
     2281 ggttgatgtt caacgtctca ttgaatccgg cgaattgtcg accggccacg ccaaggtcat
     2341 ccttggactc acaaattcag aggctcaact cagatttgcc gagctcgttg tgtctagagg
     2401 cctttcagtg agggagctag aaaaactcat tcaattttca acacttggaa gaaagcgtgc
     2461 caagctagaa ccccgacgat caccctggtc tgaggtcgaa gaacgcctga gaaaacgatt
     2521 gggaacaaaa gtcacaattc tcagagggcg tcgcggaggg aaagtcatca tccactattt
     2581 ttcaccgcct gaattagacg ggattctcga aacactcctc agttaatcca caggccattt
     2641 tcctccgcag atcgtacttc cacgtagagg catatttgtt gcttgcccct agtgaccagt
     2701 acggtgtttg cctacaccaa ccgattctaa actccaccag ggaataggtt ccgaacaagc
     2761 cgaatcgcac attcgtagga ggcgtttttt atgtgggggc aggaaaagaa gccgaccgac
     2821 caagtcagcg agggagaagg taatgctgag ggcacatcag agaaaccggc tcaagaggct
     2881 caagatatta gcgcctttgt cggcaagggt gtggaattca aaggcacaat ttcatatagc
     2941 gggacggtcc gtatcgatgg atatcttgat ggagaaatcc acacggacgg cgttctattg
     3001 atcggcgagg atgcagtcct tcaggcaaaa atcactgcag gaacggttgt ctgcaaaggg
     3061 aagatcaccg gagatatcgc cgtcaaagaa caggtcaaat tacgcgcccc cgccgtggtg
     3121 aacggttcgg tgaaaacccc actgctctcc atggaggatg gcgttctgtt caatggcaat
     3181 ttggaaatgg cccaacaagt ccctcaaccc tcgctccatt cggttggcat gattacatca
     3241 accggggtga aacgggtcaa cggctaaggg gtgaagggca atttgtacgt aagtatttat
     3301 tgatatcatc agccatagtc ctcgtgtcca ggtgactaag gggaaacaga ggagggtatc
     3361 atgtgggcga tgaaggagcg gaatccagag gttgatggat tgaatgaaaa tttcacgctg
     3421 ctgggtcgag gcgttgattt caaaggcatc gtgagtttcg acggaaccgt tcgcgtggat
     3481 ggccgtgtcg agggggaaat tcataccact ggtaccttga ttgtcggcga acatgcggtc
     3541 attaaagggg tcgtctcggc gggtgtcttg atgaatagtg ggaagatcaa cgggaccatc
     3601 acggcgaacg aaaaaattca gattctcaaa cccggcatcc tagtcggaga cattcgcacc
     3661 cccctgatcg ctatcgaaga agggtcccac ttccacggga tgtgtgacat gggagcgact
     3721 caatggcgag aagagttgcc cgttcccata gagagccagt cgactctctc gatcgcggca
     3781 cactagccag catattcagg cctctagccc ttcctcccga tccccggtac aatagcgtcc
     3841 ctatgactcg cccaactgtt ttactcggga tgagcggtgg tgtggatagc tctgtcgcag
     3901 cggcattgct cgttcgccag ggctatgatg tccacggtgt cactctccaa gtgtgggagc
     3961 atgaagacga aaccgtcgcc ccgtccaagc ggtggcaaga acggggctgc tgtaagattg
     4021 gtattgccaa gttcgtcgcg cagaaactcc agatccccta tgaggttatc gatacgaggc
     4081 agaccttccg caccggcgtg atcgacgatt ttctggacgg atacctgcag gggatcacgc
     4141 ccaatccctg tgtccgctgc aatgaacgtg tgaagcttcg cagtctgatt gaactggcac
     4201 gagaacgtga catcgactac gtggcgaccg gccactatat acgtgttgca acctggcaca
     4261 gtggtcgcat ccccagaaag gcagcggacg ccaaaaagga tcaaagttat tttctctatc
     4321 gtctgaatcc ctcatggctc cctcaactgc tcttcccggt cggagagatg cagaagtccg
     4381 acgtctggcg cgaagccgaa gccctaggct tgccggcgga tgaactcaag gagagtcagg
     4441 aaatttgctt cgtcagtcat ggcgactacc gcaccttcat cgagaaagaa gcccccgctt
     4501 ctcgaaagcc cggcctattt gtcgatgccg atggaaatca attgggggag cacgacggta
     4561 ttgcgttcta cacgccgggc cagcgccgtg gcttaggtct tgccgtcggc gagcgcttgt
     4621 atgttcagaa agtgatatcc gaaaccaata cggtcgtcct cgggccgccg gcaagtctcc
     4681 tgcgtgatac ctgccacgtc atggaactga atctgtttga cccctgtctc atgaccgaat
     4741 ctcgtgacgt acaagtgaaa gtccgctatg ccacgcc
//
LOCUS       DATDNW010000250         7043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_578813, whole genome shotgun sequence.
ACCESSION   DATDNW010000250 DATDNW010000000
VERSION     DATDNW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578813"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1797
                     /locus_tag="VI359_06110"
     CDS             97..1797
                     /locus_tag="VI359_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973860.1"
                     /translation="MTLVLLVPLLLLAAACIVVIGPGGSEPARATLAAYPIGAAFLGA
                     VATLYLVTTGGPVSVRFYNLSSVASFAIPIGFYVDRLSAVMMTLITGVSVIIYSYSTG
                     YMYQDRHARRYLAMICLTDFVLICMVSSANLMMLFLFWQVLSYLLYVLAHNHAHAGTL
                     AGAFKTFALLRVADTAFLAGIALAYHLYGTLEFQELFQRAADMPVMLSLWAGMDMSGT
                     TAVTLLLFVGAMGKSAQFPIHLWLPGSLFAPTPVHALLHAGIINAGGFLINRLAPLFG
                     LSSITLHVAFLIGTLTAVLGASMMLAQNDIKKTLGFSTIGQMGYMIMECGLGAFALAV
                     FHLIAHGLFKATVFLNCGNVIHKARQEPHFPYVDHHAEEPGLSGLTWFTGSVTTLFIP
                     LLILLVTHGVLRIPLLESHGTVIFLFFIWITSSQAILTLTRLRQVASWKVSAAMLMTL
                     LFIVFVYLFAVESFTEFLYPNPGEVASYFKAADLPAWLFDLIIVTATILTVAGWSYLY
                     MSAHGMTLRMPAWIDSMRIRLYLPFMNRLYADELYALTGQTIMRLVHQLDKRERGWSR
                     "
     gene            1794..3104
                     /locus_tag="VI359_06115"
     CDS             1794..3104
                     /locus_tag="VI359_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6973861.1"
                     /translation="MMDGTLIPWLLAGIPLLGVLASLPFRSDPERLKGSSVAWAVISL
                     GSVVGFAGRIDVPLEGLLPLYLLPLAATISILGQPAHKHHRRSWMLTLLFLGLGLSAL
                     SSERPISLLSVSLLLVLVAVLLYRHHSALWPRSWWGIGAYGLGALCAALSVAADAPLS
                     SVASLLACAVLLPLVPFHDGHLTALTRLPGSLPSFIVVLLPMAGLHQLASAMPTVPDI
                     VAWTVSLFALVGASYGALKALAQSRIRLLLAYGSLSFFSILWWFTAAFRMVTPRSALL
                     VGTVGLATSGLMVAWQVVRTRYGDDVDPQAISGLASGMPQYAVLLSLLALAAMGLPPF
                     GVFAGFMGLLLSSPFPSLPGLFVTLLAWLAASWYIMQMVQRLLFGVRRPELRYADLLR
                     AELASLLIVVLVLLALGLAPTTLFAPEGALFGTSTMGESPTWTR"
     gene            3092..6292
                     /locus_tag="VI359_06120"
     CDS             3092..6292
                     /locus_tag="VI359_06120"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEY6973862.1"
                     /translation="MDSLDRATDIEARRMELRGVVRLAGEVIAQYWPMRTFVHHNPLH
                     SLEYLPFEETVRRGKQFMGGSGYLPSTLYHHYLRSGRIRPEHLDQVLCHVADDKRVVI
                     GARTVTHGDVLRACLTQELVLPVAEPLDSQLPDPSHTLIEQIAARLESVLTYPDLSTR
                     IRAIVEGDQAALGQWITLSHWCDDTLGTQIVRQINDQLIKWCEAFLDEGHATWAMPKR
                     EKGLYQAWKMIAAHEWSPCGIADSGRKIAALPDHPEDALLESLDALGIPAELRQDYLS
                     LQLTALPGWAGFIKWRGEERDYRWQQAYPAGLVKFLAIRLWYARELVQKACREELGIE
                     GRYAAVTAYMRSRPEEYYLRRQRVAGRLPALYAEEVDLLAHQRGNGWKEVLHRYQIEV
                     VPRQAAAARRGAARTLLALAQSLAIDPEQLVGTAPETLKQIVHWVEAFPESAHGPVWL
                     KALEASYQEALLGQLRRPTAVPDGTDKSRPYSQSVYCIDVRSEPFRRHLESTGSHETY
                     GFAGFFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDHKVSKHEARARWVHA
                     GHTLLHDLKENVVTPYVMVESLGWFYGIPIFGKTLLPSLYRRWTAWLQRLFVPAIATT
                     LTVDKLAPADTAEMVAAEQQAVVRKALQERLGLRSSRMTPALVESLRQHALSGSEEPD
                     QPLTDAAALAGLSKDALVGLTEVLRRQYELNARAASRHKERITRTGFTLEEQTLTVDT
                     ALRMMGLTKNFARLVLFCAHGSTSDNNPYESALDCGACGGNEGKPNARVLAMMANNQQ
                     VRGRLAKNGIEIPSDTHFIAGQMDTTTDAVQLFDLEDVPPTHRADLARLQEDLAQAGE
                     LTSQERCARLPDVQQPIAGNKAKAHVRTRSTDWSQVRPEWGLSGNAAFIIARRELTKG
                     MDLGGRVFLHSYDYREDPTNRLLEVLLTAPQVVAQWINMEHYFSTTDNEVYGSGSKVY
                     HNVVGRLGIMSGPWSDLRLGLARQTVMNGEVPYHEPMRLLTIIEAPRERIDKLIGRHE
                     VLQHYYHNQWVHLVAFDPDDGVWYRYHSKGMWNVIP"
     gene            6349..6687
                     /locus_tag="VI359_06125"
     CDS             6349..6687
                     /locus_tag="VI359_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6973863.1"
                     /translation="MGLTLHPMKEIRVIVAGEHRSFVTDLLDRVNATGYTIIGNVSGK
                     GHHGVREAHFMFSEQESLEMIMTVVPEEKVEPILAGLRPLFERHSGVMFVGDVAVSRE
                     EYFGKKGKGA"
     gene            6786..>7043
                     /locus_tag="VI359_06130"
     CDS             6786..>7043
                     /locus_tag="VI359_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973864.1"
                     /translation="MKGLRFERIGQDRYYNVVFHMGSTYVPVSDETVEELKAQSLLPP
                     ERFLDLLIDRIGYSSYLKDQIRTELKSSGDPVTQITVLQGAI"
BASE COUNT         1264 a         2169 c         2122 g         1488 t
ORIGIN      
        1 gctttgatga gacggcgcag ggacggacac cctgatggcg cgcgcttgac ggcggtccgg
       61 gtgccgtatt aatggttagc cccccatgag gtagtcgtga cgctcgtcct gctggtgccg
      121 cttctgctgc tcgcagccgc ctgcatcgtg gtaatcggcc ccggcggctc ggagcctgcg
      181 cgggcgacgt tggccgccta tcccatcggc gccgccttcc ttggcgccgt tgcaaccctc
      241 tatctcgtga cgaccggcgg acccgtgtcg gtccgcttct acaatctgtc ctcggtggcc
      301 tcgttcgcga tccccatcgg cttctacgtc gaccggctga gcgcggtgat gatgacgctg
      361 atcaccggtg tgagcgtcat tatctacagc tactcgacgg gatacatgta ccaggaccgg
      421 cacgcccgtc ggtacctcgc catgatttgc ctgacggatt ttgtcctgat ctgcatggtg
      481 tcgagcgcca atctcatgat gctgtttctc ttttggcagg tgctcagcta tctgctctac
      541 gtacttgcgc acaatcatgc ccatgcggga acgctggcgg gagcgttcaa gacctttgcg
      601 cttctgcgcg tcgccgatac agcctttctc gccggcatcg cactcgcgta tcacctgtac
      661 ggcacgctcg aattccagga gctgtttcag cgagccgccg acatgccggt catgttgtcc
      721 ctctgggcgg gcatggacat gagcggcact acggccgtca cgctgctgct cttcgtcgga
      781 gcgatgggaa agtcggccca gtttccgatt catctctggc tccccggatc gctcttcgct
      841 ccgacccccg tgcacgcgtt gcttcacgcg ggcatcatca acgccggcgg gttcctgatc
      901 aaccggcttg cgccgttgtt cggcctcagc tcaattacgc tgcacgtcgc cttcctcatc
      961 ggaaccctga ccgccgtcct gggcgcctcc atgatgctgg cccaaaacga catcaaaaaa
     1021 acgctcgggt tctccacgat aggccaaatg gggtacatga tcatggaatg cgggctggga
     1081 gccttcgccc tcgcggtgtt ccatttgatc gcgcacggct tgttcaaggc aacggtcttt
     1141 ctgaactgtg gcaacgtcat tcataaggcc cgacaggaac cacactttcc ctacgtggac
     1201 caccacgctg aagaaccggg cttgtccggt ctgacgtggt tcacgggatc cgtgaccacg
     1261 ttgttcattc ccctgctcat tctcctggtc acgcatgggg tcttgcgcat cccgttgctg
     1321 gaatcgcatg ggacggtgat ttttctgttc ttcatctgga tcacctcgtc tcaagcgatt
     1381 ctcacgctga cgcgtctgcg ccaggtcgcg tcgtggaaag tctcggccgc catgttgatg
     1441 acgctgctct tcatcgtgtt cgtctatctg ttcgcggtcg agtcgttcac ggagtttctg
     1501 tatccgaatc cgggggaagt ggcctcgtac ttcaaggccg ccgatcttcc cgcctggctg
     1561 ttcgacctta tcattgtgac ggccacgatc ctgaccgtcg ccggttggtc ctacctctac
     1621 atgagcgcac atgggatgac gctacggatg ccggcttgga tcgacagtat gcggatacgg
     1681 ctctacctgc ccttcatgaa ccggctctat gccgatgaac tctacgcgct gacagggcag
     1741 accatcatgc ggctagtcca tcaactcgac aaacgagagc gaggctggtc acgatgatgg
     1801 acggcacgct gatcccatgg ctcttggctg gcatccctct gctgggggtg ctggcctccc
     1861 tgcccttccg atcggatccc gagcggctga aagggtcctc agtcgcatgg gccgtgatca
     1921 gcctgggctc ggtcgtggga ttcgccggcc ggatcgacgt tccactcgaa gggcttctgc
     1981 cgctgtacct cctgccgctg gccgcgacga tttcgatctt ggggcagccg gcgcacaaac
     2041 accaccggcg ttcatggatg ttaaccttgc tgtttttggg gttggggctc tccgccttga
     2101 gcagcgaacg gccgatcagt cttctgagcg tgtctctgct tttggtgctg gtcgcggtac
     2161 tgctctaccg ccaccacagc gcactctggc cccgatcatg gtggggcatc ggcgcgtacg
     2221 gtctcggcgc cttgtgcgcc gctctgtcgg tcgccgccga tgctccgctt tcctcggttg
     2281 cgtccttgct ggcctgtgcg gtcttgcttc cccttgtgcc gttccacgac ggtcacctga
     2341 cggcgctgac gcggttgccg ggcagcctgc cctcgttcat cgtcgtgttg ctcccgatgg
     2401 ccggtctcca tcaacttgcc tccgcgatgc ccaccgtacc ggacatcgtg gcctggaccg
     2461 tgagcctctt cgccctcgtg ggagcctcct atggcgcgct caaagcgttg gcgcaatccc
     2521 gaatccgtct cctgctggcc tacggcagcc tctcgttttt ttccattctc tggtggttca
     2581 ccgcagcctt ccgaatggtg acgccacggt ccgctcttct ggtcggaacg gtcggcctgg
     2641 cgacgagcgg gctcatggtc gcctggcaag tggtccgcac ccggtacggc gacgacgtgg
     2701 atccgcaagc catcagcggc cttgcgagtg gaatgccgca atatgcggtg ctgctgtcat
     2761 tgctggcgct ggccgcgatg ggcctgccgc ccttcggtgt atttgccgga tttatggggc
     2821 tgctgctgag ttcgccgttt ccctccctgc ccggcctgtt cgtcacgctg ctggcgtggc
     2881 ttgccgcgtc ctggtacatc atgcagatgg ttcaacggct gctcttcggc gtccggcggc
     2941 ccgaattgcg gtacgccgat ctgctccgcg ccgagttggc ttcgttgctg atcgtcgtcc
     3001 tggtccttct ggcgttggga ctggcgccaa cgaccctctt cgccccggag ggagccttgt
     3061 tcgggacctc caccatggga gaatcgccca cgtggactcg ctagatcgcg cgaccgacat
     3121 cgaagcgagg cggatggagc tgcgcggggt ggttcgcctc gccggagaag tcatcgcgca
     3181 atattggccg atgcggacgt tcgtgcatca caatccgctg cacagtctcg aataccttcc
     3241 gttcgaggaa acggtccgcc gcgggaagca atttatgggc ggcagcggct atcttcccag
     3301 cacgttgtat catcactacc tccgatcggg gcggatccgc cccgaacatc tggatcaggt
     3361 cttatgccac gtggcggatg acaaacgtgt cgtcatcggc gcccgcaccg tgacgcacgg
     3421 cgatgtgctg cgcgcctgtc tgacccagga attggtattg ccggttgcgg aaccgctcga
     3481 tagtcagctg ccggatcctt cgcatactct catcgagcag atcgcggccc gccttgagtc
     3541 ggtgctgacc tatcccgacc tatcaacacg cattcgcgcc atcgtggagg gcgaccaagc
     3601 cgccttggga caatggatca ccctttcgca ctggtgtgac gatacgctgg gtacgcagat
     3661 tgttcgccag atcaacgatc aactcatcaa atggtgtgaa gcgttccttg acgaggggca
     3721 cgcgacctgg gccatgccca aacgcgagaa aggtctgtat caggcctgga aaatgatcgc
     3781 cgctcatgaa tggtcgccct gcggcattgc cgacagcgga cgaaaaatcg ccgccctccc
     3841 cgatcatccg gaagacgctc tcctcgagag tctggatgcg ttgggcatcc cggccgagct
     3901 aagacaagac tatttatcgc tccagttgac cgcattgccg ggttgggccg gttttatcaa
     3961 gtggcgcggc gaggaacggg actatcggtg gcaacaagcg tatccggccg ggctcgtgaa
     4021 gttcttggcc atccgcctct ggtacgcgcg cgagttggtg cagaaggcct gccgcgaaga
     4081 actgggcata gaaggtcgct acgcggccgt gaccgcgtac atgcgcagcc gtccagaaga
     4141 gtactacttg cgacgacagc gggtggcggg gcgattgcca gccctgtacg ccgaggaagt
     4201 tgatctgctg gcccatcaga gaggcaacgg gtggaaggag gtgctccatc gctatcagat
     4261 cgaagtggtt ccacgacagg cggcagcagc gcggcgcggg gcggcaagaa cgctgctggc
     4321 cttggcgcaa tcgcttgcta tcgatccgga gcagctcgtc ggaactgctc ctgaaacgct
     4381 gaaacagatc gtccattggg tcgaggcctt tccagagtcg gcgcatggtc cggtctggct
     4441 caaggccctc gaagccagct atcaggaggc actgctaggg cagcttcgtc gcccgacagc
     4501 ggtccccgac ggaacggaca agtcacggcc ctattcgcag tctgtctact gcatcgacgt
     4561 gcgatcagaa cctttccgcc gacacttgga atcgaccggc tcgcatgaaa cgtacggatt
     4621 tgccggcttc ttcgccgcct tcatccgtta ccgagcctgg ggaaaggaac atgacactga
     4681 gcagtttccg gtgatcatgc gtgccaagaa cgaggtgcgg gaaatccccc ggagttatct
     4741 cgatcacaag gtatcgaagc acgaggctcg ggccagatgg gtacacgcgg gccacacgtt
     4801 gttacacgat ctcaaagaga acgtcgtgac gccctacgtc atggttgaat cgcttgggtg
     4861 gttctacggc attcccattt tcggaaagac actcttgcct tcgctgtacc ggcgctggac
     4921 cgcttggctg caacggctgt tcgttcccgc catcgccacg acgctcaccg tcgacaaact
     4981 ggcgccggcc gacacggccg agatggtcgc cgccgagcag caggccgtcg tccggaaggc
     5041 cctgcaggaa cggctcgggc tgcggagttc acggatgacg cccgcgctgg tcgaaagcct
     5101 gcgccagcac gcgttgtccg gatctgaaga gccggatcag ccgctgaccg acgccgccgc
     5161 cctggccggg ctttccaagg acgcgctggt cggtctcacc gaggtcttgc gccggcagta
     5221 cgaacttaac gctcgtgccg cctcccgcca caaagagcga atcacgcgaa cgggcttcac
     5281 gctcgaagag caaacgctca cggtcgacac ggccctccgc atgatggggc tgaccaaaaa
     5341 ttttgcgcgg ttggtcttgt tctgcgccca cggaagcacc tccgataaca atccgtacga
     5401 gtccgcgctc gattgcggcg cttgcggcgg caacgaaggc aagcccaatg cccgtgtgct
     5461 tgccatgatg gcaaacaacc agcaagtgag gggacgactc gccaagaacg ggatcgagat
     5521 cccgtccgat actcatttca tcgccggcca gatggacaca acgactgatg ccgtgcagct
     5581 gttcgacttg gaggacgttc ccccgacgca tcgagccgac ctggcccgtc tgcaggaaga
     5641 cctcgcgcaa gcgggggaac tgaccagcca ggaacgatgc gctcggctgc cggatgttca
     5701 acagccgatt gccggtaata aggcaaaagc gcacgtccgc acgcgcagca ctgattggag
     5761 ccaggtgagg ccggaatggg gcctgtcggg aaatgccgcc ttcataatcg cccgacgtga
     5821 gctgacgaag ggaatggatt taggcgggcg ggtcttcctc cattcctacg actaccggga
     5881 ggatccgacg aaccggctat tggaagtgct gctgaccgcc cctcaggtcg tcgcgcagtg
     5941 gatcaacatg gagcactatt tttccaccac ggataatgaa gtgtatggaa gcggcagcaa
     6001 ggtctatcac aatgtcgtag ggcggttggg catcatgtcc ggcccctgga gcgatctccg
     6061 tctgggtttg gccaggcaga cggtgatgaa cggggaggtt ccctatcacg aaccgatgcg
     6121 actgctcact atcatcgagg ccccccgcga gcggatcgac aagctcattg gccggcatga
     6181 ggtcttacaa cattactacc acaatcaatg ggtgcacctc gtcgccttcg atcctgacga
     6241 cggggtatgg tatcgatatc attcaaaggg aatgtggaac gtgattccgt aatctctgtc
     6301 gatgcaagac gtgcgcaata gcttgaaagc gtggaaagga gtgtgtgaat gggtctgaca
     6361 ttacatccta tgaaagaaat tcgcgtgatc gtagcgggag aacaccggtc gttcgtgact
     6421 gacttacttg accgggtcaa tgctaccggc tacaccatca tcgggaacgt gtccggcaaa
     6481 ggtcaccacg gcgtgcgcga ggcccatttc atgttcagcg agcaggaaag ccttgagatg
     6541 atcatgaccg tcgtgccgga agaaaaggtc gagccgatcc tggccggcct gcggccgctc
     6601 ttcgaacggc actccggtgt catgttcgtc ggcgacgtgg ctgtgagccg ggaggaatac
     6661 tttggcaaga agggcaaagg agcctgacca gccatcgtcg gtgatgcaac gccccgccct
     6721 tgtcttcacg cccagaactt gtctagaatg ggcccctctg aggcggttcc acgacggagg
     6781 gatcggtgaa gggtttacgg ttcgagcgta tcggccagga ccgctactat aatgtcgtct
     6841 tccacatggg cagcacctac gtgccggtga gcgacgaaac cgtagaggaa ttaaaggctc
     6901 agagtctgct gccgcccgag cgattcctcg atctgctcat cgatcggatc gggtattcgt
     6961 cctacctcaa ggatcagatt cgcaccgaac tgaagtcttc gggcgatcca gtcactcaga
     7021 tcaccgttct gcagggagcg atc
//
LOCUS       DATDNW010000251         5765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1194214, whole genome shotgun sequence.
ACCESSION   DATDNW010000251 DATDNW010000000
VERSION     DATDNW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194214"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VI359_06135"
     CDS             complement(<1..1199)
                     /locus_tag="VI359_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973865.1"
                     /translation="MTQPSHLLDLAGSNLRRCSHIRDKLVEAGLYALGPTENSSAPPL
                     PHPVTNSWRIAPLPFSLSQEQLTWFSALGSPLLSFYRALNRLYMESVRSTQPPWIASY
                     LDQGKPEALVTYSRMKRFRDQLPAVIRPDVIPTSDGMVITELDSVPGGIGLTACLSHL
                     YAETDGKHSSLVGGPDGMVQGFAAMLRSQLGSHTGCVAILVSEEAKDYRPEMRWLAEQ
                     LRDRNLTCYCVEPREVRFTEEGLFLSTGTEEKPIGLIYRFYELFDLMNIPKAELVQYA
                     VKKERVAVTPPYKPALEEKLAFALLHHPILNSYWQQELGHEAFLQLTTVMPRTWILDP
                     APVPPSATIPGLSLGGRAVANWTDLAAATQKERHFVIKPSGFSELAWGSRGVSVGHDM
                     PQTEWAAA"
     gene            complement(1243..2250)
                     /locus_tag="VI359_06140"
     CDS             complement(1243..2250)
                     /locus_tag="VI359_06140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY6973866.1"
                     /translation="MATTPSSDQPQYDARNLLNSQPVIITVIDPNTHKVVFQNHTSQI
                     KFGDISNQTCHEKIAGCPAPCQFCKMPEAVETGKPTASEVPLPNDEHLLVQWAKVDTT
                     DGSTHIVETITDITSQKRQQAQTELLVKNLSETNRDLLHVNQQLRDQSTRDSLTGLYN
                     HSHFQQTLVQWCAQALRSMRPISLLFIDLDDFKYINDTYGHAGGDQVLREIGWLLDSQ
                     QGPGHGIARSSDFAARYGGDEFAMILPDTSMDGALSVAERLQHRMTTLTLLPELAKLA
                     TPPLSLTCSIGIATFPIHASVPSDLIVAADIAVYAAKNAGKNCVRMATPAAPPTPLVR
                     S"
     gene            2418..3920
                     /locus_tag="VI359_06145"
     CDS             2418..3920
                     /locus_tag="VI359_06145"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEY6973867.1"
                     /translation="MDALAIQTAHAAVKSHAELPQIVPSSTCLRCDVCCRFPDPDSAL
                     RPYFTAEDIELAVAAGVGRSAFSNSHGSQVTLVPDQRNEGYQCPAFDPATSHCTIYDR
                     RPFDCRIYPLALMWNEAHDQVVLGWDTKCPFLRDQVPADIHRHAVQVMELVNQPNVVK
                     MLGAHPRLVGRFQEDVVVLASLPHVTAAVSSRWVGARLYQLTVGDIPRLTSALEQSLT
                     MESVAPSLAAYSVPYHYMWNGLLAYWWAELHGALCLFIQSPDGWFMPLPPLGTSSIEQ
                     PLDEAFALMRRWNGASPVSRVENVPEQLAPDLERRGYRLAPKESDYVYGAIDLGTLAG
                     DRYKSQRALCNRVEREAAIIVEPYRIGDRSGCRALFRDWSKQKHAEGIGEFGRMLLAD
                     AEPAHEVLWSHAPALSLTGTVVRIEGRIWAYTFGYWLNNTMFCVLAEVADRTIPGLAQ
                     FLFRETCRSASAQGAEFINTMDDAGLIGLRASKQAYHPIAKVPNFIASRA"
     gene            3997..>5765
                     /locus_tag="VI359_06150"
     CDS             3997..>5765
                     /locus_tag="VI359_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6973868.1"
                     /translation="MTVLTLAAGLFLVRYVERQLVDATGEQLTLAAAEVSDKLDRLLF
                     ERYGDVHMMARAFSLRASDPQYLSEYLAWMKAKYDPVYFWLGVTDSQGTIVATTNQAL
                     LGQDKSRMDWFQQVRSTRRALVQDVAIHEADNGVETVAFTVPILDSQKTFLGVVTARI
                     TVPTLEEVTTRTIRSLEDRAGFVGSVEYQMLTRRGDVFVDSDLLHKAPINLKALGLPS
                     ALAAQSGIPGFLEERHLRRGVSVVSGYAQTHGHGDFVGFGWSVLVRMDRQAILGPIQS
                     ALWSVGLAAAIVWVPLLILLLRATSWLRDEYQKAQQERAWASAAEAALLQSQERNRAV
                     VDTALDGVITIDASGLITDWNAQAEKMFGWSRADVLGRRLSETIIPPRDREAHEQGLR
                     HFLGTGESHILNRRIEVLALHRDGHEFPVELAVSPARIGEAYFFSAFLRDITEQKEAQ
                     TKLRDSEARYRSVVNALDEGVVLVDTQGVIRTGNISAERILGLSLDEMVGRSLRDARW
                     QTVYEDGSPFLPDEYPASVTLRTGQPCSGVIMGVYKPDGVRRWIAVNSRALMGSDHRT
                     AAVVVSFTDITERKRTEDRLAAQ"
BASE COUNT         1155 a         1537 c         1792 g         1281 t
ORIGIN      
        1 gcggccgccc attcggtctg cggcatatcg tgcccgaccg agacgcctct gctcccccag
       61 gccaactccg aaaatcccga tggtttgatg acgaaatggc gttccttttg tgtcgccgcg
      121 gcgaggtctg tccagtttgc aaccgcccgc ccgccgaggg agagacctgg gatagttgcc
      181 gacggaggaa cgggggcagg atccaatatc caggttcggg gcatgacagt cgtcaattgg
      241 agaaatgcct cgtgacccaa ttcctgttgc caataagaat tcaaaattgg gtgatggagg
      301 agggcgaagg cgagtttctc ttccagtgcc ggtttatatg gtggagtcac ggcaacccgc
      361 tcttttttga ccgcatactg caccagctct gcttttggga tgttcatcag atcgaacaac
      421 tcgtagaatc gatagatgag accgattggc ttttcttcgg tgccggtgga gagaaaaaga
      481 ccctcttccg tgaatcggac ctcacggggt tctacacaat aacaggtcaa gtttcggtcg
      541 cggagttgct cagccagcca tctcatctcc ggccgatagt ccttggcttc ttccgagacg
      601 agaatggcca cacagccagt gtgcgaaccc agttggctgc gcagcattgc ggcaaagcct
      661 tgaaccatgc catcgggtcc gccgaccaga gaggagtgtt taccgtccgt ctcggcatac
      721 agatgagaca gacaggcagt cagtccgatg ccacctggga ccgaatcaag ctcggtgatg
      781 accatgccgt ccgaggtcgg gatcacatcc ggtcgaatca ctgcgggaag ttggtcgcga
      841 aaccgtttca tgcggctgta ggtcacgagc gcttcaggct taccttggtc cagatagctg
      901 gcgatccatg gcggctgagt gctacggacg ctctccatgt agagacgatt caaggcccgg
      961 taaaacgaca gaagaggcga gccgagggcc gagaaccacg tgagctgctc ctgtgagaga
     1021 gaaaagggaa gaggagcaat gcgccacgag ttggttacgg gatgaggtag tggcggggca
     1081 gaagaattct ctgttggccc cagggcatac aacccggcct cgaccagttt gtcgcggatg
     1141 tgtgaacagc ggcggagatt agacccggct aggtcaagca gatgcgatgg ctgtgtcaat
     1201 gatcatgccc tcatgcgagg cgaaatgatc gtaacatggg tcttaggatc gtacaagagg
     1261 tgtcggtggg gctgcgggtg tcgccatgcg gacacagttc ttcccggcat tcttggcggc
     1321 atacactgcg atatccgcgg ccacgatcag gtccgagggg acggatgcgt ggatcggaaa
     1381 cgtggcgatc ccgatgctgc aggtcaatga taacggagga gtcgccagct tggccagctc
     1441 cggcagcaac gtcaatgtcg tcatccgatg ctggagtcgt tcggccacgc tgagtgctcc
     1501 gtccatggat gtgtccggca ggatcatcgc gaattcgtcc cctccgtagc gcgcagcgaa
     1561 atcgctggac cgggcgatgc catggcccgg tccttgttga ctgtcaagga gccaaccgat
     1621 ctcgcgtaac acctgatcgc cgcccgcgtg accataggtg tcgttgatgt atttgaaatc
     1681 gtcgagatcg atgaagagca gcgagatcgg gcgcattgac cgaagggctt gggcgcacca
     1741 ctggaccagg gtctgttgaa aatgtgagtg attatacagg ccggtcaggc tgtctcgcgt
     1801 cgattggtcg cgcagttgct gattgacgtg gagcaaatcg cggttggtct ctgacaggtt
     1861 cttgaccaga agctcggtct gagcctgttg tcgcttctgc gatgtgatat cggtaatcgt
     1921 ttcgacgatg tgagtgcttc cgtcggtcgt gtcgactttg gcccactgaa cgaggagatg
     1981 ttcgtcgttt gggagcggta cttcacttgc cgtcggtttg ccggtttcga cggcttccgg
     2041 cattttgcaa aactgacagg gcgcgggaca accggcaatt ttttcgtgac aggtttgatt
     2101 cgagatgtct ccgaatttga tttgcgacgt atggttctga aagacgacct tatgggtgtt
     2161 cggatcgatg acggtgataa tcaccggctg tgagttcagc aggttcctcg cgtcatactg
     2221 aggctgatct gatgatggag tcgtagccac tgttgagcct ctgtacaggt cctgccatga
     2281 gagtattcca ccaggggtaa gaccggtcaa gaaattgcgc agtttcactg tgaaagcacg
     2341 actcagaaaa tcgcgacctg ttaggtggta tccgtaattg gtgttagaat gaccgcgaaa
     2401 ttgataggaa tccgtgcatg gatgcgcttg caatccagac agcccacgct gccgtgaaat
     2461 cgcacgccga acttccccaa atcgtgccca gcagcacctg ccttcgctgc gatgtttgtt
     2521 gccggtttcc ggacccggac agcgccctgc gtccgtattt caccgcggag gatatcgagc
     2581 tggcggtagc ggccggcgtc ggtcggtcgg cgttttctaa ctctcatggc tcccaagtga
     2641 ctctggttcc cgatcaaaga aacgagggat atcagtgtcc agcgttcgat ccggcaacct
     2701 cgcactgtac gatctatgac cggcgtccct tcgattgcag gatctatccg ttggccctga
     2761 tgtggaacga ggcgcatgac caggtcgtct tgggctggga taccaaatgc ccgttcctgc
     2821 gcgaccaggt tccggcagat attcaccgtc atgctgttca ggtgatggag ctcgtgaatc
     2881 aaccgaacgt cgtgaagatg ctgggggcgc atcctcgtct cgtcgggcga tttcaagagg
     2941 acgttgtcgt gctggcttct ctgccacacg tgactgccgc ggtctcaagt cggtgggtcg
     3001 gcgcccggtt gtatcagctc accgtcggcg atatcccgcg cctgacttcg gcgctggagc
     3061 agtcactcac gatggagagc gttgcccctt cgcttgctgc gtattcggtg ccctaccatt
     3121 acatgtggaa cgggctcttg gcctattggt gggcggaatt gcacggagcc ttgtgtctct
     3181 tcatccaatc gccggatggc tggttcatgc cgcttccccc tctcggaacc agctccatcg
     3241 agcagcctct ggacgaggca ttcgcattga tgcggcggtg gaacggtgcg tcgccggtca
     3301 gccgggtcga aaacgtgccc gagcaactcg caccggatct ggagcgacgc gggtatcgac
     3361 tggcgccgaa agaatcagac tatgtgtatg gtgcgatcga tctcgggacc ctggcgggtg
     3421 atcgatacaa gtctcaacgg gctctctgca atcgtgtcga acgagaggct gccatcattg
     3481 tggagcccta tcggattggt gatcgttctg gctgtcgtgc gctctttcgg gattggtcga
     3541 agcagaaaca tgctgagggg atcggggagt ttggccgcat gcttcttgcc gacgcggagc
     3601 ccgctcacga agtgctctgg tcgcatgcgc cggccctcag tctgacggga acggttgtcc
     3661 ggatcgaagg gcggatttgg gcctatacgt tcggttactg gttgaacaac acgatgttct
     3721 gcgtgttagc ggaagtggcg gatcgcacaa ttccgggtct cgcgcaattt ctgtttcggg
     3781 aaacgtgccg gtctgcatcg gctcagggcg cagaattcat caacacgatg gatgatgcag
     3841 gtctgattgg actgcgcgcg tccaaacagg cctatcaccc catcgcaaag gttccgaatt
     3901 tcatcgccag tcgagcatga catgatcgcc ccatccaata ctccgaggtc cgtacattct
     3961 cgccgttacc ggtggttgcc gtacgtgatc atcgtcatga cggttctgac attggcggcc
     4021 ggtttattct tggtgcgata cgtcgaacgt cagttagtgg acgcgaccgg agaacaactg
     4081 acactcgcgg cggcggaggt gtcagataag ctcgaccggc tcttgttcga acggtatggc
     4141 gacgtccaca tgatggcgcg agcgttttcc ttgcgcgcct ccgatccgca atacctttcg
     4201 gaatatctcg cgtggatgaa agccaaatac gatcctgtgt atttctggct cggggtcacc
     4261 gattcgcaag ggactattgt ggcaacgacg aatcaagcct tgctcggaca agacaaaagc
     4321 cgcatggatt ggttccagca ggttcgctcg acacgacgag cgttggttca ggatgtggcc
     4381 atccatgagg ccgacaacgg ggtggaaacg gtcgccttca ccgtgcccat tctggattct
     4441 caaaagacgt tcctcggagt cgtgacagca cggattaccg tgcccacact ggaggaagtg
     4501 acaacgagga cgattcgatc gctcgaagat cgcgccggat ttgtcggatc agtcgaatat
     4561 caaatgctga cccggcgtgg cgatgtgttc gtcgactcgg acttgcttca taaggcaccc
     4621 atcaatctca aggcgctcgg cctcccgtcc gctctcgcgg cacagtcggg tattccgggg
     4681 tttctggaag aacggcatct gcgccgaggc gtgagcgtcg tcagcggata cgctcagacc
     4741 catggtcatg gagacttcgt cggattcggg tggtcggtgc tcgtacggat ggatcgccag
     4801 gccattttgg gtccgatcca atccgccttg tggagtgttg ggctcgccgc cgccattgtt
     4861 tgggtgcctc tcctcatcct gctgctccgg gccacgtcgt ggttgcggga cgagtatcaa
     4921 aaagcacaac aagaaagggc ctgggcgagc gcggcagaag cggcgttgct gcagagccag
     4981 gaacggaatc gcgccgtggt cgataccgct ctggacggcg tgatcaccat tgatgcgagc
     5041 gggctcatta cggattggaa tgcgcaggca gaaaagatgt tcgggtggtc tcgagccgac
     5101 gtgcttggtc gacgcttgtc ggagaccatc atcccaccgc gagatcgaga ggcgcacgag
     5161 caagggctac gccatttcct agggacgggg gagagtcata tcctcaatcg gcgcatcgag
     5221 gtcctggcgc tgcatcgcga cgggcatgaa tttcctgtgg aactcgctgt ctcgcccgcc
     5281 cgcatcggcg aggcctactt tttcagtgcc tttctgcgtg acatcaccga acagaaagag
     5341 gcgcagacca agttgagaga cagcgaggcg cggtatcgtt ccgtcgtcaa cgcgttggac
     5401 gaaggtgtcg tgttggttga tacgcaaggg gtcatacgaa cgggaaacat cagtgccgag
     5461 cggatcctgg gattgtctct ggatgaaatg gtgggacggt cgctccgcga tgccagatgg
     5521 cagaccgtgt acgaggatgg atcgcccttc cttcctgatg aatatccagc ctcggtcacc
     5581 ttgcgaacag gacagccctg ctccggagtg atcatgggtg tctataaacc ggatggggtg
     5641 cgtcgttgga ttgcggtgaa ttcccgagcg ctgatgggct cggatcatcg gacggcagct
     5701 gtggtcgtgt cgtttacgga tattaccgag cgcaaacgga cggaggaccg actggcagct
     5761 cagta
//
LOCUS       DATDNW010000252         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_169550, whole genome shotgun sequence.
ACCESSION   DATDNW010000252 DATDNW010000000
VERSION     DATDNW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169550"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2361
                     /locus_tag="VI359_06155"
     CDS             <1..2361
                     /locus_tag="VI359_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY6973869.1"
                     /translation="PLPMMMPTASPQSVGEIDPVCGMTVQPATAAGSYKHQGTTYYFC
                     SKSCLEKFRSNPQSYLTPPDQRAPHIASASSGGAVEYICPMDPEVVESKPGACRICGM
                     ALEPKMIVLDGARNPELDDMTRRFWLCLGPAIIMMIIAMSDMVPIPPVLQSVSRLTLN
                     WVQWMLATPVVLWGGWPFFQRGWTSIVNIAPNMFTLIAIGTGTAYLYSTVATIAPSLF
                     PASVRQPDGSVAVYFEAAAIITLLVLLGQVLELRARSQTTSALRSLLQLAPNTARRLL
                     LDGQEIEVPLDHVQVGDRLRVRPGERIPVDGSVVEGITVVDESMMTGESIPVEKTIGS
                     RVIGGTINGTGTVLMQAERVGRETLLARIVQMVSEAQRTRAPIQRVADMAAGYFVPAV
                     VGVALVTAVVWALWGPEPRLAHAVVNAVAVLIIACPCALGLATPMSIMVGTGRGATAG
                     VLVRHAEALEVFGRVDTLVVDKTGTLTEGKPTLVAVRFVPPWTDVDLLRLAASLERTS
                     EHPLAAAVVEGAASRGIGLTTGQQFQSVTGRGVKGIVEGKQLAIGTVAFLQEDAGVSS
                     QSMAALNDEAEPLRREGQTILFVAVDGQAAGLLGVADRIKPSTPDAIAYFKDEGVRLV
                     MVTGDHRETAEAVARRLGIGDVMAGVLPDQKGQIVQQLKSQGRVVAMAGDGINDAPAL
                     ALADVGIAMGTGTDTAIESAGMTLVKGDLRALVRARRLSQSTMRNIRQNLFFAFLYNV
                     LGVPIAAGVLYPVLGILLSPMIASAAMTLSSVSVISNALRLRHVEL"
BASE COUNT          495 a          715 c          840 g          546 t
ORIGIN      
        1 ccgcttccta tgatgatgcc gaccgcttcg cctcagagcg ttggcgagat cgatccggtg
       61 tgcggcatga cggtgcagcc tgcgacggct gccgggtcgt ataagcatca gggcacgacc
      121 tactatttct gttcgaagag ctgccttgaa aagtttcgat cgaatcctca gtcttacctg
      181 actccgccgg accagcgggc gcctcatatc gcctcggctt cgtccggtgg agctgtcgag
      241 tatatctgcc cgatggatcc tgaggttgtg gagtcaaagc ccggagcctg tcggatatgt
      301 gggatggcgc tggagccgaa aatgattgtg ctcgacggtg cgcgcaatcc cgaactcgac
      361 gatatgacca gacggttctg gctctgtctc ggccccgcga tcatcatgat gatcattgcg
      421 atgtccgaca tggtcccgat cccgccggtt ctgcagtcag tttcccggtt gacgttgaat
      481 tgggttcagt ggatgttggc cactccagtc gtgctctggg gaggatggcc gttcttccag
      541 cgggggtgga cgtcgatcgt aaatattgcg cccaatatgt tcacgctcat cgcgatcggg
      601 acagggacgg cctatctcta cagcaccgtc gcgacgatcg ctccgtccct gttcccggct
      661 tccgtccgac agcccgatgg ctctgtggcg gtgtattttg aagcggcggc aattattact
      721 ctgttggttc tcttagggca ggtcctggaa ctgcgtgcgc gcagccaaac gacctcggca
      781 cttcgatcgc tcttgcagct ggcgcccaat acggcgaggc gcctcttgct ggatggtcaa
      841 gagatcgagg tgccgctcga ccacgtccaa gttggagatc gccttcgcgt gcgtccgggc
      901 gagcgcatcc cggtcgacgg aagtgtggtc gaagggataa cggtcgttga tgagtcgatg
      961 atgacaggtg aatcgattcc cgtggagaaa acaatcgggt ctcgagtgat cggcgggacc
     1021 atcaatggga cgggtacggt gctgatgcag gcggagcgcg tcggacgaga gacgttgctg
     1081 gcgaggattg tgcaaatggt gagcgaggcg cagcgaaccc gggccccgat tcagcgagtg
     1141 gccgatatgg cagccgggta ttttgtgccg gccgttgtgg gagtggcact ggtcacggcc
     1201 gtcgtgtggg ccttgtgggg tccggagcca agactcgccc atgcagtcgt gaatgccgtc
     1261 gcggtgctca tcatcgcctg tccttgtgcc ttgggtttag caacaccgat gtcgatcatg
     1321 gtgggaacag gacgcggagc gactgccggc gtgctggtca gacacgcaga ggcgttggaa
     1381 gtcttcggga gggtcgatac gttggttgtg gacaaaaccg gcacactgac cgaaggaaag
     1441 cccacactcg tcgcagtccg cttcgtgccg ccttggaccg atgttgacct gttgcgcctg
     1501 gccgccagct tggaacgcac cagtgaacat ccgctcgccg cagcagtggt ggagggagcc
     1561 gcctcgcggg ggatcgggct gacgacgggc cagcagtttc aatccgtgac cggcagaggt
     1621 gtgaagggaa tcgtcgaggg taaacagctt gcgatcggaa ccgtcgcatt cttacaggag
     1681 gacgccgggg tctccagcca gagcatggct gcgttaaacg atgaggctga accactgcgg
     1741 cgtgagggac aaaccatctt gtttgtagct gtggatgggc aagcagcggg gctgctcggc
     1801 gtcgcagacc gcatcaaacc ctcgacaccg gacgcaattg catacttcaa ggatgaaggc
     1861 gttcgcctcg tcatggtgac aggcgaccat cgcgagaccg cagaagcagt ggcgcgacgg
     1921 cttggcatcg gcgacgtcat ggctggggta ttgccggacc aaaaaggaca gattgttcag
     1981 cagctcaagt cgcaaggccg cgtcgtggcg atggcgggtg atggcatcaa tgacgcacca
     2041 gccctggcgt tggctgatgt cgggattgca atgggaactg gaacagacac cgcaatagaa
     2101 agtgcaggga tgacattggt gaaaggggat ttgcgggcac tggtgcgggc acgccggctc
     2161 agccaatcca caatgcggaa cattcgtcaa aatctgttct ttgcgttcct ctacaacgtc
     2221 ctcggggtgc caatcgctgc aggagtgctc tatccggtac tgggaatact gctcagcccc
     2281 atgatcgcca gcgcggccat gacgttgagt tcggtctccg tcatctccaa tgccctccgc
     2341 ctacgccatg tggaattata agtgcacctc ttccccacgc atgctaggct acagcagccg
     2401 ttcagaggtc actgtcgtac cgtgagacgc tcacgatgaa gatgcgcgtt gtacacatcg
     2461 ctttcctccc gtgggtcatg gccgcatggt gtgtctggga tatttggatt ccggccgtcg
     2521 caccggctgg cactcggttg cttccaatat ccgcggcggg cgatcttcaa ggacaggtga
     2581 aatccactgc cgcgaa
//
LOCUS       DATDNW010000253         4234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_169554, whole genome shotgun sequence.
ACCESSION   DATDNW010000253 DATDNW010000000
VERSION     DATDNW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169554"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..835
                     /locus_tag="VI359_06160"
     CDS             266..835
                     /locus_tag="VI359_06160"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEY6973870.1"
                     /translation="MSACRTLPDYIRPDLTVLFVGINPGLRSAQVGHHFAGHSNRFWK
                     LLYESGLVTDQLTYQDDWRLPEWGLGLTNIISRSTAGIDVLRPHEYRAGVVRLRNKVA
                     RFRPKVIALLGVTIFRMIFPAQTPPSSRLSLGFQTARLTGVPVFLLPNPSGRNAHYSY
                     RRMLTTFQALRRAALQERVRRPRLPLHQR"
     gene            1248..1709
                     /locus_tag="VI359_06165"
     CDS             1248..1709
                     /locus_tag="VI359_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973871.1"
                     /translation="MAMESGRPHSLSLTEAELNHWMQDNLAIASARQAGEANLNLPNG
                     HEATIQHVHSALKDVRINMIDHQLLAYALFTIYGRDISLQLDGTLETTDGILRLKPLT
                     GKLGSLPIPRSMLDHVVHQLFESPENRDNFKLPPQIASVWVENSTLVVVTR"
     gene            1833..2612
                     /locus_tag="VI359_06170"
     CDS             1833..2612
                     /locus_tag="VI359_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY6973872.1"
                     /translation="MLLDGKKGLIIGVANKHSIAWAIAQSTASQGARLLFNYQNERLK
                     ENVEELVATMPGSKAFACDVGDDVQIEALMQHVHKEVGQLDFLVHSVAFAPREELTGQ
                     FVNTTRKGFATALDVSAYSLVAVTRAAMPLMTEGGSVVTLTYLGAERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAISAGPIKTLAARGVSGISKMVDHHKEFAPLRHAT
                     EQGEVGDTALFLISPLGRGITGEVIYVDGGYHILGSLTSAE"
     gene            complement(2621..3031)
                     /gene="arfB"
                     /locus_tag="VI359_06175"
     CDS             complement(2621..3031)
                     /gene="arfB"
                     /locus_tag="VI359_06175"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY6973873.1"
                     /translation="MLYVSQHVTIPDSEIEIHAVRSQGAGGQHVNKVSSAVHLRFDIG
                     ASSLPLFYKEALLTLRDHRISEDGVITIKAQSYRSQEQNRADALNRLQALVHSVATPR
                     KKRKATKPTKSSQNRRLESKVKRGRLKALRGRLE"
     gene            complement(3060..3962)
                     /locus_tag="VI359_06180"
     CDS             complement(3060..3962)
                     /locus_tag="VI359_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973874.1"
                     /translation="MLALTLGSFFAISGGTVAGQSDVAPTFRDQPHEVRDLRVGERYR
                     GGMRVRAPFVGISFVVPKDWRASLPAGSVVFLDSAVAAGLGTIHLLTDVTRDHLHVQL
                     SEPQSIEAGFVLHPVGSLQEEGRKLVGHYAGGDDVGVIIAVLGPSHNAAIYQFVGRKT
                     EVQHYRKLAEELASSTQFINEQDGPVLRAWYERLTGMVLTPQLDSSIVQSVGSTAIHL
                     CSDGRFIQIIRLQLVPGQPVDGESDGAYHETGTWRIEVQGAKAELVLTKLTGGADQHE
                     VLRHDDQYLLDGRVALARASDSCL"
     gene            complement(4042..>4234)
                     /locus_tag="VI359_06185"
     CDS             complement(4042..>4234)
                     /locus_tag="VI359_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973875.1"
                     /translation="SDEDLKHLTTLLLYHLVERCGGQVQFKVEDAQRVRASLSTKMVQ
                     MQVGNEVNLRIIDRLPELS"
BASE COUNT          914 a         1273 c         1100 g          947 t
ORIGIN      
        1 ccactgcgac cgccataatt gccatcaaca tgcgcttcat aaattcaccc cctttgagtt
       61 taagagattc gcgccgtttt cccggcactg gggttgatgg tcagcaagct taatgccgaa
      121 tcgaagcctc gtgacaataa cgctgtttat caatgtgtta tgcctctatt gatatgaggc
      181 attgaaggct tccccctgct gttttctgtg gcggaatggt tgacggcata ctaatctgcc
      241 tcagaccctg ctggagccct tggacatgag cgcatgccga acactccctg attacattcg
      301 cccagatctg acagtcctgt ttgtcgggat caatcctggg ttgcgatccg cccaggtggg
      361 tcatcacttc gcgggccatt ccaatcgatt ctggaagctc ctttatgaat ctggactggt
      421 caccgaccag ctcacctatc aggacgattg gcgcctacct gaatggggat tgggtctcac
      481 caatatcatt agccgcagca ctgcgggcat cgatgtcctc agaccgcatg aatatcgagc
      541 cggcgtcgtc cgattgcgaa acaaagtggc ccgcttccgg cccaaagtca ttgcgttgct
      601 cggcgtgacc atctttcgca tgatcttccc cgctcaaaca ccgccctcct cccgactgtc
      661 actaggtttt cagactgctc gcttgaccgg tgtaccggta ttcctactcc cgaatccaag
      721 cggccggaac gctcactatt cctatcgtcg catgctgacg acctttcaag cgctgcgccg
      781 cgcggctctg caggaacgag ttcgtcgacc ccgccttcct cttcatcaac gctgacagag
      841 acccctgatg atttttcgga atattttgcc tctccgacct cccttgccta tagtaggaac
      901 tcatgaaaat tgcacaatcg gaaccggcaa ccgagtccct tgttacttgc ccgaaatgcg
      961 gccgggaaca ggcaccggct gatgaatgca tgcggtgcgg gatcgtcttc gcgaaataca
     1021 aaccacaaga caaccttagt catgaaaccg ctctgccagc cagttggtcc agatctacgc
     1081 cgagtgcttc aggatttggt tggcgcctct ttcgccttct cccttggatc tcgttagcga
     1141 tcaccgccac catactgatt ctgattctta aacaggcgcc gccgctcgca atccaaaccg
     1201 atcctcaggc agctgaccga gtcactgaga agatagccca gctgcggatg gccatggaat
     1261 cgggtcgtcc tcattctctc tcgctgaccg aagcggaact caatcactgg atgcaggaca
     1321 atctggcgat cgcctcagcc cgccaagctg gagaagccaa tctgaatctg ccgaacggtc
     1381 acgaagccac catccagcac gtgcactcgg cgctgaaaga tgttcggatc aatatgatag
     1441 accatcagct cctggcctat gccttgttca ccatctatgg gagggacatc tcgctccaac
     1501 ttgatggcac gcttgaaacc acagacggca tccttcgatt aaaacccctc acgggcaaac
     1561 ttgggtcgtt gcctatcccg cgttccatgc tggatcacgt tgttcaccag ctcttcgagt
     1621 caccagagaa tcgtgacaat ttcaaactcc cgccgcaaat tgcctcggtc tgggtcgaaa
     1681 acagcactct cgtcgttgtc actcgataac gtcgtatcgc ttcggcaatg ctctgaactg
     1741 cgtccagttc tcgctcctgg aacaagcccg caaatcgtgc tatcttcagc gcccagctga
     1801 aaactatttc accatccttt caggagaacg cgatgttact agacgggaag aagggattaa
     1861 tcatcggcgt cgccaacaag cacagcatcg cctgggccat cgcccaatcg accgccagcc
     1921 agggtgccag gctactcttc aactaccaga acgagcgatt aaaggagaat gtcgaggagc
     1981 ttgtggccac gatgccgggc tcaaaggctt ttgcttgcga cgtgggggac gatgtgcaga
     2041 tcgaagccct gatgcagcat gtccataagg aggtcggcca actcgacttc ctggtccact
     2101 ccgtcgcctt cgctcctcga gaagaactca ccggccagtt cgtcaacaca acacgcaaag
     2161 ggttcgccac ggcgctcgac gtgagcgcct attcgctcgt ggcagtcacc cgcgcggcca
     2221 tgccgctgat gaccgaggga gggtcggtcg tcacgttgac gtatctgggg gccgagcgcg
     2281 tcgtgcccca ttacaacgtg atgggcgttg cgaaagccgc gctcgaagcg accgtgcgct
     2341 accttgcaca cgacctcgga ccgaagaaca tccgcgtgaa cgccatttcg gccggaccca
     2401 tcaaaactct cgcggctcgc ggagtctccg gcatcagcaa gatggtggat caccacaagg
     2461 agttcgcccc gcttcgtcac gcgacggagc aaggggaagt cggagacacc gcgctgttcc
     2521 tgatcagccc gctcgggcgt gggattaccg gtgaagtgat ctacgtggat gggggatatc
     2581 acattctcgg atcactgaca tcagcggaat agtgacgcag ctactcgagt cttcctctga
     2641 gggccttcaa tcggcctctc ttcaccttac tctctaaccg tctgttctgg gaactcttcg
     2701 ttggcttcgt ggccttcctc tttttgcgtg gggtggcgac gctgtgaacc agtgcctgga
     2761 gtcgattgag ggcatcggct cgattctgct cctggctccg atacgactgg gccttgatcg
     2821 taatcacacc gtcttctgaa atacggtgat ccctcagcgt cagcagcgct tccttgtaaa
     2881 acagcggcaa tgaggaggca ccaatatcga accgcaaatg gactgcgctc gagaccttgt
     2941 tgacatgctg gccaccggcc ccttgcgatc gcaccgcatg gatctcaatc tctgaatcgg
     3001 ggatggtgac atgttgcgac acgtacagca tagggagtct cgtaatgagt tgatctctgc
     3061 tacaggcaac tatcgctggc ccgagccaaa gccactcgtc cgtcgagtaa gtattgatcg
     3121 tcatggcgga gcacttcgtg ctggtccgcc cctcccgtta atttcgtcag aacgagctcg
     3181 gctttggcgc cctggacttc gatccgccag gttccggttt catggtaggc gccgtcgctc
     3241 tctccatcta ctggctggcc tggtaccaat tgcaaccgaa tgatctgaat aaaccgacca
     3301 tcgctacaga ggtggatggc cgtcgaccct acactttgca caatactgct gtccagttgc
     3361 ggagtcagca ccatccctgt gagccgttca taccacgccc tgaggacagg accgtcctgc
     3421 tcgtttatga actgggtcga tgatgccagc tcctccgcca gttttcgata atgttggact
     3481 tccgtcttgc gacccacaaa ttgatagatg gccgcgttat gggatggtcc taacacagcg
     3541 atgatgacac cgacatcgtc accccccgca taatgtccga cgagtttccg tccttcttct
     3601 tgaagtgatc caaccgggtg caagacgaag ccggcttcga ttgattgtgg ctcactcagt
     3661 tggacgtgca gatggtcgcg ggtgacatcg gtaagcagat gaatggtccc aaggcccgcc
     3721 gccaccgccg aatcaagaaa tacgactgag ccagccggaa gagatgcacg ccagtccttc
     3781 ggcacgacaa atgatatgcc gacgaatggt gcgcgaacgc gcatgcctcc gcgataccgt
     3841 tcgccgaccc gtaagtcccg aacctcatgc ggttgatctc ggaaagtcgg tgcgacgtcg
     3901 gattgccctg caaccgtccc tcccgagatg gcgaaaaaag agccgagtgt cagtgcgagc
     3961 atgcgtctca tgccggatat tggaccatgt ttcacaagga ggactcaacc gaatggcggc
     4021 agggcgaagg tagactggaa actacgacag ctctggaaga cgatcaataa tgcgcaagtt
     4081 cacttcatta cccacttgca tctgaaccat tttggtggac aagctggctc ggacccgctg
     4141 ggcgtcttcg accttgaact ggacctggcc cccacaacgt tcgaccaagt ggtacagcag
     4201 cagcgtggtc agatgtttca aatcttcgtc gctg
//
LOCUS       DATDNW010000254         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_99651,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000254 DATDNW010000000
VERSION     DATDNW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99651"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VI359_06190"
     CDS             complement(<1..494)
                     /locus_tag="VI359_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY6973876.1"
                     /translation="MADRAVGVPIVKEGIPFVGIGAGVTLLAGYLGWTVVAVVAGGLT
                     VFTAWFFRNPTRVIPQGPGLVVAPGDGTVIAIEEEFEPRFIKDRSVRLTIFLNVLDVH
                     VNRIPCEGVVEDIQYQPGAFLVASKPEATLKNEQNALFLRTAQGARVLCVQVAGLIAR
                     RIVC"
     gene            complement(509..1546)
                     /gene="ilvC"
                     /locus_tag="VI359_06195"
     CDS             complement(509..1546)
                     /gene="ilvC"
                     /locus_tag="VI359_06195"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY6973877.1"
                     /translation="MCLSKEWMMKIYYDKDADLQQIRSKKVAVIGYGSQGHAHALNMK
                     ESGVDVVIGLREGASWKKAEQSGLKVMPVADAVKASDVVMILAPDEAQATIYRQDIAP
                     HLKSGAYLAFGHGFNIHFGQIVPPATVNVFMVAPKGPGHLVRSEYTKGSGVPCLLAIH
                     QDPSGTTRQVGLAYASAIGGGRAGVIETNFREETETDLFGEQVVLCGGLTSLIQAGYE
                     TLVEAGYSPEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTEQ
                     TKQEMKTILDEIQSGRFAKEWVLENQANRPVYNALLAKGEAHPIEAVGAKLRAMMPWL
                     KKDQLVDKSKN"
     gene            complement(1589..2107)
                     /gene="ilvN"
                     /locus_tag="VI359_06200"
     CDS             complement(1589..2107)
                     /gene="ilvN"
                     /locus_tag="VI359_06200"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY6973878.1"
                     /translation="MEHIISVTVENKFGVLSRIAGLFSGRGFNIESLSVAPTLDSSMS
                     QMTIVTSGDDRIIEQIVKQLNKLIDVIKVVDLNESDFVSRETALIKVHTRTEDRAEAL
                     RIADIFRANVVDSTPATYTIEVTGDPKKIEAIINLLQPLGIKELTRTGRVAVAREPIR
                     SASAQPKKVVRE"
     gene            complement(2160..>3305)
                     /locus_tag="VI359_06205"
     CDS             complement(2160..>3305)
                     /locus_tag="VI359_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6973879.1"
                     /translation="GGGVIFSGASQELRELAEMTQIPVDMTLMGLGAFPGDHPLSMGM
                     MGMHGTYEANMAVHYSDLVIAIGARFDDRVTGKVSEFCPYAKIIHVDIDPTSIRKNIH
                     VDIPIVGDCKAVLRELNQILRATVNGEQKELRKPWWDQIREWQQAHPLTYQQEVDGPI
                     KPQHVVRRLYELTKDRDPIVSTDVGQHQMWAAQHFKLAQPNRWLTSGGLGTMGFGLPA
                     AMGAQAAFRNRLVLCIAGDGSVQMNMQEMATAVVHKLPVKVVILNNRFHGMVRQWQDL
                     FYNGRYASSYLDTTPDFVKLADAYGAVGLRANKVGDLDNVLKEAIAVDKPVIVDVPTY
                     PYENCYPMIPAGGCNHEMILEDPPELKKKQSAAGKVLPEDKDTVLTA"
BASE COUNT          710 a          991 c          837 g          767 t
ORIGIN      
        1 cagcagacga tacgccgcgc aatgagcccc gcaacttgga cacagaggac tctcgcaccc
       61 tgcgcagttc ggaggaacag agcattttgt tcgttcttca acgtcgcctc cggtttgctc
      121 gccaccaaaa atgctcctgg ctgatactga atgtcttcaa ccactccttc acacggaatg
      181 cgattcacat gcacatcgag gacgttcaag aaaatggtga ggcgaacgct tcgatcctta
      241 ataaatcggg gttcaaactc ttcctcgatg gcgatcactg tgccatcacc aggcgcgaca
      301 accagtccag gtccttgagg aatgactctc gttggatttc tgaaaaacca ggccgtgaag
      361 acagtaaggc ccccggccac cactgcaacg accgtccacc ccaaataccc tgcaagtagt
      421 gtaacgccgg ccccgatccc aacgaaggga attccctctt tgacgatcgg cacgccaacc
      481 gcacggtccg ccacaggctc ctcgatcctc agtttttact cttatcgacc agctggtctt
      541 tcttgagcca gggcatcatc gcccgcagct tggctccgac cgcttcgatg ggatgggctt
      601 cgcctttggc aagcagcgca ttgtatacgg gacgattggc ttgattttcc aggacccatt
      661 ccttggcgaa tcgaccactc tgaatttcgt cgagaattgt tttcatttct tgcttcgtct
      721 gttccgtgac tacgcgaggg ccgcgggtca cgtcgccgta cttggctgtc gtactgatgg
      781 aataacgcat attggcgatg ccgccttgat agatcagatc gacgatcaac tttacttcat
      841 ggagacattc gaaataggcc atctccggag agtagcctgc ctcgacaagg gtttcgtatc
      901 cggcttgaat gagggacgtg aggcctccgc agagcacgac ctgttcaccg aagagatcgg
      961 tttccgtttc ttcacggaag ttcgtctcga tcacgccagc tcgtccgccc ccgatggcac
     1021 tggcataggc taaccccacc tgccgcgtgg tgccgctcgg atcttggtga atggccaaca
     1081 agcagggcac tccactgccc ttcgtatatt cggatcggac gaggtggcca gggcctttgg
     1141 gcgccaccat gaacacgttc acggtggccg gcggcacgat ttgaccgaaa tgaatgttga
     1201 atccgtggcc gaaggcaaga tacgcccccg acttgagatg tggtgcgata tcctgccgat
     1261 agatcgtcgc ttgcgcctca tccggcgcca agatcatcac aacatcggac gccttcaccg
     1321 catcggcgac aggcatgacc ttgagaccgc tctgctcagc cttcttccag gacgcgccct
     1381 ctcgcaaacc gatgaccaca tccacgccgc tctctttcat attgagtgca tgggcatggc
     1441 cttggctgcc gtagccgatg acagcgactt tcttgcttcg aatctgttga aggtctgcgt
     1501 ccttatcgta ataaatcttc atcatccact ccttactgag acatatcctg ttgagagtgc
     1561 tgccggagta ccgaagaacg tcgaagtgct attcgcgaac caccttcttg ggctgggccg
     1621 acgcggaccg gatcggttcg cgcgcaacgg ccacccggcc ggttcgcgtg agttctttga
     1681 tgccgagagg ctgcaataga ttgatgatgg cttcgatctt cttggggtct ccagtcactt
     1741 cgatggtata ggtcgctggt gtcgagtcga ccacattggc gcggaagatg tccgcgatcc
     1801 gcaaggcctc cgcccgatct tccgtccgcg tatgaacctt aatgagcgct gtttcccgcg
     1861 atacaaagtc actttcgttc agatccacca ccttgatgac gtcgatcaat ttgttcagtt
     1921 gtttgacgat ctgctcgatg atccggtcat cacccgaggt aacaatcgtc atctgcgaca
     1981 tcgatgaatc gagcgttgga gccaccgaga ggctctcgat gttgaatcct cgaccgctaa
     2041 agagcccagc aatccgggac agcaccccga acttattttc cacggtgacg gagataatat
     2101 gttccatatg cgatcagtgc tgaagggtga gcggatccat ccaaccctca gcacttccct
     2161 tatgcagtca gtacggtatc cttgtcttcc ggtagaactt tccctgctgc agattgtttc
     2221 ttctttaatt ccggtggatc ttcgagaatc atctcatggt tgcagccgcc ggctggaatc
     2281 atgggatagc agttctcata gggataggtc ggcacatcga cgatcaccgg tttgtcgacg
     2341 gcaatggctt ccttcagcac gttatcgaga tctccaacct tgttggcccg gagacccact
     2401 gcaccgtagg catcagccag cttcacaaaa tccggcgtcg tatccagata gctggaggca
     2461 tatcggccat tgtagaaaag gtcctgccac tgacgcacca ttccgtgaaa ccgattgttg
     2521 aggattacga ccttcaccgg cagcttgtgg acgaccgcgg ttgccatctc ttgcatattc
     2581 atctgaacgc tgccatcccc ggcgatgcaa agcaccagcc tgttgcggaa cgccgcctgt
     2641 gcacccatgg cagcgggcaa tccaaaaccc atcgtcccaa gcccaccaga ggtcagccag
     2701 cgattcggtt gtgcgagctt gaaatgctgg gcggcccaca tctggtgctg gcccacatcc
     2761 gtggacacga ttgggtcccg atcctttgtc aattcataca gccggcggac cacatgctgc
     2821 ggcttaatcg gcccatccac ctcctgctga tatgtcaacg ggtgcgcctg ctgccactcg
     2881 cgaatctggt cccaccaagg cttgcggagt tctttctgtt caccgttaac ggtcgcacgc
     2941 aaaatctgat tcaactctcg aagcaccgct ttgcaatctc ccacaattgg aatgtccaca
     3001 tgaatgttct tccgaatgga cgtggggtcg atatccacgt ggatgatttt ggcataggga
     3061 cagaattccg acaccttgcc cgtcacccga tcatcaaacc gcgctccaat cgcgataacg
     3121 agatcggaat agtgcacggc catgttggcc tcatacgttc catgcatccc catcatgccc
     3181 atggacaacg gatggtcgcc ggggaacgca cccaatccca taagtgtcat gtcgaccgga
     3241 atctgtgtca tttcggcaag ctcacgcaat tcctgtgagg caccggaaaa tatcacgccg
     3301 ccacc
//
LOCUS       DATDNW010000255         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_916442, whole genome shotgun sequence.
ACCESSION   DATDNW010000255 DATDNW010000000
VERSION     DATDNW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916442"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VI359_06210"
     CDS             <1..490
                     /locus_tag="VI359_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6973880.1"
                     /translation="DLAQHGDRPVIGVDWADADAYCRWKGKRLPTEAQWEKAARGTDG
                     RLYPWGNQVPANTLANFALGARFSYGQVLMPVRSYEQGKSPYGLYQMAGNVWEWVQDW
                     YAANYYEMSPERNPQGPEQGQFKVLRGGSWSDLPKYLLTYGRFKLPPETRNSYTGFRC
                     AK"
     gene            complement(820..1047)
                     /locus_tag="VI359_06215"
     CDS             complement(820..1047)
                     /locus_tag="VI359_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973881.1"
                     /translation="MWSANSDSDLAGYKVYVGTQSGLYTFPGSPFTIGSITSYTIINL
                     PPGQTYFFALSAYDTSGNESPLSVEVSKSIY"
     gene            1046..1201
                     /locus_tag="VI359_06220"
     CDS             1046..1201
                     /locus_tag="VI359_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973882.1"
                     /translation="MSVALPVVDEDGGGVVDGGGEVEDGGADGGEGGGLVTVGAGGCS
                     KLIIAGF"
     gene            1754..1999
                     /locus_tag="VI359_06225"
     CDS             1754..1999
                     /locus_tag="VI359_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973883.1"
                     /translation="MTTTWSPVDEHIVQRICWNWRNELSVFIGRDPDLGKHRGARHRH
                     AGRVYLHCAYCLRDNRDTPEELQFVTRMDEQKQRTNV"
     gene            complement(1989..2438)
                     /locus_tag="VI359_06230"
     CDS             complement(1989..2438)
                     /locus_tag="VI359_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973884.1"
                     /translation="METESTHHTLTMLYPLYKEEVYRRREQMMTLTAWGAIGLLAMLF
                     AILLNPSKAQLTTPETWVIGFAALIWSSLFCSFIAQQRYRHRLAKQILIQLERALGFY
                     EEGLFVEHRALYPKGWQTEWLGDKSTILYFACLGVLTGLIVLALLTH"
     gene            complement(2469..>2729)
                     /locus_tag="VI359_06235"
     CDS             complement(2469..>2729)
                     /locus_tag="VI359_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6973885.1"
                     /translation="LRYGFYEDCLRHIAYGPAEQITAPTLIVQGDRDEFVPLHQSQRL
                     LDAVRVPKRLDLLPGADHQFTRGEDFRTMTISIVEWLVRHVN"
BASE COUNT          613 a          666 c          870 g          580 t
ORIGIN      
        1 cgatttggca cagcatgggg accggccggt gatcggtgtg gactgggcag atgctgatgc
       61 ctactgccgc tggaagggaa agcgactccc gaccgaggcc caatgggaga aggcggcgag
      121 agggacagat gggcgactct acccatgggg taatcaggtt cccgccaata cgcttgcgaa
      181 ttttgccttg ggtgcgcgat tcagttatgg ccaagtcctc atgccggtgc gttcgtatga
      241 acaagggaag agtccgtatg gcctctatca gatggctgga aatgtgtggg aatgggttca
      301 agattggtat gcagcgaatt attacgagat gtcgccggaa cggaatcctc agggaccgga
      361 gcagggccag ttcaaggttt tgcgcggcgg ctcatggtcc gacctgccga agtacctcct
      421 cacctacgga cgattcaaac ttccgcctga gacgcgaaac agttataccg gtttccgctg
      481 cgccaagtag ctcaaaacaa agtgctgagt gctgagaact gagtgctggg tgtagagtgc
      541 cgcacaagtg acgattccgc agtcctaacc ctttacccct aatcccttgc ctctaaccaa
      601 gtgaattttt cggctgctcg aaccgtcctc cgacaaggcc gcagggagtc cggcgactga
      661 ggcgtaccct tctcaccctc cctccccgag ctgttagaac agctcgttcc cgagtgggta
      721 cgtcgcaagg agagggacag ccgagaacga agcctgggtc aaggtgcgtc ttggcgcacc
      781 agggcgggcg ggtgagaagg aaggcggttt cagcagccgt tagtagatgc tcttgctgac
      841 ttcaacagag agtggacttt cgttgccaga ggtatcgtag gccgagagag caaagaagta
      901 ggtctgccca ggaggtagat tgataatggt ataggaggta atgctcccga tcgtgaaggg
      961 tgaaccggga aatgtgtaga gcccggactg cgtgccgaca tacactttat atccggctag
     1021 atctgagtca ctattcgcac tccacatgag tgtggcgctc cccgtggtcg atgaagacgg
     1081 tggaggggta gttgacggag gaggtgaagt ggaagacggc ggagcagacg gcggcgaggg
     1141 aggagggctc gtaacggtcg gagccggtgg ttgcagcaag ctcataatag ccgggttctg
     1201 aagcaggttc gctgccgagc gggatccgct tatcgtgggg gctgctgtag ttcctgccgt
     1261 agtgccagcc aaggagcttg aacttatggt ggatgggctc gcgagagaag aagacgagag
     1321 ccgagtcgag gctgtcgttg gagcgacggt ggctgatgca gatccttgga aggtcgacga
     1381 tggttttaca acagttccat ctccgaggcc ggatgatccg gttgatgtgg cggtcgagag
     1441 agacgcgctt tggacggcag ccttcggttt tgcaataagc ggttgcgtcg tgggtggaga
     1501 aggaatgggt gtcacagttg cgattccagg acgtttcgtc agtttatggt gcggaggaag
     1561 tatgcttggt atggaaggcg aagggccctg cggtcgagca gctgaattca gaacctgagg
     1621 aggctgagta tcgcttccgg agacaatcac accctgagtc gaggagattg ggggcaccgt
     1681 gccggttgtc ggacgatgga gcaagctgtg acggcgtccg ttgtcctgat cagccgcgta
     1741 cagcacggtt gtcatgacga caacgtggag tccggtcgac gagcatatcg tccagaggat
     1801 ttgttggaat tggcgcaatg agctatcggt tttcatcggg cgggatccgg atcttggcaa
     1861 acatcgcggt gctcggcatc gtcatgccgg ccgcgtgtat ctgcactgcg catattgtct
     1921 gagagataac cgcgatacgc cagaagaact tcagtttgtg acgcgtatgg atgagcagaa
     1981 acagcggact aatgtgtgag cagggcgagg acgatcaaac cggtcaagac gccaagacaa
     2041 gcgaaataga ggatggtgct tttgtctccc aaccactcag tctgccatcc tttgggatag
     2101 agggctcggt gttcaacaaa caagccttcc tcgtagaagc ccagcgcccg ttcaagttga
     2161 atgaggatct gcttggccaa acgatgccga tatcgctgtt gtgcgataaa tgagcagaag
     2221 agactagacc atatcagtgc cgcgaagccg atcacccagg tttcaggagt cgtcagctgt
     2281 gccttggagg gattcagcag gatggcgaac agcatcgcga gaagtccgat ggcaccccaa
     2341 gccgtcagcg tcatcatctg ttcacgtcga cggtacactt cctctttgta caacggatac
     2401 agcatggtca gcgtatggtg cgtactttct gtctccatgg attccacgtg ggatctttcg
     2461 cgtcctgttc agttcacatg ccgaacaagc cactcaacaa tcgagattgt catggttcga
     2521 aaatcctcac cccgcgtgaa ttgatggtct gctccgggca acagatccaa tcgcttgggg
     2581 acacgtaccg catcgaggag tcgctggctc tggtgtagcg gaacgaactc atcccggtca
     2641 ccctgcacga tgagagtcgg cgccgtaatt tgttcggccg gtccataggc gatgtgacga
     2701 agacaatcct catagaaacc gtatcgcag
//
LOCUS       DATDNW010000256         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_374601, whole genome shotgun sequence.
ACCESSION   DATDNW010000256 DATDNW010000000
VERSION     DATDNW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374601"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..741)
                     /gene="pal"
                     /locus_tag="VI359_06240"
     CDS             complement(85..741)
                     /gene="pal"
                     /locus_tag="VI359_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6973886.1"
                     /translation="MYGKTFGSGAIAVMGLILIAGQGCSSKWWQSDGDTETGQSANGQ
                     SGNSQLPTISGGGSSGELSGFSQNPSEERLQPGGGITSVSSSGMSERQRAELTKEEKA
                     AIEAGLQDVFFGYDQWVISDSGMNALNRDAEWLKTHPGAVMKVEGHCDERGTGEYNFV
                     LGEKRAKAARSYLVEMGVSPKQIAIVSYGKERPFCAEHDEACYQQNRRGHMLLSVKKN
                     "
     gene            complement(939..1085)
                     /locus_tag="VI359_06245"
     CDS             complement(939..1085)
                     /locus_tag="VI359_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973887.1"
                     /translation="MNPVILHEVRGGFFLLVGFAMLAFICALAALGTVMRPIRWADRR
                     SAHF"
     gene            complement(1465..1541)
                     /locus_tag="VI359_06250"
     tRNA            complement(1465..1541)
                     /locus_tag="VI359_06250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Arg,seq:tcg)
     gene            complement(1683..1874)
                     /locus_tag="VI359_06255"
     CDS             complement(1683..1874)
                     /locus_tag="VI359_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973888.1"
                     /translation="MSEVFPIPQPSSNRQIYSPNYGTVQSAKMIADRFTGQSRGFTFV
                     EMVTSEEAQKAIASLHGTD"
     gene            complement(2118..>2523)
                     /locus_tag="VI359_06260"
     CDS             complement(2118..>2523)
                     /locus_tag="VI359_06260"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY6973889.1"
                     /translation="AEIVKRAKQSIPEIVAKYGWEYFRDLESTVCRDLADRDQVVIDT
                     GGGAVLRPQNVEVLKRNGKLIWLTATPDTITARIGGDTQRPSLTGTKSFLEEITEVLR
                     DRTPIYQAAANHVIKTDGRSIQQLVEAVLALM"
BASE COUNT          610 a          720 c          595 g          598 t
ORIGIN      
        1 atcacggaat gacgcgaaat aaagaacccc ggtgcctcgt gtggttaggt gcaccggggg
       61 ttcgtaatac ctacagttac gatcttagtt cttctttacg gacaatagca tgtgcccacg
      121 gcggttctgc tgataacagg cttcgtcatg ttcggcacag aagggccgct cttttccata
      181 cgaaacgatg gcaatttgct ttgggctcac tcccatctcg accaaatagc tcctcgcagc
      241 tttggcccgc ttctcaccga gcacgaagtt gtattcgccc gtgccccgct catcacaatg
      301 tccttcaact ttcatcaccg cacccggatg ggtctttaac cattccgcat ctcgattcaa
      361 ggcgttcatg ccagaatctg aaatcaccca ttgatcatat ccaaaaaaga catcttgaag
      421 ccccgcttca atcgcagcct tttcttcttt agtcagttcc gcacgttgcc gttcactcat
      481 tcctgacgaa gacaccgaag tgatcccacc gccaggttgc aaccgttctt cagaggggtt
      541 ttgtgagaag ccgctcaact ctccgctgga tcctccccca gatatcgtgg gaagttgcga
      601 attgccgctt tggccatttg ccgattgccc agtctcagtg tccccatccg attgccacca
      661 cttggaactg catccctgtc cggcaataag aatcaaaccc ataactgcaa tagcacctga
      721 tccgaatgtt tttccgtaca tggttcctct ccaattcctt tcagtgaata atctgatgca
      781 acaaatatag caccagaagc gaaaactcgc ggcaaggaga ggcttggatg tttattgaga
      841 aatattagat acttagaatg atcagtggcc agtgggagcc tgcaatgtgg ggggaggctt
      901 taccaaaact caagagcaag tgcgatctga ccagcctgct agaaatgggc ggaacggcga
      961 tcagcccatc gaatagggcg catgactgtc cctagagccg ccaaggcaca aatgaacgcg
     1021 agcatggcaa aacctactag caggaaaaaa ccacccctga cttcgtggag gataaccggg
     1081 ttcatgggat atccctcctc ttggtacctt tgtcgactac ccgctctgcc atccgcagga
     1141 acaaacccac cgagctacag gatcgtgctt cgtgagccaa cgtccacagc aggggcagtg
     1201 ccgtgtccaa gccagtcgtt ctccgtgacc agtggacctt tcgccaaggg agcacgccgc
     1261 ctctgattta aaggatagca ccatcgtaga ctccgtcatc ttctgcacgg ttgtacgcta
     1321 aacaattatt agattatcgc tggaagattg aagagtctat gcagctttgg tgggggagtt
     1381 catccgtcga agtgagtaag acagtattca caacttattg atattacagc actcacgaag
     1441 gtttcttaca agatagcaag taaatggtgc gcccggcagg aattgaacct gcgacctgcg
     1501 ggttcgaagc ccgacgctct atccaactga gctacgggcg cacagggtta cttcaagcac
     1561 tctcggtgag atcctgtcaa ggagatatcc tggtcgatac taccatcgac ctcgatcgcc
     1621 gccaccacca aacgatcgct tctcctgagg acgggcttca ttgacgacaa gcgtccgccc
     1681 acctaatcag ttccgtggag cgacgcaatc gccttctgtg cctcctcgga cgtgaccatt
     1741 tcgacaaatg taaaccctcg cgactggccg gtaaatcgat cagcgatcat ttttgcggac
     1801 tggacagttc cataattggg agaataaatc tgtcgattgc tgctcggttg tggaataggg
     1861 aagacttccg acatacaact tgtttcccat ccgccacccc catcacggct gcgacattga
     1921 cttgagaaga gagcagcgag gaaggacagc caattaaacg gaccactgtc ggtcggtcac
     1981 acgttccggt ccgctttcct tcgccaaggc aatatcgatc aggacttctc tacgtcagct
     2041 agcacactcg gcccctgagt cagaatcagc atggaaaaac atatctctcg attccggcga
     2101 gttcaattcc ccatccctta catcaaagcg agaacggctt ccacaagctg ctgaatcgat
     2161 cggccgtcag tcttgattac gtggttcgct gccgcttgat atatcggcgt acggtctcgc
     2221 aacacctcgg tgatttcctc aaggaacgat ttggttcctg ttagcgatgg acgttgagta
     2281 tcgccgccga tccgtgccgt aatcgtatcc ggcgtcgcag tcaaccagat gagcttgcca
     2341 tttcgcttca acacttcgac gttctgaggt cgtagaactg cgccaccccc agtatcgatc
     2401 accacttggt cacggtctgc aagatcccga cagacggtgg actccaaatc acgaaagtac
     2461 tcccatccat atttggccac gatttccggg attgactgct tggctcgctt gacaatctcc
     2521 gca
//
LOCUS       DATDNW010000257         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1064054, whole genome shotgun sequence.
ACCESSION   DATDNW010000257 DATDNW010000000
VERSION     DATDNW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064054"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VI359_06265"
     CDS             complement(<1..644)
                     /locus_tag="VI359_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6973890.1"
                     /translation="MGSYNRNGVARTMSAARTPLTPETDAQIRAIGSTDILVGIPSYN
                     NAGTVGHVVQAVSAGLAKYFPDRRAVLVNSDGGSSDKTRDIVASTVIDHNHLFISDQQ
                     SVLHRIITPYHGIPGKGSAFRTIFEIARQLNAKACAVVDADLRSIAPEWIELLLRPLL
                     DEGYDYVAPYYLRHKYDGTITNSLAYPLTRALYGQRIRQPIGGEFGFSGSLAAH"
     gene            complement(605..1849)
                     /locus_tag="VI359_06270"
     CDS             complement(605..1849)
                     /locus_tag="VI359_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY6973891.1"
                     /translation="MSDFYQNGVVTVLHRLGSPNVDQLEGELERCGRMHPIALVLPSL
                     YAELERPALKKIVETLKTITYLHEIVISLDRASALEFRLAKQYFANLPQRVRLVWNDG
                     SRIQTLLNLLVSQNIDIGLPGKGRGCWLAFGYVLARNHSQVIALHDCDITSYHREYLA
                     RLCYPVANPNLGYEFCKGYYSRVTDRLHGRVTRLFITPLIRSLQQLLGPHALLTFLDS
                     FRYPLAGEFAMVRDLAWINRIPGDWGLEIGVLAEVYRNCALRRICQADIADAYDHKHQ
                     SLSSQDPDAGLLKMCVDITKSLFRNLASEGVVLSDGILKTLRATYLQAAQDAISRYEN
                     DAAINSLTFDRHEERTAVEVFLKGMKLATEGFLEDPLGVPMISNWSRVTHAVPDIFTR
                     LIEAVEEDHAWDPTTETVSPGR"
BASE COUNT          488 a          576 c          631 g          458 t
ORIGIN      
        1 taatgggcgg cgagggaacc ggaaaaacca aactcgccgc cgatcggctg acggatgcgt
       61 tggccgtaga gcgccctcgt caagggatag gcgaggctgt ttgtaatcgt tccgtcgtat
      121 ttgtgccgca gatagtaggg cgcaacatag tcgtatccct cgtccagcaa cggacgcaac
      181 aacaactcga tccactccgg cgcaatactg cgcaagtccg catcgaccac cgcacacgcc
      241 ttcgcattga gctggcgcgc aatctcgaag attgtccgaa acgcactccc tttccccggt
      301 attccgtggt agggagtgat gatgcggtgt aacacgcttt gctgatcgct gatgaataaa
      361 tgattatgat cgatcaccgt gctggccacg atgtcgcggg tcttgtcgga ggagccgcca
      421 tccgaattga ccagcacggc gcgccggtcg ggaaagtact tcgctaaacc cgcgctcacc
      481 gcctgcacga catgaccgac ggttccggcg ttgttgtagc tcgggatccc gacaagaatg
      541 tccgtggatc cgatggctcg gatctgcgca tccgtttccg gtgtgagtgg ggtccgcgcg
      601 gcgctcatcg tcctggcgac accgtttcgg ttgtaggatc ccatgcgtgg tcctcttcaa
      661 ctgcttcgat gaggcgggtg aagatatcag gcaccgcatg ggtgacgcgg ctccagttgg
      721 agatcatcgg cacacccagt ggatcttcga gaaacccctc cgtcgcgagt ttcatccctt
      781 tcagaaacac ctcgacggcc gtccgttcct catgacggtc gaaggtgagg ctgttgatgg
      841 ctgcatcgtt ctcatatcgg ctgatggcgt cttgagcagc ttgaagatag gtcgcccgca
      901 aggttttcag gatgccgtcc gacagcacca cgccttcgct cgctaaattc ctgaagaggg
      961 acttcgtgat gtcgacgcac atttttaaca acccggcatc tggatcctga cttgagagag
     1021 attggtgttt atggtcatag gcgtctgcaa tgtcggcctg acagatacgc cgcaacgcgc
     1081 agttgcggta cacctccgcc agcacgccga tctccaaccc ccaatccccg gggatgcgat
     1141 taatccacgc caggtcgcgg accatggcga attctccagc gagaggataa cgaaagctgt
     1201 cgagaaaggt caggagtgcg tggggtccca ggagctgttg gaggcttcga atcagtgggg
     1261 tgataaagag ccgtgtgact cgaccatgca gacggtcggt gactcggcta tagtagcctt
     1321 tacaaaattc ataaccgagg ttcgggtttg caacaggata acagagacgt gccagatact
     1381 cgcggtgata gctggtaata tcgcagtcgt gcagggcgat gacctgacta tgattgcgcg
     1441 ccagcacgta gccgaacgcc agccaacagc cacgtccttt gcccggcaat ccaatatcaa
     1501 tattctgtga gacgagcagg ttgaggagtg tctggatgcg ggagccgtca ttccagacga
     1561 gccggactcg ctggggaaga ttagcgaagt actgtttggc caggcgaaac tctaacgctg
     1621 acgcccggtc gagtgaaatc acgatttcat gaaggtatgt gatggtcttt agggtttcga
     1681 caatcttttt gagagccggt cgttccagtt cagcgtagag agacggcagc accaaggcaa
     1741 tcggatgcat acgtccgcag cgctctaact cgccctccaa ctggtcgacg ttggggctgc
     1801 cgagccggtg gagaacggtc acgacgccat tttggtaaaa gtccgacatg cggaactcca
     1861 gtcagaactc gaagggtcac cctttagagg acgtgcacag agaagaataa gaagcggtta
     1921 cgacagccag tggataaata cggatttagg atacctgaat tacacagggt gtgccatatg
     1981 aaatttaatg aaaacccagg cctcggcagt ttccggtttc agagccgggg tattgcctag
     2041 tggcaaaagc cttcacttat acagatgaat cagatgcacg tcatggcctg tcggtagaca
     2101 ggcgcttcgc gagacggtgg gccaatcgct tcatgccggc agaacgatcg gca
//
LOCUS       DATDNW010000258         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1194758, whole genome shotgun sequence.
ACCESSION   DATDNW010000258 DATDNW010000000
VERSION     DATDNW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194758"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VI359_06275"
     CDS             complement(<1..467)
                     /locus_tag="VI359_06275"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEY6973892.1"
                     /translation="MITVWRNSCLNFTVLLVAVLSFTVAVTDVAAGSLPKIKILATGG
                     TIAGAQAEGQDAGYKAGSFKVEDLIKAVPQLKDLADITGEQVVNIGSQDMNNEVWLKL
                     AKRANEVLAQGDVDGIVVTHGTDTMEETGYFLNLVVKSDKPVVMVGSMRPATA"
     gene            complement(515..2155)
                     /gene="aspD"
                     /locus_tag="VI359_06280"
     CDS             complement(515..2155)
                     /gene="aspD"
                     /locus_tag="VI359_06280"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HEY6973893.1"
                     /translation="MNQLPRTLKTSRATQRALSKLSPFELKDNLIHLSKETARQGAAT
                     MLNAGRGNPNWIATEPREAFFLLGQFALGESRRVWDEKILAGMPAKAEIAQRFQTFLG
                     TCRGQPGEAFLRKMYQYGVEQKGFNADAFIHEVADSIIGDNYPVPDRMLVHIEHIVHD
                     YLMKEMCDGRTPKGKFDLFAVEGGTAAMCYIFDSLMQNGLLTRGDTIGLMVPTFTPYI
                     EIAEMDRYAFKIVRVNASDTRGDGSHTWHYPNAELDKLANRKIKLMVCVNPSNPPSLA
                     LSKAELKRIVQIVTRKNPNLMIVTDDVYGTFVPHFRSLMAELPANVIGVYSFSKNFGC
                     TGWRLGVVAVHEQNVLDRMIAKLPPAWKKRLARRYGSLTLEPEKIKFIDRMVADSRSV
                     ALNHTAGLSLPQQVQMAFFALSHLFDREDIYKNLTMKIVQRRRDLLWQGLGLPLPPPD
                     PDRAWYYVELDLEVWAKHMVGEGFFEFLSKNYEPVDFLFRIAERSSVVLMDGGGFGGP
                     KWSIRVSLANLNDGDYTRIGEYMMSAAQEYIAAWKASR"
BASE COUNT          502 a          722 c          645 g          559 t
ORIGIN      
        1 atggcggtag caggccgcat ggatccaacc atgacgaccg gcttgtcgct cttcaccaca
       61 agattcaaga aatagccggt ttcctccatc gtgtcggttc catgggtcac gactatgcca
      121 tccacatcgc cctgcgccaa cacttcgttt gcgcgtttgg cgagcttgag ccagacctcg
      181 ttgttcatgt cctggctgcc gatgttgacc acttgctctc cggtaatatc ggccagatct
      241 ttcaactgcg gaactgcttt gatcaggtct tcgactttga agctgccagc cttgtagccc
      301 gcgtcctgtc cttctgcctg agcacccgca atagtgccgc cggtggccag aattttgatt
      361 ttgggaagac tgccggcggc aacatccgtc actgcaacag tgaatgaaag aaccgcaacc
      421 aacaaaaccg tgaaattcaa gcagctattc cgccatacgg tgatcatgct tgcctcctcg
      481 ttgactcaca tccattgatg ctttcctgct tcgtctatct gcttgccttc caggcagcga
      541 tatattcctg agccgcactc atcatgtact caccgatcct cgtgtagtca ccgtcgttga
      601 ggttcgccag cgagacgcga atggaccact tgggtccacc aaagcctcct ccatccatca
      661 ggacgaccga cgaacgctcg gcgatccgga acaggaagtc gaccggttcg taattcttac
      721 tgaggaattc aaagaagccc tccccgacca tgtgtttcgc ccagacctcg agatcgagct
      781 ccacgtagta ccaggcacgg tctggatcgg gaggcggaag cggaagccct agaccctgcc
      841 agagcaggtc gcggcgccgc tgaacgatct tcatcgtcag gttcttgtaa atatcctccc
      901 tatcgaacag gtgggagagt gcgaagaagg ccatctggac ctgctgcggc agcgacaacc
      961 cagccgtatg attcagtgca acgcttcgac tgtcggcgac cattcgatcg atgaacttga
     1021 ttttctcggg ctcgagcgtg agtgatccgt accgcctggc gagcctcttc ttccatgccg
     1081 gcggaagttt cgcgatcatt cgatccagga cgttctgttc atgcaccgcg accacaccga
     1141 gccgccaacc ggtgcagccg aaattcttcg agaacgaata gacgccgatc acattcgccg
     1201 gaagctcggc catgagcgag cggaagtgcg gcacgaacgt gccgtacacg tcgtcggtga
     1261 cgatcatcag attgggattc ttgcgcgtaa caatctggac gatccgcttc aactcagcct
     1321 tggatagtgc gagcgacgga gggttgctcg gattcacgca caccatcagc ttgatcttcc
     1381 ggttcgccag cttgtcgagt tcggcgttgg gatagtgcca ggtgtggctg ccgtcgccgc
     1441 gagtatcgga cgcgttcact ctgacgatct tgaacgcata tcggtccatc tccgcgattt
     1501 cgatgtaagg cgtgaacgtc ggcaccatca ggccgatcgt gtcgccgcgc gtgagcagcc
     1561 cattctgcat gagagaatcg aagatgtagc acatcgccgc cgtcccacct tcgacagcga
     1621 agagatcaaa tttgcctttg ggcgtgcggc catcgcacat ctccttcatc agatagtcgt
     1681 gcacgatgtg ctcgatgtgc accaacatcc ggtccggcac cgggtagttg tcgccgataa
     1741 tggagtcggc gacttcgtgg ataaaggcat cggcgttgaa gcctttctgt tccaccccgt
     1801 actgatacat cttcctcagg aatgcttcac ccggctgccc ccggcaggta ccgaggaaag
     1861 tctgaaagcg ttgcgcaatt tccgcctttg ccggcatgcc cgccagaatc ttttcatccc
     1921 agacacgccg actttcgccg agcgcgaact gcccgagtag aaagaacgcc tcgcgcggct
     1981 cggtcgcgat ccagttcgga ttgccacgac cggcgttcag catcgttgcg gcgccctgtc
     2041 gcgcagtctc ttttgacagg tggatcagat tgtccttgag ttcgaagggg gagagtttgg
     2101 agagcgccct ctgcgtcgcg cggctggtct tcagtgtgcg gggaagttgg ttcatctcag
     2161 cctccgtctg tggatgagtt caaatgccat ggcgtcggcg cgaacccaat atgccctgat
     2221 ctcacatgta ctcatcgttt cagaccctag gaggtgaaaa gttttgtaag gctccttcaa
     2281 ggacatccag gggaaacgca tttggagaaa tatctcggtt gaactcatca tagggcgttt
     2341 tagcctgctc ctgatcaatc aagattattc cccccttcgt ctgacccgat tcctcatttc
     2401 atcatcagca cgatgatgac cccccaga
//
LOCUS       DATDNW010000259        13224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_239429, whole genome shotgun sequence.
ACCESSION   DATDNW010000259 DATDNW010000000
VERSION     DATDNW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13224
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239429"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VI359_06285"
     CDS             complement(<1..629)
                     /locus_tag="VI359_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY6973894.1"
                     /translation="MELIQPTKQGQLAMSKTGKRLLLVDPYPRNNPYHLTASERRAVW
                     FPKLSLPTIAAYTPENWDVDLVDEAVEDIDFDYPCDMVGLSIMTCYAPRAYEIATEFR
                     KRGRTIVMGGVHPTYCPDEALQYADAIVCGEAEDLWPQLVADYEAGKMQRMYKMTSFP
                     SLDHYKSPRVELLSPDAYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRR"
     gene            complement(690..962)
                     /locus_tag="VI359_06290"
     CDS             complement(690..962)
                     /locus_tag="VI359_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973895.1"
                     /translation="MSQSREIMCRTAALLFGIAGLLAACGVVGPPIPPEDVGVTPTIT
                     RQKLQHAGEQQKQEAAVAEETPQDATEPALQGPDVELPPLRPVGTR"
     gene            complement(971..2383)
                     /gene="argH"
                     /locus_tag="VI359_06295"
     CDS             complement(971..2383)
                     /gene="argH"
                     /locus_tag="VI359_06295"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY6973896.1"
                     /translation="MAQRKGRTGRRAVADKAWGGRFRAKTHRLVEAFSVSVGIDRRLY
                     AQDVQGSVAHCKTLEKARVLTRGETRTIIRGLESVKAELDRGRFRFLSSDEDIHMAIE
                     RRLTELIGHLGGKLHTGRSRNDQVALDIRLYLREELDHLAGQLNEFQRVVLAKAKANR
                     TVAMPGYTHLQRAQPVLVAHHLLAYVEMLERDKERVRDARARLNVMPLGSGALAGSNY
                     PIDRRYTASLLGFPTITQNSLDAVSDRDFMIEVASVLAIIMMHLSRLSEELILWSSQE
                     FHFVDLPDAFCTGSSMMPQKKNPDVPELIRGKTGRVYGQLMNLLTMMKALPLSYNRDL
                     QEDKPALFDALDTVQTSVQVLTELMRRVKINRDALRHAISGGGLLATEVADYLVTRGV
                     PFREAHAITGRVVRAALDQERDLTDFSLEELQAFSNRFEKSVTSRLTVMAAIDRKAQI
                     GGTARTQVDRRIKELERMLS"
     gene            complement(2390..3601)
                     /locus_tag="VI359_06300"
     CDS             complement(2390..3601)
                     /locus_tag="VI359_06300"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY6973897.1"
                     /translation="MKRTIKKVVLAYSGGLDTSVILKWLQETYECEVVAFCADLGQGE
                     DLKAVKTKAQKLGVKSVYVEDLRETFVKDHVFPMLRANAVYEGSYLLGTSIARPLIAR
                     RQIEIAKKEGAEAVSHGATGKGNDQVRFELTYMALEPKIKIIAPWREWEFKARRELIE
                     YAERHGIPVTATKAKPYSMDLNLFHVSYEGGILEDPWEAPPEEIFQMTVSPERAPDKP
                     QEIEIEYDAGDPVAVDGKKMSAATLLAHLNKLGGAHGIGRVDLVENRYVGMKSRGVYE
                     TPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELVYYGYWYSPEREVIQKMMDEA
                     QKDVTGTVRVKLYKGNCTVVGRKSKNSLYRLDIATFEEDEVYRQKDAEGFIRLNALRL
                     AIRAQRRKASS"
     gene            complement(3598..4557)
                     /gene="argF"
                     /locus_tag="VI359_06305"
     CDS             complement(3598..4557)
                     /gene="argF"
                     /locus_tag="VI359_06305"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY6973898.1"
                     /translation="MKTVQTMKKDLLDVAAVSKAEIEHLLKLAAKLKDRQRRGILHSL
                     LPGKTLGLLFQKPSTRTRVSFEAGMNQLGGHALVLHMADIQLSRGESVADTARVLSRY
                     LDGIVIRTYDHATVEEWAAEATMPVINGLTDLSHPCQALSDLLTIREKKGRLQGVKIA
                     YIGDGNNVANSLIEATAKMGMTIALGCPIGYQPDQHVVDVARIEAANTGAVIEVGQDP
                     HVAVKEADVIYADVWISMGREREQARRLKVLAPYQVNSRLLHRAKPDAIVMHCLPAHR
                     GEEITAEVLDGPQSVIIDQAENRLHMQKAILVTLLGKGRQKTL"
     gene            complement(4567..5787)
                     /locus_tag="VI359_06310"
     CDS             complement(4567..5787)
                     /locus_tag="VI359_06310"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEY6973899.1"
                     /translation="MPTDELKDDASRYLMQTYARQPLSITRGRGTKVYDLEGREYIDF
                     VGGIAVNILGHGHPDLVHAIQRQAQQLIHTSNLYYTEPQVKLARVLVDHSFADKVFFC
                     NSGAEANEAAIKLARRYSHEKYGDGRYEIITMKNSFHGRTMATLTATGQEKVQKGYDP
                     LVPGFRYVAFNSLAEVEQAVTNKTAAIMLEPIQGEGGVHVADRTCLAGLRALCAQKDI
                     LLIFDEVQTGMGRTGTLFAYEQFGVQPDIMTLAKGLGGGVPIGACLAIDSVAKVFGPG
                     SHASTFGGNPLACAAALAVFRVLLEGRVLDHARRMGEYLAKGLSECKDRHRIVREVRG
                     LGLLQGMELDIDAKTVVSDCLTRGLLINNTSEHVVRFVPPLIITQREIDRLIEVLSQI
                     FSKHAGTEARAHEA"
     gene            5930..6577
                     /locus_tag="VI359_06315"
     CDS             5930..6577
                     /locus_tag="VI359_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEY6973900.1"
                     /translation="MKTCSQCRQPNEADANYCFQCGTTLTTAKTEIETGSPSTTPVPM
                     PDADLWHRFIGPHADRYLEQFKKFGPENNPRFALTWNWPAFLFVSFLWFLYRKMYLYA
                     LVYAIGPMISTYLTGDMTVGIAWSVIAGATANYIYYWHCREQIGEIKKKAWSDPAVQD
                     QSLKEAGGVQPYVIYVGIAFYVLFIMTMIKLIQDGEFDGERSPGKPARPAATGSV"
     gene            complement(6791..7636)
                     /locus_tag="VI359_06320"
     CDS             complement(6791..7636)
                     /locus_tag="VI359_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973901.1"
                     /translation="MTTVGDSANPSRRAVEAWWVTLAERLQVADRARGFFASIQSGHL
                     AERLAQIGGQLDYILFVLLRYWVPTVLPPPGRERLTKNDPDYWLESEQILKASVHRLR
                     ELKPLIELLTTPNPLVEAEVEHGPATSPVAEVELGKMLEGIAEVAGSYGGPDYTSVVK
                     HFDPVPLRQTQPFKHNKKNSAELWVVFLLREHFRSLGLGKDRYWPLLAPICTAAGILN
                     QNGQAYGSDELKSWWQKNWPRTYQQLDQKQEATDSGGAAYEQDFEWFQAWISWQRSQH
                     PVTGW"
     gene            7764..8369
                     /locus_tag="VI359_06325"
     CDS             7764..8369
                     /locus_tag="VI359_06325"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY6973902.1"
                     /translation="MARLILLRHGESQWNLENRFTGWVDVPLSPRGIQEAKNAGEKLR
                     SFVFDRAFTSVLTRAIETLRLVLETIGQVNIPIEKDKALNERMYGELQGLNKADTAKK
                     YGDEQVKIWRRSYDIRPPGGESLKDTADRVLPYYESRIKPYALKGETILIAAHGNSLR
                     ALVMQLEQLSKEQVLELNIPTGAPLLYELDKNGMVVNHRYL"
     gene            8640..9002
                     /locus_tag="VI359_06330"
     CDS             8640..9002
                     /locus_tag="VI359_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973903.1"
                     /translation="MNWTGQFVLYSVMLLATPALADMGKTAEPLKAPPGSKAETHINT
                     GIEHYDMGHWDVAKNHFLEAEKADLQSAEAHYNVALVLDKTGDHVGATTHFKRAKELG
                     KNNADIQNSEILNKHVKM"
     gene            complement(9036..10058)
                     /locus_tag="VI359_06335"
     CDS             complement(9036..10058)
                     /locus_tag="VI359_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY6973904.1"
                     /translation="MKRLRILVLMHEDLVPPDTLSGQEPELAEWKTEYDVVSTLRKLG
                     HEVTPLGVKSDLGVIRKAVEEWRPHIAFNLLEEFDGVAVYDQNVVSYLELLRVPYTGC
                     NPRGLMLARDKVLSKKLFSFHRIPFPEFMVVPQGRTVKRPSRLSFPLIVKSVTEEASL
                     GISQASIVQDDERLKERVAFIHNSVGTGALIERYIEGREFYVGLMGNGHLHAFPVWEL
                     IMDKLPDDARRIATERVKWSRKYQDKYGIRSGEVKNLPECKVEEIQHLAKRVYRALDL
                     SGYARVDLRMDGQGNLYILEANPNPQIAHDEDFADSAERAGYSYKDLLQELLSIGLRW
                     QPAKAA"
     gene            complement(10055..11116)
                     /locus_tag="VI359_06340"
     CDS             complement(10055..11116)
                     /locus_tag="VI359_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY6973905.1"
                     /translation="MQRAPLQAFPGAESEQSEPEWASWPDEKLLDLPMCRLGVSLESG
                     FLSKQIEQLYAELEAKQLRFRPHFWISNEWFTPDGVPGIAVPFYLAHPRLVKLELAQM
                     LEVEGGTPEWCMRILRHEAGHAIENAYRIRRLRSRQQVFGKSSQPYPQYYRPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLTPDSAWIDRYKGWPVLKKLQYMDSLLKGLADM
                     PPKVVTREEVDPIQTIKKTLREHYDRKRSHYGVERPSFYDPDLKRLFSDAAAHADKMT
                     AAAFLKRFRREVRRKVAAWTGEYQYTIDQVLEDMIQRCRELNLRLPIPEEQAKLDCTI
                     LLTVHTMNYLRSGRHKVAV"
     gene            complement(11293..11784)
                     /locus_tag="VI359_06345"
     CDS             complement(11293..11784)
                     /locus_tag="VI359_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEY6973906.1"
                     /translation="MKLSDKPVRSILFVCTGNVFRSVVAEYATKLQLGLNHPYLVGSA
                     GIEAKLQSVHPWIQQSLREKGVDVSTHVQRKLTHELLKRTDLVVAMSHDHQTFINQEF
                     GIDAPLFKQICYGRDEPILDLHEVHPNWEQDLNQARTYVSSVIDMIWGDIPSFLSQFP
                     RIH"
     gene            11853..12725
                     /gene="ttcA"
                     /locus_tag="VI359_06350"
     CDS             11853..12725
                     /gene="ttcA"
                     /locus_tag="VI359_06350"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY6973907.1"
                     /translation="MQISTNQMPLLPKLDEATEQLQTRLLRLVGQTIAEYGLIEDGDK
                     IMVCMSGGKDSYGLLDLLLVLQRRAPIRFDLVAVNLDQKQPGFPAHVLPEYLTAAGIP
                     FHIEQRDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRVASELGATKIALGHHRDDILET
                     LLLNLFYTGKLKTMPPKLLSKDGRHIVIRPLSAVREVDLARYAELRSFPIIPCDLCGS
                     QKDLKRKQVKAFLLEWEQRSPGCMDSIFAALSNVAPALLLDKRLFDFDSLHSSQCADG
                     YEDSWLDHDPTLHA"
     gene            complement(12832..>13224)
                     /gene="msrA"
                     /locus_tag="VI359_06355"
     CDS             complement(12832..>13224)
                     /gene="msrA"
                     /locus_tag="VI359_06355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6973908.1"
                     /translation="GRTGHAEAVRLTFDPAIVSFRELLEVFFVIHDPTTLNRQGHDVG
                     TQYRSAIFYHSLEQKQAAEEVVETVTKEQIYPNPLVTEIVPAGTWYEAEPYHQEYFAR
                     NPFQGYCTAVVGPKVVKFRKQFASKLKS"
BASE COUNT         2856 a         3933 c         3514 g         2921 t
ORIGIN      
        1 cgtcgtcgcg tcgtcttgcc gttaaagggc gacacgctgc agaactcgca atcgaaatga
       61 cagccacgag tcgtaaaact gcactgcctc gtcatgtagg catcggggct gagcaattca
      121 acgcgcgggc tcttgtagtg gtcaagcgac gggaagctcg tcatcttata catccgctgc
      181 atcttccccg cttcatagtc ggcgaccagt tgaggccaga ggtcttccgc ttccccgcac
      241 acaatcgcgt cggcatactg cagagcttcg tcgggacagt aggtcggatg gacgccgccc
      301 atgacgatcg tccggcctcg cttgcgaaac tccgtggcga tctcatacgc gcgcggagca
      361 tagcaggtca tgatcgagag gccgaccatg tcgcagggat agtcaaagtc aatatcttct
      421 accgcttcat cgaccagatc gacgtcccag ttctccggcg tataggccgc gatggtgggc
      481 aagctgagtt tgggaaacca gaccgccctc cgctccgacg cagttaaatg gtagggattg
      541 ttccgtgggt aggggtcgac caatagcagt cgcttgcccg tcttcgacat agctagctga
      601 ccctgtttgg ttggttggat aagctccatt agtttcccct cctcacaaca aacgagttag
      661 aattccgctc ctgcaccatg tcgcggccat cagcgagtcc ccactggcct taatggaggc
      721 aactccacat ctggaccctg caaagccggc tctgtggcat cctgaggcgt ctcttctgcc
      781 actgccgctt cctgtttttg ctgctcgcct gcatgttgca gcttttgccg ggtgatcgtg
      841 ggagtcaccc ccacatcttc gggtggaatc ggcggtccca caactccaca agccgccaac
      901 agccccgcga tcccgaacag cagtgcagct gtccgacaca tgatttcgcg cgattgtgac
      961 atccaactac tcacgagagc atccgttcaa gctctttaat gcgacgatct acctgcgtac
     1021 gcgccgtgcc tccaatttgg gccttgcggt caattgctgc catgactgtc aagcgagacg
     1081 tcacgctttt ttcaaatcgg ttggagaagg cctgaagctc ctccaacgaa aaatctgtga
     1141 ggtcccgctc ttgatccaag gcggctcgga ccaccctgcc cgtaatcgca tgggcctcgc
     1201 gaaacggcac accccgggtc acaagataat cggcaacttc cgtcgccaat aatccacccc
     1261 ccgagatggc gtgccgaagg gcatctcggt tgatcttcac gcgacgcatc aattcggtca
     1321 agacttgcac cgaagtctgc accgtatcga gcgcatcgaa gagggccggc ttgtcctctt
     1381 gaagatctcg attataactc aaaggcagtg ctttcatcat tgtcaaaaga ttcatcaact
     1441 ggccatagac ccgaccggtc tttccccgta tcaattcagg cacatcgggg ttcttcttct
     1501 gcggcatcat gctgctgccc gtgcaaaagg catccggcag gtcaacgaag tgaaactcct
     1561 gcgacgacca caggatcaat tcttcactca agcgcgacag gtgcatcatg ataatggcca
     1621 agaccgaagc cacttcgatc atgaagtcgc gatcggagac cgcgtccaag ctattctgtg
     1681 tgatggtcgg aaaaccgagc agagacgccg tataccgtcg atcgatcgga tagtttgatc
     1741 cagccaaggc cccggatcct aacggcatca cgttcagccg tgctcgcgca tcccgcaccc
     1801 gttccttgtc gcgctccagc atctccacat aggccaacaa gtggtgagcc accagcaccg
     1861 gctgggcccg ttggagatgg gtataacccg gcatggccac ggttcggttt gccttggcct
     1921 tggccaatac gacacgttga aactcgttca actgaccggc gaggtgatcc agctcttctc
     1981 gcagatacag ccgaatatcc aaggccactt ggtcgttgcg gcttcgtccc gtgtggagtt
     2041 ttcctcccaa atggccgatc agttcggtca gtcggcgttc aatcgccata tgaatatctt
     2101 cgtcggagga aagaaagcga aatcgtcccc gatccagctc ggctttcact gattcgagtc
     2161 cgcgaatgat ggtgcgcgtt tcccccctgg ttagaacgcg ggctttctcc aaagtcttgc
     2221 agtgcgcgac actgccttga acatcttgcg catagagccg ccggtcgatt ccgactgaca
     2281 ccgaaaacgc ttcgacaagc cgatgggtct tggctcgaaa tcttccgccc caggccttgt
     2341 cggcgaccgc gcgtcgaccc gtgcgtccct tccgttgcgc cataaccgtt taggacgacg
     2401 cctttcgtcg ctgagcccga atcgccaatc gaagcgcgtt caagcgaata aaaccctcgg
     2461 cgtccttctg ccgataaacc tcatcctcct cgaatgtcgc gatgtcgaga cggtagagcg
     2521 agttcttgga tttgcgcccg acaaccgtgc aattcccctt gtacaacttc actcgcaccg
     2581 ttcccgtcac gtccttttgc gcctcatcca tcatcttctg aatcacctca cgttccgggc
     2641 tataccaata gccatagtag accagctccg cataccgagg gatcagactg tcacgcaggt
     2701 gcaacacttc gcgatccatc gtgagtgatt cgagcccccg atgcgccgca tgcaatatcg
     2761 tcccgcccgg ggtttcatac accccgcgcg acttcatacc gacataccga ttctcgacca
     2821 aatcgacgcg accgatcccg tgtgcgccac cgagcttgtt gagatgcgcc agaagcgtcg
     2881 ccgcgctcat ctttttgccg tcgacggcaa ccggatcgcc ggcgtcatac tcgatctcga
     2941 tttcttgcgg cttatccggc gctcgctcgg gggacaccgt catttggaag atttcctccg
     3001 gaggcgcttc ccacgggtct tccagaattc caccctcata gctcacatga aacagattca
     3061 gatccatgct gtagggtttc gccttcgtcg ccgtcacggg aatgccgtgt cgctcggcat
     3121 attcgatcaa ttctcgtcgc gccttgaatt cccattcccg ccaaggcgcg atgatcttga
     3181 tcttcggctc caatgccata taggtcagct cgaatcgcac ttggtcgttt cccttgccgg
     3241 tggcgccgtg cgacaccgct tcggcccctt ccttcttggc aatctcgatc tgccgccgcg
     3301 cgatcaacgg acgcgcgatg gacgtcccga gcagataact gccttcatag accgcgttcg
     3361 cgcgcaacat cggaaacacg tggtctttga cgaacgtctc gcgcaggtct tccacataga
     3421 cgctctttac gccgagtttc tgggctttcg tcttcacggc cttgagatct tcgccttgtc
     3481 ccaaatcagc gcaaaacgcc acgacctcac actcataggt ttcctgaagc cattttaaga
     3541 tcacggacgt atcgaggccg ccggaatagg ccagcaccac tttcttaatc gtgcgcttca
     3601 taacgtcttc tgtctcccct ttccgagcag cgtcaccaaa atggctttct gcatgtgcag
     3661 ccggttctcc gcctggtcga tgatcaccga ctgcggaccg tcaagcacct ccgcggtaat
     3721 ttcttctccc cgatgagccg gcaaacaatg catcacgatc gcatcgggct tggcacgatg
     3781 cagcagacgg ctgttcactt gatagggggc caagactttc aggcggcggg cctgttcccg
     3841 ttcacgcccc atgctgatcc acacatcggc ataaatcacg tcggcctctt tcactgcgac
     3901 atggggatcc tgcccgactt cgatcacggc accggtgttc gccgcttcga tccgcgccac
     3961 gtcgaccacg tgttgatccg gttgatatcc gatcggacag ccgagcgcga ttgtcatgcc
     4021 catcttcgca gtcgcttcga tcaaggaatt ggcgacgttg ttcccgtctc cgatatacgc
     4081 gatcttgacg ccctgtaacc gccccttctt ttcccgaatc gttaataggt ccgagagggc
     4141 ctggcaggga tgactgagat ccgtcaggcc gttgatcaca ggcatcgtcg cttccgccgc
     4201 ccattcttcc acggtcgcat ggtcgtacgt gcgaatgacg atcccgtcga gataccgcga
     4261 caacactcgc gccgtgtcag caacgctttc gccacgcgaa agttgaatgt cagccatatg
     4321 caataccaga gcatggccac ccaactggtt cattcccgct tcgaacgaca ctctggtccg
     4381 cgtcgaaggt ttctggaaca acaaccctag ggttttgccg ggcagcaagg agtggaggat
     4441 tccccgtcgc tgccggtctt tgagtttcgc cgccagcttg agcaggtgct cgatctcggc
     4501 cttgctcaca gccgcgacgt cgagcaagtc tttcttcatc gtttgaacgg tcttcatcac
     4561 cccactctac gcctcgtgcg cccgcgcctc ggttcccgca tgtttgctaa agatctgcga
     4621 gagcacctcg atgaggcgat caatctcacg ctgcgtaatg atcagaggcg gcacaaatcg
     4681 caccacgtgc tcgctggtgt tgttaatcag gaggcctcgg gtgaggcagt cgctcaccac
     4741 tgttttcgca tcgatgtcga gctccatccc ctggaggaga ccaaggcctc gtacctcccg
     4801 tacgatgcgg tggcggtcct tgcattcaga caagcccttc gccaaatact cgcccatcct
     4861 gcgggcatga tcgagcacgc ggccctccaa cagcacacga aacacggcga gtgcagcagc
     4921 acacgcgagc gggtttcccc caaacgtcga ggcatgggaa ccaggtccaa agaccttggc
     4981 caccgagtcg atcgcaaggc aggccccgat cggcacaccg ccccccaacc ccttcgccag
     5041 cgtcatgatg tccggctgca ccccgaactg ctcgtaggca aagagcgtcc ccgttcggcc
     5101 catgccggtc tggacctcat cgaatatcaa taagatatcc ttctgggcac acagcgcccg
     5161 gaggccagcg agacaggtac gatccgccac atgaactcca ccttcgcctt gaatcggctc
     5221 gagcatgatt gccgctgtct tgttggtaac cgcctgttcc acctctgcca aactgttgaa
     5281 ggcgacgtag cggaaccccg gaaccagcgg atcatagccc ttctgcacct tctcttgacc
     5341 ggttgccgtc agagtcgcca tcgtgcggcc atggaaggag ttcttcatcg taatgatttc
     5401 gtagcgcccg tccccatact tctcatgaga atagcgccgt gccagcttga tcgccgcttc
     5461 gttggcttcc gccccgctat tgcagaaaaa cactttgtcg gcaaacgaat ggtccaccag
     5521 cacccgcgca agcttcactt gcggttcggt gtagtacaga ttggacgtgt ggatcagttg
     5581 ctgggcctgt cgttggatcg cgtgaacaag atccgggtgc ccatggccta agatattcac
     5641 cgcgatgccg cccacgaagt cgatgtattc ccgcccctcg aggtcgtaga ccttggtccc
     5701 acgcccccgc gtaatggaca gcggctggcg cgcataggtc tgcattaagt agcggctggc
     5761 atcatctttt agttcatccg tcggcatggc aaacgtatcc ccttggaaag taagggaagc
     5821 tagcacagga ccagcttgaa agcaaccgcc aaaggttcag aaagacagag gcagaacgcc
     5881 gctgatgcgc ttgtccgaac aatcagaggg atgtgataag ctctcgcaca tgaaaacctg
     5941 ttcccaatgt cgccaaccaa acgaagccga cgccaactat tgtttccaat gcggcacaac
     6001 gctgacgacg gccaaaaccg aaatagaaac gggttcgccg tcgaccactc ctgttcccat
     6061 gccggatgca gacctctggc accgattcat cggccctcat gctgaccgtt atctggagca
     6121 gttcaaaaaa ttcggccctg aaaacaatcc ccgcttcgcc ctgacatgga attggccggc
     6181 ttttttgttc gtctcgtttc tctggtttct ctaccgcaaa atgtacctct acgccttggt
     6241 gtacgccatc ggaccgatga tctctaccta cttgaccggc gacatgacgg tcggcattgc
     6301 atggagcgtg attgcgggcg ccaccgccaa ctacatctac tactggcatt gccgggaaca
     6361 gatcggcgag atcaaaaaga aagcgtggag tgatccggcc gtccaggacc agagtctcaa
     6421 agaagccggc ggcgtgcagc catacgtgat ctatgtcggc attgccttct acgtcctgtt
     6481 catcatgacc atgatcaagt tgattcagga cggcgagttt gacggcgaac gatcaccggg
     6541 caaaccggcc aggcccgccg caaccggcag tgtgtgacaa caacggattc tgacgccttc
     6601 aagctgccaa ctatcgcttt ctctcagcgg gatgtgcaaa atctgtctcg caagccttca
     6661 acaaactcag tttcccacag gatggtcaaa acgcctgttc agcaaggccg cagcgagtga
     6721 ggatgcgagg cgtatttctt ttcgtacgtc gagggtccga acgacgcgag aacgcagctg
     6781 gcggtcgttt tcaccatcct gttacagggt gctggctgcg ttgccaagag atccacgcct
     6841 gaaaccattc gaagtcttgc tcgtaggcag cgccccctga atcggtggct tcttgtttct
     6901 gatcgagctg ctggtaggtt cgaggccaat tcttctgcca ccaggacttg agttcgtccg
     6961 aaccgtaggc ctggccgttc tggttgagaa tccccgccgc ggtacagatc ggcgcgagga
     7021 gcggccaata gcggtccttc ccgagaccga gactccggaa atgttcgcgg aggagaaaca
     7081 caacccagag ttcggcgctg ttcttcttgt tatgtttgaa gggctgcgtc tgccgaagcg
     7141 gcacgggatc aaaatgtttc acaacggatg tgtagtccgg acctccatag ctgccggcta
     7201 cctccgcaat tccttcgagc atcttcccca attcgacctc cgccaccggt gacgtagccg
     7261 gtccgtgttc gacctcggct tcaacgagag gattcggcgt cgtcagcagt tcgatgagcg
     7321 gtttgagttc gcgcagtcga tggacggaag ccttgaggat ctgctccgac tccagccagt
     7381 agtcaggatc gttcttggtg agccgctcgc ggccgggcgg cggcaagacg gtcggaaccc
     7441 aatagcgcaa gagcacgaag aggatgtagt cgagttgccc accgatctgc gcgagccgtt
     7501 ctgcaagatg acctgattgg atggaggcaa agaatccacg agcccggtcg gcaacttgga
     7561 gcctctcggc aagcgtcacc caccaggcct caaccgcacg ccgggaggga ttggccgaat
     7621 cacccactgt ggtcatggtc ggatgccgta gatggattaa aaagtccgcg catggtactg
     7681 ccgcctcgga tcgaaagcaa ccgcttgact tggacctccg atccagtatc ctggggctgc
     7741 gcaaccacag agaggaggcg tggatggctc ggttgattct cttgcggcat ggtgaatcgc
     7801 aatggaactt ggaaaaccgc ttcaccggtt gggtcgacgt acccctctcg cctcgcggca
     7861 ttcaggaagc caagaatgcc ggagagaagc tccgcagctt cgttttcgat cgggcgttta
     7921 cgtcggtgct cactcgcgcg atcgaaaccc tccggctggt gctcgagacc atcggtcagg
     7981 tgaacatccc tatcgaaaag gacaaagccc tgaatgagcg gatgtacggc gaattgcaag
     8041 ggttgaataa ggccgacacg gcgaagaaat acggcgatga gcaggtgaag atctggcggc
     8101 gcagttacga tatacgtcca ccgggcgggg agagtctcaa agatacggct gatcgagtgt
     8161 tgccctacta tgagagcagg atcaagccct atgctctcaa aggcgagaca atcttgattg
     8221 ccgcccacgg caatagtctc cgggccttgg tcatgcaact ggagcagctg tcgaaggaac
     8281 aagtccttga gttgaatatt ccgacagggg ccccgttgtt gtatgaactc gataagaacg
     8341 gcatggtagt gaatcaccgc tatctctagg aacccacgcc gtcggaaaat ggacgaggtg
     8401 gggccttgca gaccgggtgc gaatcggtgc ggggaatcaa cccctacggc atgaacttct
     8461 catcctctct gtctgagaat gatcggaaca gcgtgacccg ccgctgcgct tccgccgaag
     8521 cccttagggg atgacggcgc ctcgtctcct cacgtcgccc ccctcccgca gagacatccg
     8581 attcactgtc tcccccgtat ggagagagta ggtcatcttc tcaccataag gaggcatcca
     8641 tgaattggac gggtcaattc gtgctgtata gtgtgatgtt gcttgcgact ccggcgctgg
     8701 cagatatggg caagactgca gagccgttga aagctccgcc gggctcgaaa gcagaaaccc
     8761 acatcaacac tggaatcgag cattacgaca tgggccattg ggacgtggca aagaatcatt
     8821 tcctcgaagc cgagaaggcc gatctccaat cagccgaagc gcattataac gtcgccctgg
     8881 tcttggataa gaccggagac cacgtcggcg cgacaacaca tttcaagcgg gcgaaggaat
     8941 tagggaagaa caatgcggac attcagaact cagagatctt gaacaagcac gtaaaaatgt
     9001 gagggcactc tcagcccacg gtacgcggct gggggtcatg cagctttggc tggctgccag
     9061 cgcaggccga ttgagagcag ctcttgtaat agatccttgt acgaatagcc ggcccgctcg
     9121 gccgaatctg cgaagtcttc atcgtgggcg atttggggat ttggattggc ttcaagtatg
     9181 tagaggttac cctgtccgtc catgcggaga tcaactcggg catagccact gaggtcgagg
     9241 gcacggtaca cgcgcttcgc cagatgttga atctcttcga ccttgcattc cggtaagttt
     9301 ttcacttctc ccgatcgaat gccgtatttg tcttgatact tgcggctcca tttcactcgt
     9361 tcagtcgcaa ttcgccgcgc atcgtccggg agcttgtcca tgatcagttc ccacaccgga
     9421 aatgcgtgca gatgaccgtt gcccatcaat cccacgtaga actcccggcc ttcgatatac
     9481 cgctcaatta gagcaccggt cccgacgctg ttatggatga aagcgacccg ttctttgagc
     9541 cgctcatcgt cctggacaat cgaggcttgg gaaatgccca gtgaggcttc ttctgtcact
     9601 gatttgacga tcagtggaaa tgagagccgg gatggccgct tcacggttcg cccttgcggt
     9661 accaccatga attccggaaa tggaattcga tggaacgaaa agagcttctt cgaaagcacc
     9721 ttgtcacggg ccaacattaa tccgcgcgga ttacagcccg tgtagggcac gcgaagcaac
     9781 tcgagatagg acacgacgtt ttggtcgtag accgccaccc cgtcgaattc ctccaggagg
     9841 ttgaacgcga tatggggccg ccactcttcc accgccttac gaatgacacc gaggtcgctt
     9901 ttgacaccca gcggcgtgac ttcgtggccc agcttgcgga gcgtcgacac gacatcgtac
     9961 tccgtcttcc attcagcaag ctccggttcc tgcccggaga gagtatccgg aggaacgaga
    10021 tcctcatgca tcaggacaag gatgcgcagt cgtttcatac tgccaccttg tgccgaccgc
    10081 ttcgaaggta attcattgtg tggaccgtca gcaggatcgt gcaatccagt ttggcctgtt
    10141 cttcaggtat cggcaggcgt aagttcaatt cgcgacagcg ctggatcatg tcctccagca
    10201 cctgatcgat cgtatattga tactcccccg tccacgcggc gaccttgcgg cggacttccc
    10261 ggcgaaagcg cttcagaaag gctgcggccg tcatcttatc cgcatgcgcc gctgcgtccg
    10321 agaagagtcg cttcaaatct ggatcataga aggacggacg ctcgacgccg tagtggctcc
    10381 tcttccggtc gtaatgttca cgtagcgttt tcttaatcgt ctggatcgga tccacctctt
    10441 ccctggtgac cactttggga ggcatatcag ccaggccttt cagaagactg tccatatatt
    10501 gaagcttctt gagcaccggc cagcctttgt accggtcaat ccatgcacta tctggtgtga
    10561 gccacacggc aaacgtttcg gcaaaatctt cgtcaggatg gctttgggca taccagacgt
    10621 ccaaatgccg cacgaagctc ctgctataag gacggggtct gtaatattgt ggatacggtt
    10681 gagacgattt tccaaagacc tgttggcgac ttcgcaaacg acgaatcctg taggcatttt
    10741 cgattgcatg gcccgcttca tgccggagga tgcgcataca ccattccggc gtgccgcctt
    10801 cgacttccaa catctgcgcg agttcgagtt tgacgagtcg aggatgggcg agatagaaag
    10861 gcaccgcgat gccgggcacg ccgtccggcg tgaaccattc gttggagatc cagaaatggg
    10921 gacggaaccg gagttgtttg gcctcaagtt cggcgtacag ttgttcgatc tgtttcgaca
    10981 agaatccgct ttcgaggctc accccaagcc ggcacatagg gagatccaac aacttctcat
    11041 ctggccaaga ggcccattct ggttctgatt gctcagattc cgctcccggg aatgcctgaa
    11101 gtggcgctcg ctgcatgtcc gaagtccttg attgtctgag aacgttcgcc gctttaaaaa
    11161 atatatcacg caatcatctg gagcgatcga atcgcgccag ctaattgaca ccagagtggt
    11221 tgaagtggag aaaaattgtc tctttcgtcc aagaatggac cgtatcaggc ttttggaaaa
    11281 cgcgagcgga tattagtgga tgcgaggaaa ctgggagaga aatgagggaa tgtcacccca
    11341 gatcatatcg atcaccgagg acacatacgt gcgcgcctgg ttgagatcct gttcccagtt
    11401 cggatggact tcgtgcaaat ccaaaatcgg ctcatcccga ccgtagcaga tctgtttaaa
    11461 gagcggcgcg tcgatcccaa actcctgatt gatgaacgtt tgatgatcgt gactcatggc
    11521 gacaaccagg tcggtccgtt ttagcaactc atgggtcaat ttgcgctgca catgagttga
    11581 tacatcgacg cccttctcac gaaggctctg ctggatccag ggatggacgg actggagctt
    11641 ggcttcaata ccagccgatc cgaccaagta ggggtgattg agccccagtt gcaattttgt
    11701 cgcatattcc gcgacgacgc tccggaagac gttcccggta caaacaaaga gaatcgagcg
    11761 caccggtttg tcactgagct tcattccatc ctctaccagg tgtcaatacc aggatcgaca
    11821 gttgcacgat cagccacgta caatactacc cgatgcagat ctcgaccaac cagatgccgt
    11881 tactccccaa actcgatgag gcgaccgagc agctacaaac tcgtctgctt cgtctcgttg
    11941 gccaaaccat tgccgagtat gggctgatcg aggatggcga caagatcatg gtatgcatgt
    12001 cgggcggcaa agacagctat ggtctgctgg atctcctgct cgtgctccaa cggcgggcac
    12061 cgattcgctt cgacctcgtg gcggtcaacc tcgatcaaaa acagccaggc tttcctgccc
    12121 acgtcttgcc cgagtatctc acggcggcgg gaatcccctt ccacattgag caacgagaca
    12181 cttactcgat tgtcaaacgt ctcattccgg aggggcagac gacctgttcg ctctgttcac
    12241 gcctgcgtcg tgggcacctg taccgcgtcg cctcggaact gggtgccacc aagatcgcgc
    12301 tgggtcacca ccgcgatgac atcctggaaa cgctgctcct gaatctattc tacaccggca
    12361 agttgaaaac aatgccgccg aagctcctct cgaaggacgg ccgtcatatc gtcatccggc
    12421 cgctgtcagc cgtacgagaa gtcgatctcg ctcgctatgc cgaattgcgt tccttcccga
    12481 tcattccgtg cgacctctgc ggatcccaaa aggacctcaa gcggaagcag gtgaaggcct
    12541 tcttactcga atgggaacaa cgttcgcccg gatgcatgga cagcatattc gccgctctgt
    12601 cgaacgtagc cccggcactg cttctggaca aacggttatt cgatttcgat tcgctccaca
    12661 gttcacaatg cgccgatgga tacgaagact cctggctgga tcacgatccc actctccatg
    12721 cgtaagtctc acaataactg ctgaacagct cttctctcgc tcgcttcata attggacggg
    12781 ctagacgacc ggtggatatt ttacctgctt aggacattag gaagcgggga gttagctctt
    12841 taatttcgag gcaaactgct tgcggaattt gacgaccttt ggaccaacaa ccgctgtaca
    12901 gtagccttga aagggattcc gagcaaaata ttcctgatgg tagggttcgg cttcgtacca
    12961 ggttccagcc ggcactattt ctgttaccag agggttggga tagatctgtt ccttcgtcac
    13021 ggtttcaacc acttcttccg cggcctgttt ctgctcgaga gaatgataga aaatggcgga
    13081 ccgatattgc gttccgacat cgtgaccttg ccgattgagc gtcgtcggat catgaatcac
    13141 aaaaaagact tcaagcagct cgcgaaagct gacgatggcc ggatcgaagg taagacgcac
    13201 ggcttcggca tgaccggtcc gacc
//
LOCUS       DATDNW010000260         3022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1262471, whole genome shotgun sequence.
ACCESSION   DATDNW010000260 DATDNW010000000
VERSION     DATDNW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262471"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VI359_06360"
     CDS             <1..189
                     /locus_tag="VI359_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973909.1"
                     /translation="WWFVVGAAMALAVIMLQGGIREVMQAQGSVWELKLVELLTTIMG
                     GGLLGGCIALILNRIKKS"
     gene            233..658
                     /locus_tag="VI359_06365"
     CDS             233..658
                     /locus_tag="VI359_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973910.1"
                     /translation="MDVEIGSNLYRNTNGMIAIEGVPQIEVAVKPATGTLLVNFALFD
                     GAGKMIAKLSDSTLMFNERRAYEVSKTAKTLALKQIDSGKIILQMELRAPEVVAITKG
                     EFHTVKGHVLEVTPTEWKIDKVRSSGTKHDMQGGEVAIG"
     gene            complement(671..>3022)
                     /gene="clpB"
                     /locus_tag="VI359_06370"
     CDS             complement(671..>3022)
                     /gene="clpB"
                     /locus_tag="VI359_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY6973911.1"
                     /translation="QLHLATRLSQVLTKAESEQQALKDEYLSVEHVFLAMVQEGGVFK
                     KLGLTRDRVLAALQQVRGHQRVTSQDPEGTYQALEKYGRDLTRLAGQGKLDPVIGRDD
                     EIRRVIQILSRRTKNNPVLIGSPGVGKTAIAEGLAIRIVKGDVPESLKQKRLISLDMG
                     SLVAGAKYRGEFEERLKAVLKEVQASQGQILLFIDELHTVVGAGAAEGAMDAANLLKP
                     MLARGEMHMIGATTLDEYRKHIEKDAALERRFQPVFVDQPSVENTISILRGIKERYEV
                     HHGVRIKDAALVAAAKLSHRYIADRFLPDKAIDLVDEAAARLRTEIESLPAELDEVSR
                     KVLQLEIEREALRKEHDKASQARLATIEKELAEKQRDLQTLKTRWESEKASVGRSRKT
                     REAIEEVKREVERAERAYDLNRVAELRYGELPRLERELNTELQQLNKRQGESRLLKEE
                     VDEEDIAEVISRWTGIPVSRLLEGETEKLLKLEELLHQRVIGQDEGVRVVADAVLRAR
                     SGIKDPNRPIGSFLFLGPTGVGKTELARSLAAILFDDEANMIRIDMSEYMEKHTVARL
                     IGAPPGYVGYEEGGQLTEAVRRRPFSVILFDEIEKAHHDVFNILLQVLDDGRLTDSQG
                     RTVDFKNTVLIMTSNIGSPHILQAQQRKASYEEVRALVLGELREHFRPEFLNRIDEVV
                     VFHVLETDQLIKIVEIQLNRLRTRLAERRITLTISPAALKLLAERGYDPIYGARPLKR
                     LVQQELETPLARQLVKGELKDGDTALVEVKGKELVVVPTVTGT"
BASE COUNT          650 a          852 c          808 g          712 t
ORIGIN      
        1 tggtggtttg tagtgggggc cgcaatggcc ttggccgtga tcatgctgca aggcggcatc
       61 agagaagtaa tgcaggctca ggggtccgtg tgggaactca aattggtcga attgctgacg
      121 acgatcatgg gcggcggcct gctgggggga tgtattgcct tgatcctgaa tcgtatcaag
      181 aaatcctagc cgtcgatcat atcccaacaa cacgagaagg acaaagcgca ctatggatgt
      241 ggaaatcgga tcgaatttat atcgcaacac caacggtatg attgccatcg aaggggtgcc
      301 tcaaatcgag gtggctgtca aacctgcaac cggaacattg ctggtgaact ttgcactttt
      361 cgacggagcc ggaaaaatga tcgccaagtt gtcggacagc acgctcatgt tcaacgagcg
      421 gcgggcgtac gaagtgagca agacagcgaa gacgctcgcc ttgaaacaaa ttgactcggg
      481 gaaaatcatt cttcagatgg aattacgcgc tcctgaggtg gttgccatta ccaaaggcga
      541 gttccatacc gtcaaagggc acgtgctcga agtcacgccg acggagtgga aaattgacaa
      601 ggtacggtca agcgggacaa agcatgatat gcagggtggg gaggtcgcta tcgggtagat
      661 tgatgaatgc ttatgttcct gtcacggtcg gtacgaccac tagctccttg cctttgacct
      721 ccaccaacgc ggtatcccca tccttcaact ctcccttgac cagctgacgc gcgagaggag
      781 tttccaattc ttgctgcacc aaccgtttca ggggccgagc accgtatatc gggtcgtagc
      841 cacgctccgc caatagtttc agggctgcag gactaattgt cagggttatc cgtctctctg
      901 ccaagcgggt acgtagccga ttgagctgaa tctccacgat cttgatcagc tgatcggtct
      961 ccaatacatg gaataccacg acttcgtcga tacgatttaa aaattcaggc ctgaagtgct
     1021 cccggagttc gcccaggaca agagcgcgca cctcctcgta ggaggccttc cgttgttgcg
     1081 cctgaaggat atgcgggctc ccgatgttcg acgtcatgat caacacggtg ttcttgaaat
     1141 ccaccgtacg gccttgagaa tccgtaagcc tgccgtcgtc gagaacctgg agaagaatat
     1201 tgaagacatc atgatgagct ttttcaattt catcaaacaa gatgaccgag aacggacggc
     1261 ggcgcactgc ctccgtcaat tggccgcctt cttcatatcc aacatagcct ggaggcgctc
     1321 cgatgagacg ggccactgtg tgcttttcca tgtactcgga catgtcgatc cgaatcatgt
     1381 ttgcttcatc gtcaaataga attgcggcca aggaacgcgc caattccgtc ttgcccaccc
     1441 cggtgggtcc aaggaataag aacgagccaa tcgggcgatt cggatccttg atgcccgatc
     1501 gggcgcgcaa cacggcgtcc gccacaacgc gaaccccctc atcctgccca atgacacgtt
     1561 gatggaggag ctcttccagc ttgagcagct tctccgtttc accttcaagg agtcgcgaga
     1621 caggaatccc ggtccaccgg ctgatgacct cggcgatatc ctcctcatcg acttcttctt
     1681 tcagcaagcg cgattcaccc tgcctcttgt tgagttgttg gagttcggtg ttgagctccc
     1741 gctccaaacg agggagttcc ccataacgaa gctccgcgac acgattcaag tcgtaggcgc
     1801 gttcggctcg ttccacctct cgcttcacct cttcgatggc ttctcgtgtt tttcgtgagc
     1861 gacccacgga ggctttttca gactcccaac gggtcttcag cgtctgcagg tcacgctgtt
     1921 tctccgccaa ttccttctct atggtcgcca atcgtgcttg actcgcctta tcgtgctctt
     1981 tgcggagggc ctctcgttcg atttctagtt gaagcacttt tcgggacact tcgtcgagtt
     2041 cagccggcaa actttcgatt tcagtgcgca gcctggcggc ggcctcgtcg acgagatcga
     2101 tggccttatc cggaagaaac cggtcggcga tatagcgatg tgagagtttg gcggcggcca
     2161 cgagcgccgc gtctttgatg cggacgccgt gatggacttc ataccgttcc ttgatgcccc
     2221 gcaagatcga aatggtattt tccacagagg gttgatcgac gaataccggc tgaaagcgcc
     2281 gttcgagagc tgcatctttc tcaatatgct ttcgatattc atccagggtt gtggccccaa
     2341 tcatgtgcat ttcaccacgc gccagcatcg gcttgagcaa attggcggca tccatggccc
     2401 cttcagccgc cccggctccc acaacggtgt gtaattcgtc gataaaaaga agaatctgtc
     2461 cttgagaggc ttgcacttct ttgaggaccg ccttgagacg ctcttcgaat tcacctcggt
     2521 atttggcgcc ggcgacgagc gatcccatgt ccagactgat caggcgcttt tgcttcagac
     2581 tctccggcac gtctcccttt acaatgcgaa tcgccagtcc ttccgcaatc gccgtcttcc
     2641 ccacaccggg tgaaccgatc aagacgggat tgttcttggt tcgacgagaa agaatttgga
     2701 tgacccgacg aatttcgtcg tcgcgcccga tgaccggatc gagtttcccc tggccagcca
     2761 ggcgagtcag atcacgaccg tacttctcaa gtgcctgata cgttccctcg gggtcctggc
     2821 tggtgactcg ctgatgccca cggacctgtt gcaaggccgc aagcacgcga tcacgcgtca
     2881 aacccagttt cttgaagacc cccccctcct gaaccatcgc gaggaagaca tgctcgacgc
     2941 tcaaatattc gtccttgagg gcctgttgct cagattcggc tttggtcagg acctgactca
     3001 gtctggttgc gaggtggagt tg
//
LOCUS       DATDNW010000261         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_916965, whole genome shotgun sequence.
ACCESSION   DATDNW010000261 DATDNW010000000
VERSION     DATDNW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916965"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VI359_06375"
     CDS             <1..967
                     /locus_tag="VI359_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6973912.1"
                     /translation="LAREDKRIVAITAAMCEGTGLNIFEKEFPERLYDVGIAEQHAVT
                     FAAGLATQGLRPVVAMYATFLQRAYDQVVHDVATQNLPVTLCIDRGGLVAEDGTTHHG
                     AFDYAYLRHVPNMVVMAPKDENELQHMLKTALQYDGPASVRYPRGVSLGVEMDSRMDV
                     LPIGKGEVLREGTDVAIIAIGVPVWQAVKAAERLSQDGVSTAVINARFVKPLDRELII
                     DAAKRVRYVVTVEEGCKMGGFGSAVLETLSDAGITDVTTKLIGLPDWYIEQGPQDLLR
                     ERYGLTAEGIFQSVKELIGKSPATRDRFSGASIAEHLPHGDEQGS"
     gene            983..2170
                     /gene="ispG"
                     /locus_tag="VI359_06380"
                     /gene_synonym="gcpE"
     CDS             983..2170
                     /gene="ispG"
                     /locus_tag="VI359_06380"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY6973913.1"
                     /translation="MHITRRKTRQIKVGSVKIGGDAPVSVQSMCSTDTRDVAATVEQI
                     RQLEAAGCEVIRVAVPDVEAANALTKIKAAMTVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVEEVIKAVNDHGIPIRVGVNGGSLERPLLDKYGWPSPEALAESALNAV
                     HALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQSDYPVHIGITEAGTAMTGAVKSAMG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMDELMDTLIQEVEILAKEKEAEGNSETTPATEAHSEWEPLVPHTDQPSTLGKEIPVL
                     PNR"
     gene            2255..2329
                     /locus_tag="VI359_06385"
     tRNA            2255..2329
                     /locus_tag="VI359_06385"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2287..2289,aa:Cys,seq:gca)
     gene            2535..3551
                     /gene="hpnH"
                     /locus_tag="VI359_06390"
     CDS             2535..3551
                     /gene="hpnH"
                     /locus_tag="VI359_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY6973914.1"
                     /translation="MAVPISQMFTVSKYVVTQKLKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPEQCWAAAEECGAPIVSIPGGEPLIHPEIATIVQGLVDQKRY
                     VYLCTNAILLERKLDEYKPSKYLTFSVHMDGLRAEHDLAVCRDGVYDVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRQFFDEMMDLGVEGMMISPGYSYQKAPDKQHFLKRTRT
                     RELFSRILSSPKRGWRFNQSPLFLDFLMGRREYQCTPWGNPTYNIFGWQKPCYLLQEG
                     YTKTFRELMELTEWDHYGTGRNEKCADCMVHCGYEASAVEDTFGTISGFGRTVKLTLL
                     PTSR"
     gene            3640..4035
                     /locus_tag="VI359_06395"
     CDS             3640..4035
                     /locus_tag="VI359_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973915.1"
                     /translation="MTDTKNHSSGGQGGALEGRVFRPASFDEVAEAVELAFDYRGDVT
                     LSLKSGDSLCGYIFNRRLGGSESSLELFLADSPHPTLVRYDEIAAIAFTGEDTASGKS
                     WEVWMAKKESERRAESERVATEAKARGHL"
BASE COUNT          986 a         1123 c         1329 g          914 t
ORIGIN      
        1 gttggctcgc gaggataaac gaatcgtcgc gatcacggca gcgatgtgcg aagggaccgg
       61 cctcaacatc ttcgagaagg aatttccgga gcgattgtac gacgtcggca ttgccgaaca
      121 gcacgcggtc acgtttgccg caggtcttgc gacccaaggt ttacgaccgg tggtcgcgat
      181 gtatgcgacg ttcttgcaac gggcgtacga tcaagtggta catgacgtcg ccacgcaaaa
      241 tctgccggtc acgttgtgca tcgatcgcgg aggactggtg gcggaagacg ggacgacgca
      301 ccacggcgcc ttcgattacg cgtatctgcg gcatgtgccg aacatggtgg tcatggcgcc
      361 gaaagacgag aacgaattac aacacatgct caaaaccgct ctccagtacg atgggccggc
      421 ttcggtccga tatccgcgcg gagtcagcct cggtgtcgag atggattcgc ggatggatgt
      481 gcttccgatc ggaaaaggcg aggtgctgcg tgagggaacg gacgtcgcaa tcatcgcgat
      541 cggtgtgccg gtctggcaag cggtgaaagc cgccgaacgg ttgagccaag acggcgtgtc
      601 gacggcggtc atcaatgcgc gtttcgtgaa accgctggat cgcgagttga tcatcgacgc
      661 ggccaaacgg gtccgctatg tcgtgacggt cgaagagggt tgtaagatgg gcgggttcgg
      721 gtccgctgtc ctcgagaccc tgtcggacgc aggcatcacg gatgtgacga cgaaactcat
      781 cggattaccg gattggtata tcgaacaggg gccacaggat ctgttgcgcg agcggtacgg
      841 tttgacggcc gagggaatct ttcagagcgt gaaggaactc atcggcaagt cgccggcgac
      901 aagagaccgg ttcagtggcg cttcaatcgc agaacatctt cctcacggag acgaacaggg
      961 cagctagtac gggtaggtcg gtatgcacat taccagacgg aaaacgcggc agatcaaggt
     1021 cggctcagtg aaaatcggag gagatgctcc ggtgtccgtc caatccatgt gttccacgga
     1081 cacgcgggac gttgcggcga cggtcgagca aattcgccag ctcgaagcgg ccgggtgcga
     1141 agtgattcgc gtcgctgtgc cggatgtcga agcggcgaat gccctgacca agatcaaagc
     1201 cgccatgacc gtaccgttga ttgcggatat tcatttcgat caccggctgg cgctgaaagc
     1261 ggctgaagtc gtcgactgtg tccggatcaa tcccggcaat atcggggcat ggtggaaggt
     1321 cgaagaagtg atcaaggccg tcaacgatca cggcattccg attcgagtgg gagtcaatgg
     1381 cggctcgctc gagcgaccgc tgttggataa gtacggatgg ccctcgcctg aagcattggc
     1441 ggaatcggcc ttaaacgccg ttcacgcact cgaggacgtt ggcttcacca atatgaaggt
     1501 gtcgctgaaa gcgtccgacg ttcatcacgc gatcgacgcg tattggctct ttgctcatca
     1561 atccgattat ccggtgcaca tcggcattac cgaggctggg actgccatga ccggagccgt
     1621 caagtcggcg atggggcttg gctacttgct ctcgcaaggg atcggagaca cgttgcgggt
     1681 gtcattggcg gcggatccag tcgaagaagt gaaagtcgga tttgaaattc tgaagtcgct
     1741 ggaactcaga catcgaggga tcaatgtcat cgcctgtcca acttgcgggc gggtcgagat
     1801 cgacgtcgtt cggatggcca acgaattgga gaagaagctt ggacatatca agacgccgct
     1861 caatgtgtcg gtgttgggtt gtgtggtcaa cgggatcggt gaagggaaag aagccgacat
     1921 cggcattgct ggtggagaag gaaaaggaat tctcttcaag aagggcaagc tcgttcgcaa
     1981 ggtgccgatg gatgaactga tggataccct gattcaggaa gtcgagattc ttgcaaaaga
     2041 aaaagaagcc gaaggaaaca gcgagactac gccagccact gaggcccatt ccgagtggga
     2101 accgcttgtt cctcataccg accagccgtc gactctcggg aaagaaattc ctgtccttcc
     2161 caaccgctag ccttctcgcc gattacgcat ccctgcgcac cgcggattaa gttgtgttac
     2221 tggaagagcc cccgctataa tgccgccctc gcgaggcgcc gtacccaagt ggtaagggag
     2281 cagtctgcaa aactgcgatg cagcggttcg aacccgctcg gcgcctccaa atcacgcttc
     2341 gtgcgtgctg acggcattcc aacgcgggat cgtcgtgatt ttcgtcgtat tttgccggca
     2401 gtttgaggtg cccccctcgt aaattgacga gtaattcgtg ggatgttaga atccaactga
     2461 aactgaaggg gtcttctagc ccagctgccg tttcgacgcc gttcacaaca cagactaagg
     2521 acaaggagag cggaatggcc gttccgattt ctcagatgtt tacggtttca aagtacgtgg
     2581 tcacccagaa gctcaagggg gtgaaacggt acccgttagt gctgatgctt gaaccgttgt
     2641 tccggtgcaa tctggcctgt gccggttgtg ggaaaatcca ataccctgat cacgtgctcg
     2701 acaagcgact gacaccggaa cagtgttggg ctgcggcgga agagtgcggg gcgccgattg
     2761 tgagtattcc gggcggcgag ccgttgatac atccggagat tgccacgatc gttcaagggc
     2821 tcgtcgatca aaagcgatat gtctatctct gtacgaacgc catcctcttg gagcgcaagc
     2881 tcgacgaata taagccctca aaatacctca cgttcagcgt ccatatggac gggcttcgtg
     2941 ccgagcacga tctcgctgtg tgccgtgacg gggtctacga cgtggcggta aaagcgatca
     3001 aggccgcgct caagcgcggc caccgcgtga cgacgaatac gaccttgttt gacgatgcca
     3061 atccggagcg cgtacgacaa ttcttcgacg aaatgatgga cctcggcgtg gaaggcatga
     3121 tgatttctcc ggggtatagc taccaaaagg cgccggataa gcagcatttc ctgaagcgca
     3181 cgagaacgcg ggaattgttc tcgcgaattc tcagcagtcc caagcgaggc tggcggttca
     3241 atcagtctcc gctgtttttg gacttcttaa tgggccggcg tgagtaccaa tgcacgccgt
     3301 ggggaaatcc gacctataac attttcggat ggcaaaaacc ctgttatctg ctgcaagaag
     3361 gctatacgaa aacgtttcgg gaactcatgg aattaacgga gtgggatcat tacggcacag
     3421 gacggaatga aaaatgcgct gattgcatgg tgcattgcgg gtatgaagcg tccgccgtgg
     3481 aggatacgtt tgggaccatc tccgggttcg gtcggaccgt caaattgacc ttgcttccca
     3541 cgtctcgata gtgcctcgcg tgaagccccg atcatctttc gtcatcagca acagagtttt
     3601 gagcttcacg agataggctt cacaaacacc acgcgtctca tgaccgatac gaaaaaccac
     3661 agtagcggtg gtcagggtgg agcccttgaa ggccgagtgt tcaggccggc ttcgtttgat
     3721 gaagttgccg aggccgttga gctcgcattc gactatcggg gtgatgtgac gctatcactg
     3781 aagtccggcg attcactgtg tggctacatc ttcaatcgcc gcttgggcgg atcagaatcc
     3841 tctctcgaat tgtttcttgc cgacagtccg catccgacgc tcgttcgcta tgatgagatt
     3901 gccgcgatcg cttttaccgg tgaagacacg gcctcaggca agtcatggga agtgtggatg
     3961 gcaaaaaaag aatcagagcg acgggccgaa tctgagcgcg tagctaccga ggcgaaagcc
     4021 cgagggcacc tctaggtctt tccctgccgg ttgcacatcc ggtccaccca tattccaccc
     4081 gtttcccaag ccatttcggc ggaattgcgg ccaggccgtc tatccgaatg aagtcaggcc
     4141 tcgatggcac agctatttcc aagtccttgg tcggaaaggc cccatttttt caggaatttg
     4201 gcacctgctc tcgttgacaa acaggaggcc ccgtgctaaa agggtcggcc tcaaaatcgg
     4261 gggagtctgc tcaggcaggg tgttctgttg tgtgaggcag atgttgggaa ctcaccggca
     4321 gaaaagcggc aatgtcgcgg acgtgaggcg ta
//
LOCUS       DATDNW010000262         4603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1195140, whole genome shotgun sequence.
ACCESSION   DATDNW010000262 DATDNW010000000
VERSION     DATDNW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195140"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2753)
                     /gene="hrpB"
                     /locus_tag="VI359_06400"
     CDS             complement(219..2753)
                     /gene="hrpB"
                     /locus_tag="VI359_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEY6973916.1"
                     /translation="MNPTMARLPIEDALPTVLDVLTHENNALLTAPPGAGKTTRVPLA
                     LLDAAWLDGRKILLLEPRRLAARAAAYRMASTLNEPLGATVGYRMRLDTKVSSKTRIE
                     VVTEGILTRLLQHDPSLNAYGVVLFDEFHERSLQADAGLALCLEAQRLFRPDLRLLVM
                     SATLDVVSVADLLHKAPIISSEGRLFPVETRYLDRSLSGPADVAIVRAVRQSLARDDG
                     SLLVFLPGMAEIRRVERKLLDADLGPTVVIAPLHGGLPQEAQDRAIEPAQPGTRKIVL
                     ATSIAETSLTIEGVRVVIDAGLLRLPRFDPRTGLTRLETIRVTQDSAEQRRGRAGRLG
                     PGVCYRLWTAAEHQSLAQRRPPEMLDADLAPLLLDLALWGTTNPSELSWLTPPPLSAV
                     NQARELLLRLGALDAAGHITPHGKQMAALPVHPRLSHMLLKAIPLQLAGHACEIAALL
                     SERDIVRRAAGWQNADLRFRLDILHGQPGHHDGATVDRVACQRVKQTTNLWRQQLSHL
                     LSAQQPDKKDRPNDAGKLLALAYPDRIAQRQRGGDARYLLTNGRGALFPNPDPLGSED
                     YLVIADLDGGQQWARIDLAAPLSLADIEALHADAIDQVETVAWDEQTHAVRAMKQRRL
                     GALVLSEQALGKPDPSLISKALLQGIRQRGLNSLAWTPELRQWRARVHLLRRTEGIDS
                     NWPDLSDETLLRTLEEWLGPYLDGVTTLERVQRIDLAAPLHALLAWDQQRQLERLAPT
                     HLTVPSGSNVRLDYETDDLPVLAVRLQEMFGCRDTPRVVDGKVPVMLHLLSPAHRPVQ
                     VTRDLASFWAHAYHEVKKELRGRYPKHHWPDDPLAALPTAKAKRRH"
     gene            2808..3008
                     /locus_tag="VI359_06405"
     CDS             2808..3008
                     /locus_tag="VI359_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973917.1"
                     /translation="MPTYQWRKTKPTEAGWYWFRGPAHEADAFIVQVDEVGQFQWPDG
                     GYQEVKLTNGEWAGPIELPEEE"
     gene            complement(3066..3305)
                     /locus_tag="VI359_06410"
     CDS             complement(3066..3305)
                     /locus_tag="VI359_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973918.1"
                     /translation="MVSQSPVTHRILGEVKRMHGCDLDTLANSLPDLSWNQVFLEIDR
                     LSRQGEVLVTFGTGGRYMIRLPAPKAASSGHHTRS"
     gene            complement(3348..3815)
                     /locus_tag="VI359_06415"
     CDS             complement(3348..3815)
                     /locus_tag="VI359_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973919.1"
                     /translation="MNTTRRLLLIPAPTVILVSAFCCVLPSAQILAADHPGLLLAPAA
                     PTERPSSPPNWADDLDHRLSTYLKKKYPSYDFTPYTEELDRIRGAVSRRDRWTIKHEM
                     GVFLKMLSNRAYGLGDDAAEELMDLAQQTMPVDEFAIVYPGSMAEAHILRIGA"
     gene            4268..>4603
                     /locus_tag="VI359_06420"
     CDS             4268..>4603
                     /locus_tag="VI359_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY6973920.1"
                     /translation="MSEHKATVEWNRGIEPFSYETYNRDHVVMFDGGVRVPASAAPAY
                     RGNPGHVDPESALVAALSSCHMLTFLALAAKKQFVVDGYRDQAVGFLEKNAKGKLAVT
                     RVILRPKVTF"
BASE COUNT          998 a         1290 c         1388 g          927 t
ORIGIN      
        1 tggcctgcgt tgcagcttca tcctggtcgt caaacggaat gtcgtaggtg ggaggaggca
       61 gcgtgttgag gggatctgcc agcgcctcgt gagtcggctc ggacaagagg cacaacagag
      121 caacggcaag ataggggcgc atcatgcatt accatacggg cctcagaggt tgcaggtcta
      181 ccctacgaaa gggagggacg ttcctctcca tgcaagaact aatgtcgtcg tttggccttc
      241 gccgtgggca gtgcagcgag gggatcgtcg ggccagtggt gcttggggta acggccgcgc
      301 agttctttct tgacctcgtg gtaggcatga gcccaaaaac tggcgaggtc tctcgtaact
      361 tggacaggcc tgtgagcggg cgagagcaag tgcagcatta ccggaacctt accatctacc
      421 acgcgcggcg tgtccctgca gccgaacatt tcctgcagtc gcaccgccag cacagggaga
      481 tcgtccgtct catagtcgag acgaacgttg gacccgcttg gtacggtcag atgcgtggga
      541 gccaaccgct ccaactgccg ttgctggtcc caggcgagca aggcatgtaa cggcgccgcg
      601 aggtcgattc gctgtactct ttccagtgtg gtaacaccgt cgagataggg gcctagccac
      661 tcctccagcg tacgcagcag cgtctcgtcg gatagatcag gccagttcga atcgatcccc
      721 tccgtccgtc gcagcaagtg cacacgcgcg cgccactgac gaagctccgg cgtccaggcc
      781 aacgaattga gcccccgttg acgaatccct tgtagcaagg ccttggagat taaagaggga
      841 tcgggtttcc ccaacgcctg ctccgatagc accaatgcac ccaatcgacg ttgtttcatt
      901 gcacgaacgg cgtgcgtctg ctcatcccac gccaccgtct caacctgatc gatcgcatcg
      961 gcatgcaggg cctcaatatc ggccaatgac agcggggctg ccagatcaat gcgggcccac
     1021 tgctggcctc catccagatc ggcgatcacc agatagtctt ccgagccaag cgggtcggga
     1081 ttgggaaaga gcgcgccgcg gccattagtc aaaaggtagc gcgcatcgcc tccgcgctgt
     1141 cgttgagcga ttcgatctgg ataggctagg gcaagtaact tgccagcgtc attgggacgg
     1201 tcttttttgt caggctgctg agccgacagc aaatgcgaaa gctgttgtcg ccacaagttc
     1261 gtcgtctgtt tcacgcgctg acaggcaacg cgatcgactg tagcaccgtc atgatgacca
     1321 ggctgtccat gcaagatatc caggcggaac cggaggtcgg cattctgcca gccggctgcc
     1381 cgtcgcacga tatcacgttc gctcaataac gccgcaattt cgcaggcatg accggcgagc
     1441 tggagcggaa tggctttgag cagcatgtgg gataagcgcg gatgcacagg caatgcggcc
     1501 atctgtttgc cgtgcggtgt gatgtgcccg gctgcatcca atgctcccag ccgcaataat
     1561 aattctctgg cttgattgac cgcacttagc ggtggcggtg tgagccagga gagttcggaa
     1621 gggttggttg ttccccacag agccaggtca agcaacagtg gcgcgaggtc ggcatcgagc
     1681 atctccggcg gccttctttg ggccaacgat tgatgttcgg cggcagtcca aagccggtag
     1741 cagacgcctg gtccaagccg cccggcccgg ccccgtcgct gctctgcgga gtcctgtgtc
     1801 acgcggatcg tttccagtct ggtcagacca gtccgtggat cgaatcgagg caggcggagc
     1861 aggccggcat cgatcacaac gcgtacgccc tcgatcgtca agctcgtttc ggcgatagac
     1921 gtcgccagca ctatcttgcg cgtgccgggc tgcgctggct cgatcgcgcg atcctgcgcc
     1981 tcttgtggga gaccgccatg gagcggcgcg atcaccacag tcggaccaag gtcggcatcg
     2041 aggagtttcc gttcgactcg ccggatttcc gccattcccg ggagaaagac caggaggctg
     2101 ccgtcgtcac gtgcgaggga ttgccgaacg gctcgcacga tggcgacatc cgcaggacct
     2161 gacagggatc gatcaagata acgggtctcg acaggaaaga gccggccctc gctcgagatt
     2221 atcggcgcct tgtgcaaaag atccgcgact gacaccacat ccaacgtggc cgacatcacg
     2281 aggagccgga gatcggggcg aaagagccgc tgtgcctcga ggcagagtgc aagcccggca
     2341 tccgcctgca agctccgctc gtggaattca tcaaagagca ccacgccata agcattgagt
     2401 gatggatcgt gttgcagcag gcgtgtcaat ataccttctg tgacgacctc gatccgcgtc
     2461 ttcgaactca ctttcgtatc cagccgcatc cgataaccga ccgttgcccc gaggggttca
     2521 ttcagtgtac tggccatgcg ataggctgcc gcacgggcgg cgagtcgccg tggttcgagc
     2581 agaagaatct tcctgccgtc caaccatgct gcatcgagca gggccaacgg gacacgagtc
     2641 gttttccccg cgccgggtgg ggcggttaga agcgcgttgt tttcatgagt aaggacgtcg
     2701 aggaccgtgg gaagcgcatc ttctattggc aatcgcgcca tggtagggtt catagccaca
     2761 ctgcagactg taacagaccg cacatcaaga cgaaaaggat gggatgcatg ccgacctatc
     2821 aatggagaaa gacgaaaccg accgaagccg gttggtattg gttcagggga ccggcgcacg
     2881 aagcagatgc gttcatcgtg caagtcgatg aagtgggaca gtttcaatgg ccggatggag
     2941 gctatcagga agtgaagttg acaaatggtg agtgggcagg gccgattgaa ctaccagaag
     3001 aagaatgaag gaaggaagac acgggatcgt cttctgatat gctctgcgat cgaacactac
     3061 attgatcaag atcgggtgtg atggcccgat gatgccgctt tcggcgccgg cagccggatc
     3121 atgtagcggc caccggtccc aaacgtcacc aggacctctc cctgtcggct cagccgatca
     3181 atctcgagaa agacctgatt ccaagagaga tcggggagac tgttcgccag ggtatcgaga
     3241 tcgcagccgt gcatccgttt cacttcccca agaatgcgat gggtgacggg tgattgggaa
     3301 accatgtgtg tgccctccct ttcctgttgc ggatgatcac tgcgacgtca tgccccaatt
     3361 cgtaatatgt gcgcttcggc catcgatcca ggatagacga tggcaaactc atcgactggc
     3421 atcgtctgtt gggcaaggtc catcaactcc tcagccgcgt cgtctcccag gccataggca
     3481 cgattcgaca acattttcag aaacaccccc atctcatgct tgatggtcca gcgatcccgt
     3541 cggctgacgg cacctcgtat ccgatccaat tcttcggtgt acggtgtaaa gtcgtaggaa
     3601 ggatacttct ttttgagata ggttgaaagt cggtgatcga ggtcatctgc ccagttcgga
     3661 ggtgaggatg gtcgttccgt tggcgcggct ggtgccaaca agagcccagg atggtcagcg
     3721 gctagaatct gagccgacgg gagaacacaa cagaaggcag acaccaggat cactgtcggc
     3781 gctggaatga gaagcaatcg acgggtggtg ttcattggga ccctcgttcc aaaggatccg
     3841 catcaatgag cgagtcgcag atgatacagc ccctggcgta ggagcgtcgg gaactgcgcc
     3901 gggatccaca caccgaatgg tgggtaggag cgtgtggaag agatcgcagt caccgtcact
     3961 ggaacgtcag aacgacatgc tacgcccatc cgcatccttg gtcaactgtc gaggaaggtg
     4021 caagggaagc cggttcgtgg ggtcaatgca ttcggaagca agacgtccac aacacattcg
     4081 cctggaagaa tcggatcgct tcgtctgacg cggtcgtgcg aatattcctc gttcaccagt
     4141 ctttccgttg acactccggt tcttcgcagc tacgctgtcg atcgcacctc tctgcgtgcg
     4201 gacaaggcac gaatgcctcg acagcccatc tgaacaatgg atatctatca atttagaagg
     4261 atgagttatg tccgaacata aggccacggt cgagtggaac aggggaatcg aacccttctc
     4321 gtacgagaca tacaatcgcg atcatgttgt catgtttgac ggaggtgttc gagtgcctgc
     4381 ctcagccgcg cctgcctatc gtgggaatcc cggtcacgtg gatcctgaat cggcgttggt
     4441 agcggccctt tcaagctgtc atatgctgac gtttctggcc cttgccgcca agaaacaatt
     4501 tgtcgtcgat ggctaccgcg atcaggccgt cggatttctg gaaaagaatg caaagggcaa
     4561 gctggcggtc acgcgagtca ttctccgtcc aaaagtgaca ttc
//
LOCUS       DATDNW010000263         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_917557, whole genome shotgun sequence.
ACCESSION   DATDNW010000263 DATDNW010000000
VERSION     DATDNW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917557"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VI359_06425"
     CDS             <1..675
                     /locus_tag="VI359_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY6973921.1"
                     /translation="LFDHLGVEQELTRALSRKVWLKSGGYLVIDHTEAMTVIDVNTGR
                     FVGKRDQEETILRNNLEAAKEVAYQIRLRGIGGIIIVDFIDMEREKNRDKVYHALVDA
                     MASDKARTRISRISDLGLIEISRERVREDLLRSLSEPCHYCEGRGYTKSPTTVAYEIF
                     REIRRIGKGAEQQRIVIGAHPTVAGLLQDEERQGLETLERDCSAKMIVMPDERLHLEQ
                     YDLAVV"
     gene            774..1892
                     /gene="dprA"
                     /locus_tag="VI359_06430"
     CDS             774..1892
                     /gene="dprA"
                     /locus_tag="VI359_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY6973922.1"
                     /translation="MNRTSLESWLWLRAIDGVGDATLLKLVQRWKTPEAVCAATIDEL
                     IEGGCSLQLAAAIHKGPDTVTRRRIDQEWTAIERHRVRMLSVLDRDYPVRLKMIPDPP
                     PLLYVAGTLTDEDGLAVAIVGARKASPSGRLVTEELSHDLAAAGVTIVSGLARGVDAA
                     AHRGALSANGRTIAVLGCGIDRTYPPEHDRLRKQIEEHGAVLSEVPMGTPPHSHHFPR
                     RNRIISGLCLGVIVTEAAMNSGSLITAKLAGEQGREVFAVPGHIKSETSRGSNGLLKD
                     GAGLVEQAQDVLDVLRPQLEPELRAKLHDVVPLKKTIDHLGKDEAIVYDALSYLPSSV
                     DIVIEQTGLQASVVVTALLSLELQQRVRQLPGQRYLKT"
     gene            1981..>4037
                     /gene="topA"
                     /locus_tag="VI359_06435"
     CDS             1981..>4037
                     /gene="topA"
                     /locus_tag="VI359_06435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY6973923.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYTVMASVGHVKDLPTSKLGV
                     DLEHDFKPHYVTIKGKSKILAEIKQKAEAADKVFLAPDPDREGEAIAWHIAQELGGKA
                     KKKKDEKVFRVLFNEITESAVKRALQSPGQIDMKLVNAQQARRVLDRIVGYQGSQLLW
                     NKVRRGLSMGRVQSVAVRLICEREQEREAFRAEEYWSVTALLAGTNPPAFEAKLHSIN
                     GEEASIASSEQAQRVVAAVQGKTFAVESIERKEKKRNPVAPFITSRLQQEAARKLHFS
                     PKKTMTLAQQLYEGVEIGAEGATGLITYMRTDSPRISNDAMADARQVIQDRFGAEYLP
                     ATPNIYKTQKAAQEAHEAIRPTSASRDPESIRQYLEPDVYNLYKLIWNRFIASQMVPA
                     ILDVTRVESSPISTKDKYLFRSTGTVVKFPGHTIVYMEGIDRELFAQKPKGEQEVEED
                     SERQLPVLNESEQLRLVSQEGETVQGLTAKQHFTQPPPRYNEALLIKELEEKGIGRPS
                     TYASIISTIQDRKYVEKIEGRFAPTETGRTVNDFLMKGFPDLINVDFTSQMEEELDEI
                     EEGSKPWVAAIRDFYEPFTRDMEKAKAIPGPKDIVEPPTDIPCEKCGRMMEIKWGRNG
                     KFLACPGYKEDPPCKNTQNFEKLPDGTIKVVPKQELTTDQTCEKCGSAMVVKTGRFGK
                     FIA"
BASE COUNT          985 a         1037 c         1219 g          796 t
ORIGIN      
        1 ctgttcgatc atctcggtgt cgaacaagaa ctcacgcggg ccctcagtcg aaaggtgtgg
       61 ctgaaatcag gcggatacct cgtgattgat cataccgaag ccatgacggt gatcgacgtc
      121 aataccggcc ggtttgtcgg aaagcgcgac caagaagaga ccatccttcg gaacaatctg
      181 gaagcagcca aagaggtggc ctatcagatc aggttgcggg ggatcggggg cattatcatc
      241 gtcgacttca tcgacatgga gcgagaaaag aatcgtgata aggtctacca cgcgttggtc
      301 gacgcgatgg cgtcggacaa agcacggacg aggatctcga gaatatccga tctgggcttg
      361 atcgaaatct cgcgggagcg cgtacgggaa gatctgcttc gttctttgtc ggagccctgc
      421 cattattgcg aagggcgggg gtataccaag tctccgacga cggtggccta cgaaattttc
      481 cgtgaaatcc gtcgaatcgg taagggagcg gagcagcagc ggatcgtgat cggggcgcac
      541 ccgaccgtcg cggggctgct ccaagacgaa gagcggcagg ggctcgagac gctcgagcgc
      601 gactgctcgg ccaaaatgat cgtgatgccg gatgaacggc ttcaccttga acagtacgac
      661 ctggcggtgg tctagttggt ctgctttata atctcgagaa acgtatcgag actctctgcg
      721 gtgtgtctgc ttaactccct cgtagaggca ccctccgtgc gttgacgact cgcatgaacc
      781 gaacgtcatt ggaatcctgg ctctggttac gggccatcga cggggttgga gatgcgacgt
      841 tgctcaaact tgtccagagg tggaagactc ccgaagccgt ctgtgccgct accatcgacg
      901 aattaattga aggaggctgc agtctccaac tggccgccgc cattcacaag gggcccgata
      961 cggtcaccag gcggaggatt gaccaggaat ggacggcgat cgagcgacat cgcgtacgga
     1021 tgctcagtgt cttggatcgc gattatcctg ttcgactgaa gatgatccct gatccgccgc
     1081 ctttgctgta cgttgctggt acgttgactg acgaggatgg actggccgtg gcgatagtcg
     1141 gtgcgcgtaa agcgtcgcct tcaggtcggc tggtgacgga agaattgagc cacgatcttg
     1201 ctgcagcagg cgtcaccatc gtcagcgggt tggcccgtgg tgttgatgca gcggcgcacc
     1261 gaggggcgtt gagtgccaac ggacgcacca tcgccgtcct cgggtgtgga atcgaccgta
     1321 cctatccgcc agagcatgat cgccttcgga aacaaatcga agaacacggc gccgtcctat
     1381 ctgaggttcc aatgggcacg ccgccgcaca gtcatcattt cccacgccgt aaccgtatca
     1441 tcagcgggtt gtgtttgggc gtgatcgtaa ccgaggccgc gatgaacagc gggtcgctga
     1501 tcacggcaaa gctggctggg gagcaggggc gggaagtctt cgccgtgccc ggtcatataa
     1561 aatcagagac gagtcgaggg tcgaatggtt tattgaaaga cggggctgga ttggtcgagc
     1621 aggcacagga tgtgttagat gtattgaggc cgcaattgga accggaactg cgagcgaagt
     1681 tacatgatgt ggtgccgctc aagaaaacca ttgatcactt gggaaaagac gaagcgatcg
     1741 tgtatgatgc cctctcgtat cttccatcct cagtggatat tgtgatcgaa cagactggct
     1801 tgcaggcatc agtagtggtg accgcgctct tatcgcttga actacaacag cgagtgcgtc
     1861 agttgcctgg gcagcgctac ctgaagacct agagtgcacc gcatgaggcc gcccagttgc
     1921 atgtatctgc tatatttgat acgggtaata agctgacgac ttttcagaga cggagtgttc
     1981 atggccaagt cgctcattat tgttgagtct cccaccaagg cacggacgat caccaaatac
     2041 ctcggccgag gttatacggt gatggcatcc gtcgggcacg tcaaggattt accgaccagc
     2101 aagctcgggg tcgatcttga gcatgacttc aaaccgcact acgtgacgat caagggcaaa
     2161 tcgaaaattt tggccgagat caaacagaaa gcggaagcgg cagacaaggt ctttctcgcg
     2221 cccgacccgg accgcgaggg cgaagcgata gcctggcaca tcgcgcaaga attggggggg
     2281 aaagccaaga agaaaaagga cgaaaaggtc tttcgagtgc tcttcaatga gatcactgag
     2341 tcagcagtca agcgcgcgct gcaatctcca ggacaaatcg atatgaagct ggtgaatgca
     2401 cagcaggcgc ggcgagtgct cgaccgaatc gtcggctacc aaggaagtca gttgctgtgg
     2461 aacaaggtgc gacgtggctt atccatgggg cgcgttcaat cagttgccgt tcggttgatc
     2521 tgcgagcggg agcaagagcg tgaagccttc cgcgcggaag agtattggtc cgtgacagcg
     2581 ctcctcgcgg gaacgaatcc gcctgccttc gaagccaaac ttcatagcat caatggtgag
     2641 gaagcctcca ttgcctccag cgaacaggcg caacgggttg tcgcggccgt tcaagggaaa
     2701 acctttgccg tcgagtcgat cgagcgaaag gaaaagaaac gaaatcccgt ggcgccgttt
     2761 atcacgagcc gattgcagca ggaagctgcg cgaaaactcc atttctcgcc gaagaagacg
     2821 atgacgttgg cccagcagct ctatgaaggt gtggaaatcg gtgcagaggg ggcgacgggg
     2881 ctcatcacct atatgagaac cgactcgcct cggatttcta atgatgcgat ggctgatgcg
     2941 cggcaggtca ttcaggaccg gtttggcgca gaatatcttc ctgccacgcc gaatatctac
     3001 aaaacgcaga aggctgccca agaggcccac gaggcgatcc gcccgacgtc cgcttcacgc
     3061 gatccggagt cgatcaggca atatctcgag ccggacgtct acaatctcta caagttgatc
     3121 tggaatcgat tcatcgcctc ccaaatggtc ccggccattc tcgacgtgac acgggtcgaa
     3181 tccagcccga taagcacaaa ggacaagtat ctcttccgat cgaccggaac cgtggtgaaa
     3241 ttccccggtc atacgattgt ctatatggaa ggcatcgatc gcgaactctt tgctcaaaag
     3301 ccgaagggtg aacaggaagt cgaggaggac agcgagcgcc agttgccggt gctcaatgag
     3361 agcgagcagc tgcgattggt gtcgcaggaa ggcgagacgg tccaagggct gacggcgaag
     3421 caacatttta ctcagccgcc gccacgatac aacgaagcat tgctgatcaa agagttggaa
     3481 gaaaaaggca tcggacgccc gtcgacctat gcttcgatca tttcgaccat tcaggatcgc
     3541 aagtatgtcg agaaaattga ggggcggttt gctcccacgg aaactgggcg cacagtgaac
     3601 gactttctta tgaagggatt tcccgacctc attaatgtcg atttcacgtc gcagatggaa
     3661 gaggagttgg acgaaatcga agaaggcagc aaaccgtggg tggcggccat ccgtgatttt
     3721 tacgagccct ttacgcgcga catggaaaaa gccaaggcga ttcccggccc caaggatatc
     3781 gtagagccac cgacggatat cccctgcgaa aaatgcggtc gtatgatgga aatcaagtgg
     3841 ggacgcaacg ggaagtttct cgcctgcccc ggctacaaag aagatccgcc gtgcaagaac
     3901 acgcaaaact tcgaaaaact tccggacggt accatcaaag tggtgccgaa acaggagctc
     3961 acgaccgatc agacctgcga gaaatgcggg agtgccatgg tcgtgaagac cgggcgattc
     4021 gggaagttca tcgcctg
//
LOCUS       DATDNW010000264         3900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_309663, whole genome shotgun sequence.
ACCESSION   DATDNW010000264 DATDNW010000000
VERSION     DATDNW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309663"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2787)
                     /gene="smc"
                     /locus_tag="VI359_06440"
     CDS             complement(<1..2787)
                     /gene="smc"
                     /locus_tag="VI359_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY6973924.1"
                     /translation="MYLKSLEMLGFKSFHEARIEFPDGVTAIVGPNGSGKSNVVDAIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEMRKPLGMAEVSLVISELDRINIDPLSGLSSQLA
                     EYQELMITRRLYRNGDSEYLINKTLCRLKDIRSVLLDTRAGTKGHTVIAQGQIDQILN
                     ASPQDRRELIEETAGIIRYKKQKAEALRKLEATQQNLLRVRDIIAEVKKQLNALERQA
                     RQARSYQTLQQEAKSLEIQLLTHEYRALRSTIRTIETELQTFAQQESGEAAEQARLAA
                     TLEQVRLTVFEANDRIARIREELTTVEQQQAQALTAAEVERNRSGLFEEQLFQGRQEL
                     DRLAEERRRLQSEIEAIEEALVTLERESSEREHAVTALDREMKSLLHQRSTTVAEEER
                     GRVDVLNLAVLVANAEQSLIQLAARMQDIVERDGRLTRERGETETQQTVASDRHQQLQ
                     ASCREAEQLAETLRQQHQRVVQQGDRAAEDLAVIDQQLLRQSEELAAVDSHLRALRGV
                     LQEDMGYGRCGQEEATALKTCAGVHDAVAEWLIIPSGLDRAVDAILGERVRGWFVDGP
                     AAATQAIGFLKGKDLGRGTFIPQQPRWVGRTSSSESWWTTIKDQPGVVGRAVDLIGAE
                     HRREEARNYLFDRVVFVDSLETATRLWEHHSWSAPDGPVLVTSAGEILDAAGVMTGGQ
                     VSATGGLLQRRREVLDLEVQRERLVAAVEDGRQRREHLFAQGLSLKEELRQLADALRD
                     AEMRALSLQKDAVGVEQVLGDLTQRINALTSDATERSREVRQLEQETRSGEAQLAQWM
                     SEKLGQEAGLGQVRARLSQIDHRVQTLQQEMTEAQLIAQGVRTTCEHRQGDLLRLQQA
                     QVETATRVEALTRQLESLAASIEQSRADRLKQETLCQELGQAAVQIKARLVEAQEQQA
                     QDMASARRIESGLDE"
     gene            complement(2955..>3900)
                     /gene="ispH"
                     /locus_tag="VI359_06445"
                     /gene_synonym="lytB"
     CDS             complement(2955..>3900)
                     /gene="ispH"
                     /locus_tag="VI359_06445"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY6973925.1"
                     /translation="NPRGFCAGVDRAIDIVDLSLKKYGAPIFVRHEIVHSRHVVNSLR
                     QKGAVFVEELSEVPEGSVVIFSAHGVAKSVWEEANHRRLHVIDATCPLVIKVHNEVNR
                     DYTQGYDLILIGHAGHPEVIGTLGQIPDKFHLVSSVEDVRNLQVENTTNLSYVTQTTL
                     SVDECRDIVGALHQRFPHIKGPHQEDICYATQNRQNAVKELAKLVDVILVIGSPNSSN
                     SNRLRELGEHCGIASYLIDSASDIDPVWLKNVKAVGITAGASAPEVLVEEVVAYLKTY
                     GTAEVEDLTVIEEDVEFLLPKELITIESSKKSVAIQAG"
BASE COUNT          746 a         1203 c         1020 g          931 t
ORIGIN      
        1 ctcatcaagt ccactctcaa tccgccgagc ggatgccata tcttgcgctt gctgctcctg
       61 ggcctcgacc agtcgtgcct tgatctgaac cgccgcttgc ccaagctctt ggcacaaagt
      121 ttcctgcttt agccgatccg ctcgactttg ttcaatcgag gcggcgagac tctcgagttg
      181 acgggtgagc gcttcgacac gggtagctgt ctcgacttgc gcttgctgaa ggcgcagcag
      241 atcaccttgt cgatgttcac acgtcgtcct gactccttgg gcgatcaact gtgcctctgt
      301 catctcttgt tgcaacgtct gtacgcggtg gtcgatttgg ctgagtcgtg cccgcacctg
      361 tccaagtccg gcctcttgcc cgagcttttc actcatccac tgcgcaagct gggcttcgcc
      421 ggaccgcgtc tcttgctcca gctgccggac ctcgcgcgat cgttcagttg cgtcactcgt
      481 cagtgcattg atgcgctgcg tcaggtcgcc gagtacctgc tcgacaccca ccgcatcttt
      541 ctggagagac aacgctcgca tctcggcatc acgcagtgca tcggccagct gccgcagctc
      601 ttccttcaaa ctcaatccct gagcgaaaag gtgttcccgc cgctgcctgc catcttcaac
      661 cgcagcgacc aacctttcgc gctgcacctc cagatcgagt acctcccggc gtcgttggag
      721 caaccctcct gtggcactga cctgtccgcc cgtcatgaca cccgcggcgt cgagaatttc
      781 tcccgcactc gtcacgagca ccggtccgtc tggtgcagac caagaatggt gttcccaaag
      841 ccgcgtcgct gtttctagtg agtccacaaa aacgacacga tcgaagaggt agtttcgtgc
      901 ctcctcacgg cggtgctccg ccccaatcag gtcgaccgca cgccccacga caccaggttg
      961 atccttgatc gtcgtccacc aggattccga ggaagaagtc cggccgaccc agcgcggctg
     1021 ttgagggatg aacgtacctc gcccaagatc tttacctttc aaaaatccaa tggcttgggt
     1081 ggctgcggca ggcccgtcaa caaaccatcc gcgcactcgc tcgccgagaa tggcgtcgac
     1141 cgcgcggtca aggcctgatg ggatgatcaa ccactccgcc accgcgtcgt gcacaccggc
     1201 gcaggttttg agcgcggtgg cttcttcctg tccacagcgg ccatagccca tgtcttcctg
     1261 caacacaccc cgtagcgccc ggagatgaga gtccaccgca gccaactctt ccgactggcg
     1321 cagcagttgt tggtcgatca ccgccagatc ttcggccgca cgatcaccct gttgcacaac
     1381 ccgctgatgc tgttgccgaa gagtttctgc caattgctcg gcttcgcgac aggacgcttg
     1441 aagctgctga tgccgatcgc ttgcgaccgt ctgttgggtc tcggtttccc ctcgctctct
     1501 tgtcaacctt ccgtcgcgct cgactatgtc ctgcatgcgt gccgctagtt ggatcaagct
     1561 ctgttccgca ttcgcgacca gcacggccag attcaggacg tcgactcggc cccgttcttc
     1621 ttccgccacc gttgtggacc gttgatggag cagtgatttc atctcacggt cgagtgcagt
     1681 cacggcgtgt tctcgctcag aagattcacg ctccaacgtc acaagagctt cctctatcgc
     1741 ctctatctcc gactgcagac ggcgccgttc ttccgcaagc cggtcgagtt cctgtcgccc
     1801 ttggaacagc tgctcctcga acagaccgct ccgattccgc tccacttcgg ccgctgtcag
     1861 agcttgtgcc tgttgctgct cgaccgtggt caactcttcg cggatacggg caatccggtc
     1921 attcgcttcg aacaccgtga gtcgcacttg ctcgagcgtt gccgccagcc gcgcctgctc
     1981 ggctgcttct cccgattcct gctgagcgaa tgtctgcaat tcggtctcaa ttgttcgaat
     2041 ggttgatcgg agcgctcggt attcatgcgt gagcaattgg atttcgagcg attttgcctc
     2101 ctgctggagc gtctgatacg agcgtgcttg ccgcgcttgc cgttctaacg cattcaactg
     2161 cttcttgacc tcggcaatga tatcgcgcac gcgcaataga ttctgctgcg tggcttcgag
     2221 cttgcgcagc gcctctgcct tttgtttctt gtagcgaatg atgccggccg tctcttcgat
     2281 taactcgcga cggtcttgcg gagaggcgtt cagaatctgg tcaatttgtc cctgtgcaat
     2341 gacggtgtgt cctttggtgc ctgcccgtgt atcgagcaac acgctccgga tatctttcaa
     2401 gcggcacagc gtcttgttaa tgaggtattc gctgtcacca ttccggtaaa gacggcgtgt
     2461 aatcatgagt tcctgatatt cagccaattg acttgaaagc ccggatagag gatcgatgtt
     2521 gatccgatcc aattcactga tgaccaatga cacctccgcc atcccgagcg gtttccgcat
     2581 ttcggtcccg ttgaagatga catcttccat cttctcgctg cgaagcgtct tcgtgctctg
     2641 ttccccaagc acccagagaa tggcatcgac gacattgctc tttccgcttc cgttgggtcc
     2701 gacgatagcc gtgaccccgt ccggaaattc aatgcgagcc tcgtggaacg atttaaagcc
     2761 cagcatttcg agcgatttca ggtacatggc gactccttct cgggggtggt atgcccagcc
     2821 tgagcgaaac cagacaaacg ggggttttgt aacacaaggg cttacaggaa atcaaagaaa
     2881 aataccgccg gtagtggggc tcattaaaaa gcccactata ctttgatggg tggatgtgag
     2941 gaaggggaaa cgggctagcc tgcttggata gccactgact tcttcgatga ttcgatcgtg
     3001 atgagttctt tcggaaggag aaattcgacg tcttcttcga tcaccgtgag atcctccact
     3061 tcagccgtgc cataggtttt taagtaggcc acgacttcct caaccaggac ttcaggagcc
     3121 gatgcgccgg cggtaattcc cactgctttc acatttttga gccagaccgg atcgatatcg
     3181 gacgcggagt caatcaagta cgacgcaatt ccacagtgct cccccaactc ccgcaagcga
     3241 ttggaattgg agctattggg ggacccgatg accaggatga catcgaccaa cttggccagt
     3301 tccttgaccg cattttgacg gttctgtgtg gcataacaga tgtcttcctg atgggggccc
     3361 ttgatgtggg gaaaccgttg gtgaagcgcg ccgacgatgt cccggcactc atccacactg
     3421 agcgtcgtct gcgtgacata cgacagattt gtggtgttct cgacctgcaa atttctcacg
     3481 tcttcgaccg aggacacgag gtgaaacttg tccgggatct ggccgagtgt cccgatcacc
     3541 tcgggatggc ccgcatggcc gatgagaatg agatcgtatc cttgggtata atcgcggttc
     3601 acctcattgt ggactttgat aacaaggggg cacgtcgcat cgatgacatg caaccgtcga
     3661 tgattggctt cctcccaaac ggacttcgcc acaccatgcg cgctgaagat caccaccgaa
     3721 ccctctggaa cttcgctcaa ttcttcgacg aatacagccc ccttctgacg gagtgagttc
     3781 acgacatgcc ggctatgcac gatttcgtgc cgcacaaaaa tcggcgcacc atatttcttc
     3841 agcgacagat cgacgatatc gatcgcccga tcgactccgg cgcagaatcc ccgggggttg
//
LOCUS       DATDNW010000265         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_654923, whole genome shotgun sequence.
ACCESSION   DATDNW010000265 DATDNW010000000
VERSION     DATDNW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654923"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /gene="msrA"
                     /locus_tag="VI359_06450"
     CDS             complement(<1..312)
                     /gene="msrA"
                     /locus_tag="VI359_06450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY6973926.1"
                     /translation="MNPSTIARSSPHETATLAGGCFWCLEAVYDQMKGVISVESGYIG
                     GHTSNPTYEAVCSGRTGHAEAVRLTFDPAIVSFRELLEVFFVIHDPTTLNRQGHDVGT
                     QY"
     gene            complement(436..765)
                     /locus_tag="VI359_06455"
     CDS             complement(436..765)
                     /locus_tag="VI359_06455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973927.1"
                     /translation="MERRQHRRVPAHLKSLLLGNSHEVEGITLDLSIGGARIESEIDV
                     FPGKQIQVRLVVPGEEEPLLIDQALVRWVAEDTFGIEFLTVNPEGRDDLEELIDAFEE
                     LEEGGHA"
     gene            complement(802..960)
                     /locus_tag="VI359_06460"
     CDS             complement(802..960)
                     /locus_tag="VI359_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973928.1"
                     /translation="MDSRYAITVKFIVNADSAEEAEELVEAACEKAAAAFSEISYEGI
                     EDIEEEEE"
     gene            complement(1231..1563)
                     /locus_tag="VI359_06465"
     CDS             complement(1231..1563)
                     /locus_tag="VI359_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6973929.1"
                     /translation="MEKRAQPRFTAQFRSTFSGGQREGHGRTLDLSAGGCRIETDMPV
                     VIGAAFECRIFAPNLDWPLRIDEAQVRWVRGNTFGVAFAKLKEEERVKLNRLVKALEA
                     EFNTVAPK"
     gene            complement(1668..2063)
                     /gene="pal"
                     /locus_tag="VI359_06470"
     CDS             complement(1668..2063)
                     /gene="pal"
                     /locus_tag="VI359_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY6973930.1"
                     /translation="MSERQRAELTKEEKAAIEAGLQDVFFGYDQWVISDSGMNALNRD
                     AEWLKTHPGAVMKVEGHCDERGTGEYNFVLGEKRAKAARSYLVEMGVSPKQIAIVSYG
                     KERPFCAEHDEACYQQNRRGHVLLSVKKK"
     gene            complement(2522..2668)
                     /locus_tag="VI359_06475"
     CDS             complement(2522..2668)
                     /locus_tag="VI359_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973931.1"
                     /translation="MNPVILHEVRGGFFLLVGFAMLAFICALAALGTVMRPIRWADRR
                     SAHF"
BASE COUNT          889 a         1060 c          909 g          943 t
ORIGIN      
        1 atattgcgtt ccgacatcgt gaccttgccg attgagcgtc gtcggatcat gaatcacaaa
       61 aaagacttca agcagctcgc gaaagctgac gatggccgga tcgaaggtaa gacgcacggc
      121 ttcggcatga ccggtccgac cactacacac cgcttcatag gtcgggttac tcgtgtgacc
      181 tccgatgtag ccggactcta ccgagataac acccttcatc tggtcgtaga ccgcttccaa
      241 gcaccagaag cagccgccag caagagtcgc cgtttcgtgt ggagatgaac gagctattgt
      301 cgaaggattc accgctacct tgttcgtcat tttcgctgga atgtcggagt acttgcaccc
      361 tattctgtgc gtgagcagga gatgtttaca gaatctcttc cgagtccatt gtgatccagg
      421 cgtcgatgtt ccaattcagg catgcccccc ctcttcgagt tcctcgaagg catcgattaa
      481 ttcctccaga tcgtctcgac cctccggatt gactgtgaga aattcgattc cgaacgtatc
      541 ctccgcaacc caacggacca acgcctgatc aattaacaac ggttcttctt cccctggcac
      601 caccagccgg acttgaattt gttttcccgg aaatacgtca atttcgctct caatcctggc
      661 accaccgatg gacagatcga gagtgatacc ttcaacctca tgggaattcc ccagtagcag
      721 gctcttgagg tgagccggaa ctcgacggtg ttgtcgtctc tccatcaagc ctacggcctc
      781 cctatcgggc cgaagaaggc cctactcttc ttcctcttca atgtcttcta ttccttcata
      841 gctgatttct gagaaggccg cagcagcttt ttcgcacgcg gcttcaacca actcttcagc
      901 ctcttctgcc gaatcggcat tgacgatgaa cttcaccgta attgcatatc gtgaatccac
      961 tgctcctcct ttttgtctat tgcttcaaca caggcccgtg aacggtagac gtgctactgc
     1021 ctcgctcctc ttggctcgaa aactatcttc gatccgacgg acagaatagc tcctttcagg
     1081 cagggtgagg aatacgcctc tcaccacgag aaatcaacct gtgactcgtc cccaatgtgt
     1141 tcagtggtga catcgcaaag ggggtgggga agatctggaa gattatgatc tggagcccta
     1201 tggaaggaat ctatcgaagg gtctgaacat tcacttgggt gcgactgtgt tgaattcagc
     1261 ttcgagcgct ttcaccaagc gatttagttt gactcgttcc tcttccttca atttcgcaaa
     1321 tgcgacacca aaggtgttcc cacgtaccca tcgtacctga gcttcatcga ttcggagtgg
     1381 ccaatcaaga ttgggggcaa agatgcgaca ctcaaacgcc gcaccgatga cgaccggcat
     1441 atcggtttcg atcctgcatc ccccagcaga taggtccaac gtccggccat gtccctcccg
     1501 ttgtcctccg gagaaggtgc ttcgaaactg tgcggtaaac cgtggctgcg cacgcttttc
     1561 catcctgacg ttcctccgtc cacatcacgg aatgacgcga aacaaagaac cccggtgcct
     1621 cgtgtggtta ggtgcaccgg gggttcgtaa tgcctgcggt tacgatctta tttcttcttt
     1681 acggacaata gcacgtgccc gcggcggttc tgctgatagc aggcttcgtc gtgttcggca
     1741 cagaagggcc gctctttccc atacgagacg atggcaattt gctttgggct cactcccatc
     1801 tcgaccaaat agctcctcgc agctttggcc cgcttctcac ccagcacgaa gttgtattcg
     1861 cccgtgcccc gttcatcaca atgtccttcc actttcatca ccgcacctgg atgggtcttc
     1921 aaccattccg catctcgatt caaggcgttc atgccggaat ctgaaatcac ccattgatca
     1981 tagccaaaaa agacatcttg aagccccgct tcaatcgcag ccttttcttc tttagtcagt
     2041 tctgcacgtt gccgttcact cattcctgac gaagacaccg aagagatgcc accgccaggt
     2101 tgcaaccgct cttcagaggg gttttgcgag aagccgctca actctccgct cgatcctccc
     2161 ccagatatcg tgggaagttg cgaattgccg ctttggccat ttgccgattg tccagtctcg
     2221 ctgtccccat ccgaatgcca ccacttggaa ctgcatccct gtccggcaat gagaatcaaa
     2281 cccataactg ctatagcacc tgatctgaat gtttttccgt acatggttcc tctccaattc
     2341 ctttcagtga ataatctgat gcaacaaata tagcaccaga agcgaaaact cgcggcaagg
     2401 agaggcttgg atgtttattg agaaatatta gatagttaga atgatcagtg gctagtgggg
     2461 ggcctggaat gtgggggagg ctttaccaaa actcaagaac aagtgcgatc tgaccagcct
     2521 gctagaaatg ggcggatcgt cgatcagccc atcgaatagg gcgcatgaca gtccctagag
     2581 ccgccaaggc acaaatgaac gcgagcatgg caaaacctac tagcaggaaa aacccacccc
     2641 tgacttcgtg gaggataacc gggttcatgg gatatccctc ctcatggtgc ctgtgtcgac
     2701 tacccgctct gccatccgca ggaacaaacc caccgagcta caggatcgtg cttcgtgagc
     2761 caacgtccac agcaggggca gtgccgtgtc caagccagtc gttctccgtg accaatggac
     2821 ctttcgccaa gggagcacgc cgcctctgac ttaaaggata ccaccatcgt agactccgtc
     2881 atcttctgca cggttgtacc ttctaccttt attacactat cgctggaaga ttgaagagtc
     2941 tatgcagctt tggtggggga gttcacccgt cgaagtgagt aagacagtat tcacaactta
     3001 ttgatattac agcactcacg aaggtctctc acaagatagc aagtaaagta aatggtgcgc
     3061 ccggcaggaa ttgaacctgc gacctgcggg ttcgaaggcc tttagagtgg ttctctataa
     3121 ctccctgatc ttgcgtctgg tcaaccgttt ttattgtgaa gtctgaatgt taggagcatt
     3181 ccatctattc gagttgttcc tattagtttg cgttcttttg gaggactttt agcacaaatt
     3241 tagcacagtg aattggagtg aacgccgaca ggctgacgaa cgaaagttga aaaacagttg
     3301 gtgagtttga cggagcctaa taacatggac cttgccattg ctgctagtcg attagcttca
     3361 aaaggacggt caggaaagca ttgtctcgac gctgggtcat ccagacgggg aaggctctta
     3421 tcggagccac atagacgctg gattcccata gatcacgggg cgctatggca gtgcgcgttg
     3481 gtaattcctc catcagacac aaagaaaacg aattcaccac gcgcatatca ttcgcattgg
     3541 gtctccggat tcaaagttcc tgtcgctcgt cctctgcagt cctatggcaa atccatcgaa
     3601 cacgaaacgc tatacgaagc acagtcgttt ccactgtcct gctccttgca ctgttgattc
     3661 ctgtcctaca catctacgcg gcagagccag caacggtcga acctaggaca ctcaccttcg
     3721 acgcggttaa ctttgtacca cgtgaacccg accgcgtggt gttggaaaat gggatggtgg
     3781 tgttcttatt ggaggatcat g
//
LOCUS       DATDNW010000266         3210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_990987, whole genome shotgun sequence.
ACCESSION   DATDNW010000266 DATDNW010000000
VERSION     DATDNW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990987"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VI359_06480"
     CDS             <1..276
                     /locus_tag="VI359_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973932.1"
                     /translation="QFEEAKTYFSQAMALAPQSGQAHYNYALALNALGDTETARQQFM
                     EAANLAPGDKTIWDSPALSPFGNPQTPKSQKEHPYGTGRPVFGGGPR"
     gene            302..769
                     /gene="bcp"
                     /locus_tag="VI359_06485"
     CDS             302..769
                     /gene="bcp"
                     /locus_tag="VI359_06485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY6973933.1"
                     /translation="MAKELAVGDKAPDISLPDQSGKTVSLKNFKGKQVVLYFYPKDDT
                     PGCTKEACSFRDEEAALKKAGAVVLGVSMDGKASHEKFIAKFKLPFTLLSDEEAAVSK
                     AYGVYKLKNMYGRTYWGLERSTFVIDGTGKLKAIFRKVKVDGHTEEVLDALRA"
     gene            829..1701
                     /locus_tag="VI359_06490"
     CDS             829..1701
                     /locus_tag="VI359_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973934.1"
                     /translation="MRGPRPTIRTWWAVVLLLLSVEGTSVWSAEKVTGTLLVKDALSG
                     VNQPVAIEAKLARKGLLGDAGLGGEPLELVQNGEIVAKAMTGGDGRAVFQFTPKKRGT
                     AMVTVRVAGSPRVGPAEAMANVAVWERRTPILAVEVAALMEEETSSSPVPTLPLGKKA
                     ERHPIPDAADELSKLTQFYYNVIYMVTAGDDRSGGFSTNEMVRAWLKTHKFPSGHILV
                     LPGGSDAVGMQLDELHTAGWKTLKVGIGRSKAFAEAFLQRRLEAVIVPEPTKGEVPRK
                     AKVAKDWKEVRKKL"
     gene            2048..>3210
                     /gene="radA"
                     /locus_tag="VI359_06495"
     CDS             2048..>3210
                     /gene="radA"
                     /locus_tag="VI359_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY6973935.1"
                     /translation="MRAKTSFSCQACGHQAPRWLGRCPDCGGWNTMKEERQAATGKGR
                     PASVKAAPAIATPIADIEIVGEPRRLTRIGEFDRVLGGGVIPGAVILIGGDPGIGKTT
                     LLLQALPRLAGKHEQVLYVSGEESPRQIKMRGQRLGIEHPNLLILAETSLEQILKAVQ
                     EIQPAALVVDSIQTVYTEQITSAPGSISQVQEVAGQLMWLAKRAGVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEMKDSGLEEVSNP
                     SELFLAERSHRSTGSVVVSSLEGSRPILVELQALVSSTSYPMPKRMANGVELNRVSLL
                     LAVMEKRLGLHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREVPIEPSLL"
BASE COUNT          717 a          837 c          983 g          673 t
ORIGIN      
        1 caatttgagg aggccaaaac gtacttttct caagcgatgg cactggctcc tcaatcaggc
       61 caggcccatt ataactatgc gcttgcactc aatgcgctgg gcgacacgga aactgcgagg
      121 cagcagttca tggaagccgc aaatctcgcg ccgggcgata agaccatctg ggattctcct
      181 gccttgtccc cctttggcaa tccacagacg ccgaagtcgc agaaggaaca tccctatggg
      241 actggtaggc cagtgtttgg cggcgggcct cggtgaggat ctcagacgga aggattgagt
      301 aatggcaaaa gaactcgcgg tcggggacaa agcgcccgac atctctttac ccgaccaatc
      361 gggaaagaca gtcagcctga agaatttcaa gggcaagcag gtcgtcctct atttctatcc
      421 gaaagatgac acgcccggct gtaccaaaga agcctgtagc tttcgtgatg aggaggcggc
      481 gttgaagaaa gccggtgcgg tagtgctcgg tgtgagcatg gacgggaaag cctctcacga
      541 gaagtttatt gccaagttca agttgccgtt cacattgttg agtgatgaag aggccgcggt
      601 ctctaaagcc tatggcgtct acaaactcaa gaatatgtat ggccgaacct attggggtct
      661 cgaacgcagc acgtttgtga ttgatggaac aggaaaactg aaggctatct ttcgtaaggt
      721 caaagtggac ggccatacgg aagaagtgtt ggatgctctc agggcgtagc cacgacagtt
      781 tgtttgtcaa accagtcagt tgatttccca ttccatatga ttgcttcggt gcgaggacca
      841 cgaccgacca tccgaacttg gtgggcggtg gtcctgctgt tgctttccgt ggaaggaacg
      901 tctgtctggt cggccgagaa ggtgactggc accttgctcg tcaaggatgc gctgagcggg
      961 gtcaatcaac cggttgctat cgaagcgaaa ctcgctcgaa aaggtctgtt gggcgacgcc
     1021 gggttgggag gagaaccact cgaactcgtg cagaacggag agattgtcgc gaaagcgatg
     1081 accggaggtg acgggcgcgc cgttttccag ttcaccccca aaaaacgtgg cacggcaatg
     1141 gtcaccgtgc gggtagcggg aagtccgaga gtgggccctg cggaagcgat ggcgaacgtc
     1201 gcagtctggg aacgtcggac accgatccta gccgtggaag tcgcggcttt aatggaagag
     1261 gagacgtcgt cctcccctgt gcctacactt cctctgggca agaaggcgga acgtcacccg
     1321 attcccgatg ccgccgacga actgagcaag cttacgcagt tctattacaa cgtcatctat
     1381 atggtcactg caggggacga tcgctcgggt gggttctcaa caaatgagat ggtgcgagca
     1441 tggttgaaaa cacacaagtt tccctctggt catatccttg ttcttccagg tggttctgat
     1501 gcggtgggca tgcagttgga cgaactccac actgccggct ggaaaacgct caaagtcggt
     1561 attggtcgat cgaaagcctt tgcggaggcg ttcctccaac gtcgtctgga ggcggtgatc
     1621 gtgccggaac caaccaaagg cgaagtgccg cgcaaagcca aggtggcgaa agactggaaa
     1681 gaagtaagaa agaagctgtg aaggcagggg gggtgtgacg gctggatgtt cctcctgttc
     1741 ccagaggacg ctgtgaacac gaggacactc tcgggttctc ccatccttgt tatagtactc
     1801 actgtgtgga tcagaggggc tctgcgcgga catggtcgga ggaacggcga cccgcctgga
     1861 ttcttcgatc atcattaaga gggcgatcgg atctgcccct aactgcgtgc gtcgaacgag
     1921 ggccttctga ggccgcgcgt tccgcgagca cgatgggcag ttcgattgcc cttgtcacgc
     1981 ctttccttta gcgatctctt tccattaata tgaggctcag gtcttccttt aattgaggca
     2041 tccccaaatg cgggcaaaga cgagtttttc ctgccaggcc tgcgggcatc aagcgcctcg
     2101 gtggctcggc cgctgtcctg attgcggcgg ctggaatacg atgaaagagg aacggcaggc
     2161 ggcgaccggc aagggccgtc ctgcgtccgt caaagccgcc cctgccatcg ccactcccat
     2221 cgcggacatt gagattgtcg gagagccgag gcggctcacc cgaatcggtg agttcgaccg
     2281 ggtgctcggc ggcggagtca tccccggtgc agtcattttg atcgggggcg atcccggcat
     2341 cggaaagacg acgctgctct tacaagcgct tcctcgattg gcgggcaagc atgagcaagt
     2401 cctctatgtc tccggtgaag agtcaccgcg acagatcaag atgcgcgggc agcggctggg
     2461 gatcgagcat ccaaatctcc tgatcctggc ggaaacctcg ctcgaacaaa tcctcaaagc
     2521 ggtgcaggag attcaaccgg cggctctcgt cgtcgattcg attcaaacag tctacaccga
     2581 gcaaattacg tctgcgccgg gcagcatcag ccaggtacag gaagtagccg gccagctcat
     2641 gtggctggcc aagcgcgccg gtgtgccggt gttcatcatc ggccatgtca cgaaggaagg
     2701 ggcgattgcc gggcctcgat tgctcgaaca tatcgtcgac accgtcttgt actttgaggg
     2761 agataaaggc catagctacc gaattcttcg agccgtcaag aaccgcttcg gatcgaccaa
     2821 cgagatcggt gtattcgaga tgaaggattc cggattggaa gaagtgagca atccctccga
     2881 gttgttcttg gcggaacggt cacatcgcag caccggatcg gtcgtggtat caagcctgga
     2941 agggtcacgg cccattttgg tggagttaca ggcgctggtg tcgtcgacca gttatcccat
     3001 gccgaagcgc atggccaacg gggtggagct taatcgtgtc tcgctgctgc tcgcagtcat
     3061 ggaaaagcga ctgggactac atttgtccgg gcaagatgtc tacgtgaatg tcgtgggcgg
     3121 aatgcatatc gacgagccgg caatcgatct tggaattgtc gccgccgtca catcgagttt
     3181 gcgggaagtc cccatcgagc cgagtttact
//
LOCUS       DATDNW010000267         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_377034, whole genome shotgun sequence.
ACCESSION   DATDNW010000267 DATDNW010000000
VERSION     DATDNW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377034"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VI359_06500"
     CDS             complement(<1..401)
                     /locus_tag="VI359_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY6973936.1"
                     /translation="MSALKLVLWAFLAILSAISLAFVTGLINPHEKVNGLWLVVAAAC
                     IYVLAFRFYGRWISRRVVELNDQRVTPAIRLNDGVNFHPTNKYVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQD"
     gene            586..999
                     /locus_tag="VI359_06505"
     CDS             586..999
                     /locus_tag="VI359_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973937.1"
                     /translation="MKPILFHDIDGVLFGEYDGAFQLRPGVNAWLEWAFEHFEVVWLT
                     SWDQEKIKALLTVTYNAHLLHQFQHANWTNYADKTVWLGEAMTRLGEREVFWIDDEAV
                     PVENIEAIRVNPKGAHELVAVQAQLDQLLAFLHED"
     gene            complement(1244..1864)
                     /locus_tag="VI359_06510"
     CDS             complement(1244..1864)
                     /locus_tag="VI359_06510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6973938.1"
                     /translation="MALWHSIAQQFRQPAGWVGRLAGFFFRINREGIDWTINLLEIQP
                     TDHVLEIGFGPGHGVQQAASLATQGRVAGVDFSHTMLERASQRNAMAIDSGLVELCLG
                     DATSLLYPEKTFHKVFATNVIYFWNDPLVNLQELRRVMKPGGRLALYVIAKENLIKFK
                     VTQTGVYQLYTGEDLVGLLTQAGFHQARFVTKAERHRTGICALAEK"
BASE COUNT          492 a          606 c          597 g          476 t
ORIGIN      
        1 aagtcctgca ccgctccagc cagcacggct ccgatcacga gccagagaaa gccagggaga
       61 aaaccaaact gcgccgccag caccggaccc agcagcggac cagctccagc gatcgcggcg
      121 aagtgatggc cgaagaggac gtatttattg gtgggatgaa aattgactcc atcattcaga
      181 cgaatcgcgg gcgttacccg ttgatcgttc agttccacta cgcggcgcga aatccaacgg
      241 ccgtagaacc gaaatgcgag cacatagatg caggccgccg cgacaaccag ccaaagccca
      301 ttcacttttt cgtggggatt gatgagtcca gtcacgaagg caagagagat ggcactgaga
      361 attgcgagga acgcccacaa gaccagcttt aacgccgaca tgcgcggcat cctatcacac
      421 ggaatgggtt cacttcccgg ggggcacgag taaacgtgag atgccaccca ccgatatgat
      481 tcaaggcaag cagggccgtg tgacgcgccg ccaagccgac aggaggtccc gaggtatact
      541 gttattgaag gctcgccaca acagctccaa caggagggcc agcatatgaa gccgatcctg
      601 tttcacgaca tagatggtgt gttgttcgga gagtacgatg gcgcgtttca gttgcggcct
      661 ggagtgaatg cctggctaga gtgggctttc gagcactttg aagtggtgtg gttgacctcg
      721 tgggatcagg aaaaaatcaa ggccctgttg actgtgacgt acaacgcgca tctgctgcac
      781 cagttccaac atgctaactg gaccaactac gccgacaaga ccgtatggct gggtgaggca
      841 atgacacggc tcggtgagcg agaagtcttc tggattgacg atgaagcggt gccggtcgaa
      901 aatatcgagg cgatccgcgt gaacccgaag ggcgcacacg aattggtcgc cgttcaagcg
      961 cagcttgacc agctccttgc ctttctccac gaagactgag aaaactctgt cagctttgag
     1021 tgaagggtcg ccgccccctt attccccgtc tcggtgtcag atacgagctt aaaatctctt
     1081 cggcgtacaa aatccactca accatgccca ttcacgtttt cggtaaagtg tcgtcgttcc
     1141 tgcggtatgt tagtgaagaa tccgtcggta accattcggg agaagatggg ttgggatgcc
     1201 agtggttcta gagactgtcc ttccaagctt gctcgttatc cgctcatttc tccgcgagcg
     1261 cgcagatccc agtccggtgt cgttcggcct tggtgacgaa tcgcgcctgg tgaaatcctg
     1321 cctgggtcaa aagccccacc aagtcttcgc ccgtgtagag ttgatataca ccggtttgcg
     1381 tcaccttgaa cttgattaga ttctctttcg cgatgacgta caacgccagg cggccgcccg
     1441 gtttcattac acgccggagt tcctgtaggt tcaccagcgg atcgttccag aaatagatga
     1501 cgttcgtcgc gaacactttg tggaaggttt tttctggata gagcagcgag gtggcgtcgc
     1561 cgaggcacag ttcgaccagt cccgaatcga tggccatggc attgcgttga gaggcccgct
     1621 ccagcatcgt gtgagaaaaa tccacaccgg cgactctgcc ctgggtggcc aggcttgccg
     1681 cctgctgaac accatgaccc gggccaaagc cgatttccag cacgtgatcg gtcggttgaa
     1741 tctccagcag attgatggtc cagtcgatcc cctcgcgatt gatgcggaag aagaaacctg
     1801 cgagccgccc aacccatcca gccggttgtc gaaattgttg tgcaatgctg tgccacaatg
     1861 ccatatactg ccccagttat ctacgtaggc cagcactgta tcgtgacgaa atcagccgat
     1921 taactttcca tcttcgtgcc ccttaaggag ggggaaattc ttgctgaatg ctgctttttg
     1981 ctgtcatctt tttgctcggg atttccctcg tcgatgcccg cgctcgttgg ccaccgcctc
     2041 atccatcgaa cggcctctat gaatcaactg gttctgagga gcccatcttt ctggttatac
     2101 ggctggtccc cgcattgcac ttttcctggt caaaactacg gacgaaagga gacccgccat
     2161 gtcgtcgctc a
//
LOCUS       DATDNW010000268         4282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_507321, whole genome shotgun sequence.
ACCESSION   DATDNW010000268 DATDNW010000000
VERSION     DATDNW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507321"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VI359_06515"
     CDS             <1..276
                     /locus_tag="VI359_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HEY6973939.1"
                     /translation="HADALVVTGPVTVNMERALKDVYNQTPDPKIVIALGDCAINCGM
                     FKGSYAVTGPVERHVPVDLRISGCPPRPSEIIKALSELRDGRPRPGD"
     gene            797..>4282
                     /locus_tag="VI359_06520"
     CDS             797..>4282
                     /locus_tag="VI359_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973940.1"
                     /translation="MRSFLFQPMRRQRHTSLALMTHSVLLASSLVVTPGLFAAGPGEA
                     SHEGHATPVSMPGWAEALKSQTLVEDAIEGRAGRSEKLEMQHNRLMRRLEEQAQKDAQ
                     AQQTSGAFNHTSMMHQYMGGDGSSFLLMTDSGKGEPVMTSGGKCPAGVPTKQYDVSMI
                     NIEITLNRWLDYYPGYMYVLTQDVEKARAEEAKNKAARDKDGYDPGAVSTGLQGDVIQ
                     PLVLRANQGDCVKMTLRNQMESEDGSLFIQASSMIVSATGKPATTTNPESIIAPGKAQ
                     EFEWYIHPHMQEGVRQFHSYSHDRELTVLGLFGAFIVEPKGSKYLDALGTGPAPETKS
                     GWQVMIDNGSGPDFREFVIFYHEIGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRALNY
                     RSEPFGIDQMHLQHEYFGFEDESMAYSGYTFGDPPTTLARGYLGEPVKWRIVHGGSEV
                     FHSHHPHSGSIRWQRSPVTEPNNLWAMGQDGPVKYPVVRTKSDRVDVEVIGPSEALDL
                     EPECGGGGCQHLAGEFLYHCHVAHHYVAGMWGYGRFYNTLQVGAGHTDTMPDMRELPD
                     RQGRMKLGVSSDKLIGTTVDWFGKTFKIVDKSQKTNWKSDPIIVNIKDWVEMFVPAQG
                     KPGHTDDEKGQIIAYDATVWDWKWNGNVAVGERESTAKYPKYPAAAKWDDSTRPAILF
                     DPTTGKMSWPLLKPHFGKRNPFSANHSGAPWLEPIHQDANGERTSEPAKPGEQGRWSL
                     CPENANQKFYNIHFVRMPITLAKKQGKEPAMVDKDGLIYVLHEEERLTRANDDLKFPA
                     VIRANVYDCVDLVLTSEWDDDDFTNFQSSKINIHPHFFQFDTGNSDGVISGMEYEMSV
                     RPFTMWGKPNKHGLPAPMVAKIVGGAKAGATSIKIQMAPGAALFHPNTELMVGMDCLE
                     KGNDPTVSLPRDKSCSEIARIKDIKGDQITFFKPLKQNHPAGDIVSPEFVRYRWWVDV
                     DLGTVFWHDHAFGATTWPHGGFGVTIVEPFGSTYHSPKDGKLVWSGPIADIHSNEPIG
                     AGVSGSFRELMVSIHDTVPHTVNIIEAGNPPGQPVEVALEAGKTVSFQMPEKILNAPN
                     KYINGGTHTTGSGLNFRAAPFAQRLSNNPDTSKLFSSAVHGDPGTPLLRAYTGDTMVF
                     RLLHQLMNESHVWTIS"
BASE COUNT          952 a         1196 c         1304 g          830 t
ORIGIN      
        1 catgcggacg cgttggtggt gacggggccg gttactgtga acatggagcg tgcgttgaag
       61 gatgtgtaca accagacgcc ggatcctaag atcgtgatcg cgcttggcga ctgtgccatc
      121 aattgcggta tgttcaaggg cagctatgca gttaccggtc cggtcgagcg gcacgtcccg
      181 gtcgacctgc gcatctccgg ctgcccgccg cgcccatcgg aaattatcaa ggccctatca
      241 gaactccgag atgggcggcc tcgtcctggt gactgaacga actcttcgag atcagccggg
      301 ccaatctgct cagcaatggc tgctggtacg acaattcatg agtttctgcc ccgaaactcc
      361 ccactttttc tcttcgacct gtccaagaga ttagacagtc caacgcgttg aaatcccaag
      421 atccttcttc ttgtcacacg gaacactgtc taacaacgta gaaattgtcc tcgaacgatc
      481 attatcgact caatgactgg tacgccacta actccacgtg ttcgcagcga aatttccatt
      541 gagatttctg tctcgctgtc ccggcacgtc gattgcgcta aaacagctcc gctggtgagg
      601 gtctgggtga ttttggaccg gcctccgaat gacccactcc ttcgcaagaa ggaacccttc
      661 accagctcta ataaggcaag cagatgtcaa gcgtgccgag tgtagttcgc acacgatcga
      721 agagacgaga aatgaacgac aaattgtttt taaccatccg ctcaacgagg cggacttata
      781 ggggggaggc cgtacgatga ggtcttttct atttcaacca atgcgcaggc agcgccacac
      841 cagtctggcg ctgatgacgc acagtgtgct gctggcaagc agcctggtgg tgacgccagg
      901 gttgttcgcg gcagggccgg gagaagcgtc ccatgaggga catgcgactc ctgtctcgat
      961 gcctggctgg gctgaggctc tcaagagtca gacattggtc gaggatgcga ttgagggacg
     1021 ggcggggcga tctgaaaagc tggagatgca acacaatcgt ctgatgcgcc ggctcgagga
     1081 gcaggctcag aaagacgcgc aggctcagca gacgtccggg gccttcaatc atacatcgat
     1141 gatgcaccag tacatgggcg gggatgggtc gagcttcctg ctgatgacgg attccggcaa
     1201 gggtgagccg gtgatgacgt cggggggcaa gtgtccggcc ggtgtaccaa ccaaacagta
     1261 cgacgtctcg atgatcaata tcgaaatcac gctgaatcgg tggctggact actatccggg
     1321 ctacatgtac gtcctgaccc aggatgtcga gaaagcgcgt gccgaggaag cgaagaacaa
     1381 ggccgcgcgt gacaaagacg gctacgatcc aggcgctgtg tccactggtc tccaaggaga
     1441 tgtgatccag ccgttggtcc tgcgtgccaa ccagggcgat tgtgtcaaga tgacgcttcg
     1501 caatcagatg gagagcgagg atggcagctt gttcatccag gcctccagca tgattgtcag
     1561 cgcgaccggc aagccggcga ccaccacgaa cccggaatcg atcatcgcgc cgggcaaagc
     1621 gcaggaattt gagtggtata tccacccgca catgcaggaa ggtgtgcggc agttccattc
     1681 ctacagccat gaccgtgaat tgacggtgtt gggtctcttc ggtgcgttta tcgtggagcc
     1741 gaagggttct aagtaccttg acgcgctcgg aacaggccct gcgcctgaga cgaagagcgg
     1801 atggcaggtg atgattgata acgggtccgg accggacttc cgcgaattcg tgattttcta
     1861 tcatgagatc ggcgacgaag ccttccgccc attgaacaag aagggcgact tcttgcccca
     1921 gcgcgatccg ttgaccgacg cctatcgtcc gggcggccgt gcgttgaact atcgcagcga
     1981 gccgttcggg atcgatcaga tgcacttgca gcatgaatat ttcggcttcg aagacgaatc
     2041 gatggcctac agcggctaca ccttcggcga tccgccgacg acgcttgcgc gcgggtattt
     2101 gggcgaaccg gtcaagtggc ggatcgtgca tggtggatct gaagtcttcc actcgcacca
     2161 tccccatagc ggatcgattc gctggcagcg cagcccagtc acagagccga acaatctctg
     2221 ggcgatgggt caagacggac cggtcaagta tccggtcgtg cggaccaagt ctgatcgtgt
     2281 cgacgtggaa gtcatcggcc catccgaagc gctcgacctc gagccggaat gtggcggcgg
     2341 cggctgccag catctggcag gtgaattcct ttaccactgc cacgtcgcgc accattacgt
     2401 ggcgggcatg tggggctatg gccgattcta taacacgtta caggtcggcg cgggccacac
     2461 cgacacgatg ccagacatgc gggaattgcc ggatcgtcag ggccggatga agctcggcgt
     2521 gtcgtccgac aagctgatcg ggacgacggt cgattggttc ggcaagacct tcaagattgt
     2581 cgacaagagc cagaagacca attggaaatc cgatcccatc attgtgaaca tcaaggattg
     2641 ggttgagatg tttgttccgg cccagggcaa gccgggccat acggacgacg aaaagggcca
     2701 gatcatagcc tatgacgcca ccgtctggga ttggaagtgg aacggcaatg tggctgtggg
     2761 cgaacgggag agcacggcga aatatccgaa gtatccagct gcggcgaagt gggatgacag
     2821 caccaggccg gccattctct tcgacccgac cacgggcaag atgtcttggc cgttgttgaa
     2881 gccccacttc ggcaagcgga atccgttctc tgcaaaccac agcggggcgc catggttgga
     2941 gccgattcac caggatgcga acggtgaacg gacctccgag ccggccaagc caggcgagca
     3001 ggggcggtgg agcctctgtc ctgagaatgc caaccagaaa ttctacaaca tccactttgt
     3061 ccgcatgccg atcaccctgg ccaagaagca gggcaaagag ccggctatgg tggacaaaga
     3121 cggcctgatc tacgtgctcc atgaagagga gcgcctgacc agagccaacg acgacctcaa
     3181 gttcccagcg gtcatccgcg ccaacgtgta tgactgcgta gacttggtcc tcaccagcga
     3241 atgggacgac gacgacttca cgaacttcca gtcgtccaag atcaacatcc atccccattt
     3301 cttccaattc gatacgggga actcggacgg cgtgatctcg ggcatggaat acgaaatgtc
     3361 ggttcggccg ttcacgatgt ggggcaagcc caacaaacac gggttgccgg ctccgatggt
     3421 cgcgaagatc gtcggcggtg ccaaggcagg agccacctcc atcaagatcc agatggctcc
     3481 cggtgctgct ctgttccatc cgaacaccga attgatggtg ggtatggatt gcttggagaa
     3541 gggcaacgac ccgacggtct cgctcccgcg ggataagagc tgttccgaaa tcgcccgcat
     3601 caaggacatc aagggcgatc agatcacgtt cttcaagccg ctaaagcaga atcacccggc
     3661 gggcgacatc gtgtcgccgg agttcgtccg ctatcgctgg tgggtggacg tggatttggg
     3721 gactgtgttc tggcacgacc acgcgttcgg tgcgacgacc tggccgcatg gcgggttcgg
     3781 cgtaacgatc gtggagccgt tcgggtccac ctatcacagc ccgaaagatg gcaagctggt
     3841 gtggagcggt ccgatcgcgg acattcacag caacgagccg atcggcgccg gtgtcagcgg
     3901 cagcttccgt gagttgatgg tctccatcca cgacacggtg ccgcatacgg tgaacatcat
     3961 cgaagccggc aatcctccgg gacagccggt tgaagtggcg ttggaagcgg gcaagacggt
     4021 gtcgttccag atgccggaaa agatcttgaa cgcaccgaat aagtacatca acggcgggac
     4081 gcatacgacc ggcagcggcc tcaatttccg cgcggcaccg tttgcacaac gcctctccaa
     4141 caatccggat acgtcgaagc tgttcagcag tgcggtccat ggagacccgg gtacgccgtt
     4201 gctccgtgcg tacacggggg acaccatggt gttccgcctg ttgcaccagc tgatgaacga
     4261 atcgcacgtg tggacgatct cc
//
LOCUS       DATDNW010000269         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_580769, whole genome shotgun sequence.
ACCESSION   DATDNW010000269 DATDNW010000000
VERSION     DATDNW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580769"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VI359_06525"
     CDS             <1..770
                     /locus_tag="VI359_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEY6973941.1"
                     /translation="QNLVDLRTHYRVTANTLVIDLRRPSKVLSSAPQGGGLRSVRYIL
                     NHQVTVPAGANCTTAAVKKTRQPEWRDPARYLRRLATAVGVDNNCVALMTAVPMKQLV
                     IGHEESQGIWVECFATVGITNAVRAGEWPGIDSDPKRDACLGTINLILVTNTTLSSAA
                     MVGAVQVATESKTGILRDHAVPSWSGVAGATGTGTDAVVVACALRGKGARLSYSGTHT
                     IIGAMIGRVVGECVTKGLARAASWIAHAASQSRSVST"
     gene            1592..2323
                     /locus_tag="VI359_06530"
     CDS             1592..2323
                     /locus_tag="VI359_06530"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6973942.1"
                     /translation="MRKLAMGLIAMGMVALSFTWTSSATAGDEAETAELLITLLKAGR
                     GVVSENQGLINDASKAHKGFTDDVFAQQALEKFKAKTKIDLAKAGNLPQGPMLKALLD
                     SEKEVVAEAQMVINKQGIGFKGFLPAVFARKTGERFYQKTGVKLRLTGLDYRNPGNKP
                     DDFEQEVLRMFADPRHPKGQQYVKTTMVAGKPVLRVMDPEYAGATCLSCHGSPKGDRD
                     ITGMKKEGWKEGDLAGAISLTLPIK"
BASE COUNT          608 a          587 c          743 g          569 t
ORIGIN      
        1 gacagaatct cgtggacctg cgtacgcact accgagtaac cgcgaatacg ctcgtgatcg
       61 atcttcgtcg tccttcgaaa gtactctcgt ctgctccgca agggggagga ctccgatcgg
      121 ttcgctacat tctcaatcac caggtcacgg tacctgctgg cgcgaactgt acgacagccg
      181 cggtcaagaa aacacggcag cccgaatgga gagatcctgc gcggtatctt cgccgactgg
      241 cgactgccgt gggtgtcgac aacaattgcg tcgctctgat gaccgccgtt cctatgaaac
      301 aactcgtgat cggtcatgag gaatctcagg ggatctgggt cgagtgtttt gcgacggtcg
      361 gaatcaccaa tgcggttcga gcaggggaat ggccaggcat tgattctgat ccaaagaggg
      421 acgcttgcct cgggacgatt aacctcattc tggttacgaa cactactctt tccagtgcgg
      481 ccatggttgg cgcggtgcag gtggcaactg aaagcaagac cggaatttta cgtgaccatg
      541 ccgttccaag ttggagtggt gtggccgggg caacaggaac cgggacagac gcggtggtcg
      601 ttgcatgtgc gcttcgagga aaaggtgctc ggctgtccta tagtggcacg cataccatca
      661 tcggcgccat gattggtcgg gttgtgggcg agtgtgtcac gaaaggttta gcacgtgcgg
      721 cgagctggat tgcccacgct gcgagccaat cacggtcggt ttcgacgtaa gaagcgaatg
      781 aagcatcctc acaactagcg gtcacggaat tctccagaaa gatggcgggc aggatggtga
      841 ttatgatggc gctgttcgcc atgatggtct cgtgtgggag acgtatatgt atgggctttg
      901 tgacgatcct ggatttcggt cgatgtggtt gaaccgtgtt cgagtgagaa aaggattatc
      961 gtccctctcg attcatgtat cggaagacat tgcgaaacag ttgtgaggtg gacttggtcc
     1021 atgggatcac catatcgaca tccacttgaa tctgatgctg cttctcaagg atcttctgtg
     1081 acgctgtctc ctatttcata gatccccctc ctgtattgaa tcaatcatgt gcacccctgt
     1141 gtctcttctg catgcatggg tcctctccag agataaacct cagtcagtcg atcaatgtgc
     1201 caagtccaaa attcggctgt gatgcacaac tgtgcacaaa tgggaaacgg atggtcaggg
     1261 ttgtgacagt cgacagcgtt tacgacagtt gttgttgatt tgacagcatt tgtgcaggtc
     1321 aggcgtgtgg gtgagaaaac tttcttgtgg gcagtacgta gtgtcggcac gtaagttgct
     1381 ggagttgtgc gcggaacctg cggtgccgtg tgtgctagtg cggtgaccat atgtgcgccg
     1441 atgctggcag tggcacgtca gcgccccata gagagaggga tgcagattct attgattcac
     1501 ttgccaatag agaatgaacc gctacactca ggtttacaca acaatgtcgt gacctgatca
     1561 cggctgttga tccgacgaag gaggaagaga tatgcgcaaa ctcgcgatgg gcctgatcgc
     1621 tatgggaatg gtagcgcttt cgtttacgtg gacatcgtcg gcaacggctg gagacgaggc
     1681 ggaaaccgcc gaactgttga ttacactgct gaaagccggg cgtggggtcg tatcggaaaa
     1741 tcaggggctc atcaacgacg cctcgaaagc tcacaaaggg ttcactgacg atgtcttcgc
     1801 gcagcaagcg cttgagaagt tcaaggcaaa aacgaagatt gatctcgcca aggcgggcaa
     1861 tctcccgcaa ggtccaatgc tgaaggctct tctcgactcg gagaaagaag tcgtcgccga
     1921 agctcaaatg gtaatcaaca agcagggcat cggattcaag ggattccttc ccgcggtgtt
     1981 cgcgagaaaa actggggaac gattttatca gaaaacaggt gtgaaactaa ggctcaccgg
     2041 gttggactat cggaatcctg gcaacaagcc ggatgacttt gaacaggaag tcttgcgcat
     2101 gttcgccgat ccacgccatc ccaagggtca acagtatgtg aagaccacga tggtagctgg
     2161 aaagccggta ttgcgtgtga tggacccaga atatgccggg gccacttgcc tatcgtgtca
     2221 tggatcgccg aagggtgatc gcgacatcac ggggatgaaa aaggaaggat ggaaggaagg
     2281 ggatctggct ggagcgatca gtttgacgct tcccatcaaa taaacggatc acgggattac
     2341 aaagcttcat cacaacatca atcatcagga gggtaccaga catgagcaag attctagtcg
     2401 tagacgattc gtatgcggaa ttgcagatga tcgagggcta tctgaaagga gccagtcaca
     2461 ctgtggtctc atttcccaat tcggagaaac tcgaggataa ggtgatc
//
LOCUS       DATDNW010000270         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_580948, whole genome shotgun sequence.
ACCESSION   DATDNW010000270 DATDNW010000000
VERSION     DATDNW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580948"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..2262
                     /locus_tag="VI359_06535"
     CDS             442..2262
                     /locus_tag="VI359_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY6973943.1"
                     /translation="MLLKRWLVGNPLKTAQAVHERLSKRLALAVFSSDALSSVAYATE
                     EILLVLVPASLAFAHFSIPISLMIVLLLAILTLSYSQIIFEYPEGGGAYIVSKSNLGE
                     WPGLTAAAALMIDYVLTVAVSVAAGIAALTSAIPPLFPHRTALGVFAILLVLLVNLRG
                     VRESGKVFAIPTYMFIGALLLMLAAGAFQFLSGRMSPFTPQNVPAQAAVESVSLFLLL
                     RAFSSGCTALTGVEVISNGVTAFRPPESKNAALTMAGMAVILATLFMGISTMAYYLGV
                     IPKEDETVISQVARAIFGAGPLYYLVQATTMMILILAANSAFAGFPRLASLLARDSFM
                     PRQMAMMGDRLVFSNGIIILGAFSGFLIVLFESDTHALIPLYAVGVFLSFTLSQAGMV
                     RRWLIKRGPHWKKKLVINGIGAATTAIATTIIASTKFVHGAWIVIVLIPLFIMMFRGI
                     RSHYKAVTEQIALSRGHRPPIPRRNIVLIPIGGVNRAVIRAVDYARSRPGEIRAVLVD
                     LDSEDTAKAEMQWAQWGCGVNLVVLPSPYRSILGSLLDYIEEILEKEPDSWVTVVIPE
                     ILPARWWQNILHNQRALMLKAALLFKDRVILTDVPFHLTR"
     gene            2265..3386
                     /locus_tag="VI359_06540"
     CDS             2265..3386
                     /locus_tag="VI359_06540"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY6973944.1"
                     /translation="MKNTPINAECNNWVLNVCLVACVCVLGAGVQPVHAFKIVTPAEH
                     SKLKPGQSITAQVDLGSDSGIVAVRYYWYPEQAETLVQQDESESAGGQSRNKIAVDKY
                     LEKDSVFGSVVAVPTLTSTANGTPPFGGSLKVPKEAIGPMRLLVVADISRGRLGTKSV
                     FDEIIVQVEPEAQLTNIDFETDKPLNLGRKGQLATYGQVDVLGKIFELPVVGEFADGV
                     TRPLSAPSSGTSYQSSNEKVIQVLQDGLLKIVGNGKTVLTVTNRGKQASLDVTVEVGD
                     EPNEPPVAVAGPNRTVKAGTKVELNGLKSTDPEGEALFYTWSQVRGSKVPLLDLNMQK
                     ASFVAPSVSEPKLFRFKLRVTDKKGADSAPAFMDVTVEP"
     gene            complement(3403..>3979)
                     /locus_tag="VI359_06545"
     CDS             complement(3403..>3979)
                     /locus_tag="VI359_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HEY6973945.1"
                     /translation="DRDNLDLIDDLIHENSHHHLNLLLRKHVLFHGDHNQQIFYSPWR
                     RSLRPLRGILHATFTFTMGAILFERLSSWGARGKRPGASGKKRSTRSGIELTKTQQLR
                     ARFRCLEELESVRYSIQDLEYADRHLGWVTGSGQRLVGQLADAIDKVERRIGPFEKAV
                     LRSKFGPALRRHAEELRKARQSYGPVRLSRA"
BASE COUNT          819 a         1186 c         1075 g          899 t
ORIGIN      
        1 gaagtctcag cggatacaag cggagcccaa agcagggcta tagctgccat cgcaccgagc
       61 gtacgggtgg atacacaaac cctttgtttg tggtggggaa acatggaccg gcctccttga
      121 tgtaaagagt gaaacaaacc cgaacctgtt gaacctaaat gatgaccgaa ggattcctcg
      181 attacagtct tcgccgtcag acgaagagat atttttgcga caaacggaac gggaaagata
      241 ctcaagttca cagactctgt caatcccctg aatctcggca tccatgaaca aaccaacgac
      301 atgcggacga taccctgccc gcaccttcga taactgctcc gaaccgtcat attgccggct
      361 tctcgcctga gccctttgac ctattctttc caagccctca taacattcac gcaatcgcgt
      421 catttgtgcg ataatccttc catgctcctc aagcgttggc tcgttgggaa ccccctcaag
      481 accgcccagg cagtgcacga acggctctca aaacgtctgg ccctcgccgt cttttcttcc
      541 gatgctcttt cctcagtcgc ctatgccacg gaggaaattc ttctggtcct ggttccggca
      601 agcctcgctt tcgcccattt ttcgattccg atcagtctga tgatcgtctt gttgctcgcg
      661 atcttgaccc tatcgtactc ccaaatcatt tttgaatatc ccgaaggcgg tggtgcctac
      721 atcgtctcca aatcgaacct gggcgaatgg cctggcctga ccgccgctgc cgcgctgatg
      781 atcgattacg tcctgacggt tgctgtcagc gtggccgccg gcatcgcggc cctcacatcg
      841 gccattcccc cactgttccc tcatcgtacc gcgcttggag tcttcgccat cttgctcgtg
      901 ctgctcgtca acctgcgggg agtccgtgaa tcgggaaagg tatttgccat accgacctat
      961 atgtttatcg gcgcgctctt attgatgctg gcggctggag cctttcaatt cctatccggc
     1021 cggatgagtc cattcactcc gcagaacgtt cctgcgcagg cggcggttga gtccgtctcg
     1081 ctgtttctcc tgcttcgcgc cttttcttcc ggttgtaccg cgctcaccgg tgtagaggtc
     1141 atttcgaatg gagtcaccgc ctttcgtcca cccgagtcga agaacgccgc gctcacgatg
     1201 gccggtatgg cggtcattct cgcgaccctc ttcatgggca tcagcactat ggcttactat
     1261 ttgggggtca tcccgaagga agatgagacg gtcatctcgc aagtcgctcg cgccatcttc
     1321 ggcgcgggtc ccctctacta tttagtccaa gccacgacga tgatgatctt aattctggcc
     1381 gccaacagcg cctttgcggg atttccccgt ctcgcgtcct tgctcgcccg cgacagtttc
     1441 atgccccgtc agatggcgat gatgggcgac cggctcgtat tctcgaacgg catcatcatc
     1501 ttgggagcct tctccggctt cctcatcgtg ctcttcgaaa gtgacaccca cgccctcatc
     1561 ccactgtatg ccgttggcgt cttcttgtcc ttcaccctct cgcaggccgg tatggtccgc
     1621 cgctggctga tcaagcgcgg cccccactgg aaaaagaagc tcgtcatcaa cgggatcggc
     1681 gccgccacca cggccattgc gacgacgatc atcgccagca cgaagttcgt gcatggcgcg
     1741 tggatcgtca tcgttctcat tccgttgttc attatgatgt tccgcggcat tcgctctcac
     1801 tataaggcgg tgaccgaaca aatcgccctc agtcgcgggc atcgtccacc gatcccgcga
     1861 cgcaatatcg tcctcatccc catcgggggc gtgaaccgag cggtgatccg cgccgtggat
     1921 tatgcgcgca gccgaccggg ggaaattcgc gccgtcctcg tggatctcga ttccgaagac
     1981 acagccaagg cggagatgca atgggcgcaa tggggctgcg gggtaaattt ggttgtcctt
     2041 ccctcgccct atcgttcgat tctcgggtca ttgctcgatt acatcgaaga gatccttgag
     2101 aaggagccgg acagttgggt caccgtggtg atcccggaaa tcctgccggc ccgctggtgg
     2161 cagaacattc tgcacaatca acgtgccctg atgctgaagg cggcgctcct gttcaaagat
     2221 cgcgtgattc tgacagacgt gccgttccac ctgacgaggt gattatgaaa aacacgccaa
     2281 tcaatgccga atgtaataat tgggtgctga atgtctgcct cgtggcctgt gtgtgtgtgc
     2341 tcggcgcagg tgtacaaccg gtccatgcct tcaagatcgt cacccccgct gaacacagca
     2401 agttgaaacc agggcaaagc attaccgctc aggtggatct ggggtccgac agcggcattg
     2461 tcgcggtccg ctactattgg tatccggaac aggcggaaac cctcgtccag caggacgagt
     2521 ctgaatcagc cggcggccag agccgcaata agatcgcggt ggataaatat ctggagaagg
     2581 atagtgtgtt cggctccgtg gtcgctgtgc cgactctgac ctctacagcc aatggtactc
     2641 cgccgtttgg aggcagcttg aaagtgccga aagaagcgat cggtccgatg cggttattgg
     2701 tggtggcgga catttcccgc ggcaggctcg gcacgaagtc ggttttcgat gagattatcg
     2761 tgcaggtgga gccagaagcc cagctcacca acattgattt cgaaacggac aagcccttga
     2821 acctcggacg caaaggacag ttggccacgt acggccaagt ggacgtgctt gggaagattt
     2881 tcgaacttcc cgtggtgggg gaattcgccg acggcgtcac gagaccgctc agcgcgccat
     2941 cgagcggcac aagctaccag tcatcgaacg agaaagtgat tcaagtcttg caggacggcc
     3001 tgctgaaaat cgtcgggaac ggtaaaaccg tgctgaccgt cacgaatcga ggcaaacagg
     3061 cttcactcga tgtcactgtc gaagtaggtg acgaaccgaa tgaaccaccc gtcgctgtcg
     3121 caggaccgaa tcgtaccgtg aaagccggca ccaaggttga actcaacgga ctcaagagca
     3181 ccgatccgga aggtgaagcc cttttctata cctggagtca ggtgcgcggg agcaaggtcc
     3241 cgctgcttga tctcaacatg cagaaagcct cgttcgtggc cccgtccgtg tcagagccaa
     3301 aactctttcg ctttaagctg cgcgtcacag ataaaaaagg tgcggatagc gcgccggcgt
     3361 ttatggacgt caccgtcgaa ccctagcata cttcatctta cgtcaagctc gactcagtct
     3421 caccggtccg tagctttggc gcgccttcct caattcctcg gcatggcggc ggagcgctgg
     3481 gccgaacttt gatcggagaa ccgccttctc aaatggcccg atgcggcgtt cgaccttgtc
     3541 gatagcgtcc gccagttgtc cgaccagtcg ctggcctgag ccagtcaccc agcccaaatg
     3601 tcggtcggca tactccaagt cttgaatcga atagcggact gattccaact cttcgagaca
     3661 gcggaagcgg gcgcgcagtt gttgcgtctt cgtgagttcg atgccgcttc gggtcgaccg
     3721 tttcttcccg cttgcccctg gcctcttgcc tctcgccccc cacgacgaca gccgctcaaa
     3781 gagtatcgca cccatcgtaa aggtaaacgt ggcgtgcaaa attccccgca gtggtcgaag
     3841 gctacgccgc cacggggaat aaaagatctg ctgattatgg tcgccatgaa acaggacatg
     3901 ctttcgcagc agcagattca aatggtgatg gctgttttca tggatgaggt cgtcgatgag
     3961 gtccagattg tcccggtca
//
LOCUS       DATDNW010000271         3876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_508580, whole genome shotgun sequence.
ACCESSION   DATDNW010000271 DATDNW010000000
VERSION     DATDNW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508580"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3283
                     /locus_tag="VI359_06550"
     CDS             <1..3283
                     /locus_tag="VI359_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6973946.1"
                     /translation="WGLRVEVGALEETGLMVSRLLDASLRQRMLIVIIAIMLGTGGIY
                     AFQTIPIDAFPDVTSVLVQVVTKAPGLSPAEVERLVTYPIELQLTGVPSLTELRSLTK
                     VGLSLVTIVFDDAMDINLARQLVLERLIEVKELLPPGAEPMLIPNSTGLGEVFQYYLE
                     GSSDPGMDTETYTRALIDQRTVQDWVIRPLLKSTPEVIDVNSMGGYVKQYQVLVEPVL
                     LRKYNLTLHDVFEAVANNNANAGGNILEKHAEKYIVRGVGLIRSLNDIERIVVKEAGG
                     TPVFVGDVAQVQIDHAVRHGATVLNGEREVVSGIVLMLRGGNAREVVQGIKARIEDIH
                     DRNLLPHGLRIVPFYDRIELITAALNTVYKSLAEGVVLVVVVLFLFLGNVRSALIVVS
                     TLILAPLATFIVMYYAGLTANLMSLGGLAIAIGMIVDGSVVVVENVYRHLSSHHAGTV
                     PKGELIASAAKEVGRPVVFGIVIIIMVFLPVLTLHGMEGKMFKPLAYTIMIALLVSLI
                     LSLTLSPVLSSLMLKRGSEKDPWIVRWAKRLYTPLLRMALGNGTLVLTTAVGLLACSL
                     ALFPFLGSEFIPILNEGSVAPQTIRLPSVSLQASIDIEKDMQRTLMEFPEVEMVVSKI
                     GRTELGNDPQEPNESDPVVRLRPLEQWTTARTMPELMQRFRERLMHVPGATFLISQPI
                     QQRVDELISGVRTEATVKLFGDDLEVLRHKADEIAGVLGTVRGVRDIKVEQLYGQPYL
                     TIDIDRSKIARHGINVADVREVITTAIGGRAATRVYEGQQRFDLVVRFPEQYRNSIET
                     ISNILLTDAAGALIPLADLGTVQLEEGPGRISRERLHRYVSIGFNTMGRDIGSLVAEA
                     QQNIAAQVQLPTGYTIAWGGSFENMERAMARLRVIVPLTIVLIFLLLYSSFDSLRQAA
                     LIILNLPFAMIGGLVGLWLTGEYLSVPASVGFINLFGVAVLNGIVLVSCMNKLREDGH
                     TLDEAVMSGALLRLRPVLMTALVALLGLVPLAFAQGIGSEVQRPLAIVVIGGLVSSTL
                     LTLIVLPVLYRWMERRSAEICVAQRDRRSEPEGESANSVVPQQQTTARKPEVLHRVSD
                     YGPAE"
     gene            3334..3648
                     /gene="sugE"
                     /locus_tag="VI359_06555"
     CDS             3334..3648
                     /gene="sugE"
                     /locus_tag="VI359_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEY6973947.1"
                     /translation="MNWLYLVIAGAFEIAWAIGLKYTEGFTRVWPSTGTVMAMIASFY
                     FLAQALKTIPVGTGYAVWTGIGAAGTAILGIVLFAESTALPRLVSLMLIVLGIVGLKV
                     ST"
     gene            complement(3672..>3876)
                     /locus_tag="VI359_06560"
     CDS             complement(3672..>3876)
                     /locus_tag="VI359_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973948.1"
                     /translation="SHEVQYWVKVVFVDNQELMVKDAIRHTISDDMEVLEVDTPKEVF
                     IIPMKQLKYLSCDATVFAGKKPS"
BASE COUNT          788 a         1084 c         1129 g          875 t
ORIGIN      
        1 atggggcctt cgtgttgaag tcggagctct tgaagaaaca ggcctgatgg tttcccgtct
       61 gcttgacgcc tccctccgcc agcgcatgct catcgtgatc atcgcgatca tgcttgggac
      121 agggggaatc tacgcatttc agaccattcc tatcgacgcc ttccccgacg tcaccagcgt
      181 gctcgtacaa gtggtgacca aggcaccggg cctctcaccc gcagaggtcg aacgactggt
      241 gacgtacccg atcgaactgc aactgacggg agtcccgtcc ctgacggaac tccgatcctt
      301 gaccaaggtc ggcctctcgc tggtgacgat tgtcttcgac gatgcgatgg acatcaatct
      361 ggcccgccaa ttggtgctcg aacggttgat cgaggtcaag gagttgttgc cccccggtgc
      421 ggagccgatg ttgatcccga atagcacagg acttggagag gttttccagt attacctcga
      481 gggatcctct gatcccggca tggacaccga aacctatact cgtgcgttga tcgatcagcg
      541 cacagttcag gactgggtca tccggccgtt gctgaaaagt acgccggagg tcattgacgt
      601 gaattcgatg ggagggtacg tcaagcaata ccaagtcttg gtcgaaccgg tcctgctgcg
      661 taaatacaac ctcaccctcc acgatgtctt cgaagcggtg gcgaacaaca atgccaacgc
      721 tggcggcaac attttggaga aacacgcaga aaagtacatc gtccgtggag tcggattgat
      781 ccgatcgctc aatgatatcg agcgaatcgt cgtgaaggaa gccggcggga cgccggtatt
      841 cgtcggagat gtcgcacagg tccaaattga tcatgcggtc aggcacggcg ccaccgtgtt
      901 gaacggggag cgggaagtcg tgagcggaat tgttctgatg ctgcggggag gcaatgcccg
      961 cgaggtcgtc caaggcatta aggcacgcat cgaggacatt cacgaccgaa acctgttgcc
     1021 gcacggcctg cgcattgtcc cattttatga ccggatcgag ctgatcaccg cagcgctgaa
     1081 taccgtgtac aagtcccttg ccgaaggggt cgtgctggtc gttgtcgtat tgtttttgtt
     1141 cctgggcaac gttcgaagcg cgctgatcgt cgtcagcacc ctcatcctgg ctcccttggc
     1201 gaccttcatc gtgatgtact atgcgggctt gacggccaat cttatgtcgt taggggggtt
     1261 ggccatcgcc atcggcatga ttgtggacgg ctccgtcgtc gtggtcgaaa acgtctaccg
     1321 ccacctctcg tcacatcatg cgggcaccgt cccaaaggga gagttgattg cctcggccgc
     1381 aaaagaagtc ggacggccgg tcgtgttcgg catcgtgatc attatcatgg tctttctgcc
     1441 ggtcctgacg ctgcacggga tggaaggcaa gatgttcaag ccactcgcct ataccatcat
     1501 gattgcattg ctggtgtcgc tcatcctgtc gctcacactg tcaccagtac tcagttccct
     1561 gatgttgaag cggggaagcg agaaagatcc gtggattgtt cggtgggcca aacgcctcta
     1621 cactcccctc ctccggatgg ctctgggaaa tgggaccctc gtgctcacca ctgccgtcgg
     1681 actgctcgcc tgcagcctgg cgctctttcc atttctgggg agcgaattca tccccatcct
     1741 gaacgaggga tcggtcgccc cccagacgat ccgacttccg agtgtgtcgc ttcaggcctc
     1801 gatcgacatt gaaaaagaca tgcagcggac actgatggaa tttcccgaag tggagatggt
     1861 cgtctcaaag attgggcgca cggagttggg aaacgatcca caggagccga atgaaagcga
     1921 tcctgtggtg cgtcttcgcc cgctcgaaca atggacgacc gcccgcacga tgccggagtt
     1981 gatgcagcga ttccgcgaac gattgatgca tgtccccggt gccacgttct tgatcagcca
     2041 accgattcaa caacgggtcg acgaattgat ttcaggggtg cgaacggaag ccaccgtcaa
     2101 gctgtttggc gatgatctgg aggtgttacg acacaaggcc gacgagatcg ccggcgtcct
     2161 ggggaccgtg cggggggtac gagacatcaa agtggagcag ctctacggcc aaccctacct
     2221 caccatcgat atcgatcgaa gcaagatcgc ccggcacggc atcaacgtgg ccgatgtgcg
     2281 tgaagtgatt accaccgcga tcggaggacg cgcggcgaca cgggtctacg aaggacagca
     2341 gcgattcgat ctggtcgtgc gattccctga gcagtatcgg aacagcatcg agacgatcag
     2401 caatattctg ctcaccgacg cggctggcgc cctgattccc ctggctgatc tcggcacggt
     2461 gcaactcgaa gagggaccgg ggcgaatcag ccgtgagcgg ctccatcgct acgtgtcgat
     2521 cggattcaac accatgggac gcgacatcgg cagcttggtc gccgaggccc aacagaacat
     2581 tgccgcgcaa gtccagcttc caaccggcta cacgatcgcg tggggaggct cgttcgagaa
     2641 catggagcgt gccatggcgc gcctgcgggt gattgtgcca cttaccatcg tgctgatctt
     2701 tctgcttctc tattcatcgt tcgattcgct ccgtcaggca gctctcatca tcctcaatct
     2761 cccctttgcg atgatcggag gcctcgtggg gctctggctg acaggagaat acctcagcgt
     2821 accggcatcc gttggcttca tcaatctctt tggcgtcgcc gtgctcaacg ggattgtcct
     2881 ggtgtcctgt atgaacaagc ttcgcgaaga cgggcacacc cttgatgaag ccgtcatgtc
     2941 gggagccctg ctcaggcttc gcccggttct tatgactgcg ttggtggcgt tgttgggatt
     3001 ggttccgctc gccttcgcgc agggaatcgg gtctgaggtt caacgtccgt tggcaattgt
     3061 cgtgatcggc ggactggtga gttccacctt gttgacgctg attgtgctgc cggttctcta
     3121 tcggtggatg gagcgtcgat cagctgagat ctgtgtagcg caacgggatc ggcgatccga
     3181 gccggagggc gaatccgcaa attctgtcgt tccgcaacag cagaccaccg ctcgcaaacc
     3241 tgaagttctt caccgggtca gcgactatgg ccccgctgaa tgatctgcat cgggatggtt
     3301 tccgtgtctc atacatgaag gagggaacac agaatgaact ggttgtatct cgtcattgcc
     3361 ggcgcgtttg agatcgcatg ggcgatcggc ctgaaatata cggaagggtt cacacgagtg
     3421 tggccgagca ccggcactgt tatggccatg atcgcgagtt tctactttct ggcgcaggca
     3481 ctgaaaacca ttcctgtcgg aaccggctat gccgtgtgga ccggaatcgg cgcggctggc
     3541 acggcgatct taggcatcgt tttgtttgcc gagtcgacag cgttaccccg actcgtctcc
     3601 ttgatgctga ttgtcctggg catcgtgggg ttgaaggtga gtacgtagca cgagaatgcg
     3661 ccttgatgcg tctagctcgg ttttttaccg gcaaacacgg tcgcatcaca gcttaaatat
     3721 ttgagctgct tcatcgggat aataaacacc tctttgggcg tatcgacttc gagcacttcc
     3781 atgtcatcgc tgatcgtatg gcgaatggca tccttgacca tcaattcctg gttgtcgaca
     3841 aatactactt tcacccaata ttgcacctca tgactc
//
LOCUS       DATDNW010000272         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_581461, whole genome shotgun sequence.
ACCESSION   DATDNW010000272 DATDNW010000000
VERSION     DATDNW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581461"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VI359_06565"
     CDS             complement(<1..768)
                     /locus_tag="VI359_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6973949.1"
                     /translation="MLTFGSCASTPSIPPWFEALERIPVKTVTVNGQRMAYLDVGQGP
                     PVILIHGFGGSMWQWEHQQTVLSTKFRVITPDLVGAGLSDKPEIDYRPDELLAFLVGF
                     MDALQIPRADVVGNSMGAGVAIGMALDYPTRVSNLVLIDGFPARVMDHLSSPTVRRAL
                     ETSAPAWVVSFGNWLFGWTMTESILQEFIYDPALITPAVLDRSNRNRRRPGQFRSLLT
                     IGQNLPIWEEQFAPRIGKVTQRTLIVWGEEDRVFPVKA"
     gene            complement(848..2806)
                     /gene="oadA"
                     /locus_tag="VI359_06570"
     CDS             complement(848..2806)
                     /gene="oadA"
                     /locus_tag="VI359_06570"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HEY6973950.1"
                     /translation="MATRRSQTKKTARSRKQPPSPAIRAPKGRRARAEDLTIQPAHGK
                     PILITDVALRDGHQSLLATRLRTEDMLPIAQKLDAVGYWSLEVWGGATFDTCLRFLKE
                     DPWERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVLERFIERSAANGIDVFRIFDA
                     LNDIRNLDRAIHEVKACGKHVEAAICYTVSPVHSVDRYVDLARQMEDLGTDTLCIKDM
                     AGLLAPLDAYHLVRRLKAAVKVPIHLHTHYTSGMASMASLMAILGGLDMLDTAMSPLA
                     GGTSHPPTETMVASLRETPYDTGLDLAAFQAITEHFRAVRRKYRQFESDFTGVDAEIL
                     TSQIPGGMLSNLAAQLTEQNALERMKEVLDEVPRVRKELGYPPLVTPTSQIVGTQATL
                     NVLTGERYKVITTETKNYFLGLYGRAPGPVDADIMARAIGNEDPIRTRPADRLEPELE
                     PIRKDLPSSATTVEDHLSFALFPAIARDFFDAREKGELTPEPLEISSPKGPAVAHELH
                     LAPVEFNVNVHGETYHVKVSGSGRKTDGRKPYYIRVNDKLEEVSLETIQEVLTGVPEA
                     PDAGTGSKPKRPKPTKAGDVAPPMPGRVVKVLVHKDALVKTGDPLLIIEAMKMESRIP
                     APIDGRVTAILVAEGDNVKTDETLIQLE"
BASE COUNT          582 a          829 c          924 g          680 t
ORIGIN      
        1 cgccttgacc ggaaacactc ggtcttcttc gccccacacg atcagcgtgc gttgtgtcac
       61 cttgccgatg cgtggcgcga actgctcttc ccagatcgga agattctggc cgattgtgag
      121 gagagagcga aactgtcctg gccggcgacg gttgcggttc gaccgatcca gcacggcagg
      181 tgtgatcaac gcagggtcgt aaatgaattc ctgcaaaatg gattcggtca tggtccatcc
      241 gaagagccaa ttgccaaacg agacgaccca cgccggtgca ctggtctcta aagcacggcg
      301 gaccgtagga ctgctgagat ggtccatcac gcgggcggga aagccgtcaa tgagtaccag
      361 gttcgagaca cgggtgggat agtcgagcgc cattccgatg gcgactccag cgcccatcga
      421 attgccgact acatcggctc gaggaatctg aagcgcgtcc atgaacccca ccaagaaggc
      481 cagcagctcg tcgggtcgat agtcgatctc gggtttatcg gaaagccccg ccccaaccag
      541 atcgggagtg atcactcgga actttgttga aaggaccgtt tgctggtgtt cccactgcca
      601 catcgacccg ccgaacccat gaatcaaaat caccggtgga ccttgaccta cgtcgagata
      661 ggccattcgt tgcccgttga ctgtgacggt cttgacgggg attcgctcga gggcctcaaa
      721 ccatggggga attgatggtg tcgatgcgca gctgccgaac gtgagcatga tcgaccatcc
      781 taggatgagg agcaagcaga tgtagtctgg ctgatgaacc gaacgtccgt ggtcggcgac
      841 aaccaggcta ttcaagctgg atgagtgtct catccgtttt gacgttgtcg ccttcggcaa
      901 caaggatcgc agttactctt ccatcgatcg gagccgggat gcggctttcc atcttcatcg
      961 cctcaatgat gagcagaggg tcaccggttt tcaccagggc atccttgtgg accaggactt
     1021 tcacgacgcg tcccggcatc gggggagcta catcaccggc cttggtcggt ttcggtcgtt
     1081 tcggttttga accggtcccc gcatcgggtg cctccggcac gccggtgagc acttcctgga
     1141 tcgtttcgag agacacttcc tccagcttgt cgttgacccg aatgtagtac ggtttgcggc
     1201 cgtcggtctt ccggccggag cccgacactt tcacgtgata ggtttctccg tgtacgttca
     1261 cgttgaactc gaccggcgca aggtgtagct cgtgggcaac tgcgggccct tttggcgagg
     1321 aaatttccag cggttccggt gtcaactcac ccttttctcg tgcgtcaaag aagtctcggg
     1381 ctatcgccgg gaagagggcg aaggatagat gatcctcgac tgtcgtggca gacgatggca
     1441 ggtcctttct aatcggctcc agttcaggtt cgagccggtc agccggtcgg gttcgaatgg
     1501 ggtcttcgtt cccgatcgcc cgagccatga tatcggcatc gacggggccc ggggctcggc
     1561 catagagacc gaggaaataa tttttagtct ccgtcgtaat gactttgtac cgttcgcccg
     1621 tgaggacatt gagcgtggcc tgcgtgccga caatctggct ggtaggggtc acgagtggag
     1681 gatagcccag ttctttcctc acgcgcggaa cttcatcgag gacttccttc atgcgctcga
     1741 gcgcgttctg ttcggtcaat tgggcagcca ggttggagag catgcctccg gggatctgtg
     1801 aggtcaaaat ttccgcatcg actccggtaa agtcgctctc gaattgccga tattttctgc
     1861 ggactgcccg aaagtgttcg gtgatcgctt gaaaagccgc caaatccaaa ccggtgtcgt
     1921 aaggcgtctc gcgcagagat gcgaccatcg tttcggtcgg ggggtgcgag gttccccctg
     1981 ccaacggaga catggcggtg tcgagcatat caaggcctcc gaggatcgcc ataagagacg
     2041 ccatggaggc catgccggat gtgtaatgag tatgaagatg gatgggaacc ttgactgccg
     2101 ccttgagccg gcgcaccaga tgataggcat cgagcggtgc cagaaggccc gccatatctt
     2161 taatacacag cgtgtcggtg ccgaggtctt ccatctgacg tgcgaggtcg acgtaccggt
     2221 cgacgctgtg gacggggctg acggtatagc agatggctgc ttcgacgtgt ttgccgcaag
     2281 ctttcacttc gtgaatggcg cgatccaggt tgcgaatatc gttgagcgcg tcgaaaattc
     2341 taaacacgtc gatcccattg gcagccgacc gctcgatgaa tcgctcaagc acgtcgtcgg
     2401 catagtggcg gtagccgacc aagttctgcc ctcggagcag catttgcagt ttggtattgg
     2461 gcatggcggc tcggagcgca cgcagacgtt cccagggatc ttctttgaga aagcgcaggc
     2521 aggtatcgaa cgttgctccg ccccagacct ccaatgacca gtagccaacc gcgtcgagct
     2581 tttgcgcgat gggcagcatg tcttctgttc gcaggcgcgt ggccaacagc gattggtgcc
     2641 catcgcgcag ggccacatcg gtgatcaaga tcggcttgcc gtgggccggt tgaatcgtga
     2701 ggtcctccgc ccgagccctt cgtcccttgg gtgcgcggat cgcgggactc ggaggctgtt
     2761 ttcttgatct cgctgttttc ttagtctgtg atcgtcgcgt cgccattgct atcgttgggt
     2821 ttctgctccg aggtttgggg tgcgcgtggc gctgtctggt ctgctagagt ccttcatagg
     2881 cggcaatcgc cgctgatatg gccagtacca aatcctccgg ctgttcgaac tcgtcgtagt
     2941 tgaagagttc aggatgggtt tcgaggtagg acgtatcgaa gcggcctgcc agaaagtccg
     3001 gatcctgcat gatgg
//
LOCUS       DATDNW010000273         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1264389, whole genome shotgun sequence.
ACCESSION   DATDNW010000273 DATDNW010000000
VERSION     DATDNW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264389"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VI359_06575"
     CDS             <1..348
                     /locus_tag="VI359_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY6973951.1"
                     /translation="MLAGESDPQDIIRSVDHGLYAVSFGGGQVDITNGKFVFSASEAY
                     LIEGGKVTKPVKGATLIGSGPEILTKVSMVGHDLKLDEGIGTCGKEGQSVPVGVGLPT
                     IRIDEITVGGTQG"
     gene            365..1732
                     /locus_tag="VI359_06580"
     CDS             365..1732
                     /locus_tag="VI359_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY6973952.1"
                     /translation="MSHQLQQGNGYTQLATDVLRQAKRSGATEADIIIAEGETFSVQV
                     RLGAVDRLTKAREKQLGLRVFVGKRSASTSTSDFSNESLDGLVTQTCTLAKAVVEDPL
                     SGLPSADQMAIERPDLDLHDPTKLETDYQIDLARRAESAAMTADLRVTNSEGAEFDSS
                     SGRVVLGNTHGFIGEFRSSSFSLSVSPVATDQQTGTMQRDSWYGVNRKFSKLESPETI
                     GAEAARRTVRRLGARKVATQRVPIVFDQDTAASLMGTLSSAVSGYALYKGASFLIDHL
                     GKSIAPECVTVYDDGRMAGGLGSRPFDGEGLPTRKTTVVERGVLTSYLLDTYSAKKLG
                     LHSTGNASRSVGENPSVGPTNLYLVPGTKTAQEIIASVRRGLYVTELIGFGINMVTGD
                     YSRGVCGFWIENGELAYPVEEITIASTLQQMLAGIETVGNDLVFRGRIASPTVKIVEM
                     MVAGN"
     gene            1875..2339
                     /locus_tag="VI359_06585"
     CDS             1875..2339
                     /locus_tag="VI359_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY6973953.1"
                     /translation="MRISSTAFSNQTAIPALYTCEGKDISPPMAWSDIPAGTKSLALI
                     VDDPDAPDPAAPKMTWVHWVLYNLPATNGGLPEGVKVLPAGTKEGLNDWKRTGYGGPC
                     PPIGRHRYFHKLYALDTVLPDLKQPTKVKLEEAMKGHVIGEAQLMGTYQKSR"
     gene            2498..2956
                     /locus_tag="VI359_06590"
     CDS             2498..2956
                     /locus_tag="VI359_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973954.1"
                     /translation="MPLDAELGKKMLQLITSRYDDRHWRKKIEKTLSLPQSGVGDPVQ
                     QQIFMHLKIHLKAYKSRRADPDSWIVGGYATKEVIDRAKFQPSLVGPSLTKDDVAFLG
                     SDPGQEVDEAWWDEMLVAWFEVPEAEKSIGEDEEGESNQSKNLEDAKPSV"
     gene            complement(3006..3662)
                     /locus_tag="VI359_06595"
     CDS             complement(3006..3662)
                     /locus_tag="VI359_06595"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY6973955.1"
                     /translation="MTALTLQELAKSLHNCQRCKLAKLGRTQVVFGVGNPRASIMFVG
                     EAPGFYEDQKGEPFVGAAGKLLNDLLQSAGLSRDEIYIANVIKCRPPNNRDPEMDEVD
                     TCKPFLMQQIQMIRPKLVCTLGNWATQTLLERKVGITKVKAQAFYMKDFVLFPLLHPA
                     AALHQGGLLEPLKEDFKKLKEFLDRHTKPAEPTETAPAASPTLQIESPLPSQMDLFGS
                     "
BASE COUNT          898 a          959 c         1197 g          847 t
ORIGIN      
        1 atgctggctg gagagtctga tccgcaggat attattcgct cggtcgatca cgggctttat
       61 gccgtgtcgt ttggcggggg acaggtggac atcaccaacg ggaaatttgt cttttccgca
      121 agcgaagcat atctgatcga aggcgggaag gtgacaaaac cggtgaaggg tgcgaccctc
      181 attggcagcg gtccggagat tctgaccaag gtttcaatgg tagggcatga cttgaagctt
      241 gatgaaggca tcgggacttg tgggaaagag ggacaatctg tgccggttgg tgtcggattg
      301 cccaccattc gcatcgacga aataacggtg ggtggcaccc aagggtgatt cacgtgtgga
      361 ttcgatgagc catcagcttc aacaaggaaa tggctatacg caactggcca ccgatgtgct
      421 ccgtcaggcg aagcggtctg gtgcgaccga ggccgatatc atcatcgctg agggagagac
      481 cttttcggtg caggtccggc tgggagcggt cgatcgtttg acgaaagctc gagaaaagca
      541 gcttgggttg cgagtgttcg tagggaaacg atctgcctcg acatccacat cggacttttc
      601 gaatgaatca ttagatggat tggtaacgca aacctgtacg ttggcgaagg ccgtggtgga
      661 agaccccctg tccggtctcc ctagcgctga tcaaatggcg atcgagagac cggatctcga
      721 cctgcatgat ccgaccaagc tggaaaccga ctatcagatc gatttggcgc gacgggccga
      781 atccgcggcc atgaccgcgg atttgcgggt gacaaactcg gaaggggcgg agttcgattc
      841 ttcatcgggc cgggtggtac tcggcaatac gcatggcttc atcggggaat ttcggagttc
      901 gagtttttcg ctgtctgttt ctccggtcgc cacggatcag cagacaggca cgatgcaacg
      961 tgattcctgg tatggggtga accggaaatt cagcaaactg gagagtccag aaaccatcgg
     1021 cgcagaagct gcccggcgaa cggtccgtcg attgggtgcc aggaaggtgg ccacgcaacg
     1081 agtgcctatt gtgttcgatc aggacacggc tgccagctta atggggacgc tctccagtgc
     1141 ggtgtcgggg tatgctctgt ataaaggggc atcattcctc attgatcacc tcgggaagtc
     1201 gattgcgcca gagtgtgtga cggtgtatga cgatggccgg atggccggtg gtctcggctc
     1261 gcggccgttc gatggcgaag gtcttccgac acggaaaacc acggtggtgg agcgaggtgt
     1321 cctgacaagc tatctccttg atacctattc ggcgaagaag ctcgggttgc actcgacggg
     1381 taacgcatca cggagtgtag gggagaatcc ttcggtcggg cccaccaacc tctatcttgt
     1441 gccggggaca aagaccgcgc aggagattat tgcgagtgtc cgacgggggc tctatgttac
     1501 ggaactgatc gggttcggta ttaacatggt gacgggagac tattcccgtg gggtgtgtgg
     1561 attctggatc gaaaatggcg aactcgccta tccggtcgag gaaataacga ttgcgagcac
     1621 gcttcagcag atgttggcgg gcattgagac ggtagggaac gatctcgtgt ttcgagggcg
     1681 gattgccagt ccgaccgtca aaattgtcga aatgatggtt gcaggtaact gaccaagcac
     1741 aagagcgaat agccggagat cctttctagc tatcagacgg cggcatgagt cgacagagcc
     1801 accacgcatt ggtagcagcc gtgctcatag ctagcataac cgctgcggtt gctttggctc
     1861 gtgaggaggg caacatgcgc atcagctcga cggcgttcag caaccagact gcaattcccg
     1921 cattgtatac ttgcgagggt aaggacattt cacccccgat ggcgtggtcg gacatcccgg
     1981 caggcaccaa gagtctggcg ctgattgtcg atgacccgga cgccccggac ccagccgcgc
     2041 cgaaaatgac ctgggtacac tgggttctct ataacctgcc ggccacaaat ggcggactgc
     2101 cggaaggtgt gaaggtgctg cccgcaggaa cgaaggaagg cttgaatgac tggaagcgaa
     2161 ccggttacgg aggaccttgc ccgccgatcg gccgccaccg ctacttccac aagctctacg
     2221 cgctggacac cgtactgccg gacctgaagc agccgaccaa ggtaaagctc gaagaggcca
     2281 tgaagggcca cgttatcggc gaagcgcagt tgatggggac ctatcagaag agcaggtgaa
     2341 attcggccgc ctacgaaggc agcgccgggc cccggtcgac gagcgcgagc tgtcccgcta
     2401 cggcacctgc gaggaaagac caccgctcac ggccatggat acatgggccg gggaagtcga
     2461 ttttaaacaa cacttgatta gctagatagg aaacaccatg ccactcgatg ctgaattagg
     2521 gaagaaaatg ctgcagttga ttacgtcgcg gtatgatgat cggcattgga gaaaaaagat
     2581 cgaaaaaacg ttgagtctgc cacaatccgg tgtgggcgat cccgttcagc agcagatttt
     2641 catgcatttg aaaatccatc tcaaagccta taaatctcgg cgtgctgatc cggattcttg
     2701 gattgttgga ggatatgcga ccaaagaagt gatcgatcga gccaagttcc agcccagtct
     2761 tgtcgggccg tctctcacta aggacgatgt ggcatttctc ggcagcgatc caggccaaga
     2821 ggttgatgaa gcctggtggg acgagatgct cgtcgcctgg ttcgaggtgc cggaagcaga
     2881 aaaatctatc ggagaagacg aggaaggcga gtcgaatcag tcgaagaacc tcgaagacgc
     2941 taaaccttcc gtgtgacgag tctctcgcag ctttgagtaa gccgctgatg gttgatggcg
     3001 gcgagttacg atccaaacaa atccatttga gaaggcagtg gtgattcgat ctgtagcgtg
     3061 ggacttgctg cgggcgcggt ctcggtgggc tcggctggct tggtgtggcg gtcgaggaat
     3121 tccttcaact tcttgaagtc ctctttcaga ggctccaata atccaccttg atgcagcgca
     3181 gcagccgggt gaaggagtgg gaacaggaca aagtccttca tgtaaaaggc ctgcgccttc
     3241 accttcgtaa tgccgacttt ccgctccaag agcgtctggg tggcccaatt ccccaacgta
     3301 cagaccagct tgggcctgat catctgaatc tgctgcatca agaacggttt gcaggtgtcc
     3361 acctcatcca tttccgggtc gcgattgttc ggcggccggc atttgatgac attcgcaata
     3421 tagatttcgt ctcgcgacag ccccgccgac tgcaagagat cattcaagag cttccctgct
     3481 gctccgacaa acggttcccc tttctgatct tcatagaaac cgggcgcctc gcccacgaac
     3541 ataatgcttg cacggggatt accgacacca aaaacgacct gagtccgtcc gagtttggcg
     3601 agtttgcatc gctgacaatt gtgaagagat ttcgcaagtt cttggagggt caaggctgtc
     3661 atgattgaat aggatcggtg gaaaaagtgg cggtatctta gaaagacggg gctacgttgt
     3721 caatgtatga ggcagtgaaa acgagaactg tttatgagtg cgccaacgtg tattttcttt
     3781 tcagcctgtt cctgctggct tttccgttgg tcgcggcggc gggtcctata gagctgatgg
     3841 cttcgaagga taacatgtac tgtgaacagg ttcttgatct gtttcagagg aatatgggag
     3901 g
//
LOCUS       DATDNW010000274         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_723198, whole genome shotgun sequence.
ACCESSION   DATDNW010000274 DATDNW010000000
VERSION     DATDNW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723198"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VI359_06600"
     CDS             complement(<1..333)
                     /locus_tag="VI359_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973956.1"
                     /translation="MFGWSAGLLALGLWCGLSLDAQSSSASDAIDLYFNTPGTVQGPP
                     APSPTETTYARYGSFDNRLLVWFVTQQHTYFGGFVLALPIFCVLLEFLGLTTKKPALS
                     LRYDGLARD"
     gene            complement(360..1418)
                     /locus_tag="VI359_06605"
     CDS             complement(360..1418)
                     /locus_tag="VI359_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973957.1"
                     /translation="MLHRMALRVLPSLTLAVTEESVRKQKRIVMLVPGLVAFGVYRLA
                     KHLLPLSEPVVLLLVSGAVATVTSLMAYRVGRAESWPTIVRQDGNRLLAWLTGWIGFV
                     YGVQLSLLVLALLWLVGYDYLVHPDGPAMMAIIISCTAVARDAFEIGHVRNVAMKGRP
                     FPTFPDGAALRAMMGDRLARLGPWMIAGLVVGAAGAWPASLVANNHVAALVQLTTITV
                     LGAAVALFAYFGGFRSTTSWRRGLAETKAGELVKYWWWPGLAFTSTYYLVVVGALLFL
                     VKQPTISTMQASISGGLVTAMMAVYCYYLGYRRSIEDQQGQSLSGAVLRCPFVMGILG
                     KSPTAVMAAPVAPGSKGL"
     gene            complement(1427..1900)
                     /locus_tag="VI359_06610"
     CDS             complement(1427..1900)
                     /locus_tag="VI359_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEY6973958.1"
                     /translation="MHTEGIEERVYDDGVLQWFIRSECRGCGLKVGIDVPAGQTHELV
                     DRVIWTDDALHRLDRLPPYLAPLFREEVERDVRARGERVVAYDTLFRPRTGERIVWEP
                     EAERRLDNVPAPVRAMAKIELERTAAERGETRVTVALMEEVKAKYFGMAASKQGS"
     gene            complement(1931..2647)
                     /locus_tag="VI359_06615"
     CDS             complement(1931..2647)
                     /locus_tag="VI359_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEY6973959.1"
                     /translation="MEQLLKQPGFLGTHATLGADLSQLMATLFTGLFIIGWIQAKQRR
                     GHAHHWLMLGGMVAMLSFFVSYYWFRQLGALAFAGKEGFGGSQEMYDNFFWPLLKFHI
                     VLVIVGLIMAVYMIVLGFRAQAIDGMRRILAERILQTTWKKVGVILGAIAALVLLYFA
                     VLLISGRFSLGKAIVWVSFLVIVAAVLGLELSIQRMWPSGEARHRALGNFTMILYCVL
                     FVTGTVTYTMLYILYPGKIG"
     gene            complement(2650..>2948)
                     /locus_tag="VI359_06620"
     CDS             complement(2650..>2948)
                     /locus_tag="VI359_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY6973960.1"
                     /translation="VLMSDILHYQNFATNAALVQKVHSHLAPGGRLVIKDRFLDETGT
                     GPAWTTAFAVHILVNTQAGSCYKTADAIHWMTQAGFDSPIELERTAVVQGVKSK"
BASE COUNT          725 a          904 c          757 g          562 t
ORIGIN      
        1 atcgcgagcg agtccatcgt agcgaagaga caaggccggc tttttcgtgg tgagccctag
       61 aaactcgagt agcacgcaaa agatcggaag ggccaacacg aatccgccga agtaggtatg
      121 ttgttgggtc acgaaccaga cgagcagacg attgtcaaag gagccgtagc gggcataggt
      181 ggtttccgtt ggagaaggcg cgggtgggcc ttgcacggtc cctggtgtgt tgaagtagag
      241 atcgatggca tcgcttgctg aggaagactg agcgtccaga ctcaatccac accacaggcc
      301 aagcgcgagc aggccagcag accaaccgaa catcgagaga agcctcatgc gcgatatcat
      361 cacaagccct tcgatcccgg agccacgggg gccgccatca ccgcagtcgg ggattttccg
      421 agaatcccca tgacaaacgg acaacgaagc acggcgccag acagactctg gccttgttga
      481 tcttcaatgg atcggcggta ccccagatag taacaataga ccgccatcat ggcggtgacg
      541 agacctccgc tgatagaggc ttgcattgtg gagatcgtgg gctgcttgac cagaaacagc
      601 aacgccccca cgaccacaag atagtaggtc gacgtaaagg cgagtcccgg ccaccaccaa
      661 tatttcacga gctcgccggc tttcgtctcc gcgagccccc gcctccatga ggtcgtcgat
      721 cgaaaccctc cgaaataggc aaacagcgcg accgctgcgc cgagcaccgt gatggtcgtc
      781 aactgaacaa gcgcagccac gtggttattc gcgaccaaac tggctggcca ggcacccgct
      841 gccccgacaa ccaggccggc aatcatccac ggtcccagtc gagctaatcg atcccccatc
      901 atcgctcgta gcgcggcccc atcgggaaag gtgggaaatg gacgtccctt catggcgacg
      961 ttgcgcacgt gtccaatttc gaaggcatcg cgcgcgacgg ccgtgcatga aataatgatc
     1021 gccatcatcg ccggcccatc cggatgtacc agatagtcgt agccgaccaa ccagaggagt
     1081 gccagcacga gcaacgacag ttgcacgcca tagacaaatc cgatccaacc agtcagccat
     1141 gccagcaatc gattgccatc ctgccggaca atcgtcggcc aggactccgc tcgaccaact
     1201 cgataggcca tcaacgacgt cacggtggca actgcaccgc tgaccagcag taacaccacc
     1261 ggctcggaca acggcagcag atgtttggcc agccgataca caccaaacgc caccagcccc
     1321 ggcaccaaca tcacgatccg cttttgtttg cgaaccgact cttccgtcac ggccagagtc
     1381 aaactcggaa gcactcgtaa ggccattcga tggagcatcg tgattcctat gacccctgtt
     1441 ttgaggcagc catgccgaaa tacttggctt tcacttcttc catcagagcc acggtgacgc
     1501 gagtctctcc ccgttctgcg gccgtgcgct cgagttctat tttcgccatg gcacgaaccg
     1561 gggccgggac attgtccaaa cgacgttccg cttcaggctc ccaaacgata cgctctcccg
     1621 tgcgcggccg aaacaatgtg tcataggcca ccactcgttc gccgcgcgcg cgcacgtccc
     1681 gttcgacttc ctcgcggaac aacggagcca ggtaaggagg cagacgatcg agccgatgga
     1741 gcgcgtcatc cgtccatatc acccgatcga caagctcatg tgtttgcccg gccggcacgt
     1801 cgatccccac ttttagtccg cacccgcgac attcgctccg gatgaaccac tgaagcaccc
     1861 catcgtcata gactcgttct tcaatccctt ccgtatgcat ccagcggccg catccacagg
     1921 tcaacatggc tcagccaatc tttcctggat ataagatata gagcatggtg taggtaacag
     1981 tacccgtcac aaagagaaca caatacagga tcattgtgaa gtttcccaaa gctcggtgcc
     2041 gcgcttcacc actcggccac attctctgga tgctgagttc gaggcctaaa acagccgcga
     2101 cgatcacgag aaatgaaacc cacacgattg cctttccaag agagaacctc ccacttatca
     2161 gcagtactgc gaagtagagc aagacaagag ccgctatcgc accgagaatc actcccactt
     2221 tcttccaggt tgtctgtagg attctctcgg caaggattcg tctcataccg tcgattgctt
     2281 gcgcacgaaa tcccagcaca atcatgtaga cggccataat cagcccgaca atgactaaga
     2341 caatatgaaa cttaaggaga ggccagaaaa agttgtcata catttcttga gatccaccaa
     2401 acccttcttt gcctgcaaac gcgagggctc caagttgccg gaaccagtag tagctgacaa
     2461 aaaaactgag catggcgacc atgccgccga gcatgagcca atgatgcgca tgacctcgcc
     2521 tctgcttcgc ctgaatccat ccgataatga aaagcccggt aaagagcgtt gccatcagct
     2581 gactgagatc agctcccagt gttgcgtgcg tgccaaggaa accgggctgc ttcaataatt
     2641 gttccatggt tatttcgatt tcactccttg caccaccgcc gttcgttcca gttcgatcgg
     2701 tgagtcgaac ccggcttgag tcatccagtg aatggcatcc gctgttttgt aacaactacc
     2761 cgcctgcgtg ttcaccagaa tgtgcacggc aaacgcggtg gtccaagccg gaccggttcc
     2821 cgtttcgtcc aggaatcgat ccttgatcac caggcgacca ccgggcgcga ggtgcgaatg
     2881 gactttctga accaacgccg cgttcgtggc aaaattttga taatgcagaa tgtcggacat
     2941 gaggacga
//
LOCUS       DATDNW010000275         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_922042, whole genome shotgun sequence.
ACCESSION   DATDNW010000275 DATDNW010000000
VERSION     DATDNW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922042"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="surE"
                     /locus_tag="VI359_06625"
     CDS             complement(<1..753)
                     /gene="surE"
                     /locus_tag="VI359_06625"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY6973961.1"
                     /translation="MRILITNDDGILSPGLTALAKTLKVIGEVWVVAPDRERTAAAHA
                     VTLHKPLRVQQMDTRIYAVNGTPVDCVNLAVLKIMPKPPALVVSGINKGVNLGDDVMY
                     SGTVSAALEGTILGIPSIAVSQEGHERFRFDVGADYAIRIARLVLERGLPDETLVNVN
                     VPDRPLSAVRGVRVTCLSRRRFDNPIIEKVDPHGRSYYWIAGTRVSWSRSKDADHEAI
                     AEGLVSLTPIHLDTTHYGALDQFRSWEPILRRK"
     gene            complement(875..951)
                     /locus_tag="VI359_06630"
     tRNA            complement(875..951)
                     /locus_tag="VI359_06630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(915..917),aa:Pro,seq:ggg)
     gene            complement(1044..1394)
                     /locus_tag="VI359_06635"
     CDS             complement(1044..1394)
                     /locus_tag="VI359_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY6973962.1"
                     /translation="MGNEPRLGSKVFYKIGEVSHITKLPAYVLRFWESEFPFLKPKKS
                     RGNQRLYVRRDVETVLEIKRMLYDEGHTLAGVKRYWARRGRTVGQRVRPKELAQRLRG
                     HLQAILKVLESHSE"
     gene            complement(1436..1711)
                     /locus_tag="VI359_06640"
     CDS             complement(1436..1711)
                     /locus_tag="VI359_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY6973963.1"
                     /translation="MRKADIANEIYKQVGISKNEAADIVELVLNMLKSVLKKGESVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            complement(1848..>2150)
                     /locus_tag="VI359_06645"
     CDS             complement(1848..>2150)
                     /locus_tag="VI359_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY6973964.1"
                     /translation="GDVVEHAAAVGAGPVNALDHALRKALEKFYPELREVKLLDYKVR
                     VLSANRGTESKVRVLIESGDHKDKWGTVGVSGNIIEASWQALADSIEYKLLSRDRP"
BASE COUNT          463 a          660 c          532 g          495 t
ORIGIN      
        1 cttccgtcga aggatcggct cccacgatcg aaactgatcc aacgcgccat agtgcgtagt
       61 gtcgagatga atcggcgtca gcgaaacgag accctccgct atggcttcat gatccgcatc
      121 tttgctccga ctccacgaga cacgggtccc ggcaatccag tagtagctcc gtccatgagg
      181 atccaccttt tcgataatgg ggttatcgaa gcggcgtcgg ctcaagcaag tgacgcgaac
      241 cccgcgaacg gctgacaatg gacgatccgg cacattcaca ttcaccagcg tttcatccgg
      301 caggccacgt tcgaggacca ggcgagcaat acggatggca taatctgctc ctacatcgaa
      361 ccgaaatcgt tcatggccct cctgcgaaac cgcgatagag gggattccaa ggatcgtccc
      421 ttccagcgcg gcggagactg ttcccgaata catcacatcg tcaccgaggt tgactccttt
      481 gttgataccc gacacgacca acgcaggcgg tttcggcatg atcttcagca cggccagatt
      541 cacgcaatcc acgggagtcc cgttcacggc atagatgcgc gtgtccattt gctgcactcg
      601 aagcggttta tgaagggtaa ctgcgtgcgc cgccgccgtc cgttctcgat ccggcgcgac
      661 cacccacacc tcaccgatta ccttcaaggt cttggccagc gcggtcagac ccggcgaaag
      721 aattccgtca tcattcgtaa tcaaaatgcg catgaccggc tcgaacacaa aaaaagctgc
      781 ccgtggcagc ttttttcttc aggactaaaa tctgagcacc gagtggctgt gcatcctttt
      841 cttcctagca ctcagccctc agcactttat ttattggtcg gggcgagccg atttgaacgg
      901 ccgaccactc gcaccccaag cgagtacgct accgggctgc gccacgcccc gactctgttt
      961 cccatgcttc gtcgtcgaat gcattttcct cttgcacggc accggaatgc aactcgtgaa
     1021 ctggggatac gacggagtgc cggtcactcg gaatgagatt ccaacacctt gagtatggcc
     1081 tgcaggtgac cgcgaagccg ctgggccaat tctttcggcc gaacccgttg cccgacagtc
     1141 cggccgcgtc gggcccagta ccgcttcacc cctgcgagcg tgtggccctc atcatagagc
     1201 attcgtttga tttctaacac cgtctcgaca tcacgccgga cgtacaatcg ctgattgccg
     1261 cgacttttct tcggcttcaa gaacggaaac tccgactccc agaatcggag gacatacgca
     1321 ggaagcttgg tgatgtgact cacctcaccg atcttgtaaa agaccttact acccagccgg
     1381 ggttcattcc ccatgaccgg tcctcgtggt gggaatagcc ctgtagacga cggagttatg
     1441 aattgacgta cttcttgaac acctgactcg ggcggaaggt gactacgcga cggggagtaa
     1501 tgccaatttc ttcaccggtc cgaggatttc tgccttttct tgcacccttg ctgcgcacta
     1561 caaaattgcc gaaccccgcg atcttcaccg attccccctt ttttaacact gacttgagca
     1621 tgttcagcac gagctcgacg atatcggccg cttcgttttt tgagatgccg acttgcttat
     1681 aaatttcgtt tgcgatgtcc gcctttctca tgctccctcc cctacgtcac ctaactcgaa
     1741 gcattcggca acctgatttc tcgtaccccc agaaggggcc ggtttcgcta taagttacgt
     1801 aaggttatgt ggtctgtcaa ggagaaagat tcgctggggt gaattcctta tggacggtct
     1861 ctggacagca acttgtattc gatgctgtca gccaaggcct gccaacttgc ttcaatgatg
     1921 tttcccgaga ctccaacagt cccccattta tccttgtggt cacccgactc gatcaacacc
     1981 cggactttag attcggtccc acggttggcg gagagcacgc ggaccttata atccaacaac
     2041 ttcacctctc tgagctcggg gtaaaatttc tcaagagcct tccggagcgc gtgatccaag
     2101 gcattgacgg gcccggctcc gacagctgct gcatgctcca cgacatcgcc
//
LOCUS       DATDNW010000276         5025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_454878, whole genome shotgun sequence.
ACCESSION   DATDNW010000276 DATDNW010000000
VERSION     DATDNW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454878"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VI359_06650"
     CDS             <1..757
                     /locus_tag="VI359_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6973965.1"
                     /translation="AMERRQFLKGTLGAASLVALAGCDNLTQSSWFPSILNKAEKLTD
                     RVQRTVTPVNALAKEYHESEVSRVFPANGNVDPGTADYAAHVEQNFAEWTLEIVGLVQ
                     APMSWSLAAIKELPARTQITRHDCVEGWSAIGKWTGVALGDLMHQVRPLPNVRYAVFH
                     CADVDDEGVAYYESMAIADTYHPQTILAYELNGAPLDVPHGAPLRLRFERQLGYKQAK
                     YVMKIELVETLDGIGGGRGGYWEDQGYEWYAGI"
     gene            932..2149
                     /locus_tag="VI359_06655"
     CDS             932..2149
                     /locus_tag="VI359_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973966.1"
                     /translation="MRLKSFSTILIGVVILSGFTGCLSPIAMHRAVIEYDRTVSYVEA
                     DLLLLNIARARYHRPVHFTAVSSVAATFDFRTNAGIRGGIGPSTDSTERAINLEYSAS
                     VAENPTITIVPITGEEFTKRVLRPLDEDKFEFLFRQGYDINMVLRLMARGIAVEEDQG
                     PRVLFNQPSHGDGYVEFRRRLLHLAGLDAERKLFVGPVLFEESHSVRTDRPPNPAEVV
                     AALEKGFRWEGDTEGKVHTVRRKAVGRLLIANYDPNRLGNEERRRLHEEAVRYPPDHV
                     MVDIRPDFPGGDYPLRGSILLRSMNAIIGFVARSIEEEPEMMVSPDSRTKTVVRNPAM
                     TLEIEESSSKPEGYEFSVQFDDRHYSIRKFPVSQGMVPSWNQEAFAVLANLFQMTVTD
                     LTRYPTPAIAIAK"
     gene            2194..2712
                     /locus_tag="VI359_06660"
     CDS             2194..2712
                     /locus_tag="VI359_06660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY6973967.1"
                     /translation="MFSRLTVAFTALLMGFFLTWVMANAGVGMKAPDITNDTWLNSAP
                     LHLPDLRGKVVMVEFWTFGCYNCRNVEPYVKDWYRKYADQGFVVIGVHSPEFSYEHDL
                     ANVKKYIREHDIRFPIPIDNDFSTWNRYGNRYWPAMYLIDKQGTIRYVRIGEGGYQET
                     ERLIQGLLAERS"
     gene            3007..>5025
                     /locus_tag="VI359_06665"
     CDS             3007..>5025
                     /locus_tag="VI359_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEY6973968.1"
                     /translation="MPRPPRPIPSSPEPMNAEQQRLEQDARREQHWKRWGPYLSERAW
                     GTVREDYSPHGTAWEAFPYEHARSRAYRWNEDGLAGICDRHQMICFALALWNGRDPML
                     KERIFGLTGNEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAEFPYARLLEENRRRG
                     SRALEFELIDTGIFEGDRYFDVFVEYAKASPEDVLVQIQVVNRGPDAAELTVLPTVWF
                     RNTWSWGLDVRRPRFRKGEPVKDASVIECDHEYYGCRRLLCQGSPELLFTENETNTKR
                     LYGDAECLRYVKDSINDYIVHGDKGAVNPDQIGSKVSAHYVLNLGPGATSTIRLRFTN
                     HAESVALTGEEHDAVFTQRRREANEFYDGLAPAGLSEDARQVQRQAFAGLLWSKQFYH
                     YDVSRWLKGDPMGPEPPRERLHGRNSDWTHLYNADVISMPDKWEYPWYAAWDLAFHCI
                     PLALVDPTFAKEQLVLMLREWYMHPNGQIPAYEWTFGDVNPPVHAWAAWRVYKIDKKH
                     TGVGDRVFLERVFHKLLLNFTWWVNRKDADGKNIFQGGFLGLDNIGVFDRSAPLPTGG
                     HIEQSDGTAWMAMYTLNMLTIALELARDNRAYEDVASKFFEHFVYICRAINNIGDEKI
                     ELWNKEDGFFYDVLHLPDGQNLPLKVRSMVGLIPLFAVETLESDLIDSL"
BASE COUNT         1117 a         1424 c         1463 g         1021 t
ORIGIN      
        1 cgctatggaa cgccggcaat ttctgaaggg cacgctcgga gccgcaagtc tggtcgcact
       61 cgccggctgc gacaatttga cgcagagcag ttggtttcca tcgatcctca acaaggccga
      121 gaaactgacc gatcgtgtcc aacgaactgt cacaccggtc aacgccctgg cgaaggagta
      181 ccacgaatcg gaggtgtcgc gcgtgttccc ggccaacggc aatgtcgatc cgggtacagc
      241 tgactatgcc gcccacgtcg agcagaattt tgcggagtgg actctagaga ttgtgggatt
      301 ggtccaggcg ccgatgagct ggtcgctggc ggcgatcaag gaactgccgg cgcgaacaca
      361 gattacccgg catgattgtg tcgaaggttg gagcgccatc gggaaatgga ccggcgtcgc
      421 gctcggtgat ttgatgcacc aggtccgacc actccccaac gtccggtatg ccgtctttca
      481 ttgtgccgat gttgatgacg aaggcgtggc ctactacgag agtatggcga tcgccgacac
      541 ctatcatccc caaacgatcc tcgcgtacga gctgaacggc gcaccgttgg atgtcccgca
      601 cggagccccg ttgcgtcttc ggttcgaacg gcagctgggg tacaagcagg cgaagtatgt
      661 catgaagatc gaactggtcg agaccctcga cggcatcggc ggcggacgag gcgggtattg
      721 ggaagaccaa gggtatgaat ggtacgccgg gatttaatca ggatggtgaa acgagccgcc
      781 tgcggcctac cctaaactgt acgcctcggg cgctcagttg ctccggcctt gctcgggaca
      841 aagcgcgtct tggcgcgccg gggcagggcg ggtgacaatg tcgactcgtt tgaccatcct
      901 gcaaattgct ctgaaccgtg aagcgtgacg cgtgagactc aaatcgtttt ccaccatctt
      961 aattggggta gtgattctga gcggtttcac cggttgtctt tcgccgattg ccatgcaccg
     1021 ggcggtgatc gaatatgacc gcacggttag ttacgtcgaa gccgacctct tgctgctgaa
     1081 catcgcccga gcccggtatc accggccggt gcatttcacc gccgtctcca gcgtggcggc
     1141 cacgttcgat ttccggacga acgccggcat tcgcggagga atcggccctt caaccgactc
     1201 cactgaacga gccatcaacc tggagtacag tgcaagtgtc gccgagaatc caacgattac
     1261 gattgtgccg attacgggag aggagtttac caagcgggtg ctgcgccctc tcgatgaaga
     1321 caaattcgaa ttcctcttcc gccaaggcta cgatatcaat atggtcttgc gcctcatggc
     1381 gaggggcata gcagtcgaag aggatcaggg ccctcgcgtg ctgtttaacc agccgtcgca
     1441 tggagacggc tacgtggaat tccgtcgccg tctcctgcat ctcgcaggtt tggacgcaga
     1501 gcgtaaactc ttcgtcggac cggtcctctt tgaagaatcg catagcgtcc gaacggatcg
     1561 gccgccgaac cccgccgaag tcgtcgcggc gctggaaaaa ggctttcgct gggaaggcga
     1621 caccgagggc aaggtccata cagtccgtcg caaagccgtg ggccgactgc tcatcgccaa
     1681 ctatgatccc aatcgtctcg ggaacgaaga gaggcggcgg cttcatgagg aggcagtacg
     1741 gtacccgccg gatcacgtca tggtcgacat ccgacccgac tttccgggcg gcgactaccc
     1801 ccttcgcggc tcgatcctgc tccgcagcat gaatgcgatc atcgggttcg tggcgcggag
     1861 tatcgaagaa gaaccggaaa tgatggtgtc gcccgactct cgaaccaaaa ccgtggtgcg
     1921 caatcccgca atgacactag aaattgaaga atcgtcgtca aagccggagg ggtacgaatt
     1981 ctccgttcag ttcgacgacc ggcactattc gatccggaaa tttcccgtga gtcagggcat
     2041 ggtaccgagt tggaaccagg aagccttcgc ggtcctggcc aacctgttcc aaatgacggt
     2101 gaccgacttg acacggtacc cgacaccggc gattgcgatc gccaagtaga tccgtccggt
     2161 catcctgttc atatcggcaa gagggagggc aacatgttct cgcgtctcac tgtcgctttc
     2221 accgccttat taatggggtt ctttctgaca tgggtcatgg ccaatgcggg agtcggcatg
     2281 aaagcaccgg atatcacgaa cgacacctgg ctgaacagcg ctccgctgca tctgccagac
     2341 ttgcgcggaa aggtcgtgat ggtcgagttc tggaccttcg gctgctacaa ctgccggaac
     2401 gtcgaaccct acgtcaaaga ctggtacagg aaatacgcgg atcagggctt cgtcgtgatc
     2461 ggcgtccact cgccggaatt ctcctacgaa cacgacctcg ccaacgtgaa aaaatatatc
     2521 cgagagcacg acatccggtt tcccattccg atcgacaacg atttctccac ctggaatcga
     2581 tatggcaacc ggtactggcc ggcgatgtat ctgatcgaca agcagggcac cattcgatac
     2641 gtgcgcatcg gcgagggcgg gtatcaggaa accgaacggc tgattcaagg cctcttggcg
     2701 gaacgatcat agactctgcg accgtccctc catccggcat ggcggccaac acgcgcagaa
     2761 tctgtgctga atgtccgcgc gagcccgaag ggcttcctca ccaaacatat gacgccgcga
     2821 tcgacgggag aacaactcgc tcgacgtatt cgccgttaat ccgaaccgaa cggtgcggcc
     2881 gtcgcatgat gtaaagaagg acggaatgat gatgggaagc ttgcaaccac aagaatcaca
     2941 cactagcatt gtaagaatga ggctggttcg ccgtctattc agcaagacat ttcattgagg
     3001 agtcatatgc ctcgtccacc ccgaccgatc ccgtcgagcc cggaaccgat gaatgccgag
     3061 caacaacgtc tcgaacaaga cgcccgcaga gagcaacatt ggaaacggtg ggggccttat
     3121 ctcagcgaac gagcctgggg cacggtacga gaagactata gcccgcatgg cacagcctgg
     3181 gaggcctttc cttacgagca cgcccgctcg cgcgcctatc gctggaacga ggacggtttg
     3241 gcgggcatct gcgatcgcca ccagatgatc tgtttcgcgc tcgcgttatg gaatggacga
     3301 gaccctatgc tcaaggaacg gatcttcggc ctgaccggga acgaaggcaa ccacggcgaa
     3361 gacgtcaaag agtattactt ttatctggat tccacaccga cccattccta catgaagtat
     3421 ctctacaagt acccgcaggc cgagttccca tacgcgcgcc tgctcgagga aaatcggcgg
     3481 cgtgggtcgc gcgctttgga atttgagctg atcgatacag gtatcttcga gggagaccgc
     3541 tacttcgatg tgtttgtgga atacgcgaaa gcctctccgg aggatgtgct cgtccagatc
     3601 caggtcgtca atcgcgggcc ggatgctgcg gaactgaccg tgctcccgac agtctggttc
     3661 cgcaatacct ggtcgtgggg gcttgatgtt cgccggcctc gattccgcaa aggagagcct
     3721 gtcaaggatg ccagtgtcat cgaatgcgac catgagtact acggctgccg acgcctcctc
     3781 tgtcagggca gtccggagtt gctcttcacc gaaaatgaga cgaatacgaa gcggctttat
     3841 ggagatgcag agtgtttgcg ctatgtgaag gacagtatca acgactacat cgtgcacgga
     3901 gacaagggcg cggtcaatcc ggatcagatc ggttcaaagg tgtccgccca ctacgtgctg
     3961 aatttggggc ctggcgcgac atcgacgatc cgattgcggt ttaccaatca tgccgagagt
     4021 gttgccctga cgggagagga gcatgacgcg gtcttcaccc aacgtcggcg agaggcgaat
     4081 gaattttacg atggtctggc acctgccggt ttatccgaag acgccagaca ggtgcaacgc
     4141 caggcattcg cgggcctact ttggagcaag cagttctacc actacgacgt gagtcgctgg
     4201 ctgaagggcg atccgatggg tcccgagccg ccgcgcgaac ggctccacgg ccgcaactcc
     4261 gactggaccc acctctacaa cgccgacgtc atctcgatgc cggacaaatg ggaatatccc
     4321 tggtatgcgg cgtgggactt ggcatttcat tgcatcccgc ttgcgttggt tgatccgacc
     4381 tttgccaaag agcagctcgt cctgatgctc cgcgaatggt acatgcaccc caacgggcaa
     4441 attccggcat acgaatggac cttcggcgac gtgaaccctc cggtccatgc ctgggcagcg
     4501 tggcgcgtct ataagatcga caagaaacat acaggtgtcg gcgaccgtgt cttcttggaa
     4561 cgggtctttc acaaactgct gttaaatttc acctggtggg tgaaccgcaa agatgcagat
     4621 ggaaagaata tcttccaggg cggctttctc ggtctcgaca acatcggcgt ctttgatcgc
     4681 agcgcgccgc ttcccactgg tggccatatt gaacagtccg atgggacagc ctggatggcc
     4741 atgtacacac tcaacatgct gacaatcgcg ctcgaacttg cacgcgacaa ccgcgcctat
     4801 gaagatgtcg ccagcaagtt tttcgagcat ttcgtctata tctgccgggc gattaacaac
     4861 atcggagatg aaaagatcga gctttggaat aaggaagatg gcttctttta cgatgtcctg
     4921 catctgcctg atgggcagaa tctccccttg aaggtgcgct ccatggtcgg attgatcccg
     4981 ctcttcgcgg tggagacgtt ggagtcagac ctgatcgata gcctg
//
LOCUS       DATDNW010000277         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_922726, whole genome shotgun sequence.
ACCESSION   DATDNW010000277 DATDNW010000000
VERSION     DATDNW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922726"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1706)
                     /locus_tag="VI359_06670"
     CDS             complement(318..1706)
                     /locus_tag="VI359_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6973969.1"
                     /translation="MRATIFVTDDEPAIRAAIIKRLSRRHHRVVGYDSGDALMKAIEH
                     DLPDLVLLDLKMPGLSGLDTLKQLRPLVPHALVIMLTAYGTVQDAVEAMKLGAYDFLI
                     KTVDLEGIDPVVDRALEFLALRRRIDFEQEHESSQYALSNLDARSTTMKQLLSQVRDV
                     ADNPKSTVLLQGETGTGKEFLARVLHHNGPRSSGPFIGVNCTAIPKELFESELFGYER
                     GAFTGAHQRKVGLLEKAEGGTLFLDEIGDLDLSMQGKLLRVLQERSFRRLGGTDDISV
                     DFRLMTATNRDLKKEVTRGTFREDLYFRLNVVSFELPPLRTRVEDILPLSMSAIVRFG
                     KEFGKEVLDIEPEAKALLERYSYPGNIRELQNIIERAMILCHGKTLTVSHLPRELREA
                     SLQIAVASTQGDQPIVRAEMQLGKQSLADMEFAIIEEVVRLSGHNKSLAAKHLGITRF
                     ALDRRLKKIADA"
     gene            complement(1776..>2481)
                     /locus_tag="VI359_06675"
     CDS             complement(1776..>2481)
                     /locus_tag="VI359_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6973970.1"
                     /translation="QVVMYQGFNIDITALDQMQTRLSRSERLATLGQVAAGIAHEIRN
                     PLVGIGSTTSLLVDDTDLSDPRRSDLEVILRETRRLDRIVNQIIEYARPRELAPVLFS
                     FADLVQEVLKLLDASLSAKALKVTCAIPPTLPQVHADRDQMKQVLLNVFQNAIEASGE
                     GTGLSVAAGPFARNLDPGIVITVTDQGPGISSEDLPHVFEPFFTRGKHRGTGLGLAIC
                     RNIVDAHGGDIQLTSG"
BASE COUNT          536 a          724 c          672 g          549 t
ORIGIN      
        1 tggcgtacgg ctcgtatcag cgttccctac gcgggaaatg gtgttgcccg tggagatctg
       61 gctcgcgtga ccgataagac gcccgcctct agttcggcgg gccattcagg tccggtggac
      121 tgaggtcttc catcattgac ctcctccgtg gtgaacaaac gaacacggag aatctccgac
      181 agatgacata ctccaggctt tcatacgcct cgcaacgtcc tctgtcaact ggcggaactc
      241 cttgcaatca cggcccaacg tggtgaggac aacatcccgc acaaacctcg cgaatgcctt
      301 cgttctccga aacaggttca cgcatctgca atctttttca aacgccgatc taatgcgaaa
      361 cgggtgatgc ccagatgctt ggcggcaagg ctcttgttat gacctgaaag gcggacgact
      421 tcttcaatga tcgcgaattc catgtcggcc agtgactgtt tgccgagctg catttccgct
      481 cgcacgatgg gttgatcccc ctgtgtggaa gccaccgcga tttgcagaga cgcctctcgc
      541 aattcgcggg gcaggtggct gaccgtgagt gtcttgccgt ggcagagaat catcgcccgt
      601 tcgatgatgt tctgaagctc acgaatgttt cccggatagg agtaccgctc gagaagcgct
      661 ttggcttcgg gttcgatgtc gagcacctct ttcccgaact ctttgccgaa tcggacgatc
      721 gcgctcatag aaagggggag aatatcttcc actcgggttc gaagtggtgg cagttcgaag
      781 gagacgacgt tcagccgaaa gtagagatct tcgcggaagg ttccccgcgt cacctccttc
      841 ttgagatcgc ggttcgttgc cgtcatgagc cgaaaatcca ctgagatatc gtccgttccg
      901 cccaaccgtc ggaacgacct ttcttggagc acacgaagca gttttccctg catggacaaa
      961 tccagatcgc cgatctcgtc gagaaacagt gtcccgccct cggctttctc caacaatccg
     1021 acttttcgtt gatgcgcgcc ggtaaaggca ccgcgttcat agccgaatag ctcgctttca
     1081 aatagctcct tggggatggc cgtgcaattg acgccgatga aggggccgga ggaccggggg
     1141 ccgttgtgat gcagcacccg tgcgagaaat tctttcccgg ttccggtttc tccctggagc
     1201 aagacggtag acttgggatt gtccgccacg tcgcgaactt gagacaacaa ttgtttcatc
     1261 gttgtgcttc gcgcgtccaa attcgagagc gcatactgac tgctctcatg ctcctgctca
     1321 aaatcgatac gtcgtcggag cgcgaggaat tcaagcgctc gatccacaac cggatcgatg
     1381 ccttcaagat ccaccgtttt gatcaagaag tcgtacgcac cgagcttcat cgcttcgact
     1441 gcatcctgta cagtcccgta cgccgtcaac ataatcacca acgcgtgggg gaccagggga
     1501 cgcagctgtt tcaacgtatc cagaccgctg agaccaggca ttttcagatc cagcaatacc
     1561 aagtcaggaa ggtcgtgctc gatcgccttc atgagggcgt ctcccgagtc ataccccacg
     1621 acacggtggt gccgtcgtga caagcgtttg ataattgcag cacggatcgc cggctcatcg
     1681 tcagtgacaa agatcgttgc gcgcatacgt taaacctcga tcagtctggg ctgttgtcga
     1741 agagggagcc agagccgagc cgtcgtcccc ttccctcaac cgctcgtcaa ttggatgtcg
     1801 ccgccgtggg catcgacgat atttcggcaa atggccagac cgagtccggt ccctcggtgc
     1861 ttcccccgtg tgaagaaagg ttcgaacacg tgtgggaggt cttccgacga tattccaggt
     1921 ccttgatccg tcacggtaat cacaatgccg gggtccaagt ttctcgcgaa tggacctgcc
     1981 gcaacgctga gcccggtgcc ctccccagaa gcctcaatcg cgttctgaaa tacattcaac
     2041 aacacctgtt tcatctgatc tcgatcggca tgaacttgag gcaaagtggg agggatggca
     2101 caggtaacct tcaacgcttt cgctgaaaga gaggcgtcga gcagcttcaa cacttcctgc
     2161 accagatcgg cgaaggagaa gagcaccggg gccaactcgc gcggcctcgc atactcgatg
     2221 atctgattca cgatccgatc caggcgacgc gtctctcgca aaatcacctc aagatcagaa
     2281 cgtcgagggt cactcaggtc ggtgtcgtcg accagcagcg acgtggtcga cccgatcccg
     2341 acgagcgggt tccgaatttc atgggcgatc ccggccgcga cctgaccgag ggtggcgagc
     2401 cgctctgatc gactcaatcg tgtttgcatt tgatccagcg cagtaatatc gatattgaac
     2461 ccttgataca tgaccacctg t
//
LOCUS       DATDNW010000278         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_381043, whole genome shotgun sequence.
ACCESSION   DATDNW010000278 DATDNW010000000
VERSION     DATDNW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381043"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VI359_06680"
     CDS             <1..840
                     /locus_tag="VI359_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY6973971.1"
                     /translation="DLRGRTEILKVHTKKVPVAMDVELEKIARGTPGFSGADLENLVN
                     EAALWAARQNKKEVELVDFEMAKDKVLMGAERKSMILSDEEKRITAYHEAGHALMAKL
                     LPGTDPVHKVTIIPRGRALGVTMQLPTDDRHNYSKEFLYNTLAILMGGRVAEELVLKN
                     ITTGAGNDLERATDLARKMVCEWGMSEKLGPLTFGRKEEEIFLGREIATKRDFSEQVA
                     LDIDNEIRRLVSENYERAKRLLTEHMRSLKALAEALLEKEVLDAPEIDQILMQSTSQT
                     VPA"
     gene            1046..1909
                     /gene="folP"
                     /locus_tag="VI359_06685"
     CDS             1046..1909
                     /gene="folP"
                     /locus_tag="VI359_06685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY6973972.1"
                     /translation="MIVRTKARTIEINDRPLVMGVVNVTPDSFFDGGRYATPDAAIRH
                     ALRLVEEGADLLDIGAESTRPGSDSIDEGEERRRLMPVVTAVAKAVSIPISVDTMKAT
                     VARAALEAGAVIVNDVSALQADPAMAEVVATTGAGVVLMHMQGSPRTMQEAPVYDDVV
                     GEVAEFFRERMRFCRDRGIEQDQIVLDPGIGFGKLLLHNLTLLGQLETFVSFGRPILV
                     GVSMKSFIGQVVGRPVEERSWGTAAAIALAVSKGAGILRVHDVKAMKEVVAMAAAITR
                     YVGATVRVQHA"
     gene            1902..3251
                     /gene="glmM"
                     /locus_tag="VI359_06690"
     CDS             1902..3251
                     /gene="glmM"
                     /locus_tag="VI359_06690"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY6973973.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSETAMQLGRAAAHLFMRRAGRHQI
                     VIGKDTRISGYMLESALTSGICSMGVDVLLVGPMPTPAVAFLTRSLRADAGVMISASH
                     NPYQDNGIKFFSNDGLKLPDDMEARIEQLIVSNEISHLRPTADAIGKAFRIDDAEGRY
                     IEFVKRSVPKDLDFQGMKLVVDCAHGAAYKVAPKVLRELGAKVEAIGVQPDGMNINAG
                     CGAVHPEVLQEAVREHGAHLGIALDGDADRAIFVCEKGQVIDGDHIMAALGLDLHRHG
                     QLAKQTVVGTVMSNFGLEMALSKAGITLARTAVGDRYLLERMLGDGFNFGGEQSGHFI
                     FLDHNTTGDGLISSLQVLSLMKRTQKPLSELAQAMTAVPQVLVNVKVSHKPKLETVPD
                     LEEAIRAGERRLNGSGRVLVRYSGTESLLRIMVEGERDALIHEVADQLAQVVRTHLG"
     gene            3281..>4054
                     /locus_tag="VI359_06695"
     CDS             3281..>4054
                     /locus_tag="VI359_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY6973974.1"
                     /translation="MLPSLALAFIAGLLLGSQVPYFPLSVSFALLVVALIGVALERST
                     LIPARLVSWCYGVLLLGVVYWTIVVGTTVHDPGTARLDEGFLNLSGRIVEPVQQSADR
                     FLIIVKLDESASESWRSQRVRLTWRAPERQVFHGDRIAFRARLRPPTGSLNPGGFDYA
                     AYLERQGIDAVATVTGADAIQVLQSGRTDGWWTVWNQIDRWRGRIRDAAVKTLSQPAL
                     GLYLGIVIGDRGYLDGSLRDQFMVTGTVHLLSISGSHLGL"
BASE COUNT          863 a         1030 c         1272 g          889 t
ORIGIN      
        1 gatttgcgcg ggcgaactga aattctcaaa gttcacacga agaaagtgcc ggttgcgatg
       61 gacgttgaac tggagaagat tgctcgtggc acgccgggat tttccggtgc cgatttagag
      121 aacctcgtga acgaagcggc cttgtgggca gcccgccaga acaagaagga agtcgaactg
      181 gtcgactttg agatggctaa ggacaaagtg ctcatggggg ctgagaggaa gagcatgatt
      241 ctgagcgatg aggaaaagcg gattacggcc tatcatgaag caggccatgc tctcatggct
      301 aagttgcttc ccgggacgga ccctgtccat aaagtcacga tcattccccg tgggcgtgcg
      361 ttgggcgtca ccatgcagtt gcccacagat gatcggcaca actactcgaa ggaatttctt
      421 tacaatacgc tggccattct catgggcgga cgagttgcgg aagaactcgt cttaaagaac
      481 atcaccaccg gagccggaaa cgatctggaa cgcgcgaccg atctggcgag aaaaatggtg
      541 tgtgagtggg ggatgagcga gaaattggga cccctcacgt tcggccgaaa agaggaggag
      601 attttcctgg gtcgagaaat tgcgacaaag cgcgatttca gcgagcaggt tgcgttggat
      661 atcgacaacg aaatcaggcg gcttgtgagt gagaattacg aacgagccaa gcggctcctc
      721 actgagcaca tgcgcagtct taaagcattg gcagaagcac ttttggaaaa ggaagtgctt
      781 gatgcgccgg agattgatca gattctcatg cagtccactt ctcagaccgt acctgcctaa
      841 tcaccgcatt cggtcacccc gcggctcctt ttcgaggcac aggagtcagg ggagacacac
      901 agagcttctg ttcctgtttg gaaggcaatc cttcgcgggg ttgtgtgtat ctcttcggga
      961 cctgacgcga agacaacgag ttgaaaagcc attatgcagt cataggatga cctggaagcg
     1021 tgtgtttgcg ggaggctgag gattcatgat tgtgcgaacc aaagctcgaa cgattgagat
     1081 caacgatcgt cccttagtga tgggagtggt caatgtcacc cctgactcat ttttcgacgg
     1141 agggcggtac gcgacgccgg atgccgcgat ccgccatgcg cttcgacttg tggaggaggg
     1201 ggcggacttg ctcgacatcg gagctgaatc cactcgtccc ggctcggact cgatcgatga
     1261 aggagaagaa cgccgacgcc tgatgcctgt ggtgacggcc gtggcaaagg cggtgagcat
     1321 tccaatctcg gtcgatacga tgaaagcgac ggtggcacgg gctgcattgg aagcgggagc
     1381 ggtcatcgtc aatgacgtga gcgcactgca ggctgatccg gccatggcgg aggtggtggc
     1441 gacgacaggg gcgggagtcg tactcatgca catgcaaggg agccccagaa cgatgcagga
     1501 agcgccggtg tatgacgatg tcgtcggcga ggtggcggag tttttccggg aacgaatgag
     1561 attttgcagg gatcgaggga ttgaacaaga ccagatcgtg cttgatccag ggataggatt
     1621 tggtaagctg ctgctacata acctgacgtt gctagggcag cttgagacct tcgtgtcgtt
     1681 cggtcggccg attcttgtgg gagtatccat gaaatcgttt atcgggcagg tcgtggggcg
     1741 cccagtggaa gaacggtcct ggggaacggc cgcggcgatc gccctcgcag tcagcaaagg
     1801 agccggaatc cttcgagtcc atgacgtcaa agccatgaaa gaggtggtcg ccatggccgc
     1861 ggcgatcaca cggtacgtcg gtgcaactgt gagagtccaa catgcgtaag ttattcggaa
     1921 cggatggagt ccgaggagtc gcgaatctcg aacccatgac gagtgaaacg gcgatgcagc
     1981 tcggccgagc tgctgcccat ctcttcatgc gacgggccgg gcgccatcag atcgtgatcg
     2041 gcaaagatac ccgcatctcc gggtacatgt tggagtccgc gttgacctcc ggtatctgtt
     2101 cgatgggagt ggatgtcctg ctggtcggtc cgatgccgac ccccgctgtg gccttcctca
     2161 ccagaagcct cagggctgat gctggtgtga tgatttcggc gtcgcacaat ccctatcaag
     2221 acaacggaat caaattcttt tcgaatgacg gcttgaagct gcccgatgac atggaggcgc
     2281 gcatcgagca actcatcgta tcgaatgaga tcagccattt gcggccaaca gccgatgcga
     2341 ttggaaaagc atttcgtatc gacgacgccg aaggacgcta catcgagttc gtcaagcggt
     2401 cggtgcccaa ggatctggac tttcagggta tgaaactcgt cgtcgattgt gcccacggag
     2461 cggcgtataa ggtcgcgcca aaagtgctgc gggaattagg ggccaaggtc gaagcgatcg
     2521 gcgttcagcc ggatgggatg aatatcaatg cgggctgtgg ggcagtccat cctgaggtct
     2581 tgcaggaggc ggtgcgtgag catggggccc atctcgggat cgcgctcgac ggtgatgccg
     2641 atcgtgccat atttgtgtgc gagaagggac aggtgattga cggagatcat atcatggcgg
     2701 ccctggggct tgatctccat cgacatggcc agcttgccaa gcagaccgtc gtgggtaccg
     2761 tcatgagtaa ttttggcctt gaaatggctc tctcaaaagc cgggattacg ttggcccgaa
     2821 ctgcggtggg agaccgctat cttctcgagc ggatgttggg tgatggtttc aacttcggcg
     2881 gggagcagtc cgggcatttt atttttctcg atcacaacac gaccggcgac gggttgattt
     2941 cttccttaca agtactgtcg ttgatgaagc ggacgcaaaa accgctgtcc gagttggccc
     3001 aggccatgac ggcggttcca caagtcctgg tcaacgtcaa agtctctcat aagcccaagc
     3061 ttgaaacagt tcctgatctt gaggaggcca tccgcgcagg cgaacgtcgc ctgaacggga
     3121 gcgggcgggt attggttcgg tattcgggaa ccgaatcctt gttacgcatc atggtcgagg
     3181 gcgaacgcga tgcccttatt cacgaagtcg cggatcagtt ggctcaggtc gttcgaaccc
     3241 atctaggata gtcgtctctc ccctcgagtg agggaggcca atgctgccgt ccctcgccct
     3301 tgcctttatc gccggacttc tcctcggatc gcaggttccc tatttcccac tatccgtctc
     3361 cttcgccctg cttgtcgttg ctcttattgg tgttgccctc gaacgttcca cgttgattcc
     3421 ggcccgcctc gtgtcgtggt gctatggtgt cctgttgtta ggtgtcgtct attggacgat
     3481 cgtggtcggg accacggttc atgatcccgg tacagcacgg ctcgacgagg gctttctcaa
     3541 cctgagtggg cggattgtcg agcccgtgca acaatcagct gatcgctttc tgataatcgt
     3601 gaagttggac gagtcggctt ctgaatcgtg gcgttcacaa cgtgtcagac tgacatggcg
     3661 cgcgccggag cggcaggtct tccacggcga tcggattgcg ttccgcgcgc gtcttcgccc
     3721 gccaacaggc tcattgaatc ctggcggatt tgattacgcg gcgtatctcg aacgccaggg
     3781 tattgatgcg gtggccacgg tgacgggagc ggatgcgatc caggttctgc aatcgggccg
     3841 taccgacgga tggtggacag tctggaatca gattgatcgc tggcgagggc gcatccgaga
     3901 cgctgcagtc aaaactctct cccagccggc tcttgggtta tacctgggca tcgtcatcgg
     3961 agatcgtggc tatcttgacg gatcgctccg cgaccaattc atggtgacag gaacggtcca
     4021 cctgctctcg atttccggat cgcacttggg cctg
//
LOCUS       DATDNW010000279         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_381815, whole genome shotgun sequence.
ACCESSION   DATDNW010000279 DATDNW010000000
VERSION     DATDNW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381815"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /gene="moaC"
                     /locus_tag="VI359_06700"
     CDS             complement(<1..390)
                     /gene="moaC"
                     /locus_tag="VI359_06700"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY6973975.1"
                     /translation="MAEFTHFNESGRARMVDVSAKDATERVATAQAKVFLQPETLEKI
                     RRGKIAKGDVLSVAQVAGVMGAKKTPDLIPMCHPILLTSVDISFKEEPQPDREGRCSI
                     TITATAKTTGPTGVEMEALTAASVAALT"
     gene            522..1472
                     /locus_tag="VI359_06705"
     CDS             522..1472
                     /locus_tag="VI359_06705"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY6973976.1"
                     /translation="MTRRIIIERLEFQGCCGVTPEERRRPQPLAIDLELECQAEPGHV
                     SDNIQEVVDYAQVTERIVELGTTQGCALLETFAEHILTMLFTEFPVDRVNLWLRKLAP
                     PLLYATGSVGIQVDRPRMAHPVQSSEPTPARFLTQQLHRLPKGRALDVAAGSGRNALY
                     LASHGFQVDALDRDEQRLIALAALAKQRNLPSLGVHTVDLERPTDDRPEFPKNTYDVI
                     VVFFYLHRPLFPWLMEALKPNGVLIYETFTIDNYFRHHHPRRWEFCLAQNELLRLTSD
                     LRVLSYDEGEHDGGHGLVPAYTAQLVAKKSGLVERSHGPH"
     gene            1459..2307
                     /locus_tag="VI359_06710"
     CDS             1459..2307
                     /locus_tag="VI359_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY6973977.1"
                     /translation="MGRIDLHLHTTHSDGSLRPAEVLTLAQKAGVTALAITDHDIMDA
                     LPEAIEAGAQLGIEVLPGVEISSFDGKGELHILGYCLNWRDETLQSRLALLRASRHRR
                     NPLIIERLQALGLELTYDEVRALAGTESVGRPHIARLLMEKGYVTSAKEAFDRYLAEG
                     RPGYVARELPTPAEAISWIRDAQGVAVLAHPTWVKESDNALPEFVRVLKDTGLGGIEV
                     HYSTHTQQQTTRFLELAKQFGLLVTGGSDFHGLTKPDIEVGAGRGSLKVPEQLLAPLK
                     VAAARA"
     gene            2426..>2762
                     /locus_tag="VI359_06715"
     CDS             2426..>2762
                     /locus_tag="VI359_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973978.1"
                     /translation="MSTRWNWIGPYSFAILAALVAGPLLSSLSLVQSLSIVSLQLSGP
                     NVVRLAADGLALILLWLMAAAAFREIPDNGRGLGFFRSLILPITALLTVIFTNKAMKT
                     VGLPFVEQIG"
BASE COUNT          574 a          844 c          739 g          605 t
ORIGIN      
        1 cgtgagagcg gccacagagg cggcggtcag tgcttccatt tccacaccgg tcggtccggt
       61 tgtcttggct gtcgccgtga tcgtaatcga gcaacggcct tctcgatccg gctgcggttc
      121 ttctttgaag gagatatcca cgctcgtgag gagaatggga tgacacatgg gaatgaggtc
      181 cggggtcttt tttgccccca tgactccggc cacctgggcg accgacagga cgtcgccttt
      241 tgcaatcttt ccgcgccgga tcttttcgag agtttccggc tggagaaaaa ccttcgcctg
      301 cgccgttgca acccgctcgg tcgcatcctt cgcgctgaca tcgaccatcc gggcacgacc
      361 cgactcgttg aagtgcgtaa actctgccat tttcctcgat gaatcaggca gttgcaaaga
      421 aactcgatga gattataggt ggccctcaaa caacgagtca agctcgactc ctcctaaaga
      481 gttccttgac agttgaaatt tcaatcctgg acaatgccga catgacccgt cggatcatca
      541 tcgaacggct tgaatttcaa gggtgttgtg gagtgacacc ggaagagcgc cgtcgcccgc
      601 aaccgctcgc tatcgatttg gagctggagt gccaggccga accggggcat gtgtcagaca
      661 acattcagga agtcgtcgat tatgcgcaag ttaccgaacg gatcgttgaa ttgggcacga
      721 cgcaaggatg tgcactcctt gaaacattcg ccgaacacat cctgacgatg ttgttcaccg
      781 agttccccgt tgatcgcgtg aatctctggc tcaggaaact cgctcccccg ctcctctacg
      841 ccaccggatc tgtgggcata caggttgacc gaccccgcat ggcgcatccc gttcaatcct
      901 ccgagccgac gccggcaaga ttcctcaccc agcaactgca tcggttgccg aagggccggg
      961 cactcgatgt cgcagcaggc tccggccgca atgctctgta tttggcttca catgggttcc
     1021 aagtcgatgc gctcgaccgc gatgaacaac gattgattgc tctcgcggca ctggcaaaac
     1081 aacggaacct tccgagcttg ggcgtgcata cggtcgatct cgagcgtcct accgacgacc
     1141 gacctgaatt tcccaagaac acctatgatg tgatcgtggt attcttctat cttcatcggc
     1201 cactcttccc atggctgatg gaggcgttga aaccgaatgg cgtcctgatc tacgaaacgt
     1261 ttaccatcga taattatttc cggcatcacc atcctcggcg atgggagttc tgcttggcac
     1321 agaacgaact cctccggctc acgtcggacc ttcgtgtcct ctcctacgat gaaggtgagc
     1381 acgacggggg tcacggtctt gttcctgcct acacggcaca actcgttgcg aaaaaatccg
     1441 gacttgtaga gcggtctcat gggccgcatt gacctccatc tccataccac ccactctgac
     1501 ggaagcctgc gaccagccga ggtcctcact ctcgcccaga aagccggagt caccgcgctt
     1561 gccattacgg accacgacat catggacgct ctccccgaag cgatcgaggc aggggctcaa
     1621 ctcggaatcg aggtacttcc cggcgtggaa atcagctcct tcgacggcaa aggtgagctc
     1681 catatcctgg ggtattgcct caactggcgc gatgagacgc tccaaagccg tttggccctg
     1741 ctgcgtgcca gccgacatcg ccgcaaccca ctcatcattg aacgactcca ggcattgggg
     1801 ctggaactca cctatgacga agtcagagcc ttggcaggaa cagaatctgt cggtcgaccg
     1861 catatcgctc gactcttgat ggaaaaggga tatgtcacct ccgccaaaga agctttcgac
     1921 cgatacctcg cagaaggacg accagggtat gtggcacgcg agttaccgac cccggctgag
     1981 gccatcagct ggatcagaga tgcacagggc gtggctgtct tggcccatcc tacctgggtg
     2041 aaagaatctg acaacgcatt gccggagttc gttcgggtcc tcaaggacac tgggctcggc
     2101 ggcatcgaag ttcattacag cacccacacg caacagcaga ccacgcggtt tctcgaactt
     2161 gccaaacagt tcggcctctt ggtcacagga ggaagcgact tccatggact gacgaagcct
     2221 gacatcgagg taggggctgg tcgtggaagt ctgaaagttc cagagcagtt gctcgctccg
     2281 ttgaaggtag cggcagcgcg ggcgtaacgt ccgttccccc cactgcttac tgttcctgaa
     2341 accctcgcgc gttttcgcct attcgttgac tctctgttgc catccgctag actttgacac
     2401 atccatcgga agcctgacca cgatcatgtc gacacgctgg aattggattg gaccgtacag
     2461 ttttgccatc ttagcggcgc tcgtcgccgg cccccttctc agcagtctct cgctcgtcca
     2521 gtcgctgtcg atcgtctctc tgcaactaag cggccccaac gtcgtccggt tggccgccga
     2581 tggcctggcc cttatcctcc tttggctgat ggctgccgcc gccttccgag agatcccgga
     2641 taacggccgc gggttgggtt ttttccgcag cctcatcctc ccaatcacgg cccttctgac
     2701 ggtgatcttc accaacaaag ccatgaagac cgtagggctc ccatttgtgg aacagatcgg
     2761 ga
//
LOCUS       DATDNW010000280         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_245435, whole genome shotgun sequence.
ACCESSION   DATDNW010000280 DATDNW010000000
VERSION     DATDNW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245435"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..89
                     /locus_tag="VI359_06720"
     CDS             3..89
                     /locus_tag="VI359_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973979.1"
                     /translation="MESLLAIKRAGADVILTYFAKDAARLLH"
     gene            95..1045
                     /locus_tag="VI359_06725"
     CDS             95..1045
                     /locus_tag="VI359_06725"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY6973980.1"
                     /translation="MLKVTRGLSDAGPRAHPVATIGNFDGHHLGHRMLLQTVVEAARK
                     AQGTALVLTFEPHPVKVLAPHVELRYLTSSEEKLDRFEAAGIDEVVLLEFTPTFASMT
                     PEQFADRVLHQQLGLAELFVGEHFAFGKGRAGRIADLIRFGGQFGFQVHPVRPVMVEG
                     AVVSSTRVRQLIQAGEMERAAMLLGRAYGIQGTVIHGAQQGQALGWPTANLRLPPHRV
                     MPPDGVYAARTVYGGKTFEAIAYIGTRPTFGEGERLIEVYLLDQSCNLYGEEITVQFV
                     ARVRGDHAFASPDELSKQIARDVEQARLSLQRLPEGMREA"
     gene            1054..2532
                     /gene="tilS"
                     /locus_tag="VI359_06730"
     CDS             1054..2532
                     /gene="tilS"
                     /locus_tag="VI359_06730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY6973981.1"
                     /translation="MTISKEASKPAARPALLNEMVRTVRRQALFLPGQHLLVAVSGGA
                     DSMALLSLLHRLAPSWRLSLTAVHFNYGLRGGESQGDESFVRTWCGEWRLPLIVQRPV
                     LPKNRRASSLQAVAREVRYTAMQQMASDLGAERIVVGHTANDQAETMVMWMLRGAGLR
                     GLAGMPYMREGIVVRPLLASTREDILTYLDQQGVPYRSDSSNDKPLYHRNRIRREIMP
                     ALTRLRPAAVRVLQRHADLLRDDDAYLEQTVEGLMSSFVSNDEEGRQRLDRQGISILP
                     VALQRRLIRQILRRCDEQKRAPGVRLVEAVRQFLLKGQPGDRLMVRSGMLIQKHDCTE
                     LSRSRNGCLTGAPDADRDSTAACCLAIPSVACWGRRNFQFQVQLMTRDEAAQLPPARW
                     AHRALFDADRFSTPLVVRAWQAGDRFCPTGMRGRTKKLQDLFTDLKIRREERGDIPLL
                     VASEGILWVVGKRQDDRFAVRNSTTRCLVVTVMARSEREGAL"
     gene            2534..3073
                     /gene="hpt"
                     /locus_tag="VI359_06735"
     CDS             2534..3073
                     /gene="hpt"
                     /locus_tag="VI359_06735"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY6973982.1"
                     /translation="MERLFGRPIVTQEEMRTRIRELGKQITADYSGKDFVLVGVLKGA
                     YAFYADLARAIRIPMRVDFIVVTSYGSQAKTSGKVKMVTELTEDIKGKDVLLVEDIVD
                     SGLTIQYLVKQLSKRKPRSVKICTLLSKPERRTVDVPLAYIGFNIPDKYVVGYGLDYQ
                     QKYRNLPYLAVLDVDGKQE"
     gene            3176..>3988
                     /locus_tag="VI359_06740"
     CDS             3176..>3988
                     /locus_tag="VI359_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY6973983.1"
                     /translation="MNSRAKNLLFWVVVGLFMILLFNLFSVPTHAPEEEVIFSDFMAK
                     LDKGDIEKVIIKSSHISAILKDKTRIRTYSAEYPELVKVLREKGVQIEAKPPDESPWY
                     ITFLVTWGPFVLFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGID
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHD"
BASE COUNT          870 a          992 c         1227 g          899 t
ORIGIN      
        1 tgatggaatc cttgttagcg atcaaacggg ccggtgcgga tgtgatcctc acctactttg
       61 ccaaagacgc ggcccggctt ctacactgac cacgatgctg aaagtgacgc gtgggctctc
      121 cgatgccggt ccacgtgccc atcctgtcgc aaccattggc aattttgacg gccaccatct
      181 cggccaccgg atgttgctgc agactgtggt agaggcggct cgaaaagcgc aggggacggc
      241 gctcgtgctg acgttcgagc cccatccggt gaaagtgctc gctccgcacg tcgaacttcg
      301 ctatctgacc agttcggagg aaaaactgga tcgttttgag gctgccggga tcgatgaagt
      361 cgtcttgctg gaatttacgc cgacctttgc ctcgatgaca cccgagcaat tcgccgatcg
      421 cgttctgcac cagcagctcg ggttggcaga actctttgtc ggcgagcatt ttgcgtttgg
      481 gaaggggaga gccggtcgaa tcgccgatct gattcgattc ggcggccagt tcggattcca
      541 ggtccatcct gtccgaccgg tcatggttga aggagccgtg gtgagttcga cacgggttcg
      601 ccagctgatt caagcaggag agatggagcg agctgcgatg ctgttggggc gcgcctatgg
      661 aattcaaggc acggttattc acggagccca gcagggacaa gctttaggat ggccgaccgc
      721 caatctccga cttcctcccc atcgggtgat gccgcctgat ggggtctatg ccgcacgaac
      781 tgtctatggc ggaaagacgt ttgaggcgat tgcgtatatt gggacaaggc cgacatttgg
      841 agagggagag cgtttgattg aggtgtattt actggatcag tcgtgcaacc tctatggtga
      901 agaaattacc gttcagtttg tcgcgcgggt acggggtgac catgcgttcg cttcccccga
      961 cgaattgtcg aagcaaattg cccgcgatgt cgaacaggca cggctcagtc tgcagcgtct
     1021 tcctgaagga atgcgggaag catgatgatg gagatgacta ttagcaaaga ggcatccaag
     1081 cccgctgctc gacccgcgct tctgaatgaa atggtcagaa ccgtcaggcg ccaggcgttg
     1141 ttcttgccgg ggcagcatct tctcgtggct gtatcgggag gagctgattc aatggcgctg
     1201 ctgtccttgc tccatcggtt ggccccgtcc tggcggctgt ctctcacggc ggtccatttc
     1261 aattatggcc tgcggggggg agaatcgcag ggtgacgaat cgttcgttcg aacctggtgc
     1321 ggcgaatggc gacttcccct tattgttcaa cggccggtgt tacccaagaa ccgcagagct
     1381 tcctcgctgc aggcagtcgc tcgcgaggtg cgctataccg cgatgcaaca gatggccagc
     1441 gatctggggg ccgaacggat cgtggttggg catacggcga acgatcaagc tgaaacgatg
     1501 gtgatgtgga tgctacgagg ggcgggcctg agaggattag ccggcatgcc gtacatgaga
     1561 gagggaatcg ttgttcgccc gctcttggct tcgacgcgag aagacatcct gacatatctg
     1621 gatcaacagg gggtgcccta tcggtctgat tcaagcaatg acaagccgct gtatcaccgc
     1681 aaccgaattc gaagggagat catgccggcc ttgacgcggc tgagaccggc tgctgtccgt
     1741 gtgctccagc gacatgcaga tttacttcga gacgatgatg cctacctgga gcagaccgtg
     1801 gaggggctga tgtcgtcatt tgtgagcaac gatgaggaag ggcgtcagcg attggatcgg
     1861 caagggattt cgattcttcc ggtggcgttg cagcggcgac tgatccgtca gattcttcgg
     1921 aggtgcgatg aacagaagcg tgcccccggc gtacggctgg tcgaagccgt ccgacaattt
     1981 cttctgaaag gacagccggg ggatcgactg atggtgcgat ccggtatgtt gattcagaag
     2041 cacgattgca cggaactctc ccgttctcga aatggatgtc tgacaggagc acctgacgca
     2101 gatcgagact cgaccgcggc atgctgtttg gccattccct cagtggcgtg ttggggtaga
     2161 aggaattttc aatttcaggt acaactcatg acgcgtgacg aggcagcgca attgcccccc
     2221 gcgcggtggg cgcatcgagc tttgtttgat gcagatcgat tctcgacgcc actggtggtc
     2281 agagcatggc aagcgggcga tcgcttttgt ccaacaggaa tgcgggggcg caccaagaaa
     2341 ctccaggacc tgtttactga tttgaagatt aggcgtgaag aacggggcga cattccgctt
     2401 ctggtggcat ctgaggggat tctctgggtt gtgggcaaac gacaggatga ccggtttgcc
     2461 gtccgcaact cgacaaccag gtgtttggtc gtcacggtga tggcgaggag cgaacgggaa
     2521 ggagcgctgt aggatggaac gactcttcgg tcgaccgatt gtgactcagg aggaaatgcg
     2581 cacccgcatt cgagaactcg gcaaacaaat tacggccgat tattcgggca aagactttgt
     2641 cctcgtcggg gtgctgaaag gagcctatgc attctatgcc gacctggctc gggcaatcag
     2701 aattcccatg cgggtggatt tcatcgtcgt caccagttat gggtcacagg caaagacatc
     2761 cggcaaagtg aaaatggtaa ccgagttgac cgaggacatc aaagggaagg atgtcttgct
     2821 ggtggaagat atcgtcgatt cgggtctgac gatccaatac ttagtcaagc aattgtcgaa
     2881 acggaaacca cggtcggtga agatctgcac gttgctcagc aagccggaac ggcggaccgt
     2941 cgacgttccc ctggcctata ttggtttcaa catcccggac aaatatgtcg tcggctacgg
     3001 attagactac cagcagaaat accggaacct gccctatttg gctgtgctgg atgtggatgg
     3061 aaagcaggag tagagtttgg gcatacggtt gtcagtcgga atacccttgt gttaacatca
     3121 cccaagtttg cggttttcct cgctggttag gcttgttgcc tataaggaga actggatgaa
     3181 ttccagagca aagaaccttc tcttctgggt cgtggtcggc ctcttcatga ttctgctgtt
     3241 caatctgttc agcgttccga cgcacgcgcc ggaagaagaa gtcattttca gcgactttat
     3301 ggccaagctc gacaaagggg atatcgaaaa agtcattatc aagtccagtc atatcagtgc
     3361 aattctaaaa gataagacgc ggatccgaac ctactctgcc gaatatcctg aacttgtcaa
     3421 agtgcttcgg gaaaagggcg tacagatcga agcgaaaccg ccggacgaaa gcccctggta
     3481 cattacgttt ctggtcacat ggggaccatt tgttctgttc ttagggcttt ggttcttctt
     3541 gatgcgccag atgcaaatcg gcggcaacaa ggctttatcg ttcgggaaaa gccgcgcccg
     3601 tatgttaaca gaggaacgga agaaggtgac cttctccgat gtggccggta tcgatgaagc
     3661 aaaagaggaa gtgctggaga tcatcgagtt tctcaaggat ccgagaaaat tccagaagct
     3721 cggcggccgt attcccaaag gggtgttgat cgtcggtcca cccgggaccg ggaagacatt
     3781 attggccaag gctattgcag gagaagccgg ggttccattc ttcagcatca gcggctccga
     3841 cttcgtggag atgtttgtcg gcgtcggcgc gtcccgtgtt cgtgatctgt ttgagcaggg
     3901 caagaagcac gcgccttgca tcattttcat cgatgagatc gatgccgtcg ggcgactccg
     3961 tggagcgggg ctcggtggcg gccatgac
//
LOCUS       DATDNW010000281         3210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1334625, whole genome shotgun sequence.
ACCESSION   DATDNW010000281 DATDNW010000000
VERSION     DATDNW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334625"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VI359_06745"
     CDS             <1..246
                     /locus_tag="VI359_06745"
                     /inference="COORDINATES: protein motif:HMM:NF045168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEY6973984.1"
                     /translation="FDPAAFIVRRSMPWADAYQAGLVFVAFGKSFDAFDGLLRRMIGA
                     EGGIMDALFTFTRPVSGSYFWCPPMKNGHLDLHAIGL"
     gene            246..668
                     /locus_tag="VI359_06750"
     CDS             246..668
                     /locus_tag="VI359_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973985.1"
                     /translation="MRFDPALAAQAAFHKAAAELGSDWDTAVELEETFSSNAGSAARE
                     AHEKLLALAQRYPNAHSFQAFCVYITWQQVTEETIARHFQTGMKLSEAYLASSVGKDP
                     HDLACVTELYASFRAGLGLEEEHEIQVEFRRDTPKGGD"
     gene            741..1514
                     /locus_tag="VI359_06755"
     CDS             741..1514
                     /locus_tag="VI359_06755"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6973986.1"
                     /translation="MADEDKHRARIFLHRGQLAQAKAAYEQAVADDRLTSDNAALSDS
                     LGNLGNVCALSGDLTQAESCYREVLNIQRTERNQHAIAHTLVNLGNLHFGTDTPEKAR
                     PYYLEALDLLKPLNDHRALGILYHNLGMEEARQSNWDQAVAYFTDALESHRVVGNEEG
                     LALTYSQLGKTFLDAGLLRKAEKCFNNASEHFIKLGNPSGEAAVLRLLADMYIRVQDS
                     VSAIRCLERVVSLGTHYRFVDVGQDQDRLHQIRQSTSRP"
     gene            1705..2265
                     /locus_tag="VI359_06760"
     CDS             1705..2265
                     /locus_tag="VI359_06760"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY6973987.1"
                     /translation="MAIIATSAHAADPAPQVTIGTQEVGLSAGYLLPERLTKHHVTKQ
                     QGPAFMPSWMITLTDPIGDGWYRGQVSIGAEVVYIQFQEPVLTHGVGFNPKIKYTFVA
                     DNRIRPYVEFAGGPFWTDLGNKIPEESSELNFILTAGVGISFFVTSQASINAGYRFQH
                     FSNAGTSYPNYGINSSLPFGGFSYFF"
     gene            2278..3021
                     /locus_tag="VI359_06765"
     CDS             2278..3021
                     /locus_tag="VI359_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973988.1"
                     /translation="MMTRHSRILTHGLAGLVVLVASGCSHWIELTDQATPRSSSQATI
                     ASDLRVPLLLETVHITQNGSPLATPPALERQVLGALETTQLFSQHFQSGYAQPAPNQA
                     HVTIRLSMDNHVEPHAGETAFRGFVIGASMFLLAPVISLDYDYGTEMALEVERWDGQT
                     KQYTASSSGSAHYYLFGASPHVIEELKGQVTDACLTSLLDQLVRDHTFYLADSSSQSE
                     SGIRTVSVGTRQPIADVHPVSTNTPASSP"
BASE COUNT          679 a          940 c          840 g          751 t
ORIGIN      
        1 ttcgaccccg ccgcgttcat tgtgagacgg tcgatgccgt gggccgatgc ctaccaggcc
       61 ggattagtat ttgtggcgtt cgggaaatcg ttcgatgcgt tcgacggact tctacgcagg
      121 atgatcggag cagaaggcgg catcatggat gccttgttca ccttcacacg tcctgtttcg
      181 gggagctatt tttggtgtcc cccgatgaag aatggacatc ttgacttgca tgccattggt
      241 ctctaatgcg ttttgatccg gcgctcgccg ctcaagcggc gtttcacaaa gcagcagccg
      301 aactcggctc agactgggat acagccgttg agctggaaga gacgttttca tccaacgccg
      361 gatcggcagc acgggaggcg catgagaaac tgcttgctct cgcccagcgc tatcccaacg
      421 cccattcctt tcaagcgttc tgcgtctaca tcacgtggca acaggtgacc gaagaaacga
      481 tcgcacgtca ctttcaaacc gggatgaagc tatccgaagc gtatcttgct tcctcggtcg
      541 gcaaagaccc gcacgatctc gcctgcgtca ctgaactcta tgcatcgttt cgcgccggat
      601 tggggcttga ggaagagcac gagattcagg tggagtttcg gcgggatacg ccaaaaggag
      661 gagactagct cctcgaagag cgacgctgag cgtgatgagc gtgggaatgt cgggagctga
      721 atgctgacgg cttatggctc atggctgatg aggacaaaca tcgcgcgcgc atctttctcc
      781 atcgcggtca actcgcgcag gccaaggcgg cctatgaaca ggccgttgcg gatgatcgcc
      841 tgaccagcga caacgccgcc ctatcggatt cactgggtaa tctcggaaat gtttgcgcgt
      901 tgtcgggcga tctgacacag gcggaatcct gctaccggga agtcctgaat atccagcgca
      961 cggaacgcaa tcaacacgcg atcgcccata cgttggtgaa tctgggcaac ttgcacttcg
     1021 gaaccgacac accggaaaag gcccgcccct actatctgga agcgctcgat ctgctgaaac
     1081 ccctgaacga tcatcgcgcc ctcggcattc tatatcacaa tcttgggatg gaagaagcgc
     1141 gccaatcgaa ctgggatcag gcggtcgcat atttcacgga tgcgctcgaa tcgcatcgcg
     1201 tggtcggcaa tgaagaaggc ttggccctca cctatagcca actcggcaag acgtttctgg
     1261 atgcgggcct cctgcgcaaa gcagagaagt gtttcaataa cgcctccgag cactttatca
     1321 aactggggaa tccttcgggc gaagccgccg tccttcgact ccttgccgat atgtatatcc
     1381 gcgtgcagga ctcggtatct gccatccgtt gtcttgaacg agtcgtgtct ttgggcactc
     1441 attaccgttt cgtcgatgta ggccaggatc aggaccggtt gcaccagatt cgtcagtcaa
     1501 cttcccgccc ctaattgccc cttgagagtt ttcacctatc atcggacaga atttcacttg
     1561 gttctggaca gataacgcgg ctctggtacc ttgggaacgt cacgcctgtt gattttgttt
     1621 cgtaagaacc agtcagcagg attctgacgc gaatctctca ggcaggttga gatcttgttc
     1681 gttcctcttt gtgtgatggg gctcatggcg atcattgcca catccgctca tgctgccgat
     1741 ccagctcctc aggtcaccat cggcactcaa gaggtcggtc tctcggctgg atatcttctt
     1801 cctgagcgat taaccaaaca tcacgtgaca aaacaacaag gtcccgcttt catgccttcg
     1861 tggatgatca cgctcaccga tcctatcggc gatggctggt atcgcgggca agtctcgatc
     1921 ggcgccgagg tggtgtatat ccaattccag gaaccggtcc tcacgcacgg cgtcggattt
     1981 aatccgaaga tcaaatacac tttcgtggca gataaccgga tccgcccata tgtcgaattt
     2041 gcaggaggcc ccttctggac cgatcttggt aacaagatcc cggaggaatc ctctgagctg
     2101 aacttcatct taactgcggg cgtcggaatt tcgtttttcg tcacgtcgca ggcttcgatc
     2161 aatgccggtt atcgctttca acacttttcc aatgccggta cgagttatcc aaactatggg
     2221 atcaattcca gtcttccatt cggcgggttt tcctactttt tctagttgag gcaccgcgtg
     2281 atgactcgac actcccgaat tttaacgcat ggcctcgctg gcctcgtcgt cttggtggca
     2341 agcggttgct ctcactggat cgagctgact gaccaggcaa ctccgcgatc ctcgtcacag
     2401 gctacaatcg ccagcgacct ccgcgtcccc ctgctccttg agacagtcca tatcacacag
     2461 aacggctccc ctctggctac tccgccagct cttgaacgcc aggtgcttgg ggccctcgaa
     2521 accacgcagc tcttttccca gcactttcag tctgggtatg cacaaccagc accaaaccag
     2581 gcccatgtga cgattcgact ctcaatggac aatcacgttg aaccccatgc cggcgagacc
     2641 gccttccgtg gattcgtgat cggggcttcc atgtttctcc tggcaccggt gatctcactc
     2701 gactatgact acggcacgga gatggccctt gaggtggagc gatgggacgg ccagaccaag
     2761 caatacacag cttcctcgtc cggcagtgcg cactactatc tattcggtgc aagcccgcac
     2821 gttatcgagg aactgaaagg gcaggtgacg gatgcctgcc tcacctcgtt gttggatcaa
     2881 ctcgttcgcg accacacctt ctatctcgcc gatagttcgt ctcaatctga atccggtatc
     2941 cgcacagtct ccgtgggcac tcggcaaccg atcgccgatg tacatcccgt gtcaacgaac
     3001 acacctgcct cctcaccgta gcagtctttc ctcgccctga agatcgagca cctacagttg
     3061 accatcgctc ccgatagggc tagtcgtcca gttccctccg atggtatcat tgcgaagatt
     3121 attcttctgg acgtcacaat ggacctggac gcattccgac agatggtggc caaaaatccg
     3181 aagggctttc tgggccgcta tggattgggc
//
LOCUS       DATDNW010000282         4990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1199223, whole genome shotgun sequence.
ACCESSION   DATDNW010000282 DATDNW010000000
VERSION     DATDNW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199223"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VI359_06770"
     CDS             complement(<1..793)
                     /locus_tag="VI359_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY6973989.1"
                     /translation="MKNSAQQLRQAFVRYFEQQGHQAVPSSALIPQADPTLLFTNAGM
                     NQFKRVFLGEETRPYKRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKEDAIRFGWEFLTQTAGLAKDRMWVTIFREDDEADRLWKQIGVPASRIVRCDEKDNF
                     WQMADTGPCGPCSEIHFDQGPSVPGDDRPNGQGDRVIEIWNLVFMQFNRDTSGKLHPL
                     PKPSIDTGMGRERLAAVAQGVFSNYDSDLFMPLLTVIANRAGMQYG"
     gene            complement(790..1290)
                     /locus_tag="VI359_06775"
     CDS             complement(790..1290)
                     /locus_tag="VI359_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY6973990.1"
                     /translation="MKSGGTGRGIGPSQPNQWLHLAVRYLARWDRTAGQVEHFLKDKG
                     ATPAQVRQTINRLSELRYLNDRAYAQRFIESRLARKPMGRERLKGELLARGVGESLAD
                     RAIREVLRGVDEEALARRALKVQQGQGGRLAPMRAARLLRQRGFEEETIDRIIGVSGE
                     TMEIDA"
     gene            complement(1268..2341)
                     /gene="recA"
                     /locus_tag="VI359_06780"
     CDS             complement(1268..2341)
                     /gene="recA"
                     /locus_tag="VI359_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY6973991.1"
                     /translation="MAEKDDKKRALELALSQIEKQYGKGAIMKLGAEERQADVPAIST
                     GSLGLDIALGVGGLPRGRVIEIFGPEASGKTTMTLHCIAEVQKTGGVAAFIDAEHALD
                     LTYAKKLGVQADDLLVSQPDTGEQALEIAETLVRSGAIDLIVIDSVAALTPRAEIEGE
                     MGDAHMGLQARLMSQALRKLTAAIAKSLTTVIFINQIRMKLGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKEGQDVMGSRVRVKVVKNKMAPPFKQAEFDIMFAEGISKTGEL
                     IDVGVEKRLIEKSGAWYSYKGERIGQGREAARDYLKHNPGAAREIEAKLRELAGVPAR
                     GERKPDAKDEKPAAKGDEKRGHR"
     gene            complement(2528..2920)
                     /locus_tag="VI359_06785"
     CDS             complement(2528..2920)
                     /locus_tag="VI359_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973992.1"
                     /translation="MKLVFPIATLFIVLAIPAWAGGSEEFDPDEPFRDAVTKHLLRSW
                     FNHALDVLDDHLEITGTLDPDNAKGDRGSHLRLKFYPEGKSTSDESITAEGWVDRSPD
                     GRRQELHFRFTLPESSSENTLEQFERVL"
     gene            complement(2952..3563)
                     /gene="thpR"
                     /locus_tag="VI359_06790"
     CDS             complement(2952..3563)
                     /gene="thpR"
                     /locus_tag="VI359_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY6973993.1"
                     /translation="MIRAFLAVEVKDDLRKQIAQVQQDLKQRLSREAPREVRISWVHP
                     TSIHLTIKFLGDIDEQLVDPMRAAIGQVLREHRAIQIPLERLGAFPRPQQPRVLWIGA
                     SEQWEQREDGKRLEGFHRAIEDCCATFDFAPEGRPLSPHLTLARIKEGERHIGQALAK
                     SGVMDRSLTLTSLAIESVVLMKSELRPTGSVYTKLWEIALAHP"
     gene            complement(3560..4855)
                     /locus_tag="VI359_06795"
     CDS             complement(3560..4855)
                     /locus_tag="VI359_06795"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY6973994.1"
                     /translation="MPTSAALLLTAETIAIGTELLIGGKSDANSLFLADELGKLGIEV
                     RFKSLVGDTQSDIALVIGHAAKRAGVVIMTGGLGPTMDDRTREAVAQATGRRLGKRKE
                     ALDGMKVRLAQWGRTPNKGQLRQTLIPSGATVLANPVGSAPGFCLTWKNSLIVSLPGV
                     PREMEAMMVQEVIPILKSRLSDTRQMAGPIVRQVFHTFGLPEADVDDKLKGLIPKGVP
                     VDLGLLASPMGVLVSLTTKSGPRKNRITGDLLQSLASQLRSRLDGWLFAEGHQTMEEV
                     VGRLLTKQSLTVAVAESCTGGLIGHRLTQVPGSSAYFDRGIVCYSNGAKTELLGVPTS
                     LLEKHGAVSAPVAAAMANGIRERSGTDIGLSVTGIAGPDGGTATKPVGLVFIGFDPGE
                     GETMTKEFRFHGDRHVIKQRSSQAALDVLRRWLIDRESE"
BASE COUNT         1011 a         1557 c         1323 g         1099 t
ORIGIN      
        1 tgccgtactg catgccggcc cgattcgcaa taaccgtcag cagcggcatg aagagatcgc
       61 tgtcatagtt gctgaagacc ccctgcgcca cagctgccaa ccgttcccgt cccataccgg
      121 tatcgatgct cggcttcggc agcggatgca acttccccga cgtatcccga ttgaattgca
      181 tgaagacgag gttccagatt tcaatgacgc gatcgccctg gccgttcggc cgatcgtcgc
      241 ctggaacgga cggaccttga tcaaaatgaa tctccgaaca gggaccgcag ggacctgtat
      301 ccgccatctg ccagaagttg tccttctcgt cgcaacgcac gatgcgtgag gccggcaccc
      361 cgatttgttt ccacaaccgg tccgcttcat catcctcccg gaagatcgtc acccacatcc
      421 ggtccttggc caagcccgct gtctgcgtca gaaattccca accgaatcta atcgcgtctt
      481 ctttgaaata gtcaccgaag gaaaagttcc caagcatttc gaaaaacgta tggtggcgtc
      541 gcgtgtagcc gacgttttca agatcgttat gcttcccgcc tgcccgcaaa catttctgaa
      601 ccgtcacggc ccgcttatag ggacgagttt cttcgccaag aaacacccgc ttgaactgat
      661 tcatcccggc attcgtgaac agcagagtgg ggtcggcctg gggaatcaac gccgagctcg
      721 gcacggcctg atgcccttgc tgttcgaaat accggacgaa cgcttgtcga agttgttgcg
      781 cactattctt catgcatcaa tttccatggt ctcgccggac acccctataa tacggtcaat
      841 cgtctcttcc tcgaaacccc gctgacgtag gagacgagcc gctcgcatgg gagcgagccg
      901 tccaccttgt ccttgctgga ccttgagggc ccgacgcgcc aaggcctctt catcaactcc
      961 tcgcaggacc tcacggatgg cccgatcggc aagagattca ccaacacctc ttgccagtaa
     1021 ctcccccttg agccgctccc gtcccatcgg cttcctggcc aagcggctct ctatgaatcg
     1081 ttgagcatag gcgcgatcat tgagataccg aagctcggat aaccggttga tggtctgtct
     1141 gacctgagca ggcgtggcgc ccttgtcctt gagaaaatgc tcgacctgac ctgccgtgcg
     1201 gtcccagcgg gcgagatacc gcaccgcaag gtgcagccac tggttcggct gtgacgggcc
     1261 gatccctcta ccggtgcccc cgcttttcat cacccttggc ggccggcttc tcgtctttcg
     1321 cgtcgggttt cctctcgcca cgtgccggta cgccggccag ttcgcggagc ttcgcttcga
     1381 tctcccgggc cgcccccgga ttgtgcttga gatagtcgcg cgccgcctca cggccctgac
     1441 cgatccgctc acctttatag gagtaccagg ctccggactt ctcgatcagg cgcttttcca
     1501 ctcccacgtc gatcagctcg cctgtcttcg aaatgccctc tgcaaacatg atgtcgaatt
     1561 ccgcctgctt gaagggcggt gccatcttgt tcttgaccac tttcacgcgg acgcggctgc
     1621 ccatcacatc ctggccttct ttgatcgatt cgatgcggcg aatgtcgaga cggaccgagg
     1681 agtaaaattt gagcgcgttg ccgccggtcg tcgtttcagg attgccgaac atcacaccca
     1741 gtttcattct gatctgattg ataaagatca ccgtcgtaag tgacttcgca atcgccgccg
     1801 tgagtttccg caacgcctgc gacatgagcc gcgcttgaag gcccatgtgg gcatcgccca
     1861 tctcgccttc gatttcagcc cgaggcgtca gcgcagcgac ggagtcgatg acgatcaagt
     1921 cgatcgcgcc actgcgcacg agtgtttcgg caatttccag ggcctgttcc cctgtatccg
     1981 gttgggacac cagcagatcg tcggcttgca ctcccaactt cttcgcatag gtcagatcca
     2041 gcgcatgctc cgcatcgata aacgctgcca ccccgccggt tttctgcact tcggcgatgc
     2101 aatggagtgt catcgtcgtc ttgcccgaag cttccggacc gaaaatctcg atcacgcgcc
     2161 cgcgcggcaa tcccccaaca ccgagggcga tatcgaggcc caacgacccg gtcgaaatcg
     2221 ccggaacgtc ggcttgtctc tcctcggccc ccagcttcat gatggcaccc ttcccatact
     2281 gcttctcgat ctgggagagg gctaattcca gcgcgcgctt tttgtcgtct ttctctgcca
     2341 tgaaggtctc ctacgttatt gaaggtcagg cgcggggatt ataccgaaga gagaaaggga
     2401 aacctagccc agaccctcat caggtctcgt ccatgatact tcgatgtacg cgaaatacag
     2461 ggatttcaca cgcaacagcc cacactatct gacatcaccg aatggaacac cgccggcaaa
     2521 ggagaggcta gaggacgcgc tcaaactgtt ctaaggtgtt ctctgaagaa gactcaggaa
     2581 gagtaaatct gaaatgcaat tcctgacggc ggccatctgg ggatcgatca acccagccct
     2641 cagcagtgat cgactcatcg gacgttgatt tcccttccgg gtagaacttc aatcgcagat
     2701 ggcttccgcg atcacccttt gcgttgtccg gatccagagt gcccgtgatc tccaggtgat
     2761 cgtcgaggac atctaacgca tgattgaacc atgatcgcaa cagatgcttg gtcaccgcgt
     2821 ccctaaaggg ctcatcaggg tcgaattctt cggacccgcc tgcccatgca ggtatcgcca
     2881 acactatgaa aagagtcgcg atcggaaaaa ccagtttcat gggcatatct tataaacgcc
     2941 gcggcggacc gtcaaggatg ggcgagggca atctcccaca gcttggtgta caccgacccg
     3001 gttggtcgca gctcactctt catcaacaca acggattcga tcgcaagcga tgtcaacgta
     3061 agcgaccgat ccattacacc actcttggcc aaagcctgtc caatgtgacg ttccccttct
     3121 ttgattcgcg ccagcgtcaa atgcgggctc aagggcctgc cctcgggagc aaaatcgaag
     3181 gttgcgcaac agtcttcaat ggctcgatgg aacccctcca gtcgtttgcc atcttcacgt
     3241 tgctcccact gttctgaggc accgatccag agcaccctcg gttgttgcgg acgtggaaag
     3301 gctccgagcc gttcgagggg aatctggatc gctcggtgtt ccctcaggac ttgcccgatc
     3361 gccgctcgca tgggatcgac tagctgttcg tcgatgtctc cgagaaactt gatcgtcaga
     3421 tgaatcgagg ttgggtggac ccaggaaatg cgcacctctc tcggggcttc tctgctcagt
     3481 cgctgcttga ggtcttgctg cacctgcgca atttgcttgc ggaggtcgtc tttcacttcg
     3541 accgcgagga aagctcgaat cattcggatt ctctgtcaat cagccagcgt cgaagcacat
     3601 cgagggctgc ttgcgaggat cgttgtttga tcacatggcg atcaccgtgg aagcgaaact
     3661 cctttgtcat cgtctctccc tctcccggat cgaagccgat aaaaaccaac cccaccggct
     3721 tggtcgccgt tccgccatca ggaccggcga tgccagtcac actgagaccg atgtcggtcc
     3781 cgctccgctc ccgtatgccg ttggccatcg cagctgcgac gggcgcgctc accgctccat
     3841 gcttctcaag cagactcgtc gggacaccca ggagttctgt cttcgccccg ttgctgtaac
     3901 agacgatccc tcgatcgaag tacgccgacg agcccggcac ctgcgtcaat cgatggccga
     3961 tcaagccgcc ggtacaagat tcggccaccg caacagtgag actctgcttc gtcagcaacc
     4021 gtccgacgac ttcctccatg gtctggtgcc cttcggcaaa gagccaacca tctaatcgag
     4081 agcggagttg cgacgcgagc gattgaagaa ggtctcccgt gatcctgttc ttccggggtc
     4141 cagatttggt cgtgagcgag acgaggacgc ccatcggcga ggcgagcagg cccagatcga
     4201 ccggcacccc tttgggaatg agtcctttca gcttgtcatc aacatcggcc tccggaaggc
     4261 cgaacgtgtg aaagacctgg cgcacgatcg gtcctgccat ctgtctggtg tcgctgagtc
     4321 tcgacttgag gattggtatc acttcctgaa ccatcatcgc ttccatctct ctcggcacgc
     4381 ccggcaaaga cacgatcaga ctgttcttcc aggtgaggca aaatcccgga gccgatccga
     4441 ccggattcgc gagtacggtc gctccagaag gaatcaatgt ttgccgcagt tgtcccttgt
     4501 tcggcgtgcg accccactgg gccagacgga ccttcatgcc gtccaacgcc tccttccgtt
     4561 tgccgagtcg ccgaccggtc gcctgagcga cggcttccct tgtgcgatcg tccatcgtgg
     4621 gaccgagtcc gccggtcatg atgacaacgc cggcgcgctt cgccgcgtgt ccgatgacaa
     4681 gagcaatgtc cgactgcgta tcgcccacaa gcgacttgaa ccgcacttcg atgcccagtt
     4741 tgccaagttc atcggccaaa aacagcgagt tggcatcgga ttttccaccg atcaagagct
     4801 cagttccaat ggcgatggtt tctgcggtga gtagcagggc tgcggatgtg ggcatggatg
     4861 gcagagcgtc gtggaaatta gtgaatctct gaaaagttca gtgtaaaggt tgtttcacct
     4921 ccgttggccg ggaacactgt gatgttcgtc ttagccaacg gatcgaagtc ggcatagttg
     4981 aacgaggcca
//
LOCUS       DATDNW010000283         4230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_925691, whole genome shotgun sequence.
ACCESSION   DATDNW010000283 DATDNW010000000
VERSION     DATDNW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925691"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VI359_06800"
     CDS             <1..1423
                     /locus_tag="VI359_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEY6973995.1"
                     /translation="SAWKKAGESEWALRSNPSSGNLHPTEGYVLLPQIDGLDLKPGLY
                     HYAPKEHALELRAEFSADHVARLLDPFPQNAFLFGLTSVHWREAWKYGERAFRYCNHD
                     AGHAIGTARIAAATLGWNMTLLDGVAQNTIAMLLGTHRTDDFREVEPEHPDCIAVIWP
                     HWNVKREEPTEVPLYLDPAAAKEASQAPWYGKANRLSHDHGVHWEIIDEAAAATWKLS
                     EERHSVVISRADTMATSRTTSDTQPKAGQIIRQRRSAVSFDGKTSISAATFFRMMQLV
                     MPWIERPQLERPMPWDVWPYDPAIHLLLFVHRIDGLTPGLYFLVRNPEKLTFIQGSMN
                     PELNWTLARGAPDGLPLYWLLEGDARRLAGQVSCHQDIAGDSAFSFGMLAEFEGRLRE
                     GGAWWYPRLFWESGLLGQVLYLEAEAAGVRATGIGCFFDDPVHEIVAVKGLSLQSLYH
                     FTIGGPVEDRRLMTLPAYHHLDK"
     gene            1469..2323
                     /locus_tag="VI359_06805"
     CDS             1469..2323
                     /locus_tag="VI359_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6973996.1"
                     /translation="MFKIKKKIDKPKAPVLYNVIEDYSEFDAPGNVTVCAMTENEDLS
                     GHAKILSESYDVPLDTMTTLLTEGGAYVYPQISGLLTRGLFACLVDSTGGGPKQTFVL
                     DLMQFAEAEQLARARSLPLVEAALEIFRRTLPQELKTKLQQKNLGLDYRTLDRAVAKG
                     GDIKYIDFRKDWSPHFKRLCIMPDGRLIETGGLEEFAQLHGITTSQAKTLVEQGGTLE
                     VNGEVLACQIVNGQPAVARFSAKNYTKAKELVATKGLHLMDALSEVGYNDPAMMRGLV
                     RKELASGR"
     gene            2375..2788
                     /locus_tag="VI359_06810"
     CDS             2375..2788
                     /locus_tag="VI359_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEY6973997.1"
                     /translation="MKRSGVLWLVIALIAACSPKPILYPNAHYQQVGEAAAEQDVEEC
                     KEMAKDAGAKSSQGKTGQVAGGTAAGGAVGSAAGAVGGAVVGHPGRGAMVGAAGGATA
                     GFLRSLFRKSPPSNAYKQFVQRCLTERGYEPVGWE"
     gene            complement(2751..3230)
                     /locus_tag="VI359_06815"
     CDS             complement(2751..3230)
                     /locus_tag="VI359_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY6973998.1"
                     /translation="MSDETNPASARIIQALAIAWGLGIVSIGVLPLDNFVGHSHWEYI
                     KWFPTFDNLRSWRYIFDMTANTVLFIPYGYFLTRSIGNSPALSTRVAVGSAALLSSSI
                     EFYQVYCHNRHPSLLDIITNTLGSFIGATIAVLIGRMRRTPPADRITPIPPARTPAQ"
     gene            complement(3375..3737)
                     /locus_tag="VI359_06820"
     CDS             complement(3375..3737)
                     /locus_tag="VI359_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6973999.1"
                     /translation="MATILVIDDEDSIRTLLKDVLEKAGHRVIQAADGRAGLTAYQKN
                     KVDLVLMDILMPDTDGLEATLQLTREYLDAKIIAMTGAQGDKNFLDVAKLFGARRVFE
                     KPFDLAKLVQAVNEELAR"
     gene            complement(3827..>4230)
                     /locus_tag="VI359_06825"
     CDS             complement(3827..>4230)
                     /locus_tag="VI359_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974000.1"
                     /translation="ALDLKGQLPSSGQPARSDQKALSMVSGRDNAISYISLASALKAQ
                     EDGIPIQILTIDKVEPGEPTVKNGRYRLRRPVLLLTGTQPDPLTESFVTFMQSPEGQL
                     LIRTLYVPLDHPSPAAPTTNARQPEPTAPTL"
BASE COUNT          921 a         1048 c         1301 g          960 t
ORIGIN      
        1 gtcagcgtgg aagaaagcag gggagtcgga atgggcgctg cggagcaatc cgtcgtccgg
       61 gaatctccac cccacagagg gctatgtgct cctgccgcaa atcgacggac tcgatctcaa
      121 gcccgggctg tatcactacg caccgaagga gcacgcgttg gaattacgtg cggaattttc
      181 agctgaccat gtggcgaggc tgctcgatcc gtttccccag aatgcatttc tctttggcct
      241 gacctctgtt cattggcggg aggcctggaa gtatggagag cgggcgtttc gctattgcaa
      301 ccacgatgca ggccatgcaa tcggcacggc gcggatcgcg gctgcgactc tcggttggaa
      361 catgacgttg ctggatggtg tggcgcaaaa taccattgcg atgctgctgg gcactcatcg
      421 caccgacgat tttcgcgagg tggaaccgga acatccggat tgtattgcag tcatttggcc
      481 acattggaac gtgaagcgcg aggaacccac ggaggttccc ctgtatcttg atccggcagc
      541 agccaaggag gcgtcacagg caccatggta tggaaaagcc aatcggttga gccatgatca
      601 cggtgtgcat tgggagatta ttgatgaggc ggccgccgcc acgtggaaac tttcggagga
      661 gcgacattcc gttgtcattt cacgcgccga tacgatggct acttcacgaa caacgagcga
      721 cactcaacca aaggccgggc agatcatccg ccagcggcga agtgccgtgt cgtttgatgg
      781 gaagacgtcg atttccgccg ccacgttttt ccggatgatg cagttggtga tgccgtggat
      841 cgaacgtccg caattggagc gccccatgcc gtgggatgtc tggccgtatg atccggcgat
      901 tcatttacta ttgtttgtcc atcgcatcga tggtctgacg ccggggctct attttcttgt
      961 ccgcaatccg gaaaaactga cctttattca gggatccatg aatcccgagt taaattggac
     1021 gctcgcacga ggggcgccgg acggtctgcc gctctattgg ttgctcgaag gcgacgcgag
     1081 gcgattggcc gggcaagtga gttgccatca ggacattgcc ggggacagtg cgttctcgtt
     1141 tggcatgttg gctgagttcg aaggacgctt gcgggagggg ggagcctggt ggtatccacg
     1201 tctcttctgg gagtctggtt tgcttgggca ggtgctctat ctggaagcag aggctgctgg
     1261 tgtacgggcg accgggatcg gatgtttctt tgatgaccca gtacatgaga ttgtggcggt
     1321 gaaaggtctg agcctgcaat cgctctatca ctttacgatc ggtggacctg tggaggatcg
     1381 gagactcatg acgttgcccg cctatcatca tctcgataaa taagtttatg cgactctaat
     1441 aattcaatat gttcgatagg aatagctcat gtttaaaata aagaagaaaa tcgacaagcc
     1501 gaaggctcct gttctgtata acgtcatcga agactattcc gagtttgacg cgccgggaaa
     1561 tgtgacggtc tgcgccatga cggagaatga agatctgtcc gggcacgcga aaatcctctc
     1621 cgaaagttat gacgtgccgt tggacacgat gaccacactt ctgaccgagg gaggagccta
     1681 tgtctatccg caaatcagtg gcttgctgac gcgcgggtta tttgcctgct tggtcgattc
     1741 gaccggcggg ggtccgaagc agacctttgt gctagatctg atgcagtttg ccgaggcaga
     1801 acaacttgcg cgggcccggt ctcttccgtt agtcgaggct gcgttggaga tctttcggcg
     1861 aaccttgccg caagagttga agacgaaact gcaacagaag aatcttggct tggactatcg
     1921 gacgttggat cgcgccgtgg cgaaaggggg cgatattaag tacatcgact tccgcaagga
     1981 ctggtcgccc cacttcaaac gtctctgcat catgcctgat ggccgtctca ttgaaaccgg
     2041 agggctagaa gagtttgctc agttacacgg cattacgact tcacaagcca agacgctcgt
     2101 cgagcagggc ggaacactcg aagtcaacgg cgaggtgctg gcctgtcaaa tcgtaaacgg
     2161 ccagccggcc gtggcgcggt tcagtgcgaa gaattacacg aaagctaagg agttggtggc
     2221 gacgaaaggg cttcacctca tggatgcgtt gagtgaagtg ggctacaacg atcccgccat
     2281 gatgcgagga cttgtccgca aggagctggc cagcggccga tgacagtcaa cgccgagctt
     2341 cctcattcgt tcttccatca caaaggattc tagtatgaag aggtcaggcg ttctttggtt
     2401 ggtgattgcg ctgattgcag cgtgctctcc caagccgatt ctttacccga atgcccacta
     2461 ccagcaagtt ggggaagccg cagccgagca agatgtcgaa gagtgcaagg aaatggcaaa
     2521 ggatgcagga gcgaagtcga gtcagggcaa gaccgggcag gtggcaggcg gcacagcggc
     2581 tggtggtgcc gtgggctctg ctgccggtgc cgtcggcggc gcggtggttg ggcatcccgg
     2641 tcgcggggct atggttggcg ctgctggtgg agccacggcc ggctttctcc gcagcctgtt
     2701 tcgaaaatct cctccaagca atgcctataa acaattcgtg caacgctgcc ttactgagcg
     2761 ggggtacgag ccggtgggat gggagtgatt cgatcggctg gtggagttcg ccgcattcga
     2821 ccgatcagga ccgcgatggt tgcgccgata aagctgccaa gtgtattggt gatgatgtcg
     2881 agcagcgatg gatgacggtt gtggcaatac acttgataga actcgatgga gctcgacaac
     2941 aaggctgccg agccaacggc gacacgcgtg ctgagagcgg gtgagttgcc aatcgatcgt
     3001 gtgagaaagt agccataggg gatgaagagg acggtgttcg ccgtcatgtc aaaaatgtac
     3061 cgccacgagc gtagattgtc gaaagtggga aaccatttaa tatattccca gtgcgaatgc
     3121 ccgacgaagt tgtctaaagg gagaaccccg atcgacacaa ttcccaatcc ccaggcaatg
     3181 gccaatgcct gaatgatgcg cgctgaagcc ggattcgttt cgtcagacat ggatgaacgg
     3241 tgttctgaac cgggtatcct ggagtagtct tcccccgcgt ccacacattt gtcgattgaa
     3301 atatcgcagc gtggatggag agccacacaa ttgcttctac tgaacaggat caggtagggg
     3361 aagacagcaa tgaattagcg agcgagctct tcgttcacgg cttgtacgag tttcgccagg
     3421 tcgaacggtt tttcgaacac gcggcgtgca ccgaagagct tcgcaacatc gaggaagttt
     3481 ttatcacctt gggcgccggt cattgcaatg atcttggcgt cgaggtactc gcgggtcaat
     3541 tggagcgtgg cttcgagtcc gtctgtatcc ggcattaaaa tatccatcag cacgagatca
     3601 accttatttt tttggtaggc cgtcaaacct gcccggccgt cggctgcttg aatgacccga
     3661 tggcctgcct tttccaacac atccttgagc agggtgcgga ttgaatcttc atcgtcgatg
     3721 acgagtatcg tagccatatg ctcgaactca cgcgtttcta cttgtgaatc ttacccgccc
     3781 tctttccgcc tgtctaggga gaaatgaatg gggacgggaa aatggatcac aacgtcgggg
     3841 ctgttggctc tggctgtcgt gcatttgtgg tcggcgcagc cggactcgga tggtccaggg
     3901 ggacatagag cgtgcgaata agcaactgtc cctctggcga ctgcatgaag gtaacgaagg
     3961 attccgtgag tggatccggc tgagtgccag tcaggaggag caccggccgg cgtagccgat
     4021 atcgtccatt cttcactgta ggctcgcccg gttcgacctt atcgatggtc aagatctgga
     4081 ttggaatgcc atcttcttga gccttgaggg ctgaggctaa cgagatatag ctgatggcgt
     4141 tgtctcgccc ggagaccatg cttaatgctt tttggtccga gcgtgccggt tgaccggatg
     4201 atggtagctg tcctttgaga tcgagtgcgg
//
LOCUS       DATDNW010000284         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_995052, whole genome shotgun sequence.
ACCESSION   DATDNW010000284 DATDNW010000000
VERSION     DATDNW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995052"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1587)
                     /locus_tag="VI359_06830"
     CDS             complement(157..1587)
                     /locus_tag="VI359_06830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY6974001.1"
                     /translation="MSESLIQKRAEVQGLATAILLPSIDLLDESGRLPVRRVGPLTLV
                     VGIGLFQRTVLTLQRAGIRQLIVLVGSEEDQLKQALGKGPRLTIPVRWMPIREFPLDD
                     SRTWESLAAESGGFCLVSGVRGVFSRQLIEHLRREVQEGQVMVVARASTVRDQQERRL
                     GLRITAQGERLLSLESSRQDDAALVATDVIVLPSGFISAVSREGIESGTVPLRRWLER
                     AAEEGRVRVLSTESNPSHWYQDLRDHADVQLAERKLFSSLKGEYEGFVDRFFNRKLSH
                     WFTRVFLAFGFSPNAVTLVATGIGLISAACFAFGSYQAGVIAAVLFQLSAVIDCCDGE
                     VARLTFTESPFGAWLDIALDNVVHMAIFVGIAAGSYLRVMGHEGAWVPLVLGAAAVLG
                     NALSFTLVSKAQKIKTASEWKTPVHAAWSDFMLKNVASRDFSVIVLLFACLDKLEWFL
                     WMAAIGSMSFVAVMLWVLRPSAMTRA"
     gene            complement(1597..2316)
                     /locus_tag="VI359_06835"
     CDS             complement(1597..2316)
                     /locus_tag="VI359_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEY6974002.1"
                     /translation="MKAIILAAGVGKRLWPVTQHRPKCLIQIGGQTLLHRYLTALASL
                     GIQRADIVVGYKQEMIRSAVEADSRGVRVNFLVNEQFHRGSISSLWIARTVLDDDVII
                     MDADVLFDREILRRLVQSPWPNCLLMDDTVKQTGEECMVVAEGGRVIALTKKMPLRYD
                     VAGEGVGFLKVRHADTPHVVASLRTYVDREAWHMEYEDALLEFFKNVKVGYETIGGLA
                     WTEIDFTEDVAKAEREILPKI"
     gene            complement(2313..3395)
                     /locus_tag="VI359_06840"
     CDS             complement(2313..3395)
                     /locus_tag="VI359_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6974003.1"
                     /translation="MILLNPGPVNVSERVRQALLRPDICHRETEFTDLLHRIQAKLLQ
                     AFVPGAESDYAAVVITGSGTSAVESALMSSIPHGKRMLVLNNGVYGERISQIVGLNRL
                     GVSELKYDWTARPDPERLRLALRQHPEVHAVAMVHHETTTGLINPVKEIAEVVDSQNR
                     VFILDAVSAMAGETLDIVGPHIYMAAGTAGKCIQGFPGVSFVLVRKGFLERMRKYPKR
                     TWYLHMTHYVDDQGLGTVPFTPAVQLYYAFDEALSELLEEGVAKRIQRYRNISTFIRD
                     RMAKLGIKPLLPPDYQSNTITAYHLPPGLSYARLHDALKEQGYVIYAGQGNLEGQIFR
                     IANMGVLSQAQIQGFLDAFERICTAA"
BASE COUNT          767 a         1121 c          997 g          699 t
ORIGIN      
        1 attatcatca cccgcccgcc cctaggcgcg ccaagacgcg ccgattgacc cagcgaggtc
       61 gcaaagagcg aaggccccga gccgtacggt tgtcggtacg tcgaggggaa taagcgactg
      121 agaacaaggc atgatgttgg atttagcatc ccactactaa gcacgagtca tagctgacgg
      181 acggaggacc cacagcatca cagcaacaaa cgacatcgat ccgatggcgg ccatccagag
      241 aaaccactcc agcttgtcca agcacgcgaa gagcagcaca atcaccgaga agtctcggct
      301 ggcgacgttc ttgagcatga agtccgacca ggcggcatgc accggcgttt tccattcact
      361 cgcggttttg atcttttgcg ctttgctcac cagtgtgaac gataaggcgt ttccaagcac
      421 cgccgccgca cccaagacga gcggcaccca tgcgccctca tggcccataa cccgcaagta
      481 cgagccggcg gcgatcccga cgaagatggc catatgcacg acgttgtcca gcgcgatgtc
      541 gagccaggcg ccgaagggag attccgtaaa cgtgaggcgc gcgacttcgc cgtcgcagca
      601 atcgatgacg gccgacagct ggaacaacac cgccgcgatg acacccgcct ggtaggagcc
      661 gaaggcgaag caggccgccg agatgaggcc gatccccgtg gctaccaggg tgaccgcatt
      721 gggcgagaaa ccaaaagcca agaacacgcg cgtgaaccag tgcgagagtt tgcgattgaa
      781 gaagcgatcg acgaaccctt cgtattcgcc cttgagcgag gagaagagtt tgcgctcagc
      841 aagttggaca tcggcgtgat cgcgcaggtc ctgataccaa tgggagggat tcgactccgt
      901 gctgagcacg cgcacgcgtc cctcttccgc agcccgttcg agccaccggc gaagcggcac
      961 ggtcccgctc tcaatcccct ctcggctcac ggcactgatg aagccggacg ggagtacgat
     1021 cacgtctgtg gcgaccaagg cagcatcatc ctgtcttgac gattcgagcg acaggagccg
     1081 ttcgccttgt gccgtgatac gcaaccccag acgccgttcc tgttgatccc gcaccgtgga
     1141 ggcccgtgcc acgaccatca cttggccctc ctgtacttcc cggcgtaaat gctcgatcag
     1201 ctgccgtgag aagacccctc gcactccgga aaccagacag aatccacccg actcggcggc
     1261 gagcgattcc cacgtgcgcg aatcgtctaa cggaaattcg cgaatcggca tccagcggac
     1321 cggaatcgtc aggcgcggcc ctttccccaa tgcctgcttc aattgatctt cctccgaccc
     1381 gaccagcacg atcagctgcc ggatcccggc ccgttgcagc gtgagcaccg tgcgctggaa
     1441 gagcccgatg ccaaccacca gcgtcagcgg accgacgcgc cgtaccggca agcggcctga
     1501 ttcgtccagg agatcgattg agggcagcag gatggccgtg gccagtccct gcacttccgc
     1561 tcgtttctgg atgagacttt cgctcatatt acgaccttaa attttcggca ggatttctcg
     1621 ttccgctttc gcgacgtctt ccgtaaaatc gatctcggtc caggcgagcc cgccgatggt
     1681 ttcgtacccg accttcacgt tcttgaaaaa ctccagcaga gcatcttcgt attccatgtg
     1741 ccaggcttcg cggtccacat aggttctcaa cgaagccacg acgtgcgggg tatcggcgtg
     1801 ccggaccttc aaaaacccca ctccttcgcc ggccacatca taccggagcg gcatcttctt
     1861 tgtcagcgcg atcacccgcc cgccttccgc gaccaccata cattcttcgc cggtctgctt
     1921 cacggtgtca tccatcaaga ggcaattcgg ccagggcgac tgcaccaacc gtctcaaaat
     1981 ttcacggtcg aagagcacgt cggcatccat gatgatgacg tcatcgtcca acacggtgcg
     2041 tgcaatccag agcgacgaga tgctcccgcg atggaattgc tcgttgacca gaaagttcac
     2101 gcgcacgcca cgggaatcag cttccacggc ggaccgaatc atctcctgct tgtaccccac
     2161 cacgatatcc gcgcgctgaa tgccgaggct tgccaacgcc gtcaaatagc gatggagcag
     2221 cgtctgcccg ccgatctgaa tgaggcactt gggacgatgt tgggtgaccg gccagagacg
     2281 cttcccgacg ccggccgcca ggatgatggc tttcatgcgg cggtgcagat gcgttcgaag
     2341 gcgtcgagga atccctggat ctgagcctgc gacagcactc ccatgttggc gatgcggaag
     2401 atctgccctt ccaggttgcc ctgaccggca tagatcacgt agccctgttc tttcaacgcg
     2461 tcgtgaagcc tggcgtaact gagacccggc gggagatgat aggccgtaat cgtattggac
     2521 tggtaatccg gtggcaacag cggcttgatg ccgagcttcg ccatgcggtc ccgaatgaaa
     2581 gtcgagatgt tcctgtatcg ctgaatgcgc ttggcgaccc cttcttccag taattcgctg
     2641 agcgcctcgt caaaggcata gtagagttgc acagccggcg tgaagggaac cgtgccgaga
     2701 ccttgatcat cgacatagtg cgtcatatgg agataccaag tccgcttcgg atatttccgc
     2761 atccgctcga ggaacccctt gcgcacgagc acaaacgaca cgccggggaa tccctggatg
     2821 cacttacccg ccgtgccggc cgccatatag atgtgcggcc cgacgatatc gagcgtttcc
     2881 ccagccatcg cgctgaccgc atcgaggatg aacacgcggt tctggctgtc aacaacttcg
     2941 gcgatctctt tgacaggatt gatgaggccg gtcgtcgttt cgtgatggac catcgccacc
     3001 gcatgcactt ccggatgttg tcgcagcgcg aggcggagtc gttccggatc gggacgggca
     3061 gtccagtcgt acttgagttc cgagacaccc aaccgattca atccgacaat ctgtgaaatt
     3121 cgctcaccgt agactccatt gttcaacacc agcatgcgtt tgccgtgagg gatcgaggac
     3181 attaacgctg attccacggc ggaggtcccg gacccggtga tgaccacggc cgcatagtcg
     3241 gattccgcgc ctggcacgaa cgcctgcaag agcttggctt gaatgcggtg gagcaagtcg
     3301 gtgaattccg tctcgcgatg gcagatgtca gggcgcaaca atgcttgacg cacgcgctcc
     3361 gacacattca ccggccctgg attcaataag atcatctgtg ttcgtttcct tcctgtaccc
     3421 cgtagacgcg aacggtttac tcaatcgctt tcatgaaccg tttcgtaata tccggcggat
     3481 cgagcacgac cctgcccgca tcttcagcca attcgttgac cttgatgagc aacatgctcg
     3541 ggccgtcttt cttgagcatg tctttaaatt cgtacacgag atct
//
LOCUS       DATDNW010000285         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_246041, whole genome shotgun sequence.
ACCESSION   DATDNW010000285 DATDNW010000000
VERSION     DATDNW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246041"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VI359_06845"
     CDS             complement(<1..1276)
                     /locus_tag="VI359_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY6974004.1"
                     /translation="MPALRLKQFAVAALLSLLVGSLLSAAFLSGLFANAQLRGTDFLF
                     TDQPESFSGAIVIVGIDQRSYRELLPRHGPLVRWSRALYAQAVDRLRSAGARVIALDI
                     FFDAPGAEDSGLIDAISRAGNVVFPVEGQGPGAFHPRPGVVQVFDVFVRATPRIAAAA
                     VAEGSVNVTTDRDTVVRSLPLLLESGEGLLPSLSLAIISRFIRRANILDQDPTSVMIY
                     AAGRTIPVIDNGRMLINYSGPPSSPGVSRSFPIIPFVDVISGSFDENLVRDKIVLLGL
                     TIRGIDEFATPTTSTTGMWGVEVQASAVDTLLAERYLLPASRTTTVLLIYAAALTGAI
                     FAAAARPLNGIGSVAASLFLYLVGASMSFDRGVLLNMVYPPAAVLLGFTATQGYRIFF
                     EQAQRQLVQDLMSRYLSPSVSQWVLQQPEQLKL"
     gene            complement(1493..1568)
                     /locus_tag="VI359_06850"
     tRNA            complement(1493..1568)
                     /locus_tag="VI359_06850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1533..1535),aa:Lys,seq:ttt)
     gene            complement(1634..>2009)
                     /locus_tag="VI359_06855"
     CDS             complement(1634..>2009)
                     /locus_tag="VI359_06855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY6974005.1"
                     /translation="GVVARYATMVNGMTSLAVTKLDVLDGCKELKLCTGYRYNGRVFR
                     DMPADLHALTHCEPVYRTFKGWTAPTTGVRTYKGLPSEAKRYLQAIEDLAECRIDMIS
                     TGSKRGETIVLRNPLKARSRAR"
BASE COUNT          451 a          568 c          633 g          357 t
ORIGIN      
        1 cgagcttgag ctgttccggc tgttgcagca cccactggct gaccgaaggc gagagatagc
       61 gggacatcag gtcctgaacc agttgccgtt gagcctgctc gaagaaaatc cggtagcctt
      121 gagtggccgt gaatcccagc agcaccgcgg ctggcggata gaccatgttg aggagcacgc
      181 ctctatcgaa cgacatgctg gcgccgacca gatagaggaa caacgatgcc gccacgctgc
      241 caattccgtt gagcgggcgc gcggcggcgg cgaagatggc acccgtcaat gccgcggcat
      301 agatcagtaa aacagttgtc gtccgggacg cgggcagcaa atatcgttca gctagtaatg
      361 tgtcgacggc gctcgcctgg acttctacgc cccacatgcc ggtcgtggat gtcgtcggcg
      421 tggcaaactc atcaataccg cgaatggtca ggcccagcag cacgatcttg tcccgaacga
      481 gattctcgtc gaatgatccg ctgatgacat ccacaaacgg tataatcgga aagcttctgc
      541 tcaccccggg agacgacggc ggaccggaat aattgatcag catccgtccg ttgtcgatca
      601 ccgggatcgt cctaccggct gcatagatca tgacgctcgt ggggtcttga tccagaatat
      661 tggctcgtcg gatgaagcgg gagatgatcg cgagggaaag ggaaggaagc agcccctcgc
      721 ccgactccag caggagcgga aggctacgga cgacggtatc gcgatccgtc gtgacattga
      781 cggatccctc ggcgacggcc gccgccgcaa tgcgcggcgt agcccggacg aagacgtcaa
      841 agacctggac aacgccaggg cgggggtgga aggcacccgg gccctggccc tccacgggaa
      901 agacgacgtt gccggcgcgc gagatggcgt caatcaggcc ggagtcctcc gctccgggcg
      961 catcgaaaaa gatgtcgagg gcgatgacac gggcgcccgc agaccgcaac cggtcgaccg
     1021 cctgtgcata gagcgcccgc gaccatctga cgagaggccc gtgtcggggc agcagctctc
     1081 gataactccg ctggtcgatg ccgacgatca caatggctcc ggagaaactt tcgggctgat
     1141 cggtgaagag aaagtcggtg ccgcgcaatt gagcgttggc gaacagccca ctgagaaacg
     1201 cagcgcttaa gagactaccc accaggagcg agagaagggc agcgacggca aattgcttca
     1261 ggcggagagc tggcatcgcg ccattttaga aaaccaggtc cgtccgatca atcggggggc
     1321 tttatcggca ggatgggtgc ccagatgagg actcccttgc tttagaaggc agctgttcct
     1381 cgcgacaaag ttcaaccacc atgcgcgatt ttgatagatg ggtgatcgtg accaaaatcg
     1441 tgaccgaaga aacggtcacc gacgaagaga cgcagaagtt gttgaataag ggtggtgagc
     1501 cgcctgggac tcgaacccag ggccctcgcc ttaaaagggc gatgctctac cgtctgagct
     1561 agcggctcac cgaatggaga gtggggattc taccatcgat gatcaatgtg tgcctagaga
     1621 gcacgttgat gacctagcgc gctcgcgagc gagccttgag gggattccgc aagacaatgg
     1681 tctcgccacg cttcgaaccg gtcgagatca tgtcgatccg acattcggcc agatcttcga
     1741 tggcctgtag gtaccgtttg gcttcagacg gcagtccttt ataagttcgt acgccggttg
     1801 tgggagcggt ccagcccttg aatgtgcgat agaccggctc acagtgggtc agcgcatgca
     1861 gatcagcggg catgtctcga aagacccggc cgttgtagcg atatcccgtg cagagcttca
     1921 attccttgca cccgtccaag acatcgagct tcgtgacggc gagcgaggtc atgccgttca
     1981 ccatggtggc gtagcgtgcg accactcca
//
LOCUS       DATDNW010000286         3198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1335363, whole genome shotgun sequence.
ACCESSION   DATDNW010000286 DATDNW010000000
VERSION     DATDNW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335363"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1314)
                     /locus_tag="VI359_06860"
     CDS             complement(28..1314)
                     /locus_tag="VI359_06860"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY6974006.1"
                     /translation="MTILIRGGQVIDPGRLNAVADILIEDGKIAAVGRNLTAPRGATI
                     IEAQDRLVLPGFVDLHVHFREPGFEYKETIQSGAAAAVAGGFTTVCCMPNTNPVNDNQ
                     AITEFILDRAREAGLANVLPIGAVTKGSEGKELAEIGDLRRAGCVAISDDGRPVMNSL
                     VMRRAMEYALAFDVPVIDHCEDLHLAEGGCMNEGLVSTELGLPGIPAAAEDVMVARNV
                     ALAELTGARLHLAHISTQGSVRMVREAKSRGIKVTAEACPHHFTITEEVVRGYNTHAK
                     MNPPLRTWKDVQAIKEGLRDGTIDAIATDHAPHATQEKQQGFTDAPFGIVGLETAWPL
                     TFALVEEGILSVESAVERLSTAPAKAFGLSKGTLAVGADADVVVADPQESWEVDPAKF
                     RSKSRNTPFAGWKVKGRIKTTIVGGRVVFETSQVER"
     gene            complement(1318..2238)
                     /locus_tag="VI359_06865"
     CDS             complement(1318..2238)
                     /locus_tag="VI359_06865"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY6974007.1"
                     /translation="MSLKRKDLLSLAPLSVDEIRLMLETADSFKEVTGREIKKVPALR
                     GRTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSASSVVKGETLLDTARNIEAMQA
                     DIIVLRHPSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRERGLSFEGLRVA
                     IVGDVAHSRVARSNIHALTKLGAEVRLVGPPTMMPWGVEKLGARVYYNLDEALQGVQV
                     IMMLRLQLERQGRALFPTIREYARLYGLTAERVKIADSGAIVMHPGPINRGVEIAPEV
                     ADSLSSVILDQVANGVAVRMGILYLMSGAN"
     gene            complement(2252..2827)
                     /gene="pyrR"
                     /locus_tag="VI359_06870"
     CDS             complement(2252..2827)
                     /gene="pyrR"
                     /locus_tag="VI359_06870"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY6974008.1"
                     /translation="MPTISMTTTAHSDRQQERLVMDAGDIARALTRIAHEILERNKGV
                     HGLALVGIRTGGVYLAHRLVRRIQDIEGVIIPIGELDITLYRDDLSLRKEQPILRKTS
                     VPFDISEKIIVLVDDVLFTGRTIRAAMDGLIDLGRPAEIQLAVLVDRGHRQLPIKASY
                     IGKNLPTSRDEKVQVMLEEDGEDDRVVILKA"
     gene            complement(3013..>3198)
                     /locus_tag="VI359_06875"
     CDS             complement(3013..>3198)
                     /locus_tag="VI359_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974009.1"
                     /translation="QPNGHAFLVGFRELGEHLIQGGLSLRWQEGVENLPTFRMHRSIL
                     PKARLRNGLTLFHVTTA"
BASE COUNT          678 a         1024 c          791 g          705 t
ORIGIN      
        1 ccccagcggg atccacgcat tgcccttcta ccgttcaacc tggctggttt cgaacaccac
       61 gcgacctccg acgatcgtcg tcttgatccg ccctttgacc ttccagcccg cgaacggcgt
      121 atttcggctc ttcgaccgaa acttcgccgg atcgacttcc cacgactcct gaggatccgc
      181 cactaccaca tccgcgtccg cccccaccgc caacgtacct ttcgacaacc cgaatgcctt
      241 cgctggagcg gtgctgagtc tctcgaccgc agattcgacg gataaaatcc cctcttcgac
      301 cagagcgaac gtcaacggcc atgccgtttc caatccaacg attccgaagg gcgcgtcggt
      361 gaatccttgc tgcttttctt gcgtggcgtg aggcgcatga tccgtggcaa tcgcgtcaat
      421 cgttccatcg cgcagtcctt ctttgatcgc ctgaacgtcc ttccaagttc tgaggggcgg
      481 attcattttg gcatgcgtgt tatagcctcg gaccacctcc tccgtgatcg taaagtggtg
      541 ggggcaggct tctgccgtca ccttgatgcc gcgggacttt gcctcacgca ccatccgcac
      601 cgatccttga gtgctgatat gggcgagatg aagccgcgcc cccgtcagtt ctgcaagggc
      661 cacattgcgc gcgaccatca catcctcggc tgccgccggg atacccggga gccccaactc
      721 tgtcgacacc agtccttcat tcatacaacc gccttcggcc aggtgcaaat cctcgcaatg
      781 gtctatcact ggtacatcga acgccaatgc atactccatg gcacgtcgca tgacgaggct
      841 gttcatcacc ggtcggccat catcggagat cgccacgcag ccggcacggc gcaaatcacc
      901 gatctcagcc aattctttgc cttccgagcc cttggtaacg gctccaatcg gcaagacgtt
      961 cgccagtccc gcctctcgag cccgatccag tatgaattcc gtaatcgctt gattgtcgtt
     1021 caccggattt gtattcggca tgcaacagac tgtcgtaaat ccaccggcga ctgctgccgc
     1081 cgccccgctt tggatcgttt ctttatattc aaagccgggt tctcggaaat ggacatggag
     1141 atcgacaaag cctggaagca ccagtcgatc ttgtgcttcg atgatcgtcg ccccgcgagg
     1201 cgcagtgaga ttccgaccaa ccgccgcgat cttgccgtct tcaatgagaa tgtcggcaac
     1261 cgcattgagc cgcccgggat cgatgacctg tccgccgcgg attagtatgg tcacgattta
     1321 gttggctcct gacatcagat agaggatgcc catgcgcacg gctacgccat tggcaacttg
     1381 gtcgaggatc actgaggaca gactatcggc aacttccggt gcgatctcga ccccccgatt
     1441 gatcgggccc ggatgcatga cgattgctcc agagtcggcg attttcaccc gctccgccgt
     1501 taacccgtac aaccgagcgt actcgcggat cgtcggaaac aacgcacgcc cctgtcgttc
     1561 caattggagt cgcaacatca taatgacctg cacgccctgc aacgcctcat ccaagttgta
     1621 atagacgcgg gcgcccagct tttctactcc ccacggcatc atcgtcggtg gaccaaccaa
     1681 ccgaacttcg gcccccagtt tggtcagcgc atgaatattc gaacgagcga ctcggctatg
     1741 ggcaacatcg cccacgatcg cgacccgcaa tccctcaaac gacaagcccc gctcacgaat
     1801 cgtgtagaga tcgagcaacg cttgagtcgg atgctcatgc cagccgtcgc ccgcattgat
     1861 caccgacgac ttcactccgc gggcgagggt ctcagccgca cctgctgacg ggtgacgcaa
     1921 cacaataatg tccgcctgca tcgcttcgat gttgcgggcc gtgtccaaca acgtttcgcc
     1981 ctttacaacg ctgctcgccg acggcgagaa gttgatgacg tctgcgctga gccgttttgc
     2041 cgccaactca aacgacgtgc gggttctggt gctcggctca aagaataaat tcacgacagt
     2101 tcggccccga agcgccggga cttttttgat ttcacggccc gtgacttcct tgaaagaatc
     2161 agcggtttca agcatcaacc gaatctcatc aaccgatagc ggggcaagac tcaaaagatc
     2221 tttacgtttg aggctcatcg tgtcctcccc ttcaggcctt cagaatcacg acacggtcgt
     2281 cttcgccgtc ttcttccagc atgacctgca ctttttcatc tcgcgatgtg ggcaagttct
     2341 tcccgatata actggcttta atcggtaatt gacgatgacc acgatccacc agcacggcca
     2401 actggatttc ggccggacgt ccgagatcga tcaacccatc catcgcggca cgaatcgtcc
     2461 gtccggtaaa cagaacatca tccaccaaca caataatctt ctccgagata tcgaatggca
     2521 cggaggtctt ccgcaaaatc ggctgctcct tccgcaagct caggtcgtct cgatacagcg
     2581 tgatgtccaa ctcgccgatc gggatgatca ctccttcgat gtcttgaatg cgccgtacca
     2641 agcgatgtgc caaatagacc ccgccggttc gaatcccaac cagggccagg ccatgtacgc
     2701 ccttgttccg ctccagtatc tcgtgtgcga tgcgagtcaa ggctcgcgca atatcccccg
     2761 catccatcac caaccgttcc tgctgtcgat cgctgtgcgc cgtggtcgtc atcgaaattg
     2821 tgggcataaa aaaaccttcc cctccaccat ggaagagaag gttgaccgga actcgcgcgg
     2881 acgggattga ctgcgatgca tcgtccactc cttgctcacc tcgcaggatg agctttaaag
     2941 gttatgacat cttaacgagg cggtgccgat tctgtcaagg caatgggagc aaccaccaca
     3001 tggatccgcg cgttaggctg ttgtgacgtg aaagagcgta agcccattcc tgagccgtgc
     3061 cttcggcaga atactccgat gcatccgaaa tgtcgggaga ttttcaactc cttcttgcca
     3121 acgaagcgac aatcctcctt gaattaaatg ctcaccgagt tctctgaaac ccacgagaaa
     3181 ggcatgtccg tttggttg
//
LOCUS       DATDNW010000287         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_584481, whole genome shotgun sequence.
ACCESSION   DATDNW010000287 DATDNW010000000
VERSION     DATDNW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584481"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VI359_06880"
     CDS             <1..770
                     /locus_tag="VI359_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY6974010.1"
                     /translation="GPPVILIHGFGGSMWQWEHQQTVLSTKFRVITPDLVGAGLSDKP
                     EIDYRPDELLAFLVGFMDALQIRRADVVGNSMGAGLAIGMALDYPTRVSNLVLIDGFP
                     ARVMDHLSSPTVRRALETSAPAWVVSFGNWLFGWAMTESILREFIYDPALITPAVLDR
                     SNRNRRRPGQFRSLLTIGQNLPVWEEQFAPRIGKVTQRTLIVWGEEDRVFPVKAGETL
                     NQTIPGSRLIRIPRAGHIPQWEQPDLVNAELLRFLQS"
     gene            841..1494
                     /gene="tpx"
                     /locus_tag="VI359_06885"
     CDS             841..1494
                     /gene="tpx"
                     /locus_tag="VI359_06885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEY6974011.1"
                     /translation="MADHQWTLTVALLILLLNAGCGSTGESMFLYKNLPVASGTVIAG
                     DGHTVLFKGSPSALSGTGVTIGDPLRDVKVAQTDLSLINVVETKGKGKVRIISVVPSL
                     DTKVCEQQTHYLSEKNKGLDKMVELITISIDTPFAQKRFAEEAKIDNVTFLSDYRGGD
                     FGKAHGLFLKGPHILARAVMVVDAQNKIRYLQITPELGQLPNMDEAFTFTRSLITAS"
     gene            1496..>2835
                     /gene="sthA"
                     /locus_tag="VI359_06890"
     CDS             1496..>2835
                     /gene="sthA"
                     /locus_tag="VI359_06890"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEY6974012.1"
                     /translation="MAHYDLLVIGTGPAGQKAAVQGAKLGKKVGIIERKEVVGGVCIN
                     TGTIPSKSFREAILYLSGFRQRNFYGADYRLKQTITIVDLAFRCQHVIANEIQIVQNQ
                     MTRNRVDMLYGTASFVDPHRLRIQQTTGSVEHTANFIVIAVGTEPAKPSHVPFDVASI
                     IDTDGILTLKRIPTSMVIVGGGVIGTEYASILATMGVSVILIDKRPRLLEFMDAEIIT
                     TLQQQMKDIGVALYHDEEAVAIRKDSDGQVHVTLKQARAVTAETLMYAIGRVGATSDL
                     NLPAVGIIPDDRGRLTVNEHFQTSVSHIYAAGDVIGFPALASTSMQQGRHASCHAFGH
                     PDRTDTALLPYGIYSIPEMSMVGRTEEDLTKADVPYGVGIARYREIARGQLIGDDRGM
                     LKLLFHRHTREVLGIHAIGEGATELIHIGQAVMAYRGKIEYFIDTVFNYPTLAE"
BASE COUNT          638 a          834 c          764 g          599 t
ORIGIN      
        1 aaggtccacc ggtgatcttg attcatggat tcggcggctc gatgtggcag tgggaacacc
       61 agcaaacggt cctttccacg aagttccgag tgatcactcc cgatctggtt ggggcggggc
      121 tctccgataa acccgagatc gactatcgac ccgacgagct gctggccttc ttggtgggat
      181 tcatggacgc gcttcagatt cgtcgagccg atgtggtcgg caattcgatg ggcgctggac
      241 tcgccatcgg aatggccctc gactatccca cccgtgtctc gaacctggtg ctcattgacg
      301 gctttcccgc ccgcgtgatg gaccatctca gcagtcctac ggtccgccgt gctttagaga
      361 ccagtgcacc agcgtgggtc gtctcgttcg gcaattggct cttcggatgg gccatgaccg
      421 aatccatttt gcgggaattc atttacgacc ctgccttgat cacacctgcc gtgctggatc
      481 ggtcgaatcg caaccgtcgc cggccaggac agtttcgctc tctcctgaca atcggccaga
      541 atcttccggt ctgggaagag cagttcgcgc cacgcatcgg taaggtgacc caacgcacgc
      601 tgatcgtgtg gggcgaagaa gaccgagtgt ttccggtcaa ggcgggtgag acccttaatc
      661 agacgattcc tggctcgagg ttgattcgga tcccccgagc cggtcacatt ccgcaatggg
      721 agcaaccgga cctggtgaat gcggagttgc tcagatttct tcaatcctaa gaagtcattc
      781 cgattcccca tttatgtcgt acaatggaca ggccgtcggc acaatacgaa ggaggaaatc
      841 atggccgatc accagtggac gctaacggta gccctcctca ttctcctctt gaacgcaggg
      901 tgtggaagca cgggagagtc gatgttcctc tacaagaatc ttcctgttgc aagcgggacc
      961 gtcatcgctg gagacggcca tacggtgttg ttcaaaggca gcccgtccgc tctctcggga
     1021 acaggtgtga caattggcga cccgttacgg gatgtgaaag tcgcccaaac cgacctctcg
     1081 ttgattaacg tcgtcgagac caagggcaaa gggaaggtcc gtatcatcag cgtcgtcccg
     1141 tcgttggata caaaggtctg cgagcagcag acgcactatc tcagtgaaaa gaataagggg
     1201 ctcgacaaga tggttgaact catcacgatc agcatcgata ccccgttcgc ccaaaagcgt
     1261 ttcgccgaag aggcgaagat cgacaacgtg acgtttctct cagactaccg tggtggcgac
     1321 tttgggaaag cccatggact ctttctgaaa ggcccccaca ttctggcccg tgcggtcatg
     1381 gtcgtcgatg cccagaataa aatacgctat cttcagatta ctcctgaact tgggcagcta
     1441 ccgaacatgg atgaagcctt caccttcacc agatcattga tcacggccag ctgatatggc
     1501 gcattacgac ctcctcgtga tcggaacagg accggcggga caaaaagcag ccgtgcaggg
     1561 tgccaagctc ggcaagaaag tgggcatcat cgagcggaaa gaagtcgtag gcggcgtctg
     1621 catcaacacc ggcaccattc cgagtaaatc atttcgcgaa gccatcctgt acctctcggg
     1681 gttccgccag cgtaatttct acggggcaga ttaccgcctc aagcaaacca tcacgatcgt
     1741 agatttagcc tttcgctgcc aacacgtcat tgcaaacgaa attcaaatcg tgcaaaacca
     1801 aatgacccgg aaccgggtcg acatgctcta cggcaccgcc agcttcgtcg atccccaccg
     1861 gctccgtatt caacagacga ccggttcagt cgaacatacc gccaacttta ttgtcatcgc
     1921 cgtgggcacg gagccggcga aaccctccca cgtgcccttt gacgtcgctt ccatcatcga
     1981 tacagacggc attctcacgc tcaagcgcat ccccacatcg atggtcattg tcggtggtgg
     2041 cgtgatcggc accgaatatg cgtcgatcct cgccacgatg ggagtctcgg tcattctcat
     2101 cgacaaacga ccgcgactcc tcgagttcat ggatgcggaa atcatcacca ccctccaaca
     2161 gcagatgaaa gacatcggcg ttgcgctcta tcacgatgaa gaagccgtcg ccattcgaaa
     2221 agattccgac ggacaggtcc acgtgacgct gaagcaggcc agagcggtca cggccgagac
     2281 cttgatgtac gccatcggac gtgtgggagc gacttcggac ctcaatctgc ccgccgtcgg
     2341 catcatccct gacgaccgcg gccgcttgac ggtgaacgaa cattttcaaa cttcggtctc
     2401 gcacatctat gccgcaggag acgtcattgg ctttcccgcc ttggcctcga cctccatgca
     2461 acaaggccgt catgcttctt gccacgcatt tggtcatccg gatcggaccg acaccgcatt
     2521 gttaccgtac ggcatctatt cgatcccgga gatgtcgatg gtgggccgaa cggaagagga
     2581 tctcaccaag gccgatgtgc cctatggagt gggaatcgcg aggtaccggg aaattgctcg
     2641 cggacaactc atcggagatg accgcggcat gttgaagctc ctcttccatc gtcacacccg
     2701 cgaggttctc ggcatccatg caatcggtga aggagccaca gaattgattc acatcggcca
     2761 agccgtcatg gcctatcgcg gcaagatcga gtacttcatc gatacggtct tcaattaccc
     2821 cacgttggcc gagtg
//
LOCUS       DATDNW010000288         7179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_313883, whole genome shotgun sequence.
ACCESSION   DATDNW010000288 DATDNW010000000
VERSION     DATDNW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313883"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="lipB"
                     /locus_tag="VI359_06895"
     CDS             <1..697
                     /gene="lipB"
                     /locus_tag="VI359_06895"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY6974013.1"
                     /translation="SAAVFEFAEPVPYADAWNLQKRLHEERVVDHRPDTLLLLEHVPV
                     YTAGRTTQASHWSGEGKNLHPAGLEIHAVDRGGSLTYHGPGQLVGYPIIRLARYASGP
                     RQYVRLLEDVIIDTLRVWKIEGYRVDKKPGVWVQLDQQEAKIAAIGVRIERGVTLHGF
                     ALNVEMDLSPYSRIVPCGLAGCRVTSMAEAIGSTVSLPAVRSAMQRTFSHTFNLEWTS
                     IKSGLLTETIATR"
     gene            701..1957
                     /locus_tag="VI359_06900"
     CDS             701..1957
                     /locus_tag="VI359_06900"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY6974014.1"
                     /translation="MNELDTPTFRLAVAQFDRAAEAMNLDQNLRERLKLPQRSLVVSL
                     PVRMDDGRVEVFTGYRVQHDSSRGPSKGGIRYHPDVNLGEVAALAMWMTWKCALADLP
                     YGGAKGGVKVSPKRLSRAELQRLTRRYAAEIFPLIGPEKDVPAPDVGTDAQVMAWIMD
                     TYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVYITLEALRHLKIDVRNTTVAIQ
                     GFGNVGSHTALIMRECGARVIAVSDVTGGLYNPAGLNISELLRRYKTAGEPLKDTKMG
                     DAITNDDLLQLDCTVLVPAALSEQITKRNAAQLRCRVLAEGANGPTTLEADQILEDKG
                     VFILPDILVNSGGVIVSYFEWVQDVQRFFWKAKDIQERLQEIQSSAFHRTLQFATTQK
                     TSMRMAALMSGIDKVAQAHLQRGLYP"
     gene            2021..2407
                     /locus_tag="VI359_06905"
     CDS             2021..2407
                     /locus_tag="VI359_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974015.1"
                     /translation="MGDYALTAIAGIWFADGLLLLLVPRFIITHFRQTLAESPTILRW
                     EWLAVAGGGIVLIAGQHLHYQPLWIITAGVMIVKGLFLAMGPSTWRTRVLDWCLSRED
                     VDYRFWGLGLCSLATLLLHALGWISG"
     gene            2436..2729
                     /locus_tag="VI359_06910"
     CDS             2436..2729
                     /locus_tag="VI359_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974016.1"
                     /translation="MTRHTIVSLWEAHKVERWPRLDSHQEGPLMTLDTVISGCVVYYL
                     DSPDGLDAQRRTIVGDCLADLDIFAEELDEDSRQYFQRLRHLGSLLLASSDPD"
     gene            complement(2782..3234)
                     /locus_tag="VI359_06915"
     CDS             complement(2782..3234)
                     /locus_tag="VI359_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEY6974017.1"
                     /translation="MRSFRRTSTGLVGAGIFLALLGCAERGPIPAGKSLYDRLGGKTA
                     IAAVIDQFVANVAADTRINGRFATTDIPKLKGHLVDQVCVATGGHCTYAGRDMKTTHA
                     GMKISNADFGALVEDLIKALDTFKVPPREKEELLGLLAPMKKDIVEVP"
     gene            3419..4354
                     /locus_tag="VI359_06920"
     CDS             3419..4354
                     /locus_tag="VI359_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY6974018.1"
                     /translation="MELRHLRYFIAVASAQNFTKAAAAVHVSQPSLSVQVRALEDELG
                     ARLFDRLGRKVTLTQAGELFRDHAERAVRELERATQLVQEVHGAQRGRLVVGTLATVN
                     SYLIPPLVSQFKQRFPGIHLQIHSLPSDDIVSSILANRLDLGICLLPLVHGRLSTVPL
                     FEERLVLVAPRDYPLRQSRLRMKDLAGLPLVLMPADYCLRKMVEAECTEARVKTDVVL
                     EMTSPEGILQAVAEGAGLTILPELYVRLRSPGARFRLIELYDPVPRHSVGLVSLANRY
                     QNLAAKEFATLCRTTMARLMTKSQSSSSRRHLGRA"
     gene            4536..5069
                     /locus_tag="VI359_06925"
     CDS             4536..5069
                     /locus_tag="VI359_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974019.1"
                     /translation="MRIMVRVVMLSSLMAAVLVGWVIPSYGEPYELSKNDVMDPKTVK
                     SADIALFGVKLGDPEAKALDVLVNEKIPGIKVEQEALFIFLLDQRKPTGPMAGVRIQD
                     GKVDLIFINNRFAYKTRGIFRNVLNSESPEDVRKLLGQEEYGDENVMGAVLAYDKQGF
                     VVNYLGKDVNVEFSTQR"
     gene            complement(5229..6098)
                     /locus_tag="VI359_06930"
     CDS             complement(5229..6098)
                     /locus_tag="VI359_06930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY6974020.1"
                     /translation="MCALILLVLWNLTIFIPSAALAQKDRISAPQAHTILADGRPLPW
                     RKVPAHSILLKELNSGRVLYEHESGKRLSPASLTKIMSALVILEQGRLDDQVTVSKNA
                     ARAHKTHLRLKPGQVFRLEDLLKAMLIVSANDACLAAVEHVGGDEDQFVTLMNAKAAA
                     LGLSDTHFSNACGFDGVDHYSTAEDLAQLSLTALEQPTFRQLVREEHSIIVPVNGYRP
                     YVLHNTNRLLGRIPGVEGVKTGFTSKAGRCLIAKVSQNGSDLLLVILNSNRRWDTAKN
                     LINFGLQANSIVH"
     gene            complement(6157..6345)
                     /locus_tag="VI359_06935"
     CDS             complement(6157..6345)
                     /locus_tag="VI359_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974021.1"
                     /translation="MDCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVIDSGFRGSKVRESFTL"
     gene            complement(6370..6561)
                     /locus_tag="VI359_06940"
     CDS             complement(6370..6561)
                     /locus_tag="VI359_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974022.1"
                     /translation="MRSFAWWFTIGALLTLSVLMVQGGVRDLFDGTHSQSGYLSFGTT
                     ILGGGLLAGCVALVMNRIR"
     gene            complement(6696..>7179)
                     /locus_tag="VI359_06945"
     CDS             complement(6696..>7179)
                     /locus_tag="VI359_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY6974023.1"
                     /translation="TFRGPTDYRSGKRPLGISFADFNDDRVQDMLVINGERDSFTTFL
                     GKGDATFQAGRDSGADAGPNFGLARDFNGDRHADVAIVNLQSSDLSILFGRGDGTFEY
                     PPKNYRTKAGPFAVAVFRVTTKEAEEPGLVTADNGNGSVSIFLHRGLKSGRVSASDAR
                     "
BASE COUNT         1632 a         1986 c         1915 g         1646 t
ORIGIN      
        1 gtcagcagcc gtattcgaat tcgccgagcc agttccttat gctgatgcct ggaaccttca
       61 aaagcggctg cacgaagagc gggtagtcga tcatcggccg gacacgctgc tcctactcga
      121 gcacgtaccc gtgtataccg ccggtcgtac aacgcaggct tcgcattgga gtggggaagg
      181 gaaaaatctc caccccgctg gtctagagat ccacgctgtc gaccgcggag gctctcttac
      241 ataccatgga cctggacaac tggttgggta cccgatcatc cggcttgctc gctatgcctc
      301 aggaccaagg caatacgtcc gattgctcga agacgtgatc atcgatacgc ttcgagtatg
      361 gaaaattgaa ggctatcgcg tcgacaagaa gccaggagtg tgggtacagc tggatcaaca
      421 agaggcgaag atcgcggcca tcggagtccg gatcgaacga ggcgtgaccc tccacgggtt
      481 cgccctcaat gtcgagatgg acctgtctcc ttattctcga atcgtgccct gcggcctcgc
      541 aggttgtcgc gtgacctcga tggcggaagc catcggttcg acggtgtcgc tccccgctgt
      601 gcggtcggcc atgcaacgaa ccttctcgca cactttcaat cttgaatgga cctccataaa
      661 gagtgggctg ctcacagaga ccatagcaac gaggtaaacc atgaatgaac tcgacacgcc
      721 gacgttccga ctcgccgtgg cgcaattcga ccgcgcggcg gaagcgatga acctcgatca
      781 gaatctgcgc gagcgcctga aacttcctca gcgatcgttg gtcgtcagcc ttcctgttcg
      841 tatggatgac ggacgcgttg aagtcttcac cggttatcgc gtccagcatg actcgtcgcg
      901 cggcccttca aagggcggca tccgctatca ccccgacgtg aatctcgggg aagtcgccgc
      961 tctcgccatg tggatgacgt ggaaatgcgc gctggccgat ctgccgtatg ggggggctaa
     1021 gggtggcgtg aaggtttcgc ccaaacggtt atctcgagca gaattgcaac gattgacccg
     1081 tcgctatgca gcagagattt tccctctgat tggcccagaa aaggatgtgc ccgcccctga
     1141 tgtcggcact gatgcgcagg tgatggcatg gatcatggat acgtatagcc agcaagtcgg
     1201 ctatgccgtt cccggtgtcg tcacaggcaa gccgctgtcg atcgggggaa gtctggggag
     1261 agaagaggcg accggtcgcg gagtggtcta cattacactc gaagccctgc gccacttgaa
     1321 gatcgatgtc cgaaatacca cggtcgcgat tcaaggtttc ggtaatgtcg gctcgcacac
     1381 ggccctcatc atgcgggaat gcggcgcccg tgttattgcg gtcagcgacg tgaccggggg
     1441 gctttacaat ccagcggggc tcaacatttc ggaactactt cgtcgataca agacggctgg
     1501 ggaaccgctc aaagacacga agatgggtga tgcaatcacc aatgacgacc tgctccaact
     1561 cgattgtacg gtcctagtgc ccgccgcgct ctccgaacaa attaccaaac gcaacgcggc
     1621 gcagcttcgc tgccgtgtgc tggctgaagg agcgaacggc ccgacgactc tggaggccga
     1681 tcagatcctt gaagacaagg gtgtctttat tctccccgat attcttgtca actcgggtgg
     1741 tgtgatcgtg tcctactttg aatgggtcca ggatgttcag cggttcttct ggaaagcgaa
     1801 agacattcaa gagcggcttc aagagataca gagttcggcg tttcaccgga ctctccagtt
     1861 tgctaccacc caaaagacct cgatgcgcat ggctgcgtta atgagcggca tcgacaaggt
     1921 cgcccaggca catctccaac gtggattgta cccatgacat cgacatctga ctttttttct
     1981 gtacggatcc gtcttgattg acacgcacta gccgtatctc gtgggagatt acgccctaac
     2041 cgcgattgcc ggcatctggt tcgcggatgg attattattg ctgctggttc ctcgattcat
     2101 catcacacac tttcggcaga ccctggccga gtcaccgaca attctgcgct gggaatggct
     2161 cgccgtcgct ggcgggggaa tcgtattgat tgcgggtcag catctccact atcaaccact
     2221 ttggataatt acggcgggag ttatgatcgt gaaagggctc tttttagcga tgggaccttc
     2281 cacatggcga acccgcgtcc tcgattggtg tctcagccgt gaagacgtgg attaccgctt
     2341 ctggggccta ggtctctgct ctcttgcgac attgctgctc catgcgttag ggtggatcag
     2401 cggctgatag cggtcagata tttggaacag attggatgac gcgtcatacc attgtatcgc
     2461 tctgggaagc ccacaaggtc gaacgctggc ctcgtctcga cagtcatcag gaagggccct
     2521 tgatgacgct ggacacggtg attagcggat gtgtggtgta ctatctcgat agcccagacg
     2581 ggcttgatgc ccaacgccgt acgattgtcg gggattgcct ggcagacctc gatatctttg
     2641 cggaagaact ggatgaagac tcccgacaat actttcagcg ccttcggcac ttaggctctc
     2701 tgctacttgc atcctccgac cccgactgat tctcggtaac gagaccagga gacgaactag
     2761 gccgcgccct cctccccgga ttcatggaac ctcaacaata tctttcttca tgggcgccaa
     2821 aagaccgagc aattcctcct tttctcttgg cggcacctta aatgtatcca gtgctttgat
     2881 caagtcttcg accaacgctc cgaaatccgc attcgagatt ttcatgccag catgcgtggt
     2941 tttcatgtca cggcccgcgt aggtgcaatg acctccggtc gccacgcaaa cttgatcgac
     3001 cagatgtcct ttcaacttcg gaatgtccgt cgtcgcaaac cgcccgttaa tccttgtatc
     3061 agctgccaca ttcgcgacaa actgatcgat gacggcagct atggccgtct tgcctcctag
     3121 ccgatcatac agagatttcc cggctggtat gggccctcgt tctgcacaac caaggagagc
     3181 aagaaatatc cccgctccga ccagccctgt cgatgttctc cggaacgacc tcattgaaac
     3241 acctccttgg taacgaacta tccgatcaga gattgaaaca tacatcgcgc acatgatcct
     3301 tgagttgcat gaacagatgc ctcatgacgc attacgttac gatcgccacg atctattcgt
     3361 ccaatatatt gttttcatca cattcatagg tataggctat tggtaagatt caacctgtat
     3421 ggaactacgt catcttcgtt atttcatcgc tgtcgcttca gcccagaact ttaccaaagc
     3481 tgctgcggca gtccatgtct cacaaccgtc attgtcagtg caggttcgtg cgctagaaga
     3541 cgagctgggt gctcgcctgt ttgaccggct cggccgcaag gtgacgctca ctcaagccgg
     3601 ggaattattc cgcgatcatg cggagcgcgc ggtccgcgaa cttgaacggg ctacgcaact
     3661 cgtccaggag gtgcatggag cgcaacgagg ccgcctcgtc gtcggtacgc tcgctactgt
     3721 gaattcctat ttgatccccc cactcgtttc gcaattcaaa caacggttcc ccggcattca
     3781 tcttcaaatc cattcgctac cctccgacga cattgtaagc agtatccttg ccaatcggct
     3841 tgatctaggc atttgtctgc ttccattggt acatggccgg ctctcgacag tcccattatt
     3901 cgaagagcgg ctagttctgg tcgcgcctag ggactaccct ctcagacaat cccgtcttcg
     3961 tatgaaagac ctcgcgggcc ttcccctcgt gctgatgccc gccgattact gcctccgaaa
     4021 gatggtggag gcagaatgta ctgaggcccg agtgaagacc gacgtggtgc tggagatgac
     4081 atcgccggag ggtattcttc aggcggtcgc tgaaggagct ggtctgacca ttttgcctga
     4141 attatacgtc agacttcgat ctcccggtgc gaggttccgt ctcatcgaac tctatgaccc
     4201 cgtgccgagg cattcagtcg gattggtttc ccttgcgaac cgataccaga atcttgcggc
     4261 gaaagagttt gcgacactct gtcgaaccac aatggcacgt ctgatgacca agtcacaatc
     4321 ctcctcatct cgccgacatc tgggacgtgc ataagtgtac tctcagtcca tgcggacagc
     4381 gctgagctgg ttatgctccc cattgcacac ccctttcgct ttcttgcaag ttgtgcaggc
     4441 cttcgttaga ataatcgtga ttggatggcc cgccacaatc gccatcaccc ctgaacactg
     4501 acccatccat cggaacagga aacgaggagg aatcaatgag gattatggtg cgagtcgtga
     4561 tgttgagcag cctgatggcg gctgttctcg tcggatgggt tataccgagt tatggagaac
     4621 cttacgagct gagtaagaac gatgtgatgg atcctaaaac ggtgaagagt gctgacatcg
     4681 ccctgtttgg agtgaagttg ggggatcctg aagccaaggc cctggacgtt ctcgttaacg
     4741 aaaagatccc tggcatcaaa gtcgagcagg aagctctttt tatcttcttg ctcgatcagc
     4801 gaaaaccgac aggcccgatg gccggcgtcc gtatccaaga tgggaaagtc gacctgatct
     4861 ttatcaataa ccgctttgcc tataagacgc gtggcatctt tcgaaacgtc ttgaacagcg
     4921 aaagtcctga agatgtgcgg aaactgttag gccaggaaga gtatggggat gaaaacgtga
     4981 tgggtgccgt gttggcctat gataagcagg gatttgtcgt taactatctt gggaaagacg
     5041 tcaacgtcga attttccacg cagcgttaag ctcaccgttt ctggaatctg atcgattcgg
     5101 cttcacagaa ccgggctttg cctgtcaacg cagcaaatac cctaaccata cgtcgtaatt
     5161 ttccttcgag aatctctcct gcaactccac cactccactt ccatcgcgag agagacaata
     5221 gctggatatt agtggacgat ggagttggcc tgaagtccaa agttgataag gttcttcgct
     5281 gtgtcccagc gacggttgga gttgaggatg accagcagga gatcgctccc attctgggaa
     5341 actttagcga tgaggcatcg tcctgctttg gaagtaaagc cggttttgac cccttcgacg
     5401 cccggtatgc ggccgaggag ccggttcgtg ttgtggagta cataggggcg atagccgttg
     5461 accggcacga tgatcgagtg ttcttcccga accagttggc ggaaggttgg ctgttcaagc
     5521 gcagtcaggc tcaactgagc caaatcctct gcagtagaat aatggtctac cccgtcaaat
     5581 ccgcaggcat tgctgaaatg cgtatctgaa agacccaggg cagcggcctt ggcgttcatc
     5641 agcgtgacga attggtcttc atcaccacca acatgctcga cagcagccag acaggcgtca
     5701 ttcgccgaca caatcaacat cgctttgagg agatcctcca ggcgaaaaac ctgccccggc
     5761 ttcaaccgca gatgggtctt gtgcgcccga gccgcattct tactgaccgt cacctgatcg
     5821 tcgagccgtc cctgttcaag aataacgagg gccgacatga tcttggttaa gctggcaggt
     5881 gacaaacgct ttcccgattc atgttcatag agcacgcgcc cgctattcaa ttccttgagg
     5941 agaatgctat gggcaggaac ttttcgccat gggagagggc gtccatctgc caatatggta
     6001 tgagcctggg gggcagaaat ccgatccttt tgtgccaatg ccgccgaggg tatgaagatg
     6061 gtgaggttcc acaacacaag caggattaag gcacacatga aggatcgcaa caaactggac
     6121 atatttgatg gcatcggaga gtccatggct gaatgttcag agcgtgaacg attcccgtac
     6181 ctttgagcct cggaagccgc tgtcgatgac tttgtgaaag aagcgaagca gatcagcaag
     6241 atcaatccaa aatccacaat tcaagcaccg tgcataaagt cggcgattca tcagcgtgta
     6301 atgacgctcg accatcataa accctttgca cttgaggcaa tccatccgtc accactcgag
     6361 aatgactggt tatcgaatcc gattcatgac caaggcaaca cagccggcca gaagtcctcc
     6421 accgaggatc gtcgtaccaa acgacagata tccgctctgg gaatgcgtcc cgtcaaaaag
     6481 gtctcggacg ccgccctgta ccatcagcac agagagagtg agcagcgctc cgatcgtaaa
     6541 ccaccacgca aaggatctca cgagtatcca caccgcagac tatctggggg ccttgaaccc
     6601 gctcctgacg gccactctag ccgagggtct gagagctgtc aagcagacag cggattcgtt
     6661 cgtccatcag tctgtttgag tatttcgcat gctcgtcatc gagcatcaga agccgaaaca
     6721 cgtccggact tcaagcctcg atgcaagaaa atggaaacgg atccgtttcc attatcagct
     6781 gtcacgaggc ccggttcttc ggcttccttt gtcgtcaccc gaaagaccgc cacagcaaaa
     6841 gggccggcct ttgtgcgata gtttttcggg ggatattcaa atgtcccgtc ccctcggcca
     6901 aataagatgg acaaatcgct cgactgcagg ttgacgatcg cgacatccgc atggcgatcg
     6961 ccgttgaaat ctcgggcgag accgaagttg ggcccggcat cggcaccgga atcacgaccg
     7021 gcctgaaatg tggcatctcc cttacccagg aaagtcgtaa aactgtctcg ttcaccgttg
     7081 atgacaagca tgtcttggac tcgatcgtcg ttgaagtccg cgaagcttat acccagtgga
     7141 cgtttcccgc tccgataatc cgtcgggcct cgaaatgtt
//
LOCUS       DATDNW010000289         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_659001, whole genome shotgun sequence.
ACCESSION   DATDNW010000289 DATDNW010000000
VERSION     DATDNW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659001"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VI359_06950"
     CDS             complement(<1..239)
                     /locus_tag="VI359_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY6974024.1"
                     /translation="MVANPGFPLSLDVRGWPVLVVGGDEEAAEKTQRLLEAGAKVTVI
                     SPTLHETLKQLAASAKVIHRGRHFRESDPENAILVL"
     gene            388..840
                     /locus_tag="VI359_06955"
     CDS             388..840
                     /locus_tag="VI359_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974025.1"
                     /translation="MAGYNVRTLGMAGSIIGLAAIAAWLGLAHSCDPQTGEPVIAGRV
                     VITQALADQIRPTDVLFVIVRRPGGMPRPVAAKRIENPKFPVSFEITNADVMVQGSEL
                     RGMVDVIARLDRDGQAGPAQPGDIEGRYAKNPTLPGGRDLEIVLDKMY"
     gene            complement(877..1422)
                     /locus_tag="VI359_06960"
     CDS             complement(877..1422)
                     /locus_tag="VI359_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY6974026.1"
                     /translation="MEMTLLLNSTYEPLRVVHWQKAISLLWQGKVEVLEVYDREIHGI
                     SISIKLPAVMRLLKLVKLKDSHRAVKFSRINIFTRDGYVCQYCKHKYRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNKKSGRTPEEAGMRLVKKPVKPRWSPVITITIGIRNT
                     PESWRDYLYWNMELDTDPADT"
     gene            1598..1918
                     /locus_tag="VI359_06965"
     CDS             1598..1918
                     /locus_tag="VI359_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6974027.1"
                     /translation="MSEFVTVAKVSEIPPGTGRTVEVHGVWIALFNVDGTFYAVDNTC
                     PHSGGPLGEGHLDGDIVECPWHGWQFNVRTGVRPEVPHFTVACCPVRIEADEVQVALP
                     STLM"
     gene            complement(1905..2351)
                     /locus_tag="VI359_06970"
     CDS             complement(1905..2351)
                     /locus_tag="VI359_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974028.1"
                     /translation="MVKILHYVLVAVLILGGLYYLWVTPPSLNPMADKRAAEALALVQ
                     THRALGYPSILQAMTEHVRSMKERGRGVRLGEWRVKQTDGDLYEIRVQLRDEGEKGQW
                     FEREFIWHAHLASKKVNAASLPADGVTPKAPELESPAPVPSNPTSG"
BASE COUNT          553 a          793 c          754 g          575 t
ORIGIN      
        1 agcaccagaa tggcgttttc cggatcgctc tcgcggaaat gccggccccg gtgaatgact
       61 ttggccgacg cagctaactg tttcagggtt tcgtgcaggg tcgggctgat cacggtcact
      121 ttcgcaccag cctcaagcaa acgttgtgtt ttctctgccg cctcctcgtc gccaccgacg
      181 accagcacag gccaccctcg gacatcgagt gacagcggaa atcctggatt cgccaccatg
      241 cttcttcctc ctcgagcaat agaaggtgct atcttacaac agcctcttcc gcagcgtaaa
      301 ccgtgccctc cccttttctt catcgacccg ttataatgcg ccgcgttatt acggtggcga
      361 tcagccctct gaaaggatcc ccctgccatg gcaggataca acgtgcgaac gctcggcatg
      421 gccgggagca ttatcggctt ggctgcaatc gccgcctggc ttggcctcgc ccatagttgc
      481 gaccctcaaa cgggcgagcc tgtcattgcc ggtcgggtcg tgattaccca ggcgctagcc
      541 gaccagatcc gccccacgga tgtgctcttc gtcattgttc ggagacccgg agggatgccg
      601 agaccggtcg cggcaaaacg gatcgagaac ccgaagtttc cggtctcgtt tgaaattacc
      661 aatgccgatg tgatggtgca ggggtcggag ctgcgcggaa tggtcgatgt gatcgccaga
      721 ctggatcgag atggccaggc cggcccggcc cagccgggcg atatcgaagg ccgctatgcg
      781 aagaacccca cgcttcctgg cgggcgagac ctcgagatcg tgctggacaa aatgtactga
      841 agcgagaact tgcaggaggg ctgtttcaac agcctgttag gtgtcggcag gatcggtgtc
      901 caactccata ttccagtaca aataatcgcg ccaactttcc ggcgtattcc tgatgccgat
      961 ggtgatggtg atcaccggac tccagcgagg cttaacgggc ttcttcacca gccgcatgcc
     1021 ggcttcttcc ggcgttcgac cgctcttctt gttattgcag cgccagcagg ccgtgacgat
     1081 attctcccac gtcttccgcc ctcccttggc gatgggaacg acgtgatcga acgtcagttc
     1141 ctccgtccga tacttgtgct tacaatactg gcagacgtac ccgtcccgcg tgaagatatt
     1201 gatgcgggaa aacttgacgg cgcgatggct gtcctttagc ttcaccagct tgagcagccg
     1261 catgacggcg ggaagcttga tggaaatcga gatgccgtgg atctcccggt cgtaaacctc
     1321 gaggacttca accttgcctt gccagaggag agagatcgct ttttgccaat ggacgacacg
     1381 cagcggttcg taggtggaat tgagcaggag ggtcatttcc attcaacaac ctcattccca
     1441 accgaggctc cttctatcag aaggcctggt atcggtctgg gtacgctcat gtatgtctat
     1501 gccataagac ctctgcgaaa tcaagcttcg ggccatgtgg cagcaccgga ccctgcgttt
     1561 gcctcaccaa cagtgccgat aaatcggggc gccttgtatg tctgaattcg taaccgtggc
     1621 aaaggtcagc gaaatccctc ccggcaccgg gcgcaccgtt gaggtgcacg gtgtgtggat
     1681 cgcgctcttt aatgtggatg ggactttcta cgccgtcgac aacacctgtc cacactcagg
     1741 aggaccattg ggcgaagggc atctggacgg ggacatcgtg gaatgcccct ggcatgggtg
     1801 gcaattcaac gtgcgaaccg gagtacgacc ggaggtgcct cactttaccg tcgcttgttg
     1861 tcccgtccgc attgaagcag acgaagtcca agtcgcgctg ccctctaccc tgatgtagga
     1921 tttgatggaa ctggcgccgg agattcaagc tccggtgctt ttggtgtcac cccgtccgcc
     1981 ggaagcgacg cagcgttgac cttcttggat gccagatgag catgccagat aaattcccgc
     2041 tcaaaccact gacccttttc tccctcgtcc cgtaactgga cccggatctc atagaggtcg
     2101 ccgtcagtct gcttcacgcg ccattctccg agtcgaacgc cgcgtccccg ttccttcatc
     2161 gaacgcacat gctcggtcat ggcctgcagg atactgggat atcccaacgc acgatgagtt
     2221 tggacaagcg cgagcgcttc cgcggctcgc ttgtccgcca tcggattgag cgaggggggc
     2281 gtgacccaca agtaatacag tcccccaaga atcaggacag ccaccagcac atagtggaga
     2341 atttttacca tcggaaggaa atgatctgag cgttatcggt cgcgcggcgg ttgcaacatc
     2401 cttcgcatga ccacggcaac aggcggaaga atttccgtct tacatacgca ccgtttccat
     2461 cttagggagg ctggaagacg gaagtcaacg catggctcat cgatgcgccg ggagccggca
     2521 gagcggcttg tgcgtgcgaa agtggttatg ttacatatac cgggcacaga ggagagccgc
     2581 gatgaagttt tttctgtacg ccgacaacct gaacccgact cagctcaagc ggcgcgcgcc
     2641 ggaacatcaa ttccagacga tcgccacgct gcccg
//
LOCUS       DATDNW010000290         3176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_926678, whole genome shotgun sequence.
ACCESSION   DATDNW010000290 DATDNW010000000
VERSION     DATDNW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926678"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VI359_06975"
     CDS             complement(<1..1152)
                     /locus_tag="VI359_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6974029.1"
                     /translation="MNRADRRRHEAQQARHGRPPDTVAIHALLDEGKAHHQAGRLPEA
                     ERAYRVALELTPQHPEALHLLGLLLYRLGRFDEAIDLITAAIEQQPDSPLYWFNLGVV
                     AQKACRREDAVRAYRKAITLNPRYLDAFINLGNVLKDQGAASEAKEVYQKALALNPSH
                     ADTHNNLGVTFKEEGRLEQAIDSYRRALQLKPTHIEALNNLGLALMEGGTLREAISSF
                     EQALTIMPGYVKALYNLGVARSRAGDDSSAVACLQKAADLKHNHGRTVTESTVYRSRI
                     SHDCEQIQYLLDRAILGEEHRPYLQELQRLRKELDRHPASGNRVPISPDTLAPVAPSF
                     NRLLYRAPAERLPQGAINPTLDVAAVESRYLSKQPEVTFIDGLLAQEALD"
     gene            1289..2224
                     /locus_tag="VI359_06980"
     CDS             1289..2224
                     /locus_tag="VI359_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEY6974030.1"
                     /translation="MKYALYSGCAAKGATPELYQSTMAIIGRLGIEVVELAAASCCGA
                     GVVTEANPDVALALNARTFAQAEALKLDVMTICGTCQGVMSAANRRFKTESGLLDRIN
                     QLLAPEGISYRGTISVKHLLWIVVREIGLARLRGLVSVPMREFRIAPFYGCYILRPSW
                     DLGFDDPENPQSLEKVITAVGGEPVAYTGRTQCCGFPIILEKEAIAVTMAGNHMKEAK
                     DGGADFMVTPCPLCHMSLDIYQERAGRAVNTSLNLPILHLPQLLGLAMGVPSIELGVS
                     RHLVPVDSIIRMIEKRQREKSEEPPSVGAQINSPS"
     gene            2267..2584
                     /locus_tag="VI359_06985"
     CDS             2267..2584
                     /locus_tag="VI359_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY6974031.1"
                     /translation="MRVINIAEYQQFSHEKMKKNNIFQTARLFCDIYCFEPGQEQKGH
                     VHGNQDKVYLVLEGQGTFQVGSEKQVLGPGQGTMAPAGQEHGVKNHTSQRLKVLVFVA
                     PNP"
     gene            complement(2739..3086)
                     /locus_tag="VI359_06990"
     CDS             complement(2739..3086)
                     /locus_tag="VI359_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974032.1"
                     /translation="MINRYFLLCLTLASGIICLPALAHPADERIALMLAGTACPQSHA
                     VLEQYLLRVPGVWQIDLHAIPDHILIDADMTVVAAGVLATQVNEVLAAQPPCRATIMK
                     SCISAELRMKGGR"
BASE COUNT          680 a          811 c          986 g          699 t
ORIGIN      
        1 gtcgagcgct tcttgggcga ggagtccgtc aatgaaggtg acttccggtt gcttggagag
       61 atagcgggat tcaaccgctg ccacatcgag cgtcggattg atcgcgccct gcggaaggcg
      121 ttcggctggc gcacgataca ggagccggtt gaacgacggg gccacggggg cgagggtgtc
      181 gggagagatc ggcactcgat tcccggaagc tggatgacga tcgagctctt tccggagtcg
      241 ttggagttcc tggagatagg ggcgatgctc ttctccgagg atcgctcgat ctagcaagta
      301 ctgaatctgc tcacagtcgt gggagattcg cgagcggtac acagtcgact ccgtgacggt
      361 acggccgtga ttgtgtttga ggtcggctgc tttctggaga catgcgacag ccgaagagtc
      421 atcacctgcc cgggaccgcg ccacaccgag attgtaaagt gccttcacgt accccggcat
      481 aatggtgagg gcttgttcga acgacgagat ggcctcccgc aacgtcccgc cttccatcaa
      541 ggcaaggccg agattgttca atgcttcgat gtgcgtcggc ttcagttgaa gcgcgcggcg
      601 gtacgagtcg atcgcttgct ccagccgacc ctcttctttg aacgtgacgc cgaggttgtt
      661 gtgcgtatcg gcgtgcgacg gattgagggc gagggccttc tggtagacct ctttcgcttc
      721 cgaagcggcg ccctggtctt tcagcacgtt cccgagattg atgaaggcgt caagatagcg
      781 tgggttcaac gtaatcgcct ttcgataggc acgaaccgca tcctctctac gacaggcttt
      841 ttgggcgacg acgcccaggt tgaaccaata gagcggacta tccggctgct gttcgatggc
      901 ggcagtgatg aggtcaatcg cctcatcgaa tcgaccgagc cggtacaaca agagtcccag
      961 gagatggaga gcctcgggat gttgaggtgt cagctcgagc gcgacacgat aggcccgttc
     1021 cgcttcaggg agccggccag cctgatggtg ggccttaccc tcatcgagca gtgcatggat
     1081 tgcgaccgtg tccggaggcc gtccgtgcct cgcctgttgt gcttcgtgac gtcgacggtc
     1141 cgcgcgattc attatcgtta ctctatggat acctggcggt tcgttcagac tagctgaaag
     1201 actggctcag cgtcaaagcc acctgatttt ggtcacgctt ctagcatata ctgccgtata
     1261 ggatgaaccg attggaggaa ttctcggcat gaagtacgcg ttatactccg gttgtgcagc
     1321 gaagggggcg acgcccgagc tgtaccaatc gacgatggcc atcattggtc gattggggat
     1381 cgaggttgtc gaacttgcag cggcgtcctg ttgcggggcc ggtgtggtga cggaagcgaa
     1441 tcccgatgtg gccttggccc tcaacgcaag gacattcgcc caagcggagg ccttgaaact
     1501 ggatgtgatg acgatctgtg ggacgtgtca gggggtcatg agcgcggcca atcgacgctt
     1561 caaaacggag tctgggttac tggatcgcat caatcaattg ctggcgccgg aagggatttc
     1621 ataccgtggg acgatttccg tgaagcacct gctgtggatc gtggttcgag aaattggact
     1681 ggctcgcttg cgcggactcg tttccgtccc catgcgagag ttccgcatcg ctccgtttta
     1741 cggatgttac atcttgcgtc cgtcatggga ccttggattc gacgatccgg aaaatcccca
     1801 gtcgctggag aaggtgatca cagccgttgg tggtgaaccg gtcgcctaca caggccgaac
     1861 gcaatgttgt ggatttccga tcattctcga aaaggaagcg atcgccgtga cgatggcagg
     1921 caaccacatg aaagaggcga aagacggagg ggctgatttc atggtgacac cctgcccctt
     1981 gtgccatatg agcctcgata tctatcagga acgggcgggt cgggcagtga atacgtcgct
     2041 caatctgccg atcctgcacc tgccgcagtt gcttggactc gccatgggag ttccgtcgat
     2101 agagttgggc gtctctcgtc atcttgtgcc ggtcgattct attatcagga tgattgagaa
     2161 acgacagcgt gaaaagagcg aagagccgcc atctgtcggc gcccaaatca actctccaag
     2221 ctgaaggtta gaggttcacg gcgatgtcgc ataggagata tgcacgatga gagtcattaa
     2281 catcgcagag tatcaacagt ttagccacga gaagatgaag aagaacaaca tctttcagac
     2341 cgcacgattg ttttgcgata tctactgctt tgagccgggc caggaacaga aggggcatgt
     2401 gcatggaaac caggataagg tctatctcgt gctagagggg caaggtactt ttcaggtcgg
     2461 atcggagaag caggtgttgg gaccggggca ggggacgatg gcccctgctg gccaggaaca
     2521 cggagtgaaa aatcacacga gtcagcggct caaggtgttg gtgtttgtgg ccccgaatcc
     2581 gtgacccgac ggcaatgcaa gcggggttgc taccaatggc tgttccggga aaatcttcct
     2641 ccgtatttcc ttcatttcac gtctgcgcgc cacaataaaa agtcgcggct gatccgggat
     2701 ccttgcatgt catcgctctc taatctgtta ttgctgcatc atcggccgcc tttcatgcgc
     2761 agttcggctg aaatacaaga tttcatgata gtggcgcggc aagggggttg tgcagcgagg
     2821 acctcattga cctgagttgc cagcacccca gcggcgacca cggtcatgtc agcatcgatg
     2881 agaatatggt cagggatggc atggagatca atctgccaaa cgcctgggac ccgtaagagg
     2941 tactgttcca acactgcgtg gctctgtggg caagcagttc ctgcgagcat cagggcaatc
     3001 cgctcatccg ctggatgggc gagagcggga aggcaaatga ttcccgaggc gagtgtgagg
     3061 cagagaagga agtatctgtt tatcacgacc ttaaaagaat catctgcccc ggctggccag
     3121 atcaaaggag ccaaacggtc cactatcctt tctagggagt tggaacgggc gtaatc
//
LOCUS       DATDNW010000291        12737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1137036, whole genome shotgun sequence.
ACCESSION   DATDNW010000291 DATDNW010000000
VERSION     DATDNW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137036"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VI359_06995"
     CDS             <1..648
                     /locus_tag="VI359_06995"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY6974033.1"
                     /translation="RENSFGVNIMLPFEQGPNATIADDPKLVTFKYFFTRKLMFQKEA
                     NAIALFPGGFGTHDEGFEILTLAQTGKSDPMPIVCLQASGCDYWDQWAAFINAQLLAR
                     KLINEEDLNLFKIVTSADEAVAEIMAFYRRFHSIRYVGRQLAMRLTQAPSPEQVANIH
                     TRFADLLSEGTFELRGPLPEELDEPELIDLPRLVFTSNRRSAGRLRQLIDHLNTL"
     gene            706..1584
                     /locus_tag="VI359_07000"
     CDS             706..1584
                     /locus_tag="VI359_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEY6974034.1"
                     /translation="MAGPHQPHRFSKPPTAILYHAECADGFGAAWAIWKQFPGAQFIP
                     VKHGNPPPPELTTQRVVIVDFSYDRPTLEHMASETQALLVLDHHITAEKALAGLPYAY
                     FDMKKSGAVLAWEWAHDHPVPWLLDYIQDKDLWTWALPSSREINAAVASHPFDFDLWN
                     GFKQKELEQEGRAILRYEHELVNKLAAQAVLVEFQGAVVPSVQSAVLTSQIGERLSAD
                     YPFCIIWHDREGRRYYSMRSREDGSDVGAIAASFGGGGHTHAAGFSIPLGPDGSLPDN
                     PALPRSIVDRRRPSAS"
     gene            1592..2287
                     /locus_tag="VI359_07005"
     CDS             1592..2287
                     /locus_tag="VI359_07005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY6974035.1"
                     /translation="MIPLHDDNPTEHTPVVTITFIVTCVLVFLYQASLADKFGELFVF
                     QYGAIPAVVFGQADLPEDAGRIPAYATLLTSMFLHGSWMHLFGNMLYLWVFGNNIEDV
                     MGHAKYIVFYVTCGILAALSHAMTDPSSTIPMVGASGAISGVLGAYVLLFPRAHVLVL
                     MPGLGMTQVAAAVVLGMWFVLQLLSGGMSLASSGGGVAFFAHIGGFVAGMALIGFFKR
                     PDVPFFAPSRTNR"
     gene            complement(2327..4177)
                     /locus_tag="VI359_07010"
     CDS             complement(2327..4177)
                     /locus_tag="VI359_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6974036.1"
                     /translation="MSAATLLESPPIDDTAVTASEAFPVSRTHYLVLQTLVGIVLGYQ
                     LWFGVEPIAIRSLSGLILFGLAVMLMTLLLVPKTILQATWFNAGLVGLDTAFVTATIY
                     LSGNARSDLYLSYFILMLIAASLRRLTHIIGFSLLLCAGYGFILYGGIIQTGTLSVGH
                     LLGAPVMLVMAVLYGMALESIGIERQQKTLLLESIGSLRKTERILQASRDDLQARVHG
                     LKHDLSRANEHVQQEKTEREGLERQLQDVQKMEALGRIAGNIANEFSHLLSVIGKQTG
                     VVLSRLKPDDPLYRPVDEIFRTGEQASALTAQLATLGIQEGQVRQVLSLKTVLAEVHS
                     VIKGLLSDRIDLQIAVEDRPLVVEVDREGLEQVLFQLTVNARDAMPKGGRLQIEVKQV
                     SGSSDARQPPVLQNQKPWVLIQVRDTGSGMNRDTQSRMFEPFFSTKETNVGLGLTAVY
                     GIVKQSAGHVEVASQPGQGTIVRVFLPLAVQARHTALPLSKAITAKANETVLLVEPNE
                     IGRKLALSTLLRHRYHVLEAGSPVEALLLTQQHPGPVHLTVSDLVMPEISGRDLARRL
                     IKQYPTMKALFVSGYDDEAILSHRINRQYLLRRPYRQAGLVEKVRELLDA"
     gene            complement(4452..5621)
                     /locus_tag="VI359_07015"
     CDS             complement(4452..5621)
                     /locus_tag="VI359_07015"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY6974037.1"
                     /translation="MTFTVIEPGSVLGVLGGGQLGAMFTMAAQRMGFQVAVWDPDADA
                     PAHRLAQRSFSTSFSDQRALGQFLDLVSAVTYEWENVPVSLCGTLEATKPVRPSSAVL
                     HTIQDRLEQKGFLAARGFPVVPFLPLTKPEQLPAVVGKIGYPALVKTATTGYDGKGQW
                     RITCKTDAEQVERTLYDSARPGIRWIVEGLVTFDRELSILIVRGMDGQTGVYPVAENE
                     HEAGILRTTLVPARVPSEIAARAAVLARQVVEALQGVGVFCLELFYLSTGELLINEIA
                     PRPHNSGHYTLDACSVSQFEQQVRALCGLPLGEIRLLKAAAMVNLLGNDATTISEPSC
                     EALLDTPGTFLHLYGKRTMRSRRKMGHVTFVADSLNLAQERAQQLRRRLASGLRT"
     gene            complement(5618..6196)
                     /gene="purE"
                     /locus_tag="VI359_07020"
     CDS             complement(5618..6196)
                     /gene="purE"
                     /locus_tag="VI359_07020"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY6974038.1"
                     /translation="MAHVVHGSRSRQQGQKRSRPLVGVLGGSKSDFPILEKAVAVLTE
                     LGVPNELMVVSAHRTPDRLFVYAEQASARGIEVIIAGAGGAAHLPGMLAAKTHLPVIG
                     IPIPTENLRGLDSLLSIVQMPRGIPVATVAIGGAENAGLLAAQILAGRYPEIEARVKA
                     FRKRQTEGVLHSPEAKGMVAGGTTPRHPSRRS"
     gene            6179..6322
                     /locus_tag="VI359_07025"
     CDS             6179..6322
                     /locus_tag="VI359_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974039.1"
                     /translation="MDDMGHKTKSPGSFDIYDYRRDFTIAVFLLTVKIMLALSNSAFL
                     LKN"
     gene            6398..7834
                     /locus_tag="VI359_07030"
     CDS             6398..7834
                     /locus_tag="VI359_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY6974040.1"
                     /translation="MARLTEGLRNKVRSLINQDTGLDQIAVDDLATSNKLQAWEAVEI
                     PERLRFSSRLAIKLVLLFILIIAFVPWTQTVTVTGQLSAYTPFERPQDIEAQITGRIK
                     KWHIFEGVRVKQGDLILEMDDYDPNFMSPDLLSFLEQRRKALTDTRKAALGRAEQLDK
                     RISEMQNLVKAAVPSAQARVVEAENKVREAHQKVEAAKIAVATAELNVDRHKQLAQEG
                     LVSQRELELTIQAAIATKAELTGAQANLKAAEQGMKALSFGRDQVSAEVLQRLLDAEA
                     ARDASVADAAKAADQLADVSLRMSNAEQRRLASRIFAPIDGTVVKMAQAGAGETVRQG
                     DKIVRLSPASTDKAIEMMADGLDAPLLNVGRKVRILFYGIPAIPLPAWPEIMAGTFGG
                     VIKVIDQVDDGKGNFRFWVVPDPDDRPWPPQEHIRQGTKAMGWVILNRVPLWYELWRR
                     FNLFPPDYQERPPSLIDTLLPKAGRGAK"
     gene            7957..9444
                     /locus_tag="VI359_07035"
     CDS             7957..9444
                     /locus_tag="VI359_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6974041.1"
                     /translation="MNLYQRCLVVVLAGTLGLCPLSAPAGESMKSKDLPPIPLSLDEI
                     QAWIDRSHPLLKGAGTEKVMARGKMLKALGAFEPTVVNDTELERFISSSNPNAGTQTV
                     GFNDTLVEARHPWGFKYSAGVREVINGPAKIPDMSFNNGNSQVLLGGSFPLLKGLLTN
                     PENAELQRSELADPRADVRVAQTRQDLFLAAAIQFWDWVASVKLAEVQRKALGVAEER
                     HRQVEGRAKAGAVAPLDVVEANQEVQRRREVAIAAQRGVEQEQLKLSMFLWERNVPVT
                     PPVERAPEFPSQMLIPSPDAIQAHKLQAKADRPEIKEIDIEAKLNNIDLALAKNNLLP
                     SLDAEAAPARAPEKFVLGLGYRFGVELRIPILQRKSRGEVLEAQGKADRFVLVQKFRE
                     QQVVIDVDNALSAIERAKERVAAATDSLRLAKTLEEGERFRFSLGATSVLFVNLRERN
                     SVDSENQVIRAKADYQKAQALYQWAIGAWARSLPSAIPVSYRARD"
     gene            9591..11252
                     /locus_tag="VI359_07040"
     CDS             9591..11252
                     /locus_tag="VI359_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6974042.1"
                     /translation="MERHAGNQPNLFKSVLGYLSVLFRLERRLLALVVSYSLAIGLFS
                     LIVPLTVQELVNTFAFAIQPITIVTLAGVMVAALLFVGAFRTLQYYAVEVLERRFFAR
                     VAIGMAKHLPHMQFLGFKPRYANYFMETVFMQRALSVLLVDLINVIVGGAVGMTILVF
                     YHPYFLLYNALLLAGFNVVFLLSQGGLKATIDMSHAKYDTLHWMQEISYNLLHFKATD
                     SQTLLMQKTDELVNKYVETRRTRFGILVRQYLGSVGWQAFAHSGLIATAGWLLSIGQL
                     TLGQLVAAEVVVSGLLASFDEVVKRMGHIYYFFTGLTELDFLFSLPKDQKSDRLSVPL
                     PDPTIHGIRVTCKDLTMEHPGMPPIFQSFDLEVTPGEKVGIYASTTAAKTALARVLAG
                     LEDPTSGVIRYNGVDLRHLDLNSINRCRGFMIDSQLSLFEGTIEDNIVLGRSYIPYND
                     VRWALRVTELEEDVDALPHGLKTHIRAPGKILAPTHIMRILLSRAILARPQMLIFDGI
                     LHNLQPAMRETILRRLCSKDEPWSVIFVSNDPNLTPHVDRRLILE"
     gene            11266..12060
                     /locus_tag="VI359_07045"
     CDS             11266..12060
                     /locus_tag="VI359_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEY6974043.1"
                     /translation="MGLSSYLRSYRPSGAQIIISLLIAGLGWLSGQALSQVDQDMRIM
                     YTEYTLGAADLAHISADVIRYRNTIIRALEADNKKDFERITESLPIQRAKIQHAVDRY
                     AAAGLRVSRSGRSEPEDIEAVRRSLDQYFSVASRTIDLLTQVWNAPLQPDREALRRKA
                     EEHAADNGGPKMIQVSLALDRLLDTVADVAKDMRDEGTKTIQKTSVLVIGGSFFIAIL
                     NLFLAAPRRRDRNRDDTEVHRMIEAPDAHVSLPLGNDRPNPVLRTE"
     gene            complement(12090..>12737)
                     /locus_tag="VI359_07050"
     CDS             complement(12090..>12737)
                     /locus_tag="VI359_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY6974044.1"
                     /translation="LIEGGRVTINGRPAKLGDQIAVGDVIARDGQVIPWGNKAIYIKY
                     HKPIGVTTTSESHVTRNIIAAIGHPERIFPIGRLDKDSSGLILLTNDGDIVNEILRTE
                     FGHEREYHVSVDRPFDQAFLDRMVQGVVILGRRTKPCWVERLGPARFRIILTEGRNRQ
                     IRRMCHALGYRVLTLHRVRIMHITIAGLAPNTWKDLTDEERTKLLQEVERGAVSK"
BASE COUNT         2786 a         3691 c         3506 g         2754 t
ORIGIN      
        1 cgcgagaaca gcttcggcgt caacattatg ctgcccttcg aacaggggcc taatgcgacg
       61 atcgccgacg atcccaagct ggtcacgttt aaatacttct ttacgcgcaa actcatgttt
      121 caaaaggaag ccaatgccat cgccttattc cctggcggat ttggcaccca tgatgaggga
      181 tttgagattt tgaccttggc acaaacgggc aagagcgatc ccatgccgat cgtgtgcctt
      241 caggcgtccg gatgcgatta ttgggaccaa tgggcagcct tcatcaatgc tcaactgctc
      301 gcccggaaac tgatcaacga agaggatttg aacctgttta agatcgtcac atctgcggat
      361 gaggccgtgg cggaaatcat ggccttctac cgacgcttcc actcgattcg ctatgtggga
      421 cgacaattgg ccatgcgcct cacgcaagct ccctcgcccg agcaggtcgc gaacattcac
      481 acgcggttcg cggatctcct gtctgaagga accttcgaac tccgcgggcc ccttcctgaa
      541 gaactggacg agccggagct catcgacctc cctcgcttgg tgttcacttc aaaccgccgg
      601 agtgccggcc ggctacggca gttgatcgac cacctcaaca ccctttaatc gaattcgcca
      661 agagtcttta gtcctgtcgg agtgccgtgg tatgctccca agactatggc cggtccgcac
      721 caacctcatc gtttctccaa acctccgact gccattcttt atcacgccga atgcgcggat
      781 ggctttgggg ccgcatgggc gatctggaaa caatttccgg gcgcgcagtt tattccggtg
      841 aaacacggca atccgcctcc tcccgaattg accacacagc gcgtcgtgat tgtcgacttc
      901 agttacgacc gacccacgct ggaacacatg gcctccgaga cacaggccct gttggttctc
      961 gatcaccaca tcactgcaga gaaagcgttg gccggtctcc cctatgccta tttcgacatg
     1021 aagaagtccg gggcggtgct ggcgtgggaa tgggcccatg atcatcctgt accgtggctg
     1081 ctcgactata ttcaggacaa agacttgtgg acgtgggcgc ttccgtcgag tcgagaaatc
     1141 aacgccgccg tcgcgtccca cccgtttgat ttcgacctct ggaacggatt caagcagaag
     1201 gagctggagc aggaaggccg cgccatcctg cggtacgagc acgagttggt gaacaaactt
     1261 gccgcccaag ccgtcttggt cgagtttcag ggggctgtcg tcccttccgt gcaaagcgcg
     1321 gtcttaacca gtcagatcgg cgaacgactc tccgccgatt acccgttctg catcatctgg
     1381 cacgaccgcg aggggcgacg gtactacagc atgcggtctc gagaggatgg atctgatgtc
     1441 ggcgccattg cggcctcgtt cggcgggggt ggccatacac acgccgctgg tttttctatc
     1501 cctctgggcc cggatgggtc cctacccgac aatccggctc ttccccgctc gatcgtcgac
     1561 cgtcgacgtc cttccgcctc gtgagggctc catgattccg ctccacgacg acaatccgac
     1621 agagcacaca cccgtcgtca cgatcacgtt catcgttaca tgtgtcctcg tgtttctgta
     1681 tcaagccagc ttggccgaca agttcggaga attgtttgtc tttcaatacg gcgctattcc
     1741 cgctgttgta tttggtcaag ccgacctccc agaagacgcc ggtaggattc cggcctatgc
     1801 cacactgttg accagtatgt tcttgcacgg cagttggatg cacttgttcg gcaacatgct
     1861 ctatctctgg gtatttggaa acaatattga agatgtcatg ggtcatgcca agtacatcgt
     1921 cttctacgtt acgtgcggga tcctcgcggc tttaagtcac gcgatgaccg atccctcgtc
     1981 gactattccg atggtgggag ccagcggagc gatttcgggc gtgctggggg cgtacgtgct
     2041 gttatttccc cgcgcccacg tgctcgtgct gatgccggga ctgggcatga cgcaggtcgc
     2101 ggccgcagtt gtgctcggga tgtggtttgt gctgcaacta ctcagcgggg ggatgagctt
     2161 ggcaagttcg ggtggcggag tcgccttctt cgctcatatc ggaggttttg tcgccggaat
     2221 ggcgctcatc ggattcttca aacgaccgga tgttccgttc tttgccccga gtcgaacaaa
     2281 ccgatgagat atctagtacc gtcggtgcat ggcgagcaaa tccctctcaa gcgtccagaa
     2341 gctcccgaac cttttccaca agcccggcct gccgataggg gcgcctcagc aagtattgtc
     2401 gattaatacg atgcgagaga atcgcctcgt cgtcataccc agatacaaac aatgctttca
     2461 tcgtgggata ctgcttgatc aaccgccgcg cgagatcgcg cccgctaatc tccggcataa
     2521 ccagatcgct cacggtaaga tggaccgggc cgggatgttg ctgtgtcagc aagagcgctt
     2581 cgacagggga tccggcttcc aacacgtgat agcgatgtcg tagaagcgtc gagagggcga
     2641 gtttccggcc gatttcattc ggctcgacca gcaatacggt ttcgttggct ttcgccgtga
     2701 tagccttcga tagtggcagg gccgtgtggc gggcctgcac cgcgagcggc aagaagacgc
     2761 gaacgatagt gccttgtccc ggctggctgg ccacctcgac atgcccggcg ctctgtttca
     2821 cgatgccgta aaccgccgtt agcccaagcc caacgttggt ttctttcgtc gaaaagaacg
     2881 gctcaaacat gcgtgactgc gtgtcacgat tcatcccgct gccggtatca cgcacctgaa
     2941 tgagtaccca gggtttctga ttctggagaa cggggggctg ccttgcgtcc gagctccctg
     3001 aaacttgttt gacctcgatc tgcaggcgac cgcccttcgg catggcatcg cgcgcgttga
     3061 ctgtaagttg gaacagcact tgttccagtc cttcccgatc cacttccacc acgaggggtc
     3121 tgtcctctac cgcaatctgc agatcgatac gatcggacaa cagacccttg atgacgctgt
     3181 ggacctctgc aagcacggtc ttgagagaca gcacttgacg gacttggccc tcctgaatac
     3241 cgagcgtcgc caattgagcc gtcaacgccg aggcttgttc tccagtacga aaaatctcgt
     3301 cgacaggcct atagagcggg tcatccggtt tgaggcggga cagcaccacc cctgtttgct
     3361 ttccgatcac cgagaggaga tgactgaact cattcgcgat attgccggca atccgtccga
     3421 gcgcctccat tttctgcaca tcttgcaatt ggcgttcgag tccctctcgt tctgttttct
     3481 cttgttggac gtgctcattg gctcgcgaaa ggtcgtgttt aaggccgtgg acgcgggcct
     3541 ggagatcatc gcgagacgcc tgcaagatcc gttcggtttt tcgcagcgag ccaatgctct
     3601 cgagcaagag agtcttctgt tgccgttcga tcccgatcga ttccaaggcc ataccgtata
     3661 acacggccat caccagcatt accggggcac cgagcagatg gcccaccgat aatgtccctg
     3721 tctggattat gcctccgtat aagatgaaac catatcccgc acacaacaga agagaaaatc
     3781 cgatgatatg agtaaggcgg cggagcgagg ctgcaatcag catcaagata aaataggaaa
     3841 gatagaggtc ggatcgtgca ttcccagaca ggtaaatcgt cgcggtcacg aaggctgtat
     3901 ccagcccgac gagccccgcg ttgaaccagg tcgcttggag gatcgtcttt gggacgagca
     3961 gaagcgtcat gagcatgacc gcgagtccga acaagatcaa tccactgaga ctccggatgg
     4021 caatcggttc gacgccaaac cagagttgat agcccaagac aatgccgacc agcgtttgta
     4081 gaacgagata atgcgtgcgc gagacgggaa acgcctcgga ggcagtgact gcagtgtcat
     4141 cgattggtgg ggattcgaga agtgttgccg cgctcacaat ggtatacgcc cttgttctgg
     4201 agactgagag accagcaatt cttgtaccga tggtcgaatc aggcagggcc tcgtttttgt
     4261 tacaaacgtt ccgtgtgggg gagcggagtg gttggaacaa gtcctacagc aggtaacaaa
     4321 atcgaacgaa atcggagcgt caaaaattgg cccaggactg tttgctcgga gatgaacctt
     4381 cttcctttgt cctcgaacga gctggacaaa gcaatgaggt ggcccttcct aatgagagat
     4441 tcgcaccgag tttaagtcct cagaccacta gccaaccggc gacggagttg ctgcgcacgc
     4501 tcctgtgcga ggttcaacga atcggcaacg aacgtcacat ggcccatctt tcgtcgagat
     4561 cgcatggtcc gcttcccata aagatgaagg aacgttccag gcgtgtcgag cagggcttca
     4621 cagctcggtt cgcttatggt cgtcgcatcg tttccaagga gattcaccat ggcagcagcc
     4681 ttcaggagac gtatctcacc gagcggaaga ccacaaagtg cccgtacctg ttgctcaaat
     4741 tgagacacgc tgcaagcatc gagggtgtaa tgtcctgagt tgtggggtcg aggagcaatt
     4801 tcgttgatca gcaattcgcc agtagagaga taaaacagct caagacagaa cacgccgaca
     4861 ccctgaagcg cctccacaac ttgtcgggcg agcacagcag cacgggcggc aatctccgaa
     4921 ggaactcgcg ccgggactag agtcgtgcgt aggataccgg cttcatgttc gttctcggca
     4981 acgggataga ctccggtctg cccgtccatt ccgcggacga tgagaatcga gagttctcgg
     5041 tcaaacgtga cgagcccttc aacaatccat ctgatcccag gacgagccga gtcgtacaag
     5101 gttcgttcga cttgctcagc atcagtctta caggtgattc gccactgacc cttcccgtcg
     5161 tagcccgtcg tagccgtctt cacgagcgcg ggatacccga tcttccccac cacagccggc
     5221 aactgctccg gcttcgtcaa gggaagaaac ggcacgacag gaaatcctcg ggcggccaaa
     5281 aaccccttct gctcgaggcg gtcttgaata gtgtgaagaa cggcgctcga cggccgaacc
     5341 ggcttcgtcg cctccaacgt cccgcacaaa ctcaccggaa cgttttccca ctcataggtc
     5401 accgcgctga ccaggtcgag gaactgaccg agcgcgcgtt ggtcggaaaa ggaagtagaa
     5461 aaggatcgct gggccaaccg gtgggctggt gcatccgcat caggatccca gactgccacc
     5521 tggaaaccca ttcgttgggc agccattgtg aacatagcac ccaactgccc gccaccgagc
     5581 acaccaagaa cacttccggg ttcaatcacc gtgaatgtca cgaccggcga ctcggatgac
     5641 gaggcgtggt gcccccggcc accatacctt tcgcttcggg agaatggaga accccctcgg
     5701 tttgtcgttt tcggaaggcc tttacgcgcg cttcaatctc aggatatcgg ccggcaagaa
     5761 tttgagccgc gagcagcccc gcattctctg cacctccgat cgccactgtt gccacgggaa
     5821 ttccgcgtgg catctggacg atagaaagca acgagtcgag ccctcggaga ttttccgtcg
     5881 ggatcgggat gccaatgacg ggaaggtgtg tttttgcggc gagcattccg gggagatgag
     5941 ccgcaccacc cgctccggcg ataatcactt cgatacctcg tgccgaggcc tgctcggcat
     6001 agacaaacag tcggtccgga gtccgatgcg ccgagacaac catcagttca ttgggaactc
     6061 cgagttcggt gagtactgcg actgccttct caaggatagg aaagtccgac ttgcttcccc
     6121 ctagaactcc gaccagcggg cgggatcttt tttgtccttg ttgtcgagat cgactgccat
     6181 ggacgacatg ggccataaga caaaatcccc tggttccttc gatatctacg actatcgcag
     6241 ggacttcacc atagcggtat tcctattgac ggtcaagatc atgctagccc tgtcgaacag
     6301 cgcgttcttg ctcaagaatt gacccattga gccgctttcc tctatgatga agcagtcatt
     6361 gactcgctcg ctccttcctt catctgagga ggacgtggtg gcacgcctga ccgaaggcct
     6421 cagaaacaaa gtccggtctc tcatcaacca ggacaccggg ctcgaccaga ttgcggtcga
     6481 tgacctcgcc acttccaaca aactccaagc ctgggaagcg gtcgaaatcc ccgaacgcct
     6541 tcgcttttcc tctcggcttg ccatcaagtt ggttcttctt tttattttga tcatcgcctt
     6601 tgttccctgg acccagacgg ttacagtgac aggccagctt tctgcttata cccccttcga
     6661 acgcccgcaa gatatcgagg cgcagatcac cggacgaata aagaagtggc atattttcga
     6721 aggtgtgcgg gtcaagcaag gcgatctgat tctggagatg gacgattacg accccaactt
     6781 catgtccccc gatttgctct cgttccttga acagcggcgg aaggccctga ctgatactcg
     6841 gaaggccgct ttaggccgcg ccgagcaact cgacaaacgc atcagcgaga tgcagaacct
     6901 cgtaaaggcc gcggtgcctt cggcacaggc ccgcgttgtc gaggcagaaa acaaggtccg
     6961 tgaagcccac cagaaggtag aagcggcaaa aatcgcggtc gcgacggcag agttgaatgt
     7021 ggatcgtcac aaacagctgg ctcaagaagg attggtgtct cagcgtgagc tcgagttgac
     7081 gatccaagcc gccattgcga cgaaggcaga attgacggga gcccaggcca atctcaaagc
     7141 ggcggaacag ggtatgaaag cgttgagttt cggtcgcgac caggtcagcg ccgaggtgct
     7201 gcaacgtttg ctcgatgcgg aagccgcgcg tgacgcttcg gtggctgacg cagccaaggc
     7261 cgcagatcag ttggcagatg tgtcgttacg tatgtccaac gctgaacaac gccgtttggc
     7321 cagccgcatc tttgctccga tcgacgggac ggtcgtcaag atggcgcaag ccggcgctgg
     7381 agaaaccgtc cggcaaggag ataaaatcgt tcgcctttca ccagccagta cggacaaggc
     7441 tattgaaatg atggccgacg ggttggacgc gccgttgctc aacgtcgggc gcaaagtccg
     7501 cattctcttc tacgggattc ccgccattcc tcttcccgca tggcccgaga tcatggccgg
     7561 caccttcgga ggcgtaatca aagtcatcga ccaagtcgac gacggcaagg gaaacttccg
     7621 tttctgggta gtccctgacc ctgatgatcg cccgtggcct ccccaagaac acattcgcca
     7681 gggtacgaaa gccatgggat gggtcattct gaatcgcgtc cctctctggt acgagctgtg
     7741 gcgacggttc aatctctttc cgccggatta tcaagaaaga ccgccaagct taatcgatac
     7801 cctcttgcca aaggcgggac ggggtgcgaa gtaagcacga cccggcatct gctgtctcat
     7861 ctcaggtttc caacgtcacc cgacatacat cgcgtttgta ccggtcatag gctcggtcag
     7921 catcggctta tccgcatcat gaaaggagtg tgcgctatga atctctatca acggtgcctc
     7981 gtcgtcgtgc ttgcagggac acttggcctc tgccctctct cggcccctgc cggagagtcc
     8041 atgaagtcta aagaccttcc acctatcccg ctcagcctgg atgaaatcca ggcctggatc
     8101 gatcggtccc atcccctcct gaagggagcg ggaaccgaga aggtcatggc gcgcgggaaa
     8161 atgctcaagg cgctgggcgc gtttgagcca accgtggtca acgacacgga actcgagcga
     8221 tttatctcaa gctccaatcc aaacgcaggc acgcagaccg tcggcttcaa cgacacgctg
     8281 gtagaagccc gtcatccctg gggatttaag tacagcgccg gcgtccgtga agtaatcaac
     8341 ggtccggcca aaattcctga catgtccttc aacaacggaa acagccaagt gctcctcggc
     8401 ggatcctttc cactcttgaa aggcctccta acgaacccgg agaacgccga actgcagcgg
     8461 tccgaattgg ccgatccacg cgctgatgtg agggtcgccc agaccaggca ggacctgttt
     8521 ttggcggcgg cgatacaatt ctgggactgg gtcgccagtg tgaagcttgc ggaagtccaa
     8581 cgtaaggcgc tgggcgtcgc ggaggagcgc catcggcaag tcgaggggcg cgccaaagcc
     8641 ggcgccgtgg cccccctcga cgtcgtggaa gctaatcaag aagttcagcg acggcgagag
     8701 gtggcgatcg ccgcccaacg aggcgttgaa caagaacaac tcaagctgtc gatgttcctc
     8761 tgggagcgca acgttccggt gacgccgcca gtggaacgag cccctgagtt cccctcacag
     8821 atgttaatcc cttctccgga tgctattcag gcgcataagc tccaagcaaa ggccgatcgt
     8881 ccagaaatca aagagatcga tattgaagcc aagctcaata atatcgacct ggcgcttgcc
     8941 aagaacaatc tccttcccag tcttgatgcc gaagcggcac ctgcacgcgc cccggaaaaa
     9001 tttgtcctcg gtcttggcta tcgattcggg gtcgaactca gaatccctat cctgcaacga
     9061 aagagccgcg gcgaggtttt agaagcgcaa gggaaggctg atcggtttgt cctagtgcaa
     9121 aaattccgtg aacaacaggt cgtgatcgac gtcgacaacg ccctgtcggc catcgaacgg
     9181 gcgaaagaac gggtggctgc cgcaactgac tcattgcgac tagccaagac gcttgaggaa
     9241 ggagagcggt tccgtttcag cctgggcgcg accagtgtct tgtttgtcaa cctgcgagaa
     9301 cgcaactccg tcgactctga aaatcaagtc atccgagcca aagccgacta tcagaaagcc
     9361 caagctctgt accaatgggc cattggtgcc tgggcgagga gcctgccgtc ggccatcccg
     9421 gtaagctacc gtgcgcgtga ttgagatgga agaagtggct ttttctactg tattttgata
     9481 gcgttagaca cgtgccgtct tgtgagaggg acacagcttt tccctaaccg agaccgacga
     9541 caacagcgca gacctgcaga aggccggcgc aaacaagaaa gcgaaaatcc gtggagcgcc
     9601 acgcgggcaa ccagccgaat ctgtttaagt cggtgcttgg ctacctcagc gtgctctttc
     9661 gcttggaacg gcgtctcctc gccctcgtgg tgtcatattc gcttgccatc ggtctgttct
     9721 ccctcatcgt cccgttgacc gtccaggaac tcgtcaacac gttcgccttt gctattcagc
     9781 ccatcacgat cgtgaccctt gccggtgtca tggtggccgc ccttttattc gtcggagcct
     9841 ttcgcacgtt gcagtactac gccgtcgagg ttttggaacg gcgcttcttt gcccgtgtcg
     9901 cgatcggcat ggcgaaacac cttccgcaca tgcagttttt aggattcaag ccgcgctatg
     9961 ccaactactt tatggaaacc gtgttcatgc aacgggcgct ttcggtgctc ttggtcgatc
    10021 tcattaacgt gatcgtcggc ggcgccgttg ggatgaccat cctggtcttc tatcatcctt
    10081 attttctcct gtataacgcg ctgctcttgg ccggcttcaa cgtggtcttc ctgttgtcgc
    10141 agggaggact caaagccacc attgacatgt cccacgccaa gtacgacacg ctgcactgga
    10201 tgcaggaaat ctcctataac ctgcttcatt ttaaggcgac cgacagtcag accctcctga
    10261 tgcaaaagac cgacgaattg gtgaacaaat acgtcgagac ccgtcgaacg agattcggaa
    10321 tacttgtccg gcagtatttg ggatcggtcg gctggcaagc gttcgcccac agcggactca
    10381 tcgccaccgc cggatggctc ttatcgatcg gccaattgac ccttggacaa ttggtcgcgg
    10441 ccgaggtcgt cgtcagcggc ctcctcgcga gcttcgatga ggtcgtcaag cggatggggc
    10501 acatctatta tttcttcacc ggattgaccg aactggactt cctgttctcc cttcccaagg
    10561 atcaaaagtc ggataggctc tcggtgccgc ttccggaccc gacaatccac ggtattcgtg
    10621 taacgtgtaa agatctcacg atggaacacc ccggcatgcc gccgattttc cagagcttcg
    10681 atctcgaagt gaccccggga gaaaaagtcg ggatctatgc cagcacaacc gcggcgaaaa
    10741 cggcgctcgc acgggttctg gccggcttgg aagacccaac cagtggcgtc atccgatata
    10801 acggcgtcga tctgcgtcat cttgatctca attcgatcaa ccggtgtcgc gggttcatga
    10861 ttgactccca actatccttg ttcgagggta ccatcgagga caatatcgtg ttgggacgtt
    10921 cctatatccc ctacaacgac gttcggtggg ctttacgtgt caccgaactt gaggaggatg
    10981 tcgacgcgct tccccacggg ctgaaaactc acattcgggc accgggcaag atcctagctc
    11041 cgacacatat catgcgcatt ctcttgtcac gggctatcct ggcgcgtccg caaatgctga
    11101 tctttgacgg aatcctgcac aacctgcaac ctgccatgcg cgaaacgatt ctgcgtcggc
    11161 tctgttccaa ggacgaaccc tggtccgtca tctttgtgtc aaacgatccg aacttgacgc
    11221 cacacgtcga tcgtcgattg atcctcgagt aagcggaggt ttcacgtggg tctgtcttca
    11281 taccttcgat cgtacagacc gagcggtgca cagatcatca tcagcttgct gattgccggc
    11341 ctgggctggc tcagcgggca agcgctcagt caggtcgatc aagatatgcg gattatgtat
    11401 acggagtata cgctcggtgc ggcagacttg gcccacatct ctgcggacgt gatccgctat
    11461 cgtaatacca tcatccgtgc cctggaggcg gataacaaga aggactttga gcggattacg
    11521 gaatccctcc ctattcagcg cgcgaaaatt cagcacgcgg tggatcgcta tgcggcagca
    11581 ggattgcgcg tgtcccgaag cggacgaagc gagcccgaag atattgaagc ggtacgcagg
    11641 agtttggatc agtacttctc cgttgccagc aggaccattg atcttctgac gcaagtatgg
    11701 aacgccccat tgcaaccgga tcgcgaggca ttacggcgca aggccgaaga gcatgccgcc
    11761 gacaatggcg gccctaagat gattcaagtc agtctggcct tggaccgact gcttgatacc
    11821 gtcgccgatg tcgccaagga tatgcgggac gaagggacca agacgattca aaagacgagt
    11881 gtcctggtta ttggagggag tttcttcatc gccatactga atctcttcct tgcagcgccg
    11941 cggaggcggg atcgtaatcg agatgatacc gaggtccatc gcatgatcga agctccggat
    12001 gcccacgtat cacttccgct gggcaacgat cggcccaatc ccgtcctgcg aacagagtga
    12061 gcgacatccc gccacacaac ggtttgacgt tacttcgaaa ctgcaccacg ctcgacctcc
    12121 tgtaagagct tcgttcgttc ctcatcggtc agatctttcc atgtgttggg agccaatcct
    12181 gcaattgtaa tatgcatgat ccggacacgg tggagcgtca gcacccgata tccgagagcg
    12241 tggcacatgc gtcggatctg tcgattccga ccctccgtga ggatgatgcg aaaccgtgca
    12301 ggtcccagtc gttcgaccca acagggcttg gtccgacggc ctaagatcac gactccctgg
    12361 accattcgat caaggaatgc ctgatcgaac ggacgatcca ctgacacgtg atattcgcgt
    12421 tcatgaccaa actccgttcg aagtatctcg ttgacgatat cgccatcatt cgtcagaaga
    12481 atgagccccg atgagtcttt gtcgagacgg ccgatcggga aaattcgttc cggatggcca
    12541 atcgcggcga tgatgttccg agtcacatga gattcgcttg ttgtcgtcac cccgatcggc
    12601 ttgtgatact tgatgtagat ggccttgttt ccccacggaa tgacctgacc atcgcgagca
    12661 atgacgtcac cgacggcaat ctggtccccc agtttggcag ggcggccgtt gatagtgacg
    12721 cgaccgcctt caatcag
//
LOCUS       DATDNW010000292         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_513836, whole genome shotgun sequence.
ACCESSION   DATDNW010000292 DATDNW010000000
VERSION     DATDNW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513836"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1071)
                     /locus_tag="VI359_07055"
     CDS             complement(145..1071)
                     /locus_tag="VI359_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY6974045.1"
                     /translation="MAHLQQVEPSLNDVRDTVDQSIPSSMTVIPEASERSDPPAQAIE
                     RVYRHRLPVRLGHWLNVVCLFILIMSGLQIFNAHPALYWGDRSDRDKPLLSIRPMKTE
                     SGDVRGITTIFGHQFDTTGVLGYSNGSGRAFPAWATIPSARWLAMGRQWHLFFAWVFV
                     INGLLFMVYALISRHVTRDLAPTGQDFREIGKAINDHVRLRHPSGEEAKRYNVLQKLA
                     YMGVLFGLAPLIILTGLTMSPTIDTAFPWLLTIFGGRQAARTVHFIACFAFVGFIVIH
                     VSQVILTGLFNNLRSMTTGWFVVKHEGVRHEA"
     gene            complement(1242..1502)
                     /locus_tag="VI359_07060"
     CDS             complement(1242..1502)
                     /locus_tag="VI359_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974046.1"
                     /translation="MKKILLSLAAVVCLVGFASLSLADEMGKMKGEMKGEMKGEKDKM
                     KGEMKGKSEEMRGKHDEMKGDMKAEKDKMKGEMKGKMGEMGK"
     gene            complement(1620..>2624)
                     /locus_tag="VI359_07065"
     CDS             complement(1620..>2624)
                     /locus_tag="VI359_07065"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY6974047.1"
                     /translation="LVQVLRVGVDGTDKEINAAEYGQSPPGYDFLVIGHECFGRVLEV
                     GHNVTEFMPGDYIVPTVRRPGGSFYDRIGQYDMTTEDTYYERGINLRHGYLTEYFVDD
                     PEYIVKVPKGLKHVAVLLEPTSVIEKGIIQAYEAQRRLKVWRPKRAAVLGAGTIGMLA
                     AMSLRMRGLDVVSFGRNPAPYRNSELLAEIGAHYISTQDTSIKAAAEKYGPFDLMFEA
                     TGFAPIVFEAMEHLGKNGVLILSSVTGGGRNVQVPADMINLGFVLGNKVMVGTVNANR
                     EYFEAGVYDLSRAELEFPGWLSKFLTHPVAGLDNYQEMMQTLTTERSAIKVYVNVAYD
                     "
BASE COUNT          553 a          791 c          721 g          559 t
ORIGIN      
        1 tggacacgat cggtcagttt ctcggccttg ttgaggatcg atggaaacca actgctctgc
       61 gtcaaattgt cgcagccggc gagtgcgacc agacttgcgg ctccgagcgt gcccttcaga
      121 aattgccggc gttccatagc ggtcttaggc ttcatggcgg actccttcat gtttgaccac
      181 aaaccatccg gtggtcatgg agcgaagatt gttgaagagg ccggtcagga tgacctgcga
      241 cacgtggatg acgataaacc ccacgaaggc aaagcaagcg atgaaatgaa ccgtgcgcgc
      301 cgcctggcgg ccgccgaaga tcgtcaggag ccaggggaag gccgtgtcga tcgtcggaga
      361 catcgtcaga cctgtgagaa taatcagcgg cgccagtccg aagagcacgc ccatgtacgc
      421 caacttctgc aagacgttat agcgcttggc ttcctccccg gacggatgcc gcagacggac
      481 gtgatcattg attgccttcc cgatctcgcg gaaatcttgg ccggtcggag ccagatcgcg
      541 cgtaacatgc cggctgatca gtgcatagac catgaagagc aggccattga tgacaaagac
      601 ccaggcgaaa aagagatgcc actgccgtcc catggcgagc cagcgagcac tcggaatcgt
      661 ggcccaggcc gggaaggcgc ggccggagcc atttgagtag ccgagcacgc ccgtcgtgtc
      721 gaactggtgt ccgaaaatcg tggtgatccc gcgcacgtca ccgctctcgg tcttcatggg
      781 gcggatcgag agcaacggct tgtcacgatc ggagcgatca ccccaataga gtgcaggatg
      841 ggcgttgaag atctgcagcc cgctcatgat cagaatgaac aaacagacga cgttcagcca
      901 atgtccgaga cgaaccggga gccggtgccg atagacccgt tcgatcgcct gagccggcgg
      961 atcactccgt tcagaggctt caggtatgac ggtcatcgag gaaggaatcg actggtccac
     1021 tgtatcgcga acgtcgttca agctcggttc gacttgttgg aggtgtgcca tgggaggctc
     1081 ctcggtaaga gagtggttaa gattgttgtc cattgccttc cggtaagtcg cacggagccg
     1141 ccagtcctta caggcatccc ggacttgtcc agaaacagca aggcctgcgg tcgggatgtc
     1201 ccgagcgcag gcccgtgagg agcgagagca aggcgggcgt cttacttgcc catttctccc
     1261 atcttcccct tcatctcgcc tttcatcttg tccttctcag ccttcatgtc acccttcatc
     1321 tcgtcgtgct tgcccctcat ctcctcgctc ttgcccttca tctcgccttt catcttatct
     1381 ttctcgccct tcatctcacc tttcatctcg cccttcattt tgcccatctc gtctgccaac
     1441 gagagtgaag cgaagccgac cagacacacc actgctgcga gtgatagcag gatctttttc
     1501 atcgaggacc tcctgtgatg gtggcgcgag tgcgtgattg caccctgcgc gctacgaata
     1561 acgaagtcaa tgcggaaatc tcggaaccgg actcagcact aacgggtgcg accgcaggct
     1621 caatcatacg ccacgttcac gtagaccttg atcgcgctcc gctccgtggt cagcgtctgc
     1681 atcatctcct gatagttgtc cagccctgcc accggatgcg taaggaactt ggaaagccag
     1741 ccaggaaatt cgagttccgc gcgcgacaga tcgtatacac cggcttcgaa atattcccgg
     1801 ttagcattca cagtccccac catgaccttg ttgccgagaa cgaatcccag gttgatcatg
     1861 tcggccggca cctgaacgtt tcggccgcca ccggttacgc tggagaggat caagacgcca
     1921 ttctttccta aatgctccat cgcttcgaag acgatcggcg cgaagccggt ggcctcgaac
     1981 atcagatcaa aaggcccata cttctccgcc gccgctttta tggatgtgtc ctgtgttgag
     2041 atgtagtgcg cgccgatctc ggcgagcaat tcggaattcc gatacggagc ggggttccgg
     2101 ccgaagctca ctacatccag cccgcgcatg cgcaatgaca tcgctgccag catgccgatc
     2161 gtgcctgcgc ctaacacagc ggcgcgtttc ggccgccaga ctttcaaccg gcgttgagcc
     2221 tcgtaggcct ggatgatccc tttttctatc accgaggtcg gctcaagcaa cacagccaca
     2281 tgcttcaacc ctttggggac tttcacgatg tactccgggt catccacgaa gtattccgtc
     2341 agatatccat gccggaggtt aatgcctcgc tcgtagtacg tgtcctcagt cgtcatatcg
     2401 tactggccaa tcctgtcata aaagcttcct cccggacgcc gcaccgtcgg cacaatgtag
     2461 tcgcccggca tgaactccgt gacattgtgg ccgacctcga ggacacggcc gaagcattcg
     2521 tgaccgatca cgaggaagtc gtaaccggga ggggactggc cgtattcggc ggcgttaatt
     2581 tctttatctg tgccatcgac acccacccgc aacacttgga cgag
//
LOCUS       DATDNW010000293         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_513990, whole genome shotgun sequence.
ACCESSION   DATDNW010000293 DATDNW010000000
VERSION     DATDNW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513990"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VI359_07070"
     CDS             complement(<1..265)
                     /locus_tag="VI359_07070"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="catalyzes the formation of 2-amino-3-oxobutanoate
                     from acetyl-CoA and glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY6974048.1"
                     /translation="MAYHSFKKVIDQQLAEIRTAGLYKTERQILSPQGPEVRVVQGTV
                     LNLCANNYLGLANHPEITRAAVEGLKEHGYGMASVRFICGTQDL"
     gene            complement(347..1399)
                     /gene="tdh"
                     /locus_tag="VI359_07075"
     CDS             complement(347..1399)
                     /gene="tdh"
                     /locus_tag="VI359_07075"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY6974049.1"
                     /translation="MRYNQFMRALVKATAQPGLTMTTWPDPTPGVTDVVIRVKATSLC
                     GTDAHIYKWDEWAQGRITPPRIIGHELCGEVVETGSDVTLVKKGDYVAAESHLTCGTC
                     FQCRTGQAHVCKNYRILGIDRDGSFAEYISLPQGVLWKTSHDIPPEYACVQEPLGNAV
                     DAALAEEVAGRTVLITGCGPTGLFAAAIARTAGAAMIIASDVSEYRLDLAKQVGVDHV
                     LNAKTETAESLAATVRTITAGEGVDAALEMSGDPTALHHVFRAVKNGGRVTLFGIPTG
                     SVCFDLPNEIIFKGIRVYGVTGRRLFSTWYRLAGLFKAGLNIKPVITHTLPMTDFAKG
                     FDLIKSGQCGKVVLFP"
     gene            1474..2466
                     /locus_tag="VI359_07080"
     CDS             1474..2466
                     /locus_tag="VI359_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY6974050.1"
                     /translation="MKVPGFRCLQCMVGMLGVVVCLWAFESVTFPAASSSSTSPSSAS
                     KRWVQFDLDQEESAADILSGKPITLTVTLGGVAQGSTPIVAICESVHFQSQTITLEPD
                     TVPMVLKGTVTLEPIPPSRTSVPHKVARIQVTFARSRQDKLERIMRRIVYVTLESQEP
                     AGDSSDPPPLRPDESRNEDVILDEVQPGVEPVSTGKMAEEDLLPLPSPGEGAAYWQQI
                     SYLVSRSWSRHVRGIRHTPSGETVKVRFKLYPNGRAQLVEIEKGSGAREIDEAGIHTV
                     VSAQPFPPFPPELGEDAVDVHVRMRTGLRPKKRDVQSVASPTTKPDAPESPPKK"
     gene            2514..3128
                     /locus_tag="VI359_07085"
     CDS             2514..3128
                     /locus_tag="VI359_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY6974051.1"
                     /translation="MVRLYRHAGVLVALCQALIWHAGPAQAESARWNVDPDHSTIEFR
                     VSHMVVSKTTGRFMDYTGFIEMDAETGTVTAIEATIKTASVSTIHEKRDAHLRNPDFF
                     DVEKHPTMSYKMKSYKKTGETYTAIGDLTLRGVTKEITLTGNFNGTTKDPLGNTRAGF
                     NAEGKLNRKDFGMIWNKTLDSGGLVVGDEVQIRLDIECIKAKTP"
BASE COUNT          677 a          885 c          938 g          628 t
ORIGIN      
        1 ggagatcctg cgtcccgcaa atgaatcgaa cggaagccat gccatagcca tgttccttca
       61 acccctctac tgcggcccgt gtgatctccg gatggtttgc gagcccgagg taattgttgg
      121 cgcagaggtt cagcacggta ccttgcacca ccctcacctc cgggccttgc ggactgagaa
      181 tctgccgctc ggtcttgtac aggcccgctg tccggatttc ggccagttgc tggtcgatga
      241 ccttcttgaa cgagtgatac gccatatcgc tattcaggca ggaggcacgg ggcgagtggc
      301 aaagggagtt cttgccatac ttctcgcgct ctgcttcgag cctgtgtcac gggaatagca
      361 ctaccttgcc gcactggccc gatttgatca agtcgaaccc tttggcgaaa tcggtcatgg
      421 gcaaggtgtg cgtgatgaca ggtttgatat tcaggccggc tttgaagagc ccggccaatc
      481 gataccaggt gctgaaaagg cgccggcctg tgacgccgta gacgcggatt cctttgaaaa
      541 tgatttcatt cggcagatcg aagcagaccg atccggtggg gatgccgaac aacgtgactc
      601 gccctccatt tttgaccgca cgaaacacgt gatgcaacgc ggtcgggtcg ccggacattt
      661 ccagcgcggc gtcgacgcct tcaccagcgg tgatcgtccg aacggttgcg gcgagggatt
      721 ccgctgtttc ggtcttggcg ttgagcacgt gatcgacgcc cacttgcttc gccagatcca
      781 aacggtactc actcacatcc gaggcgatga tcatggccgc gccagcggtg cgcgcaatgg
      841 cggcagcgaa gagtccggtc ggcccgcagc cggtgatgag cacggttcgg ccggcgacct
      901 cctccgccaa cgctgcatcg acggcatttc ccaatggctc ctgaacgcag gcgtattccg
      961 gtggaatgtc gtgcgaggtt ttccagagaa cgccttgcgg caaactaatg tattcggcaa
     1021 aggagccgtc tcgatcgatt cccaggatgc ggtagttttt gcagacatga gcttggccgg
     1081 tgcggcattg gaagcaggtc ccgcacgtca ggtgcgattc tgcggcgaca taatcgcctt
     1141 ttttcaccag cgtcacatca gatcctgtct cgacaacctc tccgcatagc tcgtggccga
     1201 taattcgagg gggggtgatg cggccctgag cccattcgtc ccacttgtag atgtgcgcat
     1261 cggtcccgca aagggaggta gccttcacgc gaatcacgac atcggtgact ccgggtgtcg
     1321 ggtcgggcca ggttgtcatg gtcaatccag gctgggcggt ggctttcacg agcgcgcgca
     1381 tgaactgatt gtatctcata ccactcaatc gcgcaccctc atccgacaaa aacgggttgc
     1441 gcgagagcca gcgggctagg taggatgcgc ggtatgaagg ttccagggtt ccgatgcctc
     1501 caatgcatgg tcgggatgct gggagtcgtg gtctgtctgt gggcctttga atccgtgacg
     1561 tttcccgccg cttcctcctc atcgacgtca ccctcatcag cctcaaaacg atgggtgcaa
     1621 ttcgatctgg accaagaaga atctgccgcc gacattcttt ccggaaaacc catcacgctg
     1681 accgtgacgc tcgggggtgt ggcgcaaggg tccaccccga tcgtggccat ctgtgaatca
     1741 gtgcactttc aatctcagac gattacgctc gagccggata cggtgccaat ggtgctaaaa
     1801 gggactgtga cgcttgagcc gatcccccct agccgaacgt cggtaccgca caaggtggca
     1861 cggattcaag taacgtttgc ccgatcccgg caagacaagc tcgaacggat catgcgccgg
     1921 atcgtctatg tgacgttgga gtcacaagag ccggcgggcg actcttcgga tccgccgccg
     1981 cttcgaccgg acgaatctcg caacgaggat gtgatcctcg atgaagttca gccgggcgtc
     2041 gaaccggtgt cgaccggtaa aatggccgag gaggacctgc tgccattacc ttcgccaggt
     2101 gagggcgcgg cctattggca gcaaatcagt tatctcgtca gtcggagttg gagtcgccac
     2161 gtacgcggga ttcggcatac gccgagcgga gaaacggtca aagttcggtt caagctctac
     2221 cccaacggtc gggctcaatt ggtcgagatc gaaaaggggt ccggggcgcg tgaaatcgac
     2281 gaagcgggga tccacactgt cgtgagcgcg caaccgtttc ctccgtttcc gccggagttg
     2341 ggagaagacg ctgtggatgt gcatgtacgg atgcgcaccg gcctccggcc gaagaagcgc
     2401 gacgtacagt cggtcgcaag ccccaccacc aaaccagatg ctcccgagag tccgcccaag
     2461 aagtagccaa gcgtccagag attcacaggt ctattcggag ggaggcagac acgatggtga
     2521 gactatatcg gcacgcggga gtcctggtgg ctctttgcca ggcactgatc tggcacgctg
     2581 gcccggcgca ggccgagtcg gcccgttgga atgtcgaccc cgatcattca acgatcgaat
     2641 ttcgcgtctc gcacatggtc gtctccaaga cgacgggccg gtttatggac tacaccggct
     2701 tcattgagat ggatgcggaa accgggactg tgacagccat cgaggcgacg atcaagaccg
     2761 catcagtcag caccattcat gaaaagcggg acgcccatct gcggaatccc gatttcttcg
     2821 atgtagagaa gcatccgacg atgagctaca agatgaagag ctataagaaa accggtgaaa
     2881 cctataccgc gattggagac ctcacattgc gaggtgtgac caaggaaatc accttaaccg
     2941 ggaatttcaa tgggacgacg aaagacccat tgggcaatac gagggccggc ttcaatgcgg
     3001 agggaaaact gaatcgcaag gacttcggca tgatctggaa caaaacgctc gacagcggcg
     3061 ggctcgtggt cggtgacgag gttcaaatcc gactcgatat cgaatgcatt aaagcaaaaa
     3121 caccgtag
//
LOCUS       DATDNW010000294         3394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_805327, whole genome shotgun sequence.
ACCESSION   DATDNW010000294 DATDNW010000000
VERSION     DATDNW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805327"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VI359_07090"
     CDS             <1..980
                     /locus_tag="VI359_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974052.1"
                     /translation="VYREFPATIQPNENELAEVTTLIRGRVVKVYVDVGQDVKKNTLL
                     TMLHSTDLGLAEGAYLKARAKLHEAELVYERAKDLHEHKAVSLAELQRREAEMKTARA
                     EARETQNRMELLGVQAQEIERLEREHTIKADVPLRAPFDGRVIMRNITRGEVVETQQK
                     LFTVADLSNVWVIGNVPEKDVQFIRKDQKVEVIAAAYPHGIFPGTITYVSDILDPATR
                     TMRLRVTVPNPDRLLKPEMFALVRVYAALKPDVVTIPLAAVQNGSAGKIVFVQQAPDE
                     YAVRTIKLGDEQGETVVVEEGLQEGERVVVKGSFTLKSEMERHKIEPAP"
     gene            977..>3394
                     /locus_tag="VI359_07095"
     CDS             977..>3394
                     /locus_tag="VI359_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6974053.1"
                     /translation="MIASLLEFSLRQRILVLGLAGLLAIAGVYAFQAIPIDAYPDVTN
                     IQVQVLTDAPGLSPVEVERFITYPLELQMTGLPGLAEIRSLSKFALSQITVVFNDDID
                     IYFARQLVLERILAAKEKLPEGMEPVMGPLSTGLGEVYQYYVEGPKAADSDPTVVEAE
                     LTDQRTMQDWVLRPLLKSVPGVIDVNAMGGFVKQYQVLVDPAKLRKYDLTLHEVYDAV
                     AKNNANAGGNVLERHAERSIVRGLGLIKTLSDIESIIVKESGGTPVFVRDVAEVRIGH
                     AVRHGAVVLNGEREVVAATVLMLRGGNAREVVQAVKARMEDLHQNHVLPDGTTIKPFY
                     DRIDLVTEAINTVRDALLEGVLLVIFVFFFFLGHVRSAIIVTVTLIVTPLVTFIVMQR
                     LGLSANLMTLGGLAIAIGEIADGSLVVVENVYRHLADNNRAPQKSKLEVILHATKEVG
                     RPILFGILIISVVFLPLMTLHGMEGKMFAPLAYTLVIALLVSVVVTLTLSPVLASLLL
                     RGNHPQETRLTIWMKQRYRPVLRWTLEHRSPVLLGSSAVLLASLVLVPFVGREFIPIL
                     LEGALTPQVVRLPSVSLPESIEIEKQTHRAMLEFPEVKMAVSKIGRPDIAVGPEEPNE
                     SDPIVSLHDRSTWTTARTQPELTDAIRKKLAGIPGISVLMSQPIQERVDELISGIRTE
                     CAIKLFGDDLEVLKQKADEIAALMHGIKGVKDVKVEQIAGQPYLTVDIDRQKIARYGI
                     NVADVQEIITTAIGGKSATHVYEGERRFQLILRFPEASRNSVGAIGEIRVKSASGALI
                     PMGDLAKI"
BASE COUNT          722 a          975 c         1033 g          664 t
ORIGIN      
        1 gcgtataccg cgagtttccg gctaccattc aacccaacga aaatgaattg gccgaggtca
       61 caacgctcat tcgcgggcgg gtcgtaaagg tttatgtgga tgtcggccag gacgtgaaga
      121 aaaacacatt gctgaccatg ctccatagca ctgatcttgg gctcgcggaa ggggcttacc
      181 tgaaggccag ggcaaagctg catgaagcgg aactcgtgta cgagcgcgcc aaggacctgc
      241 atgagcataa agccgtaagc ttggccgagt tgcagcgccg cgaagcggag atgaagacgg
      301 cgcgggccga agcgcgagaa acgcagaacc gcatggaact gctcggcgtg caagcgcagg
      361 agatcgagcg tctggagcgc gagcacacga tcaaagccga tgtgcctctg cgcgcgccct
      421 tcgacggtcg ggtcatcatg cgcaacatca cacggggtga ggtcgtcgaa acgcagcaaa
      481 aattattcac cgtcgcggat ctttcgaatg tgtgggtcat cggtaatgtg ccggaaaagg
      541 acgtgcagtt catccgaaaa gatcagaagg tggaggtcat cgccgcagcc tatccccatg
      601 gaatctttcc cggaaccatc acctatgtca gcgacattct agatccagcc acccgaacga
      661 tgcgcctgcg ggtcacggtg ccgaaccctg atcgactcct caagccggaa atgttcgccc
      721 tcgtccgggt ctatgcagcg ctgaaaccgg acgtcgtcac gatccctctg gcagccgtcc
      781 agaacgggtc tgccggcaag atcgtgttcg tgcagcaagc accggacgaa tatgcggtca
      841 ggaccatcaa gctgggcgac gaacagggtg agacggtggt cgtggaagag gggcttcaag
      901 aaggcgaacg agtcgtggtc aaaggatcgt tcaccctcaa gtctgaaatg gagcggcaca
      961 agatcgagcc tgccccatga tcgcctcact gttggaattc tcgctccgcc aacggatttt
     1021 agtcctcggg cttgcaggcc tcctcgccat cgcgggagtc tacgcgttcc aggcgattcc
     1081 gatcgacgcc taccccgatg tgaccaatat ccaggtgcag gtactgaccg acgcaccagg
     1141 tctctcgccg gtcgaggtgg aacgattcat cacctatccc ctcgaactcc agatgaccgg
     1201 cctgcctgga ttagcggaga tccgatccct gtccaaattc gccctgtctc agatcaccgt
     1261 cgtcttcaac gatgacatcg atatctactt cgctcgtcaa ttggtgctcg aacggatcct
     1321 ggcggcaaaa gagaagttgc ctgaggggat ggagccggtc atggggccgc tctcgaccgg
     1381 ccttggcgag gtctaccaat attatgtgga aggaccgaag gcggccgact ctgatccgac
     1441 ggtcgtcgag gcagagctga ccgaccaacg cacgatgcag gactgggtgc tccgtccctt
     1501 gctgaagagc gtgcccggtg tcatcgatgt gaacgccatg ggcgggttcg tgaaacaata
     1561 tcaagtgctc gttgatccgg ccaagctccg gaagtatgac ctcacgttgc atgaggttta
     1621 cgacgctgtg gcgaaaaaca atgccaatgc cggtggcaac gtgctggaac gccacgccga
     1681 acgttcgatc gtgcgaggac tcggcctgat aaagaccctc agtgatatcg agtccattat
     1741 tgtgaaagag tcgggcggga caccggtctt cgtccgcgac gtggcggagg tccgtatcgg
     1801 ccacgcggta cgtcacggcg ccgtggtgct gaacggggag cgggaagtcg tggcggcgac
     1861 cgtcctcatg ttgcggggcg gcaatgcccg cgaggtggtc caggcggtga aggccaggat
     1921 ggaagatttg catcagaatc atgtcttgcc ggacggcacg acgatcaaac ccttctacga
     1981 tcggatcgat ctcgtgacgg aagcgatcaa tacggtgcgc gacgcgttgc tcgaaggtgt
     2041 acttctggtc atcttcgtgt tcttcttctt tctcggccac gtgcggagcg caatcatcgt
     2101 cacggtcact ctgatcgtca ccccccttgt cacgttcatc gtcatgcagc gactcgggct
     2161 ttcggccaat ctgatgacgc tcggcggcct cgccattgcc atcggcgaaa tcgcggatgg
     2221 ctcgctcgtc gtcgtcgaga acgtctatcg acacctggcg gacaacaatc gcgcgccgca
     2281 gaaaagtaag ctggaggtca ttctccacgc cacgaaggaa gtgggccggc cgatcctgtt
     2341 cggtattctg atcatcagcg tggtctttct cccgctcatg acgcttcacg gcatggaggg
     2401 caagatgttc gccccgctgg cctatacgct ggtgatcgcc ctcttagtct cggtggtggt
     2461 cacgctcacg ctctcgccgg tcttggcgtc acttctgtta cgcgggaacc atccgcagga
     2521 aacccggctc accatttgga tgaagcagcg gtaccgaccg gtgctgaggt ggacactcga
     2581 gcaccgcagc cccgtcctgc tcggctcgtc ggcagtgctc ctagccagcc ttgtcctcgt
     2641 cccgttcgtg ggccgtgagt tcattccgat cctcctcgaa ggggccctca cgccccaggt
     2701 tgtccggctg ccgagcgtct cgctgcccga gtcgatcgag attgagaaac agacgcatcg
     2761 agccatgctg gagtttcccg aagtgaagat ggccgtgagc aagattggga gacccgacat
     2821 tgctgtcggt ccggaggagc cgaacgagag cgatcccatc gtatcgctcc acgaccggag
     2881 tacctggacg accgcgagga cacagccgga gttgactgat gccattcgga aaaaactggc
     2941 ggggatccct gggatttcgg tactgatgag ccagccaatt caagaacggg tggatgagct
     3001 gatctcaggc atccggaccg aatgcgcgat caagttgttc ggcgatgatt tggaggtgct
     3061 caagcagaag gccgacgaga tcgctgcact gatgcacggt atcaagggtg tgaaggacgt
     3121 caaagtcgag cagatcgccg ggcagcccta tctcaccgtg gatatcgacc ggcagaagat
     3181 tgcccgctat ggcatcaacg tcgccgacgt gcaggagatc atcacaaccg ccatcggcgg
     3241 caagtcggcc acacatgtct atgaaggcga acggcggttc caattgatcc tgcgatttcc
     3301 ggaagcctct cgcaacagtg ttggcgccat cggagagatt cgggtgaagt cagcgtcggg
     3361 cgcgttaatc ccaatgggcg atctggccaa gatc
//
LOCUS       DATDNW010000295         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_805331, whole genome shotgun sequence.
ACCESSION   DATDNW010000295 DATDNW010000000
VERSION     DATDNW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805331"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VI359_07100"
     CDS             <1..580
                     /locus_tag="VI359_07100"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY6974054.1"
                     /translation="LEQLSARLQDQSLKLASVQRELAERERTLFELREDMHKRTLDVA
                     ELKDQLIQREAELDDLHNQLAATSKRGRTTQDELAALLRMPDVQAVSLSGADRAKGAG
                     GVLLFDPRTQKIWLYAVNLPECPTGTTYQLWAITDKPTSIGTFHMDSGETARMLVKRL
                     PDFSKTKQFAISLEPSGGRPQPTGAVYLTGSL"
     gene            673..1578
                     /locus_tag="VI359_07105"
     CDS             673..1578
                     /locus_tag="VI359_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974055.1"
                     /translation="MLLLRRILRLVTFAVLLLVGAAPNTWALEKYGRPLPEMEGAHAQ
                     ETEQWLRGYLLTSAFVHNPSFAARPDNTGLVGLRHMIHLETDIYKEYLQFYTDQNFFS
                     DRNNGWIILSEWDSVAAFTGTINNWGWRLQYERDAPLDRSGTKQIYADTLVTYSAPDA
                     QQWTWWRSVFPQQNLNFYAGAGWLFHNHDYFARPDNSGRALFRYVAHADLDLYRNRVV
                     LFADTNFFTDREASNVVNPSELDLVIGLAFRYDKAELAVIHEQDMPIDRTGLVQRYIA
                     IQLRYEFEWTKSSSTPSTARAFRHQ"
     gene            1663..2157
                     /gene="tadA"
                     /locus_tag="VI359_07110"
     CDS             1663..2157
                     /gene="tadA"
                     /locus_tag="VI359_07110"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY6974056.1"
                     /translation="MNASLAQDEQMMRVALEEAAKAPAIGEIPIGAVIIRGGEILAKA
                     HNYREIWQDPTAHAEIVAIRAAASTLGSWRLTETTLYVTVEPCAMCIGAIILARIPRL
                     VFGTRDPKAGACGSVFDLATDSWLNHRVLVTGGTLEGECQALLQHFFRGLRAESVQTS
                     DPST"
BASE COUNT          510 a          676 c          599 g          509 t
ORIGIN      
        1 gctggaacag ctctcggccc gactgcagga ccagtctttg aaactggcat ccgttcaacg
       61 agagctggcg gaacgcgagc gcaccttatt tgaactccgc gaggacatgc acaaacgcac
      121 ccttgacgtc gctgagttaa aagaccagct cattcaacgc gaagctgaac tggacgatct
      181 ccataaccaa ctggcggcca cttctaagcg aggacgaacc acccaagatg aattggctgc
      241 tctgttgcgc atgccggatg tccaagcggt gtcactctct ggggcagata gggccaaagg
      301 agccggtgga gtcctcctat tcgacccccg cacacaaaaa atctggctct acgcagtgaa
      361 tcttcctgaa tgtccgaccg gcacgactta tcagctttgg gccatcacgg ataagcccac
      421 gagtatcggt acatttcata tggacagcgg cgagacagcc cgcatgttgg tcaagcgcct
      481 gcccgatttt tcgaaaacca aacaatttgc catcagtttg gaaccttctg gcggccgccc
      541 tcagccgacc ggcgcggtct atctcacagg atccctctag ttcgcattct gcacttgacc
      601 tgcccggcag gcctcgctac aaccgcccag gccttgcctc attgatcagc ctgttcgtag
      661 gaaacttgca cgatgctttt gcttcgccga atattgcggc ttgtcacgtt cgctgtcctt
      721 ctgctggtgg gcgctgcgcc taatacgtgg gctcttgaga aatatgggcg tcccttgccg
      781 gaaatggaag gagcccacgc tcaagagacg gaacaatggc tccgggggta cctcctgacc
      841 agcgcatttg tgcacaatcc ctctttcgca gcgcgaccgg acaataccgg actggttgga
      901 cttcgacata tgatccatct cgagacagac atttataaag agtatctcca gttctatacg
      961 gatcagaact ttttctctga tcggaataat ggatggatca ttctgagcga atgggattcc
     1021 gtggctgcat tcaccggcac gatcaataat tggggatggc ggctccagta tgagcgcgat
     1081 gccccgttgg accggagcgg caccaagcag atttacgccg atactctcgt gacctattcc
     1141 gcgccagatg cccaacaatg gacgtggtgg cgttcggtct ttccccaaca gaatcttaat
     1201 ttttacgccg gtgccggatg gttattccac aaccacgatt acttcgccag accagacaat
     1261 tcaggccgtg ccctgttccg ctacgtcgcc catgccgacc tcgacctcta tcggaatcgt
     1321 gtggtgctgt tcgccgatac caatttcttt accgaccgtg aagcctccaa tgtggtcaac
     1381 ccgagtgaac tcgatctggt gatagggctt gctttccgat atgacaaagc cgaactggct
     1441 gtcatccacg aacaggatat gccgatagac cgcaccggcc tcgtccagcg atacatcgcc
     1501 attcaactgc gttacgaatt tgaatggacg aaatcgtcaa gtacgcccag caccgcccgc
     1561 gcatttcgcc accagtaacc tctgacttct tctcagcgaa tcagccatgg taagatacca
     1621 acccggcaac agagtagttt cgaacgacca tcgcgttttc cgatgaacgc ctctttggca
     1681 caagacgagc aaatgatgcg agtcgctctg gaggaggctg ccaaagcccc cgctataggg
     1741 gagatcccga ttggagccgt catcattcgt ggaggcgaga ttcttgcgaa agcacacaat
     1801 taccgtgaga tctggcagga cccgaccgct cacgcagaaa tcgtggcgat ccgggctgct
     1861 gcgtcgacac tcggctcgtg gcggctgacc gagacgacac tgtatgtcac agtggagccc
     1921 tgcgccatgt gcatcggcgc catcattctt gccagaattc cacgtctggt gttcggcacg
     1981 cgcgatccga aggccggcgc ttgcggctca gtatttgatc tggcgacaga ttcctggctc
     2041 aatcatcggg tattagtcac gggaggaacg cttgaggggg aatgtcaagc tctcttgcag
     2101 cattttttcc ggggccttcg ggcagaatcg gttcaaacaa gcgatccctc aacgtagccg
     2161 ccagtgagtg ttacgtacag agtgtcactt tccacccact tccctctgcg gctacggctc
     2221 caacccaacc ttcttccagc ggaagaaatt gaatggttgg cggagcgctg tccggcggac
     2281 tgccttcgct gacg
//
LOCUS       DATDNW010000296         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1138779, whole genome shotgun sequence.
ACCESSION   DATDNW010000296 DATDNW010000000
VERSION     DATDNW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138779"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VI359_07115"
     CDS             <1..1086
                     /locus_tag="VI359_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6974057.1"
                     /translation="GGYPQFYDVKITQLVEQVNPGGQVWNVRVGRKHHAPYGVFEGMT
                     IFDAGAKVGQAAIGYIPTDQEWRFVNIYEDTATSMRSLVEGIDKSGFSRDEPWRLTGS
                     SLPEHETFFFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQNRCRGYKKCVEQ
                     CPFKKPMYRGTTRVSEKCIACYPRIEGKDPLTGGEPMETRCMAACVGKIRMQSLVRIG
                     EDGLWAEDRWHPLYYAIRVEQVALPLYPQWGTEPNGYYIPPRHSPRGYARQMFGPGVD
                     NAIEKYLVPSRELLAVLQLWRASQQIIFRYDVIPGPKVFETQIHGKRFEMYNDTVLGF
                     NKSGKEVARIQVEEPIYVRPAERVNWL"
     gene            complement(1199..>2486)
                     /locus_tag="VI359_07120"
     CDS             complement(1199..>2486)
                     /locus_tag="VI359_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY6974058.1"
                     /translation="TSVVVFLGGVMGWMILMPAYGLMNGLPPDLTELAAAMTIWSGQI
                     RYVGIGAMLTGGLWTLTKLREPVWKSLQTLRDSYRASGPSRSGAVVPRTEQDASLLWI
                     VVPFLLSLVPMAWIYTTVVGSLLIGLLMTIVMALAAFLFSSVAAYMAGLVGSSSNPVS
                     GVTIATIMCAALLLVLFMGSGHPAGPAAALVIGAVVCCAAAMGGDNLQDLKTGHLVGA
                     TPWKQQIMQVVGVLTGAMVLVPVLSLLQAKYGIGEPTISHPHPLSAPQATLMASLTQS
                     VFGAGLPWPLVGLGAAIGILVILVDRRQEARGREFRLPVLAVALGIYLPLKLSAAIFV
                     GGVISAFAKRAAAKGDDLSRLGLLFAAGLITGEALMGIMLAIPIALAALWPSLSPDPF
                     TLFTSPPFGGWPGLLIVALVGLALYRMASGTMRKRR"
BASE COUNT          539 a          770 c          765 g          412 t
ORIGIN      
        1 ggcgggtatc cgcagttcta cgacgtgaag atcacgcagt tggtcgagca ggtgaacccg
       61 gggggccagg tgtggaacgt gcgggtcggc cgcaagcacc atgcgcccta cggggtgttc
      121 gaagggatga ccattttcga cgccggggcc aaagtgggcc aggcggcgat cgggtatatt
      181 ccgacggacc aagagtggcg gttcgtcaat atctatgagg acaccgcgac ctcgatgcgg
      241 tccttggtcg aaggcatcga caagtccggc ttctcccgcg acgaaccctg gcgcctcacc
      301 ggcagcagct tgccggagca tgagacgttc ttcttctacc tgcagcggat ctgtaaccac
      361 tgcacctatc cgggctgctt ggcggcgtgt cccagaaagg cgatctacaa gcggccggaa
      421 gacggcatcg tgttgatcga ccagaaccgc tgccgcggct acaagaagtg cgtggagcag
      481 tgtccgttca agaagccgat gtatcggggc accacacgcg tgagtgaaaa atgcatcgcg
      541 tgctatcccc gcatcgaagg caaggacccg ctgacgggcg gcgagccgat ggaaacgcgc
      601 tgtatggcgg cctgcgtcgg gaagatccgc atgcagagcc tggtgcgcat cggcgaagac
      661 ggcctgtggg cggaagatcg ctggcatccc ctgtactacg ccattcgggt ggagcaggtg
      721 gcgctcccgc tgtatccgca gtggggcacc gagcccaacg gctactacat cccgcctcgg
      781 cacagccccc gcggctatgc ccggcagatg tttggcccgg gcgtggacaa cgccatcgag
      841 aagtacctcg tgcccagccg ggaactgttg gccgtcctcc agctctggag agccagccag
      901 cagatcatct tccggtacga cgtcattccg gggccgaagg tgtttgaaac ccagatccac
      961 ggcaaacgct tcgagatgta caacgatacc gtgctgggct tcaacaagtc gggcaaggaa
     1021 gtggcgcgga ttcaggtcga agagccgatc tacgtcagac ccgcggaacg ggtgaactgg
     1081 ctctaagatc aatcagctga tcgttgggag aggcgttatc tctcccaacg atcactggtg
     1141 acgctctaag cggagtttca ttcagccttc ggctcggcag cttgtgcccc acccaatctc
     1201 atcgcctctt ccgcatagtg ccgcttgcca tccgatagag ggccaatccc accagtgcca
     1261 cgatcaagag accaggccag ccgccgaacg gaggcgaggt aaagagggta aacggatcgg
     1321 gactgaggct gggccacagc gccgccagcg caatcgggat cgccagcatg atgcccatca
     1381 acgcttcacc ggtaatcaag cctgccgcga acaataatcc aagccgtgac aggtcatcac
     1441 ccttcgctgc cgctctcttg gcgaaggccg agatcacgcc acccacgaaa atggcggctg
     1501 agagtttgag cggcaagtag atgcccaagg ccacggcgag cacgggcaaa cgaaactcgc
     1561 gtccgcgcgc ttcttgacgt cgatccacga gaatcacgag gatgccgatc gctgcgccca
     1621 atccgacgag cggccaaggc agtccggctc cgaatacgct ctgcgtgagg ctggccatca
     1681 gcgtggcctg cggtgcactc agagggtggg ggtggctgat cgtcggttct ccgatcccgt
     1741 acttggcttg gagcaacgag aggaccggaa ccaggaccat ggcgccggtc agcacaccga
     1801 cgacctgcat gatttgctgc ttccatggtg tcgctccgac cagatgaccg gttttcaaat
     1861 cttgcaaatt gtcgcctccc atcgccgcgg cacaacagac gaccgctccg atcacgagcg
     1921 ctgccgccgg tccggccgga tggccggatc ccataaacag gacgagtaaa agagcggcac
     1981 acataatcgt ggcaatcgtg acgcccgaga cgggattgct ggaactcccg accaacccgg
     2041 ccatgtaggc cgcgacggag gagaagagaa acgctgcgag cgccattacg atcgtcataa
     2101 ggaggccgat cagaagactg cccacgaccg tggtatagat ccaggccatc gggactagtg
     2161 acagaagaaa tggcacgacg atccagagga gcgacgcgtc ctgttccgtc ctgggcacga
     2221 cggccccgga tcgagacggc cccgatgcac gataactgtc ccggagcgtc tgcagactct
     2281 tccacaccgg ctcgcgcagc ttcgtcaacg tccacagtcc gcctgtcagc atggcgccga
     2341 taccgacata gcggatctgg ccgctccaga tcgtcatcgc ggcggccagt tcggtcagat
     2401 cgggcggcag accattcatg agtccatacg caggcatcaa gatcatccat cccatgacgc
     2461 cgcccaggaa aaccaccacg gaggta
//
LOCUS       DATDNW010000297         5821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_248578, whole genome shotgun sequence.
ACCESSION   DATDNW010000297 DATDNW010000000
VERSION     DATDNW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248578"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1154)
                     /locus_tag="VI359_07125"
     CDS             complement(180..1154)
                     /locus_tag="VI359_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974059.1"
                     /translation="MPVKYLFITIAVCWTFFQLSGLIWSSKGQVPSETLARLLEPPPQ
                     YISDPRQNGYYYLLGLTVAPSLDPAWVGYDIWHETAAKPVTATFDYDQSGRSDARLAV
                     VASFLSPIWDSDDLFAELRRRESALRASVNQYSLFLTRYDHWLGMKFEDQGFAHRAIP
                     HCEDILLVHRLYLADAFSQRLTLGMNRLQRDISAWRLILRDARTLSMKVFAQVMIADD
                     VKLLSKVFARPIIDKSLLSIGASLVTPLTPAEYSLRWPIRHQLALGVQRDRLNGLTGA
                     EGEGTGETYVQWLVEAAALPSQSFRRVEHPASSSFLGIPLQTQRTWDT"
     regulatory      1347..1554
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1855..2748
                     /locus_tag="VI359_07130"
     CDS             1855..2748
                     /locus_tag="VI359_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY6974060.1"
                     /translation="MKRRQQGILTGMPFMAHVSSRSFVDDAGRKIYLAKTPARIVSLA
                     PSVTEMLFAIGAADQIAGVTQFCNYPPEALKKPKVGYANPNLESLVALQPDLVVGPIE
                     FLKPDLVLKLEELKIPVFALTEKNVEDIFIHVQTLGRMLDRSTTANAVAMELRQQVAQ
                     IKQRTERFSPVRVLYVLNSEPLITVGPGSFIDQLIVLAGGANVAAKSGTPYPRLSMET
                     VLHDDPEVLVFPVGESEGISDSEQQSWRQWSTITAVKQGRLHQIPADLLNRPGPRVVR
                     ALELLADILHPKRTSSSRENQ"
     gene            2750..3907
                     /locus_tag="VI359_07135"
     CDS             2750..3907
                     /locus_tag="VI359_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY6974061.1"
                     /translation="MNPVTPVPPPPAHVESASIDHPALTTDTARALSQWMKTGSILTL
                     HRWLLVIGGLIVASVVVLGVCLEFGAIPVEMRTVVGAILALFQREQIDVDGLGTPGII
                     MVQIRLPRVLMGFLVGGSLAGVGVTLQALLRNPLADPYVLGVSSGAALGASLAMLLGI
                     GTSFALLPILPLFGFLGGLLSLVLIYRLAQSQGRLPVHSLLLAGVILNAILTALIMFI
                     TTIMDPNRSAGLMAWLMGSLTAPSYPALAVFVLYVVVVLAILMHKAQMLNVMTLGEDI
                     ARSLGIETEWVKKQLFVLTALLTGAVVSVSGMIGFVGMVIPHVVRMLVGSDHRLLFPA
                     SALVGGMFLVVSDTIARTLLSPSEIPVGIVTALAGGPFFLYLLLCRKDRMS"
     gene            3909..4790
                     /locus_tag="VI359_07140"
     CDS             3909..4790
                     /locus_tag="VI359_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974062.1"
                     /translation="MAKSTIELAKSWLSEGSSALQVDNLFFRYRTTSEQARTWALEQV
                     SFQVAAGEILGIVGPNGSGKSSLLKVLARLVSSHSGTILLGGQSIQQFSQHDIARIVA
                     VVPQEYVQVFPFTVAETVLMGRFPHRTPRFWSIGFGDDTKKDLVCAHQAMIETDVLLL
                     ANRLVSDLSGGERQRVVIARALAQEPKILLLDEPTAFLDINHQIEICSLISRLRAERR
                     LTVVLVSHDLNVASQYCDRVLMLKEGRLYRIGSPAETIRPDVLRMVYGCEVVVDAHPQ
                     TGRPRVTMPVDAGSAEK"
     regulatory      4864..5036
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5063..>5821
                     /locus_tag="VI359_07145"
     CDS             5063..>5821
                     /locus_tag="VI359_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY6974063.1"
                     /translation="MPFLAVWWFSCLSLLGLLLPADEVFAQEVAFADQEEIVETPEVV
                     VTATKTETPVKQVTSAVEIITGDQMKQRNVKTVAEALRWAQGLAVFQSGGPGTIVDVR
                     VRGGTPEQTLVLIDGAIVNSATVGTYDFANLTTDNIERIEILRGSQSMLWGSDAMGGV
                     INITTKRGRDIPSISAFSEYGSFNTIREGANLNGKKGPVDFSGSISRWDTASFSSVNY
                     RRGATERDGYHNWQGSVRLGAELPKDGRLEFSFRW"
BASE COUNT         1259 a         1457 c         1733 g         1372 t
ORIGIN      
        1 agtcgtgcgt cggtttccat caattgttga tggaacaggt cccattccgg ttcaaacgtg
       61 gccgagctga atgcgttctc cagaaaccct cgtcttgtcg agcgggagac ctcgcgaaag
      121 gtcggcatgg agccgcgacc agattcggat gcagtaatga tcgcgtcata atagctcgcc
      181 taggtgtccc atgtacgttg tgtctggagt ggaataccga gaaaagagct cgacgcaggg
      241 tgctcgactc gacgaaaact ttgtgaggga agcgcggctg cctcaaccag ccattgcaca
      301 tatgtctcgc cggttccttc accctcggcg ccggtcaacc cgttgagtcg atcgcgctga
      361 acaccaagtg cgagctgatg tctaatgggc catcgaagcg aatactcggc gggagtcagg
      421 ggagtcacga gacttgcacc gatggagaga agcgatttgt cgatgattgg acgggcgaac
      481 acttttgata agagcttcac gtcgtcagcg atcataactt gagcgaacac tttcatcgac
      541 agggttcttg cgtcacgaag gatcagtctc caggcactga tgtccctttg gaggcgattc
      601 atacccaagg taagccgctg ggaaaaagcg tcggcgagat acagacggtg caccagtaga
      661 atgtcttcgc aatgcgggat agcccgatga gcgaatccct gatcttcaaa tttcattccg
      721 agccaatggt cgtagcgcgt gaggaagagg ctgtattgat tgaccgatgc gcgcaatgcc
      781 gactcacgtc gtcgcaattc cgcgaataga tcatctgagt cccaaatagg tgagagaaaa
      841 cttgcgacga cggccaagcg ggcatctgag cggcctgatt gatcgtagtc aaacgtagcc
      901 gtgacaggtt ttgcagcagt ttcgtgccaa atgtcatagc ccacccacgc cggatcaagc
      961 gacggagcga cggttaaccc cagcaggtaa tagtagccgt tctgacgggg atcggaaatg
     1021 tactggggtg gaggctccag aagtcgcgcg agcgtctcac tcgggacttg tccctttgaa
     1081 gaccaaatga gtcccgagag ctggaagaac gtccagcaaa cagcaattgt tatgaagaga
     1141 tattttactg gcatctacgt agcatgtcct ggtgatgctg gattcatggt gaccgggccg
     1201 attgtgccca gcagcggtct gctctaattt cagcaaggct tatgccgagc gcggtgggag
     1261 caagttatcg ttatctggtc gcttttgagg gtctcaggct ggtgtcggac gcacgaatcg
     1321 tgcaagcaag aaagagacgg acaataatga acttgacaaa tttcggcgca gtctcctaga
     1381 gtgcgtgcac attgtgactc ttgggtaaag ggaagagggt gcaactccct cgcggtcccg
     1441 ccgctgtgaa tggggacgaa acccgtgaga gccactgtcc gttacaggat gggaaggcac
     1501 ggggagtagg gtgaaccata agccagaaga cctgcccaag cagatgactt tcgttccttc
     1561 gtgggctggg gaacgggcga ggatgaagaa gcgggtgcaa tcctcagggt ctttctcagg
     1621 ccttggggat tttttatttc gagcgcgttg aaacaggttc cccgcgtcgt tctcgcgtcg
     1681 ctcacagctt gcgacgtacg gacaactgta cgtctcggat gttcgctcgc tgtggccttg
     1741 cggagaagac tgtttgaacg tcctgtacga cctgattggg tcatcgggat tttcgtctgt
     1801 cttctttggt tggatgttgg tgctgttctg gcccatgaca ccgaggtcgt gaaaatgaaa
     1861 cgacggcagc agggaatttt gaccgggatg ccgttcatgg cacacgtctc ctctcggtca
     1921 tttgtcgatg atgccggccg caagatctac ctcgcgaaga ctccagcccg catcgtctcc
     1981 ctggctccta gcgtgactga aatgctgttt gcaattggag ctgctgatca gattgccggc
     2041 gtcactcagt tttgcaatta ccctcctgag gcattgaaga aaccgaaggt cggatatgcc
     2101 aatccgaatc tggaatcgtt ggtggcgctt cagccggatc ttgtcgtggg cccaatcgag
     2161 tttctaaagc cggatctggt cttgaaactg gaggagctga aaattcctgt cttcgccctt
     2221 actgaaaaga acgtcgaaga cattttcatc cacgtccaaa cgctgggccg catgttggat
     2281 cgatcgacga ccgcgaatgc tgtggccatg gagctccggc aacaggtcgc acagattaaa
     2341 cagcgtacgg agagattctc tcccgtccgt gttctctatg tgctgaacag tgagcccctg
     2401 attaccgtcg gtcccggcag tttcatcgat caactgattg tcttagccgg tggtgccaat
     2461 gtggcggcga aaagtggaac gccctatcct cggctgagca tggagactgt gctgcacgac
     2521 gatcctgagg tgctggtgtt cccggttgga gagagtgaag gcatctcgga cagtgaacag
     2581 caatcgtggc gccaatggtc gacaatcacg gcggtgaagc aaggacggct ccatcagatt
     2641 cccgctgatc tgctcaatcg accaggcccg cgcgttgtgc gtgcactcga actgttggcc
     2701 gacattcttc atccgaaacg cacctcatcg agtcgcgaga atcagtagcg tgaatcctgt
     2761 gacaccggtg ccaccacctc cggcacacgt cgaatcggcg tcgattgatc accctgcgtt
     2821 gacaacggat acggcacgcg ccctctcgca gtggatgaag accggatcca ttctgaccct
     2881 ccatcggtgg cttctggtca taggggggct catcgtagca agtgttgttg tccttggggt
     2941 gtgcctcgag tttggtgcga tcccggtcga aatgagaacc gtggtgggag ccatactcgc
     3001 actatttcag agggagcaaa tagatgtgga cgggcttggg acccccggta tcattatggt
     3061 ccagattcgt ttgccaaggg ttctgatggg atttcttgtg ggaggcagcc tcgcaggggt
     3121 tggcgtgacg ctacaagcgc tcctgagaaa cccgctggcc gatccgtatg tcttgggtgt
     3181 ttcgagcgga gccgcattgg gggcgtcgtt ggcaatgctg cttggaattg gaacaagctt
     3241 cgctctcctg cccatcttac cgctctttgg atttcttggt ggcctgcttt ccttagtcct
     3301 gatctatcga ctcgctcaat cacagggccg gcttccagtt catagtttgt tactggcggg
     3361 ggtgattctc aatgccattc tgacggcatt gatcatgttc attacgacga tcatggatcc
     3421 caatcgttcg gctggactca tggcttggtt gatgggttcg ttgacagctc ccagctatcc
     3481 tgcacttgcg gtgttcgtac tctatgtagt ggtggtgttg gccatcctga tgcataaggc
     3541 gcagatgctg aacgtgatga cgcttggcga ggacatcgct cgctctttgg gaatcgagac
     3601 ggaatgggtc aagaagcaac tctttgtgct gacggcgcta ttgaccgggg ctgtggtctc
     3661 ggtcagtgga atgatcggat ttgtcggcat ggtgatcccc cacgttgtcc gcatgctcgt
     3721 cgggtcggac catcgtctat tgttccccgc atctgcgctt gtcggcggga tgttcctcgt
     3781 cgtctcagat acgatcgcca gaacgttgct atcgccatcg gaaattccag ttggaatcgt
     3841 tactgcgttg gccggcggac cgttttttct ctatctgctc ttatgccgaa aagatcggat
     3901 gagttgaagt ggcgaaatca accattgaat tagcgaaatc gtggttgtct gagggaagct
     3961 ccgccctgca agtcgacaat ctcttcttcc gatatcgtac gacgtcagag caagcgcgga
     4021 cctgggctct tgaacaggtg tcgtttcaag tcgcggcggg ggagatcctt ggaattgtcg
     4081 gaccaaacgg gtcgggaaaa tcttccctgt tgaaagtcct cgcccgactg gtttcatcgc
     4141 acagcggaac aatcctactg ggagggcaat caatccaaca gttttcgcaa cacgatatcg
     4201 cacggatcgt ggcggtggtt ccccaagaat acgtgcaggt gtttccgttc acggtggctg
     4261 agacagtgtt gatgggacga ttccctcacc ggacaccacg tttctggagc atcggatttg
     4321 gggatgatac taagaaagac ctcgtctgtg ctcaccaggc catgattgaa acggacgtat
     4381 tattgttggc caatcgtctt gtgtcagacc tgtcaggcgg agagcggcaa cgggtggtga
     4441 ttgccagagc cttggctcaa gagccgaaga tccttctcct ggatgaaccc accgcctttc
     4501 tcgatatcaa tcatcagatc gaaatctgtt cgttgatcag tcggcttcgg gcggaacggc
     4561 gattaaccgt ggtgctggtt tcccatgacc tgaacgttgc gagtcagtat tgcgaccgtg
     4621 tgttgatgct gaaggaagga aggctgtatc ggatcggttc gccagccgaa accatacggc
     4681 ccgatgtgct tcggatggtc tatgggtgtg aagtggtcgt ggacgcccat ccgcagacgg
     4741 ggaggccacg tgtgacgatg cccgttgatg ctggttcagc agaaaagtga cgcacgggtc
     4801 atgtccctgg cccggctgtt catccgacgc agggacgatg ccatattcat agaagaaagg
     4861 gcgttttgga gaaacaacgc tggtgacggg gaagatggtg caaatccatc acggtgccgc
     4921 cactgtaagc ggggagtaac tctgcttcat gccactgtgc gcttcggcgc atgggaaggt
     4981 gcagaggaac agaaatccgc gagtcaggag acccagccgg ctgttctttc gactcaaccc
     5041 ttcgagcaca agggaggtag tcatgccgtt tctagcagtg tggtggtttt cgtgcctgtc
     5101 ccttctgggt ttgcttcttc ctgccgacga ggtctttgct caagaggtag cttttgccga
     5161 tcaagaagag attgtcgaaa ctccagaggt cgttgtgacc gcaaccaaga ctgaaactcc
     5221 agtcaagcaa gtcaccagcg ccgtagagat tattaccggc gatcagatga agcagcggaa
     5281 tgtgaagaca gtcgccgagg ctcttcgctg ggcgcaagga cttgcggtct ttcaaagcgg
     5341 agggcctggc acgattgtcg acgtgcgggt gcgaggagga acgccggaac agacgctcgt
     5401 gttgatcgat ggggcgatcg tgaatagtgc cacagtgggc acctatgatt ttgcgaattt
     5461 gacgactgat aacattgagc gaatcgaaat tttgcgtgga agccaaagca tgctgtgggg
     5521 gtccgacgcg atgggtggtg tgatcaacat cacaacgaag cgaggacgtg atataccgag
     5581 catctccgcg ttttccgagt acggatcttt caacaccatt cgagaaggtg ccaacctgaa
     5641 cggcaaaaaa ggaccggtgg acttctctgg ctcaatctca cgctgggata ccgcgagctt
     5701 ttcgtcggtc aattatcgac gcggagcaac tgagcgggat ggataccaca actggcaagg
     5761 atctgttcgc cttggagccg agctccccaa agatgggaga ttagagttta gctttcgttg
     5821 g
//
LOCUS       DATDNW010000298         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_175661, whole genome shotgun sequence.
ACCESSION   DATDNW010000298 DATDNW010000000
VERSION     DATDNW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175661"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..886
                     /gene="hisH"
                     /locus_tag="VI359_07150"
     CDS             254..886
                     /gene="hisH"
                     /locus_tag="VI359_07150"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY6974064.1"
                     /translation="MSVAMIAIIDYGMGNLRSVSKAFEAVGHQAVVTRDVTTIKNASH
                     VVLPGVGAFGDCMANLERYGLIEPIRSTIQSGKPFLGICLGLQLLFTESEEFGLHKGL
                     GIFPGKVRRFMVDKHEGRGLKIPHMGWNQANFQRDCPLFDGIADGANWYFVHSYFVDP
                     SDRQIAATTTTYGIPFVSSIWRDNVLGCQFHPEKSQTVGLQLIKNFGSWK"
     gene            877..1620
                     /locus_tag="VI359_07155"
     CDS             877..1620
                     /locus_tag="VI359_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974065.1"
                     /translation="MEVSLVSRRRWPTAVVGLAVLAAIGCSGQKITTKSSSELTRHPV
                     RTLVLVPFTTLTTPQVRELRDPFFATPQSVRRSDISVAVPQNVEPQDRQTVTVPDYAA
                     EKITQLFWTRLRIREGVTVLPPSESAKASSGNVDVSKSAAETVGAAIAARLKVDAALV
                     GRVLVFQERVGSRLGADPPAAVGFEVKVVASDETVLWIGNYYERQRPMTEDMLGFLQR
                     WGAFVTAEELAQYGVDEILKEFPFGSPGK"
     gene            1644..>2290
                     /gene="hisA"
                     /locus_tag="VI359_07160"
     CDS             1644..>2290
                     /gene="hisA"
                     /locus_tag="VI359_07160"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY6974066.1"
                     /translation="MIIIPAIDLKDGRCVRLRQGDMAAETIYSNDVPAVARRWQEQGA
                     RLIHVVDLNGAVDGEPRNLSEIQSVVKAVSVKVQVGGGIRNIDTVRRYLAAGASRVVL
                     GTAALTDRMFLEQACKEFPRLILLGLDARDGKVAVKGWTAVSETKAIDLLKELSGYEL
                     GAVIYTDIARDGMLGGPNIHALREVVERSSFPVIASGGITRLDDLRAIRSLGPRV"
BASE COUNT          493 a          543 c          738 g          516 t
ORIGIN      
        1 ggtgctgtca accaagggaa tcttataatc gagctcagag ttgagaaagc cggaaggcaa
       61 agggggacag gctagaagag aaacctgtcc ccctttttat ttgcacggtt gggagtagcg
      121 ggacagttcg agttggagta ttgttagaat cctttggacg acgagggaca agcggatcgg
      181 ggagttggca aatcagaggg atgggaatct tatccaacca ccactctaga cctctcgact
      241 ccggagcgtg gctatgagcg tggctatgat tgcgattatc gactacggca tgggcaactt
      301 gcgaagtgtc tccaaggcct tcgaggcagt gggtcatcaa gcggtggtga cacgtgacgt
      361 cactacgatc aaaaatgcga gtcatgtggt actgcctggg gtcggagcct ttggcgactg
      421 tatggcgaat ctggagcggt atggattgat cgagccgatc agatcgacca ttcaatcagg
      481 gaagcccttt ctgggaattt gtctcggact acaattattg tttacggaaa gcgaagagtt
      541 cggtctccat aaggggctcg ggatttttcc tggaaaggtg agacggttta tggtcgacaa
      601 gcacgaaggc agaggtctaa aaatcccaca tatgggctgg aatcaggcta atttccagcg
      661 ggactgtccg ttgttcgacg ggattgcgga tggcgccaat tggtatttcg tacattctta
      721 tttcgtagat cccagtgatc ggcagatcgc tgcgacaacg acgacctacg gcattccgtt
      781 tgtgtcgagc atctggcggg acaatgtgtt gggttgccaa ttccatccgg aaaaaagtca
      841 gaccgtcgga ttgcagctca tcaagaattt tggatcatgg aagtaagtct ggtgagccga
      901 agacgatggc cgacagcagt ggtcggactg gctgtgttgg cagcgattgg ttgcagcggc
      961 cagaaaatca ccacgaagtc gtcatcggaa ttgactcggc atccggttcg cactttggtg
     1021 ctggtgccct tcacgacatt gaccacgccg caagtacggg agctccgcga tccgttcttc
     1081 gcgacgccgc agagcgtgcg ccggtctgat atctctgtgg cggtaccgca aaacgtcgag
     1141 ccacaggaca ggcaaacggt cactgtgcct gattatgcgg ctgagaagat cacgcaactt
     1201 ttctggacca ggctgcggat aagagaaggg gtcacggtgt tgcccccgag cgagtcggcg
     1261 aaagcatcgt ccggcaacgt ggatgtgagc aagtcggcgg ctgagacggt cggagccgcc
     1321 attgctgcgc gactcaaagt agatgccgcg ttggttggcc gggtgctcgt gtttcaagag
     1381 cgcgtgggca gccgcctggg agccgatcct ccagccgctg tgggatttga agtgaaagtc
     1441 gtcgcgtccg atgagacggt cctctggatc ggcaattact atgaacgcca gcggcctatg
     1501 actgaagata tgttggggtt tctccagcgg tggggtgcct tcgtcacggc agaggagctt
     1561 gctcaatatg gtgtcgacga aatcttgaag gaattcccgt tcggttctcc cggcaagtaa
     1621 tctgatcgaa actggttccg ggcatgatca tcattccagc aattgattta aaagatgggc
     1681 gttgtgtgcg actccgtcag ggcgatatgg cagcggagac gatctattcc aatgatgtgc
     1741 ctgctgtggc ccgcaggtgg caggagcagg gggcacggtt gatccatgtc gtcgatctga
     1801 acggtgccgt ggacggcgag ccgcgcaatc tttctgagat tcagtccgtc gtcaaggctg
     1861 taagtgtaaa ggtgcaggtg ggtgggggca tcagaaatat cgatacggtc cgtcggtacc
     1921 tcgccgcggg ggcctcgcgt gtggtgctgg gcactgcagc gttgacggat cggatgttct
     1981 tggaacaagc atgcaaggaa ttccctcgcc tcattctgct tgggctcgat gcccgtgatg
     2041 ggaaagtggc ggtcaagggt tggacggcgg tatcggaaac caaggcgatc gacttgttaa
     2101 aagaattgtc aggctatgag ttgggcgcgg tcatctatac cgacatcgca cgtgatggca
     2161 tgctgggcgg gccgaacatc cacgcgttgc gcgaagtcgt ggagcgatcg tccttccccg
     2221 tcatagcgtc gggtggcatt acgcgcctgg acgacctgcg cgcaattcgg tcgcttgggc
     2281 cccgggtcga
//
LOCUS       DATDNW010000299         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_927418, whole genome shotgun sequence.
ACCESSION   DATDNW010000299 DATDNW010000000
VERSION     DATDNW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927418"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VI359_07165"
     CDS             <1..1212
                     /locus_tag="VI359_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6974067.1"
                     /translation="FQRGIELLETQEASVQGQRLLHDVKRLQQERLTMFYTELERGVA
                     ASGAPSSGYEARRDALTDRMTATLQNYIDLHEASLSVGVSRSRESSAQAEAVTEQLVL
                     VALVFGIGLAGIASYTILRPLRQLQGHIQQIGQGNFRASLQIKAPSELRNLVDTVNWM
                     GQKLQELDDMKAEFLAHVSHELRTPMASIQEGTHLLLDEIPGPLVQEQRTTLRIMADS
                     SRRLIHLISTILDLSKMEAGMMEYRIVPTDLKRITDISVNKVRLLADAKHVQLLVEAS
                     PQRAWVKADALRIEQVLDNLLSNALKFSSEGGIVKVQMKPDSQAGVLEVSVSDSGPGI
                     APEDLPHIFERFYQGRTKTKHSTAGSGLGLALAKKVVEAHGGRIWIESELKKGTTVRF
                     ILRLTKPGVPS"
     gene            1209..2006
                     /locus_tag="VI359_07170"
     CDS             1209..2006
                     /locus_tag="VI359_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974068.1"
                     /translation="MSMARLGAITQTIPMCILWGCASWSTPPPTSHPYFAVEPVDMKA
                     LHALAKKQDALASKCTEHNTCDHVYFTRALLGLYESREAAAKYFDKVIAVAPKSQLAS
                     SSRLWLQLLQEPAPTDRSWFSSVMTAPAISDAHLALGQVSDRLVRDLLDRELMIQQLK
                     ALKDADSQSVEALQRDLAERDRKVEALTNKREVPKVGSETAVVQALQKQVSERDKKIE
                     ELTSQLEALKRIDQEMRDKIRPIRPPSTVVPPSTPAPEPQPPAPTPQ"
     gene            2015..3433
                     /locus_tag="VI359_07175"
     CDS             2015..3433
                     /locus_tag="VI359_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY6974069.1"
                     /translation="MEQEHILVIDDEEGLLQLVKMRLSAMGFTVTACTTGRDAVDAAK
                     KDRFDLAITDLRLRGEDGLDVAEELLRIHPGLPVIILTAHGSIPNAVEAVQRGAFGYL
                     TKPFDDKELKTKIEEGLSQQRMSREIQRLKSLVKELYGMESVVARSPSMQRLLQQVVQ
                     VADSDATILLFGETGTGKEVLARVLHSNSRRSKGPFVALNCAAIPETLFESELFGHVR
                     GAFTSAHGPKRGLFQSANGGTLFLDEIGEMPLSMQVKLLRAVQEREVREVGAETAMKV
                     DVRIIAATNKDLGEAVKRGTFRNDLYYRISVVPLFIPPLRDRRDDIPLLAQLFLTSSG
                     KRANKDMRGFTPAALQRLVTNPWPGNVRELENAVEKAVVMCRQDMITPDLLPSVGFAS
                     ESPLKPLTEAKEEFEKTYLKNVLQLTGGNISRAAQFAGRYRADFYKMLKKYGLHPSSG
                     KGKAEPGIEEIEDVTDLTEAER"
     gene            3722..>3947
                     /locus_tag="VI359_07180"
     CDS             3722..>3947
                     /locus_tag="VI359_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974070.1"
                     /translation="MHQAHQYCIFRRGVIGALSLRLWWGIALALVVGIWTGPEAWALT
                     VSPVSMSFQGVQGATNPPSQSITVSKGNKHQ"
BASE COUNT          987 a         1022 c         1108 g          830 t
ORIGIN      
        1 tttcaacgag gaatagagtt gctggagaca caggaagcat cagttcaagg ccagcggcta
       61 cttcacgatg taaagcggct tcagcaagaa cgtttgacga tgttctacac cgaattggag
      121 cgcggtgtag cggcttccgg cgcaccatca tcgggatacg aagctcgtcg tgatgcgctg
      181 acagatcgca tgaccgcgac gcttcaaaat tatatcgatc tgcacgaggc cagcctgagt
      241 gtcggggtca gtcgatcgcg ggaaagttcg gcacaagctg aagcggtgac ggaacagcta
      301 gttctcgtgg ccttagtatt cggaatcggg ctagctggaa tcgccagtta caccatcctt
      361 cgcccgttac gtcaattgca aggtcacatc caacaaatcg gacaaggcaa ttttcgggcg
      421 tcgctccaaa ttaaggcccc cagtgaactc cgtaatttgg tcgatacagt gaattggatg
      481 ggtcaaaaac tgcaggaact ggatgacatg aaggccgagt tcttggccca cgtctcccac
      541 gaattacgca cgccaatggc ttcgattcag gaggggaccc acctacttct ggacgagatt
      601 cccggtcccc tcgttcagga acagcgcacg acattacgaa tcatggctga tagcagccgc
      661 cgtttgattc atttgatctc gacgattttg gacctctcga aaatggaagc gggcatgatg
      721 gagtaccgca tcgtgccaac agacttgaaa cgtatcacgg atatctccgt gaataaagtt
      781 cgcctcttgg ccgatgcgaa acacgtgcaa ctgttggttg aagcttctcc gcaacgggcg
      841 tgggtcaagg ctgatgcgct tcggatcgaa caagtccttg ataatctcct gtcgaacgcg
      901 ttgaaattca gttcggaagg cggcattgtc aaagtgcaga tgaagccgga ctctcaagcg
      961 ggtgtcctcg aagtatccgt ttcagacagt ggtcctggga tcgcgccgga agatctgcca
     1021 catatattcg aacggtttta tcaaggacgc acgaagacca agcactcgac ggctggcagc
     1081 ggactggggc tggccttggc caaaaaagta gtggaagctc acgggggacg gatttggatc
     1141 gagagtgaac tgaagaaagg aacgactgtc cggtttatcc tacggctgac gaagccggga
     1201 gtcccatcat gagcatggca cgcctcggtg cgatcaccca gacgattccc atgtgtatcc
     1261 tctggggctg tgcgtcatgg tcgactcctc cgccgacctc gcatccgtac ttcgcggtgg
     1321 agcctgtcga catgaaggcg cttcatgcac tggcgaagaa acaagacgcc ttggcgtcca
     1381 aatgtacaga acataacacc tgcgatcacg tgtatttcac gcgagctcta ttagggttgt
     1441 atgagagtcg tgaagcagcg gcgaagtatt tcgataaggt aatcgcggtc gcaccaaaga
     1501 gccaactcgc ctcgtccagc cgattgtggc tccaattgtt gcaggagccg gcaccaacag
     1561 accgctcgtg gttttcgtcg gtgatgaccg cccctgccat ctccgatgcg cacttggccc
     1621 ttggccaagt gtcggatcga ctggtgcggg acttgctaga ccgtgagctt atgatccaac
     1681 agttgaaagc cctgaaagat gctgactctc agagcgtaga ggcactacaa cgggacctcg
     1741 ccgagcgtga tcgaaaagtg gaagcgctca cgaacaaacg ggaagtgcca aaagtcgggt
     1801 cggagacggc ggtggtccag gcgctccaga aacaagtgag tgaacgcgat aagaaaatcg
     1861 aagaacttac aagccagctc gaagctttga agaggatcga ccaggagatg cgcgacaaga
     1921 tcaggcccat ccgaccaccg tcaacggtcg tgccgccgtc tactcctgca ccggaaccac
     1981 aaccaccagc accaacacca caataaggaa gctcatggaa caagaacaca ttctcgttat
     2041 cgacgacgaa gagggtttgt tacagttagt caaaatgcgc ctctcggcta tgggctttac
     2101 cgtgacggcc tgtacgacag gccgtgatgc cgttgatgcg gcgaagaaag atcgcttcga
     2161 tttggcgatt acggatctcc gcctcagagg ggaggacgga ctcgatgtgg cggaagaact
     2221 gctgcggatc catcccggcc ttccggtcat catcctcacg gcacacggaa gcattcccaa
     2281 tgcagtagag gcagtgcagc gcggcgcatt cggctacctg accaagccgt ttgacgacaa
     2341 agagctcaaa acaaaaattg aagaaggact ttcccaacaa cgaatgagcc gggaaattca
     2401 acggctgaag tcccttgtca aagaattgta cgggatggaa agtgtcgtgg cccgtagtcc
     2461 atcgatgcaa cgtttattgc aacaggttgt tcaagtcgcc gattccgatg cgaccatcct
     2521 gttattcggg gaaacgggta cgggtaaaga agtgctggca cgcgtgcttc actccaacag
     2581 ccgtcggagc aagggtcctt ttgttgcgct taactgtgca gcgattcctg aaactctttt
     2641 cgaaagtgaa ctcttcgggc atgtgcgagg ggcatttacc agcgctcacg ggcccaagcg
     2701 cggattattt caaagtgcga atgggggcac gcttttcctg gacgagatcg gagaaatgcc
     2761 actttccatg caagtcaaac tcctccgcgc ggtgcaagaa cgggaagtgc gtgaggtagg
     2821 ggccgaaacg gcgatgaagg tggatgtccg gatcatcgcc gcgaccaaca aggatctcgg
     2881 cgaagcagtg aaacgcggga cattccgaaa cgacctctat tacaggatat ccgttgtgcc
     2941 attgttcatt cctccccttc gcgatcgacg agacgacatt ccgcttctcg cacaactgtt
     3001 tttaacctcc agtgggaagc gcgccaacaa ggatatgagg ggatttaccc cggcggcgct
     3061 ccagcgtctc gtgacaaatc catggccagg taacgtccgt gaacttgaga atgcagtgga
     3121 gaaagcggtg gtgatgtgtc gacaagacat gatcacgcca gacctccttc cgtcagtggg
     3181 atttgcgtca gagtctccgc tcaaaccgtt aacggaagcc aaagaggagt ttgaaaagac
     3241 gtatctcaag aacgtgttgc aactgacggg cggcaacatt tcgcgagccg ctcaatttgc
     3301 gggacgctat cgggcggatt tttacaagat gctgaagaag tatgggctcc atccctcgtc
     3361 gggaaaagga aaagcagagc ctgggatcga ggagatcgaa gacgtaaccg acttgaccga
     3421 agctgaacgg tgaagtgcgc gaggtatcca gtcgcccagc acattaagag gaatgttctc
     3481 tgaacaatgt ttgccgtccg tgaattgtta agcctacaca ttgggcaaga cttctgccga
     3541 tcgactgtgt gcgcaaacca cctgtaattc atatccagtc aacaacttcc gcctgggcct
     3601 cctgatggca tcgttgttgc tcgtcagaaa aagcgatgtc gaatagcatg gaggaacggc
     3661 cagtgcccgt ttctcaccaa tgtgtgcgag aaacaactag tcacacgcca ttgaggttta
     3721 tatgcatcaa gcccatcaat attgtatttt ccgacgaggt gtgatcggtg ccctctcact
     3781 ccgactctgg tggggcattg ctctggcatt ggtggtcggc atctggacag gccctgaggc
     3841 gtgggcacta actgtgagtc cagtgagcat gagttttcaa ggcgttcagg gtgcgaccaa
     3901 cccacctagt cagtcgatca ccgtctccaa aggaaataag catcaga
//
LOCUS       DATDNW010000300         4182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_661380, whole genome shotgun sequence.
ACCESSION   DATDNW010000300 DATDNW010000000
VERSION     DATDNW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661380"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..802)
                     /locus_tag="VI359_07185"
     CDS             complement(2..802)
                     /locus_tag="VI359_07185"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEY6974071.1"
                     /translation="MPKPKPAPLRERDITRQIAREYYKEFDQMIESDVIIVGAGPSGL
                     ICAHDLADMGFRTVVVEQSLALGGGFWHGGYLMNKATICAPAHKILEEIGVPCKKVKD
                     CDGMYIVDPPHATGALVAAAYRGGAKIMNLTRVVDLILRRDGVLEGVVVNNTTAEMAG
                     HDIIHVDPIALESKIVVDATGHDAVVVNLLHKRNLYTPVPGNGAMWVSRSEEDVMDHT
                     GEVYPNCFVVGLAVAAVYGTPRMGPAFGSMLLSGRYGAELIKKKLKNE"
     gene            complement(813..2714)
                     /gene="thiC"
                     /locus_tag="VI359_07190"
     CDS             complement(813..2714)
                     /gene="thiC"
                     /locus_tag="VI359_07190"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY6974072.1"
                     /translation="MSEHVPTSGNGKKTASVTLTTDPFPASRKVYINGSQPGVHVPMR
                     EISLTPTKSMNGGTPTPNEPVTIYDTSGPYTDPSVTIDVRTGLAPLRRPWVVSRQDVE
                     QLPDVSSQYGRQRAADPKLADLRFQHIRKPLRAKPGQNVSQLHYARKGIVTPEMEFIA
                     IRENQSREVARELAHRNGNGGGVAQHPGQSWGASIPSTITPEFVRDEVARGRAIIPAN
                     INHPESEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMIWSIRWGADTVMDLSTGKN
                     IHETREWIIRNAPVPIGTVPIYQALEKVGGKAEELTWEIFRDTLIEQAEQGVDYFTIH
                     AGVRLAYVPLTANRMTGIVSRGGSIHAKWCLAHHEENFAYTHFEDICEIMKAYDVSFS
                     LGDGLRPGSIADANDEAQFAELETLGELTKMAWKHDVQVMIEGPGHVPMHMIHVNMEK
                     QLKACHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPDR
                     EDVKAGVIAYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPDTAKQF
                     HDETLPDNAAKVSHFCSMCGPHFCSMKITQDVRDYAASKQVSEQQAIQIGMKEKAEEF
                     KKTGAEIYR"
     regulatory      complement(2806..2960)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3128..>4182
                     /gene="nadA"
                     /locus_tag="VI359_07195"
     CDS             3128..>4182
                     /gene="nadA"
                     /locus_tag="VI359_07195"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY6974073.1"
                     /translation="MNAISTLPRPITDYQQLSAEELFARTAEAKRSLGDQVMILGHNY
                     QRDEVIEHADFRGDSLLLSKLAAERSDRPYVVFCGVHFMAETADILSRTNQTVILPDM
                     AAGCSMADMAAIEQVDQCWDSLGRVLPVEETVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNAKAVIEWSWARRDKILFFPDEHLGRNTANKMGVPREQMIVWDPYQPNGGNTREAIQ
                     RAKLILWKGHCSVHQMFQPVHVDNFRRQYPDGKVIVHPECHEDVVNKADLVGSTEFII
                     KTVSAAPAGTTWAVGTELNLVNRLKRDLTNKKVFFLSSTVCQCATMFRIDGAHLCWAM
                     ENLAEGHVVNQIVVPDD"
BASE COUNT          825 a         1168 c         1225 g          964 t
ORIGIN      
        1 gttattcgtt cttcagcttc ttcttgatca gctcggcacc ataacgaccc gacagcaaca
       61 tcgagccaaa ggccgggccc atacgcggtg tcccatagac cgcagcgacg gccaggccca
      121 cgacgaagca attgggatag acctcgccgg tgtgatccat gacgtcttcc tccgagcgtg
      181 agacccacat tgcaccgtta ccagggaccg gtgtgtagag attgcgcttg tgcaacaagt
      241 tcaccacgac ggcgtcgtgg ccagtcgcat ccacgacgat tttgctctca agcgcgatgg
      301 gatcgacatg gatgatatca tgacccgcca tctcggccgt cgtattgttg acgaccacac
      361 cttccagtac gccgtctctt cgtaggatca agtcgaccac cctggtcaga ttcatgatct
      421 tggctccacc ccgataagcc gccgcaacga gcgcaccggt cgcatggggc ggatcgacga
      481 tatacatgcc gtcgcagtct ttgaccttct tgcagggcac gccgatttct tcgaggatct
      541 tgtgtgccgg ggcgcagatg gtggccttgt tcatcaaata gccgccgtgc cagaacccgc
      601 cacccaaggc gaggctctgt tccacgacca ccgtgcggaa gcccatgtcg gccagatcat
      661 gcgcacagat cagaccggac ggcccggcgc cgacaatgat gacatcgctt tctatcatct
      721 ggtcgaattc tttgtagtat tcgcgcgcga tctgcctggt aatatctctt tcacgaaggg
      781 gtgctggttt gggcttgggc atggtcgtgc tcctaccggt aaatttccgc tccggtcttc
      841 ttgaattctt ccgctttttc cttcatgccg atttgaatgg cctgctgttc gctcacctgc
      901 ttgctcgcgg catagtcacg cacgtcttgc gtaatcttca tcgagcaaaa gtgcggtccg
      961 cacatcgaac agaagtggga gactttcgcg gcattgtccg gcaaggtctc atcatggaat
     1021 tgtttggccg tgtcaggatc gagtgacaga ttgaactggt cttcccagcg gaattcgaag
     1081 cgggccttcg acagcgcgtt gtcgcgaatc tgtgcgcccg gatgtccctt ggctaagtca
     1141 gcggcgtgtg ccgcgatctt gtacgcgatc acgccggcct tcacgtcctc gcggtcgggt
     1201 aagcccagat gttcttttgg tgtgacgtaa cagagcatgg cacagccgta ccagccgatc
     1261 atcgctgcac cgattcccga cgtaatatgg tcgtaaccgg gcgcaatatc ggtggtgagc
     1321 gggccgagtg tgtagaacgg cgcttcgtgg catgccttga gctgcttctc catattgacg
     1381 tgaatcatgt gcatcggaac gtgaccgggg ccttcaatca tcacctgcac atcgtgcttc
     1441 caggccatct tggtcagttc tcccagtgtc tccaattcag cgaactgggc ctcatcgttg
     1501 gcgtctgcaa tcgaaccggg tcgcaatccg tcaccgaggc tgaacgaaac gtcataggcc
     1561 ttcatgatct cgcagatgtc ttcaaagtgg gtataggcga agttttcttc gtgatgagcc
     1621 aggcaccact tggcgtggat cgatccgccg cgagacacaa tgccggtcat gcggttggcg
     1681 gtcagaggca cataggcgag gcgcacgcct gcatggatcg taaagtaatc gacaccctgt
     1741 tcggcctgtt cgatcaaggt gtctcggaag atctcccaag ttagctcttc ggccttgccg
     1801 ccgacctttt cgagcgcctg atagatggga accgtgccga tgggtactgg cgcgttgcga
     1861 atgatccatt cgcgcgtctc atgaatgttc ttgccagtcg agaggtccat taccgtatcg
     1921 gcaccccagc ggatggacca gatcatcttc tcgacttctt cctcgattga ggaggccacg
     1981 gcagaattgc cgatgttggc gttgatcttc accagaaaat ttcggccgat gatcatcggc
     2041 tcgctttccg ggtgattgat gttggctggg ataatggcgc ggccacgcgc gacttcatca
     2101 cgaacaaatt ccggagtgat ggtggacggg atactcgcgc cccaggattg gccggggtgc
     2161 tgggcgaccc cgccgccgtt gccgttccga tgggccagtt cgcgcgcgac ttcgcgtgat
     2221 tggttttcgc ggatagcaat gaactccatt tccggggtga cgatgccctt gcgggcgtag
     2281 tgcagctgcg agacattctg cccgggtttc gcacgaagcg gcttgcgaat atgttgaaag
     2341 cgaaggtccg ccagtttggg atctgccgcg cgctggcggc catattgtga cgagacatcc
     2401 ggcagctgtt cgacatcctg ccgactgact acccacgggc ggcggagcgg tgccagacca
     2461 gtgcgcacgt cgatcgtcac cgagggatcg gtatagggac ctgacgtatc gtaaatggtg
     2521 accggttcgt tcggggttgg cgtgcctccg ttcatggatt tggtcggtgt caggctgatt
     2581 tcccgcatgg gcacatggac accgggctgt gatccattga tatagacctt ccgcgacgcg
     2641 ggaaagggat cggtcgttaa cgtgacagat gcggtcttct tcccgttgcc acttgtgggt
     2701 acatgctcac tcatggggaa tcctttctca atatcgtcat ccgcgaagcg gctcaaataa
     2761 aaaagccgca cctcaataga gagggtgcgg ctgagaggaa cgtcgttctc gttgttcccg
     2821 cttccctacg ctggtattac ccaggtcagg tttcaaggac cgcgccggat tcgcctcatc
     2881 acaggcgact tctccagggg ggtcctaagg gtcatccgat cgctcggact ctcagccgta
     2941 tggcacccct agctatggag aagaatcgta gtctcgggct ggccggttgt caacgggtta
     3001 ttaggcctac agggatcgtg ccggttggac catcaggagc ttggacttcg ttacggcagc
     3061 cgttgcgttg attgtagcag gtgcctatcg tagaatgacc aactagaggg tttcccggga
     3121 ggattatgtg aacgccatat cgacccttcc tagaccgatt acggactatc agcagctttc
     3181 tgccgaggag ctctttgccc ggacggcgga ggcaaaacga tcgctcggcg atcaggtcat
     3241 gatcctcggt cataattacc aacgggatga agtcatcgag catgcggatt ttcgggggga
     3301 ttctctcttg ctctcgaaac tcgctgccga gcggtcggac cggccctatg tcgtcttttg
     3361 cggcgtccac ttcatggcgg aaacagcgga tattctgagc cgcaccaatc aaacggtgat
     3421 tcttccggac atggcagccg gatgctccat ggcggacatg gccgcgattg agcaggtcga
     3481 tcaatgttgg gattcattag ggcgtgttct cccggtcgaa gagacggtca tgccggcggt
     3541 gtacgtgaat tccgctgccg tgctcaaagc attttgtggc gaacatgggg ggatcacctg
     3601 cacgtcgtcg aacgccaagg ctgtgatcga atggtcctgg gcccgccgag acaagatttt
     3661 attcttcccc gatgagcacc tcggtcgcaa taccgccaat aagatggggg tcccgcggga
     3721 gcagatgatt gtctgggatc cctatcagcc gaatgggggg aatacgcgcg aggcgatcca
     3781 gcgcgccaag ctcattctgt ggaagggcca ttgcagcgtg catcagatgt tccagccggt
     3841 gcacgtggac aatttccgca ggcagtaccc ggatggaaag gtgatcgtcc atccggaatg
     3901 tcacgaagac gtggtcaata aagctgactt ggtgggctcg acggagttca tcatcaagac
     3961 cgtgtctgcc gctccggccg gaacaacgtg ggcagtcgga accgaactca atctcgtcaa
     4021 ccgtctgaag cgtgatttaa ccaacaagaa agtgttcttt ctgtcctcga ccgtctgcca
     4081 atgcgccacg atgtttcgta tcgatggagc tcatctctgc tgggcgatgg agaatctcgc
     4141 cgaaggccac gtcgtgaacc agatcgtcgt tccggacgat ga
//
LOCUS       DATDNW010000301         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_106339, whole genome shotgun sequence.
ACCESSION   DATDNW010000301 DATDNW010000000
VERSION     DATDNW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106339"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VI359_07200"
     CDS             complement(<1..126)
                     /locus_tag="VI359_07200"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY6974074.1"
                     /translation="MEAVRTIEVAAGLINRDGRYLIARRKSGVHLAGLWEFPGGKR"
     gene            complement(127..855)
                     /locus_tag="VI359_07205"
     CDS             complement(127..855)
                     /locus_tag="VI359_07205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY6974075.1"
                     /translation="MPRKTQPRTVTPQKDRKPSHPSRLDASLKRRFQQKLLKWYKEHG
                     RDLPWRKTSDPYHILVSEVMLQQTQVDRVIPKYHEFLDRYPSFEDLAEAPVGDVRKTW
                     FPLGYNIRPERLHGIACETVARYGGKLPSGHKELMSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVMRVLHRVFVGEGTPKSQKRKLWELSGALIPRGRGYDFNQAMMDFGAMCCTAR
                     NPYCLLCPMKAFCKSYPWDSGQED"
     gene            1034..1351
                     /locus_tag="VI359_07210"
     CDS             1034..1351
                     /locus_tag="VI359_07210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974076.1"
                     /translation="MVVRKYPRFPVSFSSTLVQRDAFRHKGSLRDLSLRGCRVQTVIQ
                     PFTGMQVELHLHIPGDPTPITITNATVRWSGSHGVGMEFLNVAPPQQERLTRVIQQLA
                     KKA"
     gene            complement(1355..1570)
                     /locus_tag="VI359_07215"
     CDS             complement(1355..1570)
                     /locus_tag="VI359_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974077.1"
                     /translation="MTRKPGEPIYLRIHMVALVLAVVASIAIPHLYELFFGPISLEAR
                     LIAGLIIAVLAGGVLYRTYQSSARNEP"
     gene            complement(1584..>2009)
                     /locus_tag="VI359_07220"
     CDS             complement(1584..>2009)
                     /locus_tag="VI359_07220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6974078.1"
                     /translation="LAETSTAAFDEVMNVNLRGTFFCCRAGFKQMKKQNGGLIINMSS
                     VAGVQAWAGTGTYSASKHAVMALTKSLADEGRHFKIKVSAICPSGVADELVDASPEEI
                     LRSEKISPWDIADTAIFLATLGPYAVVQQVVVDRLGADW"
BASE COUNT          450 a          600 c          507 g          452 t
ORIGIN      
        1 acgttttcca ccaggaaatt cccagagtcc tgccaaatga acgcccgact tgcgccgggc
       61 gatcaagtaa cggccatcgc ggttgatcag acccgcggcg acttcgatcg tccgcacggc
      121 ttccattcaa tcctcctgac ccgagtccca gggataggat ttacaaaatg ctttcatggg
      181 gcaaagcaga caatagggat tcctcgccgt gcaacacatg gctccaaagt ccatcatcgc
      241 ctgattaaag tcgtatcccc tgccgcgcgg aatcaacgcc ccggataatt cccacagctt
      301 tctcttctgc gatttggggg ttccctcacc cacaaaaacg cgatgcagca cgcgcataac
      361 gttcgtatcg agaatcggtg cgtcttcatt gaaggcaaac gaccgaatgg cacccgccgt
      421 atagcgacca attcccttga acgacatcaa ctccttatga ccactcggaa gttttccccc
      481 ataccgagcg actgtctcac aggcgatgcc gtgcaatcgc tcgggacgaa tattgtagcc
      541 taacggaaac caggtcttcc ttacatcccc gaccggtgct tccgccaagt cttcaaagct
      601 gggatagcga tcgaggaact catgatactt cggaatcacc cggtccactt gggtctgctg
      661 gagcatcact tcggacacga ggatgtggta ggggtctgat gttttccgcc aaggtaaatc
      721 ccgtccatgt tccttgtacc atttgagaag cttctgctga aatcgccgct tgagcgaggc
      781 atcgagccgg gaggggtgtg atggcttcct atccttctgg ggtgtgaccg tccgaggttg
      841 agtcttgcga ggcatcccga gtcaccgcgt caggcggcat tgtaggaggg acctcgttga
      901 aaagcaacga tgcacggctg ttaggaatct tcgtttttct agccatcgat cgaggcctct
      961 gccacaattc tcctcaatgc tacggactgg tcctcagtcc aatacagcca gagttgatca
     1021 caagagaagc agtatggttg ttcgtaaata tcctcgattt cccgtgtctt tttccagcac
     1081 gctcgtgcag agggatgcct tccggcacaa gggatcgctg cgggatctct ccctgcgagg
     1141 ttgccgcgtt caaacggtta ttcagccctt taccggcatg caagtggagc ttcatcttca
     1201 cattcccgga gatccgactc cgatcacgat cacaaacgcg acggtgcgct ggtccggttc
     1261 acacggtgtc ggcatggagt ttttgaacgt cgccccgccc caacaagaac gtctcacacg
     1321 agtgattcag caacttgcaa agaaggccta ataactatgg ttcgttccgg gcggaggatt
     1381 gataggtccg gtagagcacg ccgcccgcga gaacagcgat gatgagtcca gcgataaggc
     1441 gggcttcgag acttatcgga ccaaagaaca gctcgtagag atgaggaatc gcaatcgacg
     1501 cgacaactgc cagcaccagt gccaccatgt ggatccgtag gtagatcggc tcgcccggtt
     1561 ttctcgtcac gaccttcctc cgctcaccag tccgctccca atcggtccac aaccacctgt
     1621 tggactactg catagggtcc gagggtcgcg aggaagatcg ccgtatccgc gatatcccac
     1681 gggctgatct tctcactccg aaggatctcc tctggcgacg cgtcaacgag ttcatcagca
     1741 accccagatg gacaaatcgc gctgacctta atcttgaaat gacgcccttc atcagccaac
     1801 gacttcgtca gagccatcac ggcatgtttg gaagcgctat aggtcccggt tccggcccag
     1861 gcctgtaccc ccgcgacgct tgacatattg atgatcaatc ccccgttttg ctttttcatc
     1921 tgcttaaatc cggctctgca acagaagaac gttccccgca ggttgacatt catcacctcg
     1981 tcgaaagccg ctgtactcgt ctctgccag
//
LOCUS       DATDNW010000302         7449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_514842, whole genome shotgun sequence.
ACCESSION   DATDNW010000302 DATDNW010000000
VERSION     DATDNW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514842"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VI359_07225"
     CDS             complement(<1..191)
                     /locus_tag="VI359_07225"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974079.1"
                     /translation="MSSTHDQPLPPPTAVNLLALDERGVGQIVQSLGWPKYRTGQVLR
                     WLHRRGVRTIAQMTDLSLT"
     gene            437..2566
                     /locus_tag="VI359_07230"
     CDS             437..2566
                     /locus_tag="VI359_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6974080.1"
                     /translation="MCDAVDQCFQAAALPKERLGKALTKEQVLSLKLERLKRIMERFP
                     ATVWAKRAGLLSGVLLIERNPAVAIQFLRGAQRDFPALDDYVRLWIGEALLNLGDAKQ
                     AAEQFESISREVPDSNILTRAAYRTGEAWYQASRCAEATEWFEKAVRLSDKETSTAQA
                     LLRRAACQLKDNNIADGRETLKQLWVRFPYSAEGREANTLLASNLGGEPWKPQPAERL
                     SRAQAYFGQAYHAEAIEELRKFLAAEPNSPQRSEAKLKLGIAQARLKQYDQARETFQS
                     LLKNGSPESREASVWLARVYLRLGQGDKLLELARFSQNGSLTGEQKGQITLLVGVWLE
                     DQGQFDEAIARYRQVMKSGEPASQRAEGLWRVGWVYYRTARYREAVETLRVIADQHDT
                     DFEPQALYWIARASEQTEPSRARDFYLQVCTRYVYTYYCQLARERSDVPAPPPSSPEV
                     VTADAQSSMAPDTSLPRNNRSEIEQQLAYRRAIELKVLGLELDAAHELAVLTDRYAQD
                     ASTLMALSVMLNEVGAYHHALRLARARFRDKLERTGGPVAPEMWEVAYPTGLVTTIKD
                     QGAKGVDPYLVAAIIREESQYDWKAVSRVGAIGLMQVMPGTANAVAQRYGFPAVTRDD
                     LFDQETNIRIGVRYVEQLLDQFSGNVVQTIAAYNAGPVVVGSWIALHRGRSQDEFVEL
                     IPYQETRQYVKRVLRSYREYIRLNGTS"
     gene            2656..2910
                     /locus_tag="VI359_07235"
     CDS             2656..2910
                     /locus_tag="VI359_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEY6974081.1"
                     /translation="MSLDRLDALESRIRDLVKLVQELKRRNAALEGELKAARERLAAQ
                     NDSNRRWERERVDIKSRVEKVLGELDLLDGYDERKEVALD"
     gene            2903..3199
                     /locus_tag="VI359_07240"
     CDS             2903..3199
                     /locus_tag="VI359_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY6974082.1"
                     /translation="MTKTIDVEIYGQRYAISGDADEGYIRRLAHFIDDQMRRVAEGMN
                     TTTPSRLAVLTALNLAHQMFELEKKRVQGEADVERRMMSLMASIEEQVPTSLFR"
     gene            3772..5349
                     /gene="rny"
                     /locus_tag="VI359_07245"
     CDS             3772..5349
                     /gene="rny"
                     /locus_tag="VI359_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY6974083.1"
                     /translation="MSPISTSIMANLLIGMIGALLGIGLYEMIRRMASAAKQSRAEEQ
                     SRQVAQNAQREAENIIKEAKLEAKDLLLQSKAELEKEQKLKLAELSSNEKRLVQREET
                     IEKRVTTLDRRDSEAQKREQELAKRDERLTAKEFACAQAEKEHREALERVAGMTADEA
                     KKHLLQEMESQARLDAAGIAKRTLEEARENAEREAREIITTSIQRVVRDYVSESTISV
                     VPIPNDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIVSGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIDKLMVEEAEKIIFELGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREASYICGIMASELGLDVKLARRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGESEKIVNAIAAHHEQVPPLCPESVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATAIKGVQKAYAIQAGREIRVIVRQEDLTDSESFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRYVEYAK"
     gene            5346..6125
                     /locus_tag="VI359_07250"
     CDS             5346..6125
                     /locus_tag="VI359_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY6974084.1"
                     /translation="MKVLIIGDIMGEPGRRAIARAMPRLVSQRQIDIVIGNGENIAGG
                     FGITPDLAEELFEMGLAVITTGNHAWDKKEILDYFPREPRLLRPANYPAGVPGYGSYV
                     IETSSGEHLAVLHLMGRAYMPTLDCPFQVARKELVGLKNQAAAVIVDMHAEATSEKMA
                     MGHYLDGEVTAVVGTHTHVQTADEQILPKGTAYLTDIGMTGPLHSVIGVKKELAIEKF
                     LTGMPRRFEVASGPTVFCAALVELDARLGKALSIERIRIID"
     gene            6254..>7449
                     /gene="xseA"
                     /locus_tag="VI359_07255"
     CDS             6254..>7449
                     /gene="xseA"
                     /locus_tag="VI359_07255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY6974085.1"
                     /translation="MWLEGEISNLRTPGSGHLYCTLKDNTSQIRAVLFRSNGLRLRFA
                     LQEGLHVIVRGRLTVYEPRGEYQIVLEHVEPKGIGALQLAFEQLKERLAAEGLFDQKR
                     KKSLPAFPRAVGLVTSIAGAAIHDILTVLQKRWPLLHIIIAPVQVQGEGAAKQIADAV
                     ASLNSIRKIDVIIVGRGGGSWEDLWSFNEEAVVRAIVASRIPVVSAVGHEIDVTLADL
                     AADVRAPTPSAAAEMVVPVLSHLVEQLDDYTVRMQRVMTRRCAFERHRLETYLSQISH
                     MRFYVQERSQLVDELTGRLCHAVDELRSIMKQRVHGRYQDLLACNPLLFVKQGLTLIP
                     QLVTRLHRQMKSVILHRRQRIEQAVAELNNLSPLAILARGYSILQNVGTGRIIRHAKD
                     VRVGDE"
BASE COUNT         1745 a         1820 c         2236 g         1648 t
ORIGIN      
        1 tccgtcaagc tgaggtccgt catctgggcg atggtccgca cccctcgccg atggagccac
       61 cggagcacct gtcccgtgcg gtacttgggc cacccgagac tttggacaat ctgacccaca
      121 ccgcgttcat caagggctaa gaggtttacc gctgtgggcg gagggagcgg ttggtcatgt
      181 gtcgaagaca ttcatgcagc ctagcacagg gtgaaaaagt ggaacaagca cgaagcccca
      241 atcggtttgc tggcaacaaa acgaggacgc tgatataaag agagaagagt cgtaacggct
      301 agagacggtg cacatgtcag tttacgtacc ttccatgcgg atcctcttct cgcttgtgtt
      361 gataactgcg tggggaatgg gtcttcctct cgaccaacag cgtgtagctt ctgcatcaat
      421 acaagaagac acggacatgt gtgacgcggt ggaccagtgt ttccaggcgg ccgcgctgcc
      481 taaagaacgg ctgggcaagg ctctgacgaa agagcaggtc ttatcgctca agctggagcg
      541 cctgaagcga atcatggaac gatttcctgc caccgtctgg gccaagcggg cggggttgtt
      601 gtccggcgta ttactcatcg agcggaaccc tgccgtggcg attcagtttc tccgcggggc
      661 tcaacgcgat tttccggcct tggacgatta tgttcgactc tggatcggtg aggctctgtt
      721 gaaccttggc gatgcgaagc aggcggcgga gcagtttgaa agcatttctc gcgaggttcc
      781 cgattcgaac attctgacca gagcggccta tcgaacaggg gaggcctggt accaggcctc
      841 tcgttgtgcg gaggcaaccg agtggttcga gaaggctgtg cgattgagcg acaaagagac
      901 tagtactgcg caggcgttgc tccgccgcgc agcctgtcag ctcaaagaca acaacatcgc
      961 cgatggaaga gagacactga aacaattgtg ggttcgattt ccatactcgg ctgaaggcag
     1021 ggaggcgaac accttgctgg cttctaatct cgggggcgag ccctggaaac cgcagccggc
     1081 ggagcggctt tcgagagctc aagcgtattt tgggcaagcg taccatgccg aggcgatcga
     1141 ggaattacgg aagtttttgg cagcggaacc gaattctccg caacggtccg aagcgaaact
     1201 gaagctcggc atcgcgcagg cacggctcaa acagtacgat caggcacgag agacctttca
     1261 gagtctctta aagaatggtt caccggagtc tcgtgaagcg tctgtctggc tcgctcgcgt
     1321 ctatttgaga ctcggccaag gggacaaact cttggagctg gcgcggttca gccaaaatgg
     1381 atcattgact ggcgagcaaa agggacaaat tactctgctg gtcggagttt ggttggaaga
     1441 ccaagggcaa tttgatgaag cgatagcgcg ttaccggcaa gtgatgaaat ctggggaacc
     1501 ggcgtcccag cgagctgaag ggctctggcg tgttgggtgg gtttactatc gcacagctcg
     1561 ctatcgtgaa gcagtggaga cgctccgtgt tattgccgat cagcatgata ctgacttcga
     1621 accgcaggcg ctctattgga tcgcacgcgc ctccgagcag acggaaccat ctcgtgcccg
     1681 cgacttctat ctgcaggtgt gcacccgcta tgtctacacg tattattgtc aattagcacg
     1741 agagcggagc gatgttcctg caccgcctcc ttcctcacct gaggtggtca cagccgatgc
     1801 gcaatcgtca atggcgccgg acacgtccct tcctcgaaac aaccgatcag agatcgagca
     1861 gcaattagcc tatcgtcgcg cgatcgaact taaggtcttg ggtttggagt tggacgcggc
     1921 ccatgagttg gccgtcctga ccgatcgcta tgctcaagat gctagcacct tgatggccct
     1981 ttcagtcatg ttgaacgagg ttggcgccta tcaccatgcg ctccggctgg ctcgggctcg
     2041 tttccgagat aagttggaac gtaccggtgg tccggtcgcg ccggagatgt gggaggtggc
     2101 ctacccgacg ggtctggtca caacgattaa ggatcaaggc gcaaaagggg tcgatccgta
     2161 tctggttgcg gcgattattc gagaggaaag ccaatatgat tggaaagcgg tttctcgtgt
     2221 gggcgctatt gggctgatgc aagtgatgcc cggaacggcc aacgccgtgg cccaacgcta
     2281 tggatttccg gccgtgacgc gagacgatct gttcgatcaa gaaacgaaca ttcggattgg
     2341 cgtacgctac gtcgaacagt tactcgatca gttttctggc aatgtggtgc agacgatcgc
     2401 cgcttataat gcgggaccgg tcgtggtggg aagctggatc gccttgcatc gtggtcgaag
     2461 tcaggatgag ttcgtcgagc tgattccgta tcaggagaca cggcaatacg tgaagcgggt
     2521 tctacgaagc taccgcgaat acattcgttt gaacggtacg tcttaaccac ctttcttgac
     2581 aatatctggg caagtttcta tagttccccc caatccgttg ggggtctctg gaggaagaag
     2641 gagagcgcac gggctatgtc tttggatcga ttggatgcac tagagagtcg aatcagagat
     2701 ctcgtgaagt tggtgcagga gttgaagcgt aggaacgccg cacttgaagg agaactgaag
     2761 gccgctcggg aacggcttgc cgcgcaaaac gattccaacc gacgttggga acgggaacgc
     2821 gtcgacatca aatctcgggt cgaaaaggtc ttgggagaac ttgatctgct ggacggctac
     2881 gacgaacgca aggaggtggc ccttgactaa gaccattgat gtggaaattt atggtcaacg
     2941 ctatgccatt agcggtgatg cagacgaagg atatatccgg cgtctggcac acttcatcga
     3001 cgatcaaatg cgacgggtgg cagaaggtat gaatacaacc acaccctcga ggttagcggt
     3061 cttaacggcc ctcaacttag cccatcagat gtttgaattg gaaaagaagc gagtgcaggg
     3121 cgaggcagat gtcgaacgcc ggatgatgtc gctcatggcc tccatcgaag agcaggttcc
     3181 gacctcgctc tttcggtaga ttcatcttgc aaagggctgg aatcgtttgg tattgtaaat
     3241 cacgtgaaaa gcagcggcag gtgctgtttg agacgatagg cgtaagagtg taggtcaact
     3301 tcgaccggtt atgttctccg agaatctgac gtagaaaccg taaggatgtt agtaggatcg
     3361 tcgatgctga cggcgttgag acaggggaaa ccgatatcaa agttagtact tcttgggcgg
     3421 gctttgttcg agcagttcgc agagtggttg gtgtttatgg gacgaagtgt ctgtggaaat
     3481 gatccacggc ctcttgcagt tctcgtggct tctcctttca gtcgatcgag aggagggcga
     3541 cggacacgct tctctatcga ggtgctgaca agacctgcgc ggcgtctccc ttcagcgtcc
     3601 tgtcctccat tcgcgattcc cttctcggag tgagcagagt tctcggttcg acctgacttc
     3661 ttctcttgac gcctcatccc tcttaacgca ccttaccggc tcgatacttt agcaaatgtg
     3721 tgtggtcggg actgagaaag tcgatctatc tcgtcccgag atgagaaggg ggtgagtccc
     3781 atttcgacct cgatcatggc aaatctgctc atagggatga ttggagcgct cctcggaatc
     3841 ggactgtacg agatgattcg tcgaatggcg agcgccgcaa agcaatctcg tgcggaggaa
     3901 cagtctcgtc aagttgcgca gaacgcacag cgagaggcgg aaaatattat caaggaagcc
     3961 aagcttgaag cgaaggatct cctactgcag tcgaaagcgg aacttgagaa ggagcagaaa
     4021 cttaaactgg cggaattgtc gagtaacgaa aagcgtttgg ttcagcgtga agagaccatc
     4081 gaaaaacggg tcactacgtt ggatcgacga gatagtgaag cgcaaaaacg ggagcaggaa
     4141 ctcgcgaagc gcgacgagcg attgacggcc aaagaattcg cctgtgcgca ggcggagaaa
     4201 gaacatcgcg aggcactgga acgggttgcg ggtatgacgg cagatgaagc gaaaaagcat
     4261 ctgctccaag aaatggaaag tcaggctcgg ctcgatgctg cgggtattgc caaacggact
     4321 ctcgaagagg caagggaaaa tgcggagcgg gaggcccggg agatcattac cacctctatt
     4381 cagcgggttg tgcgtgacta cgtctcggag tcaacgattt cagttgtccc gattccgaat
     4441 gatgctatga aaggacggat catcggacgt gagggacgaa atattcgtgc gatcgaagcg
     4501 gcgaccggaa tcgacctgat tatcgacgag actcccgaag cagtgattgt ctctggtttc
     4561 gatccgcttc gtcgggaaat cgctaaggtg tcccttgaac ggttgatgca cgatgggcgg
     4621 atccatccga ctcggatcga agagattgtc gagaaggtta aggtggatat cgacaagctg
     4681 atggtcgaag aggcagaaaa gatcatcttc gagttagggc tttcggactt ccacccggaa
     4741 ctcgttaaag ttctgggtcg gctgaagtac cgcacgagct atgggcaaaa taatctctac
     4801 catgctcggg aagcctccta tatctgcggg atcatggcat cggagctcgg cttggatgtg
     4861 aaattggcca ggcgaggtgc gctattgcac gacattggca aggctgtgag ccacgaggaa
     4921 gaaggaccac atgccatgtt gggtgccgag atcgcgaaga agtatgggga aagcgaaaag
     4981 attgtcaatg cgattgcagc ccaccatgaa caggtgccgc ctctgtgtcc ggagtcggtt
     5041 ttggtggcgg cggcggaggc gttgtcagct gcaagaccgg gtgccagacg ggaggcgttg
     5101 gagtcttacg tcaagcgact ggagaagctc gaatcactgg ccacggcgat taagggtgtg
     5161 cagaaggcct atgcgatcca ggcaggtcgt gaaattcggg ttatcgtccg gcaggaagat
     5221 ctcactgatt cagagtcgtt ccaactgtct cgagacttgg cgaagaagat tgagcaagaa
     5281 ttgacgtatc ctggccagat caaagtgacg gtcattcgcg aaagtcggta cgtggaatac
     5341 gccaaatgaa agtcctcatc atcggcgaca tcatgggaga gcctggtcgc agagccatcg
     5401 caagagccat gcctcggttg gtgtctcaac ggcagatcga tattgtcatt ggcaacggcg
     5461 agaacatagc cggtggattt gggattacgc cagatttggc ggaagaactg ttcgagatgg
     5521 gattggcggt cattaccacc ggcaatcatg cctgggataa aaaggaaatc ctcgactatt
     5581 tccctcgtga gccgcgactc cttcgaccgg ctaattaccc cgctggcgtt cctggttatg
     5641 gtagttatgt gatcgagacc tccagcgggg aacatctcgc tgtgctccat ttgatgggcc
     5701 gggcttatat gccaacgctg gattgtccat tccaagtggc aagaaaagaa ttagtcgggc
     5761 tgaagaatca ggctgcagca gtcatcgtcg acatgcacgc tgaagctaca tcggaaaaaa
     5821 tggccatggg acactatttg gatggcgaag tgacggcggt ggttggtacc cacactcatg
     5881 tgcagaccgc cgatgagcag attcttccca agggaacggc ctatctgaca gacattggaa
     5941 tgaccggtcc actccattcc gtgatcggag tgaagaagga attggcgatc gaaaaatttc
     6001 ttaccgggat gccgcgtcgg ttcgaggtcg caagcggtcc cactgtattc tgcgcggctc
     6061 tagttgagtt ggatgctcgc ttgggtaagg cactgtccat cgaacggatt cggattatcg
     6121 actaacggag cccgctacgc acgcacgcga attctctgct cctctctttc ctgatacccc
     6181 gccgagacgg atcctgaccg ttggggaact cacggatctg attcggacgt cgatcgaaac
     6241 acagttttgg gacatctggt tggagggaga aatttccaat ctccgcacgc caggatccgg
     6301 acacctctac tgtaccctta aagataatac cagccagatt cgggcggtgc tcttccgatc
     6361 gaatggtcta cggttacgat ttgctttaca agaagggctg cacgtgattg tgcgcggtcg
     6421 gctcaccgtc tatgagccgc ggggggagta tcaaatcgtc cttgagcatg tcgaacctaa
     6481 gggaatcggt gcccttcagc tggcgttcga acaacttaag gagcggcttg ctgcagaggg
     6541 cctcttcgat caaaagcgga agaagtcact gccggcgttt cctcgcgctg tcggtctcgt
     6601 gacatcgatc gcgggagcgg ccatccatga catccttacg gtccttcaaa agcggtggcc
     6661 acttctccac atcatcatcg caccggtaca ggtgcaaggc gaaggagcag caaagcagat
     6721 tgccgacgcg gtggcttcat tgaacagcat tcgtaagatt gacgtcatta tcgtcggtag
     6781 gggtggagga tcctgggaag atctctggag ctttaatgaa gaggccgtcg tgcgggcaat
     6841 cgtggcgtcg cgtatcccag tggtatcggc ggtcggccac gagattgatg tgaccttggc
     6901 cgaccttgct gctgatgttc gggctccgac cccgtcagca gcagctgaaa tggtagtgcc
     6961 tgtgttgtct cacttggtag aacaactgga cgattatact gttcgaatgc agcgggtgat
     7021 gacacggcgc tgtgccttcg aacgtcatcg gctcgaaacc tatctgagtc agatatccca
     7081 tatgcgtttt tatgttcaag aaagatctca gcttgttgat gagctaacgg gtcgtctctg
     7141 tcatgctgtt gacgaactga ggagcatcat gaaacagcgt gtgcatggcc ggtatcaaga
     7201 tctcctcgct tgcaatccgc tccttttcgt caaacaaggc ctcaccctca ttcctcagct
     7261 tgtgacaaga ctccatcgac aaatgaaaag cgtgatactg catcgtcgcc agcgaattga
     7321 gcaggcggta gcggagctga ataacctcag ccccttagcc attcttgcac gcggttatag
     7381 cattcttcag aatgttggga caggtcgaat catccgtcat gcaaaagacg ttcgtgtcgg
     7441 ggacgagtt
//
LOCUS       DATDNW010000303         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_106450, whole genome shotgun sequence.
ACCESSION   DATDNW010000303 DATDNW010000000
VERSION     DATDNW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106450"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VI359_07260"
     CDS             complement(<1..815)
                     /locus_tag="VI359_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY6974086.1"
                     /translation="MNVREVLEFAKKNKVQIVDLKFVDLIGTWQHFSIPVNELTEGLF
                     KDGSGLDGSSIRGWKAINNSDMLVVPDPATACLDPFCSVPTLSLVGNVVDPISRETYD
                     RDPRFIAQKAEKHLQSTKIGDISYWGPEAEFFIFDQARYDQTSHSGYYFIDSEEGVWN
                     MGQEGFNLGGKIRHKEGYFPVPPTDTQQDIRSEMILEMEKVGIEIEKHHHEVATAGQA
                     EIDIRFDSLVRTADKMMMYKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQ"
     gene            complement(864..>2746)
                     /locus_tag="VI359_07265"
     CDS             complement(864..>2746)
                     /locus_tag="VI359_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY6974087.1"
                     /translation="LHVHGGMAQEILTFDEQIWLAERFGFTDQPHLLAVEQFMQRYYQ
                     HTMGLHERCIRFLERCKRIPLGQRLARFLPAPKVDEHFVIRGGMLTVTDEKRTRVLDS
                     PALLLKLFDVARRRQLTIDPPLLEDIHRHVEAASEDAYRTPEISQTFLNILAGPGTAK
                     TLEAMHRAYLLEKLVPAMGTVRGLMQFNQYHKYTVDEHSLLAVGKVEALAQDRAVLGD
                     VYREIKRKDILHLAVLLHDLGKGREEDHSEVGKRLAEEAAARFGFDEQETRSLVFLVY
                     RHLLMAHTAFRRDPNDVKVVLPFAREVGTPEVLKKLLALTAADIAAVGPGVLTKWKES
                     LLIELYLRTIPEVSGERESADIAAGLTDIATEVSRQPIVAALSSVDQAWIEGQLHQFP
                     QRYLYGTSVPRMAMHLAAVRRLHAGEVVVESEFNEELGTCEYTVIAHNDLTPGIFSKI
                     AGVMAGNGLQILDAQILTRADGIVIDTFQVTDPDYNGPPPAERRRLVGERVAAVLKGW
                     EHVDDVIRRGARLKLTRPLPKAREATEVRIDNETSDGFTIIDVFADDRQGLLYVMTNA
                     IFQLGLSVHAARISTRLDQVADVFYVTDQRGKKIEDHVQLERIRAGVEKAIENFLEAK
                     AA"
BASE COUNT          550 a          852 c          736 g          608 t
ORIGIN      
        1 ctctggtgcg tgtgcatgcc ggacccgttg tcgccgaaga tcggcttcgg catgaaggtc
       61 acggttttcc cgtgtcggcg cgcgacgttc ttgacgatgt acttgtacat catcattttg
      121 tcggccgtcc tgaccagcga atcgaaccgg atgtcgattt cagcttggcc ggccgtcgcg
      181 acttcatggt gatgtttctc gatctcgatc ccgacttttt ccatttccag aatcatctcg
      241 ctgcggatgt cctgctgggt atcggtcggt ggcacgggga agtagccctc cttgtgacga
      301 attttcccgc ccaagttgaa cccctcttgc cccatgttcc agacgccttc ctcggaatcg
      361 atgaagtagt agccgctgtg gctggtctgg tcgtagcgcg cctggtcgaa gataaagaat
      421 tcggcctcag gcccccagta ggagatgtcg ccgatcttgg tgctctgcag atgcttctcc
      481 gctttttgcg cgatgaatcg gggatcgcga tcgtaggttt ccctcgaaat gggatcgacg
      541 acgttgccga ccagactcag cgtcggcacg gaacaaaagg gatcgaggca ggctgtggca
      601 gggtccggga caaccaacat gtcgctgttg ttgatagcct tccaccctcg aatcgacgag
      661 ccatcgagac cggacccgtc tttaaacaat ccctccgtca attcgttgac gggaatgctg
      721 aaatgctgcc aggtcccgat gagatcgaca aacttcaagt ccacgatctg cactttattc
      781 ttctttgcaa actccaacac ctcacgaacg ttcattcccc tcctcctttc gacccctgct
      841 gagtgaatcc cttacagccc tgattaggcc gccttggcct caagaaaatt ctcgatggcc
      901 ttttcgacac ccgcccgaat ccgttcgagt tgcacgtgat cctcgatctt tttcccccgc
      961 tggtcggtga catagaacac gtcggcaacc tgatccaatc tggtcgaaat gcgcgccgcg
     1021 tgcaccgaca agcccagttg gaagatggca ttcgtcatca catagaggag cccctggcgg
     1081 tcatccgcaa acacgtcgat gatcgtgaac ccatcggacg tctcgttatc gatccgaacc
     1141 tccgtggctt cgcgcgcctt cggcaagggg cgcgtcagct tcaatcgggc gccgcgccgg
     1201 atgacatcat cgacatgttc ccagcctttt aagaccgcgg ccacacgttc accgacaagc
     1261 cggcggcgtt cagccggcgg gggaccgttg tagtcggggt ccgtcacctg aaacgtatcg
     1321 atgacaatcc cgtcggccct ggtgagaatc tgcgcgtcga ggatctggag tccgttcccc
     1381 gccatcaccc ccgcgatttt ggaaaagatc cccggtgtca aatcgttgtg cgcaatcacg
     1441 gtatattcac aggtgccgag ctcttcattg aactccgact cgaccacaac ttcgcccgca
     1501 tggagtcgcc ggacggccgc caggtgcatc gccatacgcg ggaccgacgt gccatacagg
     1561 taccgctgtg ggaactgatg cagttgccct tcgatccacg cctgatcgac gctagatagg
     1621 gcagccacga ttggctggcg actgacttcc gtggcaatgt cggtcagacc agcggcaatg
     1681 tccgccgatt cgcgctctcc cgacacctcc ggaatagtgc ggagatacag ttcgatcagc
     1741 agtgattcct tccatttcgt caagacaccg ggacccaccg ccgcaatgtc ggcagccgtg
     1801 agcgcgagga gcttcttgag cacttccggc gtacccacct cccgggcgaa cggcaggacg
     1861 accttgacgt cattcgggtc tcgtcgaaac gccgtatgcg ccatcaacaa gtgccgatac
     1921 accagaaaga ccagcgagcg tgtttcctgc tcatcgaatc cgaatcgagc agcagcctct
     1981 tccgccagcc gttttcccac ctcactatgg tcttcttccc tgcccttccc caagtcatgg
     2041 agcagcacgg ccagatgcag gatgtccttc cgcttaatct cccgatacac atcgcccaag
     2101 actgcgcgat cctgtgccag cgcttcgacc ttcccgactg cgaggagact atgctcatcc
     2161 acggtatact tgtggtactg attgaattgc atcagcccac gaacggtccc catcgccgga
     2221 acgagcttct cgagcaagta ggcccgatgc atcgcttcta acgtcttggc cgttccaggc
     2281 ccggccagga tattcagaaa cgtttggctg atctcaggtg tgcgataggc gtcttcggac
     2341 gccgcttcga catgccggtg aatgtcctcc aaaagaggcg ggtcaatggt cagttgtcga
     2401 cgtcgggcga catcaaagag ttttaacaag agggccggac tatccaggac gcgcgttcgt
     2461 ttctcatcgg tcaccgtcag cattccgccg cggatgacga agtgctcgtc cactttcggg
     2521 gcgggaagga atcgggctaa acgttgaccg agcgggatgc gtttgcaacg ctccagaaat
     2581 cgtatgcagc gttcatgcaa ccccatcgtg tgctggtagt accgctgcat gaactgttcg
     2641 accgccaaca gatgcggctg atccgtgaaa ccgaaccgtt cggccaacca gatctgctcg
     2701 tcgaaggtca gaatttcctg cgccattccc ccgtgcacat ggagca
//
LOCUS       DATDNW010000304         2922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_662268, whole genome shotgun sequence.
ACCESSION   DATDNW010000304 DATDNW010000000
VERSION     DATDNW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662268"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="ccsB"
                     /locus_tag="VI359_07270"
     CDS             complement(<1..890)
                     /gene="ccsB"
                     /locus_tag="VI359_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY6974088.1"
                     /translation="MMRSLFLFDMTFWLYLAALTLYIAYLFARRPVMTLAAAGHPTEN
                     FDERDGAWATQLGQVATLITVFGWIVNTAALFTRAFERLEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLELRYKIKAIGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAEQRGSRSRFFAAFPDLETIDELGYKAIMI
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGRRAA
                     IYAVFGFLMVIFCFWGVNFLL"
     gene            complement(973..2406)
                     /locus_tag="VI359_07275"
     CDS             complement(973..2406)
                     /locus_tag="VI359_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY6974089.1"
                     /translation="MNGQPDALPRKDAPRSSSPGLGWDEFSRELVEFFASIKLAMFLF
                     LFLAATATIGTVIQQGERPETYIQEYGENAYRWFLRLGLIDVYHTWWFTSILGLLCVN
                     SLTCFHKRFPAVWRSMRQDKVSVSLAFIKGMKQQAEVSVDAGKEAVAEKLVQHFAEKG
                     YRVLAKNEPGEVTVYATKGVLGRVGAHMAHLSATVIVLGGLLGSYYGFQEFGVCLEGQ
                     TYHIPRGNFDLKVDKFWIDYHENGSVKSYNSTLTVLEGGQFVLTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEVARINIKDKQTDKVVKTVDLEWQKEQAISDLKLKLSITDFVAD
                     FAFNSTEKKVFSKTVEHANPAIRLTVNERETVQATPWIFYQFPDLFEVKDSKYQFELV
                     GYKPKKYTGLQIAKNPGINIVWTGSTMIVVGITLSSFIYHRRLWAKIVPEGRGVVVHL
                     GGTTHKSQIDFQKEFGKLSDRIRTFSA"
     gene            2737..>2922
                     /locus_tag="VI359_07280"
     CDS             2737..>2922
                     /locus_tag="VI359_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY6974090.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKYSRVACE
                     TILTTGIAFVAGEISTKA"
BASE COUNT          734 a          869 c          719 g          600 t
ORIGIN      
        1 agcaaaaagt tcacacccca gaaacagaag atcaccatta agaatccgaa gaccgcatag
       61 atggccgccc tgcgaccttc ccacccacgc gtcatgcgcg catggatgta ggctccgtaa
      121 atcagccaca cgatcaacga ccaggtctcc ttcggatccc aactccaata gccgccccag
      181 gcataattcg cccacatcgc accgaggatg ataccgaagg caagaagcgg gaagccgatc
      241 atgatggcct tgtagcccaa ctcatcgatc gtctcgagat ccgggaacgc ggcaaagaac
      301 cgcgaccggc tcccccgctg ttccgcccgc tctttgaaca gatacatcag gccgagacca
      361 ccggccatgg caaaggccgc ataactggtc agggtcacgg acacatgaat ataaatccag
      421 tagctgttaa gggccggcac aagcggttca gccgtctggt agcgataggg taagagcgac
      481 gcggctccca tcgcgatgaa cccgatcccc accacgaacg ccccaatggc cttgatcttg
      541 taccgcagct caagcagcac gtatccaaga ataatggccc aagagacgta ggccatggct
      601 tcgaattgat tggaccaggg cgcgaaggtc cctgaatgtt cgaggcgttc aaaggcgcgg
      661 gtgaacaagg ccgccgtatt cacgatccaa ccgaacaccg taataagcgt ggcaacctgt
      721 cccagttgcg tcgcccaggc cccatcgcgc tcatcgaagt tctcagtcgg atggccggcc
      781 gccgccaatg tcatcaccgg acgtcgtgcg aagagataag cgatatagag agtcagggct
      841 gcgagataga gccagaaagt catatcgaat aagaaaagag accgcatcat aacgtcctcc
      901 gtcgatcaaa tcaggcaacc aagccagtca ctatcatacc caagagaggg gtggattgac
      961 caccatatgt cttcaagccg aaaacgtccg aatcctgtca ctcaacttgc cgaattcttt
     1021 ctgaaaatcg atctggctct tatgagtggt cccaccgaga tggaccacga caccccgtcc
     1081 ctccggcacg atcttcgccc agagacgccg atgatagata aaggaggaga gagtgatccc
     1141 gacgacgatc atggtcgacc ccgtccagac gatattaatc cccggattct tcgcaatctg
     1201 gagacctgta tacttctttg gcttataccc cacgagctcg aactgatatt tcgaatcttt
     1261 gacctcgaag agatcgggga actggtaaaa aatccacggc gtggcctgca cggtctcccg
     1321 ctcgttgacc gtcagtctga tcgcggggtt cgcatgttcg accgtcttgg agaagacctt
     1381 cttttccgtt gaattaaaag caaaatccgc tacgaagtcc gtgatcgaca gcttcaattt
     1441 gagatcgctg atcgcctgct ccttttgcca ctccaaatcc actgtcttga ccaccttgtc
     1501 ggtttgtttg tccttgatat tgatgcgcgc aacctcgatc tggtcccacg catcgccgta
     1561 gctggactgg taaaaccaga ttcccttgta gaccagcgga tcgttgaccg taatcgtctt
     1621 cgtgagcaca aactgcccac cctcaaggac cgtcagcgtg ctgttatacg atttcaccga
     1681 gccgttctcg tgataatcga tccagaactt gtcgactttc aaatcaaaat taccgcgcgg
     1741 aatgtgatac gtctgccctt ccaggcacac accgaattcc tgaaacccgt aatagctgcc
     1801 gagtaatccg ccgagcacga tgaccgtcgc gctcaggtga gccatgtgcg cccctacgcg
     1861 gccgaggacg cccttcgtcg catagaccgt cacctcgccc ggttcgtttt tcgcaagcac
     1921 gcgatagccc ttctcggcaa agtgctgaac gagcttctcc gctaccgctt ctttaccggc
     1981 atccaccgac acttccgcct gttgcttcat ccccttgatg aaggcgagcg agacactgac
     2041 cttgtcctga cgcatggatc gccagacagc gggaaagcgc ttgtggaaac aggttaagga
     2101 attgacacag aggagcccca gaatgctggt gaaccaccag gtgtggtaga cgtcgatcag
     2161 ccccaaccga agaaaccagc gataggcgtt ctctccatat tcctgaatgt aggtttccgg
     2221 ccgttctccc tgctgaatga ccgtgccgat cgtggccgtc gcagccaaaa agaggaagag
     2281 aaacatcgcc agcttaattg aggcgaagaa ctcgactaat tcacgcgaaa actcatccca
     2341 tcccaacccg ggactggatg accgcggggc atccttgcga ggaagcgcgt ccggctggcc
     2401 gttcatgatc gcttaatact ccctcctctg gtcatctgac acaggccctc atcatctatt
     2461 cgtggaaacc cctcgctttg aatctaggga ttgtccctgc cggaaaggcg agaccctgtt
     2521 cacattcagc tgcacaggcg gcgttgcggg atggctcgtt aagtccgcag aaatccagtc
     2581 atcttacaag cgctggaaaa ggggtgtcaa gcaacaagct gagacatctc agattgatcc
     2641 ccctcgcagg aaggaataga caaatgaggg gcagtcatac tactatcact cgaattcccg
     2701 aaagttttcg ttcaccagga tcttcgaagg agcgtaatga gacacaacta cctgtttacc
     2761 tcagaatccg taacggaagg acaccccgat aagattgcag accagatttc cgatgggatt
     2821 cttgatgcga tcatcgctca agataagtat tcacgagtag cctgtgagac catcctcacc
     2881 actggcatcg ccttcgtggc cggcgaaatc tccaccaaag cc
//
LOCUS       DATDNW010000305         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_662695, whole genome shotgun sequence.
ACCESSION   DATDNW010000305 DATDNW010000000
VERSION     DATDNW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662695"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VI359_07285"
     CDS             complement(<1..515)
                     /locus_tag="VI359_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6974091.1"
                     /translation="MTNSGILDVRPTKKRSKIPFTILIVEDNATDVELMLHALEHAEL
                     KPLGGDFDMEVRATAEGAIELLKERAVDLVLTDMMLPGMSGLDLVSRIQTIDPNLPVL
                     VVTRVNTVSQAVDAMRRGAFDYVLKPVNAEDLGVRLHRAIRISEILRRHAVYEQHDRQ
                     DYEANSLVGVSP"
     gene            complement(524..2620)
                     /locus_tag="VI359_07290"
     CDS             complement(524..2620)
                     /locus_tag="VI359_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6974092.1"
                     /translation="MDRWAAQWWDQLRIQHKVWAVLLLLCLPLIGGLAIHLYFIDQLL
                     TVQQQRHDLVLASEQVEQLGRLAVDVEDSFRGYVLTQQPAFLAPLTDADAKIGPALTK
                     AAKTLASLPGSPDGLGPIDQQLKTLLRSKHELIADIQRGDAAKALAYIRSGEGLQLSE
                     SLRHDLRIIEDGLDHRRELHTAKAQLLSQRTYIGLWIALAGVVVLGWISSHMLARSLT
                     DPIRRLQSATTKFGAHADPDEITNLLVSARASKDELGQLADAYLTMAQRIGTHIREVE
                     VLDLIGYDINTIGPDGLDGVLRRISDRAVELVGADVCLVLLRDERMGCWIVEAASGQW
                     DEKLKKSVMLWEELPVCVQAFESGQPAMNEQGRGNQHPAVLRRALIGDNMLAIPLHAQ
                     GKPFGVLALVSERSRTAQEWNQRLAKGMTQEAALAISNARLYEAAQQKQRGLLARLRQ
                     LEYLAETLAHDLKGPGARMEELAKLLVQQFAGQIDERASRWLKLIQDNGSDLVQRVEG
                     ILAVARVGVGQGSVTAVDPTLVISEVLKSHAGEIERLRAKVHIELGLPLVACHGAYLR
                     QVFDNLILNALKYARPGEPPSITVSHQIENHTVCFSVTDQGIGIPEEQRARVFQPFVR
                     LLQAESTGSGIGLTIVQRIVELYGGSVWIEGNGHDGCTVKFTVPWLRDEGGALMTGTK
                     VSDLPEMVDISSKGLI"
     gene            complement(2804..3475)
                     /locus_tag="VI359_07295"
     CDS             complement(2804..3475)
                     /locus_tag="VI359_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6974093.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKSKDDINVVGEAQDGIEAVEK
                     TKALLPDVILMDVSMPRMGGVEATRQIKREFPHIGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMAQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANNLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LDPRP"
BASE COUNT          712 a         1076 c          922 g          811 t
ORIGIN      
        1 ggactcactc ccaccagact gttggcctca tagtcttggc gatcatgctg ctcgtagacg
       61 gcatgccggc ggaggatctc agagatgcgg atagcccgat gcaaccggac acctaaatcc
      121 tcagcgttga ccggcttcag aacgtaatcg aacgcccctc ggcgcatggc atcaaccgct
      181 tgtgaaaccg tgttgaccct tgtgacgacc agcacgggta agttcggatc aatcgtttgg
      241 atgcgactga cgagatccag tccgctcatg ccgggaagca tcatatcggt caacacgagg
      301 tccaccgcgc gctctttgag caactcgatc gccccttccg ccgtcgcgcg aacttccatg
      361 tcgaaatcgc cgccgagggg cttcaattcc gcatgttcca gcgcgtgcag catcagctcc
      421 acatccgtcg cgttatcttc cacgatcaag atggtgaacg gaattttgga acgtttcttc
      481 gtcggacgga cgtcaagaat tccgctattc gtcatgcgtc acctcagatc aatcccttcg
      541 aagagatatc caccatctcc gggagatcgg acaccttggt tccagtcatc aatgcgcctc
      601 cctcatccct caaccaaggc acggtgaact tcaccgtgca tccgtcatgt ccgttccctt
      661 cgatccacac actcccgcca tagagttcta cgatccgctg tacgatcgtc aagccgattc
      721 cgctgcccgt cgactccgcc tgcaataatc tgacgaatgg ttggaacaca cgggcacgct
      781 gctcctcggg gattcctatc ccctggtctg tcactgaaaa acagaccgtg tgattctcaa
      841 tctggtgact cactgtgatc gaaggtggct ctccaggacg cgcatatttt aaggcgttta
      901 aaatgagatt atcgaacact tgccgaaggt acgccccgtg gcaggcgaca agcggcaaac
      961 ccagttcaat gtggaccttc gctcttaatc gctctatttc ccctgcatgg ctcttgagta
     1021 cttcgctgat gacgagagtc ggatccaccg ccgtcaccga cccctgtccg accccgacac
     1081 gagccaccgc cagaatccct tcgactcgct gcacgaggtc gctgccattg tcttgaatga
     1141 gcttcagcca tcgtgatgcc cgctcatcaa tctgtccggc aaactgctgc accaacagtt
     1201 tggccaattc ctccatccgc gctcccggcc ccttgagatc atgggccagg gtttccgcca
     1261 gatattcaag ctgtcgcaat cgcgcgagca agccccgttg tttctgctgt gcggcttcgt
     1321 acagccgcgc gttcgagatg gctagcgcgg cttcttgagt cattcccttg gcgaggcgct
     1381 ggttccattc ttgggccgtc cgagaccgtt cactgacgag ggccagcacg ccgaatggtt
     1441 tcccttgcgc gtgcaaaggg atggccaaca tgttgtcgcc tattaaggcc ctgcgtagaa
     1501 ccgccggatg ttggttcccc cgaccctgct cattcatcgc gggttggccg ctctcgaacg
     1561 cttgcacgca gacgggcagt tcctcccaca gcatcacaga cttcttgagc ttctcatccc
     1621 actgtcctga ggcggcttcg acgatccagc atcccatccg ctcatcacgg aggaggacca
     1681 gacagacatc cgcccccacg agttcaactg cacgatcgga gatccgacgc aggacgccat
     1741 ccaatccgtc gggaccgatt gtgttgatgt cgtacccgat caggtcgagt acttcgactt
     1801 ctcgaatgtg ggttccgatg cgttgggcca tagtcagata cgcgtcagct aactggccga
     1861 gctcgtcctt cgaagcccga gctgagacga gcaggttggt gatctcgtcg ggatcagcat
     1921 gcgctccgaa cttcgtcgta gctgattgca gtcgcctaat cggatcggtc agggaacggg
     1981 ctagcatgtg cgaactaatc caccccagca ccaccacacc tgccagtgcg atccacagtc
     2041 cgatgtacgt ccgttgcgag agtaattgtg ctttggcagt atgcaactct cgtcggtgat
     2101 cgagaccgtc ctcgatgatt cggagatcat gtcgaaggct ttcggacagc tgaagccctt
     2161 cccccgaccg aatatacgcc aacgccttcg ctgcgtcccc ccgctgaata tcggcaatca
     2221 attcatgctt cgatcgcaaa agagttttca gttgctggtc aatcggcccg agaccgtccg
     2281 gagaacctgg cagacttgcc aacgtcttcg ccgccttggt gagagccgga ccgattttcg
     2341 cgtccgcgtc ggtcagcggt gcgaggaacg caggttgctg ggtcaacaca tacccgcgaa
     2401 aactatcctc aacatcgacg gcaagccgac ccaactgctc cacctgctcg ctcgccagaa
     2461 cgaggtcgtg tcgctgctgc tggacggtga gtagttggtc gatgaaatag aggtggattg
     2521 ccaggcctcc gataagcggg agacagagca gcaagaggac cgcccacacc ttatgttgga
     2581 tacggagctg atcccaccac tgtgcggccc atcggtccat cggacggcgc cctctcgggt
     2641 cgggactgtg tgtcggaaac tatcgtaaaa tctttattat attggaaccc cgaaaaatca
     2701 aatgtcaagc cgtgcacgca cgggatgagg gagattatcc gacacttcct agggcctccg
     2761 ctcggagata acgttcctat cgtttccgtc acgacgcgaa acgctagggt cgcgggtcca
     2821 actcgatgag tcctttgcgt atggcaagga ttgcggcttg tgtgcggtca tagacttgca
     2881 gtttgtggaa gatgttgcgg acgtggtttt tgaccgtttt ctcgctgaga tcgagattgt
     2941 tggcgatttc cttgttggtc tttccgtcgg ccactaagcg caagaccgtg atctctcgtt
     3001 cggtcaaatc gtgctccacc caggctggct tcttgccctt tttctgcgcc atcaacgaaa
     3061 actccgccag gatcttgctc gccacggagg gatgaatcag cgattcgcct cgataaatcg
     3121 cgcgaatggc ggcgacgatt tgcgaggatt ctgagtcctt gagtagatag ccggtggcac
     3181 cggcccgcac caagtcgaag atgtattgct gctcttcgta catggtcagc gcgacgatgc
     3241 cgatgtgggg aaactcgcgc ttgatctgcc gcgtcgcctc tacaccaccc atgcggggca
     3301 tgctcacatc catcaggatc acgtccggga gcaaggcttt agttttttcc acggcttcta
     3361 tcccatcctg tgcttcgccc accacattga tgtcgtcctt tgatttgagg atagctgcca
     3421 gcccttctct cacgacgcga tggtcgtcgg cgattagtac cttaattttt tccattgtgt
     3481 gccgcctcct tgttcaccaa tgggacatcg accacaatcg t
//
LOCUS       DATDNW010000306         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_515433, whole genome shotgun sequence.
ACCESSION   DATDNW010000306 DATDNW010000000
VERSION     DATDNW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515433"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VI359_07300"
     CDS             complement(<1..515)
                     /locus_tag="VI359_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974094.1"
                     /translation="MQTLDIDRPNMPDLQFALLVTALCTSRLTALNIPNDLRTAIFDR
                     CWVLIHDSPPPGKPEERVLDLRSWTEVTVEAMVETIRSALTEAGIRTLTWDHPPSEPT
                     HISTPEALPLIERLEQLYPQPSKASDAVSGEALVVSRGLDDHGPDVRQLIGRMAALIT
                     AFSHALELCAA"
     gene            complement(590..832)
                     /locus_tag="VI359_07305"
     CDS             complement(590..832)
                     /locus_tag="VI359_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974095.1"
                     /translation="MTDRQPNTPFAKPPVRYTLSYGMRLDKGTGSELSHPHVPVILPN
                     GSAGSMALHVMNGTPDEIKARLLESVDAFFEIYTDL"
     gene            complement(933..1823)
                     /locus_tag="VI359_07310"
     CDS             complement(933..1823)
                     /locus_tag="VI359_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6974096.1"
                     /translation="MSLDYVKFSPGFDAFMPKEYRDMVEHGPFGKKTTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAIIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRIRFGDQPKDVVVMAGDGGTADIGFQQVLHSWFRQERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVSMAKIAGCAYIATVVPNNPRRVESCI
                     KKAVLIAREVGPTYIQAYTSCNIEYAIPTDKVMEDAKTVEDDRYKFTEYVSEEAKAYL
                     AERYGYREFLPKPAAALVKA"
     gene            complement(1868..>2090)
                     /locus_tag="VI359_07315"
     CDS             complement(1868..>2090)
                     /locus_tag="VI359_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HEY6974097.1"
                     /translation="IGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYIS
                     EPAKALLAELAAKAKEKKDAAKQLALQEA"
BASE COUNT          376 a          613 c          598 g          503 t
ORIGIN      
        1 tccgctgcgc acagctccaa ggcatgggag aacgctgtga tcaacgcggc catcctgccg
       61 attaactgcc tgacgtcagg tccgtggtca tccaggcccc gtgaaaccac cagggcctct
      121 ccactgacgg cgtcactcgc cttggatggt tgaggataca gttgctcgag tcgttcgatc
      181 aggggtaggg cttctggtgt gctgatgtgt gtcggttcgc tgggtggatg atcccaggtg
      241 agtgtccgga tacccgcctc cgtgagtgcg cttcgtatgg tttcgaccat cgcttccacc
      301 gtcacctcgg tccaggaccg caagtctagc acccgctcct ccggcttgcc gggcggcgga
      361 ctgtcgtgga tgagcaccca acagcggtca aaaatcgctg tgcggaggtc attcggtatg
      421 ttgagcgcgg tcaggcggga tgtgcagagt gcggtgacca acagagcgaa ctgaagatcg
      481 ggcatgttag gacgatcaat atcgagcgtc tgcatgatct gttgcctccc acgtaaggcg
      541 tcggaacagt ccttcgcgga gacgacgcct cggtcacgaa cggttgggct cagaggtcgg
      601 tatagatttc gaagaacgcg tcgacacttt cgagcagtcg cgctttgatt tcgtccggcg
      661 tgccgttcat cacgtgcagc gccatgctgc ccgcagatcc gttcggcagg atgacgggca
      721 cgtgcgggtg gctcagttct gacccggtgc ccttatccag ccgcatgcca tagctcagcg
      781 tatagcgtac cgggggcttc gcgaacggcg tatttggttg cctgtctgtc atgatccacc
      841 tcgggtccga tcgtctacag ggcctgttgg ctgacgggtg gaagattcgg gcaattcgtg
      901 tgggccatcg cccgaatctt ctgcccatgt cgttatgcct tcacgagagc agcggccggc
      961 ttcgggagaa actccctgta gccataccgt tcggccaggt acgccttggc ttcttcgctg
     1021 acatactcgg tgaatttgta gcgatcgtct tcgacggtct tggcgtcttc catgaccttg
     1081 tcggtcggga tcgcatactc gatattgcag gacgtgtagg cttgaatata ggtcggaccg
     1141 acttctcggg cgatcagcac cgctttcttg atgcagcttt ccacccgccg cggattgttt
     1201 ggcacgaccg tggcgatata ggcgcagccg gcgattttgg ccatgctgac catgtccatc
     1261 ttctcaaact tcttgccgag gggggccatc ttgagcaccg ctcctctggt cgtcatcccg
     1321 ctttcttgcc cgcccgtatt gccgtacacc tcgttatcca acatgatcgt tgtgaaccgt
     1381 tcctgccgga accaggaatg gagcacctgc tggaatccga tgtcggcggt tcccccgtca
     1441 ccggccatca ccacgacatc cttgggctgg tcaccgaatc ggatccgcaa cccgcgtgac
     1501 aatccgctgg cgacaccgtt ctgatcgccg taattgccat acacgaaggg aatcgccgcc
     1561 tgcgaaatgg ccagccggcc gcatccagcc gttccgaccg taatcgtgtc ttcgggattt
     1621 ggaaacgcga tgatggccaa ccggatgaac agggtcatgg cacagccggc acacatcgga
     1681 tgctcttcaa gaatttcttt gaagctgccc atttgcgaca cggtcgtttt ttttccgaag
     1741 gggccgtgct ccaccatatc ccgatattct ttcggcatga acgcgtcaaa tcctggtgaa
     1801 aacttcacat agtcgagact catctaacac ctcgcttttt ctgttagccg caattgcggc
     1861 ggtgaactta tgcctcttgc aaagccaact gcttcgcggc gtccttcttt tccttcgcct
     1921 tggcggcgag ttcagctagc agcgccttcg caggctcgct gatgtattcc ttgtaggcga
     1981 agcgttcggt gggtttctcc gaatcacgca tttcctgtaa gccttccatg ctgttcttgc
     2041 cgatctccag gatgcagggc gtatacaact gaagataggt gggtccgatt
//
LOCUS       DATDNW010000307         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_462066, whole genome shotgun sequence.
ACCESSION   DATDNW010000307 DATDNW010000000
VERSION     DATDNW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462066"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VI359_07320"
     CDS             <1..229
                     /locus_tag="VI359_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6974098.1"
                     /translation="EQQRVAVARALMQRPDLVLADEPTGNLDTHTGEALFALMRDLNK
                     SRGTTFVIVTHNEKLSAQADRIVYMQDGQIV"
     gene            300..1439
                     /gene="traF"
                     /locus_tag="VI359_07325"
     CDS             300..1439
                     /gene="traF"
                     /locus_tag="VI359_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HEY6974099.1"
                     /translation="MRIHTAMGMVAASMVVVLVLPGQVVAVEFVPVGPRAAGMGGAGV
                     AVTTDAYATYWNPAGLAMVKSLDIRIQGTVQVTGRNDIINALDDLKDFNPNDTSAANQ
                     AKAQSIANQINRPGATISGLGAGGLYFKGHFGEHAFGFNVSDVATSGGFVSSPIQVTT
                     GTQVNVAGQMALRGLEARQAAFSYAYAFADKTFSVGVTGKIIQGAAYNNTTNLQGGAN
                     PSLFNNFGQAMISTSYGIDLGAMYRPSSWLKFGVVGKDINQPSFDAPGGLQYKLTPQV
                     RGGVAVNPWSTMTLTADMDITSNQTLVPGLKSQVLSLGMEQTMLSEFLSIRVGTYKNV
                     QDAGSIFTPTAGLGIRIYSFRLDLAGGYDFDKGGAIASGALAWTF"
     gene            1455..2066
                     /locus_tag="VI359_07330"
     CDS             1455..2066
                     /locus_tag="VI359_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY6974100.1"
                     /translation="MIIFFLHTGLRIALGFSLLISAGCGGLQEVWEGPGARVFRPQAI
                     AVLPPMASQYDSAREDIQEVISSALNRIAHIERVVAPEQVTDVFQGSKEAFDALVFYF
                     SRFEMTGQSDKESAVKLGKALNVDSFLVVRVNSWEYVRKEGDNVGRVGLSLRLIDATN
                     GTTVWKARHERSSSYMFYKPNLKDVAKDLVDEMITQMPPQPKH"
     gene            complement(2170..>2788)
                     /gene="trpA"
                     /locus_tag="VI359_07335"
     CDS             complement(2170..>2788)
                     /gene="trpA"
                     /locus_tag="VI359_07335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY6974101.1"
                     /translation="AERALRNGVSLRKIIPMVKTLRNKTQIPLVFMAYYNTIHAFGPE
                     RFCREAVAAGVDGLIVPDMPPDEAGPLEEPAAETGLQLIFLVAPTSTTERRSYVVRRS
                     QGFVYYVSLTGITGAKLSNLTDVRANVEKIRKISTIPVAVGFGVATPEDAAKVAAIAD
                     GVIVGSAIVKRIAAHQQDNGMVIQVGSFVKSLKAAMVSSSITTSR"
BASE COUNT          612 a          782 c          770 g          624 t
ORIGIN      
        1 tgagcagcaa cgagtggccg tcgcacgggc tttgatgcag agaccggact tggtgctggc
       61 ggacgaacca acgggcaatc tcgacacgca cacaggcgaa gctctttttg ccttgatgcg
      121 agacctcaat aaatcccgcg ggaccacctt cgtcatcgtg acccacaacg aaaagctctc
      181 cgcccaggcc gaccgcatcg tgtacatgca ggacggtcag atcgtctaac tcttgacggc
      241 atacggtccc tccaatagac tcagcaaaag ccccgatgct tcaagggaga cgttattcga
      301 tgcgcatcca cacggccatg gggatggtgg cagcctctat ggtcgtcgtc ctcgtcctac
      361 ctggacaagt agtcgctgtg gagttcgttc ctgtgggtcc gcgcgcggca ggtatgggcg
      421 gggctggcgt ggcggtcacg accgacgcct atgccaccta ttggaacccg gccggattgg
      481 ccatggtcaa atcactggat attcgaattc aagggacggt tcaggtcact ggtcggaacg
      541 acatcatcaa tgcgctagac gatctgaagg acttcaatcc gaatgatacg tcggcagcca
      601 atcaggcgaa agcccagagc atcgcgaatc agatcaatcg ccccggtgcc accatctccg
      661 ggcttggtgc gggtggcctc tatttcaaag gacatttcgg cgagcacgcc tttggattca
      721 atgtctccga tgtcgcgacg tcaggagggt tcgtgagttc accgatacag gtgacgacgg
      781 gcacccaggt gaatgtagcc ggtcaaatgg cattacgggg attggaagct cgccaggcgg
      841 cattttccta tgcttatgcc tttgcggata agactttttc cgtcggtgtc acgggcaaga
      901 ttattcaagg cgcggcctac aacaatacga ccaaccttca gggcggagcg aacccgagtc
      961 tcttcaacaa ttttggacag gccatgattt ccacttccta cggcatcgat ctcggcgcca
     1021 tgtatcgccc ttcttcatgg ctgaaattcg gcgtggtggg taaggatatc aatcaaccga
     1081 gcttcgacgc acctggcggc ctccaatata aactcactcc ccaggtacga ggcggagtgg
     1141 cggtcaatcc ctggtcgacg atgaccctta cggcggatat ggacatcacg tccaaccaga
     1201 ctctggtgcc gggactgaag agccaggtct tgagcctcgg catggaacaa acgatgctgt
     1261 ctgaatttct ttcgatccga gtgggaacgt ataagaacgt tcaggacgcg ggatccatct
     1321 ttacaccgac tgcaggactc ggaattcgaa tctattcctt ccgtctcgac ctcgctggcg
     1381 gttacgattt tgataaggga ggcgcgattg cgtccggcgc tctcgcttgg acgttctgat
     1441 ggtataatga aaaaatgatt atcttctttc ttcatacggg tcttcgaata gcactcggct
     1501 tctcattatt gatcagcgcc ggttgtggcg gccttcaaga ggtctgggaa gggccggggg
     1561 ccagagtctt tcgcccgcag gccattgccg tgctcccacc catggcgagt caatacgaca
     1621 gcgcccgtga agacatccaa gaggtcatct cctcagccct caaccgtatt gcacatatcg
     1681 agcgcgtagt ggctccggaa caggtgaccg atgtcttcca aggatcgaaa gaagcttttg
     1741 atgctctggt gttttacttt tctcgcttcg aaatgaccgg ccagtcggac aaggaatcag
     1801 cggtgaagtt gggcaaagcc ctgaacgtcg attctttcct ggttgttcga gtgaattcct
     1861 gggaatatgt gaggaaggaa ggagacaacg tcgggcgtgt gggactgagt ctccgattga
     1921 tcgatgccac caatggcacc acagtctgga aagctcgcca cgaacggtcc agcagttaca
     1981 tgttctacaa accgaacctg aaggatgttg ccaaagatct tgttgacgaa atgatcacgc
     2041 agatgccgcc gcaaccgaaa cactagccgc ttctctgggt cgtcctccct cacggatctc
     2101 gcagcgcatt cgcttccgac caacggttga atcgatttcc gtcgatcaag aagtccggga
     2161 agaaggctgt tagcggcttg tcgtgattga ggatgaaacc attgcggctt tcagagactt
     2221 gacgaaagat cccacctgaa tcaccatccc attgtcctgt tgatgcgctg cgatccgttt
     2281 gacgatcgcg ctgcccacga tgactccgtc agcaatcgca gccaccttag ctgcgtcttc
     2341 gggcgtcgca accccgaatc ccaccgccac cggtatcgtt gatatctttc tgatcttctc
     2401 cacgttggcc cggacatcgg tgaggttgct gagtttcgct cccgtgatcc cggtgaggga
     2461 cacatagtaa acgaatccct gagatcgacg gacgacatat gagcggcgtt ctgtcgtgct
     2521 tgtcggggcc acaagaaaaa tgagctgcaa gccggtctca gcggctggct cttcgagagg
     2581 tcctgcttcg tccggcggca tgtccgggac aatcaaacca tcgacgcccg ccgccaccgc
     2641 ctcccggcaa aatcgctctg ggccaaacgc atggatcgtg ttgtagtaag ccatgaagac
     2701 aagtggaatc tgtgtcttgt tccgaagcgt cttcaccatc ggaatgatct tccgcagcga
     2761 tacgccgttc cggagcgccc gttccgct
//
LOCUS       DATDNW010000308         3256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_663162, whole genome shotgun sequence.
ACCESSION   DATDNW010000308 DATDNW010000000
VERSION     DATDNW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663162"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VI359_07340"
     CDS             complement(<1..603)
                     /locus_tag="VI359_07340"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY6974102.1"
                     /translation="MYRNLRLVPQLIFGRGSINQLGDILTKQRTESGCPVVFLIDAVH
                     EKKPFVHRLPLRANDLIIFVDVGTEPKTQYVDELTERVKAFSNRQPDAIVGIGGGSAM
                     DLAKSVSLMLTNPGSAADYQGWDLVKYPAIYHVGIPTLAGSGAEVSRTAVLTGPVKKL
                     GINSDFTPFDQIVMDPDLIASVPSNQRFFTAMDCYIHSCES"
     gene            complement(613..1371)
                     /gene="kdsB"
                     /locus_tag="VI359_07345"
     CDS             complement(613..1371)
                     /gene="kdsB"
                     /locus_tag="VI359_07345"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY6974103.1"
                     /translation="MNVAIIVPARIGSTRFPKKLLHQIKGKPVIVWTADRIRKEAPEF
                     PLYFAVDHESLAEVLRKEGFEAIMTDPSHSCGTDRIAEANRRLKAQYVINVQADEPLV
                     TGDQIRQLVELIQHDTEMATLGTPVQYDKDYRNPNHVKLVCDENGYAVYFSRAPIPYF
                     RDTHGAFEAEIAAGIPVLIHLGLYAYTASFLELFCELPPGKLEQVEKLEMLRAMERGH
                     RIKVGITKDALVEIDTPEQAVEFEQVVTQRFPSY"
     gene            complement(1406..2590)
                     /locus_tag="VI359_07350"
     CDS             complement(1406..2590)
                     /locus_tag="VI359_07350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY6974104.1"
                     /translation="MPGFELIGEEERREVQQVLESGVLMRYGFDAARNGQWKSRDLEQ
                     ALATRFGTQHAHACSSGTAALSTALAACGVGAGDEVILPPFTFVADLETVLLAGAVPV
                     FAEIDQTLCLDPRSVAAAITPKTKAVLVVHMCGAMARIDALVELCRAHRLLLIEDVAQ
                     AAGASFRGKPLGTFGKIGCFSFDYVKTVTCGEGGAIITDDAQIYNLAQAFTDHGHDHL
                     GADRGADNHPHIGTNYRLSELNAAVGVAQLAKLDRILETQRAHKAQLKDGLADIRRLQ
                     FRELPDPAGDSATFLSFMLPTEDHARQAAKSLGAAGVDGCFYWYDNNWHYHRKWDHIK
                     TLASPGRLPIDRFGYGADLTKIALPQSDAIMGRTISMLIKLRWTQDQVRDRLARMKSV
                     LA"
     gene            complement(2808..>3256)
                     /locus_tag="VI359_07355"
     CDS             complement(2808..>3256)
                     /locus_tag="VI359_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974105.1"
                     /translation="SSQPASSAEDTTSPAASSVQKGVQIPLAEIGPADGEYVRATGYG
                     NLAKGLYLCQHSADLAARVELSKLVRVQVKEKSVDRIREKTGKDAEQDIEVVREGLVN
                     EVLSDVRVVDRKVDKEAGTCSSTAVIPRRNLFPATPRPDATISAPQ"
BASE COUNT          626 a          955 c         1001 g          674 t
ORIGIN      
        1 cgactcacag gagtgaatgt agcaatccat cgccgtgaag aaccgctggt tcgacggcac
       61 gctcgcgatc agatcgggat ccatgacgat ctgatcgaag ggcgtgaaat cagagttgat
      121 gccgagtttc ttcaccggtc ccgtcagcac cgcggtgcgt gagacctccg ctccgcttcc
      181 cgccagcgtc ggaatgccga catggtagat cgcggggtac ttgaccagat cccagccctg
      241 gtaatcggcg gccgagcctg gattggtcaa catgagcgag acggacttgg cgagatccat
      301 ggcgctgccg cctccgatcc cgacgatcgc gtccggctgg cggttcgaga aggctttcac
      361 ccgttcggtg agttcgtcga cgtactgggt cttgggctcc gttccgacgt ccacgaagat
      421 gatcaggtca ttggcgcgga gcggcagccg gtgaacgaat ggctttttct cgtggacggc
      481 gtcgatgaga aacacgacgg gacagccgct ctcggtgcgt tgcttggtca gaatatcgcc
      541 gagttgattg atgctgcctc gcccgaagat cagctgaggg accaggcgca ggtttcgata
      601 catactcaca tcctagtaag aggggaatcg ttgggtcacg acttgttcga attcaacggc
      661 ctgctcggga gtatcgatct cgaccaacgc atcctttgtg atccccactt taattcggtg
      721 ccctcgttcc atggcgcgca acatctccag cttttcgacc tgttccagct tacccggagg
      781 aagttcgcag aacagctcga ggaaagaagc cgtgtaggcg tacagcccga ggtgaatcaa
      841 caccggaatg ccggccgcga tctccgcttc gaaggctccg tgcgtatcgc ggaaataggg
      901 gatcggcgca cgagagaaat acacggcgta accgttctcg tcgcacacga gtttgacgtg
      961 gttcggattt cggtaatcct tgtcatactg caccggcgtg cccaacgtgg ccatttccgt
     1021 gtcgtgctga atgagttcga caagctggcg aatttggtcc cccgtcacca agggttcgtc
     1081 cgcctgcacg ttgatcacat actgggcctt gaggcggcgg ttcgcttcgg caatccgatc
     1141 cgttccacaa gaatggctcg gatcggtcat gatcgcttca aatccctcct tgcgcagcac
     1201 ctcagccagc gattcgtggt ccacggcgaa atagagcggg aattccggcg cctctttcct
     1261 gatgcggtcc gcagtccaga cgatgacggg tttccccttg atctggtgca gcagtttctt
     1321 ggggaagcgc gtagagccga tgcgggcggg aacaataatg gcgacattca tggagggctc
     1381 ggattcgagt cgaacgacga cggcgctagg ccaaaaccga tttcatgcgc gcgagtctat
     1441 cacgcacctg gtcctgcgtc caccgcagtt tgatcaacat cgagatggtc cggcccatga
     1501 tggcatcgga ctgagggagc gcgatcttcg tcagatcggc gccgtacccg aaacgatcga
     1561 tgggtaaccg gccgggagac gcgagcgtct taatgtgatc ccacttccgg tggtaatgcc
     1621 agttgttgtc gtaccaatag aagcagccgt cgacgccggc ggctcccaag gatttggccg
     1681 cctggcgggc gtggtcttca gtcggcaaca tgaaactcaa gaaggtggcc gaatctcctg
     1741 ccgggtcggg aagctcacga aactgcagcc ggcggatgtc tgcaagaccg tccttgagct
     1801 gggccttgtg ggcacgttgg gtttccagga tgcggtcgag cttggccaac tgagcgacgc
     1861 cgaccgcggc attgagttcg ctgaggcgat agttcgtgcc gatgtgcggg tgattgtccg
     1921 caccgcggtc cgcaccgaga tgatcatggc catgatcagt aaaggcctgc gcgaggttgt
     1981 agatctgagc atcgtcggtg atgatcgccc cgccttcacc gcacgtgacg gttttcacat
     2041 aatcgaagga gaagcatccg atcttcccga atgtgccgag cggcttgcct cgaaagctcg
     2101 cgcccgcggc ctgggcgacg tcctcgatca gcaggagccg gtgcgcacgg cacaattcaa
     2161 ccaatgcatc gatccgcgcc atggctccgc acatatggac caccagcacg gccttggtct
     2221 tcggcgtgat ggcagccgcg accgaccgtg ggtccagaca taaggtctgg tcgatctcgg
     2281 cgaagacggg cacggcgccc gcgagcagca ccgtttcaag atcggcgacg aaggtgaagg
     2341 gagggaggat gacctcgtca ccggccccga ccccgcacgc cgccaaggcc gtgctgaggg
     2401 cggccgtgcc gctggagcac gcgtgggcgt gttgcgtgcc gaaacgagtc gccagggcct
     2461 gttcaaggtc gcgtgacttc cattggccgt ttcgtgctgc atcgaacccg tagcgcatca
     2521 acacgccgga ctcgaggact tgctgaacct cgcggcgttc ttcttcacca attaattcga
     2581 atcctggcat aatctccctc gcttcggctt actccgactc gcgcaggacc accgttcgtg
     2641 cgcgaggtcc attgtactga cgagggtctg cgatatccag cgcgaatctc tgtttcgaga
     2701 tgtggctcgt gaacccgctg agccgcaaaa cgagaagcgt ctctttttag gaatgaacga
     2761 ctgatggcct gtttgatggg cgccaattcc gggaggacgt cgctcgttta ctggggagca
     2821 gagattgtgg catcgggacg aggagttgcc gggaaaagat tccgccgcgg aatcaccgcg
     2881 gtgcttgaac aggtacccgc ctctttgtcc actttgcggt ccacgacccg cacgtcgctc
     2941 aggacttcgt tcaccaggcc ttcgcggacg acttcgatat cctgttcggc gtcctttccg
     3001 gtcttttccc ggatacggtc caccgatttc tctttcacct ggacacggac gagcttcgat
     3061 agctcaacgc gtgcagccag atcggccgaa tgctggcaca ggtagagtcc cttcgcgaga
     3121 ttgccgtacc ctgtcgcgcg gacgtactcg ccgtcggcgg gaccaatctc ggcgagcgga
     3181 atctgcacgc ctttttgaac ggacgatgcc gccggggacg tcgtgtcttc agccgatgag
     3241 gccggctgag aggatg
//
LOCUS       DATDNW010000309         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_663760, whole genome shotgun sequence.
ACCESSION   DATDNW010000309 DATDNW010000000
VERSION     DATDNW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663760"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VI359_07360"
     CDS             <1..216
                     /locus_tag="VI359_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974106.1"
                     /translation="PAHATLVQRGVARFLQRQRLARHAISILKGDRPQVASGVREGNV
                     SARRFGSALGRDIPQEIVQSPEEDQEF"
     gene            complement(325..732)
                     /locus_tag="VI359_07365"
     CDS             complement(325..732)
                     /locus_tag="VI359_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6974107.1"
                     /translation="MSLGQVLVVDDDPDILDTVRLVLRRAGYEVLTAANAQEAISLME
                     RDSHATTVGALLCDLDMPQIKGTELIAHFHARYPTIPITVLSGADAFQFTEAVVKQGV
                     TDWLRKPASREALLEKVRVSVRLHDLRAKESRQ"
     gene            complement(1043..1507)
                     /locus_tag="VI359_07370"
     CDS             complement(1043..1507)
                     /locus_tag="VI359_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY6974108.1"
                     /translation="MTLWSALVVLWTLIAHAGALSGQVGSVLDGDILEVLHQGKAERI
                     RLNGIDCPEKDQAYGERAKQAISDLVVGKEVMLNTYGLDRNGRTLADVLLPDGTNVNY
                     KLVKEGWCWWYRKYAPGNRKLEGLENEAREAKKGLWAEPHPVPPWEWRKKRK"
     gene            complement(1540..1863)
                     /locus_tag="VI359_07375"
     CDS             complement(1540..1863)
                     /locus_tag="VI359_07375"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974109.1"
                     /translation="MDLRRHQRFPVHFHSVLSGPKLSESAGTIVNLSEGGCCVETDSQ
                     VYTGIQLALRLHVPGDETPIRIEQAAVRWTRGHELGVGFITITPPHKERLDQFLQRLK
                     REQQA"
BASE COUNT          445 a          559 c          570 g          475 t
ORIGIN      
        1 ccagcgcacg caacgctcgt ccaacgtggg gtcgcgcgat ttcttcaacg acaacgcctc
       61 gctcggcatg caatatcgat tttgaagggg gaccgccctc aagtggcaag tggtgtgcgg
      121 gagggcaacg tgagtgcgag acggtttggt agtgcgctcg ggcgggacat tcctcaagaa
      181 attgtgcagt caccagaaga agatcaagaa ttctgaatct ggatcaaacg cagtactcta
      241 tcggaacaga taaggaacct gtcgtcgagg taagcaattc ttgtgctggt gagatgagaa
      301 acgaccaagg aagaacattg ataattactg gcgggattcc ttcgcacgga gatcgtgcaa
      361 gcgaacggag acgcgaacct tttcaagtaa tgcttcgcgt gacgcgggct tgcgaagcca
      421 atccgtgacc ccctgtttca caaccgcttc agtgaactga aacgcatctg cgccgctgag
      481 aaccgtgata gggatggttg ggtagcgggc atggaaatgc gcgatcagtt cagtaccctt
      541 gatctgcggc atgtcgaggt cgcagaggag ggcgccgact gtggtggcat ggctgtcacg
      601 ctccatgagg gatatagctt cctgagcatt cgctgcggtc agaacctcat aacctgctcg
      661 cctcaggacc aagcggacgg tatccagaat gtccggatcg tcgtctacta ccaacacttg
      721 acctagactc atgggacttt ccgttaacgg ctcactgtgc caggtcggaa ggtagggtat
      781 tttgcatgaa aaatccagcg cgactgggcg gtgcgatagg agttgcataa gaacaaacga
      841 gatcaagctc taaacataag cagattgacc ctataggggc aaaaccttta gcccgccaat
      901 tgttctcgtt ctggctcctc tccagggagc cgtcacgttt gctcatatcc aacgaacacc
      961 tagcgggcac ggaattctac tttctgtcca agcgatggtt tcctgtagtg gaagggaccg
     1021 gcagggtggc aggattgtcg gcttacttcc ttttcttccg ccattcccac ggaggtacag
     1081 ggtgtggttc tgcccataag cctttcttgg cctctcgcgc ttcattctcg agcccctcaa
     1141 gttttctgtt ccccggcgca tatttccgat accaccaaca ccatccttct ttgaccagtt
     1201 tatagttgac gttggtgcca tccggcagga gtacatcggc aagggtccgc ccgttcctgt
     1261 cgaggccata cgtgttgagc atgacttctt tcccgaccac caagtcggag atggcttgct
     1321 tcgcccgttc gccgtatgcc tgatctttct cgggacagtc gatgccgttg agtcgtatcc
     1381 gttctgcttt gccttggtgg aggacttcga ggatatctcc gtcgagcacg ctgccgacct
     1441 ggccactcaa cgcacctgcg tgagcgatga gcgtccataa gacaacaaga gcactccaca
     1501 aggtcacgca tcccttgttc gccatcaacc ggatctttct caagcctgtt gttctcgctt
     1561 taatcgctga aggaattggt ccaatcgctc cttgtgcggc ggtgtgatgg tgatgaaccc
     1621 cacgcctaat tcgtggccac gggtccaacg caccgctgcc tgttcgatgc ggattggcgt
     1681 ttcgtcgccg ggcacgtgca ggcgcagggc cagctgaatg cctgtgtata cctggctgtc
     1741 ggtctcgaca cagcagccac cctccgacag atttacgatc gtcccggccg attcactgag
     1801 ctttggacca gagagcacac tgtgaaagtg tacaggaaag cgctggtggc ggcggaggtc
     1861 cataagagcc ttcctcccaa tcagtgtgtg aggtgcgctg ggactccccc ttcggcagcg
     1921 ttccctttcg attccgatgt ctcgcaaata tctgacaaaa caaccgacat tgcctgctgc
     1981 cgacgcaagg ctaggctgtc tgtggaaaag tgtcaaggct gacagatgca atcgaagggg
     2041 gtagaatat
//
LOCUS       DATDNW010000310         3608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1071031, whole genome shotgun sequence.
ACCESSION   DATDNW010000310 DATDNW010000000
VERSION     DATDNW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071031"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VI359_07380"
     CDS             complement(<1..311)
                     /locus_tag="VI359_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974110.1"
                     /translation="MVDAQYFGDGLTYLNPSDLKGKLIAIEGTDGVGRSTHIEMLQEW
                     LEVQGYGVITTGWTRSNLMSKTIEMAKEGNILDRWSFTLLYATDFADRLEHQIIPALR
                     SG"
     gene            complement(259..1008)
                     /gene="tmk"
                     /locus_tag="VI359_07385"
     CDS             complement(259..1008)
                     /gene="tmk"
                     /locus_tag="VI359_07385"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY6974111.1"
                     /translation="MSDHPPTRSTPHPYPGKLIIVEGIDGSGKSTQLLLLHKWLESKG
                     HKVFFTEWNSSDLVKDTTKRGKKTKSLTPTTFSLLHATDFASRLYHDILPPLKAGMLV
                     LADRYMYTAFARDVVRGVSPEWVRKLYSFAIRPDMAFYFKVPIEIAISRLLGGTRGQF
                     KYYEAGMDMNLSQDVTESFRIFQSRILAQYDKIVDEFDLITMDATKDIAEQQNEMRRL
                     VEAALIDYKPRRGTHGRRTVFWRRFDVPKSE"
     gene            complement(1005..2558)
                     /locus_tag="VI359_07390"
     CDS             complement(1005..2558)
                     /locus_tag="VI359_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY6974112.1"
                     /translation="MPKIAVIDIGTNSIHMVLAEVLPDFSYRIVDRFKDMTRLGNGAF
                     TTRHLSQDAMTRALDVIRNLVTLARNKGFDRIVGVATSAVREAQNGGDFIDLVAEHAQ
                     LKVRVVSGIEEARLIFLGVKNSIPLTDQPTLAVDVGGGSVELMAGNRDQLLHAKSLKL
                     GAIRLVDQFLQRTPPAVSTLRLLEDTVTAQLTAALNSFKIKRFDSLVATSGMAGNLAE
                     IVHLRRTNHPLVQLNLTKISLKDVREIETDLAQSTIKARLAIPGLDPKRADTLLPATL
                     VLRKLMELTGQDELTLCDKAIREGVIFDFIERHRERLKSEAEIPDLRRRNVVALARRC
                     QAPEVHSLHVASLALRLFDQTQRLHKLSSTERAWLEYAAILHDIGYLINERQHHKHAY
                     YLIVNSQLGGLSVDDIHVVANVARYHRRAAPNQKHEGFELLSTKKKRIVRVLSAILRI
                     ADALDRTHFSVVRTLDVKLGKTITIHLHVTGDAVLETWAAKGRADLFEQVFRRRVKFT
                     VSTEEDDAA"
     gene            2825..>3608
                     /locus_tag="VI359_07395"
     CDS             2825..>3608
                     /locus_tag="VI359_07395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6974113.1"
                     /translation="MPDRTSTSAVVITGASTGIGAACARFLADHGFQVFAGVRDLQAG
                     EVLASGRSLRLIPIQLDVTEDTSIRNAAERVTHHLGDAGLAGLINNAGIAVGSPLEVI
                     PIDQLRRQFEVNVFGQIAVTQAFLPLLRKAHGRIVNMGSIAGRATIPLMGPYSASKHA
                     LEALTDALRLELQQWGIQVSVIEPGAIATPIWEKSIRAAKDLETAADSSAAIGYTELI
                     MRIKETVGKAAERAIPPDAVVRAVVHALTASRPRTRYLVGTDA"
BASE COUNT          759 a          962 c         1037 g          850 t
ORIGIN      
        1 cccgaacgga gggcgggaat gatttgatgc tccaagcgat cggcaaagtc ggtcgcatag
       61 agcagggtga acgaccagcg atcgagaatg ttgccttctt tcgccatttc gatcgtcttg
      121 gacatcagat tggaccgggt ccatccggtc gtaatgacac cataaccctg cacctcaagc
      181 cattcctgca acatctcgat gtgggtggag cgacccacac cgtcggttcc ctcgatggca
      241 atcagcttgc ccttgaggtc actcggattt aggtacgtca aaccgtcgcc aaaatactgt
      301 gcgtcgacca tgggttcccc gtcgtggttt atagtctatc aatgcagcct caacaaggcg
      361 gcgcatctcg ttttgctgct cagcgatatc cttcgtcgca tccatcgtga tgaggtcgaa
      421 ctcatcaacg atcttgtcat actgagccag gatccgagat tggaagatgc ggaagctctc
      481 cgtgacgtcc tgactcaggt tcatgtccat gccggcttcg taatacttga actgcccgcg
      541 tgtccctccg agtaaacggg aaatcgcgat ttcaattggc actttgaaat agaaggccat
      601 gtccgggcga atcgcaaaac tatagagttt ccggacccat tcgggggaga cgccacggac
      661 gacatcgcgg gcgaaggcag tatacatgta ccggtcggcg agcacgagca tgccagcttt
      721 gagcggcgga aggatgtcat gatagagccg gctcgcaaag tcggttgcat gcagcaggct
      781 gaatgttgtc ggggtgaggc tcttggtctt ttttccgcgc ttggtcgtat ctttgaccag
      841 gtccgaggaa ttccattccg tgaagaaaac tttgtgccct ttggactcca gccatttgtg
      901 caataaaagg agttgcgtac tcttgccgga gccgtcgatg ccctcgacga taatcaactt
      961 cccagggtac ggatgtgggg tcgatctggt cggtgggtgg tcgctcatgc tgcgtcgtcc
     1021 tcttctgtcg acaccgtaaa cttcactcgt cgacggaaga cttgctcgaa gagatccgcg
     1081 cgccctttcg cggcccatgt ttctaacacc gcgtcgcccg tgacatgcag gtggatcgtg
     1141 atcgtcttgc cgagcttgac gtcaagtgtg cggacgacag agaagtgtgt ccgatcaagg
     1201 gcgtctgcaa ttcggaggat tgctgacagg acacgcacga tccgtttttt cttagtagac
     1261 aacaactcga acccttcgtg tttctggttt ggagcagcgc gacggtgata ccgcgcaacg
     1321 ttggcgacga catgaatgtc gtcaacggag agtcctccca actggctgtt gacgatcaag
     1381 tagtacgcat gtttatggtg ttgccgttca ttgatgagat agccgatgtc gtgcaggatc
     1441 gcggcgtact cgagccacgc acgttctgtg gagctcagtt tatggaggcg ttgtgtttga
     1501 tcaaaaagac gcaacgcaag gctcgcgaca tgcaagctat gcacttcagg agcttggcac
     1561 cggcgggcca gcgcgacgac gtttcgccga cgaagatcgg gaatttcggc ttccgatttg
     1621 agccgttcgc ggtgccgctc gatgaagtcg aaaatgaccc cttcgcgaat cgccttatcg
     1681 cagagagtca gttcatcttg cccggtcagt tccatgagct tgcgcaagac cagtgtggct
     1741 ggtaacaggg tgtcggcccg cttcgggtcg aggccgggaa tagccagacg cgctttgatg
     1801 gtcgactggg cgaggtcggt ttcaatttcg cggacatctt tgagtgagat tttcgtcagg
     1861 tttaattgga ccaaggggtg gttcgtacgg cgtaaatgca caatttcggc gaggttgccc
     1921 gccattcccg aggttgcgac caacgaatcg aagcgtttta ttttgaacga attcaaggcg
     1981 gcggtcagct gtgcggtcac ggtatcttcg agcaaccgga gtgtgctgac ggcgggaggc
     2041 gttcgttgaa ggaactgatc gaccaggcgg attgctccga gcttcaggct ttttgcgtgg
     2101 aggagttggt cccggttgcc cgccatgagt tccacggagc ctcctccgac atcgacggcg
     2161 agagtcggct gatctgtcag ggggatactg ttcttcactc cgaggaagat caaacgcgct
     2221 tcttcaattc cgctgacgac tcggaccttg agctgagcgt gttctgccac cagatcgatg
     2281 aaatcgcctc cgttttgggc ttcccgtacg gcgctggtcg ccactcccac aattcgatcg
     2341 aaccccttgt ttcgagccag cgtgaccaaa ttgcggatca catccaatgc tcgggtcatg
     2401 gcgtcctggg agaggtgccg ggttgtgaag gctccgttgc ccaaccgggt catatctttg
     2461 aaccggtcca caattcgata gctgaagtca ggcaggacct cggccaggac catgtggatg
     2521 gaatttgtcc cgatatcgat gaccgcaatc ttcggcatgg caggtaatag tatcaataat
     2581 ttacacggta ttgcacgggg atttatgagc ctcaaggaac agatacccat tccttgttat
     2641 ggcgcagacg aggtagtcaa atccttgtcc gaatggatga ggaccatagt taaacaacca
     2701 ggttctgtca aggcgtgtat ttgttaagtt taagttacgc aattatctcc tcctctgccc
     2761 tttgcgctcg tgctaggcta ctccaatttc gatgagaacg ggctctcacg tttctaacat
     2821 atctatgcca gaccgcacat ccacgtcagc tgtcgtgatt accggtgcct cgaccggtat
     2881 tggcgctgcg tgtgcccggt ttttggctga tcatggtttc caggtgttcg ccggtgtgcg
     2941 agatcttcag gccggagagg tgttagccag cggccggtcc ctacgattga tcccgatcca
     3001 actcgatgtc accgaggaca cctccattcg aaacgcagcg gagcgagtca cccatcatct
     3061 tggcgacgcc ggattggccg gtctgatcaa caatgccggt atcgcagtcg ggagccccct
     3121 cgaagtgatt ccaattgatc aattacgacg gcagttcgaa gtcaacgtat ttggacagat
     3181 tgccgtcact caggcgttcc ttccgttact tcgaaaggca cacggccgca tcgtgaacat
     3241 gggttcgatc gcgggtcgcg ccacgattcc gctgatggga ccctattctg cgtcgaagca
     3301 tgccttagaa gccctcacgg acgcgctacg tctcgagtta cagcagtggg gcatacaagt
     3361 gtccgtcatc gaaccgggag ccatagcgac gccaatctgg gaaaagtcca tcagggctgc
     3421 gaaggatctt gagacggcag ccgattcgtc cgccgcaatc ggctacacag agctgatcat
     3481 gcggatcaaa gaaacggtcg ggaaggctgc agaacgggcg attccacctg atgccgtcgt
     3541 gcgagctgtc gtccatgcgt tgacggcatc acgcccgcgc acacgttatc tcgtgggaac
     3601 cgatgcga
//
LOCUS       DATDNW010000311         6179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_42555,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000311 DATDNW010000000
VERSION     DATDNW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42555"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..290)
                     /gene="rpmE"
                     /locus_tag="VI359_07400"
     CDS             complement(84..290)
                     /gene="rpmE"
                     /locus_tag="VI359_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY6974114.1"
                     /translation="MKKGIHPLYREATVHCACGNKFKTRSTVGDINVDICSNCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKGK"
     gene            complement(389..1636)
                     /gene="rho"
                     /locus_tag="VI359_07405"
     CDS             complement(389..1636)
                     /gene="rho"
                     /locus_tag="VI359_07405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY6974115.1"
                     /translation="MHLAELKQKTIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERIILEFDREEYCTRVMD
                     LITPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRIFPAIDISQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKVQGTKTNQEFLQSMNR"
     gene            2175..2579
                     /locus_tag="VI359_07410"
     CDS             2175..2579
                     /locus_tag="VI359_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY6974116.1"
                     /translation="MAHGEEYERTFTVSEANHLIPQLHTRFISIQRAKAILLQTKQEI
                     QKASAQARYGGGSSVGHLYISGLQQVGTNLQAIHELGVLVKDVDIGLCDFPHLRDGRV
                     VYLCWKLGEDEVRWWHETTTGYKDRCPLEERR"
     gene            2622..2900
                     /locus_tag="VI359_07415"
     CDS             2622..2900
                     /locus_tag="VI359_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY6974117.1"
                     /translation="MKFVILGFDGPDGEAKRKIHRPAHLDNLAPLSAQGRVVLAGPLT
                     DKTGSLIVIEADSLEEAQLFAREDPYTVHGVFERVEVHPFLQVFPKPD"
     gene            3046..4314
                     /locus_tag="VI359_07420"
     CDS             3046..4314
                     /locus_tag="VI359_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974118.1"
                     /translation="MITQRFLHIALIPLSIAFSTIPSLAASTKIVVAEGQYAMADGDT
                     LGAAEEKVLQRAQRRAVEEAGIYLESTFHDTEKESGGRTSQTSSLEIRTIAAAITQTE
                     ILESRRSFENERPVFFIRIRATVNLDSLAEAVRRMKSDEQMARHFRQLQQENHQLRAQ
                     LQDLQSQPSGVRTLVIEPTGRGEIYYRARTLLNQALQTNDLNDKIALTSEALDLDGRQ
                     VEPLIVRGQTFLRLVSMAFSQHSKTADYEPYLYKAKADFDRAVAIDGKNAWAWLGQGD
                     VYSWLKHTEDAASSYEKVLELDPFFDAARQRLIALYTTQARRQAQAKQWQQALSTLKK
                     LLSAQTSESWIPYQKEAYLLRSEIYVKLNQPEQAIDDLSTVLRVDPTNADALLTRAKL
                     YRERLQGRLAKDDLERACVLGSIPACEQLP"
     gene            4664..5299
                     /locus_tag="VI359_07425"
     CDS             4664..5299
                     /locus_tag="VI359_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HEY6974119.1"
                     /translation="MSQYRVLPGPEHFLPPAAASMGIRLPNPGEAHINGVIVPEEKAY
                     EEAARQFLMAKVPTIFPGPLVLWAWNEKAAKKATAIRHLFNTLKECVQPGQKPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDACMFVGVHCHQANLSLKIIRGGTSCYT
                     IAMCAQAGHEDAMLSFRDASVEKIMRLADTVKRLKGTVKPRLGTAASTASN"
     gene            5340..>6179
                     /locus_tag="VI359_07430"
     CDS             5340..>6179
                     /locus_tag="VI359_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HEY6974120.1"
                     /translation="MADTHSVIGTQNKKGQTYTDTWKMMNEAPRTPSFFTGSEVIKEA
                     IRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEVWAAVERSRKHSIHWL
                     NGLI"
BASE COUNT         1413 a         1707 c         1623 g         1436 t
ORIGIN      
        1 ctctccgtcc cgactccttg ctcacaaaaa aagagaccct gctccatcgc gaagcagggt
       61 ctcttctatg gtgctcagag ttgctacttg ccctttttcg cgtacttctt cttgaaccgt
      121 tcgacacgac cttcggtatc gacgattttc tgcgttccgg taaagaacgg atggcaattc
      181 gaacaaatgt cgacattgat atcgcccacc gttgaacgcg tcttgaactt gttgccacag
      241 gcgcaatgga ccgtggcttc ccggtagagc gggtgaatcc cttttttcat aaccatcaac
      301 tccttacatc acggttgcat tgcggcacgg cccccgtgca ggggcatgac actgtatctc
      361 gaccggcggt tgagatacaa gcggcccgct atcggttcat cgactgcaga aattcctggt
      421 tggtcttggt cccctgaacc ttgtccatca ggaactccat cgcctccatg gttcccagcg
      481 ggctgagtac cttgcggaga atccacatct tgttgaggcg atccttgtcc accaacagct
      541 cttctttcct tgtcccagac tgactgatat cgatcgccgg gaagatccgc ttgtcggcca
      601 gacggcggtc aagatgcact tccatattgc cggtgccctt gaactcttca aagatcacat
      661 cgtccatgcg actgccggta tccaccaggg cggtggccat gatcgtcaga ctgcccccgt
      721 tttcgatatt ccgtgctgcg ccaaagaacc gcttcggtct ctgcagggcg ttcgaatcga
      781 gaccgccgga cagcaccttg ccgctgggag gcgcgatggt gttgtatgca cgcgcgagtc
      841 gtgtaatgct gtccagcaga atcacgacgt ccttcttgtg ctcaaccagc cgcttggcct
      901 tttcaagaac catttcggcg acttgggcat ggcgctgggc cggctcgtcg aatgtcgagc
      961 taatcacttc cgccttcacc tgccgctgcc agtcggtcac ttcctcgggc cgctcgtcga
     1021 tcaggagtac gatcaaggtg acttccttgt gattcttgag gatggcccgc gcgatggctt
     1081 ggagcagcat agttttgccg gttcgaggcg cggctacgat gagtccccgc tgccctttcc
     1141 caatcggcgt gatcagatcc ataacccgcg tacaatattc ttcgcggtcg aattcgagaa
     1201 tgattcgttc ctcaggatag aggggggtca ggttatcgaa aagaatcttg tcgcgggcga
     1261 cttccggatc ttcatagttg accttttcga ccttgagcag cgcgaaataa cgttcgcttt
     1321 ctttcggtgg tctgatctgt cctgaaacaa tgtctcccgt acgaagattg aaccggcgga
     1381 tctgcgaggg ggagatgtaa atgtcgtccg ggccgggaag gtagttggag tcgggggccc
     1441 gtaagaatcc gaatccatcg ggcagggttt cgagtacgcc ctcgccaaag accacgccgt
     1501 ttttttccgt ctgcgcttgc aggatcgcga agatcagctc ctgcttacga aggttcgctg
     1561 ccccctcgat cttgaggtcg cgtgcgacgt cgtttaaatc ggcgatggtt ttctgtttca
     1621 actcggcaag atgcataacc tcccccttgg tttgtggcat acgaatccct tcaagctcta
     1681 ggcttactga tcacaaggca ctcccgaatc ggacaatcat gggccaaatg ttcaacaact
     1741 gggtggtaat gaattggcgt tggtccgtgt gctcgttttt gaaatggaga gggttaaatt
     1801 ctgaagggcc tatccgtttg ggtccgcttt cgtcacgctt gagctactta cgggaactgg
     1861 aaacgtgtcc actatttcac aacgatgctg aattctctcg tctctatcat gcgaccaatg
     1921 tagctttttt cgagacaggc cccggaagct ttatcagcgg aggaatgttt gagtcgaacg
     1981 ataacgttgg tgagatgata cctcgcagga atctccttgt caatatatgt tatggttttc
     2041 ccaaaaactg cgtcacgcgt cagaggcgga acctattggc gctgtgcctc gcacgcatgc
     2101 ttcttcggca taaccccggc tcggttgcag cagagaattg ggtgtgctaa aggaaggaca
     2161 cggcgaggca cccagtggcg catggcgaag aatacgaacg cacattcacc gtttccgaag
     2221 ccaatcatct gattccgcag cttcacacgc ggtttatctc gatccaacga gccaaagcga
     2281 tcctccttca gacgaagcag gagattcaaa aagccagtgc ccaagctcga tatggcggtg
     2341 gcagctcggt cggtcacctg tacatctcag gcttgcagca agtcggcact aacctccaag
     2401 cgattcacga actcggcgtg ctggtcaagg atgtggatat cgggctgtgc gattttcccc
     2461 atctccggga cggacgggtc gtctatctct gttggaagct cggcgaagat gaggtccgat
     2521 ggtggcacga aaccacgact ggctataagg atcggtgccc gctcgaagaa cgccggtgaa
     2581 actgacgttt gctcgtacac tgcttgctag cacctatacc catgaaattt gtgatcctgg
     2641 gattcgatgg tcctgacggg gaagccaaac gaaaaatcca tcggcccgct catctcgaca
     2701 atctcgcccc actgagcgcg caagggcgcg tggtgctggc aggccccctg accgataaaa
     2761 ccgggagtct catcgtcatc gaagccgact cgttggagga agcccaactg tttgcccgcg
     2821 aagatcccta caccgtgcat ggtgtgtttg agcgtgtcga agtgcatccc ttcctgcaag
     2881 tcttcccgaa gcccgattga caccttcctt aattctgaca cccactcaat ctcgtaactc
     2941 attgaatcat agatagttgc tcggaatgtt accggtctaa tctttagccg cttcctcccc
     3001 tttcgatgca ttgctggggt atcccttgag cggtatattt gaagcatgat tacgcagcga
     3061 ttcctgcaca ttgcactcat tcccctgtcc attgcctttt cgacaatccc gagccttgcc
     3121 gcctctacaa aaattgtggt cgcagaaggt cagtatgcga tggcggatgg cgatactctc
     3181 ggagccgcag aagaaaaagt tctgcaacga gcgcagcgcc gagctgtgga agaagcaggg
     3241 atttatttag aatcgacctt ccacgataca gaaaaagaat cgggcgggcg aacttcacag
     3301 actagttcct tggagatccg gaccattgcc gctgcaatta ctcaaaccga gattcttgaa
     3361 tctcggcggt cctttgaaaa cgagcgtcct gtgtttttca tccgcatcag ggctacagtc
     3421 aatctcgaca gtctcgcaga ggccgtcagg cgaatgaaat ccgacgaaca aatggcgcgc
     3481 catttccgcc agctccaaca agagaatcat caactgcgcg cgcagcttca ggatttgcaa
     3541 tcgcagccct caggggtccg tacattggtg atcgaaccaa ccggacgagg agaaatctac
     3601 taccgtgccc gcaccctctt gaatcaggcc cttcaaacca acgacctgaa cgacaaaatc
     3661 gccctcacct cggaagctct tgatctcgac ggacgacagg tcgagccgct gatcgtaaga
     3721 gggcaaacgt ttcttcgact ggtttccatg gcattttctc aacatagcaa gacagccgac
     3781 tacgaaccgt atctctacaa agcgaaagcc gattttgatc gtgcagtggc gattgacggc
     3841 aagaacgcgt gggcctggtt ggggcaaggt gatgtctact cgtggctcaa gcatactgaa
     3901 gatgccgcct cttcctatga aaaggttctc gagctggatc cattttttga cgccgcgcgc
     3961 caacgactga tcgcactcta tacgactcaa gcacgaagac aggctcaggc aaaacaatgg
     4021 caacaggctc tgagtacact gaagaaactc ctgtctgcgc agacgtcgga gagttggatt
     4081 ccctatcaaa aggaagccta ccttcttcgt agtgaaatct atgtgaagtt aaatcagccg
     4141 gaacaggcta tcgacgatct ttccaccgtg cttcgagtgg atcccacaaa cgcagatgcc
     4201 ctcttaacaa gagcaaaatt gtaccgagaa cggcttcaag gtcgactggc caaggatgat
     4261 cttgaacggg cctgtgttct aggttcaatc cctgcgtgtg aacagttgcc ttagatgaaa
     4321 tggctttcta acttgttgaa ttcacaaatg tatgttctga gtgttaccac aaatttttct
     4381 gcggactcag gtagtgcagt accagtccgc tggtttgaca atgtcaaaga tggcctccta
     4441 taatgcgcgc tccgatctcg ctttgggctc tcacgttcgc atcgtctacg ttgggacaaa
     4501 aggcctaagc tggtccggtc cgatagcccg aaatttccct acggtgggcc ttctgtcagg
     4561 ttgcagagag acttgaaaaa gcttagagtg aacaaggttc cacgtattgc cagacgagtc
     4621 ggtcctgtcc ttctaattcc ttagcgtcgg agagtaaatc cgcatgagtc agtatcgtgt
     4681 actcccaggc ccagaacatt ttttacctcc cgccgcggca agcatgggaa tccgcctgcc
     4741 aaatcccgga gaggctcata tcaatggcgt aatcgtgccg gaagagaaag cttacgagga
     4801 agcggccagg caattcctaa tggcaaaagt tcccacgatt ttcccaggac ctctcgtcct
     4861 gtgggcatgg aacgaaaagg ccgcgaagaa ggcgaccgcc atccgccatc tgttcaatac
     4921 gctcaaagaa tgcgtgcagc ctggacagaa accgatgctg attccgatgc cggattatcg
     4981 gccgaagtac cccaagatca atcctgaagt ggaaatcaac ccgaaccatc cgaatctcac
     5041 catttggcac aacaagatcg acgcctgcat gtttgtcggc gtccactgcc accaagccaa
     5101 cttgtcgttg aagattattc gcggcggcac gtcctgctac accatcgcca tgtgcgcgca
     5161 agcaggccat gaagatgcca tgctgtcgtt ccgtgacgca tccgtcgaaa aaattatgcg
     5221 attagctgac actgtgaaac gattgaaggg taccgtcaaa ccacggctgg gaacagccgc
     5281 gagcaccgcc tcaaactagg cgtttgaggg acaggggaga gggaaaggag gctggggcca
     5341 tggcagacac acactcagtc atcggcacgc aaaataagaa aggccagacg tacaccgaca
     5401 catggaagat gatgaatgag gccccgcgca cgccttcctt tttcaccgga agcgaagtga
     5461 tcaaggaagc cattcgtcgc gccagctgtg atgtcatgat cgcctatccg atcacgccgc
     5521 aaagcgaagc cgccgccttg atcggcgaac ttttcgccga aggatacatt ggcgactact
     5581 tccgtggtga gagtgaattc gccgtcatgt cgcagtgtgc cggagccgcg ttcggtgggg
     5641 cccgcgtgtt tacgacaact gcgggaccag gcacgatgcg tgcgatggaa aacttcccca
     5701 tgtgggcggg tgcgcggtta ccgatccaaa tgatcgtgac ctgccgtggc atcaactcgc
     5761 cgctgtcgat tcagccggac acactcgaga tcgcctatct gttgaacacc ggcatgcttg
     5821 tctggcatgc ggagaccgct caagacttct ttgattggat tctcaagggc tacatggtgt
     5881 ctgaggaacc ggacgtgcac ttgccgctcg cgctctgctg cgatgggttc ttcgtcacgc
     5941 acacgaaaga cgtcgtgaat ctgacaccag ccgacatgtg tttgccgccg tatgacccct
     6001 atcgttcacc agttccctgt atggatatgg agtgtccacc ggttcggatg atgcgcgacc
     6061 cgttcgtcat gaagagcaat tacatcagct acgccaccca tgcgagttgg caacaggaag
     6121 tctgggctgc tgtcgaacgg tcgaggaaac attcgatcca ttggctgaac ggattaatc
//
LOCUS       DATDNW010000312         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_250795, whole genome shotgun sequence.
ACCESSION   DATDNW010000312 DATDNW010000000
VERSION     DATDNW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250795"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2528
                     /locus_tag="VI359_07435"
     CDS             <1..>2528
                     /locus_tag="VI359_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY6974121.1"
                     /translation="FMRQVFIARDVLNEGQFERKLYVIRKRVEGAVRESAIRGREYFY
                     IPSLSANTIVYKGLLLPHQMAAYYQDLNDASLTSALALVHSRFSTNTFPTWPLAHPYR
                     YICHNGEINTLKGNVNWMRARQGRLNSDLFGEDLAKLYPIVYEQQSDSACLDNAVEFL
                     TMGGRSLPHAMMMLIPEPWVANPQMDLDRRGFYEYHAAMQEPWDGPAAVCFTDGKFIG
                     ATLDRNGLRPCRYQVTTDGLVVLASEAGVLPMDIQKIRQKGRLMPGRMFLVDTVQGRI
                     IDDEEVKAEIVSRKPYRSWVTQYRISLDELPEPINVPQPDHPTIRQRQQAFGYTVEEL
                     KMVVTPMVVEGQEAVSSMGTDTPLAVLSDRPQLLFKYFKQLFAQVTNPPIDPIREELV
                     MSLTTSIGPKPNLMDEHPESCRRIRVKQPILTNADLQKIREIADPHFKSKTLKMLFRV
                     AEGPEGLGAAVDELCRQASQAIKEGFKFLILSDRGVNEEWAPIPSLLGVAAVHHHLVR
                     ECTRTEVGLTLETGEPRDVHHFACLIGYGAGTVNPYLVFESLVDMERDGYLPEGLDAQ
                     TAEGKYIKAINKGLLKIFSKMGISTVQSYCGAQIFEAIGLNHNVINRYFTGTPSRVEG
                     IGIREIGEESLRRHRLAYEPAPIRQLDFGGEIHYRIQGEHHNWNPDTIYKLQHATKAN
                     DPKTFAEFSQLVNDESKRRSNLRGLLDFKFLPAPIPIDEVEPAKEIVKRFTTGAMSYG
                     SISKEAHETLAIAMNRLGAKSNTGEGGEDPERFKPLPNGDSKNSYIKQVASARFGVTS
                     HYLVNAKELQIKMAQGAKPGEGGQLPGHKVDEIIARLRYATPGV"
BASE COUNT          563 a          741 c          757 g          467 t
ORIGIN      
        1 ttcatgcggc aggtgttcat cgcgagggac gtgctcaatg aagggcagtt cgagcgtaaa
       61 ctctatgtca tccgaaagcg ggttgaaggc gccgttcgag aatcggccat tcgtggccgg
      121 gaatattttt acattccgag cctctcggcc aatacgatcg tctataaggg cctgctgttg
      181 ccgcaccaga tggcggccta ctaccaggac ctgaacgatg cgagtctgac gagcgccctc
      241 gcgctcgttc actcgcgctt cagcaccaac acgttcccca cctggccgct cgctcatccc
      301 tatcgttata tctgccacaa cggcgagatc aatacgctca agggaaacgt caattggatg
      361 agagcgcggc agggtcgttt gaattcggat ttgttcgggg aggacctggc caagctgtat
      421 ccgattgtct atgaacagca aagcgactcg gcctgtctgg acaatgcggt cgagttcctg
      481 acgatgggtg gccgctcgct cccacatgcg atgatgatgt taattcccga accctgggtc
      541 gcgaatccgc agatggacct cgatcgccgt ggcttctacg aataccacgc ggccatgcag
      601 gaaccctggg acggtcctgc cgcagtctgt ttcaccgacg gcaaattcat cggggcgacg
      661 ttggaccgca acggcctgcg tccctgccgc tatcaagtga cgacggacgg tctcgtggtg
      721 ctcgcgtcgg aagcgggcgt gctcccgatg gacattcaga aaatccggca gaagggccgg
      781 ttgatgcccg gacggatgtt tttggtggat acggtccagg gccgcatcat cgacgatgag
      841 gaagtgaagg ccgagatcgt cagccggaaa ccctaccggt cctgggtcac gcagtatcgg
      901 atctcgctcg acgaactgcc ggagccgatc aacgtgccgc agcctgacca tccgacgatc
      961 cgtcagcggc aacaggcctt cggctacacc gtagaagaac tgaaaatggt cgtcaccccc
     1021 atggtggtgg agggccaaga agccgtgtct tcaatgggca ccgatacgcc attggccgtg
     1081 ttgtcagacc gcccgcagct gctcttcaag tatttcaagc agctcttcgc ccaagtgacg
     1141 aatccgccga tcgatccgat ccgcgaagaa ctcgtcatgt cgctcacgac cagcatcggg
     1201 cccaagccga atctgatgga cgagcatccg gagtcctgcc gccggatcag agtgaagcag
     1261 ccgatcctga ccaatgccga tctccagaag attcgggaga tcgccgatcc tcatttcaag
     1321 agcaagacac tcaagatgct cttccgcgtc gccgaagggc cggagggatt gggcgccgcc
     1381 gtcgacgaac tctgtcggca agcctcacag gcgatcaagg aaggcttcaa gtttctgatt
     1441 ctgagcgatc gcggtgtgaa cgaagagtgg gcgccgattc caagcctgct gggcgtggcg
     1501 gcggtgcatc atcacctcgt ccgcgagtgc acgagaacgg aggtcgggct tacgctggaa
     1561 accggcgaac cgcgcgacgt ccatcacttc gcctgtttga tcggctacgg agccggcact
     1621 gtcaacccgt atctggtctt tgagtcgctc gtcgacatgg agcgggacgg ctatctgccg
     1681 gaagggttgg atgcccagac cgccgaaggc aagtacatca aggcgatcaa taaggggctg
     1741 ctcaagatct tctcaaagat ggggatttcc accgtccagt cctattgtgg cgcccagatc
     1801 ttcgaagcga tcggcctcaa ccataacgtg atcaaccggt acttcaccgg gacgccgtcg
     1861 cgggttgaag ggatcggcat ccgcgagatc ggcgaagaaa gcctccgcag gcatcggcta
     1921 gcctatgagc cggcgccgat cagacaactc gatttcggcg gcgagattca ttatcgcatc
     1981 cagggcgaac accacaactg gaatccggac acgatctata aattgcagca cgcgacgaag
     2041 gcgaacgatc ccaaaacgtt cgcagagttc tcgcagctcg tcaacgatga gagcaagcga
     2101 cggtcaaacc tacgcggcct gctcgacttc aagtttctgc cggcgccgat ccccatcgat
     2161 gaggttgagc cggccaagga gatcgtcaaa cggttcacga ccggagccat gtcgtacggc
     2221 tcgatcagca aggaagcaca cgagacgctc gcgattgcca tgaaccgcct gggcgccaag
     2281 agcaataccg gcgagggtgg ggaagacccc gagcgattca aacccttgcc gaacggcgat
     2341 tcgaagaaca gctatatcaa acaggtggcg tcggcacggt tcggcgtgac gagccactat
     2401 ctggtcaatg cgaaggaact ccagatcaag atggcgcaag gcgccaagcc gggggaaggc
     2461 ggccagctgc cggggcacaa ggtggatgaa atcattgcgc gactgcgcta tgccacaccg
     2521 ggtgtcca
//
LOCUS       DATDNW010000313         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_868757, whole genome shotgun sequence.
ACCESSION   DATDNW010000313 DATDNW010000000
VERSION     DATDNW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868757"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>2117)
                     /locus_tag="VI359_07440"
     CDS             complement(138..>2117)
                     /locus_tag="VI359_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY6974122.1"
                     /translation="GCQMYIETKDTEVIRITSPLSWKNKWGDRAETSKGHGGLCVRGR
                     FGFEYLDSPARLKQPLVRKGNQLVETPWLETMHHVVERFSEIRKRHGSDAIAGLITAR
                     CTNEELYLFQKLFRAGFRTNNLDSSARYGHLNFIHASRQALGIGRSPNDWEDLTKAKA
                     VLLIGSNITETNPLTAVRIKEAIRVYKAQVVTIDSAITNMGKLASHPFVIKAGTEGLV
                     IDGLVKAAIELDLVDEEVIKQHPQAFAALKAALAHVSLEQIAAQTGMTIQDLKDAATV
                     FAEAPRSIIICAEGIVRRTDGHQNVLKLIDLAWITGKLGRPGCGVNTLTEEPNEQGAV
                     DMGVAAEFLPGQARFDDQAARDRFAKAWDVTLPAAGSGTHLVEILNRCKSGQIRALYV
                     VGENPLATLPASMEVRAALDRLEFLVVQDPFLTETARLAHVVLPACTYVEKDGTFTNL
                     EGRVLRVRQAMDPIGESLPDWHIMTALANGLGCQWEYESTNDIQAEIMKLLPGYYNLG
                     QPRKVTPNADHYLANGYTSTVANRYRSSTVRPAGADQRPFALLMGQLLYHSGKMSSEA
                     PGLIKVAPNTGRLRMSPQDMERLGLIDGAKVRVTSDRGSLQLGVQPDQSVAPHTCFFP
                     EHFNEPPVKDLMSIQLDPVTNVPSFKLTRVTIEKA"
BASE COUNT          417 a          594 c          618 g          488 t
ORIGIN      
        1 gaggatactg gaacgtgatg ggcttgtgga acatgtgctt gaacgtcact ttcatcgcgt
       61 cccagatctc atagaacagc gcggcctgca gaactttttt ggtgagcgtg acgacactca
      121 tcagcttctc cgtgccccta agccttttcg atcgtgacgc gcgtgagctt gaacgaagga
      181 acgttagtca cggggtcgag ctggatcgac atgagatcct tcaccggtgg ttcattgaag
      241 tgctccggga aaaaacaggt gtggggcgcc accgactgat cgggttgcac gcccaactgg
      301 agcgacccgc gatctgaggt gacgcggacc ttggctccat cgatcagacc gagccgttcc
      361 atgtcttgcg gactcatccg cagacgaccc gtattggggg cgaccttgat gagccccggt
      421 gcttcgctcg acatcttgcc cgagtgatac aacagttgcc ccatcagcaa tgcaaacggc
      481 cgctggtccg caccagcggg tcgaacggtg ctactgcgat aacggtttgc caccgtcgag
      541 gtgtagccgt tggcgaggta gtgatccgcg ttaggcgtga ccttccgggg ctgtccaagg
      601 ttgtagtagc cgggcagcaa tttcatgatc tcggcttgaa tgtcattcgt ggattcatac
      661 tcccactggc acccgagacc attcgcgaga gccgtcataa tatgccaatc tgggagactt
      721 tccccgatgg gatccatcgc ttggcgaaca cgcaagacgc gcccctccaa gttggtgaag
      781 gtgccgtctt tctcgacata cgtacaggcc ggtaaaacga cgtgtgcgag gcgggcagtt
      841 tccgtgagga acggatcctg aacgaccagg aattccaggc gatccagcgc cgctcgaacc
      901 tccattgagg ccgggagcgt cgcgagcgga ttttctccga ccacatagag ggctcgaatc
      961 tgtccgcttt tgcagcgatt gagaatttcc acgagatggg tcccgcttcc cgcagctgga
     1021 agcgtgacgt cccacgcttt ggcaaatcga tcacgtgcgg cttggtcgtc gaatcgggcc
     1081 tggcccggga gaaactccgc tgccacaccc atgtcgaccg cgccttgttc gttgggttcc
     1141 tcggtcaatg tattgacgcc gcagccggga cgccccagct ttcctgtgat ccaagcgagg
     1201 tcgattaatt tgagcacatt ctgatgacca tcggttcggc ggacaatacc ttccgcacag
     1261 atgataatgg accgcggagc ctcggcgaac acggtggccg cgtctttcaa gtcctggatc
     1321 gtcatgccgg tttgtgctgc aatctgttcc aaggacacgt gtgccagagc ggctttcaaa
     1381 gcggcaaacg cctgcgggtg ttgcttgatg acttcttcgt ctaccagatc cagttcaatt
     1441 gcggctttga cgagtccgtc gatgaccagc ccttcggttc ccgccttgat cacgaatgga
     1501 tgtgaggcaa gcttgcccat gttcgtgatc gcggaatcaa tcgtgacaac ctgggccttg
     1561 tagacacgga tggcttcctt gatccgcacg gccgtgagcg gattggtttc cgtaatgttc
     1621 gaccctatca gcaagacggc cttcgccttg gtcaaatctt cccagtcgtt cggtgagcgt
     1681 ccgattccca gcgcttgacg ggaggcatgg atgaaattca agtggccgta acgcgcactg
     1741 ctgtcaaggt tgtttgtgcg gaatccagct cggaagagtt tctggaacag gtacaactct
     1801 tcgtttgtgc agcgagcagt aatcaagcca gcgatcgcgt cggaaccgtg tcgcttgcgg
     1861 atctcggaga atcgttcgac gacgtggtgc atcgtttcga gccacggagt ttcgacgagc
     1921 tgattcccct tgcggaccag cggttgtttc aagcgcgcgg ggctatcgag gtattcgaat
     1981 ccgaatcgtc ctcggacgca gagcccacca tggcctttcg aggtctcggc gcgatcaccc
     2041 catttatttt tccacgacag cggtgacgtg atgcgaatga cttcggtgtc tttggtttca
     2101 atgtacatct ggcagcc
//
LOCUS       DATDNW010000314         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1142636, whole genome shotgun sequence.
ACCESSION   DATDNW010000314 DATDNW010000000
VERSION     DATDNW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142636"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VI359_07445"
     CDS             <1..286
                     /locus_tag="VI359_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974123.1"
                     /translation="LATGAGAHGRVLLGLAVFGGMLAASVIAIFLIPVSFYVVESLSH
                     RGAPHARPTPGPPVPGTPKGPGDGHPSQPVLQPALSPTHARESGSYGGGH"
     gene            288..1877
                     /locus_tag="VI359_07450"
     CDS             288..1877
                     /locus_tag="VI359_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6974124.1"
                     /translation="MGAKREARDGRDMREKRDRNERAGWAARLYIMVVFTGVAFMQGA
                     CMMGPDYTRPDTPKADAWRLTPATAESIANMPWWELLKDAELQKLIRAALQENLDLQI
                     AAANIEEFQAQLMIAKFDLVPSLNYTGSVFGFRNTNDNVVPLAGGALPTTTPGKGGGL
                     SLSHEAAEVGLKWEVDLWGRIRRSIESARAQLLSKEENQRAVILGLVSTVAQSYFDLR
                     GLDYEVEITKRTLKAWDESVRLSELRYKNGVIPKLDLDRFQAERAGTAAQLADLERQV
                     VQTENRLNILLGRRPAEIARGLHLTNQSMPPAVPPGLPSDLLQRRPDILKVEQDLAAT
                     NANIGVAQATRFPQLALSGGIGGAGLQFQGSSFGPFATFKGSASLTGPLFNASALGYQ
                     VKATEAQTQAAVAQYRKTIITAFQEVEDALIAVQKTGEQRIAQEQQVAALESAYKLAD
                     FRYQGGRASYLDVLTAQRSLFDAELGLARTRRAQLISVVQLYKTLGGGWSPEMAGKKT
                     QTAGGRGEAAESTEAIPVAIR"
     gene            1893..2690
                     /locus_tag="VI359_07455"
     CDS             1893..2690
                     /locus_tag="VI359_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEY6974125.1"
                     /translation="MWQAMFIQTSRSRMHAAVALVATLIAMGCASTVVKSEYESAKLR
                     LQRPSQIFVYNFAVTRQQVKENQGLFQGTVNEFKGETTFEHEGEIAEEVQTVAADELV
                     ERIQNLGLPAKKVSGTTPLPKGSLGITGQFLDVDEGNRARRLVIGFGSGQSKVDIRIQ
                     LYGFGLDPSRTEPEIAPTKLLEFETHADSGSMPGAAVTAGAGAAAGAGAGAVVGVNAA
                     IGGAKSYRSSMGVMTAKSAQQAVDLMSEFFGRQGWIERDKVKYADRP"
     gene            2799..>3273
                     /locus_tag="VI359_07460"
     CDS             2799..>3273
                     /locus_tag="VI359_07460"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEY6974126.1"
                     /translation="MRVLLWIGVFVFAVTLTGCASTYQAEPKQSGFLGDYSLLKKGGE
                     GEAALVYRKYDNDRTKWRSYSEILLEPVQIWKGEKTELNPDDAEYLAKIVWSRLDEEL
                     RKDYKMTSSPGVGVLRIQTAVTEAGTNMPVLDLVTTLYPGTRLISEGKRWASGTES"
BASE COUNT          747 a          856 c         1019 g          651 t
ORIGIN      
        1 cctcgcaacg ggagcgggag cgcatggacg agtcctcctc ggccttgccg tcttcggcgg
       61 catgctggcc gcgagcgtca tcgcgatctt cctcatcccg gtctcgttct atgtggtgga
      121 gagcctctcg catcgcggag cgcctcacgc aagaccgacg ccgggcccgc ctgttccagg
      181 tacgcccaag ggaccaggtg acgggcaccc atcccaacca gtcctgcaac cggcactgtc
      241 accgacccac gcacgcgaga gcggaagcta cggaggaggg cactgagatg ggagcaaagc
      301 gagaagcgcg ggacgggcga gatatgcgtg aaaagcgtga taggaacgag agagcgggat
      361 gggctgctcg cctctatata atggtggtgt tcacgggtgt ggcgtttatg caaggggcct
      421 gcatgatggg gcctgattat acccgccctg atactccgaa ggccgacgcg tggcgtttga
      481 cgcccgcgac agccgagtcc atcgcgaata tgccctggtg ggagttgttg aaagatgcag
      541 agctgcaaaa actgattcgg gcagcgctgc aggaaaactt ggatctgcag atcgcagctg
      601 cgaatatcga agagtttcag gctcaactga tgatcgccaa gttcgatctg gttccctcgc
      661 tgaattacac agggagtgtc ttcggttttc gaaataccaa tgacaacgtc gtcccgcttg
      721 ctggtggcgc tcttccgaca accacgcctg gtaagggagg gggattgagc ctctcgcacg
      781 aagccgccga ggtcggtctc aaatgggagg tggacctttg gggtcgcatc cgccggtcga
      841 tcgaatcggc tcgggcgcaa ttgctatcga aagaggaaaa tcagcgtgcg gtgatcctcg
      901 gattggtcag cacagtggcc caatcatatt tcgatctccg agggctggac tacgaggtgg
      961 aaatcaccaa gcgtaccctt aaagcgtggg atgaatctgt tcgattgtca gagcttcgct
     1021 acaaaaatgg ggttatcccg aagctcgacc tcgatcgctt ccaggcggaa cgggctggga
     1081 ctgcggctca attggcggat ctggagcggc aagtcgtgca gacagagaac cggcttaata
     1141 ttctgctcgg ccgaaggccg gcagagatcg cgagaggact tcacctgacc aaccaatcga
     1201 tgccacctgc cgttccgcca ggactacctt ctgatcttct tcagcggcga ccggacatct
     1261 taaaagtcga gcaggacctc gccgcgacga acgcaaacat cggtgtcgcc caagcaaccc
     1321 ggtttccgca acttgctctg agtggtggga ttggtggagc cggccttcag ttccaaggaa
     1381 gttccttcgg gccgttcgcc accttcaaag ggtctgcctc gttgaccggc ccgctcttta
     1441 acgcatccgc attggggtat caggtgaagg cgaccgaagc tcagactcaa gcagcggtcg
     1501 cccaatacag aaagacgatc atcaccgcat tccaggaagt ggaggatgca ctgattgccg
     1561 tgcagaagac cggcgagcaa cgaatcgcac aggaacaaca agtagctgcc ttggaatccg
     1621 catacaaact ggccgatttt cgctaccagg gaggacgggc cagttatctc gatgtgttga
     1681 cggctcaacg gagtctgttt gatgcggaac ttggtctggc tcgcacgcgc cgcgcccaat
     1741 taatatccgt cgtccaactg tacaaaactc ttgggggagg gtggtcaccg gaaatggctg
     1801 gcaaaaagac acagactgcc gggggtcgcg gagaagcagc tgaatcgacc gaagccatcc
     1861 ccgttgccat tcgctagaga aagaggttcg ctatgtggca ggctatgttc atccagacct
     1921 cgaggagccg aatgcacgcg gcggtggccc tagtggcaac gctcattgcc atgggctgcg
     1981 cctcgaccgt ggtcaagtca gagtatgaga gcgcgaagtt gcgcttgcag cggccgtccc
     2041 aaatttttgt gtacaacttc gcggtcacgc ggcaacaggt gaaggaaaat cagggattgt
     2101 tccagggaac cgtcaatgag ttcaaagggg aaacgacgtt tgagcatgaa ggagaaatcg
     2161 cggaagaggt tcagacggtt gcggcggacg aactcgtgga aaggatccaa aacctcggat
     2221 taccggctaa gaaagtgtcc ggtacgaccc cattacctaa aggttccttg ggcatcaccg
     2281 gacaatttct cgacgtggat gaggggaatc gagccagacg attagtgatc ggattcgggt
     2341 cgggacagtc taaggtcgat attcgaattc aattgtacgg atttgggctg gatccatcca
     2401 gaacggaacc agagatcgct ccaaccaaac tgttggaatt cgaaacccat gccgacagcg
     2461 gctctatgcc aggcgcggca gtcacggccg gcgctggcgc cgcggccggc gctggtgctg
     2521 gcgcggtggt tggggtgaat gccgcgatcg ggggagcgaa atcgtatcgg tccagtatgg
     2581 gagtcatgac ggcgaagagt gcccagcagg ctgtggattt gatgtccgag ttcttcgggc
     2641 ggcagggctg gatcgagcgt gacaaagtga aatatgcgga tcggccataa agtcgggggt
     2701 cgctagccat ggcatggaca ggggctattg ggggagtcag gtggttcgac acagtcgctg
     2761 atttggttcc cacccccgtt ctttttacag agtacacgat gagagtgctt ctttggatcg
     2821 gggtcttcgt ctttgccgtc acactgacgg gctgtgcgag tacctaccaa gccgagccga
     2881 aacaatctgg atttctcggc gactattcgc tgctcaagaa aggtggagag ggggaagcgg
     2941 cccttgtgta tcgtaagtac gacaatgacc gtaccaagtg gcggtcctat tctgagattc
     3001 tcctggaacc ggtgcaaatt tggaagggtg agaagacgga gctcaatcca gacgacgcag
     3061 agtatttggc gaaaattgtc tggtcacgac tcgatgaaga gcttcgcaaa gattacaaaa
     3121 tgacgagtag cccaggtgtc ggggtgctcc gtatccagac tgcggtcacg gaagcgggaa
     3181 ccaacatgcc cgtcctcgat ctcgtcacca ctctctatcc aggaacacgt ctcatttcgg
     3241 aaggaaaacg atgggcgagc ggtaccgaat cgt
//
LOCUS       DATDNW010000315         4756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1203023, whole genome shotgun sequence.
ACCESSION   DATDNW010000315 DATDNW010000000
VERSION     DATDNW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203023"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2752
                     /locus_tag="VI359_07465"
     CDS             <1..2752
                     /locus_tag="VI359_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY6974127.1"
                     /translation="AQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVG
                     VKGEGKVFGSSEEVRIAFDAREVEEHARIKVRIEGALVQTTVGRVLLSEVLPPGMPFV
                     NANKVMTKKEVTRLIDTVYRQSGHRETVTFLDKIKDIGFQYATRAGVSICIDNMHIPT
                     KKEDLIGKAQREVNEIERQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGD
                     PTKVESFNPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVL
                     QYFISTHGARKGLADTALKTANSGYLTRRLVDIAQDVIINEIDCGTTDGIIVSALVEG
                     GEIIQPIEERVLGRLAAEDIRDPVTGEIIVSFNEEIDEDRTKSIVEAGVDRVKIRSVL
                     TCQSRRGVCQSCYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKV
                     VEQTVLEAKHAGHLKFMSFDAKKNADVHNGGIAVRNKEGEWVVMNRNAKIAIVDDNGR
                     EREKYPVVYGGKIKIKDGDRVELGQRLVEWDPYSLTILTEVGGKVAYGDIVEGVTMKE
                     EFDEVTGLSRKVIIEHSGATLRPRVSIKDEGGKTAKVSGSTATVARYLLPVGAHIFVE
                     KGATVHPGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGF
                     VKGQRKVLVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPN
                     ELQKYLVDEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTTFLPGSQVSKMVF
                     EEENDRVLAKGGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDN
                     LRGLKENVIVGRLIPAGTGFEEYRDTFVISPKPEPVAVGQSEPPALVREGAAASSTEG
                     RS"
     gene            2874..3245
                     /gene="rpsL"
                     /locus_tag="VI359_07470"
     CDS             2874..3245
                     /gene="rpsL"
                     /locus_tag="VI359_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY6974128.1"
                     /translation="MPTINQLVRKGRQLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVTDRKQGRSKYGAKRPK"
     gene            3285..3755
                     /gene="rpsG"
                     /locus_tag="VI359_07475"
     CDS             3285..3755
                     /gene="rpsG"
                     /locus_tag="VI359_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY6974129.1"
                     /translation="MPRSRFLHQREPLPDVRYRDKLVGKFLNILMERGKKSTAERICY
                     GAFDMIQEKTGGDPLKVFRTAIDNVKPIVEVKSRRVGGASYQVPVEIRPARRMSLALR
                     WLSEFSRTRGGKSMREKLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            3869..>4756
                     /locus_tag="VI359_07480"
     CDS             3869..>4756
                     /locus_tag="VI359_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY6974130.1"
                     /translation="MARQTSLERTRNIGIMAHIDAGKTTTTERVLYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWKDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYRVPRIAFMNKMDRTGADFYFSVQSIVDRLGANPV
                     PIQLPIGREAEFRGSIDLVTMKGFFYDDETLGAKYKIDEIPPDMIDKAKEYREKMLEA
                     VAEFDDHVMEKYLNGQSLSEEEVRRCIRAGAIAMKITPVLCGSAFKNKGVQQLLDGVV
                     DYLPSPLDIPAVKGIEPNSG"
BASE COUNT         1127 a         1165 c         1524 g          940 t
ORIGIN      
        1 agcacaggtc gaagcccgtg tgctgatgat gtcgattaac aatatcctat ctcccgcgaa
       61 cggaaaaccg atcgcggtgc cgtcccaaga catggtgttg ggatgctact ggttgacgaa
      121 ggaacgggtt ggggtcaagg gcgaaggcaa ggtgttcggc tcctcggaag aagtgcgtat
      181 tgcctttgac gcccgggaag tcgaagagca cgcacgtata aaagttcgca ttgaaggggc
      241 gctggtgcag acgacagtcg gccgcgtgct cctgtccgag gtcctccctc cggggatgcc
      301 gttcgtcaat gcgaacaagg tgatgacgaa gaaggaagtg acacggctga tcgatacggt
      361 atatcgccaa agcggacatc gggaaaccgt gacctttctc gataagatca aggacatcgg
      421 gttccaatat gcgacgcgcg ctggcgtgtc gatttgtatc gacaacatgc atatcccgac
      481 caagaaagaa gatctgatcg ggaaagcgca acgcgaagtg aatgaaatcg aacggcagta
      541 ttccgagggg ctgattacga acggcgagcg ctacaacaag gtcatcgaca tttgggccca
      601 cgtgaccgag caggtagcca atgagatgat gaaagagctc ggggccggag gtgatccgac
      661 gaaagtcgaa tccttcaatc cgatcttcat gatggccgac tccggtgcgc ggggcagttc
      721 gcaacagatt cgtcagctgg gtgggatgcg cggattgatg gccaagccat ccggcgaaat
      781 tattgaaacg ccgattaccg cgaacttccg cgaaggcttg accgtgttgc agtacttcat
      841 ttcgacgcac ggtgcacgga aaggcttagc cgatacggcg ttgaagaccg cgaactcggg
      901 ctacctcacg cgtcgtcttg tcgacatcgc gcaagatgtc atcatcaacg aaatcgactg
      961 cggaacgacg gacggcatta tcgtgagcgc gctcgtcgag ggcggcgaaa ttattcagcc
     1021 gatcgaagaa cgagtgttgg gccggttagc ggccgaggat atccgcgatc cggtgaccgg
     1081 cgaaatcatc gtgtccttca acgaagagat tgacgaggac cggaccaagt cgattgtcga
     1141 agcgggcgtc gaccgggtga aaattcgttc cgtgttgacc tgccaatcgc gccgaggggt
     1201 ctgccagtcc tgctacggac gtgacttggc tcgtgggcgt ctggtcgaaa agggtgagcc
     1261 cgtcggtgtg attgcagcgc agtcgatcgg cgagccgggc acgcagttga cgatgcgaac
     1321 cttccatatc ggtgggacgg ccagtaaggt ggtcgagcaa accgtcctcg aagccaagca
     1381 tgccgggcat ctgaagttca tgagctttga tgcgaagaag aacgccgatg tgcacaacgg
     1441 cggtattgcc gtcaggaaca aggagggcga gtgggttgtc atgaaccgca atgcaaagat
     1501 cgcgatcgtg gacgacaacg gccgtgagcg cgaaaaatat ccggtcgtgt acggcggcaa
     1561 aatcaagatc aaggacggcg atcgcgtcga gctcggtcaa cgactggttg aatgggatcc
     1621 ctactcgctg acgatcctga ccgaggtcgg cgggaaagtt gcctatggag acatcgtgga
     1681 aggtgtcacg atgaaggaag agttcgatga agtgacgggt ctttcccgga aagtcatcat
     1741 cgaacattcg ggtgccacgc ttcgtcctcg cgtatcgatc aaggatgaag ggggaaagac
     1801 ggccaaagtg tccgggagta cggccacagt ggcgcggtat ctgttgcccg tgggcgccca
     1861 catttttgtg gaaaaaggcg ccacggtgca tccgggtgac gtcctggcca agattcctcg
     1921 cgagacaacg aagacaaagg acattaccgg agggttgccg cgggttgccg agttgttcga
     1981 ggcgcggaag ccgaaagaac aggcggtcat cagcgaaatc gatggtgaag tgtcgtacgg
     2041 cggtttcgtt aagggccagc ggaaggtgct cgtcgataac aagatgggag atgtgaagga
     2101 gtatttcatt cctaagggca aacacgtcaa cgtccatgaa ggtgattggg tcagggccgg
     2161 tgagcccttg atggatgggt cggccaatcc gcatgacatc ctggatgtgc tcggacccaa
     2221 tgaattgcaa aaatatctcg tggatgaagt gcaggacgtg tatcggctgc aaggcgtctc
     2281 gatcaacgat aagcacattg agattatcgt tcgacagatg ttgcgaaagg tccgcattga
     2341 agatcccgga gacacgacgt tcttgcccgg tagccaggtc agcaaaatgg tgtttgagga
     2401 agagaacgat cgcgtgttgg ccaagggcgg caagccggcg ctcggcaagc cggtcctgct
     2461 tggcattacg aaggccgcgt tgactaccga tagtttcatc tctgcggcct cgttccaaga
     2521 aacgacgcga gtgcttacgg aagcagccat caacggacgt gaagataatc tgcgaggctt
     2581 gaaagaaaac gtgattgtcg gccgcttgat tccggccggc acagggtttg aggagtatcg
     2641 agataccttt gtgatcagtc ctaaacccga gcctgttgca gttggccaga gcgagccgcc
     2701 cgcgctagta cgggagggcg ctgccgcctc ttcaacagaa ggcaggtcat aacggatatc
     2761 acggaacgag cgtcgcttga cagcgagcaa tgcggtcatt ataatccgca ggctttgccc
     2821 agaaactaga tcgcacaacg gtgtgttgtg ctcaacgcga gagtaattga atcgtgccca
     2881 caatcaatca gttggtcaga aaagggcgcc agctggcgca tgcgaaaaca aagagtccag
     2941 cgctgaagtc ctgtcctcag aagcgcgggg tgtgtctccg ggtctatacg tcgactccca
     3001 agaagccgaa ctcggccttg cgaaaagtcg cgcgcgtccg gttgacaaat ggaatggaag
     3061 tgacgacgta catccctggt gttgggcaca atcttcagga gcactccatt gtgttggtgc
     3121 ggggcggtcg tgtgaaggac ttgccgggcg tgcgctatca catcgtccgt ggtgcgctgg
     3181 atgccgtcgg ggtgacggat cgaaagcagg gccgttcgaa gtatggtgcg aagcgcccca
     3241 agtagggtca gcggcttcga cgaatagtaa aaaattggaa tcgtatgcca cgaagtagat
     3301 ttttacatca gcgggaacct cttccggatg tgcggtatcg cgacaagctt gtcgggaagt
     3361 ttttgaatat cttgatggag cgcgggaaga agagtacggc tgagcgcatc tgctacggag
     3421 ccttcgatat gattcaggag aagaccgggg gcgatccgct caaagtattt cggacggcga
     3481 tcgataacgt gaagccgatc gtggaagtca agtcacgtcg ggtgggtggt gcctcgtatc
     3541 aggtgccggt agaaattcgc cctgctcgtc gcatgtcatt ggcgcttcga tggctgtctg
     3601 agttttcacg gacgcgtggc ggcaagagca tgcgagagaa acttgccgca gagctgctcg
     3661 acgcgtcgaa caacacaggg gcgtcggtga agaagcggga agacgtgcat cggatggcgg
     3721 aggccaataa ggcgttcgcg cactatcgct ggtaacgtcg ttctgtgctg cagttgggcc
     3781 gtgctgcgat ccgcagacgc ggggtgcccc tgaatcgcag cgatcgtccg gctcattctg
     3841 cagcacgtgt gtttttaagg gatgaattgt ggctaggcag acatcactcg agcgtacgcg
     3901 gaacatcggc atcatggcgc acatcgatgc cggcaagacc acgaccacgg agcgtgtcct
     3961 ctactacacg gggatgacgc acaagatggg tgaggtccat gagggcgcgg cgacgatgga
     4021 ctggatggag caggaacggg agcgcgggat tacaatcacc gccgctgcca ccacctgttt
     4081 ttggaaggac catcgcatca acattattga tacgcccgga cacgtcgatt ttacaatcga
     4141 ggtcgagcga tcgctccgcg tgctggatgg cgccgtcgct gtgtttgatt ccgtgcaggg
     4201 agtagagccg cagtcggaga ccgtgtggcg gcaagcggac aagtatcgag tgccgcgcat
     4261 tgcgttcatg aacaaaatgg accgaaccgg cgcagatttc tacttcagcg tgcagtctat
     4321 cgtggatcga ctgggagcaa acccggtgcc aatccagctg ccgatcgggc gtgaggcgga
     4381 gttccgcgga tcgatcgatc tcgttacgat gaagggattt ttctacgacg acgaaacact
     4441 gggggccaaa tacaagatcg acgagattcc tccggatatg atcgataagg caaaagaata
     4501 tcgggagaag atgctcgaag cggtcgcgga gttcgacgac cacgtcatgg aaaaatacct
     4561 caatggtcaa tctttgagtg aggaagaagt gcggcgatgt attcgagccg gggcaattgc
     4621 catgaagatc acgccggttc tctgcggctc ggccttcaaa aacaaaggcg tccagcagct
     4681 cctcgacggc gttgtcgatt acctgccgtc gccgctcgat attccggccg tgaaaggaat
     4741 tgagccgaat tctggg
//
LOCUS       DATDNW010000316         4571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1203534, whole genome shotgun sequence.
ACCESSION   DATDNW010000316 DATDNW010000000
VERSION     DATDNW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203534"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /gene="purH"
                     /locus_tag="VI359_07485"
     CDS             <1..1639
                     /gene="purH"
                     /locus_tag="VI359_07485"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY6974131.1"
                     /translation="KSIVSFAILRALLAALICFIHASMKGCHMAAIQRALISVSDKTG
                     VVDMAKGLEALGAEILSTGGTAKTLREAGIKVTEVAAYTGFPEILDGRVKTLHPKIHG
                     GLLGRRSLPTHVDQMKQHAIGPIDVVVVNLYPFEATIAKPHSTFDEAIENIDIGGPSM
                     LRSAAKNHEDVLVVVDPADYARVLNAIKAGITTRALRRELALKVFQHTARYDGVIAGY
                     LEKQVQGSEVKFPKILSLQYELVEPLRYGENPHQQGAFYRELHSDEPSVSRGKILHGK
                     AMSYNNFLDANSALELAKEFDETAVAIIKHNNPCGAALGTTPVEAYVKARETDPVSAF
                     GGVIAFNRPVDVTAAKEITSTFVEVVIAPGFTEDALAELKRKKDLRLLDVGPLTNVKR
                     EGYDLKKLVGGLIVQDRDLGVLTDLRTLHVPTVRKPTDEEYAACAFAWKVCKHVKSNA
                     IIYAKPGQTVGIGAGQMSRVDSVKLAAMKAHMPVKGCVMASDAFFPFRDGLDAAAQAG
                     ITAVIQPGGSIRDAEIIKAADEHGLAMIMTGMRHFRH"
     gene            1701..2984
                     /gene="purD"
                     /locus_tag="VI359_07490"
     CDS             1701..2984
                     /gene="purD"
                     /locus_tag="VI359_07490"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY6974132.1"
                     /translation="MKILVVGSGGREHAIVWKLAQSPRAPQLYCAPGNAGIEALATCV
                     SIPADDITGLKQFVQQERIDLTVVGPEMPLALGIADEFRKAKLKIFGPTRNAARIEAS
                     KSFSKDVMVTAKIPTAQARSFDTMADALAYLDQHDLPVVIKADGLAQGKGVIIATTRE
                     QAKQAVVDSMERAVFGQAGHRVLIEQYLDGEEVTIMAFTDGDTVVPMLPAQDHKRVGD
                     GDTGLNTGGMGAYCPAPLGTRALCETVTREVLRPIVDTLARLGCPFQGVLYAGLMIVK
                     GAPYVLEFNARMGDPETQVVLPLLKTDLVDVIEAVVDHRLDQLAVEWQDESAVCVVMT
                     SNGYPGPYQHGFVIHGLPTAEKATAVVFHAGTARRGTDIVTAGGRVLGITGRGKTIAA
                     AQQEAYRVVKNVTFNGCHFRTDIAHRALHPKNDRH"
     gene            3002..3658
                     /locus_tag="VI359_07495"
     CDS             3002..3658
                     /locus_tag="VI359_07495"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY6974133.1"
                     /translation="MPVIKRFHPNELATLCVHVSAVLRQGGIVAIPTETYYGLGVDPF
                     NPAALRRLATVKGRAEGSPILLLIGAREQLSDLVDTIPPTAAALMEAFWPGPLTIVFR
                     ARATIPTLVTAGTGTVGVRWTSLAALADILRVVGPLTGTSANRSGERAARTAQEVQQA
                     LGDEIDLIIDAGAMPGGLPSTVIQVQGQTQIVREGTITRACIRETLRARGFFLNEGQM
                     "
     gene            3711..4124
                     /locus_tag="VI359_07500"
     CDS             3711..4124
                     /locus_tag="VI359_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974134.1"
                     /translation="MGDGRERGGWIGFCIVVGMGLMLAGCDYWPPALQTQIEQLRSEV
                     QAATAERAQLQNQLTALTKVKDDLQAQVDDLSRANREKTSMINSLQSSLAAAQERLAK
                     QAKPAPAKTSTAKAPAKSSSKVAAKAPAKKKPTTH"
     gene            complement(4146..4517)
                     /locus_tag="VI359_07505"
     CDS             complement(4146..4517)
                     /locus_tag="VI359_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY6974135.1"
                     /translation="MPIYEYVCDGCAYRFERKQSIKDDPVTTCDRCGKSVTRLISSPA
                     IMFKGSGWYVTDYSDKLKPPAGSEPAAPGVAEKGAADKKDSSSSSSSSPAPSPSSSSD
                     SPSSSPSTSSGTSTPTSTTTK"
BASE COUNT          985 a         1220 c         1441 g          925 t
ORIGIN      
        1 gaagtccatc gtctcctttg ctattctgcg cgcgcttctc gcagcactca tttgtttcat
       61 tcacgcatcg atgaaggggt gtcacatggc cgccattcag cgggcgctga tcagtgtgtc
      121 cgacaagacg ggagtcgtgg acatggccaa agggttggag gcccttggtg ccgagattct
      181 gtccacgggt ggaacggcaa aaacacttcg tgaggcgggg attaaggtga cggaggtcgc
      241 cgcctatacg ggatttcctg agatactgga tggccgggtt aagacgctcc atcccaagat
      301 tcatggaggt ttgctcggcc gtcgcagtct gcctacgcat gtcgatcaga tgaagcagca
      361 tgccatcggc ccgatcgacg tcgtggtcgt gaatctctat cctttcgaag cgacgatcgc
      421 aaagccccat agtacctttg atgaggcgat tgagaacatt gacatcggtg gaccgtcaat
      481 gctccgatcc gccgccaaga atcacgagga tgtgctggtt gtcgtcgatc cagcggatta
      541 tgcgcgggtg cttaacgcca tcaaagccgg gataacgaca cgcgcgcttc gccgggaact
      601 cgcgttgaaa gtgttccaac atacggctcg ttatgacgga gtgattgccg ggtatctcga
      661 aaagcaggtg caggggagcg aagtgaaatt tcccaagatc ctgtcgctgc agtacgagct
      721 ggtcgagccg cttcggtatg gcgagaaccc gcaccagcaa ggagcgttct atcgagaact
      781 gcactcggat gaaccgtctg tctcgcgcgg aaagattctg catggcaagg cgatgtctta
      841 taataatttc ctcgatgcga actccgcgct cgaactcgcg aaggagttcg acgagacggc
      901 ggtggccatc atcaaacaca acaatccctg cggcgccgcg ctgggcacga cgccggtcga
      961 ggcgtatgtc aaagcgcgtg agaccgatcc ggtgtccgcc ttcggcgggg tcattgcatt
     1021 caatcgaccc gtcgatgtga ccgctgccaa ggaaatcacc tcgacattcg tcgaagtcgt
     1081 aatcgcgccg ggatttacag aggatgcact ggcggagctc aagcgcaaaa aagatctgcg
     1141 tttactcgac gtcggaccgc tgacgaacgt gaagcgggaa gggtacgatc tcaagaaact
     1201 tgtaggcggg ttgatcgtgc aggatcggga ccttggcgtc ttgaccgatc tccgtacgct
     1261 gcacgtgccg accgttcgca aaccgaccga cgaagaatac gccgcctgtg cctttgcctg
     1321 gaaggtgtgt aagcacgtga agtcgaatgc catcatctat gcaaagcccg ggcagacggt
     1381 ggggatcggt gccggccaaa tgagccgcgt tgattctgtg aagctggcgg caatgaaagc
     1441 gcacatgccg gtgaaggggt gtgtgatggc gtcggatgcc ttctttccgt tccgcgatgg
     1501 actggatgcc gctgcgcaag ccgggattac agcggtcatt caaccgggtg ggtcgatccg
     1561 cgacgcggaa atcatcaagg cggctgatga acatggcctg gccatgatca tgacaggcat
     1621 gagacacttt agacattgat tgacacacca gattcacggc cgactgtcca gtattcccgc
     1681 ctcactgata aggttcacat gtgaagattc ttgttgtcgg cagcggcgga cgcgagcatg
     1741 ccatcgtgtg gaaattggcc cagagcccac gagcacccca actatattgt gcgccgggca
     1801 atgccgggat tgaggccctg gcgacctgcg tctccattcc agccgacgat atcaccggac
     1861 tcaagcagtt tgttcagcaa gagcggatcg acctgaccgt agtcgggccc gagatgccgt
     1921 tggcgttggg gattgccgac gaatttcgga aggccaagtt gaagatcttc ggtccgacgc
     1981 gcaacgcggc tcgaatcgaa gcgagtaaga gtttctcaaa agacgtcatg gtgaccgcca
     2041 agattccgac ggctcaggcg aggagcttcg acacgatggc ggacgcgttg gcatacctgg
     2101 atcagcatga cctcccagtc gtgatcaagg ctgacggact tgcgcagggc aaaggggtga
     2161 tcattgcgac gacgcgcgag caggctaaac aggcggtcgt ggattcgatg gaacgtgccg
     2221 tgttcggtca ggccgggcat cgcgtgttga ttgagcaata tctcgatggc gaagaagtca
     2281 cgatcatggc cttcaccgat ggcgacactg tcgtaccgat gttgccggcg caggatcaca
     2341 agcgtgtcgg tgacggggat acggggttga acaccggcgg gatgggagcg tactgcccag
     2401 ctccgctcgg cactcgcgcg ctctgtgaga cggtcactcg cgaggtgctg cggcccatcg
     2461 tcgatacgct cgcgcggctc ggctgtccat ttcaaggcgt gttatatgcc gggttgatga
     2521 ttgtgaaggg cgctccctac gtgttggaat ttaacgcacg gatgggcgac cccgaaacgc
     2581 aagtggtgtt gccattgctg aagacggatc tcgttgacgt gatcgaagcg gtcgtggatc
     2641 atcggctcga ccaactcgcg gtggaatggc aggacgagtc agccgtgtgt gtggtcatga
     2701 cgtcgaatgg gtaccccgga ccgtatcaac acgggtttgt gattcacggc ctgccgactg
     2761 cagagaaggc aacggctgtg gtgtttcacg ccgggaccgc gcgccggggg actgacatcg
     2821 tcacagccgg cggacgtgtg ctgggaataa ccgggcgcgg caagaccatc gccgcggcgc
     2881 agcaggaagc ctatcgtgtc gtgaagaacg ttacgtttaa cggctgccat tttcgaaccg
     2941 acatcgcgca tcgtgccctc cacccgaaaa acgatcggca ttgattgacc ggcacctctc
     3001 catgcctgtg atcaagcggt ttcatccaaa cgaattggcg actctgtgcg tccacgtgag
     3061 cgccgtcctt cgacaggggg ggatagtcgc gatccctacg gaaacttatt acggactagg
     3121 tgtggatccc ttcaatccgg cggctctgag gcgtctcgcg acagtcaaag gccgtgccga
     3181 aggcagcccg atccttctcc tcataggtgc acgggagcaa ctttctgatc tcgttgacac
     3241 tattcctccg acagcggcag cgttgatgga agcattctgg cccggccctt tgacgatcgt
     3301 atttcgggcg cgggctacca ttcctacact tgtcacagcg ggaaccggaa cggtgggagt
     3361 ccggtggacg tccttggctg ctctcgcaga tattcttcgg gttgttggtc cactgacggg
     3421 cactagtgcc aatcgatcgg gggagcgcgc ggctcgaacg gctcaagagg tacagcaggc
     3481 gttgggtgac gagatcgatt tgatcatcga tgctggcgca atgcccggcg ggttgccatc
     3541 aaccgtaatt caggtccagg gacagacaca gattgtgcgt gaaggaacga tcacgcgagc
     3601 ctgcatccga gagacgttgc gcgcgcgagg atttttcctc aatgaggggc agatgtgata
     3661 tacaacagtg gcagtgtgca tttacgcata gggcagaaag gaggaaaact atgggagatg
     3721 gtcgagagcg cggcggatgg atagggttct gtattgtggt aggaatgggg ttgatgctgg
     3781 cggggtgtga ttactggccg ccggcgttgc aaactcagat cgaacaacta cggtccgagg
     3841 tgcaagcggc gacagccgaa cgggcacaat tgcaaaatca actgacggcc ctcacgaagg
     3901 tcaaggacga tctgcaggca caggtcgatg acttgagtcg agcgaatcgc gagaaaactt
     3961 ccatgatcaa cagcctgcaa agcagcctcg cggcggcgca ggagcgcttg gccaagcaag
     4021 ccaagccggc accggcgaaa acatcgaccg ccaaagcgcc ggccaaatca tcgtcaaagg
     4081 ttgccgccaa ggctccggcg aagaagaaac cgacgacaca ctagcgcgac ggagccggtc
     4141 agctcctatt tcgtggtggt tgaggtcggt gtcgaagttc ctgaggacgt cgagggggaa
     4201 ctcgaaggac tgtccgaaga tgaggaaggt gagggggccg gcgacgacga ggaacttgat
     4261 gaactgtctt ttttgtcagc ggctcccttt tcggctaccc ccggggccgc aggctcactg
     4321 ccggcgggcg gcttgagctt atctgagtag tctgtcacat accagccgct gcctttgaac
     4381 atgatagcgg gggaggagat caatcgcgta acggatttcc cgcaccggtc gcaggtcgtc
     4441 accgggtcgt ctttgatgct ttgtttccgt tcgaaccgat acgcacagcc gtcgcacaca
     4501 tattcataga ttggcacgga acgatcctcc tcgcgagtat ggtcttgttg ctctcatgct
     4561 ggcacgacga c
//
LOCUS       DATDNW010000317         8343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_318157, whole genome shotgun sequence.
ACCESSION   DATDNW010000317 DATDNW010000000
VERSION     DATDNW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318157"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VI359_07510"
     CDS             complement(<1..314)
                     /locus_tag="VI359_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY6974136.1"
                     /translation="MLSASLKPRLSSRPTPSARERSLVPIGMATILPHAARQVRRLER
                     DFLGEVGRWGYEEIILPTLEYLDVLAPGLEAELVEKSYKLADRTTGRMLLLRPDATAQ
                     IAR"
     gene            complement(341..1924)
                     /gene="serA"
                     /locus_tag="VI359_07515"
     CDS             complement(341..1924)
                     /gene="serA"
                     /locus_tag="VI359_07515"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY6974137.1"
                     /translation="MKILVSDSLSKQGVELLEKAGFTVVVKTKLSKEELFKEIKDADG
                     LIVRSGTKVTAELIEAAECLKVIGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMSMICAMSRRIPQATASIRAGKWEKEKFMGVELYNKVLGIVGVGQIGGHLTKLAQG
                     MGMNVIAYDPYLSEERAQKMGITILELDELFRRADIISVHTPLTPETRGIINAQAIAK
                     MKPGVMIVNCARGGIVHEGDLLEALKTKRVAAAAFDVFDEEPVKPDHPLLSLENFICT
                     PHIGASTAEAQENVAVGIAEQIVDYFTKGIARGAVNIPSVPPDVLPRLQPYLALCEQL
                     GLLQTQLCEGGLERVTVEYSGEVVNLTVAPMTIAVLKGLLTPIMEAPVNYVNAPIVAK
                     ERGIEVKEVKSSDAGDYISLIRVRVEAGKRSHQVAGTLYHKKDPRIVEIDQFKVEVVP
                     EGHMLLIQNIDRPGVIGMVGKVLGDNNINIVRMQCALEKRGGNALLIIGSDMAFPQSV
                     LEKIKSSDNILSVKVAGLS"
     gene            complement(1968..3110)
                     /locus_tag="VI359_07520"
     CDS             complement(1968..3110)
                     /locus_tag="VI359_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY6974138.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRDG
                     LKWLFQTRNDVLMLAASGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTEIKVEWGHVVNPQMIADALKKDPGIKAVYVQASETSTGVAHDVKTLAQIVKAHGDT
                     ILVVDAITALGVFDIKTDDWGIDVLITGSQKALMLPPGLAFVSVSDKAWQLADKAKNA
                     AFYFNFKKERENQQKNQTAFTPAVSLIIGLQEVLKMLKAEGLDAVFSRQAGMAHAMRE
                     GVKAAGLALFPKESPSDALTAVSAPEGVDGQAVYKNLRVQYGITAAGGQDHLKGKIFR
                     VSHMGYMDRFDVITAVAAIEMVLKGLGHPVKLGSGVAKAQELLMAK"
     gene            3818..4774
                     /locus_tag="VI359_07525"
     CDS             3818..4774
                     /locus_tag="VI359_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY6974139.1"
                     /translation="MVDSSVQGHIRYFNTAIRGRFEQWLLRLSRYRPLVETIFTEFHL
                     PSDLVYLSLVESGFNPYAYSRARATGPWQFMKGTAKLYGLRVDQYVDERRDPIKSTVA
                     AARYLRDLYDLFGAWPLAMAAYNAGEGKVMRALQKAQAESFSEISKTRLIRRETKEYV
                     PRFMAATIIARNPDRYGFSQEAVTPHRFEEVIVDRPIHFRAIANVTGVPYEELRLLNP
                     ELRREATPPDDPAYHLKVPVGSKAKVEQLLDRIPTFKFPALRTSRAKFAEVGTSRWYK
                     VRVGDTLEKVSKRFRIPLKTLKAKNNLSGPVIRPGDFLIISH"
     gene            complement(4831..5613)
                     /locus_tag="VI359_07530"
     CDS             complement(4831..5613)
                     /locus_tag="VI359_07530"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6974140.1"
                     /translation="MTYRIKVPAKTLPVDEAHLLSGVEHALLRLQDYRRPLLVGLGVF
                     LIAIVVVGGVLWFDRQAVQKAQELEREAMLHILAAANNPQKAEASLKQAIATYRQVAD
                     QYPRTPTAPLALFQAGNALVQANDLGGAIEAYQRFIALYGSNPGFAGLVQQRLAYAYL
                     LKGDREQAVKALTSILEMPSALNRDQALFELARIEESQSRPEGATAHYQELIKSYPNS
                     PFASEAMIRTKILDVKKTPDPATASSPVSQTPSGLPPATDKK"
     gene            5768..6928
                     /gene="bioF"
                     /locus_tag="VI359_07535"
     CDS             5768..6928
                     /gene="bioF"
                     /locus_tag="VI359_07535"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY6974141.1"
                     /translation="MFEQHLHKLTTRHLSRRLRTLHSATGPVVELEGRHVLLMASNDY
                     LGLATHPDVVQAAVQATQQYGAGAGAARLISGTFPPHQELELALAVFKKTTAALTFGS
                     GYLANLGTIPALIGRGGLILADRLCHASLLDGSKLSGADLRVYRHSDVGHLQTLLARR
                     GRGRRTLIITDGLFSMDGDLAPLPDIVELAQRYEADLYVDDAHGTGVMGATGRGTIEH
                     FGLESDISFHMGTLGKALGSSGAYVVGPSTLIEYLVNTCRSFIFTTAPPPASAAAAAA
                     ALRVLQREPDRRTRLWANRERLASGLKHLGFRLAASVSPIVPILVGDAEKAVTFAEQL
                     FAEGVYAPAIRPPTVPESTSRIRVTITSEHTLLHIEQALGAFERAGKTARLL"
     gene            7076..8134
                     /locus_tag="VI359_07540"
     CDS             7076..8134
                     /locus_tag="VI359_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY6974142.1"
                     /translation="MDISKLLTFAAKEGASDCHISAGEPPMIRLHGDLKKLDHPPLTS
                     EETHALVYDMMSDAQRKNFEEKRECDFSFELGDIARFRVNVFVQNRGLGAVFRNIPTE
                     IIPMEKLGLPPIVRQLCDREKGLILVTGPTGSGKSTTLASMIDYLNNTFEGHIITIED
                     PIEFVHKSKKCLVNQRELGVHTLSFANALRSALREDPDIILVGEMRDLETIQLALTAA
                     ETGHLVFATLHTSSAPKTIDRIIDAFPPAQQAQIRTQLSEALESVITQTLLKKKAGGR
                     VAALEIMVATTAIRNLIREAKLHQIPGIMQASQKDGMQTMDMALIDLATRGIVNKAEA
                     QSRSMNPNLFGATMGGAA"
     gene            8164..>8343
                     /locus_tag="VI359_07545"
     CDS             8164..>8343
                     /locus_tag="VI359_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEY6974143.1"
                     /translation="MDIRTLLKVMVDQEASDMYLTVDAPPIYRVHGSTEQTDAPPFTN
                     EQLEALALALMRGQQR"
BASE COUNT         1896 a         2432 c         2139 g         1876 t
ORIGIN      
        1 cgggcgattt gagcagttgc atcgggcctg agcaacagca tccgtcccgt cgtgcggtcg
       61 gcgagcttgt aacttttttc aacgagctct gcctcaagac caggagccaa aacatcgaga
      121 tattcaagcg tcggcagaat aatttcttcg tatccccagc ggccgacttc ccctaagaaa
      181 tctcgttcaa gtcgacgaac ctgccttgca gcatgcggaa ggatggtcgc catcccgatc
      241 gggacaagcg accgttcacg cgccgacgga gttggccgcg acgataaccg aggcttcaga
      301 gaggcggaga gcatagctga tcgaggatgc ggcgcggaac ctaggagagg cccgcgacct
      361 ttaccgagag aatattgtcg ctagatttga tcttctcaag caccgactgc gggaaggcca
      421 tgtcagaccc aatgatgagc aaggcattcc ctccccgttt ttccaaggca cactgcatgc
      481 ggacaatgtt gatattgttg tcgccgagta ctttccccac catcccgatc acacctgggc
      541 gatcgatatt ctggatcaag agcatatgac cttcaggcac gacttccact ttgaattgat
      601 cgatctcgac gatgcgggga tctttcttgt gatacagggt cccggcgact tgatgcgatc
      661 gcttcccggc ttctacgcga acacgaatca ggctgatata gtcaccggca tcggaactct
      721 tgacctcttt aacctcaatc ccccgctctt tcgcaacgat tggcgcattc acatagttca
      781 ctggcgcttc cataataggt gtcaagagtc ctttcagcac cgcgatggtc atcggcgcca
      841 ccgtgaggtt cacaacttcc ccactatact caaccgtcac ccgctcgagt ccaccttcac
      901 agagttgcgt ctgcaagaga ccaagttgct cgcagagagc gagatatggc tgcagtctcg
      961 gtagcacgtc cgggggaacc gacggaatat tgaccgcccc tcgcgcaata cccttcgtga
     1021 aataatcgac gatttgctca gcgatgccga cggccacgtt ctcttgtgct tcggccgttg
     1081 aggccccgat gtgaggcgta cagataaaat tctcaagcga gaggagcggg tgatccggct
     1141 tgaccggctc ttcatcaaac acatcgaacg ccgctgcggc cacacgcttg gttttcaagg
     1201 cttcaagcaa atcgccttcg tgcacgatcc cgcctctggc acaattgacg atcatcactc
     1261 ctggtttcat cttcgcaatc gcttgcgcat tgataatccc acgtgtctca ggagtcaacg
     1321 gtgtatggac ggaaatgatg tcagcgcgtc ggaagagttc gtccagctcc aagatcgtga
     1381 tacccatttt ttgagcccgc tcttcggaca agtacggatc ataggcgatg acgttcatcc
     1441 ccatgccttg ggccaacttc gtgaggtgtc ctccgatctg cccgacgccg acaatgccga
     1501 gtactttgtt atagagttcc acccccatga acttttcttt ttcccactta ccggctctta
     1561 tcgatgccgt ggcttgaggg atacgacggc tcattgcaca gatcatcgac atcgtatgtt
     1621 cagccgtcgt caccgtgttg ccgcccggtg tgttcatgac cacgatgccg cgtcgcgtgg
     1681 cggccggcgt atcgacgtta tcgagccccg aaccggccct gccgattact ttcaaacatt
     1741 cggccgcctc gatcaattcc gcagtgacct tcgtccccga tcggacaatc aacccatcag
     1801 catccttgat ttccttgaat agttcttctt tagacagctt cgttttcacc acgacggtga
     1861 atcccgcttt ctccagtaac tccacccctt gtttcgaaag actatcgctg acgaggattt
     1921 tcataagatt cttgctcgac ctttgaacgc gatttcgtgc ggggctctca cttcgccatg
     1981 agaagctctt gcgcctttgc cacgccgcta ccgagtttta caggatgccc caggcctttc
     2041 agcaccatct cgatcgctgc cacggccgta atcacatcga aacgatccat ataccccata
     2101 tgggacacac ggaaaatctt tccttttaag tgatcctgcc cacctgcggc tgtgatgcca
     2161 tactgaacgc gcaaattttt atagaccgcc tggccatcga ctccttcagg agcggaaaca
     2221 gctgtcaagg catcgctggg tgattctttt gggaagagcg cgagaccagc cgccttgacg
     2281 ccttctcgca tcgcgtgagc catgcccgct tgacgactaa acacggcgtc gagtccctct
     2341 gccttgagca tcttgagaac ctcctgcaaa ccgatgatca aggaaaccgc aggagtgaag
     2401 gccgtctgat tcttctgctg gttttctcgt tctttcttaa agttgaagta aaacgcagcg
     2461 ttcttggcct tatccgctag ctgccaggcc ttgtcactca cgctgacgaa cgccagcccc
     2521 ggcggcaaca tcaaagcttt ttgggatcca gtgataagaa cgtcgatccc ccagtcatcc
     2581 gtcttgatat caaaaactcc caaggccgtg atcgcatcga ccacaaggat agtgtcccca
     2641 tgagccttga caatctgtgc cagggttttg acgtcgtgag ccacccctgt cgatgtttcg
     2701 ctggcctgca catagacagc cttgattcca gggtcctttt tcaatgcatc agctatcatt
     2761 tggggattca cgacatgtcc ccactcaacc ttaatttccg tcacctgagc tccgaacgtc
     2821 ttacacagct tggtccagcg ctctccgaac ttgccgccat taattgtgag agccttgtcg
     2881 ccgggagaaa gaaaattcga taccgaacct tccataccgc cggtgcctga ggctgctaac
     2941 atcaagacat cgtttctcgt ctgaaacagc cacttcaaac cgtcccgaac ttccgcaaaa
     3001 agcttgtcaa attccggagc tcggtgatga atcatgggcc tcgccatcgc caaaagcacc
     3061 tctggcggga cgggcgtggg acccggagcc agcaaatacc gcttcaacat cgacgactcc
     3121 tcctccgatt gggtcgtagc agggaaaact gcgccaggaa ggcgcgtacg ataccactgc
     3181 cctttgaaga gtgtcaaggc aaggaacacc gtaacgcggc atgattgcta tgaacttgtt
     3241 gctaactcgc ctgcacttta atcgagcatg atacgcaacg gaaacgccta cgagcatagc
     3301 ggcatgacac caatgtgggg ggcagtagtg cctgcaattt gttgacaacg tcggtccctc
     3361 tcggtactgt aggttcacat atatggagtc agtacacgac cactcacgaa cgggacttgc
     3421 tggggcgact tggccattgg ccctaatcct tggggtttca attggctctt tgactcagtt
     3481 tgctttggca gccccttcta ttcaaccagg cgaggaatct ccacccattg caggcgaggt
     3541 ggacacttcc gtcagccccg aagacgaacc agacgcgaac ttggtcccag tcgatacgga
     3601 gccccctgcg tccgactcag caggtggtcc gtcgacctcg actgaaatca ccagccagag
     3661 cacagtccca aggtctgaag aactcttgca actcaatcct gccacgagtg gaacagccgc
     3721 tcggttttca gacgtcttga atcctccagc ggaggtcgcc cagtccctgc cctcctcacc
     3781 agagtcggtg gaaccgacgg cttacaatgt tcctatcgtg gtcgactctt cggtacaggg
     3841 gcatatccgt tatttcaata ccgcgattcg agggcggttc gaacaatggc tgcttcggct
     3901 cagtcgatat cgaccgctag tggaaacgat ctttacggaa tttcacctcc ccagcgatct
     3961 cgtgtacctc tcgctggtgg aaagcggatt caacccttat gcgtactcgc gagcccgcgc
     4021 aaccggaccg tggcagttca tgaaaggcac ggcgaaactc tacgggttac gcgtagatca
     4081 gtatgttgat gaacggcgtg atccgattaa atccacggtg gctgctgcac ggtatcttcg
     4141 agacctctac gatctctttg gcgcctggcc gctcgcaatg gcggcctata acgcaggaga
     4201 agggaaagta atgcgagctc tacagaaggc tcaagccgag agtttttctg agatttccaa
     4261 gactcggctc atcagacggg aaacgaagga atacgtcccg cgcttcatgg ccgcgacgat
     4321 tatcgcaagg aacccagatc gctacggatt tagccaggag gccgtcacgc ctcatcggtt
     4381 tgaagaagtc atcgttgatc gcccaattca tttccgagcc atcgcaaatg tgacgggtgt
     4441 gccatatgaa gaacttcgcc tcttgaatcc ggagttacga cgagaggcga cgccacctga
     4501 cgaccccgcc tatcatctca aggtaccggt gggatcgaaa gccaaggttg aacaacttct
     4561 cgatcgcatc cccaccttca agtttcccgc tttgcgcaca agcagagcaa agttcgccga
     4621 ggtcgggaca tcacgttggt ataaagtacg tgtcggagac acacttgaga aagtgtcgaa
     4681 gcgattccgc atccccctca agacgctcaa agccaagaat aacttgtcgg gccccgtcat
     4741 cagacccggc gacttcctca tcatcagcca ctgagtgcac tcacgtcgct agaatgagag
     4801 tatgatcctc gcgcctgcga gaggcgacgg ttatttctta tcagttgcgg gaggcaaacc
     4861 agaaggagtc tgtgatactg gactggacgc tgtggcagga tcgggagttt tcttcacatc
     4921 caggattttg gtacgaatca tcgcctcgct ggcaaacgga gaatttgggt aggacttgat
     4981 caactcctga taatgagctg ttgctccttc gggacgagat tgagattctt ctatccgggc
     5041 taattcaaac aaggcttgat cccggttaag tgccgacggc atctcaagaa tgctggtcaa
     5101 ggccttgact gcctgttccc gatccccctt caggagatat gcataggcca gccgctgctg
     5161 caccagtccg gcaaaacccg ggttcgaacc atacaacgcg ataaatcgct ggtaggcctc
     5221 gattgcgccg cctaaatcat tggcttggac taaagcattg cccgcttgaa acagcgccaa
     5281 cggagctgtg ggcgtgcggg gatactgatc tgcaacttga cgatacgtgg caatggcttg
     5341 cttgagtgac gcctctgcct tctgaggatt attggcagcg gccagaatgt gaagcatcgc
     5401 ttctcgctcc agttcttgag ccttctgcac agcctgtcga tcgaaccaga gcaccccccc
     5461 caccaccaca atcgcaatga gaaatacccc aagccccacg agcaacggac gccgatagtc
     5521 ctgaagtcgc aggagcgcgt gttcaactcc gctcagtaga tgcgcttcat caacaggaag
     5581 cgttttggct ggaactttga tccgataggt cattgcctac gacaccactg ccactgacag
     5641 caaccctgtt ccctctggtt cgccttgcgc cttatactaa ggatgtcgca aagttgtcaa
     5701 tgttgcgttg gcgagccttt aggaagcaac tgtacgatga ttccgctgct acagtagaaa
     5761 cctctggatg ttcgaacaac acctgcataa gttgaccaca cgacatctga gcagacgttt
     5821 gcggacgctc cactctgcca ccggccctgt agttgagctc gaaggacgac atgttctgct
     5881 catggcctcg aacgactatc tcgggcttgc cacccacccg gatgtcgtcc aagccgccgt
     5941 tcaagcaacg caacaatacg gggctggagc cggcgcggca cgattgattt ccggaacgtt
     6001 ccctccccac caagaacttg aactggccct agcggtgttc aagaaaacca ccgcggcact
     6061 gacatttggc tctgggtatc tcgccaatct tggcacgatt cccgctctga ttggtcgcgg
     6121 cggtctgatt cttgccgatc gtctgtgtca cgctagtctt ctcgatggta gcaaattgag
     6181 cggagccgac ttgcgagtat atcgacacag cgacgtggga catctgcaaa cccttctggc
     6241 ccggagagga cgtggacggc gcacgctgat catcacggat ggtctgttca gcatggatgg
     6301 agacttggcc ccgcttcccg acatcgtcga attggcgcaa cggtatgaag ccgatctcta
     6361 tgttgacgac gcgcatggaa ctggtgtcat gggtgcaacc ggccgaggca ccatcgaaca
     6421 tttcggtctt gaatcagaca tctctttcca catgggcacg ttgggcaaag ccctgggcag
     6481 cagcggagcg tacgtggtgg gaccatcgac cctcattgag tatctcgtga atacctgtcg
     6541 ctccttcatc tttacgacag cacctcctcc ggcatccgcg gccgcggccg ctgcggctct
     6601 gagggtcttg caacgggaac cggatcgacg gactcgttta tgggccaacc gggaacggct
     6661 cgcatcgggg ttaaagcacc ttggatttag attggcggcg agcgtcagtc ctatcgtgcc
     6721 aatcctcgtg ggcgatgcag agaaagctgt gaccttcgct gaacagctct tcgcagaagg
     6781 agtgtatgcc ccggcgattc gcccaccgac tgtcccggag tcgaccagcc gaatccgcgt
     6841 gaccatcaca tcggaacaca ctctgctcca catagaacag gcccttggcg cgtttgagcg
     6901 ggcggggaaa accgctcgcc tgctctgact catcgcataa taccgcgtac ctcccgaaac
     6961 gagcgtcagc ttccggcgat tttcttgctt tcccactttg atgtggcatc atgctcacag
     7021 agcactcgac gatccagcga ccgtcatcat tccatctcag cgaggagtca tcttcatgga
     7081 tatttcgaaa ctgctgacct ttgcagcgaa agaaggcgcg tctgattgcc atattagcgc
     7141 cggcgagcct cctatgattc gccttcatgg agacctcaag aaactcgatc acccgccgct
     7201 gacctccgag gaaacacacg cgcttgtgta cgatatgatg agcgatgcgc agcggaaaaa
     7261 ctttgaagaa aaacgtgagt gtgatttttc gttcgagttg ggagatatcg ctcgctttcg
     7321 cgtgaacgtg tttgtgcaga accgtggctt gggggccgtc ttccggaata ttcctactga
     7381 gatcatccct atggaaaaac tcggcctgcc accgatcgtg cgccagctgt gtgaccggga
     7441 aaaaggattg atcctggtaa cgggaccgac cgggtcaggt aagtccacca cgctggcctc
     7501 gatgatcgat tatttgaaca acaccttcga aggccatatc atcacgatcg aggatccgat
     7561 tgagtttgta cacaagtcga aaaagtgtct ggtcaaccag cgtgaactgg gcgtccatac
     7621 gctctcattc gctaatgctc tgagatccgc gctccgagaa gatcctgaca ttatcctggt
     7681 cggtgaaatg cgggacttgg agaccattca attggcgctg accgcggcag aaaccggcca
     7741 tttggtgttc gcgacactcc acacctccag cgcgcccaag acaattgacc gtatcatcga
     7801 tgcctttccc ccagctcaac aggcccagat tcgcacgcag ctttccgagg ccttggaatc
     7861 ggtgatcaca caaacactgc tcaagaaaaa ggccggcggc cgggtagcgg cgcttgaaat
     7921 catggtggcg acgacggcta ttcgaaactt aattcgcgaa gccaaactcc atcaaattcc
     7981 tgggatcatg caagcgagtc agaaggacgg catgcaaacg atggacatgg cattaatcga
     8041 tttggcaaca cgagggatcg tgaacaaagc tgaggcacaa tcgcgcagca tgaatccgaa
     8101 tttgtttgga gcgaccatgg gtggggccgc ctagcaactt cgacggaagg aaacggatac
     8161 aacatggata ttcgcacgct gttaaaagtc atggtcgatc aggaagcctc ggacatgtat
     8221 ttgaccgtcg acgctcctcc catctatcgt gtgcatgggt caaccgaaca gaccgacgcc
     8281 ccacccttca ccaacgaaca gctcgaagcc cttgccctcg cgctcatgcg cgggcaacaa
     8341 cgt
//
LOCUS       DATDNW010000318         3573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_253775, whole genome shotgun sequence.
ACCESSION   DATDNW010000318 DATDNW010000000
VERSION     DATDNW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253775"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /gene="gcvP"
                     /locus_tag="VI359_07550"
     CDS             complement(<1..1822)
                     /gene="gcvP"
                     /locus_tag="VI359_07550"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY6974144.1"
                     /translation="MSTPDFLDPTDTFIHRHLGPTEADLREMLATAGVPSLEALSDAI
                     VPVDIRLRRELNVPRHRGEQTVLQELRSIAGENRVHRSLLGMGYYDCIVPAVIQRNIL
                     ENPGWYTQYTPYQAEISQGRLEALVNFQTMVTELTGLPLANASLLDEATAAAEAMTMC
                     LAASRMAGRERKTFFVSQDCHPQTIAVVQTRAEPLGITIHVGRTQAIDFSDVQLSGIL
                     LQYPTTDGYVGDYSALVAQAHAAGVLVVVATDLLALTLLRSPGEFGADIAVGSSQRFG
                     VPLGFGGPHAAFLATKEAYRRQMPGRIVGVSKDATGKVAYRLALQTREQHIRREKATS
                     NICTAQVLLAIMASMYAVYHGPEGLRRIAERVHGLAITLASALRQLGFDTGAEQIFDT
                     LHVRLTKERSEQILIRADEQGFNLRRFDDNSIGISLDEWSNEEEIKRLLELFVGHDRL
                     PIRIEEVSRTATVNFSANLARRSPYLTHEVFHRYHTEHEMLRYLHRLQAKDLSLVHSM
                     IPLGSCTMKLNATVEMLPVTWPEFSRLHPFAPLEQSKGYQELFHQLETWLAEITGFAA
                     VSLQPNAGSQGEYTGLMVIRAYHRSQGEFHRDVCLIPVSAH"
     gene            2078..>3573
                     /locus_tag="VI359_07555"
     CDS             2078..>3573
                     /locus_tag="VI359_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY6974145.1"
                     /translation="MKIHDIVIVGAGLAGMRAALASSPDLDVAVISKVHPVRSHSVAA
                     QGGINAALGENDSWEAHAFDTTKGGLFLGDQDAIEVMCREASDDILELERLGVIFSRD
                     DQGRIAQRPFGGAGFPRTCYAADRTGHAILHAMYEQLMKRRIRVYEEWYVTSLIEEDG
                     VCRGVIAWDLIHGGLHTIGAKAVILATGGSGRVFLTSTNAVINTGDGMALAYRAGAPL
                     ADMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKTYAPQQMELATRSTVSL
                     AIGQEILEGRGVDGCVLLDLRHLGRPRILERLPQIRELAIEFAGLDPVETPIPVRPGA
                     HYQMGGVRTNQWGETVIPGLYAAGECACVSVHGANRLGGNSLLETIVFGRRAGRRAGD
                     FARTIAPRALSDTALIQEERRLHQLLANEGPTRAWQLRDELGKTMSLNLGLVRTEASI
                     RSAMATVESLKVRAATVTIQDKGRVFNTDLVQALELQSLLDIAETIVAG"
BASE COUNT          772 a         1066 c         1006 g          729 t
ORIGIN      
        1 cgtgcgcaga aacaggaatt aaacagacgt cgcgatgaaa ctcgccctga ctgcgatgat
       61 aggctcggat caccatcagt cccgtatatt cgccctgaga accagcgttc ggttgcaacg
      121 agaccgcagc gaaccccgta atctccgcga gccaggtttc cagctggtgg aacaattcct
      181 gatacccttt gctctgttcc agcggtgcaa aggggtggag ccgcgagaat tccggccatg
      241 tcaccggcaa catctcgacc gtcgcgttta gcttcatcgt gcaggagcca agcgggatca
      301 tcgaatgaac caacgagaga tccttggcct ggagacgatg gagatacctg agcatttcgt
      361 gttccgtatg atagcgatgg aacacttcgt gggtgagata ggggctcctg cgtgccaggt
      421 tcgccgagaa attcacggtg gccgtccgac tgacctcttc aatgcgaatc ggcagccggt
      481 cgtgacccac gaacagttcc agaagtcgtt tgatctcttc ttcgttactc cattcatcca
      541 gggatattcc aatcgagttg tcgtcgaacc gtcgcagatt gaacccttgc tcgtcggctc
      601 tgattaggat ctgctcagac cgttccttcg tcagccgaac gtgcagcgta tcgaaaatct
      661 gttccgcccc tgtatcgaat ccaagctgac gaagtgcgga ggcgagtgta atcgccaacc
      721 cgtgaacccg ctcggcaatc cgtcgcaacc cctccggccc atgatacacc gcatacatgc
      781 ttgccatgat ggccaggagg acttgcgctg tacagatgtt gctcgttgct ttctcccgac
      841 gaatatgttg ttcgcgtgtc tggagggcca accgataggc gaccttacca gtcgcatctt
      901 tcgacacgcc gacaatacgc cccggcatct gccggcgata tgcctctttg gtcgctagga
      961 acgcggcgtg aggtcccccg aatcccagcg ggactccgaa tcgttggctg gaacccacgg
     1021 cgatatcggc accaaactct cccggtgagc gcaggagcgt cagggcaaga agatccgtcg
     1081 ccaccacgac caacacgccg gcggcgtggg cctgtgcaac aagggcacta tagtctccga
     1141 catacccatc agtcgtcgga tattgcagca ggatgccgct cagctggacg tcagagaaat
     1201 cgatggcctg cgtccgtcca acgtgaatcg tgatgccgag cggttcggca cgggtctgca
     1261 ccaccgcaat ggtctgggga tggcaatcct gtgagacgaa gaaagtcttg cgctcgcgac
     1321 cagccatgcg agaagcggca agacacatcg tcattgcttc cgccgcagca gtggcttcat
     1381 ccagcaatga ggcgttggcc aatggaagtc cggtcagctc agtcaccatg gtttggaaat
     1441 tgaccaaggc ttccaatcgg ccctgagaaa tctccgcctg gtaaggcgtg tattgggtat
     1501 accagcctgg attttcaaga atgttgcgct ggatcacagc gggcacgatg cagtcgtaat
     1561 agcccatgcc gagcaacgag cgatggactc gattctctcc ggcgatggac cgtagttcct
     1621 gcagcacggt ctgctccccg cgatgacgag gcacattcag ctcccgtcgc agccgaatat
     1681 ctactggcac gatggcatcg ctcagggcct ccaacgaggg cacacccgcg gttgccagca
     1741 tctcgcggag atcggcctcg gtcggaccca ggtggcggtg aataaacgta tcggtgggat
     1801 ctaaaaagtc cggcgtactc atttatcctc tattatagct tgaagaggca acgcgggtga
     1861 cacgacgtca gccacaggcc aaccgagtca cgacatgaca tatccgctag cggtcgaaga
     1921 gtaccacgtt agccctccct tctccaagca caagatcggc caccccacac cttgccttcc
     1981 cgacagaaag tcgagtatga gtttggagtc gatgtgaacg aacagaggag attctcttga
     2041 tgtcggttcc tgattgcaac ccaggtcact gctgtctgtg aagatacacg acatcgtcat
     2101 cgtgggggct ggccttgctg gtatgagagc tgcgctcgca tcctcgccgg atcttgacgt
     2161 cgccgtcatc tccaaagtcc atcccgtccg cagccactcg gtggcagccc aaggcggcat
     2221 caatgccgct ctgggtgaaa acgattcctg ggaagcccac gcctttgata ccaccaaggg
     2281 aggtctcttt ctcggcgatc aagacgcaat cgaggttatg tgccgcgaag cgtccgacga
     2341 cattctcgag ttagaacggc tgggggttat tttcagccgg gacgatcagg gccgtattgc
     2401 ccaacgcccc ttcggcggag ccggattccc gcggacctgc tatgccgcag atcgaaccgg
     2461 ccatgcgatc ctccacgcca tgtacgaaca gctcatgaag cgacggatcc gcgtatacga
     2521 agaatggtat gtcacgtcgc tgatcgagga ggacggagtc tgtcgcggcg tcatcgcctg
     2581 ggatctcatt cacgggggcc tccataccat cggggcgaaa gccgtgatcc tcgccacggg
     2641 aggtagcggt cgggtatttc tcacgagcac gaacgcggtc atcaataccg gcgacggcat
     2701 ggccctcgcc tatcgagccg gtgctccgtt ggctgacatg gaattcgttc agttccatcc
     2761 cacgacgttg aaagacaccg gcattctcat caccgaaggg gcgcgcggcg aaggcggtta
     2821 tctgctcaac acactcggcg aacgcttcat gaaaacctac gccccgcagc agatggaact
     2881 cgcgacacgc tctaccgtct cgttggctat cggacaagag attcttgaag gccgcggcgt
     2941 cgatggctgc gtgctcttgg atctgcgcca tctcggacgc ccacgcattc tcgagcgatt
     3001 accgcagatc cgggaattgg ctatcgagtt tgctggtctt gacccggttg aaacacccat
     3061 tcctgtccgc cctggcgctc attatcaaat gggtggcgtc cggaccaatc aatggggcga
     3121 gactgtgatc ccgggtctat atgcagctgg tgaatgtgct tgcgtcagtg tccatggtgc
     3181 gaatcgcctc ggtggaaact cccttctcga aacaattgtc ttcggcaggc gggcaggaag
     3241 acgcgccggt gatttcgcca gaaccatcgc cccgcgcgca ctctcagata ccgcgctcat
     3301 acaggaagag cgacgacttc atcagctcct cgcgaacgaa ggacctacaa gggcctggca
     3361 gcttcgcgac gaactcggga aaacgatgag cctgaacctc ggcctcgtcc gcacagaagc
     3421 atcgatacgg tccgccatgg ctaccgtcga atccctcaag gtccgtgctg ctaccgtgac
     3481 catccaagac aagggacgag tgttcaatac ggatctcgtc caggctcttg aactgcaatc
     3541 attgctggat atcgcggaaa cgatcgtcgc cgg
//
LOCUS       DATDNW010000319        12357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_318847, whole genome shotgun sequence.
ACCESSION   DATDNW010000319 DATDNW010000000
VERSION     DATDNW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318847"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VI359_07560"
     CDS             <1..477
                     /locus_tag="VI359_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY6974146.1"
                     /translation="IAFPATIAGFFETPWVKSIGAQLAPGSLLYTLMYVGLILFFCFF
                     YTAVVLNPVDMADNMKKYGGFIPGIRPGQRTSDYIYKVLTRITFAGAIYLAIVCVIPE
                     FLIYKLNVPFYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMGPLRGRSG"
     gene            487..1143
                     /locus_tag="VI359_07565"
     CDS             487..1143
                     /locus_tag="VI359_07565"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY6974147.1"
                     /translation="MRVVFLGAPGVGKGTQADRVAELFKIAKISTGDLLRGAVRNQTK
                     LGLEAKGYMDQGKLVPDSVVIGLVQEKLSESGCATGFVLDGFPRTTPQADELGRVLGE
                     KRMALDRVVNFEVPREAIVRRLSGRRSCPNCQTTYHVDFSPPKKDGVCDRCGELLVQR
                     SDDRREAVETRLQVYEEQTAPLIRYYADKQLLSHLDASDAVEAVFAKLTKVLEPYRGT
                     "
     gene            1140..1889
                     /gene="map"
                     /locus_tag="VI359_07570"
     CDS             1140..1889
                     /gene="map"
                     /locus_tag="VI359_07570"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY6974148.1"
                     /translation="MIVLKTPDEIEVMAKASRVVAETLEVLKKAVRAGVTTDELDALA
                     EAEILARKAKPAFKGYRNYPKTLCASVNEQVVHGIPSKRKLKEGDIIGLDLGAIVDGF
                     YGDSAVTVMIGQVNDKTAKLVEVTEEALMLGIEQAMVGRRLSDISHAIQRHVEASGYS
                     VVTEFVGHGIGRQLHEEPQVPNYGRPGQGPRLQAGMVLAIEPMVNMGGSAVRVLDDRW
                     TAVTADGSLSAHFEHTIAVQASGPARILSQV"
     gene            1951..2154
                     /gene="infA"
                     /locus_tag="VI359_07575"
     CDS             1951..2154
                     /gene="infA"
                     /locus_tag="VI359_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY6974149.1"
                     /translation="MIEIQGTVAETLPNAMFRVQLDNGHKILAHISGKMRMHFIRILP
                     GDKVTVEMSPYDLTRGRITYRFK"
     gene            2169..2282
                     /gene="rpmJ"
                     /locus_tag="VI359_07580"
     CDS             2169..2282
                     /gene="rpmJ"
                     /locus_tag="VI359_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY6974150.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRILCENPRHKQRQG"
     gene            2401..2796
                     /gene="rpsM"
                     /locus_tag="VI359_07585"
     CDS             2401..2796
                     /gene="rpsM"
                     /locus_tag="VI359_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY6974151.1"
                     /translation="MARIAGVDLPREKRIDIGLTYIFGIGRASAVQILTKAGVDSTIR
                     VKDLSEDKIVKLREIIEQDYQVEGDLRKTFSMNIKRLIDAGTYRGLRHRKGLPVRGQR
                     SKTNARTRKGKRAGVGSKPRPAAKPAPTA"
     gene            2827..3210
                     /gene="rpsK"
                     /locus_tag="VI359_07590"
     CDS             2827..3210
                     /gene="rpsK"
                     /locus_tag="VI359_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY6974152.1"
                     /translation="MSVKKGKRKERRIVQNGVAHVQASFNNTIVTITDMSGNTVVWAS
                     AGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQIDVYVNGPGAGRESAIRSLQAAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            3292..3918
                     /gene="rpsD"
                     /locus_tag="VI359_07595"
     CDS             3292..3918
                     /gene="rpsD"
                     /locus_tag="VI359_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY6974153.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARQRISDYSTQLREKQKLRRIYGLQERQFRGIFERAERQSGVTGDALLQLLECRLDN
                     VTYRLGFGASRKEARQLVGHGHVTVNGRKLNVPGALVKAGDVVEVRERSRTLVPIQAA
                     LEAVDGRGIPEWLELDKATFKGTVRALPTKNQIVLPVNEQLVVELYSR"
     gene            4005..4997
                     /locus_tag="VI359_07600"
     CDS             4005..4997
                     /locus_tag="VI359_07600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY6974154.1"
                     /translation="MIKAMKDFQIPMRVEVDKDTLSPTFGRFTTEAFERGFGTTVGNA
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKGLRLALHTDKPKTI
                     RLKKKGPGEAKGSDIVHDADLTILTPNLHIATLDKDATLDMEMTVKHGRGYAPAERNK
                     EEGLPIGVIAIDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTIAPRDALSTA
                     ASILRDHVDIFINPEERVEGRAEAGTDESQREVNKHLFRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEGEMLKTKNFGKKSLNEIKEILSEMGLTLGAKLDGSAPKNGNP"
     gene            5025..5447
                     /gene="rplQ"
                     /locus_tag="VI359_07605"
     CDS             5025..5447
                     /gene="rplQ"
                     /locus_tag="VI359_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY6974155.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRSLVTSLLEHERIETTEAKAKEI
                     RGFTDRMISLGKQGTLPARRRALSFLRSKEVVAKLFSDVAARFKNRPGGYTRTVKTRQ
                     RIGDAADLVAIELVSRAETTSNKKQAAAAQSPSSEKTG"
     gene            5641..6213
                     /gene="lptC"
                     /locus_tag="VI359_07610"
     CDS             5641..6213
                     /gene="lptC"
                     /locus_tag="VI359_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY6974156.1"
                     /translation="MWQYWAQRSLFAISAILACFLAYLLLTNSTSVPTPTTTMPGSID
                     QADATISNFTFTQTKGATVQWQVQAQQARLFEQDKRALLQTVAVTLFGQQGKELTVTG
                     EEGTLDTATKNFVLANRTTPLVVETTSGYIIYTNHLAWTDHTREIRTQDPVRIVGHGL
                     EVRGRGLLGHLDTEEFEVLEDVHVDLAPAS"
     gene            6188..6769
                     /locus_tag="VI359_07615"
     CDS             6188..6769
                     /locus_tag="VI359_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY6974157.1"
                     /translation="MWIWLLLLNILTFGVPSYGRSEEANLPKGAADQAVSTTITAKKM
                     TVKNHDNQAVFEGTVVLTRGSLVVYSDRMVVLFKPQESAGEDDRHSRDTTKSTTASKG
                     SDVMPTVSNRALNRIEATGRVKIEKESGTATCEKAVYYQSEEKMVLTGNPVAWDKGTR
                     VSGKQITMFLAEDRSVVEGGSHVRIEPDGGGSK"
     gene            6766..7548
                     /gene="lptB"
                     /locus_tag="VI359_07620"
     CDS             6766..7548
                     /gene="lptB"
                     /locus_tag="VI359_07620"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY6974158.1"
                     /translation="MRETVAPNAAPSLVGRESHEVACLSARGLVKGFRGRKVVKGVFV
                     EVRSGEIVGLLGPNGAGKTTIFDMMVGLCQPDEGEITLNGDVITDLPMYKRARKGIGY
                     LPQESSVFRRLSVENNILAILEMLDYSQAERAERVDALLKELDLSHIRTSMAYALSGG
                     ERRRLEITRALATSPSFMLLDEPFAGIDPIAVADIQQIVIRLKEKGIGILITDHNVQE
                     TLSITDRAYIINEGLILESGPPDVIVKSETARAVYLGDRFKL"
     gene            7571..9082
                     /gene="rpoN"
                     /locus_tag="VI359_07625"
     CDS             7571..9082
                     /gene="rpoN"
                     /locus_tag="VI359_07625"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY6974159.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLAQHLMEN
                     PLLDEVPAETQEGEAENADDRKEENSASAGQEQSNEQESTREERDTADEVSASGWEEY
                     FGTDRRMGGGEYASSSQDEFPSYEQTLAKPTSLEDHLVWQLSLSGLSDREKAIGRLLI
                     GNLDDDGYLRTPLAEIVSGTDFSEAEAESVLKDVQSFDPTGVAARDLSECLMLQIGHL
                     GKSPMGSLGARPGALKGSIVEGIVQHHLKDLEKKQYARIAKALDVTVEEVFQATKVIE
                     SLEPKPARPFSNTHNYAIVPDVFVVKKEGEWVVLLNDDGLPRMRISQDYKKLMTSNRD
                     GLGETKAYLEERFRAAQWVIRSIEQRNKTIVKVVSSIVKFQEQFFEHGVQYLKPLVLK
                     QVAEDIGMHESTISRVTANKYMHCPQGMLELKFFFNAGLQRADQPMDMLSSVTVREMI
                     RRMVAEEDARRPLKDEEIAARLRDQQILIARRTVAKYRAEENIPSANQRKRFF"
     gene            9217..9729
                     /gene="raiA"
                     /locus_tag="VI359_07630"
     CDS             9217..9729
                     /gene="raiA"
                     /locus_tag="VI359_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY6974160.1"
                     /translation="MQITGRQLVITPAIRRHVESRLARLDRYDVRIAHITVILGVNKL
                     QHTAEVVCTLQGKRVQAKVSTQEMYASIDQAMARLEAQIRKRKERLVDRKGMRKRVYS
                     SSEHSVSESIDGIDVIRLSPSTLSLDEAKRRLESAPGSLVVFASSTSGKLQILKRLEN
                     GRVALIDPSQ"
     gene            9736..10602
                     /gene="rapZ"
                     /locus_tag="VI359_07635"
     CDS             9736..10602
                     /gene="rapZ"
                     /locus_tag="VI359_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY6974161.1"
                     /translation="MAGLKLVVISGLSGSGKSHALNCFEDAGYFCIDNLPPALLPTFV
                     DLCNQQGGEIKNVGLGIDVRERVFFADLIGILERMKSLGHTVELLFFEAREEVLVRRF
                     SESRRPHPLMPHLPVLEGVRFEKERLQEMRRHADRIIDTSDLTVHDLRELLLRQFRQE
                     SVPRPLTISLLTFGFKFGVPYDVDLLFDVRFLRNPFFVPDLKPLTGEDPRVRNYVLDD
                     PDAIAFIGKLDNLFRFLIPLFERERRSYLNVGIGCTGGRHRSVAIALRLQECFATVGY
                     QVSVIHRDLLKP"
     gene            10956..12065
                     /gene="pilM"
                     /locus_tag="VI359_07640"
     CDS             10956..12065
                     /gene="pilM"
                     /locus_tag="VI359_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY6974162.1"
                     /translation="MLSSLKSLVETDIVSMLTPKRQLVGLDIGSSGIKLVQLKENRGR
                     YVLQKFGVKPLEPEVIVDGTVMDEGRVVSAIRELFEELNVKVKQVAVSISGHAVIIKK
                     ISLPPMPDEELEGQVRLAAEQYIPFDINEVNIDFHVLPPSDGGSDAQGEMSVILVAAK
                     KDKINELTELVKGAGLLPLVMDVDAFAIENMHGINYPVAQDETTALVNIGASVMNVNI
                     VRGGTSLFTRDIPIGGNRYTEAIQRELGMSYEDAEATKKGERSAGANQAAVNTVVDSV
                     NAEVASEIARTIDYFKSTMTDADIQQVLLCGGGSRAKGLVQQLHDRMQVPVEIANPFS
                     EVDTSGSDFDQETLADLGPLAAVGMGLALRSVGDR"
     gene            12062..>12357
                     /locus_tag="VI359_07645"
     CDS             12062..>12357
                     /locus_tag="VI359_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974163.1"
                     /translation="MIRINLLPGPKGRQAKPQYDVRAQALLGVGLVVITLAGCWWYSA
                     ALDTEIEAKQAEKQDKEKQVAQLKEQVKQVADFEQRKKILEDKNRIIDQLEKSRV"
BASE COUNT         3036 a         2889 c         3741 g         2691 t
ORIGIN      
        1 attgcgtttc cggccacgat cgccgggttt tttgaaaccc catgggtgaa gtctatcggg
       61 gcacaactgg cgcctggttc actgctgtac acgctgatgt atgtggggct cattctcttt
      121 ttctgtttct tttatacggc cgtggtcctg aatcctgtgg acatggccga caacatgaag
      181 aagtatgggg gattcattcc aggaattcgc ccagggcaac ggacgtcgga ctatatttac
      241 aaagtgctga cccggattac gttcgcgggg gctatctact tggcgattgt ctgcgtgatc
      301 ccggagttct tgatttataa gttgaacgtt cccttctatt ttggtgggac gtcactcttg
      361 attgtgatcg gtgttgggtt ggatacggct cagcagattg aatcgcacat gctgatgcgc
      421 aattacgaag ggtttcttgg aaaaggcatg ggccccctac gtggtcggag cggttgaggg
      481 acgatcatgc gggtggtgtt tctcggcgca ccgggagtgg ggaaaggcac gcaggccgat
      541 cgagttgccg agctgttcaa gatcgcaaaa atttcaactg gcgatctctt gcgaggagca
      601 gtcagaaatc agacgaagct tgggctcgaa gccaaggggt atatggatca gggaaagctg
      661 gttcctgatt cagtcgtgat cggcttagtg caggaaaagt tgagcgaatc cggttgtgcc
      721 actgggtttg ttttggatgg cttcccgaga accacgccgc aagcggatga gctggggcgg
      781 gtgttagggg aaaaacggat ggccctcgat cgagtggtga actttgaggt tcctcgggag
      841 gcgattgtca ggcggctgag cgggcgacgc agttgcccga attgtcagac gacctaccat
      901 gttgactttt cgccacccaa gaaagatggg gtgtgcgatc gctgtggaga gttgctcgtt
      961 caacgaagtg acgatcggcg agaagctgtc gagacgcggt tacaggtcta cgaagagcaa
     1021 acagctccgc tgattcgata ctatgcagac aagcaactac tctctcattt ggacgcgtcg
     1081 gatgctgtcg aagcggtgtt tgcaaagttg acgaaggtgc ttgagccgta caggggaaca
     1141 tgatcgtcct caagacgcct gacgagattg aagtcatggc caaggcgtcg agggtggtag
     1201 cggaaacgtt ggaggtgctc aagaaggccg tacgggccgg ggtgaccacc gatgaactgg
     1261 atgccctcgc ggaagcggag attctggctc ggaaagccaa gccggcgttt aaagggtatc
     1321 ggaactatcc gaagacgctg tgcgcatcgg tgaacgagca ggtggttcac ggcatcccct
     1381 ccaaaaggaa actcaaggag ggggacataa tcggcctcga tctaggcgcg atcgtcgatg
     1441 ggttctacgg cgactcggcg gtgacggtga tgatcggtca ggtgaatgac aaaaccgcca
     1501 agttagtcga ggtgacggaa gaggccttaa tgctcggcat tgaacaggcg atggtaggcc
     1561 gccgtctctc ggatatctca catgcgattc agcgccatgt ggaggcttcc gggtattctg
     1621 ttgtgacgga gtttgtcggt catgggattg gtcgccaact acacgaagag ccgcaagtgc
     1681 cgaactacgg caggcccgga caggggccgc gactacaggc agggatggtc cttgcgattg
     1741 agccgatggt gaatatgggt gggagcgcgg tcagggtgct cgatgatcgg tggacagcgg
     1801 tgacggcaga cggaagtctc tcggctcatt ttgaacatac gatcgcggtt caggcgagcg
     1861 ggccagccag aatattgagc caggtgtaaa gggggattcg gattggccgt taggcgaaag
     1921 aaaggataac gattcgtgcc gaaggaagat atgatcgaga tacaaggcac ggtcgccgaa
     1981 acgttaccga acgcgatgtt ccgcgtccaa ctcgataatg gccacaaaat tcttgcacat
     2041 atttccggga aaatgcggat gcatttcatt cgcatcctgc ccggggacaa ggtcacggtt
     2101 gagatgtcgc cctatgatct aacaagaggg cgcatcacat atcggtttaa gtaggagtgt
     2161 agacggctat gaaggtcaaa tcgtcggtga agccgatttg tgccaaatgt aaagtggtga
     2221 ggcggcgagg cgttgtcagg atcctctgcg aaaatcctcg gcataagcag aggcaggggt
     2281 gagcaggacg ccccgaccag tctcctgctc gcgcaacgcg cggcctcgga aggccctcgt
     2341 tggacgcgcg catgagagcc tgatcgggcc atcctgcagg aagtagaagg gagaacaaag
     2401 atggcacgga tagcaggggt ggatttgcca agagagaagc gaatcgacat cggtctgacc
     2461 tatatttttg gaatcggacg ggcgtcggcg gtgcagattt tgacgaaggc cggcgtggat
     2521 agcacgattc gggtaaagga tttgagcgaa gacaagattg tgaagctccg tgaaatcatc
     2581 gaacaggatt atcaagttga aggcgatctt cggaaaacgt tctcgatgaa tatcaagcgt
     2641 ctgatcgacg ccgggacgta ccgaggtttg cggcaccgca aggggcttcc ggttcgtgga
     2701 cagcggtcaa agaccaatgc gcgcacaaga aaaggcaagc gagcaggggt gggtagcaag
     2761 ccaaggccgg cagcaaaacc agcgccaacc gcctgacatc tcacacgtcg tcacgaggga
     2821 gtttgtatga gcgtcaaaaa agggaagagg aaagaacgcc gtatcgtgca gaacggggtc
     2881 gcacatgtgc aagcgtcgtt caacaatacg attgtgacga ttaccgacat gagcggcaat
     2941 acagtggttt gggcaagtgc ggggaaccag gggtttaaag ggtcccgcaa aagcacccct
     3001 tttgcggcgc agcgagccgg tgaagcagca gctcgtaaag cgatggaaag tggcatgcga
     3061 cagatcgatg tctatgtgaa cgggccggga gctggccgcg agtcggcaat ccgctctctg
     3121 caagcagcgg gattacggat caatttgatt cgcgatgtga cgccgattcc acacaacggg
     3181 tgccgtcccc cgaagcggcg gcgcgtctaa tgttgaagag cttaggtgta gtcgtttacc
     3241 agaattcgtg ggcacatcct gacgatgtgt ccgataaccg gaggtagggg cgtggcaaag
     3301 tatcgtggtc ctgtatgtcg actctgtcgt cgggaaggtg aaaagctgtt tttgaaaggc
     3361 tcgcgctgta tgacggagaa atgcgcaatc gagcgtcgga gctatcctcc gggacaacat
     3421 ggtcaagctc gtcagcggat ttcggattac agcacgcagt tacgcgaaaa acagaagctg
     3481 cggcgcattt atggccttca ggaacggcag ttccgcggca tctttgagcg cgccgagcgg
     3541 caatctggtg tcacgggtga cgcgctgctt caactactcg aatgccggct cgacaatgtc
     3601 acgtaccgct tgggatttgg agcgtcgcgc aaagaggctc gccagctcgt gggtcatggg
     3661 cacgtcacgg taaatggccg caagctgaat gttccgggag cgttggtgaa ggccggcgat
     3721 gtcgtcgagg tgcgggaacg aagtcgaacg ctggtcccga tccaggctgc gctggaagcg
     3781 gtggacggac gcgggattcc cgaatggctg gaactggaca aggcgacctt caaagggacc
     3841 gtgagagcac tccccacgaa gaatcagatt gttcttccgg tcaacgagca attggtcgtg
     3901 gaattatatt cgaggtaggg cagcgcttcg agagagatag tggctgtcaa tagaggcgat
     3961 agatactcga tgaccgagct actcgatgag atgaaggggg agtcatgatc aaagcaatga
     4021 aagactttca gatcccgatg cgggtggaag tcgacaaaga tacactttct ccgaccttcg
     4081 gtcgatttac caccgaagcg tttgaacggg gattcggcac gacagtcggc aatgcattgc
     4141 ggcgtgtgct gttgtcctcg ttgactggcg cggcggtgac gacggttaaa atcgaagggg
     4201 tgctgcacga attttccacg atccccggtg tgacagaaga tgtcacgtcg atcatcctga
     4261 acgtcaaagg tttacgcctg gcgttgcata ccgataaacc taaaacgatc cggttaaaaa
     4321 agaagggacc aggtgaggcc aaagggtctg acatcgtcca cgatgccgac cttactattc
     4381 tgacgccgaa tctccatatc gccacgttgg acaaagacgc cacattggat atggaaatga
     4441 ccgtgaagca tggacgcgga tatgctccag ctgagcggaa caaggaggag gggctcccga
     4501 tcggggttat cgcgatcgat tctattttct caccgattaa acgggtcaat tttcacgtgg
     4561 agaatgcccg cgtcgggcgc atgactgatt acgacaagtt gacgttagaa atttggacgg
     4621 atggcacgat cgccccgcgc gatgcgctgt ctaccgctgc gagcatcttg cgcgaccatg
     4681 tagatatctt tatcaatcca gaggagcgtg tcgaaggacg tgctgaggcg gggactgatg
     4741 agtctcagcg cgaagtcaat aaacatctgt tccggagcgt gaacgaactg gaattgtcgg
     4801 tgcgcgcagc aaattgcttg aagaacgcga atatcaagac gattgcggat ttggtgcaga
     4861 agaccgaagg cgagatgttg aagaccaaaa atttcgggaa aaaatcgctg aatgagatca
     4921 aggaaatttt gtcggagatg gggttgactc tcggagccaa gctggatggc tccgctccga
     4981 aaaatggcaa tccataatct aacgagcaac acaagaggat acctgtgcgt caccgaaaaa
     5041 agggcagaca gctcgggcgg cagacgaaac atcggtgggc tttgttcaga agcttggtca
     5101 cctccctcct tgaacatgag cggattgaga cgaccgaggc gaaggccaaa gaaatccgag
     5161 gctttacgga tcggatgatt tccttgggaa agcaaggcac gttaccggcc agacggcgag
     5221 cattgagttt cctccggagt aaggaggtcg tggccaaact gttcagtgat gtggcggcac
     5281 gcttcaagaa tcgtcccggc gggtatacac ggacggtaaa gactcgccaa cggatcggag
     5341 acgcagctga cctggttgcg atcgaactcg tatcgagagc ggagaccacg tcgaacaaga
     5401 aacaggctgc agctgctcag tccccttcaa gtgagaagac cggttaggtt tccaatccct
     5461 tgtgcggcat accggtggtg cgttctggcc cgaccatcct cgttgctcgc gtatcgatag
     5521 cccagcttca agtgattttc ctcttttgaa aagttgtcat gacagcggca tacgtttact
     5581 tgcttcatgg cacatgctat tatcgtgcca ggcattgttg aatgagggat accaagtact
     5641 gtgtggcaat actgggccca acgaagtttg ttcgcaatta gcgcgattct agcttgcttt
     5701 ctggcatacc tgctcctgac gaactccaca tcagttccta ctccaaccac gaccatgcct
     5761 ggatcaattg atcaggctga tgccacgatc tcgaatttta cgtttactca aacgaagggt
     5821 gcgactgttc aatggcaagt tcaggcccaa caagcgcggc tttttgaaca ggacaagcga
     5881 gccctgctcc agacagtggc tgtgaccctg tttggtcagc aagggaagga actgaccgtg
     5941 accggagagg aagggacgct ggataccgcc acgaaaaact ttgtgctcgc aaatcgaact
     6001 acgccgctgg tcgtagaaac gacgagtgga tacatcattt acacgaatca ccttgcatgg
     6061 actgatcaca cgcgagagat ccgtacgcag gatccggtgc gcattgtcgg tcatggattg
     6121 gaagtcagag ggcgtgggct cttgggacat ctggatacgg aagagtttga ggtacttgag
     6181 gacgtccatg tggatctggc tcctgcttct taacattctg acattcggtg ttccctccta
     6241 tggtcgatcg gaggaagcca accttccaaa aggagcggcc gatcaggcgg tcagcacaac
     6301 cattactgcg aagaaaatga ctgtcaagaa tcacgataac caagcagtct ttgaggggac
     6361 ggttgtgctc acgcgcgggt cactggttgt gtattccgat cggatggtag ttctgtttaa
     6421 gcctcaagag tccgctgggg aagatgatcg ccattcgcgg gatacgacaa agagcacgac
     6481 ggcttcaaag gggtcggatg taatgccgac cgtctcgaat cgagctttga atcgaatcga
     6541 ggcgaccggg agggtaaaga ttgaaaaaga gtctgggact gcgacctgtg agaaggcggt
     6601 gtactatcag agcgaagaga aaatggtgct gacagggaac cccgttgcat gggacaaagg
     6661 taccagagtg agtgggaaac agatcacgat gtttttggca gaagatcgaa gtgttgtcga
     6721 aggtggttcc catgttcgca tcgaaccgga tggaggaggc agtaagtgag agagaccgtt
     6781 gcgcccaacg ccgcgccgag tctcgttggg agagaaagcc acgaggtcgc ctgcttgagc
     6841 gcaagaggac tagtcaaagg gtttcgcggg cgaaaagtcg tgaagggggt cttcgttgaa
     6901 gtcagatccg gagaaatcgt cgggctcttg gggccaaatg gggcaggtaa gacgaccatc
     6961 ttcgatatga tggtgggcct ctgccaaccc gatgaaggag agatcaccct caatggggat
     7021 gtcatcacgg atctgccgat gtataaacgg gctcggaagg gcatcgggta cttgcctcag
     7081 gaatcttcgg tgttccggcg cctgtccgta gaaaacaata ttctcgcaat tctagaaatg
     7141 ctggattact ctcaggccga acgggccgag cgtgttgatg cactactgaa agaactcgac
     7201 ttgagtcata ttcggaccag tatggcctat gccttgtcag gcggtgagcg gagacggttg
     7261 gagatcacgc gcgcactggc gacaagcccc tccttcatgc ttttggatga accgttcgca
     7321 ggaatcgacc cgatcgccgt tgctgacatt caacaaatcg tcattcggct gaaagaaaaa
     7381 ggcatcggaa tcctgattac cgaccataac gtgcaagaaa ccctatcaat aaccgacagg
     7441 gcctatatca tcaacgaagg cctgatcctg gaatcggggc ctcccgatgt tattgtaaaa
     7501 agtgaaaccg cgcgggcagt atatttagga gaccggttca agctctagcc gatcaattct
     7561 gtggagtgcc atgaaactga gactggatct caggcttagc caaaagctga tcatgacccc
     7621 gcaattgcag caggcgatca agctgctgca gttgtcccgt ttagagctcc agcagagcct
     7681 tgcgcagcat ttgatggaaa atcctctcct cgatgaagtg ccggctgaga cgcaagaagg
     7741 tgaagccgaa aacgccgatg atcggaaaga ggaaaattcg gcgtcagcgg gacaagaaca
     7801 atcgaatgaa caagaatcca ctcgggagga acgcgacacg gctgatgaag tttcggcatc
     7861 cggatgggag gagtattttg gaaccgaccg tcgaatggga ggtggggaat atgcctcgtc
     7921 gtcgcaggat gagtttcctt cctatgagca gactctagcc aaacctacct cgctggaaga
     7981 tcacttagtc tggcaactgt cactttctgg cttgtctgat cgcgagaaag caatcggtcg
     8041 gttgctgatc ggaaacctcg acgacgatgg gtatctccgc acgccgttgg ccgagatcgt
     8101 tagtggaacg gattttagtg aagcggaggc agaatcagtt ctcaaggatg tgcagagctt
     8161 cgatccgacc ggggtggctg caagggacct gtctgaatgt ctgatgttgc agatcggcca
     8221 tttgggcaag agtcccatgg ggtcgctcgg tgcccgtcct ggggcgctga aggggtcgat
     8281 tgtcgaaggc atcgttcaac accatctgaa ggacttggag aaaaaacaat atgctcggat
     8341 cgcgaaagcg ttagatgtga cggttgaaga ggtgtttcag gcgaccaagg tcatcgaatc
     8401 acttgagcct aagccggctc gaccgttttc caatacgcac aattatgcca ttgtgcctga
     8461 cgtgtttgtc gtgaagaagg agggggagtg ggttgtcttg ctgaacgatg atggactgcc
     8521 gcgcatgcgg atcagccagg attataaaaa actcatgacg tcgaatcgcg acggattggg
     8581 tgagacgaaa gcctatctcg aagagagatt tcgggcagct caatgggtca ttcgcagcat
     8641 cgagcagcgg aacaagacga ttgtgaaggt ggtatcgagc atcgtcaagt ttcaggagca
     8701 gttttttgag cacggggtac aatatctcaa accacttgtt ctgaagcagg tcgcagagga
     8761 catcggcatg cacgagtcta cgatcagccg tgtcacggcc aataaatata tgcattgccc
     8821 acaaggtatg cttgaactga agttcttttt taacgcggga cttcagcggg ctgatcaacc
     8881 gatggatatg ttatcttcag ttacggttcg agaaatgatt cgaagaatgg tggctgaaga
     8941 ggacgctcgg cgaccgttga aggacgaaga gatcgccgcg cggttgcgcg atcagcaaat
     9001 tctgatcgct cgacggaccg tggccaaata tcgggccgaa gagaacattc cctctgcgaa
     9061 ccaacgcaaa cggttctttt gaaccagcag tccccgtgca cagtttctac ctatcatcgg
     9121 gcaagtcttt ctggtcagga gcaggctcat tgtggagtcc tgcgcaggcc agatctgaca
     9181 attgggcatc tgcagcaagc gacaggagct gaggttatgc aaatcactgg gagacagctt
     9241 gtcatcacac cggctatacg acgtcatgtg gaatccaggt tggctcgact agatcgatat
     9301 gacgtgcgga tcgcacatat caccgtgatt ctcggagtaa acaagctcca gcatacggca
     9361 gaagtcgtat gcacgctcca agggaaacgt gtgcaagcaa aagtgtcaac gcaggaaatg
     9421 tatgcctcca ttgatcaggc tatggctcga ctggaggcgc aaatccgaaa gcgcaaggaa
     9481 cgactcgttg atcgcaaggg catgaggaag cgagtgtatt cgagtagtga gcatagtgta
     9541 tcggagagta ttgacggcat tgatgtgata cgtctctcgc cctcaacgct ctcgctcgat
     9601 gaggcgaaga ggcggcttga atcagctcct ggctctctcg tggtttttgc aagttcgaca
     9661 tccgggaaac tgcaaatctt aaagcgtctc gaaaacggcc gcgtggcctt gattgatccc
     9721 tcgcagtgaa tcctcatggc aggactcaag ctcgtcgtta tcagcggtct ctcgggttcg
     9781 ggaaagtctc atgccctcaa ttgcttcgag gacgcgggat atttctgcat tgataacctc
     9841 cccccagctt tgctcccgac tttcgtggat ctgtgcaacc agcagggcgg cgaaatcaaa
     9901 aatgtgggcc tgggaatcga tgtgcgggag cgggtgttct ttgccgatct catcggcata
     9961 ctggagcgca tgaagtcgct tggtcacacg gtcgaattat tgttttttga agccagagag
    10021 gaggtgctgg tgcgtcgctt ttccgaatct cgccgtcctc atccgctcat gccccacctc
    10081 ccagtgttag aaggtgtacg gtttgaaaaa gagcgtcttc aggaaatgcg ccggcacgcc
    10141 gatcggatta ttgatacgtc ggacctgacg gtgcacgact tgcgggaact tttgctaaga
    10201 cagtttcggc aggagtcagt cccaagaccg ctgaccattt ccttgctcac atttgggttc
    10261 aagtttggtg tgccgtatga tgtggacctt ctcttcgacg ttcgctttct tcgcaatcct
    10321 ttcttcgtgc cggatctgaa gcctctgaca ggagaggatc caagagtacg gaattatgtg
    10381 ctcgacgatc ctgacgcgat cgcatttatc gggaaattgg ataatttatt ccggtttctg
    10441 attcccctct ttgagcgcga acgccgcagc tatttaaacg tcggtatcgg ctgcactgga
    10501 gggcgacatc gatcggttgc gatcgctctg cgtctccaag aatgcttcgc tacggtggga
    10561 tatcaggtta gcgtgatcca ccgcgatctc ctcaaaccat agtcctgcga taaccagcag
    10621 cctcgcctcc gacaggcttc cctcagtttc ttgacagctt tcatgcatga actatacttc
    10681 ccttatgtat cgcagtggtc tgcaacggtc acgctagatt ggatgcaggc caatgccaaa
    10741 cagggcctac cactgcggat cgtcctgggg cagcgtttga gacacccata taaataggtt
    10801 tccgcaatgg aaagtgaata gcggtgaata accagttcgt agaatggggt ttggttagtt
    10861 ggtcggttga gtatggaagg cgagagcaat aaccggtacg aaagtacgaa aaagatttgc
    10921 catttaccgt tagtagtgta cggttcgagg tacctatgct gagttcactt aagagtttgg
    10981 ttgaaacgga tatcgtctcg atgctgacgc cgaaacgtca gctagtcggg ctcgacatcg
    11041 gatcgagcgg aatcaagctg gttcagctga aggagaatcg tggacggtac gtgctgcaga
    11101 aattcggggt aaagccgctt gagccggaag tgatcgtcga cggaaccgtg atggatgaag
    11161 gccgggtagt ttcagccatt cgtgagttgt ttgaagagct taatgtgaaa gtgaagcagg
    11221 tggcagtgtc tatctccggt cacgcggtca ttattaagaa gatcagtttg cctcccatgc
    11281 cggatgagga attggaaggc caggtgaggc tcgcggcaga acagtacatt ccattcgata
    11341 tcaatgaggt caacatcgat tttcatgtcc tgcccccatc tgacggaggc agcgacgctc
    11401 aaggcgagat gtcggtcatc ttagtcgcag caaagaagga taaaatcaac gaattgacgg
    11461 agcttgtgaa gggggcgggg ctgctaccat tggtcatgga tgtcgatgct tttgccatcg
    11521 agaacatgca cgggattaac taccccgttg cgcaggacga gacgaccgcg ctcgtcaaca
    11581 ttggcgccag cgtgatgaac gtcaacattg tccgaggggg gacgtctctg tttacgcgtg
    11641 atattccgat tggtgggaat cgatatacgg aggccatcca acgggaatta ggaatgtcct
    11701 atgaggatgc ggaggccaca aaaaagggcg aacgttcggc aggggcgaac caagcggcgg
    11761 tgaatactgt cgtcgacagt gtgaatgccg aggtcgcatc agaaatcgca aggacgatcg
    11821 actactttaa atcaaccatg acggatgccg acattcaaca ggtccttttg tgcggaggag
    11881 gctcgcgagc caagggactg gttcagcagc ttcatgaccg catgcaggtt cctgtcgaga
    11941 ttgccaatcc cttcagtgaa gtggatacct cagggagtga ttttgatcag gagaccttag
    12001 ccgacctcgg acctcttgcg gcagtgggta tgggcctcgc gctcagatcg gtaggcgacc
    12061 gatgatccga attaacctat tgcctgggcc caaagggcgc caggcaaagc cacaatatga
    12121 tgtcagagct caggctttac tgggtgtcgg actcgtggtg attacgctag caggatgctg
    12181 gtggtattcc gcggcactcg atacggagat tgaagccaaa caggcggaaa aacaggataa
    12241 ggaaaagcag gtcgcacagc tcaaagaaca ggtcaagcaa gttgcggact tcgaacaacg
    12301 caagaagata ttggaagata agaatcgcat catcgaccag ctggagaaat cacgcgt
//
LOCUS       DATDNW010000320         4046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1205125, whole genome shotgun sequence.
ACCESSION   DATDNW010000320 DATDNW010000000
VERSION     DATDNW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205125"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VI359_07650"
     CDS             complement(<1..471)
                     /locus_tag="VI359_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974164.1"
                     /translation="MSLAALAVDLSWSTPSGLARSNLTTLVLQYLDVPEADQAERTLQ
                     DIMADPDASIDTITRLIQTGREYGSQPIGTLPDEQITLQGRTYHLALFVPQTYQPGKG
                     YGLVVCLHGAGFSGDAYLERWQARLGEDYVLACPTYPAGAWFTRRAEDLVLATIH"
     gene            complement(498..1610)
                     /gene="ribD"
                     /locus_tag="VI359_07655"
     CDS             complement(498..1610)
                     /gene="ribD"
                     /locus_tag="VI359_07655"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY6974165.1"
                     /translation="MTTAAQDLRFMTVALRLAAKGRGKTSPNPMVGALVVAKGRIVGR
                     GYHHGPGQPHAEILALQEAGPRAARATLYVTLEPCSHLNKRTPPCIPAVIESGVRRVV
                     VAMVDPNPSVKGRGLAQLRRAGVIVTVGVARREAAHLNRTYIHWVETGRPYVILKAGM
                     TLDGKIATARGESRWITGAKARQEVHQLRRTVDAILVGSGTVMKDDPSLTARISEHPL
                     KLATRQPLRVVADSRLRVSPTAKVLSKHARTLIATTTNVSRAKVGLLQQRGIEVAMLP
                     SVRGRLVLRALLAYLGKNGVTSVLIEGGSTLNAAALRSKLVNHVVLYMAPTLMGGQDA
                     RAVIGDRSPGRLAQSMKLRNVTVRRLGDDVVVEGDL"
     gene            complement(1607..>4046)
                     /gene="ppdK"
                     /locus_tag="VI359_07660"
     CDS             complement(1607..>4046)
                     /gene="ppdK"
                     /locus_tag="VI359_07660"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY6974166.1"
                     /translation="EGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAILKHKK
                     TEVGVAHETHLDARHLRELVASFKALVKEETKKDFPDEPLDQLRMAINAVFSSWFGAR
                     AKTYRQLYGIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRNPSTGDKVFFGECLMNAQ
                     GEDVVAGIRTPLPVSALAKNVPEAYKDLEHTYKKLEKHYRDMLDLEFTIQEGKLYMLQ
                     TRVGKRTGIAAVKIAVDLVNEGAISKHEAVQRIGPDQLAQYLYPIFDTKEESKVNPLG
                     KGLPAGPGAAAGKIALTPDRAVDMKAAGQRVILVRQETSPDDIHGMNAAAGFLTARGG
                     MTSHAAVVARQMGKVCVAGCEAIEVVDAQSVRIGAKVFREGDYISVNGSTGNVYEGDI
                     PVVESEIIQVIQGKLNPSKSDKYQRFSTILSWADDIRKLRVRANADVPEQARIAKGFG
                     AEGIGLCRTEHMFFAEDRIPLMQKMILARTKEEREKYLDQLLPLQKQDFIGLYREMKG
                     YPVTIRLLDPPLHEFLPKREDLMVEIARLELTGNDGALLDEKRRLLVRVEELHEFNPM
                     LGLRGCRLGITMPEITRMQARAIMEAACEVAKEGKKIVPEIMIPLVGMVSEMRSQKDL
                     VREAAQETMKRNNVKLSYLVGTMIELPRAAVTADRIADEAEFFSFGTNDLTQTTFGFS
                     RDDAAKFIDFYRTANIMDADPFAVLDREGVGSLMKQAIVAGRTTRPTIKLGICGEHGG
                     DPSSVEFCHQIGLDYVSCSPYRVAIARLAAAHAALAESEASKSTVTSSKSRSKSTGSG
                     RSPHRRSRVVRKKR"
BASE COUNT          824 a         1255 c         1085 g          882 t
ORIGIN      
        1 atggatcgtc gccagcacta ggtcttccgc ccgtctggtg aaccaggctc ccgccgggta
       61 tgtgggacag gcgagaacat aatcctcccc gagtctcgcc tgccaccgtt ccaaataagc
      121 atcgccggaa aatccggctc cgtgcaaaca cacaaccaaa ccataccctt tacccggctg
      181 ataagtctgc ggcacgaaca gtgcgaggtg ataggtgcgc ccctgcaacg tgatctgctc
      241 gtccggcaat gtcccaatcg gctgagaccc atactcgcgc cccgtttgga tcagacgtgt
      301 gatggtgtca atcgaggcat ccgggtcggc catgatgtct tgcaacgtcc gttcagcctg
      361 atctgcctcc ggcacgtcaa gatactggag cacaagcgta gtgagattgg agcgagccag
      421 cccggatggg gtgctccaac tgaggtcgac tgcgagcgct gccaggctca tgagcgcgcc
      481 gatgatggat ttgatcttca gagatcaccc tccacaacga catcatcccc gagacgacgt
      541 acggtgacat tccgtaactt catcgattgg gccaatcgcc cggggctccg atcgccgatc
      601 acggcacgtg catcctggcc gcccataagt gttggcgcca tataaaggac gacgtgattg
      661 actaacttcg accgtaatgc cgctgcattg agcgtactcc caccttcgat gagaactgac
      721 gtcacgccgt tctttccaag ataagccaac aaggcccgga ggaccagacg accgcgaacc
      781 gacggaagca tcgcaacctc aatccctcgt tgttggagca gacccacttt ggcccgagaa
      841 acgttggttg ttgtggcgat caacgtccgc gcatgcttcg aaagaacttt ggctgtggga
      901 gacacacgca ggcggctgtc ggcaacaacg cgcaacggct gacgcgtggc aagcttgagg
      961 ggatgctccg atatgcgggc ggtcaatgaa ggatcgtcct tcatgaccgt gccgctccca
     1021 accagaattg catcgacggt tcgtctgagc tgatgcacct cttgtcgcgc cttggcaccc
     1081 gtgatccatc gcgattctcc ccgagccgta gcaatctttc catccaacgt cattcccgct
     1141 ttcagaataa cgtagggacg acctgtctca acccaatgga tataggttcg attaaggtgc
     1201 gccgcttccc gccgtgccac accaaccgtc acgataaccc cggcacgtcg aagctgggcc
     1261 aatccccgcc ccttgacgga tggattggga tcgaccatgg ccacgacgac acggcgcaca
     1321 ccggattcga tcactgccgg gatacagggc ggagtccgtt tgttgagatg gctgcagggt
     1381 tcaagcgtca catagagggt cgctcgggcg gcgcgcggtc cggcttcctg aagcgcgaga
     1441 atttccgcgt ggggttgccc aggcccgtga tgatagccgc ggccgacgat gcgccccttt
     1501 gccacgacga gtgcgccgac catcggattc gggctggtct tcccgcgtcc cttcgcagcc
     1561 agacgaagag ccacggtcat gaagcggagg tcctgtgcgg cggtggtcac cgtttcttgc
     1621 gaacgacccg tgaacggcgg tgcggagatc gtcccgatcc cgttgatttc gatctggact
     1681 tggagcttgt gaccgtcgac ttgctcgcct cgctttcggc cagagccgcg tgggcggcag
     1741 cgagccttgc gatcgccact cgataagggg aacagctcac atagtccaat ccaatctgat
     1801 ggcagaactc cacggaactc ggatcgccgc cgtgttcccc gcagataccg agcttgatcg
     1861 ttggacgagt cgtacgaccc gctacaatcg cctgcttcat caaggagcca accccctcac
     1921 gatccagcac ggcaaaggga tcagcatcca taatattggc cgttcgatag aaatcgataa
     1981 acttggccgc gtcatcgcgg gaaaatccga acgtcgtttg cgtgaggtcg ttcgtaccga
     2041 atgagaaaaa ctcggcctca tcagcgatgc gatcggccgt cacggcggcg cgtggcaatt
     2101 cgatcatcgt gccaaccaga taggagagct tcacattgtt tctcttcatg gtctcctgag
     2161 ccgcttcgcg cacgagatcc ttctgcgacc tcatttccga gaccatcccg acgagtggga
     2221 tcataatttc cggcacgatc ttcttgcctt ctttcgccac ttcacaggcc gcttccatga
     2281 tggcacgggc ctgcatgcgc gtgatctctg gcatggtaat ccccagtcgg catccgcgga
     2341 gcccgagcat cggattgaac tcgtgcaact cttccactcg caccaacagt ctgcgtttct
     2401 catccagcaa cgcaccatcg ttgcccgtca attcgagtcg ggcaatctcc accatgagat
     2461 cttcgcgctt cgggagaaat tcatggagcg gcggatcgag caggcgaatc gtgacgggat
     2521 agcctttcat ttctctgtag agcccgatga aatcctgctt ctgcagcggc aataactggt
     2581 cgagatattt ttctcgctct tccttcgtcc gcgccagaat catcttctgc atgaggggga
     2641 tgcgatcctc agcaaagaac atatgttccg tccgacacaa cccgatccct tcggccccga
     2701 agcccttggc gattcgcgct tgttctggca cgtccgcatt cgcccgcacc cgcaactttc
     2761 gaatgtcgtc ggcccaggac agaattgtcg agaatcgctg atacttgtcc gacttgctcg
     2821 gattcaactt cccttggatg acttgaatga tttcggactc gaccaccggg atgtcgcctt
     2881 catacacgtt cccggtcgag ccattgaccg aaatgtaatc cccttcgcga aagaccttgg
     2941 cgccgattcg aacggattgc gcgtcaacga cttcgatcgc ttcgcacccc gccacgcaga
     3001 ccttgcccat ctggcgcgcc acgacagccg catgggacgt catgcccccc cgtgcggtta
     3061 gaaatcctgc ggcggcgttc atgccgtgaa tatcgtctgg gctcgtttcc tgccggacca
     3121 aaatgacgcg ctgcccggca gccttcatgt cgacagcacg atccggtgtc agcgcgattt
     3181 tcccagctgc agcacctgga ccagccggga gacctttccc gagtggattg actttggatt
     3241 cttccttcgt atcgaagata ggatagagat attgtgctaa ctggtcggga ccgatccgct
     3301 gcaccgcctc gtgtttagaa atcgctccct cgttcaccaa atcgaccgcg atcttgacgg
     3361 ccgcgatgcc ggttcgtttg ccgacccgcg tttggagcat gtagagcttg ccttcttgaa
     3421 tcgtaaactc gagatcgagc atgtcgcgat aatgtttttc aagtttcttg taggtgtgct
     3481 cgagatcctt gtaagcctcc ggcacgttct tggcgagcgc gctcaccggc agcggggtac
     3541 gaatccctgc aacaacgtcc tctccttgtg cattcatcag acactccccg aaaaacacct
     3601 tgtctcccgt actggggttt ctcgtgaatg cgacgcccgt gccgctcgtt tctcccatgt
     3661 tgccgaacac cattgccacg acattgaccg ccgtgcccca cgaatcggga atcccataga
     3721 gttgacgata ggttttggca cgcgcgccga accaggacga gaagacggca ttgatcgcca
     3781 tacgcagttg gtcaagcggt tcgtcgggaa aatccttctt ggtctcttcc ttcaccaacg
     3841 ctttgaagct cgccaccaat tcacggaggt ggcgtgcatc caaatgcgtt tcatgcgcca
     3901 caccaacttc ggtcttcttg tgtttgagga tcgcctcgaa atgctcgcgc gggactccca
     3961 tgacgatact gccgaacatt gagacaaagc ggcggtagct gtcctgagca aaccgatcat
     4021 tccgcgtttt tgccgccaat ccttcg
//
LOCUS       DATDNW010000321         2994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1145418, whole genome shotgun sequence.
ACCESSION   DATDNW010000321 DATDNW010000000
VERSION     DATDNW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145418"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VI359_07665"
     CDS             complement(<1..180)
                     /locus_tag="VI359_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY6974167.1"
                     /translation="MKTLATLEPLTTKLLEIQQINSAASVLSWDQETYMPAGGGSARA
                     DQIATLQGIAHQKFVS"
     gene            339..995
                     /locus_tag="VI359_07670"
     CDS             339..995
                     /locus_tag="VI359_07670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974168.1"
                     /translation="MKELVPAEIEMYAQAHSMPESDLCRELRAETTRRMEFPQMVVGP
                     LEGAFLKMMTQIVGAKRVLEIGMFTGYSALCFAEALPKDGTILTCEVDDESAALARAY
                     FARSPHGRKIEIRMGPALDTMRSLTGPFDLIFIDADKTNYVNYYRRALDLISPNGVIL
                     VDNVLWDGEVLLQPPPDERTAAIQELNRVVAADSRVTAVLLTIRDGVLMIRPNVNVSR
                     "
     gene            992..1897
                     /locus_tag="VI359_07675"
     CDS             992..1897
                     /locus_tag="VI359_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY6974169.1"
                     /translation="MSQPTDMGAYGALVTAAVLWGGSIVAQKLALGSFSAVEASVLRD
                     IGGLIILLATWWWQDGTFAKLTKADAQTLCWLGLCVLGNHLLILIGLRYVSGAVAGVI
                     IGSSPVVTSLLSAILIQDVPLRTVWMGAVLSCAGVGLVSVAGFSAAGDQPLLGGSLVF
                     LGVVSWALYSIGSRSIMERVPALTVNWTTLLVATALQLPLLWTDRKMLEAGPASVATP
                     DWFALGYLVVFATAVAQQAWLFGVKGVGPSRASVLGNLTPVAAVGLSALILHESVGAV
                     EVIGIVLILAGVWVVHQQTSRLSPS"
     gene            2100..2492
                     /locus_tag="VI359_07680"
     CDS             2100..2492
                     /locus_tag="VI359_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6974170.1"
                     /translation="MIERRIEEVTKANQRFYEAFESLDIAKMDEVWAHLEYVTCVHPG
                     WTLRSDWPAVRDSWVLIFNNTFSMKFELTEVMVQVAGDMAWVICVENITSQQSDEPQQ
                     AKVLATNLFERIGDEWLMIHHHGSAVMG"
BASE COUNT          588 a          795 c          946 g          665 t
ORIGIN      
        1 agacacaaac ttctgatgtg caattccttg aagcgtcgca atctggtcgg cgcgggcgct
       61 cccgccgccg gcaggcatat acgtttcctg atcccacgac aggaccgacg cggcactatt
      121 gatctgctga atctcaagta attttgtcgt caggggttcc agtgttgcca atgtcttcac
      181 gcgcagtctc ctttcagacc ataccgaggg gcacgggtga gtgggcgacc accagccttt
      241 cgcctcgtgc cacgagccgt tcgaatgagg tatgatctcg ccgcttcagt gtacgcaagg
      301 gcgtcactga ttttcaaacc tgggacgaga tcgatgaaat gaaagaattg gtgccagctg
      361 aaatcgagat gtacgcgcaa gcgcattcga tgccggagtc ggatctctgc cgggagttgc
      421 gcgcagaaac aaccagacgc atggagtttc cccagatggt cgtcggaccg ctggaaggcg
      481 cgttcttgaa gatgatgacg cagatcgtcg gcgcgaaacg agttctcgaa atcggcatgt
      541 tcacgggcta tagtgcgctc tgtttcgccg aagcgttgcc gaaggacggg accatcctca
      601 cctgtgaagt cgacgatgag tcagccgccc ttgcgcgcgc ctactttgcg cgatcgccgc
      661 atgggaggaa aatcgaaatt cggatgggac cagccttgga cacgatgcga tccctcactg
      721 gtccgttcga tctgatcttt atcgatgcag acaagacgaa ctacgtgaat tactacagac
      781 gggcacttga tctcatttca ccgaatggag tcatcctcgt cgacaacgtg ctgtgggatg
      841 gtgaagtatt actccagccg ccaccggacg agaggaccgc agccattcag gagctcaacc
      901 gagttgtggc tgccgattcc cgtgttacgg ctgtgctgct tacgattcgg gacggtgtgt
      961 tgatgattcg accgaacgtg aatgtctcac gatgagccaa cccacggaca tgggagccta
     1021 cggtgcgctc gtcactgcgg ccgtgttgtg gggcggctcg atcgtggcgc agaagttggc
     1081 gctggggtcc ttctcagccg tcgaggcctc ggtccttcgc gacattgggg gcttgatcat
     1141 tctccttgca acctggtggt ggcaggacgg cacgtttgcc aaattgacga aggcggatgc
     1201 gcagacactc tgctggctgg ggttgtgcgt tcttgggaat cacctcctga ttctcatcgg
     1261 ccttcggtat gtgagcggcg ccgtagccgg ggtcatcatc ggatcgagcc cggtggtgac
     1321 gtcgttattg tctgccatct tgattcaaga cgttcccctg cgcaccgtgt ggatgggcgc
     1381 tgttctgtcc tgcgcgggag tcgggttggt gtcagtggcg ggattcagtg cagcgggcga
     1441 tcagccactg ctgggcgggt cgttggtgtt tctcggtgtg gtgagttggg cgctgtacag
     1501 catcggcagc cgctcgatca tggaacgggt cccggcgctc acagtcaact ggacgacgct
     1561 actcgtcgcc acggctctgc aacttccgct gctctggacc gaccggaaga tgctggaggc
     1621 gggcccggcg tcggtggcga ctccggattg gttcgccctc ggctacctcg tggtgtttgc
     1681 cactgccgtc gcgcagcagg cgtggttgtt cggggtcaag ggcgtcggtc catcacgcgc
     1741 ctcggtgttg ggcaatctga cgcccgtggc cgcggtcggg ctctcggcgc tgatcctgca
     1801 cgaatcggtt ggtgcggtcg aagtcatcgg catcgtgctg attctggccg gagtctgggt
     1861 ggtccaccag cagacgtcta gactctcgcc atcataagcc cgccttttct ctgctcgatc
     1921 tgctctgccg attccagtat gatacggctc ctctttgtgg gcaggttgtt caaaaagatc
     1981 atccagcaag gccgcagcaa gcgaggaggt gaggcgtacg tcgttctgta cgttgaacct
     2041 ctgagtgatg cgagaacgaa gctgatggtc tttttcaaca tcctgctctt ggagcccgca
     2101 tgatcgagcg gaggatcgaa gaagtcacca aagccaacca gcggttttac gaagcctttg
     2161 aaagcctgga cattgccaag atggatgaag tctgggcgca tctcgagtac gtcacctgcg
     2221 tgcatcccgg ttggaccctc cgctccgact ggcctgccgt gcgcgattct tgggtactga
     2281 tcttcaacaa taccttctcg atgaaattcg agctgaccga agttatggtg caggtggcgg
     2341 gcgatatggc ctgggtcatc tgcgtcgaaa acatcaccag ccagcaatcc gatgagcccc
     2401 agcaggcgaa ggtcctggcg acgaatctct tcgagcggat cggggatgag tggttgatga
     2461 ttcatcatca tggaagtgcg gtcatgggct gatggttccc ctgtgctccc ggaacgcgca
     2521 caataatact gtgctcgttc gacggccgca gttggggaac cattcagccc atgaccttgg
     2581 gagagtaggg ggatggggaa gctggtgctc gttcaatgcg cgcagtcgaa acacaaccca
     2641 ggccctagcc ccttgaagag gtaaggggga aaagagtgtt gctcgttgga cgcgcgcaat
     2701 cggaggccac cccgttacct caccactagg gagtggaggg gtaagaaaaa agacttatcg
     2761 acgcgcgtgc gttgagagct gacaggcccc ccattgagaa tttggcaaga gaaaagaact
     2821 gtgctcgtcc gacgggcgta gtgggggaac caatcagcct gtgaccttgg gagagtgggg
     2881 gagagagcgg tgttcgatca atgcaagcac gttgtaggac aggcgtttgt taatcacggt
     2941 agaggccaag aatgagaatt gtctcgctgt tgccgagtgc gacggagatg attt
//
LOCUS       DATDNW010000322         9590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_254506, whole genome shotgun sequence.
ACCESSION   DATDNW010000322 DATDNW010000000
VERSION     DATDNW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254506"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..907)
                     /gene="purU"
                     /locus_tag="VI359_07685"
     CDS             complement(44..907)
                     /gene="purU"
                     /locus_tag="VI359_07685"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEY6974171.1"
                     /translation="MSRRRDSVVLLIHCKDRKGIVARVSGFIHDFGGNILDSDHHTDE
                     ETNDFLMRMEFATEGLQIPPDDIPVAFAPIAKVFEMRFEVHRTGHRTRVGMLVSKQDH
                     CLADLLQRQKRDELHVDIPVIISNHETCASWAELFNIPFVVCPVTKETKAQQEQQLLS
                     VLKKHRVELVVMARYMQILSATFLAQVGCPVINIHHSFLPAFIGANPYRQAYERGVKI
                     VGATAHYATEDLDEGPIIEQDVIRVGHRDTVDDLVSKGRDLEEIVLARAVRRHIEHRV
                     LVYGRKTVVFD"
     gene            complement(989..2428)
                     /gene="rimO"
                     /locus_tag="VI359_07690"
     CDS             complement(989..2428)
                     /gene="rimO"
                     /locus_tag="VI359_07690"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY6974172.1"
                     /translation="MTSSLIQLGRKGPGRHAAGSTTIGFVNLGCSKNQVDSEIMLGTL
                     VQDGFELTGDPKKAEVVIINTCGFIEEAKQESINSIIEHGKLKRSGACRVLIAAGCLA
                     QRYQSDLIKELPELDGVVGTGEFGKIAEICRALLAPTKRQQRLWISEPPYLYDAEAPR
                     LRLGKVHSAYVKIAEGCNRNCAFCAIPIMRGKQRSRSVESIVAEAKRLAAEGVKEINL
                     ISQDTINYGVDLGQRQGLVTLLRELVKVEGLRWIRPFYLYPQQVTDELLDLYAGEEKI
                     TKYIDMPLQHINDRMLKRMHRLGDRGAITSLVERIRTRIPGVAFRTAFIVGFPGETEQ
                     AFEELKSYIQETEFDRVAVFLYSDEEDTPAVSLDSKVDRSVMEERRNEILAVQESIAA
                     AKGRARIGSVVEVLIEGVSEETEWLLEGRHEGLAPEIDGVVYINEVVDNHTHSSRARQ
                     GASVPVPGQMVKIEVTDAALYDLVGHIVE"
     gene            2514..3053
                     /locus_tag="VI359_07695"
     CDS             2514..3053
                     /locus_tag="VI359_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY6974173.1"
                     /translation="MLRTFQGRKPTVPKSCFIEETGVVIGDVVMGEDCSVWFHAVIRG
                     DVHFIRIGDRTNVQDLCVLHVTHDTHPLIIGNDVTIGHNVILHGCTIRDRVLIGMGAI
                     IMDGAVIGEDSVVGAGALVTEGTVVPAKSLILGSPAKVKRAVTENELSWIKESADNYV
                     NYARQYLGGPEKTRTGFQF"
     gene            complement(3084..3776)
                     /locus_tag="VI359_07700"
     CDS             complement(3084..3776)
                     /locus_tag="VI359_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974174.1"
                     /translation="MSRSLNEATRRTIARVYERLDYRALGHVYCYEGGDEFWRAKRRP
                     CQRLGSLVAAALRARLARGGRSLYVGAGVAELPVLLAETLELQRTVEPYNLRRSEVAI
                     LNRACRPSSVTFRAQDATTARGRFDHLWIVSVLNDPERFPHLSRLSYGRADPVTFDSR
                     RFEKERRKVRRIIDRCMPKLTRPGLVTTSTEEVVWIAEWCHRHGIPYLVERRQYATAL
                     VGDPICFIKIRK"
     gene            complement(3788..4657)
                     /locus_tag="VI359_07705"
     CDS             complement(3788..4657)
                     /locus_tag="VI359_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY6974175.1"
                     /translation="MSPAHFRIRRHLQSLVGERHPERSPRALKKTAHYLATQFAKSGW
                     KVSHQSFNALGDTYRNVVAVKAPSSFIGGAYPSPLLIGAHYDTVPDSPGADDNASGLA
                     VLLETAARLQSVALSRPVWLIGFCLEEQDRLGSRVFAARLKAESQALAGVIILECVGF
                     SSNRPASQQAPPGVPQAVPTVGDFLAIVGNESARNLVQAIERSAATHAPALKVQSFVV
                     PGCGEAVPHTRRSDHASFWDANYPAVMLTDTANFRNPHYHQETDTIETLDVRFLVGVI
                     DTVTAATVELAGA"
     gene            complement(4684..5583)
                     /gene="argB"
                     /locus_tag="VI359_07710"
     CDS             complement(4684..5583)
                     /gene="argB"
                     /locus_tag="VI359_07710"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY6974176.1"
                     /translation="MNKLIKKANVLIEALPYIRTFKGKTIVIKYGGHAMTDVSLKERF
                     AQDVVLLKYVGLNPVIVHGGGPQIDKMLDRLGIEAKFRHGVRVTDEATMEIVEMVLAG
                     KINMEIVDLLNRHGGRAVGLSGKDGGLMLTRPLTAKAWAESLEKDLDDDEEEGEDFGF
                     VGEVQSVDPSLVLKLQQDHYIPVIAPIGTDREGSTYNINADLVAGAIAGALGAEKLVM
                     MTDVKGIRDAKGRHLSTVSRKDVQRMVKKGTISEGMLPKVHACLDALAGGVGKAHIID
                     GRISHAILLEVFTHKGIGTEIVA"
     gene            complement(5825..6772)
                     /gene="xerC"
                     /locus_tag="VI359_07715"
     CDS             complement(5825..6772)
                     /gene="xerC"
                     /locus_tag="VI359_07715"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY6974177.1"
                     /translation="MDKAVRAFLTFLEIERGASRETLRNYASDIRQFVSFLIAGVAES
                     RPIDPAAVQSDSVRAYLQWLDRKHEKRTSLARKLACLRSFFRYLQREGVVSQNPAEDV
                     RSPKLPKQLPRVLTKDEAQALLELPDATTGLSLRDRAILETLYSTGARVSEIVGINVE
                     DLSTKEGLVRLQGKGRKERIVPIGDVAVEAIRHYRVWLDSRLNGQHSRPDKMALFRNS
                     RGGRLTTRSVARLVARYSRVLPGGPVSPHTLRHSFATHLLDEGADLRAIQEMLGHASL
                     STTQKYTHVAMDQLLALYDKTHPRATRSMKSGPTKGGKR"
     gene            complement(6745..8061)
                     /gene="trmFO"
                     /locus_tag="VI359_07720"
     CDS             complement(6745..8061)
                     /gene="trmFO"
                     /locus_tag="VI359_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY6974178.1"
                     /translation="MRDDIVIIGGGLAGSEAAWQAANRGAKVTLYEMRPKEMTPAHKT
                     GGLAELVCSNSLGSIDLQNAPGILKEEMRRLNSLIIRAADQSRVPAGSALAVDRERFS
                     FTITQALESHPNIRILREEVPDIPTDCLCVVATGPLTSDKLSQSIAQLTHTKHLYFYD
                     AISPIIDTDSIDMDVVFRASRYDKGGDDYLNCPMDEATYNTFYEALLAAEKVQPKEFE
                     KTPYFEGCIPIEVMAERGRQTMQFGPLKPVGLVNPKTGTRPFAVVQLRTENAHCSCYN
                     LVGFQTKLTYGEQKRVFRMIPGLGRAEFLRYGSLHRNTFINSPQLLRDTLQFKARGTL
                     FFAGQLVGVEGYTDSAAMGGLAGINAARGLAGLPLVTPPSTTAHGCLVSHITAADPRF
                     FQPMNINYGLFPPLVSPVRDKEQKRRLIGQRALEDLTTWIRQFGLS"
     gene            8267..8341
                     /locus_tag="VI359_07725"
     tRNA            8267..8341
                     /locus_tag="VI359_07725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8299..8301,aa:Val,seq:cac)
     gene            8522..8866
                     /locus_tag="VI359_07730"
     CDS             8522..8866
                     /locus_tag="VI359_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974179.1"
                     /translation="MRTTALAVGMLLLVIVTGCASNRQEPTTADLGDAEYRAPASIYS
                     VMDSTALVYTNPVAGSPINDNPWRWTGFILHPVGMALDYGFNRPLYKLTSSMPYLFGY
                     TSEDAMLDSQRQ"
     gene            9182..>9590
                     /locus_tag="VI359_07735"
     CDS             9182..>9590
                     /locus_tag="VI359_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974180.1"
                     /translation="MASLKQKAWGTGHELILPSDRSPSRADESYFFDEDGFLVGALFS
                     FPSGLDLSPYPVLRDTLAKLKPVLEFYVNVANLSSKDSMNSSTLYETGDEKTTTQYLV
                     LAAGEHPTLLQASITIDPYARLFSPYRREFLERL"
BASE COUNT         2085 a         2781 c         2570 g         2154 t
ORIGIN      
        1 cgagccctgc tccctcttga tctgccccgt tcaatacctc ccgctagtcg aagacgactg
       61 ttttcctccc atacaccaac actcgatgct cgatgtgccg gcgcacagcc ctggccagca
      121 cgatctcctc gagatcacgt cccttgctca cgagatcatc aaccgtgtct cggtggccga
      181 cccggataac atcctgctcg ataatgggac cttcatccag atcctccgtc gcatagtggg
      241 ctgtcgcccc tacaattttg acgcctcgtt cataggcctg ccgataggga ttggcgccga
      301 taaaagcggg caagaatgaa tggtgaatgt taatgacagg gcaacccacc tgagcgagaa
      361 aggtggcact gaggatctgc atgtagcgag ccatcaccac aagctcgaca cgatgtttct
      421 ttaagacaga gagtagctgt tgctcctgtt gtgctttcgt ctctttcgtg accggacaga
      481 ccacgaaggg gatgttgaac agttcggccc acgaagcaca ggtctcgtga ttggagataa
      541 tgacggggat gtcgacatgc aactcgtctc gcttctgccg ttgaagcaga tcggccaagc
      601 agtggtcctg tttcgagacg agcattccga ctctcgtacg atgaccggta cgatggactt
      661 cgaatcgcat ctcgaagacc ttggcgatgg gggcgaatgc gaccggaata tcatcaggtg
      721 gaatctgcaa cccctcagtg gcaaactcca tccgcatcag aaaatcgttg gtctcttcat
      781 ccgtgtgatg atccgaatcg agaatattgc ccccgaagtc gtgaataaac ccggaaacgc
      841 gggccacgat acctttacga tccttgcaat ggatcagaag gacgacggaa tctcttctcc
      901 tcgacatgaa ccctcgatga cttcccgaat gtaatgcccc gttatgtagg agatgaagcg
      961 gcgggcggtg agaaaatgaa gaccaccatt attcgacgat gtggcctacg agatcgtaga
     1021 gggcggcatc ggtgacttcg attttgacca tctgacctgg cacagggaca gatgcgcctt
     1081 gacgcgcccg ggacgaatgg gtgtgattgt cgaccacttc gttgatatac acgaccccgt
     1141 cgatttctgg agccaatcct tcatggcgcc cctccagcaa ccactcggtt tcttcggaaa
     1201 ctccttcgat caacacttcc acgacggagc cgatgcgcgc acgccccttc gcagcggcaa
     1261 tgctttcttg cacggccagg atttcattcc ggcgttcttc catcaccgac cggtcaactt
     1321 tgctgtcgag tgagacagcc ggtgtatcct cctcatccga atacagaaag accgcgactc
     1381 gatcgaattc tgtctcttgg atgtaggact tcagttcctc aaacgcctgc tctgtttccc
     1441 ccggaaatcc cacaatgaag gcggtgcgaa acgccacacc aggaatgcgt gtgcgaatac
     1501 gctcgacaag cgaggtaatg gctccgcggt ctcccaatcg gtgcatccgc ttcagcatgc
     1561 gatcgttgat atgttgaagc ggcatatcga tatatttcgt gattttttcc tcacccgcat
     1621 agagatcaag caattcgtcc gtgacctgct gcgggtagag atagaacggc ctgatccacc
     1681 ggagaccctc cactttgacc agctctcgca agagcgtgac gagtccttgc cgttgaccga
     1741 ggtctactcc atagttgatc gtgtcttggg agatcaggtt gatctctttc accccttccg
     1801 cagccagtcg cttggcctct gccacaatcg attccaccga ccggctgcgc tgcttgcccc
     1861 gcatgatcgg gatcgcacag aatgcgcagt tccgattaca cccttcggcg atcttcacat
     1921 aggcactatg aaccttcccc agccgcagac gaggggcctc cgcgtcgtac agatacggag
     1981 gctcactgat ccagagacgt tgctgccgtt tggtaggcgc aaggagggct ctgcaaatct
     2041 ccgcaatctt cccaaactca ccggtcccaa cgacgccatc aagttccggc aattccttga
     2101 tcagatccga ctggtagcgt tgcgccaaac agccggcagc aattaggact cggcaggccc
     2161 ccgaccgctt gagcttgcca tgctcgatga tcgaattgat cgattcctgt ttggcctctt
     2221 cgatgaaacc acaggtattg ataatgacga cctcggcctt cttgggatca ccggtcagtt
     2281 cgaatccgtc ttgaacgagg gtgcccagca tgatttcgga atcgacctgg ttcttggaac
     2341 aacccagatt cacaaagcca atggtagtcg agccggcggc atgccggccc ggtccttttc
     2401 gaccgagctg aataagcgaa gaggtcatac ggcaccagtc tacgggagcg tgaaaaagcc
     2461 tgtcaattga gaggccttgc gcgtagaagt cagattcccc tacagtgcgg cctatgctcc
     2521 gcacttttca aggcagaaaa cccaccgttc cgaagtcctg tttcattgaa gaaaccggtg
     2581 tggtaatcgg cgatgtggtg atgggagaag actgcagcgt gtggttccac gccgtcattc
     2641 gaggggatgt acacttcatc cgaatcggag accgcaccaa cgtgcaggat ctctgcgtat
     2701 tgcacgtcac ccacgacaca caccctctga tcatcgggaa cgacgtgacg atcggccaca
     2761 acgtgatcct ccacggctgt acgatcaggg atcgggtgct gattggaatg ggcgccatca
     2821 tcatggacgg agccgtgatt ggagaagact cggtcgtggg tgctggagca ctcgtgactg
     2881 aagggacggt cgtcccggcg aagagtctga tcctcggttc acccgccaag gtaaaacgag
     2941 ccgtcaccga aaacgaattg tcgtggatca aggaatcagc ggataactac gtcaactatg
     3001 cgcgtcagta tctaggtggt ccagaaaaga caagaaccgg ctttcagttc tagctttcct
     3061 gatcttttgc ctcgatcgtt atttcacttc ctaatcttga tgaaacaaat cggatcgccg
     3121 acgagcgctg tcgcatactg ccggcgctcg acgagataag gaatcccgtg acgatgacac
     3181 cactcggcga tccagaccac ttcttccgta gaggtcgtga caagccccgg ccttgtgagc
     3241 ttcggcatac agcgatcgat gatcctgcga accttcctgc gttccttctc gaatctgcgc
     3301 gagtcgaagg tcacaggatc ggcccgcccg tatgagagcc gtgacagatg gggaaagcgt
     3361 tcaggatcat tgagcacgct cacgatccag agatgatcga acctgccgcg agctgttgtc
     3421 gcatcctgcg cccgaaatgt cacggatgac ggtcggcagg cacgattgag tatcgcgacc
     3481 tcagatcggc ggagattata cggctcgacc gtccgctgaa gctcgagcgt ttcagccaac
     3541 agaaccggca gctctgcgac gcctgccccg acatacaggc ttcggccgcc gcgagcaagc
     3601 cgggcccgca atgccgcggc cacgagactc cctaaacgct gacagggccg gcgctttgcg
     3661 cgccagaatt cgtctccccc ctcataacaa tacacatggc cgagggcgcg atagtcgagc
     3721 cgctcatata cccgagcgat tgtccgccgg gtggcttcgt tcagagatcg cgacacggat
     3781 cttctcatca tgctccggcc aattccactg tcgcagcagt cacagtatca atcaccccga
     3841 cgagaaatcg cacatccagc gtctcgatcg tatcggtttc ttgatggtag tggggattgc
     3901 ggaagttcgc cgtgtcggtc agcattacgg ccgggtagtt ggcatcccag aacgaggcat
     3961 ggtcacttcg cctcgtatgg ggtacagctt caccgcatcc cggtacgacg aaggactgta
     4021 ctttcagtgc aggagcgtga gtcgcggcac tccgctcgat ggcctgcacg agattccgcg
     4081 ccgactcgtt tcccacgatc gccaaaaaat ctccgacggt cggaacagcc tgcggcacac
     4141 caggcggagc ctgctgtgat gccggacggt tcgaagagaa tcccacgcat tccaggatga
     4201 tcacgcccgc caacgcttga ctctcagctt tcagtcgagc cgcaaagacc cgactgccca
     4261 aacgatcctg ttcttccaaa cagaagccga tcagccacac cggacgggac aatgcaacac
     4321 tttgcagccg cgcggccgtt tcgagcagca ccgcgagtcc gctcgcatta tcgtcagccc
     4381 ctggtgagtc aggtacggta tcgtagtgtg ccccgattag gagcggagac ggataggctc
     4441 cgccgataaa agaagatggg gccttgacgg ccacgacatt gcgataggtg tcgcctaatg
     4501 cgttgaacga ttggtgagag accttccatc ccgattttgc aaactgcgta gcgagatagt
     4561 gcgcggtctt cttgagcgcg cgtggactcc gctcgggatg gcgctcgccg acgagagatt
     4621 ggagatgccg tctaattcgg aaatgtgccg gtgacacaag aagggccaga cggagctgca
     4681 gcgttaggcg acgatttcgg tcccgattcc tttgtgcgta aacacctcaa gaagaatcgc
     4741 gtgtgagatg cgcccatcga taatatgggc tttgccgacc ccgccagcca acgcgtccag
     4801 acaggcatgg accttgggca acatcccttc gctgatcgtc cccttcttga ccatccgttg
     4861 aacgtccttt cgtgaaacag tcgacagatg gcggcccttg gcgtcacgga tacctttcac
     4921 gtccgtcatc atgaccagtt tttcggcgcc caaggcccct gcgatcgcac ccgcaacgag
     4981 atcagcgtta atgttgtagg tgctgccttc ccggtcggtg ccgatcggag cgatgacggg
     5041 gatgtaatgg tcttgctgga gcttcagcac caagctcggg tccacggatt ggacttcgcc
     5101 gacaaacccg aaatcctctc cttcctcttc atcgtcatcc agatcttttt cgaggctttc
     5161 ggcccaggcc ttggcggtga gcgggcgtgt cagcatcagg ccgccgtctt tcccgctcaa
     5221 tcccacggcc cgaccaccat ggcgattcaa gagatccacg atttccatgt tgatcttccc
     5281 agccaagacc atttccacaa tttccatcgt ggcttcatct gtcaccctga caccatgtcg
     5341 gaacttggct tcgatgccca accgatccag cattttgtcg atttgtggac cgccgccatg
     5401 aacgattacc ggattcaggc cgacatactt gagcaatacg acgtcttgtg cgaaccgctc
     5461 cttgagcgac acgtccgtca tcgcgtggcc gccgtacttg atgacgatcg ttttgccctt
     5521 gaaggttcgg atgtagggca atgcctcaat gaggacgttt gccttcttga tcagcttgtt
     5581 catcgttcgc ccccaactct cagctcgtaa ccatcagcta cccgcttttc atctcaaacg
     5641 cggttgatgg ttgggagctg gccgtcgata gctcgttgca ggatctagcg ggtcacatct
     5701 tggccgcgcc gattctatca atttcaccgg gaaatacaat cccagtcact tgaattttcg
     5761 cgacagagct gtggacgtct ttgcagccga gatgggcttt tgctcatcag ggatcgaaat
     5821 gtcgtcatct cttgccgccc ttggtcggac cacttttcat gctacgggtg gctcgcgggt
     5881 gagtcttgtc gtacaaggca aggagttggt ccatggcaac atgcgtatac ttctgcgtgg
     5941 tgctgagcga ggcgtgtccc agcatttcct ggatcgctcg caaatcggct ccctcatcga
     6001 gaagatgcgt agcaaacgaa tgccgcaacg tatgagggct caccgggccg ccggggagca
     6061 cacgcgagta ccgcgccaca agtcgggcaa cactgcgcgt cgtcagtctc cctccgcggc
     6121 tgttgcggaa gagcgccatc ttgtcggggc gtgagtgctg accgttgagg cgtgagtcga
     6181 gccaaaccct ataatgtcgt atggcttcca ctgccacgtc gccgatcggc acaatgcgct
     6241 cttttctgcc ttttccttgc agacggacaa gtccctcctt tgtactgagg tcctcaacat
     6301 tgatccctac tatttcgctg acacgggcgc cagtcgagta gagggtttcg agaatggcgc
     6361 gatcgcgcag tgacaaaccg gtcgttgcat ctggcaattc cagcaaagcc tgggcctcgt
     6421 cttttgttaa gacacgaggg agctgtttgg gtagcttggg ggatctgaca tcctcggcag
     6481 gattttgtga aacaacccct tctctctgaa ggtaccgaaa aaagctacgg agacaggcga
     6541 gttttcttgc taaggaggtg cgtttctcgt gcttgcgatc cagccactga agataggcgc
     6601 ggacactatc ggattggaca gcagcaggat caataggccg actttcggcg acgccggcga
     6661 tcaagaatga aacaaattgc cggatgtcag acgcataatt tcgcagcgtt tctcttgatg
     6721 ctcctcgctc gatttcgaga aacgtcagga aagcccgaac tgccttatcc atgtggtcag
     6781 gtcctcaagg gcgcgttgcc cgatcaatcg ccgcttttgc tccttgtcgc gaacaggact
     6841 cacgagggga gggaagagcc catagttgat attcatcggc tggaaaaaac ggggatccgc
     6901 cgccgtgatg tgggacacga gacaaccatg cgcggtggtt gaaggcggcg tcaccagagg
     6961 aagtcccgcg agcccacgcg ccgcattgat accagcgagc cctcccatcg ccgcagagtc
     7021 cgtgtatccc tccacgccaa ccagctgccc cgcgaagaac agggttcctc gtgccttgaa
     7081 ctggagcgtg tcgcgcagca attgcggcga attgataaag gtgttgcgat gcaggctgcc
     7141 atagcgaagg aactcggcac gtccgaggcc ggggatcatg cggaacaccc gtttctgttc
     7201 cccgtaagtc aatttagtct ggaatccgac caggttgtaa caggaacagt gcgcgttttc
     7261 cgtccgtagc tgtacgacgg caaacggcct cgtgcctgtt ttcggattga ccaagcccac
     7321 cggtttcaac ggaccgaatt gcatcgtctg tcgtcctcgc tcggccatca cttcaatcgg
     7381 aatacagccc tcgaagtagg gcgtcttttc gaattctttg ggctggacct tctccgccgc
     7441 caacaaggct tcataaaacg tgttgtatgt cgcctcatcc atcgggcagt tgagataatc
     7501 gtcaccgccc ttgtcgtaac gtgaggcgcg gaagacgacg tccatgtcaa tggaatcggt
     7561 atcgatgatc ggagaaatgg catcgtaaaa atacaaatgt ttcgtatggg tcagctgggc
     7621 gatcgattga gataatttgt cggacgtcaa aggaccagtg gctaccacac agaggcagtc
     7681 cgtcgggata tcgggaacct cttccctcag aatgcgaata ttagggtgac tctccagggc
     7741 ttgggtgatc gtgaaggaaa accgctctcg atcaacggcc aaggccgaac cggctggaac
     7801 gcgggattga tcagctgcgc ggataatcag cgagttgaga cggcgcattt cctccttcaa
     7861 aatgcccggg gcattttgaa ggtcgatgga gccgagcgaa ttggaacaga cgagttctgc
     7921 taagccaccg gttttatgag ccggcgtcat ttccttcggc cgcatttcgt agagcgtgac
     7981 tttggctcct cgattggcgg cttgccaagc tgcttctgac ccggcaagcc ctccaccgat
     8041 gatgacgatg tcgtctcgca ctgcgcgaca ctccatgagg aagaaactgc gggagacggc
     8101 attctaggaa ccgtgttttc gcgaagtcaa ggtaagctga tcgcagtatt gaggtgcaag
     8161 gcatggggcc ggcgttctat cttcaaatcc ccagtcgcaa accccttgcc tccggctgat
     8221 gttcgattga tctgctcaag aggcacgtgc taaactccgc caccgtgggc gattagctca
     8281 gtgggagagc gcttccttca cacggaagag gccactggtt cgataccagt atcgcccacc
     8341 atttcggcag gggtatccct ccattcttct cctaaacgcg caaagattcg agttttttaa
     8401 ggggttggtt gaaaccgctt gttttctaga cagaattcgg aacgtcggct acacttcact
     8461 ttgtcaaatc agtatcaatc agaatttagg tcgaaccgac gtagacaaag tgaggttagc
     8521 catgcggaca actgctcttg ccgtgggtat gcttctcctg gtgattgtta ccgggtgtgc
     8581 aagcaatcgc caagaaccga ccacggcaga cctcggagat gcagagtatc gggccccggc
     8641 cagtatctac agcgtcatgg acagcaccgc cttggtgtat accaacccgg tcgctgggtc
     8701 accaatcaac gataacccct ggcgctggac cggattcatc ttacaccctg ttgggatggc
     8761 attggattac ggctttaatc ggccactata caagctcacc agctcgatgc cctatctctt
     8821 cggctatacc agtgaggacg cgatgctgga cagccagcgc caataacctt agtttcctct
     8881 cgtgcatctg acccaacgcc cactcagagt ttgagtgggc gttgtcattt ctcctcttgg
     8941 caaaccagtt cccatctcgt gtatcctccc ctttgtgaac ttgagcatcc aaattgtgga
     9001 accacgtctg ccttttctgc accatctcgc gctaattttt ggggtaggac tatcccttct
     9061 gctcccaggt tgtgaaaccc ctgttccgcg gcccccactg ccaccgtccg aaagcgattt
     9121 agtcctgttg aagtcggccg cggtctgcag tcgaagagcc gaatttaata aaaaccaccc
     9181 catggcgtcg ctcaaacaaa aggcgtgggg gactggacat gaattgatcc ttccatccga
     9241 ccggagtccg tcacgcgccg acgagtcgta tttcttcgat gaagacgggt tcttggtcgg
     9301 cgcactcttc tcattcccct ccggcctgga tctttctccc tatcccgtgc tccgcgatac
     9361 gcttgccaag ctaaagcccg tgctcgagtt ttacgtcaat gtcgcgaatc tctcatcaaa
     9421 ggacagcatg aactccagta cgctctatga aacgggagac gagaaaacga ccacccagta
     9481 tttagtactc gctgcagggg aacatccgac gctactccaa gcctctatca caatcgatcc
     9541 ctacgcgcgc ctcttctctc cttaccggcg tgaatttctt gaacggttgc
//
LOCUS       DATDNW010000323         7662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_591746, whole genome shotgun sequence.
ACCESSION   DATDNW010000323 DATDNW010000000
VERSION     DATDNW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591746"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VI359_07740"
     CDS             complement(<1..1347)
                     /locus_tag="VI359_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY6974181.1"
                     /translation="MEQYPRRRFWLLGPLVAIAIVFGVLIGKGWERTGHATETYEELK
                     TFSEVLTQVQKNYVDETKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVIAPIEGTPAHRAGIKAGDFITKVNDESTKDLTLMDAVQKMRGP
                     KGTKVNLTVQRDGTPDLLQFTLVRDTIKIESVKAKTLENMGYVRLTQFQEATGRDLSK
                     ALKQFREQKVQGTILDLRNNPGGLLTAAVEVSEQFLPSGKLVVYTKGRESKKDEWFAK
                     GKDQMEDAPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDGS
                     GLRLTTAKYYTPKGRSIQATGITPDIVVKIQPTTVAKAGEGKAGEKEADAKTAKPPAA
                     KDQPATNGKPSEEGAKNGTTPTPPPFDVAGEPSLEQDVQLQKAVELLKSWKIFKGLP"
     gene            complement(1372..2556)
                     /locus_tag="VI359_07745"
     CDS             complement(1372..2556)
                     /locus_tag="VI359_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY6974182.1"
                     /translation="MTGSKHLLLAAVIVGNLYPTVSIEAGTDALSDKIEKERKNLDQL
                     KDQIEEKRKQADEAGKKRDSVLQGLQSLDERLVQYRQAHQDISRKLRKKDQEIEMLTR
                     QMSSLQDRIDERRDAILARLRIQYMEGRLGHWKTLLASDSYGDFQRRLQYLSAVSERD
                     YALMGTFRADVEQMQHAERQREEARTGMLAYKQSTEKHLSNIKSLKKEKRVYLTTITH
                     EKDSYERALQELERSASRVDSLLRELENRRRAAMAKPPAGSLPRGLKGLLPWPAEGNV
                     VTYFGRQKHPTFNTYVQRKGIEIRTPEGSAIHAVMPGAVVYADWLKGYGLVIIIDHAN
                     GFFSLYAHASKILTTVGAQVTAGQAIGETGDTGMTGENTLYFELREGADPVDPLQWLA
                     KR"
     gene            complement(2553..3458)
                     /gene="ftsX"
                     /locus_tag="VI359_07750"
     CDS             complement(2553..3458)
                     /gene="ftsX"
                     /locus_tag="VI359_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY6974183.1"
                     /translation="MGMRTVFYLLREAWANLRTNRTTTMVAILTTAFTLACVGIFLLL
                     YVNLRAAAGWLQEDVKIMVYLDDRVSRESAAAIEQQLRSDHALASLMFISKEQALGEF
                     KAQFPSESHLLEGLGQNPLPASFVVTLSPQYRAPDAVRRWAERVRTITGIAKVDYNQD
                     WINALATFIRSIELIAIGVGVVLSVAAVTIIGNTIRLTLYARRDELAILRLIGATTTF
                     IRVPYLLEGAALGALGSAVSLIMLKIVYEVFRQQVRAMGRLAGLEQMVTFFSPQICLA
                     LIVVGMGLGCAGSFVSLRRFGEAKV"
     gene            complement(3449..4117)
                     /gene="ftsE"
                     /locus_tag="VI359_07755"
     CDS             complement(3449..4117)
                     /gene="ftsE"
                     /locus_tag="VI359_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY6974184.1"
                     /translation="MIQLIHVSKYYNRRAALADVTLDIEKGEFVLLMGPSGSGKSTLL
                     RMLIGAEQPDDGHVFVQGKNVTKLKSSEIPYLRRKIGTVFQDFRLLPKKSVFDNVALP
                     LLVQGASPVDIRRKVTEALRAVGVDHKRDQLPIGLSTGEQQRVCVARAIVNGPVVLLA
                     DEPTGNLDPGLTSEIMELFKLINARGTTVVVATHDPLVMAQVNRRVVKLDQGMLVPEQ
                     RVWA"
     gene            complement(4125..4511)
                     /locus_tag="VI359_07760"
     CDS             complement(4125..4511)
                     /locus_tag="VI359_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY6974185.1"
                     /translation="MLDPRRLFGQEGESVAERYLRRKGYRVLERNARSSVGELDLVME
                     DGAVLVFVEVKSRHSDAYGGAVHAVHRRKQDKLIQLAAQYLARHHITNRPCRFDVVLV
                     QGTEANDAQVDHIQNAFEVSGDDLRW"
     gene            complement(4566..5186)
                     /locus_tag="VI359_07765"
     CDS             complement(4566..5186)
                     /locus_tag="VI359_07765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY6974186.1"
                     /translation="MEPTDDFEVEARRCGYRRIAGLDEAGRGPLAGPVVAAAVILPAR
                     CCLTGINDSKQLSESEREGLYDMIVRRAIGVGVGIATEQEIDTLNILEATRTAMHRAV
                     DALLVTPDCLLTDAMELPGVDIPLRAIIKGDALSRSIAAASIVAKVTRDRLMADYHRT
                     YPQYNFLSHKGYGTEEHLQRLAEYGPCSIHRRTFAPVAAVLAGRVS"
     gene            complement(5254..5685)
                     /gene="rplS"
                     /locus_tag="VI359_07770"
     CDS             complement(5254..5685)
                     /gene="rplS"
                     /locus_tag="VI359_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY6974187.1"
                     /translation="MNRLERIQRSLTKKSLSRFEIGDTVRVHVKVIEGEKERIQVYEG
                     VVIARKGTLNTEMFTVRKVSYGVGVERIFPVHSPIVTRIEVVRQGKVRRAKLYYLRGK
                     KGKFAKVEDREFVGESKGQPGAAQTEQLSEEAAEPAGAAKS"
     gene            complement(5682..6473)
                     /gene="trmD"
                     /locus_tag="VI359_07775"
     CDS             complement(5682..6473)
                     /gene="trmD"
                     /locus_tag="VI359_07775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY6974188.1"
                     /translation="MRCDVLTLFPDMIAPVFDQSMLKRAQEKGLLEVRVRNLRDYTLD
                     RHKVADDVPYGGGAGMVMKAEPILRAVETITAEYERAGEHTRLLFPSPQGRPFTQEYA
                     KNLAAEGRRLIILCGHYEGIDERVRLALQPEEISVGDYVLTGGELPALILIDAAARLV
                     PGVLGDPESIVEESFSDSTLEYPHYTRPSEVRGMTVPEVLLSGHHEAIRLWRRKEALR
                     NTYRRRPDLLRDRVLNAEDRQLLDELIREGVGSAPVRCGEGRITP"
     gene            complement(6463..6999)
                     /gene="rimM"
                     /locus_tag="VI359_07780"
     CDS             complement(6463..6999)
                     /gene="rimM"
                     /locus_tag="VI359_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY6974189.1"
                     /translation="MEATSADRVTIGKIERPFGVKGEVKVRSLSDVPGRFDHLEKVSV
                     VVSDGKAIEATVTHVRPAGPGFIMGLEGITSPEEAGAFRGGLIQVPRTIPSVAPEHTY
                     YECDLIGMTVESDLGAEIGTIESIWDVPGNYVLVVRKGSQEVLIPAARDLITKVDVAG
                     RRMTVRAIEGLIEYHDAV"
     gene            complement(7005..7289)
                     /gene="rpsP"
                     /locus_tag="VI359_07785"
     CDS             complement(7005..7289)
                     /gene="rpsP"
                     /locus_tag="VI359_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY6974190.1"
                     /translation="MAVHLRLTRTGRHKRPLYRVVAADSRKPRDGRFLEILGTFDPLK
                     EPAVPELKQDRVLTWLRQGAQPTVTVRTLLRRSGLWKQFEGEKAAKKKSA"
     gene            complement(7333..>7662)
                     /locus_tag="VI359_07790"
     CDS             complement(7333..>7662)
                     /locus_tag="VI359_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY6974191.1"
                     /translation="LGQVNRLGSLDQILGMLPGGQKLKGAMEGQLPEREMKRVAAMID
                     SMTSRERRDHTIINGSRKKRIARGSGTSVPEVNRLIKQFLGARKMAKAMAGTGGRRQL
                     AQLLRSM"
BASE COUNT         1440 a         2484 c         2024 g         1714 t
ORIGIN      
        1 agggagtccc ttgaaaatct tccagctctt caacagctcc actgccttct gaagctggac
       61 gtcctgctcc agtgacggct cgcccgccac atcaaacggt ggcggcgtcg gcgtcgtccc
      121 gttcttggct ccctcttctg acggtttccc gttcgtcgcc ggctggtctt tcgcggccgg
      181 cggcttcgcg gtcttcgcgt cagcttcttt ttcaccggct ttgccttcgc ccgccttcgc
      241 cacagtcgtt ggctgaatct tcacgacaat atccggcgtg atgcctgtcg cctggatcga
      301 ccggcccttc ggggtgtagt actttgccgt ggtcaggcgc aggcccgatc catcaccgag
      361 cgggagaatg gtctgcaccg aacctttccc aaacgacgtc gtcccgacaa tcaccgcccg
      421 tccccaatct tgcagcgccc cggccacgat ttcagaggca ctggccgaac cttcgttcac
      481 cagaatgatc atgggggcgt cttccatctg atccttgcct ttggcgaacc attcgtcctt
      541 cttactctct cgtcctttcg tatagaccac cagctttccg ctggggagga actgctcgga
      601 tacttcgacc gccgccgtca gcagcccgcc gggattgttc cgcaaatcca aaatcgtccc
      661 ttgaactttt tgctccctga actgtttgag cgctttgctc aggtcccggc cggtcgcttc
      721 ctgaaattgg gtcaaccgga cgtaccccat attctccagc gttttggctt tgacgctttc
      781 gatcttgatg gtatcgcgga cgagggtgaa ctggaggagg tccggggtac cgtcccgctg
      841 cacggtcaaa ttcaccttcg ttccctttgg cccccgcatc ttttgcaccg catccatcag
      901 cgtcagatcc ttggtggatt cgtcgttgac cttggtgatg aagtcaccgg ccttgatgcc
      961 ggcacggtgc gcgggagtcc cttcgatcgg tgcgatcacg gcgaggcgat tttctttcac
     1021 gccaatctga atccccaccc cgccgaactc acctttcgtc tccacctgca tctctttgta
     1081 catttcaggt gtcatgtagg ccgagtgcgg atcgagggtc gacaacatgc cgcgaatcgc
     1141 gccctgcacg aggtccttca ctttggtctc atcgacataa tttttctgaa cctgggtcag
     1201 gacctcggaa aaggtcttca gttcctcata cgtttccgtc gcatgccccg ttcgttccca
     1261 gcctttgccg atgaggaccc cgaacacgat cgcgattgcg accagcggtc ccagcagcca
     1321 gaaacgtcgt cgtggatact gttccatggt gctcacatcc tttcccgttc gttatcgttt
     1381 cgccagccac tggaggggat cgacggggtc cgctccctct cgcagttcga agtataaagt
     1441 attttcgccc gtcatgcccg tatctccggt ttcgccgatg gcctgaccgg ccgtcacctg
     1501 cgctccaact gtggtgagaa tcttcgaggc atgggcgtag agtgaaaaga atccgttggc
     1561 atgatcaata attataacga gtccgtatcc tttcaaccag tcggcataga cgacggcgcc
     1621 cggcatgacg gcgtggatgg cgctcccctc cggcgtccgg atttcaattc ccttccgctg
     1681 cacgtacgta ttgaaggtcg gatgtttctg ccggccgaaa tatgtgacaa cgtttccctc
     1741 agccggccag ggcaacaagc ctttcaaccc acgcgggagg ctcccggcgg gaggcttggc
     1801 catcgcagcg cgacggcgat tttccagttc gcgcagaagg ctgtccaccc gcgaggccga
     1861 acgctccagt tcctgcaggg cccgttcgta tgagtctttt tcatgggtga tagtggtcag
     1921 gtagacccgc ttttcctttt tgagcgattt gatgttgctc aggtgtttct ccgtgctttg
     1981 tttgtacgcc agcatcccgg tgcgggcctc ctcccgctgc cgctccgcat gttgcatctg
     2041 ctcgacatcg gcacggaagg ttcccatgag cgcgtaatcc cgctccgaca cggcggagag
     2101 gtattgaagc cgccgctgaa agtctccgta tgaatccgac gccaaaagtg ttttccaatg
     2161 gcccagccgg ccctccatat actggatgcg caagcgcgcc aagatcgcat cccggcgttc
     2221 atcgatgcgg tcctggagcg acgacatctg ccgggtcagc atttcgattt cctgatcctt
     2281 ctttcgaagt ttccggctga tatcctgatg cgcctgtcga tattgaacca gccgctcgtc
     2341 cagggattgg agcccctgca acaccgagtc tcgctttttc cccgcttcgt ccgcctgctt
     2401 gcgcttttcc tcaatctgat ccttcagttg atcgaggttc ttgcgttcct tctcgatctt
     2461 gtcgctcagc gcatcggtcc cggcttcgat cgacaccgtg ggatagagat ttcccacgat
     2521 tactgcggcg agcaagagat gcttcgatcc cgtcatacct tcgcctcccc gaaacgccgc
     2581 aaggagacga agctgccggc gcaaccgagc cccatcccga cgacgatcaa ggccaagcag
     2641 atctgcggag agaaaaatgt gaccatctgc tccaagccgg ccaggcggcc catcgcgcga
     2701 acctgctgcc gaaacacttc gtagacgatc ttcaacatga tcaatgacac tgcactgccc
     2761 aacgcgccca acgccgcccc ttcaagcaga tagggtacac gaataaaggt cgtggtcgct
     2821 ccgatgagcc gaagtatggc caattcgtca cgacgtgcat agagcgtgag ccggatcgta
     2881 ttcccgataa tcgtcaccgc cgcaaccgac aacacgactc ccacaccgat ggcgatcaac
     2941 tcaatggacc gaatgaaggt ggccaaggca ttgatccaat cctgattata atcgaccttc
     3001 gcgatccctg taatggtccg gacacgttcc gcccagcgcc tgacggcatc cggtgcgcga
     3061 tattgcgggg ataaggtgac gacaaacgag gccggcaacg gattctggcc caacccctcg
     3121 agtaaatgcg actctgatgg aaactgtgct ttgaattcac cgagtgcctg ttctttggaa
     3181 ataaacatca gcgatgcgag cgcatgatcg cttcgtaact gctgttcgat cgccgccgcc
     3241 gattcccgcg agacccgatc atcaagatag accataatct tcacgtcttc ctggagccag
     3301 ccggccgcgg cgcgaagatt cacgtagagc agcagaaaaa tcccgacaca ggccagcgta
     3361 aaggctgtgg tcaggatggc caccatcgtc gtggtccggt tggtccggag attggcccac
     3421 gcctcccgta acaggtagaa gaccgtcctc atgcccatac ccgctgttcc ggcaccaaca
     3481 ttccctggtc cagcttgacc acccgtcgat tgacttgcgc catgacgagc ggatcgtggg
     3541 tggctacgac gaccgtcgta ccacgggcgt tgatgagctt gaagagttcc atgatctcgc
     3601 tggtgagtcc gggatccaaa ttcccggtcg gttcgtcggc cagcaacacc accggcccgt
     3661 tcacaatcgc ccgggcgacg cagacgcgct gttgctctcc cgttgagaga ccgatgggca
     3721 attggtctcg cttgtggtcc acaccgaccg cccgcaatgc ctccgtcact ttgcgacgga
     3781 tgtcgaccgg cgaggctccc tgcaccagca gcggcagcgc cacgttgtca aagacggact
     3841 ttttcggtag taggcggaag tcctgaaaca cggtcccgat cttccggcgc aggtagggaa
     3901 tttcggacga cttgagtttc gtgacgttct tgccctggac aaacacatgg ccatcgtccg
     3961 gctgttcggc tccgatcaac attcgaagca gcgtcgactt cccggatccg ctcggcccca
     4021 tcagcaacac aaactcgcct ttttcgatgt cgagggtgac atcggcgagc gcggctctcc
     4081 gattatagta ctttgagacg tgaatcagtt gaatcatcag gggctcacca ccggagatcg
     4141 tctccggaca cttcaaaggc attttgaata tggtcgactt gagcgtcgtt tgcttccgtc
     4201 ccctgaacca atacgacgtc gaaccggcac ggccgattgg tgatgtgatg gcgggccaga
     4261 tactgggcgg ccaattggat cagcttgtcc tgcttgcgtc gatgcacggc atggacagcg
     4321 ccgccgtagg catcgctgtg gcgtgatttg acttcgacga acaccagcac ggcgccatcc
     4381 tccattacca agtccaattc ccccaccgag gaacgtgcat tccgttcgag gacccgatag
     4441 cccttccgac gcaaataccg ttccgccact gattctcctt cttgcccaaa gagtcgccgg
     4501 gggtcaagca ttgccgctca atcgtctgta ggatcgatcc tacgcttcag ccggtgaggt
     4561 gtccctcacg acacccgtcc tgccaataca gccgcgaccg gcgcgaacgt gcgccgatga
     4621 atcgaacagg gaccgtactc ggccagccgc tgtaaatgtt cttccgttcc gtatccctta
     4681 tgggaaagaa aattgtactg cgggtaggtt cgatgatagt ccgccatcaa tcggtcccgc
     4741 gtgactttcg ccacgatcga cgcggcggct atggagcgtg acagggcatc gcccttgatg
     4801 atggcgcgca ggggaatgtc gacgcccgga agttccatcg catcggtcaa cagacaatcc
     4861 ggcgtcacca agagcgcatc gaccgcccga tgcatcgccg ttcgcgtggc ttcgagaatg
     4921 ttcagcgtgt cgatttcctg ctccgtggca atcccgactc cgaccccgat cgcccgccgg
     4981 acgatcatgt catagagccc ttcccgttcg gactctgaga gctgtttgga atcgttgatt
     5041 cctgtgaggc agcagcgtgc ggggagaatg actgcggctg ccacgacagg acccgcgagg
     5101 ggacctcgac cggcttcgtc gagaccggcg atgcgccgat agccgcatcg ccgagcttct
     5161 acttcaaaat cgtcggtggg ttccacgagg tggcccagca ggatgttgaa cagtccgcca
     5221 gcgtggtcgc gaattattcg agagcgcccg taactatgat ttcgcagccc ctgccggctc
     5281 cgccgcttct tcggagagtt gctccgtctg ggcggctccg ggctggcctt tcgactctcc
     5341 cacgaactcg cgatcttcga ccttggcaaa cttgcccttt ttcccgcgaa gataatacaa
     5401 cttggcacga cggacttttc cttggcgtac gacctcaatg cgggtcacga tcggtgagtg
     5461 cacggggaaa atccgctcca cccccacgcc ataggatacc ttgcgcacgg tgaacatctc
     5521 ggtgttcagc gttcccttgc gggcgatcac cacaccctcg tacacctgga tccgttcttt
     5581 ttctccctcg atcactttca cgtggacacg gaccgtgtcc ccgatttcaa aacgagacag
     5641 cgattttttc gtcaacgagc gttggattcg ttcaagccga ttcatggtgt gatcctcccc
     5701 tccccacaac gaacaggcgc agatcctacg ccctcgcgaa taagttcatc caatagttgt
     5761 cgatcttctg cattcaatac ccgatccctc aagagatccg ggcgcctgcg gtacgtgttc
     5821 cgcagggctt ctttccgccg ccatagccgg atcgcttcat ggtggccgga gagcaagacc
     5881 tcgggaacgg tcatcccccg cacctccgat ggcctcgtgt agtgcgggta ctctaacgtc
     5941 gagtctgaaa acgattcctc gacgatggac tccggatcgc ccaacacacc gggaaccaat
     6001 cgagcggccg catcgatgag gatgagcgcc ggcaattctc caccggtcag gacatagtct
     6061 cccaccgaaa tctcctccgg ctggagcgcc agccgcaccc gttcatcgat cccttcgtaa
     6121 tgcccgcaca ggatgatgag ccgtcgaccc tcggcggcga gattcttcgc atactcctgg
     6181 gtaaagggcc ggccttgcgg agacggaaag agcagccgtg tgtgttcccc ggcacgttcg
     6241 tactcggccg tgatcgtctc gacggcacgc aggatcggct cggccttcat caccattcct
     6301 gcccctccgc catagggcac atcgtcagcg actttgtggc gatccagggt atagtcacgc
     6361 aggttcctga cgcgcacctc gagcagcccc ttctcttgcg cgcgcttgag catgctctgg
     6421 tcgaacaccg gggcgatcat gtccgggaac aatgtcagaa cgtcacaccg catcgtggta
     6481 ctcaatcaac ccctcaatgg cgcggaccgt catacgacgc cctgccacgt caactttcgt
     6541 gatcaggtct ctggcggcgg gaatcaacac ttcttgagaa ccttttcgaa caaccagcac
     6601 ataattcccc ggcacgtccc agatcgactc gattgtcccg atttctgctc ccaggtcgct
     6661 ctccaccgtc atcccgatga gatcacattc gtagtacgtg tgctccggcg ccacggaggg
     6721 gatcgtacgc ggcacttgaa tcagtcctcc gcggaacgct ccggcttcct caggcgaggt
     6781 aatgccttcg agacccatga tgaaacccgg accagccggc cgcacgtgcg tgaccgttgc
     6841 ctcgattgct ttcccatccg acaccacgac gctgactttc tcgaggtgat cgaatcggcc
     6901 ggggacatcg ctcaacgacc gaaccttgac ctctcccttc acaccgaagg gtcgttcgat
     6961 ctttccgatg gtgactcggt ccgccgacgt cgcttccata cgtctcacgc actcttcttc
     7021 ttggccgcct tctcaccctc gaattgcttc cacagcccgg accgtcgcag taacgtccgc
     7081 accgtcacgg tcggttgtgc gccctggcgc aaccaggtga ggacacgatc ctgcttgagc
     7141 tcaggcaccg ccggctcctt caacggatca aaggttccga ggatttcaag aaaccgcccg
     7201 tcccggggct tccgcgaatc agccgccacg actcgataga gcggccgctt gtgtcgtcct
     7261 gttctcgtta gtctcagatg aactgccact gtcttcctcc ttcgttgacg agacccctct
     7321 cgtttgcttc gcctacattg agcgcaatag ttgcgccagt tgcctccgtc ctcccgtccc
     7381 tgccatcgcc ttcgccatct tcctcgcacc gagaaactgc ttaatcaatc gattgacttc
     7441 gggcacgctg gtcccgcttc cccgggcgat ccgtttcttt ctgctgccgt tgatgatggt
     7501 atgatcgcgt cgttcacgcg aagtcatcga atcgatcatc gcggccaccc gcttcatctc
     7561 tcgttccggc agctgccctt ccatcgcccc cttgagcttt tggccgcccg gcagcatccc
     7621 cagaatctgg tccaaggagc cgaggcgatt gacctgcccc aa
//
LOCUS       DATDNW010000324         3555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_113448, whole genome shotgun sequence.
ACCESSION   DATDNW010000324 DATDNW010000000
VERSION     DATDNW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113448"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VI359_07795"
     CDS             complement(<1..242)
                     /locus_tag="VI359_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974192.1"
                     /translation="MAWGPDPNTEAELEILAAVREYLHEQFPGASIRDSYDAGRMAQV
                     FRIETDGVGGLRDAVLSTEFLEQYPPEKMGKALTGLR"
     gene            complement(519..1298)
                     /locus_tag="VI359_07800"
     CDS             complement(519..1298)
                     /locus_tag="VI359_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6974193.1"
                     /translation="MAQTKILVVEDEPIIARDIAETFTRLGYTVTAIASSGEEAVKKV
                     VEQQPDLVLMDIVLKGSMDGIETTACIQARYDIPFVYLSAYSDSEVLKRARETYPYGY
                     LIKPVTEHELAMAIERALFNHETNQWIKGHEPWAAWALHGNRDGVITTDEQGDVKFMN
                     PVAERLTGWFQVDALGKNLAQALELFHKESQAPFADAVRQVLEASREVVRLGDPIHLR
                     AKDGREHQITGSVASIRDEQGAVVGAAVIFREFMGVKKGKG"
     gene            complement(1305..>3555)
                     /locus_tag="VI359_07805"
     CDS             complement(1305..>3555)
                     /locus_tag="VI359_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6974194.1"
                     /translation="TLRLVGIILVLASTYTLAGKLGLSLAVVHPSATAVWPPSGIALA
                     ALLVLGYRVWPGILLGAFLVNITTSGNAVSSCGIAAGNTLEGLVGAYLVNRFANGPHF
                     CERPQDIFKFAVLAGIVSTTVSATVGVMSLALGGLASSADYPSIWLTWWLGDAMGDFI
                     IAPLLVLWTANPRIRWNRQEVMEAAFLLLVLFFVGQAVFGGWLALNGKHYSLDFLCIP
                     VLVWAAFRFGPRETATVAVVLSGIALWGTVRGFGPFVTGSRHESVLLLQMFMGTTAIM
                     TLAFAAVVLERKRAEETRARLAAIVESSDDGIIGKTLDGTVVTWNRGAERLFGYLAEE
                     VIGKPITVVIPPDRLHEESQIIERIRRGERLDQFESVRWRKDGQKIYVSLRISPIKDS
                     TGEIIGISKIVRNITERKQAEEQFRLMVESSPGGMLMVDQTGAIRLINAQLEKQFGYS
                     RQELLGQPVELLVPERFRAQHSTLRANFFASPSVRAMGAGRDLFGVRKDGTEFPIEIG
                     LNPLKTADGPRVLASIVDITERKRTEDQIQASLKEKEVLLREVHHRVKNNLQVVYSLL
                     RLQSKSVTDPKALELFQDSQARVKAMALLHEILYQSQDLSRIDMAGYISKLTGELFKS
                     YGISPNTISLRLNIAPVSLSVETAITCGLLINELVSNSLKYAFPNGRTGEVAIQLTSN
                     HNGAYTMTVGDNGIGLPPDVDVAAVQSLGWQLVPMLVEQLTGTFELERNAGTTVKLTF
                     SELSYKGRV"
BASE COUNT          736 a         1075 c          965 g          779 t
ORIGIN      
        1 cgcaagccgg tcaacgcctt gcccattttt tcaggcggat actgttcgag gaattcggtc
       61 gagagaaccg catcacgcag cccaccgacg ccgtcggttt cgatccgaaa tacctgtgcc
      121 attcggccgg catcgtacga atcgcgaatc gaggcgccgg ggaactgttc gtgaagatac
      181 tcacgcacag ccgccagtat ttccagttca gcttctgtat tcggatccgg tccccacgcc
      241 atggcttgcc ctcctctgtg tcccgctggt ggcaatctcc tctgcgtgcg tcacacacat
      301 atatgatggt ccttcagagc ttgcttgctc tctcctcaag gagtggtctg gatctatccc
      361 gtattgcgcg catcgagcga caaatataaa tggtccctcc aagcttgctt gcctgtctcc
      421 acacggggcg gcctggttaa tcctcaaatg cgcgcgtcga acgaggccct tccgagggcg
      481 cgcgttccgc gagcaggagg atgaccaggc cgccccgctc atccctttcc ttttttgacc
      541 cccatgaact cccggaagat aacggccgcc cctacaactg caccttgctc gtctcggatg
      601 gaggccacac tgccggtgat ctgatgctcc ctcccgtcct tcgctcgaag gtgtatcgga
      661 tcacccagtc ggacgacttc cctgcttgct tccaggactt gcctcaccgc atcagcaaac
      721 ggagcctgac tctctttatg gaagagttcc agcgcctgag caaggttctt ccctaaggcg
      781 tcaacttgaa accaccctgt gagtcgttct gccacaggat tcatgaactt cacgtctccc
      841 tgctcatcag ttgtgatcac gccgtccctg ttgccatgta gggcccacgc ggcccatggt
      901 tcgtgcccct tgatccattg gttggtttca tggttgaaca aggccctttc gatcgccata
      961 gccagctcgt gctcggtaac gggcttgatg agataaccat atgggtaggt ctcccgagcc
     1021 cgcttgagga cctcgctgtc gctatacgcc gacagataga caaacgggat gtcataccgt
     1081 gcctggatac atgccgtggt ttcaatgccg tccatgctcc ctttcaggac aatgtccatc
     1141 aagaccaagt cgggctgctg ctccacaacc tttttgactg cttcctctcc tgacgatgcg
     1201 atcgcggtga ctgtatagcc caagcgtgtg aaagtctccg caatatctct ggcgatgatg
     1261 ggctcgtctt cgacgacgag aatcttggtc tgggccatgg cgttctagac ccttcctttg
     1321 tagctgagtt cggagaacgt gagcttcacc gttgtgcctg cattccgctc taactcgaag
     1381 gtccccgtga gttgctcgac caacatcggg accagttgcc accctaagga ctggaccgcc
     1441 gccacgtcta cgtccggtgg caagccgata ccattgtcac caacggtcat cgtatatgct
     1501 ccattgtgat tggacgtgag ttgaattgcc acttcccccg tgcggccgtt tggaaaggcg
     1561 tatttcaaac tgttggacac cagttcgttg atcagcaatc cacacgtgat cgcggtctct
     1621 acgctgagag acacaggcgc aatgttgagt ctgagactga tggtgttggg tgaaatccca
     1681 taggacttga acaactcccc ggtcagcttt gagatgtacc ctgccatatc aatccgtgag
     1741 aggtcttgcg attgatagag gatctcgtga agcaatgcca tggctttgac ccgcgcctgg
     1801 ctgtcttgga acagctccag ggctttcgga tcagttactg atttcgactg cagtcggagt
     1861 agactgtaca cgacctgcaa attgttcttg acccggtggt gcacttccct caacaagacc
     1921 tccttttctt tcagcgacgc ctggatttgg tcctccgtcc gcttgcgctc ggtgatgtcc
     1981 acgatcgagg cgaggacccg tggtccgtcc gcagtcttca gggggttcag cccaatttca
     2041 atcggaaact cggtgccatc cttgcgcact ccaaacagat cgcggcctgc ccccatggca
     2101 cgcacggaag gactcgcgaa aaagttggct cgcagtgtgg agtgttgggc ccgaaaccgt
     2161 tccgggacca ggagctcgac aggctgcccg agcaattcct ggcggctgta cccgaactgt
     2221 ttctccagct gtgcgttgat cagccggatg gcgccggtct ggtccaccat cagcatgccg
     2281 cctggggagg attccaccat caagcggaac tgctcttcgg cctgtttacg ctcagtaatg
     2341 ttacggacga tcttggagat gccgatgatc tctcctgttg aatccttgat tggcgaaatc
     2401 ctcaaggaga cgtagatctt ctggccgtct tttcgccacc gcacggattc aaattgatcg
     2461 aggcgctcac cccggcggat gcgttcgatg atctgagact cctcgtgcaa gcggtcgggc
     2521 gggatgacga ccgtgattgg cttcccaatc acttcctcgg ctagatagcc gaagagtcgc
     2581 tcggcacctc tattccatgt gaccaccgtc ccgtcgagag ttttgccaat gatgccgtca
     2641 tcggaggatt ccacgatggc ggcgagtcgg gcacgtgtct cctcagcccg cttgcgttcc
     2701 aacacgacag ccgcaaaggc aagagtcatg atcgcagtgg tccccatgaa catctgcaac
     2761 aacaggacgg attcgtgccg cgaacctgtc acaaagggac cgaagccgcg cacggtgccc
     2821 cagagtgcga tgcctgacag cacgacggcg actgtggctg tttcacgggg gccgaatcgg
     2881 aaggccgccc agacaaggac tgggatacac aagaaatcga gcgaataatg cttcccattg
     2941 agagcaagcc atccaccgaa gacggcctga ccaacaaaga acaggaccag caagaggaag
     3001 gccgcctcca tgacctcctg tcggttccag cggatgcggg gattcgcggt ccagagcacc
     3061 aacagcgggg cgatgatgaa atcgcccatg gcatcgccga gccaccaagt cagccagatg
     3121 gatgggtagt cagccgaact ggcaaggccg cctagagcaa gactcatcac accaactgtg
     3181 gcactcaccg tcgtgcttac tatcccggca agcaccgcga atttgaaaat atcctgcggc
     3241 cgctcgcaga agtgaggacc gttcgcaaac ctattcacca ggtaagcgcc caccagtcct
     3301 tcgagcgtgt tgcctgctgc gataccacat gaggagacgg cgtttcccga ggttgtgata
     3361 ttgacgagaa atgcgcccag gagaatgccc ggccagacgc gataccccag gaccagaagg
     3421 gccgccagcg cgataccgct aggcggccat accgccgttg cgcttggatg cacaacggct
     3481 agactcaggc cgagtttgcc tgccagggta taggtactgg ccaagacgag gattatccca
     3541 accagtcgga gggtc
//
LOCUS       DATDNW010000325         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_872620, whole genome shotgun sequence.
ACCESSION   DATDNW010000325 DATDNW010000000
VERSION     DATDNW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872620"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1963
                     /gene="dnaK"
                     /locus_tag="VI359_07810"
     CDS             44..1963
                     /gene="dnaK"
                     /locus_tag="VI359_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY6974195.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVAITD
                     KGERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSREVQEAIKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSVLEIGEGVFEVKSTNG
                     DTYLGGDDFDLRVMDWLVDEFRKDQGIDLRKDRMALQRLKEAAERAKIELSSSQETEI
                     NLPFITADASGPKHMVIKLTRAKLEQLVDDLIQRTVDPCKKALGDAGVSARDINEVVL
                     VGGMTRMPKDIQVVKEFFGKEPHRGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQVEVTFDIDANGIVHVSAKDMATQKEQSIKITASSGLSKEEVE
                     KLVRDAQSHTEDDKKRRKAAEAKNQADNLIYQTEKNLAEHGDKVPAEEKTKIEEAVAA
                     LKKAMEGTDTAAVESATQTLMAASHKLAEEMYKKASATSGAGASTAAGNGAGETKTDE
                     KVVDAEFEEVDKDKK"
     gene            2048..3157
                     /gene="dnaJ"
                     /locus_tag="VI359_07815"
     CDS             2048..3157
                     /gene="dnaJ"
                     /locus_tag="VI359_07815"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY6974196.1"
                     /translation="MSKRDYYETLGVDRTASDDELKKAYRKLAREHHPDLHTGDQQKK
                     RAEEKFKEINEAYETLSDQEKQRRYDMFGHAGAQQGGGFEGFGGGGFGDVFNDIFDDF
                     FGGQRGSSRAERGNDLQYNLELTFEESVYGKEAKLKIPRWETCGECQGTGAKSSAAIK
                     TCPSCKGAGQIRLQQGFFSISRPCGQCEGSGRIVTEPCPACQGRQRTYRERTIAVHIP
                     AGIESGMRLRLANEGESGVNNGPSGDLYVAITVKPHPVFQRKGHDIVCEVPVHFVTAV
                     MGGKVEVPTLKGNTVIKIPPGTQHDKVLRLKGLGIPSLKGHSTGDQQFVIKVQIPTKM
                     TARQKELLTEFAKESGMSMDAEGDGFFDKMKTFFE"
     gene            3200..3940
                     /locus_tag="VI359_07820"
     CDS             3200..3940
                     /locus_tag="VI359_07820"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY6974197.1"
                     /translation="MPTFFIAPETITLPTIQIAGPLLHHLRESLRLHRGESLILTDHQ
                     GIRYHVEVTDITSRAIHARVVNQETAPPRSTPQIVLGQALLKGEKMDWVLQKATELGV
                     DTIVPLETHHSVVKPNPERLEHQRNRWRRIVLEAAQQSERWTIPQVSDPTSLARFVDQ
                     QSGATMKGILSERSKGSSLISLALPREPHHVIVLLTGPEGGWTPEEQQLARDWGFVAL
                     TLGGRILRAETATLAALSILQSRLGELG"
BASE COUNT         1013 a         1174 c         1185 g          710 t
ORIGIN      
        1 ttacagttcg aacaattgat cgcttggagg aaggagcgca cacatgggta aagtaatcgg
       61 catcgacctg gggacgacca actcctgcgt cgccattatg agcgggggag atcctgtcgt
      121 cattgcaaac gcggaaggaa gtcggacgac gccgtccgtc gtggcgatca ccgacaaggg
      181 agaacgtctg gtagggcaga tcgcaaagcg gcaggccatt accaatccgg agaacactat
      241 cttttccgtg aaacgcctga tgggccgcaa gttcaagagc cgcgaggtgc aggaagccat
      301 aaaacgcctc ccgtacaagg tggtggaagc cgacaacggc gatgcgcacg tcgagttgcg
      361 cggtaaacgg tacagcccac ctgaaatctc tgccatgatt ctccaaaaaa tgcgacagac
      421 cgcggaggat tatctcggcg aaaaagtcac ggaggccgtc gtcaccgtcc cggcctattt
      481 cgacgacagc cagcgtcaag ccaccaaaga cgcgggccag atcgccggcc tcaacgtcct
      541 gcgtatcatc aacgagccta cagccgcctc tctggcctac ggcctcgaca agaaaaaaga
      601 cgagcgcatt gccgtctatg atttaggcgg cgggaccttc gacgtctcgg tcctggaaat
      661 cggcgaaggc gtgtttgagg tgaagtcaac aaacggcgat acctatctcg gcggcgacga
      721 cttcgacctg cgagtcatgg actggttggt cgacgaattc aggaaggacc agggcatcga
      781 tctgcgcaaa gaccggatgg cgctgcagcg gctcaaggaa gccgccgaac gggccaagat
      841 cgaattgtct tcttcgcagg agacagaaat caatcttccc ttcatcacgg ccgatgccag
      901 cggcccgaaa cacatggtca taaaactcac tcgcgccaag ctggaacagt tggtggacga
      961 cttgatccaa cgcaccgtcg acccctgcaa gaaggccttg ggggacgccg gcgtctcagc
     1021 tcgtgacatc aacgaagtgg tgctggtcgg aggcatgacc cgcatgccaa aagacattca
     1081 agtcgtgaag gagtttttcg ggaaggaacc gcatcgtggc gtgaacccgg acgaagttgt
     1141 ggcgatcggc gccgcgattc agggcggcgt cctcaagggc gacgtgaaag acgtcctcct
     1201 gctcgacgtc actccgcttt cgttgggcat cgaaactctc ggcggcgtct tcacgaagct
     1261 gatcgagcgg aatacgaccg taccgaccaa aaagagccag gtgttttcga cggctgccga
     1321 caatcaaact gccgtcacga tccgcgtttt ccaaggcgaa cgcgaaatgg cgaacgacaa
     1381 taaactgctc ggtcagttcg acctcgtcgg cattccgccg gctcctcgcg gtatgccgca
     1441 ggtcgaagtc accttcgaca tcgatgcgaa cggcatcgtg catgtttcgg cgaaggacat
     1501 ggcgacgcag aaggaacagt ccatcaagat caccgcgtcc agtggactga gtaaagagga
     1561 agttgagaag ctcgtcaggg atgcccagtc gcacaccgaa gacgacaaga aacgccgcaa
     1621 ggcggcggag gctaagaatc aggccgacaa tctgatttat cagacggaga agaacctcgc
     1681 cgagcatggc gacaaggttc ccgccgagga gaaaaccaaa attgaagagg cggtcgcggc
     1741 tctcaagaaa gcgatggaag gcaccgatac cgccgccgtc gaatccgcga cacagactct
     1801 gatggcggct tcccacaagc tggccgaaga gatgtacaag aaggcctccg ccacatccgg
     1861 tgcaggagcc tctactgccg ccggcaacgg ggccggcgaa acgaaaacgg atgaaaaggt
     1921 cgtcgatgca gaattcgaag aagtagacaa agacaagaag taagctacaa taccggcatt
     1981 gagaccgagt cccgttcatc aacggagctc ggtctttctg cgcttctcct cgagcaccgt
     2041 gagaacagtg agcaaacgcg attactacga aaccctcggc gtcgatcgga ccgcgtccga
     2101 cgatgagttg aagaaagcct accgcaagct ggcccgcgag catcatcccg acctgcatac
     2161 cggcgaccaa cagaagaaac gcgcggaaga aaagttcaaa gagatcaacg aagcctacga
     2221 aacgctcagc gaccaagaga aacagcgacg ctacgacatg ttcgggcatg ctggtgccca
     2281 gcaaggaggc gggtttgaag ggttcggtgg aggcgggttc ggcgacgttt tcaacgatat
     2341 cttcgacgat tttttcggcg ggcagcgcgg cagcagccgg gcggaacgcg gcaacgacct
     2401 ccaatacaac ctcgaactca ccttcgaaga atccgtctac ggcaaagagg ccaaactcaa
     2461 aatccctcgg tgggaaacct gcggcgagtg ccagggaacc ggcgccaaat cctccgctgc
     2521 catcaagacc tgtccgagtt gtaagggcgc gggacagatc cgtctccaac agggtttttt
     2581 cagcatcagc cgcccgtgcg gacaatgcga agggtccggc cggattgtga cagagccttg
     2641 ccctgcctgc caaggacgac aacggaccta ccgcgagcgc accattgcgg tccacattcc
     2701 ggccggaatc gaatcaggca tgcggctgcg gctcgccaac gaaggcgagt ccggtgtcaa
     2761 caatggtcct tcgggtgacc tctacgtcgc gatcaccgtc aagccacatc ccgtgttcca
     2821 gcgaaaagga cacgacatcg tctgcgaggt cccggtccac tttgtgaccg ccgtgatggg
     2881 cggcaaggtc gaagttccca cgctgaaggg caatacagtg atcaagattc ctccgggtac
     2941 acagcacgac aaggtcctac gcctcaaagg gcttggcatc cccagtctca aagggcatag
     3001 caccggcgat cagcagttcg tcatcaaggt gcagattcct accaagatga ccgcacgcca
     3061 gaaggaatta ctcacggagt ttgccaagga aagcggcatg tcgatggatg ctgagggtga
     3121 cgggttcttc gacaagatga aaacgttctt cgaatagccg tcttccttcg tcgtctcacg
     3181 caggccgcca ccgcagtcca tgccgacgtt tttcatcgct cctgagacca tcactttacc
     3241 gaccatccag attgccggtc cgttgctgca ccacttgcgc gagagtctcc ggctccatcg
     3301 gggtgaatcg ttgatcctga cagaccatca gggcattagg tatcacgtgg aggtcacgga
     3361 catcacatca cgggcaatcc acgctcgtgt cgtcaatcag gaaacagctc cgcctcgctc
     3421 gaccccacag attgtgctcg gtcaggctct gttgaagggt gagaagatgg attgggtgct
     3481 gcaaaaggcg actgagctcg gcgttgacac aatcgttccg ctcgaaactc atcacagcgt
     3541 ggtgaaacca aacccggaac ggcttgaaca tcagcggaac agatggcgtc gcatcgtctt
     3601 ggaagctgca cagcaatcgg aacgatggac gatcccgcag gtgtcggatc cgacatcgct
     3661 tgcacgattt gtcgatcagc agtcaggggc cacgatgaaa gggatcctgt cggaacgaag
     3721 caaggggtcc tccctcattt ctctcgcgct tccgcgcgag ccccatcacg tgatcgttct
     3781 tttgacagga ccggaaggcg ggtggacccc cgaggaacag caattggctc gagactgggg
     3841 attcgtcgcg ttaacactgg gtggacggat tcttcgagca gagacggcaa cactcgccgc
     3901 actcagcatt ctccaatcga gactgggtga attgggatag cagagaatct accgaccggt
     3961 cgaaagacga acgatagtgc ccttttcacc ggttgcccag ccatgcagcg catcggggaa
     4021 cgagagccca aagagcgaaa ccttggcgac gcttccccgt tccacccagg taaagccgct
     4081 at
//
LOCUS       DATDNW010000326         2551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1206068, whole genome shotgun sequence.
ACCESSION   DATDNW010000326 DATDNW010000000
VERSION     DATDNW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206068"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VI359_07825"
     CDS             <1..320
                     /locus_tag="VI359_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974198.1"
                     /translation="LGGETTLRAFGQSRFIDENALLVNFEERIPVLNKRIFDHPINLE
                     AAPFVDIGRVYDTFGTKSFTNLQVNPGLGLRMLAPPNVIGRLDIGHGRDGTNVFVGLD
                     YPF"
     gene            376..840
                     /locus_tag="VI359_07830"
     CDS             376..840
                     /locus_tag="VI359_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY6974199.1"
                     /translation="MMLRLLVAALLPFLALCSACTTASLLNAAGGGDTKSVVEILQEG
                     ADVNASFPVIGTRALMVAAAQGHVDAVKALLDAGADVNAADLTGWTALHAATYKGDKQ
                     IISLLLERGAIAPAPTWFLQRPSDMAEKLGHQDIVPLLKQVEAGNPRISTLP"
     gene            950..1147
                     /locus_tag="VI359_07835"
     CDS             950..1147
                     /locus_tag="VI359_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY6974200.1"
                     /translation="MKVQLSHPARTVDVKGPKRVNELLRELNLIVEAHLVIKGDDLVT
                     EDEMLYDQDQVEIRPVISGGM"
     gene            1218..2156
                     /locus_tag="VI359_07840"
     CDS             1218..2156
                     /locus_tag="VI359_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY6974201.1"
                     /translation="MNCTKCKTKAAIGLPRHNAAYCKGCFTQFVHDQVARAIKSQKMF
                     GKDDRILVAVSGGKDSLALWDILLKLGYRADALYVNLGIGTYSEDSYRKVTKFAESVA
                     SSHGAKLLTHTVEEEEGAGIKELAQLIHRPTCSTCGTIKRYQFNRAAVEHDYDVMATG
                     HNLDDEAARLLGNVLHWQDEYLDKQGPSLPASVEGFAKKVKPLYRLSERELAAYAVLN
                     RIDYIVEECPMAKGARTLVYKEVLNRLETESPGTKQSFYWGFLDKQNKRTSAPATMTE
                     KDQSTLHPCTTCGQPTTAEVCSYCKMMARAKTAASH"
     gene            2225..>2551
                     /locus_tag="VI359_07845"
     CDS             2225..>2551
                     /locus_tag="VI359_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY6974202.1"
                     /translation="MAATERDYYHTLGLARSASAEEIKKAYRRLARQYHPDLHSGAKK
                     TEMEKKFKEVNEAHEVLSDPDKRKKYDQYGAQWEQADAFERARQQAGARGEGGPEFSF
                     GGEGFSD"
BASE COUNT          597 a          743 c          693 g          518 t
ORIGIN      
        1 cgttgggcgg agagacgact ttgcgggcgt ttggccagag ccggttcatc gatgagaatg
       61 cgctcctggt caattttgag gaacggatcc ctgttcttaa taagcgaata ttcgaccatc
      121 ctatcaatct cgaggcagcc ccctttgtag acattggacg ggtgtatgac accttcggca
      181 ccaagtcctt cacgaaccta caagtgaatc ctgggcttgg cctccggatg ctcgctcccc
      241 ccaatgtcat tggtcgactg gacatcgggc atgggcgcga tgggacgaac gtcttcgtgg
      301 gcctggatta tccgttctaa tctttacggc attctggaat ttctctcgta tgactgacgt
      361 gatcggtgag actagatgat gctccgactt cttgttgccg cacttctgcc cttcctcgca
      421 ctgtgttcgg catgcacgac cgcctccctg ctaaacgccg cgggtggcgg tgatacaaaa
      481 tcggttgtgg aaatcctcca agaaggagcc gatgtaaacg cgtcattccc cgtcatcggg
      541 actcgcgccc tcatggtcgc tgccgcgcag ggccacgtcg atgccgttaa ggcattgctc
      601 gatgcgggag ccgatgtgaa tgccgcagac ctcaccggat ggactgcgtt gcacgccgct
      661 acctataagg gtgataaaca gatcatctca ctgttgctgg agcgcggggc catcgcacct
      721 gcaccgacgt ggtttttgca gcgcccctcc gacatggcag agaagttagg gcaccaagac
      781 atcgtgccgc tcctcaagca ggttgaggca ggcaacccac gaatttcgac cctcccttga
      841 gcagcggata gttgctgtgt aagccagtaa agtacaaccc tcttgctgtc tctaatgacg
      901 gtttaaccca tttgcaatca tctatcgacc actcgtatca tcgtgcacga tgaaggtcca
      961 actcagccat cccgcaagaa ccgtcgacgt caaaggcccg aagcgggtca acgaactctt
     1021 gcgcgagttg aacctgatcg tcgaagcaca cctcgtcatc aaaggggacg acctcgtcac
     1081 cgaagacgag atgctatatg atcaggacca ggtggagatt cggccggtca tttcaggagg
     1141 aatgtagctc tcagtcttca gctttgagca gtcagcttca agagcctgac aactgaacag
     1201 cggagggcta cgtcaccatg aactgcacca agtgtaaaac caaagccgcc attggtctcc
     1261 cgcgtcacaa tgccgcctac tgtaaaggct gcttcacaca attcgtccac gaccaagttg
     1321 ccagggcaat caagtcacaa aagatgttcg gaaaggacga ccgcatcctc gtcgccgtct
     1381 cgggcgggaa ggacagtttg gccctctggg acattctgct caagctcggc tatcgcgccg
     1441 atgcactcta cgtgaatctt ggcatcggga cctattcgga ggactcgtac cgcaaggtca
     1501 cgaagttcgc ggaatcagtg gcgagctcac acggcgccaa gttgctgaca cataccgtcg
     1561 aagaggaaga aggggctggt atcaaggaac tggctcagct gatccatcgc ccgacctgct
     1621 cgacgtgcgg gacgattaaa cgctatcaat tcaatcgcgc tgctgttgaa catgactacg
     1681 acgtgatggc caccggacat aacctggatg acgaggccgc tcgcctgctc ggcaacgtgc
     1741 tccattggca agacgagtat ctcgacaaac aggggccgtc gctcccggcc tccgtcgaag
     1801 gattcgccaa gaaagtgaaa ccgctctatc gcctctcgga acgggagctc gccgcctatg
     1861 ccgtgttgaa ccggatcgac tacatcgtcg aagaatgtcc catggcaaaa ggggcgagaa
     1921 ctctcgtcta taaggaggtt ctcaatcggt tggaaaccga atcgccgggc acgaagcagt
     1981 ctttctattg ggggtttctc gataaacaga ataagagaac ctcggctccg gcgaccatga
     2041 ccgaaaagga tcagtccacc ctccatccct gcaccacctg tggtcagccg accaccgccg
     2101 aggtctgctc ctactgcaag atgatggcgc gcgccaagac cgcggcttct cactagtcca
     2161 tcgtcatgac ccttgcaaag tgtgtaggct ccccgtaagc tgaaaaacta tcggattctg
     2221 aatcatggcc gcaaccgaac gcgactacta tcacactctc ggccttgctc gcagcgcgtc
     2281 cgccgaggag atcaagaagg cctatcggcg tctggcccgc caataccatc ctgatctcca
     2341 tagcggcgcc aagaaaaccg agatggaaaa gaagttcaaa gaagtcaacg aagcccatga
     2401 agtgctctct gatcccgaca aacggaagaa atacgatcag tacggcgcgc aatgggaaca
     2461 agccgacgcc ttcgagcggg cacggcagca ggccggcgcg cgcggcgaag gcggaccgga
     2521 attttccttc ggcggcgaag ggttttccga t
//
LOCUS       DATDNW010000327         4813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_178650, whole genome shotgun sequence.
ACCESSION   DATDNW010000327 DATDNW010000000
VERSION     DATDNW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178650"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1266
                     /locus_tag="VI359_07850"
     CDS             103..1266
                     /locus_tag="VI359_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HEY6974203.1"
                     /translation="MIHGWFPTRWLIGMVFIMPAFAGCSWFGGQAKPDWIDGVSRAYP
                     AEQYLLGVGQSDNRATAEDQAYAAVSRIFKAEVSAQAKDWESYLVIEQRGQSSEQRRL
                     ALDNLTRVSTDKVLENVRILDRWADIRKGLHYALAGMQRAQSEAAILERIRELDRTVQ
                     EEVDQAHQSTDKLVKVKGLRRAAKNLVLRETYNADLRVIRPSGQGTAAAYRVNELTSE
                     LEQFLATNLVLALSVGGDQVEPVQRALTEGLIREGLQVTTRPFEAADSMSAGSSGPSA
                     ELLIRGLVRVWPIDVRDPQFKYVRWCSDFEVIDLTAQRVVGALSKGGKEGHLTEREAT
                     AKVIRVMQQDMSTDVAKAIAAHVYGETELPAQAIPPAGCPREGQSSKPAAATH"
     gene            1293..1553
                     /locus_tag="VI359_07855"
     CDS             1293..1553
                     /locus_tag="VI359_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974204.1"
                     /translation="MGTGRASRAPRSRGRGLTVTQQRVPSRLAKRRLGERLREDTVLF
                     NKGNLAEKLRKEGYEESPLDELQDRLSKMPGPMAAVILKGRV"
     gene            1558..2001
                     /locus_tag="VI359_07860"
     CDS             1558..2001
                     /locus_tag="VI359_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY6974205.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAILPTWTVTD
                     FYSVLSNRAQSGEITRDDCYSVIYKFEIESQQGLYQFLTPTMQTYLATKELALEYPFL
                     RSPQVLHLALALELKPLRLTVVSSDSQLLTASRSAGLHIINPEED"
     gene            complement(2095..2628)
                     /locus_tag="VI359_07865"
     CDS             complement(2095..2628)
                     /locus_tag="VI359_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974206.1"
                     /translation="MANRSFVATKRGRALVVLAGIFVWHTGTVGWSQVVGDEAEMDRL
                     KAKAEDLMANDDPEGAAMSMGRAALMAKQLAMKHKDAAASARIFQGAERLFRSQEHAY
                     RAMALFRRAGGQLPASSGVCGSLALAKSNVQQATSLLEQNGNVTGPPAERAVHLRATA
                     DDWITVIDSMISDYQCP"
     gene            complement(2644..3717)
                     /locus_tag="VI359_07870"
     CDS             complement(2644..3717)
                     /locus_tag="VI359_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6974207.1"
                     /translation="MKQLTVWTVVFLILLATTSWARQESYTPEQKAKLEKADRVLVEV
                     IALSDKGSIDHGALNDVVIKRMKELGYTMVTDPTQAHDVVFKVKCEQRKTWEGTTNMG
                     SDADQPDSPARVWKGPACQLTYFLEGKKMGWRKEVRTDFVDAQQAADAAKAGDPTAYA
                     MSKLRERLEDYDFPALLTAEWGQEDRLFKLYDDPATPPARKVKLIGLFGYLFSTKAVP
                     RLLTGLNGNDMEIAKASALALGNIGQKDTIPILIQTMKNGRPELRPSAAKALGVLGAL
                     HGDFTIVDPLLETLKTDDVVLKTEVVWALGKLPDRRAHEPLLALQKSLYHVRENDADP
                     KLVKLKEAVNWSIKQIDTWEYLQ"
     gene            3849..4667
                     /gene="proC"
                     /locus_tag="VI359_07875"
     CDS             3849..4667
                     /gene="proC"
                     /locus_tag="VI359_07875"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY6974208.1"
                     /translation="MLEDVRIAFIGAGNMAEAMIAGLLHARLMRPGQLVASDIVPRRL
                     DWLKRTYGVETTGSNREAVEGAQVIVLAVEPQILDEVLGEIAPSIDSRVLIVSVAAGY
                     PIARIARLLNGLGRIVRAMPNTPSIIREGVTALTPATGLSEKDLAIVQAMFEVIGTVV
                     VVPERSMDAVTGLSGSGPAYVYIMIEALADGGVKMGLPRETAQLLAAQTVAGAARLVL
                     ESHEHPGVLKDRVASPGGTTIAGLYELERRCFRATMISAVEAASRRSAELGMVS"
BASE COUNT         1032 a         1273 c         1462 g         1046 t
ORIGIN      
        1 cggaggatct ggtcaatcga atgattccac ggggacgaag acggcacgaa acctcacgct
       61 tggcgcggga gaaacggtct ttctccttca acgggtgatg ccatgataca tggttggttc
      121 ccgacacgtt ggttgatcgg catggtgttc atcatgccgg ccttcgccgg ttgttcgtgg
      181 tttggaggtc aggccaagcc ggactggatt gatggggtga gccgagccta tccggcagag
      241 cagtatctcc tcggtgtcgg acagtcggat aaccgggcga cggcggagga tcaggcctac
      301 gcggcagtct cgcgtatttt caaggcagag gtcagcgccc aagcgaagga ttgggagtcg
      361 tacctggtga tcgagcagcg cgggcagagc agcgagcaac gaagattagc actcgacaat
      421 ctgactcgcg tgtctaccga taaggtgttg gagaatgttc gaatcttgga ccgctgggcg
      481 gacatacgca aagggttgca ctatgctttg gccggcatgc aacgcgcgca gtctgaagcg
      541 gcgattctgg aacgaatcag agaactggat cgcacggtac aggaagaggt cgatcaggct
      601 caccaatcaa cggataaact cgtaaaagtc aaaggactgc gccgggcggc aaagaatctc
      661 gtcctacgcg aaacctataa tgcagacctg cgggtgattc gtccgagtgg acagggaacg
      721 gcggcagcct atcgggtcaa tgaactcacg agcgagctcg aacaatttct cgcgacgaat
      781 ctggtgctgg ctctatccgt cggcggcgat caggtcgagc cggtgcagcg agccctcacc
      841 gaagggttga ttcgggaggg acttcaagtc accacccgtc cgttcgaagc ggccgattcg
      901 atgagcgccg gctcatcggg gccttcggca gagttgctga tccgaggtct cgtccgtgtg
      961 tggccgatcg atgtgcgcga tcctcaattc aagtacgtcc gatggtgcag cgatttcgaa
     1021 gttatcgatt tgaccgctca gcgcgtggtc ggggccctgt cgaagggagg aaaggaaggt
     1081 catctcacag agcgggaggc gacagcaaaa gtgattcgcg tgatgcagca agatatgtcg
     1141 acggatgtag cgaaggcgat cgcggcacac gtgtacggag agacagaact gccggctcag
     1201 gcgattccgc ctgcaggatg ccctcgagag gggcaatcat ccaagccggc cgcagcaaca
     1261 cactaagcca taagagaggg agggcttcgt cgatgggaac tggacgagca agtcgcgccc
     1321 cgcgcagtcg tgggcgggga ttgacggtga cgcaacaacg ggtaccaagt cggctggcta
     1381 aacgccggtt gggtgaacga ctccgggaag acacggtgtt gttcaacaaa ggtaatctcg
     1441 cagagaaact gcgcaaagag ggctacgaag agtcaccgtt ggatgaactg caagatcgcc
     1501 tctcaaaaat gccgggaccg atggcggcgg tgattttaaa ggggagggtg tgagcgcatg
     1561 ccgtactact atttcgactc gacggcctta gtgaaacgtt acagcatgga acgtgggacg
     1621 cgtatcgtga ataagctcat ggtcaagcga gggaaagtcg ccatccttcc gacctggacg
     1681 gtcacggatt tctattcggt cctttcgaat cgagcccaat caggggaaat cacgcgagac
     1741 gattgttatt cggttatcta taaattcgaa atcgaatcgc agcagggatt gtatcaattt
     1801 ctgacaccga cgatgcagac ctatctggca accaaagaac tggcgctgga gtatccgttt
     1861 ctacgttcgc cgcaagtgct gcacttggca ctcgcattgg aattgaagcc attacgattg
     1921 accgtggtca gttcggattc ccagttattg acggccagtc gctcggcggg gctgcatatc
     1981 atcaatccag aagaggactg agcgggcgag tgcagagtgg tgttcagagc cgagtactgg
     2041 gtgccgagag ttgaaaggtg atggctgaca actgaacgtc cgtgagctgg gttctcacgg
     2101 gcactggtaa tcggatatca tcgagtcaat cacggtgatc caatcgtcag cggtggcgcg
     2161 caggtgtacg gcccgctcgg ccggaggccc tgttacgttt ccattttgtt ccaacagtga
     2221 ggtcgcttgt tgcacgttgc tttttgcgag ggccagactg ccgcagactc ccgaggaggc
     2281 cggcagttga ccaccggccc ggcggaacag tgccatcgcc cggtacgcat gttcttgtga
     2341 gcggaagagc ctttcagccc cttgaaagat ccgagccgaa gcggccgcgt ctttgtgttt
     2401 catcgctaat tgcttggcca tcaacgcggc acgacccatg ctcatggcgg cgccttccgg
     2461 gtcgtcgttc gccatcaaat cctctgcctt ggccttgagt cggtccattt cagcctcgtc
     2521 accgacgacc tgactccaac cgaccgttcc tgtatgccag acaaagattc cggccagaac
     2581 gacaagggct cgacctcgtt tggttgcgac aaacgaacga ttcgccatgg ggataggatc
     2641 aacttactgg agatattccc acgtgtcgat ttgcttgatg ctccagttga cggcttcctt
     2701 cagtttcacg agcttgggat cggcgtcatt ctctcggacg tggtagagcg acttctgcag
     2761 tgccaacaac ggttcatggg cacgccggtc cggcagtttc cctaatgccc agacgacctc
     2821 ggtcttcagc acgacatcat cggttttcag cgtctcgagc aacggatcga cgatcgtgaa
     2881 atctccatgc aacgcgccga gcactcccag cgctttggcc gccgacggac gcagctcggg
     2941 ccggccgttc ttcatggttt gaatcaatat ggggatcgtg tctttctggc cgatgttgcc
     3001 caacgcaaga gcagaggcct tcgcaatctc catgtcgttc ccattgagtc cggtcaggag
     3061 tcgaggcacg gctttcgtgg aaaaaagata tccgaacaag ccgatcagct tgactttacg
     3121 tgccggcggt gttgcagggt cgtcgtacaa cttgaagagg cggtcttcct gtccccattc
     3181 ggctgtgagc aaagccggaa aatcatagtc ctccaaccgt tccctcagtt tgctcatggc
     3241 ataggcggtc gggtcaccgg ccttggctgc atcggccgct tgctgtgcgt cgacgaagtc
     3301 cgttctcacc tccttgcgcc agcccatttt cttcccttca aggaaatagg tcagttggca
     3361 ggcgggtcct ttccacacgc gtgccggcga atcgggctgg tccgcatcac tccccatgtt
     3421 cgtggttcct tcccaggttt ttcgctgttc gcatttcacc ttgaagacga catcgtgagc
     3481 ctgagtcgga tcggtcacca tcgtgtaacc aagctctttc atccgtttga tcaccacatc
     3541 attgagtgcg ccgtgatcga tcgacccctt gtctgagagt gcgatcacct ctaccaacac
     3601 ccgatcggct ttttcgagct tggctttctg ttcgggggtg taggattcct ggcgggccca
     3661 actggttgtg gccagcagta tgaggaacac aacagtccac acggttagct gcttcatgag
     3721 tgcctccacg tgccgaatat aatgagtcca cgagcacaat aatcctcgac atcactgtac
     3781 agaagagacg gcgaccacgg caagttgcaa gttgaagggg aggagaggcc gacagtataa
     3841 tcgcgcccat gcttgaggac gtccgcatcg cctttattgg ggcggggaat atggctgaag
     3901 cgatgatcgc cggcctcttg catgcgaggc tgatgcgtcc cggccaactc gtcgcctccg
     3961 atatcgttcc tcgccgtctc gattggctca aacgcacgta tggggtagag acgactggat
     4021 caaatcgaga ggcggttgaa ggtgcacagg tcatcgtcct cgctgtggag ccgcagattc
     4081 tcgacgaggt gttgggagaa attgctccga gtatcgactc ccgtgtcttg atcgtgtccg
     4141 tcgccgctgg atatccgatt gcgcgaattg cccgcctgtt gaacggactc ggacgcattg
     4201 tccgtgccat gccgaatact ccgtcaatta tccgtgaagg tgtgacagcc ttgactcctg
     4261 caaccggcct ctcggagaaa gatctggcga ttgtccaggc gatgttcgag gtcatcggga
     4321 ctgtcgtggt cgtccctgag cggtctatgg atgccgtcac cggactgagt gggagcggac
     4381 ctgcgtatgt ctatattatg atcgaagcgt tagccgacgg cggagtcaag atggggcttc
     4441 caagagagac ggcacaactg ctcgccgcac aaacggtggc gggagccgca cgattggtgc
     4501 tggagtctca tgaacacccg ggagttctga aggatcgtgt agcatcgccg ggaggcacga
     4561 cgattgcggg cctttatgaa ttggaacggc ggtgtttccg tgcgacgatg atatccgcgg
     4621 tggaggcggc aagccggcgt tcagccgagc tgggcatggt ttcgtaagaa aggagtcatg
     4681 aggtgcaata ttgggtgaaa gtagtatttg tcgacaacca ggaattgatg gtcaaggatg
     4741 ccattcgcca tacgatcagc gatgacatgg aagtgctcga agtcgatacg cccaaagagg
     4801 tgtttattat ccc
//
LOCUS       DATDNW010000328         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1341507, whole genome shotgun sequence.
ACCESSION   DATDNW010000328 DATDNW010000000
VERSION     DATDNW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341507"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VI359_07880"
     CDS             complement(<1..298)
                     /locus_tag="VI359_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6974209.1"
                     /translation="MKVLVAIDGSKYGRWGLEWIAHLPLAAPPQVTALHVIDVAALRA
                     PFMVQPVIVGNERYIREEIQRLEERGKKTVAEARTVMTSLKLKGKVVKEQNAVAV"
     gene            complement(350..1597)
                     /gene="pfp"
                     /locus_tag="VI359_07885"
     CDS             complement(350..1597)
                     /gene="pfp"
                     /locus_tag="VI359_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEY6974210.1"
                     /translation="MNQPRTTVGILVGGGPAPGINSVIGAATIRSLLAGCEVFGFING
                     FEWLMQGNTSHVQPLSFEEVSRLHFRGGSLLGTSRANPTKKPEHLNQVVSALTGLGVT
                     RLITIGGDDTAFSALKVEEKAAGRLQVVHVPKTIDNDLDLPYSIPTFGFQTARHIGVD
                     IVKNLMVDAQTTTRWYFVVTMGRKAGHLALGIGKAAGATLTLIPEEFPQRPVQLAQLV
                     DTLAAAMIKRASEGRFDGVAILAEGLVEVLDPQGLGGLDHVERDQHGHIRLSEVDIGE
                     VLKREVAKVLGGFNLKTTIVAKNIGYELRCADPIPFDMEYTRDLGYCAAQYLLDGGTA
                     AMISIQQGRFTPIPFKEMLDPATGRAKVRMVDVESESYQVARQYMIRLSQDDLTDEPT
                     LGRCASVVGLSPEAFRTRFGSVV"
     gene            1893..>2343
                     /locus_tag="VI359_07890"
     CDS             1893..>2343
                     /locus_tag="VI359_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY6974211.1"
                     /translation="MTISAQFPQEQSDEGEFIRQADAFREWVTADGRSGFPAAKGRYH
                     LYVSWACPWAHRTIIVRKLKRLEDVIGMTVVDPIRDERGWAFREGSGHSLDPINGFRF
                     LSEAYRATDSHYRGRVTVPVLWDTVRKRIATNSDDDLMRMFNGEFNRF"
BASE COUNT          448 a          746 c          652 g          497 t
ORIGIN      
        1 tcaccgccac ggcgttctgc tctttgacga cttttccctt gagtttgagc gaggtcatca
       61 cggtcctggc ctccgccacg gtttttttgc ctcgctcctc gagccgctga atctcctcgc
      121 ggatgtagcg ttcgttgccg acgatcaccg gctgcaccat gaacggggct cgcagcgctg
      181 cgacgtcgat gacatgcagc gcggtgacct gaggcggtgc cgcaagcggc aggtgcgcga
      241 tccattcgag cccccaacgc ccatatttcg atccatcgat cgcaacaagg actttcatcg
      301 tacgtatcct ttcgtcaaca caccgtccgc tctcgtgaca ttcgtcgact cacacgacag
      361 aaccgaatcg agtccgaaat gcttctgggg ataagcccac caccgaggcg catcgtccca
      421 gcgtcggctc atcggtcagg tcgtcctgag acaggcgaat catatactgg cgcgcaacct
      481 ggtaggactc cgactcgacg tctaccatcc ggaccttcgc ccggccggtt gccggatcca
      541 acatctcttt gaaagggatc ggcgtgaagc gaccctgttg aatcgagatc atcgccgcgg
      601 tgccgccgtc aagcagatac tgtgccgcgc agtacccgag atcgcgcgtg tattccatat
      661 cgaacgggat cggatcggcg cagcgcaact cgtatccgat gttcttggcc acgatggtgg
      721 tcttgaggtt gaagcccccc agcactttcg cgacctcccg tttgaggact tctccaatat
      781 cgacttccga gagccgaatg tgaccgtgct ggtcccgctc cacgtggtcc agcccgccca
      841 ggccctgagg atccagcact tcgaccagcc cttcggccaa tatggcgacc ccgtcgaacc
      901 gcccctcgct cgctcgtttg atcatcgccg ccgccagcgt atcgactaat tgcgccagct
      961 gcacaggacg ctggggaaat tcctcgggaa tgagagtcag cgtcgcaccg gcggctttcc
     1021 cgatccccag tgcgaggtgg ccggctttcc gtcccatggt cacgacgaag taccagcggg
     1081 tggtcgtctg ggcatccacc atcagattct tcacgatgtc cacgccgata tggcgggccg
     1141 tttgaaaccc gaaggtcgga atactgtacg gaagatccag atcgttgtcg atcgtcttcg
     1201 ggacatgcac gacctgaaga cgaccggccg ctttctcttc cactttcagc gccgaaaacg
     1261 ccgtgtcgtc cccgccgatg gtgatgagcc tggtgacacc gagcccggtc aatgccgaca
     1321 caacctggtt gagatgctct ggtttcttgg tgggatttgc ccgcgaggtc ccgagcaatg
     1381 acccgccgcg gaaatggagg cgactgactt cctcgaacga gaggggctgc acatgactcg
     1441 tgttcccctg catcagccat tcgaacccat tgatgaagcc gaagacctca cagccggcca
     1501 gaaggctccg aatcgtggcg gctccgatga cgctgttgat gccgggggca ggaccgccac
     1561 cgacgaggat gccaacggtc gtgcgcggct ggttcacgat tccctccttc tttcgactcg
     1621 tatcatttcg cctgtctcag gcacaattca ttggattcac gcacgaggat tgttccctcc
     1681 agaatcttct cttgcttata aaggaggcag gtctcttcgt taactctcgt cgatgacacg
     1741 gctgccctta tcttacccgg taccatcgcc tacacgccaa ccgaaaactt cgatcgctgg
     1801 tgtttgcccg gcgcctggcc ggatgccgcc ggctggatac gacggtgcac ttcataggca
     1861 gaggccgcgg cgaaagagta agatgcgatt taatgacgat ctcggcgcaa tttccccagg
     1921 aacagtctga cgaaggtgaa ttcatccgac aggcggatgc ctttcgcgaa tgggtcaccg
     1981 cggacggccg ttccggtttt cccgccgcca agggccgtta ccacctctat gtgtcatggg
     2041 cctgcccatg ggcccatcgc acgatcatcg tgcggaagtt gaaacgactt gaggatgtca
     2101 tcggcatgac cgtcgtggac ccgatccgcg atgaacgagg ctgggccttc cgtgaaggct
     2161 ccggccattc cctcgatccc atcaatggat tccgattcct cagcgaggcg taccgtgcga
     2221 ccgactcgca ttatcggggg cgcgtgaccg taccggtgtt gtgggatacc gtccgcaaac
     2281 gcatcgcgac gaattccgac gacgacctca tgcgcatgtt caacggcgaa ttcaaccgtt
     2341 ttt
//
LOCUS       DATDNW010000329         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_592522, whole genome shotgun sequence.
ACCESSION   DATDNW010000329 DATDNW010000000
VERSION     DATDNW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592522"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1134
                     /locus_tag="VI359_07895"
     CDS             238..1134
                     /locus_tag="VI359_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974212.1"
                     /translation="MRNKIPCSAERLSLLDPPMIQSVVDRGAFQIGSQYVAENRVRIV
                     EADESQISSAVIGSSGLYEQTIRLKDGHLVSKCSCALPEEPMCRHCIAVLLEYHRWAQ
                     PRTSRKPKPAPPVQTAASPSNGNGTVSAGTSSSVDLKLSEVMAFIEWLQPAMKAVERG
                     QPMPDSSKLSGDVAAWAHAMRNLDERRREGEEIQLSLESEIRDREAYVGRLTNQLQAS
                     MGEVKTAQVAHQEAMQELATYKSALARVADLATEVGGYDNQLKSIAGDMLSKGTQLEK
                     LATSFREVAAALRTLTKSGPSQ"
     gene            1249..1440
                     /locus_tag="VI359_07900"
     CDS             1249..1440
                     /locus_tag="VI359_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974213.1"
                     /translation="MRKMVARIGTVAGSILLVGASQVLANEPKGEGAPDYSGISYMYY
                     TLIGIILAYGVYDTFLKKS"
     gene            complement(1535..2104)
                     /locus_tag="VI359_07905"
     CDS             complement(1535..2104)
                     /locus_tag="VI359_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY6974214.1"
                     /translation="MTPSSVEQRRIVAFGNSLTAGLGVGPSESYPAQLQQRLDDLGYH
                     YRVVNAGVSGETTAGGVRRVAWVLKSRPVIVILELGANDGLRGLNPDQTRANLEHIIQ
                     QLQQARVTVVLAGMKLPPNYGVEYTRAFESIYPALARRYHLELIPFFLDGVAGSTKLN
                     QADGIHPTAEGYRVIVGKILETVQPLLRK"
     gene            2298..3002
                     /locus_tag="VI359_07910"
     CDS             2298..3002
                     /locus_tag="VI359_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974215.1"
                     /translation="MIAVQGLSMRLAAGGRAITILDQIDLVIPSKQMVAIVGPSGSGK
                     STLLGLMAGLDRPTSGSVVLDEVDITTLSESALARYRRKTIGYIFQAFHLIPTLTALE
                     NVMVPLELAGVSSAKARAIDLLDVVGLHDRLHHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSKTGQHIIELLLDLHRDYGTTLVLVTHERDVASLMQRVIILRDGRIE
                     SDEETDPTVHTEGSSS"
BASE COUNT          693 a          874 c          919 g          687 t
ORIGIN      
        1 aagccgccgt tttgtgacgg tacccactca agaatttaag gccgatggac gacgatttac
       61 gacatcctga agaggaaact cctccatggt tctgctcgcc gctgtcttag cctctggacc
      121 gcctctccgc caggggtgca actgccggaa ccaggcgaac gcttgacagt tgaatcagtc
      181 cgttccagac taccccggaa tatcacacgg gtcatctcga acttgggact tcaacggatg
      241 cgaaacaaga tcccctgctc agcggagcga ctcagtcttc tcgacccccc tatgattcag
      301 tcggttgtgg atcggggtgc ctttcaaatc gggagccagt atgttgcgga aaatcgggtc
      361 cgcatcgtcg aagcggatga atcccagatt tcctcggctg taatcggcag ctcaggattg
      421 tacgaacaaa cgatcaggct gaaggatggg cacctggtgt cgaagtgcag ttgcgctctg
      481 ccggaagaac ccatgtgccg ccattgcatc gctgtattgc tggaatatca tcgatgggct
      541 caaccacgca caagtcggaa accaaagccg gctccccccg tacagacggc ggcaagccct
      601 tcgaacggga acggaactgt gtcggctggg acttcttcct ccgttgattt gaaactcagc
      661 gaggtcatgg ccttcatcga gtggctgcaa ccagccatga aggccgtgga acgaggtcag
      721 cccatgccgg attcctccaa gctgagtggc gatgtggctg cctgggcgca tgccatgagg
      781 aatcttgacg aacgccgccg cgaaggagag gaaattcagt tgagcttgga gtcagaaatt
      841 cgagaccgag aagcctacgt agggcggctg accaaccaac ttcaggcatc catgggagag
      901 gtcaagacgg ctcaagtcgc ccaccaagag gcgatgcaag aacttgcaac ctataagagc
      961 gcactagcaa gagtggcaga tcttgcgacc gaagtcggtg ggtacgacaa ccagctcaag
     1021 tcgattgcag gagacatgct ctcaaaagga actcagctcg agaagctggc tacatcgttc
     1081 agagaagtgg ctgcagccct gaggaccctc acaaagtccg gcccctctca ataacgcacc
     1141 ccttgcggcc accgccggtc cgtttcgttc gaaccctctt gcgtctcaaa agccaagttc
     1201 agtacaatgc cgcccacttt catgttgtct gattcgagga ggataggtat gagaaagatg
     1261 gttgcgcgaa tcggtacggt cgcgggctca atactgctgg ttggcgcctc ccaggtgctg
     1321 gccaacgaac cgaaaggcga aggtgcgccg gattattccg ggatcagcta catgtattac
     1381 acgctgatcg gaatcattct tgcctacggt gtgtacgaca cgttcctgaa aaagagctaa
     1441 caactctttc cgagatgatc ggacaccagg gctcttggtt cgctcatgag tcctggtgtc
     1501 cacctgttct gatccacatc atcgagaacc cctctcactt ccttaggagg ggctgcaccg
     1561 tttccagtat cttaccaaca ataacgcgat agccttccgc ggtggggtgt attccgtcgg
     1621 cttgattcag tttggtcgat ccggctacgc cgtcgagaaa aaacggaatc agctcgagat
     1681 gatatcgtcg ggccaaagcg ggatagatcg attcaaaggc cctggtgtac tcaacaccgt
     1741 agttaggcgg cagcttcatg ccggccagca caacagtgac tctggcctgc tggagctgtt
     1801 gaataatgtg ttccagattc gcccgtgttt ggtcaggatt gaggccacgg agcccatcgt
     1861 tggctccgag ttccagaatg acgatgacgg gccggctctt gagcacccag gccacacgcc
     1921 ggactcctcc cgccgtggtt tctccgctga ctcccgcgtt gaccacgcga tagtgatagc
     1981 ctaagtcgtc aagtcgttgt tgcaactgcg cgggataaga ttctgaaggt ccgactccca
     2041 gaccggctgt caggctgttg ccaaacgcga caattcgccg ctgctcaaca gacgacggcg
     2101 tcaaaccgtc ggtcgaggac agtgttgagg cttgagggcc aggtgataac gtaaccgcgg
     2161 gcattggcgc cagtatcacg agggacgacc acaggctcat caggccggct aggaacttgt
     2221 gggaaggtac ggggtctgtc ataggagtac agtataatac ggtcgggttc gctcgagtac
     2281 cgtgaggagt tcttgccatg attgccgtgc aggggttgtc gatgcggttg gccgccggtg
     2341 ggcgggcgat cacgattctc gatcagattg atctcgttat cccatcgaaa caaatggtag
     2401 cgattgtggg gccctccggc agcggaaagt ccaccctttt gggtctgatg gcagggctag
     2461 accgtccgac ttcaggctcc gtcgtgctcg acgaagtcga catcacaacc ttgtctgaga
     2521 gcgccctcgc tcgataccgg cgaaagacga ttgggtatat ttttcaagcg ttccatctta
     2581 ttccaaccct gaccgcgctt gagaatgtca tggtcccgct ggagttggcc ggagtatctt
     2641 cagcgaaagc gcgtgcgatc gacctcttgg atgtggtcgg tttacacgac cgtcttcacc
     2701 attacccggt gcaattatcc ggaggcgagc aacagcgggt tgcagtagcg agagcgttcg
     2761 cctgccgccc tccgatcctg ctggccgacg agccgaccgg taaccttgat tcaaagacag
     2821 gccagcacat aatcgagctc ctgctcgacc tgcaccgcga ctacggcacg acgctggttt
     2881 tggtcacgca tgagcgagac gttgcctcac ttatgcagcg ggtgatcata ttgcgggatg
     2941 gtcgcatcga atcggacgag gaaaccgacc caacagtcca cacggaaggg tcctcctcgt
     3001 gaatatgttt ctgttcgcca tggcctggcg agaaacgagg ggagcgtggc ggcattttct
     3061 gtattttttc gtctgtatcg ccgttggtgt cggtgcgttg gtcggtgtgt cactattcgc
     3121 caccaatgtg gaacgagcta tcattcggga agcgcgtggg ttgctcggcg gtg
//
LOCUS       DATDNW010000330         4212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_178736, whole genome shotgun sequence.
ACCESSION   DATDNW010000330 DATDNW010000000
VERSION     DATDNW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178736"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VI359_07915"
     CDS             complement(<1..553)
                     /locus_tag="VI359_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HEY6974216.1"
                     /translation="MDSKEQLIDAPLQASSPFELRMNLGRSLSDSDVRTALATGDMGF
                     LHSFTTGSTVDGPGVRVVAWTTGCQFRCLYCHNPDTWNLTNGIPVTIGRATEELRKYR
                     VGLKVMSGGFTLSGGEPLMQDRFAVKLLQAAQAMGIHTALDTNGSLGNRLGDDELKTI
                     DLVLLDIKSWDNERHRQLTGMEVG"
     gene            complement(560..2797)
                     /gene="pflB"
                     /locus_tag="VI359_07920"
     CDS             complement(560..2797)
                     /gene="pflB"
                     /locus_tag="VI359_07920"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEY6974217.1"
                     /translation="MTTDEVGSPWQGFKPGLWQREINVRDFIQENYDPYEGDGGFLAP
                     ATDRTRKLWGRLNSLFAEERKKGVLEISQIPASITAHGPGYIDRNNEVIVGLQTDAPL
                     KRAIMPNGGLRMVLNALKAYGYEPDPHVVEAFTKYRKTHNDAVFDAYTADVRRCRSSH
                     ILTGLPDAYGRGRIIGDYRRVALYGVARLIQKKQEEKQSLDSAMSTDEIIRDREELSE
                     QIRALKELQLMASSYGVDVSKPARNAGEAVQWLYFGYLAAVKEQNGAAMSLGRTSSFL
                     DIYFQRDMAAGAMTEKQAQEIIDDFVIKLRIVRFLRTPEYDDLFAGDPTWVTESIGGM
                     GDDGRTLVTRTSYRFLQTLYNLGPAPEPNLTIWYSPRLPEGFRNFAAKVAIDTSSIQF
                     ESDEIMRRAWGDDGAIACCVSPMLVGKQMQFFGARANLAKCLLYAINGGRDEMTGEQI
                     GPQNGAVRGEYLDFDVVIEKFEAMMEWLAHVYVNAMNIIHYMHDKYAYERLEMALHDY
                     APLRTMAFGMAGLSVVADSLSAIRYTKVRVIRDATGLVTDYQTEGEFPQFGNNDNRVD
                     QLASWVVSTFMRQLRKYPTYRNAIHTQSILTITSNVVYGKSTGNTPDGRRQGEPFAPG
                     ANPMHGRDKHGIHASAASVAKIPYRDAADGISLTSTLIPESLGRVAEDRITNLRNILD
                     AFFSATGFHMNVNVLNREMLLDAMDHPEKYPHLTIRVSGYAVNFVRLTREQQMDVINR
                     TFHGN"
     gene            complement(2856..4043)
                     /locus_tag="VI359_07925"
     CDS             complement(2856..4043)
                     /locus_tag="VI359_07925"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY6974218.1"
                     /translation="MDDYTLILNAGSSSLKFCVYRRPATQKSWGLEARGQIEGIGTSP
                     RFFAKGSQGTILDDTALDGWVCDGRAALDSLASWLRSRYVSARLLGVGHRVVHGGARY
                     DRPIVVNRQVLSELYELIPLAPLHQPHNLAAIDTIFERLPDVPQVACFDTSFHQGHCQ
                     VAKLVPLPKEIRKSGLQRYGFHGLSYEYIASVLPEVAPEIAKGRVIVAHLGSGASLCA
                     LRGGKSVDSSLSFTALDGLCMGTRPGTLDPGVVLYLFQTLGLSPKEVESILYKKSGLL
                     GISGISNDMRDLLARGEPEARLAVDYFIYRAVKEIGALAAVLGGVDGLVFTAGIGENS
                     PEIRRRICEASSWLGVQLDEEANSNHSARISTRQSKVSTWVIPTNEELMIARHTGSLL
                     GLV"
BASE COUNT          912 a         1235 c         1134 g          931 t
ORIGIN      
        1 gaccaacttc cattccggtc agttgacgat ggcgttcgtt atcccagctt ttaatatcga
       61 gcagaaccag atcaattgtc ttcaactcgt catcgccgag acggtttccc aggctaccgt
      121 tagtgtccag cgccgtatgg attcccattg cttgcgcggc ctgcaacagc ttcacagcga
      181 accgatcctg cataagaggt tcgccgccgc tcaaggtgaa gccgcccgac attaccttca
      241 gtcccacccg atacttgcgc agctcctccg ttgccctccc aatagttact gggattccgt
      301 tggtcaggtt ccaagtgtct gggttgtgac agtacaggca tcggaactga cagccagtgg
      361 tccacgcaac cacgcggaca ccagggccat ccacggttga gccagtggtg aaagaatgaa
      421 gaaagcccat gtccccagtt gccagcgcgg tccttacgtc ggagtcggag aggctccgtc
      481 ccagattcat gcgcagttcg aaaggactgc ttgcttgcag tggagcatca atcaattgtt
      541 ccttgctgtc cataagccat tagttcccgt gaaaagtacg gttgatgacg tccatctgct
      601 gttcgcgagt cagccgcaca aaattcacgg cgtagccgga gacgcggatg gttaggtgcg
      661 ggtacttctc cggatggtcc atagcatcca agagcatctc gcgattcagc acattcacgt
      721 tcatgtgaaa cccggtagcg ctgaaaaaag catcgaggat gtttcttaga ttcgtgattc
      781 ggtcctctgc aaccctcccc agactttctg ggatcaacgt gctggtgaga gagatgccat
      841 cagcggcgtc gcgatagggg atcttcgcga ccgatgccgc cgaggcatgt atgccatgtt
      901 tgtcccgtcc gtgcatcgga ttggcgcctg gagcaaacgg ctcaccctga cgtcgtccgt
      961 ccggggtgtt gccggtgctc tttccgtaca caacatttga ggtgatggtg agaatggact
     1021 gtgtgtggat ggcattccgg taagtgggat attttctcag ctggcgcatg aatgtgctga
     1081 caacccagga ggcgagttgg tcaacgcgat tgtcgttgtt accgaactga ggaaactcgc
     1141 cctccgtctg atagtcggtc actagcccgg ttgcatcgcg gattacccga actttggtgt
     1201 aacgaatcgc cgacaaactg tcagcaacaa ccgacaggcc agccatgcca aaggccatgg
     1261 tgcgcaacgg agcgtagtcg tgcagagcca tctcaagacg ctcgtaggca tacttgtcgt
     1321 gcatgtagtg gatgatgttc atggcgttaa catagacgtg ggcgagccac tccatcatcg
     1381 cttcgaactt ttcgatcaca acgtcgaaat caagatactc accgcgaaca gcgccgtttt
     1441 gggggccaat ctgctcgcca gtcatttcgt ctcggccacc attgatggca tagagaaggc
     1501 atttggcgag attggctcga gccccgaaaa actgcatctg ctttcccacc agcatcggcg
     1561 agacacaaca agcaatcgcc ccatcgtcgc cccatgcgcg gcgcatgatt tcgtcacttt
     1621 cgaactgaat ggagctggtg tcgattgcca ctttggcagc gaagttccgg aacccctccg
     1681 gcagccgagg cgaataccat atcgtcagat ttggctcggg ggcggggccc aggttatata
     1741 acgtctgaag aaaacggtaa ctggtcctgg tgaccagcgt tcttccgtcg tctcccatgc
     1801 cgccaatcga ctctgtgacc caggtcggat caccagcgaa caggtcatcg tattctggag
     1861 tgcgtagaaa acgaacgatg cgcaacttga ttacgaagtc atcaatgatc tcctgcgcct
     1921 gtttctccgt catggcaccg gcagccatat cgcgctggaa gtagatgtca aggaaacttg
     1981 aggtcctccc cagcgacatg gcagcaccgt tctgttcctt caccgcagcg aggtagccaa
     2041 agtaaagcca ttgcaccgcc tcgcccgcgt tgcgagctgg cttcgagaca tcgacgccgt
     2101 agctggaagc catgagctgc agttccttaa gcgcgcggat ctgttcgctg agctcctccc
     2161 ggtcgcgaat gatttcatcc gtggacatgg ccgagtcaag gctctgcttt tcttcctgct
     2221 ttttctgaat cagccgcgcc actccgtaaa gagccacgcg gcggtagtca ccgataatac
     2281 ggcctcgtcc gtaggcatcc gggaggccag tcagaatatg agagctgcgg catcgtctga
     2341 catctgccgt gtaggcgtcg aagacggcat cgttatgagt cttacggtac ttggtgaacg
     2401 cttcgaccac gtgcggatca ggctcgtaac cgtaggcttt tagagcattc aaaaccatgc
     2461 ggaggccgcc atttggcatg atggcccgct tgagcggcgc gtccgtttgg agaccgacaa
     2521 tcacctcgtt gtttcggtca atgtatcctg gaccatgcgc tgtgatggag gctgggatct
     2581 gcgagatttc gagcacaccc ttcttgcgct cctccgcaaa aaggctattc aaccggcccc
     2641 aaagcttcct ggtgcgatcc gttgcagggg cgaggaatcc cccatcccct tcataagggt
     2701 cgtaattttc ctggatgaag tcgcgaacgt tgatttctct ttgccacaga ccaggtttga
     2761 atccctgcca cggagaccct acctcatcag tcgtcatctc gtacgaacga gcagtagcca
     2821 cttgcgatcc tccttatctc gaaaaaaaaa caaccttaga ccaacccaag caatgagccg
     2881 gtatggcgcg cgatcatgag ttcctcgttg gtcgggatca cccaggtgga gactttgctc
     2941 tgtcgagtgg aaattcgcgc actgtggttg ctgttagctt cttcgtccag ttgtacgccc
     3001 agccaggaag aggcctcgca gatacgacgg cgaatttctg gtgaattctc acctatacct
     3061 gccgtgaaca cgagcccgtc caccccgccc agtaccgcag ccagggcgcc gatttccttg
     3121 accgcgcggt atatgaagta atccaccgcc agccgggcct ccggttcgcc tcgggccagt
     3181 aggtcgcgca tgtcattgct gatcccagag atgcccagta gccccgattt tttgtagaga
     3241 atggactcga cttctttcgg agatagtccg agggtctgga aaagatacaa aaccacgcct
     3301 ggatccaacg tacccggcct ggtccccatg cagagtccat ccagcgctgt aaagctcaga
     3361 ctgctatcca cgctcttccc gcctcttaag gcgcacaaac tcgcgccact tccaagatga
     3421 gcaacgatga cgcgcccctt ggcaatttcc ggagccacct cgggcaacac cgaagcgatg
     3481 tattcataag acaggccgtg gaagccatat cgctgcaagc ccgatttacg gatctctttt
     3541 ggtagcggca ccagcttcgc aacctgacag tggccttgat ggaagctggt gtcgaagcag
     3601 gcaacttgcg gcacatcagg cagccgctcg aagatagtgt caatggccgc cagattgtgc
     3661 ggctgatgca ggggagccag gggaatcagc tcgtatagtt ctgacaaaac ttgccggttt
     3721 acaacgattg gccggtcgta ccgcgccccg ccatgaacca cgcggtggcc aacgcccaac
     3781 aggcgtgcac taacatacct ggagcgaagc cacgatgcca gcgaatccag cgcagcgcgg
     3841 ccatcgcaca cccacccatc caatgcggtg tcgtcgagta ttgttccttg agagcctttc
     3901 gcaaaaaatc gtggcgaggt tccgatgccc tcaatctggc cgcgtgcctc cagcccccag
     3961 cttttctgcg tggctggccg ccgatacaca caaaacttca ggctagacga gcccgcgttc
     4021 aatatgagcg tgtaatcatc cataggagca tctatccctt ttttcggcga gcgtgagctg
     4081 ccagcatagc tactgcacaa cgggcgattc gcgaccgcac gctatcggcc cgtctggtca
     4141 ggattatggg aaccctcgct cccagtacca gcccggcact gtccgcatta gcgaggaagc
     4201 tcagctgctt gg
//
LOCUS       DATDNW010000331         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1206504, whole genome shotgun sequence.
ACCESSION   DATDNW010000331 DATDNW010000000
VERSION     DATDNW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206504"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VI359_07930"
     CDS             <1..693
                     /locus_tag="VI359_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974219.1"
                     /translation="VTPSPGIISLARSVISVLDKFMFTFVSPYRSEIIRIGWICGVIA
                     GQSLLFCLMNETHADVRMEVQAPASHIGAAMALLATLQDSDVLPPEGTPAANRIVKSV
                     IQCQSLFMKSSDPDVRAFFDSALQRKLNGHATETIRRFRNEGWTSEVLEALSESFVAL
                     SDRQRVQMAEGFRAYNLTPSDFLQLSELFLKARTTLKQRGQDIHQIYARRRQEMPGRP
                     FPHSPESPTSPS"
     gene            707..943
                     /locus_tag="VI359_07935"
     CDS             707..943
                     /locus_tag="VI359_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY6974220.1"
                     /translation="MATKAYILIKVKAGRTKDVLQSLKRISGVEQAHSCFGQPDIFLF
                     ISTNDERALSEVVISKIHSIEGVEETDTHIVAES"
     gene            complement(940..1929)
                     /locus_tag="VI359_07940"
     CDS             complement(940..1929)
                     /locus_tag="VI359_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY6974221.1"
                     /translation="MTASSSLGIGLIGVGRHGSRYVRHLLHDMPDTNLVAICRRRIEE
                     GIGGLPLSSIPVYGDYRALIADPLVRAVIVVTPPALCHEVCLEAIRVGKPLLIEKPLA
                     PTAQQARAMVRAANQAGVLLMTAHTLRFEPVIEKLKSTLPQLGTLRSATMTSHIEVTS
                     SIMPMTNGFGRRGALLEIGIHLLDAVRFLTSDEVSAVRCHMDLAPPAGPESRATVQLT
                     TARGIECHIEVARVTAGRIAQMEWIGTEGRVSADWFRHRLMKQEGAGSTEEWTVEPRP
                     TILSVLRAFVHAIRTNSCPPVTGEDGQRAVEIAEACYRSAELGGQLVKVESDY"
     gene            2098..>2322
                     /locus_tag="VI359_07945"
     CDS             2098..>2322
                     /locus_tag="VI359_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974222.1"
                     /translation="MTSALAPFTLEQIGTDTARFPSGVAIPTPTDAAVDPYIKTRMSK
                     DVQVEAIQFWPQEKGTYPGLVLIHEWWGLNS"
BASE COUNT          532 a          637 c          620 g          533 t
ORIGIN      
        1 gtgactccct ctcccggtat aatttcactc gctcgatccg tcatctccgt tctcgacaaa
       61 tttatgttca cctttgtctc cccttatcga tccgaaatca tacgaattgg atggatctgc
      121 ggcgtcatcg ctggccagag ccttttattc tgtttgatga atgagaccca cgctgatgta
      181 cgaatggagg ttcaggctcc ggccagccat atcggagctg cgatggcgct cttggcgaca
      241 ttgcaggaca gcgacgtgct tccgccggag ggcacgcctg ctgcgaaccg catcgtgaaa
      301 tcggttattc aatgtcagtc tcttttcatg aaaagttcgg atccagacgt tcgagcattc
      361 tttgatagcg cgctgcagcg caaattgaat ggccacgcca cggagaccat tcgacggttc
      421 cgaaatgaag gctggacctc agaggtactc gaagcactta gtgagtcatt tgtagccctg
      481 tcagaccgcc agcgcgttca gatggcggaa ggattccgtg cttacaacct gaccccgagt
      541 gattttctgc agttgagtga gttattcctc aaggccagaa cgactctgaa acaacgaggg
      601 caggatattc atcagattta tgcacgtcga cgacaagaga tgccgggccg accatttcct
      661 cacagcccgg aatcaccgac ctcaccttca tgaaaggagc agcacgatgg caacaaaggc
      721 ctacatactg atcaaggtca aggcaggaag gacgaaggac gtacttcagt cgctcaagcg
      781 catcagtgga gtcgagcagg ctcattcctg ttttggacag ccggatattt ttctctttat
      841 cagcacgaat gacgaacggg ctctgtcgga ggttgtgatt tccaaaatac actcgataga
      901 aggcgtggag gaaaccgata cccatattgt ggccgaatct tagtaatcag attcgacctt
      961 gacgagttgg cctccaagtt ccgctgagcg ataacaggcc tcggcgattt ctactgctcg
     1021 ttgcccatct tctcctgtaa caggcggaca agaattcgta cgaatggcat gaacaaacgc
     1081 gcggagcact gagaggattg tcggtcgcgg ctccacggtc cattcctctg tgcttccggc
     1141 tccttcctgt ttcatgagtc ggtgtcgaaa ccaatcggca ctcacccgtc cttcggtccc
     1201 aatccactcc atctgtgcga tgcgtcctgc tgtgacccgc gcgacttcaa tatgacactc
     1261 gattccccgt gcggtcgtga gttgtacggt cgctctggat tccggccccg cgggtggcgc
     1321 aaggtccata tggcaacgaa cagcggatac ctcgtcgctg gttaaaaacc gcaccgcatc
     1381 tagcagatga ataccgatct cgagtagtgc tcctcgtcga ccgaacccat ttgtcattgg
     1441 catgatgctc gatgtcactt cgatgtgact ggtcatcgtc gcagaacgaa gcgtcccgag
     1501 ctgcggaagc gtgctcttga gcttttcgat gactggttca aaccgcaggg tatgggctgt
     1561 catgaggaga acacccgctt ggttggctgc tctgaccatg gcccgagcct gctgtgcggt
     1621 gggagccagg ggcttctcga tcaacaaggg cttaccgacc cgaatcgctt caaggcaaac
     1681 ttcatgacaa agcgcaggcg gagtgacgac gatgacggcg cggaccagcg gatcggcgat
     1741 caaggcacgg tagtcaccgt agactggaat tgaagagagc gggagtcctc cgataccttc
     1801 ttcaattcgc cttcgacaga tggcaacaag gttcgtatcc ggcatgtcat gcaggagatg
     1861 ccgcacatag cgactgccat gccgtcctac accgatcaat ccgataccaa gtgaagaaga
     1921 tgctgtcata tggagcctct cctatcgaga ctctatgagg aaggatcaat agggtcaatc
     1981 gaatcttgtt ctccgtcggc caattgacaa cgcgaaggtg gtctccctat agttattgcg
     2041 ttgcacgatg tcttatggtc tggtagacgg cgacatcttg ggaaagcgat tcagattatg
     2101 acctcggcgc ttgcgccctt cacgcttgaa caaatcggca ccgacaccgc gcggtttccc
     2161 agtggggtag ccattccaac tcccacagac gccgccgtcg atccctacat caaaacccgc
     2221 atgtcgaaag acgtgcaggt ggaagccatt cagttttggc cacaggagaa ggggacgtac
     2281 ccaggcctcg tcctgattca cgaatggtgg ggattgaatt cc
//
LOCUS       DATDNW010000332         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1146609, whole genome shotgun sequence.
ACCESSION   DATDNW010000332 DATDNW010000000
VERSION     DATDNW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146609"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VI359_07950"
     CDS             <1..387
                     /locus_tag="VI359_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY6974223.1"
                     /translation="FDILVHLSPTIVDERTRELRIKLIQEEFEELKEALTHGDLESIS
                     KEIADLLYVVYGTAVSYGIDMDPVFREVHRSNMSKVGGYKREDGKWVKPATYSPACIE
                     PILAEQSGASPVRLESEVVRQEVPSS"
     gene            387..899
                     /locus_tag="VI359_07955"
     CDS             387..899
                     /locus_tag="VI359_07955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974224.1"
                     /translation="MKELHCPNCGTPFVRATSREGPVERLLSCINIFPFRCQLCTNQF
                     RAFWSGARGNTQAFDKRQYKRLPTSIEAQILTDSNQRGTNRITEISMGGCTLRTTAEF
                     PRGTFLELTLKPTSGEEPITIESGMVSSVRPSSLGISFLEMAPRDKQRLSQIVLGLLV
                     SLSPLPASGM"
     gene            complement(907..1761)
                     /gene="panC"
                     /locus_tag="VI359_07960"
     CDS             complement(907..1761)
                     /gene="panC"
                     /locus_tag="VI359_07960"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY6974225.1"
                     /translation="MKIIRSPQAMTAWSEGLRREGVTIGFVPTMGALHDGHRALIRAA
                     RLQCDALVVSIFVNPTQFGPREDFTNYPRPIAKDHALCRTEGVDVCFEPPASVMYPEG
                     FQTIVMVPAIARRWEGESRPHHFAGVATVVTKLLGIVRPHHVVFGQKDYQQAAVVRQL
                     IADLNLGVTFDVRATIREPDGLAMSSRNVYLSTEERRIAPTFYRALQAGRQAIADGAT
                     DAASVQEAMRKVIGGEPTIQIDYLAICDSTTLEPLATIGREAVVLGAIRIGSVRLIDN
                     VVVKRPAR"
     gene            complement(1758..>2280)
                     /gene="folK"
                     /locus_tag="VI359_07965"
     CDS             complement(1758..>2280)
                     /gene="folK"
                     /locus_tag="VI359_07965"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY6974226.1"
                     /translation="NVGDRVDFCDRAVTLLSLLPHSQVRGVSLLYETEPVYDHAQPGD
                     GWFLNGVVELETDVTPRSLLTVLREIERSLGRDDDNRLGPRTIDLDILFYGQRIIDEP
                     ELVVPHPRLHQRRFVLMPLSELDPLWVHPTRQRTVSQMLAEVQDQSEVRLLFPQPSTH
                     YGSRPTCSPPPGS"
BASE COUNT          474 a          651 c          675 g          480 t
ORIGIN      
        1 ttcgacattc tggtgcacct cagccccacc attgtcgatg agcggacgcg agagctgcga
       61 atcaaactga ttcaggagga gttcgaagag ctgaaagagg cgctcaccca cggcgacctt
      121 gagtcgatct cgaaagaaat cgccgatctt ttgtatgtcg tttacggaac agccgtgtct
      181 tacggcatcg atatggatcc tgtgtttcga gaggtccatc ggtccaatat gagtaaggtg
      241 gggggctaca agcgggaaga tggcaaatgg gtgaaaccgg cgacctactc accggcatgt
      301 attgagccga ttttggctga gcaaagcgga gcgtcacccg tcaggcttga atccgaagtt
      361 gttcgccaag aggtcccttc atcttgatga aggaactgca ctgtccgaat tgcggaacac
      421 cgtttgtccg tgccacctct cgagaggggc cggtcgagcg tctcctcagt tgtatcaata
      481 tcttcccctt ccggtgccag ctctgtacca atcaattccg cgcgttttgg tccggtgccc
      541 gcgggaacac acaagcgttc gataagcggc aatacaaacg tctccctact tccatcgagg
      601 cgcaaatcct gacagacagc aatcagcgtg gcacaaatcg gatcacggag atctcgatgg
      661 ggggctgcac gctgcgaacg acggccgaat ttccacgagg cacgtttctc gagctgacgt
      721 tgaagccgac gtcgggagaa gaaccgatta ccatcgaatc ggggatggtg tcctcggtcc
      781 gcccctcatc tctggggatt tcctttctcg agatggcgcc tcgcgataaa caacggctca
      841 gccaaattgt gctcggcttg ctcgtcagcc tcagccccct tccagccagt ggcatgtaag
      901 ccctcgtcat cgcgcgggcc gtttgaccac cacattgtcg attaatcgta ctgagccgat
      961 ccggatggcc ccgagcacga cggcttcgcg gccgattgtc gcgagcggtt caagcgttgt
     1021 tgaatcgcag atggccagat agtcgatctg aatggtgggc tctcctccga tcaccttccg
     1081 catggcctcc tgcaccgatg ccgcgtctgt cgctccatct gcaatcgcct gtcgaccggc
     1141 ttgcaaggcc cggtagaagg tcggggcgat ccgccgttct tccgtcgaga ggtagacgtt
     1201 gcgagaactc atggccaagc catcaggttc tctgatggtt gcgcgaacgt cgaaggtgac
     1261 gccaaggttc aagtccgcga tcagctgtcg aacgaccgct gcctgttgat agtctttttg
     1321 accgaagacg acatgatgcg gacgcacgat cccgagcaac tttgtgacga ctgtcgccac
     1381 ccctgcgaag tgatgtggcc gagactctcc ttcccagcgt cgagcgatgg cgggaaccat
     1441 cacgatagtc tgaaaccctt ctggatacat gactgatgct ggtggctcga agcagacgtc
     1501 gaccccttcc gtcctgcaca gcgcatgatc cttcgcgatg ggacgagggt agttggtgaa
     1561 atcttctcga gggccgaact gggtcggatt tacgaatatg ctcacgacca gtgcgtcgca
     1621 ttgcaaccgg gccgcgcgga tcaacgcacg gtggccgtcg tgcagcgccc ccatcgtcgg
     1681 aacgaatccg atggtcacgc cttcacggcg gagtccctcg ctccatgcgg tcatggcctg
     1741 tggggagcgg atgattttca tgaccctgga gggggactgc acgtagggcg cgacccgtaa
     1801 tgggtcgagg gctggggaaa cagcaggcgg acctccgatt gatcctgcac ctcggcgagc
     1861 atctgactga ctgtacgctg acgtgtagga tggacccaca gcggatcgag ttcgctcaaa
     1921 ggcatgagaa cgaatcggcg ttgatggagt cgagggtgcg ggacgacgag ttcaggctcg
     1981 tcgatgattc gctgtccgta gaataggatg tccagatcaa tggttctggg gcctaatcgg
     2041 ttatcatcat cccgcccgag cgagcgctca atctcacgga gcactgtcaa gaggctgcgc
     2101 ggggtgacgt cagtttccag ctcgacgacc ccgttaagga accagccgtc tcccggctgg
     2161 gcatgatcgt aaaccggctc cgtttcatag agcagcgaga ctcctctcac ttgagaatgc
     2221 gggaggagac tcaatagggt taccgctcgg tcgcagaaat cgacccgatc gccgacgttc
//
LOCUS       DATDNW010000333         6905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_517656, whole genome shotgun sequence.
ACCESSION   DATDNW010000333 DATDNW010000000
VERSION     DATDNW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517656"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VI359_07970"
     CDS             complement(<1..1226)
                     /locus_tag="VI359_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY6974227.1"
                     /translation="MIDILILLGLIVLSAVISTAEIGFFAVNETKLRAIAQNGSKRAK
                     MALQLRSDPQKLLSTILVGDRLIATATPMYATFLTLNMFGEERTFFSDTVAVAVGLLT
                     FVLLVTVDVIPKTLAAKFAVPVTLNMAYPVYGVQLLLKPILAVLVPLIYKLTGGKGLT
                     LPLVTEEELKIMLDEGGRAGAIEFEKVKMIKNVFQLKDITAEDAMTPRLYVFSLDGNL
                     HLKEAQELLYNSKYSRVPVYDGTLDNITGILYKTRALTELSKGRFDVKLKDIANPALF
                     VPAGKTADDLMKQFQQDKRHMAVVVNEFGGVMGIVTLEDLLEEVVGEIMDETDITEEL
                     IKRLGKNQILVHGRTEVHKINEFLKVDLGDEAVTISGLIQEQLGRIPKVGDELKIGNC
                     RILIHEADPRSIRSVQ"
     gene            complement(1499..2179)
                     /locus_tag="VI359_07975"
     CDS             complement(1499..2179)
                     /locus_tag="VI359_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY6974228.1"
                     /translation="MWKGLAVVWSRPLVIALLVGTSYLLVSCGGAAPRPTYFPGYPVG
                     YVERGVASWYGPGFHGNKTANGERYDMYKLTAAHRTLPLGSVAVVRSLTTGRHVTVRI
                     NDRGPFARGRIVDLSLAGAHALGMTGAGTDQIELRVVGYQGRTADMGVLRIQVGSFAD
                     HQNAVNLLERLKTNYKGGRIVTIELPEGRRYRVQVGEFMTEAQAQTAASRLESSLDIQ
                     PFVVRDDT"
     gene            complement(2182..2592)
                     /locus_tag="VI359_07980"
     CDS             complement(2182..2592)
                     /locus_tag="VI359_07980"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974229.1"
                     /translation="MPTTTQLLISEQSKPGVIAQVASVLGEAGVNIKAFSAPEVTGTG
                     KLRILVADLDGARAALKAAKGRFSEETALLLSLENKPGALKQVADLLTKARINIKCGY
                     CTPSREGKRAIVVLTVSNTEKALNVLRDQSLDEF"
     gene            complement(2860..3267)
                     /locus_tag="VI359_07985"
     CDS             complement(2860..3267)
                     /locus_tag="VI359_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974230.1"
                     /translation="MSTTFREQGIETLHDQVVSLVAQRWAQAFHCRVTIKTSLEQNPW
                     ADSNQQCDIVGWYVTSGGNSMEWMAEVETAESLMSAETKTRWHKTMVRGVPIYLLVPR
                     GQKELAQKIAHEAEVSFTCIYEYSFLNDACHVL"
     gene            complement(3381..>6905)
                     /locus_tag="VI359_07990"
     CDS             complement(3381..>6905)
                     /locus_tag="VI359_07990"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY6974231.1"
                     /translation="RFTRRGQSPYEGLEFVKRSSEIRNPDGSTVFKLDQIDIPEPWSQ
                     LAIDILAQKYFRKAGVPLRDEHGNPILGQDGKPVSGGERDARQVFERLAGCWTHWGKT
                     YGYFKTPEDSEAFHDELCYMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHYYVDPKT
                     REVVKATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTNFSKLR
                     GDGESLSGGGRSSGLMSFLRIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDVEEFIDWK
                     VIEEQKVAAMVTGSKICAERLNAVLKACHVVDGAGQMKVEINAKQNPILKEALAAARR
                     DAVPEAYVQRMFAYAQQGFTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNEFFAAL
                     DADGDWQLKRRIDGKLSKTVKARDLWDRIAWAAWICADPGTQYDTTINEWHTCPEDGR
                     INASNPCSEYMFLDDTACNLASLNLAEFFNSDGRFELDNFRHAVRLWTIVLEISVLMA
                     SFPSRSIAEKSYHFRTLGLGYANLGTVLMRQGIPYDSPSALAICGALTAIMTGESYAA
                     SAEMAAELWPFPGYAKNRDAMLRVIRNHRRAAYHAPDEEYENLTIKPMGIQAEHCPPE
                     LLIAARRAWDRALELGTAYGFRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALVKFKKL
                     AGGGYFKIINQSLPPALTSMGYDESQIRDIVSYCVGRQTLQGAPFINHETLRQKGFDD
                     AALARLESGLAQAFEIQFAFNKYTLGEDFCRVKLGLTDAQLNESNFNMLKALGFTQEE
                     VAAANDYCCGTMTVEGAPHLKAEHLPVFDCANRCGRIGQRYIPVDAHIRMMAAAQPFI
                     SGAISKTINMPADATLDDVKSAYLFAWKSMVKAVALYRDGSKLSQPLSASTDSGKTAE
                     AAPEVLAAAEKITERVLVRYLAKRRPLPTRRNGYTQKAVIGGHKLYLRTGEYEDGTVG
                     EIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVKLNDRI
                     KMSTSIIDYIFRELAVTYLDRFDLAQVQEEDLRMDSMKKDDQDPECMEEEATPETLAS
                     ASISTELFPSRQNVTSRHQGNGHGHGNGSIAHKVEIKRETVTLTAVQIARQKGYEGDP
                     CSECKQFTMVRNGTCLKCDTCGATSGCS"
BASE COUNT         1593 a         1946 c         1711 g         1655 t
ORIGIN      
        1 atttgaacgc tgcgaatgga ccgtggatct gcttcatgga tcagaatgcg gcaattgccg
       61 atcttgagct catctccgac tttcggtatg cggcccaatt gttcctgaat aagaccgctg
      121 atcgttaccg cttcgtctcc tagatccact ttcaagaatt cgttgatttt atggacttcg
      181 gtccttccgt gaaccaggat ctgatttttc ccaagacgct tgatgagttc ttccgtaatg
      241 tctgtctcgt ccataatctc gccgaccacc tcttccagta gatcttcgag cgttacgatt
      301 cccatgacgc cgccgaattc gttcacgact accgccatgt ggcgtttatc ttgctggaac
      361 tgtttcatga gatcatcggc agtttttcct gccgggacaa acaaggccgg gttcgcgatg
      421 tcttttaatt tcacgtcaaa ccggcctttc gacaattccg tgagcgcacg cgtcttatag
      481 agaatcccgg tgatgttatc caacgttccg tcataaacgg gaacgcgcga gtattttgaa
      541 ttgtagagca gctcttgggc ttccttgagg tggagattcc cgtccaaaga aaacacatac
      601 aagcgtggcg tcatggcgtc ttccgccgtg atgtctttga gctggaacac gttcttgatc
      661 atcttaactt tctcgaactc gatcgccccg gctctccctc cctcatccag catgatcttg
      721 agctcttctt cagtcaccag cggtaaggtc agtcctttcc ctcctgtcag cttgtaaatc
      781 aacggcacca gcacggccag tatcggtttc agcagcagct gaactccata gacgggatag
      841 gccatattga gtgtgacggg aacggcaaat ttcgccgcta acgtctttgg aatgacgtct
      901 acggtaacca ggagcacgaa tgtcaataaa ccgacggcga cggcgacggt atcactaaaa
      961 aatgttcgct cctccccaaa catattgagt gtaaggaagg tggcatacat tggggttgcc
     1021 gtcgcaatga gtcgatcccc gactaagata gtcgatagga gcttttgggg gtcactccga
     1081 agctgtagag ccattttggc acgcttgctc ccattctggg cgatagcgcg cagcttcgtc
     1141 tcattaactg cgaaaaagcc gatctcagcc gtcgagatca cagcggaaag aacaataaga
     1201 ccaaggagaa ttaagatatc aatcatgtga gtctagaatt aggaacgccc gaccggtaaa
     1261 cccggtgaga ggcatatacg gcttggaaaa accgatcccc cagccatctg aacacgccgc
     1321 agggccaaaa ccacctggtc acccaggaag tggcttgcag actctgcgct gtgcatagga
     1381 cattacattt atcacaggag gggggagcca gcaagcagat catcaaaacc gtctaacaaa
     1441 tcaaatggtt gtgctgcatc tcttgataat ttcaaggaaa actccatccc atattctgct
     1501 aggtatcgtc gcgcacgaca aaaggctgga tatccaagga agattcgagg cgagaagccg
     1561 cagtttgggc ctgagcctcg gtcatgaact cccctacctg aacacggtaa cgacgcccct
     1621 ccggaagttc gatcgtcaca atccgccccc ccttgtagtt ggtcttcagc cgttccagca
     1681 gattcacggc attctgatga tcggcgaaag agcccacctg gatcctcaga acccccatat
     1741 cagccgtacg accctggtat ccgactacac gaagttctat ctgatcggtt cctgccccgg
     1801 tcatacccaa tgcatgggcc ccagctagtg aaagatcgac gatccgccct ctggcaaatg
     1861 gaccccgatc gttgatacga accgtaacgt gccttcccgt agtcaaggat cggacgacag
     1921 cgaccgagcc caagggaagc gtgcgatggg cggcagtcag cttgtacatg tcataccgtt
     1981 caccattggc agttttgttt ccgtgaaacc ccggaccgta ccaggatgcc acgcctcttt
     2041 cgacgtatcc aacggggtaa ccggggaaat aggttggacg gggagccgct cctccgcagg
     2101 aaaccagcag gtaactagtg ccaaccagga gggctatgac caatgggcgg gaccagacca
     2161 cggcgagccc tttccacatg gtcagaattc gtcaagggac tgatcccgca aaacgttgag
     2221 agccttttct gtattggaaa ccgttagaac cacgatcgcc cgtttgcctt cgcgcgaggg
     2281 ggtacaatag ccacacttaa tattaatccg tgctttggtt aagagatcgg ccacttgctt
     2341 caaggcgccc ggcttatttt ccaaactcag cagcagtgcc gtttcttcgg aaaacctgcc
     2401 cttcgcagct tttaatgcag ccctggctcc atcgagatcc gctacaagga tccgtaattt
     2461 tccggtccca gtcacttcgg gcgcagaaaa tgccttgatg ttcacaccgg cctcacccag
     2521 aacggacgcc acttgagcaa tgacgccagg tttgctttgt tcactaatca atagttgcgt
     2581 tgtcgttggc atcgacagca ctccaggtta taagggttca tcgaccacga cgcatcacga
     2641 ttcttaacac cttcacagtt taattttatc gactgcaatc agaacctgca acgacgattg
     2701 atcggttgac aagcaagtgt tctgtcgctg ttttactcag gagatcaccc actcccatgg
     2761 gtctcacgga tgaaccgcta gctttcaggg gcgcgcccac tggaacctaa cgtacccttt
     2821 cagttcaaaa ggtaatctca gaagtgggat tcaagctcgt cataaaacat gacaggcatc
     2881 gttgagaaat gagtactcat agatgcacgt gaacgatacc tcggcttcgt gggcaatctt
     2941 ctgagccagc tccttctgcc cacgaggcac caagaggtaa atcggaaccc ctcggaccat
     3001 tgtcttgtgc cagcgggtct tggtttcggc actcataagt gactcagccg tttcgacttc
     3061 agccatccat tccatactat ttccgccgga agtcacatac cagccgacga tgtcgcactg
     3121 ctgattggaa tcggcccagg gattttgttc aagactcgtc ttaatggtga cccggcaatg
     3181 aaaagcttgc gcccatcgtt gagccaccag gctgacgacc tgatcgtgga gcgtttcaat
     3241 gccctgttcc cgaaacgtcg tgctcatgaa tagcctctta gctgcaccgg ttggttatcc
     3301 cgccacagga ttactcgatt gcaccaggtc cagacatttt ctcgagttcc catatgaaaa
     3361 atccttctat tgggcgattc ctaagagcat ccacttgtgg caccacaggt atcacatttg
     3421 agacaggtgc cattgcgcac catcgtgaac tgtttacact ctgagcaagg atccccctca
     3481 tatccctttt gacgggcaat ttgtaccgct gtgagcgtca ccgtctctcg cttgatttcg
     3541 accttgtgtg caatgctccc gttcccgtgt ccatggccat tgccctggtg ccgggaggtc
     3601 acattctgtc tggacggaaa cagctcggtg ctgatcgagg ccgatgccag ggtttccgga
     3661 gtcgcttctt cttccatgca ttccggatct tggtcgtcct tcttcatcga atccattcgt
     3721 agatcctctt cctgaacttg cgccaggtcg aatcgatcaa gataggtcac ggccagttca
     3781 cggaaaatat agtcaatgat cgaggtcgac atcttgatgc gatcgttgag cttcaccgga
     3841 ccgttgggtt caaatctggt gaacacgaag gcttcaacga actcttccag cggcacccca
     3901 tgttgaagtc caagggaaat cgcgatggca aagcagttca tcaaactgcg gaaggcagca
     3961 ccctctttat gcatgtccaa aaagatttcg ccaacggtcc catcttcata ttcaccggta
     4021 cggaggtaga gtttatgtcc gccgatgact gccttctgcg tatagccgtt gcggcgtgtt
     4081 ggaagtggcc gccgtttagc caaatatcga accagaaccc gttccgtgat tttttctgca
     4141 gccgcgagca cttcgggggc ggcttcggcg gttttcccgc tatcggttga ggcggacaat
     4201 ggctgactta attttgaccc gtcgcgatac aacgcaactg ccttcaccat cgatttccag
     4261 gcaaagagat atgccgactt gacgtcatcc agcgtggcgt ccgccggcat gttgatggtc
     4321 ttgctgatcg cgccgctgat gaacggttgt gcggcagcca tcattcggat gtgggcatcg
     4381 acgggaatat atcgctggcc gatacgcccg catcgattgg cacaatcaaa cacgggcagg
     4441 tgctcggctt tgagatgcgg cgcaccctca accgtcatcg ttccgcaaca atagtcgttc
     4501 gctgcagcga cctcttcttg cgtgaatccc agtgccttca gcatgttgaa gttggactca
     4561 ttgagttgcg catcggtcaa tccgagtttt actcggcaga aatcctcgcc cagggtatat
     4621 ttattgaacg caaactgaat ctcgaaagct tgcgcaagtc cgctttccag gcgggccaga
     4681 gcggcatcgt caaatccctt ttgtcggaga gtctcatggt taatgaacgg cgcaccttgc
     4741 agggtctgac gcccgacaca atagctcacg atgtcgcgaa tctgagattc gtcataaccc
     4801 atggatgtca acgccggcgg caaactttga ttaatgattt tgaaataacc accacccgcc
     4861 aactttttga atttcaccag cgcaaaatcc ggttcgatgc cggtggtatc acagtccatg
     4921 accaagccga tcgtacccgt cggtgcaatg accgtgactt gcgcatttct gaacccgtac
     4981 gccgtgccga gctccagggc gcggtcccat gctcgtctcg cagcaatcaa cagttccggc
     5041 ggacaatgtt ccgcctgaat acccattggc ttgatggtca ggttttcata ttcctcgtca
     5101 ggagcatgat aggctgctcg acgatgattc cggatcaccc ggagcatcgc atcacgattt
     5161 ttcgcataac cgggaaacgg ccagagctca gccgccattt cagccgatgc cgcatacgat
     5221 tcaccggtca tgatcgcagt caaggcgccg cagatcgcca acgcactagg agaatcgtaa
     5281 ggaattccct gtcgcatcag aactgtgccc aaattggcat accccaaacc aagagttcgg
     5341 aagtggtaac ttttctccgc aatcgagcgg ctgggaaacg atgccatcaa cacgctgatt
     5401 tccaatacaa tcgtccagag acgaacggcg tgccggaaat tatccagctc aaaacgccca
     5461 tcagagttaa aaaactccgc cagattcagc gaagcgagat tgcatgccgt atcatcgagg
     5521 aacatgtatt ccgagcacgg attcgaagca ttgatccggc catcctccgg acaggtatgc
     5581 cattcgttga tcgtggtgtc gtattgggtg ccgggatccg cacaaatcca tgccgcccag
     5641 gcgatacgat cccataaatc acgtgccttc accgtcttgg acaatttgcc atctatccgt
     5701 cgtttcaatt gccaatcgcc gtcggcgtcc aatgccgcaa aaaactcatt gggaatgcgg
     5761 acactgttgt tcgagttttg acccgagacc gtctgatacg ccttgccatc ccaattggtg
     5821 tcgtattcgt gaaacacgaa atgggtaaac ccctgctgag cgtaggcaaa catccgctgc
     5881 acataggcct ctggaaccgc gtcgcgcctc gctgccgcca gggcttcctt gagaatcggg
     5941 ttttgcttcg cgttgatctc aactttcatc tgtccggcac cgtcgacaac atggcaggcc
     6001 ttgagaacgg cattcaggcg ttccgcgcag atcttggatc ctgtcaccat ggcggcgact
     6061 ttttgctctt caattacctt ccaatcgatg aactcctcga catccgggtg atcaaggtcc
     6121 aaacagacca tcttggcagc acgtctggtc gttccgccag acttgatggc gccagccgcg
     6181 cggtccccga tgcggaggaa cgacatcaaa ccggatgaac gcccccctcc agatagtgat
     6241 tcaccgtccc cgcgtagttt cgaaaaattg gttccggttc cggatccgta cttgaacaag
     6301 cgagcttccc gaacccagag atccatgatg ccgttttcat tgacgagatc gtcctcaatt
     6361 gactgaataa agcaggcgtg cggctgggga tgttcaaacg cgtttgtggc tttcacgacc
     6421 tcgcgagttt tcggatcgac ataatagtgt ccctgcgctg gtccacttaa tccataagcg
     6481 tagtgaagac ctgtgttgaa ccattggggc gagttcggcg cagccatttg atgcgccagc
     6541 atgtaacaaa gttcgtcatg aaaggcttcg ctgtcttcag gcgtcttgaa atacccgtag
     6601 gttttccccc agtgagtcca acatcctgcc agtcgctcga acacctgtcg cgcatcgcgc
     6661 tctcctcctg agaccggctt gccgtcttgc ccaagaatcg ggttgccgtg ttcatcacgg
     6721 agtggaacac cggctttccg aaagtacttt tgcgcaagaa tgtcaatggc cagctgagac
     6781 cagggttccg ggatatcgat ctgatcgagc ttgaagacag tcgagccatc gggattccga
     6841 atctccgagg aacgtttcac gaactctagg ccttcatagg ggctttgtcc gcgacgcgta
     6901 aatcg
//
LOCUS       DATDNW010000334         4328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_517773, whole genome shotgun sequence.
ACCESSION   DATDNW010000334 DATDNW010000000
VERSION     DATDNW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517773"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VI359_07995"
     CDS             <1..616
                     /locus_tag="VI359_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY6974232.1"
                     /translation="EIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGLIMI
                     GPRFGGAVTDAGRYFKYAVDNKMSVDEFLAYMKKSVGPVPGIGHRVKSLRNPDKRVKE
                     LVGYVKSLNMKTPCLDFALEVEKVTAAKKDNLILNVDGTMAAVLVDIGFPVDSLNGFF
                     ILSRTIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            668..2914
                     /locus_tag="VI359_08000"
     CDS             668..2914
                     /locus_tag="VI359_08000"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY6974233.1"
                     /translation="MSLELARKLYAKMPDVFAKARKKFGRGLTLAEKVLVSHADNFET
                     QAWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVAYMNITD
                     RADLANLAQSHKELLVADPDVYQSPEKYYDQIVEVNLSTLEPHVVGPHTPDLARPISK
                     LAAEAKEKGYPVELKAALIGSCTNSSYEDISRSAHIARQGLKAGMKAKTSFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSFLASPEVVTAYALSGDLGFDPVNQKLKGADGKEFKLEPPVGEELPAKGF
                     AKGEEGFVAPAENGEALTVDIPPTSERLQLLQPFPRWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANNAFASEPGKGTDVLTGESGLTVAQIARRYKA
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRVVITKSFARIHETNLKKQGILALTFA
                     DPKDYEKIEQQDRISVTGLQSLAPGKPVQVIIHKTDGKALTIQANHSITEQQIAWFKA
                     GSALNALN"
     gene            2941..>4328
                     /locus_tag="VI359_08005"
     CDS             2941..>4328
                     /locus_tag="VI359_08005"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY6974234.1"
                     /translation="MTTKASKIIYTKTDEAPMLATYSFLPIINAFSKAAGVSVELRDI
                     SLAGRILAVFPEYLTPQQKQADALSELGELAKTPEANIIKLPNISASLPQLNAAIKEL
                     QSQGYKLPEYPENPKDDKEKDIKARYDKVKGSAVNPVLREGNSDRRAPLSVKAHSRKH
                     PHKMGAWSPDSKTHVSHMKGGDFRSNEKSMTTTAATDARIEFVGQDGKTTVLKPKVAL
                     QAGEVIDATFMSRRALRQFLEEQIEDAKKQGVLFSLHMKATMMKVSDPKIFGHAVTVY
                     YKDVFEKHGETLKKLGVDPDNGIGDLYAKIKALPDDQRKAIEADIQEVYKKRPPMAMV
                     NSDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKPADAKCVIPDGSYAPVYHEIV
                     EFCKKHGAFDPRTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRVVDASGKTLLEH
                     KVEEGDIWRMCQV"
BASE COUNT         1026 a         1172 c         1325 g          805 t
ORIGIN      
        1 cgagatcatc aagcgaatca tgatgctctc cgccgaccat ggtccttgcg tcagcggggc
       61 ctatgcgacc atcttggcgg cttgcgccgg gatcgggttg tcacagtcgg tggcagcagg
      121 cttgattatg atcggtccgc gcttcggcgg cgccgtcacg gatgccggtc gatacttcaa
      181 gtatgcggtc gacaataaga tgtccgtcga cgagttcctg gcctacatga agaagagcgt
      241 cggcccggtg ccggggatcg gtcatcgcgt caagagcctg cgcaatccgg acaagcgggt
      301 aaaagagctg gtcggatatg tgaagagctt gaacatgaag acgccctgtc tcgattttgc
      361 gctggaagtt gaaaaggtga cggcggccaa gaaggataac ctgattctga acgtcgacgg
      421 gacgatggca gcggtgctgg tcgacatcgg cttccctgtc gatagcttga acggattctt
      481 cattctgtcg cgcacgatcg ggttaatcgg ccactgggtg gatcagaagc gccaggatag
      541 ccgcctgatc cggctctttg attacctggt aaactatgca gcgcccaagc ggcgcgaagt
      601 gccgccgctg aaataaagcc ggcaaccgat cgctttcagc catcagccaa actgaacgga
      661 gatagccatg tcactcgaac tcgcaagaaa actctacgct aagatgccgg atgtgttcgc
      721 caaggccaga aagaaattcg gccgtggtct gacgctggca gagaaagtgc tggtttcgca
      781 cgccgacaac ttcgagacgc aggcctggga gcggggcaaa gccatgttgg cgctccgccc
      841 ggatcgcgtc gccatgcagg acgccacggc ccagatggcc atgttgcagt tcatgcaggc
      901 gggcaagaag aaagcggcgg tacccagtac gattcactgc gatcacttga tccgtgccga
      961 aatggggtcc gagaaggacc tgcttcgcgc catggatgag aacaaggagg tctacaactt
     1021 tcttgcgtcc gctgcgaaga agtacggcat tggattctgg aagccgggag cgggcatcat
     1081 ccaccaagtc gttctggaga attacgcgtt tcctggcggc ctgatcatcg ggaccgactc
     1141 gcacacgccg aatggtggtg gactgggcat gttggccatc ggtgtcggcg gtgccgacgc
     1201 aggcgaagtc atggccggcc tgccgtggga agtactccat ccgaaattga tcggtgtgcg
     1261 tctgaccgga aaactgagtg gctgggcctc accgaaggat gtcattttgt atctctgcgg
     1321 tctgttgacg gtgaaagggg gcacgaacaa gatcgtcgag tactttggtc cgggtgccga
     1381 gaccatcagc gcgaccggca agggcacgat ctgcaatatg ggcgcagaac ttggagccac
     1441 gacgtctgtg ttccccttcg atcagaagat ggtcgcctac atgaacatca ccgatcgggc
     1501 ggacctggcg aacttagcgc aatcgcacaa agagctcctc gtggccgatc cggacgtgta
     1561 tcagtcgcct gagaagtact acgatcaaat cgtggaagtg aatctttcga cgctggagcc
     1621 gcatgtcgtc gggccgcata cgccggatct ggctcggccg atttccaagc tggcggcgga
     1681 ggcgaaggaa aaggggtatc ccgtcgagct gaaggcggcg ttgatcggca gttgtacgaa
     1741 ctcatcctat gaagacatca gccggtccgc ccacattgcc agacaagggc tcaaggctgg
     1801 catgaaagcc aagacgtcct tcctcgtgtc gccgggctcc gagcgtatct atcacacgat
     1861 gaagcgcgat ggcttcctgg atacgttcga gaagttgggc ggaaccgtcc tgtcaaattc
     1921 ctgcggcccc tgcatcgggc agtggaagcg agccgatggc gtgaagggca aggcggattc
     1981 gatcgtcagt tcgttcaacc ggaacttccc gggccgcaac gacggcatca acgaaacgtt
     2041 gtcgttcctt gccagtccgg aggtcgtgac cgcctatgca ttgtccggcg atctcggatt
     2101 tgatccggtc aatcagaagc tcaagggtgc cgacgggaag gagttcaagc tagaaccgcc
     2161 ggtaggtgaa gaattacctg cgaaaggatt tgcaaaagga gaagaggggt tcgtagcacc
     2221 ggcggaaaac ggcgaggcct tgaccgtcga catcccaccg acgagcgaac gattgcagtt
     2281 gttgcaaccg tttccacgtt gggatggcaa agatttcgag aagctcccgc tcttgatcaa
     2341 gaccaaaggc aagacgacga ccgaccatat ttctccggcc ggtccgtggc tcaagtttcg
     2401 cgggcatctc gacaagatca gcgacaacat gttcctggga gcgaacaacg cgttcgcgtc
     2461 ggagccgggg aagggcacgg atgtcctgac cggtgaatca ggcctgacgg ttgcgcaaat
     2521 cgccagacgg tataaggcaa agggaatcgg ctcgatcgtg gtcggcgacg agaattacgg
     2581 cgagggcagc agccgcgagc acgcggccat gtcgccccgc ttcctcaatg tgcgtgtcgt
     2641 catcacgaag agcttcgcgc gcattcatga aaccaacttg aagaagcaag ggatcttggc
     2701 gttgaccttc gctgacccga aggattacga gaagatcgag cagcaggacc gcatcagtgt
     2761 gacggggcta caaagcctgg cccctggcaa gccggtgcag gtgatcattc acaagacgga
     2821 tggcaaggcg ctcaccatcc aggcgaacca cagcatcacc gagcagcaga ttgcgtggtt
     2881 caaggctggt tcggcgttga acgcgctgaa ctgacacacg atgcggaaag aggaaaaacc
     2941 atgacgacaa aagcatccaa gatcatttat accaaaaccg acgaagctcc gatgttggcg
     3001 acgtactcgt ttctgccgat cattaacgcg ttcagcaagg cagccggcgt atcagttgaa
     3061 ttgcgggaca tctcgctggc cggtcgcatc ctggccgtgt ttcccgaata cctgacgccg
     3121 cagcagaaac aggcggacgc gctgtctgaa ctgggcgaac tcgccaagac gccggaagcc
     3181 aatatcatca agctgccgaa cattagcgcc tcgcttcccc aattgaacgc agccatcaag
     3241 gaattacaga gccagggcta taagcttccc gagtatccgg aaaatccgaa agacgataaa
     3301 gagaaggaca tcaaggcccg ctacgacaaa gtcaaaggca gtgccgtcaa cccggtcttg
     3361 cgtgaaggca actcggatcg ccgcgcgccg ctctccgtga aagcccattc caggaagcat
     3421 ccacataaga tgggggcttg gagcccggat tccaagaccc acgtctctca tatgaagggc
     3481 ggggatttcc gctcgaacga aaagtcgatg acgaccaccg cggcgaccga cgcgcggatt
     3541 gaattcgttg gccaggatgg taagaccact gtgctcaagc caaaagtggc gctgcaggca
     3601 ggggaagtca tcgacgcgac attcatgagc cgtcgcgcgt tgcgacaatt cctcgaggag
     3661 cagatcgaag acgccaagaa gcagggcgtc ttgttttcgc tccatatgaa agccaccatg
     3721 atgaaggtct cggaccccaa aatcttcggt catgccgtga cggtgtatta caaggatgtg
     3781 ttcgagaagc acggcgagac gctcaagaag ttgggtgtgg atccagacaa tggcatcggc
     3841 gacctttacg cgaagatcaa agccttgccg gatgatcaac gcaaagcgat cgaagccgat
     3901 atccaggagg tttacaagaa gcggcctccc atggcgatgg tgaattccga taaagggatc
     3961 accaacctgc acgtgcccag cgacatcatc atcgacgcct ccatgccgcc ggttattcgc
     4021 gacagtggca agatgtgggg cccggacgga aagccggcgg acgccaagtg cgtcattccc
     4081 gatggcagct acgcgccggt ctatcacgag atcgttgaat tttgtaaaaa gcatggcgcc
     4141 ttcgatcccc gtacgatggg cagcgtgccc aacgtcggtc tcatggccca ggcggcggag
     4201 gagtatggtt cgcatgacaa gaccttcaag gctccgggca acggcacgat ccgagtggtc
     4261 gatgcatcag gaaagacgtt gttggagcac aaggtggaag aaggcgatat ctggcgcatg
     4321 tgccaggt
//
LOCUS       DATDNW010000335         7227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_873949, whole genome shotgun sequence.
ACCESSION   DATDNW010000335 DATDNW010000000
VERSION     DATDNW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873949"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VI359_08010"
     CDS             complement(<1..842)
                     /locus_tag="VI359_08010"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY6974235.1"
                     /translation="MLEPSAKIWMDGRFVNWADANVHVLTHSLHYGLAAFEGVRCYKG
                     RSGSAIFKLHDHVDRLFESAHIGMMQIPYDKKQVTDAIVETVRVNKLDACYIRPLVYI
                     GYGAMGVYPAENPIKLAIAAWKWGAYLGDEALANGMRARVSSFTRHHVNVSMTRGKIS
                     GYYVNSILAKLEVKADGYDEAILLDPEGYVSEGTGENIFIVRKGRLKTTPLTSILEGI
                     TRNAVIELAREQQISVAEERFTRDEMYIADEVFVTGTAAELTPVREIDNRRIGTGKPG
                     PITLA"
     gene            complement(954..1334)
                     /locus_tag="VI359_08015"
     CDS             complement(954..1334)
                     /locus_tag="VI359_08015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974236.1"
                     /translation="MNEMTKPYYQKKVTQFILRPFRRIPTWCVVHYLGGEFIGKGIMT
                     NLSQSGMRVQGDHTVTPGMQIAIRLIFAENGSSLQIERATVRWVNGYDFGLEFERIAP
                     MASKQIAHVISTNGRSFPTPFYTV"
     gene            1542..1856
                     /gene="rplU"
                     /locus_tag="VI359_08020"
     CDS             1542..1856
                     /gene="rplU"
                     /locus_tag="VI359_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY6974237.1"
                     /translation="MYAIIETGGKQYRVEAGAQVQVESLPGDVGGTIELNHVRLVHGD
                     KGVVIGQPLVAGAKVIAEIIRQGRTRSITVFKKQRRKNYRRTRGHRQGFTRLRITGIE
                     TA"
     gene            1869..2132
                     /gene="rpmA"
                     /locus_tag="VI359_08025"
     CDS             1869..2132
                     /gene="rpmA"
                     /locus_tag="VI359_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY6974238.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGETVKAGAIIVRQRGTK
                     FFPGFNVGLGKDHTLFALIQGVVKFEGGRARQKVSVYPAPASL"
     gene            2256..3281
                     /gene="obgE"
                     /locus_tag="VI359_08030"
     CDS             2256..3281
                     /gene="obgE"
                     /locus_tag="VI359_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY6974239.1"
                     /translation="MFVDEVRIFVKAGRGGDGACSFRREKYVPRGGPDGGDGGHGGDV
                     VLTASARLSTLLDLRYQQQYEAQDGEAGGGSNCSGKRGENVALAVPVGTVVYDDSTNE
                     VVADLVASGDAFVGAHGGRGGRGNSHFATSTNRVPTQFETGTDGEERWLRLELKLLAD
                     VGLVGFPNAGKSTLIAAISAARPKIADYPFTTLTPNLGVVRWGNDRTFVVADIPGLIE
                     GAHEGKGLGFQFLRHIERTSFLLHLIDVSEWAPDDPVTSFEVMRQELRAYDEKLAERP
                     FAVVGTKIDITGDGTRLHQLQTFCKRHRYKYFPISAATRDGLTELVTYVGQQVESLRH
                     TPCETNS"
     gene            3263..4384
                     /gene="proB"
                     /locus_tag="VI359_08035"
     CDS             3263..4384
                     /gene="proB"
                     /locus_tag="VI359_08035"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY6974240.1"
                     /translation="MRDELLTHAKRIVIKIGSSLVASRESGLRPEQIDRLADDIASLR
                     SGGREILLVSSGAIVSGIKKLGLKEYPKSLPVKQAAAAVGQSRLMWAYEKSFERCGAK
                     VAQILLTHHDLTDRRRFLNARHTLTALIGFGVIPIINENDTVAVDEIRVGDNDTLAAD
                     VAHLVDADLLVILSDVEGFFTDDPRKNPAATLIALVPEITDDIERRAGMSSTFEGTGG
                     MATKIRAAKKVGEYGVATLILNGEKSGLLSTVLAGGAGGTLFCAKERRLTSRKHWIAF
                     TLRPRGHLILDDGAVEALARRGKSLLASGILGVTGSFDAGDPVSCTDRDGKEFAKGLV
                     NFSSDTLAQIKGLKTTEIHLRIGPQEYEEVIHRDNLVIL"
     gene            4457..5116
                     /gene="nadD"
                     /locus_tag="VI359_08040"
     CDS             4457..5116
                     /gene="nadD"
                     /locus_tag="VI359_08040"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY6974241.1"
                     /translation="MTRLGLLGGSFNPVHNGHLAIARQTRDLLGLNQILFIPAGDPPH
                     KPIGSLASAKDRYEMVRLAIACDPTFTVSNVEINRPGKSYTLDTIRTLRQQYGPATSL
                     FFLIGLDAFLDFPGWREPATLLTLCSFVVISRPGISFQSLTSMTLLPAIPEESLVALD
                     AGRMLKLEVPLSHQQLICIHVPPTPVSASDLRHRVRQGQSVANLLPPSVESYIIQHHL
                     Y"
     gene            5127..5549
                     /gene="rsfS"
                     /locus_tag="VI359_08045"
     CDS             5127..5549
                     /gene="rsfS"
                     /locus_tag="VI359_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY6974242.1"
                     /translation="MARTSKATALAVARAMLDKKATDVVVLHVAKLTSVADYLVLGSA
                     ESDRQTRAIADHVDGILSSSGSGPLSIEGTASSPWVLIDFGDVVAHIFRHDIRQHYAL
                     ERLWADAKRVRVPDESASRPMTTTRTRQVRKTRATKQV"
     gene            5554..6954
                     /locus_tag="VI359_08050"
     CDS             5554..6954
                     /locus_tag="VI359_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6974243.1"
                     /translation="MLRLLLTIFLISTGIFVYSYFRELNPGTLMIRTGPSVQFELSPV
                     TLVVFSMAVGAVLVAFAVGMRQTVHVIGNWRNTRMLRRREKLDALHREGTHAFMSKRT
                     ADAIGLFEKALAMDPNRTDSLLWLGNIYRSENNFAEAIRLHQHANRIDDRNVEVLLEL
                     TKDLEGAKRYEDALQAYYRILKIEPDNLTALIRKRDLLIRLEKWSEALEIQHRLVKAP
                     LPESERRAETHLLTGCMYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGLGEIL
                     LREGKTRDAVEILKKVYAKTRSIIILHRLEELFLEQGEPSEIIRIYQEALQQDPHNTA
                     LQFYLGKLYYRLEMIDEAFDILSTIEGPQDQMMDYHKIMANLFLRKQHMEQAVVELKK
                     ALHFKKRVVVPYVCTACQQESVEWSGRCRRCGNWNTFVSLPGPDSGQSVTGQDDRPTS
                     VRTVPYQGIASPFETV"
     gene            7028..>7227
                     /locus_tag="VI359_08055"
     CDS             7028..>7227
                     /locus_tag="VI359_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY6974244.1"
                     /translation="MVDYQELANKAVRAELLNRSECQTVLNTPDDDLLALLQAAFTVR
                     SRYFGKTVRLQMLQNAKSGACQ"
BASE COUNT         1640 a         2059 c         1971 g         1557 t
ORIGIN      
        1 gcaagcgtga tgggtcccgg ctttccggtt ccaatgcggc ggttatcgat ttcacggacc
       61 ggtgtcaatt cggcggccgt acctgtcaca aaaacttcat cggcaatgta catttcatcg
      121 cgggtgaacc gttcctcggc cacggaaatc tgctgttcac gagccaactc gattacggca
      181 ttcctggtga tgccctccag gatcgacgtc aatggtgtcg tcttaagccg tcccttccgc
      241 acgatgaaaa tattctctcc cgtgccttcg gaaacataac cctcggggtc caatagaatc
      301 gcttcgtcat acccatccgc tttgacttcg agtttggcga ggattgaatt tacgtaatac
      361 ccagagattt tccctctcgt catcgaaaca ttgacatgat gacgagtaaa ggaggacaca
      421 cgagcacgca tcccgttggc gagcgcttcg tcgccgagat aggcgcccca tttccacgct
      481 gcgatggcaa gcttgatggg attctccgcc ggatagactc ccatcgctcc atagccgatg
      541 tacaccagcg gacgaatgta gcacgcatcc aatttgttca cgcggaccgt ctcgacgatc
      601 gcatccgtca cctgcttctt gtcataggga atctgcatca tcccgatgtg agcagactcg
      661 aaaagccgat cgacatggtc gtggagcttg aaaatagccg atccggacct tcccttgtag
      721 catcggacgc cttcgaacgc ggcaaggccg taatggagcg agtgcgtcag cacatggacg
      781 ttcgcgtccg cccagttgac gaagcggccg tccatccata tttttgcact tggttcgagc
      841 atggttcggt gaagattctc tttaagaata tgacgatgag cggactatag cagtgggccg
      901 gaagaagcgt caagcaaacc aatagacgca acggattccg agctcaggcg ttgtcaaacc
      961 gtgtaaaaag gagtggggaa ggaacgaccg ttcgtactga tcacgtgagc aatctgcttc
     1021 gaggccattg gagcaatgcg ttcaaattcg agaccgaagt catacccgtt gacccatcgg
     1081 accgtagccc gttcgatctg gagggagctc ccgttctcgg caaagatcag ccgaatcgca
     1141 atctgcatgc cgggtgtcac tgtatgatcc ccttggacac gcatgccgga ttgggacaag
     1201 tttgtcatga tacctttccc gatgaattca ccgcctaagt aatgaacgac gcaccacgta
     1261 ggaattcggc ggaagggtct caagataaac tgcgtcacct tcttctggta atacggttta
     1321 gtcatttcat tcatcagacc acacctctct catcttgact tacacctcgc actataccca
     1381 ggcgcaccac acttcgacag cccacaaaca gggtagccct ttggtagggg ttcataggtg
     1441 attcgtgcaa gtaaacaggt tattagggat gacacgctgc attgacacga ttcaaatccc
     1501 tatgatataa gggtgctttt cgtcatggag gcgggagaag catgtacgcg attattgaaa
     1561 cgggcgggaa acagtatcga gtggaagcgg gagcacaggt gcaggtagaa agcctccccg
     1621 gtgatgtcgg cgggacgatc gaactgaatc acgtgcggct cgtccatggc gacaagggtg
     1681 tcgtcatagg tcaaccattg gtcgcaggtg caaaggttat agctgagatt attcgccagg
     1741 gccgaactag gtcgattacc gtcttcaaga aacaacgacg gaagaactat cgccggaccc
     1801 gtggacaccg gcagggcttc acaagactac gtattaccgg tatcgaaaca gcctaacgag
     1861 gacacgtcat ggcaacaaat aaaggcggcg gatcatcacg aaacgggcga gacagcaatc
     1921 cacaatatct cggagtcaag gcctacggcg gtgaaaccgt caaggctgga gccatcatcg
     1981 tccgtcaacg tggaacgaaa ttctttcccg gcttcaacgt gggcttgggc aaggatcaca
     2041 ctctctttgc ccttattcaa ggggtggtga agtttgaagg tggtcgcgca cgtcaaaaag
     2101 tgagcgttta tcctgcccca gcaagtttgt aagtcacgtt ctcattttct tgcaacacct
     2161 gttttcctag acaatgaggg gtcgcgcgca gctctcgcgc ggagtggggt atactgtctc
     2221 ccttctggca ggacctttcg taatgggtgg tcttcatgtt tgtcgatgaa gttcggatct
     2281 tcgtcaaagc cggccgtgga ggtgacggcg cctgcagctt tcgccgggag aagtatgtcc
     2341 cgcgcggagg gcctgacggg ggtgatgggg gtcacggagg agacgtcgtc ctgacggctt
     2401 cagccagatt gtcgacactc ctggacctgc gctaccaaca gcaatatgaa gcccaggatg
     2461 gtgaagcggg cggcggctcg aactgttccg gtaaaagagg agaaaacgtc gccctggccg
     2521 tccctgtcgg cacagtggtg tatgacgata gcacgaacga ggtcgtcgct gatttggtcg
     2581 catccggcga tgccttcgtg ggagcacacg gcggacgagg cggtcgcggc aacagtcatt
     2641 ttgcgacttc gacgaaccgc gtccccaccc agttcgagac gggaacggat ggagaggagc
     2701 gctggctccg actcgagttg aaactgcttg ccgatgtcgg gctcgttggt ttcccaaacg
     2761 cgggcaagtc gactttgatc gctgccattt ctgcggcacg cccgaaaatc gccgactatc
     2821 cgttcacaac gctgaccccg aacctcggcg tggttcgatg gggcaatgat cgcacgtttg
     2881 ttgtagcgga tattcccggt ctgatcgaag gagcccatga agggaaaggt ctgggattcc
     2941 aatttctccg ccacatcgaa cgcacctcct ttctgcttca cctgatcgat gtctcggaat
     3001 gggcccccga tgatcccgtc acgagtttcg aggtcatgcg ccaggaactc cgtgcctacg
     3061 acgaaaagct ggcagagcgt ccgtttgcag tggtggggac taagatcgac atcacgggtg
     3121 atggcacacg tctccatcaa ctacagacgt tctgcaaacg ccatcggtat aagtatttcc
     3181 ctatctcggc cgcgacaaga gatgggctga ccgaattagt gacgtatgtt ggacaacagg
     3241 tcgagagtct gagacacact ccatgcgaga cgaactccta acccacgcca aacggatcgt
     3301 catcaagatc gggagcagcc tcgtcgcatc ccgcgagtca ggactgcgcc cggagcaaat
     3361 cgaccgactt gccgatgata tcgcgagcct tcggagcgga gggcgagaga ttctactcgt
     3421 ctcttccggt gccatcgtct ccggtatcaa gaaactgggc ttgaaggagt atcccaaaag
     3481 tcttccggtg aaacaggccg ctgcggccgt gggacaaagt cgtctgatgt gggcctatga
     3541 aaaatccttt gagcgatgcg gagccaaagt ggcgcagatt ttgctcaccc atcatgacct
     3601 gaccgaccga cgccgttttc tgaacgcccg gcacacgctg accgcgctga tcgggttcgg
     3661 cgtcattccc atcatcaatg aaaacgacac cgtcgcggtg gatgaaattc gtgtgggaga
     3721 taacgatacg cttgcggctg atgtcgccca tttagtggac gccgatcttc tcgttatttt
     3781 gtcggacgtc gagggattct ttacggacga tcctcgcaag aacccggcgg cgacattgat
     3841 cgcgctggtc ccggagatta ccgacgacat cgagcgccgg gcaggaatgt caagcacgtt
     3901 tgaaggcacc ggcggcatgg ccaccaagat ccgggccgca aaaaaagtcg gtgagtacgg
     3961 tgttgcgact ctgatcctca acggcgagaa atcagggctc ctatccacag tattggccgg
     4021 tggggcgggc ggcacacttt tttgcgcgaa agaacgtcgc ctgaccagcc gcaaacattg
     4081 gatcgcgttt accctgcgcc cccgtggaca cctcatcctc gacgacggag cagtggaagc
     4141 cctcgcccgt cggggcaaga gcctcttggc gtccggcatt ctcggtgtca ctggctcctt
     4201 cgacgccggc gatccggtca gttgtactga tcgtgacgga aaggagtttg cgaaaggact
     4261 ggtgaatttc tcctccgata cgctggcgca aatcaaagga ctaaagacca cggagatcca
     4321 cctgcggatc ggcccccagg aatatgagga agtgattcac cgcgataacc tcgtgattct
     4381 ctagtgctga gaccgagcga tgatttccgc tgattctgaa cctcggccct tcgcatccag
     4441 ctccgagcac ttgagtgtga cacgtctcgg tcttctggga ggcagcttca accccgtcca
     4501 caacggccac ttggccattg cacgacagac gcgagacctc ctcggcctca atcagattct
     4561 cttcattccc gccggcgacc ctccgcataa gccgatagga tccttggctt cagccaagga
     4621 tcgatatgaa atggtccgac tggccattgc ctgcgatccc acctttaccg tctccaatgt
     4681 agagatcaac cgccccggca agtcctacac gctcgatacc attcgaacat tgcggcagca
     4741 atacggccca gcgactagcc tgttttttct gatcggtctc gacgcgttct tggatttccc
     4801 cggttggcgg gaaccagcaa cccttctgac cctttgttcg ttcgtcgtga tttcacgacc
     4861 ggggatctcc ttccaatcct tgaccagcat gacgctgctc ccggcgattc cggaagaatc
     4921 gcttgtcgcc ttggacgcag ggcggatgtt gaagctcgag gtgcctctct cacatcaaca
     4981 gctcatctgt atccatgttc ctccaacccc agtctccgcc tcggatctca ggcacagagt
     5041 tcggcagggc cagtcagtgg cgaatctgtt gccgccatcc gtagaatctt atataattca
     5101 gcatcatctt tactaagggg acgccgatcg cacgaacatc gaaggccact gcgcttgctg
     5161 tcgcaagggc gatgctcgac aaaaaggcca cggatgtcgt tgtcctccat gttgcgaaac
     5221 tgacctctgt cgccgactac ctggtcctgg gttcagcgga atctgatcga cagacacgtg
     5281 caattgctga tcatgtggat ggaattttgt cctcatccgg ttccggtccg ttgagcatcg
     5341 aagggaccgc ctcctcgccg tgggttctga tcgatttcgg cgacgtcgta gctcatattt
     5401 tccggcacga tattcgccag cattacgcgc tggagcggtt atgggccgac gccaagcgtg
     5461 ttcgtgtgcc ggacgaatcg gcgtcacgac cgatgacgac aacacggaca cgacaggtac
     5521 ggaaaacgcg cgcgacaaag caggtctaac gccatgctgc gactcctcct cactattttc
     5581 ctgatcagta ccggcatctt cgtctatagc tacttccgag aactcaaccc gggaactctc
     5641 atgattcgga cggggccgtc cgttcaattc gaactcagcc ccgtaacctt ggtcgtcttt
     5701 tccatggctg tcggagccgt gcttgtggca ttcgctgtcg gcatgcggca gacggtccat
     5761 gtaattggga actggcgcaa caccagaatg cttcgacgca gagaaaaact tgacgctctc
     5821 catcgcgaag gaactcacgc ctttatgtca aagcgcacag ccgatgctat cggattgttc
     5881 gagaaagcgt tggccatgga ccccaaccgg accgattcgc tgctctggct cggcaatatt
     5941 tatcgttccg agaacaactt cgctgaggcg atccgcctac accaacatgc caatcgaatc
     6001 gacgaccgaa acgtcgaagt cctgctggaa ctgacgaagg acttggaagg cgccaaacgc
     6061 tatgaagatg cgctccaggc ctattacaga atcctcaaga tcgaaccgga caacctgacg
     6121 gccctgattc gaaagcgcga tcttctcatc aggctcgaga aatggagcga agcgctcgaa
     6181 atccaacacc gcctggtgaa ggcgccgctg cccgagtcgg agcggcgagc cgagactcat
     6241 cttctcactg gctgcatgta cgaagtcgga cgtcaattgc tcgaacgagg gcacccggac
     6301 aaggcccggc gctattttcg tggagccatc aagaaagatc gaacctttct cccggcctat
     6361 attgggctcg gcgagattct ccttcgagaa ggcaagacga gggatgcagt cgaaattctc
     6421 aagaaagtct atgccaagac ccgcagcatc atcatcctgc atcggctcga agagctcttt
     6481 ctcgagcagg gggagcccag cgaaatcatc cgaatctacc aagaggcgct ccagcaggat
     6541 cctcacaaca ccgccttaca attctatctg ggcaagctct actacaggct ggaaatgatc
     6601 gacgaagcct tcgatattct ctcgacgatc gagggcccgc aggaccagat gatggactat
     6661 cataagatca tggccaacct cttcctgcgc aagcagcaca tggagcaggc cgtggttgaa
     6721 ctgaaaaaag cccttcactt taagaaacgc gtcgtcgtcc cctatgtgtg caccgcttgc
     6781 cagcaagaat cggtcgaatg gtcaggccga tgtcgccgct gcgggaactg gaatacattc
     6841 gtctcgctgc ccggacctga cagcggccag tcggtcacag gacaggacga ccgcccgact
     6901 tcggtacgca cggtccccta tcaaggcatt gcttctccct tcgaaaccgt gtaggtttcc
     6961 cgacgtcccg tccgtcatcc aaaaccgcag ttctgtcatc atcccagagc ttgtgaagga
     7021 gccattgatg gtcgactatc aggaattagc aaacaaagcc gtccgtgccg aactgctgaa
     7081 tcgttcggag tgtcagaccg tcttgaatac gccggatgac gacctgctcg cgcttctcca
     7141 ggctgccttc actgtccgtt cccgctactt tgggaaaact gttcgcctgc aaatgctgca
     7201 gaacgccaag agcggcgcgt gccagga
//
LOCUS       DATDNW010000336         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_593623, whole genome shotgun sequence.
ACCESSION   DATDNW010000336 DATDNW010000000
VERSION     DATDNW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593623"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..336
                     /locus_tag="VI359_08060"
                     /pseudo
     CDS             7..336
                     /locus_tag="VI359_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
     gene            325..771
                     /locus_tag="VI359_08065"
     CDS             325..771
                     /locus_tag="VI359_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HEY6974245.1"
                     /translation="MTSNVVYGKNTGNTPDGRRKGQPFAPGANPMHGRDKHGVHASAA
                     SVAKIPYRDAADGISLTTTLLPEGLGRIEEDRIKNLRGILDAFFGVTGYHMNVNVLNR
                     ETLLDAMDHPEKYPSLTIRVSGYAVNFVRLSREQQLDVINRTFHGS"
     gene            950..1795
                     /gene="pflA"
                     /locus_tag="VI359_08070"
     CDS             950..1795
                     /gene="pflA"
                     /locus_tag="VI359_08070"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HEY6974246.1"
                     /translation="MSSKEKDQYESLEAKSPFELRVNKGSKVSESVIKQALDTGDIGF
                     IHSFTTGSAVDGPGMRVVAWLSACQWRCLYCHNPDTWSLTNGTPVPLPHAIEELRKYR
                     HGLKVMSGGLTVSGGEPLMQDRFAIRLFAAAQEMGVHTALDTNGSLGARLSDDELKKI
                     DLVLLDIKTWDRERHRHLTGKDVGPTLDFARRLAARKRPCWLRFVLVPGLTDDPEDIS
                     QIAKFAAGLGNIERVDVLPFHQMGRFKWKELGLNYTLGNTELPSRELVQRVVGQFAAE
                     GLRAY"
     gene            1885..3030
                     /locus_tag="VI359_08075"
     CDS             1885..3030
                     /locus_tag="VI359_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY6974247.1"
                     /translation="MAVTESTVPLDRLSKRVAAIDWMRGFVMVLMVIDHASMAFDRDH
                     VDHDSAMYVDASTMALPGAEFFPRWLAHLCAASFVFLMGTSLAVSVERRVARGVNTWE
                     IDKGILVRGLIIALLDLTLISFGSGRWTFGVLMAIGLSMICMVLLRRLPTWGLLVTAL
                     GWIVLGEIVIGWVWTPPGNSSMLAAFTVANYSSPTIIFKYPVLPWLAITVLGWVFGRH
                     LIRFAGGQSTVSGKKVLWISGAVSLLIFAVVRGNDGYGNMFLHRADNSWQQWLHVSKY
                     PPSLTYYAMELGILFLCLALLRTIEERIGVRENGVFFVFGQTALFFYLVHRLVFEIPA
                     TYFGLRGFDGLAATYGATALMLLFLYPACRWYLTVKAAYPKSILKYL"
     gene            3235..3477
                     /locus_tag="VI359_08080"
     CDS             3235..3477
                     /locus_tag="VI359_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY6974248.1"
                     /translation="MNAQVTKRAKEKQPHTTMAGLPVAAVARGESARGPQPSFDDLYA
                     RIAVRAYELYVERGCRYGGALEDWLDAEREVLANLP"
     gene            3569..>3835
                     /locus_tag="VI359_08085"
     CDS             3569..>3835
                     /locus_tag="VI359_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974249.1"
                     /translation="MSYNKLNVLLLSLALGFSLQSSIASAGKSAEATQIDREADAALK
                     KLLEDTPEAEMFRKEAKGILVFPSIVKGGFIIGAHYGKGALKKDG"
BASE COUNT          833 a         1026 c         1132 g          844 t
ORIGIN      
        1 cactacatgc acgacaaata tgcgtatgag cggattgaga tggccctcca cgattatgcg
       61 ccggtgcgaa ccatggcgtt tggtatggcg ggtctatcgg tggttgccga cagcctctcc
      121 gccatccggt atgcaaaagt ccgcgtgatc cgtgatcaaa cgggcctcat taccgactat
      181 caaatcgaag gcgaatttcc tcaatttgga aataatgaca accgcgtcga ccagctcgcg
      241 acatgggtcg ttagcacatt catgaagaag ctgaggaaat atccgaccta tcggcatgcc
      301 ctgcacacac aatcaatcct gacaattacg tcaaatgttg tctacggcaa gaacaccgga
      361 aacactccgg atgggcggag gaaagggcag ccgttcgctc caggtgccaa tccgatgcac
      421 ggacgggaca agcacggcgt tcatgcgtca gctgcgtcgg tagccaagat tccataccgc
      481 gatgccgccg acggtatttc actcacgaca acgttgctgc cggagggtct tgggaggatc
      541 gaagaggata gaatcaagaa cctgcgcggc atactcgacg cgtttttcgg tgtaacgggg
      601 tatcacatga acgtgaatgt gctgaaccgt gaaaccctgc ttgatgcgat ggaccatccc
      661 gagaaatacc cgtctttgac gattcgggtc tcgggctatg ctgtgaactt cgtgcgtctg
      721 tctcgggagc aacaactgga tgtcatcaat cgtacgttcc atggctctta acttcatccg
      781 tggtgaaggg agagatttca tgaccgacgg ttcgctgttc ccaagcatgc atgatgtata
      841 cgggaactat tttaacaacc gtcgtttgac ccggcgaact gtcgttgtcg ccaatttcga
      901 cggaccggac cggatcgaaa tccgtgctac ggcgagaaag taggaagata tgtcttcgaa
      961 agaaaaagat cagtatgagt cgctcgaggc aaaaagcccg ttcgagctcc gggtgaacaa
     1021 gggcagcaaa gtctccgaat cggtcatcaa gcaagcactc gataccggag acatcggttt
     1081 cattcattcc tttacaacag gatcggctgt cgatgggccc ggaatgcgcg tcgtcgcatg
     1141 gctgtcggcc tgccagtggc gatgcctgta ttgccataat ccggacacgt ggagcctgac
     1201 aaatgggacg ccggtcccgc ttccacacgc gatagaggag cttcgaaaat atcgtcacgg
     1261 actgaaagtg atgtctggag gcctcacggt gagcggtggc gaaccgctga tgcaggatcg
     1321 gtttgcgatc agactatttg ctgccgccca ggagatgggg gttcacacgg cattagacac
     1381 gaacggttcc ctgggcgctc gcctcagcga cgatgagttg aaaaagatcg atctcgtgct
     1441 gcttgatatt aagacgtggg atcgggagcg ccatcgacac cttactggta aagacgtcgg
     1501 cccgacactt gatttcgcgc gtcggctggc tgcccgcaaa cggccatgtt ggctccgctt
     1561 cgtcctggtg ccgggattaa ccgacgaccc ggaagacatc tcgcaaatcg ccaagtttgc
     1621 ggctggtctg gggaatatcg agcgcgtcga tgtgctgcct tttcatcaga tgggccgctt
     1681 caaatggaag gaacttggtc ttaattacac tctaggaaat acagagctcc cttctcgtga
     1741 actggttcag cgcgtggtcg gtcaattcgc ggcggaaggc ctgagggcat actgattgcc
     1801 taagtagcat gacgctggaa ttcaaaatgg caggtcgatc cgcgcaggtt tgggggtccg
     1861 ggtgaggcct gatagggagc cgagatggcc gtaactgagt cgacagtccc attggatcga
     1921 ctctcgaaac gggttgccgc catcgactgg atgcgcggct tcgtcatggt tttgatggtc
     1981 attgaccacg cgtccatggc gttcgaccgc gaccacgtgg atcacgactc cgcgatgtat
     2041 gtggatgcaa gcacgatggc tctgccgggg gcggaattct ttcctcgctg gctcgctcac
     2101 ctttgcgcgg cctcgtttgt gtttctcatg ggtacttccc tcgccgtgag tgtggagcgg
     2161 cgcgtggcga gaggcgtcaa cacctgggag attgacaagg gcatcctggt tcgtggactg
     2221 atcatcgctc ttctcgatct cacgctcatc tcattcggtt ctggccgctg gaccttcggg
     2281 gtgctgatgg ccatcggcct ctcgatgatc tgcatggtac tgcttcggcg actgcccacc
     2341 tgggggttgc tggtgacggc cctcggctgg atcgtgctgg gagagatcgt gatcggctgg
     2401 gtttggacac cgcccggcaa ctcttcaatg ctggctgcgt tcacggtggc gaactattca
     2461 agccccacga tcatcttcaa gtacccagtg ttgccttggc tggccatcac ggtccttggc
     2521 tgggtattcg gccggcacct gatccgcttc gctggcgggc agagcacggt ttctggcaag
     2581 aaagtcctgt ggatttccgg tgcggtgagc ctgcttatct ttgccgtcgt ccgaggcaac
     2641 gacggatacg gcaacatgtt cctgcaccgc gcggacaact cgtggcaaca gtggctgcat
     2701 gtcagcaagt atccgccctc gctcacgtac tacgcgatgg agcttggtat cctgttcctg
     2761 tgcctggcgc tgctcagaac catcgaagag cgtatcggag tgcgcgagaa cggcgtgttc
     2821 ttcgtcttcg ggcagacggc cctgttcttc tacctcgtcc accggctcgt gtttgaaata
     2881 ccggcaacgt atttcggcct ccgtggcttc gacggcttgg ccgccaccta cggtgcgaca
     2941 gcactgatgc tcctgttcct atacccagca tgccgctggt atctcacggt gaaggcagcg
     3001 tacccaaaat cgatcttgaa gtatctgtag aaaatctgag gcgaagcgac ttttatctaa
     3061 ttggaaccaa gtcttcatca ccatcgatcg gatgagtcga gaggggacgc tcaaactgat
     3121 gcccaagggg cgaggtgtct atacagttca gtcttcacac cacgttcaac aggacacatg
     3181 tcactaggtc aatgcaatga agattgagtg actgtggtgc aagggaggac cataatgaat
     3241 gcacaggtga ccaaacgggc taaagagaag cagccccaca ccactatggc cggcctgcca
     3301 gtcgcagccg tggcaagggg cgagtccgca cgcggcccac aaccctcgtt tgacgattta
     3361 tacgctcgta ttgccgtgcg agcgtatgag ttgtatgtcg aacgaggttg ccgatatggt
     3421 ggtgcgctgg aggattggct ggacgccgag agagaggtat tggcgaacct gccatgagag
     3481 atggttctga tccacttcta cgaaccaaga ccacggtcgc tcgagctgac acctgctccg
     3541 tttcgacgga ccggagaggg aacaaaccat gagctacaac aagctgaacg tcctgctgct
     3601 ttctctggca ttggggttct cgctccagag ctcaatcgca tcggcaggaa aatcggctga
     3661 ggcgacgcag atcgatcgcg aggcggatgc ggcactcaag aaactgctcg aagatacacc
     3721 ggaagcggag atgttccgga aggaggccaa aggcatcctt gtcttcccga gcatcgtgaa
     3781 gggcggcttc atcatcgggg cccactatgg gaagggggcc ctcaaaaaag atggg
//
LOCUS       DATDNW010000337         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_467519, whole genome shotgun sequence.
ACCESSION   DATDNW010000337 DATDNW010000000
VERSION     DATDNW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467519"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VI359_08090"
     CDS             complement(<1..656)
                     /locus_tag="VI359_08090"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY6974250.1"
                     /translation="MNFWKTLPTPIIGLAPMDGVTDASFRHTVAIHGKPDITFTEFTH
                     VHDVCRGPEFLLESLFYSEVERPIVAQLYGKDPALFYQAAHVVCELGFDGLDINMGCP
                     SRNVASSGSGAGLIRTPDLAHEIMRAARQGITDWATGQSLESAGLKPARIAAIQRMNQ
                     QQSQPHPAIKRTIPLSVKTRLGYDAVIVDRWIEHLLAEHPAVISLHGRTLQQMYRGEA
                     "
     gene            complement(653..2059)
                     /locus_tag="VI359_08095"
     CDS             complement(653..2059)
                     /locus_tag="VI359_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="HEY6974251.1"
                     /translation="MDVYSPGLIELVDCLVNRRLLPDYLEIFKASTSALKFIRQHLSG
                     IALSYHGEGLWVTQPESRDHPTFDDAAAEAAAHLNVLQSPWLNHECATKQIAGYSFGT
                     YLPPLFTPVSAEVVAQNIIVVQAVLDARCCRPDGSTPLFLLEMPPLTYFAAGTIPITQ
                     FFRLVTERVPCGLVLDIGHLWTVYRYTGMWRRMSLVQFVQEFLDEFPTDRVVEIHVAG
                     LAAHVAIHAREIDSELPEWLDAHAAPIPPVLFEMLEQVLAHRSLTSLKGVALEVDTKP
                     IDLIVDEFEQVSRQFTPLIQEAMDREITMNLLSEQSPHDGYRSSIVSQFDRTQLCSDY
                     EQYAKIVSGLAVPVGDEWKAAAHDLEGLERYRTSYLPYEILEWGGALTDMFSETCRGL
                     RQCGIELEDFVAFWFREPRPITQAYDFFLLKIERFIEFVRERAPGLTACVEQEAACLR
                     AAYAEANEPVTPISRSAG"
     gene            2314..3072
                     /locus_tag="VI359_08100"
     CDS             2314..3072
                     /locus_tag="VI359_08100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY6974252.1"
                     /translation="MSTSDKYPPRTTNPTPTVVAHMMTPGVVQIPGDVSVSEAALMLE
                     REQTPCLLVKDTDTLFGLMTPTDIVKKVVAQGLEPHDIEARTIMTQPVQFIEYDRALE
                     EATALMTSTGASHLIVTKDNQPVGVLSARDLMLTPPPRRTRIPAMLSLSQNGSVAGTA
                     PHSATIIQLGHLGAQLESGTPLDAGANILLVFSLPGQHTPLTIQGTVLASKKDRVGNA
                     SNIVDIQFTNVATTDQTRIKAWALQTLPHPQDRS"
BASE COUNT          723 a          905 c          878 g          674 t
ORIGIN      
        1 tccgcttcgc cccgatacat ttgctgtagc gttcggccat ggagggaaat tacagctggg
       61 tgctcagcca gcagatgctc gatccatcgg tctacaatga cggcatcata cccgagacgg
      121 gttttgacag ataacgggat cgttcgcttg atggctgggt gaggctgcga ctgctgctgg
      181 ttcatccgtt gaatggcggc gattcgagcg ggcttgaggc cggcgctttc caggctctga
      241 ccagtggccc aatcggtgat gccttgtcgg gccgcccgca tgatctcatg ggccagatcc
      301 ggggtccgga tgagcccggc accagacccg gaggatgcaa cgttgcggga cgggcagccc
      361 atgttgatgt cgagaccgtc gaaccccagc tcacagacca cgtgagcagc ctgatagaac
      421 agggcgggat ccttgccata gagttgcgca acgatcggcc gttcgacttc gctgtagaac
      481 aaagactcca gcaaaaactc cggtcctcga cagacgtcgt ggacatgggt aaattcggtg
      541 aaggtgatgt ccggcttgcc gtgtatcgca accgtgtggc gaaacgaggc gtctgtcact
      601 ccgtccattg gtgccaggcc gatgatcggc gtgggaagtg ttttccaaaa attcatcccg
      661 cactcctcga gatcggcgtg accggctcgt tggcctcggc atacgcggca cgtaagcatg
      721 ccgcttcctg ctccacgcag gcagtcagcc cgggtgcccg ctctcgcaca aattcgataa
      781 atcgttcgat cttgagcaga aagaaatcat aggcctgggt aatcggccgt ggctctcgaa
      841 accagaatgc aacgaaatcc tcgagttcaa tcccgcattg gcgtaagccc cgacaggtct
      901 cggagaacat gtccgtgagc gcgccccccc actcaagaat ctcataaggt agataggacg
      961 tgcggtaccg ctcaagtccc tcgaggtcat gcgcggccgc tttccactcg tcccctactg
     1021 gaacagcgag ccctgacaca atcttggcat attgttcata gtcgctacat aactgagttc
     1081 gatcgaactg actgacgata gatgatcgat acccgtcgtg gggcgattgt tcggagagga
     1141 gattcattgt tatttctctg tccatcgcct cctgaatcag cggagtgaat tgccgtgaca
     1201 cctgttcgaa ctcgtcgaca atcaagtcga tcggtttggt gtcgacttcc aatgctacgc
     1261 ccttcagact cgtcaggctc ctgtgggcaa gcacttgttc cagcatttcg aaaaggaccg
     1321 gtggaatcgg agctgcatga gcatcgagcc attcgggcag ttcggaatcg atctcgcgcg
     1381 cgtgaattgc tacgtgtgcg gctaaacccg cgacatggat ctccacgacg cggtccgtcg
     1441 ggaattcatc gaggaattcc tggacgaatt ggaccagcga cattcgtctc cacattccgg
     1501 tgtagcgata caccgtccag agatggccta tatccagcac gaggccgcaa ggaacacgct
     1561 cagtaaccag ccggaaaaat tgcgtgatcg gaattgtgcc ggctgcgaag taggtcagcg
     1621 gcggcatttc taacagaaac aatggggtcg acccgtcagg tctgcaacat cgggcatcca
     1681 gcacagcctg caccacgata atgttttgag ccacgacttc ggcgctcact ggcgtgaaaa
     1741 gtggaggcaa gtaggtgccg aacgaatagc ctgctatctg cttcgtcgcg cattcatggt
     1801 tcagccaggg gctctggagt acattgagat gtgccgcagc ctcggccgcg gcgtcatcaa
     1861 acgtgggatg atcacggctt tctggctgag tcacccacag tccttctcca tgataggaga
     1921 gggcgatgcc cgataggtgc tgccttatga acttcagcgc gctcgtcgag gctttaaaaa
     1981 tctcgagata atccggcaag agccttcgat ttacgagaca gtccacgagt tcgatcaggc
     2041 cgggagaata gacgtccacc gagagtccaa ggccctgagg ggaaatcgct cgaagccgat
     2101 ccgcaaaagc tcggtttgca tttccgaaga cagtcaagaa tccacccttg tgctgtgcca
     2161 aaatttggcc tagtctagtc gccaaacccg ttgtttgaca agggaagacc ttgatcctga
     2221 ccaagagttc ccgtctgcta tacttggctt acgccgacgg gggcgaaacc ctcacgaacc
     2281 tgggaaaacc gcgacaggag cggtttcgaa cccatgtcaa catctgataa gtatccccct
     2341 cggaccacga atccaacacc gacggtggtt gcacatatga tgacaccagg ggtcgtacag
     2401 attccgggcg acgtttccgt gagtgaggcg gcgctcatgc tcgaacgcga acagacgccc
     2461 tgtcttctgg tgaaagacac cgacacactg tttggactca tgactccaac cgacatcgtt
     2521 aagaaagtgg tcgcacaggg gcttgagccc catgatattg aggctcgtac gattatgacg
     2581 caaccggtcc agttcatcga atacgaccgt gcgttggaag aggccaccgc actcatgacg
     2641 tcgacgggag cctctcattt gatcgtcaca aaagacaacc agccggtcgg cgttcttagc
     2701 gcacgcgatc tcatgttgac tccgccgccg cgtcgcactc ggattcccgc gatgctcagc
     2761 ctgtcacaaa acgggagcgt ggcagggacg gctccgcaca gtgccaccat tatccaactc
     2821 ggccatcttg gggcgcagct tgagtcaggc actccgttgg atgctggagc gaacatcctc
     2881 ctcgtatttt cgttgcccgg tcagcacaca cccttgacga ttcagggaac ggtgcttgcc
     2941 agcaagaaag accgtgtggg caacgcttcg aacatcgtcg atatccaatt cacaaatgtg
     3001 gcgacgacgg accagacacg tatcaaagct tgggcattgc aaacacttcc gcatcctcaa
     3061 gatcgctcct gactggtctc ctccattgct ttgaccaatc atgaatcagc tgatagaatt
     3121 ttgtctctac cgcgaaaccg cgtatactgc tgtgatgaat gatccgatga aatccacgcc
//
LOCUS       DATDNW010000338         4840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_930296, whole genome shotgun sequence.
ACCESSION   DATDNW010000338 DATDNW010000000
VERSION     DATDNW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930296"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..919)
                     /locus_tag="VI359_08105"
     CDS             complement(323..919)
                     /locus_tag="VI359_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6974253.1"
                     /translation="MNMRGFWLGLAVGTLSTGLLGQWVFSAASKETDPPKCISPEIVA
                     DYIHSVVQADRTFYTSEIVERMRARGIVSSSEHWKEDGDLPLPAQFVLETGRLVAKQS
                     NGIRYRLISNWPINRRNSPTTEFERTALTKILVNTDRPYTGVTTEGRARVFQALYPDK
                     AVSQICADCHNVHANSPKRDFKAGDVMGGILLTIPLSQ"
     gene            complement(1090..1404)
                     /locus_tag="VI359_08110"
     CDS             complement(1090..1404)
                     /locus_tag="VI359_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974254.1"
                     /translation="MKTLMIVARDSRMHELEEVLHDNGVDAYSLLNKVMGKGRTGTVY
                     ESLFHTGYTGFNLMILAVLPSDHAERVVSALKAFHAARVKGAHGEPIAFKLFSFPCEE
                     LI"
     gene            complement(1846..2637)
                     /gene="modA"
                     /locus_tag="VI359_08115"
     CDS             complement(1846..2637)
                     /gene="modA"
                     /locus_tag="VI359_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY6974255.1"
                     /translation="MAGIQKVSIAMALVCGLASVVATGERVQAETLTVAAAHSLKGAF
                     QDIVPMFEKEYGVTVQVVYGPSQTLRRQIEQGAPIDVFLPESFEEVEKLRKKGLTLNG
                     GPRIFAQTSLVLVMATTSPATSISFREVYPDRAIRIALVDPKTSALGEITAKELTKLD
                     PAYINRSHLLHAQHSDDVLNLLRRGEADVGIVYRADAINSGQVRIIDEAPAGRYTPVQ
                     FGEAVVWTCRKESFNAAEGFFDFIMSPRVQKLLLQYGFDSVPSNG"
     gene            4645..>4840
                     /locus_tag="VI359_08120"
     CDS             4645..>4840
                     /locus_tag="VI359_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6974256.1"
                     /translation="MNQIVLIALRRPYTFVVMSILIVLLGSLTVLHMPTDVFPNITIP
                     VTSVVWVYAGLLPQQVEGRIT"
BASE COUNT         1101 a         1307 c         1223 g         1209 t
ORIGIN      
        1 tctattgagc ttcgaatcgg aaccctgcac ataagcaggt tctacagatc tttcctaccg
       61 cttcgctgtg tctataatgg cgagtcgatc cgcttttggc tgctgcgtca cccattcgac
      121 gacacggagt caaatccata cttgagcagg agtttttgga tgcggggact catgatgaaa
      181 tcgaaaaact cttcagcggc agtaagggat tcgttccgac aggtccacac caccgcttgc
      241 ccgaaccgtc aacgtttcag gctgcgcacg gtctgcggtc gcaataagtg ccgcctgaac
      301 agctcatttc agttggcttt ccttattgag agagtggaat cgttagaaga atcccaccca
      361 tcacgtctcc cgccttgaaa tctctctttg ggctattggc gtgcacattg tgacaatcgg
      421 cgcatatctg agacacggct ttatcgggat agagtgcttg gaagacacga gccctaccct
      481 cggttgtcac gcctgtatag ggacgatcgg tattcacaag gatcttcgta agcgcggtcc
      541 gctcgaactc tgtcgttggg ctattccttc tatttatcgg ccaattgctg atcagccggt
      601 atcgaatccc atttgactgt ttagccacga gccgccccgt ttcgagtaca aattgagcgg
      661 gtagagggag atctccgtct tctttccagt gttccgagga ggagacaatg cctcgcgccc
      721 gcatccgctc cacaatctcg ctcgtgtaaa aagttcgatc cgcctggacc acactatgta
      781 tataatcggc gacaatttcg ggggagatgc acttgggcgg atctgtctcc ttgctcgcgg
      841 cggagaacac ccactgaccg aggaggccag tggagagggt tccaacagcg agtcccaacc
      901 aaaagcctct catgttcatg ctgcatcctc catttctttt tctgcctgtg gcttgattcg
      961 ctcattggct ccgatagctg cacggtcaaa ccaatcatgc cgaacagttt ctgagctagt
     1021 cctattgaac cactcttaag gctgagaaac cctcgctatg aagacagtgg gcggttgtag
     1081 cccatggcgc tatatgagtt cttcacaagg gaaagaaaac agcttaaagg cgatgggctc
     1141 cccgtgtgcg cccttaactc gtgctgcgtg aaacgccttg agcgcgctga ctaccctctc
     1201 tgcgtgatcg gatgggagaa cagccaggat catgagattg aagccagtgt agccggtgtg
     1261 gaagagggac tcatacactg taccagtcct gcccttaccc attactttat taagtagtga
     1321 gtacgcatcg acgccattat cgtgcaagac ctcctccaac tcatgcatcc tagagtcccg
     1381 agccacaatc atcagcgttt tcatgtgtga accatgcctc ctcctgatcc atttgttttt
     1441 tggactgctg gaaggatacg cgtatctcct attaaaatcg aggtgctacg aggggatggc
     1501 ttctagagtt tttgactgac ccttcttctg gtgcggctgg gggtaaattg tggcaggaca
     1561 attttgcccg atcaggttgg accgaatcat tcactatcac ccgcaggttg cccgctcaat
     1621 ccgggtcgct ggctccacaa tactccaagc aaattccgga agtttgatat ggctcactcg
     1681 ccaatctcta gacggggaat cagctggagc agaaggagac tgtggttggt cgtgacggaa
     1741 aatcattggg tgggggtcgg cgtcgttcag tcggcacggc gccgaacgag cgctaaggcg
     1801 gcaagacctg agtaatctag ataactaaga aggccagcga gtaagtcacc cattcgatgg
     1861 cacggaatca aatccatatt ggagcagaag tttttgtacg cggggactca tgatgaaatc
     1921 gaaaaaccct tccgcggcat taaaagattc cttccgacag gtccacacca ccgcttcccc
     1981 gaattggact ggcgtatacc ttccggccgg ggcttcatca atgatgcgca cttgcccgct
     2041 gttgatcgca tccgctcggt agacgatgcc cacgtcggcc tctcccctgc gaagcagatt
     2101 cagtacatca tcactatgct gcgcgtggag aaggtgggaa cggtttatat aggcgggatc
     2161 gagctttgtg agttccttcg ctgtgatttc tcccaacgca gatgtcttag gatcgaccag
     2221 agcgatgcgg attgctcgat ccggatacac ttcgcgaaag gagatcgatg ttgccgggga
     2281 ggtcgttgcc atgacgagca cgagagaagt ctgcgcgaag atgcgaggtc ctccgttgag
     2341 agtcagacct ttcttacgca atttctcgac ttcttcaaac gattcaggaa gaaacacatc
     2401 aatgggggct ccctgttcaa tttgccggcg aagcgtctgc gacggaccgt acacgacctg
     2461 taccgtcaca ccatattctt tctcgaacat cggcacgata tcttggaatg cacccttcaa
     2521 actatgggcc gccgcaaccg tcagggtttc agcttgaact cgctcgccgg tcgcaacgac
     2581 gcttgcgagt ccacatacga gagccatcgc gatactgact ttctgtatgc ctgccatgat
     2641 acgtcacttc ctgaccccga ctcatcaggg ggtcttacag ggtgttgccg atggacctga
     2701 tccttgaccc tgaatcgatt ctctgccagt tgccgaacat acgagaaccc acgatcacag
     2761 tgggggtacc agcaagatca ataccaattg tagtattcaa tcgacactct gaggaaaacc
     2821 agtccaaccg ttggattttt aacaattctc tcatcactct ctgccgaacc gcatagtctg
     2881 tacctcctca gaattgtgag attccgcacg attcgctcag tgcgaaaagg gcttgaagat
     2941 caggctcctc gacgacgctt tgaccttcgt gaaggattcg atctcggatg tcctgcatga
     3001 aatgctaacc gccggtgcgc tggtcggcct catcgtgctg ttgctgctcg gctcatggtg
     3061 ggccacggtc atcgtctgga cctccatcct gttgtccatt ctgactgcca ttgctgggcg
     3121 gagtagccgt cgcctacttg tcgttcactc gtctcttcga gcctcatcag attcagtacc
     3181 ttgcagcagt ggaagccgcc tcgattctcg ggccttgaaa gttttcctga tgctatgacc
     3241 aatactgcta tgggagcgcg tatgtggacg aaggtaataa cgtgaccttg attgacactc
     3301 gttctatgca gtcgagcgtc ttagctcaac gcgcacagtg cgaaatggta aggaaactct
     3361 agagttcggc cggcactatc caatcttatc tgcgatacca ttacaatcct cttgtttttt
     3421 tagatcttct gataggcagt ttcagaaggt ctagccagcc ctcttagggg aataacattt
     3481 gcctgttttc ttggttctca gaaggacagt gcggtgatgg gatagtgaag aataaggtta
     3541 tcatctctac ttagcgcacc tttacccacg ccgccttcat agagggctcc ggccatgcaa
     3601 caccagaaga ctggcccaac cagttgggac tcatcggcag cggcgatcgg ctaagggctg
     3661 ctaacctgat cgagtttcgg ctaacggact cctgcggtag agagagtatc cacatggctt
     3721 atcccgccag agaaatatct aatgaggaaa tcgagaacgc gaagaccggc aaggcgctga
     3781 aagaattctt ccggcgagag cgccaatccc aagaagaccg cgcctcaata tggtgggtca
     3841 gcgtcgggat ggtcatggtc tttctcattc taggcttgct cctgatccaa gttttattcg
     3901 tggcgccgaa aaacagacat ccaactgaaa gcgcttggta ctgccacgct ttgcccccct
     3961 tcaaaattct cagtcgagac cgtcctggtt ccaatcttcc acggaactgc cacactgccg
     4021 tcctgccata aacatcaagg atattgatac atcgtttgca acgctgagcc tgctgattcg
     4081 gagatcccac caagcagttg tgcccttcgt gtgcaggacg aatgatgggg ccatgcatgg
     4141 ctttcatgca aagtgatctg gcgaggagtt tctttctcag cgaatcctct ttcgttcctt
     4201 gcagacagct caaccgatcg tgggtgaaat cgtgagattc cgcacgatcc gctcagtgcg
     4261 aaaatggggg aatccgcaat cttcatgctt ttctgttcga aacacgagac agaactgccg
     4321 acgatattgt gccgataaat tgcggtcagc ctgcttgatt ggtttatctc agccatctgg
     4381 ttggtttcgt tcataaaacc aagaaacccc ttgctatggt ggggaattta caggctgtgg
     4441 agatccctag catttcccac cattgcattc ctcagcaatg tgcattgcgg cgtgagagaa
     4501 gtagactacc tgctcgtacc atatagagcg aatacgacga gtggtatcca ctttgctata
     4561 cctacggatc gtgttgatct agggcctact ttaggtagat gatcatatga caaaaaaatc
     4621 aaccgctctt ggcacacttc gcccatgaac cagattgtcc ttattgccct ccgcaggccc
     4681 tacaccttcg tcgtgatgtc catcctcatc gtgttgttgg ggagcctcac ggtgctccac
     4741 atgccaaccg acgtcttccc gaatattacg attcccgtca cctccgtagt ctgggtttac
     4801 gccggcctgc tgccgcagca ggtggaaggg cgcatcacgt
//
LOCUS       DATDNW010000339         9020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_467912, whole genome shotgun sequence.
ACCESSION   DATDNW010000339 DATDNW010000000
VERSION     DATDNW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467912"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..924)
                     /locus_tag="VI359_08125"
     CDS             complement(280..924)
                     /locus_tag="VI359_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974257.1"
                     /translation="MSMRSKQWKLSLAGIVVGLLGTSVLESTAASLDQLTDLTGRATA
                     VVTLKGRDNFTSEYRYDVSVKNQSPDLLIADSLVIVLDKITNLAGEEQDNLKGESFLK
                     LFDVLGQDGETDDGKPFFRIPIGSSQDLAPQTLSLPAAVRIRNKEYLIVFTPSFKVFG
                     QKRVPPEPRRIEATPPAPVPTPAPPSRNSVDKLIQLLIKKGVLTEEEWHKANQP"
     gene            1128..1997
                     /locus_tag="VI359_08130"
     CDS             1128..1997
                     /locus_tag="VI359_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974258.1"
                     /translation="MLSSAVRRAPLLLPGSLLSKLMRLYDYPHPMRRGRLIRGYDRPH
                     AVRTARMCAAVATALGHEAERVRLYQIACLLHDLGRAGLDRVLFSRIWSWAKERGIPT
                     RPREWRAIHPETKYGRETEAFVARYRKALEADGIRMDAWAKEQVEMRLGYARRLARRL
                     RTARPSMHRMGIRWAPWMQQVMLYYYYPERLAAAQPWVKQLAEILVACEQFEAYSNRR
                     RGRDYYVRKTETLTEAFTYLEKLQHEGILSEPVMKTLRKLVAQGTFDAILEEARGQAF
                     TAIERRFLRSMEY"
     gene            1999..2424
                     /locus_tag="VI359_08135"
     CDS             1999..2424
                     /locus_tag="VI359_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY6974259.1"
                     /translation="MAVKTIPLRINMRGDTQIENITKTVGEAVAGTKLSAGIVTVFVR
                     HTTASIMIIEDEPGIRADTKTFWERVVPADPHWQHNTRNAGEDNGHSHLRGQLQGPSI
                     TIPFVDGAPLLGTWQQIVIVDFDTRARVRDLVVQVIGES"
     gene            2421..3557
                     /locus_tag="VI359_08140"
     CDS             2421..3557
                     /locus_tag="VI359_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY6974260.1"
                     /translation="MTRMFAQVELIFGLSILFWFGWFTSNVVPAELGKPLGSTYDGPE
                     GRPRPVTPAPTELSPDERSTMAVFERATKSVVFIANSAVRRDPWSFDLFEVPQGSGSG
                     FIWNKQGHLVTNFHVVYGATSITVTLADRTEHKAKVVGADPDHDLAVLQIQAQEESLS
                     PVVIGTSHDLRVGQKVLAIGNPFGLDHTLTTGVVSALGRTIKSLSNRTIEGVIQTDAA
                     INPGNSGGPLLDSAGRLIGVNTQIVSPSGAFAGIGFAVPIDTVNRIVPELIKYGKLIR
                     PGLGVSLVPDAIARRWGIKGVVIGKVVRGSGAERAGLRGARETVTGRVELGDIIVGID
                     GKPIETIDDMLDLLEQRKVGDQVMVEILRANRRQQVSIALQAVN"
     gene            3784..4242
                     /locus_tag="VI359_08145"
     CDS             3784..4242
                     /locus_tag="VI359_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974261.1"
                     /translation="MKARTWMMEYGVAIILTALFAVVLGRIPLFRETAVGNLHASDLV
                     QFIGYAGAMVIAWFGARQLSAEPPDDWKWLMPYRALILPVVTLAMVAIAYGVLLYVCE
                     PFMSKSGKTVYNWFFIAGIVAASVWLIMTWVMKCAPQVAAMESRRLRRAA"
     gene            complement(4291..5274)
                     /locus_tag="VI359_08150"
     CDS             complement(4291..5274)
                     /locus_tag="VI359_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY6974262.1"
                     /translation="MGPITPFEPPRILRNTHAMTLFPQYWPRGMQLMGVPQEDRLFTV
                     EPGTQLLGRCHWQRDRKSTATLILVHGLEGCSESHYMHGIAAKAYRSGLNVVRMNQRT
                     CGGTEHLTPTLYNSGLSGDYRSIVRELTTVDGLDRIWLIGYSMGGNLVLKAAGEFGSS
                     EPTLKGVVTVCPNIDPTQCVDALEHPSNWIYHNHFLTRLKARLRRKAILIPGKWDLRG
                     LARIRLIREFDDRYTAPDGGYRNGADYYDRAGSRHVLGSIAVPTLIITAQDDPFIPYS
                     MFALQAIEQNPSITLVAPRYGGHCGFFQWKRNGEDAYWAENRIVEFVTRGV"
     gene            complement(5337..5412)
                     /locus_tag="VI359_08155"
     tRNA            complement(5337..5412)
                     /locus_tag="VI359_08155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5377..5379),aa:Asn,seq:gtt)
     gene            complement(5512..5781)
                     /locus_tag="VI359_08160"
     CDS             complement(5512..5781)
                     /locus_tag="VI359_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974263.1"
                     /translation="MPNEDNVIDELLGEIAGLIHEYPKAIERRAAEIQAAGKDPELVE
                     KLVKAADTMRDSGNLYITWAKHFAALAEGNTDASSDEDETEDFDV"
     gene            complement(5868..6146)
                     /locus_tag="VI359_08165"
     CDS             complement(5868..6146)
                     /locus_tag="VI359_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY6974264.1"
                     /translation="MYNVAQVIDEKCTAKKGCRLCIMYCPEANCLDLNVTKMVAEVNI
                     DRCKGCELCVVVCNAAKHQAIVMQAVSASGELMSRKGESAALGQAYQG"
     gene            complement(6170..6877)
                     /locus_tag="VI359_08170"
     CDS             complement(6170..6877)
                     /locus_tag="VI359_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY6974265.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVLAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIQVFHPQVITMGKSYTMPFYSGVKEGGVV
                     IINSAQSLLSEEDIQRLKDLNVAVFYIAGTELAIEVAGTELSTNMTMIGSVAGITKCV
                     SMDALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     AEKNKIELRVGNPAVAA"
     gene            complement(6902..7807)
                     /locus_tag="VI359_08175"
     CDS             complement(6902..7807)
                     /locus_tag="VI359_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY6974266.1"
                     /translation="MSKERIKISDALYDIMPSDYQDLVKSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALTVRFPGRTKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     VRNAVMVAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYISEPAK
                     QLLAEMAAKEKERKAAAKQLTSQEA"
     gene            complement(7865..>9020)
                     /locus_tag="VI359_08180"
     CDS             complement(7865..>9020)
                     /locus_tag="VI359_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6974267.1"
                     /translation="DTWQMMNDAPRTPSFYTGSEVIKEALRRASCDVMIAYPITPQSE
                     AAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGARVFTTTAGPGTMRAMENFPM
                     WAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGMLVWHAETAQDFFDWILKGYM
                     VSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPYDPYRSPVPCMDMECPPVRM
                     MRDPFIMKSNYISYATHASWQQEVWAAVERSRKHSIHWLNGLIDTENTDADIMIVASG
                     TAVSQGREAIRLLEDEGIRCGLVKVKTLRPWPEEEIREATKNAKHIFVPEFNVTGWLA
                     KEIRASIPNHQRVHAGPHVCGGMTMPPEIIVSEIKTALGMRTMSLAGRGS"
BASE COUNT         1862 a         2474 c         2598 g         2086 t
ORIGIN      
        1 tggggaacct gtgaccctcc ggtctctcta ttcgttagtg agcattatac caccgggtag
       61 gcggaggaag agtctgagcc tgctcagggt ttcatccctt cgacaattcg caaagtcagc
      121 atctggggcg gattaggaac cagttgtatg gagcatacag gccacgatgg cctgctcggg
      181 agggaatttg tcatcataaa gatagacctt cgagaggtcg gggttggcaa tgaggggggc
      241 tggatcagct tcccgcaccc gcgtgacagg ccgcgtcggt tatggctggt tggctttgtg
      301 ccattcttct tcagtcaaca cgcctttctt gatcaacagc tgaatcagtt tgtcgactga
      361 gtttcggctg ggaggggctg gagtcggcac gggggctggg ggagtcgcct cgatgcgacg
      421 cggttccggt gggacgcgtt tctgtccgaa taccttaaac gagggcgtga agacgatgag
      481 gtattctttg ttcctgatgc gaacagcagc agggagactg agggtctgag gagcaagatc
      541 ctggctggat cctatgggaa tccggaagaa cggtttgccg tcatccgttt caccatcttg
      601 ccccaagacg tcgaacaatt tcaaaaaaga ttcacccttc agattgtctt gttcttctcc
      661 ggccaggttg gtgatcttat ccaggacgat aacgagagaa tcagcgatga gcaaatcagg
      721 ggactggttc ttgacactga cgtcgtaacg atactcgctg gtgaaattgt cgcgtccctt
      781 gagtgtgacg actgccgttg cccttccagt taggtcggta agctgatcaa gcgatgcggc
      841 ggtgctctcg agaaccgacg tccccagtag cccaacgacg atgcctgcca gactcagctt
      901 ccactgcttg cttcgcatcg acatggtcac ctcgctggat cgagaacact gccgcgtgct
      961 caaccgacct gagcatagca aagatcgtcg gtttgagcga gcgtttgtcg gccagcttgc
     1021 cttgacaggc tctttcaacg aacgatattc tgcgtgagtg cgctgcttcg cgcgctccga
     1081 gtcgtctgtt gagagcatcc cgtaaacctg agtgcggcac actgtccatg ctttcttccg
     1141 cagtccgacg cgctccattg ctgcttccag ggtcccttct ttctaaactc atgcggctct
     1201 acgactaccc gcatcccatg cggcgaggcc gtctcattcg cggctacgac cgaccccacg
     1261 cagtgcggac tgcacgcatg tgtgcggcag tcgctacggc gctggggcat gaggctgagc
     1321 gggtgcggct ttatcagatt gcctgtttgc tacatgatct cggacgggcc ggtctcgatc
     1381 gtgtgctctt cagtcggatc tggtcttggg cgaaggaacg gggaattccg accaggccgc
     1441 gcgaatggag agccatacat ccagagacca agtacgggcg ggagaccgag gcgtttgtgg
     1501 cgcgatatcg caaagctctt gaagcggatg gaattcggat ggatgcctgg gcgaaggagc
     1561 aggttgaaat gcggttggga tatgcgcgtc gtttggcgcg acgactccgg actgcccgtc
     1621 caagtatgca tcggatgggt attcgatggg ctccctggat gcagcaggta atgttgtact
     1681 actactaccc tgagcggttg gcagccgcac aaccgtgggt caagcaattg gcggagattc
     1741 tcgttgcctg cgagcaattc gaagcctaca gtaatcggcg ccgcgggcgg gactactatg
     1801 taaggaaaac agaaacgctt accgaggcct ttacgtatct ggaaaagttg cagcacgagg
     1861 gcattttgag tgagccggtg atgaagacgc tacgcaagct tgttgcgcag ggtacgttcg
     1921 atgccattct ggaggaggcc cgtggacagg cctttacggc tatcgagcgg cggtttcttc
     1981 gaagcatgga gtactgacat ggctgtgaaa accattccct tacgaatcaa catgcgtggc
     2041 gacacccaga ttgagaacat caccaaaaca gttggagaag ctgtggcagg cacgaagctg
     2101 tccgcgggca tcgtgacagt ctttgtcagg cataccaccg cctcgatcat gattatcgag
     2161 gatgaaccgg gcatccgcgc agacaccaaa acattctggg agcgcgtcgt ccctgccgat
     2221 cctcattggc aacacaacac gagaaatgcc ggtgaagaca acggtcacag tcatttgcgt
     2281 ggacaattgc aggggccgtc gatcacgatc ccctttgtcg acggggcacc tctgctggga
     2341 acatggcaac agatcgtcat cgtcgatttc gatacgcgtg cccgcgtgcg ggatctcgtg
     2401 gttcaggtga tcggcgaatc atgacccgga tgtttgcgca ggtggaactt atattcggac
     2461 tctctattct gttctggttc ggctggttca caagtaatgt tgtcccggct gaactgggca
     2521 agccgctcgg ttccacttat gatggacccg aaggacgccc gcgacctgtc acgccagctc
     2581 ccacggagtt gagcccagac gaacgatcga cgatggcggt gttcgagcgg gcgaccaagt
     2641 ccgtagtgtt tattgccaac agcgctgttc gacgcgatcc ttggtcattc gatttgtttg
     2701 aggttcctca gggatccggt tcagggttta tctggaacaa gcaaggccat ctcgtcacca
     2761 atttccacgt ggtgtatggg gccacgtcga ttaccgtcac gctagctgat cggacggaac
     2821 ataaggcaaa agtcgttggc gcggatccag accatgatct ggccgtactt caaattcagg
     2881 cgcaggaaga atcgttgtcg cctgtggtaa tcggaacgtc gcacgatctg cgtgtcgggc
     2941 aaaaagtatt ggccatcggc aacccgttcg gcctcgacca tacgctgacg accggcgtcg
     3001 taagtgcctt gggtcgcacg atcaaatctc tctcgaatcg gacgatcgag ggagtgattc
     3061 agacagatgc ggcgattaat cccggcaact ccggtgggcc gttgcttgat agtgctggcc
     3121 gcctgatcgg ggtcaatacg cagattgtca gtccaagcgg ggctttcgcg ggaatcggat
     3181 ttgccgtacc aatcgatacc gtcaatcgga tcgtaccgga gctgatcaag tatgggaagc
     3241 tcatccgtcc ggggctggga gtctcgcttg ttcccgatgc gatcgccagg cgatggggaa
     3301 ttaagggtgt cgtcattggg aaggtcgtgc gcgggagtgg cgcagagcga gccgggctcc
     3361 gcggggcgcg cgaaacagtt acgggccgtg tcgaactcgg tgatatcatt gtcgggatcg
     3421 acggcaagcc gatcgagacc atcgatgaca tgctggatct tttggaacaa cgcaaagtcg
     3481 gcgatcaggt catggttgag atacttcgtg cgaatcgtcg gcaacaagtt tcgatcgcgc
     3541 tacaagccgt caattaagag cgactccttc caccgtttct aaagaatata atcccttctt
     3601 gatgggcctc ttccctctcg tccgctccct tcatctgtct cggtatgaga tcgcgcttgt
     3661 tccgtcggta tcgtccagga agtaattcgt aactatctga atgtaaaaga caataaacca
     3721 ggtgctacac ttctgtgaag gctaaattca agaggtcact cagctgatct cgggaggtta
     3781 ctcatgaaag cacgaacgtg gatgatggaa tatggcgtgg cgataatcct gacagccctg
     3841 ttcgcggtgg ttctggggcg gatccccctg ttccgcgaaa cagcagtcgg caatttgcac
     3901 gcgtcggact tagtccagtt catcgggtat gccggtgcaa tggtcatcgc ctggtttgga
     3961 gcacgtcaat tgtcggctga accgccagat gactggaaat ggctcatgcc ataccgggct
     4021 ctgatactcc cggtcgtcac cctcgcgatg gtggcgatcg catatggcgt gttgctctat
     4081 gtctgtgaac cattcatgag caaatcggga aagacggtct ataactggtt ctttattgcc
     4141 ggcattgtcg cggcaagtgt gtggctgatc atgacgtggg tcatgaagtg cgcgccgcaa
     4201 gtggcagcca tggaatcacg gagattgagg agagctgcgt aacagtcgat cggtctacgg
     4261 ctgcctcggg caccgaggca gccgcctctc tcagacgccc cttgtgacaa actctacaat
     4321 tcggttctca gcccagtaag catcttcacc gtttcgtttc cattggaaaa atccacagtg
     4381 acctccgtag cgaggagcca cgagcgtgat cgacggattc tgttcaatgg cttgcagagc
     4441 aaacatggaa tagggaatga acgggtcgtc ctgggccgtg atgatcaacg tcggcacggc
     4501 aattgacccg agcacatgcc gcgagccggc tcggtcatag tagtcggctc cgttacggta
     4561 gccgccgtcg ggtgcggtat agcgatcatc gaactcgcga atcagtcgaa tccgagcaag
     4621 tccccggaga tcccacttac ccggaatcag tatcgctttg cgacggagac gagctttcaa
     4681 tctagtcaaa aaatggttgt ggtagatcca attgctcggg tgctccaggg catcgacgca
     4741 ctgcgtagga tcgatgttgg gacagaccgt aaccacgcct ttcaaggtcg gttccgacga
     4801 gccgaattct ccggccgctt tgagcacgag attccctccc atcgaataac caatcaacca
     4861 gatacggtcg agtccatcga cggtcgtcag ttcacggaca atcgagcgat agtctccact
     4921 gaggccactg ttataaagcg tgggggtcag gtgttccgtc ccgccgcaag tacgctggtt
     4981 catgcgaacc acattcagtc ctgatcgata cgccttggcg gcgattccat gcatgtaatg
     5041 cgattcgctg catccttcga gaccatgcac caggattagg gtcgcagtcg atttgcgatc
     5101 ccgttgccag tggcagcgac ccaacaactg cgtgcctggc tcgacggtga acagtcgatc
     5161 ttcctgaggg acgcccatca attgcatgcc tcttggccaa tactgtggga agagtgtcat
     5221 ggcgtgggta ttgcggagaa tccgtggcgg ttcgaatggg gtgatagggc ccatgtacac
     5281 atcctaaaat aaaaagggtg ccgttcctac tatgaacgac accctttttg tagatatggc
     5341 tccccgggca ggactcgaac ctgcgaccag ccggttaaca gccggctgct ctaccaactg
     5401 agctaccggg gaacatgcga cgtgttcagc tcaacgagag aaacatttta acaaaagtgg
     5461 cttgctcaag gggaatgttt ttttggccgg ttagttgagt gaaagatcac attagacgtc
     5521 gaagtcttca gtttcgtctt cgtccgagga ggcgtcggtg ttgccttctg ccagtgctgc
     5581 gaagtgcttg gcccaggtga tatagaggtt cccgctgtcg cgcattgtat cggcggcttt
     5641 cacaagcttc tccaccaact cggggtcttt cccggcggcc tgaatctccg cggcacgacg
     5701 ttcgatggct ttggggtatt cgtgaatcag accggcgatt tctcccagta gctcatcgat
     5761 gacgttatct tcatttggca tggtcttcct caaccgcgga gccaacaaaa aaccccatag
     5821 cgcgggcgcc atggggtctt gtgcggaagc cccgcttaca tgaacagtta cccttggtag
     5881 gcttggccta gcgcagccga ctcacctttg cgactcatca attccccgct tgcgctgaca
     5941 gcctgcataa cgatcgcctg gtgcttggct gcattacaga cgactacaca gagttcgcag
     6001 cccttgcagc gatcaatgtt tacttctgcg accatcttgg tgacattgag atccaggcag
     6061 ttggcctccg gacagtacat gatgcaaagg cggcaaccct tcttggcggt acacttctcg
     6121 tcgatcactt gcgctacatt atacatgcag aatagttcct tctcgcttgt tacgccgcga
     6181 cggccggatt gccgactcgc aactcaatct tattcttctc ggcccattcg ctggctattt
     6241 cgtaggcccg cttcacagtt gccagattct ttgctaaaag catttctttc ttggcgaact
     6301 ttttcttaat ggcttcgtct aaggaagccg taccaccgga ggccacgaat ttctttccga
     6361 agcgttcctg gagcgcgccg tcgagcgcgt ccattgaaac gcatttggta atgccggcca
     6421 cggaaccaat catggtcatg ttggtggaca actcggtacc ggcgacttcg atcgcgagct
     6481 cagttccagc aatgtaaaac acggcgacat tcaagtcctt gaggcgctga atgtcttctt
     6541 ccgacagaag agactgggcg gagttgatga taaccacgcc accttccttc acgcccgagt
     6601 agaagggcat ggtgtagctt tttcccatcg taatcacttg agggtgaaag acctggatga
     6661 cgtcagggaa tactaactcg cctcggtcgt agatccgttc gatgccgatg cgacagtagc
     6721 tttcggccgg cgccatccgc ttttcagcgc cgaagaacgg attcgagatt gaaaacttcc
     6781 cgtcgcggtt ggctgccatc gccaacacgt gtgcggctgt gacggcgcct tgcccgccga
     6841 gtcccgacat ccggatgttc agtctcttct tgatcatggt cgtctctcct ccagattcag
     6901 actaggcttc ctgtgaggtg agttgcttag cggctgcttt gcgttccttc tctttcgcgg
     6961 ccatttcggc caagagctgc ttggccggtt cgctgatgta ttccttgtac gcaaaccgct
     7021 cggtcggctt ctctgagtcg cgcatttctt ggaggccttc catgctgttc ttgccgattt
     7081 caagaatgca gggggtgtac aactgcaagt aggtggggcc gatctcccgc gcgaccatca
     7141 cggcattgcg aacgaccttt tcaacgagcg acggcttgct gaccgtgcag ttgacgacat
     7201 agtggcagcc ggattcccga gcaatctccg ggagacgaac cttgtcgaac agcttgccaa
     7261 ccggcgccat cttggcgacg aagcccttct gcatcaaacc gctctcttga ccgccggtgt
     7321 tagcgtagag ctcgttgtca aagcagatcg tggtgaactt ctcctggcgg aaccaggctt
     7381 gcagggtcat atcgagaccg atatcgacgg tcgcgccatc accagccagg accacaacgt
     7441 ctttcgtacg tccggggaag cgaaccgtca acgcgcgctt caggcccgag gcaattgcgt
     7501 tctgattgcc gaacaacgag tgaatgttgt gaacggctac catcgggaaa accaggctgg
     7561 tgcacccagt ggagcccacc atgaccgtat cttccgggtt cgggagtgag gccagaatgt
     7621 agcggaaggc catcgattcc ggacaaccgg cgcatagaga atgttgttcg atcagctcct
     7681 ttgaggtgcc gatgtccttc caccctcgat cttccttgcc gtaggtggcg ctctttacga
     7741 ggtcttgata gtctgacggc atgatgtcgt acagagcgtc tgagatcttg atccgttctt
     7801 tgctcatgaa ggcctcctca ggggaactca atcggaatta gttttctcgt caatattccg
     7861 cggctcagct tccgcgacca gccaacgaca tggtccgcat gccaagtgcg gtcttgattt
     7921 cggacacgat gatttccggc ggcatcgtca tgcctccgca cacgtgcggg ccagcatgga
     7981 ctcgctggtg atttgggatt gatgcgcgaa tttcttttgc cagccagcct gtcacgttga
     8041 attccggcac gaagatgtgc ttcgcgttct tcgtggcttc gcgaatttcc tcttccggcc
     8101 atgggcgcaa cgtcttgacc ttcactaggc cgcaccgaat accttcgtct tccagtaagc
     8161 gaattgcctc acgcccttga gacaccgcag taccagacgc cacgatcatg atgtccgcat
     8221 cggtgttttc ggtgtcgatg agcccgttca accaatggat cgagtgcttc cgggaccgtt
     8281 cgacagccgc ccacacttcc tgttgccagc ttgcatgggt ggcatagctg atgtagttgc
     8341 tcttcatgat aaacggatcg cgcatcattc ggaccggcgg acattccata tccatgcagg
     8401 ggaccggtga gcgataggga tcatagggcg gcaagcacat gtcggccggc gtcaggttca
     8461 caacgtcttt cgtgtgcgtg acgaagaacc catcgcagca gagcgcgagc ggcaaatgca
     8521 cgtccggttc ctcagacacc atgtagccct tgagaatcca atcgaagaag tcctgcgccg
     8581 tctccgcgtg ccacaccagc atgcccgtgt tcaacaggta ggcgatttcc agcgtgtccg
     8641 gctgaatcga cagcggcgag ttgatgccgc ggcaggttac gatcatctgg atcggcagcc
     8701 gtgcgccggc ccacatcggg aagttttcca tggcgcgcat ggtgcccggg ccggccgtcg
     8761 tggtgaacac gcgggcgccg ccgaaggccg cgccggcgca ctgggacatg acggcaaatt
     8821 cgctctctcc gcggaagtag tcaccgatgt agccctcggc gaacaattcg ccgatcagcg
     8881 cggccgcttc gctctgcggc gtgatcgggt aggcgatcat cacgtcgcag ctggcccgcc
     8941 ggagggcttc cttgatcacc tcgctgcccg tgtagaacga cggcgtgcgc ggcgcgtcgt
     9001 tcatcatctg ccaggtatcg
//
LOCUS       DATDNW010000340         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1076821, whole genome shotgun sequence.
ACCESSION   DATDNW010000340 DATDNW010000000
VERSION     DATDNW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076821"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="ssb"
                     /locus_tag="VI359_08185"
     CDS             complement(<1..173)
                     /gene="ssb"
                     /locus_tag="VI359_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY6974268.1"
                     /translation="MAKSVNKVILIGNLGKDPEVKYTPSGMAVANFTLATNERYKDKD
                     GQWQDRTEWHNIVA"
     gene            492..1934
                     /locus_tag="VI359_08190"
     CDS             492..1934
                     /locus_tag="VI359_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6974269.1"
                     /translation="MTLSPANERSAVVARELYASLVEQFKGDLLRPGESGYEEARSVW
                     NGMVSRSPCLIARCADVSDVQMAVKAASAIGILTALRCGGHSLAGFGTCDGLVIDLSR
                     MRAVTLDSDKRRASISGGCLLGSIDSATQRAGLVFPSGVVSHTGASGLILGGGTGWLT
                     RRFGLSCDNVEGFTMVTAEGSLVHCSDTEDSDLFWALRGGGGNFGVVTEFELRLHRLA
                     SVLLGEGLCPEPNIHRLLQFWREFMADAPFDLKWNVDLRLAAQAKNVPIELRGRPVAG
                     SSVLWTGDPQRGRPYLERALSLCHQGSVRTKVMSYLELQTMADLAFPHGRRYYTKSGY
                     FADLDDATIDFLIAALSEIPSPETQIELAYLGAAAAQVSATETAFGDRSAPIIVNLLA
                     NWTEPTLDDANISWVRRLFQRLRPAMKPGVYVNFMSADEQDRVPEAYHERWERIVNVK
                     SHYDPDNFFRLNQNVPPKKSWENGVSAVGK"
     gene            complement(1915..3324)
                     /locus_tag="VI359_08195"
     CDS             complement(1915..3324)
                     /locus_tag="VI359_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY6974270.1"
                     /translation="MSEPTSAGTPASAAAGSTSVPEQEHLHRRLSSRQLTMIAIGGAI
                     GVGLFLGSSLTIELAGPGVIITYVLGALIAIVMAYSLAEMAVVHPVAGSFGIYAEKYL
                     SAWAGFTIRATYGLVEIIAIGAEVTAAAIYFAFWFPHVPGWIWVLFVSAGLITLNALQ
                     VGNFGELEYWFAFIKVFAIVVFIAVGALLITGLGPWRAVGLANLTQHGGFLPHGWRGV
                     WMALTLAITSYMGVEVIAVTAGEAQNPTTSIPRAMRTIVYRLIIFYVLAITVMLAMTP
                     WNQTGSSNINGSPFVRAFQAVGIPFAAGIMNLVVITAALSSANTNLYTSTRMLFSLSR
                     GNYAPAWLGHLSRNGVPHRALAISSAGMIAAILLAIFSPKNAFLFLYGTAVAGMFYVW
                     IVILFTHLRFRRAIAPDVLAGLPLKLPAHPWPTLLALTALIGIALSTFWVKGLEYTIP
                     TFAPLLLLMLVVYAIYRPR"
     gene            complement(3340..>3658)
                     /locus_tag="VI359_08200"
     CDS             complement(3340..>3658)
                     /locus_tag="VI359_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6974271.1"
                     /translation="LAEINNVTVYGIRDRERLDHRVPTVCFNVNGVSAAAVAEHCADH
                     GIGVRDGNMYSPRLLNRMGIPPDTGAVRASLAHYNTVEEVERFIKVMQIIAASASNRR
                     AGS"
BASE COUNT          830 a          988 c         1090 g          750 t
ORIGIN      
        1 gccacgatgt tgtgccactc ggtgcggtcc tgccattggc catctttatc tttgtagcgc
       61 tcgttggtgg ccagcgtgaa attcgccacc gccatgccgc taggtgtgta tttgacctcg
      121 gggtccttgc ccaagttgcc gatgagaatg actttgttga cgctctttgc catgggagac
      181 gctccttctg tcaaaagcca atattttgtg tcaatggaca atatttctgt cggccgacaa
      241 tattgcctgt cagctgacaa aattgcttgt cactggccgg cgtggatatc ctttcccaat
      301 aaccggagta gaccggctgt gtcggaaaca gtgcattcta gcaagtaggc cctccccagc
      361 gtctgcctct ccgagtacac tgtcaagcca caccagcaat tggcggatta cttgcgttaa
      421 acataacaca ccgagcgagc gattttttga accagtaaaa cgaaccgcgc aaactggagc
      481 ggcggcactg agtgacattg tccccggcga atgagagatc agctgttgtt gcgcgagagc
      541 tgtacgcttc actcgtggag caattcaaag gtgatttgct gcgtcccgga gaaagcggct
      601 acgaagaggc acgttcggtt tggaacggca tggtatcacg ttcaccctgc ctgatagcgc
      661 gctgcgcaga cgtaagcgac gtgcaaatgg cggtcaaagc cgcctctgca attggaattc
      721 tcaccgccct ccgctgtggg gggcacagcc tggcaggttt tggcacgtgc gatggtctgg
      781 tcatagatct ctcccgaatg cgcgcagtca cccttgattc ggacaagcgc cgagcgtcga
      841 tcagtggcgg ctgcctcctc ggctcgattg acagcgcgac gcaaagagca ggactggtat
      901 ttccctccgg agtcgtttct cacaccggcg cgtcgggcct gattttgggt ggggggacag
      961 ggtggctcac tcgacgattc ggactgtctt gcgacaatgt ggaggggttc accatggtga
     1021 cggccgaagg ttcgcttgtg cactgcagtg atacggaaga ctccgacttg ttctgggccc
     1081 tgcggggagg tggcggcaac ttcggagtgg tgaccgaatt tgaactgagg ctacatcggc
     1141 tcgcctcggt gctgctcgga gaagggctgt gcccggagcc gaatattcac cgacttctcc
     1201 aattctggag agagttcatg gccgacgccc cgttcgactt gaaatggaat gtcgatctgc
     1261 gtctggccgc gcaagcaaag aacgtgccca ttgagttgcg tggtcggccg gtcgccggca
     1321 gctctgtgct ttggactggt gacccccaaa gagggcgtcc atatcttgag cgcgccttgt
     1381 cgttatgtca tcaaggttct gtgcgtacca aagtgatgtc ctatttggaa ctacaaacca
     1441 tggctgatct cgcctttccc cacggacgcc gctattacac gaaatcaggt tattttgcag
     1501 atctcgatga tgccacgatt gattttttga tcgcagcgct gtctgagatt ccgtcaccgg
     1561 aaactcagat tgaactcgcc tatttgggtg ccgccgcggc acaggtctcg gcgaccgaga
     1621 ccgcgttcgg cgaccgcagc gctcctatca tcgtgaactt gctcgcgaac tggaccgagc
     1681 caactctcga tgacgccaac atttcatggg ttcgccgact attccagaga ctgcgaccag
     1741 ccatgaagcc gggtgtttac gtcaatttca tgagtgcgga cgagcaggat cgtgtgcccg
     1801 aggcttacca cgaaaggtgg gagcgcatag tgaatgttaa gtcacactat gatcccgaca
     1861 actttttccg actgaatcaa aatgttccgc caaagaagtc gtgggaaaac ggggtcagcg
     1921 cggtcggtaa atagcgtata caaccagcat cagcagcaag agtggggcaa aggtcggaat
     1981 ggtgtattcc aagcccttga cccagaatgt acttagtgca atcccaatca gcgcggtcaa
     2041 ggccagtaat gtcggccagg ggtgagccgg cagcttaagg ggcaggcctg ccaagacatc
     2101 tggagctatg gcccggcgaa aacgcagatg agtgaacagg atgacgatcc agacgtaaaa
     2161 catgccagcg acagcggttc catagagaaa caagaatgcg ttcttggggg agaaaatggc
     2221 cagaaggatg gcggcaatca ttcccgcgct ggagatggcg agagctcggt gaggaacacc
     2281 gttccggctc aaatggccaa gccaggcagg tgcatagttg ccccgcgaca gcgagaacaa
     2341 catgcgcgtg gaggtataca ggttggtgtt ggcactggac agagcggcgg taatgaccac
     2401 cagattcatg atgccggcag cgaagggaat tcccactgcc tggaatgccc gcacgaaggg
     2461 actgccgttg atattggaac tgccagtctg gttccaaggc gtcatggcca gcattacggt
     2521 aatggccagc acgtaaaaga tgattaaccg ataaacgatg gtgcgcatgg cgcggggaat
     2581 gctggtggtg gggttctgag cttctccagc ggtgacggcg attacttcca ctcccatata
     2641 gctggttatg gcgagggtaa gcgccatcca tacgccgcgc cagccatggg gcaagaaccc
     2701 gccgtgctgg gtcaaattcg ccaatcctac agcccgccac ggacccaacc cagtgatgag
     2761 caacgctcca acagcgatga aaaccacaat ggcgaagact ttgatgaaag cgaaccagta
     2821 ctccaactcg ccaaaattgc cgacctgaag ggcgttgagc gtgataagtc cggcggatac
     2881 gaacaggacc catatccacc ctgggacgtg gggaaaccag aaggcaaaat aaatggcggc
     2941 ggcagttact tccgcgccga tagcaatgat ctccaccagg ccgtaagtgg cgcgaattgt
     3001 gaagccagcc caggcgctta ggtatttctc ggcatagatt ccgaacgacc cggccacagg
     3061 atgcacgacc gccatttcag ccagcgaata agccatgacg atggcaatca gggcgcccag
     3121 cacataagtg atgatcactc ccggcccggc tagttcgatg gtgaggctgc ttccgagaaa
     3181 gaggcccacg ccaatggcgc cgccaatagc aatcatcgtg agttgccggg aactgagacg
     3241 gcggtgcaga tgctcctgct ccggaacgga agtggagcct gccgcagccg aggccggggt
     3301 accggcggaa gttggctctg acatggtcac tggagatcgc tatgaaccgg cccggcggtt
     3361 ggacgcggag gcggcaatga tttgcatcac cttgatgaac ctttccacct cttccacagt
     3421 gttgtagtgc gcgagggagg cacggacggc accagtgtct ggaggtatcc ccatacggtt
     3481 caagagacgc ggtgagtaca tattgccatc acgaacccca atgccgtggt cggcgcagtg
     3541 ctctgccacc gcggcggcag aaactccgtt gacattgaag cagacggtcg gaacccgatg
     3601 gtcgaggcgc tcgcggtccc gaatgccgta gaccgtgacg ttattgatct cggccaag
//
LOCUS       DATDNW010000341         3955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_519030, whole genome shotgun sequence.
ACCESSION   DATDNW010000341 DATDNW010000000
VERSION     DATDNW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519030"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..694
                     /locus_tag="VI359_08205"
     CDS             110..694
                     /locus_tag="VI359_08205"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY6974272.1"
                     /translation="MKMLTQDDLIPNLDYERQREEFRSRIIELKKRRRISVGPLITLV
                     FENRDTLQFQIQEMIRVEHILDPAKVREELDVYNALLPMPGELSATLLIEIVEQESVK
                     PWLDLFMGLDHGQKVAIRAGKDRVYGEFEGGHSHETKISAVHFVRFKPSAALMATLGR
                     REVPITLSIDHATYHEATVVPWDMRQEWLADLEG"
     gene            699..1451
                     /locus_tag="VI359_08210"
     CDS             699..1451
                     /locus_tag="VI359_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY6974273.1"
                     /translation="MPPVLLTKRIEFAAAHRYIKPEWDEAKNRAVFGRCYNPPAHGHN
                     YMLEVTVAGEVDPHTGMVINLFDLKRVLLTVIEEFDHKNLNLDMPYFKDRIPTSENLA
                     RVLWTKLGTQKDIGTLHAVRLYEDEDLCADVTASGGLEIASITRRFSFTSVHNRNQGH
                     TWDCFVTVLGRIDPNTGMVTDTGVLDRLIQEKIKKVFEGQDLRHVLSTQEVTGESLAK
                     TVWDLLVSSISGGKLEKVRLVQTRDLTFEYSE"
     gene            1780..3174
                     /locus_tag="VI359_08215"
     CDS             1780..3174
                     /locus_tag="VI359_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY6974274.1"
                     /translation="MTSYMRMTRSVPVLALLMVISGSFSEDVQSQIGRFTAKDPGVRT
                     GSAGAGGMISGLTQLEQQVFASAREAFEEVQSVQGSVPNTEAGLGPRFNLDSCAGCHA
                     QPDIGGTSPFLNPQVAVAKKEGANNDIPSFITKDGPVREARFRFNPDGTPDGGVHALF
                     TISGRNDAPGCFLAQPDFRGAVARQNVIFRIPTPTFGAGLIEAIEDDTILANMQANAA
                     TKQSFGIRGRPNTSGRPNTSGNDGTITRFGWKGQNKSLEIFSGEAYNVEQGVTNDLFP
                     QERDEATGCYFNTTPESPTHFDAANPLDIPSDVVKFAVFMRFLAPPTSMPDTPSIVQG
                     RKLFSDIGCVLCHTPTLHTSKKAAVGALRDKDANLYSDLMLHNMGSGLADDVNQGNAR
                     GDEFRTAPLWGLGKRIFFLHDGRTKDLLEAIQAHAGGASGQYPPSEANQVIAQFNRLN
                     EIDKQNVLNFLRGL"
     gene            3475..3663
                     /locus_tag="VI359_08220"
     CDS             3475..3663
                     /locus_tag="VI359_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974275.1"
                     /translation="MNVVACSLTCVSFRLLPACVAVRDVDGKTRLVLSVDQDGFPSLV
                     VRDRDHNTNEFLPIIKGE"
BASE COUNT          905 a         1054 c         1118 g          878 t
ORIGIN      
        1 gtcccctggc aggcctccag cttgaccagg ctggggcgat ggcccatacg ggtggacggg
       61 cgaccaagca cccgattcaa atcgttcgcg atgcctatgg gcttccgtca tgaagatgtt
      121 gacgcaagac gacctgatcc cgaatttgga ctacgagcgg cagagggagg aatttcgctc
      181 ccgcatcatc gagttgaaaa agcggcgccg gatttctgtg gggccgctca ttacgctggt
      241 ctttgaaaac cgcgacacgc tccagttcca gattcaggag atgatccgtg tcgagcacat
      301 cttggatccg gcgaaagtgc gtgaggagct ggacgtctat aatgcactgc tcccgatgcc
      361 gggtgagttg agcgcgaccc tgctgatcga gatcgtcgag caggaaagcg tgaagccgtg
      421 gctggatctc ttcatggggc tcgatcacgg tcagaaggtc gcgatccgtg cgggaaagga
      481 cagagtctat ggtgaattcg aaggtggtca tagccacgag actaaaatca gcgcggtgca
      541 tttcgtgcga ttcaaaccgt ctgcggcgtt gatggctacg ctcggacgcc gggaagtccc
      601 gattacgctc tcgatcgatc atgccaccta tcacgaggcg acagttgttc catgggacat
      661 gcgtcaagag tggctcgccg atttggaggg ataacagcat gccgccggtt ctgttgacca
      721 aacgcatcga gttcgccgcc gcgcatcggt acattaagcc ggaatgggac gaagccaaaa
      781 accgcgccgt gttcggacgc tgttacaacc caccggcgca tgggcacaat tacatgttgg
      841 aagtaactgt ggctggtgaa gtggatccgc acacgggcat ggtcatcaat ctcttcgatt
      901 tgaaacgagt cttgctgacg gtcatcgagg agttcgatca caagaatcta aatctcgaca
      961 tgccatattt taaagatcgg attcccacgt cagagaatct cgcgagagtc ctttggacca
     1021 aactcggaac gcagaaggac atcgggacgc ttcacgccgt tcgcctgtac gaagacgagg
     1081 atctctgcgc ggatgtgact gcgtcgggag gacttgagat cgccagcatc acgaggcgct
     1141 tttctttcac ctcggtgcat aacaggaatc aaggccacac gtgggattgc ttcgtcacag
     1201 tccttggacg aattgatccc aataccggga tggtgaccga caccggggtg ctcgatcgtt
     1261 tgattcagga gaagattaag aaggtattcg aagggcagga tttacgacat gtgctcagta
     1321 ctcaagaagt taccggcgaa tcactcgcga aaaccgtctg ggatctactc gtcagttcca
     1381 tctcgggggg aaagctcgaa aaagttcgtc tcgtccaaac ccgcgatctg acattcgagt
     1441 attcggaata aacggttagc tcacctactc ttcttctgtc tatcagctcc tggtccacat
     1501 gttacaggcg taggcgacgc ttttaaaaga atcggagaag cctacttcag catagagtcc
     1561 cgttctaatg atttgatgtt catgctgtag ggcactgccg acgcttcctc cactcacgcc
     1621 gcggacagat tttgtcctaa tctttccaag ctacaggttc acaaggactt tgaactatcg
     1681 gggcgggtca gcatcgatag ggcacggctt atgctcacct tccgctgtgc gaatgttcat
     1741 tggtgagcgc gtcgataacc ctactacacg gagggctcca tgacctctta tatgcgtatg
     1801 acacgctccg ttcctgtgct ggcgctactg atggttatca gcggttcatt ttccgaagac
     1861 gtccagtcgc aaatcggccg attcacagca aaagacccag gcgttcgcac tggttcggct
     1921 ggtgccggag ggatgatttc gggtctgact caacttgagc aacaagtatt tgcgtcggcc
     1981 cgcgaagcct tcgaagaagt gcagtcggta cagggatcgg ttccgaacac ggaagcaggc
     2041 ctcgggccac gcttcaacct agatagttgc gccggctgtc acgcgcagcc ggatatcggc
     2101 gggaccagtc ctttcctcaa cccacaggtg gcggtggcca aaaaagaggg tgcgaacaac
     2161 gacattccat ccttcatcac gaaggatggc ccggttcgcg aagcacggtt caggttcaat
     2221 ccggacggta cccctgatgg tggagtccac gccctgttca cgatcagcgg tcgtaatgat
     2281 gcgccgggat gttttctggc ccagcctgat tttcgaggag ctgtggcacg ccaaaatgtg
     2341 attttcagga ttcctacccc gaccttcggt gccggattga ttgaagcgat cgaggatgac
     2401 acgattctcg ccaacatgca ggctaacgcc gctacaaagc agtccttcgg tattcggggt
     2461 cgtcccaata ccagcggacg gcccaatacg agcggcaatg acggtacgat cactcgtttt
     2521 ggatggaaag ggcagaacaa gtcgctcgaa atattttcag gtgaggccta taacgttgaa
     2581 cagggcgtca cgaacgatct attcccgcaa gagcgagatg aagcgacagg ctgctatttc
     2641 aacaccacgc cggagagccc gactcacttc gatgctgcga acccactcga catacccagc
     2701 gacgtggtaa agtttgccgt cttcatgcgg tttctcgctc ctccgacgtc gatgccggat
     2761 actccatcca ttgtgcaggg acgcaagctg tttagcgaca tcggctgcgt tctgtgtcac
     2821 acgccaacgc tgcatacgag caagaaggcg gccgttggag cgttgcgcga caaggatgcg
     2881 aacctttact ccgacctgat gcttcataac atggggtcgg gtttggccga tgatgttaac
     2941 cagggaaatg cccgcggcga tgaattccgt acggccccct tatgggggct tgggaagaga
     3001 atcttcttct tgcacgatgg gcgtacgaaa gatctgctcg aagccattca agctcatgcg
     3061 ggaggggcaa gcggtcaata tccgccctca gaagccaatc aagtcattgc gcaattcaat
     3121 cgactgaatg agatcgacaa acagaacgtg ctgaacttcc tgcgtggttt gtgatcacaa
     3181 tgcggagcga ggtggagata atccacctcg ctccgactaa gccaagacct agctaggtag
     3241 taccgaagca tcacgtgtgt aggagatccc aacctcgttt gtgcctatcc ggggacccca
     3301 cccgcatcgg agtgaccggt gagagtgtag gtgactctct agccgaaaca ttctcaattg
     3361 tctcttcgga tcgacgccag ccagagtcaa ggacgactag gaatctggag caagtggtcc
     3421 tttagcctat cgcgctctag acggtgctca tgcacggatg aggatatgac atgagtgaac
     3481 gtcgtagcct gttcattgac ctgtgtctcg tttcggctcc ttccggcgtg cgtagccgtg
     3541 cgtgacgtcg acgggaagac ccgtctggtc ttgagcgtcg accaggacgg atttccatcg
     3601 ttggtggtgc gagaccggga ccataacacc aacgagttcc tacctatcat caaaggggaa
     3661 taaaaaaagc cgatcgctcg gcctactctt acaattctcc tagacctctg tgcagagacg
     3721 ttggaggatc ttgaataact cggggtaagg gtctgcggga ttggctctgg tcagccgatg
     3781 caagctttct gtgcctgatc ccctgcgtgt acgcagggga tcaggccatc cgactgggtc
     3841 ttattgaacc atgggaactg tctggtccga cgagccggtc cagtttgatc gtcctccaag
     3901 tatgctcgac gctagacctt gagcgagatt accctctccg ccaaggatcg tagaa
//
LOCUS       DATDNW010000342         9028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1148983, whole genome shotgun sequence.
ACCESSION   DATDNW010000342 DATDNW010000000
VERSION     DATDNW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148983"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1138
                     /locus_tag="VI359_08225"
     CDS             173..1138
                     /locus_tag="VI359_08225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974276.1"
                     /translation="MEERKTGGDHYERGRVLKHNQMFKEALKEFQEAATDPQYVGNAY
                     VQIALCLRTIDHGDAAVTAFRRALTLGTFSSKEKAHIFYFLGQTLESLGRYPEALEAY
                     GWSRKEDPCLEDVAHRIKHLVSGGRGPLPQPRLGSQPWVGGTLKLGRRLTLQVLSALG
                     QTWESFSGSAGTLKTEPRAKGQSANFRQVGHQNGHRTLTPNHASAQASWDRKKDKRQH
                     ARVAVHLRSHFSSKGQMLAGDGELRDLSLGGCRVTSSVAVPVGVMLECCIYPQGAGNP
                     FTIEGALVRWSRPQEFGLAFTTVGPRVQRQIAQLCGTRTPLGVAI"
     gene            complement(1292..1368)
                     /locus_tag="VI359_08230"
     tRNA            complement(1292..1368)
                     /locus_tag="VI359_08230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1332..1334),aa:Arg,seq:tct)
     gene            complement(1603..1679)
                     /locus_tag="VI359_08235"
     tRNA            complement(1603..1679)
                     /locus_tag="VI359_08235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1643..1645),aa:Pro,seq:tgg)
     gene            complement(1756..2364)
                     /locus_tag="VI359_08240"
     CDS             complement(1756..2364)
                     /locus_tag="VI359_08240"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEY6974277.1"
                     /translation="MIHELVLATRNRQKGEELAALLGDLGIRIRTLDEFPEAPEVIED
                     GETCEANAIKKARAIADATGLPAVADDTGLEVDALEGRPGIFAARYAGEGATYEDNYR
                     KLLHELAGVPRERRAARFVTVAAMAFPTDGVQVARGVLEGLIAEVPSGTQGFGYDPVF
                     FVPELGKTLAQLSADQKNRISHRAKAFAKVREILCSQMVNVE"
     gene            complement(2376..3119)
                     /gene="rph"
                     /locus_tag="VI359_08245"
     CDS             complement(2376..3119)
                     /gene="rph"
                     /locus_tag="VI359_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY6974278.1"
                     /translation="MATAGGLLRVDGRRRDQLRPVKVTRNFIKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGTGWVTAEYSMLPRSTHERSPREAVKGRQGGRTLEIQRLVG
                     RALRAVTDMSQMGERSIWIDCDVIQADGGTRTASITGSFVALADAFAVLKKRDLVKNR
                     PLTDYLAAISVGKVGGEVLVDLAYTEDVAAEVDMNLVMTGRGRYVEIQGTAERTPFGK
                     QELDEFMSLGWTAIQTLTALQQDLVGSLD"
     gene            3515..4657
                     /locus_tag="VI359_08250"
     CDS             3515..4657
                     /locus_tag="VI359_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY6974279.1"
                     /translation="MTLLLDRSSQQAALDLKRILVIDDEEPIRRLLGYLLQTHGYETV
                     LASDAREARQRLDEQPFALILCDVNMPGESGMDLVRTILREHPHTAIIMVTGLDSSVL
                     ANAALEMGAFGYIIKPFESNEVLIDVANALRRRRLEMENRLQRENLEDVVRTRTMALQ
                     QALEWLERSEKELRLSREETIQRLAIAAEYRDSATAQHIQRMSHYCEMLARRYGLSNE
                     RCDLIRTASPMHDIGKIGTPDHVLLKPGKFTQEEFNVIAQHAEIGYRILGGSDAEILK
                     VAATIAWTHHERWDGSGYPRKLKGEAIPVEGRIAAIADAFDALTTQRVYKPAFELSHA
                     VDLMSKHRGEHFDPELLDVFFASIDEIKRIHELYADRSPRGMGLEQ"
     gene            4794..5306
                     /locus_tag="VI359_08255"
     CDS             4794..5306
                     /locus_tag="VI359_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974280.1"
                     /translation="MAKKTSAARKPATTRNWIAYLCESLVTSGEMPTWEAATYLTENL
                     FAEKPNPRLLGTTNAFEVTSQFLQRLYHPLVEAVSRDLSLPQAAKLHVFTDISLTGKS
                     AVLVVYFPGNNQSHQLLMLDAAKAWELVFTDSEDFNRWAEERYRSVKSALLVAIGKPM
                     NSTLLVGLSA"
     gene            complement(5314..9021)
                     /gene="metH"
                     /locus_tag="VI359_08260"
     CDS             complement(5314..9021)
                     /gene="metH"
                     /locus_tag="VI359_08260"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY6974281.1"
                     /translation="MVEIHQNASLLDALLKTRILVLDGAMGTMIQQRRLDEAAFRGER
                     FKDWKRDLKGHNDLLNLTQPEIVQDIHRQYLEAGADIIETNTFNSQSISLADYHMESL
                     AYELAQAGAECAKRAVAQVCTAQAGRRCFVAGAIGPTTKTSSISTDVNNPAARGTTYD
                     ELVSAYFDQVRGLVDGGVDILLVETIFDTLNAKAAFFAIEQFFDERRLRLPVMASVTF
                     IQAGSNRGVTGQTVEAFWNSVSHVPLLSVGINCALGPKEMRPLIEELSQIAPIYVSSH
                     PNAGLPNPLLPTGFPETPDTLAPQLKEWARSGWLNIVGGCCGTTPAHIKLIAEAVRGL
                     PPRVPPLIEPYTRLSGLDALTIRPESNFVNIGERTNVTGSPAFARLILGGDYDSALSV
                     ARQQVEGGAQIIDINMDEGLLDSKAAMEKFLRLVGSEPDISRVPVMVDSSKWEILETG
                     LKNIQGKAVVNSISLKEGEAKFIEQATLIRRYGAAMVVMAFDERGQADTLERKTEICA
                     RSYKILTERVGVPPQDIIFDPNVLTVATGIEEHNNYAVNFIETTRWIKQHLPLAKVSG
                     GISNISFSFRGNNVVREAMHAAFLYHAIKAGLDMGIVNAGQLAVYEEISQDLLTLVED
                     VLLNRRPDATERLVAFAETVKQKGKATVKDDEWRAGAVEDRLAHALVRGITDYIDQDV
                     EEARRKYAKPLEIIEGPLMTGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFME
                     EEKERLGQARANGRVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPCEKILST
                     ARDQKADIVGLSGLITPSLDEMVHVAKEMTREGFTVPLLIGGATTSKAHTAVKIAPSY
                     EPSVVHVLDASRAVGVVGSLMNPGQKSEFVNQVRNDYDRVRQAHQDRTAKPVLPIARA
                     RANRFTSDWTAIEIPVPSFLGLKVISDQPLGELVPFIDWSPFFHTWELKGRYPTIFND
                     TTIGPKAKELHDDALHLLERIIKRKLLTARAAYGFFAAASIGDDIQLYSDESRKKPLT
                     VIHTLRQQSEKPQGQPNLALADFVAPADSGRKDHIGVFAVTAGIGLDDLCRQFDKDHD
                     DYNSIMAKALADRLAEAFAEWLHKRVREDWGYGKGEKCSHEDLIREKYRGIRPAPGYP
                     ACPDHTEKRLLFDLLQAEQNTGITLTESFAMLPAAAVSGFYFAHPEAKYFAVGKIGKD
                     QIEDYACRKGMDLRTVERWLGPNLNYEPSES"
BASE COUNT         1933 a         2730 c         2352 g         2013 t
ORIGIN      
        1 taccgtttcg gccagcctgt tggactcttc caccccctga gcgcaatttg accaggcaag
       61 tgattgattt gtggggccag cgtggctctc cagcagggcg ttattcttgc tcctttcccc
      121 ctcggccaac atttgtcacc tggaacggcg tcacaaaggg gaggatgcaa gcatggaaga
      181 acggaagacc ggaggcgacc actatgagcg cgggcgtgta ctcaagcaca accaaatgtt
      241 caaggaggcg ctcaaggagt tccaagaagc ggccacggac ccgcagtatg tcgggaacgc
      301 gtatgtgcag atagcgctgt gcttgcgaac gattgatcac ggcgacgccg ccgtcacagc
      361 cttccgccga gccctgacgt tgggcacgtt ctcctccaaa gaaaaagccc acattttcta
      421 ttttctcgga cagacgttgg agtcactcgg ccggtatccg gaagcgctgg aggcctatgg
      481 ctggagcaga aaagaggacc cctgccttga ggacgtggcg caccggatta aacatctggt
      541 ctcaggtgga cgcggcccgc tcccccagcc tcgactgggc tctcagccct gggtcggagg
      601 cacgctcaaa ctagggcggc gactcacgct gcaagttctt tctgcgctgg gacagacctg
      661 ggaatcgttc agtgggtctg cgggcacgct gaagaccgag ccaagggcca aaggccagtc
      721 tgccaatttc cgacaggtgg gccaccagaa tggacaccgg acactcacgc cgaatcacgc
      781 cagtgcgcag gcgtcatggg accgcaagaa agacaaacgg cagcatgccc gcgtggcagt
      841 ccacttgcgc agccactttt cctcaaaggg tcagatgctg gcgggtgacg gagagctgcg
      901 agacctctcc ctggggggct gtcgggtcac cagctccgtg gccgtccccg tgggagtcat
      961 gttggagtgt tgtatctatc cgcagggtgc cggcaacccg ttcacgattg aaggcgcgct
     1021 ggtgcgctgg agccgtccgc aagaattcgg cctggccttc accacagtcg gtcccagggt
     1081 gcagcggcag attgcgcagc tgtgcggaac acggaccccg cttggggtcg caatttagga
     1141 ccgggctcag cgttgggccg agttatgcct cgggttagat tgattggatt tctacggaag
     1201 gactatcaga gagcaccctt cagtttgcac tacccgcgca ctacactccc tttcgattct
     1261 cgtatctcca aaactaagct gtcgaaattt ctggtgcgcc cggctggaat cgaaccagcg
     1321 accctcagct tagaaggctg atgctctatc caactgagct acgggcgcgt gaacacgtga
     1381 ggtctttatc cgcggacagc tgtgacaata atacgatgaa gagtccgcgg cacgagctag
     1441 cttgatttgg tcggggtgag aggatttggt ctccaggtcc aggctacgcc tgcccttccg
     1501 accggccggc tcgctgccgg gcagttatgg cccttccctc cctattcggt cggcgctcgc
     1561 cggcttcaaa ccctctcgac agcccatctt atttttgcac tttggtcggg gcgagaggat
     1621 ttgaacctcc gacatcctgc tcccaaagca ggcgcgctac caggctgcgc tacgccccga
     1681 ctgttttcgt agcaatattg aacggtcaat gtctatgtag accctgctcc aaatcgtccg
     1741 acaattcaac attcatcatt caacattcac catttggcta cagaggatct cgcgcacttt
     1801 tgcaaacgct ttcgcccgat gactaatgcg attcttctgg tctgcggaca gctgagcgag
     1861 tgtcttcccc agttccggaa caaaaaatac cggatcatat ccgaatccct gagtgccgct
     1921 tggcacttcc gcgatcagcc cctccaacac gccccgtgca acctggactc catccgtcgg
     1981 gaaggccatt gctgcaaccg tgacgaaccg agctgcccgc cgttcacgcg gcacgccggc
     2041 aagctcatga agcaacttac gataattgtc ctcataggtt gctccttcac ccgcgtaacg
     2101 ggcagcgaaa attcctggac gtccctcgag cgcgtcgact tccaaccccg tatcgtcggc
     2161 aacagccgga agtcccgtcg catcggcgat cgcccgtgct ttctttatcg cattggcctc
     2221 acaggtctct ccgtcttcaa tgacttccgg ggcttcagga aattcatcga gggttctgat
     2281 ccgtatcccc aaatcaccca aaagagcagc caattcttct cccttctgtc gatttcgcgt
     2341 ggcgagcaca agctcgtgga tcatctttgc ctcaattaat ctaatgatcc aactaaatcc
     2401 tgttgcagtg cagtcagcgt ttggatcgcg gtccatccaa gggacatgaa ctcatccaat
     2461 tcttgcttcc cgaacggagt ccgctccgcg gtgccctgaa tctcaacgta gcgtccacgc
     2521 ccggtcatga caagattcat gtcgacttcc gccgccacat cctcagtgta ggccagatcg
     2581 acaagaacct cccctcccac ctttcccacg ctgattgccg ccagataatc ggtcaacggc
     2641 cgatttttga cgaggtcgcg ttttttcagc acggcaaagg catcggcaag cgcgacgaac
     2701 gaccctgtga tggaggcggt tctggtcccc ccatccgctt gaatgacgtc gcagtcgatc
     2761 caaatcgacc gttcgcccat ctgactcatg tccgtgacag cccgaagcgc gcgcccgacc
     2821 aatcgctgga tctcaagggt ccgcccaccc tgcctcccct tgacggcttc acgcggcgaa
     2881 cgctcgtggg ttgagcgggg gagcatggaa tactccgccg tgacccatcc agttccctta
     2941 cctttcagga acggaggcac tttttcttcg atcgacgccg tgcaaatgac tttggtatcg
     3001 cccatttcaa tcaggaccga tccttcagca tgtttgataa aatttctcgt caccttgacc
     3061 gggcgaagct gatcccgtcg tcgcccgtcg acccgcagta atcccccggc agttgccatt
     3121 atgttctccc gtttacgatg ccgcgcatta tagtaaaggc cttctgaaag cgcaacgaaa
     3181 ccgcggccac ggcctccaag aggtcacacg caccccgctg atagccacat cacgttcgta
     3241 caattctgcg acgacgttga cacgaattct gtacatagtt ctcacttttc agcggacaca
     3301 aatattgact atttcgttca tgtcatcaca acacatcgaa accccacctc taacgcggag
     3361 tttcttctcc aaaacaagta cgagaccgca tacgcgcgcc cggaagatcc gcagaaactc
     3421 ataatttatt ggcacaaaag gtgcacctgg atttacagtg ccttagacac cccgcgccaa
     3481 cgaaactgag cctgagctgt cgatcgcttt gcccatgaca ctgctgttgg atcgttcctc
     3541 ccagcaagcc gccctcgacc tcaaacggat tctcgtcatc gacgacgagg aacccattcg
     3601 tcgtttgtta ggttatttgc tccagacgca cgggtatgaa acagtcctgg cctccgatgc
     3661 tcgcgaagcc cgccaacgat tggacgaaca acccttcgca cttattttat gcgacgtgaa
     3721 catgcctggt gaatccggaa tggatctcgt tcgtacgatt ctcagagaac atccgcacac
     3781 ggccatcatt atggtcacgg gcctagatag ttcggtgttg gcaaacgcgg ctctggagat
     3841 gggtgcattc ggctacatta tcaagccgtt tgaatccaac gaagtcctca tcgacgtagc
     3901 caacgccctg cgccgccgac ggcttgagat ggaaaaccgc cttcaacgcg aaaacctgga
     3961 agacgtcgtc cgcaccagga cgatggcgtt acaacaggcc ctggagtggt tggaacgaag
     4021 cgagaaggag cttcgccttt cccgtgaaga aactattcag cgattggcga ttgcagccga
     4081 atatcgcgac agcgccacgg cacaacatat tcaacggatg agccactatt gcgaaatgct
     4141 cgccaggcgc tacgggctct caaacgagcg ctgcgatctg atccggacgg ccagtccgat
     4201 gcacgacatc gggaagatcg gcactccaga ccatgtcctc ctcaagcctg gcaaattcac
     4261 tcaggaagaa tttaatgtga tcgcacagca tgctgaaatc ggttatcgca tcctcggtgg
     4321 ctcagatgcc gagattctga aggtcgcggc cacgatcgcc tggacgcacc atgaacgctg
     4381 ggacggcagc ggttatccgc gcaagctcaa aggcgaagcc attcccgtcg aaggacggat
     4441 cgccgcgatc gccgatgcct ttgatgccct caccacgcaa cgcgtctata aacccgcctt
     4501 cgagctaagc catgccgtcg atcttatgag caagcaccgc ggtgaacact tcgatcccga
     4561 actcctcgat gtcttttttg cctccatcga cgaaatcaag cggattcatg agctgtatgc
     4621 tgaccgatca ccccgcggca tgggtctcga acagtagtcg acattcccgc atttcgttga
     4681 ccccttctca tctcgcgcta tacttcgtgt aaatcggact ggccgcgtcc tcctacttca
     4741 tcaccaggcc cggacgactc gtgtttgcca cgacgacgaa aggattgcct cccatggcaa
     4801 agaaaacctc agccgcgcgg aaaccagcaa caaccagaaa ctggatcgcc tatctctgcg
     4861 aatcattggt gacctccggt gagatgccga catgggaagc cgcaacctat ttgaccgaaa
     4921 atctgtttgc tgaaaagccg aatccccgtc ttcttggcac tacgaatgcg ttcgaggtga
     4981 catctcaatt tctccaacgg ctgtatcacc ctctcgtgga agctgtatca cgcgatctct
     5041 cattgcccca ggccgcgaag ctgcatgtct tcacggatat cagtttgacg gggaaatccg
     5101 cagtcctcgt cgtctatttc ccgggaaata accaatcgca ccagctgctg atgttggatg
     5161 cggccaaggc ttgggaattg gtcttcaccg actccgagga cttcaaccgg tgggccgaag
     5221 agcgatatcg gtcggtcaag tccgccctgc tcgtggctat cggcaaaccc atgaactcga
     5281 cgctactggt gggcctttcg gcgtgagatt cttctacgat tcggacggtt cgtagttcag
     5341 attgggtccc agccatcgct cgacggtacg cagatccatc ccctttcggc aggcgtaatc
     5401 ttcgatttga tccttcccga tctttccgac tgcaaagtat ttcgcctccg gatgagcgaa
     5461 ataaaaaccg ctgactgccg cggccggcaa catggcgaag ctttccgtca acgtgatacc
     5521 ggtgttctgc tctgcttgta gaaggtcaaa caggagtcgc ttctccgtgt ggtccggaca
     5581 ggccggataa ccaggcgcag ggcgaatgcc gcgatatttc tctctgatca ggtcttcatg
     5641 tgaacacttt tctccttttc cgtatcccca gtcttcgcgc actcgcttgt gcagccattc
     5701 cgcgaaggct tcggccaatc gatcagccaa cgcttttgcc ataatcgagt tgtagtcgtc
     5761 gtgatctttg tcgaactggc ggcacaggtc atcgagacct atcccggcgg tgactgcaaa
     5821 gaccccgatg tgatcttttc ggccagaatc tgccggcgcg acaaaatccg caagcgccag
     5881 gttcggttgc ccctgcggtt tttcggactg ttgccgcaac gtgtgaatga cggtcagcgg
     5941 tttctttctg ctttcgtcgg aatagagctg gatatcgtcg ccaatgctcg cggccgcaaa
     6001 gaagccgtac gctgcccttg cagtgagaag cttccgcttg atgatccgtt caagcaaatg
     6061 aagagcgtca tcgtgcagct ccttcgcttt gggaccgatc gttgtatcgt taaaaatcgt
     6121 cggatagcgc cccttcaatt cccaggtatg aaagaacggc gaccaatcaa taaaagggac
     6181 cagttcgccg agcggctgat cgctgatgac ctttagtccc agaaacgacg ggactgggat
     6241 ctcgattgcg gtccagtcgg atgtaaaacg gttcgcgcga gcgcgggcaa ttggcaagac
     6301 aggcttcgcc gttcgatcct gatgcgcctg acgaacacga tcgtagtcat ttctgacttg
     6361 gttcacaaat tcggatttct gccccggatt catgaggctc cccaccacac cgaccgcccg
     6421 tgacgcatcc aggacatgga ccacggacgg ctcataggac ggtgcaattt tgaccgcggt
     6481 atgcgccttg ctcgtggtgg cgccgccgat caacagtgga accgtgaagc cctcacgggt
     6541 catctctttg gctacatgca ccatctcgtc gagtgatggc gtgatgagac cgctcaagcc
     6601 gacgatatcc gctttttggt cgcgagccgt actgaggatc ttttcgcacg gcaccatcac
     6661 accgagatcg atcacttcat agttgttgca ccccaacacc accccgacga tgttttttcc
     6721 aatgtcatgg acatcgcctt tcaccgtcgc cagcaacact ctgccgtttg ccctggcctg
     6781 acccaacctc tccttctctt cctccatgaa tggcaggaga taggccacgg ctttcttcat
     6841 gacgcgggcg cttttcacca cctgcggcag gaacattttt ccggatccga acaagtcacc
     6901 gacaatattc atccccgtca tcagcggtcc ctcgatgatc tcaagaggct tcgcatattt
     6961 ccgtcgagcc tcctcaacat cctgatcgat ataatccgtg attcctctga ccagcgcgtg
     7021 ggccaatcga tcctcgaccg ctcccgctcg ccattcatcg tccttgaccg tcgctttccc
     7081 cttctgtttg accgtttccg caaacgcaac gagccgttcc gtcgcatccg gacgacggtt
     7141 gagcaggacg tcttcgacga gtgtgagcag atcttgggaa atctcctcat acaccgccaa
     7201 ctggcccgcg ttgacaatcc ccatatccag accggccttg atggcatgat agagaaaggc
     7261 cgcgtgcatg gcttcacgaa ctacattgtt gccgcgaaac gaaaaggaga tattgctaat
     7321 cccgccgctc accttggcca agggaaggtg ctgcttgatc catcgggtgg tctcgatgaa
     7381 attcaccgca taattattgt gctcctcaat ccccgtcgca acggtgagaa cgttcggatc
     7441 gaagataatg tcttgcggtg gaacgcccac acgttcggtg agaatcttgt aggaacgcgc
     7501 acatatctcg gtcttgcgtt cgagcgtatc cgcctggcca cgctcatcga atgccatgac
     7561 gaccatggcc gcgccatacc gccggatcag ggtggcctgc tcgataaact tcgcttcgcc
     7621 ttctttcaag ctgatgctgt tcacgacagc cttgccctga atattcttca ggccggtctc
     7681 aagaatttcc cacttcgaac tatcgaccat cacaggcaca cgagagatat caggctccga
     7741 ccccactagc cgaaggaatt tctccatggc cgctttcgaa tccaggaggc cctcgtccat
     7801 gttgatatcg ataatctgag cgccgccctc cacttgttgt cgagccaccg agagggcact
     7861 gtcgtagtcg ccgcctaaaa tcagtctggc aaaggcgggc gatcccgtga cgttggttcg
     7921 ttcgccgata ttgacgaagt tcgattccgg ccggatcgtg agagcatcga gtccgctcaa
     7981 gcgtgtgtac ggttctatca aaggtggaac tctgggaggc agcccccgga cggcttctgc
     8041 gatcaacttg atgtgggctg gtgtcgttcc gcagcagccc ccgacgatat tcagccaacc
     8101 gcttctcgcc cattccttga gctgtggggc aagggtgtcc ggcgtttccg gaaatcctgt
     8161 cggcaacaac ggattcggga gcccggcgtt cggatggctg cttacataga taggggcaat
     8221 ctgggacagc tcctcaatga gcggacgcat ttctttcggc ccgagcgcac aattgatccc
     8281 cacgctgagc aacggaacat gtgagacaga gttccaaaag gcctcgactg tttgccctgt
     8341 cacgccacga ttgctgccgg cctgaataaa cgtcacggag gccatcacag gcaaccgcag
     8401 cctccgctcg tcaaagaatt gctcgatggc gaagaacgcg gctttggcgt tgagcgtatc
     8461 gaaaatggtt tctacgagca ggatatcaac gccgccatcg accaggccgc ggacttggtc
     8521 aaagtaggcg ctcaccagct catcgtacgt ggtaccacgg gccgcggggt tattgacgtc
     8581 agtggagatc gacgacgttt tggtggtggg cccgatggct ccggcgacaa aacatcgtcg
     8641 ccccgcctgt gcagtgcaga cctgagctac ggcccgcttc gcacactcgg ctccggcctg
     8701 tgccaattca taggccagtg attccatgtg gtagtcggcc aacgaaatcg actgcgaatt
     8761 aaacgtgttg gtttcgatga tgtcggcgcc ggcctcaaga tactgtcgat ggatatcttg
     8821 gacgatctca ggctgggtga ggtttaagag atcattgtgc cccttgagat ccctcttcca
     8881 gtctttgaaa cgctcacccc gaaatgcagc ttcgtccagc ctccgctgct gaatcatggt
     8941 tcccattgca ccatcgagga ccaaaatgcg agttttgagg agagcgtcga gcaacgatgc
     9001 gttttgatgt atttccacca tgatttct
//
LOCUS       DATDNW010000343         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_258934, whole genome shotgun sequence.
ACCESSION   DATDNW010000343 DATDNW010000000
VERSION     DATDNW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258934"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VI359_08265"
     CDS             complement(<1..796)
                     /locus_tag="VI359_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974282.1"
                     /translation="MKKGVLSFMFGMAAVALVAAPLAANAGGTIAGKVTYAGKSEQKE
                     FLFSKFPNPKFCPKNPNKSLMDGDKRFLKTIEVGKDGGLKGAVVAVTDVEDKAFVDGY
                     AGTEVTAAFCEFLPFSGVVVNTRPFKVENTDSDPDDPKSTMGVLHNPHSFTVKGSTSA
                     TGFNIGLAKKGDKLDKPVTFRGGAEKDGYYRLQCDQHEFMQSFFLPVSNAHYAVVKDD
                     GSFELKDVPAGKHKVVAWHPFAAKGKKVEFEVEVPEGGTANLKAEIK"
     gene            complement(1012..2409)
                     /locus_tag="VI359_08270"
     CDS             complement(1012..2409)
                     /locus_tag="VI359_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6974283.1"
                     /translation="MKGQLMRFDLPVQSRLFLVVILVWLSGWGDGAGSLSAASSSVPK
                     EIQSAYDGKQYQQVVDELAKLNPEQRAVPDVRRVAIRSLLKLGNPKDALTEYDQLEAT
                     LKGEDPVLLHEVALGFIVVLLKDMREQMRGAAYTALKEIDPAEALPYFEDGLSDGSGP
                     VRVLAVEGLGRSEVGRKSTKLRTALEDQAGIVKARAVKALGRTGDRSVIPLIEPATKD
                     EMAVVRVTAYGALVRLGRKEMWESLRQAAEAANPEDRAEALRVMVDLKDPRAIPIMLQ
                     LLNYTQPSVRGTAARGLGQLGHHEARRQIEQLLKDPVPAVRESAAASLADLGGKESVP
                     SLTKALSDGNMTVRAATIAALLQLGEPFHTVAPTALALVQQNDTAARASAAHALGKAI
                     KTNAKDAVSLLVSLTTDPLPGPKIVALRSLGHVGDRDVLPVLKEALHDTNEAVRATAG
                     GGVIHLLQRKKHPDF"
     gene            complement(2413..2916)
                     /locus_tag="VI359_08275"
     CDS             complement(2413..2916)
                     /locus_tag="VI359_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974284.1"
                     /translation="MIKKAEGRRHQAQVFAKGDRLRLEYRYALKTERGYAAIEIIRLD
                     KLETWYVLAQRKELLVTPLDPKEVLPIRPMLPGEKQRVLVGAARASGREAQLYEVQTD
                     YHGRAERFFEWVDVETGVVLKLVSQDRDWSVEYERFRVSPQPESYFDEPPGYVKRVSP
                     TGPRIQR"
BASE COUNT          701 a          991 c          797 g          697 t
ORIGIN      
        1 acttgatctc ggccttgagg ttggcggttc cgccttccgg cacttcaacc tcaaactcga
       61 cctttttgcc ctttgcggca aacggatgcc aggcgaccac tttgtgctta ccagccggaa
      121 catccttcag ctcgaaagag ccgtcatcct tgaccaccgc gtagtgggca ttcgagactg
      181 ggaggaagaa ggactgcatg aactcgtgct gatcgcactg taaacgatag tacccgtcct
      241 tctctgcgcc gccacggaag gtcaccggct tgtcgagctt gtcacccttc ttggccagac
      301 cgatgttaaa accggtcgcc gaggtggagc cctttactgt gaagctgtgg ggattgtgga
      361 gtacccccat cgttgactta ggatcatccg gatcggaatc ggtattctca accttgaatg
      421 gtctcgtgtt taccaccacg ccgctgaacg ggaggaactc gcaaaacgct gccgtgactt
      481 cagtgccggc atacccgtcc acgaacgcct tatcttcaac atccgtgacg gcaacgaccg
      541 cgcccttgag accaccatcc ttgccgactt cgatcgtctt caagaaccgc ttgtccccat
      601 ccatcaggct cttgttcgga ttcttgggac agaacttggg gttggggaat ttcgaaaaga
      661 ggaactcctt ctgctcagac ttccccgcat atgtgacttt gccggcaatg gttccgccgg
      721 cattcgcagc aagaggtgcc gcaaccagcg ccaccgccgc cataccaaac atgaatgata
      781 gaacaccctt cttcataaga ccgcctcctt gtgatgaaca ataaccggct gattcgtatg
      841 ctatcgctcc ccggccactc tcgtcagcca gcatggaaac gagagggagc ataagtccgt
      901 cctcttcact cgatcggcat tcccccaaag cggcgaggct ggattttgaa cgcatgtgta
      961 tacagcaata taccccctct cgtcaatctc aaacaagagg gcccacaagt cctagaaatc
     1021 tggatgtttc tttctctgaa gaagatggat cacgcctcct ccggccgtcg cacgaaccgc
     1081 ctcattggta tcatggagtg cttccttcag aactggaagg acatcacgat ctccaacatg
     1141 accgagagac cgcagcgcca cgatctttgg accaggaagc gggtcggtcg tcaggctcac
     1201 gagtaaagac acagcatcct tcgcattagt ctttatcgct ttcccgagcg catgggccgc
     1261 cgaggcgcgg gcggccgtgt cgttttgttg caccaatgcg agtgcagttg gcgcaactgt
     1321 gtgaaacggc tcgccaagct gaagcaacgc tgcgatcgtt gcggcacgta ccgtcatgtt
     1381 tccatcgctc agagccttcg ttaacgacgg aaccgactcc tttcctccaa ggtccgccag
     1441 gctggctgcc gccgattcac ggacagcagg aactggatcc ttcaatagct gttcaatctg
     1501 tctccttgcc tcatgatgtc caagttgccc caaccctctg gctgcagttc cgcgaacgga
     1561 gggctgcgta tagttcagca attgaagcat gatcggaatc gcgcgtggat ctttgagatc
     1621 aaccatgact ctgagggcct cggcacgatc ctccggattg gcagcctctg cagcctgccg
     1681 cagagattcc cacatctcct tccgccccaa ccgcactaag gcaccatagg ccgttacccg
     1741 caccacggcc atttcatctt ttgtcgcagg ctcaataagc gggatcactg accgatcacc
     1801 ggttcggcca agtgctttca ctgcgcgagc cttcacaatc cccgcttggt cttcgagcgc
     1861 agtccgcagc ttcgtggatt tccggcctac ctcggaccga cctagtccct cgaccgccag
     1921 aacccgcacc ggacctgatc cgtcgctcag gccgtcttcg aaataaggca gggcctccgc
     1981 gggatcgatt tccttgagtg cggtgtatgc agccccgcgc atctgctccc gcatatcctt
     2041 caacagcaca acgatgaatc ctagggctac ctcatgcaga agaacgggat cctccccctt
     2101 gagcgtcgcc tcgagctggt cgtactcagt caaggcatct ttgggattgc cgagttttaa
     2161 caacgaacgg atcgcgacgc gacgaacgtc agggacggcg cgttgctcag gatttaactt
     2221 tgccaactca tcgacgacct gctggtactg tttaccgtca taggcagatt ggatctcttt
     2281 gggaaccgag gaagaagctg cggacagaga gccggcacca tcaccccatc cagatagcca
     2341 gacaagaatg accacaagaa acaggcgtga ctgaactggg agatcgaacc tcatcaattg
     2401 acctttcacg gattatcttt ggatgcgagg ccccgtggga ctcacgcgtt tgacgtagcc
     2461 tggcggttca tcgaaataag actcaggctg aggcgagaca cgaaatcgct cgtattcaac
     2521 cgaccaatct ctatcctgac tgaccaactt gagaaccacc cccgtctcaa catccaccca
     2581 ttcaaaaaac cgctcggccc gaccgtggta gtcggtctgc acctcataga gctgcgcctc
     2641 ccgcccactc gctcgcgcag ctcccactaa gactcgttgc ttctctcccg gcaacatcgg
     2701 tcggatcggc aggacttctt tcggatccaa gggggtcacc aataattctt tccgttgcgc
     2761 cagcacgtac cacgtttcca acttatcaag cctgatgatc tcgatggcgg cataccctcg
     2821 ttctgtcttg agggcatacc ggtattccag gcgcaggcgg tctcctttgg cgaacacctg
     2881 ggcctgatgt cgacggcctt cagccttctt gatcaacgaa cctgaaaaat caggagggag
     2941 gttggcatct ccagcccagc cggcactacc tacacccgca catgtaacac agcatgaaat
     3001 cagaacagcg cgcgtccacc accagacatt ctcatggtga gacgcctgca tgagcgctta
     3061 atgttgatgt tcgacaagcg gctggatatc gaccgaatac cccagcgtgt ctggcccaaa
     3121 tcgggggtta tccatcacct tgtaggcgga gcgattgccc ttcggagcca caagtgacaa
     3181 attctc
//
LOCUS       DATDNW010000344         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_259245, whole genome shotgun sequence.
ACCESSION   DATDNW010000344 DATDNW010000000
VERSION     DATDNW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259245"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VI359_08280"
     CDS             <1..403
                     /locus_tag="VI359_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974285.1"
                     /translation="AIQGVMAKRLCKRICSNCKQTYHPSSQEYDKLVRAYGTNAWEAL
                     GISYHEEFQLYRGHGCETCNQTGLKGRVALHELLCGSDELRSLIQNRAKTAEMLSVAI
                     KEGMTTLVQDGIGKVLAGLTTYDQVRAVAMK"
     gene            complement(434..754)
                     /locus_tag="VI359_08285"
     CDS             complement(434..754)
                     /locus_tag="VI359_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974286.1"
                     /translation="MDSRKNIRYAVQLPVSFNGDQLSGEGEGTILNLSADGCAITSTT
                     VAGPASYMQLTMQLRERDEPVRVDLAAVRWSTPARFGVEFIKVRPEEGDRLKRFVKAL
                     ETGV"
     gene            complement(799..1290)
                     /locus_tag="VI359_08290"
     CDS             complement(799..1290)
                     /locus_tag="VI359_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974287.1"
                     /translation="MSGLQLPLFVRRIGLLALSCAVLGVSACTHPAVQRVGSTELVPS
                     PDRALPSDRRNLAGEWEYEDGAVFVLTLDEQGNGPYAWKDGRIETQMLTDRTWKGMWF
                     QKENDREGGFSVEFSSDFSEGDGTWWYTRIGDDHIPSQKGGTFHLTRKSLSAKVSETP
                     PAP"
     gene            complement(1394..>2358)
                     /locus_tag="VI359_08295"
     CDS             complement(1394..>2358)
                     /locus_tag="VI359_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6974288.1"
                     /translation="LPELRQALASKLARYNGIKADPETEITISSGATGSFHCACLALL
                     NPGDEVILFEPYYQYHISALLAVEAVPIIVKMTAPHWTFSTGEIERAMTTRTKAIIVN
                     SPGNPSGKVFSRTELEGIADVTRRHDLFAFTDEIYEYFLYDGCRHISLATLSGMSERT
                     ITVGGYSKTFSITGWRIGYSIADQRWASLIGAMNDLLYVCAPAPLQAGVASGIKQLPD
                     SFYRDLARDYQMKRDRICHALEKAGLIPSIPQGAYYVLADVSSLPGKTGKERAMHLLN
                     KTGVAGVPGEAFFSGDEGRYFIRFSYAKTDSELDEACRRLARIG"
BASE COUNT          532 a          683 c          630 g          513 t
ORIGIN      
        1 tgccatacaa ggcgtgatgg ccaaacgtct gtgtaagcgg atctgttcca attgcaaaca
       61 gacctaccat ccatcgtccc aagaatacga caaactcgtc cgcgcatacg gcacaaatgc
      121 gtgggaagcg ctaggaatta gctatcacga agaatttcag ctctatcgtg gacatgggtg
      181 cgagacctgc aatcaaacgg gtctcaaagg gcgagttgcg ttgcacgaac tattatgcgg
      241 atcggatgag ttgcggagcc tgattcagaa ccgagccaaa accgctgaaa tgttgagcgt
      301 cgcgattaaa gaaggaatga caacgctcgt acaggacggg attggaaaag tgctcgccgg
      361 cttgactacg tacgaccagg tcagagccgt cgccatgaaa tgattgatct tgcctggtat
      421 cggctatcta ggctcagacg cctgtttcta gcgccttcac aaatctcttc agccgatccc
      481 cctcttcagg acgaaccttg ataaactcaa cgccaaatct cgccggggtc gaccatcgaa
      541 ccgccgccag atcgacacgg accggctcat cccgctcccg gagttgcatc gtcaactgca
      601 tgtaggacgc cggaccggca acggtcgtcg aggtaatggc acagccgtcg gcggacaaat
      661 tcaaaatagt cccttcgccc tctcccgata gctggtctcc attgaacgat acgggaagtt
      721 gaaccgcata ccttatgttt ttacgtgagt ccacgagccc tctgctgcaa accgactgat
      781 cgtcctagat gctccctatc acggagccgg cggtgtctca ctcaccttcg ccgagagaga
      841 ttttctggtg agatgaaacg tgccgccctt ttgagaagga atgtgatcgt caccgatgcg
      901 cgtgtaccac catgtgccat ctccctctga aaagtcagac gaaaactcga cgctgaaccc
      961 gccttcgcgg tcattctcct tttggaacca catccctttc cacgtccggt cggtgagcat
     1021 ctgcgtctca atacgcccgt ccttccaggc gtagggtcca tttccttgtt cgtcgagtgt
     1081 gagcacgaat accgccccat cttcatattc ccattctccg gccaggttgc ggcggtcgct
     1141 cggcagggct ctatccggcg aaggaaccag ttcggttgaa cccacccgtt gaaccgcagg
     1201 atgagtacag gcgctgacac ccaacaccgc acacgaaagc gcgaggagac caatgcgccg
     1261 tacgaacagg ggcagctgta aaccactcat ggttgttgta cttaccatgt ggaagccgga
     1321 tgcacaagca gatgaatcga actgactgtg cggagatgcg cacatcgcgc tgaatcccat
     1381 tttggtgaat ctcttaccca atccgcgcca gtcgccggca ggcttcgtcg agttcggagt
     1441 ccgtctttgc ataactgaac cggatgaagt agcgcccctc gtcccctgag aagaatgctt
     1501 ctccgggcac acccgcgacg ccggtcttgt tcaacaaatg catcgcccgc tctttgccgg
     1561 tttttcctgg caggctcgag acatcggcca atacatagta ggccccttgg gggatcgacg
     1621 gtatgaggcc ggccttctca agcgcgtggc agatgcgatc ccgcttcatt tgataatccc
     1681 ttgcaaggtc tcgataaaac gagtctggga gttgcttgat ccccgatgcc acgccggctt
     1741 ggagaggggc tggtgcacag acatacagca aatcgttcat ggccccgatc aaggaagccc
     1801 acctttgatc cgcgatgcta tatccgatac gccagccggt gatgctgaag gttttggaat
     1861 acccgccgac cgtaatcgtt cgctcgctca tgccggacaa tgtcgcgagg ctgatgtgtc
     1921 ggcaaccgtc atacagaaaa tactcgtaaa tctcatcggt gaacgcgaag agatcgtgcc
     1981 gcctcgtcac atcggctatg ccctcgagct cagtgcgaga aaacaccttc ccggatgggt
     2041 tgcccggtga attcacaata atggccttcg tccgggtggt catcgcgcgt tcaatctcgc
     2101 cggtcgagaa cgtccagtgg ggtgccgtca ttttcacgat gatcggcacc gcttcaacag
     2161 ccaacagtgc actgatgtga tactggtaat agggctcgaa caggatcact tcgtcaccgg
     2221 ggttcagcag ggccaggcaa gcgcagtgaa atgagccggt agctccggaa ctaatcgtga
     2281 tctcggtttc tgggtctgcc ttgatgccgt tgtaccgagc cagctttgat gcgagtgctt
     2341 gccgaagctc cggtaggc
//
LOCUS       DATDNW010000345         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1274029, whole genome shotgun sequence.
ACCESSION   DATDNW010000345 DATDNW010000000
VERSION     DATDNW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274029"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VI359_08300"
     CDS             complement(<1..435)
                     /locus_tag="VI359_08300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6974289.1"
                     /translation="MKLLEGKTAIVTGSSSGIGKAIALRFAGEGAKVVVAARRVKLCE
                     QTVAQIRKAGGEGVVIQTDVMDERQVDTLMQNTMRRYGRIDILVNNAGIFGGRRLAET
                     STAAFDEVMNVNLRGTFFCCRAGFKQMKKQNGGLIINMSSVAG"
     gene            502..705
                     /locus_tag="VI359_08305"
     CDS             502..705
                     /locus_tag="VI359_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974290.1"
                     /translation="MDISHLTPEQLKELVRGFVDDRLRELLGDPDMGLQLGEGLRARL
                     KESLASTERLSGEELADQLGLRW"
     gene            712..975
                     /locus_tag="VI359_08310"
     CDS             712..975
                     /locus_tag="VI359_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY6974291.1"
                     /translation="MAWYRIAFRPTVLKDLAHVDSGMAQRLFDKTKWLASNAENLRHE
                     SLTAELPGLCKYAVGDWRIFYSLDRAEHLVEIHGIVQQRDLRK"
     gene            972..1286
                     /locus_tag="VI359_08315"
     CDS             972..1286
                     /locus_tag="VI359_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974292.1"
                     /translation="MIAVTVSAITRLKAIRVDHPEDPIIRISVRDLDHTRLNFSITLE
                     AAAQPEDAIQEIDGLTIALEQRSVSRMGGVTLDYTTTEGFRFLHNEEGRSLPHLTIPS
                     LN"
     gene            complement(1304..1843)
                     /locus_tag="VI359_08320"
     CDS             complement(1304..1843)
                     /locus_tag="VI359_08320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY6974293.1"
                     /translation="MNYCSVCGKPVSKKIPPGDNLLRFVCESCQTIHYHNPKIVAGCI
                     PEWGDQILLCRRAIEPRSGLWTFPAGFMEIGESTEQAAKRETEEEAHAQVEVTSLYAV
                     LSLPHVGQVYMVFRGRMVTPEFRAGEESLDVRLFTRDAIPWDEIAFSVVKDALHRYVE
                     DAVRGTFRVHVASLPDRLA"
     gene            1875..>2437
                     /locus_tag="VI359_08325"
     CDS             1875..>2437
                     /locus_tag="VI359_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY6974294.1"
                     /translation="MDSTNHRFFTPCPRGLEVVLEQELHGFGISSTKSTEGGIAFQAP
                     WSSLYRVNLESRIASRVLWQVGHAPYRSEDDVYRAAYALPWTDWFLPSHTIKVKVSAR
                     QCPLTSLDFVTLRIKDAICDKFVSVRHRRPSVETHRPHIRIDAFLDATTVTLYLDTSG
                     EPLFKRGHRVDTVEAPLRENLAAGLLR"
BASE COUNT          514 a          772 c          626 g          525 t
ORIGIN      
        1 ccccgcgacg cttgacatat tgatgatcaa tcccccgttt tgctttttca tctgcttaaa
       61 tccggctctg caacagaaga acgttccccg caggttgaca ttcatcacct cgtcgaaagc
      121 cgctgtactc gtctctgcca gacgtctccc accaaagatt ccggcgttgt tcaccagaat
      181 atcgatccgg ccgtatcgcc gcatggtgtt ttgcatgagc gtgtcgactt gacgctcgtc
      241 catgacgtcc gtctgaatga ccacgccctc ccctcccgcc ttgcgaatct gagccacagt
      301 ttgctcacac aatttgaccc gcctggccgc gacaacaacc ttggcccctt caccagcaaa
      361 tcgtaaagcg atcgccttgc caatcccgct gctacttcct gtgacgattg cggtctttcc
      421 ttccagcaac ttcatgaaca gtcctcccta tttcaatgcc ggttgcaatt tagccgcatc
      481 tctccgtatt ctcgtccgat catggatatc tcacacctga cccccgagca gctgaaagag
      541 cttgtgcgcg gcttcgtcga tgaccgactc cgcgagctac tgggcgatcc cgatatggga
      601 ctccaactcg gggaaggcct acgcgcgcgg ctgaaagaat ctcttgctag tactgaacgc
      661 ctgtccggcg aagaacttgc cgaccagctt ggactccggt ggtaactagt catggcttgg
      721 taccgcatcg catttcgtcc gaccgtgctc aaagacctcg cccacgtcga ttccggtatg
      781 gcacaacgac tctttgacaa gacgaaatgg ttggcctcca atgcagagaa cctccgtcat
      841 gaatcgctca cggcagagct gccaggacta tgcaaatacg ccgtcgggga ctggcgcatt
      901 ttctattcgc tcgatcgagc cgaacacttg gttgaaattc acggcatcgt ccaacaacgg
      961 gatttgcgca aatgatcgct gtcacggtct ccgccatcac ccggctgaaa gcgattcgcg
     1021 tcgatcatcc cgaagatccg atcatccgta tcagcgtacg cgatttggac cacacccgct
     1081 tgaacttcag catcaccctg gaagccgccg ctcagcctga ggatgccatt caagagatcg
     1141 acggccttac gatcgccctt gagcaacgca gcgtctcccg gatgggggga gtaaccttgg
     1201 actacaccac caccgaggga tttcgcttcc tgcacaacga ggaagggcgc agcctcccgc
     1261 acctgaccat cccctcgctc aattgatgcg tggacgaatc cggctaggca aggcggtccg
     1321 gcagactcgc cacgtgcacg cgaaacgtgc cgcggactgc gtcctcgaca tagcggtgca
     1381 aggcatcctt tacgacagaa aaagcgattt catcccaggg aatggcgtcg cgggtgaaca
     1441 accgtacatc gagactttcc tctcccgcgc gaaactcggg agtaaccatg cgacctcgaa
     1501 acaccatgta gacctgcccg acatgcggca agctgagcac ggcatacagc gacgtcacct
     1561 cgacctgagc atgcgcttcc tcttcagttt cgcgtttggc agcctgctcg gtgctctcgc
     1621 cgatctccat aaatccggcg gggaacgtcc agaggccgga cctcggctca atcgcgcgac
     1681 ggcacagcag aatctgatcg ccccattccg gaatacagcc cgcgacgatc ttcggattgt
     1741 gatagtgaat cgtctgacag gactcgcaga cgaagcggag caaattgtcg ccgggaggaa
     1801 ttttcttcga taccggtttg ccgcagacgc tgcaatagtt catagctcaa ccgagcctac
     1861 aggacgttct ctagatggat agcacaaacc atcggttctt tacaccttgc ccacgagggc
     1921 ttgaagtcgt gctcgaacag gagctccatg gttttggtat ctcgtctacg aaaagcacgg
     1981 agggtggcat tgcattccag gctccgtggt cctccctgta tcgggtcaac ctcgaaagcc
     2041 gtatcgccag ccgcgtacta tggcaggtcg gccacgcgcc gtatcgttcc gaagacgacg
     2101 tctatcgcgc ggcctatgcc cttccctgga ccgattggtt cctgccgtcg cacacgatca
     2161 aggtcaaggt cagtgcgcga cagtgcccgc tcaccagcct ggatttcgtg accctgcgga
     2221 tcaaggatgc gatttgcgat aaattcgtct cggttcgaca tcgccgaccg agcgtcgaga
     2281 cacatcgccc ccatatccgc atcgatgcgt ttctggatgc gacgaccgtc acgttgtatc
     2341 tcgacacgtc gggtgagccg ctgttcaagc gaggccatcg ggttgacacg gttgaggcac
     2401 ccctgcgcga aaacttggct gccggtctgc tgcgatt
//
LOCUS       DATDNW010000346         7140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_472254, whole genome shotgun sequence.
ACCESSION   DATDNW010000346 DATDNW010000000
VERSION     DATDNW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472254"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VI359_08330"
     CDS             complement(<1..441)
                     /locus_tag="VI359_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974295.1"
                     /translation="MKHREAWLRSVAIINLAFLYLPILVLLVFSFNASRLAATWQGFS
                     WHWYGQLIENGALHAATGNSLLVAVVSTVLAMVLGVPAAMGLERLARRSQHILVGMLL
                     LPLVIPEVMMGVSLLLVFVMAGMPLGLTTVILGHTVFNLPVVIVI"
     gene            complement(438..1304)
                     /locus_tag="VI359_08335"
     CDS             complement(438..1304)
                     /locus_tag="VI359_08335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974296.1"
                     /translation="MTKPVSSHRMTYWLLTPGVLWSVVFLIIPLMVVGLISFATRGTY
                     GGVIWQFSLENYRDLWHVVYARIFGQSLVLASLTTMICLVLGFPLAYYIVRLSPRWQA
                     VWLMLIMIPFWTNFLVRTYAWMFILRTEGLLNTLLLGLGVITTPLDLLYTDKAVLIGL
                     VYGYLPFMVLPLYATLERLDPAFVEAARDLYANQWSVFRRVIVPLTKPGIIAGCLLVF
                     IPSLGAFLTPDLLGGARNMMVGNLIQHEYLVARDWPLGSAISLILLIIVIAAVVAYFF
                     VESTRSRREVEP"
     gene            complement(1340..2437)
                     /locus_tag="VI359_08340"
     CDS             complement(1340..2437)
                     /locus_tag="VI359_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974297.1"
                     /translation="MADPILELVSLVKRHGDVLAVDHVSFAINRGEFFSILGPSGAGK
                     TSVLRLIAGFESPDEGEIRIEGRSISGIPPHRRPVNLVFQSYALFPHLTVGENVAFGP
                     KMQGMAIGDIQSRVRGVLDMVKLTGKERRLPAQLSGGEQQRVAIARALINQPAVLLLD
                     EPLSALDQQLRQEMEGELKAIQKRVGISFVCVTHHQKEALALSDRLAVMHQGRVLQIG
                     TPEEIYGDPDSCFVAQFVGNSNELCGRLIAKEGGSGLFRPDNLDDNHSLQVQVTKDVE
                     IHKPVVCMVRPERLHLSETIERPAFENSLQGRVEKVLYLGSELQYTIQLSRSVQWTVQ
                     VSCLSGGQKVWPVGTTLFVQWRVQDGLVLPL"
     gene            complement(2523..2801)
                     /locus_tag="VI359_08345"
     CDS             complement(2523..2801)
                     /locus_tag="VI359_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974298.1"
                     /translation="MKRDVVVTSLAKVSKPSSNGVKDIAWRLEHLEAQMQKLSELVRE
                     HAEDMDRLVRIVAEDRETIRQQLLRKHHAQVRARKHLRDSDSKVGLDS"
     gene            2933..4171
                     /gene="glgC"
                     /locus_tag="VI359_08350"
     CDS             2933..4171
                     /gene="glgC"
                     /locus_tag="VI359_08350"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY6974299.1"
                     /translation="MNSIFTMILSGGKGERLQPLTEHRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRKVAVLIQYKSHSLDRHIRSGWDILNAELGEYIASVPPQQRISEDWYRGTAD
                     AVYQNLFLLDNEQSEFLLVLAGDHIYKMNYMEMYQWLIAQGADAVVGAIDIPIAEASR
                     FGVMSVDEDFRITGFDEKPVQPTPIPNDPAHAFGSMGIYLFRTKVLREHLLADAQAGT
                     AHDFGKNIIPNMIKSGRVYAFKFHDANKKAVQYWRDIGTLDAYWEANMDLVAVDPLFN
                     LYDQQWPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIVAGGRVQNSVLSPN
                     VRVLDHADVRESIVMENVVIGESSRIKRAIIDKDVVIPPHSEIGYNRDADAQRFTVTE
                     SGLVVISKGMKLHAPVDSSG"
     gene            4146..6005
                     /locus_tag="VI359_08355"
     CDS             4146..6005
                     /locus_tag="VI359_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY6974300.1"
                     /translation="MPPSIHPVDLITALRQKHLTPAIVFLTSRRACDEAMQAFDHDQA
                     LLPPVRQQAIASVLEQVIAQYPSVGEHPLIPTVQRIGVAAHHAGHLPSWKIAVEEMMR
                     HGCLDAVFATTTLAAGVDFPARTVIITQSSIRKSRDFTDLTVAEIQQIAGRAGRRGKD
                     LVGFAIVTPSPYIDLGILTKGLTGQPEAINSQFVITYPMVLNLLKAHPLDQIQPILAK
                     SFAQFQLNQRAEVLEQKLDDLHQKMEPYGPRVCTDWITQWQLFDQFRRHKSHRGPVRR
                     HEPPEVAARFHFLTPGRVIGLSRGRGIVLRQYRSKGQKSPMITVLRPGGGITECPAST
                     VTEVIDRVLDCAEAPVYPWCTPESLDTLTHQLSDLPTRLPLLPILVPKEDEPMPDALA
                     HTFGDFPCPSCPSRSACQKDHAVALKLRQEQHRNTKSIQALRTSLWHRFQERIDVLQR
                     FGYLTATAHLTDIGEWARLIRIDHSLLITELIRAEAFTGADPAILAGIMSSIAHDDDR
                     PGAFPRISTGLASLLGQVRKLAESLAPHEDPPLLRADIAAVTERWIGDPTLTWIGLCK
                     STTMAEGDIYRLLARTLEFLSQLHTLKATHPALAETASRAIAALRRGVLEELP"
     gene            complement(6082..6264)
                     /locus_tag="VI359_08360"
     CDS             complement(6082..6264)
                     /locus_tag="VI359_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974301.1"
                     /translation="MSLSFADEGEDIGFESGAIFSRMTKQQFNQAPLTGSEMPVYPPA
                     SETMQQGDWLLRKQLF"
     gene            6454..7131
                     /locus_tag="VI359_08365"
     CDS             6454..7131
                     /locus_tag="VI359_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY6974302.1"
                     /translation="MAKLGEGDVIVSVPTGSGKTYVAVEAARRAMVENRTVIYTSPLK
                     ALSNTKFTEFSRIFGQDQVGILTGDRRDNGQAPLLIMTTEILRNLLYDAAGGEIDVRL
                     DTLGLVIMDESQYLADPERGVVWEETIIFCPAQARLLLLSASIGNPHDIAEWLTSIRL
                     HPCHLVRHSKRTVPLRAGYLHPNGKLAPLFRTVGIPHGHAGHLHPEAKRLFAQYEDDT
                     LPTRSRR"
BASE COUNT         1620 a         2182 c         1907 g         1431 t
ORIGIN      
        1 gatcacgatg acgacaggga ggttgaagac tgtgtgccca agtatcaccg tcgtcagacc
       61 gagcggcatc ccggccatca caaacaccag cagcaaagac actcccatca tcacttcggg
      121 aatgacaagc ggcagcaaca acatgccgac cagaatgtgt tgagaccggc gcgccagccg
      181 ctcaagtccc atggcagccg gaacacccag caccattgcc aacaccgtcg acaccacggc
      241 cacgagcagg ctattccccg ttgcggcgtg aagcgcgcca ttttcaatga gctggccata
      301 ccagtgccat gaaaatccct gccacgtggc agccaatcgg gatgcattga aggaaaacac
      361 caagagcacg agaatgggca aatacaaaaa tgcgagattg atgatcgcaa ccgaccgcag
      421 ccacgcttct cgatgtttca cggctcaact tccctgcgag accttgtgga ctcaacgaag
      481 aaatacgcga caactgcggc aataacgata attaagagaa tcaaggaaat ggccgatcct
      541 aagggccagt ctcgcgcgac cagatactcg tgctggatca gattgccgac catcatattg
      601 cgcgcaccgc ccaacaggtc tggcgtgagg aaggccccga gagatgggat gaacaccaag
      661 agacagccgg cgatgatgcc gggtttggtg agcggcacga tcactcgccg gaacacggac
      721 cactgattcg catagagatc ccgggccgcc tcgacgaaag ccgggtccag ccgttcgagc
      781 gtcgcataga gcgggagcac catgaagggg agatacccgt acaccagacc gatgagaacc
      841 gccttgtcgg tgtagagcag atcgagcggt gtcgtgatca caccaagacc gagcaatagg
      901 gtgttgagca ggccctcagt tcgcaggatg aacatccagg cataggtgcg gacaagaaag
      961 ttcgtccaga agggaatcat gatcaacatg agccagacgg cctgccagcg gggagacaat
     1021 ctgacgatgt aataggccag ggggaaaccc agcaccagac agatcatggt tgtcagggat
     1081 gcaagaacta gcgactgacc gaagatgcgc gcatagacca catgccacaa atcccgatag
     1141 ttctcgagac tgaactgcca aatcactccg ccgtaggttc cccgcgtagc aaaactgatc
     1201 agccctacca ccatcagagg gatgatcagg aacacaacgg accacaggac tcctggggta
     1261 agcaaccaat acgtcatccg atgactcgac accggcttag tcatgtcccc gagtaactcc
     1321 gcgctacgag agaggtggtt catagcggca gtaccaatcc gtcctggacc cgccattgga
     1381 cgaagagagt cgtcccgacc ggccagacct tctgtccacc ggacagacag gaaacctgca
     1441 ccgtccattg caccgacctc gagagctgaa tggtgtactg cagttcactt cccaaataga
     1501 gcaccttttc gactcgaccc tgcagactgt tctcaaacgc tggcctttca atcgtctccg
     1561 acaggtgcag gcgttctggt cgcaccatgc acacgacagg tttgtgaatt tctacatctt
     1621 tcgtaacctg cacctgaagg ctgtgattgt cgtctagatt gtcagggcgg aacagcccgc
     1681 ttcctccttc tttggcgatc agcctcccgc agagttcgtt cgaattcccc acaaactgtg
     1741 cgacaaaaca gctgtccgga tcgccgtaga tttcttccgg cgtaccgatc tgcagcaccc
     1801 gcccctgatg catcacagca agacgatcgg acaaggccaa ggcttctttc tggtgatgtg
     1861 tcacacagac aaagctgata ccgactcgtt tctgaatggc cttcaactcc ccctccattt
     1921 cctggcgcaa ttgctgatcc aaggccgaaa gcggttcatc caggagaagc acggcgggct
     1981 gattgatcaa ggcacgggcg atggccacgc gttgctgctc tccgcctgat aactgcgccg
     2041 gaagccgcct ctctttcccc gtcagtttca ccatgtccaa tacgccgcgg acacgcgact
     2101 gaatgtcccc gatcgccatc ccctgcatct ttggaccgaa cgccacattc tctcccacgg
     2161 tcaaatgagg aaagagcgca taggactgaa aaaccaaatt gacgggccga cgatgcggtg
     2221 gaatcccgct gatcgatcgc ccttctattc gaatctctcc ctcgtcgggc gactcgaacc
     2281 cggcaatcag gcggaggacg gatgtcttgc cggcaccgct gggtcccaga atcgagaaga
     2341 attcgcctcg gttaatggcg aatgagacgt ggtcgacggc gaggacatcg ccgtggcgtt
     2401 taacgagcga gacgagttcc aggataggat cggccaacgg gacctactcc ggcaggaagg
     2461 aacagcgcgc tatctgatcg gtaatggcgg aaaccaaagc gaggataagc cgacccagaa
     2521 agtcaggaat ctaggccgac tttagaatcg gagtcgcgca ggtgcttgcg tgcacgaacc
     2581 tgcgcgtgat gcttccgtaa gagttgctgg cgaatggtct cccgatcttc agcaacgata
     2641 cggaccaatc gatccatatc ttcggcatgt tcgcggacca actcggaaag tttttgcatc
     2701 tgtgcttcca ggtgctccaa ccgccaggct atgtccttta ctccgttcga cgaaggcttg
     2761 ctgaccttcg ccaacgatgt cacaaccaca tcccgtttca taattcgctc ctcagttcgt
     2821 gttcggtcta atcatgccat ccgactaccc gctagtatca acttgcgtca gcgggatgtc
     2881 aacgttttga ctttctttct atttctaagg ggtcctgata caatttgccg caatgaacag
     2941 tatcttcact atgatcctct cgggcggcaa aggggagcgc ctccagccgc tcacggaaca
     3001 ccgggcgaaa cccgccgttc cgttcggcgg aaaataccgg atcatcgatt ttaccctcag
     3061 caattgcttg aattccggac tccgcaaggt cgccgtgctg atccagtata aatcgcactc
     3121 cctggaccgc catatccgga gcggatggga catactaaac gcggagttgg gcgaatatat
     3181 tgcttcagtg cccccgcaac agcgcatcag cgaagactgg taccgcggta ccgcggatgc
     3241 ggtatatcaa aatcttttcc tcctcgacaa cgagcagtcg gaatttctct tggtcctcgc
     3301 cggcgatcac atttataaga tgaattacat ggaaatgtat cagtggctga ttgcgcaggg
     3361 agctgacgcc gtcgtcggcg ccattgatat tccgattgcg gaggcttcgc gcttcggtgt
     3421 catgagcgtg gatgaagact ttcggatcac agggtttgat gagaaacctg ttcagccaac
     3481 tccgatcccc aatgatcctg cccacgcatt cggttccatg gggatctatc tgtttcgcac
     3541 gaaggttctc cgcgagcatc tcttggccga tgcccaggcc ggcaccgctc atgacttcgg
     3601 gaaaaacatt atcccaaaca tgatcaagag cggacgcgtc tatgccttca agtttcatga
     3661 tgcgaacaag aaagccgttc aatactggcg cgatattgga accttggatg cctactggga
     3721 agccaacatg gatttggttg cggtcgatcc cctgttcaat ctctacgacc agcaatggcc
     3781 gatccgcacc taccaaggtc agtttccgcc cgccaaattc gtgtttgcac aagactttca
     3841 gggcggccgc atgggggtgg cgctggactc catcgtctgc ggcggttgca tcgtcgccgg
     3901 tggccgcgtc cagaactcgg tcttgtcgcc caacgttcgc gtcttggacc acgccgacgt
     3961 gcgggaatca atcgtgatgg agaacgtcgt gatcggcgaa tccagccgga ttaagcgggc
     4021 gatcattgac aaagacgtcg tgatcccgcc gcattccgaa atcggataca atcgggacgc
     4081 cgatgcgcaa cggtttaccg tcaccgaatc cggcctcgtg gtcatttcaa agggaatgaa
     4141 actacatgcc cccgtcgatt catccggttg acctgatcac agcccttcgc cagaagcacc
     4201 tcactccagc gatcgtgttc ctgacatccc ggcgcgcttg cgatgaagcc atgcaggcgt
     4261 tcgatcacga ccaggcgctg ttgcctcccg tcaggcagca ggctatcgct tcggtgctcg
     4321 agcaagtgat cgcgcaatac ccaagcgtcg gagaacatcc cctgattccg accgtgcaac
     4381 gcatcggagt ggccgcccac catgccggcc atctgccatc ctggaaaatc gccgtcgagg
     4441 agatgatgcg ccacggctgt cttgatgcag tctttgccac gaccacgctc gcagccggcg
     4501 tcgactttcc agcccgtacc gtgatcatca cccaatccag cattcgcaag tcgcgcgatt
     4561 tcaccgacct cacggttgct gaaatccaac agattgcagg gcgtgcgggc cgtcgcggga
     4621 aagacctcgt cggattcgcc atcgtgacgc cgtcacccta tatcgatctg ggcatcctca
     4681 ccaaaggctt gactggacag cccgaagcca tcaacagcca gttcgtcatc acctatccga
     4741 tggtgctgaa tctcctgaag gcgcaccccc ttgatcagat ccaaccgatt ctcgcgaaga
     4801 gcttcgcgca atttcagctc aatcaacggg ctgaagtcct ggaacagaaa ctggacgatc
     4861 tccatcagaa gatggaaccg tatggcccgc gtgtctgtac tgactggatc acgcaatggc
     4921 agctcttcga tcaattcaga cggcataaat cgcatcgcgg ccctgtccgg cgccatgagc
     4981 cgcccgaagt cgccgcgcgc ttccactttc tcactccagg acgggtcatc ggactctcac
     5041 gcggccgggg gatcgtcctt cggcagtatc gaagcaaagg tcagaaaagt cccatgatca
     5101 cggttctccg gcccggcggt gggattacgg aatgtcctgc ttcgacggtg acggaagtca
     5161 tcgaccgtgt cttggactgt gcggaagcgc cggtctatcc ctggtgtaca ccggagagcc
     5221 tcgataccct cacgcaccaa ctgagcgatc tgcccaccag gttgccactg ctccctattt
     5281 tagttccgaa agaagatgaa ccgatgccgg atgcgctggc tcacaccttc ggcgattttc
     5341 cttgcccctc atgtccgtcg cgttcggcct gtcagaagga ccacgccgtc gccttgaaac
     5401 tccgccagga acagcaccgc aatacgaagt cgatccaggc cttgcgcacg agtctctggc
     5461 atcgattcca agaacgcatc gatgtcctgc aacggtttgg ttatctgaca gccaccgccc
     5521 acttaacaga tataggggaa tgggcccgtc tcatccgtat cgatcactcg ctgctcatta
     5581 cagagttgat ccgtgctgag gccttcaccg gcgccgatcc cgccattctc gccggcatca
     5641 tgtccagcat cgcgcacgat gacgatcgcc ccggcgcctt tccccgtatc agcaccgggc
     5701 tggcttcgct ccttggacaa gtccgcaagc tggccgaatc cttggcccca cacgaagatc
     5761 caccgctctt acgcgccgac atcgcagccg tcacggaacg atggatcggc gatccgacgc
     5821 tgacgtggat cggcctctgc aagagtacga cgatggcgga aggggatatc tatcgcctat
     5881 tagcacgcac gttggagttt ctctcgcagc tccatacgct caaagccacc catcctgcgc
     5941 tggctgagac agcctctcgt gcgatcgctg cgctgcggcg cggcgtgctg gaggaactac
     6001 cgtgaggaag cactcaacat tcagctctcg gtcgatagct gagtgtcgac agctgaaagc
     6061 taagacatga cgaccgaaga actagaacaa ctgcttgcgc agcaaccagt cgccctgttg
     6121 catcgtctcg ctcgcgggcg gatacacagg catttccgag ccggtaagcg gcgcttgatt
     6181 gaactgctgc ttcgtcattc tgctgaaaat cgcgccggat tcgaatccga tgtcctcacc
     6241 ctcgtcggcg aacgacaggc tcattccgac acatcgatcc ggtcaacctc atcccggcag
     6301 acgccctctc cgcatcattc ccaccggcgt ccgagtcaga aaccgggaga gcctgaaccg
     6361 catgaaccgg ccgtctctct cgctacctgg ctggagggaa tcggagtccc gccgcctcaa
     6421 ccgttcattc cggacgcgtg gcaagacgag gctttggcta aactcggaga aggcgatgtg
     6481 atcgtcagcg tcccgaccgg aagcggaaag acttatgtgg ccgtggaagc tgcccgccgc
     6541 gcgatggtcg agaaccggac ggttatctac acgtcacccc tcaaagcctt gtccaacacg
     6601 aagttcacgg agttctcccg cattttcggc caggaccagg tcggcatctt gactggggat
     6661 cggcgcgaca acgggcaagc gccgcttttg atcatgacga cggagatttt gagaaatctg
     6721 ctttacgatg ccgcaggcgg agaaatcgac gtgcggttgg atacattagg gctggtcatt
     6781 atggacgaat cgcaatatct tgccgatccg gaacgcggcg tggtctggga agagaccatc
     6841 atcttctgtc ccgctcaggc caggttgctc ctgttgtccg cctcgatcgg aaacccgcat
     6901 gacattgcag aatggctgac ctcgatccgc ttacaccctt gccatcttgt acgccacagt
     6961 aaacgcaccg tgccactgcg tgcaggctat ctccatccga acggcaaact ggcgccgttg
     7021 ttccgcacgg tcggtattcc ccatggtcat gcgggccacc tccacccgga ggcgaagcga
     7081 ctcttcgccc agtacgaaga cgataccttg ccgaccaggt ctcggcggta gctgcaccgg
//
LOCUS       DATDNW010000347         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1006055, whole genome shotgun sequence.
ACCESSION   DATDNW010000347 DATDNW010000000
VERSION     DATDNW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006055"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VI359_08370"
     CDS             complement(<1..1653)
                     /locus_tag="VI359_08370"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEY6974303.1"
                     /translation="MEPIITSPDIETRRMELRGIVHLAGEVIAQYWPMRTFVHHNPLH
                     SLEYLPFEETVRRGKQFMGGNGYLPSHQYRAYLKSGRIRIEDLDAALKPLARDTQVLV
                     GSHKLTHGGVLRACLTDGLCDPGVEPLEAELPDPSKELIDQLAAKLTGVLKTRDLRER
                     IGNIVESDQAALGRWLTLSHWCDDTFGTQVVRQINDQLIKWCEAFLDEGHAAWPMPER
                     DKGLYGAWKAIASHEWSPCGIAASGRKIARLPDHPEDALLESLDVLGIPAALRQDYLS
                     LQLTALPGWAGFIKWREEERNYPWQQAYPAGLVKFLAIRLWYARELVQKTCREELGIE
                     GRYDAVIAYMRNSPEEYYLRRQRVAGRLPALYAEEVDRLGHQKRNGWGKVIERYKTEV
                     VPRQQAAARRDMAKRLLALADSFGINPLMLADTPSNDLKQLIEWMEAFPESDHGPVWL
                     KALEVGYQERLLNQMRASFQRPTTGDSPLASPRPYSQSVYCIDVRSEPFRRHLESTGP
                     NETYGFAGFFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIPR"
     gene            complement(1641..2939)
                     /locus_tag="VI359_08375"
     CDS             complement(1641..2939)
                     /locus_tag="VI359_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6974304.1"
                     /translation="MLITWLLSSLPLLGALTSLLFWSNPSRMKLSSVVWSVVSLGSIV
                     GFSNQVVIPPEGLLPLYLLPLTAMISMLAQPAHADHRLSWIMTLVFLGVGLCVVMNQL
                     PISSVALTLLFSGLAVLLYRHHSVLWPMSWWGIGTYALGAICAALSLVATGPLSSVAS
                     VVASAVLLPLMPFHDGHLTALTRLPGNLPSFIVLLLPTIGLHQLATVLPTVPDVAAWT
                     VSLFALVGAFYGAVKALAQTRVRLLLAYASLSFFSMLWWFAAATRSVTPRSALLAGTV
                     GLATSGLLVAWQVVRTRYGDDVDPQAVSGLASGMPRYAVLLSLLALAEMGLPPFGVFA
                     GFMGLLLTAPFPSSAGLVITLLAWLAASWYIMQMVQRLLFGLRRPELRYTDVLHTELA
                     ALSIVVLVLLALGLAPATLFAPNQTGSAAHAVTGSLVWNR"
     gene            complement(2948..>3917)
                     /locus_tag="VI359_08380"
     CDS             complement(2948..>3917)
                     /locus_tag="VI359_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY6974305.1"
                     /translation="SLFAPTPVHALLHAGIINAGGFLINRMAPLFGLSSTTLHVAFVV
                     GTLTAILGASMMLAQNDIKKTLGFSTIGQMGYMIMECGLGAFALAVFHLIAHGLFKAT
                     VFLNCGNVIHKARQEPHFPHVDHRADEEGLSRFTWITGFVTTLLIPLLILLATHGVLH
                     IPLLESQGTVIFLFFIWITSSQAILTLTRLRQVPSWKVSAAMLTTLLFIVFVYLGAAE
                     SFTAFLYPNSDDVTSYFKAASLPDWMFDLIILLATVLTIASWSYLYLIAHGRTVHMPA
                     WIEGLRIRLYVPFMNRLYADEIYEVLGNGIMRLVHQLDKGVQGWSR"
BASE COUNT          879 a         1129 c         1230 g          679 t
ORIGIN      
        1 ccggggaatt tcccgcactt cgttcttggc gcgcatgatg acggggaact gttccgtgtc
       61 atgttccttg ccccaggccc gatagcgaat gaacgcggcg aagaagccgg cgaagccgta
      121 ggtctcgttc ggtccggtcg actcgagatg gcgacgaaac ggctcggatc gtacgtcgat
      181 gcaatagacc gactgcgaat aggggcgtgg agaggccaac ggactgtccc cagtggtcgg
      241 acgctgaaac gaggctctca tctggttcag cagacgttcc tgatagccca cctcaagcgc
      301 cttgagccat accggtccat gatcggactc cgggaacgct tccatccact cgatgagttg
      361 tttcagatcg ttggaagggg tgtcggcgag catcaaggga ttgatgccga atgaatccgc
      421 caacgccaac agtctcttgg ccatatcgcg cctggctgcc gcttgctggc gtggaacaac
      481 ttccgttttg taccgttcga tcactttgcc ccagccgttg cgtttttggt gacctaatcg
      541 atcgacttct tcggcgtaca gagccggcaa gcggccggcg acgcgctgcc gtctcagata
      601 atattcttcc ggagaattcc gcatgtaggc gatgacggca tcgtagcggc cctcgatacc
      661 aagttcttcg cggcacgtct tctggaccaa ctcacgggcg taccagaggc gaatggcgag
      721 gaattttacg aggccggcag gataggcctg ctgccaggga tagttccgtt cctcttcgcg
      781 ccacttgatg aaaccggccc agcctggcag cgcggtcaat tgcagagaca aataatcctg
      841 tcgaagcgct gcgggaatgc cgagcacgtc cagactttca agaagcgcgt cttcaggatg
      901 gtccggaagc cgcgcgatct ttctgccgct ggccgcaatc ccacaaggag accattcgtg
      961 cgaggcaatc gccttccagg caccgtacag tcccttgtct cgttccggca tgggccaggc
     1021 cgcatgccct tcatccagga aggcctcaca ccacttaatc aattgatcgt tgatctgccg
     1081 gacaacctgc gtgccgaacg tgtcatcgca ccagtgcgag agcgtgagcc agcgccccag
     1141 agccgcctga tcgctttcca caatgttgcc gatccgctcg cgcaagtctc gcgtcttgag
     1201 gacgcccgtc agcttggccg caagttggtc gatgagttct tttgaaggat cggggagttc
     1261 ggcttcgagc ggctcgacac ccgggtcgca gagcccgtcg gtcagacagg ctcgcaagac
     1321 cccgccatgc gtcagcttat gcgatccgac gagcacctgt gtgtcgcgcg cgagcggttt
     1381 caatgccgcg tcgagatcct cgatcctgat gcggcccgat ttgaggtagg cccgatattg
     1441 atgactgggg agatagccgt tgcctcccat gaactgtttg ccgcgccgga cggtttcttc
     1501 gaacgggaga tactcgaggc tatggagggg gttatggtgc acgaaggtcc gcattggcca
     1561 atactgcgca atgacttcgc ccgcgaggtg aacgatccca cgcagctcca tgcgtctggt
     1621 ttcgatgtcg ggcgacgtga ttatcggttc catacgagcg atcccgtgac ggcatgagcg
     1681 gccgacccag tctggttggg agcgaagagg gttgccggtg ccaggcccaa cgccagcagg
     1741 accagcacca cgatcgacag cgcagcgagt tcggtgtgca gcacgtcggt atagcgcaac
     1801 tcgggtcgcc gcagaccgaa gagcagccgt tgcaccatct gcatgatgta ccaagacgcc
     1861 gccagccacg cgaggagggt gatgaccagg cctgccgatg atgggaaagg cgcggtcaac
     1921 agcaatccca tgaatccggc aaagacaccg aacggcggca agcccatctc ggccaaagcg
     1981 aggagcgaga gtagcacggc atatcggggc atgcccgacg ccaacccgct gacggcctgg
     2041 ggatccacgt cgtcaccgta tcgagtgcga acgacctgcc atgcaaccaa gagtccgctc
     2101 gtggccaagc cgaccgtccc ggccaatagc gcagaccggg gcgtcactga tcgtgtggca
     2161 gcggcgaacc accacaacat cgaaaagaat gaaagactgg cgtaggccaa caacaagcgc
     2221 acacgggttt gggcgagcgc tttgacggcg ccgtagaagg cccccaccag ggcgaaaagg
     2281 ctcacagtcc aggcggcgac atccgggact gtaggcagta cggtcgcgag ctggtggagg
     2341 ccgatggttg ggagcaagag cacgatgaac gacggcaagt tgcccggcaa tctcgtcaac
     2401 gccgtcaggt gcccgtcgtg gaagggcatg agcggcaaga ggactgcgga ggccaccacc
     2461 gaggcgactg acgaaagcgg acccgtggcc acgagcgaca gggcggcgca gatggcgccg
     2521 agcgcgtacg ttcctatgcc ccaccaggac atgggccaga gcacgctgtg gtggcgatag
     2581 agcaacacag cgagcccgga gaataacaac gtcagggcca cagatgaaat cggaagctgg
     2641 ttcatcacca cgcagaggcc cactccgaga aagaccaagg tcatgatcca cgagagccgg
     2701 tggtcggcat gagctggctg tgccaacatc gaaatcatcg cggtcagcgg aaggagatag
     2761 agcggcaaga ggccttcagg gggaatcacg acctggttgg aaaaccccac aatcgatcca
     2821 aggctgacga ccgaccagac gacggaggag agtttcatcc gggacggatt cgaccagaac
     2881 aggagactgg tcagagcccc cagcaaggga aggctggaca gcaaccaggt gatgagcatg
     2941 ccgtccttca tcgcgaccag ccttgtacgc ctttgtcgag ttgatggacc agccgcatga
     3001 tgccgttccc cagcacctcg taaatctcgt cggcgtagag tcgattcatg aacggcacgt
     3061 acaggcgtat ccgcaatcct tcgatccagg ccggcatgtg caccgtccgg ccgtgggcga
     3121 tcaggtagag ataggaccaa ctggcgatcg tgaggaccgt ggccaggagg atgatgagat
     3181 cgaacatcca atcggggaga gacgccgcct tgaaatacga cgtcacatca tccgaattcg
     3241 gatagagaaa cgcggtgaag gattccgcgg cgcccagata gacgaacacg atgaacaaca
     3301 gcgtcgtaag catcgcggcc gagactttcc aggacgggac ctggcgcaag cgcgtgaggg
     3361 tcaggatggc ttgcgatgac gtaatccaga taaagaacag gaaaatgacc gtaccctgtg
     3421 attcgagcaa tggaatgtgc aagaccccgt gcgtggccaa tagaatcaag agaggaatca
     3481 agagcgtggt tacgaatccg gtaatccagg tgaaccggga caatccctct tcgtcggccc
     3541 gatggtcgac gtgggggaaa tgcggttcct gcctggcctt gtggatgacg ttgccgcaat
     3601 tcaagaacac ggtcgctttg aagagcccgt gcgcgatcag atggaacacg gcgagcgcga
     3661 aggcgccgag gccgcactcc atgatcatgt agcccatctg gccaatcgtc gaaaagccga
     3721 gcgtcttttt gatgtcattc tgcgccagca tcatggacgc gccgaggatc gcggtgaggg
     3781 tgccgacgac gaaggcgaca tgcagcgtcg tggagctcag cccgaagagc ggagccattc
     3841 ggttgatcaa gaacccgccg gcattgatga tccccgcgtg cagcaatgca tggaccggcg
     3901 ttggggcgaa gagcgat
//
LOCUS       DATDNW010000348         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1079428, whole genome shotgun sequence.
ACCESSION   DATDNW010000348 DATDNW010000000
VERSION     DATDNW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079428"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VI359_08385"
     CDS             complement(<1..1604)
                     /locus_tag="VI359_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6974306.1"
                     /translation="MKPIDSDMERRPSQLAREHPSSLAPASRRFISLRTKFVAFFGLI
                     LVLTISALSWYFVETRREAMTDNLQALGTILLTNTVKNNRFTYGGLVPENREALHEFV
                     ESLMAVDHVVYVVITASDGRILDRQSKRTRITSEGSAQPTEQALYPNEQIVKGLLASP
                     VDNPQLTRLVLSREQTLSPQVTTSEWLLPFLVWHETIYDFAIPVLREQEAKSSLPHFS
                     VEQEERVAPSPSPNTPSVLGALQIGITDAEAKRALLIIIRNVLLLTVLLIGAGILGAH
                     VLTLRVTTPLRSLAGAARQLTEGAASPVPLVPTTRDEVGQLTNLFNEMTRSLHERSQA
                     ITANLNTIKRQVSQLTTLHQASAAIASTLNMNQLLDSVLQLLVENLGFSRMVLILRHT
                     EQDAAYVAQITGVSPVIAEAARHLEIPIKDDGGLTADLIIHGKPLLVHDIETISERLH
                     PPVLELLRRSGVRSFVCVPLQSHNQTLGYLAGDRDSQPCTDEDLHILLTIASHVAAAI
                     DNARTYSSLAELTQHLERRIEERTQEL"
     gene            complement(1591..>2073)
                     /locus_tag="VI359_08390"
     CDS             complement(1591..>2073)
                     /locus_tag="VI359_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY6974307.1"
                     /translation="LGILYDPSKSSSRIKDAEPHAAANDFQLQGFPVASEKDVPQQLR
                     ALLATGEALWLMPDSTVLTDESIRFILESALAQHVPVIGFSPEFTRLGALLSLSVNYG
                     EVGRETGLLAKRILDGERILPLKPVPIERLKITVNLKTARFLGIMFPKELNSLIDETY
                     "
BASE COUNT          434 a          523 c          665 g          451 t
ORIGIN      
        1 agttcctggg tgcgttcctc gatacgtcgt tcgagatgtt gggtgagctc cgctagtgaa
       61 gagtaggtcc gggcgttgtc gatggctgcg gccacatggc tggcgatcgt cagcaggata
      121 tgaagatcct cgtccgtaca aggttgcgag tcccggtcgc cggctaaata gccgagcgtt
      181 tggttgtggc tctggagcgg cacgcacaca aacgaccgta cacctgagcg gcgaagaagt
      241 tcgagcactg gaggatgcag ccgctcgctg atagtctcga tgtcatggac gagcaagggc
      301 ttgccgtgga ttatgaggtc ggcggtgagg ccgccatcgt ctttgatggg aatttcgagg
      361 tggcgggccg cttctgcaat gaccggtgag accccggtga tctgggcgac gtacgccgca
      421 tcttgttcgg tatgacggag gatcaacacc atgcgcgaaa agcccaaatt ttcaacaaga
      481 agctgaagca cgctgtcgag gagctgattc atgttgagag tgctggcaat ggcggcgctg
      541 gcctgatgca acgtggtcag ttgactgacc tgtcgtttga tggtgttgag gttggcggtg
      601 atcgcttgac tgcgttcgtg cagcgagcgt gtcatctcgt taaacaaatt cgtcagctga
      661 ccgacttcgt cacgtgttgt cggaacgagg ggaacagggg acgcggcgcc ctcagtcagt
      721 tgccgggccg cgccggcgag gctgcgcagg ggtgtcgtga cccgcaacgt gagcacatga
      781 gcgccgagaa tgccggctcc gatcagcaga acggtcagga gcaagacatt gcgaatgata
      841 atcaagagcg ctcgtttggc ctccgcatca gtgatgccga tttggagcgc ccccaggact
      901 gacggggtat ttggcgaggg actcggagcg actcgttctt cctgctcgac cgaaaaatgc
      961 ggcagagagg acttcgcttc ctgctctcgg aggaccggta ttgcaaaatc gtagatggtt
     1021 tcgtgccaca ctaagaaagg taagagccat tccgacgtag tgacttgtgg actcagggtt
     1081 tgttcgcgtg aaaggaccag acgagtgagc tgaggattgt ctacagggct agccaataac
     1141 cctttgacga tctgctcgtt tgggtagagt gcctgctcgg ttggttgtgc agaaccctcg
     1201 gatgttatgc gcgttcgttt gctttgccgg tccagtatgc ggccgtcaga tgcggtaatg
     1261 accacataca ccacatggtc gaccgccatc agactttcga cgaattcatg aagcgcctcg
     1321 cggttttctg gaacgaggcc tccgtacgta aaacggttat tcttcacggt gttggtgagg
     1381 agaatcgtcc cgagcgcttg cagattgtcg gtcatggcct cacgtctcgt ttcgacgaaa
     1441 taccagctca acgccgaaat ggtaaggacg agaatcaggc cgaagaaggc gacgaacttc
     1501 gtgcgcagac tgatgaacct acggctagcc ggcgccaggg aggatggatg ttcgcgcgcc
     1561 aactgtgacg gtcgtcgttc catatcggaa tcaataggtt tcatcgatga gactgttcag
     1621 ctctttcggg aacatgatgc ccaagaatcg cgccgttttc agattgactg tgatcttcag
     1681 ccgttcgatg ggtacgggtt tgagcggcag gatccgctct ccgtcaagaa tgcgcttcgc
     1741 caacaacccg gtctcccggc cgacctcgcc gtagttgacg gagaggctga gcaaggcgcc
     1801 gagacgtgta aactccggtg agaatccgat gacaggcacg tgctgggcca gcgcggactc
     1861 gaggatgaag cggattgatt catccgtcaa caccgtggag tcaggcatga gccaaagggc
     1921 ctcgcctgtg gcgaggaggg cacgcaattg ctgcggcacg tctttctcgc tcgcaaccgg
     1981 gaatccttga agttgaaagt cgttcgcggc ggcatggggc tccgcatctt tgatccggga
     2041 agaggacttg ctgggatcat agagaatgcc aag
//
LOCUS       DATDNW010000349         6213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_184793, whole genome shotgun sequence.
ACCESSION   DATDNW010000349 DATDNW010000000
VERSION     DATDNW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184793"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VI359_08395"
     CDS             <1..266
                     /locus_tag="VI359_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974308.1"
                     /translation="LIGTLRVGYICRTQCRPSHVRPKMLLMGNHHVDSTLHMIVMVHV
                     YARTRRLSLGRLTCLDQTCAIRENDQAPRKSQNAAEEELKAAC"
     gene            784..1224
                     /locus_tag="VI359_08400"
     CDS             784..1224
                     /locus_tag="VI359_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6974309.1"
                     /translation="MPNHASILLIDDSPGECELFRQALAQARLDIALYTEHDAEAALH
                     FLANRAAHESPPSVILLDWHLEKTRGDQFLTRLRSHGRFASIPVVVFTTSDVSSDMTA
                     GYASGANSYVVKPGTFDELVQCVGVMCRFWTNWNVTPYPVETQC"
     gene            1218..4487
                     /locus_tag="VI359_08405"
     CDS             1218..4487
                     /locus_tag="VI359_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6974310.1"
                     /translation="MLTRAALKNPFAVFAICMIVLILGWVSYQKMKIDIFPDINIPVI
                     LVSTFYRGLSPGEMEGAITLRFEQQFLQASYIEHIESQSLAGMSYIKVFFQPEYSIDA
                     AQSELTSLAYSIIRLLPPGVFPPSVYKFGVASLPIGLLTVSSDTMSEKEMRDLAYFTV
                     RPQMASIPGISTPPVLGGKLRQITVFMDQSKMQGLGISPSEVVKSINAQNAILPAGDV
                     KIGDLDYNLYSNSLIDVVSQINSVPIKVVNGTPVFIRDIGTAADSSAIQTSIVRVNGR
                     ESVYIPVTKQEGANTLAVADAVHERIPKLTEIPAGATVKFLYDQSLYIRQAISNLQKE
                     GLMGAGLAGLMIYLFLRSIKAALVVGLAIPLSLTSALVLLFLSDQSVNIMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSATELTLPILVATICILIVYLPIMFFS
                     GIVKYLFVPLAMTVAFAMSADYLISMSVTPVILAKLYAREQQTGHGQHNEEESGWFGT
                     LVGWYTFLLRLGLRLKPLVVIVVLTALVATFALVAPTLHTEFFPKVDAGNFTMLVTAP
                     EGTRIEKTTAIIGRIESLVKDLIPKEDLDEVISNTGLYYGDAARFAPNTGPHTAFVLV
                     NLVTGHKGETDHYIADLRERLRKDLPGVEVAFQTGGIVSDVLNFGLRAPVDIQVKGPR
                     LDLIRPVAEEIQQRVAQITNTVDVRIKQGKSYPELHLNVDRTKAAYFGLDQQKVVVDV
                     ITGISSNIALSPNYWLDPKTANGYFLLAQYPEQDLTKVEDLLNTPLIGTMVAMGPASA
                     ASRGMTGSTLGLSNTPFANQALALSGGYYNQGTDRQGPSVRLRDVAELIHKTGPDSVD
                     HYDLSRLIDVLVTITGNDLGRVAAKIEDVIKEVRLPKDVVISIKGEVANMRAAFENFA
                     LALPLAVVLIYLVMVGLFRSFIDPLIILLAVPLGWIGTVLMLALTNTSVNVESMIGTL
                     MMMGIVVSNSILLVDFANRMSRSGLPADQAVVEAGRRRIRPIVMTALATILGLLPLAL
                     GFGEGNETMVPLARAVVGGLLVSTVMTLLVVPILHSLVLAREQRTHHRSATPTATSEE
                     I"
     gene            4493..5731
                     /locus_tag="VI359_08410"
     CDS             4493..5731
                     /locus_tag="VI359_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974311.1"
                     /translation="MHGLEDEPGSDNRRYRMLVIVAGIVLLALGTLIYWQRPDAGTPP
                     ATSRPADSEYATGQGSAASSTNAPIDVQVITPTRREIVYSITLPANIAPLYQATLYAK
                     VSGYLKWIGPDKGDWVRKNDVVAIIDAPEVEEQYHQAVADFKIKKLTYERLAKVWAEN
                     PDVIAKQDVDVAQATYEGAKHQMEQRQALRDYTKVRAPFAGIITARFADPGALIQVAT
                     SSATSAIPLFTLMDIETVRIYANVPQEDAHLVKPGSTEAVLIVKELGGRTFSGVVTRS
                     TFALDPSTRSLLVEVDLPNPDHALQPGTFGELALMLNKKPNALVLPPSSIVSTGKGKT
                     VFLVDQGRAKSVSIKTGISDGKWTEITEGLTGGEEVVVVGKSKLTEGAPLAPSPYNLP
                     EGKPSVQRFERPSSGTQPAQ"
     gene            complement(5848..>6213)
                     /locus_tag="VI359_08415"
     CDS             complement(5848..>6213)
                     /locus_tag="VI359_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974312.1"
                     /translation="AQGLSEAKENERVTFYLSQPQTSVKRVITSGGLYIHGTELHFIL
                     GNWQIIYGIPTYGMIYDRRYPMRPTAPKEFDLFFQPSEAVVQYRHSLWDTLLANTTDE
                     LVIDLAKLQQGPIASALSH"
BASE COUNT         1357 a         1865 c         1665 g         1326 t
ORIGIN      
        1 atctgatcgg cacgctgcgc gtcggctaca tttgccggac gcaatgtcgt ccatctcatg
       61 tgaggcccaa gatgctgctg atggggaacc atcatgtcga cagtaccctc catatgatcg
      121 tgatggtcca tgtctacgcc cggaccagga gactctcctt gggccgactc acctgcctgg
      181 accaaacgtg cgctatccgt gaaaacgacc aggctccccg aaaaagccag aatgcagccg
      241 aggaagaact taaagcagcg tgttgatatt agtacgtctt gaccagccat gactcctgtt
      301 cattctatcg tgcgtactga ctcaaagcca gacatcagcc caaataggca ttgcgaagct
      361 cgaacgcagt gggcggcagg tgtagctaga gtaattactc atagagaact tgtttttaac
      421 actatccttt tcccctcccc caatcgcgag ataggtgact tctcacacga agtgtagcct
      481 ggttgatcct gaatgcgcgc gtcgaacagg cacatcgttc ccgctccagt gaaatctgat
      541 gggcagatgt gagcgttccg cgaggaagag gacaactagg ccatactccc acctctctgg
      601 aaaaattcca gtgctcctct ggaatttctc ctcattttcc tctcgattat ctgctcatca
      661 atcacattac cttttcatcg taactggctg tacttcataa gtagttcatc cttgtcgtct
      721 tactcatcga gccaggcaca ctccctgctg atacccctcc tcggattcct gaaggatcgc
      781 cttatgccga atcatgcgtc catcttattg atcgatgaca gccccggcga gtgcgaactg
      841 ttccgccagg cgctcgctca agccaggctc gacatcgcgc tctacaccga gcatgacgcc
      901 gaggcggcgc tgcatttcct ggccaatcgg gccgcgcatg agtcgccgcc ctccgtgatc
      961 ttgctcgatt ggcatttaga aaaaacccgc ggcgaccagt tcctcactcg actccggagc
     1021 cacggtcgct tcgcgtcgat tccggtcgtg gtcttcacga cgtccgatgt ttcatccgac
     1081 atgaccgccg gttatgcctc cggtgccaac agctatgtcg tgaagcccgg caccttcgat
     1141 gaacttgtgc aatgcgtggg ggtgatgtgc cgattctgga ccaattggaa cgtgacgcct
     1201 tatccggttg aaacccaatg ctgacacgag ccgcactcaa gaatccattc gccgtcttcg
     1261 cgatctgcat gatcgtgctg atcctgggct gggtgtcata tcagaagatg aaaatcgaca
     1321 tcttcccgga catcaacatt cccgtcattc tggtctcgac gttttaccgg ggactcagcc
     1381 caggcgaaat ggaaggcgcc attacactcc gatttgaaca gcagtttctg caggcgagct
     1441 atatcgaaca tatcgagtct cagtcgctcg ccggcatgag ttacatcaag gtcttcttcc
     1501 agccggaata cagcatcgac gccgctcaat ccgaactgac cagcctcgca tacagtatta
     1561 ttcggcttct ccctcccggc gtgtttcccc catcggtcta taaattcggc gtggcaagtc
     1621 tgccgatcgg cctgctgaca gtcagcagcg acacgatgag tgagaaggag atgcgggatc
     1681 tcgcctattt caccgttcgt ccacagatgg catccatccc aggaatttcg accccgccag
     1741 tgctcggcgg taagctgcgc cagataacag tcttcatgga ccaatccaag atgcaagggc
     1801 tggggatctc gccgtcggaa gtagtgaaat ccatcaacgc gcagaatgcg attcttcccg
     1861 ccggggacgt gaagatcggc gatctcgact acaacctcta ctcgaacagc ctgatcgacg
     1921 tggtgtccca aatcaatagc gtccccatca aagtcgtgaa cggtaccccg gtcttcatcc
     1981 gcgacatcgg cactgcggcg gactcctccg ccattcaaac ctcgattgtc cgggtgaacg
     2041 gacgagaatc cgtgtacatc ccggtgacga aacaagaagg tgctaacaca ttggcggtcg
     2101 ccgacgccgt gcacgagcgc atcccgaaat tgacagaaat cccagccggc gccacggtta
     2161 aatttctcta cgatcaatcc ctctacattc ggcaggcgat cagcaacttg cagaaggaag
     2221 gtctcatggg tgctggcctt gctggtctga tgatttacct gttcctgcgg agcataaaag
     2281 cggcgctcgt ggtgggattg gccattccgc tttcgctcac ttccgcgctg gtcctgctgt
     2341 ttctctccga ccaaagcgtg aatattatga cgctgggcgg cttggcactc gtgatcggaa
     2401 cgctgctgga caacaacatt gtcgtccaag agaatcttca tcgccatctc gaaatgggca
     2461 aggacggccg gtcggctgcg gaagacagcg ccacggaatt gaccctgccc attctggttg
     2521 ccacgatctg cattctcatc gtgtatcttc cgatcatgtt cttcagcggg atcgtcaagt
     2581 acctgtttgt tcccctcgct atgactgtgg ccttcgctat gagcgccgac tatctcattt
     2641 caatgtctgt cacaccggtg attctcgcga aactgtacgc acgggaacag caaaccgggc
     2701 atgggcaaca caacgaagag gaatccggct ggtttggtac tctggtcggc tggtacacat
     2761 ttctgcttcg cttgggtctg cgcctgaagc cactggtcgt gatcgtggtt ctcaccgcac
     2821 ttgtggcgac ctttgcgctg gtggcaccga cgctccacac agaatttttt cccaaggtcg
     2881 acgccggcaa tttcacgatg ctggtgacgg caccggaggg cacgaggatc gagaaaacaa
     2941 cagcgatcat cggacggatc gaatcattgg tgaaggattt gattcccaaa gaggatctgg
     3001 acgaggtgat ctccaatacc ggcctttact acggagatgc cgcgcgattc gcgccgaaca
     3061 caggccccca taccgcattc gttctcgtga atctggtcac tggtcataag ggagagaccg
     3121 atcactacat cgcagacctc cgcgaacggt tacggaaaga cttgccagga gtggaagtcg
     3181 cctttcagac cggcggcatt gtgagcgacg tgttgaattt tggtctgcgg gctccggtgg
     3241 atattcaggt caaaggcccc cgactcgatc tgatccggcc ggtggctgaa gaaattcaac
     3301 agcgcgtcgc ccaaatcacc aacacggtcg atgtccgcat caagcagggc aaaagctatc
     3361 cggaactcca tcttaacgtg gaccggacca aggccgccta cttcggcctc gatcaacaaa
     3421 aagtcgtcgt ggatgttatc accggcatca gttcaaatat tgcactgagt cccaattatt
     3481 ggctcgatcc caaaaccgcg aatggctatt tcctgctcgc ccaatatcct gagcaggatc
     3541 tgacaaaggt cgaagacctg ctgaacacgc ccctcatcgg caccatggtg gcgatgggcc
     3601 cagccagcgc cgcatcccgc ggcatgaccg gctcgaccct gggactcagc aataccccct
     3661 tcgccaacca ggcattggct ctctccggcg gttactataa tcagggcacg gaccgccagg
     3721 ggccttcggt ccgactgcgc gatgtggccg agttgatcca caaaacaggg ccggactcag
     3781 tcgaccacta cgatctctct cgactgatcg acgtgctcgt gacgatcacg ggcaacgact
     3841 tgggtcgcgt ggcagctaag atcgaagacg tcatcaaaga agtgcgacta cccaaggacg
     3901 tggtcatttc cattaaaggt gaggtagcca acatgcgcgc ggcctttgag aatttcgcgc
     3961 tcgcgctgcc gctggctgtg gtcctgatct atctcgtcat ggtcgggctg ttccgctcat
     4021 tcatcgatcc attgataatt ctgctcgcgg tgcccctcgg ttggatcggc accgtcctca
     4081 tgctggccct gacgaatacg tccgtaaacg tggaatcgat gatcggcaca ctcatgatga
     4141 tgggcatcgt cgtgtccaac agcatcctgc tcgtggattt cgccaaccgc atgtcccgct
     4201 ccggcctccc ggcagatcaa gctgtcgtcg aagctgggcg acgacggatc cggcccatcg
     4261 tcatgacggc gttggccaca atcctcggtc tgctccctct cgcgctcgga ttcggcgagg
     4321 ggaacgaaac gatggtgccg ctggcccgcg ccgtcgtcgg cggactattg gtgtccacgg
     4381 tgatgacgct gttggttgtc cccatcctgc atagtctagt gctggcccgc gaacaacgga
     4441 cgcaccaccg atcggccacc ccgaccgcca catcagagga gatttgatta cgatgcatgg
     4501 tcttgaagac gaaccgggct ccgataaccg acgctaccgc atgctcgtga tcgtcgccgg
     4561 cattgtgtta ctggccctcg gcactttaat ctattggcag cgacccgatg ccggcacgcc
     4621 tcctgccact tctcgtcccg ccgactcgga gtatgcgacc ggccagggga gtgcggcctc
     4681 gagcaccaac gctccgatcg atgttcaggt catcacaccc acccgacggg agatcgtcta
     4741 cagtattacc ctccccgcga acatcgctcc gctctaccaa gcgacgctct atgcgaaagt
     4801 gtccggttat ctgaaatgga tcggtcccga taagggggat tgggtgagga aaaacgacgt
     4861 ggtcgccatc atcgatgccc cggaagtgga agagcagtat caccaagccg tcgccgactt
     4921 caagatcaag aagctcacct acgagagact cgcgaaggtc tgggcagaaa accccgacgt
     4981 gatcgccaag caggatgtgg acgtagctca ggcgacctat gaaggcgcga aacatcagat
     5041 ggaacagcgg caggcgttac gcgattacac caaggtgcga gctccatttg ccggcattat
     5101 caccgcccgg tttgccgatc ctggcgcttt gatccaggtc gcaacgtcgt ctgccacgag
     5161 cgccatcccg ctgttcacgc tgatggatat cgaaaccgtc cgcatctacg ccaacgtccc
     5221 gcaagaggac gcacacctgg tgaaacctgg atcaacggag gcagtcctca tcgtcaagga
     5281 actcggaggg cgaacgttca gcggcgtcgt tacccgcagc acctttgcgc tcgatccttc
     5341 cacccgcagc ctattggtcg aggtcgatct tccaaatccc gatcatgcct tgcagccagg
     5401 tacgttcggc gaactggcgc tgatgctgaa caagaagccg aatgcactcg tcctcccacc
     5461 ctccagcatc gtctcgaccg gcaaaggcaa aaccgtgttc ctcgtcgatc agggacgggc
     5521 gaagagcgtg tcgatcaaaa ccggcatttc ggacggcaag tggaccgaga ttaccgaagg
     5581 ccttacgggc ggcgaagagg tcgtggtggt cggcaagagc aagttaacag agggcgcacc
     5641 gctcgctccg tcaccctaca atctcccgga agggaaaccc tcggtccaac ggttcgaacg
     5701 accctcgtct ggcacccagc cggcacaata ggcaccgcct gcttgagcgc caagctgagg
     5761 cggccggtta caactacata ctttcggaac cgatccgggc cactaccatg gatagtaaac
     5821 caggatagag atagaccagg cgatccgtta gtgcgaaaga gcggaggcga tcggtccctg
     5881 ctggagcttc gcgagatcga tgacgagttc gtcggtggta ttagccagca gcgtatccca
     5941 gagactatgt cgatattgta caacggcctc ggagggctga aagaagagat cgaactcttt
     6001 cggcgccgtc ggcctcatcg gatagcggcg gtcatagatc atgccgtacg ttgggatgcc
     6061 gtagataatc tgccagtttc ccagaatgaa atgcagctcc gtgccgtgga tgtacaaccc
     6121 gcccgaggta atcacgcgct tcacggacgt ttgcggttgg ctcagataga aggtcacccg
     6181 ttcgttctct ttggcctccg acagtccctg cgc
//
LOCUS       DATDNW010000350         3592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_813149, whole genome shotgun sequence.
ACCESSION   DATDNW010000350 DATDNW010000000
VERSION     DATDNW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813149"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VI359_08420"
     CDS             <1..439
                     /locus_tag="VI359_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEY6974313.1"
                     /translation="IEAGVAMSVFFWFLYAHGWTVGQPLDWSTSLYRQATATTFAAIV
                     LTQVANVFACRSERISIFQLGFFSNSRVIWGIAIELVLLLLIVYTPMGNTVFGTDPLP
                     AWIFGPLALGALVLLFAEEARKIITNRYHDRHPFGTARPTSSA"
     gene            526..894
                     /locus_tag="VI359_08425"
     CDS             526..894
                     /locus_tag="VI359_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974314.1"
                     /translation="MRRLTCLLVFVSACAASEPWPAHYLSTGVDRLTQDEIIAQLGPA
                     DETHSLPDGGMEWHYHYRRGRSMVGAHGTMGSSAALECEEYILTFNQAGVLREWIKHM
                     EPTCEPHTQYHPTLESTGRS"
     gene            906..3425
                     /gene="mgtA"
                     /locus_tag="VI359_08430"
     CDS             906..3425
                     /gene="mgtA"
                     /locus_tag="VI359_08430"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEY6974315.1"
                     /translation="MGDDSYWSIPPDELARRVQTTPTGLTSDEARRRQIDYAKIRLRP
                     QRDVNAFLLLLSQFRSPIILILLFAAVISIFLADRTDALIILTIIAVSALLGFWQEYG
                     AAKATASLLALVHVTTEVWRDGKLIEVPSGEIVPGDVVSLSAGSNVPGDGIVLETKDL
                     FVDEATLTGESYPVEKSPTIVATTAPLSKRYNSLFMGTHVVSGDAKALIVRVGKDTEF
                     GRIAHHLKLRPPETEFERGVRRFGYLLLEVTLLLILAIFTINVYLQRPVIESFLFSLA
                     LAVGLTPQLLPAIISVNLSHGAKRMAQRQVIVKRLASIENFGSMNVLCSDKTGTLTEG
                     KMQLHGALSLDGTTSERVLLHAYLNATFETGFLNPLDEAIRSHHTFDVSGYCKLDEVP
                     YDFIRKRLSILVATPATHLLITKGAVEQILTICTLAEQPDGTTVALHEVVDAIRARFH
                     DLSQQGFRVLGLAVRDMGEISDIDKGHEATMTLLGLLVFADRPKAGIAGTIAELRQLG
                     VSLKIVTGDHHLVASHVSREIGLNHPRMVTGRDLRSMTDEALRQRVNAVDVFAEVEPN
                     QKDRIILALKKSGNVVGYIGDGINDAPALHTADVGISVDSAVDVAKDAADIVLLEKDL
                     AVLLDGVREGRVTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNLL
                     TDIPEMTIATDRVDHELIDQPRRWDIAFIRKFMIAFGLVSSVFDYLTFGALLWILQAS
                     TEEFRTGWFVESVISASAIVLVIRTRRTFFTSRPGRYLVSATVAIAAVTLLIPFLPIA
                     RPLGLTPLPPLFLLLLAAILIGYVITAEVVKRRFYAVTRNT"
BASE COUNT          745 a         1069 c          975 g          803 t
ORIGIN      
        1 gatcgaagct ggcgtcgcca tgtcggtctt cttttggttt ctctatgcac atggctggac
       61 cgtgggacaa ccgctggact ggtctacctc gctgtatcgt caagcgactg ccacgacttt
      121 tgctgctatc gttttgactc aggtggccaa tgtattcgca tgccgctccg agcgtatttc
      181 tatcttccag ctcggcttct ttagcaattc tagagtcatt tggggaatcg cgattgagct
      241 cgtgctgttg ctgttgattg tttatacgcc aatggggaac acagtcttcg gaaccgaccc
      301 cttgcctgcc tggatattcg ggccacttgc gctgggtgcc ctggtgctgc tattcgcaga
      361 agaagcccgt aaaatcatca cgaatcggta ccatgacaga catccattcg gaacagctcg
      421 gccaacgagt tcagcatgac agctgacccc aatgaatcca ctcgtcatgt tcccgtcact
      481 gaggttcgac ccacggtgac atctccgaat ggataggagc gtttcatgcg acgactgaca
      541 tgcttgttgg tgttcgtatc ggcctgcgcc gcgagcgagc catggccggc ccattacctt
      601 tctacgggag tagatcggct cacacaggac gagatcatcg cacaattggg gccggcggac
      661 gaaacccact cgttaccgga cgggggcatg gaatggcact atcactaccg tcgaggccgc
      721 tcgatggtcg gtgctcatgg gacgatgggc agttcagccg cgcttgaatg cgaagaatac
      781 attctcacat ttaatcaagc cggggtcttg cgcgagtgga tcaaacatat ggagccgacg
      841 tgtgagccgc acacccagta tcacccaact ctcgaatcga ccgggcggag ctgaggacgt
      901 gtggcatggg tgacgattcg tattggagca ttccgccgga cgaactcgca cgacgcgtac
      961 agacaacacc aacgggcctc accagcgacg aggcacggcg acgccagatt gattacgcca
     1021 agatccggct cagaccgcag cgggatgtca acgcctttct cctcttactc tcacaatttc
     1081 gaagcccgat catcctcatc ctgttattcg ccgcggtcat ttctatcttt cttgcagatc
     1141 gaaccgacgc cctcatcatt ctgaccatca tcgccgtcag cgcgctcctc ggcttctggc
     1201 aagagtatgg agccgccaaa gccaccgcga gtttgctagc gctagttcat gtaacaactg
     1261 aagtctggcg ggacgggaaa ctcatcgagg taccgagtgg ggagatcgtg cctggcgacg
     1321 tcgtaagcct ctccgctggt tccaacgttc ccggagacgg catcgtgctg gaaaccaaag
     1381 acctctttgt cgacgaggcg accctgacgg gtgaatccta tccagtcgaa aaatctccca
     1441 caattgtcgc gaccaccgca cccctttcaa aacgatacaa cagcctcttc atgggcaccc
     1501 atgtggtgag cggcgatgcg aaggcgttga ttgtgcgagt gggaaaagac accgaattcg
     1561 gccgcatcgc ccatcatttg aaactgcgac cacccgagac ggaattcgaa cgaggcgtcc
     1621 gccgcttcgg gtacctgctg ctcgaagtca cgctcctgct catcctggcc atcttcacca
     1681 tcaacgtcta tctccaacgc cccgtgatcg agtccttcct cttttcactg gccctcgccg
     1741 tcgggcttac gcctcaactc ttgccggcca tcatcagtgt gaatttgtcg cacggggcga
     1801 aacgtatggc tcaacgacag gtgatcgtca aacggctcgc gtcgatcgag aatttcggca
     1861 gcatgaacgt cctctgctcg gacaagaccg gcacgctcac ggaagggaaa atgcaacttc
     1921 acggagcttt gagtctggac ggcacaacca gcgagcgcgt gctcctgcac gcctacctca
     1981 acgcgacctt tgaaaccgga tttctgaatc cactggatga agcgattcgc tcacatcaca
     2041 cgttcgatgt gtccggctat tgcaaactcg atgaggtgcc gtacgacttt atccgcaagc
     2101 ggctctccat tctcgttgcc acaccggcca cgcatctcct gatcaccaaa ggggccgtcg
     2161 aacaaatctt gacgatttgc acgctggccg agcaacccga tgggaccacg gtcgcgctgc
     2221 acgaggtcgt tgatgcgatt cgagcccgat ttcacgatct gagccaacaa ggttttcgcg
     2281 tgctcgggct tgctgtacgc gacatggggg agatctcaga catcgataag ggtcacgaag
     2341 cgaccatgac gttgcttggg ctcctcgtct tcgccgatcg accgaaggcc gggattgcgg
     2401 gcaccatcgc ggaactgaga cagctcggcg tgtcgctcaa aatcgtgacc ggagatcatc
     2461 accttgtcgc cagccacgtc agccgagaaa taggactgaa ccacccgcgg atggtcacag
     2521 gacgggatct ccgaagcatg actgatgagg ccctccgcca acgcgtcaat gccgtggatg
     2581 tattcgcgga agtcgagccc aaccagaagg accgtattat tctcgcctta aagaaatccg
     2641 ggaacgtcgt gggctatatc ggcgacggga tcaacgatgc ccccgcgctc catacggcgg
     2701 acgtcggcat ctcagtcgac agcgccgtag acgtcgccaa agatgcggcg gacattgtcc
     2761 tgctcgaaaa agatctggcc gtgcttctcg atggcgtccg ggaagggcgc gttacgtttg
     2821 ccaacacgtt aaagtacgtc ttcatggcga cgagtgccaa cttcggaaac atgttcagca
     2881 tggctggcgc atccctcttc ctgccgtttc ttccgctcct accgaaacaa atcctgctca
     2941 cgaaccttct cactgacatt ccggaaatga cgatcgcgac cgaccgcgtc gatcatgaac
     3001 tcatcgatca gccgaggcgc tgggacatcg ccttcattcg gaagttcatg atcgcttttg
     3061 gccttgtcag ttctgtcttc gattatttga cgtttggcgc gctgctgtgg attctccagg
     3121 cctcgaccga ggaatttcgg actggctggt tcgtggaatc ggtcatctca gcctctgcaa
     3181 tcgtcctggt gattcgaacg agacgtacgt tcttcacgag ccgaccgggg cgctatctgg
     3241 tatcggccac tgtggccatt gctgcggtga ccttgctgat cccttttctg ccgatcgcac
     3301 gacccttggg cttgaccccc ctgcctcctc tgtttcttct cttgttggcg gctatcctga
     3361 tcggatatgt gatcacggct gaagtggtga aacgacgctt ctatgccgtg acgcggaaca
     3421 catgacactg cccgtgcgaa aagcgccacg tggtctttca tccctgatat ttcttgcttc
     3481 tgcatgcggc atcaggcaga accggtcaaa agagcgggtg atggggggcg gaggtggctg
     3541 gtgcggtgcc gggcgcctcg ccggcatttc ctaaaaatct ccatgcgccc tc
//
LOCUS       DATDNW010000351         4102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_473178, whole genome shotgun sequence.
ACCESSION   DATDNW010000351 DATDNW010000000
VERSION     DATDNW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473178"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /gene="glyS"
                     /locus_tag="VI359_08435"
     CDS             complement(<1..1732)
                     /gene="glyS"
                     /locus_tag="VI359_08435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6974316.1"
                     /translation="MGRAKVATSKKHVTSRSSSAAELLVEIGSEELPFDFIAPALASL
                     KEQTERMLAESRLSYRDVHTVGTPRRLVVVVNGLTDHQASTTKETMGPSKAVAFDQTG
                     QPTKAAIGFASGQGVAVEDLQLRQTPRGDYLFAVKREEGQPARDVLTKLVPQIIAKLS
                     FPKSMKWNEAGVRFARPVRWLLALFGGAVLPIEIAGIKAGHNTYGHRILGGGKAVAVR
                     DYSSYVSTLKRVGVIVDQDKRRTLIHEELTHVSQKAGVHLNADEALLEQATYSTEWPV
                     PIVGSFRPEYLSVPSEILITSMKEHQGFFSVRDKKSGALAAHFIAVANNEVKDMSLIR
                     QGNERVLAARLADARFFYDEDRKTALAERATKLSGVVFHQKLGTMAQKQERITKLAGL
                     LAQQLGMSKESAAICSRAASLCKADLLSGIVGEFPELQGVMGGYYAQHDGEPITVSHA
                     IMDHYKPRAMEGDLPRSLEGQVVSMADRIDTIAAFFHVGMVPTGSEDPFALRRHATAI
                     VRIVVEGGLRLDLGQAIEQARTIVAESGFKAVAGSDVQQERLVEFLFERVRYYGRVKH
                     GLRDDVMDAVL"
     gene            complement(1747..2682)
                     /locus_tag="VI359_08440"
     CDS             complement(1747..2682)
                     /locus_tag="VI359_08440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY6974317.1"
                     /translation="MTFQDLILTLHRFWADQGCVIHQPYDLEMGAGTFHPATFLRALG
                     PEPWRSAYAQPCRRPTDGRYGENPNRMQHYYQYQVVLKPSPDNIQELYLESLARLGIN
                     PKQHDIRFMQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIELNPITVELTYG
                     TERIAMYLQQVDNVFDLVWTEGITYGDIHHETEVQFSRYNFEEGDVAMLGATFQAFEA
                     ECKRLLAKKDTRLTLPAYDYCIKTSHMFNLLDAHGAISVAERTGYIARVRALARQCSE
                     RYVEERAAMGHPLNDRPSASSKTERRRTTRRLFQP"
     gene            2953..3387
                     /locus_tag="VI359_08445"
     CDS             2953..3387
                     /locus_tag="VI359_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY6974318.1"
                     /translation="MKLSKKSEYGLRALLELTLAYGKTSLQRHDIADRQHIPVEFLEQ
                     ILLALKRAGLLASQRGMKGGYQLIKRPEDVTVGQVVRILDGPLAPISCVSKTAYQKCR
                     DCPYADKPVCPVQHVMGPVRDAIANILDNYTLNDFAASRREG"
     gene            3390..>4102
                     /locus_tag="VI359_08450"
     CDS             3390..>4102
                     /locus_tag="VI359_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY6974319.1"
                     /translation="MDQVVLVLITFVAATVNGALGYGFSSITVPVALLFYTNRILNPA
                     LVLVELVINSYVLFINRKSIPNIWWRVAPILIGLVIGIGIGSYILFMVQPAWIKFVTY
                     FMLLPLILLQAAGIRKPIRAEKTIAIPFGMGIGTLYSVTTISGPPLALLFNNQGYAKQ
                     DFRAALGVIRVAESSLTAVAYVFLGLYSAGSMEIIPFIVPSVLLGIPLGTYLIRWMDP
                     ETFRRICMAFDALVVAFGLS"
BASE COUNT          887 a         1195 c         1054 g          966 t
ORIGIN      
        1 ctagcacggc atccatcaca tcatcacgca gcccgtgctt taccctccca taatatctga
       61 ctcgctcgaa cagaaactcc accaaccgtt cttgctgaac atccgaccca gccaccgcct
      121 tgaatccact ctcggcgaca atcgtgcggg cctgttcaat ggcctggccc agatcgagcc
      181 ggaggcctcc ttcgaccacg atgcggacga tggcagtcgc atggcgacgc agggcaaacg
      241 gatcttctga tccggtcggc accatgccga catgaaaaaa cgcggcgatc gtatcgatgc
      301 ggtcggccat tgacaccact tgcccttcga gcgaccgggg cagatctccc tccattgcac
      361 gaggtttata gtgatccata atcgcatggc tgacagtgat tggctctcca tcatgttggg
      421 cataatagcc gcccatgacg ccttgcaact cggggaattc ccccacgatg ccggataaca
      481 aatcggcctt acacagactc gcagcccgag aacagatcgc cgctgattcc ttgctcatac
      541 ccaattgctg ggctaacagg ccggcgagct tggttattcg ttcttgcttc tgagccatgg
      601 taccgagctt ctggtgaaac acgacgccgg acagcttcgt cgctcgttcc gcgagcgcgg
      661 tctttcgatc ctcatcgtag aagaatctgg cgtccgcaag acgcgccgcc aagacccgct
      721 cgttcccctg acgaatgagc gacatatcct tcacttcgtt gttagcgacg gcaataaagt
      781 gtgcggctaa ggcaccgctc ttcttgtccc gcaccgaaaa gaacccctga tgttctttca
      841 tcgaagtgat caggatctcc gacgggactg acaaatattc cggtctgaaa ctccccacaa
      901 tgggaaccgg ccattctgtg ctgtaggtcg cttgttccag caacgcttca tctgcattaa
      961 ggtgaacccc ggccttctga ctgacatgtg ttaactcttc gtgaatcaat gtacgccgtt
     1021 tgtcttgatc gacgatcacg ccgacccgct tgagcgtgga tacatacgag gagtaatcac
     1081 ggacggccac tgctttgccc ccaccgagaa tacggtgccc ataggtgttg tggccagctt
     1141 tgatcccggc gatctcgatc ggcagcaccg ccccaccgaa gagggcgagc aaccaccgca
     1201 ccggcctggc gaatcggacc cccgcttcat tccacttcat cgatttcggg aaggacagtt
     1261 tggcaatgat ctgaggcacc aacttcgtca acacgtcacg agccggctgt ccctcttcac
     1321 gtttgactgc gaagagatag tcgcctcttg gggtctgcct gagttgaaga tcttccaccg
     1381 ctacaccctg cccagaagca aatccgatcg ccgccttcgt cggctgaccg gtttgatcaa
     1441 aggcaacggc tttcgacggt cccatcgttt ccttcgtcgt agaagcctgg tggtctgtca
     1501 gtccattcac tacgacgacg agacgccgag gggttcccac cgtgtgcaca tcccgatagg
     1561 acagccgcga ttctgccagc atccgttcgg tctgttcttt gagcgatgcg agggccgggg
     1621 caataaagtc aaaggggagt tcttcgctgc cgatctccac caacagttcg gccgcgctcg
     1681 atgagcgaga cgtcacgtgc ttcttggatg tggcaacctt ggctctgccc atcgatcaga
     1741 tcattcctac ggttggaata ggcgacgggt ggtccgacgg cgttccgttt tcgacgaggc
     1801 gctgggacga tcgtttagcg ggtgccccat ggcggcccgt tcttcgacgt accgttccga
     1861 gcattgccgc gccaacgctc gcactcgtgc tatgtaaccg gttcgctcgg cgacactgat
     1921 ggctccgtgg gcatcgagta gattgaacat gtgcgaggtc ttgatgcagt agtcatacgc
     1981 gggcaacgtc agcctcgtat ctttcttggc cagcaggcgc ttgcactccg cttcgaacgc
     2041 ctggaacgta gcccctaaca tggcaacatc gccctcttca aagttgtagc gggagaactg
     2101 gacttccgtt tcgtgatgga tgtcgccgta ggtgatcccc tccgtccaaa ccagatcgaa
     2161 cacgttatcg acttgctgca gatacatcgc gatccgttcg gttccatagg tcagttcgac
     2221 cgtgatcgga ttgagttcga tccccccgat ctcctgaaag taggtgaact gggtgatttc
     2281 cattccatca agccgtacct cccatcccaa cccccaggct cccaaggtcg gcgattccca
     2341 gtcgtcctgc atgaaccgaa tatcatgctg tttcggattg atacccagcc gggcaagact
     2401 ctcaagatac aactcttgga tgttgtccgg tgacggcttc agcaccacct ggtactggta
     2461 gtaatgctgc atccggtttg gattttctcc ataccgccca tccgttggtc ggcggcaggg
     2521 ttgcgcatag gccgagcgcc agggctctgg accgagcgca cgtaggaagg tcgcgggatg
     2581 aaatgtccca gcccccattt cgagatcgta cggctggtga ataacgcagc cttgatcagc
     2641 ccagaaacga tggagcgtaa ggatcagatc ctggaaggtc acaaacagtc cagtagtcgt
     2701 ccgggcagtt gtggaatagg ctcgtgctaa gacagaaaat gaggtgccac gctagcagga
     2761 atgtctgcag actgtcaagc aacattccct ctatgatcaa tgagttgtgg aagggttgcc
     2821 accgagaatg ttgcacctat tacttccagt aattttcaca cgtaaacatg gaacctcaag
     2881 catagactct tgacggccaa atgatgggta gtctattgtc agaaaacttg attaagtaac
     2941 ttgcatttta ccgtgaagct gtcaaaaaaa agcgaatacg gccttcgggc cctgcttgaa
     3001 ctgaccctcg cctacgggaa aacctcgctt cagcgccatg acattgcaga tcgtcaacac
     3061 attccggttg agtttctcga gcaaatctta ctcgcactga agcgtgctgg cttgcttgcc
     3121 agtcaacggg gaatgaaagg cggctaccag ctgatcaagc gacccgaaga cgtcacggtt
     3181 ggccaagtcg tccgcatctt ggacggtccg cttgcgccga tcagctgtgt gagcaagaca
     3241 gcctatcaaa agtgtcgtga ctgcccctat gcggacaaac cagtctgccc ggtccaacat
     3301 gtgatggggc cggtacgcga cgccatcgcc aatattttgg acaactatac gctcaatgac
     3361 ttcgcagcga gtcgtcgaga agggtaacca tggatcaagt tgtgcttgtc ctaatcacgt
     3421 tcgtcgctgc aacagtaaat ggagcactgg gatacggctt ttcctccatc acggttccag
     3481 tcgcattgct gttttacacc aatcgcatct tgaatccagc cctggtcctc gtcgaactcg
     3541 tgatcaatag ctacgtcctc ttcatcaacc ggaaaagcat cccgaacatt tggtggcgcg
     3601 tcgcaccgat tctcatcggt ctggtgatcg gtattgggat cggtagctat atccttttta
     3661 tggtccaacc ggcctggatc aagtttgtga cctactttat gctactgccg ttgattcttc
     3721 tccaggcggc cggaatcaga aaacctattc gtgctgaaaa aaccatcgcc attccttttg
     3781 gaatgggcat cggcacgcta tattctgtca caaccatttc cggccctccc ctcgcgttac
     3841 tgtttaacaa tcaaggatat gccaagcagg atttccgagc cgcattaggc gttatccgcg
     3901 tggccgaatc cagtctaact gctgtggcct atgtctttct cggactctac agcgctggca
     3961 gtatggagat cattccattc attgttccga gcgtgttgct gggcattcca cttggaacct
     4021 atctgatccg ctggatggat ccggaaacgt ttaggcgaat ctgcatggcc ttcgatgcct
     4081 tagtcgtcgc gtttggatta tc
//
LOCUS       DATDNW010000352         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_327377, whole genome shotgun sequence.
ACCESSION   DATDNW010000352 DATDNW010000000
VERSION     DATDNW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327377"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1047)
                     /locus_tag="VI359_08455"
     CDS             complement(127..1047)
                     /locus_tag="VI359_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6974320.1"
                     /translation="MKRTIAFVVGMAMIGAATLLHAESVADTRNLTLEGAKKVIAATV
                     AEAKRVNAPGGSIAVVDDGGNLLALERLDQTFAASANIAIGKARTAALFKKPTKVFED
                     AIKSGRTSMVTLGGDLPQFTPLQGGIPLEWEGKVVGAIGVSGAASAQQDEELAIAGAK
                     SLESMDIASAAPAHMNGVLPVTYIESQKVAASFQKGAVLVGEDENMMHASRNYMVHAS
                     HRDKAGVVEIHEFDTDIVYVLKGSAQLITGGAPVGTKSIAPHEFRASTVNGGETRKLI
                     PGDVVIIPNGVPHWFKEVEAPFDYYVVKVR"
     gene            complement(1146..1628)
                     /locus_tag="VI359_08460"
     CDS             complement(1146..1628)
                     /locus_tag="VI359_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY6974321.1"
                     /translation="MIRTLALATTIVTLVTGTAQAQLIEKKALTLEGAKRIAAAAEAK
                     AKADGARVVIAVVDESGSLLLLERLDDTQVASVNVGIDKARTAAIFRRPSKVFEDQVR
                     DGRVAALALHGAVPLQGGLPITIEGKVIGAIGVSGETPLQDEDIAKAGAAVAASFTSK
                     "
     gene            complement(1710..2159)
                     /locus_tag="VI359_08465"
     CDS             complement(1710..2159)
                     /locus_tag="VI359_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974322.1"
                     /translation="MSAKGSFTLGMLSMIAAVGLFTAANGLAADAHTTTKFEGVKANS
                     GTATHGRQGNNDTLTWSEEFKIPDTPAPHWQVVDSKGNVFLLQRLKIKGDKENRTITI
                     PSYVHDVAKVQIYCSWAEALLGEASFAKPIVTASGEHTHSDSMAMSR"
     gene            complement(2737..>3109)
                     /locus_tag="VI359_08470"
     CDS             complement(2737..>3109)
                     /locus_tag="VI359_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6974323.1"
                     /translation="ITSLPDQYHTIVGEKGINLSGGQRQRIAIARAILKNPKILLLDE
                     ATSALDSESERLVQEALEQLMKGRTTFVVAHRLTTVQRADRIVVLNKGRVAEVGTHQE
                     LMDQKGLYHYLYTLRLTELPV"
BASE COUNT          566 a          933 c          871 g          739 t
ORIGIN      
        1 tctgccattt ggttcagccc tgtgcgggga gacgagggcc ttggcctcaa ccgcgcgcgt
       61 cgaacgagca cattcttatc gtgcgcgttc tgcgagcgaa gaggcgaggc cctcgtacgc
      121 ctcttcttac cgcaccttta ccacgtaata atcaaacggc gcctccacct ctttgaacca
      181 atgcggcact ccattcggaa tgatcaccac gtcgcccgga atgagtttcc tggtttcgcc
      241 gccgttcacg gtggaggcgc gaaactcatg aggcgcgatc gacttcgtgc cgactggagc
      301 tccacctgta attaactgag cagagccctt gaggacatag acgatgtccg tatcgaactc
      361 gtggatctcc acgacgccag ccttgtcgcg atggctcgca tgcaccatgt agttccggga
      421 tgcatgcatc atgttttcgt cttctcccac gaggacggcg cctttttgaa atgatgccgc
      481 gaccttctga ctttcgatgt atgtgaccgg gagcacgccg ttcatatggg ccggcgccgc
      541 ggacgctatg tccatggact ccagcgactt ggcccccgcg atagccagtt cttcatcctg
      601 ttgcgcgctc gctgccccgc tgacgccgat cgctcccacg actttccctt cccattcgag
      661 agggatgccg ccctgcagcg gcgtaaattg tgggaggtcg ccccctagcg tcaccatcga
      721 ggttcttccg ctcttgatgg catcttcaaa caccttggtg ggtttcttga acaacgctgc
      781 cgtccttgct ttcccgatcg cgatgttggc gctggctgcg aaggtttggt ccagccgctc
      841 cagagccagg agattcccgc cgtcgtcgac gacggcgatc gagcctcccg gtgcgttgac
      901 tctcttggcc tcggcaactg tcgccgcaat cactttcttc gcaccctcca gcgtgagatt
      961 gcgtgtgtcg gccaccgatt cagcgtgtag aagcgtcgcc gctccaatca ttgccattcc
     1021 tacgacgaat gcgattgtcc gtttcatgtc gtttctcctt tcctcagcgt tcaacgttca
     1081 gccatcagtt gtcagcgtcg ccagcagaag tcgaaccctg aaaactgatc gctgcccatt
     1141 gatcgctatt tcgaagtgaa cgatgcggcc acggctgcgc cggctttggc gatatcttca
     1201 tcctgcagcg gcgtttctcc gctaaccccg atcgcgccga tgactttccc ttcgatggtg
     1261 atcggcaagc cgccctgtaa cggcaccgct ccgtgcaatg ccagagctgc tacacgtccg
     1321 tcccgtacct gatcttcaaa aaccttgctc ggccgtcgaa agatcgcggc ggtgcgtgcc
     1381 ttgtcgatcc cgacgttcac actggcgacc tgtgtatcgt ccaaccgctc caacaggagg
     1441 agcgaaccgc tctcatcgac caccgcaatt accacccgcg cgccatcagc cttggctttg
     1501 gcttctgccg ccgcggcgat ccgttttgct ccctccagag tgagggcttt cttctcgatg
     1561 agttgtgcct gtgccgtccc ggtcacaagc gtcactattg tcgttgcaag tgccagagtg
     1621 cgaatcatgg ttttcctcct agttcgacct ccagccgtca gcccccaggc ttctgaaaaa
     1681 tctgagagct gactgctgat ggctggacat caccggctca tagccatgct gtctgaatgc
     1741 gtgtgctcgc ccgatgccgt cacgatgggc ttggcgaaag acgcttcgcc caacaacgct
     1801 tctgcccagg agcaatagat ctgcaccttg gcgacgtcgt gcacatagga cggaatcgtg
     1861 atggtccgat tctccttgtc gcctttaatc ttcagccgct gcagcaaaaa cacattgccc
     1921 ttcgagtcca ccacctgcca atgtggcgct ggggtgtccg gaatcttgaa ctcctccgac
     1981 caggtcaacg tgtcgttgtt gccctgccgg ccgtgcgtgg ctgtgccgct gttggccttc
     2041 acgccttcga acttcgtggt ggtgtgggca tcagccgcca gcccgttcgc cgcggtgaag
     2101 agaccgaccg cagcgatcat ggacagcatt ccgagtgtga acgaaccctt ggcactcatg
     2161 acaatcctcc ttgattatga gttgaacaat ctcgtcgagt tcactcgacg gtgcggattg
     2221 acccgcaatt gcctgttggt ctgaagtgga tgcgagttct tacaaggaag aggtcctgtg
     2281 acggctgatg gagcagggaa aagtcatcgt gtaggtatga ggcagagaca ggtcatccat
     2341 gcaatcggac aactgtttgg ttacgaatgt agcggagagg atgaggtctc gcttgctgag
     2401 aaggttgctc gtcccgcccc gaattgatgg ctgagggagc tgtggtaggt gcagcgatcg
     2461 acgcggcgca atgtgatcgt ccctctgcgg ctgaggcggt caatgacggc gaacagttgg
     2521 ttccatgagt gaccgggcag tctgtggaac agctcttcaa gactcacggg ctcccgaagg
     2581 gacaagcgct cgagtacggc gcattccagc ccttctctgt tcgtcatggt tcctcccatc
     2641 cgctcagagg tattcagctg acgatcggtc gtccgctggg agacaacctt acagaaattc
     2701 ttgagggcga ctggaggggt gctgcagatg atgcggtcag accggcagtt ctgtgagtcg
     2761 gagcgtgtac aggtaatgat acagtccttt ctggtccatc agctcctggt gggtcccgac
     2821 ttccgcgacg cggcctttgt tgagcacgac gatgcggtca gcacgttgta cagtggtcag
     2881 gcggtgggcg acgacaaagg tcgttcgtcc tttcatcaac tgttcaagcg cctcttgcac
     2941 gagtcgctcc gactcgctgt cgagggccga ggtggcttcg tcgagcaata gaatttttgg
     3001 attttttagg atggcccgcg caatggcgat ccgctggcgt tgtccgcctg agaggttgat
     3061 ccccttctct cccacaatcg tgtgatattg atccggcaag cttgttata
//
LOCUS       DATDNW010000353         9663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_391135, whole genome shotgun sequence.
ACCESSION   DATDNW010000353 DATDNW010000000
VERSION     DATDNW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391135"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VI359_08475"
     CDS             complement(<1..701)
                     /locus_tag="VI359_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY6974324.1"
                     /translation="MSHQLQQGNGYTQLAADVLRQAKRSGATEADIIIAEGETFSVQV
                     RLGAVDRLTKAREKQLGLRVFVGKRSASTSTSDFSNKSLDELVTQTCTLAKAVVEDPL
                     SGLPGAEQMAIERPDLDLHDPTKLETDYQIDLARRAESAAMAADLRVTNSEGAEFDSS
                     SGRVVLGNTHGFIGEFWSSSFSLSVSPVATDQQTGSMQRDSWYGVQRKFSKLESPETI
                     GAEAARRTVRRLGAR"
     gene            complement(718..2148)
                     /gene="tldD"
                     /locus_tag="VI359_08480"
     CDS             complement(718..2148)
                     /gene="tldD"
                     /locus_tag="VI359_08480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY6974325.1"
                     /translation="MEELVPLTAFGLSEGEVQAALGRIKVREIDYADLYFESCVSESV
                     SMEESLVKRATKSIAQGVGVRATAGEKTGFAYSDELTRKDLELAADTARYIAQSHTVG
                     QSVPVPVYQRPTRNLYPIEYTQIEVATADRVALLNAIDIEARRYDSRIKNVMASFNTE
                     YKRVVVATSDGMMIGDVQPLSRLQVTCIAEENGNRQVGTFGGGGRIPFEFYQHENRYL
                     AYAREAARQAILNLSAMAAPAGVMPVVLGCGWPGILLHEAIGHGLEADFNRKKTSAFS
                     NLIGKRVASDVCTIVDDGTLPNRRGSLNMDDEGTATSRTVLIERGILRGYITDKLNAR
                     LMGIPLTGNGRRESYQSVVLPRMTNTFMLAGESDPQDIIRSVDHGLYAVSFGGGQVDI
                     TNGKFVFSASEAYLIEGGKVTKPVKGATLIGSGPEILTKVSMVGHDLKLDEGIGTCGK
                     EGQSVPVGVGLPTIRIDEITVGGTQG"
     gene            2365..3150
                     /locus_tag="VI359_08485"
     CDS             2365..3150
                     /locus_tag="VI359_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY6974326.1"
                     /translation="MERRQSSSLKQLSDHELAAKLEPDLFPKHVAVIMDGNGRWAELR
                     GLPRIAGHREGIQSVRELITHCLELGIQALTIYAFSQENWNRPSQEINALMGLLEHYL
                     STERSKLVEQGVRFRAIGRIESLPPSAIQWVRTTEQETAHLDKLHLTVALSYGGRSEI
                     TDAAKRLLRDVQEGNLRPSDIDESRLQQYLYTHDLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRREFLLALLEYQRRDRRFGRVLSALSS"
     gene            3162..4049
                     /locus_tag="VI359_08490"
     CDS             3162..4049
                     /locus_tag="VI359_08490"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY6974327.1"
                     /translation="MEPHISEYRTADHHPTDTTPVATRFDLRRVYTAIALAPLIYAAI
                     RYLPPLAFVALVFVVGATALYEFYRLFFQARTNSVLIGIGLLTLANLLASVYWRGAGM
                     EMLFFGAAAALIIPLVTRENLQHNLIDSAVTLFGVVYIGIALSYMILIRSLPHGESLA
                     LFILLVTWAADTGAYYVGKMFGRHPLAPAISPKKTVEGLLGGLAFAILAVYLCRSWMP
                     SYDLSVMSWILLGSILTITGLIGDLAESALKRSACVKDSSGLLPGHGGMLDRIDSLLF
                     TAPAFYYYVRYSGIPIYGS"
     gene            4074..5234
                     /locus_tag="VI359_08495"
     CDS             4074..5234
                     /locus_tag="VI359_08495"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY6974328.1"
                     /translation="MKTIVILGSTGSIGTSTLDIVERFPDEFRVAGLSAATNDEKLEE
                     QIRRFKPLMVALSNEPAAARLRQRCADLSVEILSGTEGVAQVASISEAEMVISAIVGG
                     AGLVPTLAAIRANKHIALANKEPMVMAGKLMQEEARKHGVRIFPVDSEHSAIFQSLEG
                     HRLEDVRRLILTASGGALWTLTKEELQDVTPERALQHPNWKMGAKITIDSATLMNKGL
                     EVVEARWLFDIPESRIDVLIHRESIIHSLVEYHDRSMIAQLGLPDMRTPISYAMRYPE
                     RMPLDLPSLDLTEIGKLTFCKPDHDRFPCLGLGYESLRVGGTMPAAMNAANEVAVEAF
                     LNGGIRFVDIAEIIRSTMEAHHSREVESLDDALETDRWAREKAEALVHAIAK"
     gene            5304..6701
                     /gene="rseP"
                     /locus_tag="VI359_08500"
     CDS             5304..6701
                     /gene="rseP"
                     /locus_tag="VI359_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY6974329.1"
                     /translation="MLSAFTWSPDTMWLFLQKAWWFLVVLGVLVAFHELGHFLAARWV
                     GVRVLKFSLGFGPKLLGRQIGETEYLLSAIPLGGYVKLFGEDESEAITSEDRRRSFAH
                     QGLWGKVLIVAAGPGFNFILAYLIFAGWLATGAPLFVPTFRDLSPDIEVMVPGSPADK
                     AGMQIGDRVTQVNGKDISTRTELLDAVAKSNGQPLPLTVKRDGRTVPLTVTPIPAPAG
                     HVAAPAEDGTYYLGIEETPPLVTSVLHGSAAAAAGLKAGDHVVSIEGKPIYTWSQMTG
                     IVKENPRRPLHIEVLREGQRVPLTVTPAGELTTVNGQSVEIGKIGISGPGRSLVRSSS
                     PLMSLYSGLEATWGWTELTAIGIYKMIVGDISSKNIGGPLTIANISGEAAAQGPSSVI
                     FLIAILSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQREFAQQVGLVLLVGI
                     MIFAFWNDLERMFSR"
     gene            6708..8435
                     /locus_tag="VI359_08505"
     CDS             6708..8435
                     /locus_tag="VI359_08505"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY6974330.1"
                     /translation="MRTSQLLIPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVIRKVEQIIREEMNRAGAQELLMPVASPAELWKETGRWNFYGKELIRFKDRHER
                     DFCLGPTHEEIITDLFRREVRSYRQLPMNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDKDEAGARISYQKMYDAYCRIFTRCGLKFRPVEADTGLIGGTSSHEFMVLAETGE
                     ETIVYSDTGTYAANVERAEVLPPTEMDTSPLRPLQTVSTPGCRTVEDVTAFLNITPQQ
                     LVKTLLYSTEKETIAVLIRGDHEANEIKIKRLLGVPELELASATTVEQVTGAPVGFAG
                     PVGLHGVRVIADYAVKALRNFVVGGNQNDTHYLNVNWDRDFSAEQFTDLRNARSGDPS
                     PRKDGTLKATKGIEVGHVFMLGTKYSKAMQASYLDPKGQDTLAVMGCYGIGVGRTAAA
                     AIEQNHDDKGIIWPFPIAPFHVHLLALSQSTTTTEAATHLYQELIAAGFEVLWDDRDE
                     RAGVKFNDADLLGAPFQIVIGDKGLAEGSIELKSRKDGVKVKLPPAQIISHLLRIKAD
                     LASTLNQAL"
     gene            8612..>9663
                     /locus_tag="VI359_08510"
     CDS             8612..>9663
                     /locus_tag="VI359_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY6974331.1"
                     /translation="MQAKPIPQNMQELQQKVEFAEHVKRITNQIHAASDLDQILLDLH
                     KDILSLFDAEDLTLFAFDSEKKEIFSKVPHIDTVEEVRIPITEQSLAGFCAKYLRPVN
                     ILDAYNVAELQGIHPSLIHDTSYDKRTGFRTKQVLTYPIVADNKYLMGVLQLLNKKSG
                     GRFTRKDEESVAEIAKALGIAFFNLKKITKKNPTKFDLLVTNNRITQNELDNAIAESR
                     KSISSDLESILIEKYKVPKVDIGKSLAQFHKCPYIEYSDRTIVDVELLKNLNVDYLKK
                     NHWMPLKRDRTAIEILTDDPGDLDRVADIKRTFPGLNIRFAVSLRRDIVQFLNTATGQ
                     ADAGGRKLDENVSDILG"
BASE COUNT         2157 a         2839 c         2494 g         2173 t
ORIGIN      
        1 ttcctggcgc ccaatcgacg gacagttcgc cgggcagctt ctgcgccgat ggtttctgga
       61 ctctccagtt tgctgaattt ccgctgaacc ccataccagg aatcacgttg catcgagcct
      121 gtctgctgat ccgtggcgac cggcgaaaca gacagcgaaa agctcgaact ccaaaattca
      181 ccgatgaagc catgcgtatt gccgagtacc acccggcccg atgaagaatc gaactccgcc
      241 ccctccgagt ttgtcacgcg caaatccgcg gccatggccg cggattcggc ccgtcgcgcc
      301 aaatcgatct gatagtcggt ttccagcttg gtcggatcat gcaggtccag atccggtctc
      361 tcgatcgcca tttgctcagc gccagggaga ccggacaggg ggtcttccac tacggccttc
      421 gccaacgtac aggtttgcgt taccaattca tctaacgatt tattcgaaaa gtctgatgtg
      481 gatgtcgagg cagatcgttt ccccacgaac actcgcaacc ccagctgctt ttctcgagct
      541 ttcgtcaaac gatcgaccgc tcccaggcgg acctgtaccg aaaaggtctc tccctcagca
      601 atgatgatat cggcctcggt tgcaccagac cgcttcgcct gacggagcac atcggcggcc
      661 agttgcgtat agccatttcc ttgttgaagc tgatggctca tcgaatccgc acgcgaatca
      721 tccttgggta ccacccaccg ttatttcgtc gatacggatg gtgggcaatc cgacaccgac
      781 cggcacagat tgtccctctt tcccacaagt cccgatgcct tcatcaagct tcaagtcatg
      841 ccctaccatt gaaaccttgg tcagaatctc cggaccgctg ccaataaggg tcgcgccctt
      901 caccggcttc gtcaccttcc cgccttcgat cagatacgct tcgcttgcgg aaaagacgaa
      961 tttcccgttg gtgatgtcca cctgtccccc gccaaacgac acggcataaa gcccgtgatc
     1021 gaccgagcga ataatatcct gcggatcaga ctctccagcc agcatgaagg tgttcgtcat
     1081 gcgggggagc accacacttt gatagctttc gcggcgtcca ttaccggtca acggaatacc
     1141 catcaaacgc gcgttcaatt tgtcggtaat ataaccgcgg aggattcctc gctcgatcaa
     1201 gaccgttcgg ctcgtcgctg tcccttcgtc atccatgttt aacgagcctc ggcgattcgg
     1261 caacgtgccg tcatctacaa tcgtacaaac atccgaggcg acgcgtttgc cgatcaggtt
     1321 cgaaaaggcg gaggtcttct tccgattgaa gtcggcctcg agcccgtggc cgatggcttc
     1381 atgcaacaag atcccgggcc acccgcatcc cagcaccaca ggcatcacac ctgccggagc
     1441 cgccatggca gacagattga gaattgcctg ccgcgcagcc tcgcgggcat aggctaaata
     1501 ccgattctcg tgctgataaa actcaaatgg aatgcgaccc cctccaccga atgtcccaac
     1561 ctgacgattc ccattttcct cggcaataca ggttacttgg agtctcgaca ggggttggac
     1621 atccccgatc atcatgccat ccgatgtcgc cacgacgacg cgcttatact ccgtattgaa
     1681 cgacgccatc acattcttga tcctcgagtc atagcgtcgc gcttcgatgt cgatcgcatt
     1741 gagcaatgcg acgcggtctg cggttgccac ctcgatttgg gtgtattcaa ttgggtagag
     1801 attcctggtc ggacgctggt agacaggaac gggaacggac tggccgacag tgtgagattg
     1861 tgcgatatag cgagccgtat ccgctgcgag ttccaagtcc ttcctcgtca gctcgtcgga
     1921 ataggcaaac ccggttttct ctcctgccgt ggccctcaca ccaacgcctt gagcaatact
     1981 cttggtcgcc cgtttcacga gcgattcctc catcgagacg gattcggaca cacaggattc
     2041 gaaatagaga tccgcgtaat cgatctcgcg aaccttgatc cgtccgaggg ctgcttgaac
     2101 ttctccctca ctcaaaccga acgcggtcaa cggaaccagt tcttccatga gacaggacac
     2161 cccttctatt caatgattcg atcgctgtaa gaggagggcg gagtatagcc gattcgtttc
     2221 cgcaggcgca atgcgaatgc ttccaacgcc tggctttttc aggagaaaat tcagagatag
     2281 cgtttgactt tcggaacccc caccagtaga ctttgcatac ctgcctatac gacgctcaca
     2341 gaaaggatcc gagtcagact ccctatggaa cggagacagt cctcaagcct taaacagcta
     2401 tccgaccacg aactggctgc caaactcgaa ccggaccttt tcccgaaaca tgtcgccgtc
     2461 atcatggacg ggaacgggcg ctgggctgaa ctccgcggtc tgccgcgcat tgccggtcat
     2521 cgggaaggca ttcagtctgt tcgggagctc attacccatt gtctcgaact tggtatccag
     2581 gctctgacga tctacgcatt ctcgcaggaa aattggaatc gcccgtctca agaaatcaac
     2641 gcgctgatgg gactcttgga gcattatctt tccaccgaac gatccaaact cgttgagcaa
     2701 ggcgtccggt ttcgtgccat cggccgcatc gaatcgcttc ccccatccgc tattcagtgg
     2761 gtccggacca ctgaacagga gaccgcccat ctcgacaagc tccatctgac tgtcgcgctc
     2821 agctatggcg gccgatcaga gatcaccgac gcggcaaaac ggctcctccg cgatgtgcag
     2881 gaaggaaatc tccggccaag cgatattgat gagtcgcggc tgcagcagta cctctatacc
     2941 cacgatctac cggatcccga tctgttgatt cggacaagtg gagaaacccg cattagtaat
     3001 tttcttctct ggcaactcgc ctataccgag ttatatttca ctccaacgct gtggcccgac
     3061 ttccgtcgtc gagaatttct cctcgcgttg ctggaatacc agcggcgtga tcggcggttc
     3121 ggtcgtgtgc tcagcgcgct gtcatcataa tagtctgtac cgtggaaccg cacatttcgg
     3181 agtaccgcac cgccgaccat catccgaccg acactacgcc ggttgcgacg cgatttgacc
     3241 tgcggcgtgt gtatacagcc attgcgctcg caccgctcat ttacgcggcg atccgttatt
     3301 tacctcctct cgcattcgtt gcccttgttt tcgtcgtcgg ggcgaccgca ctctacgaat
     3361 tttaccgtct gttctttcaa gcgcgtacaa acagcgtgct aatcgggatt ggactcctaa
     3421 ccctcgccaa tttactcgca agtgtgtatt ggcgcggtgc gggtatggag atgctgttct
     3481 tcggggccgc ggccgccttg atcattcccc tcgtcactcg cgagaacctg caacacaact
     3541 tgatcgatag cgcggtgact ttgtttggag tcgtctatat cggcatcgcg ctcagctaca
     3601 tgattctcat tcggtctttg ccacatggag aatccttggc cctctttatt cttctcgtca
     3661 cctgggcagc tgatacgggc gcgtattacg tggggaaaat gttcggccgt catcccctgg
     3721 cgcctgccat cagtccgaaa aagacggtcg agggactttt gggtggtctg gcgtttgcca
     3781 ttctcgcagt ctatctctgc cgatcctgga tgccatccta cgacctcagc gtgatgagct
     3841 ggatcctcct agggagcata ctgacgatca ctgggctcat cggcgacctc gctgagtcag
     3901 ctctgaaacg gagcgcctgc gtgaaggatt ccagcggact tcttccggga cacggcggca
     3961 tgctggatcg cattgatagt ctcttgttca cggcgcctgc gttctactac tatgttcgat
     4021 acagcggaat cccgatctat ggatcctagc ggttcgtcac tgggagactg tccatgaaaa
     4081 ccatcgtcat tctcggctcc accggttcga tcggcaccag tacgctggac atcgtggagc
     4141 gcttccccga tgaatttcgc gtggccggtc tcagcgccgc aaccaacgat gagaagttgg
     4201 aagaacagat tcgccggttc aaacctctga tggtggcgct ttcgaacgaa ccagctgctg
     4261 cgcgtctcag gcagcggtgc gctgatcttt cggtcgagat tctgtccggc acggaaggtg
     4321 tggcgcaggt cgcctcgatt tccgaggccg aaatggtcat ctccgccatc gtcggcggag
     4381 cggggctggt ccccacctta gccgcgattc gcgccaacaa acatattgcg ttggccaata
     4441 aagagccgat ggtcatggcc ggcaagttga tgcaggaaga agcccggaaa cacggcgttc
     4501 gcattttccc ggtcgatagc gaacacagtg cgattttcca atctcttgag ggtcatcgac
     4561 tggaagacgt ccgccggctc attctcacgg cttcaggcgg cgcgctctgg actctcacca
     4621 aagaggaact gcaggacgta acgccggagc gcgcgctgca acatcccaat tggaagatgg
     4681 gtgccaagat caccatcgat tctgctacgt tgatgaataa agggctcgaa gttgtcgaag
     4741 cgcgctggct ctttgacatt ccggaatcaa ggatcgacgt cctgatccat cgggaaagca
     4801 tcatccattc tctggtagaa tatcacgacc gatccatgat cgcgcaattg ggactaccgg
     4861 acatgcgaac gccgatctcc tatgccatgc gttaccccga acggatgccg ctggatctgc
     4921 cctcgctaga tctgacggag attggaaagc tgaccttctg taagccggat cacgatcgat
     4981 ttccgtgctt gggcctcggc tatgagtcct tgcgtgtcgg cgggacgatg ccggctgcga
     5041 tgaacgccgc caatgaagtg gcggtcgagg ccttcctgaa cggcggcatt cgattcgttg
     5101 atattgccga gattattcgt tcgaccatgg aggcgcatca ttctcgtgag gtcgaatcgc
     5161 tggatgacgc ccttgaaacg gatcgttggg cgagagagaa ggctgaagcc ctcgtccacg
     5221 cgattgcaaa gtagtgtgac cctcgacgtt ttggcatgaa gtatcttggc ttttcaattc
     5281 aactgtgatt gagggacgtt aacgtgttgt ccgcttttac ctggtctccc gatacgatgt
     5341 ggttatttct ccagaaagcc tggtggttcc ttgtcgttct cggagtcctg gtcgccttcc
     5401 acgaactcgg acactttctg gctgcccggt gggttggtgt ccgcgtcttg aaattctcgc
     5461 tcggcttcgg acctaaatta ctcggccgtc aaatcggtga aacggaatac cttctctctg
     5521 ctattccact cggcggctac gtcaaattgt tcggtgaaga cgaaagtgaa gcgattacct
     5581 cagaggatcg taggcgatcc tttgcgcatc agggattgtg gggcaaggtc ctcatcgtcg
     5641 ccgcggggcc ggggttcaac tttatcctgg cctatttgat ctttgcgggc tggctggcga
     5701 ccggtgctcc cctgttcgtt cccacttttc gagacttgag ccccgatatc gaagtaatgg
     5761 tgccgggatc gcctgccgac aaagccggta tgcagatcgg cgatcgcgtc actcaggtca
     5821 atggcaaaga catctccacg agaactgaac tgctcgatgc ggtggccaag agcaacggac
     5881 agccgctacc ccttacggtg aaacgggatg gtcgaaccgt ccctctcacg gtcacgccga
     5941 tccccgcgcc agcgggacat gttgctgcac cagccgaaga cggcacctat tatctcggta
     6001 tcgaagaaac acctcctctc gtcacctccg ttttgcacgg ttccgcagct gcagcggccg
     6061 gtctcaaagc cggcgatcac gtggtctcga tcgaaggaaa acccatctat acgtggtcgc
     6121 aaatgacggg aatcgtgaaa gagaaccccc gtcgcccgct tcacatagaa gttctgcgtg
     6181 agggccagcg agttccgctg accgtcaccc cagcgggcga actcaccacc gtcaacggtc
     6241 aatcagtcga aatcgggaaa atcgggattt ctgggcctgg ccgttcactc gtgcgctcga
     6301 gcagtccgtt gatgtccctg tacagtggac tcgaagccac gtggggatgg accgagctga
     6361 cagccatcgg catttacaaa atgattgtgg gggacatctc cagcaagaat attggaggcc
     6421 cgctgaccat cgcgaacatc tccggagaag ccgcagccca agggccgtcc agtgtgattt
     6481 tccttatcgc cattctcagc attaacttgg gagtcctaaa cctcttgccc attccaattt
     6541 tggacggtgg tcacctgctg tttttcttaa tcgagggcat actccggaag ccgcttggcg
     6601 aacgccagcg ggaatttgcc caacaagttg ggctggtcct tttggtcggc attatgatct
     6661 tcgccttctg gaacgacctc gagcgtatgt tttcccgctg acatctgatg cgaacctctc
     6721 aactgctcat tcccaccctc cgggaagatc ccggtgaagc tgagaccgtg agtcatcggc
     6781 tcatgctccg cgccggcctc attcgtaagg tagcagccgg catctacaca tatcttccgc
     6841 tcggccttcg cgtgatccga aaggtcgagc aaattatccg cgaggagatg aatcgggccg
     6901 gggctcaaga actgttgatg ccggttgctt caccggctga actgtggaaa gaaaccggtc
     6961 gttggaactt ttacggcaag gagttgattc gattcaaaga ccgacatgaa cgagatttct
     7021 gcttaggccc gacccacgag gaaatcatta ccgatctctt tcgtcgcgag gtccgctcct
     7081 accgacaact ccccatgaat ttctatcaaa ttcagacgaa gttccgcgat gagatccggc
     7141 cccgtttcgg cttgatgcgc ggacgcgaat tcatcatgaa ggacgcctac agcttcgaca
     7201 aggacgaggc cggcgctcga atcagctatc agaagatgta tgacgcgtac tgccgtatct
     7261 ttacccgctg cggcttgaaa tttcgcccgg tcgaagccga caccggcttg atcgggggga
     7321 catcatctca tgagttcatg gtgctggccg aaaccggaga agaaaccatc gtctacagcg
     7381 acactggcac ctatgccgcc aacgttgagc gggccgaagt ccttccgccg accgaaatgg
     7441 acaccagccc cttgcgtcct ctgcaaaccg tctccacgcc gggctgtcgc accgttgaag
     7501 acgtgaccgc ctttctgaac atcaccccgc aacaattggt caagacactc ctctactcga
     7561 ccgaaaaaga aacgattgct gtcttgattc ggggcgatca tgaggcaaac gagattaaga
     7621 tcaagcgact cttgggcgtt cccgaactgg aacttgcgag tgcgacgacg gtcgaacagg
     7681 ttactggggc gccagttggt tttgccggac cagtaggttt acacggcgtg cgggttatcg
     7741 ccgattatgc cgtcaaggcg ttgcgcaatt tcgtcgtcgg ggggaatcag aacgacacac
     7801 actaccttaa cgtcaattgg gaccgtgatt tttcagcgga acaattcacc gacttgcgaa
     7861 atgcccggtc gggcgatccg tcaccgcgga aagacggtac cctcaaagcg acgaaaggta
     7921 tcgaggtcgg acacgtgttt atgctcggga ccaagtacag taaagcgatg caggcctctt
     7981 acctggatcc caaaggtcag gatactcttg cggtcatggg ctgctacggc attggggttg
     8041 ggagaacggc ggccgcggcg atcgaacaaa atcatgacga caagggaatc atctggccct
     8101 ttcccatcgc cccatttcat gtgcaccttc tcgccctgag tcagtcgaca actacgaccg
     8161 aagcagcgac tcacctttac caggaattga tcgctgccgg atttgaggtt ctttgggatg
     8221 accgtgacga acgagccggc gtcaaattca atgatgccga tcttctcggt gcaccattcc
     8281 agattgtgat cggcgataag ggtctcgccg agggctcgat tgaactcaaa tccaggaagg
     8341 acggcgtcaa ggtcaagctg ccgcctgccc aaataatcag ccacctgctt cgcatcaagg
     8401 cggatctcgc ttccacactc aaccaggcct tgtagatcct tgggctctac acctcggaca
     8461 cagtccgtct gaaaacgcct ttctcttgcc ttccacccgg tatcggctag actgacaatc
     8521 aaccttctcg aacagggaat tccagtcata gatgcgctcc acaaggagtc aaagaagcgt
     8581 ctcgcagtca gcgacctgaa ggattcatag catgcaagcc aagcccatcc ctcagaacat
     8641 gcaggaactc cagcagaagg tcgaattcgc ggaacacgtc aagcgcatca cgaatcagat
     8701 ccacgccgcc agtgatttag atcagattct gctcgatttg cacaaggaca tcctcagtct
     8761 gttcgacgcg gaagacttaa ccctgttcgc cttcgactcc gaaaagaaag aaatcttttc
     8821 gaaagtcccg catatcgaca ccgttgagga agtccgcatt cccatcacgg agcaaagcct
     8881 cgcgggattt tgcgcaaaat atctccgccc cgttaatatt ctcgacgctt acaatgttgc
     8941 cgagttacag gggatccatc cctcgctcat tcacgacacc tcctacgaca agcgaaccgg
     9001 ttttaggacc aaacaggtcc taacctatcc gatcgttgcc gacaataaat acttgatggg
     9061 cgtccttcag ctgctcaaca aaaagagcgg cggtcgattt accaggaaag acgaagagtc
     9121 cgtcgcagaa atagccaagg cgctcggcat cgccttcttc aatctcaaaa agatcactaa
     9181 aaagaatccc actaaatttg atctgctcgt cacgaacaac cgtatcactc aaaacgaact
     9241 cgacaatgcc atcgccgaat cacgaaaaag catttcctcc gatcttgaaa gtatcctgat
     9301 cgagaaatac aaggtaccga aagtcgacat cgggaaatct ttggctcagt tccacaaatg
     9361 tccctatatc gaatacagtg atcgaaccat cgttgacgtc gaactcctca agaacctcaa
     9421 cgtcgactac ctcaagaaaa accactggat gcctctcaaa cgggaccgca ccgcaattga
     9481 gatcctgacc gatgaccccg gtgatctgga tcgtgtggcg gacatcaagc gaacgtttcc
     9541 cggtctcaac atccggttcg ccgtaagcct tcgtcgcgac atcgtccaat ttctcaatac
     9601 cgccactggc caagcagatg caggcggacg aaaactggac gaaaacgtat ctgacattct
     9661 cgg
//
LOCUS       DATDNW010000354         2477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1007680, whole genome shotgun sequence.
ACCESSION   DATDNW010000354 DATDNW010000000
VERSION     DATDNW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007680"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VI359_08515"
     CDS             <1..253
                     /locus_tag="VI359_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY6974332.1"
                     /translation="VLGGIDPNTGMVTDIGVLDRVIQEKIGKVFEGQDLRHVLNTQEV
                     TGESLAKAIWDLLVSFISGGKLEKVRLVQTRDLTFEYSE"
     gene            582..1976
                     /locus_tag="VI359_08520"
     CDS             582..1976
                     /locus_tag="VI359_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY6974333.1"
                     /translation="MTSHIHITRSVPVLALLMVVIGSFSDDVQSQIGRFTAHDPGVRS
                     GSAGAGGMIQGLTAFEQQVFGLSREAFEEVQSVQGSIPNTEAGLGPRFNMDSCAGCHA
                     QPDVGGSSPFLNPQVGVAKKEGANNDIPAFVQKDGPIREARFKFNPDGTPDGGVHALF
                     TITGRNDAPGCFLSQPDFRGAAARQNLVFRIPTPVFGAGLIEAIDDDTILANMQANSA
                     TKQSFGIRGRPNMSGRPNTSGNDGTITRFGWKGQNKSLEIFSGEAYNVEQGVTNDLFP
                     QERDEATACYFNTTPESPTHFDAANPLDIPSDVVKFAVFMRFLAPPTPTPDTPSIVQG
                     RKLFSDIGCVLCHTPTLHTSKKAAVGALRDKDANLYSDLMLHNMGPGLADDVSQGNAR
                     GDEFRTAPLWGLGKRIFFLHDGRTKDLLEAIQAHAGGASGQYPPSEANLVIGQFNRLS
                     EIDKQNVLNFLRGL"
BASE COUNT          579 a          656 c          675 g          567 t
ORIGIN      
        1 agtcctcgga ggaattgatc ccaataccgg gatggtgacc gacatcggag tgctcgatcg
       61 tgtgattcag gagaagattg ggaaggtatt cgaagggcag gacttacgac atgtgctcaa
      121 tactcaagaa gtcaccggcg aatcactcgc gaaagccatc tgggatctcc tcgtcagttt
      181 catctcgggt ggaaagctcg aaaaagttcg tctcgtccaa acccgggatc tgacgttcga
      241 gtattcggaa taaacggtta gctcacctat tcttcttctg tccatcagcc cctggtccac
      301 ttgttgccga cgtaggcgac gctttcaaaa caatcagaga agcctgcttc agcacagagt
      361 cccgttctaa tgaattgatg ttcatgctgt agggcactgc cgacgcttcc tccactcaga
      421 ccgcggacag attttgtcct aatctttcca agctacaggt tcacaagcac tttgaactat
      481 cggggcgggt cagcatcgat agggcacggc ttatgctcac ctctcgttgc gcgattgttc
      541 attgatgagc gcgtcgataa ctctactgca tggagggctc catgacctct catatccata
      601 tcacacgctc cgttcctgtg ctggctctac tcatggttgt catcggttca ttttccgacg
      661 acgtccagtc gcagatcggg cgattcaccg cacacgatcc aggcgttcgt tccggctcag
      721 cgggtgctgg aggaatgatt cagggtctga ccgcattcga acaacaggta ttcggattaa
      781 gtcgcgaagc atttgaagaa gtgcaatcgg tgcagggttc gattccgaac acggaagcgg
      841 gcctcgggcc tcgtttcaac atggatagtt gtgccggttg tcacgctcag cccgatgttg
      901 gtggaagcag tccgttcctg aatcctcaag tcggagtggc gaagaaagaa ggggcgaaca
      961 acgacatccc tgcctttgtg caaaaagacg gtcccatacg tgaagcgcgg ttcaaattca
     1021 acccggacgg cacccctgat ggaggagtgc atgccctgtt cacgatcact ggtcggaatg
     1081 atgcgccggg atgtttcttg tcgcaacctg acttccgagg agcagcggca cggcagaacc
     1141 tggtgtttag aattcccact ccagttttcg gtgccggatt gatcgaagcc atcgacgatg
     1201 acacgatcct cgccaatatg caggccaata gtgccacgaa gcagtccttc ggtattcggg
     1261 gccgcccaaa catgagcgga cggccgaata cgagcggcaa tgacggaaca attacgcgtt
     1321 ttggatggaa agggcagaac aagtcgctcg aaatattttc tggcgaggcc tacaacgttg
     1381 agcagggtgt cacgaacgat ctattcccgc aagagcgaga tgaagcgaca gcttgctatt
     1441 tcaataccac gccggaaagt ccgactcact tcgatgctgc gaacccactc gacataccca
     1501 gcgacgtggt aaagttcgcc gtcttcatgc ggtttctcgc tcctccgacg ccgacgccgg
     1561 atactccatc cattgtgcag ggacgaaagc tgtttagcga catcggctgc gttctgtgtc
     1621 acaccccaac gctgcatacg agcaagaagg ctgccgttgg agcattgcgc gacaaggatg
     1681 cgaaccttta ctccgacctg atgcttcata acatggggcc gggtttggct gatgatgtca
     1741 gccagggaaa tgcccgcggc gatgaattcc gcacggcccc cttatggggg cttgggaaaa
     1801 gaatcttctt cttgcacgat gggcgcacga aagatctgct cgaagccatt caggcccatg
     1861 cgggaggagc aagcggtcaa tatccgcctt cagaagccaa tctcgtcatc ggacaattca
     1921 ataggttaag tgaaatcgac aaacagaacg tgctgaactt cttgcgagga ttgtgaccac
     1981 aattcggagt gaggcagatt atttccgcct cactccggct aagccaagaa cgaggtagta
     2041 ccgaagcatc acgtgtgtag gagatcccaa cctcgtctgt gtctatccga gcagattccc
     2101 agtcgttctt gactctgcct cgcgcctatc cgaagagaca aattgagaat gtttcggctg
     2161 gagagtaact taaactctta ctggtcactc cgacgcgggt cgggttggtg gtgtgcgacc
     2221 gggaggaacc ataacaccaa cgagttccta cctgtcaaag ggaataagta aagccgatcg
     2281 ctcggcccac tcttacaatt ctcctagacc tctgtgcaga gacgttgaag gatcttgaat
     2341 aactcggggt aagggtttgc gggattggct ctggtcagcc ggtgcaagct ttcctgtgcc
     2401 tgatcccctg cgtgcgtgca ggggatcagg ctatacgact ggctcttatt gaaccatggg
     2461 aactgtctgg tccgatg
//
LOCUS       DATDNW010000355         3582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_50132,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000355 DATDNW010000000
VERSION     DATDNW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50132"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VI359_08525"
     CDS             <1..545
                     /locus_tag="VI359_08525"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY6974334.1"
                     /translation="WVGGKPHFIRYHDQEWGVPVHSDRKHFEMLLLEGAQAGLTWETI
                     LLRRAGYRKAFAEFDPVKVARFTAKQKASLLKNPGIIRNRLKLESAITNAQAFLAVQR
                     EFGSFDRYIWLFVGGKPKVNRWARSSEVPATSKESDALSKDLKKRGFRFVGSTIIYAY
                     MQAIGLVNDHAADCFLRPRG"
     gene            655..984
                     /locus_tag="VI359_08530"
     CDS             655..984
                     /locus_tag="VI359_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974335.1"
                     /translation="MGMALLLAGELGFAAGTNLAHEKLAAMSEIDRSEVLAIFVEGSG
                     HPCRTVARTFFQGLDSRGNAIWNVECAEGQSYVVEIRNDRDGSTLVINCRTLYAAGGG
                     RCFKRLE"
     gene            1030..2247
                     /locus_tag="VI359_08535"
     CDS             1030..2247
                     /locus_tag="VI359_08535"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6974336.1"
                     /translation="MNAISVRCCIAGGGPAGMMLGLLLVRAGVEVLVLEKHADFLRDF
                     RGDTIHPSTLEVMHELGLLERLLMLPHQKVPRVNGQFGSLALTIAEFSTLSTQCRFIA
                     FMPQWDFLNFLAKEGTRYAAFHLRMQAEVTDLLLEGDRVVGLRATTPDGPLEVRADLT
                     VGADGRHSIVRSRAGLSVEEFGAPMDVLWFRLSRRRDDPEDPMGRFDTGRIFVMLNRG
                     DYWQCGFVIPKGSRAQLQAKGLPAFRTAVADVAPFVADRVGELQDWESIKLLTVQVDR
                     LRQWFRPGLLCIGDAAHAMSPVAGVGINLAIQDAVATANLLAAPLGSGRLTTEDLHQV
                     QRRREWPTHMTQRMQLFIQNRVIKRVLDNAAPLSPPLAFRLLARFPFFSRIPARLIGM
                     GFRPEHVRTPAAP"
     gene            complement(2314..3561)
                     /locus_tag="VI359_08540"
     CDS             complement(2314..3561)
                     /locus_tag="VI359_08540"
                     /inference="COORDINATES: protein motif:HMM:NF023301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HEY6974337.1"
                     /translation="MRRSIGFSIIVISVLSATVSCESTDRMLTGAERAVTSKTGHDII
                     DLASGKDPSQIAKNRLGQYASDPEALLKDIRTAQKDLETLLDALGVKVGEKWGKKEVK
                     LPEQKKYVKYTQNYRSRAIVNFDTGEILVETLDEQSPQTSLKNAIVTTLLTPDDPRSV
                     DLFSDKDITLTGDKEPYLSGLVLDQQGKSIRTPAEAEPFATYLVEKKSSTREVDQGGA
                     KKTAHVVTIRMVSNFSVKQAEKYRPVVQQFAKQYQISPGLVFAIIRTESNFNPYAVSS
                     APAYGLMQLVPSSGGREAYRRAKGKDVTPSRDYLFDPTNNIELGTAYLSVLTFNQLEE
                     VTNTVSREYCVISAYNTGPHNVFRAFSKDTTAALNQINGLQPSAVYEHLRQHLPYQET
                     RHYLEKVTASRKAFVTSSVTASQ"
BASE COUNT          674 a          955 c         1117 g          836 t
ORIGIN      
        1 ggtgggtcgg aggcaaaccg cacttcattc gttatcacga tcaggagtgg ggtgtgccgg
       61 tccattccga tcggaaacat ttcgagatgc tcctgcttga aggggcccag gcagggttga
      121 cgtgggagac gattctgctc cggcgtgcgg gctatcgcaa ggccttcgcg gaattcgatc
      181 cggtgaaggt ggcgcgattt actgcaaagc agaaagcgtc gctcctcaag aatcccggga
      241 tcattcgcaa tcgattgaaa cttgagtcgg ccatcaccaa cgcgcaggca tttctcgccg
      301 tgcagcgtga attcggctcg ttcgaccgct acatctggtt gttcgtcggt gggaagccga
      361 aagtgaaccg atgggcaaga tcctcggagg tccctgccac gagtaaggaa agcgacgcgc
      421 tctccaaaga tctcaagaag cgcggatttc gtttcgtggg cagcacgatc atctatgcct
      481 atatgcaggc catcggactg gtgaacgatc atgcggcgga ttgtttcttg cgcccgcgag
      541 gctgacgtga cgggtgatgc gtgacacgtg aagcgtaatg cgtttgaagt catgaagagc
      601 agaatacccg cgcatcacgc attacggatc acgctttacg agaggatgat ggcgatggga
      661 atggctctgc tgttggccgg tgaacttgga ttcgcggccg ggaccaacct tgcccacgag
      721 aagctggctg ccatgtcgga gattgatcga agcgaggtgc tggccatctt cgtggagggg
      781 agcgggcacc cctgccggac tgtcgcccgc acgttcttcc aaggcctcga ttcaaggggg
      841 aatgcgatct ggaatgtcga atgtgcggaa ggtcagtcct acgtcgtcga gatcaggaac
      901 gatcgcgatg gctcgaccct ggtcatcaat tgccgaacac tgtacgcggc gggtggcggg
      961 agatgtttca aacgactgga ataggctctt catgtttgct cttgcctcga tcctcggagc
     1021 gaccgaatca tgaacgcgat atcggtacgc tgctgcattg cagggggcgg gccggccggg
     1081 atgatgctgg gcctcctgct ggtccgagcg ggcgtcgagg tactggtcct cgagaagcac
     1141 gctgattttc ttcgcgattt ccgcggcgac acgatccatc cgtccactct tgaggtcatg
     1201 catgaactgg ggctgttgga gcggttgctc atgctgccgc accagaaggt gccgcgcgtc
     1261 aatgggcagt tcggcagcct cgccctgacg atcgcggagt tttccactct ctcgacgcag
     1321 tgtcgtttta tcgctttcat gccgcaatgg gattttttga acttcctcgc caaggagggg
     1381 acgcgatatg ccgcctttca tcttcgaatg caggctgaag tgaccgatct tttgctagag
     1441 ggagaccggg tggtgggcct cagagcgacg actccggacg gtccattgga ggtccgcgcc
     1501 gacttgaccg tcggcgcgga cggccgacac tccatcgtcc gttcccgggc gggcttgtcg
     1561 gtcgaagagt tcggcgcgcc catggacgtg ttgtggtttc gactgtcccg tcgccgagac
     1621 gatccggaag atccgatggg tcgcttcgat accggccgga tctttgtgat gctcaatcgg
     1681 ggcgactact ggcaatgtgg cttcgtgatt ccgaaaggat cgcgagccca acttcaggcg
     1741 aagggattgc cggcctttcg tactgccgtc gccgacgtgg cgccgtttgt ggccgatcgc
     1801 gtcggcgaac tccaagactg ggaatcgatc aagttgctta cggtgcaggt cgatcggcta
     1861 cgacaatggt ttcgtccggg gctcctctgt atcggtgatg cggctcatgc catgtccccg
     1921 gtcgctggag tcgggatcaa cttggccatt caggatgcgg tcgctacggc gaatctcttg
     1981 gcggcgccgt taggctccgg ccggctgaca accgaggatt tgcatcaggt gcaacggcgg
     2041 cgcgaatggc cgacccacat gacgcagcgg atgcagctgt tcattcaaaa ccgagtcatc
     2101 aaacgcgtgc tggacaatgc cgccccgctc tcgcctccgc tggcgtttcg tttgctggcg
     2161 cggtttccgt ttttcagccg gattcctgcc cggctcatcg ggatgggctt tcgccccgag
     2221 cacgtgcgaa caccggccgc tccgtaggaa aaggaggatt ccggttggaa cagaggcggc
     2281 ggtcccaaag aaattgacga acgcttctgg acgttactgg ctggcagtta cggatgaagt
     2341 cacgaacgcc ttgcgtgacg ccgtcacctt ttccaagtaa tggcgcgttt cttggtaggg
     2401 caagtgttgt cgtaagtgct catagaccgc gctgggttgc aagccattga tctgatttag
     2461 cgcagcggtg gtatctttcg aaaaggcacg aaagacgttg tgcgggccgg tgttgtacgc
     2521 tgagatgacg caatattcac gggagaccgt gttggttacc tcttccagtt ggttgaaggt
     2581 caatacgctc aagtaggcgg tgcccaattc gatattgtta gtgggatcga ataggtagtc
     2641 tcgcgacggg gtgacatcct tgcccttggc acgccggtag gcttctcgcc cgccgctcga
     2701 ggggacgagc tgcatcagcc catacgccgg cgcggaactg acggcgtagg gattgaagtt
     2761 gctctccgta cgaatgatcg caaagaccag ccccggactg atttggtatt gtttcgcaaa
     2821 ctgctgaacc accggccggt atttctccgc ctgcttcacc gagaaattcg agaccattcg
     2881 gatcgtgaca acgtgagcgg ttttctttgc cccgccctgg tcgacctctc gcgtgctgga
     2941 tttcttttcg acaagatagg tggcaaacgg ttcggcctcc gccggtgtac ggatggattt
     3001 gccctgttga tcgagcacga ggcccgagag gtagggttct ttgtcgccgg tgagagtgat
     3061 gtctttgtcg gaaaacaggt cgacggaacg aggatcatcc ggggtgagga gggttgtcac
     3121 gatggcattc ttgagactgg tctgcggaga ttgctcgtcg agggtttcga ccagaatctc
     3181 acccgtgtca aagttcacga ttgcccggct ccggtaattt tgcgtgtact tcacatactt
     3241 cttctgctcg ggtaatttga cttccttctt tccccatttc tcgccgactt tcactcccag
     3301 cgcgtccagg agcgtctcca agtctttttg ggccgtgcgg atgtctttca gcagggcttc
     3361 aggatcgctt gcgtattgac cgagccgatt cttagcgatc tgggagggat ctttgcccga
     3421 agctaagtcg atgatgtcat ggccagtctt gctcgtgacc gctcgttcag ctccagtgag
     3481 cattcggtcc gttgactcgc agctgactgt tgcagacaga acactgatga cgatgatgga
     3541 gaagccgata gaacgtcgca tggatttatt ctacaggatt tg
//
LOCUS       DATDNW010000356         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_50256,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000356 DATDNW010000000
VERSION     DATDNW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50256"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VI359_08545"
     CDS             complement(<1..449)
                     /locus_tag="VI359_08545"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEY6974338.1"
                     /translation="MKPTMCRPNNLVRVCGAVGVVGLLTLTPPIGAQATPSSDETATV
                     VDRVATIEPRSIEELPAQALTDIELRREQNAITVVISGDGKISHEARLIDERRLVVDI
                     PLVVSALPKPVMVVHHPILTRVRLGYHPDKVRVVMDLASPATFSVEP"
     gene            complement(446..997)
                     /locus_tag="VI359_08550"
     CDS             complement(446..997)
                     /locus_tag="VI359_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY6974339.1"
                     /translation="MLGEPVGAGTDLIATMKQIGPMRQDSVKAPAVTDVGRQTPSSAV
                     AASVGDSSVGVSEGAPSPESAATPRSYDPSGRRDPFAPIVQQFGLGPVDQTLPPLQRV
                     GLTEMNLIAIVWGSYGYTAMVQTPDGNGYAVRRGTRIGPNNGVVSAITEKGIIVQERF
                     TDVYGKKQEREYVKLLHPKEGVE"
     gene            complement(1111..1737)
                     /gene="pilO"
                     /locus_tag="VI359_08555"
     CDS             complement(1111..1737)
                     /gene="pilO"
                     /locus_tag="VI359_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY6974340.1"
                     /translation="MKLPELNIEVLRTIPLLQKVGLLALVLAAILVGFYYQIVEPKSA
                     EIEGLQGAIAKLDTEIQTLTIKVKHLDELIAASKQLEIELAKKKERLPPEEEAVMLLK
                     QVSDLGVRLGLDIRLWRPGAKTEDASKLFVRMPVNVEVSGGYHTAAIFFDRINSLPRI
                     VTVSGLKMGSPKIEKGRVVTQTVFDLIAYAAPQETKVAAAVPAAAPKK"
     gene            complement(1744..>2140)
                     /locus_tag="VI359_08560"
     CDS             complement(1744..>2140)
                     /locus_tag="VI359_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY6974341.1"
                     /translation="KQAEKQDKEKQVAQLKEQVKQVADFEQRKKILEDKNRIIDQLEK
                     SRVGPVKVLDHVSQSLEPLKLWLVRVGVNGQSIELEGRAMTNDDVVEFVNNLRRTDYF
                     MNIDLQESKAATESQVNVYQFKLNFRLKS"
BASE COUNT          471 a          636 c          485 g          548 t
ORIGIN      
        1 tgaggttcga cagaaaatgt ggcggggctc gccaaatcca tgacaacccg aactttgtct
       61 ggatgatagc cgaggcggac cctcgtcaaa atcggatgat gaaccaccat caccggcttc
      121 ggcaatgcag agaccactag cggaatatcc acgacaagcc ggcgctcatc gatcagtctt
      181 gcttcatgag aaatctttcc atctcctgag atcaccaccg taatcgcgtt ttgttcgcgg
      241 cggagctcaa tgtcggtcaa cgcttgggca ggcaattcct cgattgacct tggctcaatc
      301 gtggctacac gatccacaac cgtggctgtt tcgtcgctcg agggagtcgc ttgagctcca
      361 atgggcggcg tcagcgtcag gaggcctaca acccccacag ccccacacac acgaaccaga
      421 ttgtttggtc tgcacatcgt tggtttcatt ctacgccctc tttcggatgg aggagcttga
      481 catattctcg ctcctgtttt ttcccataga catccgtgaa ccgctcttga actataatgc
      541 ccttctctgt aattgcactc acaaccccgt tattgggacc gattcgtgtt ccacgccgca
      601 cggcatagcc attaccgtct ggcgtctgaa ccatcgcggt atatccgtag gacccccaca
      661 caatggcaat gagattcatc tctgtcagac cgactcgttg caatggaggc agggtttgat
      721 cgactggacc gaggccgaat tgctggacga tgggagcgaa cggatctcga cgaccagacg
      781 gatcatacga gcggggtgtg gccgccgact cgggagatgg cgcaccttca ctgaccccca
      841 ccgatgaatc tccaacagac gctgccaccg ctgacgaagg tgtctgtctc ccaacgtcgg
      901 tcacagcggg cgccttgacg gaatcttgtc tcatgggccc aatctgcttc atcgtcgcaa
      961 tcagatctgt accggcaccc acaggttccc cgagcatgat caacgctacc atccccactg
     1021 tactaattcc tgtccgaatc cttgtcatcg ctcttcgcct tcgctctctt ctgataaccg
     1081 taaccacgga tgacagcatt ctttctctac ttacttcttt ggtgccgcag ccggcactgc
     1141 cgcagcaacc ttggtttcct gtggcgccgc gtacgcgatg agatcaaaca cggtttgcgt
     1201 taccactcgt cccttttcaa ttttcggcga tcccattttc aatccagaca cggtcacgat
     1261 tcgaggaaga ctgttgatac gatcgaagaa aatcgccgca gtgtgataac ctccggacac
     1321 ttcgacattc accggcatac ggacgaacag cttggaggca tcttctgtct tggcacccgg
     1381 cctccacagc ctgatatcca ggcccaaccg cacacccaag tcagatacct gtttcagaag
     1441 cattaccgct tcctcttccg gtgggaggcg ttctttcttc ttggccagtt caatctctag
     1501 ctgtttgctc gcagcaatca gctcatccag atgtttcacc ttgatggtca acgtctgaat
     1561 ttcagtgtcc agctttgcaa tggctccctg aagtccttca atctctgcag acttgggctc
     1621 aacaatttga tagtaaaacc cgaccaggat cgcggcaagc acgagcgcaa gaagccctac
     1681 tttctgcagc aacggaatag tgcgaagaac ttcgatattg agctctggaa gtttcatggc
     1741 aacttaactt ttcagacgga aattaagttt gaactgatag acattgactt gactctctgt
     1801 cgcagcctta ctctcctgaa gatcgatatt catgaaatag tctgtgcgac gaaggttatt
     1861 gacgaattcc accacatcgt cgttcgtcat cgcacgaccc tcaagctcaa tactttgtcc
     1921 gtttactccc actcgcacca accacagctt taacggctcc aaactctggc tgacatgatc
     1981 caacactttc accggaccta cgcgtgattt ctccagctgg tcgatgatgc gattcttatc
     2041 ttccaatatc ttcttgcgtt gttcgaagtc cgcaacttgc ttgacctgtt ctttgagctg
     2101 tgcgacctgc ttttccttat cctgtttttc cgcctgtttg
//
LOCUS       DATDNW010000357         4901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_393249, whole genome shotgun sequence.
ACCESSION   DATDNW010000357 DATDNW010000000
VERSION     DATDNW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393249"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VI359_08565"
     CDS             <1..318
                     /locus_tag="VI359_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974342.1"
                     /translation="AKYEKGKTQYLCPAPLSAAITKQIRTLALKTYQVMGCDGAIRVD
                     FRITPRGRPYVLEINTVPGMTETSLLPMAAAQAGIDYDELTERILQSALVRARERVAS
                     TRG"
     gene            323..1189
                     /locus_tag="VI359_08570"
     CDS             323..1189
                     /locus_tag="VI359_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY6974343.1"
                     /translation="MMWLRKRPRRAAGPRQNQWKGPRATLSVHQRQQAHRARRWARWR
                     RVLLASGWLAGGAVAVWSLMLAAGEIAPILQRGLEIREVKVTGVRHMTQQDVLDRLAL
                     KKGLALHQVSGRSLVERLRAHPWIKDATVERLPPHTLAITVLERIPAAVLRTGSGNLL
                     CDAEGVVLSQLGVQDDPSLPLLIGFDAKALGQGEGQVRQAVQSGVNLAKLIAMTFDGR
                     VEIDVTNSSGLIASTKGIRFHFGNEALSAQWERFRKVRSSLRLAALDGKKRDMSEVDL
                     RYDNRVIVRERG"
     gene            1195..2430
                     /gene="ftsA"
                     /locus_tag="VI359_08575"
     CDS             1195..2430
                     /gene="ftsA"
                     /locus_tag="VI359_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY6974344.1"
                     /translation="MPKRDQILVGLDIGTTKIFAIVSEVTEDGTLNIIGVGSSPSRGL
                     RKGVVVDIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISAENCKGVVALKKSEV
                     TRDDIQRAVESARTLAVVPHERRILHVLPREFMVDGQEGVREPLGLSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIVLQPLASSEAVLGQEERDLGVAMVDLGGGTTDLA
                     IFLDGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKIQHGVARTELVQAHQTVEV
                     PSVGDRPARTFSRRDIAEILEPRVEEMFELVRREIARAGYEGMLGAGVVITGGTSLLE
                     GMPDAAEHVLNLPARRGVPTGVGGLRDIVSNPMHATGVGLLLHARRHMDELATAGLRN
                     GRPIAKVFDRMKSWMFEFF"
     gene            2480..3673
                     /gene="ftsZ"
                     /locus_tag="VI359_08580"
     CDS             2480..3673
                     /gene="ftsZ"
                     /locus_tag="VI359_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY6974345.1"
                     /translation="MMFSFQEDLASPVKIKVIGVGGAGCNAVNTMISAGLARVDFIAA
                     NTDVQALDRSRAAYKVQLGPERTRGLGAGAKPEVGKDSALESKDHIRECLEGSDMVFV
                     TAGMGGGTGTGAAPIVASIARELGILTVGVVTKPFQYEGHRRAAYAEEGIRDLRRHVD
                     TLLIIPNQRLLGIVDKATPLLEAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTV
                     MSHTGRAVMGMGVSRGTNRAIEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHE
                     IEEAASIIQQTADPEANIIVGQVINPDMGDDLVVTVIATGFERDDQPVTSAVDRLATA
                     RSPRTNQQVLAGVGATMTERSHKDLDRPAFLRRGSDPRENMDRITAVTDDEWDVPTFL
                     RKQAD"
     gene            3718..4449
                     /gene="pgeF"
                     /locus_tag="VI359_08585"
     CDS             3718..4449
                     /gene="pgeF"
                     /locus_tag="VI359_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY6974346.1"
                     /translation="MQAPVITVPAFASLRGGVQHFFGTRRHAGTLALDIGVPARGQDP
                     TQRGWMLSVKQVHGTEALVVDRPIRDSDRFAGEWDALVTNQPGVTVTVRTADCVPVLV
                     YDPRRRVVAAIHAGWRGAVADIVPKTLALMHARFGSSVADLRVSIGPSAGSCCYEVDG
                     PVLHQLRAGFEEWPSVVRGCDGDRARLDLKGLIRRQVERHGIDATQVSAVNLCTICHD
                     DLFYSYRREGRVSGTMVSGIALVSR"
     gene            4553..>4901
                     /locus_tag="VI359_08590"
     CDS             4553..>4901
                     /locus_tag="VI359_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974347.1"
                     /translation="MDAEATQSIGDNLRDVYKRIRRAALAAGRREDSIRLVAATKSVS
                     LDAIRQGLPAGLNILGESRLQEALPKIEALRGEPVRWHFIGRLQRRKARSVVGVFDLI
                     HSVDTLELAQEIDR"
BASE COUNT          990 a         1294 c         1622 g          995 t
ORIGIN      
        1 gccaaatatg aaaagggaaa aactcagtat ctctgcccgg ctcccttgtc ggcggcgatt
       61 accaaacaga ttcgcacgtt ggccttgaag acgtatcaag tcatgggatg cgacggggcc
      121 atcagggtcg attttcggat cacgccgcgc gggcgcccct atgttctgga aatcaacacg
      181 gtgccgggga tgaccgaaac cagtttgttg cctatggcgg cggctcaagc tggaattgac
      241 tacgacgagt tgacggagcg gattctccaa tcggcgttgg tgcgcgccag ggaacgtgtg
      301 gcttcgacga gagggtaacc cgatgatgtg gttgcgcaag agaccacgac gcgcagcggg
      361 gcctcggcag aatcagtgga agggaccgcg tgcgactcta tccgttcatc agcggcagca
      421 agcgcatcga gccagaagat gggcccgctg gcgtcgtgtc ctgcttgcaa gcggatggtt
      481 ggccgggggt gctgtggcgg tctggagcct catgttggcc gcaggggaga tcgccccgat
      541 tttacagcga gggttggaga ttcgcgaggt gaaggtgact ggggtccgcc atatgaccca
      601 acaggatgtg ctcgatcgac tcgcgttgaa aaaaggtctt gcgcttcatc aagtcagcgg
      661 gcgttccctc gttgaacggc ttcgcgccca tccctggatt aaggatgcga cggtcgagcg
      721 gctgccacct cacacgcttg ctattactgt gctcgaacgt ataccggcag cggttcttcg
      781 gactggatca gggaatctcc tctgcgatgc ggagggcgtt gtgttgagtc agttaggtgt
      841 ccaggacgac ccatcgctgc ccctgctcat cggcttcgat gcgaaggcgt tggggcaagg
      901 ggaaggacag gtaaggcagg cggtccagtc cggcgtgaat ctcgcgaagc tgattgcgat
      961 gacgttcgat gggcgggttg agatcgacgt gacaaattca tcgggcctga ttgcgtcaac
     1021 gaaagggatt cgctttcatt ttggcaatga agcactgagt gcccaatggg aacgcttccg
     1081 caaggtcaga tcttcgctca ggctcgcggc actcgacgga aagaaacgtg acatgagcga
     1141 agtggatctc cgatacgaca accgcgtgat cgtgcgcgaa agggggtgag ggctgtgcca
     1201 aagcgagacc aaattctcgt cggtcttgac atcggaacga ccaagatctt cgcgatcgtg
     1261 tccgaagtca cggaggacgg gaccctcaac atcatcggcg tgggatcgag tccgtcgcgt
     1321 ggtctgcgca agggcgtggt ggtggacatc gagagcaccg tcgaatcgat caagaaagcg
     1381 gtcgaagaag cggagctgat ggcggctgtc cagatcaact ccgtctatac cggcattgcc
     1441 ggcagccata tctcagcgga gaactgtaaa ggagtggtcg ctctcaagaa atctgaggtg
     1501 acacgtgatg atatccagcg tgcggtggag agcgcgcgga cgctcgccgt ggttccgcat
     1561 gagcggcgca tccttcacgt cctgccgcgc gagtttatgg tggacggaca ggagggcgtg
     1621 cgtgaaccgc tgggtctctc gggcaatcgt cttgaagtga acgtccatgt aatcaccgga
     1681 gccgtcacgt cggcgcaaaa tatcattaag agcgtcaatc gcgccgggct cgacgtcgtc
     1741 gacatcgtgc tgcagccctt ggcctcgagc gaggcggttc tgggtcagga agaacgcgat
     1801 ctcggtgtcg ccatggtcga tcttggcgga gggaccaccg atctggcgat tttcctcgat
     1861 ggcagcatcc gtcactccgc ggttctgccg atcggggggc agaatctcac gaaggatctg
     1921 gcgattgggt tgctgacctc tcaaacagaa gcggagaaga tcaagattca gcacggtgtc
     1981 gcgcgaacgg agcttgtgca ggcccaccag accgtcgagg tgccgtcggt cggcgaccgt
     2041 ccagcccgga cgttctcgcg ccgggatatc gccgaaattc tggagcctcg tgtcgaagaa
     2101 atgttcgaac tcgttcgaag agagatcgcc cgtgccggct acgaaggcat gttgggagcc
     2161 ggggtcgtca ttaccggcgg cacctccctc ttggaaggaa tgcccgatgc ggccgaacac
     2221 gtgttgaatt tgccggctcg gcgaggagtc ccgaccggtg tcggtggatt acgcgatatc
     2281 gtcagcaacc ccatgcatgc gaccggtgtg ggattgttgc tccacgcgcg gcgacatatg
     2341 gatgaactgg cgacggccgg tcttcgaaac ggacgaccga ttgccaaagt gttcgatcgg
     2401 atgaagtctt ggatgttcga gttcttttaa ccgtcgtgtg cggcatggcg ccgcgcgcga
     2461 gccacgtaag ggaggtgtca tgatgttctc atttcaggag gatctcgcat cccccgtgaa
     2521 aatcaaggtg attggagtcg gtggtgccgg gtgcaacgcg gtcaacacca tgatatccgc
     2581 ggggttggca cgagtcgatt tcatcgcggc caataccgat gtgcaggcct tggatcgatc
     2641 acgggcggcg tataaagttc agcttgggcc ggagcgcacg agaggactgg gcgctggtgc
     2701 caagccggaa gttggaaagg attctgcgct cgagagcaaa gatcatattc gggagtgttt
     2761 ggaaggctcc gatatggtgt tcgtcacggc gggaatgggt ggcgggaccg gcaccggagc
     2821 ggctcccatc gtggcaagca ttgcacgcga gttgggcatt ctgacggtcg gcgtcgtgac
     2881 gaagcccttt caatatgaag gacatcgccg tgcagcttac gcggaagagg ggatccgcga
     2941 tttgcggcgg catgtcgata ccttgcttat cattcccaat caacggttgc tcggcatcgt
     3001 cgacaaagcg acgccgttgc tcgaagcctt caaagtggca gacgacgtac tgcgacaggc
     3061 tattcaagga atcgcggatg tgattaccac cacgggccac gtgaacgtgg acttcgcgga
     3121 cgtgcggacg gtcatgtcgc acacgggccg cgcggtgatg gggatgggcg tttcgcgggg
     3181 gaccaaccgg gccatcgagg ccgcccagaa agccatctgt agcccgttgc tcgaggaagg
     3241 aagtgtggag ggggcccgtg gggtgttgtt gaacatcacc ggtggtccga acatgtcgct
     3301 gcatgagatt gaagaagccg cgtcgattat tcagcagacg gccgatcccg aagccaacat
     3361 tattgtcggt caggtgatca accccgacat gggtgacgat ctcgtcgtga ccgtgatcgc
     3421 gactggcttc gagcgggacg accaaccggt tacctcggct gtagatcgac tcgcgactgc
     3481 ccgtagcccg agaacaaacc aacaggtgct cgccggagtc ggggccacta tgaccgagcg
     3541 atcgcataaa gatctcgacc gtcccgcgtt tctgcgccgc gggagtgatc cgagagagaa
     3601 catggaccgt atcacggctg tgacggacga cgagtgggat gttcctacgt tcctgcgcaa
     3661 gcaagcggat tgatcacctc cgcagaacat cgtgaggtaa ggctaaggac atcggaaatg
     3721 caggcaccag tcatcacggt tccagcgttt gcctccttgc gaggcggtgt ccaacatttt
     3781 ttcgggacgc gtcgccatgc cgggacgctc gcccttgata tcggcgtgcc ggccagaggg
     3841 caggatccaa cgcagcgagg gtggatgctc tcagtgaagc aggtgcacgg aaccgaagcc
     3901 ctcgtggttg atcgaccgat ccgcgattct gatcggttcg ccggcgagtg ggatgccctg
     3961 gtgacgaatc agcctggagt cacggtgacg gttcggacgg ccgattgtgt gcccgttctc
     4021 gtctatgatc cgcgccggcg agtggtggcg gccatccatg cgggctggcg cggtgctgtt
     4081 gccgacattg tccccaagac attggcactc atgcatgcgc gctttgggtc atccgtggct
     4141 gatctgcggg tgagtatcgg tccgtcggcc ggctcctgct gctacgaggt cgatgggccg
     4201 gtcttgcacc agttgcgggc gggttttgag gagtggccca gtgtggtgcg tggatgtgat
     4261 ggcgataggg cacgtctcga cttgaaaggc ctcattcgcc gacaggttga acggcatggg
     4321 atcgatgcca cgcaggtgtc ggccgtcaat ctctgtacga tttgccacga cgacctgttc
     4381 tattcgtatc gacgagaagg gcgagtcagt gggaccatgg tcagtggaat tgcactcgtg
     4441 tcccgttagt catgagagca tgtcaccgct ctgccatttc cctggcactt tgggtacaat
     4501 attcgctgac gtgagcccag gtggaggcag gttagtcggt gtttaggacg ggatggatgc
     4561 agaggcgacc cagtcgattg gcgacaacct gcgggacgta tacaaacgga ttcgacgagc
     4621 agccctcgcg gccggacgtc gagaagacag tattcgactg gttgccgcaa caaagtccgt
     4681 ctcgctcgat gcgattcgac aaggtctccc ggctgggttg aatatcctcg gtgagtcccg
     4741 gctgcaagaa gcgcttccaa aaatcgaggc gttgcgagga gaaccggttc gttggcattt
     4801 catcggtcga cttcaacgac ggaaggcgcg gtccgtggtc ggtgtgttcg acctaattca
     4861 ttcagtcgat accctcgaat tggcacaaga aatcgaccga c
//
LOCUS       DATDNW010000358         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1350722, whole genome shotgun sequence.
ACCESSION   DATDNW010000358 DATDNW010000000
VERSION     DATDNW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350722"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VI359_08595"
     CDS             <1..1107
                     /locus_tag="VI359_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY6974348.1"
                     /translation="ERPQHRMYEQWHPLGPIGVITAFNFPVAVWAWNAFIAAIAGDTV
                     VWKPSPKAPLCAVAVQHICNQVMEQQGYRGIFSLLVTEEKTLAETMVHDRRLPLISFT
                     GSVPIGRQVASAVGQRLGRTLLELSGNNAIIVDESADLNLAIRAIVFGAVGTAGQRCT
                     TTRRLLVHESRYDEVVAKLASAYKQVRIGNPLEAGVLMGPLIDQAAVEGFRAAVEEAT
                     KEGGTILYGGHILNRPGFFVEPTLVQAQNHWSVVQRETFAPILYIMTFKTIEEAIAMH
                     NDVPQGLSSAIFTSDIRRSERFLSAAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGR
                     EAGSDAWKAYMRRQTNTINWGTDLPLAQGIQFGS"
     gene            1140..1733
                     /locus_tag="VI359_08600"
     CDS             1140..1733
                     /locus_tag="VI359_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY6974349.1"
                     /translation="MDQAAQLDTLISRMIDAYVSRNHAARVLRGLLEEAGVGFYPVAD
                     HLTLRTLDIDRRAEEYTRLGYAYSETLQYDDWYAKVYRKAGYPALFVDQAYPDDRGKT
                     SIIPGWVKKFGDQVYHHVAVRVDDIEKAIDRLKAKGVVFTGQIVGARGGPLRQIFTAP
                     EMIDGQPFSVLELAERHCGYQGFSPPQADSLMKSTIK"
     gene            complement(1735..2670)
                     /locus_tag="VI359_08605"
     CDS             complement(1735..2670)
                     /locus_tag="VI359_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEY6974350.1"
                     /translation="MTLTELRYIVAVAQERHFGRASARCFITQPALSLAIQKLEDELE
                     SAIFERRKNDVTLTPVGAKIVEQAQRVLEEAERIKLIAAQGKDQLVGPLRLGVIATVG
                     PYILPDLVPLLHKRAPAMPLEIEENLTANLTAMLKHGKLDAIIVALPFDEPGIVSQPL
                     YEEEFKLVVPVTHPWAHRKSVDSRALANEKVILPHAGHCFRLQVLEACPELSRSDMEG
                     MQGNSLETIRHMVASGLGITVLPSSALTPKYHNRRLTALSFAKPVPARQIGLAWRKGF
                     ARPDALKAVADSIRTLRIIGLRVLQPRASHIVGAV"
     gene            3020..>3270
                     /locus_tag="VI359_08610"
     CDS             3020..>3270
                     /locus_tag="VI359_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974351.1"
                     /translation="MKERRRMVVAFVCAFTVFGLLAGLPPGEGRGEQAAPGGKPTHAT
                     QGEYTLKIPFGLEETAVVIPADNPLTKEKVELGRLLFFD"
BASE COUNT          695 a          925 c         1007 g          643 t
ORIGIN      
        1 gaacgaccgc agcatcgtat gtatgagcaa tggcatccgt tggggccgat cggtgtgatt
       61 acggcgttca attttcctgt ggcggtctgg gcctggaatg cgttcattgc cgcgattgcc
      121 ggtgatacgg tggtctggaa accctccccg aaagcgccgc tctgtgccgt cgcggtccag
      181 cacatctgca accaggtgat ggaacagcag ggctaccgtg gcatcttttc gctcttggtc
      241 accgaagaga agacgctcgc cgagacgatg gtgcatgatc gccgcttgcc gctcatctcc
      301 tttaccggat ccgttccgat cggtcggcag gttgcctcgg ccgtggggca acggttaggc
      361 cgaacgttgc tggaactcag cggcaacaat gccatcatcg tggacgaaag tgccgacttg
      421 aacctggcca tccgcgccat cgtcttcggc gcggtgggaa cggcgggcca gcgttgcacg
      481 acgaccaggc ggctgttggt ccatgagtcc cgttatgacg aggtggtcgc gaagctcgcc
      541 tcggcctaca agcaggtgcg catcggcaat ccattggagg ccggtgtgct catggggccc
      601 ttgatcgacc aagccgccgt cgaagggttt cgtgccgccg tcgaggaagc cacgaaggaa
      661 ggaggcacca ttctctatgg agggcacatc ctgaaccggc ctgggttttt cgtcgagccc
      721 acgctcgtgc aggcccagaa ccactggtcc gttgtccagc gagaaacctt tgcgccgatc
      781 ctgtacatca tgacgttcaa gacgatcgag gaagcgatcg cgatgcacaa cgatgtgccg
      841 cagggcctct cctcggcgat ctttacgtcc gacattcgcc ggagcgagcg gtttctgtcg
      901 gcggcaggga gcgattgcgg gattgccaac gtgaacatcg gcacctccgg ggcggaaatc
      961 ggcggtgcat tcggcgggga aaaggaaacc ggcggcggac gggaagccgg ctccgatgcc
     1021 tggaaagcct acatgcgccg ccagacgaac acgatcaatt ggggcacgga cctgccgttg
     1081 gctcaaggca tccagttcgg gtcgtagcaa gattgtgtga gcaggaagga gtcgtgacca
     1141 tggaccaagc ggctcaactc gatacactga tcagccggat gatcgacgcc tatgtgtcgc
     1201 gcaatcacgc ggcccgcgtg ctccggggct tgctggagga ggccggtgtc ggcttctacc
     1261 cggtggcgga ccacctgact ctgcggacgc tggacatcga tcggcgcgcc gaagaataca
     1321 cgaggctcgg ctatgcctac agcgagacct tgcagtatga cgattggtac gccaaggtct
     1381 accggaaagc cgggtatccc gccctgttcg tggatcaagc ctatccggac gatcggggga
     1441 aaacgagcat tatccctggg tgggtcaaga agttcgggga ccaagtctac catcacgttg
     1501 ctgtgcgtgt ggacgatatc gagaaggcga tcgatcggct gaaggccaaa ggcgtcgtct
     1561 tcaccggcca gatcgtgggc gcgcgcggcg gcccattacg ccagatcttc accgctcctg
     1621 aaatgatcga tggccagccg ttttccgtgc tcgagctcgc cgagcgacat tgcggctatc
     1681 aaggattctc cccaccgcag gccgacagcc tgatgaaatc cacgatcaag tagcctacac
     1741 cgctcccact atatgggatg ctcggggctg taacaccctc aggccgataa tcctcaaggt
     1801 acgaatagag tccgccaccg ctttcaatgc atcaggacga gcgaatcctt tcctccaggc
     1861 cagcccgatt tgcctcgctg gtaccggttt cgcgaatgac agcgcggtga gtcgcctgtt
     1921 gtggtatttg ggcgtcagcg cgctcgaggg caacacggtg atgccgagcc cagaggccac
     1981 catgtggcgg atggtctcga gggagtttcc ttgcatccct tccatgtccg accggctgag
     2041 ttccggacag gcctctagga cttgaagtcg gaaacaatgt ccggcatgag gcagaatgac
     2101 tttctcgttg gccagcgcgc gggagtcgac ggatttcctg tgcgcccatg gatgagtgac
     2161 ggggacgacg agtttgaatt cttcctcgta gagtggctgc gaaacgatcc ccggctcatc
     2221 gaaagggagc gccacgatga tcgcgtccag tttcccatgt ttcagcatcg ccgtgagatt
     2281 cgccgtgagg ttctcttcga tttccaatgg catggccggt gcccgcttgt gcaaaagagg
     2341 aaccaagtcc ggcaggatat acggtcctac ggtggcgatg actcccaacc gcagagggcc
     2401 gacaagttga tccttcccct gtgccgcaat cagcttgatg cgctccgctt cttccagcac
     2461 tcgttgcgcc tgctccacga tctttgctcc aaccggcgtc agcgtcacgt cgttcttgcg
     2521 gcgctcgaag atcgcgctct ccaattcatc ctccaacttc tgaatggcca aacttaaggc
     2581 cggctgtgta atgaagcagc gagccgacgc acgaccgaag tgtcgctcct gtgcgacggc
     2641 cacgatatag cgaagttcgg tcagggtcat gagtcaggtt cgcacgaaat aacaccggac
     2701 gaattagtaa aacatattga tacacctagc acaatcaatt ggactaatca acgaacagac
     2761 cttacagtga atgcaggatg atcaactcgc accagagtcg tgtaaggaag gagactccat
     2821 gaggcacgca tgattcacag gaagaagtat ggtgatcgcc gcagtcctga tggccggctg
     2881 cgcagccaaa cgacatggac aggtgaacga ggctggagaa cgacccagta atctgagacc
     2941 agccgatata gccgttgatt cagagatgca ccgagggcag aatggcaggg cttgcactgc
     3001 ataaagggag gagatggaaa tgaaggaacg gagaaggatg gttgtggcgt tcgtatgtgc
     3061 gttcacggtg ttcgggctgc tcgcgggact ccctcccggt gagggacgag gggagcaagc
     3121 agcgcctggc ggcaagccga ctcatgcgac acagggagaa tacacactca agattccgtt
     3181 cggcttagaa gaaactgcgg tggtgattcc tgccgataac cccctgacga aggagaaggt
     3241 tgagctcgga cgcctcctgt tcttcgacaa
//
LOCUS       DATDNW010000359         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_394757, whole genome shotgun sequence.
ACCESSION   DATDNW010000359 DATDNW010000000
VERSION     DATDNW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394757"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..2475
                     /locus_tag="VI359_08615"
     CDS             67..2475
                     /locus_tag="VI359_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY6974352.1"
                     /translation="MGNSLSQKELKLLDAYWRAANYLSVGQIYLYDNPLLKKPLKKEH
                     VKPRLLGHWGTTPGLNFIYAHLNRVIKQRDLNMIYIIGPGHGGPGIVANAYMEGTYSE
                     VYPNIAQDEEGMKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAYDNP
                     NLIVACVVGDGEAETGPLATSWHSNKFLNPLSDGVVLPILHLNGYKIANPCVLARISH
                     QELDQLFRGYGYTPHFVEGSDPEKMHQLMASTLDNILEDIHRIKADASRTGGKKRPLW
                     PMIILRSPKGWTCPKEIDGKRCEDYWRSHQVPMGEMHEKPEHVKILEKWMKSYKPEEL
                     FDKTGRLKPELAELPPKGHRRMSANPHANGGLLLRDLRLPDFRDYAVKVTKPGAITAE
                     STRLMGKFLRDTMKLNMESQNFRLFSPDENNSNRWQDAMEVTNRAWMADRYPYDDHLA
                     PDGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLKVCHDIP
                     WRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKADVVRVYLPPDANTLLSVT
                     DHCLRSRNYVNVIVAGKQPAPQWLTMDQAVKHCTAGIGIWEWASNDKGSEPDVVMACC
                     GDVPTLETLAAVDLFRQHLPALKIRVINVVNLMKLQPQSEHPHGLSDQDFDALFTKDK
                     PIVFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTSTPFDMVVMNDLDRFHLVGDV
                     IDRLPQLGSRAAYAKQAIGDKLLEHKQYIAKYGEDMPEITDWQWGQRKTETKRRTSTE
                     GDNV"
     gene            2700..>3065
                     /locus_tag="VI359_08620"
     CDS             2700..>3065
                     /locus_tag="VI359_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974353.1"
                     /translation="MKQEDLKDILQQYGVGPIQFSGTNDALYERHLTFDHVVDVKKAD
                     LREQFEAAAHAIRDIISQRWLRTEDTYAKKNPKRVYYLSMEFLLGRSFTNNIVNASLY
                     PLAMEAAKKKGLDPLALYEM"
BASE COUNT          744 a          849 c          875 g          597 t
ORIGIN      
        1 acacattcat aaaccaacag cgctggacat tggttgagcg cgggctgtaa cagagaagga
       61 gaggtcatgg gaaattcgtt gtcgcaaaag gaacttaaat tgctggacgc ctattggcgt
      121 gcggcgaact atctgtcggt cgggcagatc tatctgtacg acaatcccct gctgaaaaag
      181 ccgttgaaga aagagcacgt caaaccccgt ctgctcggtc actggggaac caccccggga
      241 ctgaatttca tctatgcgca tttgaatcga gtgatcaagc aacgggatct caacatgatc
      301 tacattatcg gtccaggcca cggcggtccc ggcatcgtcg ccaatgccta catggaagga
      361 acctactccg aggtctaccc caacatcgca caggatgaag agggaatgaa gcggctgttc
      421 actcagttct catttccagg tggcatcccc agccacgtgg cacccgagac tccgggctcg
      481 atccacgaag gaggcgaatt ggggtacgcc gtttcgcacg cgtacggcgc cgcctacgat
      541 aatcccaacc tgatcgttgc ctgtgtggtt ggcgacggtg aggcggaaac gggcccgctg
      601 gcgacctcct ggcactccaa taagtttctt aaccccctct ccgatggtgt ggtgcttccg
      661 atcctgcacc tgaacgggta caagattgcc aatccctgtg tgctggcccg tatcagtcat
      721 caagaattgg atcagctctt ccgcggctac ggctacacac cccactttgt cgaggggagc
      781 gacccggaaa agatgcacca actaatggct tccacgctgg ataacatcct cgaagatatt
      841 caccgcatca aggctgacgc tagccgtacc ggcggcaaga agcgaccgct ctggccgatg
      901 atcattctcc gttcacccaa gggatggacc tgtccgaagg agattgacgg gaaacggtgc
      961 gaggattatt ggcggagcca ccaggttccg atgggtgaaa tgcatgaaaa acccgagcac
     1021 gtgaagatcc tcgaaaaatg gatgaagagt tataagccag aggaactgtt cgacaagacc
     1081 ggccggttga aacccgaact cgcggaattg ccaccaaaag gccaccgtcg catgagcgcc
     1141 aatccacacg ccaacggcgg tttgttgctc agagacctgc ggcttccgga ctttcgtgac
     1201 tatgccgtca aggtaacaaa gcccggcgct attacggccg agtccacccg cctgatgggg
     1261 aaattcctgc gggacacgat gaagctgaac atggagagtc agaacttccg cctgttcagt
     1321 ccggacgaaa acaactccaa tcgctggcag gatgcaatgg aagtcacgaa tcgcgcctgg
     1381 atggcggatc gctacccgta cgacgaccat ttggccccgg acggcagagt gatggaaatg
     1441 ctcagcgagc accaatgcca aggctggctg gaaggatatc tgttaaccgg ccggcacggg
     1501 ttcttctcct gctatgaggc gttcattcac atcatcgact cgatgttcaa tcagcatgcc
     1561 aaatggctca aggtctgcca cgacataccc tggcggcggc cgattgcctc gctcaattat
     1621 ctcttgtcgt cccacgtctg gcggcaggat cacaacggat tcagccatca ggatcctggg
     1681 ttcattgatc acgtcgtcaa caagaaggcc gatgtcgttc gtgtgtatct tccgccggac
     1741 gcgaatacgc tgctctcagt gaccgatcac tgcctgcgca gccgtaatta tgtgaacgtc
     1801 atcgtcgcag gcaagcagcc ggccccgcaa tggttgacga tggatcaggc ggtcaagcat
     1861 tgcacggccg gcattggaat ttgggaatgg gccagcaatg acaagggaag cgagcctgat
     1921 gtggtgatgg cgtgctgcgg ggacgtgccg acgctggaga cgctggccgc cgtggatctg
     1981 tttcggcagc acctcccggc gttgaagatc cgggtcatca acgtcgtcaa cttgatgaaa
     2041 ctgcaaccgc agagcgagca cccgcatggc ctgtcggacc aggacttcga tgcgctcttc
     2101 accaaggaca agccgatcgt gttcgccttt cacggctatc cgtggttgat ccatcggctc
     2161 acgtaccggc ggacaaacca caagaacctg cacgtgcgcg gttacaagga ggaaggcacg
     2221 acgagcacgc cgttcgatat ggtggtgatg aatgatctcg accggtttca cctcgtcggc
     2281 gacgtgatcg atcggctgcc gcaactcggg tcccgcgccg cctatgccaa gcaagcgata
     2341 ggggacaagc tgcttgagca caagcaatac atcgccaagt acggagagga catgcccgag
     2401 atcaccgact ggcaatgggg gcaaagaaag acggaaacga agcggaggac ctcgaccgaa
     2461 ggcgacaacg tctaagcggc gactgacata cgttctgttg tttggttcgg agagggctgg
     2521 cagaagtacg tgagggtgta tcagaatcgt tcttcttatc tcaggatgcg atgcggaagc
     2581 agccatcggg gcaccgcgtg tcgtcctgag agagaagata ttgtccacat gatgagagtg
     2641 ggataaggaa gccaggtcgg cgaagacacc gcttccttga accgcatcgc ggaggctcta
     2701 tgaaacaaga ggacttgaag gacattcttc aacaatacgg agttgggccc atccaatttt
     2761 ccggcacgaa cgacgcgctc tacgagcggc atctgacgtt cgaccacgtt gtcgacgtca
     2821 agaaagccga tttgcgtgaa cagtttgaag cggcggcaca tgcgatacgc gacatcattt
     2881 cacagcgctg gctccggacg gaagacacct atgccaagaa gaatcccaag cgggtctatt
     2941 acctctcgat ggaatttctc ctcgggaggt ccttcaccaa taacatcgtg aatgcctcgc
     3001 tctacccatt ggcaatggag gcagcaaaaa agaaaggcct cgacccgctg gcactgtacg
     3061 aaatg
//
LOCUS       DATDNW010000360         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_474907, whole genome shotgun sequence.
ACCESSION   DATDNW010000360 DATDNW010000000
VERSION     DATDNW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474907"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VI359_08625"
     CDS             <1..1208
                     /locus_tag="VI359_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY6974354.1"
                     /translation="TGFAAVSLQPNAGSQGEYTGLMVIRAYHRSQGEFHRDVCLIPVS
                     AHGTNPASAAMVGLTVVPVACDRQGNVDLADLEAKAIQYRDRLAALMLTYPSTHGVFE
                     ANVRRICQIVHTHGGQVYMDGANMNAQVGLCRPGDIGADVCHLNLHKTFCIPHGGGGP
                     GMGPIGVARHLSPFLPGHPVVGLGGEQSIGPVSAAPYGSPSIMTISWVYMALMGRDGM
                     TMATQIAILNANYMAKRLEKYYPILYTGTSGFVAHEFILDLRQFKESAGVEAMDVAKR
                     LMDYGFHAPTVSFPVAGTLMIEPTESESRAELDRFCDAMILIRGEIQDIVDGRQPRTN
                     NLLKNAPHTALTVTANEWDRPYSREQAAFPAPWVRDHKFWPSVGRIDEAYGDRNLVCT
                     CPPMDTYST"
     gene            1337..>2567
                     /locus_tag="VI359_08630"
     CDS             1337..>2567
                     /locus_tag="VI359_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY6974355.1"
                     /translation="MSAIDRRDFLASTGLALGAALLTAELPFLKTGAAAASVVNLDSW
                     ESVRGQFSLSPGLVHMAGFFLASHPASVREAIDRHRRGLDADPIGYWFEQEEKQETTV
                     LRTIADYLAADPTEIALTDSTTMGLGLLYGGLQLRSNQEILTTTHDHYSTETSLRLRA
                     ERTGATVRQIPLYKSLKTVSQSELVDTLVQQVRPQTRVIAVTWVHSSTGLKLPIHDMA
                     TAIATINRTRSEQDRVILCVDGVHALGVEDFRLSEVGCDFLVAGTHKWMFGPRGTGLV
                     WGHKRAWPVAHPTIPTFNTQAYDIWMQLTPPKELPPSVYMTPGGFHSFEHRWALDEAF
                     KFHQAIGKSRVTQRIYELNQQLKQGLAKMSHVTLHTPLSQDLSAGIVCFDVVGLPARH
                     VVERLRERGIIGSVTPYA"
BASE COUNT          523 a          720 c          747 g          577 t
ORIGIN      
        1 ttacggggtt cgctgcggtc tcgttgcaac cgaacgctgg ttctcagggc gaatatacgg
       61 gactgatggt gatccgagcc tatcatcgca gtcagggcga gtttcatcgc gacgtctgtt
      121 taattcctgt ttctgcgcac ggcaccaatc cagccagtgc ggcgatggtg ggattgaccg
      181 tcgtgccggt cgcttgcgat cggcagggca acgtggatct cgcggatctc gaagcaaagg
      241 ccattcagta tcgtgatcgt ctcgcggctc tgatgctgac ttacccgtct acccacgggg
      301 tatttgaagc gaacgttagg cgtatctgtc aaatcgtcca tacacatggc ggacaagtct
      361 acatggacgg agccaatatg aacgcgcagg tgggactctg ccggcccggc gatatcggtg
      421 cggatgtgtg ccacctgaat ctgcacaaaa cattttgcat tccccacggc ggcggcggac
      481 cgggtatggg tcccatcggt gtggctcgac acctgtcccc gtttctcccg ggtcatccgg
      541 tcgtgggact cggtggggaa cagtcgattg ggccggtgtc ggcggctccc tatggcagcc
      601 ccagtatcat gacgatctcg tgggtatata tggcgctgat gggtcgggac ggaatgacca
      661 tggcgaccca gattgccatt ctcaacgcca actatatggc caagcgactt gagaaatact
      721 atccgatcct ctatacgggt acgtcgggat tcgtcgcgca cgagtttatc ctcgacttac
      781 ggcaattcaa ggagagtgcc ggcgttgaag cgatggatgt cgccaagcgc ctgatggact
      841 atggcttcca tgcgccaacc gtgtcattcc cggtcgccgg gacactgatg atcgagccga
      901 cggagagcga atcaagggct gagctggatc gattttgcga tgcgatgatc ttgattcgcg
      961 gcgagatcca agacatcgtc gatggtcgcc agcctcgaac gaacaatctc ctcaagaatg
     1021 cccctcatac tgccttgacg gtgacggcga atgaatggga tcgaccgtac agtcgtgaac
     1081 aagcagcctt tcctgcgccc tgggttcgag atcacaagtt ttggcccagc gtgggccgta
     1141 tcgacgaagc ctacggggat cgcaaccttg tctgcacgtg cccgccgatg gatacctatt
     1201 ccacctagga agtatccaac cgcctctttt cgctgtcgtc attagcttcc agagaccccc
     1261 gcttgccgct tcccgacaag cgccgtagag taagcgaagc ttgctggaat cgtcgttctc
     1321 tgcaaggagg tttctcatga gcgcgatcga tcgcagggac tttttagcta gtactgggtt
     1381 ggcattggga gcggcgctgc tcaccgccga actgccattt ctgaagaccg gcgctgcggc
     1441 agcgtcagtg gtgaatcttg acagctggga atctgtacgt ggtcagtttt ctctttcgcc
     1501 cggtctcgtg cacatggccg ggttttttct cgcctcgcat cctgcatcgg tgcgtgaagc
     1561 catcgaccgg catcgccgcg gtctcgatgc cgatcccatc gggtactggt ttgaacaaga
     1621 agagaaacag gaaacgacgg tcttgcgcac catcgccgat tatcttgcgg ccgatcccac
     1681 tgaaatcgcg ttgactgaca gtacgacgat gggcttaggg cttctatacg gtggccttca
     1741 gcttcggagt aatcaggaaa ttctgaccac gactcacgat cactattcga cggaaacgtc
     1801 cttgcgcctc cgagccgaac ggactggtgc gacagtccgt cagattccgc tgtacaaatc
     1861 tttgaagacg gtgtcccaaa gcgaactggt cgataccttg gtgcagcagg tgcgtcctca
     1921 aacgcgggtc atcgcagtaa cctgggtcca ttcgagcacc ggactcaagc tgccgatcca
     1981 cgacatggcg acggccattg cgacgatcaa cagaactcgc tcggaacagg atcgggtgat
     2041 cctgtgtgtc gatggcgttc acgctctggg cgtcgaggat tttcgcttaa gtgaagtcgg
     2101 gtgcgatttc ctggtcgcgg gaacacacaa atggatgttc ggcccgcgtg gtaccggtct
     2161 ggtctggggg cacaagcgtg cctggccggt tgcccatccg acgattccca ctttcaatac
     2221 gcaggcgtac gatatatgga tgcaactcac acctccaaag gagttgcctc cgtccgtgta
     2281 tatgaccccc ggagggtttc attccttcga gcatcggtgg gcgctcgatg aagcgttcaa
     2341 gtttcatcaa gccatcggca agagccgcgt cacacagcgg atttacgaac tgaaccaaca
     2401 actgaaacag ggactggcca agatgtcgca tgtgacgctg catactcctc tctcgcaaga
     2461 tctttcagcc gggatcgtct gtttcgacgt ggtcggactc cccgcacgtc acgtggtcga
     2521 gcgactgagg gaacgaggca ttattgggag cgtgactccc tatgcca
//
LOCUS       DATDNW010000361         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_262820, whole genome shotgun sequence.
ACCESSION   DATDNW010000361 DATDNW010000000
VERSION     DATDNW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262820"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VI359_08635"
     CDS             complement(<1..677)
                     /locus_tag="VI359_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974356.1"
                     /translation="MSVPVVLSPAWRDQIVDALQYHPSSRVLIHAPWAVRLASLQQMV
                     SFACGRKKTSLIIVGEAEKAEWLADSLCRDSERTVVCFHSGLPSDVRARIWEQISQGT
                     VGVVVGTRSAVFLPVRDLGLVWVDEEEDSALKEPQEPRYHARDVAWTRVQEEQAVLVL
                     SSSHPSLETIERVKHGGRLLHQSPPLESRPTVQVVDLRQQEKGASLSQPLVQAMRAAL
                     HQQSGAV"
     gene            complement(1262..>2596)
                     /locus_tag="VI359_08640"
     CDS             complement(1262..>2596)
                     /locus_tag="VI359_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY6974357.1"
                     /translation="GDRMIVGQRDTVYLRLKNPDDVAVGDFFTIYKRARKVFHPGTGQ
                     YLGYLIHRLAIVQVVQADHQLTTVKTIRSYGAVQPGNPVMKFSLPTAGEMAPDQPAES
                     EVQGMIVEYQADREMTLLAQRNVVYIDKGWEDGVKAGDRMEVVRVGGNLPPRLVGELK
                     ILSTEARTASALVSKSPSRILKGDRVRTKVHAPEAMPVSQPVQPSASEPTAASKQELA
                     SRKFPVQNATGETRINLNDLAKELRFESGEATIKPEGYRVLDQVVEYLKTEAGDKLIR
                     VEGHADNMEIGPSLKSRYPTNWDLSKARATGVARYILEKSGLDSARLSSVGFGDTRPM
                     VSNATETGRQQNRRVDVVLYAPEAAQQSPESVKEPAETMDSGYSFSSLGSQEGASQAV
                     PEEKQAPAVSSAPTEGKAPSPPVTDSTPTPADQPGTNQNPGTPNNSPVPPAQ"
BASE COUNT          560 a          742 c          722 g          572 t
ORIGIN      
        1 aatactgccc cagattgttg atgcagagcc gcgcgcatgg cttgcaccag tggctgactc
       61 aagcttgctc ccttttcctg ctgacggaga tcgaccactt gaaccgtagg ccttgattcg
      121 aggggtggag actgatgaag caggcggccg ccatgcttga cccgttcgat agtctcgagt
      181 gaaggatggg aagagctgag aacgagcacc gcctgttctt cttgcaccct agtccatgcg
      241 acatcacggg catgatagcg aggctcctgt ggttccttca gcgcggaatc ttcttcttca
      301 tccacccata ccaagcccaa gtcccgcact ggaagaaaga cggcggatcg tgtaccgacg
      361 acgaccccga cggtaccttg gcttatctgc tcccagatgc gggccctcac atcggatggc
      421 aatccgctgt gaaagcagac gactgttcgt tcggaatcgc ggcacagtga atcggcaagc
      481 cattcggctt tttcagcttc tccgacgata atcagactag tcttctttct cccacaagca
      541 aaggacacca tctgttgtaa cgacgccagc cgaactgccc acggagcatg gatgaggacc
      601 cgcgatgatg gatggtattg gagagcgtca acgatctgat cccgccacgc tggcgagaga
      661 acgacaggaa ccgacatcga agaatgttca tcgagaggaa gaagtgactc aacttgtgta
      721 ccccttggta atctcgcctt cttgggtcga accgcagtct tcgacagaac cagcggtggg
      781 agcacgagtc tcaagcactg tccccacggc gtgacatact ctttggcaat gcgtcgtgaa
      841 agatcgagca gtgatagcgg aacttccgct gagtgatctc cttggaacag tccgcgaatt
      901 tccttgagtc tggtgcgttc gagacctggt ggcagagagt gcatcaaggc agtgacggcc
      961 cctgccaaaa tcgatgatcc aaaagggacc agcactcggt gtccaatccg cagcgagctt
     1021 cgaaggtgcg caggaatacg gtaggtgaaa ggaccggtca gatgccgagg gacaatgacg
     1081 tcggcgtaac agacttcagg agatagttca cgaactgccg aatgaggact ctccatgcgg
     1141 cattgtagca ataggagaaa agagggaaca atgtcggaat aacagagtta aagcgagccg
     1201 cgtgtccggc gcgaaagggg tggcgaaaaa ccgccacccc tcgccgcgaa ggcccactgt
     1261 cttactgagc aggaggaact ggagaattgt ttggggttcc agggttctga ttcgtccctg
     1321 gctggtcagc gggcgtcgga gtggaatccg tgacgggagg tgaaggagct ttcccttcgg
     1381 ttggagccga tgagacagcc ggggcctgtt tctcctccgg aactgcctga ctcgcgcctt
     1441 cttgtgaacc cagacttgaa aaactgtatc cactgtccat ggtctccgct ggttccttca
     1501 ccgactcagg tgactgctgg gccgcctccg gtgcatacag cacgacgtcg acgcgtcgat
     1561 tctgttgacg tccagtttct gtcgcattgc tgaccatcgg ccgcgtatcc ccgaatccaa
     1621 ccgaggacaa ccgtgccgag tcaaggccgc tcttctcgag tatgtagcgg gccactccgg
     1681 tggctcgtgc cttcgacagg tcccaattgg tcggataacg tgatttgagc gaaggaccaa
     1741 tctccatgtt gtccgcatga ccttctaccc gaatcagctt atctcctgcc tcggtcttca
     1801 ggtactccac aacctgatcc agaactcgat atccctccgg cttgatcgtg gcctcacctg
     1861 attcgaatcg gagctctttt gccagatcat tgaggttgat tctggtttct ccagtcgcgt
     1921 tctgcacagg aaacttgcgc gacgcaagtt cctgttttga tgcagcggtg ggctcgctcg
     1981 cgctcggctg caccggttgc gagactggca tcgcctcagg agcatgaacc tttgtacgaa
     2041 cccggtctcc cttgaggatt cgagagggcg acttcgaaac cagcgcagac gccgttcgag
     2101 cctccgtcga caggatcttg agttccccca caagccgtgg aggaagattt cccccgacgc
     2161 ggacgacttc catccggtca ccagctttca caccgtcttc ccagccttta tcgatataca
     2221 cgacgtttcg ctgtgccagc agcgtcattt cccgatcggc ttggtattcc acaatcattc
     2281 cttggacctc gctctccgcc ggctgatcag gcgccatttc cccagcggtc ggaagcgaaa
     2341 acttcataac cgggttcccg ggttgcactg ctccgtagga ccgaatggtc ttgaccgtcg
     2401 tcagctgatg gtcagcttgg acgacctgaa ctattgcgag acggtgaatg aggtagccaa
     2461 gatactgacc agtgccggga tggaacactt ttcgagcgcg tttatagatc gtgaagaaat
     2521 ctccgaccgc gacatcgtcc ggatttttta agcggaggta gaccgtgtcc cgttgtccca
     2581 caatcatccg atcgcc
//
LOCUS       DATDNW010000362         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1154753, whole genome shotgun sequence.
ACCESSION   DATDNW010000362 DATDNW010000000
VERSION     DATDNW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154753"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VI359_08645"
     CDS             complement(<1..146)
                     /locus_tag="VI359_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY6974358.1"
                     /translation="MEFTEEQINRYSRHILLPEVGGKGQKKLAKAKILIVGAGGLGSP
                     AALYL"
     gene            complement(231..1160)
                     /gene="cysK"
                     /locus_tag="VI359_08650"
     CDS             complement(231..1160)
                     /gene="cysK"
                     /locus_tag="VI359_08650"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY6974359.1"
                     /translation="MTMVLHKEITELIGKTPLVRLNRLSPPDSATIYGKVEFFNPGGS
                     VKDRICLNMINEAERQGRLKPGGTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTPAWEGMKGSIREAESIIAQNPSYFMPDQFSNPANPAMHRMTT
                     APEIWEALGGKIDAFVAAVGTGGTITGCGEVFKEKNPHVKVIAVEPAASPVLSGGDPG
                     PHKIQGIGAGFVPKVLNQRILDRVITVTDDEAYQTAKALSKKEGLLVGISAGANVFAA
                     QKVAQELGPGKNVVTVLCDTGERYISIEKYFNI"
     gene            complement(1157..1357)
                     /gene="thiS"
                     /locus_tag="VI359_08655"
     CDS             complement(1157..1357)
                     /gene="thiS"
                     /locus_tag="VI359_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY6974360.1"
                     /translation="MQVKINGKMENIQGGTVLDLLKAKNIEPQMVAIEVNDAMLERNH
                     LATTTLKEGDQIEFLFYMGGGR"
     gene            1506..1883
                     /locus_tag="VI359_08660"
     CDS             1506..1883
                     /locus_tag="VI359_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974361.1"
                     /translation="MWKQNFMFRASEATPLKESEQELFHETDPAMDSAGLQLEKFLSV
                     WIQGEGNDDKPSTYSSLYVRTATLDFQKRVGFLQPLQGRSHQIKALLTPGQKQFLRDW
                     LSRQAPQAWEASDDHFRDLFEVE"
     gene            1880..2452
                     /locus_tag="VI359_08665"
     CDS             1880..2452
                     /locus_tag="VI359_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY6974362.1"
                     /translation="MTASVNLSRFGSPMLFLWLNLLVIAPATLPVSSSATTIEVWYAP
                     EDAPLDRVVALYNRAHRYLYVSVYGLSNPRAVEALVAAKKRGIDVRVLTDRERMDDPK
                     QRAAVNTLRLAGIPIKVNQHDGLMHLKQVVMDDEVNASGSMNHTTSGNRYNDERLDVI
                     TDKVITAKARDKFLAMWNDQERYQPWSEGR"
BASE COUNT          517 a          686 c          673 g          582 t
ORIGIN      
        1 agatacagag cggcggggga gccgagtccg ccggcgccga cgatcaaaat cttcgccttc
       61 gccagcttct tctgcccctt gcccccgact tctggcagca ggatgtggcg actgtatcta
      121 ttgatttgct cttcagtaaa ttccatgact ttgtccaggc tagtggttag aggcacgggg
      181 ccagaggtat ttcgagactg gttgcttctt tgccgcctct cgcctcgcac ctaaatatta
      241 aaatacttct cgatgctaat ataccgctcg ccggtatcgc acaacacggt cactacattc
      301 ttgcccggac cgagttcctg cgcaactttt tgggctgcaa acacgttggc gccggctgaa
      361 ataccgacga ggaggccttc cttcttcgag agggccttgg ccgtttgata ggcttcgtcg
      421 tcggtgaccg tgatgacgcg gtcgaggatc ctctggttca aaaccttggg gacgaatcca
      481 gcgccaatcc cttgaatctt gtgggggccc gggtctccgc cggaaagcac gggtgaggca
      541 gccggctcga cggcgataac cttgacgtgc ggattctttt ccttgaacac ttcgccgcat
      601 ccggtaatgg ttccgcccgt gccgaccgcc gcgacaaacg catcgatctt gccgccgagg
      661 gcttcccaaa tttccggcgc cgtcgtcatc ctatgcatag ccggattcgc cggattcgaa
      721 aattgatccg gcatgaaata ggatggattc tgggcgataa tgctttctgc ttctcgaatt
      781 gaacccttca tcccctccca cgccggtgtc agcacgagtt gcgcgccgta agaggagaga
      841 aggcttgccc gttccatact catgctctcc ggcatgacta aaatcaactt atagccgcgt
      901 accgctgcca ccagggcgag gccgatcccc gtattgccgc tcgtcggttc gacgatcgta
      961 ccgcccggct tcagtctgcc ttggcgttcc gcctcattga tcatattaag gcagatccgg
     1021 tcttttacgc tgccacctgg attgaagaat tcgaccttgc catagatcgt cgcggaatca
     1081 ggaggcgaca agcggttcag tctgacgagc ggggtcttcc cgatcagctc ggtaatttct
     1141 ttatgaagga ccatcgtcat cggccgccac ccatgtagaa caagaattca atctgatcac
     1201 cctctttgag ggtggtggta gcgagatgat tgcgctccaa catcgcgtcg ttcacctcga
     1261 tggcgaccat ctgcggttca atgtttttag ccttgagaag gtcgaggacg gtaccaccct
     1321 ggatgttctc cattttcccg ttgattttga cctgcatgcc actcctaaat tggcctagag
     1381 attaaggaat ttcaagaggt taacaaagac tcttcgatat tgtcaaggta accggcccag
     1441 tgtgcaagtt gcttgacttt ggtcttggtc cacggccact atgccgctcc ttggagacgg
     1501 catcgatgtg gaaacagaat ttcatgttcc gagccagcga ggcgacacct ctcaaggagt
     1561 ccgaacagga actctttcac gagaccgacc cggccatgga cagcgccgga ttgcagttag
     1621 agaagttttt gtccgtatgg attcaggggg aggggaacga cgacaagccg tcaacctatt
     1681 cgagcctcta tgtccgcacc gccacgttgg attttcaaaa acgggtcgga tttctccagc
     1741 ctctccaggg tcggtctcat cagatcaaag ctctccttac gccggggcag aagcaatttc
     1801 tgcgtgactg gctaagtcgc caggcgccgc aagcctggga agcatccgat gatcactttc
     1861 gggacctgtt tgaagtcgaa tgaccgctag tgtcaatctc tccagattcg gctctcccat
     1921 gctctttctg tggctcaatc tgcttgtcat tgccccggcg acccttccgg tttcgtcctc
     1981 agcgacgacg atcgaagtct ggtatgcgcc tgaggatgcg ccgctggatc gcgtcgtcgc
     2041 cctgtacaat cgggcacatc gatatctcta tgtttcggtc tatggcttga gtaatccacg
     2101 agcggtggaa gcactcgtgg cggcgaaaaa acgcggaatc gacgtgcggg ttctcaccga
     2161 ccgagagcga atggacgatc cgaaacagcg ggcagcggtg aacacacttc gattggccgg
     2221 catccccatc aaggtgaatc aacatgatgg gctgatgcac ctgaagcagg tggtgatgga
     2281 tgatgaggtc aatgcgtccg gctccatgaa ccatacgacg agcggcaatc gctataatga
     2341 cgaacggctt gatgtgatta ccgataaggt catcacggcc aaggctcgcg ataagtttct
     2401 cgccatgtgg aatgatcagg aacggtatca gccttggtcg gaaggacggt aggggtgg
//
LOCUS       DATDNW010000363         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_120583, whole genome shotgun sequence.
ACCESSION   DATDNW010000363 DATDNW010000000
VERSION     DATDNW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120583"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VI359_08670"
     CDS             <1..220
                     /locus_tag="VI359_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY6974363.1"
                     /translation="LEGKITAQWASLLDGECRTILRQKKTVHLDCAKVDFMDEQGMEV
                     LKNLPRKNVTLIGAPEYVTELLHIGGRS"
     gene            217..936
                     /locus_tag="VI359_08675"
     CDS             217..936
                     /locus_tag="VI359_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY6974364.1"
                     /translation="MNSVATTYTASLGHAPSVNRKTQSAERPGLTRSEDRLIARLRHG
                     DEGAFDELVNQHHGSLIRVALGYVADRDVAEEVVQDTWMTVIEKLDSFEGRSSLRTWI
                     FGILIHKAKDRGVREKRHTTFSALESYDDDNEEAVDPARFQQSGEWAGHWAFPPQPWD
                     DRTPETLLASKQATDCMQQAIQDLPSTLRDVLIMRDVEGIESTEICELLKITETNLYV
                     RLHRARERVRQAVETYLEGGQ"
     gene            1001..1273
                     /locus_tag="VI359_08680"
     CDS             1001..1273
                     /locus_tag="VI359_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY6974365.1"
                     /translation="MKDHPSQWIVSGLTCREVTDRTSDYLDERLPIVTKIRVALHLAS
                     CADCRAYVQQLALVRDAAALLPKPHLSPINQLRLRQHFARYHALLQ"
     gene            1377..>2590
                     /locus_tag="VI359_08685"
     CDS             1377..>2590
                     /locus_tag="VI359_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY6974366.1"
                     /translation="MESALFYDRLQFAITATFHYLFPQLTMGLALLLFYLRSRALRTG
                     DEHYHRVARFWTTIFALSFAFGVVTGIPLEFQFGTNWAKFSNFAGGVIGQTLAMEGMF
                     AFFLESSFLGILLYGENRFSRRTQWFASLMLFLGSWLSGYFILATNAWMQHPVAYTIA
                     PDGRLFVDSLSGLLTNPWLFWQFTHNMTAAVVTASFVMAAVGALYLLSNTHLDYGRTF
                     VRTGVVAGAVAAALMIFPTGHGNAKQVFEHQPVKGAAFEGLFRTEHGADILLVGQPNM
                     ETMTIDNPIVVPDALSILVYNRLYAKVKGLDAFPRKDWPDNLPLLYYSYHVMAGLGTI
                     FVSVMGLALLWLWRGRLFTANWLLWLLLLSAPFPYIATTAGWMTAELGRQPWLVYGLQ
                     RTAEGASPLVHSG"
BASE COUNT          515 a          759 c          748 g          568 t
ORIGIN      
        1 actcgaagga aaaatcacgg ctcagtgggc gtcgctgctg gatggagaat gccggacgat
       61 ccttcgacag aagaagacgg tgcatctcga ctgcgcgaag gtcgatttca tggatgagca
      121 aggcatggag gtgttgaaga acctcccccg gaagaatgtc acccttattg gcgctcctga
      181 gtatgtgacg gagctgctgc acatcggagg tcgctcatga actcggtcgc caccacctat
      241 acagcatctc tgggtcatgc tccatcggtc aaccggaaga cacagtctgc cgagagaccg
      301 ggcctcacga ggagcgaaga tcggctgatc gcccggcttc gacatggtga cgaaggggcc
      361 ttcgacgagc tggtgaatca gcatcacggc tcactgatcc gggtagccct cggctatgtg
      421 gccgaccgtg acgtcgcgga ggaagtcgtg caagacacct ggatgacggt gatcgagaag
      481 cttgacagtt tcgaaggtcg ctcctcgctc cgcacctgga tcttcgggat tctcatccat
      541 aaggcgaaag accgcggggt gcgtgaaaag cgacatacca ccttctccgc gttggaatcc
      601 tatgacgatg acaacgaaga agccgtggac ccggcccggt ttcagcagtc gggggaatgg
      661 gccggccact gggcatttcc gccgcaaccc tgggatgacc ggacaccgga aacactcttg
      721 gcgtcgaaac aagcaaccga ctgcatgcaa caggcgattc aggacctgcc ttcaacgctg
      781 cgggacgtgt tgatcatgag ggatgtcgaa ggcatcgagt cgaccgaaat ttgcgagctg
      841 ctgaagatta ccgaaactaa tctttatgtc cgcctgcatc gggctcgcga gcgagtacgc
      901 caagctgtgg aaacgtacct ggagggtggt caatagaggt gtaagaaaac cagagataga
      961 gggactcagg taatgacacc ctaaccggag agctctctga atgaaagacc acccttcaca
     1021 atggattgtt agtggactca cctgccgcga ggtgactgat cgaacatctg actatctcga
     1081 cgaacgactg ccaatcgtaa cgaagatccg tgtcgccttg cacctcgcgt catgtgcgga
     1141 ctgccgcgcc tatgtgcaac agctcgcgct ggtccgggat gcggcggcac tcctccccaa
     1201 gccgcatctc tcccccatta accagctgcg tctccgccaa cactttgccc gctaccatgc
     1261 tcttttgcag tgatcgaaac gattcgggta tgctgtagca cttcccgtac cgcgcgacgt
     1321 caccatcatg gggctcaacg cccctctgcg ttttgattca tcaacacgga gacttcatgg
     1381 agagcgcgct attttacgac cgtctgcaat ttgcgatcac cgccaccttt cactatctct
     1441 ttccacaatt gaccatgggg ttggcgttgc tgctgttcta cttgaggagc agggcactcc
     1501 ggaccggcga tgagcattac catcgtgtgg ctcggttctg gacgacgatc tttgccttga
     1561 gcttcgcctt cggcgtggtc accggcattc cgctggaatt tcagttcggc accaactggg
     1621 cgaaattctc caacttcgcc ggcggcgtca tcggacagac gttggcgatg gaggggatgt
     1681 tcgccttctt tctggaatcg tcgtttctgg gaatcttgct ctacggagag aaccgattca
     1741 gccgccgcac gcaatggttc gcctcgctga tgctgtttct cgggtcgtgg ctgtctggct
     1801 atttcatact ggccacgaac gcctggatgc aacatcctgt cgcctatact attgcgcccg
     1861 atgggcgctt gttcgtcgac agtctttccg gcttgctcac caatccctgg ctgttctggc
     1921 aattcacgca taatatgacc gcggcggtcg tcaccgcctc gttcgtcatg gcggcggtcg
     1981 gggcgctcta cctgctttcg aacacacacc tggattacgg tcgaacgttt gttcggactg
     2041 gcgtggtggc cggagccgtc gcggccgcgc tgatgatttt ccccacgggc cacgggaatg
     2101 cgaaacaggt gttcgaacat cagccggtca aaggtgcggc cttcgaaggg ttgttccgca
     2161 ccgagcatgg cgccgacatc ctcctggtcg gccagccgaa catggaaacc atgaccatcg
     2221 acaatccgat tgtcgtgccc gatgcgctca gcattctggt gtacaaccgg ctctatgcga
     2281 aggtgaaagg gctcgatgcc tttccgcgga aggactggcc cgataatctg ccgcttcttt
     2341 actattcgta ccacgtcatg gccggcctgg ggaccatctt tgtatcggtg atgggactgg
     2401 ccctgctctg gttgtggcga ggacggctct tcaccgccaa ttggctgctg tggcttctcc
     2461 tcttgtcggc accgtttccc tatatcgcga cgaccgccgg gtggatgacc gctgaactcg
     2521 gccgccagcc ctggctggtc tatggcctgc agcgaaccgc agagggcgcg tcgccgctcg
     2581 tccattccgg
//
LOCUS       DATDNW010000364         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_120833, whole genome shotgun sequence.
ACCESSION   DATDNW010000364 DATDNW010000000
VERSION     DATDNW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120833"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VI359_08690"
     CDS             <1..610
                     /locus_tag="VI359_08690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY6974367.1"
                     /translation="PQYIADLISWTAIGARTTESQTHREMASGVSMPVGFKNGTEGSL
                     QVAVNAMTAARTPHHFVGINADGQTSIIKTMGNPDRHIVLRGGGGKTNYDKEHVAQAE
                     AAVAGEGIARPIMIDCSHDNSHKDHRRQGLVAREVLRQFRDGRQSIMGLMLESNLHPG
                     KQTWQQGKALAHGISITDACLGWDETQALLSELADMIVTRPA"
     gene            661..1440
                     /locus_tag="VI359_08695"
     CDS             661..1440
                     /locus_tag="VI359_08695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY6974368.1"
                     /translation="MLIDTHTHLDDARYDSDREAMIARARDAGVEAFVTIGCDLATSR
                     TAIALADRYPFVFASIGVHPHEVKHIGDMWYDEFRRLAMHEKVVAYGEIGLDYHYNHS
                     SPKDQRNRFREQVALARELKLPVIIHTREAQKDTIAILKEEKASDVGGVFHCFSGDAW
                     LAKDALDLGFYLSFSGVLTFQNAAMLRDIAKTVPLDRLLIETDCPYLTPVPHRGKRNE
                     PAYVSLVARQLAQIFSSDPTLSTDTIGRITTENAKRLFKIA"
     gene            complement(1427..1957)
                     /locus_tag="VI359_08700"
     CDS             complement(1427..1957)
                     /locus_tag="VI359_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY6974369.1"
                     /translation="MALHLIVDGYNLLALLDRASGRVPLHSDMARESLIQELAAYRHR
                     KAHAVTVVFDGWQQGFATERREHRSGIEVIYSRRGEKADQVIQRLAAEFGSDCAVVSS
                     DREVTNYAQAQGALVIEASEFSTKLRGGATTRRIAFKELDTGDNPAPVRTSDKRGNPR
                     KLPKAQRQRNRQLKRF"
     gene            2086..>2492
                     /locus_tag="VI359_08705"
     CDS             2086..>2492
                     /locus_tag="VI359_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974370.1"
                     /translation="MPPALWRIVIASVAVQLVGIHCLVTPGSLDGHVLAASSEVRQEK
                     PRQVRGTHAKASSASLVPAVIQNLRSTASPGLHRLVLDLDRRTRVKKHFIPKSNGVIA
                     EIPNATLSKSAQTKLAGGSIAKPFVITQVSSHSV"
BASE COUNT          541 a          765 c          657 g          529 t
ORIGIN      
        1 gccccagtac atcgcggatc tcattagctg gacggcgatc ggagcgcgca cgacggagag
       61 tcagactcat cgcgaaatgg ccagcggcgt atcgatgcca gtcggcttca agaacggcac
      121 ggaaggcagc cttcaagtgg cggtcaatgc gatgacggcg gcccggaccc cgcaccattt
      181 cgtcgggatc aatgctgacg gccaaacatc gatcatcaag accatgggca acccagaccg
      241 gcacatcgtg ctccgtggag gcgggggtaa aaccaactat gacaaggagc atgtcgcaca
      301 ggctgaagca gcagtcgccg gagaaggcat tgcgcgtccc attatgatcg actgttccca
      361 tgataactcc cataaggacc atcggcgcca aggcctcgtc gctcgtgaag tccttcgcca
      421 atttcgcgac ggacgtcagt ccatcatggg cttgatgctc gagagcaatc tccatcccgg
      481 caaacaaacc tggcagcaag ggaaagcgct ggcccatggt atctcgatca ccgatgcctg
      541 cctcggctgg gacgaaaccc aggctcttct gagcgaattg gccgacatga tcgtcacgag
      601 acccgcctag atattcagtc tcaccttatt gggcctggat gaagtgagaa gcggatttgt
      661 atgctcatcg atacgcacac gcatctcgat gatgcccgct atgacagcga tcgcgaggcg
      721 atgattgcac gtgcacgaga cgccggcgtc gaggctttcg tcacgattgg gtgcgacctg
      781 gccacaagtc gtacagcgat cgcactagcc gaccgctacc ccttcgtgtt cgcttccatc
      841 ggcgtgcatc cgcatgaagt caagcatatc ggggacatgt ggtatgacga gtttcgacga
      901 ctggccatgc acgagaaggt cgtggcctac ggtgaaatcg gtctcgacta tcactataat
      961 cattcgtcgc cgaaagatca gcgtaaccgc ttccgggaac aggtggccct cgcgcgagag
     1021 ctgaaactcc cggtgatcat tcacactcgg gaagctcaga aggacaccat tgccattcta
     1081 aaagaggaga aggcatcaga cgtcggcggg gtgtttcact gtttctcggg agatgcctgg
     1141 ctggcgaagg atgcgttaga tctgggattc tacctttcct tctccggcgt tctgacgttt
     1201 cagaacgccg cgatgttgcg tgacatcgcg aaaaccgttc cgctggatcg cctcctcatc
     1261 gagaccgatt gcccctacct cacacccgtt cctcaccgtg gcaaacggaa tgagccggcc
     1321 tatgtgtccc tggtggcacg gcagctcgca cagatatttt caagcgatcc aactctttcg
     1381 accgacacaa tcggtcgtat cacgaccgag aacgccaaac gtcttttcaa aatcgcttga
     1441 gctgtcgatt tcgctgccgc tgcgcctttg gaagcttccg aggattgcct cgcttatccg
     1501 atgtccgtac gggcgcggga ttgtcccccg tatcgagctc tttgaatgca attcgtctcg
     1561 tcgtcgcccc cccacgaagc ttggtcgaaa actcgctcgc ctcgattacc aatgctccct
     1621 gcgcttgtgc gtaatttgtg acttctctgt ctgaactcac aacggcacag tcgctgccaa
     1681 attcggcagc caagcgttga atgacctgat ccgctttctc gccgcggcgg gaatagatga
     1741 cttcgatccc cgaccgatgc tcgcgccgtt ctgtggcgaa tccttgctgc catccgtcga
     1801 acacgaccgt cacggcatga gctttcctgt ggcggtaggc tgccaactcc tggatcaacg
     1861 actcgcgcgc catgtcggaa tggagcggga cgcgcccact cgcacggtcg agcaacgcca
     1921 gaagattata gccatcgacg atcagatgca gcgccatgac gaaaccctac gctcgactga
     1981 atgccctgtc aattcgtcag accccggtct gttcctgtat tccccttgac aagcacgaga
     2041 acatctgaga aaaaactctt gaatccttca gtttgtggac cacccatgcc accagctctc
     2101 tggcgcatcg ttatagcctc cgtcgcggtc cagctcgttg gcatccactg cctcgtcacc
     2161 cccggttcgc tcgatggtca tgtgcttgct gcatccagcg aagtacggca ggagaagcca
     2221 cgacaggtcc ggggtactca cgcgaaggcg tcttctgcct cactcgtccc cgcggtcatt
     2281 cagaaccttc gcagtacagc atcaccgggt ctgcatcggc tcgtactgga tttagaccga
     2341 agaacacgtg tcaagaagca cttcattcct aaatccaacg gggtcatcgc cgaaattccc
     2401 aacgccactc tcagtaaatc ggcccaaacc aagcttgcag gcggttcaat cgccaaacct
     2461 tttgtgatca cccaggtttc ttcgcattcc gt
//
LOCUS       DATDNW010000365         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_53878,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000365 DATDNW010000000
VERSION     DATDNW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53878"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VI359_08710"
     CDS             <1..237
                     /locus_tag="VI359_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY6974371.1"
                     /translation="IAVFNVDGQYHAIYNVCPHEGGPLNEGRLKGFVVSCPWHDLAFD
                     IRNGQGIDGGGHCIGSYEVRVEGEEIFVGPRRKV"
     gene            296..625
                     /locus_tag="VI359_08715"
     CDS             296..625
                     /locus_tag="VI359_08715"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEY6974372.1"
                     /translation="MDGTTPPRDARIIETVLDRSIHIHLWEDRTRGELWVPTYDPTAL
                     ALVADDYFRVAGNNAVDNGERTFEFQAIRPGTHSVLFEKRMGWKFTAEDRRVFEVVVA
                     DRSSQRS"
     gene            631..1470
                     /gene="dat"
                     /locus_tag="VI359_08720"
     CDS             631..1470
                     /gene="dat"
                     /locus_tag="VI359_08720"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEY6974373.1"
                     /translation="MSDVAFVNGRFMPLAEATVSVEDRGFQFGDGVYEVIRTYRGRPF
                     ELEAHLARLERSVKALDLPQPYSRSQWTQYVLEGINRAAYPEAKIYLQITRGVAPRDH
                     AFPANATATVVMTIRELHVLSEEVKSTGVSAIVIDDIRWGRCDIKSVNLLANVLARQQ
                     AKQARVFETILVKDGHVTEGAVSNVMAVKGGAVMTAPEGPLILSGVTRAVVLELARKD
                     GIPVQEQAIPRSDLYCVDELLLTGTTVEVVGVVKVDGKVIGTGRPGPITQRLAALLRH
                     VTG"
     gene            1588..2064
                     /gene="rimP"
                     /locus_tag="VI359_08725"
     CDS             1588..2064
                     /gene="rimP"
                     /locus_tag="VI359_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY6974374.1"
                     /translation="MKDSRPVVDRLQDIVSPILWTMGLELVDVECVGQGSRTVVRVYI
                     DKPGGVTVSDCERAHVALSPALDVSDPFPHPYTLEVSSPGLDRPFKRLQDYRRAVGTR
                     VSVKLRQPIDGQWRIVGILAEVNAQVMTMTVSEPPPPRSITLDFGGIAETRRVVTF"
     gene            2117..>2469
                     /locus_tag="VI359_08730"
     CDS             2117..>2469
                     /locus_tag="VI359_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY6974375.1"
                     /translation="MNRELIAVIDEIGRQKGIEKSRVIGAIEAALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKTIVDAVTNPKAEISLKEARQYDSEAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKV"
BASE COUNT          546 a          633 c          784 g          506 t
ORIGIN      
        1 attgctgtgt tcaatgtcga cgggcaatac cacgcaatct acaatgtctg cccgcacgaa
       61 gggggcccat tgaacgaagg gcggttgaag ggatttgtcg tgtcctgccc atggcatgat
      121 ctggcgttcg atattcgaaa cggtcaagga atcgatggtg gcgggcattg catcgggagt
      181 tacgaggttc gagtcgaggg tgaagagatt ttcgtcgggc ctcgccgtaa ggtgtagtgc
      241 acgggaagct caaccacgtc ccagcgtgcc gatgaatgtg agagcgaacc acgacatgga
      301 tggaaccaca ccgccgcgtg atgcgagaat catcgaaacc gtactcgacc gatccatcca
      361 cattcacctc tgggaagatc ggacccgggg agagctctgg gtccccacct atgatccgac
      421 ggcgctcgca ttagtggcgg acgactactt ccgtgtagcg ggaaataatg ccgtcgataa
      481 tggagagcga acgttcgagt ttcaggccat acgaccaggg acccactcgg tgttgtttga
      541 aaagcggatg gggtggaaat tcaccgctga agatcggcgc gtcttcgagg tcgtcgtcgc
      601 cgatcgttcc tcgcaacgga gttagtcatc gtgtcggatg tggcatttgt gaacgggcga
      661 ttcatgccgc tggctgaggc caccgtctcg gtcgaggatc ggggatttca gttcggcgac
      721 ggggtctacg aagtcattcg tacgtatcgc gggcgcccat tcgaactgga ggcccatctt
      781 gcccgtctcg aacggagcgt gaaggcgctc gaccttccgc aaccgtattc tcgcagccaa
      841 tggactcaat acgttcttga ggggatcaac cgggcagcct atcctgaagc caagatctat
      901 ctacagatta ctcgcggtgt ggcgccacga gaccatgcgt tccctgccaa cgcaacggcc
      961 actgttgtca tgacgatccg tgaattgcat gtcctcagcg aagaggtcaa gagcaccggt
     1021 gtgtcggcga tcgtgatcga cgacatcagg tggggacgct gcgatatcaa gagtgtgaat
     1081 ctacttgcca atgtcctggc acgccagcag gccaaacagg cccgggtctt tgagaccatt
     1141 ctcgtcaaag acgggcacgt aaccgaaggt gcggtcagca acgtgatggc ggtcaaaggc
     1201 ggagctgtaa tgactgctcc cgaggggccc ttgattctct ccggtgtcac cagagcagtg
     1261 gtgctcgagc ttgcccgaaa agacgggata ccggtccagg aacaggcaat tcctcgatcg
     1321 gatctctact gtgtcgatga gctgctcttg acaggaacca ccgtcgaggt ggtcggtgtg
     1381 gtcaaggtgg acggcaaagt gattggaaca ggccgccctg gtccgatcac gcaacgactg
     1441 gctgctctgt tgaggcatgt gaccgggtag cccccttgct ttgaccgggg tgaaatgcta
     1501 tggtgctttg ctgaaagtgg gctcgggccc acttttttgt ttggcctcca ggaagtgcga
     1561 tccggagggc cgaggctagg ggtgtcggtg aaggattccc gaccggttgt tgatcgcctg
     1621 caagacattg tgtctcctat cctctggacg atggggctgg aactcgtcga cgtcgagtgt
     1681 gtggggcagg gctcacggac agtggtgcga gtctatatcg ataaaccggg tggcgtcacc
     1741 gtcagtgatt gtgaacgggc gcatgtggcg ttgagtccgg ctttggacgt cagcgaccca
     1801 tttccccatc cctatacgct tgaagtgtcc tcgccggggc tggatcggcc attcaaacga
     1861 ctccaagatt atcgccgggc ggtgggcaca cgagtcagcg tcaagctgag acagccgatt
     1921 gacggacagt ggcgcatcgt cgggatattg gcggaggtca acgcacaagt tatgacgatg
     1981 acggtcagcg agccgccgcc gccacgatca atcaccctgg acttcggcgg gattgcggaa
     2041 acacgacggg tggtgacgtt ttagatcgcg gagaagcggg atcccgagac gacgatatta
     2101 cggtggagag taacctatga atcgagaact cattgcagtg atcgacgaga tcgggcggca
     2161 aaagggcatc gagaagtcgc gggtgatcgg cgcgattgaa gcagctctcc aaaccgcggc
     2221 gaagaagcgg tttggccaag ctgaaaacat tcaagtcgaa atcgatccaa agaccggcga
     2281 gatctccgtg gtgtccaaga agactatcgt cgatgccgtc accaatccca aagccgagat
     2341 ttctctcaaa gaagcccgcc agtatgatag cgaagccgag gtgggagatg agatcgggtc
     2401 cttgattgaa atggacgaat tgggccggat cgcggcgcaa acggcgaagc aggtgatctt
     2461 tcagaaagt
//
LOCUS       DATDNW010000366         4188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_673611, whole genome shotgun sequence.
ACCESSION   DATDNW010000366 DATDNW010000000
VERSION     DATDNW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673611"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VI359_08735"
     CDS             <1..313
                     /locus_tag="VI359_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY6974376.1"
                     /translation="TNHFRTTVETGLQIIKALQGHTSGLAVPHFVIDAPGGGGKIPLL
                     PGDYLVHMDEDGVLLKNYENKPFHYPQPKNGSNRELPMVGAHPAWGACSNGDGGGFDK
                     L"
     gene            310..1461
                     /locus_tag="VI359_08740"
     CDS             310..1461
                     /locus_tag="VI359_08740"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEY6974377.1"
                     /translation="MSRATPQGILPYQHITRLITSGAIRSQIPIEDRQIQPASLDLRL
                     GRKAYRLISSFLPELSAITNRLNVLDFYQSDLVMYEMDLTEGAILEKGHVYLVPLLES
                     LRLPKTLRARANPKSTTGRLDVFTRVVTDLNTGFDEIQAGYQGPLYLEVVPRSFAIKV
                     KTGYSLNQIRFVRGEATLPDATLQTLHKQTPLLYHNVSTKKSLGSRDLRTERGLFLRI
                     DLKGDDRVASPIIGYRAKKNSHVIDLSKVSYYAALDFWEPLYRHRQDSLLLEPEEFYI
                     LASKERICVPPGYAAEMVAYEAACGELRTHYAGFFDPGFGFGKGELHGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLDIPSQLYGTGLGSSYQRQGLTLSKHFKT"
     gene            1540..1887
                     /locus_tag="VI359_08745"
     CDS             1540..1887
                     /locus_tag="VI359_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974378.1"
                     /translation="MHNQDSDDDRRMHVAGMMVGTALMFLGFLQVFLSISGGFEINVV
                     PFLIYFGGVAVWANAVIENPTFRYSVIGGALLLGAAFYHYGEVLFWHKQVLFWTTVVI
                     VMYFMFNEPKKPA"
     gene            1898..2581
                     /locus_tag="VI359_08750"
     CDS             1898..2581
                     /locus_tag="VI359_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY6974379.1"
                     /translation="MTISVIIPTLNEARIIADTLSHTANLGFDELIVVDGGSTDGTAA
                     IAEAYAGGVRVIAAPRGRARQLNVGAYASRGDILVFLHADTRLPMTAKQEIVAAFTDD
                     ATVGGRFDVQFDHPSVLGQIISTLMNWRSRWSGIATGDQAIFVRRRTFDQLGGFADIP
                     LMEDIDFSRRLKQLGVMAMLPDHVVTSFRRWDTNGPIKTILLMWGLRFLYWIGVSPHH
                     LQRFYIAVR"
     gene            2589..3335
                     /locus_tag="VI359_08755"
     CDS             2589..3335
                     /locus_tag="VI359_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY6974380.1"
                     /translation="MKPNPGPQHSALSTALVIFAKAPVPGQVKTRLCPPLTQDEAATL
                     HGSFVLDTLERTKTAIAKDRLSVDRYIACLPSASPVFFKILEERHGVRLLDQVGDDLG
                     QRMHRAFTELFGRGYRQVFIVGTDVPTLPLSVYQQAFSLIESHDLVLGPATDGGYYLI
                     GLKRPIPELFTDVPWSTANVLVATRRHASVLGLTVGLLKEWRDIDVADDLRALIQDCA
                     QDMKRPKQERVFSARTAGALQLLDQRLRSR"
     gene            3370..4107
                     /locus_tag="VI359_08760"
     CDS             3370..4107
                     /locus_tag="VI359_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY6974381.1"
                     /translation="MMADRIALITGGAKGIGRGIALDLAAKQWKIAICYRTSAGEADA
                     TAAEISRRGGQPLAVQCDVSDPAAAQQLVKQVEQKWGRIDALINGAGPYHRVNLFEET
                     VAGWNEMFNGNLHPIFYLAQAVAPGMKTRKSGRIINFSMANADQMVAQPDVTAHYIAK
                     AGVLILTRTLAKLLAPHGITVNAISPGFIDSGSAPPEELAGMTKRIPAGYVGTIDDTV
                     AAVRYLLSDDARYVNGANIQLSGAWGV"
BASE COUNT          878 a         1236 c         1204 g          870 t
ORIGIN      
        1 gaccaaccac ttcagaacga ccgtcgagac gggattacaa atcatcaaag ccttgcaggg
       61 ccacaccagc ggcctggccg ttccgcattt cgtcatcgat gcacccggag gcgggggcaa
      121 gattccgttg cttcccggag actatctcgt ccacatggat gaagacggag tcctgttgaa
      181 gaattacgag aacaaaccgt tccattaccc gcaaccgaag aacggctcga accgtgagct
      241 gccgatggtc ggtgcccacc ctgcgtgggg agcgtgcagc aacggcgacg gcggagggtt
      301 cgacaaactg tgagtcgtgc caccccgcag ggcattctcc cctatcagca catcacgcgt
      361 ctgattacga gcggagcgat tcgatctcag atcccgatcg aagatcgcca gattcaaccg
      421 gcgagtctgg acctgcgcct tggccgcaag gcctaccgct tgatcagcag ttttctcccg
      481 gaactgtcgg cgatcactaa tcgactcaat gttctcgact tttatcaatc cgatctggtg
      541 atgtacgaga tggatctcac cgagggggcc atactcgaaa aaggtcatgt gtacctcgtc
      601 ccgctgctgg aaagcctccg gctcccgaag acgcttcgtg cgcgggccaa tcccaaaagc
      661 accaccggcc ggctcgacgt ctttacccgc gtcgtcaccg acctcaacac tggtttcgac
      721 gaaatccagg ccggctacca aggtccgctg tatctggaag tcgtcccgcg atcctttgcc
      781 atcaaagtga agaccggtta ttcgctgaac caaattcgct ttgtgcgcgg cgaagcgacc
      841 ctgccggatg cgacgttgca gacattgcac aaacaaactc cgttgctcta tcacaacgta
      901 tcgaccaaga aatcgctcgg cagccgcgac ctgcgtacgg aacgcgggct gttcctgcgg
      961 attgatttga agggcgacga tcgagtcgcc tcgcccatca tcggctaccg agcgaagaag
     1021 aacagccacg tcatcgacct ttcgaaagtc agctattatg ccgcgctcga cttttgggaa
     1081 ccgctctacc ggcatcgcca ggacagtctc ctattggagc cggaagaatt ctatattctc
     1141 gcgtcgaaag aacggatttg tgtcccccca gggtatgcgg ccgagatggt ggcatacgaa
     1201 gcggcctgcg gggagctgcg gacccactat gcggggttct tcgatccggg atttgggttt
     1261 ggaaaaggcg agctgcacgg gactcaagtt gtgttggagg tccggccgca cgacgtgccg
     1321 tttctgatcc acgatggaca aaccttcttc aaggttgtat acgacaggat gctcgacatt
     1381 ccgagccaac tgtatggcac tgggctcggg tcgtcctacc agcgccaagg gttgaccctc
     1441 agcaaacatt tcaagactta gcgaacaatg gcaccgaccg acctccctac tccgccatcg
     1501 gatgatccta ttcaaccttc cccgccgagg cctacgcgga tgcataatca ggactcagac
     1561 gacgatcggc ggatgcatgt cgccggcatg atggtcggaa ctgcgctgat gttcctcggg
     1621 tttctgcagg tattcctctc gatcagcggc ggatttgaga tcaacgtcgt ccctttcttg
     1681 atttatttcg gaggggtggc ggtctgggcg aacgctgtca ttgagaaccc aacttttaga
     1741 tacagtgtca ttggaggcgc gttactgctg ggcgctgctt tctatcacta cggggaagtg
     1801 ctgttctggc acaagcaggt cctcttttgg accaccgtcg tgatcgtcat gtattttatg
     1861 ttcaacgaac ccaagaaacc cgcttgactt ctgtccgatg acgatctccg tcatcatccc
     1921 caccctgaac gaagcacgaa tcatcgctga tacgctttcg cacacagcca acctcggctt
     1981 tgatgagctc atcgtcgtcg acggcgggag tacggacggg acggcggcca ttgcagaagc
     2041 ctacgcgggc ggcgttcgcg taatcgcggc tccgcgtggc cgcgctcgcc aactgaacgt
     2101 cggggcatac gccagccggg gggacatctt ggtcttcctg catgccgata cccggcttcc
     2161 catgacggcg aagcaagaaa ttgtcgcggc gttcactgac gatgccacag tcggagggcg
     2221 attcgatgtc caattcgatc acccgtccgt acttggccag atcatcagta cgttgatgaa
     2281 ctggcgatcc cggtggagcg gcatcgctac cggcgatcag gccatttttg tgagacggag
     2341 gacgttcgat cagcttggcg ggtttgccga catcccgctg atggaggaca tcgacttcag
     2401 ccggcgattg aaacagctgg gggtaatggc catgttgccg gaccacgttg tgacctcgtt
     2461 tcgccggtgg gataccaatg gtcccatcaa gaccatcctg ctgatgtggg gccttcgctt
     2521 cctttattgg atcggcgtga gtccccatca cctacagcga ttctatatcg cggtgaggta
     2581 gagacacgat gaagccgaat cccggtcctc agcactcggc gctcagtaca gctctcgtca
     2641 tcttcgcgaa agcgccggtg ccgggtcagg tgaaaaccag gctgtgccct ccgctcacac
     2701 aggacgaagc cgccacgtta cacggcagtt ttgtgctcga cacccttgag cgaacaaaga
     2761 ccgcgatcgc gaaggaccgc ctgtctgtgg accgttacat cgcctgcctc ccctcggcct
     2821 ctccggtatt ctttaaaatt ctggaagaac ggcatggtgt ccgccttttg gaccaggtgg
     2881 gggacgattt ggggcagcgc atgcatcgcg cgttcacgga actcttcggc cgcggctatc
     2941 ggcaggtgtt catcgtagga acagacgtcc caaccctgcc gttatcggtg tatcagcagg
     3001 ccttctcgct catcgagtcg cacgatctgg ttcttggccc ggccaccgac ggcgggtatt
     3061 acttgatcgg cctgaaacgc cctataccgg aactctttac tgatgtaccc tggtcgacgg
     3121 caaatgtcct cgtggccacg cgccggcacg cctcggttct tggtcttacc gtcggcctcc
     3181 tgaaggagtg gcgggacatc gacgtggccg acgacctccg ggcattgatc caggattgtg
     3241 cgcaggacat gaagcgaccg aagcaagaac gggtgttctc ggcgcgaact gcgggcgcgc
     3301 tgcagttgct cgaccagcgt cttcgatcga gatgaggaaa tcgacacaga caatcactta
     3361 cgaggtggca tgatggcgga tcgcatcgca ctcatcacgg gaggcgctaa aggcatcggg
     3421 cggggcattg cactggatct cgccgccaaa cagtggaaga tcgcgatttg ctatcgcacg
     3481 agtgccgggg aggccgacgc cacagccgcc gaaatttccc gacgaggtgg gcaaccgcta
     3541 gcggttcaat gcgacgtctc ggaccccgcg gcggcccaac agcttgtgaa gcaggtcgag
     3601 cagaagtggg gccgtatcga cgcgttgatc aatggagccg gcccctatca ccgcgtgaac
     3661 ttgtttgaag aaaccgtcgc gggatggaac gagatgttca acggtaatct ccatccgatt
     3721 ttctacctgg cccaagccgt tgcccctggt atgaaaactc ggaaatcggg ccgtatcatc
     3781 aacttcagta tggcgaatgc ggatcagatg gtggcccagc cggacgtgac cgcgcactat
     3841 atcgccaagg ccggggtgtt gatcctcaca cgcacgctcg cgaaactgct ggctccacac
     3901 ggcatcacgg tcaatgcgat ttcgccgggc ttcatcgatt ccggcagcgc gccgcccgaa
     3961 gaactggcgg gcatgaccaa acgaatcccg gccggctatg ttggcacaat cgatgataca
     4021 gtcgcagccg tgcgctatct gctgtccgac gacgcccggt atgtgaacgg agccaacatt
     4081 caattgagcg gtgcctgggg ggtatgaaag gtagcctgac tatctttctt gctcgcagag
     4141 cccacacgat aaaacagtgg tcgctcgatg cgcgcagtga aagacagt
//
LOCUS       DATDNW010000367         4432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_525673, whole genome shotgun sequence.
ACCESSION   DATDNW010000367 DATDNW010000000
VERSION     DATDNW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525673"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..2454
                     /gene="shc"
                     /locus_tag="VI359_08765"
     CDS             277..2454
                     /gene="shc"
                     /locus_tag="VI359_08765"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY6974382.1"
                     /translation="MKLFRTIFTRLSDGFFASVPEKFRLVTEFSKAPALRLISEKSTT
                     PAPVDAPGRRAGGHSGSQVEAIDDAVRRSQAWFLARQHASEGYWVAELEADTTLTSEY
                     LMLRRFLDRVDLERERKAVRYLRSTQLPDGGWPIYYGGPSEISASVKAYFALKLSGVS
                     AEEPFMMRAKDCILAKGGVVQANVFTKITLALFDQYDWQGIPSMPPEIMLLPKRFYFS
                     IYAISYWSRAVLIPLLIVFANQPVCRISQEESIDELYVTPRPQIRYRQFPPFNKDQGW
                     FTPHNLFVVLDAVLKVYDRMPVSWLREKALHSAATWTLEHIKGSGGLGAIYPAMANSI
                     VALRCLGYQVDDPLVQKALREIEDLEVYDSIGSGEQRVETMHLQPCHSPIWDTSLLVN
                     ALVEAGMPQDHPSLQKACAYLVSRQTKTVGDWKFSSPEAEPGGWYFQFENELYPDVDD
                     SAVVLMALSKVRMAQTAELQDSIRRGTNWVLAMQGSDGGWGAYDKDNNRIVFNYIPFA
                     DHHALLDPSTADLTGRCLEMLAALGYDQTHSAVAPALAFLKREQEEDGSWYGRWGVNY
                     IYGTWSVLAGLRAIGEDLSQPYIRRAVAWLESKQNPDGGWGESCLSYGESEWTGKGES
                     CPSQTAWALMALMSAGVTDAFSVVRGIQYLLRHQMKDGSWEEVRHTGTGFPRVFYLRY
                     HWYCQYFPLWALAMYRNLRTRGRMRADDIRKHVVTSGYYRSDR"
     gene            2451..3290
                     /locus_tag="VI359_08770"
     CDS             2451..3290
                     /locus_tag="VI359_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974383.1"
                     /translation="MTIAPAQSIGSVHVHSTSAVTRIAVFVATCWELRALRSALPLDE
                     TAILGGVRCGTAERNGRLYYVVQTGVGPARASKVARSILDEGAWALVLSTGFACALAP
                     AGVGDLIIGTEVFSVFHDGQWIRREGLLSCSQEQHLRFLSVGREAGIPLKSGRIVSTP
                     RIIWQADEKRAIHDLTDAVALDMESAALAAVAHEKGIPMAIVRTVSDLVTEDLPLDFN
                     LFLRPTGWLKGLWALIGHPSSFAGVNRLRRQSQVAADRLTDFFKGYIEKSNGVQTAAG
                     DIR"
     gene            3287..4057
                     /locus_tag="VI359_08775"
     CDS             3287..4057
                     /locus_tag="VI359_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEY6974384.1"
                     /translation="MTNLIEHLGTAVLALIREMGRMLIFVVSAFAWLIRPPLRAIQII
                     KQLHFIGYKSTFVVVLTATFTGMVLALQGYYSLRKFGAEGWLGSAVALSMIRELGPVL
                     AALMVTARAGSAMTAEIGIMRITEQIDALDTMAISPLQYLIAPKLVAGLIGVPLLVAI
                     FDVVGIYGGYLVGVDLLGVNAGSYWNSIQSSVEWKDVYGGILKSISFGLIVSWICCYK
                     GFYTKQSAEGLGTATTEAVVLSSVVILVWDYFLTSVLM"
     gene            4061..>4432
                     /locus_tag="VI359_08780"
     CDS             4061..>4432
                     /locus_tag="VI359_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6974385.1"
                     /translation="MIKLIGVEKTLGGQPVLQGVDLSIPQGKLTTVIGRSGEGKSVLL
                     KHMIGLLQPDRGEVWIDDVEISRLKGRKLNDVRKRFAMLFQGAALFDSLTVFDNVAFP
                     LREKLRMKGLEVTKRVDEKLEQ"
BASE COUNT          894 a         1150 c         1341 g         1047 t
ORIGIN      
        1 attgttcggg gtgaccatgg aagagccggg tatctcaaaa cttgtctccg tccgattgac
       61 tgatttgcaa ccggcctagt cgatgatcta acaaaaatct tcgtagttca gaggacttcc
      121 actccttcaa ttcttgactt gggggagggc gtctgttata agcccaacga caggcagctc
      181 cccgagtgtg tggacgtgtt tcgtctcgtc ttctcaacgg agccggtcgt tctcgcgtat
      241 ccgtctgcct tgtgagtttc ccagattaga agcacgatga aactgtttcg aaccatattc
      301 acccgtctgt cggatggttt cttcgcgtcc gtcccggaaa aattccgcct cgtaacggaa
      361 ttctcaaagg ccccggcctt acgcctcata tcggaaaaat ctacgactcc tgcacctgtc
      421 gatgcgccgg gtcgacgcgc tggcggtcat tcaggcagtc aagtcgaagc gatcgatgat
      481 gcggtccgtc ggagtcaagc gtggttcttg gcacggcagc atgcgtccga aggctactgg
      541 gtcgctgaac ttgaagcgga cacgacgctg acatccgaat acctcatgct tcgaagattt
      601 ctcgatcggg ttgatctcga acgagaacgc aaggcggtgc gctatctccg ttcaacgcaa
      661 ttgccggacg gtggatggcc gatctactac ggaggtccct cggagatcag tgcgtcggtc
      721 aaagcctact tcgcattgaa gctcagcggg gtatcggcgg aagagccctt catgatgcga
      781 gcgaaggact gcatcctcgc gaaaggcgga gtcgtccaag cgaacgtgtt cacgaagatc
      841 acgctggcgc tatttgatca gtacgattgg caaggcatcc cgagcatgcc cccggaaatc
      901 atgctcttgc cgaagcggtt ttatttcagc atctatgcga tatcgtactg gtcgcgcgcg
      961 gtcttgattc cattgctcat tgtgttcgcg aaccagcctg tctgccgcat ctctcaagaa
     1021 gagagtattg acgaactcta tgtcaccccg cggccgcaga ttcggtatcg ccagtttcct
     1081 ccgttcaaca aggatcaggg gtggtttacc ccacataatc tttttgtggt gctggacgcc
     1141 gtgctgaaag tgtacgaccg gatgccggtc agttggctac gtgagaaggc tctccacagt
     1201 gcggcaacat ggacgttgga gcacatcaag ggaagcggcg ggctcggggc gatctatccc
     1261 gccatggcga attcgatcgt ggcgctccgg tgtctgggct atcaggttga cgatccgctc
     1321 gtgcagaaag cgctccgcga gattgaggat ctggaagtct acgacagtat cgggagcggc
     1381 gagcagcggg ttgagacgat gcatctccaa ccctgccatt ctccgatctg ggatacttcg
     1441 ctgttagtga atgccttggt cgaggccggg atgccgcagg accatccgtc gctgcagaag
     1501 gcctgtgcgt atctcgtatc caggcagacg aagacagtcg gggattggaa gttctcatct
     1561 ccggaagcag aaccgggcgg ctggtatttc cagtttgaaa acgaactgta tcccgatgtg
     1621 gacgactccg ccgtggtcct gatggcgtta tccaaggttc gcatggctca gaccgctgag
     1681 ctgcaggact ccatccggcg aggcaccaat tgggtgctcg ccatgcaagg gtccgacggc
     1741 gggtggggcg cctatgacaa ggataataat cggatcgtct tcaattatat tccgtttgcc
     1801 gaccatcatg cgttgctcga ccccagtacg gcagacttaa ccggacgttg cctcgaaatg
     1861 ctggctgcgc ttggctatga ccagacccat tccgccgtgg caccggcctt ggcgttcctc
     1921 aagcgcgagc aggaggagga cggtagttgg tatggccgtt ggggggttaa ttacatctat
     1981 ggtacttggt ccgtgctggc cggtttgcgg gcgatcggtg aagatctgtc acagccgtat
     2041 attcgccgtg cggtcgcatg gctcgaatcg aagcagaatc cggacggtgg ctggggtgag
     2101 tcctgtctgt cctatggaga gtccgagtgg acaggtaaag gagagagttg cccatcacaa
     2161 accgcctggg ctctcatggc gttgatgtct gcgggtgtca cagatgcctt cagtgtggtt
     2221 cgagggattc agtatctcct tcggcatcaa atgaaggatg ggtcatggga agaagtgcgc
     2281 cataccggca ctgggttccc gcgcgtgttc tacttgcgct atcactggta ttgccaatac
     2341 tttccgctct gggcgctcgc catgtatagg aacttgcgga cgcgcggaag gatgcgcgcc
     2401 gatgacatac gcaagcatgt ggtgacctcc gggtactatc ggtcggatcg ttgacgatcg
     2461 cgccagcgca atccatcggc tctgttcatg tccattccac ctctgctgtg acacgcatcg
     2521 cagtattcgt cgccacttgc tgggaactcc gcgcgcttcg ttctgctctt ccactcgacg
     2581 agacagctat tcttggaggc gtccgctgtg ggacagcgga acggaacggg cgcctctact
     2641 acgtggttca aaccggcgtc ggtcctgctc gtgcgagtaa ggttgcccga tccatactcg
     2701 acgagggtgc atgggcgttg gtgctgtcga cgggatttgc ctgcgcgctg gcgccggccg
     2761 gtgtcgggga tctcatcatc gggacggaag tcttttcggt ttttcacgat gggcaatgga
     2821 tcaggaggga aggtcttctt tcgtgctcgc aggagcagca tcttcgtttt ctttccgtgg
     2881 ggcgagaagc cgggataccc ctgaagagcg gcaggatcgt gtcgaccccc cgaattatct
     2941 ggcaggccga tgaaaaacgg gccatacacg atctcacgga cgctgtcgcc cttgatatgg
     3001 agagtgctgc gttggcagcc gtcgcgcatg agaagggtat tccaatggct atcgtacgca
     3061 cggtgtccga tcttgtaacc gaagatctgc ctcttgactt caatctcttt ctcaggccca
     3121 ctgggtggct caaggggttg tgggcgttga tcggccatcc atcgagcttt gcgggtgtaa
     3181 atcgattgcg aagacagagt caggtggccg ccgaccgatt gactgatttt ttcaagggct
     3241 atattgagaa gtccaacggc gttcaaacgg cggcgggaga catccgatga cgaatctcat
     3301 tgagcatcta gggacggctg tattggccct catccgagaa atgggaagga tgctcatctt
     3361 tgtcgtttcc gcatttgcat ggctcatccg tccgccgctg cgcgccatcc aaatcatcaa
     3421 acaattgcat tttatcggct acaaatcgac cttcgtcgtg gtcctgacgg ccacgtttac
     3481 cggcatggtc ttggcgctgc aagggtacta ctcgttacgg aagttcggag cggaaggatg
     3541 gctcggttca gccgttgcgc tcagtatgat tcgagaatta gggccggtcc tagccgcact
     3601 catggtcacg gcccgggcgg ggtccgccat gaccgctgaa atcgggatca tgcgaatcac
     3661 cgagcagatc gacgccctcg atacgatggc aattagtccg ctgcaatacc tcatcgcccc
     3721 caaactggtc gctggtctga tcggggtccc attgctcgtc gcgatttttg atgtggtcgg
     3781 gatctacggc ggctatctgg tcggagtgga tctcttgggg gtgaacgcag gatcatattg
     3841 gaattcgatt cagtcatcag tggaatggaa ggacgtctat ggtggcatcc tgaaatcgat
     3901 tagcttcggg ctcatcgtca gctggatctg ctgctacaag ggtttttaca cgaagcagag
     3961 tgccgaagga ttaggaaccg ccacaactga ggccgtggta ttgtcgtcgg tcgtcattct
     4021 cgtgtgggat tatttcctca cctcggtatt gatgtaagcc atgatcaagc tgatcggggt
     4081 tgaaaagacg ttgggaggac agccggtgtt gcaaggagtg gatttgtcca ttccacaggg
     4141 caaactcacg acggtgatcg ggcggagcgg ggaaggcaaa agtgtcctcc tgaaacacat
     4201 gatcggtttg ttgcagcctg accgcgggga ggtctggatc gacgatgtgg agatatcgcg
     4261 attgaaaggg aggaaactca atgatgttcg gaaacgcttc gcgatgttgt tccagggggc
     4321 tgcgctcttc gattcattga cggtctttga caatgtcgcg tttccgctgc gggagaagct
     4381 ccggatgaaa ggtcttgagg tcacgaagcg cgtcgatgaa aagctcgagc ag
//
LOCUS       DATDNW010000368         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_55022,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000368 DATDNW010000000
VERSION     DATDNW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55022"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VI359_08785"
     CDS             <1..374
                     /locus_tag="VI359_08785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974386.1"
                     /translation="NAYPGRVFQARISNIGPVLDPNIRTAKVRLELQNPGLMRIGMFV
                     TATFHGQSQEVRAAVPADAVLHLHDRDWVYVPAGEGKFRRVEVAAGTMLPQHMQEIVS
                     GIRPGQQVVSNALVLQNTVEQ"
     gene            371..>2132
                     /locus_tag="VI359_08790"
     CDS             371..>2132
                     /locus_tag="VI359_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6974387.1"
                     /translation="MIRAVVDYALNNRVLVLAVGLLLLIWGAISFHGLPVEAYPDVAN
                     NYVQIITQWPGRAAEEVEQQVTIPIEIVMNGLPHLDHLRSTSLFGLSSLMLIFDDQSE
                     NDWNRQKVLERLSQVTLPAGLQPQIGTDYSPVGQIYWYTLKSTNPRYDLMELKSLEDW
                     VLEKQFKSVPNVVDVVSFGGITREYQVRIDPDKLVSYGLSIGQVEQQLAGNNVNAGGS
                     FVEAGLQQINVRAVGLYNSSVDIGQTVIKTQNGTPIRIRDVGAVLQGPKIRLGRIGKA
                     IHRADGHVIDNDDVIEGIVLLRKGADSDSTLIAIHDKVKELNEHILPPGVRVVPFLDR
                     SDLVHYTTHTVLHNLTEGIILVALILFLFLGNARGALIVALTIPFSLLFASICLQLNR
                     IPANLLSLGALDFGMVVEGAVVMVENIVRYLGRDREPQSGLGPIREAAHEVQRPVFYA
                     IAIIITAYVPIFTLQRVEGRLFKPMAWTVSFALLGSLIFSMLMAPVLSSFLFGSHVRE
                     WHNPAMIAITRAYRKALHRAIAFRWVTVGIAAATVALSVYLTMSGLIGSEFLPHLDEG
                     AIWARATLAPSTGPTEGSRVM"
BASE COUNT          404 a          662 c          620 g          446 t
ORIGIN      
        1 tcaatgctta tccgggaagg gtctttcagg cgcgcatcag caacatcggc ccggtcctcg
       61 accccaatat ccgcacagcg aaggtgcgcc tggaattgca gaaccccggg ctcatgcgca
      121 tcggcatgtt cgtcaccgcg acattccatg ggcagagcca ggaagtgcgc gcagccgttc
      181 ccgcggacgc cgttctgcac ctgcatgacc gtgattgggt ctacgtgccg gccggcgaag
      241 gcaagttccg ccgcgtggaa gtagcggcag gcaccatgct tccccaacac atgcaggaaa
      301 tcgtgtccgg tatccggccg ggccagcagg ttgtgtccaa cgccctcgtg ttgcagaaca
      361 cggtggagca atgatccgcg ccgtcgtcga ttacgcgctc aacaatcgcg ttctagtgct
      421 ggcagttggg ttgttgctgc tgatctgggg cgccatctcg ttccacggcc tgccagtgga
      481 agcttacccc gacgttgcca ataactatgt gcagattatt acgcaatggc ccggccgcgc
      541 cgccgaagag gtcgaacagc aggtcaccat tcccatcgag atcgtgatga acggccttcc
      601 acacctggat catttgcggt ccacctcgct gttcggtctc tccagcctga tgctgatctt
      661 cgatgaccag tccgagaacg attggaaccg gcagaaagtg ctggagcggc tgtcgcaggt
      721 cacgctccct gccggactcc agccgcagat cggaaccgac tacagccccg tcggccagat
      781 ctactggtac acgctgaaga gcacgaaccc caggtacgac ttgatggagc tgaagtccct
      841 tgaggactgg gttctcgaaa aacaattcaa gtcggttccc aatgtcgtgg acgtagtgag
      901 cttcggcgga attacgcgcg agtatcaggt acgcatcgac ccggataagc tcgtgtccta
      961 cggtctcagc atcggtcagg tcgaacagca gttagccggc aacaatgtga acgccggcgg
     1021 cagcttcgtc gaagccgggc tccagcagat taacgtccgt gccgtcggcc tgtacaacag
     1081 ctcggtcgat atcgggcaga cggtcatcaa gacccaaaac gggactccaa ttcgcattcg
     1141 ggatgtcggc gccgtgctcc aggggcccaa gatccgcctg ggccggatcg gcaaggccat
     1201 tcatcgcgcc gacggccacg tcatcgacaa tgacgacgtg atcgaaggca tcgtgctgct
     1261 gcgcaagggc gctgactccg attcgacgct gatcgcgatt catgacaaag tcaaagaact
     1321 caacgagcat attctgcccc ccggtgtaag ggtcgttccg ttcctggatc gcagcgacct
     1381 ggttcactac accacccaca ccgtcctgca caacctcacc gagggcatca tcctggtcgc
     1441 cctgatcctg ttcttatttc ttgggaatgc ccgcggcgcg ttaatcgtgg cgctgaccat
     1501 cccgttctcg ctgttgttcg catcgatctg ccttcagctc aaccgcattc cggcgaatct
     1561 gctgtcgctc ggtgcattgg atttcggcat ggtggtggaa ggcgcggtgg tcatggtcga
     1621 gaacatcgtt cgctacctgg gccgtgaccg ggagccgcaa tcgggcctgg gaccgattcg
     1681 cgaggccgct cacgaagtgc aacggccggt gttttacgcc atcgccatca ttatcacggc
     1741 ttacgtaccc attttcacgc tacagcgcgt ggagggccgg ctgttcaagc ccatggcgtg
     1801 gaccgtatcg ttcgcgctcc tgggttctct catcttttcg atgctcatgg cgcccgtgct
     1861 ttcgagcttt ctgtttggca gccacgtccg cgagtggcat aacccggcga tgatcgccat
     1921 tacccgcgcc taccgaaagg cattgcaccg ggccattgca tttcgctggg tgacggtcgg
     1981 gatagcggcg gctactgtgg ctctgtctgt ctatctcaca atgagcggct tgatcggctc
     2041 cgaattcctt ccgcatctcg atgagggcgc catttgggct cgcgcaactc tggcgccgag
     2101 caccggaccc accgagggct ctcgcgtgat ga
//
LOCUS       DATDNW010000369         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_745711, whole genome shotgun sequence.
ACCESSION   DATDNW010000369 DATDNW010000000
VERSION     DATDNW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745711"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="thrS"
                     /locus_tag="VI359_08795"
     CDS             <1..950
                     /gene="thrS"
                     /locus_tag="VI359_08795"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY6974388.1"
                     /translation="LKPMNCPYHIMIYKSHLRSYRDLPVRYGELGTVYRYERTGVLHG
                     LLRVRGFTQDDAHLFCRPDQIETEVSRVLDFTFFILRTFGFTEFEVFLSTRPKESVGA
                     DEHWTLATSALEAAIKSRNIAYHLDPGGGAFYGPKIDIKIKDALGRSWQCSTVQVDFN
                     NPERFELSYIGEDGKAHQPIMIHRALMGSIERFFGILIEHYGGAFPTWLAPVQAVVIA
                     ITDNQREFVANVVAEMKAAGFRVEADLRNEKIGLKIREAEKAKTPFMFVVGDREVQSG
                     SLSVRGRSGANLGTMTVAGALDLLRAEANKAGQQLSPTS"
     gene            961..1470
                     /gene="infC"
                     /locus_tag="VI359_08800"
     CDS             961..1470
                     /gene="infC"
                     /locus_tag="VI359_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY6974389.1"
                     /translation="MVPKLRVNREIRVREVRVIGPEGEQLGILPTPEAFKQAQETGYD
                     LVEVAPTSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIK
                     IRQIKGFLEEGNKTKVVLTYRGREMANQEMGRVVMNSVIAELSDTGTIEYAPRMEGRS
                     LIMIVAPKG"
     gene            1505..1702
                     /gene="rpmI"
                     /locus_tag="VI359_08805"
     CDS             1505..1702
                     /gene="rpmI"
                     /locus_tag="VI359_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY6974390.1"
                     /translation="MMKMKSHSGASKRFHKTGTGKFMRKKAGKRHILTSKNRERKRRL
                     SGSAVVDATKLKALGRLLPYL"
     gene            1740..2108
                     /gene="rplT"
                     /locus_tag="VI359_08810"
     CDS             1740..2108
                     /gene="rplT"
                     /locus_tag="VI359_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY6974391.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFINALKKANVLLDRKVLSDMAIKD
                     ATGFEQLIGLAKQHLTPATA"
     gene            complement(2162..>3398)
                     /gene="pheT"
                     /locus_tag="VI359_08815"
     CDS             complement(2162..>3398)
                     /gene="pheT"
                     /locus_tag="VI359_08815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY6974392.1"
                     /translation="YELVKPDEARFTPLGMETVMTLSEILMVHPKGLEHGHLLAGQDR
                     MPILRDAKKQPLSFPPIINSREIGEVRVGDDELFVEVTGTDQSMVVLTLNIFAANLAD
                     RGATIEPIEVRYPKNTPLGKRVITPQDLGKRRTIPIKTIEQALGQEIGTKTVKQALEA
                     YGYDVSAGSGTVKAKLPPYRHDLMHAMDVVEDVAMSRGYAEFSPLMPAQFTVGGLSRI
                     EQTSDRARDLMVGLGFQEIISNILGSPEHYRDAMRLGDTAWGEMVEVDNVMSLSFSCL
                     RQWMLPSLLRVEAASSRAFYPHRLFEAGDVAIPDRTHELGSRTETVLGAMMAHATAHF
                     SEIHSCLDVLFYHLGKEYSLEPVQHPSFLEGRAGRIVVSGKPLGVIGEVHPEVLEQWQ
                     IAVPVVAFDVNLSQLTESQ"
BASE COUNT          816 a          945 c          974 g          663 t
ORIGIN      
        1 agctgaagcc gatgaactgc ccctaccaca tcatgattta caaatcccac ctgcgcagct
       61 atcgcgacct ccctgttcgg tacggcgaac tcgggacagt ctatcgctac gaacggaccg
      121 gcgtcctgca cggcttgctg cgcgtgcgcg gctttaccca ggacgatgcc catctgtttt
      181 gccggccgga ccagatcgaa accgaagtca gccgcgtact ggatttcact ttcttcattc
      241 tacgcacgtt cggattcacc gagttcgaag tatttctgtc gacgaggcct aaagagtcgg
      301 tcggagccga cgaacattgg accctcgcaa ccagtgcgtt ggaagcggcg atcaagagtc
      361 gaaacattgc ctatcaccta gacccaggcg gcggtgcgtt ctatggtccg aaaattgaca
      421 tcaaaatcaa ggatgcactg ggccgttcct ggcaatgttc gaccgttcaa gtcgacttca
      481 ataacccgga acggttcgag ctgagttaca ttggcgaaga cggcaaggcc caccaaccga
      541 tcatgatcca tagagccttg atgggctcga tcgagcgatt cttcgggatt ctgatcgagc
      601 attatggcgg tgcatttccg acgtggttgg cgccggtgca agccgtggtc atagccatta
      661 cagacaacca gcgtgaattt gtcgcgaacg tagtcgcaga aatgaaggcg gccggattcc
      721 gcgtcgaggc agacctccgc aacgagaaga tcgggttgaa aatccgcgag gcggaaaagg
      781 ctaagacgcc gttcatgttc gtcgtcggcg atcgggaagt gcagagcggg agcctctccg
      841 tcaggggacg gagcggcgca aatctcggca caatgaccgt cgccggcgca ttggatctgc
      901 tcagggccga agccaataag gccgggcagc aactttcacc cacgtcatga gaggtggcct
      961 atcgtcccca aactacgcgt aaaccgcgaa attcgcgtcc gtgaagtcag agtgatcggt
     1021 ccagaaggcg agcagctcgg cattctaccc actcctgagg cgttcaaaca ggcgcaagag
     1081 accggatatg atctggtgga agtggccccc acgtcggtgc cgcctgtgtg ccggatcatg
     1141 gattatggga agtacaagta cgaactcagc aagaaggacc atcagagccg acggcaccaa
     1201 aaatcgacgc aggtcaagga aatcaaattg cgccctcgga cggataaaca cgatttggag
     1261 atcaagatac gccaaatcaa aggattcttg gaagagggca acaagacgaa ggtggtcttg
     1321 acctatcgcg gacgcgagat ggccaatcag gaaatgggac gggtcgtcat gaattcggtg
     1381 atcgcggagt tgtcggacac cggcaccatt gagtacgcgc cacgaatgga agggcgcagc
     1441 ttgatcatga tcgtcgctcc taaaggctag gacagaacgc aggaacgttt aaccaaagga
     1501 ttgcatgatg aaaatgaagt cacatagcgg agccagcaaa cggtttcata agaccggcac
     1561 ggggaaattc atgcggaaga aagccggcaa gcggcatatt ctgacgagca agaatcggga
     1621 gcggaaacgg aggctgagcg gatccgccgt cgttgatgcg accaaattga aagccctcgg
     1681 tcggttgctg ccgtatctat aacgttgatg atgcatcgta actaaaagga gtacgagtca
     1741 tgccacgcgc aaaaggtggg ccaaaaacga gagcgagacg gaagaaacgg ctgaagctgg
     1801 cgaaaggaca gtacggtgcc aagagccggt tgttccgctc ggccacggaa tcggtcgata
     1861 agggacaaca gtacgcctat accgggcgca agaatcggaa gcgagacttc cggcaactgt
     1921 ggatcgcgcg cattagcgcg gccacgaggg cgcacggtct cacctacggc cgttttatca
     1981 atgcgttgaa aaaagcgaat gtgctactgg atcggaaagt gctctccgat atggccatca
     2041 aggatgccac cggattcgag caactaatcg gcctcgccaa gcagcacctg accccggcaa
     2101 cggcctagtc ttcaaggact cccgaaaaag agcggagagc gaccgaccgt cgctctccaa
     2161 ctcactggct ctcagtgagt tgagacaggt tgacgtcgaa agccacgacc ggcacggcga
     2221 tctgccactg ttcgaggact tcggggtgga cttcaccgat gacgccgaga ggcttgccag
     2281 agacgacaat cctccccgca cggccttcga ggaaagacgg atgttggacc ggctccaggc
     2341 tgtactcctt ccctaaatgg tagaacagca catccagaca ggaatggatt tcagaaaaat
     2401 gcgcggtcgc atgggccatc atggcgccca aaacggtttc cgtcctggag cctagttcat
     2461 gtgtgcgatc gggaatcgcc acatcgcccg cttcgaacaa acggtgcggg taaaaggccc
     2521 ggctcgaagc cgcttcgacg cgcagcagtg agggcagcat ccactgccgc aggcaggaga
     2581 agcttaacga catcacgttg tcaacttcca ccatctctcc ccatgccgtg tcccccagac
     2641 gcatcgcatc gcgatagtgc tccggcgacc caagaatgtt ggagatgatt tcttgaaacc
     2701 ccagcccgac cattaagtct ctcgcacgat ctgaggtttg ctcgattctc gacagtccgc
     2761 ccacggtaaa ctgtgccggc atcagcggag agaactctgc atagccgcga ctcattgcca
     2821 cgtcttccac gacgtccatg gcgtgcatga gatcatggcg atagggcggc aacttcgctt
     2881 tcacagtccc gcttcccgct gagacgtcat agccatacgc ttcgagagcc tgcttcactg
     2941 tcttcgtgcc gatttcctga ccgagtgcct gctcaatggt tttaattgga atcgttctgc
     3001 gtttcccgag atcttgcggc gtgatgaccc gttttcccaa gggcgtgttt ttgggatagc
     3061 gcacttcgat tggctcgatg gtcgccccgc gatcggccag attcgcggca aagatgttca
     3121 aagtcagtac gaccatcgac tggtcggttc ccgtcacttc cacgaagagc tcgtcgtcgc
     3181 ccacccgcac ttcgccgatc tcccggctgt tgatgatcgg cggaaaggac aagggttgct
     3241 tcttcgcatc gcggagaatc ggcattcggt cctgcccggc gaggaggtgg ccatgttcca
     3301 atcctttggg atggaccata agaatttccg acagggtcat cacggtttcc atccccaatg
     3361 gagtgaatcg cgcctcgtct ggcttgacca gttcatag
//
LOCUS       DATDNW010000370         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1278753, whole genome shotgun sequence.
ACCESSION   DATDNW010000370 DATDNW010000000
VERSION     DATDNW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278753"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VI359_08820"
     CDS             <1..455
                     /locus_tag="VI359_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY6974393.1"
                     /translation="RFVELLTETCQALALAICRDGEGVTKVVKIDVAGADTAHQARQI
                     AQTVATSNLVKTALFGEDANWGRVMAAIGRARVPVVPEKVSIWFDGVPMVRRGVGLGS
                     VAEQRIAKVFKKKEFTIAVRLGAGRHRSHMWTTDLSYDYVRINASYRS"
     gene            681..1454
                     /gene="rpsB"
                     /locus_tag="VI359_08825"
     CDS             681..1454
                     /gene="rpsB"
                     /locus_tag="VI359_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY6974394.1"
                     /translation="MGVVSIKELLEAGVHFGHQTNRWNPKIKKFLFGERNGIYIIDLQ
                     QTVARMEQAYAFVRDTVASGQPVLFVGTKRQAAEILEEEAKRANMFFVNQRWLGGMLT
                     NFQTIRRSIDKMKKMEATLLDPAQHGLTKKEISQMQKEIVKLQKYLTGIKNLRGLPGA
                     VFILDTRVEHIAVMEAARLEIPVIAIVDTNCDPDRITYPIPGNDDAIRSIKLITSRIA
                     DACIEGAHLRTQRDEAEFQSAPAGDAKPAAMSLESVPAS"
     gene            1515..2111
                     /gene="tsf"
                     /locus_tag="VI359_08830"
     CDS             1515..2111
                     /gene="tsf"
                     /locus_tag="VI359_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY6974395.1"
                     /translation="MAGTSQLVKELREKTGAGILDCQKALAENGDNIEKAIDFLRQKG
                     LAAAQKKAGRETNQGLVHAYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPSYVKREEIPGSIIDKERMIYEGQAKEMGKPPATWPKIIEGKLEKFYQESCLIEQ
                     AFIKDPAVTIKDLLSQKIAKIGENINIRRFTRYQLGEV"
     gene            2108..2833
                     /gene="pyrH"
                     /locus_tag="VI359_08835"
     CDS             2108..2833
                     /gene="pyrH"
                     /locus_tag="VI359_08835"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY6974396.1"
                     /translation="MSSAKYRRILLKVSGEMLAGEQGYGIQPSVLEGLAEEIASVVAL
                     DVEVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERKGVMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAAALRAMEIGAEVIMK
                     GTKVDGIYDADPVKNPSAKKYTEISFLSILNQNLKVMDATAISLCMDNNLPLIVFDLK
                     NQGNFKRVTTGEPIGTIVTAKPR"
     gene            2851..>3313
                     /gene="frr"
                     /locus_tag="VI359_08840"
     CDS             2851..>3313
                     /gene="frr"
                     /locus_tag="VI359_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY6974397.1"
                     /translation="MSNPAAIRQKTTEKMEAALEHLKHDLAGLRTGRASVALLDGVRV
                     DYYGTMTPLKQVANLSTPEARLITVQPWEPQLIKEIEKAISASGLGITPSNDGKIIRI
                     PLPPLTEERRKELTKICKKHGEDTKVHIRGFRREANEELKKLQKDAKLTEDE"
BASE COUNT          847 a          890 c          940 g          636 t
ORIGIN      
        1 gtcgatttgt tgaactctta acggagacct gccaagcctt ggctctcgct atctgccgcg
       61 atggagaagg cgtaacaaag gtcgtgaaga tcgacgtcgc cggggctgat actgcccacc
      121 aggcccgtca gattgcccaa actgtcgcaa cgtcgaactt ggtcaagacc gcgctcttcg
      181 gcgaggatgc caattggggt cgggtgatgg ctgcaatcgg gagagccaga gtgcctgtcg
      241 ttcccgaaaa agtcagtatc tggttcgacg gagtccccat ggtcaggcgc ggcgtcggac
      301 ttggctcagt ggccgagcag cgcattgcca aggtctttaa gaagaaggaa ttcaccattg
      361 ccgtccgcct cggtgctgga cgccatcgga gccacatgtg gacgacggac ttgtcatacg
      421 attacgtgag aattaacgcg agctaccggt cctgaggagc ggttacctga agccttgcaa
      481 actcataggt agtatgctag cgtaccaagg ctttgggctt gtaagtgata gcctccgccg
      541 agtcgaatcc cgcatcggac ggagaaattt ttaaaatata cgtcagcgtc acatgacgct
      601 gcggggcttg agaataaggg acatgtgtcc ctcgcctctt ctaatgaggc gctctgcaag
      661 ccttgagagg aggtgaaggc atgggagtgg tttcaatcaa ggaattattg gaagccggtg
      721 tccattttgg acatcagacc aaccggtgga atccgaagat caagaaattc ctctttggag
      781 aacgaaacgg catttacatt atcgatctac agcaaacggt tgctcgtatg gaacaggcct
      841 atgccttcgt ccgcgacacg gtcgcctcag gccagcccgt cctgtttgta ggcacgaaac
      901 ggcaagccgc cgaaattttg gaagaagaag ccaagcgcgc caatatgttt ttcgtcaacc
      961 agcgctggct gggcgggatg ctcacaaact tccagaccat ccgccgaagt atcgacaaga
     1021 tgaaaaaaat ggaggcgacg ctgctggatc ccgcgcagca tggcttgacc aagaaagaaa
     1081 tctctcagat gcaaaaagag atcgtcaagt tgcaaaagta cttgacgggc attaagaacc
     1141 tgcgcggcct tccgggagcc gtctttattc tcgacacgag agtcgagcac attgccgtga
     1201 tggaagcggc acgcctggaa ataccggtca ttgcgatcgt ggatacgaat tgcgatcctg
     1261 accgcatcac ctaccccatt cccggcaatg acgatgccat ccgctcgatc aaactcatta
     1321 cgtcccgcat tgctgacgcc tgtatcgaag gggcccattt gcgcacgcaa cgggatgagg
     1381 cggagtttca atccgctcct gccggcgatg ccaagccagc tgccatgagc ttggagagcg
     1441 taccggcgtc gtagggtcgg gcacgcgagc ggtctatcat ctttaaaccg tcgaatagag
     1501 aagggcgctg acgcatggca ggaacaagtc aactggtcaa agaactacgg gaaaaaacag
     1561 gggccggcat acttgattgt caaaaggctc tcgcggaaaa cggcgacaat atcgagaaag
     1621 ccatcgactt cctccgccaa aagggactcg cggccgccca aaagaaagcc ggccgagaaa
     1681 ccaaccaggg actcgtccac gcctacattc acatgggcgg gaaaatcgga gtcctcattg
     1741 aggtcaactg cgaaacggat ttcgtcgctc ggaatgaaga gttcaaggca ttcgtgaacg
     1801 acctggcgtt gcaggtcgcc gcggcaaagc cgtcgtatgt gaagcgagaa gagattccgg
     1861 gatccattat cgacaaagag cgaatgattt atgaaggaca ggccaaggaa atgggaaaac
     1921 cgccggcgac ctggcccaag atcatcgagg gaaagctgga aaaattctat caagagagct
     1981 gtctgatcga gcaggccttt atcaaagatc ctgccgtcac gatcaaagat ctgctatcac
     2041 agaagatcgc caagatcggc gagaacatca acatccgccg atttacgcgc taccaactag
     2101 gcgaagtatg agctctgcca aataccggcg catcctcctc aaagtcagcg gagagatgct
     2161 ggctggtgag cagggatacg gcatccaacc ctccgtcctc gaaggccttg ccgaggaaat
     2221 tgcgtccgtt gtagcgctcg atgtggaagt cgccgttgtc atcggtggtg gaaacatctt
     2281 ccgcggcatt gccgcgagcg cctcaggcat ggaacgagcc tccgccgact atatgggcat
     2341 gctggcaacg gtcctcaacg cactggcgtt gcaaaacgct cttgaacgca agggcgtcat
     2401 gacgcgtgtg caatctgcga ttgagatgcg tcagctggcc gaagggtata tccgccgccg
     2461 ggcgatccga catctggaga aaaaacgcgt tgtgatcttc gcggggggca ccggcaatcc
     2521 gtacttctcg accgataccg cggcggcgct ccgggccatg gagatcggtg cagaagtcat
     2581 catgaagggc acgaaggtcg acggcattta tgatgccgat ccggtgaaga atccgtcggc
     2641 caaaaagtac acggaaatct ccttcctctc gattctcaat caaaacctca aagtgatgga
     2701 tgccacggcc atcagccttt gtatggataa caatctccct ctaatcgtct ttgatctgaa
     2761 aaatcagggt aactttaagc gcgtgacgac cggcgagccc atcggcacga tcgtcaccgc
     2821 aaaaccccgc taaccctcgt cggagtcatt atgtcaaacc cagccgcgat ccgccagaaa
     2881 acgaccgaga aaatggaggc tgcgctcgag catctcaagc acgatttggc cggcttgcga
     2941 acaggacgtg catccgtcgc gctgttggat ggcgtgcggg tggattacta cggcaccatg
     3001 acgccgctca agcaggtcgc caatctctcg acgccggagg cccgactgat tacggtccag
     3061 ccgtgggagc cgcagctgat taaggaaatc gaaaaagcca tctcggcatc cgggctcggt
     3121 attactccat cgaacgatgg gaagatcatc cgcattcccc ttcccccgct caccgaagaa
     3181 cgtcggaagg agctgaccaa gatctgcaag aaacacgggg aagacaccaa ggtacatatc
     3241 cgcgggttca ggcgggaggc caacgaagaa ttgaagaaat tgcagaaaga tgcgaagctc
     3301 acagaggatg agc
//
LOCUS       DATDNW010000371         4758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1218189, whole genome shotgun sequence.
ACCESSION   DATDNW010000371 DATDNW010000000
VERSION     DATDNW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218189"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..421)
                     /locus_tag="VI359_08845"
     CDS             complement(5..421)
                     /locus_tag="VI359_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974398.1"
                     /translation="MCVGLSLMMVGGGVPADVRAADQEPMTVEEATRLGEEFGVIVGE
                     VDEEIQKELKLQRPQGVAVFEVIGNSRADYAGIKVRSVIKEIDKKEIRNMIDFGRSIK
                     VAMKECNFTLGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            complement(526..1347)
                     /locus_tag="VI359_08850"
     CDS             complement(526..1347)
                     /locus_tag="VI359_08850"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEY6974399.1"
                     /translation="MTTANRSRPLLLFFLSLMIVAGVLGGLGIPLVSSEGMIIRSQLV
                     DGELPTTPEDAAWAKVSPMTLPLSGQVITRPVWPEPTARALTVRSLHNGTEIAFLLEW
                     QDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRAA
                     RTGEKKSGEVRTFEVIPRRISSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGVWHVVM
                     RRPLSSEEQENEAKLIPGRIQTVSFAVWNGENKERNGQKAVAPWFQLVIDPVAKL"
     gene            complement(1449..2702)
                     /locus_tag="VI359_08855"
     CDS             complement(1449..2702)
                     /locus_tag="VI359_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974400.1"
                     /translation="METDVQLATGYIETFAGNGKARSTGDGKRAVKAGIPLPHHAALD
                     KEERWLYFAESGSDRIRRINLQEGTLHNFAGIGETCYSGDDGPCGEAGLYLPLDVAFD
                     SRNDLYLCDSGSNRIRKIDRETGIITTVVGTGQHGFNGDGPALEVNLTWPAAIAFDND
                     DVMYIADTQAHRIRRFDPRTGLVTTIAGGWTAEDDAREQPLVARNLVVLSGDAIGIDF
                     TDDHGWLMPVCSDGLDLSMYLDDGKPALEARLYDIVGIAVDSKGDVYVVDKGSNRVRK
                     IDIANGIISTVAGVCRYGYDGDDKPATKAMLHAPEAVVFDHQDNMYISDTMNHRVRRV
                     DAKAGVITTVAGNGDSGYEDKNMGGCGAARFVAKESAGTLKHGDGLLGVEAVVNSPVG
                     LALDSQGHLFVCERGENKIRRVKLW"
     gene            complement(2733..3014)
                     /gene="tatA"
                     /locus_tag="VI359_08860"
     CDS             complement(2733..3014)
                     /gene="tatA"
                     /locus_tag="VI359_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY6974401.1"
                     /translation="MFGSLGFTELILILVIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAAQQQMATAPQQAIPAAPAPAAPVVEQHAATAQPASRG"
     gene            complement(3037..3846)
                     /locus_tag="VI359_08865"
     CDS             complement(3037..3846)
                     /locus_tag="VI359_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6974402.1"
                     /translation="MNEHASTPHEPSPAAVDPGDMPLAPDEEIAELEKMLATEPDDFQ
                     ARCRLGELYFSKGRLDDALVEVKKSIEMAEGLRAEMNRSLAMYYSNLGTIYATKNMAD
                     EAQAEFKHALEVFPHDVLALFNLGRLHADKKQYLDAKNYYERLVEITPDDPIAWYNIA
                     GVYVELDNPQVSDYNTIDMAIQCYLRTLELDPKHLEASFKLMDLALNHKKTDLAIRVM
                     EDAVEQNPDEPLAYYNLINVYDKCKMFEQAEQTRQRLKDRFAKRAKESASS"
     gene            complement(3874..>4758)
                     /locus_tag="VI359_08870"
     CDS             complement(3874..>4758)
                     /locus_tag="VI359_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974403.1"
                     /translation="ILLFLGLGVAILNRAVGRSMNGFDKVTVPCIALLLLLTVGFLFV
                     PPTMLGSSIYNLVALVTLICLMTEYLSTHEEWSYRAMGLTYLFGIGGWLYYQIVSTAY
                     SFLGTVAAPPMVYEAHRAGEALMVLSSMLVFWAYGHGVSIRSRNQRQRRRAVWFWTTA
                     VSIFVGMLFLDYLLDHYNSTMAANIRSGARGIGWIFQFGMGYTFYLPFAFYMAGLICW
                     SYTVIKLLTMGRLAGYGIGLMFIAGYALLYSNLTLMVILGVMLLTMDRAKRESVESAP
                     ASSASMMGAPDSLVSGHV"
BASE COUNT          991 a         1464 c         1272 g         1031 t
ORIGIN      
        1 gttcctagtc tcgcttgcac ccgacgaagt gaaagttgac accccaccca accggatcgc
       61 ccgggtctgc aggctcataa gttcccaacg taaagttaca ttctttcatc gccactttga
      121 tcgatcgtcc gaaatcgatc atatttctga tttccttttt atcgatctcc ttaatgactg
      181 agcgcacttt aatccctgca taatcagccc gtgaattgcc gatcacttcg aagaccgcta
      241 cgccctgagg ccgctgcagc ttcaattctt tctggatttc ttcatcgact tcgccgacga
      301 tcacaccgaa ttcttcaccc agccgcgtgg cctcttcgac cgtcatgggc tcctgatcgg
      361 ccgctcggac gtcagcgggc acacctccgc cgaccatcat aagcgacagg ccaacacaca
      421 taaaaaaagc cctcctgcgg cgaagggctt gctgtcgtcg atccgtcaaa ccgatgtctc
      481 ctccaatgct gacgcaaacc ttcttgttac atcacaccaa gaactctaca gtttcgcaac
      541 cggatcgatc acgagctgaa accacggcgc caccgccttt tgtccgttcc gttccttgtt
      601 ctctccgttc cagaccgcga acgacaccgt ttggatgcgg ccggggatca gcttggcttc
      661 attttcctgc tcttcgcttg agagcggtcg ccgcatcacg acatgccaga ccccgtcttt
      721 ccacgtggcc ttgccctgca cccgcccctg cttctctttc gtcgtcagcg tgctgaaccc
      781 tccaccgatt aaatcttcca ccgacgagat ccgacgggga atgacttcga acgtgcgcac
      841 ttcgccactc ttcttttcgc cggttcgagc cgctctccga tcgatgtcgc tctgccagtc
      901 cgccttccaa tgccagatgt tgatgtagtg atcaagctgt cccatgcaaa agaacgccgg
      961 tgcatcgccg agcggaagcc cgattgcgac cccgtcccga aaagtcccgg gcgtcaaccg
     1021 gtcgttcttt gtgttgtctt gccattccag gaggaacgcg atctcagtgc cgttgtgcag
     1081 ggaccgtaca gtcagcgcgc gggcagtcgg ttccggccac accggcctgg taatgacctg
     1141 cccgctcaac ggaagggtca tcggagacac cttggcccag gccgcatctt ctggggtcgt
     1201 cggcaactcg ccgtcaacga gctgggaccg gatgatcata ccctcggaac tgaccaacgg
     1261 aatgccgagg cctcccaaca cgcctgccac gatcataagg gagagaaaga agagcaagag
     1321 gggacgggaa cggttagccg tcgtcatgcg caccttgcaa ggccctcgga tgagcagcgc
     1381 ctacgtcgcg agaggctcag cgtattacac accggggacg agaccagacg ggacgaccgc
     1441 cggcgacgct accagagttt cacgcgacgg attttatttt caccacgttc gcagacaaag
     1501 agatgtcctt gagagtccag agcgagcccg accggcgagt tcaccaccgc ctcgacgccg
     1561 agcaggccat cgccgtgctt cagcgtaccg gcggattctt tcgccacaaa tctggcggcc
     1621 ccgcagccgc ccatgttctt gtcttcatag ccgctgtcgc cgttacccgc gaccgtggtg
     1681 atgacaccgg ccttggcatc gacccgccgg acacggtggt tcatcgtatc ggagatatac
     1741 atgttgtcct ggtggtcgaa cacgacagct tccggcgcat ggagcatcgc cttcgtggcc
     1801 ggcttatcgt cgccgtcata gccataccga cagactccgg caactgtcga gatgatcccg
     1861 tttgcgatgt cgatcttgcg gactcggttg ctgcctttgt cgacgacata gacatcgcct
     1921 ttgctgtcga cggcaatacc cacaatgtcg tagagcctcg cctccaacgc cggcttgccg
     1981 tcgtccagat acatcgagag gtccagccca tccgagcaga ccggcatgag ccagccgtgg
     2041 tcgtcggtga aatcgatccc gatcgcatcg ccggacagga cgacgaggtt cctggccaca
     2101 agcggctgtt cgcgggcatc gtcctccgct gtccaaccgc cggcaatcgt cgtgaccagc
     2161 cccgtccgtg gatcgaagcg gcgaatccgg tgcgcctgcg tatcggcgat atacatcacg
     2221 tcgtcgttgt cgaatgcgat ggctgccggc cacgtcaggt tgacttcgag cgcgggaccg
     2281 tccccgttga atccatgttg cccggttccc accaccgtgg tgatgatgcc ggtctcacga
     2341 tcaattttac ggatgcgatt gctaccggaa tcacagaggt acagatcgtt gcgggaatcg
     2401 aacgcgacat caagcggaag gtacaggccg gcttctccgc aagggccgtc atccccgcta
     2461 tagcaggtct cgccgatgcc ggcgaaattg tgtaacgtgc cctcctgcag attgatgcgt
     2521 cggatacggt cggatccgga ctcggcaaag tagagccatc gctcctcttt atcgagcgcg
     2581 gcatgatgcg gcaagggaat accggccttg acggcgcgct tcccgtctcc ggtgctacgg
     2641 gcttttccgt tgcccgcaaa ggtttcgatg tatcccgtcg cgagttgaac gtccgtttcc
     2701 ataccttctg atcctcgtct gtggcccgga acctaccctc gcgaggccgg ttgggcggtc
     2761 gcggcatgct gctcgacaac gggagcggct ggcgccggcg cagcaggaat cgcctgctgg
     2821 ggcgcggtcg ccatctgctg ctgagccgcc tgggcttcgg cttcgatcgc atcggcttcg
     2881 tgcacagact tcttgaagcc cttgatggcc ttcccgatcc cttcacccaa ttgcggcaac
     2941 ttgcctgcgc cgaagataat cagcacgatg acgagaatca ggatcaattc tgtgaaacca
     3001 agactcccaa acatggtgta ttcctccgtg tgtaaatcag gatgatgcgc tttctttggc
     3061 ccgctttgca aatcgatctt tgagtcgctg cctcgtctgc tcagcctgtt cgaacatctt
     3121 gcacttgtcg tacacgttga tcaggttgta gtaggccagc ggctcgtctg gattttgttc
     3181 gaccgcgtct tccataaccc ggatggcgag gtccgtcttc ttatggttga gcgccaaatc
     3241 catcagcttg aaactggcct ccaaatgctt gggatccagt tcgagcgtac ggagataaca
     3301 ctgaatcgcc atatcaatgg tgttgtaatc cgaaacctgc gggttatcca gttcaacata
     3361 gacacctgcg atattgtacc aggcgatcgg atcgtccggt gtaatttcaa cgaggcgttc
     3421 gtaatagttc ttggcgtcca agtactgttt tttatccgca tggagtcgcc cgagattgaa
     3481 gagagcaagc acgtcgtgag gaaagacctc aagcgcgtgc ttgaattcgg cttgggcctc
     3541 atccgccatg tttttcgtgg cgtagatcgt ccccaaattc gaatagtaca tggcgagtga
     3601 ccgattcatt tccgcccgta gcccctccgc catctcgatg gatttcttga cttcgacgag
     3661 ggcgtcatcc agccgcccct tgctgaaata gagttctccg agtcggcagc gcgcctgaaa
     3721 atcgtccggt tcggtcgcca gcatcttttc gagttcggcg atttcttcat ccggcgccag
     3781 cggcatgtcg cccggatcga ctgctgcagg actcggttcg tgaggtgtcg acgcgtgttc
     3841 gttcatacgt gatcctattc caagacggtt acattataca tgcccgctca ccagtgagtc
     3901 gggagccccc atcatcgacg cgctcgaggc gggggcggat tccaccgact ctcgtttggc
     3961 gcgatccatc gtcaggagca tgacgcccag aatgaccatg agcgtcaagt tcgaatacaa
     4021 cagtgcataa cctgcgataa acatgagacc gatcccgtaa ccggccagac ggcccatggt
     4081 cagcagcttg atgaccgtat aggaccaaca aatcaatccg gccatataga acgcaaacgg
     4141 caagtagaag gtgtacccca tcccgaattg gaaaatccac ccaatgcccc tcgcgccgct
     4201 tctgatattg gctgccatcg ttgaattgta gtggtcgagg agataatcca agaacagcat
     4261 acccacaaag atcgacaccg cggtggtcca aaaccacacg gctcggcgcc gttggcgttg
     4321 attgcgcgag cgaatcgaca caccgtgtcc gtaggcccaa aacaccagca tgctggacag
     4381 gaccatgagg gcttcgccgg ctcgatgtgc ttcatagacc atgggcggcg cggcaaccgt
     4441 tccgagaaaa ctataggcgg tcgatacgat ctggtaatac aaccatcccc cgatcccgaa
     4501 caaatacgtg agccccatcg cacggtacga ccactcttcg tgtgtcgaca ggtactccgt
     4561 catgagacag atgagggtca ccaatgcaac caagttatag atggaggacc ccagcatcgt
     4621 cggtgggacg aagagaaatc ccactgtcaa caggagtaag agggcgatgc agggaacggt
     4681 aaccttgtcg aatccgttca tgctccgtcc gacggcgcgg ttgagaatcg caacgcccaa
     4741 tccaagaaag agcagaat
//
LOCUS       DATDNW010000372         3523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_527352, whole genome shotgun sequence.
ACCESSION   DATDNW010000372 DATDNW010000000
VERSION     DATDNW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527352"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..2303
                     /gene="glyS"
                     /locus_tag="VI359_08875"
     CDS             135..2303
                     /gene="glyS"
                     /locus_tag="VI359_08875"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY6974404.1"
                     /translation="MGRAKVATSKKYVTSRSSSAAELLVEIGSEELPFDFIAPALALL
                     KEQTERMLAESRLSYRHVHTVGTPRRLVVVVNGLTDHQAATTKETMGPSKAVAFDQTG
                     QPTKAAIGFASGQGLAVEDLHLRQTPKGDYLFAVKREEGQPAREVLTKLVPQIIAKLS
                     FPKSMKWNEAGVRFARPVRWLLALFGGAVLPIEIAGIKAGHNTYGHRVLGGGKAVVVR
                     DYSSYVSALKRVGVIVDQDKRRTLIHEELTQVSQKAGVHLNADEALLEQATYSTEWPV
                     PIVGSFRPEYLSVPSEILITSMKEHQGFFSVRDKKSGALAAHFIAVANNEVKDMSFIR
                     QGNERVLAARLADARFFYDEDRKTTLVERATKLSGVVFHQKLGTMAQKQDRITKLAGL
                     LAQQLGMSKESVEICSRAASLCKADLLSGIVGEFPELQGVMGGYYAQHDGEPIAVSHA
                     IMDHYKPRAMEGDLPRSLEGQVVSMADRIDTIAAFFHVGMVPTGSEDPFALRRHATAI
                     VRIVVEAGLRLDLGQAIEQARTIVAESGFKAVAGSDVQQERLVEFLFERVRYYGRVKH
                     GLRDDVMDAVLVVANHRRFDVQDQLAKMGALQGITTRQEFDPLIIGFKRAHRLVEKEQ
                     WDRGPVDPATFEDAAETELHRQNGLCRKDFEEHMRQGEYQKALDALVRVKPAIDAFFA
                     GVMVNAEDSTLRDNRLSLLKDVDDTFMSFADFSRIVVQGS"
     gene            2480..2995
                     /locus_tag="VI359_08880"
     CDS             2480..2995
                     /locus_tag="VI359_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974405.1"
                     /translation="MDQASPNEVVPRLSVEAEQRAGRVRRFGIAVSIALILICNGILL
                     FGVWASGVNLDELVRTPDYFNAKQDICLRLTWQKVAGASEPMRLCSEWINLADPSGKT
                     HQLQNDMKVRQGADGRYYVDQGVRADYRLLAVALFVVVVIVTGIVVKRYLVRRYQLHL
                     DTAAGQTSLVQ"
     gene            3021..>3523
                     /locus_tag="VI359_08885"
     CDS             3021..>3523
                     /locus_tag="VI359_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY6974406.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYKLGKKYPTGMWEASLQALKRVERSMGMGFGDPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAI
                     LKHKKTEV"
BASE COUNT          780 a          892 c         1106 g          745 t
ORIGIN      
        1 caatgtgcgg aacggtacgt cgaagaacgg gccaccatgg gtcacccact aaacgatcgt
       61 cctagcgcct cgtcgaaaac ggaacgccgt cagaccaccc gtcgcatatt caaatcgtag
      121 gaatgacctg atcgatgggc agagcgaagg ttgccacatc caagaagtac gtgacgtctc
      181 gctcatcgag cgcggccgaa ctgttagtgg agatcggcag cgaagaactc ccctttgact
      241 ttattgcccc ggccctcgca ttgctcaaag aacagaccga acggatgctg gcagaatcgc
      301 ggctgtccta tcggcatgtg cacacggtgg gtaccccccg gcgtctcgtc gtcgtagtga
      361 atggactgac agaccaccaa gctgccacga cgaaggaaac gatgggaccg tcgaaagccg
      421 ttgcctttga tcaaaccggt caaccgacga aggcggcgat cggatttgct tccgggcagg
      481 gtctagcggt ggaggatctt catctcaggc agaccccaaa aggcgactat ctctttgcag
      541 tcaaacgtga agagggacag ccggctcgtg aggtgttgac gaagttggtg cctcagatca
      601 ttgccaaact gtccttcccg aaatcgatga agtggaatga agcgggggtt cgattcgcca
      661 ggccggtgcg ctggttgctc gccctcttcg gtggggcggt gctgccgatc gagatcgccg
      721 ggatcaaagc tggccacaac acctacgggc accgagttct tggtgggggc aaagcagtgg
      781 tcgtccgtga ttactcctcg tatgtatccg cgctcaagcg ggtcggcgtg atcgtcgatc
      841 aagacaaacg gcgtacgttg attcacgaag agttaacgca ggtcagtcag aaggccgggg
      901 ttcaccttaa cgcggatgag gcgttgctgg aacaagcgac ctatagcaca gaatggccgg
      961 ttcccatcgt ggggagtttc agaccggaat atttgtcggt cccgtcggaa atcctgatta
     1021 cctcgatgaa ggaacatcag gggttctttt cggtgcggga taagaagagc ggtgccttag
     1081 ccgcacactt tattgccgtc gctaacaacg aagtgaagga tatgtcgttc attcgtcagg
     1141 ggaacgagcg ggtcttggcg gcgcgtcttg cggacgccag attcttctac gatgaggatc
     1201 gaaagaccac gctcgtggaa cgagcgacga aactgtccgg cgtcgtgttt caccagaagc
     1261 tcggtaccat ggctcagaag caggaccgaa taaccaagct cgccggcctg ttagcgcagc
     1321 aattgggtat gagcaaggaa tcagtagaga tctgttctcg ggctgcgagt ctgtgcaagg
     1381 ccgatctgtt atccggcatc gtgggggagt ttcccgagtt gcaaggtgtc atgggcggct
     1441 attatgccca acatgatgga gagccaatcg ctgtcagcca tgcgattatg gatcactata
     1501 aacctcgagc gatggaggga gatctgcctc ggtcgctcga agggcaagtg gtgtcgatgg
     1561 ccgaccgcat cgatacgatc gccgcgtttt ttcatgtcgg aatggtgccg accggatcag
     1621 aagatccatt tgctttgcgt cgccatgcga ccgccatcgt gcgcatcgtg gtcgaagcag
     1681 gcctccggct cgatctgggc caggccattg agcaagcccg gacgattgtt gccgagagcg
     1741 gattcaaagc ggtggctggg tcggatgtcc agcaagaacg gttggtggag tttctgttcg
     1801 aacgagtcag atactacggg agggtaaagc acggactgcg tgatgatgta atggatgccg
     1861 tgcttgttgt agcgaatcat cgccggttcg atgtgcagga tcagctggcg aagatggggg
     1921 cgcttcaagg aattacaaca cgacaggaat ttgacccgct tatcatcggg ttcaaacgtg
     1981 cgcatcggct tgtggaaaaa gagcaatggg accgtggacc ggtcgatccc gcaacctttg
     2041 aagatgccgc cgagacggag ttgcatcgac agaatggtct ttgtcgcaaa gacttcgagg
     2101 agcacatgcg acaaggggaa tatcagaagg cgttggatgc gttggttcgc gtaaaaccgg
     2161 cgatcgatgc ttttttcgcc ggcgtgatgg taaacgcaga ggactccaca ttgcgggata
     2221 atcgactctc attgctcaag gatgtcgatg acacctttat gtcattcgcc gacttctccc
     2281 ggattgtggt acaagggagt taggaaaact gagatggtct ggttagcccg cgtgctcgtg
     2341 caacgcgcgc cctcaggagg gcctcgttgg acgcgcgcaa gttgggacta agccagaccg
     2401 cctcttgatg agggatgaaa ggtcgggtag gaacggcggg ccttttgacc atcctgtgcc
     2461 tgtgaggggg acgcgcgcaa tggatcaggc ttccccgaac gaggttgtcc cgcggctttc
     2521 ggttgaagca gagcaacggg caggtcgtgt ccgccgcttc ggcattgcgg tatccattgc
     2581 cctgatcctc atctgtaatg ggattctctt gttcggcgtc tgggccagcg gggtcaatct
     2641 cgacgaatta gtcagaaccc cggattattt caatgcgaag caagacattt gtctcaggct
     2701 gacgtggcaa aaggtggccg gcgcgtcgga gccgatgcga ctctgttccg aatggatcaa
     2761 cctggctgat ccttcgggaa agacccatca acttcagaat gatatgaagg tacgccaagg
     2821 cgccgatgga cggtactacg tcgatcaagg agttcgggcg gactatcggt tgcttgcggt
     2881 tgcactcttt gtcgtggtcg tgatcgtcac ggggatcgtg gtgaaacggt atctggtccg
     2941 gcgctatcag ctgcatctgg atacggccgc tggccagacc tcactcgtac agtgacgtcc
     3001 tagcagaagg agaagggctc gtggcgaaga aatacgtcta ctacttcggc gacggcaagg
     3061 ccgaaggtac ctcgaacatg aaggaattgc tcggtggcaa gggcgccgga ctggcggaga
     3121 tgaccaacct tggcatctcg gttcctccgg gcttcaccat ttcaacggag gcctgcgttg
     3181 aatactacaa gctggggaaa aaatatccca ccggcatgtg ggaggcttcc ctgcaagcct
     3241 taaagcgtgt cgagcggtcg atgggcatgg ggttcggcga tccggaacga cccctactgg
     3301 tttcggtgcg ttcgggtgcg cgtgcgtcca tgccgggaat gatggacacc gtgctgaatg
     3361 tcgggttgac gacgaagacc gtcgaaggat tggcggcaaa aacgcggaat gatcggtttg
     3421 ctcaggacag ctaccgccgc tttgtctcaa tgttcggcag tatcgtcatg ggagtcccgc
     3481 gcgagcattt cgaggcgatc ctcaaacaca agaagaccga agt
//
LOCUS       DATDNW010000373         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_601704, whole genome shotgun sequence.
ACCESSION   DATDNW010000373 DATDNW010000000
VERSION     DATDNW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601704"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VI359_08890"
     CDS             complement(<1..183)
                     /locus_tag="VI359_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974407.1"
                     /translation="MTQGQTLIRVIDYDSGVEQYISNQGSQHARKLPAAGRTKSRSSE
                     SGWYRAHCLSQAGCGLI"
     gene            549..1247
                     /locus_tag="VI359_08895"
     CDS             549..1247
                     /locus_tag="VI359_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY6974408.1"
                     /translation="MSEPYTIPQAKSITLLNPKSPEPVTTQVVAGLERIGLAMKSRTW
                     RREGRSGLGPLQRQVLAVLQSQPKHVAQVSQIAEELAVRLPTASEAVATLERKRLVRR
                     RRSKSDGRVVTVELSAKGLRACSGNPHKPGALVTATATLSPREQASMLTALIKLIKSL
                     QDQGEISVARMCVSCRYFRPNQYDDPAKPHHCDYVNAPLGDMTLRVNCPEYESAPKGQ
                     ADAAWTAFSNSRIA"
     gene            complement(1539..1961)
                     /locus_tag="VI359_08900"
     CDS             complement(1539..1961)
                     /locus_tag="VI359_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974409.1"
                     /translation="MPFLALKGADHMPGIALLQWPAKDLRTAAISYIQQRRTFVSAGD
                     SPTDLTLTMKAWLTMLSRGEYRYILRLETDIGPADRPAVKSYVVQKEAVGSSVRWVTD
                     SDQDPIAQVVQAALDDLFTQIEADHALYRMSKNIGGKN"
     gene            complement(2108..2596)
                     /locus_tag="VI359_08905"
     CDS             complement(2108..2596)
                     /locus_tag="VI359_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974410.1"
                     /translation="MNRLRNLAVTMICLALGTLVYASCAHDSYQERANLIKDHAEAFY
                     GHLKANQVEAAIHDNEQIEAMASQMGETVRKRAAQQGTTQVEREFALMKTANEAAVTN
                     WLALGQYFSIKKQYAQARSTYQRIIDTYTNPSERPYREQAQRALRDVDILSPTTATSP
                     KP"
     gene            2759..2836
                     /locus_tag="VI359_08910"
     tRNA            2759..2836
                     /locus_tag="VI359_08910"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2795..2797,aa:Glu,seq:ttc)
     gene            2976..>3629
                     /locus_tag="VI359_08915"
     CDS             2976..>3629
                     /locus_tag="VI359_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY6974411.1"
                     /translation="MRGLSLLMWAGVMLISGISALHAAEPRVPQNGQSPAMETIKGQD
                     GAPMVLVPAGPFTMGSNDGLPNERPEHTVTLDGYYIDQYEVTLGLYRKFLEATKHEAP
                     PTWDDEAASTVGDRPAIGMKWADALAYCKWAGKRLPTEAEWEKAARGTDGRRYPWGQM
                     QPFVDIANYNRGVWVSETITLMGVTSGLEGMSVRHGLKEGGKSPYGLTHMAGNAAEWV
                     "
BASE COUNT          787 a          948 c         1059 g          835 t
ORIGIN      
        1 gattaaacca catccagcct gactcaggca atgagcccga taccaaccac tttcggacga
       61 gcgagatttc gtgcggcctg cagcgggcaa cttgcgggca tgctgcgatc cttgattgga
      121 aatgtactgc tccactccgg agtcgtagtc gattactcga atcagcgttt gtccttgagt
      181 catcggggcc actgcaaaac cgccatgcca tctgtagagt gtaatggcga gtccctgatt
      241 tctgacgacg ggtttggttg gggttttggt aacgatgaac aataagcatg agtcgcacgt
      301 cgtcgcagta gaaaggacaa tttggtgcaa gaattcaaag ggatcgtatc aagtgatctc
      361 cctcgatcat cctcagtggt aactgccgag taaatctttt aaagcgaggg cgctgggttc
      421 gagtcccagg cggctcatca cgtacttcag agttttcacc gaagagacat gcgccatact
      481 cttttcgaac cttgacggat gcgcgcggga tgatctagag tgaaacggtt gaatttggag
      541 agattcctat gtccgagccc tacacgatac ctcaggccaa atctattacg ctgttgaatc
      601 ctaaatctcc cgagcctgtc acgactcaag ttgtggccgg gttagagcga attgggcttg
      661 cgatgaaaag tcggacttgg cgacgagagg ggcgatcggg tcttgggcca ttacaacgac
      721 aagtcttggc tgtgttgcag tctcaaccga agcatgttgc ccaagtctcg cagattgcgg
      781 aggaattggc agtgagactc cccaccgcgt ccgaagccgt ggcgaccctt gaacgaaagc
      841 ggctcgttcg gcgacgtcgc tcgaagagcg atggacgtgt cgtgacggtc gaactctctg
      901 cgaaaggact gcgcgcctgc tcgggaaacc cacacaagcc tggtgcactc gtcaccgcta
      961 cggcaacgct gtcacctcgg gaacaagctt caatgctcac ggccttgatc aagcttatca
     1021 agtcccttca ggatcaagga gaaatttcgg tggcgcggat gtgtgtatcc tgccgctatt
     1081 tccggcccaa ccaatacgat gatcccgcca aaccgcatca ctgcgactac gtcaatgctc
     1141 cgctcggaga catgacgctc cgtgtgaatt gccccgaata cgagtcggcg ccgaaggggc
     1201 aggctgatgc cgcatggacg gctttttcca attctcgcat cgcctagagt cttttctcgt
     1261 gcacgaatgg tatctcgatc tcaggccctt cggtatgaag gttggatgat ggcggaacga
     1321 cacgctgcag gacggttcac gagacagagc gcaaacgtgc catcggagga atggttaggc
     1381 ggtgaatctc atgtgccggg tctgagtcgg gaattacgac agcggcatcg ttccacacat
     1441 cgatcgtctt ttcggttggt ggccttctca atattcatcg cagggttggc tcttggcacc
     1501 gtgatctccc tactgttgaa tccgcgtcgt ctttcgtcct aattcttccc ccctatgttc
     1561 ttgctcatcc gatacaaggc gtgatcagcc tcgatttgcg tgaagagatc gtcgagggcg
     1621 gcctggacga cctgagcaat tgggtcctga tccgagtccg ttacccatcg gacactcgac
     1681 cccacggctt ccttctgcac gacataggat ttgacggctg gccggtccgc cggaccaata
     1741 tccgtttcca agcggagaat ataccgatac tcacctcggg aaagcatggt gagccatgcc
     1801 ttcatggtca acgtcaagtc ggtcggggaa tctcctgcgg acacaaaggt tcgccgttgt
     1861 tgaatatagc taatcgcggc ggtgcgaagg tccttggcgg gccactgaag cagggcgatc
     1921 cccggcatgt gatcggcgcc cttcagcgcg aggaatggca cgataacttg aacggagtat
     1981 ggaatgggag tctccgcgac ggttggtggg ctcggtgcta catggatctt gtgggtgcag
     2041 ccgccattcc acaggctgag gcaacataag attgagccga tccacttgat tacaggggaa
     2101 ggtctagtca cggtttcggt gaggtggcgg tggtcggact gagaatgtcc acgtctcgca
     2161 atgcccgctg ggcttgttct cggtatggtc gttccgacgg atttgtgtag gtgtcgatga
     2221 tccgctgata ggtcgatctg gcttgcgcat actgtttctt gatcgagaag tattggccca
     2281 atgcgagcca gttggtcacc gctgcctcat tggcggtttt catcaaggcg aattcgcgct
     2341 ccacttgtgt cgttccctgc tgcgccgcac gcttgcggac ggtctcaccc atctgagagg
     2401 ccatcgcttc gatctgctca ttatcatgga tagccgcctc aacttggttg gctttgagat
     2461 gaccatagaa ggcttcggca tggtctttta tcaggttagc tcgttcctga tacgaatcgt
     2521 gggcacaact ggcatacaca agcgtaccca acgccagaca aatcatggtc acagccagat
     2581 tgcgcagcct attcatgcga attctcctct ggatttatgg ggtgcttcat tctttgagcc
     2641 ctcgttcatc ctaccctatt gcaggaggaa aggcgacggg gaggtaagga gcacggtcta
     2701 gtgacacatc agtcggaaat gtggtaagag tccctcctat cgcgtgaaac ttcgtcttgt
     2761 ccccatcgtc tagcctggcc taggacgccg gcctttcaag ccggcaacac gggttcgaat
     2821 cccgttgggg acaccaccgt ccataccgtt ccgtcacccg ttgagcctac gcgttccata
     2881 ctccgttgag tttgcatggg ggtctcggta aactagaagt attattttgg ctactactcg
     2941 tgtcggagag gcaggggacg accaaggagg ttgcaatgag gggactatcg ctgctcatgt
     3001 gggcgggggt gatgttgatc tcgggaatat ccgctctcca tgcggcggag ccacgggtcc
     3061 ctcaaaatgg acaaagtcct gcgatggaaa ctataaaggg ccaggatggc gcaccgatgg
     3121 tgttggttcc tgcagggccg tttacaatgg gaagcaacga tggattgccg aatgaacggc
     3181 cggaacacac cgtcacgctt gatggttatt acatcgacca gtatgaagtt actctcggac
     3241 tctatcggaa atttctggaa gcgaccaagc acgaagctcc gcccacatgg gatgacgagg
     3301 cagcctccac cgtcggtgac cgaccggcca tcgggatgaa gtgggccgat gccttagcct
     3361 attgtaagtg ggctgggaaa cgccttccca ctgaagccga atgggaaaaa gcggcgcggg
     3421 gaaccgatgg acgtcggtat ccgtgggggc agatgcaacc gtttgtggat atcgcgaatt
     3481 acaatcgggg agtctgggtg agcgaaacga ttacgttaat gggcgtgacc agcggacttg
     3541 agggaatgag cgttcgtcac gggctgaaag aaggggggaa aagcccctat ggtctcaccc
     3601 atatggcggg caatgcagcc gaatgggtc
//
LOCUS       DATDNW010000374         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1156845, whole genome shotgun sequence.
ACCESSION   DATDNW010000374 DATDNW010000000
VERSION     DATDNW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156845"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VI359_08920"
     CDS             <1..1405
                     /locus_tag="VI359_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEY6974412.1"
                     /translation="ELAPVLTGGWIQKIHQPAALTIVLEIRTPGQTYRLVISCFAETT
                     RLHLATASMQNPPSPPPFCQFLRAHLQGAHIDAISQVQDDRIVRIDLTTKAGPRALVA
                     ELTGKTANLFVLDSDDRLVRDLKRSTDRQGQPYRPPASPQHSLRVRSSIPLLHSMSAT
                     LFPLSAAIEATYQDRDAAALLDAARAAKIGVLKKSIKKLQRRIDAWHNDLTKAAKYRD
                     YGRYGELLKANLDMIKKGQREVAVVDYFEESMPALTIPLDSTKTPRWNMDDYFRKHRK
                     YVASERELRPRIEAGQAEVEAMCRELDTIKLGTWLPPPVFAAGDLERRDRPGSRTKLR
                     SQTRQGPFRRFTSADGLPIFVGRNAQENDDLTFRLANSDDLWLHARGTPGSHVVVRLA
                     KGQDVPPETLRDAATLALLYSDLKKSGKGDVIYTRRKWLKKAKGQAAGAVTVTQEQSL
                     FVTLDRIRLERLKTRSD"
     gene            1564..2064
                     /locus_tag="VI359_08925"
     CDS             1564..2064
                     /locus_tag="VI359_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY6974413.1"
                     /translation="MPKPTSTEQTILIVDDEPAVLGVVTTMLTQEGFRVLQANGSSDA
                     LRLCKNHQGRIDLLVTDLVLPPPGFSFASDANEFPHVHGHDLAVRALHMRKDLRIVLI
                     SGNMDKDLAGYGIRRGVLPFIEKPLQSPAFVNLVKQVLQGPPPTVESLTETHKNNPND
                     DNEWVD"
     gene            complement(2178..2510)
                     /locus_tag="VI359_08930"
     CDS             complement(2178..2510)
                     /locus_tag="VI359_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY6974414.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFGQHGGVASARIITDKFTGQSR
                     GFGFVEMSSDQEAQAAITALNGAEMGGRTLTVNEARPQEPRTGGGGGRGGFGGGGRSG
                     GGGKRDRW"
     gene            complement(2737..3528)
                     /locus_tag="VI359_08935"
     CDS             complement(2737..3528)
                     /locus_tag="VI359_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974415.1"
                     /translation="MKVHRIIALVSRYLYLYRRSLPRIMEIFYWPFLDLVIWGFITLY
                     LARFQNQVPGFVTFFLGALILWDILFRSQQGITISFLEEIWARNLMNLFASPLKPSEF
                     LAATMVMSIFKVTCVSIVMAVCAALFYSYNIFMIGLWLLPFVLNLVVTGWIVGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPLDVLPEWLKMIAWVNPAAHVFEGMRAVLS
                     TSSRPVVNLLWASGLNVLLLVVVVIWFYRTFAYCKEEGLLVRVGE"
     gene            complement(3958..>4342)
                     /locus_tag="VI359_08940"
     CDS             complement(3958..>4342)
                     /locus_tag="VI359_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY6974416.1"
                     /translation="DMVKKLEMEEFRSKITRKLSSGQMTRLTLAKAFLTQPKILFLDE
                     PTASLDPDIAHKIRHLLKEEQRASGLSILYTSHNMREMEAMSDRIIFLQRGKIVAEGT
                     ASEIVARFGQSDLEDVFLKLAREQS"
BASE COUNT         1072 a         1311 c         1092 g          867 t
ORIGIN      
        1 agaacttgcc cctgtgctca cgggggggtg gattcagaaa atccatcaac cagctgcgct
       61 cacaatcgtg ctggaaatcc gaacaccggg gcagacctat cgtctagtga tctcctgctt
      121 tgccgagaca acccgcttac atctggccac cgcatcgatg cagaacccgc cgtctcctcc
      181 tcccttctgc caattcctgc gcgcccatct ccagggcgca cacatcgacg ctatcagcca
      241 ggttcaggat gatcggatcg ttcgaatcga tctgactaca aaagcagggc cccgtgcact
      301 cgtggcggaa ctcaccggaa aaaccgcgaa tctgttcgtc cttgatagcg acgaccggct
      361 cgtgcgagac ctgaaacgat ccactgatcg gcagggacaa ccctatcgtc cgccggcctc
      421 tcctcagcac tcgctgcgcg tccgctcttc catacccctt ctccattcga tgtcggccac
      481 tcttttcccc ttgtccgctg ccattgaggc cacgtatcaa gatagggacg cagctgcgtt
      541 gctggacgcg gcacgagccg ccaagattgg agttctgaaa aaatctatca agaaacttca
      601 gcgtcgcatt gacgcctggc acaatgatct gaccaaagca gcgaagtacc gtgattatgg
      661 acgatatggc gagttgctca aggccaacct cgatatgatc aagaaagggc agcgcgaggt
      721 ggccgtcgtc gactacttcg aggaatcaat gcctgccctg accattccgc tcgattctac
      781 gaaaacgccg cgatggaata tggacgatta tttccggaag catcgaaagt acgtggcatc
      841 agaacgggag cttcgtccca ggatcgaggc gggacaagca gaggtcgagg ccatgtgccg
      901 cgagctcgac accatcaagc tgggaacctg gctgccacca cctgtattcg cagcaggaga
      961 cttagaaagg cgtgatcgcc cagggagtcg caccaagctg cgctcacaga ctcgccaggg
     1021 tccattccga cggtttacct cggcggatgg gctccccatt ttcgtcggaa gaaatgccca
     1081 ggagaatgac gacctcacat tcaggcttgc gaacagcgac gatctctggc ttcatgcgcg
     1141 cgggacgccg ggatcgcacg tggtcgtacg actcgccaag gggcaagacg ttcctccgga
     1201 aacgttacgc gacgcggcaa cgctcgccct actgtacagc gatctcaaga agagcggaaa
     1261 gggggacgtg atctatacca ggaggaaatg gctgaaaaaa gcaaagggcc aagcggccgg
     1321 ggccgtgacg gtaactcaag agcaatcgct gttcgtcacg cttgatcgaa tcagacttga
     1381 gaggctcaag acccgatccg actgattttc caatctcgtc atgctccttc cttcaaatcg
     1441 tataggattc gagctaccgc tcgccgcagg gacaattgcg aaaaccgcga tacttctagc
     1501 aaacctgacc ttgtttcatt accctagaat tattggacgg gaatacgtag taccggggcg
     1561 ctcatgccta aacctacttc tacggagcag accatactca ttgtcgacga cgaaccagcg
     1621 gtgctgggag ttgttacgac gatgcttaca caagaagggt ttcgcgtctt gcaagccaac
     1681 ggcagctcgg atgcgctccg actctgcaag aaccatcagg gaagaatcga tcttctggtg
     1741 accgatctgg ttttgcctcc acccggcttt tcgttcgcct ccgacgcaaa tgaatttcct
     1801 catgtccacg gccacgactt ggcggtccgc gcgctccaca tgcggaaaga cctccgcatc
     1861 gtcctgattt caggcaatat ggacaaggac ctagcaggtt atggcatccg ccggggcgtt
     1921 ctcccattca tagaaaagcc gctacaatct ccggcgtttg tcaatcttgt caaacaagtc
     1981 ctccaaggtc ccccacctac cgtagaatct ctcactgaaa cccacaaaaa taaccccaac
     2041 gatgacaacg agtgggttga ttagttcacg atcggaaagt caccggtttt tataaaccac
     2101 ctgttctgag cctcagaaac aaacgggggg ctgccacctt tcggtgacag ccccccatga
     2161 acgtgtattc gtgatgctta ccagcgatcg cgctttccgc cgccaccgct gcgaccgccg
     2221 ccaccgaagc cgccccggcc gcccccgccg ccggtacgcg gttcctgcgg acgcgcctca
     2281 ttgaccgtca aggtccggcc gcccatttct gcaccattca gcgcagtaat ggctgcctgc
     2341 gcttcctggt ccgaggacat ttccacgaag ccgaacccac gcgactgtcc cgtgaactta
     2401 tccgtgatga tccgcgccga ggcaacacca ccgtgttgcc cgaacagatc gctcaattgc
     2461 tgctcagtcg ccgaatacgg caacccaccg acataaatct tcgaacccat ctggggttcc
     2521 tcctttgagt atgatgatat tgtcttgcac tcgagggacg ggggaaggag cgggccgaag
     2581 acgcgaacta tgcggcgact taggtctgac ttccgacgaa attcccgggc taaggcaaca
     2641 tcgatattgc aggcctgcac ttgactgacc gccccaccgc caggccaatg cgataaagca
     2701 cctttaccct agcataggcc cgggttgatt acaaccttac tctccgactc gaaccaacaa
     2761 accttcttcc ttgcaatacg caaacgtgcg gtaaaaccat atgactacga cgacaaggag
     2821 caggacgttg agcccgctcg cccatagcaa gttcacgact gggcgacttg aagtactgag
     2881 caccgcccgc atgccctcaa acacatgggc tgcgggatta acccaggcga tcatcttcag
     2941 ccactcaggc aggacatcca gcggatagaa cacgcacgag atcggttgga acaggaacac
     3001 catgctccaa gccaacactt cagcctcttg accgaaccgc ataatcagtg acgttgtaaa
     3061 gacacccacg atccaaccgg tcacgaccaa attgagcaca aatgggagca gccacagacc
     3121 gatcataaag atattgtagg agtaaaagag cgcggcgcac accgccatga caatcgacac
     3181 gcacgtcact ttgaagatgc tcatgaccat ggtggcagca aggaattcgc tgggcttgag
     3241 tggactcgcg aaaagattca tgagatttct tgcccagatt tcttcgagga aggaaatcgt
     3301 aattccttgt tgggatcgaa acagaatgtc ccagaggatg agcgctccca gaaaaaacgt
     3361 cacgaacccc ggaacctgat tctgaaagcg ggcaagatag agcgtaatga acccccaaat
     3421 gacgaggtcg aggaagggcc aataaaagat ttccatgatc cgaggcaagc ttcggcgata
     3481 gagatagaga taacgtgaca ccagcgctat gatccgatga accttcatac tcgatcctcg
     3541 tgatggtgat gttcatcgcc gcagccaccg caatcatggt gtcggcccag gatcgtatcc
     3601 atcatggcct gtgttcctcc aaccagtcga cctgcgacac ggctcccgtc tggtaacatt
     3661 cgcattccga gtacagttcc cacgactggc aatatgaata taccgatcac gaaagggaga
     3721 gaggcgagac gtccccgatc gacggcccac agtaggacgc ccaatagcac tcccggcacc
     3781 agcgcggctg tgaggctcgc ccggaccacc ctccaatcac aggtatgccg tgcaaaacct
     3841 acaaatgcga aggcacacca aaacaaaccc cagcaggtac tcagtccgag tccgccgatc
     3901 accacgccaa gcagaactgt gaatacgtcg tccatacgat tcgacgaaac ccgcgtctta
     3961 cgattgctcc cgagccaact tcaagaacac gtcttccagg tctgactgac caaatctcgc
     4021 aacaatctcg gaagccgtcc cttccgccac aatcttgccc cgctgcaaaa agataatacg
     4081 atccgacatc gcttccatct cccgcatgtt gtgcgaggtg tacaggatgc tgagtcctga
     4141 cgcgcgctgc tcctccttga ggagatgtcg aatcttgtgt gcgatgtcgg ggtcgagact
     4201 agccgtcggc tcgtccaaaa acaagatttt cggctgtgtg agaaaggctt tcgccagcgt
     4261 gagtcttgtc atctgccctg atgaaagttt tctggtgatc tttgaacgaa attcctccat
     4321 ctccaatttt ttcaccatgt cg
//
LOCUS       DATDNW010000375         4642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_56897,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000375 DATDNW010000000
VERSION     DATDNW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56897"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VI359_08945"
     CDS             complement(<1..897)
                     /locus_tag="VI359_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974417.1"
                     /translation="MTARDLIHGWATKLANEQKQIATTGIDDPIPSVSGRLLQSVGTL
                     HLYELTLPRPRLMEIDTPITIIPPDEMEPTEGIVLRNRGPVLLVQTFDPIGQSFASAT
                     VIPDRAGFLTIAAQRLTDMTTQADAHRLGPADRLASLLESVSEDERHGGAAVGSSILT
                     TVWTDDVSLRHQRLASLAIELIKTNKRILVVSRDHDAADVLVGTIARAMKAAGLMYKT
                     WISRYEMALTQHAAGIAIHELGFEAQMHQFYAKARAEKAALRKKYNRFRELTPLLAYK
                     RQKQKDLDEVRLLEWRLLTQLSD"
     gene            1102..1575
                     /gene="rplI"
                     /locus_tag="VI359_08950"
     CDS             1102..1575
                     /gene="rplI"
                     /locus_tag="VI359_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY6974418.1"
                     /translation="MKVILQETMDGVGHLGDLVNVSDGYARNFLLPRNKAVEANSRNI
                     KAFEHIKRVAAERAKKEKLEIEAHAKKVSAVTLTVHAQVGKDDKMFGSVTAKDIAEGL
                     AEHGFTVDRRKIQLAQPIKELGTFTVPVKLPREVTATVTVRVVKKQDAETASAES"
     gene            1592..2854
                     /gene="glyA"
                     /locus_tag="VI359_08955"
     CDS             1592..2854
                     /gene="glyA"
                     /locus_tag="VI359_08955"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY6974419.1"
                     /translation="MNDAVGSLDALKATDLDVYAAIEAEEQRQREKLLLIASENFASP
                     AVLAAQGSLMTNKYAEGYPGKRYYGGCQHVDTVEELAIQRCKQIFGAEHVNVQPHSGS
                     QANMAAYLSVLKPGDTILGMDLAQGGHLTHGSKVNFSGMIFRAFSYGVDRQTEIIDYA
                     AVRKLAEDCRPRMIVVGASAYARTLDFPKFQEIAKSVGAYLMVDIAHIAGLIAAGFHP
                     SPIPYADFVTTTTHKTLRGPRGGVVMCKAEHAKAVDKFIFPGMQGGPLMHVIAAKAVA
                     FKEALSPGFKRYQQQVIANARTLAQGLLERGYKIVSGGTDTHLMLVNLTNKGITGKEA
                     DAALDAAGIIVNKNAVPYDEKPPAVASGIRLGTPIVSTRGMRESEMKEIVALIDRVLQ
                     HRQDLAVLDEVRSQAKALCGRFPIFHTY"
     gene            2882..3346
                     /gene="nrdR"
                     /locus_tag="VI359_08960"
     CDS             2882..3346
                     /gene="nrdR"
                     /locus_tag="VI359_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY6974420.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGEVIRRRRECLGCKRRYTTYERV
                     EEILPMVVKKDGRRETFDRTKILAGLKKACEKRPISIATIETVTDRIEKRIQEMGETE
                     IESRVIGEELMKELHQLDQVAYVRFASVYREFKDIDQFMDELRSLAQQRRER"
     gene            3452..4561
                     /locus_tag="VI359_08965"
     CDS             3452..4561
                     /locus_tag="VI359_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY6974421.1"
                     /translation="MPTTRPKLARARQMTNTPGITHVGIIGAGRGGTALMEIFANDPL
                     VEIVGVAEINPKAPGLSLAKRLKIPVTKDYRELLDLEQMDLLIDVTGNPDVGQFLQDF
                     HRMGVTVIGGASAKFMWQLIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEERTR
                     IACEIHDGLVQSLAGVNFKLDLCQQLLRKDPRASLATLRESKAQLKLAIQEARQVIFN
                     LRPLQYDKMELIPALTNYLKSYETQYHIRTAFRVSGDEQILFPRTKIFLFRIVQEALS
                     NVQKHAKADRVSVELDIALEHLRVTIRDTGVGFDMETVLRDPEKWDHFGIRGIIERAR
                     LVGGDGRIESKKGRGTTIVVDVPLVNKEAAHNGKN"
BASE COUNT         1078 a         1261 c         1356 g          947 t
ORIGIN      
        1 atcactgagc tgcgtcagca gccgccactc taagagacgg acttcgtcca aatccttctg
       61 cttctgcctc ttataagcca acaacggggt gagctcccga aagcgattgt acttcttcct
      121 cagggcggct ttctccgccc gggccttcgc atagaattga tgcatctgcg cttcgaatcc
      181 gagttcgtga atggcgatac cggctgcatg ttgggtcaaa gccatctcat aacggctgat
      241 ccacgttttg tacatcaagc ccgccgcctt catggcgcga gcaatcgtgc cgaccaagac
      301 atccgcagca tcatgatcgc gactgaccac gaggattcgc ttattcgtct tgatcaattc
      361 aatcgccagt gaagcgagcc gttgatggcg cagcgagaca tcatccgtcc acacggtagt
      421 caggattgag gatccaacgg ctgctccgcc gtgacgttca tcttcagaaa ccgattcgag
      481 aagcgaggcc aaccggtccg cgggaccgag gcgatgggca tcggcctggg tcgtcatgtc
      541 ggtcagtcgc tgggctgcga tggtcaaaaa cccagctcga tcaggaataa ccgtggcaga
      601 tgcgaacgac tgtccgatgg ggtcaaacgt ttgcactaac aaaactggac ctctattgcg
      661 gagcacaatc ccctctgtgg gctccatctc gtcaggaggg atgatagtaa taggggtatc
      721 gatctccatg agccgtggcc gagggagtgt caattcatag agatggagcg tgccgacgct
      781 ttgcaagagt cgaccggaca ccgagggaat aggatcgtcg atcccggtcg tcgcaatctg
      841 cttctgttca ttggccagtt ttgtggccca cccatggatg agatctcgtg cggtcatgca
      901 gatataacgt ggtgttgtcc gtgagtggat gccatcatac gagcggctga gcaaaactgt
      961 ccagcacctg cagcagggtc tggggattgg ccaaggggaa atccgcactc acgatcaggc
     1021 gcgggtgaac gagaatgcga attgactttt cgccgccccc ccagtatcct cttttccttt
     1081 ccaacagaag gagttagggg tatgaaggtc atcttgcagg aaaccatgga tggggtcggc
     1141 catctcggcg atctggtgaa cgtctctgat ggctatgcac gaaacttttt gcttcccaga
     1201 aacaaggccg ttgaggccaa tagccggaac atcaaggctt tcgagcacat caagcgagtg
     1261 gcggcggagc gggccaagaa agaaaaactt gaaattgaag cgcatgccaa gaaggtgtct
     1321 gccgtaaccc tcaccgtgca cgcgcaagta ggcaaggacg acaaaatgtt tggctccgtg
     1381 acggcgaagg acattgcgga aggactcgcc gagcacggtt ttaccgtgga tcgacggaag
     1441 atccagttgg ctcaaccgat caaggaactt ggcaccttta cggtcccggt caaactgccg
     1501 cgagaggtga ctgcaactgt gaccgtgcgc gtggtcaaga aacaagacgc ggagacggca
     1561 tctgccgaga gttgagcgag gaagacctgt gatgaatgat gcagttggtt cgttagacgc
     1621 cctcaaagcg acagatctgg atgtctatgc ggccatcgag gccgaggaac aacggcagcg
     1681 ggaaaagctg ctcttgatcg cctcggagaa ctttgccagc cctgctgttc tcgcggcgca
     1741 agggtccttg atgacgaaca agtacgcaga agggtatccg ggcaagcgct actacggcgg
     1801 atgtcaacac gtcgacacgg tcgaggagct agccatccag cgttgcaaac agatctttgg
     1861 cgccgaacat gtgaacgtcc agccacactc tggctcacag gccaacatgg cagcctacct
     1921 ctccgtcctc aaacccggag acacgattct cgggatggat ttggcgcaag gcggccattt
     1981 gacccatggc agcaaggtca atttttccgg catgatcttc cgggcctttt cgtacggggt
     2041 ggatcgccag accgaaatca tcgattacgc cgcggtgcgg aagctcgctg aagactgccg
     2101 ccccagaatg atcgtggtcg gggcaagtgc ctatgcccgc acgctcgact ttccaaagtt
     2161 tcaagagatc gccaagtcgg tgggcgccta cctcatggtc gatattgccc atatcgcggg
     2221 cttgatcgcg gccgggtttc accccagccc gatcccctac gcagactttg ttacgacgac
     2281 cactcacaag acgctccgtg gaccccgcgg gggggtagtg atgtgcaagg cggagcatgc
     2341 gaaggccgtc gataagttta ttttccccgg gatgcagggc gggcccttga tgcatgtgat
     2401 cgccgccaag gctgttgcgt tcaaggaggc cctctctccg ggattcaagc ggtatcagca
     2461 gcaagtcatt gcgaacgccc gcacgttggc tcaaggcctg ctcgagcggg gatacaagat
     2521 tgtgtcgggg gggacggaca cccacctgat gttggtgaac ctgacaaaca agggaatcac
     2581 tggcaaggaa gcggatgcgg ccttggatgc ggccggcatc atcgtcaaca agaacgcggt
     2641 gccttacgac gaaaagccgc ctgccgtcgc cagcggcatc cggttgggca ccccgatcgt
     2701 ctcgactcgt ggcatgcgcg aatccgagat gaaggaaatc gtggcattga ttgaccgcgt
     2761 cctgcagcac cgacaagatc ttgcagtcct cgatgaagtc cgcagccaag ccaaggccct
     2821 gtgtggacgg tttcctatct ttcacaccta ttgatcgtcc gataaggtag agtcgccgcc
     2881 tgtgaaatgt ccgttctgcg atgagctcga agacaaggtg gtggactcgc gtatggcgaa
     2941 agagggcgag gtcattcgtc gtcgccgcga gtgtcttggt tgcaagcgcc gttacaccac
     3001 ctacgaacga gtcgaggaaa ttctcccgat ggtcgtgaag aaagacggtc ggcgagagac
     3061 tttcgatcgc accaagatcc tggccgggct caagaaggcg tgcgagaaac gccccatcag
     3121 catcgccacg atcgaaaccg tgacggaccg gatcgagaaa cgtatccagg agatgggtga
     3181 aacggagatc gagagtcggg tcatcggcga agaattgatg aaggagcttc atcaactgga
     3241 tcaggtcgca tatgtccgat ttgcctctgt ctatcgtgaa ttcaaagaca tcgaccagtt
     3301 catggacgag ctgagatcgt tggcccagca gcgccgagaa cggtgacgcc gccgcgctcg
     3361 tttatcctct cttcgccttc gcgtgttcat cctcgattga cctcttgttg ccggtcatgt
     3421 atccgcatgc cttaaatggg taaaggctga gatgccaacg acgcgtccaa aacttgctcg
     3481 agcacggcaa atgaccaaca cgccgggaat cacccatgtg ggcatcatcg gcgcggggcg
     3541 tggcggtact gcgctcatgg agatttttgc caacgatccc ttggtggaga tcgttggcgt
     3601 ggccgagatc aatccgaagg cccccgggct tagcttggcc aagcgtttga aaattcccgt
     3661 cacgaaagat tatcgcgaac tcctggattt ggagcagatg gatttgctca tcgacgttac
     3721 gggaaatccc gacgtggggc agttcttaca ggactttcac cgcatgggtg tgacggtgat
     3781 cgggggagct agcgccaagt tcatgtggca gctgatcgag gcccgcatcc gggctaccgc
     3841 ggagatcgag aaaactctca ataagtacca gtcgctctac cgcctctacg tcaaggaaac
     3901 tggagccgca gtgaccgaag agcgtacccg catcgcttgt gaaatacacg atgggttggt
     3961 tcaaagcctc gcgggagtga atttcaagct ggatctttgc cagcagctct tacggaagga
     4021 tccccgggct agcctggcga cgctccggga gtccaaggcc caactcaaac tcgccattca
     4081 ggaagcccgc caagtcattt tcaacttgcg tccccttcaa tatgacaaaa tggaactgat
     4141 tcccgcgtta acgaactatc tcaagtccta tgaaacgcaa tatcatattc gcacggcctt
     4201 ccgcgtttcc ggtgatgagc agattctgtt tccaaggacc aagatctttc tctttcgaat
     4261 tgtccaagag gccctcagca atgtacaaaa gcatgcgaaa gccgaccgcg tgtcagtcga
     4321 actcgatatc gcgctcgaac acctgagagt cacgatccgt gacacgggcg tcgggttcga
     4381 catggagacc gtgctgcggg atcccgagaa gtgggaccac ttcggcattc gcggcatcat
     4441 cgagcgggcc cggctggtcg gcggtgatgg aagaatcgaa tcgaagaaag gacgcggcac
     4501 gacgattgtg gtcgatgtcc cattggtgaa caaggaggcg gcacacaatg gaaaaaatta
     4561 aggtactaat cgccgacgac catcgcgtcg tgagagaagg gctggcagct atcctcaaat
     4621 caaaggacga catcaatgtg gt
//
LOCUS       DATDNW010000376         4492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_399369, whole genome shotgun sequence.
ACCESSION   DATDNW010000376 DATDNW010000000
VERSION     DATDNW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399369"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VI359_08970"
     CDS             complement(<1..367)
                     /locus_tag="VI359_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY6974422.1"
                     /translation="MTKLSPEEVKAWGESFEAKQPQEVLAAAIEQYAPEIVLACSFGA
                     EDVVLVDMIHRINPTVPLFYLDTDFLFPETYGVRDRVIERYGLKPEQIIQMKSLLTPE
                     AQAQQYGERLWAQEPREKGP"
     gene            complement(531..3026)
                     /locus_tag="VI359_08975"
     CDS             complement(531..3026)
                     /locus_tag="VI359_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY6974423.1"
                     /translation="MSRTLTSTISIQTQPIPTAIAEEIETFEAEANRMIAGEIANDLF
                     KPFRLQYGIYGQRQAGVQMVRIKIPFGGLTSNQLRRVAELAEQYATGVGHVTTRQDIQ
                     LHFVELKNVPTMMRGLAEVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKTVA
                     LHLLRNPLNQSLPRKFKIAFSGCKHDCALTPIHDIGLLALRREDGTIGFRMVAGGGLG
                     SAPRIAQVLREFTPIDELIPSIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFAEFKRR
                     WEQAYTDMGYARPNHEPIKLLEYADQPAPLIMPTSNGTKRAAVSTNGHPNGQRQETPF
                     EMWKRTNVVRQKQEGYATAAIKLFMGDLTSDQMLFVADLADRYSNGNLRTTINQNMVI
                     RWVSEERLAEFYQDLLSQNLADPGAELVEDIIACPGTDTCGLGITSSKGMARALAEVF
                     PPGRVPEDLAGVSVKISGCHNSCAQHHIATIGLHGVGKRLGDHVAPHYELHLGGKVNG
                     TAKIGQMTVKLPAKSVPAAITHLIEVYRRDRQPGEGLPAYIDRMGKHKLKDELIPYTI
                     VPSHDEDPTFYYDWEGDNEFVLEDLGPGECAGGALEMIENGILEADQELYQAKLLVEK
                     HQYSVSVNKSYRAVLAAAKALLVTEGIDPATDADTLREFEQRLMPKGVVPSTYRDLGK
                     LIGDLGPKETTAALAQSKMAFAKGFVDACRVATERIGKDLKLAGEGEASPVPKPVVLS
                     VKDIAAVVSAPTSNGAPVYDLRGVACPLNYVKTKLKLEMMDAGEQLEIWLDAGEPIKN
                     VPMSLKNDGHKVLLQEALEPEAAHYRVLVEKVE"
     gene            complement(3037..3486)
                     /locus_tag="VI359_08980"
     CDS             complement(3037..3486)
                     /locus_tag="VI359_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY6974424.1"
                     /translation="MKVSLRATYGIMAAVDLAMGTGVTPIQARSIARRQAIPARFLEQ
                     VLHAMKKAGLVMSLRGAQGGYVLTKKPADLSVAEILEALDGPLLPSTGMNGQSPSRRP
                     NKPELLLGKVWEQVHQAERQVLEAITVEELADQQRTLEQQRSLMYHI"
     gene            3911..>4492
                     /locus_tag="VI359_08985"
     CDS             3911..>4492
                     /locus_tag="VI359_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY6974425.1"
                     /translation="MSLSSSVFSVLLLTSDPEIQAQYKQVFKDASITMVKDLAAIPKD
                     ATRRTFDAVIVESKPGSSPGAYALPSNMDLSHCLVITGSRSALKRASKFMQVMGKHKT
                     PTVNGAGALSLEDYLEGKMGDFVKGMRNGSARNLHPMLITAMERPLITLALQETKGNQ
                     IQAAELLGLNRNTLRKKIHDLHIPVKRGRTVQSR"
BASE COUNT          947 a         1278 c         1185 g         1082 t
ORIGIN      
        1 ccggcccttt ttctcgaggc tcttgagccc acaagcgctc accatattgt tgcgcctggg
       61 cttccggagt gaggagcgac ttcatctgaa tgatctgttc cggcttcaat ccataccgct
      121 caatgactcg gtcacggact ccgtaggttt cgggaaacag aaaatccgta tccagataaa
      181 acagtgggac ggtcggattg atacgatgga tcatgtccac caggacgacg tcctctgctc
      241 cgaaactgca ggccagcacg atctcaggcg catattgttc gattgccgcc gccaacacct
      301 cctgcggctg cttcgcttcg aacgactctc cccaggcctt cacttcttct ggcgacaact
      361 tcgtcatctg atcccttctt ccatctacca caacgcgcgg catagggata atgcccgagt
      421 gcgcacgtca ttctcacccg cccgccccag acgcaaaacg cgccttgacc cgagcgaggg
      481 ccttccgagg ccgggccttg ctcgagcaag ggcattatcc ctatgccgca tcactctact
      541 ttttcgacca atacccgata atgtgccgct tccggctcga gggcctcttg caggaggacc
      601 ttgtgcccgt cattttttaa gctcatcggg acattcttaa tcggctcccc tgcgtcgagc
      661 cagatttcca gttgctcacc ggcgtccatc atctcgagtt tcaacttcgt cttcacatag
      721 ttcagcgggc aggctacgcc gcgaagatcg taaaccggcg caccgtttga tgtaggggca
      781 gacaccaccg ctgcaatgtc cttcactgac agcacaaccg gcttggggac aggggacgct
      841 tcgccctctc ctgcgagctt caaatctttt cctatccgct ctgtcgcaac acggcaggca
      901 tcaacgaagc ccttggcaaa tgccattttc gattgagcca gcgctgcggt ggtctctttc
      961 ggtcccaagt ctcctatgag tttcccaagg tcgcgatatg tggaaggaac aacgcccttg
     1021 ggcatcaggc gttgttcaaa ttcccgtaag gtgtccgcat cggtcgctgg atcgatccct
     1081 tccgttacca acaaggcctt cgccgcggcc aggactgctc gataagactt attcacggaa
     1141 actgagtact gatgtttctc aaccaaaagt tttgcctgat acagttcctg atccgcttcg
     1201 agaatgccat tctcaatcat ctcgagcgct ccgccagcac attcgcccgg cccaaggtct
     1261 tcaagcacga attcgttgtc cccttcccag tcatagtaga aggttgggtc ctcgtcgtgt
     1321 gacgggacaa tggtataagg gatcagctcg tccttcagtt tgtgcttccc catccgatcg
     1381 atataggccg gcaacccctc tcccggctgg cgatcccgtc gatagacctc aatcagatgg
     1441 gtgattgcag ccggaacgct cttcgccggc aacttcacag tcatttgacc aatcttggcc
     1501 gtcccgttca ctttcccgcc aaggtgcaac tcataatggg gtgccacgtg gtcaccgaga
     1561 cgcttcccca cgccgtgtaa cccgattgtg gcgatatgat gttgcgcgca ggaattgtgg
     1621 cagccgctga ttttgacgga cacgccggca agatcctccg gcactcgtcc gggcggaaag
     1681 acttcggcaa gcgcacgagc catgcccttt gaggacgtga taccgagtcc gcaggtatca
     1741 gtccccggac aggcgatgat gtcttctacg agttccgccc cagggtccgc aagattctgc
     1801 gacaggaggt cctggtagaa ctcggccagc ctctcttccg aaacccaccg aatgaccata
     1861 ttttgattga tcgtcgtccg gaggttccca ttggaatacc ggtctgccag atccgcaaca
     1921 aagagcatct ggtccgacgt gaggtcgccc atgaacagct taatcgccgc agttgcgtac
     1981 ccctcctgtt tttgtctaac gacgttggtc cgcttccaca tctcaaacgg cgtctcttgt
     2041 ctctgtccat tggggtggcc gttggtggag acagcggcgc gcttcgtccc attggacgtt
     2101 ggcatgatga gtggcgcagg ctgatcggcg tactccaaca gtttaatagg ctcatgattc
     2161 gggcgagcgt agcccatgtc cgtatatgct tgttcccaac ggcgcttgaa ttctgcaaag
     2221 cctagtttct cgatcacgaa tttcattcgt gctttattgc ggttctttcg attacctaac
     2281 gtatcgaaga ccttgatcac cgcttcgatg ctcggaatga gttcatcgat cggcgtaaac
     2341 tcccggagca cttgggcaat gcgaggagca gaccctaacc cgccgccggc aaccatgcgg
     2401 aacccgattg tgccatcttc tcgtcgaaga gccagtagcc cgatgtcgtg gataggcgtc
     2461 agagcgcagt cgtgtttgca gccggaaaat gcgatcttga acttgcgcgg caagctttgg
     2521 ttcagcggat tgcggagcaa gtgcagcgcg accgtcttgg cgtagggcgt gacatcaaag
     2581 acctctccct gacagacgcc ggcaagatga catgcggtga cattgcgcac ggtgttcgcg
     2641 caggcttctc tcgtggtgag ccccacttcg gcgagaccgc gcatcatcgt cgggacattc
     2701 ttcagttcga cgaaatgcag ttggatatcc tgtcgggtgg tcacatggcc gacaccggtg
     2761 gcgtactgtt ccgccagttc ggccacccgg cgaagctggt tggaggtcag gcccccgaaa
     2821 ggaatcttga tacggaccat ttgcacacct gcctggcgct ggccatagat gccatactgc
     2881 aggcggaacg gcttgaacag atcgttcgcg atttctcctg ctatcatccg gtttgcttca
     2941 gcttcaaatg tttcaatctc ctcggcaatc gccgtcggaa tcggctgggt ctggatagaa
     3001 atagtactcg tcagtgtgcg gctcatggga atcacctcaa atgtgataca tcaaggatcg
     3061 ttgttgttcg agggtacgtt gttggtcggc aagttcttcg accgtgattg cctccagcac
     3121 ttggcgctct gcttgatgga cctgctccca gactttaccc agcaggagtt cgggtttatt
     3181 tggccgcctt gaaggtgact ggccgttcat gcctgtcgac ggtaggaggg ggccatcgag
     3241 agcttcgaga atttcggcca ccgacaggtc ggctggtttt ttggtcagca catatccacc
     3301 ctgagcccca cgaaggctca tgacaagacc agctttcttc atcgcgtgca acacctgctc
     3361 aagaaagcgt gcgggaatgg cttgtcgacg tgcgatcgac ctggcttgga tcggcgttac
     3421 acctgtaccc atggcaagat caacagctgc cataattcca taggtggccc ttagagatac
     3481 cttcatacct tagtattccg gtcggaatta gaattgtgtt attcatagcc tgctttcccc
     3541 tcatctgtca ataggcctct tggaggtgtg cggggagcat ttcgacggag attggaatcg
     3601 ttcctgtgct cattcatagg cgcgctcaag tcgaatggaa caggaatttc ggacggcaaa
     3661 tagacttatc gatccatttc aaccgcgaga ccagagtcca tacccctacg agatgacggt
     3721 ctgggtatgg aaacatcgct tgagcaagtt aacattcacc atcggtacag atgaatcatt
     3781 tttgttgagt gacattgatc ggcgtagaca gctgtgtgtt ttgtattgca tgtcctgctc
     3841 gtaaatttcc tgattgacat caaatcggct ctgggcaata atccggccct cttcttcacg
     3901 tggtgcgttc atgtcgctct cttcatccgt gttcagtgtc ttgctgctga caagcgatcc
     3961 agaaattcaa gcccagtaca aacaagtgtt caaggatgcg tcgatcacga tggtgaaaga
     4021 tctggctgcc ataccgaagg acgcgacaag acgaactttc gatgccgtga ttgtcgagtc
     4081 aaagcctggt tcatcgcctg gcgcgtatgc gctccccagc aatatggact tgtctcattg
     4141 tctggtgatc accggatcac gcagcgcgct caagcgcgca tcgaaattca tgcaggtgat
     4201 ggggaaacat aagactccga cagtgaacgg ggcgggcgcc ctgtcgcttg aggactatct
     4261 tgaagggaag atgggagatt tcgtcaaggg gatgcgaaac ggatcagcgc gaaatcttca
     4321 tccgatgttg atcacggcga tggagcgtcc tctcattacg ctggcgctcc aggaaaccaa
     4381 gggcaatcag attcaggcgg cggaactact cggcctgaat cgcaatacgt tgcgaaaaaa
     4441 gattcatgat ctccatattc ctgtgaaacg cggtcgaacc gtacaaagtc gc
//
LOCUS       DATDNW010000377         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1220890, whole genome shotgun sequence.
ACCESSION   DATDNW010000377 DATDNW010000000
VERSION     DATDNW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220890"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            646..1425
                     /gene="scpB"
                     /locus_tag="VI359_08990"
     CDS             646..1425
                     /gene="scpB"
                     /locus_tag="VI359_08990"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HEY6974426.1"
                     /translation="MRHLETRIEGEVGTITIDHPAKRNALSRQLVDAIVEALDRLSQE
                     RVRAIVLRAKPGVKVWSSGHDVEELPEGGRDPLGWDDPLRYLVRAIEEHPAPIITLIE
                     GGVWGGACEVVFACDLIVAAPSATFAVTPARLGVPYNVSGMLTFLNACSLRIVKEMAF
                     TAKPVSAERAERLAIINHMVAVERLESFVYELAQDIAENAPLAVSVMKEQLRMLAGAH
                     PMSPRRFERVQGLRRIVYDSEDYAEGIRAFKEKRKPLFSGR"
     gene            complement(1551..2348)
                     /locus_tag="VI359_08995"
     CDS             complement(1551..2348)
                     /locus_tag="VI359_08995"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEY6974427.1"
                     /translation="MTPSPEQEGKAPQEPTATWTDKEIEAALMQCIQALAPVTADVAP
                     LDPIRDGNCGAPAPVLVKSIGGGEKVSFDPPLVLDCAMVVGLDRWLKDSVQPAARKTF
                     SSPVSKIIGSSYACRNVYNLQDGHLSQHAFANAIDLPMFVLADGRKVDVTHGWGPTQR
                     DLIAAAKAKKATAGVTGSLAKQKTDAKGAATQVVNVSTSPASNSTTVASPSPTSADDS
                     EVAAKFLRLAHDGGCKIFSTVLGPEANDTHRTHLHLDLQERKTSVCE"
     gene            complement(3082..>3590)
                     /locus_tag="VI359_09000"
     CDS             complement(3082..>3590)
                     /locus_tag="VI359_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6974428.1"
                     /translation="SAKQKARGSGALAVSRDPTEYRRLYRAVGEDWLWFSRLLLTDAE
                     LAVIIGSADVEVYKLTDDGDGAGLLELDFREKNECELAFFGVSRTLIGGPAGRWLMNR
                     AIERAWSRPIRRFWAHTCTLDHSSALGFYKRSGFRPFRRQVEIAEDPRLSGLAPMDAA
                     AHVPLLAP"
BASE COUNT          755 a         1062 c          984 g          789 t
ORIGIN      
        1 cagccgatca aggcgaggac cacgactgtg ccgatgccga tgccagcacg accggcgggc
       61 atgccgcgac gatcttcaac gcgatcgctt ttcggtaggt catcgagacg catgacgcac
      121 cccacaacaa agtcggcgag catggaactg aactagcatg cagcaattag gcagatttgg
      181 cgcgttcgtc gttctgctcc tcgataatcc acccaacccg acgatgatgt cgagccaaaa
      241 accgtggtcc tcgacaatcc gaagatcagg caatttcgca agcgacttat cgtcagctat
      301 cctactccaa tagggcttac gtagctcgca gcgtcctcgc caagacgtct cgcgcccaga
      361 ttgtgcacct gcccgttagg agcaacccaa tgaaatacga tgatgcgctg ttcctgctcc
      421 tcataactgt gtttgcgatt ataggagtag gattgctttt agccttcctt ttttagcggg
      481 ccccagtggc agcccacagc atgcgcgtct gaggtcatga ccaaaccccc taaggagttc
      541 cgtgctggga ggctctgtcc acgcatccac ttacccgctg agaaattgcg gtgcgcttcg
      601 cggagcgcac tctaaacacc gattattctc tgaggcttac ctccgatgcg acacttggag
      661 acgagaattg agggtgaggt tgggacaatt accattgacc atccggcaaa acgcaacgcc
      721 ctctcacgac aacttgtcga tgctatcgtc gaagcgctag atcgcctttc acaagagcgc
      781 gtacgcgcaa tcgtgctgcg agccaagccg ggagtgaaag tatggtcgtc gggtcatgac
      841 gtagaggaac ttccggaggg cggtcgagac cctctaggat gggacgatcc tttgcgctac
      901 ctggtgcgtg ctatcgagga acatccggcg ccaataatta ccctgataga gggtggcgtg
      961 tggggaggcg catgtgaagt cgtcttcgct tgtgatctaa tcgttgctgc tccaagtgcg
     1021 actttcgccg taacacctgc gagactcggt gtgccctaca atgtttcggg catgctaacg
     1081 tttctcaatg cctgttccct ccggatcgtg aaagaaatgg cgtttacggc gaagccggtg
     1141 tcggccgaac gcgccgaacg tcttgctatc atcaatcaca tggtcgcggt ggagcgtctt
     1201 gagtcttttg tctatgagct tgcccaagat atcgcggaga atgccccgct tgcagtttcc
     1261 gtaatgaaag agcaattacg tatgcttgct ggtgctcatc cgatgagccc ccgtcgtttc
     1321 gaacgcgtcc agggtttacg gcgcatcgtc tacgacagcg aggattatgc ggagggtatt
     1381 cgcgcgttca aggaaaagcg taaaccgctt ttttccggtc gctagccagg ccgtgtactc
     1441 ataaatcgag attggtagca gcggttcgtc actgtccgct ttgcctccaa gcgtctgctt
     1501 tgtgccataa gcagacgatt gcgattcccg aagcgcaagc cgtgcaagac ttattcgcaa
     1561 accgacgtct ttcgctcttg aaggtccaga tgcaaatgag ttcgatgcgt gtcgttggcc
     1621 tctgggccaa gcaccgtgga gaatattttg cagccgccat catgcgcgag gcgcaggaac
     1681 ttggctgcaa cttcagagtc atcagcggac gtcggactcg gactggccac ggttgtgctg
     1741 tttgaggccg gtgaagtcga gacgttcact acctgggtcg ctgcgccctt cgcatctgtc
     1801 ttctgtttgg cgagggagcc cgtaacgcct gcagtggcct tcttcgcctt tgcggcggca
     1861 attagatcgc gctgagtagg accccagccg tgcgtgacgt caacctttct gccgtcggca
     1921 agaacaaaca tcggcagatc gatcgcattg gcaaaagcat gttggctgag gtggccatcc
     1981 tgcaggttat agacgttgcg acatgcatag gacgaaccga tgatcttcga caccggcgat
     2041 gagaacgtct ttctagccgc gggctgcacg ctatccttaa gccagcgatc gagcccgacc
     2101 accatcgcgc agtccaggac cagaggcgga tcgaatgaaa ccttctctcc accaccgatg
     2161 ctcttcacaa gcaccggcgc gggcgcaccg caattgccat ctcggatcgg gtctaacgga
     2221 gcaacatcag cggtcacggg tgccagcgct tgtatgcact gcatgagcgc cgcctcgatt
     2281 tccttatccg tccaagtagc ggttggttcc tgaggcgctt tcccttcttg ctctggtgat
     2341 ggcgtcatag gcagccaatc tctcggtgat ccgagccaat ttcgggccgg ccccgaccag
     2401 agtaggccgg tcaagacggc gagcaaaaca atgccacaca ccgcgatgac ggaaaccaag
     2461 tctctaaagc ctgagtggtc gtcttcgttc atctcgacgt tcggatggca ctaggcccca
     2521 gagcggcgtt ctcattacaa acgtcgttaa cctgcttcag gttctctgcc agagatcccc
     2581 cttcatcgtc tttgatcttt gcgctcacct cgcgcgcgat taacgcgcac acctctttga
     2641 gcgtgccttt cgtttccgag taactgttca tgtaccagag cactagagcc accaaaccta
     2701 gacacacgag gaacaatttg cggagcaatc tccaccattc cgactcagcc cttagaagcc
     2761 gacgggtgtc agctgcatgc atggtctcca agcgtttcag cgcgtcctgt agccgctcca
     2821 cctcgtctga taggggccga tcccaatttt tctcgagcca aacctggaag ttctcctctt
     2881 ccgtcgtcat ggcgagtcga cgcccgcgag tcatttaaaa catccctgag atgaatgggg
     2941 tggcaaccca cactctcaac caagttccct gctaaagttt tacctctctt cggcttaaac
     3001 tcgcgggcgc ctgtgggacg atgtcagttg gctcgcgcat cttcctgagc ctttaaattc
     3061 agccctcaac agcacgcgtc cctatggcgc caacagcggg acgtgcgccg ctgcgtccat
     3121 cggagccaat ccagaaagcc tcggatcttc agcgatctcc acctgccgtc ggaacggccg
     3181 gaagccggag cgcttgtaga agccgagagc ggacgagtga tcgagggtgc aggtgtgcgc
     3241 ccaaaaacgc cggataggac gcgaccaggc acgttcaatg gcgcggttca tcagccatcg
     3301 tcctgctggt ccgccgatta gcgttcggga cacgccgaag aaggccagtt cgcactcatt
     3361 cttctcccgg aaatcgagct caagcagccc cgccccgtcg ccgtcgtcag tcagcttgta
     3421 aacctccaca tcggcggagc cgatgatgac ggcaagctcg gcgtcggtaa gcagcaaccg
     3481 gctgaaccag agccagtcct cgccgacggc acgatagagt cgacggtact cggtcggatc
     3541 gcggcttacg gctaatgcac cggagcctcg ggctttctgc tttgctgata
//
LOCUS       DATDNW010000378         7849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1220968, whole genome shotgun sequence.
ACCESSION   DATDNW010000378 DATDNW010000000
VERSION     DATDNW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220968"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VI359_09005"
     CDS             <1..424
                     /locus_tag="VI359_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEY6974429.1"
                     /translation="RYFGYDDAIYASCRLVEILAKVQRPLSSLVADLPRTSVTPEIRV
                     DMPDTVKFDLVKQVQQRFADYSRSRQALGPDRLILRDVVTIDGVRAVFDDGWGLIRAS
                     NTQPALVLRFEATSLQRLEAIRAVVEGELAAAKLALGH"
     gene            372..1235
                     /locus_tag="VI359_09010"
     CDS             372..1235
                     /locus_tag="VI359_09010"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY6974430.1"
                     /translation="MLLLKENSRLRNWPSDIRLLPHLSVRRVLACLWFLAISLSCSLP
                     FAGSLLAQDASDGGTRSFQVGERLTYEITWLNILAGTAVMEVGDAGMVGNRPLAKLVT
                     TAQSSPMVTKFYPVDNRVESLVDPTTLQSDRMTFRRREGKRKNDFEYTFHHREGTVTV
                     IKDGETETLAIPPDTLDIISCLYHVRTTLPLKPGSSLVMNVHHDKKNYKLNVRVEEVE
                     SIEGSWGKSEAARVLVIMPFQGIFLNQGNIKVWFTTDDRKIPLRMKAKVIIGSVVADL
                     VGGIPGLVHPK"
     gene            1369..2373
                     /gene="fbp"
                     /locus_tag="VI359_09015"
     CDS             1369..2373
                     /gene="fbp"
                     /locus_tag="VI359_09015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY6974431.1"
                     /translation="MVPPSMTLDGFLNAQKSTSSADAGSLRRLFAAVGLAGKRLAQDL
                     RRAGLLNILGTTGETNVQGEAIKKLDQIANTTFVEAFQQSGVVCALGSEEMENPLLMP
                     DRWPNGPYVVLIDPLDGSSNTDVNMPLGSIFSILTCRHTDRPPNAEDLVRKGTDQIAA
                     GYLFYGPSTMLVFTIGQGVVGFTLEPETGDYLLSHASIRIPPRGKVYAANEGNYQKWT
                     GGMKQYVDRLKMRDKAAGRPYSGRYSGCMVADVHRLLLSGGIYLYPGEIDKPEGKLRL
                     LYEANPLAMVVEQAGGAASTGTMRIMDVEAKMLHQRVPLIIGSMEDVREAEACIQGRV
                     "
     gene            2412..3335
                     /locus_tag="VI359_09020"
     CDS             2412..3335
                     /locus_tag="VI359_09020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY6974432.1"
                     /translation="MADRVQEILSWYGSDNAGTKTNIARLLRSGKLAGSGKLVILPVD
                     QGFEHGPARSFAPNPPGYNPHYHFQLAIDAGCNAYAAPLGFLEAGAAEFAGRIPLILK
                     LNNHDVLHDEKDPLPSVTGSVGEALRLGCSAVGFTIYPGSAHCNAMYEQLRAIAEEAK
                     ASGLAVVVWSYPRGSALSKEGETAMDVVAYAAQIAAQLGAHIIKVKLPTAHLEQAAAK
                     KVYETTQIPIKTLAERVKHVVQSAFDGRRIVIFSGGAKSEDQNVFEEARAIRAGGGFG
                     SIIGRNSFQRPKAEAIKFLQTIMGIYAGEIQ"
     gene            3378..4916
                     /locus_tag="VI359_09025"
     CDS             3378..4916
                     /locus_tag="VI359_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY6974433.1"
                     /translation="MNEWPRNVVVSRRAWVIPGLLVGIGIVIGVVVASDLGWLPSGTA
                     GPEPKPNVTVRPVATAPQSPVTGGSARNFVEIAKLVKPTVVNISATRSGRGGEGFQGS
                     PFDDPFFRRFFGEEFFKRDQPKERKERGQGSGVIVDANGLIITNNHVVNKADEIRVFL
                     SDKREFKAKLIGTDAKTDVAVVKIEASGLPVIPWADSDQLEVGEFVLAVGNPFGLTQT
                     VTMGIVSAVGRASMGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIFSQSGG
                     NMGIGFAVPSNLARSMMEQLVKTGKVVRGWLGVSIQELTPELATQFGISDTKGVLVSD
                     IMDDSPAKKAGVERADVIVEFDGKPMDSPTHLRNAVAQTPIGKKVSLKVIRDKKPKTL
                     DVTIAEQPKSVAQAGSEDNGDVTAPTGVLSDLEVRELTDELAGRYGLKSTERGVVITR
                     VKPGSTAEEMGVREGDIVLEVNRKQVTSLKSYEKLVSGLTKDQAVLLLLKRQGRTMYL
                     TLRP"
     gene            4970..5995
                     /locus_tag="VI359_09030"
     CDS             4970..5995
                     /locus_tag="VI359_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY6974434.1"
                     /translation="MVSRAIFVLLSALAGMALFLRADSASASYWLYGFGIGAATGGLI
                     VAGEYTLRNLSFGIIVGGTAGLAVGLLLTGLVEWVGGEIFDVETFLFHIGGLVFLLGL
                     PYLGLVLGARFGKEKFPSPQQKFFEVNGSAGYPKVLDTSVIIDGRVADLCETGFLEGA
                     FLVPHFILDELQHIADSSDSLKRARGRRGLDILNKIQKMADVDVRIVDEDFPQVKEVD
                     AKLVVLAKQMHAKIITNDLNLNKVAELQGVRVLNINELCNALRPVVLPGEAIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRCIGRNVDVVVTSVLQTTAGRMIFTRLKEDTERE
                     ELQVARG"
     gene            5988..6701
                     /gene="ispD"
                     /locus_tag="VI359_09035"
     CDS             5988..6701
                     /gene="ispD"
                     /locus_tag="VI359_09035"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY6974435.1"
                     /translation="MGSRVVAIVPAAGRGLRMGGSVPKQFLSLGGEPLVVHSLRILQA
                     AAIVDEIILAVPATDLDYCTNDLVKQHGFSKVRKVVAGGLERQDSVRQALGHVTEETE
                     IVLVHDAVRPFLTGQMVNDVIAMADKQGAAIIALPMRDTVKQVDSDHVIQRTVDRRPL
                     WLAQTPQAFRRQWLQEAHRKAHAEGIHATDDAYLVEWLGHSVMVVEGSGENIKVTRPE
                     DMVIGEAILASRSVVRQAS"
     gene            6704..7198
                     /gene="ispF"
                     /locus_tag="VI359_09040"
     CDS             6704..7198
                     /gene="ispF"
                     /locus_tag="VI359_09040"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY6974436.1"
                     /translation="MATKIRIGYGYDVHPLGAGRALILGGIDIPHPKGLLGHSDSDVL
                     VHAVCDALLGAMGEGDLGRHFPSSDPRFKNISSLKFLEDVAAKLGKKGYRIVNIDTVI
                     VAQAPRLSTFLAAMQKRMADILGLDPDLINVKVKSGEGLDAVGREEGMTAHAICLIES
                     IQSS"
     gene            7214..>7849
                     /gene="cysE"
                     /locus_tag="VI359_09045"
     CDS             7214..>7849
                     /gene="cysE"
                     /locus_tag="VI359_09045"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY6974437.1"
                     /translation="MLNAIRQDLQAVFDRDPAATSRFEVILTYAGFHALLAYRVSHWL
                     KVHRVPFVPRAISQLARWLTGIEIHPAAQIGTGFFIDHGMGVVIGETAEIGDYVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGIKIGDNVKIGANSVVLKSVPPNSTV
                     IGVPARIIKTQGERMPDATMDHTNMPDPISDRFTALEQELIELRKKLENRDE"
BASE COUNT         1711 a         2013 c         2430 g         1695 t
ORIGIN      
        1 tcgctatttc ggctatgacg atgcgattta cgcgtcctgt aggctcgtag agattcttgc
       61 aaaggtacaa cggccgctct cctccctagt tgcagacctt ccacgcacct ccgtcacccc
      121 ggaaattcgc gtggatatgc cggacacggt caaatttgat ctcgtgaaac aggtgcagca
      181 gcgcttcgcg gattattcgc gaagccggca ggcccttggt ccagacagac tcatcctgag
      241 ggatgtcgtc accatcgacg gagtccgggc cgtctttgat gatggatggg gtctgatccg
      301 tgcctcgaac acgcaaccag ctctcgtcct gcggttcgaa gccacgtcct tgcaacgtct
      361 tgaagccatc cgtgctgttg ttgaaggaga actcgcggct gcgaaactgg ccctcggaca
      421 ttaggctttt gccccacctc tccgtgcgac gagtcctcgc ctgcctctgg tttctggcga
      481 tatccctttc gtgttccctc cctttcgccg ggagtctcct tgcgcaggac gcatccgacg
      541 gcgggacacg atcattccag gtcggtgaac gtctgactta tgagatcacg tggctgaata
      601 ttctggccgg caccgcggtg atggaagttg gagatgcggg gatggtcggg aatcgcccgt
      661 tggccaaact tgtgacgacg gctcagtcga gccccatggt gacgaaattc tatccggttg
      721 ataatcgggt ggagtccctg gtcgatccca cgacgttgca gtcagaccgg atgaccttcc
      781 ggcgacggga aggtaagcgg aagaacgatt tcgaatacac cttccatcat cgagagggaa
      841 cggtgaccgt catcaaggac ggggagaccg agaccttggc gataccgcct gatacgttgg
      901 atatcatttc ttgcctctat catgtgcgga ccacattgcc gttgaaaccc ggctcctctc
      961 tggtgatgaa tgtccaccac gacaagaaaa actataagct gaatgtccgt gtggaagagg
     1021 tggaaagcat tgaaggttca tggggaaaat ctgaagcagc gcgggtcctt gtgatcatgc
     1081 ctttccaagg gatctttctc aaccagggga acattaaggt ctggtttacc accgacgacc
     1141 ggaaaatccc cctcagaatg aaagccaagg tcatcatcgg gtcggttgtc gcggaccttg
     1201 tggggggaat tccaggactc gttcatccca agtgagagag ccgaacattc attgccggtc
     1261 cgttctgaag tcgttataat taccccagtc attatcccaa gaggcggagg ttcgcctttt
     1321 tataatctct tgattgggcc tgtgcccaaa gcacgcgaga tcgtttgtat ggtcccccct
     1381 tccatgaccc ttgatgggtt tctcaacgcg cagaaatcca catcttcagc tgatgctggg
     1441 tcattgaggc ggctctttgc cgctgtcggt ctggctggaa agcgtttggc gcaggacctt
     1501 cggcgggctg gtcttctgaa tatcctcgga acaaccggtg aaaccaatgt gcagggagag
     1561 gcaatcaaga agcttgatca gatcgcaaac accaccttcg tggaggcttt tcagcagagc
     1621 ggggtcgtct gtgcgttggg atcggaggag atggagaacc ctctcctcat gccggatcgt
     1681 tggccaaacg gaccgtacgt tgtcttgatc gatccgttag acggatcctc caacacagat
     1741 gtgaacatgc cgctcggctc gatcttctcc attctgacct gtcgtcacac agacaggcct
     1801 cccaacgcag aggatctcgt gcgaaagggc acagatcaga tcgcggccgg ctacctgttc
     1861 tatggtccca gcaccatgtt ggtgtttaca atcgggcagg gcgttgttgg gtttacgctc
     1921 gaaccggaaa cgggagacta cctgctgtcg cacgcatcga tcaggattcc gccgagaggg
     1981 aaggtctatg cagcaaatga agggaattat cagaagtgga cgggcggcat gaagcagtac
     2041 gtcgatcgat tgaaaatgcg ggacaaggcc gctggccgcc cctacagtgg gcgttattcc
     2101 ggttgtatgg tcgcagatgt gcatcgcctg ttgctgagcg gcggaatcta tctctatccg
     2161 ggagagatcg ataagccaga aggaaagctg cggttattgt atgaggcgaa tccgctggcg
     2221 atggtggtcg aacaggcggg gggcgcggcc agcaccggca ccatgcgtat catggatgtc
     2281 gaagcgaaaa tgctccatca gcgggtcccg ttgatcatcg gcagtatgga ggatgtgcgg
     2341 gaggctgaag cctgcattca aggaagggtc tagattacag atgggcgaac gagggatggt
     2401 tgaggaatca catggctgat cgagttcaag aaattctgag ttggtacggc agcgacaatg
     2461 cggggacaaa aaccaatatt gcgcgtctgc tgaggtccgg caagctggcc gggagcggga
     2521 aattggtcat tttgccggtc gatcaaggat tcgagcatgg tccggcccgg agttttgccc
     2581 ccaatccgcc gggctataac ccgcactatc atttccagct ggccatcgat gccggatgca
     2641 atgcctatgc ggcgccgctt ggatttctgg aggccggggc ggcagagttt gccggacgga
     2701 ttcccctgat tctcaagctg aataaccacg acgtcctgca cgacgagaag gatccgttgc
     2761 cgtcggttac cggaagcgtg ggcgaggctc tccggctcgg ctgctcagcg gtggggttca
     2821 cgatctaccc cggctcggct cattgcaatg ccatgtacga acagctacga gccatcgccg
     2881 aagaagccaa agccagtggc ttggccgttg tggtgtggtc gtatccccgc ggttcggcgt
     2941 tgagcaagga aggggaaacc gcaatggatg tggttgcgta tgctgcacag attgccgctc
     3001 agctgggtgc ccatatcatc aaggtcaaac tccctacggc acatcttgaa caagccgcgg
     3061 cgaaaaaagt gtatgagacg acacagattc cgatcaagac cctcgcggag cgagtgaagc
     3121 atgtggttca aagcgcattt gacgggcggc ggattgtcat tttctccggg ggtgcgaaaa
     3181 gcgaagatca gaacgtgttc gaggaggctc gtgccattcg ggccggcgga gggtttggtt
     3241 ccatcatcgg gcggaattcg ttccaacggc cgaaggccga ggcgattaag ttccttcaga
     3301 ccatcatggg gatctatgcc ggtgagattc agtgatcgtc gggtgcggga attgcgcaac
     3361 ttggatgagt ggcatcaatg aatgaatggc cgcggaatgt cgtcgtgtct cgtcgggcct
     3421 gggtgattcc ggggttgctc gtggggatcg ggatcgtgat cggggtggtg gtcgcgtccg
     3481 atctcggctg gcttccttcg gggacggcgg gaccggagcc gaagcccaac gtgacggtcc
     3541 gtccggttgc caccgcgccg caatcgccgg tcacgggtgg aagtgcacgc aacttcgtcg
     3601 aaattgccaa actggtcaaa cccactgtgg tgaatatctc cgccactcgc agcggtcgtg
     3661 gcggagaggg gtttcaaggt tctccgttcg atgacccgtt ttttcgtcga ttcttcggcg
     3721 aagaattctt caagcgcgac cagccgaaag agcggaaaga gcgaggacag ggatccggtg
     3781 tcattgtgga tgccaacggg ttgatcatca cgaacaatca cgttgtcaac aaagccgatg
     3841 agatcagggt gtttttgtcc gacaaacggg agttcaaagc caaattaatc gggaccgatg
     3901 ccaagacaga cgtggcagtc gtaaaaatcg aggcgtcggg actgccggtg attccatggg
     3961 ccgactccga tcagctggaa gtaggagagt tcgtcctcgc ggtcggtaat ccgttcggcc
     4021 tcacccagac tgtgacgatg gggattgtga gtgcggtcgg acgggctagt atgggtattg
     4081 cggagtatga ggatttcatc caaacggatg cggcaatcaa tccaggtaat tcgggcggtg
     4141 cgttggtgaa cgtacgcggc gaattggtcg ggatcaatac tgcgatcttc agccagagcg
     4201 gcggcaatat gggaatcggg tttgccgtgc cgagcaatct tgcccgttcg atgatggagc
     4261 aactggtcaa gaccggcaag gtggtccgtg gctggttggg cgtgtcgatc caggaactca
     4321 cgccggagtt ggcgacgcaa tttggcatca gcgacacgaa gggcgtcctt gtcagcgaca
     4381 ttatggacga cagtccggcc aagaaagccg gcgtagaacg ggctgatgtc atcgtggaat
     4441 tcgacggcaa accgatggat tcaccgacgc atctccgcaa cgctgttgcg cagacgccga
     4501 tcggcaagaa agtgtcgctc aaggtcattc gcgacaagaa accgaagact ctggatgtga
     4561 cgattgccga acagcccaag tcggtcgctc aagctggctc agaggataac ggagacgtca
     4621 cggcgccgac gggggtactc tctgatctcg aggttcgtga gttgaccgac gaattggcgg
     4681 gccggtatgg actgaaatcg accgagcgcg gtgtcgtcat tacccgggtg aaacccggta
     4741 gtacggctga ggagatgggt gtgcgtgagg gcgacattgt actcgaggtg aatcgtaagc
     4801 aggtgacctc gctcaagagt tacgaaaagc tcgtctctgg actgaccaag gatcaagcgg
     4861 tccttttgct gttgaagcga caaggccgaa cgatgtactt aactctgcga ccgtagggac
     4921 tgctcgaccg gtctcgacgg gggcaggatt tacgggggga ggtaaaggta tggtatcgcg
     4981 agccatattc gtccttctca gtgcgttggc tggcatggca ctctttctga gggccgatag
     5041 cgccagcgcg tcctattggc tgtatggatt tggaatcgga gcggcgaccg gagggttgat
     5101 tgttgccggc gagtacacct tacgtaacct ttcattcggt attatcgtgg gcgggacagc
     5161 agggctggcg gtggggctgc tgttgaccgg tctggtcgag tgggttggag gcgaaatctt
     5221 tgacgttgaa acgttcctgt tccatatcgg ggggctcgtc ttcctactcg gtttacccta
     5281 cctgggactg gtcctcggcg cacggttcgg aaaagagaag ttcccgagtc cccaacaaaa
     5341 gtttttcgag gtgaacggat cagcgggcta cccgaaagtt cttgatacca gtgtcatcat
     5401 cgacgggcgc gtggcggatt tgtgcgaaac cggatttctc gaaggcgcct tcctcgtgcc
     5461 gcattttata ctggatgagc ttcaacacat cgccgattca tccgattcgt tgaagcgcgc
     5521 gagagggcgg cgaggactcg atatcctcaa caagattcaa aagatggcgg atgtggatgt
     5581 ccgaatcgtc gatgaggatt tcccgcaggt gaaagaagtc gacgcgaagc tggtcgtgct
     5641 ggcgaaacag atgcacgcca agatcattac gaacgatctc aatttaaaca aagtcgctga
     5701 gctgcaaggt gtgcgcgtct tgaacatcaa cgaactctgt aatgcgctcc ggccggttgt
     5761 gcttccgggc gaggccatcc gcgtctttgt gttgaaagaa ggcaaagaag ccgggcaggg
     5821 cgtggcctat ttggacgacg gaacgatgat cgtggtagat aacgcacgac gctgtatcgg
     5881 acgaaacgtc gacgtcgtgg tgaccagcgt tttacaaacc acggcaggtc ggatgatctt
     5941 tacgcgcctc aaggaagaca ccgagcgaga ggagctgcaa gtcgcccgtg ggtagccggg
     6001 ttgtcgcgat agtgccggcc gccggtcggg gactgcggat gggcgggtcg gtcccgaagc
     6061 aatttctttc attgggcggc gagccgctcg tcgtccattc cctgcgaatc cttcaggctg
     6121 cagccatcgt cgacgagatt atcctcgccg ttcctgcgac ggaccttgac tattgcacca
     6181 atgatcttgt gaaacaacat gggttctcca aggtcaggaa agtggtagcg gggggacttg
     6241 aacggcagga ctctgttcga caggcgctcg ggcacgtgac ggaggagacg gaaattgtgc
     6301 tggtacacga tgccgtccgg ccttttctca ccgggcaaat ggtcaacgat gtcatcgcaa
     6361 tggccgataa acagggagcc gccatcattg cccttccgat gcgggacacc gtcaaacagg
     6421 ttgattcgga tcatgtcatc cagcgaaccg tcgatcgccg tcctctctgg ctggctcaaa
     6481 caccgcaggc attcagacga cagtggctgc aagaggccca tcggaaagcc catgccgaag
     6541 gtatccatgc gacggacgat gcgtatctcg tcgaatggct tggccattcg gtaatggtcg
     6601 tagagggaag cggggagaat atcaaagtga ccaggccgga agacatggtg atcggggaag
     6661 cgattctggc ctctcgatca gtagtacggc aagcgagctg agcatggcga ctaaaatacg
     6721 gatcggatat ggatatgatg tgcatccact cggcgccggg cgagcgctca ttctgggtgg
     6781 tattgacatt ccgcacccca aagggctcct gggccattcc gactccgatg tgctcgttca
     6841 tgccgtctgt gatgccctgt tgggggccat gggcgaaggg gatctcggcc ggcacttccc
     6901 aagctcggat ccgaggttca aaaacatttc cagtctgaaa tttctggaag atgtcgcggc
     6961 taaactcggg aagaagggct atcgcatcgt caatatcgac acggtgatcg tggctcaagc
     7021 ccctcgattg agcacctttc ttgctgccat gcaaaagcga atggcagata tcctgggcct
     7081 ggacccggac ctgatcaacg tcaaggtgaa gagcggcgaa gggttggatg ccgtgggccg
     7141 agaagaaggg atgacggcgc acgccatctg tttgattgaa tcgatacaat catcgtagga
     7201 tgtatcctct tttatgctga acgcgatacg acaggatctg caggcagtct ttgatcgcga
     7261 cccagccgcg accagccggt tcgaggtcat tctgacgtat gcgggttttc atgcgttgct
     7321 ggcctatcga gtatcccact ggttgaaggt tcaccgagtg ccattcgttc cccgagccat
     7381 ctcgcaactt gcccgatggc taacggggat tgaaatccac ccggccgcgc agatcggaac
     7441 cggatttttc atcgatcacg gcatgggtgt cgtgattggc gaaacggcag aaatcggcga
     7501 ctacgtgacc ctttttcaag gtgttacgct cggcggcacc ggcaaggagc gtggcaaacg
     7561 ccatcccacg ctcggcaatc atgtggtggt gggtgcggga gccaagattc tcggcggcat
     7621 caagattggg gacaatgtta agatcggcgc caactccgtt gtgctcaagt cggttccgcc
     7681 gaattcaacc gttatcggtg tgccggccag aatcatcaag acacaaggtg agcggatgcc
     7741 cgatgcgacg atggatcaca ccaatatgcc agaccctatc agtgatcgtt tcaccgctct
     7801 cgaacaggaa ttgatcgaac tccgcaagaa actcgaaaac cgcgacgaa
//
LOCUS       DATDNW010000379         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1355266, whole genome shotgun sequence.
ACCESSION   DATDNW010000379 DATDNW010000000
VERSION     DATDNW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355266"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VI359_09050"
     CDS             complement(<1..590)
                     /locus_tag="VI359_09050"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY6974438.1"
                     /translation="MNRPIDNQHVIEIKALPSPRTIKTKLPITDQAAALVVETREAIR
                     RILHGQDRDRLLVIVGPCSIHDPEIAYEYAVKLKPVADTLRDRLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIATGMELARTILLKINQCGIPCATELLDPVTPQYIADLISW
                     TAIGARTTESQTHREMASGVSMPVGFKNGTEGSLQVA"
     gene            850..1182
                     /locus_tag="VI359_09055"
     CDS             850..1182
                     /locus_tag="VI359_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974439.1"
                     /translation="MSQCPPAQSLFLMCRAGLSVGLVLLLLAIAPFAVAQDVHHELAA
                     ADSDGHEHSDHDLCQWVQHHTSGSLTIAIPSFACWGTISDEVWFPVTVLVSSSYFSVS
                     PSRAPPRS"
     gene            1284..>2685
                     /locus_tag="VI359_09060"
     CDS             1284..>2685
                     /locus_tag="VI359_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY6974440.1"
                     /translation="MLPTSRIDVRFELVGVLVLIVSGIVGELGGVFAADTDLRTIIGW
                     VQNQDLRRVDQVIVQVRDQEGNVAAQGVTNQAGEFTITVSQEGTYSVSAVQDTYKSEF
                     VVVKIGAERPAPVTLTLAVTREIALEIVSPLPAIQYKASSETYQLNRKDIEVLPRGNN
                     NTVAEVLQTVPSVVYGGLGQAHIRQDHANQQFRIDGVPIPEGVSSTFTDVISPRMWER
                     ADIILGGMEAQYGNKTAIVVDITSKSGTRPSFGSAQIFGGSNQTVNPSFEYGGTVGEK
                     FRYYVLNSYTTTNRGIEPPTVGHSVFHDQSERNQTYLRGDYQHDNRNSLSWVFLNAVA
                     KYQIPTIPGLDVNADVLPLLQAQDPTFSPAPSQAVNQNQKENTQYTHLVWRHDVNASN
                     LFQLAGFFRNSYADFTTDPFNTLAYLPDAQTANQTRKAYSLGTRLDYTWIPNPRHLVK
                     TGFQFEYTNAQNQFQLF"
BASE COUNT          585 a          735 c          745 g          620 t
ORIGIN      
        1 gccacttgaa ggctgccttc cgtgccgttc ttgaagccga ctggcatcga tacgccgctg
       61 gccatttcgc gatgagtctg actctccgtc gtgcgcgctc cgatcgccgt ccagctaatg
      121 agatccgcga tgtactgggg cgtcacggga tcgaggagtt cggtggcgca agggatgccg
      181 cattggttga tcttcaagag aatagttcga gccaactcca taccggtggc gatgtcacag
      241 gtgccgtcga gatggggatc gttgatcaga cctttccacc cgaccgttgt tctcggtttt
      301 tcaaaatacg tgcgcatgac aatcaacagt cgatcacgta gcgtatcggc gaccggcttc
      361 aacttgactg catattcgta ggcgatttct ggatcgtgaa tggaacaggg tccgacgatg
      421 acaagcagcc gatcacggtc ttgcccatgc agaatccggc ggatggcttc gcgagtttcc
      481 acgacaagag cggcggcctg atcggtaatc gggagcttcg tcttgatcgt ccgaggagac
      541 ggaagagctt tgatttcaat aacgtgttga ttatcgatcg gtcgattcat ggtccggccc
      601 tttcaggttt gcactcgagc gcgaaaaggg gtacagacta accaaatctt caagaaaagt
      661 ctacacggaa aactgtaacg caatgaatta taagatgatt ttatctcatc gtaggaggcg
      721 ggccttcgct tcaacagagc cggaagagct catacagtaa atcaacgtgg tgtgagggtg
      781 atcgaacatc atgattgatt atctgcgaat tgacaccccc cttgcgttgt gttactcagt
      841 gcgccctcaa tgagccaatg tcctcccgca caatcgctct tccttatgtg ccgggccggt
      901 ctgtctgtcg gcttggtcct gcttctcctg gcaattgctc cctttgccgt cgcgcaggac
      961 gtgcaccatg aattggccgc tgctgatagc gacgggcatg agcattcgga tcacgatctc
     1021 tgtcagtggg tgcagcacca tacgtccggt tcgctcacga tcgccattcc gtcatttgct
     1081 tgctggggaa cgattagcga tgaggtatgg ttccccgtca cggttctcgt ttcctcttca
     1141 tacttttcag tcagtccctc acgagccccc ccacgttcct aactgcgtga acgacgtgca
     1201 ggcgttctgc tccgccagtg gactctgtct acgggttgca ggcgcacgca tcattcatgt
     1261 cattcggata cgtggaggag ctgatgctcc cgactagtcg tattgatgtt cggtttgagt
     1321 tggtaggggt cctcgtattg atcgtgtcag gtattgtcgg tgaactcggg ggtgtctttg
     1381 cggcggacac tgacctccgc acgattattg gttgggtcca gaatcaggac ctgcgtcggg
     1441 tagatcaagt catcgtgcag gtacgcgatc aggaaggcaa cgtggctgcg cagggggtca
     1501 cgaaccaggc tggtgaattc acgattacgg tctcacagga agggacatac tcggtcagtg
     1561 ccgttcagga tacttataag agcgaattcg tggtggtaaa gatcggtgcc gagcgtccgg
     1621 caccagtgac gctgacgctg gccgtgacac gggaaatcgc cttggagatt gtctcaccgt
     1681 tgccggcaat tcaatacaag gcatcgagtg aaacgtatca gcttaaccgg aaagatatcg
     1741 aggtcctccc gcggggcaac aataataccg tggctgaggt tctgcagaca gttcccagcg
     1801 tcgtctatgg cggtctagga caagcgcaca tccgccaaga ccacgcgaac caacagttcc
     1861 ggatagacgg ggtgccgatt cccgaaggag tcagttcgac cttcactgat gtgatttcac
     1921 cccgtatgtg ggagcgcgct gatattatcc tcggcggcat ggaggcgcag tacgggaaca
     1981 agaccgcgat cgtggtcgat atcaccagca agagcgggac gcgtccttca ttcggttcgg
     2041 cccagatatt tggaggctcg aaccagaccg tgaacccgtc gttcgagtac ggcggcacgg
     2101 tcggtgagaa attccgctac tacgttctga acagctatac cacgaccaat cgcggaattg
     2161 agccgcctac ggtcggccac tcggtctttc acgatcaaag cgagcgcaac caaacctatc
     2221 tgcgaggtga ttatcagcat gacaaccgaa acagcttgag ctgggtgttc ttgaacgcgg
     2281 tcgccaagta tcagattccg accatcccgg ggttggacgt gaatgcagat gtcctgccgc
     2341 tccttcaagc gcaggacccc accttctccc ccgccccctc tcaggcggtg aaccagaacc
     2401 agaaggagaa tacccagtat acacacctgg tctggcgaca tgatgtgaac gccagcaatc
     2461 ttttccagct tgccggcttt ttccgaaatt catatgcgga cttcactacc gaccccttca
     2521 acacgctggc ctatttgccc gacgcccaga cagcgaatca gacaaggaag gcctactcac
     2581 tcgggacgcg cctcgattac acctggatcc cgaacccgcg tcatcttgtg aaaacgggtt
     2641 tccagttcga gtatacgaac gcgcagaatc agttccagtt gtttg
//
LOCUS       DATDNW010000380         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_266221, whole genome shotgun sequence.
ACCESSION   DATDNW010000380 DATDNW010000000
VERSION     DATDNW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266221"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="rbfA"
                     /locus_tag="VI359_09065"
     CDS             <1..418
                     /gene="rbfA"
                     /locus_tag="VI359_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY6974441.1"
                     /translation="RSSLRAMMKTGYKRADRVADQIRMEVADILMRKIKDPRVRSVTV
                     TDVELTNDLRIARVFVTAMGTDAEEREIFAGLAKASGFVRSELGRRLTLRYLPEVVFV
                     KDVSGPRGDRVLRLLDELQGKDEGSIGSSTGHTGSA"
     gene            439..1392
                     /gene="truB"
                     /locus_tag="VI359_09070"
     CDS             439..1392
                     /gene="truB"
                     /locus_tag="VI359_09070"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY6974442.1"
                     /translation="MMHVGPRCAVIAGVLNINKEAGWTSHDVVAKVRHLLEGVKVGHA
                     GTLDPMATGVLPVLVGRATRLAEYLVEWDKEYEAVLRLGEITDTQDATGTVLERRSTE
                     AVTPEMIYTVVARFRGPLEQVPPMYSAVKVGGVPLYKAARAGKTIARAPRTVILHALE
                     ITAVHERDVTLRVVCSKGTYVRTLCADIGEVLGVGGHLHSLVRMRVGPFSLDQALTVD
                     DVATHHALGRLGQDLHSLDQVLGRLPCVEVDDQTAVRVKHGVPVPPEGIVSGYGTVTG
                     VKSGTPIRIHDSQRRLMAIGRSAESQSHPIRIEKVLMSQDS"
     gene            1424..1693
                     /gene="rpsO"
                     /locus_tag="VI359_09075"
     CDS             1424..1693
                     /gene="rpsO"
                     /locus_tag="VI359_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY6974443.1"
                     /translation="MALLKEAKTELIKQYRQHDKDSGSPEVQIAVLTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRDIDDMRYRAVIDRLGIRK"
     gene            1834..3954
                     /gene="pnp"
                     /locus_tag="VI359_09080"
     CDS             1834..3954
                     /gene="pnp"
                     /locus_tag="VI359_09080"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY6974444.1"
                     /translation="MAHTVELDIAGRTLRLETGRVAKQADGSIWATYGDTIVLATAVA
                     SQTAKPGVDFLPLTVDYQEKAYAAGKIPGGYFKREGRPSEKEVLTSRLIDRPLRPLFP
                     DGYYFETQVIASVLSADKTGSSDVIGITAASAALAVSNIPFNGPVAGVKIGRVDGRFV
                     VNPDLETLEKSELHLVVAGTADAVMMVEAGANGLPEATMLQAIELAHAEIKKIVAKIN
                     ELRVLAGNKPKRTVVTEQIDPALASQVKTLVAQGIRDAIMIPNKTARQERLDQLKNDT
                     VEKLKRPDDPNRERHVKLVFHGLEYTEVRNMILEKGSRADGRGPADIRPITCEVGILP
                     RTHGSALFTRGETQSLAVITLGTTEDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPL
                     RSPGRREVGHGALAERALKPVIPSKEVFPYTLRIVSDILESNGSSSMATVCGGTLGMM
                     DAGVPIKEPVAGIAMGLIKEGERVMILSDILGLEDHLGDMDFKVCGTKDGVTALQMDI
                     KIGGITPALMHQALEQAKAGRLHILSCMRKALDAPRPNLSAFAPRIFTMKVKQDKIRE
                     IIGPGGKTIRGIIAECGVKMNVEDSGVVTIASVDEASAMKAKEMVNRIVEEVEVGKTY
                     LGTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKSVSDEVAEGDQILVKVLEVDRQG
                     KIRLSRKETMPAPTGAGAKDPSAG"
     gene            3960..>4107
                     /locus_tag="VI359_09085"
     CDS             3960..>4107
                     /locus_tag="VI359_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY6974445.1"
                     /translation="MYHKLVLENGIRVVTERIPTLKSVTVGIWVNTGSRDEQPSQAGF
                     SHFIE"
BASE COUNT          933 a         1060 c         1278 g          836 t
ORIGIN      
        1 ccgctcgagc ttgagagcga tgatgaagac aggctacaag cgggccgatc gcgtagcgga
       61 tcagattcgc atggaggtgg cagatatcct catgcgaaag atcaaggacc cgcgtgtccg
      121 ttcggtgacg gtcacagacg ttgaattaac gaacgatctg cggatcgccc gagtgtttgt
      181 gacggccatg ggaacggacg ccgaggaacg cgagatcttt gcgggactcg caaaggcgag
      241 cggatttgtt cgcagtgagc tgggtcgccg gctgacactc cgttatctgc cggaagttgt
      301 ctttgtaaag gacgtgagtg ggccgcgagg cgatcgagtg ttgcggctct tggacgaatt
      361 gcagggtaaa gatgaagggt cgatcgggtc gtcgaccgga cataccggat cggcgtagtg
      421 tcggcattgg gtggatagat gatgcatgta ggacctcggt gcgcagtcat cgccggcgtg
      481 ttgaatatca ataaagaggc aggatggacc tctcacgatg tcgttgccaa ggtgcgtcat
      541 ctccttgaag gtgtgaaggt aggacatgcc gggacgctcg atccgatggc aaccggagtg
      601 ttacccgtac tcgtggggcg agccacgcgc ctcgccgaat atctcgttga atgggataaa
      661 gaatacgagg ccgtccttcg gcttggggaa attaccgata cgcaggatgc gaccggaacc
      721 gtgttggaac gccggtcaac cgaggccgtg acgccggaga tgatctatac ggttgttgca
      781 cgattccgtg gaccattaga acaggtcccg ccgatgtatt cagccgtaaa ggtcggcggg
      841 gttcccttgt ataaggcggc acgtgccgga aaaacaatcg cgcgcgcgcc gcggaccgtt
      901 atcctccacg ctcttgagat cacagcggtt cacgaacgcg atgtcactct tcgggttgtg
      961 tgttcgaagg gcacctatgt gcgcaccctc tgtgcggata ttggtgaggt cttgggagtt
     1021 gggggacatt tgcactcgct tgttcggatg cgtgtcggtc cgttcagtct cgatcaggcg
     1081 ttgacagttg acgatgttgc gacccatcac gcacttggtc ggctcgggca ggatctccac
     1141 tccttggatc aggtgttagg acgactgcct tgtgtggagg tggacgatca gacggccgtc
     1201 cgtgtgaagc acggggtgcc ggtgcctcca gagggaattg tgtcaggata cgggactgtt
     1261 actggggtga agtcaggcac gccgattcgt atccacgata gccagagacg actgatggcg
     1321 atcgggcggt ctgcggaaag tcagtctcac ccgatacgca ttgaaaaagt gctgatgagt
     1381 caggattcgt aataccagta tatcgaggag gaggaaacga ggtatggcct tgctgaaaga
     1441 agcaaaaacc gagttgatta aacaatatcg gcaacacgat aaggactccg gctcgccgga
     1501 agtccagatc gcggtcttaa ccaaccggat cacatatttg acggaacatt ttaagctcca
     1561 taagaaagat caccattcgc gccgtgggct cctcacgctg gtggggcgcc gccgtcggct
     1621 gctggactat ctccgcgata tcgacgatat gcgctatcgc gccgtgatcg atcgcttggg
     1681 catcaggaag tagaagcgca tctcgttgtc gtgccgatgc acgacaacga ggtgaaccag
     1741 tcgcacaggt gaacactgac agaagcttga gccgctcgcc gttctagctt ctcttcgtgg
     1801 gcatctgtgg ggcacaacca gaggagaccc tcgatggcac acacagttga actcgatatc
     1861 gccggacgga ctctacgact tgaaaccggt cgagtcgcca aacaagcgga cggatcaatc
     1921 tgggccacgt atggagacac gatcgtcctc gcgacggcgg tggcgtccca gaccgccaag
     1981 ccgggggtcg attttcttcc ccttaccgtc gactaccagg aaaaagccta tgccgccggg
     2041 aagattcctg gcggttattt taaacgtgaa ggccgtccgt cagaaaagga agtcctcacg
     2101 agccggctga ttgatcgtcc cctccgtccg ctgtttcctg atggatatta cttcgaaacg
     2161 caggtgatcg cgtcggtgct ctcggccgac aagaccggct cctctgatgt gatcggaatt
     2221 acagctgcct ccgcggcttt ggccgtatcc aacattccct tcaatggtcc ggtcgcgggc
     2281 gtcaagattg gtcgtgtgga cggccggttt gtggtgaacc ccgatctcga aacgttggag
     2341 aagagcgaat tgcatctggt ggtagctggc acggccgatg cggtgatgat ggtcgaagcc
     2401 ggcgcgaacg gattgccgga agccacgatg ctccaagcga tcgaattggc ccacgccgaa
     2461 atcaagaaga tcgtcgccaa gatcaatgaa ttgcgcgtct tggctggcaa caaacccaaa
     2521 cggactgttg tgacggagca gattgatccg gcgctcgcga gtcaagttaa gacactcgtc
     2581 gcacagggga tcagggatgc catcatgatt ccgaacaaga ccgcgcggca agagcgcctg
     2641 gatcaactca agaacgacac ggtcgagaaa ctgaagcgtc ccgatgatcc caaccgtgag
     2701 cgacatgtga agctggtctt ccacggactg gagtacacgg aagtccgaaa tatgatcctt
     2761 gaaaaaggat cgcgcgccga tggacgtgga ccagccgaca ttcgtccgat cacctgtgag
     2821 gtcggtatct tgcctcgcac acatgggtcg gccctcttta ctcgtgggga aactcagagt
     2881 ctggccgtca tcacactggg aacgactgaa gacgagcagc ggatcgatgc actggaaggc
     2941 gaatacacca ggacgttcat gcttcattac aactttcctc ctttcagcgt cggggaggcg
     3001 aggccgctcc gatcgcccgg acgtcgtgaa gtcggtcatg gcgccctggc cgagcgagcg
     3061 ctgaagccgg tgattccgag taaggaggtg tttccgtaca cccttcggat cgtgtctgac
     3121 attctcgaat ccaacgggtc gtcctcaatg gcgacagtct gtggcgggac attgggaatg
     3181 atggatgcgg gtgtcccgat caaagaacca gtcgcaggga ttgcgatggg attgatcaag
     3241 gagggagaac gggtgatgat cctttccgat atcctcgggc tggaagacca cctgggcgat
     3301 atggacttca aggtttgtgg aacgaaggat ggggtaactg cgctgcagat ggacattaag
     3361 atcggaggta ttacgccggc tcttatgcat caggcattgg aacaagccaa ggcgggcaga
     3421 ttgcacattc tgagctgtat gcggaaagcc ctcgacgcgc caagacctaa cctgtcggca
     3481 tttgccccgc gcatcttcac gatgaaggtg aagcaagaca aaatccgcga aatcatcggt
     3541 ccgggcggaa agaccattcg cggaatcatc gcggaatgcg gcgtaaagat gaacgtcgag
     3601 gacagcggcg tggtgacgat cgcctcggtc gacgaagctt cggccatgaa agccaaggaa
     3661 atggtcaatc ggatcgtcga agaggtcgaa gtcgggaaaa catacttggg cacggttcgc
     3721 aagatcatgg acttcggcgc gttcgttgag gtgctcccag gaaccgacgg actcgtgcat
     3781 atctcccaac tcgcccatca tcgggtgaag tctgtgtccg acgaagtggc ggagggggat
     3841 caaattctcg tcaaggtgct ggaagtcgat cgacaaggga agatccgcct cagtcgcaag
     3901 gaaaccatgc cggcccccac tggagctgga gccaaggacc catccgccgg gtaagcgcct
     3961 tgtaccacaa gctcgtcctg gagaacggta ttcgggttgt caccgagagg attccgactc
     4021 tcaagtcggt gactgtcgga atttgggtga ataccggatc ccgagacgag cagccttccc
     4081 aggcaggttt ttcccacttc atcgaac
//
LOCUS       DATDNW010000381         3638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_603182, whole genome shotgun sequence.
ACCESSION   DATDNW010000381 DATDNW010000000
VERSION     DATDNW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603182"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2896
                     /locus_tag="VI359_09090"
     CDS             <1..2896
                     /locus_tag="VI359_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY6974446.1"
                     /translation="FDQRIQAMLQLGCRRFKLPTGLMTRVQGKDLVITHVSSQDANIA
                     AGMVLPLDQTYCHATLMAEGPVCFEQASASEWRDHPGYATLGLECYIGTKLVGLNSIH
                     GTICFVAPTPYAARFTDADKDFLLLMAQWIGHEQDRQESERALKQHESLLRAVIDTAT
                     DAIFMKDTKGRYQLINAAGARTIGKPVEEIVGKTDLELFPPETARQLRENDQRVLLGG
                     SQHHFEAVLPFQGESRTFSSIKTPHRDPQGNVIGLVGVSRDITDQKLLEEQRSKSERL
                     FASFMENLPGLAWIKDTGGRYVYLNGAFERAFHVKLSDWKGKTDFDVWPQAVADQFTA
                     NDRQVLSTNTPILTIETAPQVDGVHASLVSKFPIPNEQGSPILVGGVAVDITERKQAE
                     EALRLTQFAVDRAADLAFWIDREARFAYVNDAACQRLGYTRHELLSLTVADVDPNYQT
                     ESWPQHWEALRRTGQLRFETMHRTKTGELYPVEVVANYATFEGKEYNFAFARDISEQK
                     RAEAALRDSEARWKTLFEHAGVGIAQLSLSGRFLRVNARLCQTLGYSAKTLCQHTFHE
                     ITHPDDLEFNLRLMNELVSSARPSFSIEKRYLRSDGVWLWANLTVSLVRSTSGAPAYF
                     IKVIEDISERKQAEEALQQSEARLHRFVADAPVGLVIMDAQKRILSANKAFCALTGYT
                     ESEIIGSTYALYTHPDDLPENLALTDEFFRGERTGYAFEKRYVRKPGDIIWVAVKTTS
                     TELPGHVGPLLLAVVEDITERKQATDERERISQDLHDDVLQSLYAVGMGLEATKGRLK
                     PISRSTAKRLEGSVAQLNGVIHEVRSFIPRMRTSARTDGNFEQALRSLVGSFTATGAG
                     DIAITIDAETAAALPLDHCRDVISIAKEAISNSLRHAKARRRTVTFHRHRSNLRLEIV
                     DNGSGFRPSRSRKGLGLSNMRTRAKKLGARLTIDSAPGQGTRIVLDLPVR"
     gene            2899..3576
                     /locus_tag="VI359_09095"
     CDS             2899..3576
                     /locus_tag="VI359_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY6974447.1"
                     /translation="MRPQRESPITILLVDDHEVVRIGLRALLDAVPGFRVVGEGATKR
                     EAITGAVRLKPTVALLDIRLPDGSGIDAAREILSACPATNVAFLTSYADDDSVLAAVL
                     SGAQGYILKDIGSDNLVKAVRTVASGKPLLDPRLTNQTRTWVKNLSSPGQLSLTPSLS
                     PQEQRLLPLIAEGHTNKEIAEAMQLSEKTVKNYLANLFSKLHISRRSQAAAFYVRRAT
                     SDGARTE"
BASE COUNT          811 a         1090 c         1037 g          700 t
ORIGIN      
        1 attcgaccag cgtatccagg cgatgctgca gttgggttgt cgtcgtttta agcttccgac
       61 cggactcatg acgcgcgttc agggcaagga tctcgtcatc acacatgtct cgtctcagga
      121 cgcgaacatc gccgctggca tggttctgcc actcgaccag acatactgtc acgccacact
      181 catggcagag gggccggttt gcttcgagca ggcgtcggcc tcagaatggc gagatcatcc
      241 gggctatgcg acgcttggcc tcgagtgtta catcggcacc aaactggtcg gactcaactc
      301 aatccatgga acgatctgtt tcgtcgctcc cactccgtac gccgctcgtt tcaccgatgc
      361 agacaaggac ttcctgttgc tcatggcgca atggatcggc cacgagcagg accgccagga
      421 atcggagcgg gctctaaaac aacacgagag tctgttgcgc gccgtcatcg acaccgccac
      481 cgacgcgatt tttatgaaag acacgaaggg ccgataccag ctcatcaatg cggctggcgc
      541 gagaacgatc ggtaaaccgg tggaggagat cgtcggaaaa accgatctcg aattgttccc
      601 gcccgagacg gcaagacagc tcagggagaa tgaccagcgg gtgctgctcg gcggaagcca
      661 gcatcacttt gaggcggttc tcccattcca gggagaatcc agaacgtttt catcgatcaa
      721 gacgccgcac cgcgaccctc aagggaacgt gatcgggctc gtcggtgtct cacgcgatat
      781 caccgatcaa aaactgctcg aagagcagcg gagcaagagc gagcgattgt ttgcctcgtt
      841 catggaaaac ctgccgggtc ttgcctggat caaagatacg ggcggacggt atgtgtatct
      901 gaacggagcg tttgaacgag cctttcacgt caagctgtcg gattggaaag gaaagaccga
      961 tttcgacgtg tggccacagg ccgtcgccga ccagtttacg gccaacgatc gccaggtcct
     1021 ctcgacgaat accccgatcc tgacgatcga aacggcacca caggtggatg gagtgcatgc
     1081 ctcgctcgtc agcaaattcc cgatcccgaa cgaacaagga tctccgattc tcgtcggagg
     1141 cgtcgcggtt gacatcaccg aacgcaaaca ggccgaggag gccctgcggc tgacgcaatt
     1201 tgcggtggat cgagcggccg atctcgcctt ctggatcgac cgcgaagcac gttttgcgta
     1261 tgtcaacgat gcggcctgcc aacgactcgg ctatacgcgt catgagttgt tgtcgctgac
     1321 ggtggcagat gtggatccga attaccagac ggaatcgtgg ccccaacatt gggaggcact
     1381 tcggcggaca ggccagcttc gcttcgagac catgcaccgg acgaaaaccg gcgaactcta
     1441 tcccgtggag gttgtcgcca actacgccac attcgaaggc aaggagtaca acttcgcctt
     1501 tgcccgcgac atctccgaac agaaacgggc agaggccgcg ttgcgcgaca gcgaagcccg
     1561 ttggaagacg ctgtttgagc atgcaggagt cggcattgcc cagctcagct tgagtggacg
     1621 attcctgcgc gtgaatgcac gcctctgcca gacactcggt tattccgcca aaacgttgtg
     1681 tcaacatacc ttccacgaga tcacacatcc ggacgatttg gagttcaatc tccggctcat
     1741 gaatgagctg gtcagcagcg cgcgcccttc cttctcgata gagaaacgct atctcagaag
     1801 cgacggcgtg tggctgtggg ccaatctgac cgtttcgttg gttcgctcga cctccggcgc
     1861 cccggcctac ttcatcaagg tgatcgaaga catctcggag cgcaagcagg ctgaagaggc
     1921 acttcaacaa agtgaggcgc gactccatcg attcgtggcc gacgcgccgg tcgggttggt
     1981 catcatggac gcacagaagc ggatcttgag tgccaacaag gccttttgcg cgctgacagg
     2041 ctatacggag tcagaaatca tcgggagtac ctatgccctg tatacgcacc ccgacgatct
     2101 gccggaaaac ctggcattaa ccgacgaatt tttccggggg gaacggacgg gctatgcctt
     2161 cgaaaaacgt tacgtcagga aacccggcga catcatctgg gtggccgtca agaccacgag
     2221 caccgaactg ccgggtcacg tcggccccct cttgctcgcc gtggtcgaag atatcaccga
     2281 gcgaaagcag gcgacggatg aacgcgaacg gatcagccaa gatcttcatg acgacgtgct
     2341 ccaatcgctg tatgcggtcg gcatgggatt ggaagcgacg aagggccgac tcaagccaat
     2401 ctcccgatcc accgcaaagc ggcttgaagg gtccgtcgcc cagctcaacg gtgtcattca
     2461 tgaagtgcga agctttattc ctcggatgcg gacgtccgct cggacggatg ggaatttcga
     2521 gcaggccctt cgatccctgg tcggatcttt cacggcaacc ggcgccggtg acatcgcaat
     2581 cacgatcgac gcggagactg cggctgctct tccgcttgac cattgtcgag acgtcatcag
     2641 catcgcaaaa gaagccatca gcaacagcct gagacatgcg aaggctcgtc gccgcacggt
     2701 cacctttcac cgacaccgga gcaacctgcg gttggagatc gtcgacaacg ggtcgggatt
     2761 tcggccgagc cggtcgcgga agggactggg gctgtccaac atgcgaacca gggcgaaaaa
     2821 attgggggca cggttgacga tcgattccgc gccgggccaa ggcacccgca tcgtcttgga
     2881 tcttcctgtg aggtgagtat gcgccctcag cgggagagcc ccatcaccat cctgttggtc
     2941 gacgatcacg aagtcgtccg gatcgggtta cgtgcgttgt tggatgccgt cccgggattc
     3001 cgagtcgttg gtgaaggagc aaccaagcga gaagccatca caggcgccgt tcggctcaaa
     3061 cccaccgtcg cgctgttgga cattcgcctt cctgacggaa gtggaattga cgccgcccgt
     3121 gaaattctct cggcctgtcc ggccacgaat gtcgccttcc tgacgagtta cgccgacgac
     3181 gacagtgtgc tagccgccgt cctctcaggc gcccagggat acatcttgaa ggatatcgga
     3241 tctgacaatc tcgtgaaggc ggtacgtacc gtcgcgagcg ggaaacctct gttggatcct
     3301 cgtctcacca accaaaccag aacctgggtc aaaaacctct ccagtcccgg ccagctttcc
     3361 ttgacccctt cgctctctcc gcaagaacag cgccttctgc ccctgattgc cgaaggtcat
     3421 accaacaaag aaattgccga ggccatgcaa ctcagcgaaa agaccgtgaa aaactacctg
     3481 gctaatctct tttccaaact ccatatttcc cgacgctctc aggccgccgc cttctacgtc
     3541 cggagagcca cctcggacgg cgcgcgcacc gaatgaccct tcggtagaac ccgagcatcc
     3601 tccaagcggg gctcgacggc ctgatcttga caaacgtc
//
LOCUS       DATDNW010000382         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_266555, whole genome shotgun sequence.
ACCESSION   DATDNW010000382 DATDNW010000000
VERSION     DATDNW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266555"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..546)
                     /gene="fabZ"
                     /locus_tag="VI359_09100"
     CDS             complement(100..546)
                     /gene="fabZ"
                     /locus_tag="VI359_09100"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY6974448.1"
                     /translation="MAVMEQAEIQSLLPHRYPFLLVDRIHELDPDRRIVGMKNVTINE
                     PFFQGHFPGRPVMPGVLIIEAMAQVGGILAFKSLGQVARPVVYLTGVDNAKFRRPVVP
                     GDQIRFEIDLVKKRVPFWKMQGKAFVGTELVCEADITAMVTEEKAG"
     gene            complement(587..1126)
                     /locus_tag="VI359_09105"
     CDS             complement(587..1126)
                     /locus_tag="VI359_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY6974449.1"
                     /translation="MKGLRRGGWVLGVIGWWLVVASQAVAAETLKVAIIDQQVVIEKS
                     KAGKRALEELKSYAATRQKIINADDQELKELEQSIQDSKLSDAAKQEKQGQFQAKLEA
                     YQRRLADFNREIQQKQRETVAEYSKKVREAAQAVSQREGYTAVIDRGNDAMIRIVIYY
                     QPGLDITEQVVKEFDRQYK"
     gene            complement(1171..1707)
                     /locus_tag="VI359_09110"
     CDS             complement(1171..1707)
                     /locus_tag="VI359_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY6974450.1"
                     /translation="MTRGVAAGCLLALWLLSLAACTGAGPRIEGKVGVVDPARALNDT
                     NAGKKARDALTAFSKNRQALIDLEEKELRRMEEDFVKQASVLSPAAKKEREDQFRRRM
                     GEYQQKVTELNREVQEKQKDVLDGFRDKLELVVGKVSKRLGLQVVIDKGKGGPTIYSD
                     ETLDITGQVLEEFNREYP"
     gene            complement(1766..>2486)
                     /locus_tag="VI359_09115"
     CDS             complement(1766..>2486)
                     /locus_tag="VI359_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY6974451.1"
                     /translation="FVTGSFSLVAEDLNFQDPNNYCLSTVTANQFVCRQLGSQTTTGF
                     RTSVFRDTRDYYLDPRTGWRIGGGFDYGTPYLGGTNNFYKYYIDAIKYTPLPYDTRFS
                     LHVRYGDAEGIKDKPVPLTELFFVGGINTMRGFVFGRAGPVDPNTYALLGANKQLIFN
                     NDFIFTISSEAKLNGVIFFDYGKGFAQDESVTLNLRKAAGIEGRWISPFGPLRVAYGI
                     NLEPRTGERKGVFEFTMGQMF"
BASE COUNT          502 a          808 c          586 g          590 t
ORIGIN      
        1 atgacaaaac ggccccacgg ccacgtcggc agctaactca gccttcggat gcacaatggc
       61 actcggatga atctgcactc gctacctccc cgataatcgt tagccggctt tttcttccgt
      121 caccatggcc gtgatgtccg cttcgcacac cagttcggtg ccgacaaatg ctttcccctg
      181 catcttccaa aatggcacac gcttcttgac aaggtcgatc tcaaatcgaa tttgatcgcc
      241 gggaaccacc ggcctgcgga atttcgcgtt gtcgaccccc gtcaaataca ccaccggcct
      301 ggccacttga ccgagtgatt tgaacgccag aatcccaccg acctgagcca tcgcttcgat
      361 gatcaacact cccggcatca ccggacgccc cgggaaatgg ccttgaaaga acggctcatt
      421 gatcgtcacg ttcttcatgc ccacgatccg ccgatcagga tccagctcgt ggattcgatc
      481 gaccagcaag aacggatacc ggtgcggcaa gagcgattgg atttctgcct gttccatcac
      541 agccatcgac gatgcctcct ctttaatctc acgatctggt gttccgctac ttgtactgcc
      601 gatcaaactc cttgacgact tgctccgtaa tatcgaggcc cggctgataa taaatgacga
      661 tcctgatcat ggcgtcgttt cctcgatcga tcaccgccgt gtacccctcc cgttgcgaca
      721 cggcctgcgc cgcttcacgc acctttttcg agtactcggc gaccgtctcg cgctgcttct
      781 gctgaatctc acggttgaag tcggccaacc ggcgttgata ggcttccagc ttggcttgaa
      841 attggccctg cttctcctgc ttggcggcgt cactcagttt gctgtcttgg atggactgtt
      901 ccagctcttt caattcctga tcgtcggcat tgatgatttt ctgcctggtc gcggcgtagg
      961 acttgagttc ttccaacgcg cgcttgccgg cttttgattt ttcgatcacg acttgctgat
     1021 caatgatcgc aaccttgagc gtctcggccg cgaccgcctg tgacgcaaca acaagccacc
     1081 atccgatcac acccaggacc caacctcccc gtcgtagccc cttcatagct ccctccttct
     1141 attcacgccc ccgagccggc cgcatctcca tcacggatac tcgcggttga attcttcgag
     1201 tacctgcccc gtaatgtcga gcgtctcatc actataaatg gtcggcccgc ctttcccctt
     1261 gtcgatgacc acctgcaaac caagccgttt ggagactttc ccgacaacga gctccagctt
     1321 gtcgcggaat ccatccaaca cgtctttttg tttttcctgc acttcacggt tgagttcagt
     1381 caccttctgc tggtactcgc ccatccgccg gcgaaactgg tcctctcgtt ctttcttcgc
     1441 cgccgggctc agcacgctgg cctgcttgac gaaatcctcc tccattcggc gcagctcttt
     1501 ctcttccaga tcgatgagcg cctggcgatt tttcgaaaag gccgtcaagg catccctcgc
     1561 ctttttcccc gcattggtat cgttcagcgc ccgtgcggga tccaccacgc cgactttccc
     1621 ctcgatgcgt ggtccagccc ccgtacaagc agccaggctc aacagccaga gcgccaggag
     1681 acagccggcc gcgacccccc tggtcatcct ctcgttgttt tgccgcatgt ctctccagcc
     1741 cacgcgcgtc agctccttcg ccatcctaga acatctgtcc catcgtaaac tcgaaaaccc
     1801 ctttgcgctc cccggtacgc ggctcgagat tgatgccata ggccacgcgg agaggaccaa
     1861 agggagaaat ccatcgtcct tctatgccag ccgcttttcg caaattgagc gtgaccgact
     1921 catcttgggc aaaccctttg ccatagtcga agaaaatgac cccgttgagc ttcgcctcag
     1981 acgaaatggt gaagatgaaa tcgttattaa agatcagctg cttattcgca ccgagcagcg
     2041 cgtaggtgtt cgggtcgacc ggccccgccc gaccgaacac gaatccgcgc atggtattga
     2101 tgcctccgac gaagaagagt tcggtcagcg gaaccggctt atccttgata ccctccgcgt
     2161 ccccataccg gacatggagc gagaaccgcg tatcgtatgg gaggggagta tacttgattg
     2221 catcaatgta gtacttgtaa aaattgttcg tcccaccgag gtacggcgtg ccatagtcaa
     2281 agcctcctcc gatccgccat ccggttcgag gatcgaggta atagtctctg gtatcgcgaa
     2341 ataccgacgt gcgaaatccc gtcgtcgtct gactgcccaa ctgccggcag acgaactgat
     2401 tcgcagtaac agtactaagg cagtagttgt tcggatcctg gaagttcaga tcttccgcca
     2461 cgagactgaa actccctgtt acaaat
//
LOCUS       DATDNW010000383         6531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_333510, whole genome shotgun sequence.
ACCESSION   DATDNW010000383 DATDNW010000000
VERSION     DATDNW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333510"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VI359_09120"
     CDS             complement(<1..215)
                     /locus_tag="VI359_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974452.1"
                     /translation="MKRTLPAHDLLLAGMIAAAVLSGLLVRSLWPNQLWVNMPLHSTV
                     EALGGLAAVLMGLVLLARKAEWEDERL"
     gene            389..646
                     /locus_tag="VI359_09125"
     CDS             389..646
                     /locus_tag="VI359_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974453.1"
                     /translation="MPPKRKDPRKWGRRRPAVRARKGTSAKSGTAGLNRRLDELIERI
                     DVVLPEVTARVAALEHLLLELNLCTMDDLRGARQFVRVQEA"
     gene            1128..1298
                     /locus_tag="VI359_09130"
     CDS             1128..1298
                     /locus_tag="VI359_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974454.1"
                     /translation="MAKIQPPQLTHYDPRGLSSLLLLTWLVRTVSWVFFLCLTFWALW
                     FLWFGDRHPLDK"
     gene            complement(1389..1808)
                     /locus_tag="VI359_09135"
     CDS             complement(1389..1808)
                     /locus_tag="VI359_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974455.1"
                     /translation="MRGAKTNIWTLVLAVAFALVVSALDLLPQFENVPWYGLFVVPVI
                     WVALWSAEDDVFPIIAVAVIATSLAMIRSHISVGIWAPAPIVDRLVVASIIWLTVLLA
                     ILRKRARRTFKWITLAGRQWEPDKPANHTNQKDQTTK"
     gene            complement(1968..2486)
                     /locus_tag="VI359_09140"
     CDS             complement(1968..2486)
                     /locus_tag="VI359_09140"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY6974456.1"
                     /translation="MSVLVSREQIEQRILVIRGHRVMLDSELASMYGVTTFNLNKAVK
                     RNIDRFPEDFMFQLTAVGAMALRFQIGMSKTQGRGGRRYAPYVFTEQGVAMLSSVLKS
                     KRAIQVNIAIMRAFVKLRQLAGSHKELAAKLEQLERKVGGHDKQIRSLFEAIHQLMEP
                     PATSSRRIGFKT"
     gene            complement(2617..4017)
                     /locus_tag="VI359_09145"
     CDS             complement(2617..4017)
                     /locus_tag="VI359_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY6974457.1"
                     /translation="MTSTDQPQTDKPPSEDQATDHRPRVPLYIQVLIAVACGTALGMA
                     FGQVPYLGGLGNDDLGRLGMFVVTVLKTLAIPLVFFAIVDACLRTTLPLRQGTKLLVI
                     CFINVSVAMTVGLVIMNTWKPGVSWSGHMDELLNVVPGSTASSTVTFGGTSSDRLHPI
                     AHLVAYIPQSMLRPFSTNNVIGVVLLALVTGAILRRWQNHAAPNRGEAWALAQIVERI
                     YRALMYILGWIIRAIPFAVFAVLAYVIGRAGISVFADLWIFLVVMTLGLALHALVYYP
                     AVAWLIGKKSPKKYIGGGADAIMTAVSCNSSLATVPVTLRCLERMNVSPESARLAACV
                     GTNLNNDGITLYEAMAALFLAQALGFDLPMANQLLIVIASVMAGVGVAGIPEAGLIVL
                     PLVLSAAGLPNHIIAAAVPLILTVDWIIARARSGVNVMSDMLVAILLDRWRHRPATES
                     IALLSGSSEPNTTMSR"
     gene            complement(4136..5140)
                     /locus_tag="VI359_09150"
     CDS             complement(4136..5140)
                     /locus_tag="VI359_09150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY6974458.1"
                     /translation="MIIPAIKRVRLRISRLDRTVIMAVVLLASVFPVELFPNTPTQPK
                     GIDGQYSGKQGQVLVVKVPGEEQATEIRGTFLTRTIPFFPDPRPGGSPGYVGLLGIDM
                     QDEPGTHELAVDIQEGEQVRKLSFNVLVVKEKFHVEHLTLPKDKVDLDEKSLARWKAE
                     QEQVKQALAENSRLRLWRSDFVEPVNGKRTGIFGSVRIMNGQARSPHNGEDIGAPAGT
                     EVAATNDGIVRITVDHLLSGRGVYVDHGLGFYSMYFHLSDVLVKDGDLVRAGQIIGKV
                     GATGRATGPHLHWGVKLNGARVNPYALLDLPFKNGSAPATTVAAPDTVSPAPAVPVKD
                     "
     gene            complement(5137..>6531)
                     /gene="pafA"
                     /locus_tag="VI359_09155"
     CDS             complement(5137..>6531)
                     /gene="pafA"
                     /locus_tag="VI359_09155"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR03686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEY6974459.1"
                     /translation="MMPPGPMKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAAEER
                     LREEGLSGEIYIFKNNTDSLGNTYGCHENFLMRRDVDFWKVTEQLIPFFVTRQIYTGA
                     GKVLKVSGKPQFFVSQRAQHIHEKTSSSTTSSRSIINTRDEPHADAERYRRLHIIVGD
                     SNMSEYATYLKVGTAALVLSMIEEGFTVHGMELEDPVKAIREISRDATLKRKIKLDDG
                     RQLTAIEIQRVYLDRAQDYLAQQDHDPIHDDVFERWALMLDRLEDDPMQLVREVDWIT
                     KRHLIQSYADKKGCGWDDPRVFLLDLQYHDVKRTRGLYYLMESRGLIERIVEEDAVQR
                     AMSTPPQTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRV
                     DELVSQVSGLRFYR"
BASE COUNT         1306 a         1886 c         1994 g         1345 t
ORIGIN      
        1 tggagcctct catcttccca ctccgctttc cgcgcgagta acacgaggcc catcaggacg
       61 gccgccaacc cacctaacgc ttccacggtg gaatggaggg gcatgttcac ccatagttga
      121 ttgggccaca gggatcggac cagcaggccg gacagcacag ctgccgcgat cataccggcg
      181 aggaggagat cgtgtgccgg gagtgtgcgt ttcatttcag gctcagccct tcggtgaaca
      241 atgaacagga tgcgtcgggg acattacgac gggacggacc ggaggtcaag ccgacggctc
      301 accctggcgt cacgaccttg tacgcatgat ggtgcttgac caatgctgca aagacgcgta
      361 gaggaagcgc ttcagatagg agggacctat gccacccaag aggaaagacc cgcgcaagtg
      421 gggacgacgt cgaccggctg tgcgggccag gaagggcacg tcagccaaga gcggaacggc
      481 cggactcaat cggcggctgg atgagttgat cgagcggatt gatgtcgtac tgccggaggt
      541 gacggcccgg gttgcggcgc tggaacatct gctgctggag ctgaacctct gcacgatgga
      601 cgatctgcgc ggcgcacgac aattcgtgcg cgttcaggag gcctaggtcg cgggggctgg
      661 tctctctggt tcattgtctt tatcgaaccg aagagaccac acgaaccgga gagacgaccg
      721 acacggactt caccctgact cgcccaccct cacgcggtct ccgtgcctga tcgttccgcc
      781 ttgcacgacc gctgcataaa cgcccacatg gccctgattg agctggactg cggtgcgcaa
      841 gatgccgctc tccttcggga ggtctccctg cgcgagtggg tgtttggctc gggagctttt
      901 ggcatagggg gaagagcgac aggaaggcga agggtgtgaa atatgaggag tcaggatggt
      961 cacagcgacg ccctctgccc cttttgaggt agttcgtttg agtctggcgc gacccctggc
     1021 cagcttctat cttatagatg gaggccatga ccatacgtat atctcggtag gcatggaggg
     1081 gaagaccgat catggcctgg tgaatgcgaa tgccggaggg actggacatg gcgaagatac
     1141 agcctcctca actcactcac tacgatcccc ggggtctcag ctctctgttg ctgctcacct
     1201 ggctcgttcg tacggtctct tgggtgtttt tcctgtgttt gactttctgg gcgctctggt
     1261 ttctttggtt cggtgatcga catccactcg acaagtagac acgcccatca caccagccag
     1321 acacaattgt cgtcatgcca tcgatgagcc ggccgagtgg ggaactggct tgtctggtcg
     1381 atccggtgtt atttggttgt ctggtctttt tggtttgtgt ggttcgcggg tttgtccggt
     1441 tcccactgtc tgccggcgag ggtgatccat ttaaacgtgc ggcgcgcgcg ttttcggaga
     1501 atggccagga ggacggtgag ccaaatgatg ctcgcgacga cgaggcggtc gacgatcgga
     1561 gccggagccc agatgccgac ggagatgtgg cttcggatca tcgcgaggct cgtggcgatg
     1621 accgccacgg cgatgatggg aaagacgtcg tcctccgcgg accacagggc gacccagatg
     1681 accggaacga cgaacaaccc ataccacggc acgttttcaa attgaggcag caggtccagc
     1741 gctgacacga cgagcgcgaa cgcgaccgcg aggaccaggg tccatatgtt ggttttcgct
     1801 cctctcacgc tctcaacccc gtttcataac tccccatcca gcttaatggg ccacatgcgg
     1861 ctgtacaacc ggaatttctc gctctcaatc ggtggtttac tcgggcggtg acgggctgag
     1921 ggctcggatg acgtgagggg tcaggcgtga gtggtgcggt tcttgtctca ggtcttaaat
     1981 ccaatgcggc gggagcttgt ggcgggtggc tccatcagct gatggatggc ttcgaagagg
     2041 gatcgaatct gcttatcgtg tcccccgact ttccgttcga gttgctctaa cttcgcagcc
     2101 aactctttat ggcttccggc aagttggcgt agcttcacga atgctcgcat gatcgcgatg
     2161 ttaacttgaa ttgcccgctt gctcttcagc acactcgaaa gcatcgccac accttgttct
     2221 gtgaaaacgt acggtgcata tcgccgtcct ccccgtcctt gagtttttga cattccaatt
     2281 tggaatctca acgccatggc tcctacggct gtcaattgaa acataaagtc ttctgggaac
     2341 cgatcgatgt ttcgcttcac ggccttattg aggttgaagg ttgtgactcc atacatagaa
     2401 gccaattccg aatccaacat gacccgatga ccccggatca cgaggattct ctgttcaatt
     2461 tgttcacgag acaccagcac actcatactc gctcctccgc atgagacgct catccgtaag
     2521 gttgcatgga gataccgttt tcatggaacg aattcaacgg atgaagagtc caaatggaag
     2581 tattaacaag gggttggcgc gacgggcaag acaggttcag cgggacatcg tggtgttggg
     2641 ttccgaggat ccggatagca atgcaataga ttcggtggct ggtctatgtc gccaccggtc
     2701 gagcaggatg gccaccagca tgtcgctcat gacgttcacg cccgaccggg cccgggcgat
     2761 gatccagtcg accgtcaaga tcagcgggac ggcggccgcg atgatatggt tgggcaagcc
     2821 ggcggcggag agcacgagcg gtaagacgat caggccggct tccggaatcc ctgccacccc
     2881 caccccagcc ataaccgacg cgatgacgat caggagctga ttcgccatcg gcagatcgaa
     2941 cccgagcgcc tgggccagaa agagcgcggc catcgcttca tagagcgtga tgccgtcgtt
     3001 gttcaggttc gtcccgacac aggccgcgag gcgcgcggat tcgggtgaga cgttcatccg
     3061 ttcgagacag cggagcgtga ctgggacggt ggcgagactg ctgttgcacg agacggcggt
     3121 catgatcgca tcggcgcccc ctccgatgta cttcttgggt gacttcttcc cgatcagcca
     3181 ggccacggcg gggtagtaga cgagcgcgtg cagcgcgagc cccagcgtca tgacgaccag
     3241 aaaaatccag aggtcggcga acacgctaat ccctgcccgt ccgatgacat aggccagcac
     3301 ggcgaacacg gcgaacggga tcgcccggat aatccagccg aggatgtaca tgagtgctcg
     3361 atagatccgt tcgacgattt gcgcaagcgc ccaggcttcg cctctgttcg gggccgcatg
     3421 gttctgccag cggcgcagga tggcaccggt aaccagcgcg agcaacacca ctccgatcac
     3481 gttgttggtc gagaagggac gcaacatgct ctgcggaata taggccacca agtgggcaat
     3541 cgggtgcagc cgatccgacg aggtgccccc gaacgtgact gttgacgacg cagtcgagcc
     3601 aggcacgacg ttcagcagct cgtccatgtg accggaccac gacacacccg gcttccaggt
     3661 attcatgatc acaagaccga ccgtcatcgc gacggagacg ttgatgaagc agatcaccag
     3721 cagtttggtg ccctggcgca atgggagagt ggttcgaaga cacgcatcca cgatcgcgaa
     3781 gaagacgagg ggaatggcga gggtcttgag caccgtgacg acgaacatcc cgagccggcc
     3841 gaggtcatcg ttgcccagac cgcccaggta gggcacctgg ccgaaagcca tcccgagtgc
     3901 cgtcccgcag gccacggcga tcagtacctg gatgtagagc ggcacccgcg gacggtggtc
     3961 cgtggcctgg tcttctgatg gtggcttgtc cgtctgcggc tggtcggtgc tggtcaccgg
     4021 ggatctttct gatcgagcat catagcgacg gggatgaaga ataccgtttt tacagaaaga
     4081 aatcagccta cccgaaggga cggggttcga gcatgatctg ctgaacggac gtaggctaat
     4141 ctttcacggg aacggctggt gcgggagaca ctgtgtctgg agcggcgact gtggtcgcgg
     4201 gggcggagcc gttcttgaac ggaagatcga gcagcgcata gggattcacg cgggcgccgt
     4261 tcaatttgac gccccaatgg agatgcggtc cggtggcgcg gccggttgcg ccgaccttgc
     4321 cgatgatctg gccggccctc accagatctc cgtctttgac gagaacatca gaaaggtgga
     4381 agtacatcga gtagaagccc aggccatggt ccacgtagac gcctcggccg gacaagagat
     4441 gatcgacggt gatccggacg atcccgtcgt tcgtggccgc cacctcggtg cccgccggcg
     4501 cgccgatgtc ttcgccgtta tggggactcc tggcttggcc gttcatgatc cggacactgc
     4561 cgaagatgcc ggtgcgcttc ccgttgaccg gttcgacgaa gtcgctccgc cacaagcgga
     4621 gacgtgaatt ttccgccagg gcttgcttca cctgttcctg ctcggctttc cagcgcgcta
     4681 ggctcttttc atcgaggtcg accttgtctt tgggcagcgt gagatgctcg acgtgaaact
     4741 tttctttgac gaccagcacg ttgaagctca gcttgcgaac ctgctcgccc tcctggatgt
     4801 cgaccgccaa ttcatgggtt cccggctcat cctgcatgtc gatcccgagc aggcccacat
     4861 agccaggaga tcctcccggt ctgggatcgg ggaaaaatgg aatcgtccgc gtcaggaacg
     4921 ttcctcggat ctcggtggcc tgttcttcac cgggcacctt gaccacgagc acctggccct
     4981 gtttgccgct gtactgcccg tcgatgcctt tgggctgagt cggcgtattc ggaaacagct
     5041 cgacgggaaa cacactggcc agcagcacga ccgccatgat caccgtccgg tcgagtcgcg
     5101 agatgcgcag acggaccctt ttgatcgccg gaataatcat cggtaaaacc gtagtcccga
     5161 aacctgcgac acgagttcat ccacccgccg gttcacggcg ctgaagggat ccatacagag
     5221 aatcgtttcc tcccaatatc cgttcagctt gagataggtc caatccaccg tgtaggagcg
     5281 attcttcgcc cgggcaaacc ggatgaaatc gccgcgcacc ttggccctgg tcgtctgcgg
     5341 cggcgtcgac atggcccgct gcacggcatc ctcttcgacg attcgctcga tgagcccgcg
     5401 cgattccatc agataataca atccgcgtgt ccgtttcacg tcgtggtact gcagatcgag
     5461 caggaagacc cggggatcgt cccagccgca tcccttcttg tccgcatacg actgaatgag
     5521 gtgcctcttg gtaatccagt ccacctcccg caccagctgc atcggatcgt cttccaatcg
     5581 atcgagcatc aaggcccagc gctcaaacac atcgtcatgg atcggatcgt ggtcctgctg
     5641 agccagatag tcctgcgccc gatccaggta gacgcgctga atctcgattg cggtcagctg
     5701 ccggccgtcg tccagcttga tcttgcgctt gagtgtggcg tcgcgggaaa tctcgcggat
     5761 cgccttgacg ggatcttcca gctccatccc gtgcacggtg aagccctctt cgatcatgga
     5821 gagcaccagc gcggcggttc ccaccttcag ataggtggca tattcggaca tgttggaatc
     5881 gccgacgatg atgtgcaggc ggcgataccg ttcggcatcg gcatgcggct catccctggt
     5941 attgatgatg ctgcgcgagg aggtcgtcga cgacgaggtc ttttcgtgaa tgtgctgggc
     6001 gcgttgcgag acgaaaaact gcggcttgcc ggagaccttc agcacttttc ccgctccggt
     6061 gtagatttgc ctggtcacga aaaacggaat cagctgttcc gtgaccttcc agaaatcgac
     6121 gtcgcgccgc atcaggaaat tctcgtggca gccgtaggtg ttgccgagcg aatccgtatt
     6181 gtttttgaag atgtaaatct ctcccgacag gccctcctcg cgcagccgtt cctccgccgc
     6241 cggcaaacag gcttcgagca gccgctctcc ggccttgtca tgcacgacga gatcgagaat
     6301 gttgtcgcat tccggcgtcg aatattccgg atggcagccg gtatcctggt agaaccgggc
     6361 gccgttgacg agaaacgcgt tggagggcca gctgttggga atgaggcctt cgaagatata
     6421 gcccagcacc ttctccatcg gcagatagat gcggccgttc ggggagaaga tgaggccgta
     6481 ctcgttctcc aggccgaaga tccgctgttt catggggccg gggggcatca t
//
LOCUS       DATDNW010000384         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_129784, whole genome shotgun sequence.
ACCESSION   DATDNW010000384 DATDNW010000000
VERSION     DATDNW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129784"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(584..733)
                     /locus_tag="VI359_09160"
     CDS             complement(584..733)
                     /locus_tag="VI359_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974460.1"
                     /translation="MKRLKRADYPVCVIGWENSQDHEIEASVASVEEGLIHFTSPALT
                     HHFIK"
     gene            982..1578
                     /locus_tag="VI359_09165"
     CDS             982..1578
                     /locus_tag="VI359_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY6974461.1"
                     /translation="MNRRGFWLGLAVGTISACLLGQWVFSAASKETDPPKGISPEMVA
                     DYIHSVIQADRTFYSSEIVERLQTRGIVSASEHWKETGDLPLPAQFVLETGRLVAKQP
                     NGIRFRLISSWAINKRNSPTTEFERTGLTKILVNSDRPYTGVTTEGGARVFQALYADK
                     ALSQRCADCHNVHSRSPKRDFKVGDVMGGILITVPLPK"
BASE COUNT          646 a          655 c          669 g          562 t
ORIGIN      
        1 gtacaaaaac tcctgctcca atatggattt gattccgtgc catcgaatgg gtgacttact
       61 ctctggcctt cttagttatc tagattactc aggtcttgcc gccttagcgc tttttcggcg
      121 ccgtgccgat tggacgacgc cgacccccac ccaatgattt tccgtcacga ccaaccacag
      181 tctccttctg ctccagctga ttccccgtct agagattggc gagtgagcca tatcaaactt
      241 ccggaatttg cttggagtat tgtggagccg gcgacccgga ttgaacgggc gacctgcggt
      301 gtgatagtga atgattcggt caaacctgat cgggcagaat tgtcctgcca caatttaccc
      361 tcagccgcac cagaagaagg gtgggtcaaa aactctagaa gcgatcccct cgtagcacct
      421 cgattttaat aggagatacg cgtatccttc cagaagtcca aaaaacaaat gaattagaag
      481 gaagcatggt tcacacatga aaacgctgat gattgtggct cgggactcta ggatgcatga
      541 gttggaggag gtcttgcacg ataatggcgt caatgcgtac tcactacttg ataaagtgat
      601 gggtaagggc aggactggta aagtgtatga gtccctcttc cacactggct acactggctt
      661 caatctcatg atcctggctg ttctcccatc cgatcacgca gacagggtag tcagcgcgct
      721 taaggcgttt cacgcagcac gagtgaaggg cgcacacggg gagcccattg cctttaagct
      781 gttttctttc ccttgtgaag aactcatata gcgccatggg ctacaaccgc ccagtgtctt
      841 catagcaagg gtttctcagc cttaagagtg gttcaatagg aatagctcag aaactgttcg
      901 gcattattgg tttgaccgcc cggctatcgg agccaatggg cgaatcaagc cacaggcaga
      961 aaaagaactg gaggccgcat catgaacaga agaggctttt ggttgggact cgctgttgga
     1021 accatctccg cttgcctact cggtcagtgg gtgttctccg ccgcgagcaa ggagacagat
     1081 ccgcccaagg gcatatcccc cgaaatggtc gccgattata ttcatagtgt gatccaggcg
     1141 gatcgaacgt tttactcgag cgagattgtg gagcggctgc agacgcgagg cattgtctca
     1201 gcctcggaac actggaaaga gaccggagat ctccctttac ccgcccaatt tgtactcgaa
     1261 acggggcggc tcgtagctaa acaaccaaat gggattcgat tccggctgat cagcagttgg
     1321 gcgataaata aaaggaatag cccaacgaca gagttcgagc ggaccgggct tacgaagatc
     1381 ctcgtgaatt ccgatcgtcc ttatacaggc gttacaaccg agggcggggc tcgcgtcttc
     1441 caagcactct atgccgataa ggccctgtct cagaggtgcg ccgactgtca caacgtgcac
     1501 tccagaagcc caaagagaga tttcaaggtg ggagacgtaa tgggtgggat tcttataacg
     1561 gttccactcc ccaaataaga aaggccaact gaaatgagct gttcaggcgg cacttgttgc
     1621 acccgcagac cgtgcgcagc ctgatacgtt gacggttggg gcaagcagtg gtgtggacct
     1681 gtcggaacga atcccatact gccgctgaaa agtctttcga tttcatcatg agtccccgca
     1741 tcccgaaact cctgctcaag tatggatttg actccgtgtc gtcgaatggg tgagcgagcc
     1801 cgaatagcgg atcgactcgc cattatagac tcagcgaagc ggtaggaaag atctgcagag
     1861 cctgcttatg cgcggggttc cgattcgaag ctcaatagaa ggcgttcggg aaagtagtgc
     1921 tcagcgaggt ggcgacgccc acccgattcc attccaaaag ctctatgaca agactgatga
     1981 cgacgcgaac cgcttagaca gcatggagct ttgtaagaaa tcctgaacca ctcctggtca
     2041 gccgcagcaa acctgaccga ctcaggcggt ctacttcgat aaatacctga gaccaattca
     2101 tttgcggcaa gtattgtgtt aactcgtcca ggccgcaccc aggatgagtc ctcacgacct
     2161 cgagaatctg attagcagtt gcgctttgtc ccaccatagg cgcctcccgg ttcgaaacag
     2221 ataccttcgg cccgcaacat gatcggaatt ccaggactga ctcagggact acagacgagc
     2281 aagttgtatt ccgctcgaag ggcgagagag gattaccgac atcatgcagc agaagatcaa
     2341 acaatagaag aggataggag tcgcatctcc aacaaacgtg aagagtaacc gcctcccttt
     2401 atcgactttt tgcatttttg taccgtgcgt cgtgagcgga accgctcgaa taggaatcga
     2461 cgagaaacct accgagagcc atcaatccag cagatcttca tgggggatga gaacagggta
     2521 agactgccag aa
//
LOCUS       DATDNW010000385         3857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_402310, whole genome shotgun sequence.
ACCESSION   DATDNW010000385 DATDNW010000000
VERSION     DATDNW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402310"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VI359_09170"
     CDS             complement(<1..1466)
                     /locus_tag="VI359_09170"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY6974462.1"
                     /translation="MTVAQLLASVREQVEVLERRGNLDLTVTSMTDDSRTVSKGSLFV
                     AVKGERVDGHDFVENAKQAGAAAVVAQRSVETGSLPYVRVSDSRKALGWLGSRFHGDP
                     SARLTMVGITGTNGKTTTTYLCRALLEGIGRRVGLIGTVAYQIGSETIPASHTTPGAL
                     ELQQLLAKMAESGLDTAVMEVSSHALALDRTDGCEYDVAVFTNLTQDHLDFHSDMEDY
                     FKAKLRLFTELAHGHKTGKRAIINLDDPYGARVRAACPVPVWGYGLSSQAELKAEGVR
                     LSPNGTTFTAATPAGSFPVESRLVGEHNVYNLLGAIGVAVHAGATPTQVQEAVAGVTN
                     VPGRFERVSAGQDFTVVVDYAHTPDALVRLLTAAQAVKTHRIITVFGCGGDRDRGKRP
                     KMGRAAVEQSDVVVLTSDNPRTEDPMAILHEVEVGVREALQRRPHVAYRMIPDRREAI
                     GSAISEARSGDMVLIAGKGHEDYQIIGTKKFHFDDREVA"
     gene            complement(1474..3186)
                     /locus_tag="VI359_09175"
     CDS             complement(1474..3186)
                     /locus_tag="VI359_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY6974463.1"
                     /translation="MGPSRPRRYVVLILLLCGFGLILFRLVTLQVLQAAELTAKADRQ
                     HQKSVTFEGARGAITDRHGKVLAMNMEVPSVFGVPTALENPLRVARHLAPVLHIRSDE
                     IEKKLRQDRSFVWVARKVLPEQGRQLEQMSLDGIGVVMEGRRFYPKGPLLSHVLGFAG
                     IDGEGLEGLERRYEPHLHGEKRITILQRDALGRTVFPKGLAEQPPAPGHALTLTIDEV
                     VQYIAEKELEDAVNHAHAKSGTIIVLDPQSGAILAMAVSPRFDPNAVAALTPDRWRNR
                     ALTDTYEPGSTMKMVVAAAALEEHLMTPGTMVFGENGRMLVANTTIHDHEKLGWMTFA
                     QMIQKSSNIGAAKTGMILGEQRLYRYLQGFGFGQKTDVDLPGEVSGLLKAPKEWGRRS
                     LASISMGQEIGVTPIQMVTAVATLANGGVMMKPYLVSEVRDEKGHLLKEFLPQVKRRV
                     LSPETARTMTTILEGVVTNGTGTKAAIPGYRVAGKTGTAQKIDSRTGTYSATQFVGSF
                     VGYVPADAPRLAMIVVIDEPQGEAWGGTVAAPVFRRVGEQVLNYLGVSPNDQIKVAMA
                     AHGR"
     gene            complement(3189..3524)
                     /locus_tag="VI359_09180"
     CDS             complement(3189..3524)
                     /locus_tag="VI359_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY6974464.1"
                     /translation="MKMLTILAGLILVFVFVWERVDTIRLGYQVERSKAQKLLLEREH
                     DQLQVRFSSLTAPERIAKVATERLGMVQPQQGQVVTVHMKPEGVPAPSPSPYEVRVAK
                     NDSPKRLSE"
     gene            complement(3521..>3857)
                     /gene="mraW"
                     /locus_tag="VI359_09185"
     CDS             complement(3521..>3857)
                     /gene="mraW"
                     /locus_tag="VI359_09185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY6974465.1"
                     /translation="HYRHGRIHCATRTFQALRIAVNQELECLEPSLRDAVDILAPGGR
                     LCVISFHSLEDRIVKQTFRALSFGAEAPITVLTKKPQLPQEDEVREHPRSRSAKLRVA
                     QRREREGHS"
BASE COUNT          766 a         1241 c         1048 g          802 t
ORIGIN      
        1 gcgacttcgc gatcgtcgaa atgaaacttc ttcgtgccga tgatctgata gtcttcgtgc
       61 cctttccccg caatgagcac catgtcgccg gatcgggctt cgctgatggc ggatccgatg
      121 gcctcgcgcc ggtccgggat catgcgatac gccacatgcg gccgccgttg cagcgcctcg
      181 cgcacaccga cctctacttc gtggagaatg gccatcggat cctccgtccg agggttgtca
      241 gacgtcagca cgacgacatc gctctgctcc acggcagccc ggcccatctt ggggcgcttg
      301 ccgcgatcgc ggtccccgcc gcagccaaag acggtgatga tgcgatgagt tttcacagcc
      361 tgtgcggccg tcagcaacct gaccaacgca tctggcgtat gggcgtaatc cacgacgacc
      421 gtgaagtcct ggccggcaga cacacgttcg aatcggccgg gcacattcgt gaccccggcc
      481 acagcctcct gcacttgtgt tggtgtcgcg ccggcatgca ccgccacgcc aatggcgcca
      541 agcaagttgt agacgttgtg ctcgccgacc aaccgacttt cgaccggaaa cgatcccgcg
      601 ggagtcgcag ccgtgaatgt ggttccgttc ggagacaagc gcactccttc ggctttcaac
      661 tcggcttgac tggagaggcc atagccccac accggaacag gacaggcggc gcggacccga
      721 gcaccgtaag gatcatcgag attgatgatc gcccgcttcc cggttttgtg tccgtgagcg
      781 agttcggtaa agagccgcaa cttcgccttg aagtagtcct ccatatcgga atggaaatcg
      841 agatggtcct gcgtcaaatt cgtgaacacc gcaacgtcgt actcacaacc atccgtccga
      901 tcgagtgcca gtgcatggga ggacacctcc atgaccgccg tgtcgagtcc gctctctgcc
      961 atcttcgcca gcagctgctg gagctccaac gcacccggcg ttgtgtggga agccggaatt
     1021 gtttcgctcc cgatttgata ggccacggtt ccgatcaatc ccacccgccg gccgattcct
     1081 tccaacagcg ccctacagag atacgtcgtc gtcgtcttgc cattcgtacc cgtgatcccc
     1141 accatggtga gacgggcgga cgggtcgccg tggaaccggc tacccagcca cccgagcgct
     1201 ttcctcgagt ctgagactcg gacatacggt agcgatccgg tctcaacgga tcgttgagcc
     1261 accacggccg cggcgccggc ctgttttgcg ttctcgacaa agtcgtgacc gtccacccgt
     1321 tcacctttga ccgccacaaa gaggcttccc tttgacaccg tccgcgaatc atccgtcatg
     1381 ctcgtcacgg taagatcgag atttcctcgt cgctccagca cctcaacttg ctcgcgcacc
     1441 gaagccagaa gctgggcgac cgtcatcgtg tccctaccgc ccgtgcgccg ccatggcgac
     1501 cttgatctgg tcgttcgggg acacacccag ataattcagc acttgctcgc ctacccgtcg
     1561 aaagaccggc gcggcgacgg tgccacccca ggcttctccc tgcggctcgt cgatcacgac
     1621 gatcatcgcc aatcgcggcg cgtcggccgg gacgtagccg acgaacgacc cgacaaattg
     1681 cgtagccgaa taggttccgg tcctcgaatc gattttttga gccgtgccag ttttgccagc
     1741 gacccgatac ccgggaatgg cggccttcgt accagttccg ttcgtcacga ctccttccaa
     1801 gatagttgtc atcgtgcgcg ccgtttccgg ggacagaacc cgtcgtttca cctgaggcaa
     1861 aaattccttc agcaaatggc ccttctcatc gcgtacctcg gagaccagat acggcttcat
     1921 catgacaccg ccgttggcga gggtggcgac cgcggtcacc atttggatcg gggtaacacc
     1981 aatttcctgc cccatggaga tcgaggcgag tgagcgtcgc ccccattctt tgggagcctt
     2041 caataatcca ctgacctcgc ccggcagatc gacgtccgtc ttctgaccaa atccaaaccc
     2101 ctggaggtaa cggtagaggc gctgctctcc caggatcatg ccggtttttg ccgcaccgat
     2161 gttgctcgac ttctggatca tctgcgcaaa tgtcatccag ccaagttttt catggtcgtg
     2221 aatcgtggtg ttggcaacca acatccgccc gttttcaccg aagaccatcg tcccgggagt
     2281 catgaggtgc tcttcaagcg ccgccgccgc cacaaccatc ttcatcgttg atcccggctc
     2341 atacgtatcc gtcaaagctc gattgcgcca acggtcaggc gtcagcgcgg ctaccgcgtt
     2401 cgggtcgaac cgtgggctaa cggccatcgc cagaatggct cccgactgag ggtccagtac
     2461 aataatggtg ccagacttgg catgagcatg attgaccgcg tcttccaatt ccttctccgc
     2521 tatatattga accacttcgt cgatggtcag tgtgagggca tggccaggcg cgggaggttg
     2581 ttccgccaaa cccttcggga agacggtccg gcccaaggcg tcacgttgaa gaatcgtgat
     2641 ccgtttttct ccatgcaggt gcggctcata gcgccgctct aatccttcga gtccctctcc
     2701 gtctatccca gcaaatccaa gcacatgtga gagcaatggc cccttgggat aaaaccggcg
     2761 cccttccatc acaacaccaa taccgtccag agacatctgc tccaactggc gcccctgctc
     2821 cggcagaacc ttgcgcgcta cccaaacgaa acttcggtct tgccgcagtt ttttctcgat
     2881 ctcgtcgctt cggatgtgga gtaccggcgc aaggtggcgg gcaacgcgca gaggattctc
     2941 gagggcggta ggtacgccga agaccgaagg cacttccata ttcatggcca aaaccttgcc
     3001 gtgccgatcc gtgatcgcgc ctctcgcacc ttcgaacgtc acactctttt gatgttgccg
     3061 atcggctttc gccgtcagtt ccgccgcctg cagcacttgc agcgtcacca aacgaaacag
     3121 aatgaggccg aacccgcaga gcaggaggat caagaccaca taccgcctcg ggcgagaagg
     3181 gcccatgatt actcgctcaa cctcttgggc gaatcattct tcgcgactcg gacctcatat
     3241 ggtgaagggg aaggtgcagg tactccctct ggcttcatat gcaccgtcac cacctgtccc
     3301 tgctgaggct ggaccatgcc cagacgttcg gtcgccacct tcgcaattcg ctctggtgcc
     3361 gtgagtgagg aaaaccgaac ttgcagttga tcatgttctc gctccaacaa cagcttctga
     3421 gctttcgatc gttcgacttg gtaaccgagg cgaatggtat caactcgctc ccagacgaac
     3481 acgaagacca gaatcaaccc ggccaggatc gtcaacattt tcatgagtga ccctccctct
     3541 ctcgccgttg agcgacacgt aacttggcac tgcgggaccg gggatgctcg cgaacttcat
     3601 cttcctgcgg gagctgcggc tttttcgtga gcacggtgat cggcgcctcg gcgccgaagg
     3661 acagggcccg gaacgtttgc ttgactattc ggtcctcgag agaatggaat gaaatcacac
     3721 agaggcggcc gcctggagcc aagatgtcca cggcgtcgcg caacgacggt tcgagacatt
     3781 ccagctcttg attgacggca atccgaagtg cttgaaacgt tcgcgtggcg caatggatgc
     3841 gaccatgccg atagtgc
//
LOCUS       DATDNW010000386         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_530351, whole genome shotgun sequence.
ACCESSION   DATDNW010000386 DATDNW010000000
VERSION     DATDNW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530351"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="accD"
                     /locus_tag="VI359_09190"
     CDS             <1..776
                     /gene="accD"
                     /locus_tag="VI359_09190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY6974466.1"
                     /translation="EGMWLKCNHCREIVYRKEVERNNKVCPKCEYHFPISVMERIALL
                     VDLGTFKEWDVELGPQDPLNFHDTKSYRDRVKAQQEKTGRKDAMVIGEGHINGRRVVL
                     CVFDFGFMGGSMGSVVGEKICRAIDRALDARLPVILVTASGGARMQEGILSLMQMAKT
                     SAAVAKLGEAKLPLICLLADPTFGGVTASIAMLGDVIIAEPKALIGFAGPRVIEQTIK
                     QQLPDQFQRAEFLLEHGMIDMIVERKQLKETVSTLVSHF"
     gene            800..2095
                     /locus_tag="VI359_09195"
     CDS             800..2095
                     /locus_tag="VI359_09195"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY6974467.1"
                     /translation="MNYSAAIAYLYSLQKHGIKLGLETMATLMARLDSPHGQFRTLHI
                     AGTNGKGSTAAMTAAVLQAAGFRVGLYTSPHLVDFRERIRVDGKMIDEEHVSQLTQQI
                     RDACGHDLSPTFFESTTAMAFQYFADASVDVAVVEVGLGGRFDATNVVQPVACAITTI
                     ALDHQEYLGPTLSSIAAEKAGIIKPRIPVIVGRLDAEAEETIERIALEQQAPMYQLNR
                     EFQTIGASTSGFDYRGVSGRRDGLACPLAGRHQLDNAACALALLEAAAAEGLLVPEEV
                     VREGLRSVKWEGRLEIVERSPTIVLDGAHNPAAASVLADSLAEMMRERPRGRLILVFG
                     MMRDKNHRGFIMPLRQLVGEVVLTQADLPRSATVSELRVSLRDLVPFLHEAPVPVDAL
                     ALAKQLAGPDDVICVTGSLMLVGDVTAIYRGCGLSPIRG"
     gene            2097..>2926
                     /locus_tag="VI359_09200"
     CDS             2097..>2926
                     /locus_tag="VI359_09200"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEY6974468.1"
                     /translation="MAGVLVRMRGYLLGLVIAGLLIPTPGLAERTQVAVGSPSASGPL
                     PLDVRAERIDYLQNQEVYEADGAVVVDQGALHLTADHITIQALPGILIATGHVHLTDP
                     KADLVSDRLELNVNTEAGVVTNGQLHLPFSNSFVTGRVLQRFSEDHYRVKEGTFTNCD
                     AQQGEVPAWRFQFKDLDLEAGDNVAFTGVWLCVMDVPIVPMPTMTYPLSNRRSGFLIP
                     QILYDNRFGFNAQGSYYWAINPSQDLTVAPYYYSKLGYGSDVTYRYMLDRRSKGQWDV
                     "
BASE COUNT          621 a          756 c          882 g          667 t
ORIGIN      
        1 ctgaaggcat gtggctgaag tgcaaccact gtcgggagat cgtctatcgc aaagaagtag
       61 agcggaacaa taaggtctgt ccgaagtgtg agtatcactt tccgatttcg gtgatggaac
      121 ggattgccct gttagtcgac ctcgggacgt ttaaggagtg ggatgtggaa cttggtccgc
      181 aggacccgct caattttcac gatacgaaat cctacaggga tcgtgtgaaa gcccagcaag
      241 agaaaaccgg tcggaaagat gcgatggtca tcggtgaagg ccacatcaat ggccgccgtg
      301 tcgtgttgtg cgtgttcgat ttcgggttca tgggcgggag catgggatca gtcgtcggcg
      361 agaagatttg tcgcgccata gaccgtgcac tcgacgccag actgccggtg attctggtga
      421 cggcctcagg tggagctcgc atgcaagaag gaatcttgtc gttgatgcaa atggcaaaaa
      481 cttccgcggc tgtggcaaaa ctcggcgagg ccaagttgcc cctcatttgt ttgttggccg
      541 atcccacgtt cggtggagta acggcgagca ttgcgatgtt gggcgacgtg attattgccg
      601 agccgaaagc attgatcgga tttgccggtc ctcgggtcat cgagcagacg atcaaacagc
      661 agttgccgga tcagttccaa cgtgccgagt ttctgctgga acatggcatg attgatatga
      721 tcgtcgagcg gaagcagctg aaagaaaccg tcagcaccct cgtcagtcat ttctagttcc
      781 tcgttcctct tcacgcacca tgaattattc ggctgccatc gcctacctct atagtttgca
      841 gaagcacggt atcaagctgg ggcttgagac gatggccacg ctgatggcgc gactcgattc
      901 gccgcacggg cagtttcgaa cgttgcatat tgcaggcaca aacgggaaag ggtcgactgc
      961 agccatgact gcggcagtct tgcaggctgc tggattccgg gtcgggctct acacctctcc
     1021 ccatcttgtg gactttcgag aacgaattcg tgtggacggg aagatgatcg atgaagagca
     1081 tgtctcgcag ttaacgcagc agattcgaga tgcgtgcgga catgatctct ccccaacctt
     1141 tttcgaatcg acgacggcaa tggccttcca atactttgcg gacgcttcag ttgatgtggc
     1201 agtcgtggaa gtcgggctcg gtggccggtt tgacgccacg aatgtcgtac agccggttgc
     1261 gtgcgccatt accacgatcg cactggatca ccaggaatat ctcggtccga ctctctcatc
     1321 aatcgcagcg gagaaggccg gcatcattaa accgagaatc ccggtcatcg tggggcggct
     1381 agacgcagaa gccgaggaga ccatcgaacg gatcgctttg gaacagcagg ctccgatgta
     1441 tcaactgaac cgtgagtttc agaccatcgg ggcatcgacc agcgggtttg actatcgggg
     1501 tgtcagcggt cgtcgagacg gacttgcctg cccattagca gggcggcacc aactcgataa
     1561 tgccgcctgt gcgctggccc tcctggaggc ggctgctgcg gaaggattgc tcgttccgga
     1621 agaggtagtt cgagagggct tacgttccgt caaatgggag gggcgccttg aaattgtgga
     1681 gcgatctcca accatcgtgc tggatggagc ccacaatccc gcggcagcgt ctgtcttggc
     1741 ggactcgttg gccgagatga tgcgggagag gccgagggga cggctcattc tggtgttcgg
     1801 gatgatgcgg gataagaacc atcgaggctt catcatgcca ctccgacagc tcgtcggaga
     1861 agttgtgctg acccaagccg atcttcctcg ctccgcaacc gtgtctgaac tgcgtgtctc
     1921 gttaagggat ttggtgccgt tcctccatga ggcaccggta ccggtcgatg cgctcgcatt
     1981 ggcaaaacag ttggcgggcc cggacgatgt gatctgtgtg acgggttcat tgatgctcgt
     2041 gggagatgta acggcgatct accgcggttg tgggctttca ccgatccggg gttgagatgg
     2101 ccggtgtcct cgttcggatg cggggctatc ttctgggcct agtgatcgcg gggttgctta
     2161 tcccaactcc cgggttagcc gagcgcacgc aggttgctgt agggtctccc tccgcatctg
     2221 gaccgctgcc actggacgtt cgcgcagagc gcatcgatta tctgcaaaac caagaagtct
     2281 atgaagccga cggggccgtc gttgtcgatc aaggcgcgct ccatctgacg gctgatcaca
     2341 ttacaattca ggcccttcca ggcatcctga ttgcgacagg tcatgtccac ctcaccgacc
     2401 ccaaggcaga tctcgtttcc gatcgactgg aactgaatgt gaacactgag gcaggagttg
     2461 tcacgaatgg ccaactgcac cttcccttct caaattcgtt cgtgaccggc cgcgtcctcc
     2521 agcgcttttc cgaagaccat tatcgggtga aagaagggac gtttaccaac tgcgatgcgc
     2581 agcaaggaga ggtgccggca tggcgattcc agtttaaaga cctcgatctg gaagctggtg
     2641 acaacgtggc ctttaccggg gtgtggctgt gtgtgatgga cgttcccatc gttccgatgc
     2701 ccacgatgac atacccactt tccaatcgac gcagcggctt cttgattcct caaatcctat
     2761 atgacaatcg gtttgggttt aatgcgcaag gtagctatta ctgggcgatt aatccaagcc
     2821 aagacttgac cgtcgcaccc tactattact cgaagctcgg gtatgggagt gatgtgacgt
     2881 atcggtatat gttggatcgg cgatcaaaag ggcaatggga tgtgaa
//
LOCUS       DATDNW010000387         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_130114, whole genome shotgun sequence.
ACCESSION   DATDNW010000387 DATDNW010000000
VERSION     DATDNW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130114"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..990
                     /locus_tag="VI359_09205"
     CDS             10..990
                     /locus_tag="VI359_09205"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEY6974469.1"
                     /translation="MHGQNICVTASVGLAVAPDNGVTADELMRHADIALYEAKNQGRD
                     RSVLFSAEMAERVEHRRSIEMELRTALEANQLRLHYQPIIACGTGAIVGVEALLRWFH
                     PVHGEISPVEFIPIAENSGLLPSLGEWVLDQAMMDSKRWPDLEVAVNLSPVQFRHVDL
                     TSALRTLITKHNVSPNHFVLEITEGVLLEASDHTNSILEAIRAMGFKTALDDFGTRYS
                     SLAYLCNFNFDKIKIDRSFVSRMSVDDTSRAIVRSVIKLGRVLSIDIVAEGIENESEA
                     AMMTNFGCTEMQGFYFSKALPADQMSKFLDTFQAKAPRSLHIVTTHGAAG"
     gene            complement(1187..1954)
                     /locus_tag="VI359_09210"
     CDS             complement(1187..1954)
                     /locus_tag="VI359_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974470.1"
                     /translation="MVQVPSKRANLIVRSRADAPHLKKAALAIEQAAWSHLGYLSYSR
                     AHYDYYTELLDAYPEYQLCLVDNETDYPVAVANSVPFVCSGPDDLPPEGWDWVVETAA
                     RNKDMRPNMLGGLAVSVPAVHRSKGYARVMIRSLIDLAKSKGLNGVVVPVRPSGKARH
                     PWVPIADYMAWTDDNGRPYDPWLRSHVSVGGKVVGPCARSMVVHEPISFWENWSKEPF
                     ERSGGYALEGALAPVEIDLDSQSGTYEEPNVWVWYDA"
     gene            2169..2573
                     /locus_tag="VI359_09215"
     CDS             2169..2573
                     /locus_tag="VI359_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974471.1"
                     /translation="MSVMYLERLNRALPSECGLHLDFSSGDSEESVELSREAADQMLQ
                     ALQVMTSEAAGEAQLTLTVLGHQVVRTPEAYGLLLRTQEFGDVVFSLPQVVLQKLITD
                     LTHLASSPPMSKTFDSVATTGNARGKIGTSHP"
BASE COUNT          552 a          791 c          712 g          592 t
ORIGIN      
        1 cccttctcca ttcatggcca gaatatatgc gtgaccgctt cggtgggcct cgccgtggcc
       61 cctgataacg gcgtcacggc cgacgaattg atgcgccacg ccgacatcgc gctctatgag
      121 gccaagaacc aaggccgtga ccgctccgtg ctcttctccg ccgaaatggc cgagcgcgtc
      181 gagcatcgcc gctcgatcga aatggagttg cgcaccgctc tcgaggccaa tcagttgcgc
      241 ctccattacc agccgatcat tgcatgtggc acgggcgcta ttgtcggagt ggaggcctta
      301 ctgcgttggt ttcatcctgt gcatggcgag atatcgcccg tggagttcat ccccatcgcc
      361 gaaaactccg gtcttcttcc ctcgctcgga gagtgggtcc tcgatcaagc aatgatggac
      421 tccaagcgct ggcccgatct ggaagttgcg gtgaacctct cgccggtgca gttccgccac
      481 gtcgacctca ctagcgccct gcgcacactc attacaaaac acaatgtgag tcctaaccac
      541 ttcgtgcttg agatcaccga gggtgtgctg ttggaagcaa gcgatcacac caactccatt
      601 cttgaggcca tacgcgccat gggcttcaag accgccctgg atgatttcgg cacgcgctat
      661 tcaagcttgg catacctttg caatttcaac ttcgacaaaa tcaagattga ccgctcattc
      721 gtcagcagaa tgtctgtgga tgatacctcg agagccatcg tccgatccgt tatcaagctc
      781 ggccgtgtac tcagtatcga tatagttgcc gaaggcatcg agaatgaatc ggaagccgcg
      841 atgatgacga atttcggctg caccgagatg cagggatttt acttttcgaa ggcgcttcct
      901 gcagaccaga tgtctaaatt cctcgacacc ttccaggcca aagctccacg gtctctgcat
      961 atcgttacga cacacggcgc cgctggctga ttcctgccca atgacccaga cagtcatagc
     1021 tttgcgctgg atcgatcctc gtaggctacg accttcgcgg aaggccgtgt ctcatttgcg
     1081 ccgtgcagtt tagcggacat cgtcatcgac gctgagcatg tggctcttga tgtcagcaga
     1141 cattccaagg ggccgtccga tgtctgctaa tgacccgaag cagaatctac gcgtcgtacc
     1201 aaacccaaac gtttggttcc tcataggtgc cgctctggct gtcgaggtcg atttcaactg
     1261 gcgccagcgc accttcgagc gcgtaaccac ccgatctctc aaacggctct ttcgaccagt
     1321 tctcccagaa cgagatgggt tcatgcacga ccatcgagcg tgcgcacggc ccaactactt
     1381 tgccacccac agagacatgg ctccgcagcc aaggatcata aggtcgcccg ttgtcgtcgg
     1441 tccatgccat gtaatctgcg atcgggaccc aaggatgccg agctttccct gaggggcgca
     1501 cgggcactac aacgccattt aatcctttgc tcttcgctag gtcgatgagc gagcggatca
     1561 ttacccgcgc atagcccttc gagcgatgga cggcgggcac cgagacggcc agccccccga
     1621 gcatattggg tcgcatgtcc ttgttgcgag cggccgtctc gaccacccag tcccatcctt
     1681 ctggcggcaa gtcatccggc cctgaacaga cgaacgggac gctatttgcg acggcaactg
     1741 gataatcggt ctcgttgtcc acgaggcaga gctgatattc cggataggca tcgaggagct
     1801 cagtgtagta gtcgtaatgc gcccgactgt agctgagata tccgagatgg ctccacgcgg
     1861 cctgttcgat ggcgagggca gccttcttca aatggggcgc atcggcacgc gagcgcacga
     1921 ttagatttgc ccgcttgctg ggaacttgga ccattggtga ttgcaaaggc caaccgcatc
     1981 tgggcagact gaaattctcc tgcccaccca tttttaacct taatagtcgc tgaaaacatt
     2041 gcaatgactg cacgttatta tcgttaacgc gcgatgcccc ccacgagaaa accccttggc
     2101 gtccttttct ttagaggtgg cgtcatactc tagacggcgg cagcggttat gttatctggc
     2161 gaactcatat gagcgtgatg tacctcgagc gtctaaaccg ggccttgccc tcagaatgcg
     2221 ggctgcatct cgatttcagc tctggcgact cggaggagtc ggtagagctc agccgggagg
     2281 ctgccgacca gatgctgcag gcgcttcaag ttatgaccag cgaagcagcc ggcgaagcgc
     2341 agctgacgct cactgtcctg ggtcaccaag ttgtgcgaac gcccgaggct tatgggctac
     2401 tccttcgcac gcaagagttt ggagacgtcg tattctcgtt gcctcaagtg gttctccaga
     2461 agcttataac cgatctaacg catctggcat cctcgcctcc gatgtcgaag acatttgatt
     2521 ccgtggccac gacaggaaat gcgcgtggca agatcgggac ttctcatcct taggcgatcg
     2581 acttcggtaa gacaactgcg cgctggatag tttgaggccc cccgccccgg gtttgacatc
     2641 tgctctt
//
LOCUS       DATDNW010000388         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_59044,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000388 DATDNW010000000
VERSION     DATDNW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59044"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VI359_09220"
     CDS             <1..322
                     /locus_tag="VI359_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974472.1"
                     /translation="EGLWQTGFFTHSELIEMATDTLRVMDRAVDVEGGEVPDTANKVM
                     LMPGFPCPLCRFPTYSWVEDMDNKVESYVLDFIRENHPGWDIEFGACDRCIEVYKLRA
                     DGVM"
     gene            339..938
                     /locus_tag="VI359_09225"
     CDS             339..938
                     /locus_tag="VI359_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY6974473.1"
                     /translation="MATDPKYGRRDFFKDSFLSVAKAAREFSIHSNEASEKPTPPLRT
                     DWLRPPGAVDEALFLERCTKCADCIKACPYGSIIFHQQDGTPVIFPDQVPCYLCDDTP
                     CIAACATEALLPVAGRDDIRMGLATVSHRLCTAAQGCHACASKCPTQALSMDFDAQLL
                     VVSAERCVGCGQCEQVCKTVNDHIAITVAPARLCPQKYP"
     gene            1192..2112
                     /locus_tag="VI359_09230"
     CDS             1192..2112
                     /locus_tag="VI359_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY6974474.1"
                     /translation="MTTPPAIKDSPAVERALSRSKLYLLLSWSLLYPEDEEFLDYMQC
                     GEFVEDGRAAIDALQAALDSFGGERAKQKIALLRKHFDHIEKLVASECVNWQLSDLQS
                     EHRRVFSNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFYKAFGVELSKDIHERLDHL
                     SVEFEFMHFLAYKESYSRCHDSAEKTQIVVDAQKKFVKNHIGRWVPLFCRMLVKKADS
                     GLFKIVADMMSDWMDFEAAFLNVTPQPYSEADYRPATFNAPEGQTYECGAQDQGNELS
                     LLLNETGAQNFMDHKDKEQDKEEGGPVGTA"
     gene            2208..>2843
                     /locus_tag="VI359_09235"
     CDS             2208..>2843
                     /locus_tag="VI359_09235"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEY6974475.1"
                     /translation="MRVVQTHNKHVVFGILLSALIVGVMLTIGQVPLAVSQPVTIPAK
                     TVKGPIPMDGANPIWESVPGVIVPLSGQLITTPMHPNISVKSVFVKAMTNGKEVGLRL
                     EWNDQTKNDTTIGPQDFRDQVAIMFPVNTSGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGIWSGNI"
BASE COUNT          654 a          736 c          806 g          647 t
ORIGIN      
        1 cgagggactc tggcagaccg gcttctttac ccactcagag ttgatcgaaa tggccacgga
       61 tacgctgcgt gtgatggaca gagccgtgga tgtcgaagga ggcgaagttc ccgacacggc
      121 caataaagtc atgctcatgc cggggtttcc gtgcccgctt tgccggtttc ccacatattc
      181 ctgggtcgag gacatggaca ataaggtcga atcctatgtg ctggatttca tccgcgagaa
      241 tcatcccggt tgggacatcg agtttggcgc ctgcgatcgc tgcattgaag tgtacaaact
      301 ccgagcagac ggcgtgatgt agtcaggccg aatctcacgt ggccaccgat cccaaatatg
      361 gccggcgcga cttcttcaag gactcgttcc tgtctgttgc taaagccgct cgtgaatttt
      421 ccatccatag caacgaggcg tctgagaaac caaccccgcc gctacgcacc gattggctgc
      481 gtcctcctgg tgcggtcgat gaggcgttgt ttctcgagcg atgcacgaaa tgcgccgact
      541 gcatcaaggc ctgcccgtac ggttcgatca tctttcatca acaggatgga accccggtga
      601 tttttccgga ccaagttccg tgttatctct gtgacgacac gccgtgcatc gccgcttgcg
      661 ccaccgaagc attgctgccc gtcgcgggac gggatgacat tcgcatgggc ctcgcaacgg
      721 tatcgcatcg gttgtgcacg gcagcgcaag gatgtcatgc ctgtgcgtcg aagtgcccga
      781 cacaagccct ctcgatggat tttgacgcac aactgctggt cgtgtcagct gaacgctgtg
      841 tcggctgtgg tcaatgtgag caagtctgta agacggtcaa tgatcatatc gcgataacag
      901 tcgcaccggc tcgtctttgt ccacagaagt acccctgaaa aaccaatgaa ttccgaggtg
      961 cgctcattcc tctagagaaa gaaaaaatgg agcaaattgg aactttttcc gataggccgt
     1021 gaagatgaga tcgggcttga catgccctga tcctttacct atcatagcga acctcagcag
     1081 tggtttgact gctcgcgccg aaaaaaacgg cgtggtgcgc gtcagacagg agagctgatt
     1141 ctcatgacga gtaaacagcc agtgcctgct tcacaatccg cagccgctgt gatgaccacc
     1201 cccccagcaa tcaaagattc tccagcagtc gaacgagctc tcagtcgcag caagttatat
     1261 ctccttcttt cgtggagtct gctttacccc gaggacgaag aatttctcga ctatatgcag
     1321 tgcggagaat ttgtcgaaga tgggcgtgct gcaatcgatg cgttgcaggc ggcgctcgat
     1381 agttttggcg gcgaacgagc caaacagaag attgcgctct tgcggaagca ttttgaccac
     1441 attgaaaagc tcgtggcgtc ggaatgcgtg aattggcaac tttccgacct gcaatccgag
     1501 catcggcgtg tgttcagcaa tgtgatcacg ctggactgtc caccgtatga aactctgttc
     1561 gggaatgacc acgtattcgc gcaatcgcac gtcatgggcg acattgccgg cttctacaag
     1621 gcgttcggcg tcgaattgtc gaaagatatt cacgagcggt tggatcatct cagtgtcgaa
     1681 ttcgagttca tgcacttcct cgcctataag gagtcttact cgcgttgcca tgacagcgcg
     1741 gagaagacac aaattgtggt cgatgcccag aagaaattcg tcaagaatca catcggacgc
     1801 tgggtaccgc tcttctgtcg gatgctcgtc aaaaaggctg attcgggcct gttcaagatt
     1861 gttgcggaca tgatgtcaga ctggatggac ttcgaagcgg cgtttttgaa cgtgacccca
     1921 cagccctact cagaggccga ctatcggcct gctaccttca atgccccaga aggccaaacg
     1981 tacgagtgtg gagcgcagga tcaaggaaac gaactgagcc tgttgctgaa cgaaacgggc
     2041 gcacagaatt tcatggacca taaggacaaa gagcaagaca aggaggaggg ggggccggtc
     2101 ggaaccgcct agcgagttga gtctttatcg tgtcgcggat ttttttgttt gcaggcagtc
     2161 atacttaggt tgctgtttaa tcatttaata gaggagggta tagtcacatg agagtagtgc
     2221 agacacacaa caagcatgtg gtgtttggca ttcttctctc tgcgttgatc gtcggcgtga
     2281 tgttgacgat cgggcaggta ccgttggccg tcagccagcc ggtgaccatt ccggcgaaga
     2341 ccgtgaaggg gccgatcccg atggatggcg caaacccgat ttgggaaagc gtcccgggtg
     2401 tgatcgtgcc gctcagcggc cagctgatta ccacgccaat gcatccgaac atctccgtga
     2461 aatcggtgtt tgtgaaggca atgaccaacg gtaaagaggt cgggttgcgt ctagagtgga
     2521 acgaccagac caagaatgat acaaccattg gtccgcagga cttccgtgac caggttgcca
     2581 tcatgttccc ggttaatacg tctggtgcgc caccgttcca gtgtatgggc cagtctggcg
     2641 ggaccaccaa catttggcgg tggaacgcgg agtggcagaa ggatctcggt aaggacagtg
     2701 ccggaatttg ggacgttgac gatcaatatc ccgggatttt ctgggactat tactttgaag
     2761 agcctgcagg cggcgtgacc tatccagacc gaattggccg cagcctcgga cctttcaatc
     2821 ccggaatctg gtccggcaat atc
//
LOCUS       DATDNW010000389         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_940806, whole genome shotgun sequence.
ACCESSION   DATDNW010000389 DATDNW010000000
VERSION     DATDNW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940806"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VI359_09240"
     CDS             complement(<1..182)
                     /locus_tag="VI359_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974476.1"
                     /translation="MRTMAGSAGRKTVSVSRGLMMLCNTCYDQVVADHLPDARNFVSN
                     HELLFDTICLGCIDINR"
     gene            complement(179..373)
                     /locus_tag="VI359_09245"
     CDS             complement(179..373)
                     /locus_tag="VI359_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974477.1"
                     /translation="MVLIYESDPLDDEHARGNFYHVCRGRIDRTPLTVPSVEQPYECP
                     QCGLDLEADDFWMAMQKGSV"
     gene            complement(450..2174)
                     /locus_tag="VI359_09250"
     CDS             complement(450..2174)
                     /locus_tag="VI359_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974478.1"
                     /translation="MKSLLRVLHYLRPYRRLALLTLLAAACATAMELLPPWLIKVVID
                     EVIQGKQMALLPWILGGLVASYGLKTTFASLRIRLNNRLEQTVVHDLRSHVFSALQRL
                     SINYFENRSTGEIMSRVTNDTEHVERIFIDGLEGALTASLTLIGITSMLFFLNWKLAL
                     LSLVPIPLLMVSASWFTRTVHGYYHHIRRVSGELSSYLQDALSGIRETMGFSKQNYEQ
                     QRFDRLSRAYSDSNLKAMYLWSLYSPGMMFLGSVGTVLILWYGAGEVQNGRLTLGELV
                     LFLSYLALFYVPINQIHSVNHMLQHALAASERVFEVLDTIPEVRDRPGVTAPVTRIRG
                     EVRFEQVTFHYRPDVPVLKGLTVSVRPGERVALVGPSGAGKSTLVKLLMRFYDVTGGA
                     ILIDGTDLRDLPLRYVRDHIGFVQQEPFLFNGSVRENILYGDLAATEQQLELAARAAR
                     AHEFIAELPEGYDTWIGERGIKLSVGQKQRVSIARVLLKDPPIVIFDEATSNIDTETE
                     VKIREALNELTKGRTTIIIAHRLSTLHDADRILVMDGGRLVEQGRHDDLLGRGGLYAA
                     LYEAQFQV"
     gene            complement(2202..2732)
                     /gene="def"
                     /locus_tag="VI359_09255"
     CDS             complement(2202..2732)
                     /gene="def"
                     /locus_tag="VI359_09255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY6974479.1"
                     /translation="MALLKIAKLGNPVLRRLADPVDPREIKSKEFQQLIDDMFETMLD
                     EPGIGLAAPQISHSIQLTVMGCEGEGGFPSTVLINPKIVYYGPHQVENWEGCLSVDGL
                     RGKVTRPSVVRVQALDRQGLSLDFEASGLYAVCIQHELDHLIGKVFLDRMTDLSTLTQ
                     LNEFTHYWQKEPASVI"
     gene            complement(2828..>3127)
                     /locus_tag="VI359_09260"
     CDS             complement(2828..>3127)
                     /locus_tag="VI359_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6974480.1"
                     /translation="MAKDQNSSIRKTAAFESRYVREKAVIPILIGMIADDERFVGLTA
                     VQSLWILTRHETEFHDWDISTKQDRQDWSVEWVEWWNSTKDSFELPEPRRPARTR"
BASE COUNT          721 a          924 c          797 g          685 t
ORIGIN      
        1 cgattaatgt cgatgcatcc gagacagatg gtatcgaaca gcagctcgtg gttcgacaca
       61 aaattcctgg catcgggcag gtggtcggcc acgacctggt catagcaagt attgcagagc
      121 atcatcagcc cccgcgacac cgacaccgtt ttgcgtccag cacttccagc cattgtcctc
      181 acaccgagcc cttctgcatc gccatccaaa aatcgtccgc ttcgaggtcg agcccgcact
      241 ggggacattc gtagggctgc tcaacggacg gaaccgttaa cggggttcga tcgatccgcc
      301 cacggcagac atgatagaag ttccccctgg catgttcgtc atccagcgga tcgctctcgt
      361 aaatcagcac catacgcgac tcactcttct caagcgaatt gcagtatacc gcggctgtcc
      421 gggaacccgc aacgaatggc acctcgctgc tacacctgaa actgcgcctc atagagggcc
      481 gcatacaggc cgccccgacc gagcaagtca tcgtgccgcc cctgttccac caatcgcccc
      541 ccatccatga ctaaaatacg atcggcgtcg tgcaacgttg acagtcgatg cgcgatgatg
      601 atcgtcgttc ggcctttagt taattcattc aatgcttctc tgatcttcac ctcggtttcg
      661 gtatcaatgt tcgacgtcgc ttcatcaaag atcacgatcg gaggatcctt caacaacacg
      721 cgcgcgatcg agacccgctg cttctgaccg acagacagct tgattccccg ctcaccgatc
      781 caggtatcgt aaccttccgg aagttccgca atgaactcgt gggctctggc ggcgcgagcc
      841 gcgagttcaa gttgctgctc ggtcgccgca agatccccgt agagaatgtt ttcgcgaacc
      901 gatccgttga aaaggaaggg ttcctgttga acaaatccaa tgtgatctct cacatagcgg
      961 agcggcagat ctcgcaggtc ggtcccatca atcaagatcg ccccgcccgt cacatcgtag
     1021 aaccgcatga gcagcttcac taacgtgctt ttccccgccc cgcttggccc gacgagcgcc
     1081 acccgttctc caggacgtac cgacaccgtc aatcctttca ggaccggcac gtcgggacga
     1141 tagtgaaacg tcacctgctc aaacctgacc tcaccgcgta tccgcgtgac cggtgccgtg
     1201 acgcccggac gatcgcgcac ttcgggaatc gtatcgagca cctcaaagac cctttcgctg
     1261 gccgcgagcg cgtgttgtaa catgtgattc acggagtgaa tctgattgat cggtacgtaa
     1321 aataaggcga gataggagag aaagaggacc agctccccca gcgtcaagcg accattctgt
     1381 acttccccgg caccatacca gaggattaag accgtgccca cactgccgag gaacatcatc
     1441 cccggcgagt agagcgacca gaggtacatc gccttgagat tactgtcgct gtaggcccgg
     1501 ctcaagcgat cgaatcgttg ctgctcgtag ttctgtttgg agaaccccat cgtctccctg
     1561 atccctgaga gagcgtcttg gaggtagctg ctgagttccc ctgaaaccct gcgaatatgg
     1621 tgatagtagc catgtaccgt ccttgtaaac caactggccg acaccatcag aagaggaatt
     1681 ggtactagcg acaggagcgc gagcttccaa ttgaggaaaa agagcatact cgtaatgccg
     1741 atcagcgtca gcgaggccgt taatgccccc tcaagcccgt cgatgaagat ccgttcgaca
     1801 tgctcggtat cgttcgtcac acgcgacata atctcgccgg tcgagcggtt ttcgaaatag
     1861 ttgatcgaca gccgttggag cgcgctgaag acgtgactcc gcaaatcgtg gacgaccgtc
     1921 tgttccaacc ggttgttgag tctgatacgg agtgatgcaa aggtggtttt taagccatag
     1981 gatgccacca gaccgcccaa gatccacggt aaaagcgcca tctgtttacc ttggatcacc
     2041 tcgtcgatca cgaccttgat cagccagggg ggaagcaatt ccatggccgt tgcacaggct
     2101 gcggccagca aagtcagcag cgccagtcgg cggtacgggc gaagatagtg gaggacgcga
     2161 aggagggatt tcacaagttg atcagtcggt tgtccatcag ctcagatgac cgaggctggc
     2221 tccttctgcc aataatgcgt gaattcgttg agttgtgtga gggttgaaag atccgtcatg
     2281 cggtctaaaa atactttccc gatcaaatga tccagttcat gctggatgca gaccgcgtat
     2341 aaaccgctcg cttcaaaatc aagcgatagg ccttggcgat caagcgcctg aactcggacc
     2401 actgacggtc tcgtcacctt gccccgtaag ccatcgacac tgaggcagcc ttcccagttc
     2461 tccacttggt gcggcccgta gtagacaatc ttggggttga tcaagacggt cgacggaaac
     2521 ccgccctcac cttcgcaccc catcaccgtc agctgaattg aatgagagat ttgtggcgcc
     2581 gctaagccga tccccggttc atcgagcatc gtctcgaaca tgtcatcaat gagttgctga
     2641 aattctttac tcttaatctc ccgaggatcg accggatctg ccaatctccg gaggacagga
     2701 ttcccgagtt ttgcaatttt tagcagcgcc atactctacc taacacaata cctgcttatg
     2761 aaggtgtgtc atctgttgat gctatagcat acaggttttc gagcctgcaa ccgcaggaat
     2821 gcagggatta gcgcgtgcgg gccggtcgtc gaggctccgg caactcgaat gaatcttttg
     2881 tgctgttcca ccactccacc cactcaacgg accagtcctg ccgatcctgc ttggtagata
     2941 tatcccaatc gtggaattct gtctcgtgcc tggtcaaaat ccacaaggat tgaaccgccg
     3001 tcagtcccac aaaccgttcg tcatccgcaa tcatcccgat caggataggg atcacggctt
     3061 tttcgcggac atagcgcgat tcgaatgccg cagtcttccg gatcgaagaa ttttgatcct
     3121 tggccat
//
LOCUS       DATDNW010000390         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_132791, whole genome shotgun sequence.
ACCESSION   DATDNW010000390 DATDNW010000000
VERSION     DATDNW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132791"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="plsY"
                     /locus_tag="VI359_09265"
     CDS             complement(<1..578)
                     /gene="plsY"
                     /locus_tag="VI359_09265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY6974481.1"
                     /translation="MDNQFLWTLMAIAGYVLGAIPFGLVVSKAMGLPDPRTVGSKNVG
                     FTNVLRVSGTKAGVLTLCGDMGKGWVMGWAAMQWLTDEPFIMVVALSPILGHVFSPFL
                     SFKGGKGVATALGSVLGLSPSMGVLLLLIWVGTVAIWRYSSGGALAAFGLFPVVAIVN
                     EQRQEFLVFSVVVSALIWFKHKDNIVRLWKGT"
     gene            complement(623..1219)
                     /gene="pgsA"
                     /locus_tag="VI359_09270"
     CDS             complement(623..1219)
                     /gene="pgsA"
                     /locus_tag="VI359_09270"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY6974482.1"
                     /translation="MNWVIDMWKQVGQESINVPNLLTLTRILLIPVFVVLFATPDPHR
                     SIIAAIVFAVAAVTDLLDGYLARRSGQVTKLGKLLDPIADKLLVLSALILLVNVDRVS
                     ALVAILIIAREVAVTGIRAIAATEGMIIEAETTGKYKMALQVIAIVLLILEGTALSAL
                     GNLHLAGIVTLYLSLILGYVSGAQYVWSFWKQVATKGL"
     gene            complement(1241..2269)
                     /locus_tag="VI359_09275"
     CDS             complement(1241..2269)
                     /locus_tag="VI359_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY6974483.1"
                     /translation="MRRSKTEKLKIKETAKASGEQTGSSLNEGRRVLQVEAHAVQTLI
                     DRLDERFTEAVDLLYNCKGKVVVSGMGKSGLIGQKIAATLASTGTPAFFLHPAEGLHG
                     DLGMLARRDALIAISNSGETKEILQLLPYIERMGVPTVGLTGKMGSTLAKHSDVVLDV
                     SVSEEACPMGLAPTASTTATLAMGDALAVALLQKRGFKSEDFAQFHPGGTLGRRLLIK
                     VRDLMHVGDEVPRVQEQATGSTAISEISAKKLGMTTVTDRTGVLLGVITDGDLRRFVQ
                     QGGDIIKATAGTLASRTPRTIEPDDLAARAVEMMERFSITTLVVTEGERRIVGVIHLH
                     DLLKNGIV"
     gene            complement(2242..>2440)
                     /locus_tag="VI359_09280"
     CDS             complement(2242..>2440)
                     /locus_tag="VI359_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY6974484.1"
                     /translation="SSGQREFVEPLACAAAGAGVDGFFMEVHPNPDDALSDGPNMVPL
                     HQLKAILERVMRICDAAKPKN"
BASE COUNT          533 a          768 c          671 g          468 t
ORIGIN      
        1 tccgttccct tccagagccg gacaatatta tctttgtgct taaaccagat cagcgcgctg
       61 accaccaccg agaaaacgag aaattcctgt cgctgttcgt tcacgatcgc caccaccgga
      121 aatagcccga atgccgcgag agcgccgcct gaggaatagc gccagattgc aacggtcccg
      181 acccagatca gcaacaggag cacccccatt gagggcgaca ggccgagcac ggatcccaag
      241 gctgtcgcca cccctttgcc tcctttgaag ctcagaaacg gcgaaaagac atgaccgaga
      301 atcggcgaga gggcgacgac catgatgaac ggctcatcgg tgagccactg catggcagcc
      361 cagcccatga cccacccctt gcccatgtcg ccacacaggg tcaggacacc agctttcgtg
      421 cccgatacgc gaagcacgtt ggtgaagccg acgttcttgc tgccgaccgt gcgcggatcg
      481 ggcaggccca tggccttcga cacaacaagg ccaaacggaa tggcaccgag cacgtagccg
      541 gctattgcca tgagagtcca gaggaactga ttgtccatgg attaggggat gtcggagaaa
      601 cgacgtatgc actcaagcac ggctagagtc ccttagtcgc tacttgtttc cagaagctcc
      661 acacatattg agcgccggac acgtacccga ggatgagcga caggtagagg gtgacaatcc
      721 cggccaagtg gagattaccc agagccgaca gggccgtgcc ttccagaatc agtaagacga
      781 tcgcgatcac ttgcaacgcc atcttgtact tgccggtcgt ttcagcctcg ataatcatcc
      841 cttcggtcgc cgcaatggca cggatgcctg tcaccgcgac ctcgcgggca atgatcagaa
      901 ttgccacaag cgcgctgacg cgatccacgt tgaccagcag aatcaacgcc gacaagacga
      961 gtagcttatc ggcaatcgga tcgagcaact tgcccagctt ggtgacttgc cctgaacggc
     1021 gcgccagata gccgtcgagc agatcggtca ccgccgccac ggcgaaaacg atggccgcga
     1081 tgatggatcg atgcggatcc ggcgtcgcaa acagcaccac aaagaccgga atcaggagaa
     1141 tcctcgtgag cgtgagcaga ttcggaacgt tgatcgattc ctgccccacc tgcttccaca
     1201 tgtcgatgac ccagttcacc gtgtctctca gctcctgtgg ctatacaatg ccgttcttca
     1261 agagatcatg cagatgaatc acgccgacga tccgccgttc cccttcagtc accacgagcg
     1321 tcgtgatcga gaagcgctcc atcatttcga cggctcgtgc cgccaagtca tccggttcga
     1381 tcgttcgcgg cgtgcgggaa gccagtgttc cggcggttgc cttgatgata tcgccgccct
     1441 gctggacgaa ccggcgcaaa tctccatccg tgatcacacc gagaaggacg cccgtccgat
     1501 cagtcaccgt cgtcatgccc agtttcttgg ccgagatctc cgaaattgca gtcgaaccgg
     1561 tcgcctgttc ttgcactcgg gggacttcat cgccgacatg catgagatca cggaccttga
     1621 tgagcaagcg gcggccgagt gtgccgcccg gatggaactg cgcaaagtct tcggacttga
     1681 acccccgctt ctgaagtagc gccacggcca acgcatcccc catggccaag gtcgccgtcg
     1741 tgctggccgt cggggccaat cccatcgggc aggcttcctc cgacacggag acgtccaaca
     1801 ccacatcgct gtgcttcgcc aaggtcgaac ccatctttcc cgttaagcca accgtcggaa
     1861 ctcccatccg ctcgatgtac ggcaacaact ggaggatttc cttcgtctcg ccgctgttgg
     1921 aaatcgcaat gagggcatcg cggcgggcga gcatccccag atcgccatgc aacccttcag
     1981 ccgggtgaag aaagaaggct ggtgtcccgg tgctggccaa cgtcgcggca atcttctgcc
     2041 caatgagacc ggacttaccc atgccggaga cgacgacttt ccctttacag ttatagagca
     2101 gatccacagc ttccgtgaat cgctcgtcga ggcggtcaat aagggtttgg acagcatggg
     2161 cctcgacctg gagcacccgc cggccttcgt tgagacttga gccggtctgt tcgccagacg
     2221 cttttgcagt ctctttgatc ttcagttttt cggttttgct gcgtcgcata tccgcatcac
     2281 ccgttcgaga atcgccttca actgatgcag agggaccatg ttcgggccat cggagagggc
     2341 gtcatcgggg ttcggatgca cctccatgaa gaagccatca acgcctgctc ccgctgccgc
     2401 acaggcgagc ggttcgacga attctcgttg gccgctggac
//
LOCUS       DATDNW010000391         3352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_605679, whole genome shotgun sequence.
ACCESSION   DATDNW010000391 DATDNW010000000
VERSION     DATDNW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605679"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="ftsH"
                     /locus_tag="VI359_09285"
     CDS             complement(<1..1500)
                     /gene="ftsH"
                     /locus_tag="VI359_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY6974485.1"
                     /translation="MNKKISFSIWYVFLAIWAVILVHDFIHALQKIEELPYSEFKTLV
                     ASNKVAEVSVTAQKVTGKLKPEGEAKEEKLFSSVRVEDPDLTKELTAHGVKVTGVIES
                     TFFRDVLSWLLPVVVFIGIWYFIFQRLGQAQGGFMRVGQSKAKIYMEKDIKVTFADVA
                     GVDEAKEELREVIEFLKTPEKFTKLGGKIPKGILLVGPPGTGKTLLARAVAGEAAVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQAKGKAPCIIFIDELDALGKARGMGPMAHEERE
                     QTLNQLLVEMDGFDPRIGVILMAATNRPEILDPALLRAGRFDRHVLVDRPDKVGRLAI
                     LRVHAKQVTLAPDADLETVAAMTPGFSGADLANVINEAALLAVRRGKEEVGLSELQEA
                     VERVIGGLEKKNRVLNKMEKERVAHHETGHAIVALSIPGADTVQKISIIPRGIAALGY
                     TLQLPTEDRFLMTQSELENKIAVLLGGRIAEELIFGEASTGAQNDLVKAT"
     gene            complement(1513..2670)
                     /locus_tag="VI359_09290"
     CDS             complement(1513..2670)
                     /locus_tag="VI359_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974486.1"
                     /translation="MKPADRIAVWCRRIAVMTRKELMQLFRDIPLIAFLIYSFTFAIF
                     VTGNGIKTQLRNAGLLVYDADRSVSSRELVSRFHPPYFRFDGELAHPDEEFQRLDRGS
                     SMMVLDIPTRFHEALAAGEPTAVQLLVDTTNSPQGLSAAGYAARIVGQFGQEVALARN
                     GLADSSLTSVPMILSDHRVWFNQDQNETWFESISHLLRMITIFSVLLPAAALVREKER
                     GTVEQLLVSPLTPLQIILPKVVAMTLVILIATAVGLFGVMRLAFGVPIRGSVGLLFSL
                     TALFIVATAGMGVFAATITRNQAQVGMLTLLVVAPMLLLSGIFTPLEMMPGWVRHLMA
                     FSPLRYFIEIVNGILLKGIGIEMLWSQALSMGVVGGSLFGFGLWRFRRQFE"
     gene            complement(2667..>3352)
                     /locus_tag="VI359_09295"
     CDS             complement(2667..>3352)
                     /locus_tag="VI359_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974487.1"
                     /translation="GLSRDEAQRRLRPLTLEVRYLYNEEVHSVWSTVPALIMVSLMVS
                     SPLLTALGIVREKETGSIYNIYSATVSRLEFLTGKLFPYVMISAVNAMVLWLMAVFLF
                     AVPFKGNLLLFVATSLLFVLTSTGIGLVVSLLVRTQQAALIITVLLSTVPTILFSGLV
                     VPVSSLSQATQFQAHLFPGMYYTNIVRGTFLKNIGPEILWTDILALGAYATVLGTIGY
                     RLFTKRPRA"
BASE COUNT          794 a         1038 c          865 g          655 t
ORIGIN      
        1 ggtcgctttg acgagatcgt tctgcgctcc cgtcgaggcc tcgccgaaga tcaactcctc
       61 tgcaatccgg ccaccaagca ggaccgcaat cttgttctcc agctcagatt gcgtcatgag
      121 aaaccgatcc tccgtcggca actgaagggt atatccgagc gcggcaatgc ctcgtggaat
      181 aatggagatt ttctgtaccg tgtccgctcc gggaatcgac aacgccacga tcgcatgacc
      241 ggtctcatga tgggctaccc gttccttctc cattttattc aggacacgat tctttttctc
      301 caggccgcca atcactcgct cgaccgcttc ttgtaattcg gacagcccta cttcttcctt
      361 tccccgacgg accgccaata gtgcggcttc gttgatgacg ttcgcgagat cagcaccgga
      421 aaagccgggc gtcatcgctg cgacagtctc aagatccgcg tccggcgcga gagtgacttg
      481 cttggcatga acacggagga tggcgaggcg accgaccttg tcggggcgat caacaagcac
      541 atgccgatcg aaccggcccg cccgcaacag cgcaggatca agaatctccg gacggttggt
      601 tgccgccatg agaatgacgc cgatgcgggg atcgaagccg tccatctcaa ccagcaactg
      661 attcagcgtt tgctcccgct cctcatgcgc catgggtccc atcccgcggg ctttgccgag
      721 cgcatccaat tcatcgatga agataataca gggggctttg cccttcgcct gttcaaacag
      781 atcccgcaca cgggccgcgc cgactccaac gaacatttcc acgaactccg atccgctgat
      841 gctgaaaaac ggcacggcgg cttcgcccgc cacggcacgg gccaacagag tcttgccggt
      901 tcctggagga ccgaccaaca agattccctt gggaatcttt ccaccaagtt tcgtaaactt
      961 ctcgggcgtc ttcagaaact cgatgacttc gcggagttcc tccttcgctt catctacgcc
     1021 ggccacgtcg gcgaaggtca ccttgatgtc cttctccata tagatcttgg ccttggactg
     1081 tcccacgcgc atgaagccac cctgggcctg tcccaaccgt tgaaagatga agtaccagat
     1141 cccgatgaac acgacgaccg gcaacagcca ggacagcaca tcacggaaaa atgtactctc
     1201 aatcacgccg gtaactttga caccatgggc cgtcaattcc ttcgttagat ccggatcttc
     1261 aacacggaca gaactaaata atttctcctc cttcgcctcc ccttccggct tcagcttgcc
     1321 ggtcactttc tgagcggtta ccgacacttc cgccaccttg ttggaagcga ccagcgtctt
     1381 aaactcgctg tacggcagct cttcgatctt ctggagtgca tgaataaaat cgtggacgag
     1441 aatgaccgcc caaatcgcga ggaaaacgta ccagatggaa aacgaaatct tcttgttcat
     1501 ggatacactc cgttactcaa actgacgccg gaagcgccac aggccgaagc cgaacaagga
     1561 accgcccacc acccccatcg acagggcctg actccacaac atctcaattc caatcccttt
     1621 caacagaatg ccgttcacga tttcgatgaa ataccggagc ggggagaatg ccatcagatg
     1681 gcgtacccag ccgggcatca tctccagcgg cgtgaagatg ccggagagca acagcatggg
     1741 agcgaccacg agcaacgtca acatccccac ctgtgcctga ttcctcgtga tggtcgcggc
     1801 gaacaccccc atccctgccg tcgccacgat gaagagagcc gtcaaggaaa acagcaggcc
     1861 gacgctccct ctgatcggca caccgaaggc caatcgcatg acgccaaaca aacccaccgc
     1921 tgtcgcgatc agaattacca acgtcattgc cacgactttg ggaagaatga tttgcagcgg
     1981 ggtcaacggc gatactagaa gttgctccac cgtgccgcgc tctttctccc gcaccaatgc
     2041 cgccgccggc aacaacacgg aaaaaatcgt gatcatccgc aagaggtgcg aaatggattc
     2101 gaaccacgtt tcattctggt cttggttgaa ccagacgcga tggtcgctta ggatcatcgg
     2161 cacgctcgtc aaagacgagt ccgccaaccc atttcgcgcc aatgccactt cctggccgaa
     2221 ttgacccacg atgcgggcgg cgtagccagc cgccgacagc ccttgcggcg agttcgtcgt
     2281 atcgacaagc aattgcacgg cggtcggttc ccctgccgcc agtgcctcat ggaagcgggt
     2341 aggaatgtcc aacaccatca tcgaacttcc ccggtccagc cgttgaaatt cttcgtccgg
     2401 atgcgccaac tcgccatcga atcgaaagta ggggggatgg aatcgagaga ccagctcacg
     2461 ggatgacaca cttcgatcgg catcgtagac caacagcccg gcatttctca gttgcgtctt
     2521 gatcccgttt ccggtgacga agatcgcgaa agtgaatgaa tagatgagaa atgcgatgag
     2581 cgggatgtca cggaacaact gcatcaactc tttccgcgtc atcacggcaa tccgtcggca
     2641 ccagacggca atgcgatcgg ccggtttcat gcgcgtggcc tcttggtgaa caatcgatag
     2701 ccgatagttc ccagcaccgt tgcataggcc ccgagggcaa gaatgtccgt ccataagatt
     2761 tccggaccga tattcttcag gaatgtgccc ctgacgatat ttgtgtagta catccccgga
     2821 aacaggtgcg cttggaactg cgtcgcctgg ctcagggacg agaccggcac gaccagcccg
     2881 gagaagagaa tcgtgggtac cgtgctcagt aacacagtga tgatcagggc agcctgctgc
     2941 gtcctgacca gcaatgagac caccagaccg ataccggtac tagtcaatac gaacagaagg
     3001 gacgtcgcga caaacaacag caggttcccc ttaaacggca cggcaaacag gaacacggcc
     3061 atcagccaca acaccatcgc gttgactgcg gagatcatga cgtatggaaa cagcttaccg
     3121 gtcaggaact ccagccggct cactgtggcg ctgtagatgt tgtagatcga accggtttcc
     3181 ttctcccgga ctatgccgag cgcggtcaac aggggggacg agaccatgag cgaaaccata
     3241 atgagcgcgg gcacggtcga ccagacgcta tgcacctcct cattatataa ataccgcact
     3301 tcgagggtga gagggcggag ccgccgttgc gcctcgtcgc gtgacagccc tc
//
LOCUS       DATDNW010000392         9308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_533965, whole genome shotgun sequence.
ACCESSION   DATDNW010000392 DATDNW010000000
VERSION     DATDNW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533965"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1487)
                     /gene="guaB"
                     /locus_tag="VI359_09300"
     CDS             complement(21..1487)
                     /gene="guaB"
                     /locus_tag="VI359_09300"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY6974488.1"
                     /translation="MLEQEPRTGLTFDDVVLVPAKSQVLPSEVITHTRLSRRIQINIP
                     IVSSAMDTVTESRLAIAMAREGGMGIIHRVLSPADQAAEVDKVKKSESGMILDPVTIS
                     PEQTIRDAHELMAKYRISGIPVTKNKKLVGILTNRDLRFESRMELKVSQVMKRDKLVT
                     APEGTSLEKAREILHEHRIEKLPVVNKHFELKGLITIKDIEKRIKYPTACKDGHGRLR
                     VGAAVGVGPETEDRVAQLKKAGVDLVVVDTAHGHAQAVLDTVKMIKKHYSDLELVAGN
                     IATAEAAKDLLKAGVDAVKVGVGPGSICTTRIVSGAGMPQLTAIADCAKALDGSGVPI
                     IADGGVKFSGDITKALAAGASSVMLGGLLAGTEESPGETVLYQARTYKVYRGMGSIGA
                     MERGGGDRYGQAGRQAQKLVPEGIEGRVPYKGPVSAVIYQLVGGVKSGMGYCGCKTIP
                     ELQQKATFIRQTVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            complement(1519..4488)
                     /locus_tag="VI359_09305"
     CDS             complement(1519..4488)
                     /locus_tag="VI359_09305"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY6974489.1"
                     /translation="MVKAWLFDEMFRLKRSVLRADAVQDEWGGGDSIAEAEELPPPPE
                     NVKAKPGNGRITLTWDAVPDAMYYNVYFQTTKGVQIKFSELTRPIAGEEDFKATIGVK
                     KEKATCIEGPSSPFIHDDLANGTCYHYVVTVVTAKGESPESQEAMAIPAPYLLAMCIG
                     REGVDDGEFSSPTGITLDKDGNIYVADTDNHSIQKFDKTGKFLARWGAEPSSQEGSFY
                     YPRGLAIGSDDVLYVADSGNNRVQRFDLDGNVMQAWGKFGMAWRGADMGRFDVPWGLT
                     TDQEGNLYVSDTSNARIQKFKADGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNIYVAD
                     EGNNRIQKFDARGSFLAKWGREGSGPGQFKSPWGVFCDALGNVYVVDTGNHRIQKFDG
                     NGTFLCSWGNRGRTEGQLNFPYGVAVDKEGCVYVVDSGNNRVLKYVPTEEELNRGKDD
                     APKSQAVSTTPPPHSVAVKAGDTETFLSWMEVPGAVSYNLYFGTSQGITKQTATRIEG
                     VTNPYTHTGLTNDTAYFYAVSAVFQDQTESALSSEVTATPVLIDITAPQNPYAVINHG
                     AFMTNSPEVVVTISAHDIDTGVGAYFISESPMTPMAGTPGWVDVTPALKFGATIPFIL
                     SPGDGQKVIYVWFKDISGNVSTPASSTILVNTSGYVCVAKWGRPGRGASLLHGGEFIA
                     PMYGLCVDQQGSLFVVDNGNNRVQKFDNAGNFIILWGNFGSANSNFHNPSGVACDGKG
                     DVWVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNSPWGIAIDRVRGYVYVVDSAN
                     FRVQKFDMTGEFIMSWGSFGSADGQFYFPRGIAVDQSDGSVYVVDMGNHRIQKFDTST
                     NVLPQLLTKWGGSSEPGHASSVLAQEAGQLRSPWGIAVDGAGDVYVTDTGNHRIEKFD
                     KEGNFITQWGGFGNGDGHFNFPYGIAVDAKGNVFVVDSGNTRVQQFMPAEEGGERLQE
                     EAEAAAEIEKAQGASTT"
     gene            complement(4706..5470)
                     /gene="scpB"
                     /locus_tag="VI359_09310"
     CDS             complement(4706..5470)
                     /gene="scpB"
                     /locus_tag="VI359_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY6974490.1"
                     /translation="MSLTVTQAMEDISCVGGEVAEGGISNSNGHAEAVDRRELKAILE
                     AVLFVSSDPVPVARLVSVLGAVKKTDVEQALAQLKQDLEEDGRGVQLVQVAGGYRLMT
                     KPDYAPWIKKLEKAKTAQKLSRSALESLAIIAYKQPLVRSEIEQIRGVETSGVLRTLL
                     ERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGLQDLSQLPPLREFKELGDAEQAFLPME
                     EETGHMDRSMLGEESESSDITLALASPLLTEPVEQA"
     gene            complement(5467..6246)
                     /locus_tag="VI359_09315"
     CDS             complement(5467..6246)
                     /locus_tag="VI359_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY6974491.1"
                     /translation="MEQTELPYQVRIDNFEGPLDLLLHLIKKSEINIYDIPIALIARQ
                     YLEYLEAMKDLNLTVAGEFLVMAATLLQIKSKMLLPVDETTEDEEDGPDPREELIRRL
                     LEYKQYKDAARQLDNQGRLWRELYSREPSPPVEAESEEALLEDINLFDLVDALRGILD
                     RNPGKQLLEIIPDNLTVRDRMSAILEALEGQESVTFSSLFESSSHRMVIIVTFLALLE
                     LIRLRTARVFQGENFGPILVSRTFSLVPDPAELDDEEWRGA"
     gene            complement(6344..7471)
                     /locus_tag="VI359_09320"
     CDS             complement(6344..7471)
                     /locus_tag="VI359_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY6974492.1"
                     /translation="MTSSADAVSALPSTGAFRIRAVMFIRQRIGLVAVVAAIWGSVLE
                     GGAASCEIISGATQSEAALMLSLSADCTDGEREKRAVEAIQVIDALKRGKDIDLSGVM
                     IHGDLLLDVLATGPMPRDLKEVAAIESRDIRVVQGAISIVNSIVYGAIRHQSAQGQLV
                     FRSPVTFTGTRFEQTVDLSRSVFMRPVLLSGAIFLRESYFVQARFLNDVVAEKTAFGP
                     HTRFHRARFHGAATFQQSGFSGLAEFLEVEFERDANFSRTYFKLGTGFSGSRFRGMAD
                     FSESLFDREAFFPFVVFEGDAYFRRATFRSTADFSNAEFRGRDDFAKVLFEGGPEFSG
                     AKRTGKEKDLLGLESPKVQYVITLSLLVFSALLIAYLIKSR"
     gene            7552..8196
                     /locus_tag="VI359_09325"
     CDS             7552..8196
                     /locus_tag="VI359_09325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY6974493.1"
                     /translation="MRTKLSIIRVILVSLACSIPTALFADNVIELPVLAAVGINNQGA
                     FEVLLIWWDKKPQPDPPQLQWIAGRVRLGSTPLGSMSQAFSYAIERTPSVQHSGTVSV
                     AGVAYAPTGTDGPSAGAAMAVGFIALFKGDKVQRGIALTGTFEPGGKIGFVGSIPDKL
                     RAAAREGYRTILVPRGHIYNERWNLNELALELNVSVKEVETIDEAYQLMTGQRI"
     gene            complement(8193..8654)
                     /locus_tag="VI359_09330"
     CDS             complement(8193..8654)
                     /locus_tag="VI359_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974494.1"
                     /translation="MSHFLFIAGALRERSSEDSAELQLSHGLWGLCTSLIKTNLETYL
                     EPQSRGLVYVLKAGLCAEFQIRSLVLPFRDLDELLKDDLKTEARFGFVRVNILRRWVS
                     SPQESQAILQSVLQIPDQAELTRRLALGMHRLTETEYEAIVRGIGEPAGTR"
     gene            complement(8700..9134)
                     /locus_tag="VI359_09335"
     CDS             complement(8700..9134)
                     /locus_tag="VI359_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974495.1"
                     /translation="MAKKKKRATKSHSAPASKKAAVKKKAVKKAAPKSKRKAVPKKKA
                     AAKKPTVQTKAAGAPAKKFVPSAEEETPRQKVIAKPAVPLEPKDEELDEEVGEEELEI
                     AGEGEMDDEMEEDLSLDDEDEDVDYIEKSEDLLDDSDDYRTH"
BASE COUNT         1939 a         2846 c         2406 g         2117 t
ORIGIN      
        1 tgagagctga tagctttcga tcactcccaa tccatccgat agttcggggc ctctttcgta
       61 atgatcacgt catggacatg actttcgcgg aggccagcga ccgtctggcg gatgaacgtg
      121 gccttttgct gcagttccgg aattgttttg cacccgcaat agcccatacc ggatttcaca
      181 ccgccgacaa gctggtaaat cacggccgac accggccctt tgtagggcac gcgtccctca
      241 atcccttccg gcaccagctt ctgcgcttga cgcccggcct gtccgtaacg atctccgccg
      301 ccgcgctcca tcgcaccgat cgaccccatg ccgcgataga ccttatacgt tcgggcttga
      361 tatagcacag tctcgcccgg tgactcttcc gtccccgcca gcaaaccgcc gagcatcacc
      421 gaggaggcgc ctgccgcgag ggccttggtg atgtcccccg aaaatttgac gccgccgtcg
      481 gcgatgatcg gcacaccgct tccgtccaaa gctttggcac aatccgcgat ggcggtcagc
      541 tgtggcatgc cggcccctga cacgatgcgg gtggtgcaga tcgagccggg cccgacaccg
      601 actttgaccg catcgacccc tgccttgagc aggtccttcg ccgcttcggc cgttgcaatg
      661 tttccggcaa ccaattccag atccgaataa tgcttcttga tcatcttaac cgtgtccagc
      721 accgcttggg catggccgtg ggcggtatcg acaaccacca ggtccacacc ggctttcttg
      781 agttgtgcca cgcgatcttc cgtttccggc ccgaccccga ccgccgcgcc gacgcgaagc
      841 cgtccatgtc catccttaca ggccgtggga tacttgatgc gcttttcgat atccttgatc
      901 gtgatcaagc ctttgagctc gaagtgtttg ttcacgactg gtagtttttc gatgcggtgt
      961 tcgtgcagaa tctcgcgagc cttttccaga ctggtgcctt ccggagcggt caccagcttg
     1021 tcccgcttca tcacctgaga cacctttaat tccatgcggc tttcgaaccg cagatcgcgg
     1081 ttcgtcagaa tgccgaccag tttcttgttc ttcgtcacgg gaatgccgga gatgcgatac
     1141 ttcgccatca actcgtgcgc atcccgaatc gtttgctccg gagaaatcgt gacggggtcc
     1201 agaatcatgc cgctctcgga ctttttcacc ttgtccactt ccgcagcttg atcagccggt
     1261 gacaagacgc gatgaataat ccccattcca ccttcgcggg ccatcgcgat cgccagccgt
     1321 gattccgtca cggtatccat ggcggaactg acgataggaa tgttgatctg gatccgacgg
     1381 ctgaggcgag tgtgcgtaat gacttcgctc gggagcacct gcgacttcgc aggcaccaga
     1441 acgacgtcat cgaaggtcaa tccagtacgc ggttcttgct ccaacatggt gccccttcat
     1501 tgcctccgga tcagtgactt atgtcgttga cgccccttgc gccttttcaa tttccgccgc
     1561 tgcctctgcc tcttcttgga gccgttcgcc gccttcttca gccggcataa actgctgcac
     1621 gcgggtgttg ccgctgtcga ccacaaacac attccccttg gcatcgacgg caatcccgta
     1681 ggggaaattg aagtgcccgt ctccgtttcc gaacccaccc cactgcgtga tgaagttccc
     1741 ttccttatcg aacttttcaa tccggtggtt tccggtatcg gtcacgtaca catctccggc
     1801 tccgtcgacc gcaatacccc atggagaccg aagctgtccc gcttcctgcg ccaggacgct
     1861 gctcgcgtgg ccaggctcgg aactgccgcc ccacttggtc agcagctgcg gcaagacgtt
     1921 cgtgctggta tcaaacttct ggatgcgatg atttcccata tccaccacat agaccgaccc
     1981 gtccgattgg tccaccgcga tgccgcgcgg gaaataaaac tgcccgtcgg cgctcccgaa
     2041 gctcccccac gacataatga attcgccggt catgtcgaac ttctggactc ggaagttggc
     2101 actatcaacg acgtagacgt agccacgcac ccgatcgatc gcaatgcccc agggagaatt
     2161 gaactggcct tcgccgttcc cccgcgatcc gaatttcatc aggtaaccgc ccagtttgcc
     2221 gtcgaacttc tgcacgcggt gattgttcgt gtccacgacc catacgtcgc ccttgccgtc
     2281 acaggccact ccgctgggat tgtggaaatt cgagttcgct gaaccgaagt tgccccagag
     2341 aatgatgaag ttcccggcgt tgtcgaattt ctgaacacga ttgttcccgt tatccacgac
     2401 gaagagggaa ccctgctggt ccacgcagag gccgtacatg ggagctatga actcgccacc
     2461 gtgcagcaat gaggccccac ggcctggacg cccccacttg gcgacgcaga catagcccga
     2521 cgtattgacc agaatagtcg agctggcggg cgtggaaacg ttcccgctga tatctttgaa
     2581 ccatacataa atgactttct gtccgtctcc cggcgacaga atgaaaggga tcgtcgctcc
     2641 gaacttgagc gccggcgtca catcaaccca tcccggtgtc cccgccatcg gcgtcatcgg
     2701 tgattcagag atgaagtagg ccccaacccc ggtatcgatg tcgtgggcgg aaatcgtgac
     2761 gacgacttcg ggcgaattcg tcatgaaggc gccgtgattg atgacggcgt agggattttg
     2821 cggagcggtg atatcgatca ggaccggtgt ggccgtgact tcactcgaca atgcgctctc
     2881 ggtttgatcc tggaacaccg ccgacacggc atagaagtag gccgtatcgt tcgtgagtcc
     2941 cgtatgcgta tagggattgg tcaccccttc gattctagtc gcggtctgct tcgtgatgcc
     3001 ctgcgacgtg ccgaagtaca gattgtatga cacagccccg ggcacctcca tccagctcag
     3061 aaacgtttcc gtatccccgg ccttcaccgc cacgctgtgc ggcggtggag tcgtactgac
     3121 agcctgtgac ttgggcgcgt cgtcttttcc ccggttcaac tcctcttccg tcggcacata
     3181 tttgaggacg cggttgttgc cgctgtcgac cacgtacaca cagccttctt tgtccacggc
     3241 cacgccgtac ggaaaattca attggccttc tgtcctgcca cgatttcccc atgaacagag
     3301 aaatgttcca ttgccgtcga atttctgaat gcggtgattt ccggtatcga ccacatagac
     3361 gttgcccaac gcatcacaga acacgcccca cggcgacttg aattgcccgg gaccgctccc
     3421 ctcccgtccc catttcgcca aaaaactgcc gcgcgcatcg aacttctgaa tacggttgtt
     3481 gccttcatcc gcgacgtaga tattgccgac aaaatcaacg gcgacaccgc gggggaaaaa
     3541 gaacgcaccg tcaaagcttc cgtcacgtcc ccacttcagc agtggcgtcc catccgcctt
     3601 gaacttctgg atgcgagcat tgctcgtatc cgatacatag aggttgccct cttggtcagt
     3661 cgtcagcccc cagggcacat cgaatctgcc catatcggcc ccgcgccagg ccatgccgaa
     3721 ttttccccag gcctgcatga cgttgccgtc gagatcaaac ctctgcaccc ggttgttgcc
     3781 gctgtcggcc acatacagca catcgtccga cccaatcgcg aggccgcgcg ggtaataaaa
     3841 cgatccttct tgagacgacg gctccgcgcc ccagcgagca aggaacttcc ccgtcttatc
     3901 gaatttctgg atggaatggt tgtccgtatc ggcaacgtaa atattgccgt ctttgtctag
     3961 agtgattccc gtgggtgagc tgaattctcc gtcatccact ccttcccgac cgatacacat
     4021 cgcgaggaga tacggcgcag gaatcgccat cgcttcctgc gattccggac tctccccttt
     4081 cgccgtcaca accgtgacga catagtggta acaggtcccg ttcgccagat cgtcatggat
     4141 gaagggtgaa ctggggcctt caatgcaggt cgccttctct ttcttcacac caatggtagc
     4201 cttgaagtcc tcttcgccgg cgatcggccg cgtgagctcg gaaaacttaa tctggactcc
     4261 tttggtcgtt tggaaataga cgttgtagta catcgcatcc ggaacagcgt cccaggtaag
     4321 tgtgattcga ccgttaccag gcttcgcctt cacattctcc ggcggtgggg gaagttcttc
     4381 cgcctcggca atcgaatcgc cacctcccca ttcatcctgt acagcgtcag cccgcaagac
     4441 ggaccgttta agccgaaaca tttcatcaaa caaccatgct ttgaccattg gtggtgcctc
     4501 gtgttgctaa gaccgctcag tgccaagaac tgagcgagtt tcaatgactt agaatcggat
     4561 tgaggagtct aacaaagcac tggaaagaga gtcaacgggg tcccataact tgaggcaccc
     4621 tatgaacgga aacgaaggtg cccgagagtg ggacttcgat caggagtgcc agagtagact
     4681 ttcccagctg ttaggtttgg ctgggtcacg cctgttcgac cggctctgtc agcaaagggc
     4741 tggccaaagc gagagttatg tcagaggatt ctgactcttc gcccagcatg gaacggtcca
     4801 tgtgtcccgt ctcttcctcc atcggcaaga acgcctgctc ggcatcaccg agttccttaa
     4861 attcacgtaa aggcggtagc tgggacagat cctgcaggcc gaaatgctca agaaagaact
     4921 tggtcgtgcc atacataatc ggccgaccag gcacttcctt gcggccgaca atgcggacca
     4981 gttttcgctc aaggagtgtg cggagaacac ccgacgtttc cacaccacgt atctgctcga
     5041 tttccgaacg gaccaggggc tgcttgtagg caatgatcgc cagagactcg agggcggacc
     5101 gagacaattt ctgcgccgtc ttggccttct caagcttctt gatccacggc gcatagtcgg
     5161 gtttggtcat caagcggtag ccgccagcca cctgcaccaa ctgcacgccg cggccgtctt
     5221 cttccagatc ctgtttcaac tgcgcgagcg cctgctccac gtccgtcttt ttgaccgccc
     5281 ccaagaccga taccaaacga gccaccggca caggatccga tgacacgaac aagaccgctt
     5341 cgagaatagc cttgagttcg cgccggtcga ccgcctcggc atgtccgttg ctattagaga
     5401 tgcccccttc cgcaacctca ccaccaacgc atgagatgtc ttccatcgcc tgggtcactg
     5461 tcaggctcat gctcccctcc attcctcatc atcgagctcg gccggatctg gcacgagcga
     5521 aaacgtacgc gagaccaaaa ttggaccgaa gttttctcct tgaaacaccc gtgctgttcg
     5581 caggcgaatc agttcgagca gcgcgaggaa cgtcacaatg atgaccattc ggtgactcga
     5641 cgattcgaag agcgaactga acgtgacgga ttcctgtccc tccagcgctt caaggatggc
     5701 gctcattcga tcccgcacgg tcaggttatc cgggatgatc tccagcaatt gcttgccggg
     5761 attgcgatcg aggattcccc tgagtgcatc aaccaaatcg aacaagttga tgtcctcgag
     5821 gagcgcctcc tctgattccg cttcgacagg cggactcggt tcgcggctgt agagctctcg
     5881 ccacaaccgc ccttggttat ccaactgacg cgctgcatcc ttgtattgtt tatattccag
     5941 cagccggcgg atcaattctt cccgtgggtc cggaccatcc tcttcatcct cagtcgtttc
     6001 atcgaccggc agcaacattt tggatttgat ctggagcaag gtcgcagcca taaccagaaa
     6061 ttctcccgcc acagtcaagt tcagatcctt catcgcctca agatattcca ggtattgtct
     6121 ggcgataagg gcgatcggga tgtcgtagat attgatttcg cttttcttga tgagatggag
     6181 caagagatcg agcggccctt cgaaattgtc gatgcgaacc tggtagggca gctccgtctg
     6241 ttccattccg tcgagctggt tatccacaag gcgctttata ccagctttgg tgggagcaga
     6301 gcaagccttg gcctattact tgtggagaat gtccgcagtg gggttatctt gatttgatga
     6361 gataggcgat cagtagagca ctgaatacca ggagggacag cgtgatgaca tattgaactt
     6421 tcgggctctc caaccccagc aggtctttct ccttaccggt tcgcttcgct ccagaaaatt
     6481 ccgggcctcc ttcgaagagc actttggcga aatcatctcg tccccgaaac tcggcattgg
     6541 aaaaatcggc cgtcgatcgg aaggtcgcac gccgaaaata tgcatcgccc tcaaaaacca
     6601 cgaatgggaa aaaggcttcc cgatcaaaga gcgactccga gaaatccgcc ataccgcgga
     6661 accggctgcc ggaaaagccg gtgccgagct tgaaatacgt ccgtgaaaaa tttgcgtccc
     6721 gctcaaactc gacctcaaga aattcggcga gtccgctgaa gcccgactgc tgaaaggtgg
     6781 cagcgccatg gaagcgagcc cgatgaaagc gggtatgcgg accaaaggcc gttttctcgg
     6841 cgaccacatc gttgagaaat cgagcctgca cgaaatagct ctctcgtaag aatatggcgc
     6901 cggataacag gaccggtcgc ataaacaccg accgcgagag atccactgtc tgttcgaagc
     6961 gcgttcctgt gaaagttacc ggacttctaa acacaagctg accttgcgcg gattgatgtc
     7021 gaatcgctcc gtacacgatc gaattgacga tggagatggc tccctgaacc acacggatgt
     7081 ctctactctc gatcgccgca acctccttga gatcccgagg catgggaccg gttgcaagca
     7141 catcgagcag caagtcaccg tgaatcatca cgccagagag atcaatatct ttgccgcgct
     7201 tgagcgcatc gatcacctgg attgcctcga cggcacgttt ctctcgttca ccatctgtgc
     7261 agtccgcact caatgagagc atcaacgcag cttcgctttg agttgctcca cttatgattt
     7321 cacaggacgc ggcgccccct tcaagcacac ttccccagat cgctgcaacc accgcaacga
     7381 gaccgatccg ctgccgaatg aacatgaccg cccttatacg aaatgccccg gtcgaaggca
     7441 aggcagaaac cgcgtccgcg ctggacgtca tgacatactt tgctagacta cgtaccggag
     7501 atcacgtgcc ttcgttttcg actttccctt gtcgcacgtg aacgtagtcc gatgcgtacc
     7561 aagctttcga tcatccgcgt cattctcgta agcctcgcat gcagtattcc gacggcactc
     7621 tttgcagaca acgtcatcga attgccggtc ttagccgccg tcgggataaa taaccagggt
     7681 gcgtttgaag tcctgttgat ttggtgggac aaaaagccgc aaccggatcc tccacagttg
     7741 cagtggatcg caggtcgggt ccgactggga tctactccct tgggttcgat gtctcaggct
     7801 ttctcctatg caatcgaaag aactccgtcc gtacagcaca gcgggactgt ctccgtggca
     7861 ggagtcgcct atgccccgac cggcaccgat ggcccgagcg ccggcgccgc catggccgtc
     7921 ggtttcatag ccctgttcaa aggggacaaa gttcagcgcg gtatcgctct gaccggcacg
     7981 ttcgagccgg gtggcaagat cggttttgtg gggagcattc cggataagct ccgtgccgcc
     8041 gcgcgtgaag ggtatcggac tattctggtt ccgcgcgggc acatctataa cgaacggtgg
     8101 aacctgaatg agttagctct cgagttgaac gtgtccgtca aggaggtcga gacgatcgac
     8161 gaagcctatc agctcatgac cggacagcgg atttagcgcg tgcccgccgg ctccccgatt
     8221 cctcgtacaa ttgcctcata ctccgtctca gtcagtcgat gcatgcccaa cgcaaggcgc
     8281 cgggtcagct ccgcctgatc gggaatctgc aacacggatt gcaggatcgc ctgactctcc
     8341 tgcggtgacg acacccaccg acgcaagatg ttcacccgga caaaaccaaa tcgggcttcg
     8401 gtttttaagt cgtctttgag aagctcgtca agatcacgga acggcaggac gagagaccgg
     8461 atctgaaact cggcgcagag gcccgccttg agcacataga caagtccgcg tgattggggc
     8521 tcgagatacg tctcaagatt tgtcttaatc agcgacgtgc agagacccca gaggccgtga
     8581 gagagttgta actctgcact gtcctccgag ctccgttccc gcaaggctcc cgcgatgaac
     8641 agaaaatgac tcatgacact cattcagggg aaaagaagaa accggatgga cgggcagact
     8701 cagtgtgtgc ggtagtcgtc ggaatcgtcg agcagatctt cggacttttc gatataatcc
     8761 acatcctcgt cttcgtcgtc cagtgagaga tcttcctcca tctcgtcatc catctcacct
     8821 tcaccggcga tctccaattc ttcctcgccc acttcctcgt cgagttcctc gtctttgggt
     8881 tccagtggga ctgccggttt tgcgatcact ttctgcctgg gggtttcctc ctcggcagat
     8941 ggcacgaact ttttagccgg cgctcctgca gccttcgttt gaacagttgg cttctttgcc
     9001 gccgctttct tcttcggaac agctttccgc ttcgacttgg gcgccgcttt ctttaccgcc
     9061 tttttcttca cagcagcttt tttgctcgcc ggtgccgagt gcgatttcgt tgcccgtttc
     9121 ttctttttgg ccatcgagta tctctccttc tgtcatcctc acatacaaga ggactcttcg
     9181 aggcaccatc tagcatgaac ttcgaggctc ttgcaacctg gtcagaatcc ctgcattagt
     9241 gccgggtttt gcacactagg gatcgaccca taccgaaatg attgccggcc ggctatcggc
     9301 tcccttca
//
LOCUS       DATDNW010000393         6040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_534074, whole genome shotgun sequence.
ACCESSION   DATDNW010000393 DATDNW010000000
VERSION     DATDNW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534074"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VI359_09340"
     CDS             complement(<1..488)
                     /locus_tag="VI359_09340"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY6974496.1"
                     /translation="MSRQILIVSAHPDDMEIGMGGTVAKLIEEGAEVTSFIVTDGGRA
                     SNPFGWTEQQMADERRAEALRAAEVLGVKDVIFCDQPDAADEVDVKAVGRKLVEVMTR
                     LKPTEVYTLHEEFDRHPGHRQAGRLVRESVGEAGLTPSGGVWAYEIWGPFPNWDRLEY
                     IDA"
     gene            815..1339
                     /locus_tag="VI359_09345"
     CDS             815..1339
                     /locus_tag="VI359_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974497.1"
                     /translation="MSWNTIYLHAGIASLLMLSGCSVAMALHGNQEPNFDAFEVGSTR
                     KQVEIQLGTPLSTTSRSDGTKEDTYKYEMGNSPNGARATLYFWYDLGTFGLAEPVFTL
                     IELFQGHDEVTKVVYSSDDRVLDIAGYIPVPPSPELKAALEAQAQHPKRPALAAEPSS
                     TPAGESQAVATRPH"
     gene            complement(1505..1879)
                     /locus_tag="VI359_09350"
     CDS             complement(1505..1879)
                     /locus_tag="VI359_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974498.1"
                     /translation="MSELSTLESLAEFWERVDYAGLFLVFVGVIVESVVEFTSLIKSS
                     LWKPKIGKISAFVLILGLALELIASSRLSVINRQVVGILSKEAAEAGKRAAHAEKATE
                     EERAARVKLETEVESERLSGKH"
     gene            complement(2605..4257)
                     /locus_tag="VI359_09355"
     CDS             complement(2605..4257)
                     /locus_tag="VI359_09355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY6974499.1"
                     /translation="MVRIREAREEDVGQIREIFLAVYGADYPHHEVYDELWLKRSVFT
                     DDALILVAEETATGKVIGTASVLFDFGAHSDLVGEFGRLAVHPDHRRVQVGKLLMDKR
                     LEAIQNRLHVGLVVARTVHPYAQRISLAQGFIATGFLPLKHFFRHRESFALLARYFGD
                     ALALRRNNPRIIPEAYALANLVMSQPPLMPDFIVDEDSAPYPAGGDYTIEQLQAEGYP
                     ALLRIERGRVRNREIFGPMRLDYGFFKLQARQTSYFLARSGGHIVGAVGYTMDPVEHT
                     VRVFELIALADDVVRFLLVELERKCRQEMGIEYIEIDVSAYAPRMQRTLLELNFLPVA
                     YVPAMVFDQVERLDIIKMVRLNKLQDLGPLVLVEPMQALADVVMRGFASCMIAPRMAQ
                     AIKEIPLFQGMNSEQATRLAGACTVREVRAGGRLFAEHDPADRLYLVLQGQVAITCGS
                     PAVMIGTVHAGETCGEVSLLSARPHSATATAEGHVEMAELLQGDLADLIRRRPDIGVI
                     IYRNLAVGLGDKLLRSSNSRRDRGLVESAVLHLASEATPATK"
     gene            complement(4277..5458)
                     /locus_tag="VI359_09360"
     CDS             complement(4277..5458)
                     /locus_tag="VI359_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY6974500.1"
                     /translation="MPLIPEPLKHRLVQLRRVLHQYPELSGQETNTAAVICKFLDGLA
                     IKYRAHVAGHGVVADIPGRAGVPCIVLRADTDALPIQEDTGLEFASVHDGVMHACGHD
                     GHTTMLLGAAALLSEEKDLPAPVRLIFQPAEEKGTGALDMIKAGALDGAGMIFGGHLD
                     RHYHPGNIVVAEGAVNASSDNFTVEIIGQGAHGARPHESIDAVVVGSLMIMALQTIVS
                     REVDPARPSVVSVGQFHAGTAPNVIAGQARLEGTVRAQDPAVRQQLLASVRRIAESIA
                     QLHGAKIQIIVKEGTPPLINTPEMASLARRAAVAAVGEANVLPLKTANMGAEDFSYYI
                     EKIPGAYVRFGSQVPGKEGFPAHSSKFDFDEEALAVGAAYYRAIAKIAGQRLSEHKPR
                     T"
BASE COUNT         1198 a         1814 c         1711 g         1317 t
ORIGIN      
        1 gcgtcgatgt actccaggcg atcccaattc gggaacggcc cccagatctc ataggcccag
       61 actcctcccg acggcgtgag ccccgcctca ccgacacttt ctcgcaccag ccggcccgcc
      121 tggcggtggc ccggatgccg atcgaattct tcatgcagcg tatagacctc ggtgggcttg
      181 aggcgagtca tgacttcgac cagcttccgc ccgactgcct tcacatcgac ctcgtccgcc
      241 gcgtccggct ggtcacagaa gatgacgtcc ttgacgccca ggacctccgc ggcccggagg
      301 gcttcagccc gccgttcgtc ggccatttgt tgctccgtcc aaccgaaggg attcgacgcc
      361 cggccgccat ccgtcacgat gaaagaggta acctcggcgc cttcctcaat cagtttggcg
      421 acggtgcctc ccatgccgat ttccatatca tcggggtggg cgctgacaat gaggatctgt
      481 cgactcatag gctcaacgtt gcacctggca ggtggaaatc ggagacaggc tactttttgc
      541 tcggtactgt gtcaactcag cgagatgacg gaatgcatgt attgccgggg tcgggtgctt
      601 gagatctacc ttcaacctca ccggcgtttc gctggatcgc ttttgatttt gcatttacca
      661 attttggttc acttcttccc gatcgactct cctcaccacc cagaagttat tgaaatgtct
      721 gattaggaat ttcctatgct cccggcatcc ttcttgcgga cctagatgtg acatagggca
      781 tgagccctct caccaggcac cgcaggaggt ggttatgtct tggaacacta tttatctaca
      841 tgcggggatc gcttcgctcc tgatgctgtc tggatgctcc gtcgccatgg cgttacacgg
      901 gaaccaagaa cccaactttg acgcattcga ggtcgggtca acgcgaaaac aggtggagat
      961 tcagcttggg actccattgt ccacgacctc acgttcggac ggcaccaaag aagacaccta
     1021 caagtatgag atggggaact cgcccaatgg ggctcgcgcg acgctctatt tttggtatga
     1081 cctcggtacg tttggcctgg cggaacctgt attcacgctc atcgaactct ttcaaggaca
     1141 cgatgaagtc acgaaagtcg tgtacagttc agatgatcgt gtgctcgata ttgcgggata
     1201 tattcctgtt cctccgtcac cagaactcaa agctgcccta gaggcccaag cacaacaccc
     1261 gaagcggcca gcactcgcag cggagccttc ctctactccc gctggcgaga gtcaagccgt
     1321 tgcaacgcgg cctcattagc ggtcacgtgc tcactcaaca gggcccgcag gaggacaatc
     1381 ctgcgggcct tgctctcaat gctcccaccg agagcagggg accatacagg cggcgtttca
     1441 tgtacgtgat gagtccgtcg tccctttcgc catccgctca cccaaccgaa tgagcgtctg
     1501 actcttaatg tttgccgctc agacgttcgg actccacttc cgtttctaac ttcactcgtg
     1561 cggctcgttc ttcttctgtc gctttctccg catgcgctgc gcgttttccg gcctctgccg
     1621 cttccttgga taatatgccc accacctgcc ggttaatgac ggacagtctg gaggatgcaa
     1681 tcaattcaag cgccaaacca aggatcaaca cgaaggctga tatttttcca attttcggtt
     1741 tccacaagga cgacttgatc aggctggtaa attccaccac cgattccacg atgactccca
     1801 cgaagaccag aaacaagcct gcatagtcga cacgctccca aaattccgcc aatgactcta
     1861 gcgtcgagag ttctgacatc tgttcctcac atcgttttgt gcgatttccg gcttgaatcg
     1921 tgttggcaca acaattgacc aatcacagtt tctgtggata aacctgtgaa caacgttcgc
     1981 tttgagcgcc gaataggcct gtatcaccta tcgaatcgca aactgccttc tttttgggca
     2041 ttgggcctca tgaaccagtg ccccaatatc ttggggtcag acgagatgtc tatggaatta
     2101 gacatatatc tttcttgact tttcgtatcc gacggacctg agagccttct tgtgccgcat
     2161 tgcttaggga tatccctagg tcacctaata gctatccaca gctacgggtt cttcctgagg
     2221 ataattgtcc cttgaaacgc ctaaatagca ggaccagtgc ggcaatcgct caccctattg
     2281 gagatggtcg cctcatcgag ggacgcggtt tcacatttgg ggcgactggt gctcatggat
     2341 gatccgactt cgatctctcc tgtccagatg gcacagagtc gaacaaataa tctattgggc
     2401 gagacaacca ggacattctc cgtttcagca ggttggaatg agaatttcgc gacaggctac
     2461 gactcgctgg agtttgtgtc aacctcacga aatcgcggaa tacaatcatt ggccaggctc
     2521 ggcgcagtca gtcacgcgct ccacacaaca aggcccgcag gtctcccctc ctagggtgat
     2581 gcgggaatcg agttgatccg gttcttactt tgttgccgga gtggcttcgg atgcgaggtg
     2641 cagcactgcc gactcgacca gccctcggtc tcgccgtgaa ttgctcgagc gtaggagctt
     2701 gtcacccaat ccgactgcca agttgcggta gatgatgacg ccgatgtcgg gacgccggcg
     2761 aatgagatct gccagatcgc cctgaagcag ttcagccatc tccacgtgac cctcggccgt
     2821 cgccgtggct gagtggggac gggcggagag gagcgacact tctccgcagg tctctcctgc
     2881 gtggacagtc ccaatcatga cagcagggct tccgcacgta atggctactt gtccttgcaa
     2941 cacgagatag agccggtccg ccggatcgtg ttcggcgaaa agccgcccgc ccgcccgaac
     3001 ttcccgtacc gtgcaggctc cggcaagcct ggtcgcctgc tcgctgttca tcccctgaaa
     3061 caacggaatc tccttgatgg cctgtgccat gcgcggggca atcatacagc tggcaaagcc
     3121 gcgcatgacg acgtcggcga gggcctgcat cggttccacc aacacgagcg gccccaaatc
     3181 ttgcagcttg ttcaatcgca ccatcttgat gatatcgagc cgttccactt gatcaaagac
     3241 catggccggc acataggcga ccggaagaaa attcagctcc aggagggttc gttgcatccg
     3301 cggggcgtag gcgctgacgt cgatctcgat gtattcgatg cccatctcct gccggcactt
     3361 ccgttctaac tcaacaagga gaaaccgcac gacgtcgtcg gccagggcga tcagctcgaa
     3421 cacgcggacg gtgtgctcga cgggatccat cgtgtaaccg acggctccga cgatgtggcc
     3481 gcctgagcgg gccagaaagt aactggtttg cctcgcctgg agcttgaaga atccgtagtc
     3541 cagccgcatg gggccgaaga tttcccgatt gcgcacccgg ccccgttcaa tgcgcagcag
     3601 agccggatac ccttcggcct gaagttgttc gatcgtgtag tctcccccgg cggggtacgg
     3661 tgcggaatct tcgtcgacga tgaaatccgg catgagcggc ggctggctca tcacgagatt
     3721 ggctaaggca taggcctcgg gaatgatccg tggattgttg cggcgcaacg ccagcgcgtc
     3781 gccgaaatac cgggccagca gggcgaagct ctcgcggtgg cggaagaaat gcttcagagg
     3841 aaggaagccg gtcgcaatga acccttgcgc gagactgatg cgctgggcat aggggtgcac
     3901 ggtgcgcgcg acgaccaggc cgacatgtaa gcggttctga atggcttcga gccgcttgtc
     3961 catgagtagc ttgccgacct gcacccggcg gtggtccgga tggaccgcga ggcggccgaa
     4021 ctctccgaca agatcggaat gggccccgaa gtcgaagagc acggaagccg tgccgatcac
     4081 tttgcccgtg gcagtctctt cggcgacgag aatcagcgcg tcgtccgtga agacggagcg
     4141 cttcagccag agttcgtcgt agacctcgtg atggggatag tcggcgccgt agacggcgag
     4201 aaagatctcg cggatctgac cgacatcttc ttcgcgggct tctctgatcc gaaccatgat
     4261 agccctgtgc gcgtggtcaa gtgcggggct tgtgctcgct gagccgctgg cctgcgatct
     4321 tggcaatggc ccggtaatag gccgcgccga cggccaaggc ctcttcgtcg aaatcgaact
     4381 tactggagtg ggcgggaaag ccttccttcc ccggcacctg actcccgaac cggacatagg
     4441 ccccgggaat tttttcgatg tagtagctga agtcctccgc acccatattg gccgtcttga
     4501 gcggcaacac gttggcctcc cccactgctg cgaccgcggc gcgtcgcgcg aggctcgcca
     4561 tttctggcgt gttgatgagc ggcggcgtgc cttctttcac gatgatctgg atcttggcgc
     4621 catgcagttg ggcaatcgat tcggcgatgc ggcggacgga ggcgaggagt tgttgccgga
     4681 cagcaggatc ctgcgcacga accgtcccct ccagcctggc ttgtcctgcg atgacgttcg
     4741 gcgcggtccc tgcgtggaac tgccccacgg acaccaccga cggacgcgcc ggatcgactt
     4801 cgcgggagac gatcgtttgc aacgccatga tcatcagact cccgactacg acggcgtcga
     4861 tgctttcgtg cggccgggct ccgtgcgcac cttgcccgat aatctcaacc gtgaagttat
     4921 cggaggacgc gttgacagcg ccttcagcca cgacgatgtt ccccggatgg tagtggcgat
     4981 cgagatggcc gccgaaaatc atgccggcgc catccagcgc tccagccttg atcatgtcca
     5041 gcgcgcccgt gcctttttcc tctgccggct gaaaaatcaa tcgcacggga gccggcaagt
     5101 ctttctcttc tgagaggagt gccgctgcgc cgagcaacat cgtggtgtgc ccgtcgtggc
     5161 cgcaggcatg catgacgccg tcgtggaccg atgcgaattc caagccggta tcttcctgga
     5221 tcggcagcgc gtccgtatcg gcacgcaaca cgatgcaggg cactccggcg cgtccgggaa
     5281 tgtcggcgac gactccgtgg ccggcgacgt gggcccggta cttgatggcg agcccatcga
     5341 gaaatttgca gatgaccgcg gcggtattcg tctcctgtcc gctcagttcg ggatactgat
     5401 ggagcactcg tcgaagctga acgagacggt gtttgagcgg ttcaggaatc aatggcatga
     5461 ttgcaacctt ggcagttaac gtacgacggc ctgtaggcgt acccttcgcc gggtccggcc
     5521 agaccggcga tctgacgata acagcgctat gctagcacag acccgaccac ggtttctgca
     5581 gcgggattgc cactgcttga atggtgctct tgctatgctc gctcgtctcc caggtagggt
     5641 gacctggtcg accctggagt gcgcacgtcg aacgaccact aatggtccct ccaagcttgc
     5701 tcgctctctt tcctacaagg gtggcctgga tgagtcccca actgcgcacg cttaatgttc
     5761 cttcaaagct tgctcgtatt tcacttcaat ggagtaacgc ctgtgctgct cctactgcgg
     5821 ccgtcgaggg agcaccgccc ctaatataga aatctgatta atctcgcgtg cgctctccgg
     5881 gagcaaagga gcagcatggg cgttgttccc tatcattttt atcgtggggg ttccgtgagc
     5941 acaggggact aaccaggcta cccttcccag cgcctcattg atatgatggg aaccgattgg
     6001 aggtcgtcgt ggctgccgca gcatccccgc cctctgtgag
//
LOCUS       DATDNW010000394         3022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_133310, whole genome shotgun sequence.
ACCESSION   DATDNW010000394 DATDNW010000000
VERSION     DATDNW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133310"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VI359_09365"
     CDS             complement(<1..252)
                     /locus_tag="VI359_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974501.1"
                     /translation="MPTKRAPSLVHLEQALREKRREVDVLHRITDSINNTLDLEAVLK
                     HIVEVVVEVTKADACLLYLLSEGRDELILRASKNPHPKLI"
     gene            complement(260..1429)
                     /gene="aroB"
                     /locus_tag="VI359_09370"
     CDS             complement(260..1429)
                     /gene="aroB"
                     /locus_tag="VI359_09370"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY6974502.1"
                     /translation="MRKIPEQIVAVALGDRSYDIAIQPGLLTEVGERLTNLAQSRQVG
                     VVTDRHVARHHLPAMLRSLRSAGFDPTDIVLAPGERTKTLSTVGAILDVLVKKKFERK
                     SLLVALGGGVVGDLTGFAAAIYQRGIPFVQVPTTLVAQVDSSVGGKTGVDHPLGKNLF
                     GAFHQPRAVFVDPVTLQTLPHREWVAGIAEVIKYGVIADEAFFSFLEQSIGSLLQLDE
                     LTVLRVIARSCEIKAQVVADDERESDRRRILNYGHTIGHALESLEGYRRMIHGEAVGI
                     GLVQEADLAHHLGICEREVVERIRNLVGCAGLAEAMPSVSFQSLWAAMQHDKKVVGGQ
                     VVGVWPVRIGEVVIRPLDQRACEDWYHSRQGRTSRLEPHRRSTRRRSGIRNRKRR"
     gene            complement(1871..>3022)
                     /gene="pilQ"
                     /locus_tag="VI359_09375"
     CDS             complement(1871..>3022)
                     /gene="pilQ"
                     /locus_tag="VI359_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY6974503.1"
                     /translation="KSKVTMKLVSVPWDQALDMILKMNGLGKIKQGNILWVDSLANIA
                     KQQDEEARAKDAKTKAEELVDRVFYIRNLQAQELMTALTRYLSPRGVMQQSQGSNALI
                     VRDTETRVGTLKQLIEGLDLEVPQVQIEARIVQADTVYARGLGIQWGITNTNFTSNSF
                     NTVGNITGPYVPGSGTAPTSIPTDFIVNLPAQVAGLPAVPSIGYTFGKLPLGFTLDLR
                     LSAGELLGLSKVIAAPKIVTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVT
                     PQITSRDPKETGKLIMMRVRATRNAVGARSNPAGPSIDRREATTQVNIRDGETMVIGG
                     VFIDTQNNNVQGIPYLSRIPVLGWLFKNKSESVSKQELLIFLTPQIVKT"
BASE COUNT          655 a          871 c          759 g          737 t
ORIGIN      
        1 gattaacttg ggatggggat tcttcgaagc ccgcaggatc aactcatctc gaccctcaga
       61 caggagatac aggaggcagg catcagcttt ggtcacctct accaccactt ccacaatgtg
      121 tttcaacacc gcttcaagat cgagtgtgtt gttaatcgaa tcggtaatgc gatgcaacac
      181 atcgacctct cgcctttttt ctcgcagagc ctgctcaaga tggacaaggg agggtgcgcg
      241 tttcgtgggc attctgtcgt tacctgcgct tcctgtttct gataccggaa cggcgccttg
      301 ttgaccggcg atgaggctcg aggcggctgg tacgtccctg acggctgtga taccagtctt
      361 cgcaggctcg ttggtccaac ggccgaatca cgacctctcc gatccgcacc ggccataccc
      421 cgacgacctg tccacccact actttcttgt cgtgctgcat ggcagcccag agtgattgga
      481 acgacacgct cggcatcgct tctgcgagac cggcgcaacc gaccaaattt ctgatcctct
      541 cgaccacttc acgttcgcaa attcccaaat gatgagcgag atccgcttct tgaacgagcc
      601 cgatccctac cgcttcgcca tgaatcatcc ggcgataccc ctcaagggat tccagcgcat
      661 gaccgatcgt gtgaccataa ttgagaatgc gccgtcggtc cgattctcgc tcgtcgtccg
      721 caacgacttg cgctttaatt tcgcacgacc gcgcgatcac ccgtaaaacc gtcagctcgt
      781 cgagttgaag caatgaaccg atcgattgtt ccagaaacga aaaaaacgct tcgtctgcga
      841 tcacgccgta cttgatgacc tcagcaatcc cagctaccca ttcgcggtgt ggcagtgttt
      901 gtagcgtgac gggatcgacg aagacggctc gcggctggtg aaacgcgccg aagagattct
      961 ttcccaatgg gtgatcgacc cccgttttcc cccccacact ggaatccact tgggcaacca
     1021 acgtcgtcgg gacctgcaca aagggaatgc cccgctgata gattgccgct gcaaagccgg
     1081 tgaggtcgcc gacaactcca ccgccgagtg cgactaggag tgattttcgt tcgaatttct
     1141 tcttgaccag cacatcgagg atggctccca ccgtcgacaa ggtctttgtt cgttcgcctg
     1201 gcgctagcac aatatcagtc ggatcgaacc ccgcacttct cagcgatcgg agcatcgccg
     1261 gtaggtggtg ccgagccacg tgacgatccg tgaccactcc gacttgacgg ctttgcgcta
     1321 gattcgtgag gcgctcaccc acttccgtca gaagacccgg ttgaatggcg atgtcgtagc
     1381 ttctatctcc taacgccacg gcgacgattt gctcaggtat ttttctcata ctcgacacac
     1441 gagaattatg gatgtcacat tgcaacaatc ttcacaccca ttccctcggc cggatggcgc
     1501 ggggatgttc accttttcag gcgattcact gatcctgcgt tacaggagga gcaagaaccg
     1561 gggggatggt agcacaacgg tgcgaaaact aaaacgattt tggtgagcct cgccagagag
     1621 attatcgttg tcccacttag cttgcacaag gcactggcgc tttgctggtg gttaacattt
     1681 cccaaaccca gcgcgtgaat tcagacagag cggcgaagcc cttcgcctat ttggcgaagg
     1741 gcctaagtca gcattgactg tgttgtgagc aatcagaagc gctggagggt atgggtgtga
     1801 gtcctggcaa cactcgcttg gattcactct tgtcttaatg cagcatcttg aaaaccccga
     1861 accgacatgc ctatgttttt acgatttgag gcgtcaagaa gatcaagagc tcttgtttcg
     1921 acacagactc agacttgttc ttaaacaacc atccaagaac agggatgcga gacaagtatg
     1981 gaatcccttg cacgttgttg ttctgcgtgt caataaacac gccgcctatc accatggttt
     2041 ccccgtctcg aatatttacc tgcgttgtcg cttcccgccg atcgatactc gggccggccg
     2101 ggttacttct cgcaccaacc gcattccgtg tggcccggac cctcatcatg atgagcttgc
     2161 cggtttcctt cggatcccgg gacgtaattt gtggcgtaac attcagttca agattggcat
     2221 ccacgaaggt ggtctgagtg ccttgtaacg aggttgtctg aaagggaatc gactcgccct
     2281 gcgaaatctt cgcttcccgt ttgtccagtg tgacgatttt tggagccgcg ataaccttcg
     2341 acaatccgag caactcgccg gcagaaagcc gcaggtctaa cgtgaatccc agcggtaact
     2401 tcccaaatgt atagccaatt gacggtacgg caggcaagcc cgcgacctgg gcgggcaagt
     2461 tcacaatgaa atctgttggt attgatgtgg gcgctgttcc tgatccggga acatatggcc
     2521 ctgtaatatt tcctacagtg ttgaagctat tggaagtgaa gttggtattc gtgatccccc
     2581 attgaatccc gagccctcgg gcatagacgg tatctgcctg gacgatcctc gcttcaattt
     2641 gaacctgtgg cacttcgaga tcgagacctt cgatgagctg tttgagcgtt ccaaccctgg
     2701 tctcggtatc acggacgatc aatgcattgc ttccttgact ctgctgcatc acgcctcgag
     2761 ggctcaaata tcgggtcaag gccgtcatga gttcctgcgc ctgcagattc ctgatataga
     2821 agacccgatc aaccaactct tccgctttcg tcttggcgtc cttcgctcgc gcctcttcat
     2881 cctgctgctt ggcgatattt gccaatgaat cgacccaaag aatgttcccc tgcttgatct
     2941 ttcccagacc attcattttc aaaatcatgt cgagcgcctg gtcccagggt acactcacca
     3001 gcttcatcgt gaccttgctc tt
//
LOCUS       DATDNW010000395         7625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_677885, whole genome shotgun sequence.
ACCESSION   DATDNW010000395 DATDNW010000000
VERSION     DATDNW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677885"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VI359_09380"
     CDS             complement(<1..837)
                     /locus_tag="VI359_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY6974504.1"
                     /translation="MSTATGLEGSSDSLSVTLAEQRRAIGLRLIEGASGAETMAAMTD
                     LMDDLITGCYRTAVRQGGEELASIGLRQCCVVALGGYGRRELAPYSDIDVMVLFRPEI
                     GPRVQPLVQAVLRPLWDSGCQVGHSVRTIGDCIELASSDSTVKTSMMEARFLAGSADL
                     FQEFHGRYLRKVVTQATDTFLDLKLEERRREYEKFGETVYLLEPNVKKSKGGLRDLHL
                     LQWVGMARYHTPTIRELSDRGILSRADYLALVEAREFLWRVRSLLHVHGGMAQEILTF
                     DEQ"
     gene            complement(858..1196)
                     /locus_tag="VI359_09385"
     CDS             complement(858..1196)
                     /locus_tag="VI359_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY6974505.1"
                     /translation="MKLVEAIIKPFKLDEVKDALLEIGVQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKVEVAVQDNQVPRVLETITRAAKTGSIGDGKIFVRDLHAAVRI
                     RTGETGETAL"
     gene            complement(1300..2631)
                     /locus_tag="VI359_09390"
     CDS             complement(1300..2631)
                     /locus_tag="VI359_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY6974506.1"
                     /translation="MTSLHRSWLWGSIVLMSLILWATIVSAEDVSPRLAPGGINSGDT
                     AWMLAASALVLGMIIPGMVFFYGGLVRSKNVIGTMVQAFAILCVVSVLWVICGYSLAF
                     GPDRGGVIGGLDWVGLSGVGAMPHPVYAPTVPHEAFMLFQMLFAAFTPVLIAGAFAER
                     MKFGAAVLFAALWSLFVYVPLAHWVWGGGWLSKLGVLDFAGGTVVHISSGVSALACAM
                     VLGKRRGWRTDYMAPHNLPFVLLGTGMLWLGWFGFNGGSARGANAVAIGAVTVTHVTA
                     ASAALAWMIIEWQHRGKPTVLGIASGAVAGLATSTAGAGYLGPGSAIIVGIAAGLCSY
                     AAIVWKGKIGYDDSLDVMGTHGVGGILGTLAVGLFASKAVNPSASDGLFFGNSGQFGI
                     QIVAVTATVIFAFVGTYLILKLVEGGMGLRVSTEEEATGLDLTQHNERAYS"
     gene            complement(2642..4435)
                     /locus_tag="VI359_09395"
     CDS             complement(2642..4435)
                     /locus_tag="VI359_09395"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY6974507.1"
                     /translation="MRMFRIAMVQMNPTVGDLEGNVRRIISWLREARKARPDLVAFPE
                     LVITGYPPEDLLLKPQFIEDNRRALDEVIRACRDLVAVVGYVGQAGLSDEKPSRSAVV
                     SAGRHELYNAAAVIADRRLAASYRKWYLPNYGVFDESRYFHPGRRLPLIAVNGTMVGV
                     NICEDIWLPEGPTRAQAASGAEVIVNINASPFHVGKSRSREQMLATRARDNGVIVTYT
                     NTVGGQDELVFDGNSVILDQTGEVIARGGAFREELIVADLNVEAVARGRMAHGRRKNV
                     YGKVAAAVDRVAVKVSTTTAKRTRAVPPLTVPLAEIDEVYHALVLGVKDYVRKNGFKR
                     VVIGLSGGIDSALTCVIAVDALGPENVLGVFMPSPYTSPESGEDVADLVHRLHIELKT
                     VPITPTFDSYLRSLAASFEGRPQDTTEENLQARIRGNILMALSNKFGHLVLTTGNKSE
                     MSVGYATLYGDMAGGFAVIKDVPKTMVYELAALRNRRDGTAVIPKRILDRPPTAELRP
                     NQKDEDSLPPYALLDPILKAYVEEDRSVDEIIAMGYERATVLRVVALVDRSEYKRRQA
                     PIGIKITHRGLGKDRRMPITNGYRGIESSGS"
     gene            complement(4763..5638)
                     /locus_tag="VI359_09400"
     CDS             complement(4763..5638)
                     /locus_tag="VI359_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY6974508.1"
                     /translation="MPRPRKIPAVAPTASVGRVGRLALEFVQQNNRTVFGRTSCQSPW
                     HLFPPIYLDETGSAYTLLNNPSGGLVGGDHLSIDLAVGPKAHVLISTPSANRVYRSLS
                     ENSVQEIAIRVGPEGILEWVPEHTIPFAGSRFRQTIDVKLAAGASLILWDAIASGRIA
                     RGERWKFTSFQNDIRITTASGTSVGERYALTPADQSGGHGLAEEWDYVGSLFVIGDGI
                     DTQVWTSLETALAEILEEHPGQVLGGVSQPAAPGLVVKLVARSAPVLITVLNALWGAV
                     RRGLWNLPPAILRKY"
     gene            complement(5658..6329)
                     /gene="ureG"
                     /locus_tag="VI359_09405"
     CDS             complement(5658..6329)
                     /gene="ureG"
                     /locus_tag="VI359_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY6974509.1"
                     /translation="MHRADEHHCGSGRVTAARAKHIPVIGIGGPVGSGKTALVEALCR
                     KLRDRYSLAVVTNDIFTKEDAEFLTRRGALPQDRILGVETGGCPHTAIREDASHNQEA
                     LDELLRRHPDVELMFVESGGDNLAATFSPELVDHTIYVIDVAEGDKIPRKGGPGITRS
                     DLLVINKIDLAPYVGADLGVMDRDSRRMRGDRPFIFTNLMSGEGLDRVVSWIEPHIHQ
                     HAHRG"
     gene            complement(6351..7037)
                     /locus_tag="VI359_09410"
     CDS             complement(6351..7037)
                     /locus_tag="VI359_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEY6974510.1"
                     /translation="MDTLSLLNGVRFIDSFFPSGGYAYSSGLEAAVKGGSVRDGKDLA
                     HYVEDLLLHGVSRREAVAVAIAHQAVTTGKLQPALKVDCELDTFKIGRDSRLASRQMG
                     RQIVKVSAEQIQGCRLLRDYRTAVDEGRTPGHVPVSMGLTLAACGWSRQNTVAAYLYQ
                     TAQGFVSAAMKLLPVGQQEGQHLLRQWVPLIANLSKAAKPQEVLISWAPVHDIYAMRH
                     ARLESRLFRS"
     gene            complement(7252..>7625)
                     /locus_tag="VI359_09415"
     CDS             complement(7252..>7625)
                     /locus_tag="VI359_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY6974511.1"
                     /translation="EMVLKSGFIAQAQMGDPNASIPTPEPIISRPMFGAFGRALSSTC
                     ITFLSQVGLDREIPKKLGLQKRVAAAKNCRSVKKRDLKLNDYLPKIEVDPETYVVTAD
                     GVRLTCEPAVVLPMAQRYFLF"
BASE COUNT         1616 a         2439 c         2080 g         1490 t
ORIGIN      
        1 ctgctcgtcg aaggtcagaa tttcctgcgc cattcccccg tgcacatgga gcagtgagcg
       61 aacccgccag aggaattccc gcgcctcgac caaagcaaga tagtcggcac gagagagaat
      121 gccccggtcc gatagctccc gaatcgtcgg cgtatgatag cgcgccatcc ccacccactg
      181 aagcaggtga agatcacgaa gcccaccctt gcttttcttc acgttcggct cgaggagata
      241 gaccgtctca ccgaactttt catattcgcg tcgacgttcc tcgagcttga gatccagaaa
      301 ggtatccgtc gcctgtgtca cgaccttccg caggtagcga ccgtgaaatt cctggaagag
      361 gtccgcactc cctgccagga accgggcctc catcatcgac gtttttaccg tcgagtccga
      421 actagccagt tcgatacagt cgccaatcgt tcgaacgctg tggcccacct ggcagccgct
      481 atcccagagc ggacgcagaa cggcctgaac gagcggctgg acgcgcggtc cgatctccgg
      541 ccgaaacaag accatcacgt cgatgtcgga gtagggtgcc aactcgcggc ggccataccc
      601 gcccagggcg accacacaac actgtcgaag tccgatcgac gccagttcct ctccgccctg
      661 gcggacggcg gtccgataac agccggtaat caggtcgtcc atgagatcgg tcatcgccgc
      721 catggtctct gctcccgagg cgccctcgat caggcgaaga ccgatggcac ggcgctgctc
      781 cgccagagtg acggagaggc tgtcactgct tccttcgaga ccagttgcgg tggacatgcg
      841 atcgttcggc gacatggtca caacgcggtt tcccccgtct cgcctgttct aatacgaacc
      901 gctgcgtgca aatcgcggac gaagattttt ccatctccga tgctgccggt cttcgccgcg
      961 cgagtaatgg tttccaagac ccgcgggacc tgattatcct gcaccgccac ttcgactttg
     1021 accttgggaa cgaattcgat cgtgtattcc tgtccgcgat aggtctcttt gtgacctttt
     1081 tggcggccga agcctttgac ttcggtgacc gtcatgcctt gcacaccgat ctcgagcaag
     1141 gcgtccttca cttcatcaag tttgaacggc ttaataatgg cttcaactag tttcatatct
     1201 gctcctcatg ggctcggggc gagaggctcg tggcaagtgg aaaagaggac atgttcattc
     1261 cgacccctag ccccacgcct cgcgccactt gcccattgat tatgaatacg cacgctcgtt
     1321 atgttgcgtc aaatccagac ctgtggcttc ctcttccgtc gagacccgca gtcccatccc
     1381 gccctcgacc aacttcagaa tcaagtacgt cccgacaaag gcaaaaatga cagtcgccgt
     1441 caccgccacg atctgaattc cgaactgacc ggaatttccg aagaacagtc cgtctgatgc
     1501 ggaaggattc acggctttcg aagcaaagag cccgaccgcg agggtcccaa gaattccgcc
     1561 gacgccatgc gttcccatga cgtcaagact gtcatcatag ccgatcttcc ccttccaaac
     1621 aattgcggcg taggagcaca gaccggccgc aatgcccaca attatggccg accccggacc
     1681 aagataaccg gctcccgccg tggacgtggc cagtccggcc actgctccgc tggcaattcc
     1741 aagcaccgtc ggcttccccc ggtgttgcca ttcgatgatc atccacgcca gtgccgcaga
     1801 cgccgcggtc acatgcgtca ccgtcacggc accgatcgcc acggcgttcg ccccccgcgc
     1861 actgccaccg ttaaaaccga accaccccaa ccacagcatc cccgtcccca ggagaacgaa
     1921 cggaagattg tgcggcgcca tatagtcggt tcgccagcct cgacgtttgc ccaataccat
     1981 ggcgcaggcc aatgcactca ccccggagct gatgtgcacc accgtgcccc cggcaaaatc
     2041 gagtacaccc agcttgctga gccaccctcc tccccagacc cagtgtgcca atggcacata
     2101 gacgaacagc gaccagaggg ccgcaaacaa cacagcagcc ccgaatttca tccgttcggc
     2161 aaacgctccg gcgatcagca ccggcgtaaa cgcggcaaag agcatttgaa acagcatgaa
     2221 ggcttcgtgc gggacagtcg gagcatagac cggatgcggc attgcgccaa caccggataa
     2281 tccgacccaa tcgagcccgc cgatcacgcc gccacgatcc ggtccgaacg ccagactgta
     2341 gccacagatt acccagagca cgctcacgac gcagagaatg gcgaacgcct ggaccatggt
     2401 cccgatcaca ttcttgctgc gaaccaatcc tccgtaaaag aacaccatgc cggggatgat
     2461 catgcccagc accaacgccg aggccgccaa catccaggcc gtatcgccgc tgttgatgcc
     2521 acctggagcc agacgtgggc tcacgtcttc ggcagagaca atagttgccc aaaggattag
     2581 actcatgagc acgatactgc cccacagcca agaccgatgc aggcttgtca tcagagcctc
     2641 ctcatgatcc gcttgattcg atgcctcgat agccgttcgt gatcggcatg cgccgatctt
     2701 tgcccaagcc gcgatgcgtg atcttgattc cgatcggcgc ctgcctccgt ttatactcgc
     2761 tgcgatccac caacgcgacg acgcgcagaa ccgtggctcg ctcgtatccc atcgcgatga
     2821 tttcatcgac cgaccgatct tcctcgacgt acgccttgag aatcgggtcg agcaaggcgt
     2881 agggcgggag actgtcttcg tccttctgat tgggacggag ttctgccgtc ggcggtcggt
     2941 ccagaatgcg cttgggaatc acggccgtcc cgtcccggcg attgcgaagt gcggccaatt
     3001 catagaccat cgtcttgggc acatccttga tcacggcaaa cccgcctgcc atgtccccgt
     3061 acagcgttgc atagccgacg ctcatttcgc tcttgttccc cgtggtaaga accagatggc
     3121 cgaatttatt cgacagggcc atgaggatgt tcccccggat gcgggcttgg aggttttcct
     3181 cggtcgtatc ctgcggcctt ccttcgaacg acgcggccaa cgaccgaaga taggaatcga
     3241 acgtcggcgt aatcggcacg gttttcaatt cgatgtggag gcggtgcacc aggtccgcaa
     3301 catcttcccc gctttccggt gaggtatagg gcgacggcat gaacaccccg aggacattct
     3361 ccggaccgag cgcatcgaca gcgatgacgc acgtcaacgc ggaatcgatg ccgccgctga
     3421 gaccgatgac gacacgtttg aacccgttct ttcgtacata atccttcacg cccaatacca
     3481 gcgcatgata gacctcgtcg atctcggcca acgggacggt aagcggaggg acggcgcgag
     3541 ttcgtttggc cgttgtggtg gagactttca cggcgacgcg atcgacggct gccgcgactt
     3601 tcccgtacac gttctttcgg cgtccatgcg ccatacggcc ccgcgcgacc gcttcgacat
     3661 tcagatcggc cacgatcagt tcttcccgaa atgccccgcc tcgcgcaatg acctcgccgg
     3721 tctgatcgag gatcacgctg ttgccgtcga acaccagttc atcctgccct cccaccgtgt
     3781 tcgtataggt cacgatgacg ccattatccc gcgctctggt cgccagcatc tgttcccgcg
     3841 aacggctttt cccaacgtgg aacggcgagg cgttgatgtt gacgatgacc tctgcaccgc
     3901 ttgccgcctg cgcgcgggtc ggtccttccg gcaaccaaat gtcctcgcag atatttacac
     3961 cgaccatcgt gccgtttacg gcgatcaacg gcagccgccg tccaggatgg aagtagcggc
     4021 tctcatcaaa cacgccgtag ttcggcaggt accacttgcg atagctcgct gcgagtcgcc
     4081 ggtcggcgat cacggccgct gcgttgtaca gctcatgtcg tccggccgac accaccgccg
     4141 accgggaggg tttttcatcg gagagccccg cttggccgac atacccgacg accgcgacaa
     4201 gatcgcgaca ggcccgtatg acctcatcca atgcacgccg gttgtcctcg atgaattgcg
     4261 gcttcagcag caggtcctcc ggtggatagc cggtaatcac cagctcggga aatgcgacca
     4321 gatccggcct ggcctttcgg gcctcgcgta accaactgat gattcggcgg acgttcccct
     4381 ccagatcccc gacggtggga ttcatttgaa ccatggcgat gcgaaacata cgcataagcc
     4441 tctaccgtga ctgtctgaag gatgggatgc tcaaacaaac gattcggcta ggccgcaggg
     4501 aagctcggac accgaggcgt actgcattga cgtacgtcga ggtgggtgag cgaccgagaa
     4561 cgacgccgag cgtttgtttc agcatcccat ggtaaaaaaa aacgccctca atcccaagag
     4621 aggaccgagg acgccattgt ccgtgataag tgccggttta atatggagac tccgttgtct
     4681 ccgaacgcgc gcaagaatac catcggggct tgcggggtgt caaacctctt tcccgagctg
     4741 attgggatgc cgaacccggc gatcaatact tccgcaggat tgcaggaggc aagttccaga
     4801 gaccccggcg caccgccccc caaagagcat tgaggaccgt aatcaggacc ggcgcagacc
     4861 gagcgaccag tttcaccacc agaccgggag ccgctggttg agacacacca cccagtacct
     4921 gaccgggatg ttcctcaaga atttccgcaa gagcggtttc aagtgacgtc cagacctgtg
     4981 tgtcgattcc gtctccgatc acaaagagcg acccgacata gtcccactcc tctgccaatc
     5041 catgaccgcc cgattgatcg gccggtgtga gggcataccg ttctccgacc gacgtccctg
     5101 aggccgtcgt aatccggata tcattctgga aactcgtgaa tttccatcgt tctccacgcg
     5161 cgatgcggcc tgatgcgatg gcatcccaaa gaatcagtga agcaccggcg gccagcttga
     5221 cgtcgatggt ttgacgaaag cgcgatccag caaagggaat cgtatgttcc ggtacccatt
     5281 cgaggatccc ttcaggccca acacgaattg ctatctcttg aacggagttc tctgacaagg
     5341 atcggtacac gcggttggcc gaaggcgtcg agatcagaac gtgagccttc ggtcccactg
     5401 cgagatcaat agagagatgg tcccctccga caagaccgcc ggagggattg ttcaacaacg
     5461 tataggcgct gccggtctca tcaagataga tgggcgggaa cagatgccag ggtgactggc
     5521 agctggttcg tccgaacacc gtgcggttgt tctgttggac gaattccaag gccaacctgc
     5581 cgactcgtcc gacggaggcg gtcggagcca ctgcagggat ttttcgtgga cgtggcacgc
     5641 gcgaccatgc ggcgaggtca gccccgatgg gcatgctgat ggatatgggg ttcaatccaa
     5701 ctcaccactc gatccagccc ttcgccggac atgaggttcg tgaagatgaa cggtcggtcg
     5761 ccccgcatgc ggcggctatc tcggtccatg acgccgagat cggcgccgac gtaaggggcg
     5821 agatcgattt tattgataac gaggaggtcc gagcgtgtga tgccggggcc tcccttgcga
     5881 ggaatcttat cgccttccgc cacgtcgatg acatagatcg tatgatccac gagttcggga
     5941 ctgaaggtcg cagccaagtt gtcgcccccg ctctccacaa acatgagttc aacatcggga
     6001 tggcgacgca gcagttcatc gagcgcttcc tgattgtggg acgcatcctc ccggatcgca
     6061 gtgtgcggac agccgccggt ttccacgccg agaatgcgat cctgcggcaa tgcgccgcgt
     6121 ctcgtcaaga attcggcatc ctcttttgta aaaatatcgt tggtcacgac ggccaggctg
     6181 tagcgatccc gtagtttgcg gcagagcgcc tcaaccagcg ccgtcttgcc ggatcctacc
     6241 ggcccgccga taccgatcac cggaatatgt ttcgcgcgcg ccgcggtgac acggccgctg
     6301 ccacagtgat gttcatcggc ccgatgcatc agccttccct cctccgacaa tcaggaacgg
     6361 aacaaccgcg attccagcct cgcatgtcgc atcgcataga tatcatgtac cggcgcccag
     6421 gagatcaaga cctcttgcgg cttggctgcc ttactgagat tggcaatcag cggcacccac
     6481 tgcctcaaca gatgttggcc ttcctgttgg ccgaccggca gaagtttcat ggctgccgac
     6541 acaaacccct gtgccgtctg atagagatat gcggccacgg tattctgtct cgaccagccg
     6601 caggcggcca gagtcaaccc catgcttaca ggaacatgac cgggcgtccg tccttcatcg
     6661 acggcagtgc gatagtcgcg aagaaggcgg caaccctgaa tctgttcggc agatactttc
     6721 acgatctgcc gccccatttg ccggctggcg agccgcgagt cgcggccgat cttgaacgta
     6781 tccaactcac aatcgacctt caacgcaggc tgaagcttgc cggtcgtcac tgcctgatga
     6841 gcaatcgcca cggcgaccgc ttcgcggcgg ctcaccccat gaagcaggag atcctcgacg
     6901 tagtgcgcga gatctttacc atcgcgcact gaaccacctt tcaccgccgc ctccaacccc
     6961 gacgagtagg catacccacc ggagggaaag aagctgtcga tgaagcggac cccatttaaa
     7021 aggctaagtg tgtccatcgt accttagtta gttggccagt cgcctcactc atattccctc
     7081 cttataggct gatcaaacag gtgtttgttc gcaccctccc aaccccggcg cgcctagacg
     7141 cgcgtttcca cggcaaggcc gcagggagcg aggaccccga ggcgtacgcg ggcagggtac
     7201 gttgaggggt acgagcgacc gagaacgaag tcgggcgcct gtttcatcag cctagaacag
     7261 aaaataccgc tgcgccatcg gaaggaccac cgccggctca caagtcagcc gcacaccgtc
     7321 agccgtcacc acgtaggtct ccggatcgac ctcaatcttg ggcaaataat cgttcagttt
     7381 gagatcccgc ttcttcacgc tgcggcaatt ctttgccgcc gccacccgtt tctgcaaacc
     7441 gagtttcttg ggaatctccc ggtccaaacc tacttgcgaa aggaacgtga tgcaggtgct
     7501 ggacagcgcc cggccgaagg caccgaacat tggccggctg atgattggtt ccggtgtcgg
     7561 gatcgacgca ttgggatcac ccatctgcgc ctgcgcgatg aaaccgctct tgaggaccat
     7621 ttcgg
//
LOCUS       DATDNW010000396         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_1014634, whole genome shotgun sequence.
ACCESSION   DATDNW010000396 DATDNW010000000
VERSION     DATDNW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014634"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1038)
                     /locus_tag="VI359_09420"
     CDS             complement(139..1038)
                     /locus_tag="VI359_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY6974512.1"
                     /translation="MKLENVNTIGIVGAGQMGRGIAQVCATFGYHVRLVDVAEQLITE
                     ALAKIRVSLERAIERGGLTDQQAKQALAFIHPVAQLDRLHDVQMVIEAIPEDLALKQE
                     LFAQLNRVCPPHAVLASNTSSISISKLGAASGRPDRVVGLHFMNPAPVMRLVEVVRGS
                     ETSESTWTLAMELAKRLGKIPVLSKDTPGFIVNRVLIPMINEAIFALDEGVASAADID
                     LAMTTGANHPVGPLALADRIGLDTVLAIAEVLYQDLGDPKFRPCSLLRRYVEAGWLGR
                     KTGHGFYRYETSGERHEATSHRT"
     gene            complement(1153..2295)
                     /locus_tag="VI359_09425"
     CDS             complement(1153..2295)
                     /locus_tag="VI359_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY6974513.1"
                     /translation="MATDIIMPQLGESIAEGIVVKWLIPVGGMIQKDQSLLEVETEKV
                     ALEIPSPATGILSEIIVKEGETVPVGTLLARIDSAPPSEVINRVGGVVVRPMEPALEG
                     QGHHSPAVRQLATEHGVDLSQVRGTGAGGRVTKKDVQDFVAQSGSPAKAANHTIEQSM
                     GEDVIALTQMRKTIAERMINSRHTSAHVSTFFEVDFTGIAKFRKDRNLTYLPFVVRAV
                     TRAVREIPILNSSWDERGIVVKKDIHIGIATALQDGLLVPVVRHADRKGLTQLAKEIA
                     DLADRARSKKLSPEEVQGGTFTITNHGGFGSLFSTPIIHQPQIAILGVGAIQKRAVVL
                     NDAIAIRPMGYLSLSFDHRVIDGATADQFMAKVKGYLEESHWEPIL"
     gene            complement(2344..>2965)
                     /locus_tag="VI359_09430"
     CDS             complement(2344..>2965)
                     /locus_tag="VI359_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY6974514.1"
                     /translation="GGVHGGPFHSECPEGWFFHSPGLKLVAPSTPYDAKGLLKAAIRD
                     PNPVIYFEHKYLYRRVKAVLPQEDYIVPIGKADIKRAGNDLSIITYGAMVHLALEAAQ
                     MLTNEGIDVEVVDLRTLIPLDKETVYGSVRKTSKVILLHEDNKTGGVGAEIAALLAEE
                     CFDSLDGPIIRIAPPDTPVPFSTPLEEFFLPKTADIISAARRLAAY"
BASE COUNT          615 a          867 c          800 g          683 t
ORIGIN      
        1 cttctcaggc gaccagtcct tcaagtcgtc gcgcgaatag atgcgattga tggcaagacc
       61 tgagagtgag gtaaatcgag atttgcgttc ttgcatcgac cccttcctct gcgagtgcag
      121 aagaccgcgt ctgagcgttc aagttctatg gctcgtcgct tcgtgtctct cgccgcttgt
      181 ctcataccta tagaatccat gccccgtctt acgccccagc catcctgctt cgacatagcg
      241 tcgcagtaag gaacagggac ggaatttcgg gtcgccgaga tcctgataca acacctcggc
      301 gatagccagc acggtgtcca atccaatccg gtcggcgaga gccagcgggc ccaccggatg
      361 attggcgccg gtcgtcatcg cgagatcgat atctgcggct gatgccaccc cttcgtccaa
      421 cgcaaagatg gcctcattga tcatggggat gagcacacgg tttacaatga accccggtgt
      481 gtctttggac agcactggga tcttcccgag ccgctttgcc aactccatag cgagagtcca
      541 ggtcgattcg gaggtctcag aacctcgcac gacttccacg agtctcatca cgggcgccgg
      601 attcataaaa tgcaggccca cgactcgatc gggccgtccg gaggcggctc caagtttgct
      661 gatcgaaatg gatgaagtat tgctggcgag gaccgcgtgc ggagggcaga ctcggttcag
      721 ctgtgcaaag agttcctgct taagcgccag gtcctccgga atcgcttcaa tgaccatctg
      781 cacgtcgtgc aaacggtcca gttgtgccac ggggtggatg aaagccagcg cttgcttcgc
      841 ttgttgatcg gtgagacctc cccgttcaat cgcgcgttca aggctgacgc gaatcttggc
      901 gagcgcttcg gttatgagct gttcagcgac atcaacgagc cgaacatgat aaccgaacgt
      961 tgcacaaact tgcgcaatgc ctctgcccat ctggccggcg ccgacgatgc cgattgtgtt
     1021 gacgttctcg agtttcaagg ccgtcgtctc gtcaggacat ttgttccacg attatcgcca
     1081 atgcctcttc gcctccggta cggagactgc caacccgcac ggcgctcacg atgactgcct
     1141 gttgtatccg actcatagaa ttggctccca atggctttct tcgagataac ccttgacctt
     1201 ggccatgaac tggtcggctg tcgccccatc aatgacccgg tggtcaaacg acaggctgag
     1261 gtagcccata ggacggatcg cgatggcgtc gttgaggaca acggctcgct tctggattgc
     1321 gccaaccccg aggatcgcaa tctgcggttg atgaatgatt ggtgtactga ataggctgcc
     1381 gaaccctcca tgattcgtaa tcgtgaaggt cccgccctga acctcctcgg ggctcaactt
     1441 tttagaccgg gcgcggtcag ccaagtccgc tatttctttg gcgagctggg taagtccttt
     1501 tcgatccgcg tgtcggacga ccggaacgag gagtccatcc tgcagtgccg tggcgatgcc
     1561 gatgtgaatg tctttcttga cgacgatgcc tcgttcatcc cacgacgagt tcaggatcgg
     1621 gatctcccga acggcgcgag tcacagcacg gacgacgaat gggagatagg tcaggttgcg
     1681 atctttccga aacttggcga tacctgtaaa gtctacttca aagaacgtgg aaacatgtgc
     1741 ggatgtgtgc cgactattaa tcatcctttc ggcgatcgtc ttccgcattt gcgtaagggc
     1801 aataacatct tcccccattg attgctcaat agtatgattt gccgctttag cgggactccc
     1861 actttgagca acgaagtcct ggacatcttt cttggtcacc cgccccccgg ccccagttcc
     1921 tcttacctgt gatagatcga ctccatgttc tgtcgcgagt tggcggacag cgggagagtg
     1981 atggccttgg ccctcgagcg ccggttccat gggacggacg acaaccccac cgacccggtt
     2041 gatgacttcg gagggaggag cactatcgat tcgtgccagg agcgtaccca cgggaaccgt
     2101 ctctccctcc ttgacgataa tctcactcaa tattcctgtc gccggtgagg gaatttccaa
     2161 tgccactttc tccgtttcca cttccaatag tgattgatcc ttctgaatca tccctccgac
     2221 cgggatgagc catttcacga caatgccttc cgcgatactt tctccgagtt gaggcatgat
     2281 gatgtcagtc gccaccgaag ccctccagct gtccttgtcg gctggcccct caccgacgat
     2341 caatcaatat gccgccagtc tccgcgctgc cgaaataatg tcagccgttt tcgggagaaa
     2401 aaactcttcc agaggcgtgc tgaaaggcac cggcgtgtca ggcggagcga tccgaataat
     2461 cggtccgtcc aaagagtcga agcattcttc agccaacagc gcggcgatct cggcgccgac
     2521 cccaccggtc ttgttatcct catgcaacag gatgaccttg ctggtcttgc gaaccgatcc
     2581 atagaccgtc tctttgtcga gcggaatcaa cgtgcggagg tcgacaactt cgacatcaat
     2641 cccttcgttc gtcaacatct gagctgcctc cagcgctaga tgcaccatcg ccccataggt
     2701 gatgatcgag aggtcatttc cggcgcgttt gatgtccgcc ttcccgatcg gaacaatgta
     2761 atcctcctgt ggcagcaccg ctttcacccg ccggtagaga tatttgtgct cgaagtagat
     2821 gaccggattg ggatcccgga tggctgcctt cagaaggcct ttggcatcgt atggagttga
     2881 gggggccaca agcttgagtc caggtgagtg gaagaaccag ccttctggac actctgaatg
     2941 aaagggacct ccatgcacgc cgccg
//
LOCUS       DATDNW010000397         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_405179, whole genome shotgun sequence.
ACCESSION   DATDNW010000397 DATDNW010000000
VERSION     DATDNW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405179"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /gene="yidC"
                     /locus_tag="VI359_09435"
     CDS             <1..1548
                     /gene="yidC"
                     /locus_tag="VI359_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY6974515.1"
                     /translation="RTQVRGTASEVDKKSSSEVARQPDEGKPSSSSSAVAPPTGEDTV
                     TIDTDLFRAELTNRGGVLKSWELKRYHTALPEQKPVQLVYSGGKFRGPLSITVSDADL
                     DKAIREGLYSLEKDFTTLNASHPVGHVTLRFQDPNRQLSVEKQLTFHHDSYVVDISLK
                     LDGVPQPFDVGLGTNFGVVEWGDGFIGLIGSASMVDGKVEKETPEAELERKGAVQWVA
                     LQDKYFLSVLMPRQATAAVIKKEGDKIVSAGVRTASLSSGAPVSLQLYAGPKEYDTLR
                     LLNVGLEDTIDFGWFIFGSWSVVKAVAKPIFYVLRFINDYTHNYGVTIILLTMALKLL
                     FVPLQYKSYKSMKQMQVIQPKVLAVQEKFKDDRDRLNKELIKLYRDHKVNPVGGCLPM
                     VLQMPVFVALFNILYMTIDLRQAPFMLWITDLSVQDPYYVLPLIMGATMVIQQKITPT
                     TMDPTQAKIMLILPVFMTFLFINFPAGLVLYWLTNNVLTILQQFGTDRYMFRNKPLIS
                     TGPAQSA"
     gene            1612..3030
                     /gene="mnmE"
                     /locus_tag="VI359_09440"
     CDS             1612..3030
                     /gene="mnmE"
                     /locus_tag="VI359_09440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY6974516.1"
                     /translation="MREDLSDTICAIATAAGEGGIGIVRLSGPDALSIADRVVRVRSG
                     KSLTSIASHTLHLADLVLPPTMGDNGDHGLDRKAVPFDEALIVYMKAPRSFTAEDVVE
                     VHSHGGPVVLGLLCEACVRAGARMAAPGEFTKRAFLNGRLDLTQAEAVLDTIRAKSAI
                     GLRMAQRHLHGELAREVDGARSILLGVLAHVEAGIDFVEEDITFVQREGLERAIKSAA
                     MVIGKLEATARDGRVLREGARVVIVGRPNVGKSSLMNGFLKQERAIVTPVPGTTRDVI
                     EESIDLDGVVVRFVDTAGIRETTDLVEQEGIRRTQSAQTDADLVLVVIDGHANLTDED
                     RQILKTVSNSNYLIAMNKGDLAQAVGRSDLPFGAEVFVTSAKTGLGLEELKVAIRARL
                     VSPGFEVNEDLVVTNVRHRTALRCAQDSLAHASESVRMGLAGELVAIDLRVAADALGE
                     ITGTITTDDILDKVFAEFCIGK"
     gene            3044..>3229
                     /locus_tag="VI359_09445"
     CDS             3044..>3229
                     /locus_tag="VI359_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY6974517.1"
                     /translation="MEQQFDVIVVGGGHAGCEAALAAARMGARTLLFTMDEDRVAQMS
                     CNPAIGGIAKGHLVKEID"
BASE COUNT          748 a          781 c          963 g          737 t
ORIGIN      
        1 cggactcaag tcagaggaac agcaagcgaa gtcgataaaa aaagcagctc tgaagttgcc
       61 cgtcaacctg acgaaggcaa gccttcgtcg agctcttctg cagtcgcacc accaaccggt
      121 gaagacacgg tgacgattga caccgacttg ttccgagccg agctgaccaa tcgaggtgga
      181 gtcctgaaga gttgggagtt gaagcggtac cataccgctc tccccgaaca aaagccggta
      241 caactggtct attcgggagg gaaattccgt gggcctttat cgatcacagt gtcggatgca
      301 gatctcgaca aggcaatacg agaggggctg tattctctcg aaaaagattt caccacgctc
      361 aatgcctctc atcccgtcgg acatgtgacg ctccggtttc aggatcctaa caggcaactc
      421 tctgtcgaga agcaactaac cttccaccac gacagctatg tcgtcgatat ctctctgaaa
      481 ctcgatggag tccctcaacc gtttgatgtg ggcttaggga caaacttcgg agttgtcgaa
      541 tggggagacg ggttcatcgg tctcatcgga tcggcgtcga tggtcgacgg caaggttgag
      601 aaggaaaccc ccgaggcaga gcttgaacgg aaaggagctg tccagtgggt ggcgctccag
      661 gacaaatatt ttctctccgt cttgatgccc cgccaagcca cggcagccgt gattaagaag
      721 gaaggcgaca agattgtctc ggccggtgtg cgaacggctt cgctatctag tggtgctccg
      781 gtatcactgc aactctatgc gggacccaaa gaatacgaca cgttacgatt gttgaacgtc
      841 gggctggaag acaccatcga tttcgggtgg tttatcttcg gaagctggag tgtcgtgaaa
      901 gcggtcgcaa agccgatttt ttacgttcta cggttcatta acgactatac gcataactat
      961 ggggtcacga tcattctact cacgatggcg ctgaaactcc tctttgtgcc gttgcagtat
     1021 aaaagttata agtcgatgaa gcagatgcag gtgatccagc caaaagtatt agcggtccag
     1081 gagaaattca aggatgaccg cgatcggctg aacaaggaac tgatcaaact ttatcgagac
     1141 cacaaggtga acccggtcgg aggctgcctt ccgatggtgc tgcagatgcc ggtgtttgtg
     1201 gccctcttca atatcctcta tatgacgatt gacttgcggc aagcgccgtt catgctctgg
     1261 atcaccgatc tctcggtcca ggatccctat tacgtgcttc cgctgatcat gggggcgacg
     1321 atggtcattc agcagaagat tacgcccacc acgatggatc ccacacaggc gaagatcatg
     1381 ttgatcctcc ccgtcttcat gacctttctc ttcatcaatt ttccagccgg actggtgttg
     1441 tactggctta ccaacaatgt gttgacgatc ttgcaacaat tcggcacaga ccgatatatg
     1501 tttaggaata agccgctgat ttcaacgggg cctgctcaga gcgcatagca gaagaatacc
     1561 aagagagtag ttgtccggga ttgaccggct gttgggggga caggagtccc tatgcgtgaa
     1621 gatctctccg ataccatctg tgcgattgct actgccgccg gagaaggcgg gattgggatc
     1681 gttcgtctca gtggccccga cgctctaagt atcgctgatc gagttgtccg agtacggtcc
     1741 ggaaagtcac tcaccagcat tgcgagccac accctgcacc tagctgatct ggtgttgcct
     1801 ccgacaatgg gcgacaacgg ggaccacggg cttgaccgaa aggcagttcc ctttgatgaa
     1861 gcattgattg tgtatatgaa ggccccgcgt tcgtttacgg ctgaagatgt ggtggaagtg
     1921 cattcccacg gaggccctgt ggtattgggt ctactctgtg aagcatgtgt gcgggcggga
     1981 gcccgcatgg cggcaccagg agagtttacg aaacgggcct ttctcaacgg acgtctggat
     2041 ctcactcagg ccgaggcggt gctggatacc attcgggcga agtccgctat cgggcttcgc
     2101 atggcccaac gtcatttgca cggggaattg gcacgggaag tcgacggggc gcggtctatc
     2161 ctcctcggag tattagctca tgtggaggcc ggtattgatt ttgtagaaga ggatattacg
     2221 ttcgtccagc gcgaggggtt ggagcgggcg atcaagagtg cagccatggt aattgggaaa
     2281 ctggaggcga cagcgcgaga tggacgtgtg cttcgggaag gcgctcgcgt cgtcattgtc
     2341 ggccggccga atgttggaaa atcaagtttg atgaatggat ttctcaagca agagcgtgcc
     2401 attgtcactc ctgttcctgg aaccacgcgc gatgtgattg aggagtctat cgatctcgat
     2461 ggagttgtgg ttcgctttgt agatacggcg gggatacgag agacgacaga tctcgttgaa
     2521 caagagggta tcagacggac gcagagtgct cagaccgatg cggatctggt gttagttgtc
     2581 atcgatgggc atgcgaatct aaccgatgag gataggcaga tcctaaagac ggtctccaac
     2641 tcaaactacc ttattgccat gaacaaggga gatctcgctc aggcggttgg acgctcggat
     2701 ttgcctttcg gcgcagaggt gtttgtgact tccgccaaaa ccggcctcgg tcttgaggaa
     2761 ttaaaggtcg ccatcagggc acggctcgtg tcgccgggtt ttgaagtgaa cgaggatctt
     2821 gtggtgacaa atgtacggca tcggacagcc ctgcgatgtg cgcaggactc gttggcccat
     2881 gcgtcagaat cagttcggat ggggctagct ggggaactcg ttgccataga cttacgggtc
     2941 gccgccgatg cgttggggga aattacgggc acgattacga cggacgacat tctcgataaa
     3001 gtctttgcgg aattctgtat cggaaagtag cgagtgaaga gcgatggaac aacagtttga
     3061 cgtcatagtc gtaggaggtg ggcatgcggg gtgcgaagcg gcattggctg ctgcgcggat
     3121 gggtgcccgc accttgctgt tcacgatgga cgaagaccgg gtcgcgcaaa tgtcttgtaa
     3181 tccggcgatt ggcggtattg cgaagggaca cctcgtaaaa gaaatcgat
//
LOCUS       DATDNW010000398         4070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_60611,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000398 DATDNW010000000
VERSION     DATDNW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60611"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VI359_09450"
     CDS             <1..1009
                     /locus_tag="VI359_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974518.1"
                     /translation="VGTPAMFEWIHAKPAMAASPLYWCPNQKADQQYSSKPRPGCVPW
                     VEKKEPAEQSEQDGVAPPKPAAREMKIENLQGEVSSFLQKYRRFLECCKTDFNELKQV
                     EALGDEVSELLEFAQANLSNHSMASRGIMLREMIGSVAKARADLDTLHAKLERIETLT
                     ERQNALDFEDAGRDSRTIQDTEEAIKKGIREPRLSTGPKTGADIGTAPAAGPNIGKTP
                     TTGTAIGAQGVVGQDAGASSKNSQEIGASGPTGFGIGATGRAGPSIGESNLNSESSSA
                     VGSSLQRSTVGSSLPDSSVGSSFGGSSVGSSLQDSTVGSSFGSSSVGSTLQDRRTAPQ
                     P"
     gene            complement(1041..2075)
                     /locus_tag="VI359_09455"
     CDS             complement(1041..2075)
                     /locus_tag="VI359_09455"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974519.1"
                     /translation="MSILSFTTRCLLPLLFFAVSLAGCNRSDPIVVIQLHPKNPDIIY
                     VATNDYIYKTRDGGRTWTNLSKGMSHSRVIAMTVDPAYPATVYAGTKGDAVYKSYDGG
                     QRWVSQRAGLDDATISSVVNQFVFDPADNTHLFAATTMGVFETKNGGDTWTKRMEGMK
                     EVLMVVTLAMDPTRRFVLYAGTSGGVYKSSDAAGHWEKVNNGLVSPDIIKSSRALGVT
                     AIQVDPFAPDTVYAATLAGLYKTTDGAKSWARIAQSLQDQMMIGMLVDRSRSGVLYLV
                     GRSGVHRSEDGGTTWAAMNSGFSTLNVRSIAQSQTDPALFYAGTNGSGLYRSRNWGET
                     WESVPTVLPN"
     gene            complement(2240..2737)
                     /locus_tag="VI359_09460"
     CDS             complement(2240..2737)
                     /locus_tag="VI359_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY6974520.1"
                     /translation="MADQFSFDVVSQVDMQEMKNVVDQATKEIKQRFDFKDSKTEITL
                     KEKEKELVVVSDDEYKLNAVNEIIKTKCVKRHVSLKALDYGKVEDALGATVRQVIKIQ
                     SGISSEKAKDISKAVRDSKLKVQAQIQGEQLRVLSKSKDELQATIAFLKSKDFGIDLQ
                     FVNFR"
     gene            complement(2747..>4070)
                     /locus_tag="VI359_09465"
     CDS             complement(2747..>4070)
                     /locus_tag="VI359_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY6974521.1"
                     /translation="GVVLLIAAVLFGRVVAQSSFAPWFEYERPTLVWLLAGYGFLASV
                     LPGWMLLVPRGYLSTFMKLGVVFLLGLGVILMAPTIEMPRVTTFASGGGPIIPGTLFP
                     FLFITIACGAVSGFHALVSSGTTPKMIEQESQATVGYGAMLLESFVGVMALIAASVLI
                     PGDYLGINTNLSSEALTAMGFPVSRIDELSRLVEVDVAGRPGGAVSLAVGMASIFAAL
                     PGMAGLMAYWYQFALVFEALFILTTIDTGTRVARYLIQEMGGRLYAPLKHINWWPGVL
                     ASSGLVVGSWAYLISTGSISTIWPMFGAANQLLGTLALCIGTTVLIKMWKSPYLWVTA
                     VPMVFVGLITLTGCYEMFGMFLTKAATLAASESFPLYLDAVLVAVVAILGLIVLGDSV
                     QQWYGYVIMKKPFTSSEVVVLAGGGSAGRLQTTVRADDAFHLPGGGCC"
BASE COUNT          911 a         1305 c         1057 g          797 t
ORIGIN      
        1 tgtggggacc ccggccatgt tcgagtggat tcacgccaaa cccgcgatgg ccgcctcccc
       61 gctctactgg tgccctaacc agaaagccga ccagcagtat tcatccaaac cccggccggg
      121 ctgcgtaccg tgggttgaga aaaaagaacc tgcagagcag tcggagcaag acggcgtcgc
      181 tccaccaaaa ccggccgcgc gcgaaatgaa aatcgaaaac ctccagggcg aagtctccag
      241 ctttttgcaa aaataccgtc gcttcctcga gtgttgcaag accgacttta acgaactcaa
      301 gcaggttgaa gcgttgggtg acgaggtctc cgagttgctg gagtttgcac aggcaaatct
      361 ctcgaaccat tcgatggcgt cgcgcggcat catgctgcgt gaaatgatcg gctccgtcgc
      421 aaaagcccgc gcggatctcg acacgctcca cgctaaactc gaacgcatcg aaacattaac
      481 cgaacgacaa aacgccctcg attttgaaga cgcgggacga gatagtcgca ccatacaaga
      541 cacggaagag gccatcaaga agggaatccg cgagccgcga ctttcgactg gtcccaagac
      601 cggcgccgat atcggcaccg ccccggcggc cgggccgaac attgggaaaa cccctaccac
      661 cggcactgcc atcggagctc aaggcgtcgt gggtcaagac gcaggcgcgt cttcgaagaa
      721 cagtcaggag atcggcgcat cgggaccaac cgggttcggc atcggggcca caggtcgggc
      781 agggccctcc atcggagaat caaacctcaa cagcgaatcg tcttccgcgg tcggatcgtc
      841 cctgcaacga tcaacagtcg ggtcaagcct gcctgattcg agcgtcggat caagcttcgg
      901 tggttccagt gtaggatcaa gccttcagga ctccaccgtc ggatcgtcat tcggttcatc
      961 ttcagtcggt tcgactctcc aagatcgtag aaccgcgcct cagccgtaac attcactcca
     1021 cccgctcttc agcatccttc ttagttcgga agcaccgtgg gaaccgattc ccacgtctct
     1081 ccccaattcc gactccgata caagccgctg ccgttcgtgc cggcatagaa caacgcggga
     1141 tcggtctggc tttgggcgat cgatcgcacg ttcagcgtcg aaaacccact attcatcgcc
     1201 gcccaggttg tcccaccatc ttcgcttcga tgcacgccac tccgtccgac caaatagagt
     1261 acaccggagc gtgaccgatc caccaacatc ccgatcatca tctggtcttg cagcgattgc
     1321 gcgatgcgtg cccaggattt tgcgccgtcc gtcgtcttgt agagtccagc gagcgtggcc
     1381 gcatacacgg tgtccggagc aaagggatcg acctggatcg ccgtcacgcc aagcgcccgc
     1441 gacgacttga tgatatccgg cgataccaac ccgttgttga ccttctccca atgccccgcg
     1501 gcatcggacg acttgtacac ccccccgctc gtgccggcgt acagaacgaa cctcctggtc
     1561 gggtccatgg ctagggtcac gaccatcagc acttccttca tgccttccat tcgcttcgtc
     1621 caagtgtctc cgccattctt ggtttcaaac acccccatcg ttgtcgctgc gaacaaatgc
     1681 gtattgtcgg ccggatcgaa gacgaactga ttgacgacgg acgaaatcgt cgcatcgtcc
     1741 agccccgctc gctgcgaaac ccaccgttgt ccgccatcgt agctcttgta gaccgcatcg
     1801 cccttggtac cggcatagac cgtggcagga taggccgggt cgaccgtcat cgcaatcacg
     1861 cgggaatggc tcatcccttt cgacagattg gtccaggtcc ggcctccgtc gcgcgtctta
     1921 tagatgtagt cattcgtcgc gacgtagatg atgtcgggat tcttcggatg gagctgaatg
     1981 acgacgatgg gatcactgcg gttacagccc gcgagggaaa cggcgaagaa gagtaacggg
     2041 agaagacatc tcgtcgtgaa agataagatg ctcaaacata aattcccacg atctagttct
     2101 gagaggtgac ggagattgtt ccacgtgcgc gcgtccaccg agcaccgccc ttgatttagg
     2161 aaaatgattg ctcttgtgtg ccgcggtgcg cgagcacaag aacaatcccc gtcgcctcac
     2221 cctgtttcca catcccgcgc tatctgaagt ttacaaactg caggtcgatt ccgaaatcct
     2281 tactcttgag aaacgcgatc gtcgcctgca actcgtcttt gcttttactc agcacgcgga
     2341 gttgctcgcc ctgaatttgc gcttggacct tgagtttcga atcacgaacg gctttgctga
     2401 tatctttcgc cttctccgac gaaatgccgc tctgaatttt gatcacctgc cggacggtgg
     2461 cgcctaaggc atcctcgacc ttgccgtagt ccagcgcctt gagcgacaca tgccgtttta
     2521 cgcacttggt cttgatgatt tcgttcaccg cgttcagctt gtactcgtcg tccgagacga
     2581 ccaccagttc cttttctttt tccttgagcg tgatctccgt tttggaatct ttgaagtcga
     2641 accgctgttt gatttctttg gtcgcctgat cgacgacgtt cttcatctcc tgcatatcaa
     2701 cttgcgagac tacgtcgaac gagaattgat cagccatgat aatctcctaa cagcatcccc
     2761 cgcctgggag gtggaatgca tcatcggccc tgaccgtcgt ctgcaaccgc cccgctgatc
     2821 cgccccccgc cagcaccacc acctcgctgc tcgtaaacgg cttcttcatg atcacgtagc
     2881 cataccattg ttgcacactg tcgcccagca cgatcaagcc aaggatcgcc accactgcca
     2941 ccaacaccgc atcgagatag agcgggaacg attcgctcgc cgccaacgtc gccgccttcg
     3001 tcaggaacat cccgaacatt tcataacacc cggtcagcgt aatcaagccc acgaagacca
     3061 tcggcaccgc cgtcacccag agatacggcg acttccacat cttgatcaag accgtcgtcc
     3121 cgatgcagag cgccagcgtc cccagcagct gattcgctgc gccgaacatc ggccagatcg
     3181 tcgagatgct gccggtgctg atcaaatagg cccacgatcc taccaccaga ccactgctcg
     3241 ccaacacacc cggccaccaa ttgatgtgtt tcagtggtgc gtagagtcgc ccacccattt
     3301 cctgaatgag ataccgcgcc acgcgcgtcc cggtgtcgat cgtcgtcagg ataaacagcg
     3361 cttcgaacac cagcgcgaat tgataccaat aggccatcag tcccgccatg ccgggcagcg
     3421 cagcgaagat ggacgccatg ccgaccgcaa gcgacaccgc tccgcctggt cgtcctgcta
     3481 catcgacttc caccaaacga gacagctcgt cgatgcgcga cacaggaaac cccatcgctg
     3541 tgagcgcttc cgacgagaga ttcgtattga tccccagata gtcgccggga atcagtaccg
     3601 aggccgcgat caacgccatc acgccgacga agctttccaa gagcattgct ccatagccga
     3661 ccgtggcttg ggattcctgc tcgatcatct tcggggtcgt accggacgac accagcgcat
     3721 ggaaacctga gacagccccg caggcaatcg tgatgaagag aaacgggaag agcgtgccgg
     3781 gaatgatcgg cccgccgccg gaggcgaacg tcgtcacgcg cggcatttca atcgtcggcg
     3841 ccatgaggat cacgccgagg ccaaggagaa agacgacgcc cagtttcatg aaggtggaga
     3901 gatagccgcg cggcaccagc aacatccagc ctggcagcac cgaagccagg aagccatagc
     3961 cggccagcaa ccacacgagc gtcggtcgtt catattcaaa ccacggcgcg aaggacgatt
     4021 gtgcgaccac acgaccaaac agcaccgctg cgatgagtag caccacgccg
//
LOCUS       DATDNW010000399         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_135983, whole genome shotgun sequence.
ACCESSION   DATDNW010000399 DATDNW010000000
VERSION     DATDNW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135983"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..836
                     /locus_tag="VI359_09470"
     CDS             219..836
                     /locus_tag="VI359_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY6974522.1"
                     /translation="MLQELTESHYGKNDVIFREGDPTEYFHIVKEGNVKCVKSTPDGK
                     ECTIKMLMPGDLFCCDAAAFEGACHPGTAQPMGNVSILRLSKKSYLDMLRRNPDAAIE
                     VIRYLGTRLHEAQEKTKILALDRADQRLASLLVDFAARNGMKDPRGIRLNLRLTRQDM
                     ANMVGITTETAIRIMSRFKRDHLVSGTANRLVIRDLPRLKTLATV"
     gene            1019..1999
                     /locus_tag="VI359_09475"
     CDS             1019..1999
                     /locus_tag="VI359_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY6974523.1"
                     /translation="MKQSPRIFCMLAVAASLLAAGAAVTSPTWAKTHEIAMTAVESDV
                     VIDGSGEKYAAWTFNGQMPGPVVRVTEGDTINFTLTNPETNKNPHAMDFHAAEIDFLK
                     NYRAINAGETISYTFVAKKPGIFFYHCGAPPMIQHIARGMFGAIIVDPKEVKALPKAD
                     REYVLIQSEYFKNPGDVQAMFDRKFDGMMFNGGIFKYHPFVTGGGKLDAKPGERVRIY
                     FVNAGPNEFSSFHPIGEIWDNVYESGNPANSLKGVQTYVVGPGSAATFDVVVESAGAY
                     PLVTHSLTGALRGAIAVLVVSPDAKPAPLMPMVPWKVATPQTEITPVPTP"
BASE COUNT          517 a          629 c          600 g          464 t
ORIGIN      
        1 agtaggctcg gaaggcattc gagtccacaa gctggccatc cgtgcgattc cgcatagcgg
       61 aaagccagaa gaactcgtgg atcactttgg catcggagcg cgatcgattg tcgaagcagc
      121 caaacaaatc ctcaagtaac cggtccggtt ctgacctccc gcacgagctc gaccggattc
      181 ctctcctgaa tatcctggct cgatcagatc gtcaacgcat gcttcaggaa ctcaccgaat
      241 cccattacgg aaagaacgac gtgattttcc gggaagggga ccccaccgaa tattttcaca
      301 tcgtgaaaga aggcaacgta aagtgcgtaa agtctacgcc ggacggaaaa gagtgcacca
      361 tcaaaatgtt gatgcccggc gatctctttt gctgcgatgc cgccgctttc gagggagcct
      421 gccaccccgg caccgctcaa ccgatgggaa atgtcagcat tctccggctg agcaagaaat
      481 cctacctcga catgctacga cgcaatccgg atgccgcaat cgaggtaatc agatatctgg
      541 gcacccggct gcatgaagca caggaaaaaa ccaaaattct ggcattagac cgcgccgacc
      601 agcgacttgc ctccctcctg gtcgacttcg ccgcacgcaa cggcatgaag gacccccgcg
      661 gtattcgatt gaacctccgg ctcactcgac aagacatggc caacatggtc ggcatcacca
      721 cggagaccgc cattcgaatc atgagtcgct tcaaacgtga tcacctcgtc tccggcaccg
      781 ccaatcgtct cgtcatccgc gacctccctc gcttaaagac tctggctacg gtctgacagc
      841 catctccctc gcgaagccag ggggcctcgt tcatgcgaaa acatgacatt ggtcatattg
      901 ttggccgtgg cttctgtgta tgctccaccc accaatcagc aaacggaaaa tcgccgagta
      961 cctccagatg ttcaggtatt cggttgtgaa tccaatatca cataggaggc gtatcggtat
     1021 gaagcaatca ccgagaatct tttgcatgct cgctgtcgcg gcaagcctgc tggctgcagg
     1081 cgcggctgtc acttcaccga catgggccaa gactcatgag atcgcgatga ccgcggtgga
     1141 atccgatgtt gtgatcgacg gcagcggcga gaagtatgcg gcctggacat ttaacggcca
     1201 gatgccaggc ccggtcgtgc gggtgacgga gggcgatacg atcaacttca cgcttacgaa
     1261 cccggagacc aacaaaaatc ctcatgcgat ggactttcat gcggctgaga tcgactttct
     1321 caagaactat agggcgatca atgccggcga aaccatcagc tatacgtttg tggccaagaa
     1381 gcccggcatc ttcttctatc actgcggggc gcctccgatg atccaacaca ttgcacgtgg
     1441 catgttcggc gcgatcatcg tcgatccaaa agaggtaaag gccttgccta aggcggatcg
     1501 agagtatgtc ttaattcagt ctgaatactt taagaatccc ggcgatgtgc aggcgatgtt
     1561 cgaccgaaaa ttcgatggga tgatgttcaa cggcggcatc ttcaaatacc acccgtttgt
     1621 gaccggtggc gggaagctgg atgccaagcc aggcgaacgg gtccggatct attttgtcaa
     1681 tgccgggcca aacgaatttt cttcgttcca tccgatcggc gagatttggg ataacgtcta
     1741 cgagagcggc aatccggcca atagcctgaa gggcgtgcag acgtatgtcg tggggccagg
     1801 cagcgcggcg accttcgatg tggtggtgga gtcggccggc gcatatccgt tggtgaccca
     1861 ttcattaacg ggcgcgctcc gtggcgcgat cgcagtcctc gtggtctcgc cggatgctaa
     1921 accagccccg ttgatgccga tggtgccgtg gaaggtggcg acaccacaaa ccgagattac
     1981 gcccgttcca actccataga aaggacagtg gaccgtttgg ctcctttgag ctggccagcc
     2041 gaggcagatg attcttttaa ggtcgtgatg aacagatatt tccttctctg cctcatactc
     2101 gtctggggag tcatttgcct tcccgctctc gcccatgcag cggatgagcg gattaccctg
     2161 atgctcgcag gaaccgcttg cccacagagt cacgcagtgt tggaacagta
//
LOCUS       DATDNW010000400         6963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447 k141_62300,
            whole genome shotgun sequence.
ACCESSION   DATDNW010000400 DATDNW010000000
VERSION     DATDNW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62300"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1617)
                     /locus_tag="VI359_09480"
     CDS             complement(187..1617)
                     /locus_tag="VI359_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY6974524.1"
                     /translation="MHGLALAALSMLLISCAMGPDYKRPDIPTSDSFRMAEAEKDLPS
                     LANVPWWELYRDEELQKLIRVALEENKDLKRAVATVDEFAARLLVAKTDFAPQMSVSA
                     NAPAFGNTKNVAFPGFPNPFNYYVRGDLAWELDIWGRIRRSNEAALGDLLAREDSRRA
                     LILQIVGGVAQAYFDLRQFDMQLEIATRTLRAWEDSVRISQARLRQGLINRLDVDQFE
                     AERENAAARIAELKRQMVQKENELSVLLGRNPNQISRGRSLTEQMMPPVVPAGLPSEL
                     LQRRPDVVQAEQQLAAATARIGQAKADRFPKLSITGILGVASPQLTRLVDHDTYFGVA
                     GPSLVGPLFNAQILGFQQEAAEAQARQAVAQYEQTILVAFREVEDALVGVSTAREQAT
                     AQEKQVKALQSALHLANLRYKGGLANYLDVLIAQRNLFDAELSLAATRRLHLVSVVQL
                     YKALGGGWSPEQAREDAEAKTVKASG"
     gene            complement(1619..4807)
                     /locus_tag="VI359_09485"
     CDS             complement(1619..4807)
                     /locus_tag="VI359_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY6974525.1"
                     /translation="MRSGFFIDRPIFAAVLSIVIVVVGLVSIQALPIAQFPNITPPVV
                     QIDADYPGASAEVVADSVARPIEVQLPGIDNLLYYDSTSTNDGHVTMRLSFEIGTDVD
                     IATVQTQNREKLAEPQLPPEVIRRGISIKKTSPDLLAVITLESSDVRHDALFISNYAI
                     LRILDNVKRLPGVGDATVFGAQSYSMRIILDPVRMAQLSLTPTDIIDVIREQNRDFPA
                     GTVGREPAPKGTELTIPVITLGRLTEVKEFEDMIVRALPNGSMIRLKDVARVELGAQS
                     YDLEGRFKGKPTTFLLTFLAPGYNALETVKRIRKEMDGLSKNFPPGMKYDVPYDTTKF
                     IEVSIEEVVKTLGEAMILVILVVYLFLQTWRATVIPAVAVPVSLIGTFAGMEALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVERHMETERCSAKEAAKRAMSEVSGPVIAIVLV
                     LCAVFVPVGFLGGITGQLYKQFAITIAISVIISGIVALTLSPALCAILFKPGHKTDPK
                     GFFRLFNRFFNWTQGRYTKTVGVVLHHSVVAVAVFVIVGVVAAGMLQIIPHSFLPDED
                     QGYFITVVQLPDGASKERTTAVVNKLEQYYLSIPAIHSTDALVGQNFVFGTRGSNTAT
                     MFTPLHLWDERKGPGEHVQALIGGAFQEFAKIPEALILAFNAPSIRGLGATGGFSVQI
                     QDPSGGDFKKFAAVAQEFTTKARQDPAIGAIGTNFRVTSPRLYAHINRERAKALGVPI
                     SEVFDTLQAFWGNFYINDFVKFGRVYRVQTESEPYYRSKPGDISKIYVRALNGQGPSM
                     IPLDTVVTTEYTSGPDPVTHFNGYNTALVLGAAAPGYSSGQALDALERAARETLVPKG
                     YDIDWSGISYQEHKVGGESAAAFGFGLLMVFLVLAAMYESWTVPFAVILAVPFGVFGA
                     LSAVLVRGMTNDIFFQIGLVTLIGLSAKNAILIVEFANKRYEHGMSMVDAALEAARLR
                     FRPIVMTSMAFILGVVPLVVATGAGASGRHSIGTGVLGGMLAATFLAIFFVPLFFVLV
                     RKLSPQRADPNAGKETGIAHARSDQSHEEGGH"
     gene            complement(4804..6006)
                     /locus_tag="VI359_09490"
     CDS             complement(4804..6006)
                     /locus_tag="VI359_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974526.1"
                     /translation="MTSSIRRPLKAGVGVLMCVGLAFVIGCKKEADSSPVKLAPQVEV
                     VMVEPQTVPDEPEFIGQAEASRIVEVRPQVTGIIKERFFSEGRDVKKNDRLYQIDPVP
                     FKAAEISARGRVAQAEARLVQAKQNLARVKPLLVEQAVSQKDVDDAVAEELSAKASLE
                     AAKGDLVKAKFDLDNTLIVAPIDGLIERTRYYEGRLVTAQTDLLTIIHQVDPMYVIVS
                     APESFLLKRRRDIIAQRIQHPGIYQLTGKITFVDGTTYSHEGVLDFADVGLRTETGSR
                     QARVVFPNPDRVLLPGQFVTVRFHGVSKPNVILVPQRAVQQGPTGPVVYVVAEGDKVE
                     VRDVKATTWQGDRWLIEDGLHSGERVMIDGFQRVMPGAQVNPVTMPNTAVASESPPPA
                     EGKAGQTP"
     gene            complement(6134..6451)
                     /locus_tag="VI359_09495"
     CDS             complement(6134..6451)
                     /locus_tag="VI359_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974527.1"
                     /translation="MLKITQHRDAASKSVSLLLEGRLAGPWVEELELYWRQTAGNPPT
                     RTVVDLTGVTFVDGDGKALLTRMWQQGAELRAAGCLTRCIVDEITGSGLGTSPCQDHH
                     KTG"
     gene            complement(6742..>6963)
                     /locus_tag="VI359_09500"
     CDS             complement(6742..>6963)
                     /locus_tag="VI359_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY6974528.1"
                     /translation="NILIDLDFLKPFEAHNIAEFTLEPGGNSTTVTWAMYGPNPYFAK
                     VMHLFFSMDHMVGTQFETGLANLKTIAEK"
BASE COUNT         1435 a         2098 c         2006 g         1424 t
ORIGIN      
        1 cgtaggctcg tcgttcaatg tcttcacggg taggttgcgg ggaaggcttg gctcgttttg
       61 ccgttgaggt ctggcgaccg gttttcgccg ctggtttctt ggattctcgc ttcggcatgg
      121 ctgcctcctt ggtgaaggtc atgaactgac cgtcggtggg ggtgtacgtc gcgccgtcaa
      181 agtatctcat ccgctggcct ttactgtctt agcctcggcg tcttcgcgag cctgttccgg
      241 cgaccagccc ccgcccaagg ccttgtagag ctgaaccacc gataccagat ggagacgacg
      301 ggtggcggca agcgacaatt ccgcgtcgaa gaggtttcgt tgcgcaatga ggacgtccaa
      361 atagtttgcg agtccgcctt tgtagcgcag attggcgagg tgcaacgccg actgtagcgc
      421 cttgacctgc ttctcctggg cggtggcctg ctcgcgggcg gtactgactc cgaccaacgc
      481 atcttcgact tccctgaacg caacgaggat ggtctgctcg tattgggcca cggcttgcct
      541 ggcttgcgcc tcagccgcct cctgttggaa gcccaaaatc tgcgcattga acaaggggcc
      601 gaccaagctc gggcctgcca caccgaaata agtgtcgtgg tccaccagtc tggtcaactg
      661 tggactggcg acacccagaa taccggtgat gctgagcttg ggaaaccggt ctgccttggc
      721 ctgcccgatc ctcgcggtcg cagcggccaa ttgttgttca gcctgcacga cgtccggacg
      781 gcgctggagt agttcggaag ggaggccagc cggtaccact gggggcatca tctgttcggt
      841 gagcgaccgc cctcgagaga tttggttggg atttctcccc agcagcacgc tgagctcgtt
      901 ctctttctga accatctgcc gcttcagctc cgcgatgcga gctgcggcat tctcccgctc
      961 ggcctcgaac tggtcgacat cgagccgatt gatgagccct tgcctaagcc gcgcctggct
     1021 gattctcacg gaatcttccc aggcccgcag cgttcgcgtg gcgatctcca gctgcatgtc
     1081 gaactgccgc agatcgaaat aggcctgcgc caccccgccc acgatttgca ggatgagggc
     1141 gcgtcggcta tcttctcgag ccaagaggtc gccgagcgcc gcctcgttcg agcgccggat
     1201 ccgtccccag atatcaagtt cccaggcgag atcgccccgc acataatagt tgaacgggtt
     1261 ggggaacccg gggaaggcca cattcttcgt gttgccgaag gccggcgcgt tggcagacac
     1321 gctcatctgg ggggcaaaat cggttttagc gacgagcagc cgcgcggcga actcgtcaac
     1381 ggttgcgact gcccgtttga gatccttatt ctcctccagc gcgacgcgga tcaacttttg
     1441 aagctcttcg tctcgataga gttcccacca cggcacattg gccagggacg gtaagtcctt
     1501 ctccgcctcg gccatgcgaa acgagtcaga cgttggaata tcggggcgtt tgtagtccgg
     1561 ccccatggca caggagatca acagcatcga gagcgctgcc agagcgagac catgcatatc
     1621 aatgccctcc ttcttcatgt gactgatctg atctcgcgtg cgcgatcccg gtttcctttc
     1681 ctgcattggg atcagcgcgc tgcgggctca gcttccggac gagaacaaag aataggggaa
     1741 caaagaagat agccaagaag gttgctgcga gcatgccgcc gagcacgcct gtcccgatgg
     1801 aatgccgtcc cgatgcgcca gctcccgtgg cgacaaccag cgggacgact ccgagaataa
     1861 aggccatcga ggtcatcacg atcgggcgga agcggagccg ggccgcttcg agcgctgcat
     1921 caaccatcga catcccgtgt tcataccgct tgttggcaaa ctccacgatc aggattgcat
     1981 tcttcgccga caggccgatc agggtgacca ggccgatctg aaagaaaata tcgttcgtca
     2041 tgccgcgcac caaaacagcc gacaaggccc caaagacgcc gaacggaacg gcgagaatca
     2101 ccgcaaacgg cacggtccaa ctctcgtaca tcgcagccaa cacgagaaac accatgagga
     2161 gcccgaatcc gaacgccgcg gcagactcac ctccgacttt gtgttcctgg taggaaatgc
     2221 cgctccagtc aatgtcgtac cctttcggca ccagtgtttc cctcgccgct cgctcaagcg
     2281 cgtcgagagc ttgtcctgag ctgtagcctg gtgcggccgc gccgagcacg agggccgtgt
     2341 tgtagccgtt aaagtgcgtg accggatcgg gcccgctcgt gtattcggtc gtcacgaccg
     2401 tgtcgagcgg aatcatggaa gggccctggc cgttcaaggc gcggacgtat atttttgaaa
     2461 tatcccccgg cttggatcga tagtatggct cggactctgt ttgcacccga tagacccgtc
     2521 cgaatttgac gaaatcgttg atgtagaaat tgccccagaa tgcctgcaag gtatcgaaaa
     2581 cttccgagat cggcacacct aacgccttgg cacgctcgcg attgatgtgg gcgtacagcc
     2641 gaggcgaggt cacgcggaag ttcgtcccga tcgcgccgat cgccggatcc tgccgagcct
     2701 tggtcgtaaa ctcctgggcg acggccgcaa atttcttgaa atcaccgccg ctcggatcct
     2761 gaatttgcac cgaaaagcct ccggtcgccc ccaagccccg gatggacggt gcgttgaacg
     2821 ccaggatcaa ggcctccgga attttagcga actcctgaaa ggccccaccg atcaaggctt
     2881 gcacgtgctc gcccggtccc ttgcgttcat cccagagatg cagtggggta aacatggtcg
     2941 ccgtattcga tccacgcgtt ccaaacacaa agttttgccc caccaaggca tccgtactgt
     3001 ggatcgcggg aatggagaga tagtactgct cgagcttgtt cacgaccgcg gtcgtccgtt
     3061 ccttcgacgc tccgtccggc agttgaacca ccgtgatgaa gtatccttga tcttcatcag
     3121 gcagaaagct gtgcgggatg atctgtagca taccggccgc gaccacccca acgatgacaa
     3181 agaccgcgac cgccaccacg gaatgatgga gcaccactcc cacagttttc gtatagcgac
     3241 cctgcgtcca gttaaagaac cgattgaaga ggcggaagaa gcccttcggg tcggtcttgt
     3301 gcccgggctt gaacaggatc gcgcacagcg ccggactcag cgtgagcgcc acgattccgg
     3361 aaatgatgac cgaaatcgcg atcgtgatgg cgaattgctt gtagagctgc ccggtaatgc
     3421 cgcccaggaa tcccaccggc acgaacacgg cgcagagcac aaggacgatc gcaatgaccg
     3481 gaccggagac ttccgacatg gcccgcttgg cggcttcttt ggcggagcag cgttccgtct
     3541 ccatgtgccg ctcgacattc tccacgacga caatcgcatc gtcgaccacg atgccaatgg
     3601 cgaggaccat cccgaacaga gtcaacgtgt tgatcgaaaa ccccaaggct tccatgccgg
     3661 cgaaggtgcc gatcagcgag accggaaccg cgacagccgg aatcacggtg gcgcgccagg
     3721 tctgcagaaa caggtacacg accaagatga ccaggatcat cgcctcaccc agtgtcttca
     3781 cgacctcctc gatcgacacc tcgatgaact tggtggtgtc gtatgggacg tcgtacttca
     3841 tgcccggggg gaaatttttc gagagtccat ccatctcctt tcggatgcgc ttgaccgtct
     3901 ccagcgcgtt atagccggga gccagaaagg tcagcaggaa cgtggtgggc ttccccttga
     3961 accgtccctc gagatcgtag gactgcgcgc ctagctcgac tcgggccacg tccttgagtc
     4021 ggatcatgga gccgttcggc aaggcacgga cgatcatgtc ctcgaattct ttgacttcgg
     4081 taagccggcc aagcgtgatc acaggaatgg tgagttcggt gcccttcgga gcaggttctc
     4141 gccccacggt gccggccggg aagtcgcgat tttgttcgcg gatgacgtcg atgatatcgg
     4201 tgggcgtgag gctgagctgc gccatccgca cgggatccag aatgatccgc atactgtagc
     4261 tctgggcgcc gaatacggtg gcatccccca caccgggcag tcgtttgaca ttgtcgagga
     4321 tgcgaagaat ggcgtaattg gaaataaaca gggcgtcgtg tctcacatcg ctcgattcca
     4381 gagtgatgac ggccaggagg tccggcgacg ttttcttgat cgagataccc cgccggatga
     4441 cttcgggagg gagctgcggt tctgccagct tttcccggtt ctgtgtctgc accgtggcaa
     4501 tatcgacgtc ggtcccgatc tcaaacgaca acctcatggt gacatggcca tcgttggtgc
     4561 tggtcgaatc gtaatagagg agattgtcaa tccccgggag ctgcacctcg atcggacggg
     4621 ccaccgaatc cgcaacaact tcggcgctcg cgcctggata gtcggcgtcg atctgaacga
     4681 caggaggggt gatgttggga aactgagcga tcggcaacgc ctgaatcgaa accagaccaa
     4741 ccaccacgat gacgatggac agcaccgcgg cgaaaatcgg gcggtcgatg aaaaatccgg
     4801 atctcatgga gtctgtccgg ccttgccttc agcgggcgga ggagattccg acgccaccgc
     4861 cgtgttcggc atcgtcacgg ggttgacctg cgcgcccggc atcactcgct gaaatccgtc
     4921 gatcatgacg cgttcgcccg agtggagtcc gtcctcgatg agccagcgat ctccttgcca
     4981 agtggtcgcc ttcacgtccc gaacctcgac tttatcaccc tcggcaacca cgtagacgac
     5041 cggcccagtc ggtccttgct gcaccgctcg ctgcgggacc aagatgacat tgggctttga
     5101 cacgccgtga aagcgaacgg tcacgaattg cccgggcagc aagacgcggt cgggattcgg
     5161 gaagaccacg cgcgcctgcc gcgatcccgt ctcggtccgg agccccacat cagcgaaatc
     5221 gaggacacct tcgtgcgaat aggtggtgcc atccacaaag gtaatcttgc ccgtcaattg
     5281 atagatgccg ggatgctgaa tccgttgcgc gatgatgtcc cgtctccgct tcaagagaaa
     5341 gctttccggc gcgctcacga tcacatacat cggatcgact tgatggataa tggtcaacag
     5401 atcagtctgc gccgtcacca accgtccctc atagtagcgg gtccgttcga tcaagccgtc
     5461 gatgggcgcc acgataaggg tattgtcgag gtcgaacttc gccttgacca gatctccctt
     5521 tgccgcctcc agcgaggcct tcgcggagag ctcctctgcg actgcatcat cgacatcctt
     5581 ctggctcacc gcctgctcga ccagcaacgg tttcacgcga gcgagatttt gtttggcctg
     5641 gaccagccgg gcttcggctt gagccaccct ccccctggca ctgatctcag ccgccttgaa
     5701 tgggaccgga tcgatctgat agagacggtc gttcttcttg acgtcacgcc cttcggagaa
     5761 gaaacgctct ttgataatcc cggtcacctg cggacgaact tcaacgatcc gggaagcctc
     5821 cgcctgtccg atgaattcag gctcgtctgg cacagtttgc ggctcgacca tcacgacttc
     5881 gacctgcggc gccagcttca ccggggaaga atccgcctcc ttcttacaac caatgacaaa
     5941 ggccaaccca acacacatta ggacaccgac acctgctttg agtggtcgtc tgatggaaga
     6001 agtcatgatc atgatctccg tggtctttta tctccgctgg actctgggcc cgcgcggcaa
     6061 gaaaatcaac cgagtgatct ggtacagatc aggcaagctg atgaccggct ggtccattct
     6121 acgcccgcct ctcttaccca gtcttgtggt gatcctgaca tggtgacgtg ccgaggccag
     6181 agcccgtgat ttcgtcaacg atgcatctgg tcaaacagcc ggctgcgcga agttctgcac
     6241 cctgttgcca cattcttgtc agaagcgcct ttccgtcacc atccacgaac gtcacgccgg
     6301 tcaagtcgac cacggtgcgc gttggaggat tccctgccgt ctgacgccag tacaactcga
     6361 gctcctccac ccacggaccg gcgaggcggc cttcgagcag gagcgaaacg gatttcgacg
     6421 ccgcgtctcg atgttgtgta attttgagca tttcttctat ccccttgctc cgctgtaggg
     6481 gcaagggttg ttccagcggc gggagatggc gacaattctc caacctcttg aaagaacaat
     6541 tggaatttta agaggtcgcg acccgactaa gcaactgacg acgagatgct gccgatatcc
     6601 cggcacacgc cgaagagtcg gcaggaaaag ggtagagggt gggctcttat ggcatcggca
     6661 tggctaggct ttcaccaggg tcttgaacca cccgagacca tgcgcgtgac gtcgacgggc
     6721 atcaccgatc agcagtctag tttatttctc agcgatggtc ttcaggtttg caagcccggt
     6781 ctcaaactga gtgccaacca tgtgatccat actgaagaag agatgcatca ccttcgcgaa
     6841 ataggggttg ggaccataca tcgcccatgt gacggtcgtg gaattgccgc cgggttccaa
     6901 cgtgaactcc gcgatgttgt gcgcctcgaa gggcttcagg aaatcgagat cgatcagaat
     6961 att
//
LOCUS       DATDNW010000401         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_339471, whole genome shotgun sequence.
ACCESSION   DATDNW010000401 DATDNW010000000
VERSION     DATDNW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339471"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /gene="nuoG"
                     /locus_tag="VI359_09505"
     CDS             complement(<1..2071)
                     /gene="nuoG"
                     /locus_tag="VI359_09505"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY6974529.1"
                     /translation="MAEKTTETVRMTIDGLPVTVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEIEKMPKLQTSCSTPVAEGMAVRTATTVVNDAHKSVLEFILANHP
                     LDCPVCDQGGRCDLQDFSHEYTATASRFVETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEVMDVKALAPAGRGTMTEIKHFGPHALDCEFCGGCVQICPVGAITSRLSMYEYR
                     PWMLKRADTVCAFCGDGCQITVQTKDTELIEVMSAHGAGRNNGDLCARGFFGFHATSH
                     PERLTHPLIRRNGVLVQTTWEEALEYVAEHATKLKLAHGAQAFGGLITGRCTNEELYL
                     FQKFMRLTIGTNNLDSSARYGHLNGIQALRRIQGTHRWTVSFEDLVEADVLLLVGTNI
                     TETNPITGLKVKEAVKKGRATLLTIDSMQPVIGTISNIATLAHQHLCVGPTQLGNAVL
                     GLLKAVVEGNLIDPALSQQARGYTSAIIQALQQIPWEALTGATGLSQDAFVAMARTFS
                     PGRRVVILAGQGVLQGPGGFGICLNLMDCLLLTGKLTAPGCGLAPLAKENNDQGAVEM
                     GAVAEFLPGALAAADATARDRIAAVWREELPSTPGATLTEMVERVRTGALKALFVVGE
                     NPVGTLPAHSAVAQSLAKLDLLVCQELFLTETARLAHVVFPAASYMEKAGTFTNTEGH
                     VQAVRPAI"
     gene            complement(2074..>3354)
                     /gene="nuoF"
                     /locus_tag="VI359_09510"
     CDS             complement(2074..>3354)
                     /gene="nuoF"
                     /locus_tag="VI359_09510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY6974530.1"
                     /translation="KNMLQPGYTGSLAEYERTGGYQAVRKILGKMAPMDVTQLVMKSG
                     LRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERDPHQMLEGIV
                     LACYAIGSEAAYIYIRGEFVLGAAILERAIAEARTAGYIGKNILGSGTTIDVWVHRGA
                     GAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLANLPHIINRGP
                     EWFASIGSPPKSTGTRIFCVSGHVKRPGNYEVPMGITFRELIYEHAGGMRTNKPLKAF
                     IPGGASAPFLTPDHLDVKMDFESVAAAGSMLGSGGVTIMEEGTSMVWAALRLMEFFYH
                     ESCGKCTPCREGSSWLVQTMRRIVAKQGRIQDLETLTDLCKNIAGRTVCAFGDAEVAP
                     IMSTLKHWRSEYMSLIHEAEAANLLRPERLAARH"
BASE COUNT          638 a         1010 c          994 g          712 t
ORIGIN      
        1 cgatcgcggg acgtaccgcc tgcacatgcc cttccgtatt ggtaaaggtg ccggcctttt
       61 ccatgtacga ggctgccgga aagaccacgt gcgcgagccg agcggtctct gtaagaaaca
      121 gttcctggca gaccaagagg tccagtttcg cgagggattg tgcgacggcg ctatgggcgg
      181 gcagcgttcc gacgggattc tctcccacga cgaagagtgc cttgagagca ccggtccgca
      241 cgcgctcgac catctcggtc aaagtcgcgc ctggtgtgga gggaagttcc tcccgccaga
      301 ccgcggcaat gcgatctcga gcggtggcat ccgctgcagc cagagcgccc ggtagaaatt
      361 ccgccaccgc gcccatctcg acggctcctt gatcattgtt ctctttggca agcggcgcta
      421 atccgcagcc gggcgcagtc aattttcccg tgaggagcaa acagtccata aggttgagac
      481 agataccgaa gccgccgggg ccttgaagga ccccctgacc agcaaggatc acgacacgcc
      541 gtccgggcga gaacgttctt gccatagcaa cgaaggcgtc ctgcgagagt ccggtggctc
      601 ccgtcagggc ttcccatggg atctgctgta aggcctgaat gatcgcgctt gtgtatcccc
      661 gcgcctgctg actgagagcc ggatcgatca gattcccttc gaccaccgct ttgaggagcc
      721 ccagcacggc gttcccgagc tgggtcggtc cgacacagag gtgctgatgg gccagggtgg
      781 cgatattact aatggtgccg atgaccggtt gcatcgagtc gatggtcagc agcgtggcgc
      841 gacctttctt cacggcctct ttcactttga ggccggtgat gggattggtt tccgtaatgt
      901 tggtgccgac caacaggagc acgtccgcct cgacgaggtc ttcgaagctc accgtccacc
      961 gatgcgtgcc ctgtatgcgg cgcagggctt ggatgccgtt caggtgcccg taccgggcgc
     1021 tgctgtcgag gttgttcgtg ccgatcgtga gccgcatgaa tttttggaat agatagagtt
     1081 cctcattcgt gcatcgtccc gtgatcaatc ccccgaatgc ctgtgcgcca tgggccaact
     1141 tgagtttggt ggcatgttcc gcgacatact ccagcgcctc ctcccaggtc gtctgaacca
     1201 agacgccgtt tcgtcggatc aagggatggg tcagacgctc cggatggctt gtggcatgaa
     1261 atccaaagaa gccgcgcgca cagagatcgc cgttgttccg tccggctccg tgagcggaca
     1321 tgacttcgat cagctcggtg tcctttgtct ggaccgtgat ctggcagccg tccccgcaga
     1381 aggcacagac cgtatcggct cgcttgagca tccagggccg atattcgtac atggacagtc
     1441 gactcgtgat ggcgccgacc gggcatatct gcacgcatcc gccgcagaac tcgcaatcga
     1501 gcgcatgcgg gccgaaatgt ttgatctccg tcatcgtgcc acgaccagcc ggagccaagg
     1561 ccttcacgtc catgacctcg tcgcaatagc ggacgcaccg aagacattgc acacagcggt
     1621 tcatctgggt ttcgatgagg gggctgaagt attccttctg gaagatccgt ttcgtctcga
     1681 cgaaccggct ggcggttgcc gtgtactcgt gagaaaaatc ctggagatcg cagcggcctc
     1741 cttggtcgca cacggggcaa tccagcgggt ggttggccaa aatgaattcg aggaccgact
     1801 tgtgcgcgtc gttgacgacg gtggttgccg tccgcacggc cattccctcg gcgaccggcg
     1861 tgctgcagga ggtctggagc ttcggcatct tttcgatttc caccaaacac atccggcaat
     1921 tggcgtcagg cttcagcttc gggtggtagc agaaatgcgg aatcatgacc ccaacgcggc
     1981 gtgcggcctc gatcacgagc gtgcctttcg gtactgtgac cggcaggccg tcgatcgtca
     2041 tgcgaacggt ttctgtcgtc ttttcagcca tgattagtgc cgtgccgcaa gccgctcagg
     2101 gcgcaggaga ttcgccgcct cagcttcgtg aatgagtgac atatactcgg atcgccaatg
     2161 cttcaacgtg ctcatgatcg gggccacttc ggcgtcgccg aaggcacaga ccgtgcggcc
     2221 cgcgatgttc ttgcacagat cggttagggt ttcaaggtcc tgaatccggc cctgtttggc
     2281 gacgatccgg cgcatcgttt gcaccagcca cgagctgcct tcgcggcaag gcgtgcattt
     2341 gccgcaggac tcatgataga aaaattccat gagacggagg gctgcccaga ccatgctggt
     2401 gccttcttcc ataatggtca cgcctccgga tccaagcatc gaccctgcag ccgcgactga
     2461 ttcgaagtcc atctttacgt cgagatgatc cggcgtcaga aaaggagctg aggcgcctcc
     2521 tggaatgaac gctttcaagg gcttgttcgt ccgcatgcca ccggcatgct cgtaaatcaa
     2581 ttcacggaac gtgatgccca tcgggacttc gtaatttccc ggccgcttca catggccgct
     2641 gacgcagaag attctggtcc ccgtactttt gggtggcgat ccgatggagg cgaaccactc
     2701 tgggccccga ttgataatgt gcgggagatt cgccagggtc tcgacgttat tgacgaccgt
     2761 cggtttgttg tagagcccat gcgttgcggg gaacggcggc ttcacacgtg gcagcccccg
     2821 cttcccctcg agcgattcca agagggcggt ttcctctccg cagatgtatg cacccgcgcc
     2881 tcgatgcacc cacacgtcga tagtcgttcc gctgccgagg atgttcttac cgatatatcc
     2941 ggccgtccgt gcttcggcga tcgctcgctc gagaatcgcg gcgcccaaca caaactcacc
     3001 ccgaatataa atgtaagcag cctccgatcc gatcgcataa caagccagca cgatcccttc
     3061 gagcatctgg tggggatcgc gctcaatgag ttgacgatcc ttgaatgttc ccggttcgct
     3121 ctcatcggcg ttacagcaga ggtagcgtgg gccctggtaa tccttgggga gaaaacccca
     3181 tttcacgccg gtcggaaagc cggcgccgcc gcgaccgcgc aaaccggatt tcatcaccaa
     3241 ttgcgtgacg tccattggag ccattttgcc gagaatcttc cgaaccgctt gatagccgcc
     3301 ggttcgctca tactcggcca gcgagccggt gtaaccgggc tgcagcatgt tctt
//
LOCUS       DATDNW010000402         6827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_339723, whole genome shotgun sequence.
ACCESSION   DATDNW010000402 DATDNW010000000
VERSION     DATDNW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339723"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..879
                     /locus_tag="VI359_09515"
     CDS             55..879
                     /locus_tag="VI359_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY6974531.1"
                     /translation="MPLRNGLSEELNRQGNEHFGRGHYTDAYACYAKALECDRITGDR
                     RALAATLGNMGNICAVSGRREAAQNYYQEVLELQKILGDEKGIGTTLGNLGNLRADAG
                     EWDRARAYYLEALDLMTKTHDEAGKAVLFSDLGLVARETKQFDEALRYYEQSLVLMRR
                     LGNQGGVADAWRMMGRTFLMQQRHDDAIACCQTSQSIAERTHDELRAGGARYVLAQCH
                     EEMGRLQEAADLLEQVVAMDRKYQLPKLEENTKRLESLRARLAGEASAASQRRSKR"
     gene            876..1313
                     /locus_tag="VI359_09520"
     CDS             876..1313
                     /locus_tag="VI359_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974532.1"
                     /translation="MSCPGSTDWADVRAALTKSVPQFYELEPGGALALDLGGDGWLLE
                     VRPDGHVLCQYGVSMEDVKSLMSDGTPEDLGTDEIAKQAKYFIQPAVAKFRPLLLQSG
                     FVEETEMNEEYVAVTFHRSVDFQNLSTVQGLVQWCRRSIGAGS"
     gene            1310..2386
                     /locus_tag="VI359_09525"
     CDS             1310..2386
                     /locus_tag="VI359_09525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974533.1"
                     /translation="MTGPRKPISNFEELRDALAAYRLPRVILAGLELELFTRVGDKTW
                     TIPELAKCLQVSERGLAILCRNLAMAGLLIKKGATYRNSRLAATALNANHPAYRGSYL
                     DLLKNHWADWGRMLESVRTGLPLDHDVPEEPDYRRRFTWAMHHRTLDVAPSIAAQVSL
                     RGAKTLLDLGGGPGTYAMAFLAKNPNLRATVCDRPDALDVAREIAATHKAGRRMSYLP
                     LDLMKDEIPGTFDVVWYSNVLHIYSPEENQGVFKRVSAALNPGGRLIIQDAFLHDREG
                     LFPEEASLFAVSMLLFTETGNTYSVMETSDWLKQAGFTAVRLLRIKKGLEDWEGGILE
                     GRVSAKDARRNGRPTRSRKNSRTR"
     gene            2744..3184
                     /locus_tag="VI359_09530"
     CDS             2744..3184
                     /locus_tag="VI359_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEY6974534.1"
                     /translation="MAMTEDRRSTSAGCLLVYDGQCRLCVTAKEGLERLGTQDEAKAV
                     RLIPYQSEEAKRALGATYRPGRPDVAFLVQPNGVIQQGLDAFLPLLPGLRGGRFLATI
                     LSVPVIKPFAYLIYRLVARYRYRLFGEVPYTTVDDPAQKKSSDR"
     gene            3487..4320
                     /locus_tag="VI359_09535"
     CDS             3487..4320
                     /locus_tag="VI359_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974535.1"
                     /translation="MSGLCIPVSAWSGDLSGVHQVEFPTSARPVVAAGFGFQNADSST
                     ITVRTYDALTGEILSDESYDLNVKEEGTSSSRQPRERIFAGGVGLGADGLSDFTLRVY
                     DAATGKFLWEGRLNLSVNDGGAGSTYRIVAHLQPQTSMRTIRQVQRANGQPSFLLRAT
                     DPVTGQLIWADQFSAGRGIVPRIERISEGAAGQPEGSATWFKNFDFRIRMFDDQNRQM
                     IWEDAIVPADETGDLSLGRDSEADTLPAWANGRNSMRKDAIRFEGLLRSAEGIMRSST
                     L"
     gene            complement(4317..5744)
                     /locus_tag="VI359_09540"
     CDS             complement(4317..5744)
                     /locus_tag="VI359_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974536.1"
                     /translation="MYRRNISQTLFYVACVLGLFIVYHAWLKPTYLPPAPAPQSEHLV
                     PPRSEVIPEPPPEVAVPEPKQTRVIDQTAVPDTKGRELLEAIREEIEKGNVKEAEMKL
                     GDLPSWLVSNEKTRPYVAVLWNNLGIEQEKSGGTASSVKTFRKAASLDTKNPAIQLNL
                     AHAYWELRDPTMTPEYLEKLIALAPNEPFPHVALADMLQEQDRLEEAAHHLDLAADRA
                     GKDPGIQSYLRAVTAKVRQTGKLEQRLNSRESTHFTVKYDGDADQNTWTTVLEILEDA
                     YREIGQKLNHFPSKPIVVVLHATESFQSTTGSPAWADGLYDPVLGRIQVPTQGALTDQ
                     AWLRRVLRHEFVHALLQDQLGLNATAIPTWLNEGLAMTLSADRWEDLDQLRHQNFTVI
                     PLPTLEGPWGRLPQDAASVAYLEAYSAVQYMIDRYGMHRVHELLSHLKARQALPVAMQ
                     SQLSLSYEQFQARWMEHLHERTKKS"
     gene            5858..>6827
                     /locus_tag="VI359_09545"
     CDS             5858..>6827
                     /locus_tag="VI359_09545"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY6974537.1"
                     /translation="MIRSHLDMWRSSEPKPTDPFQQWLDRVSRPIDFASRDAFAHLPT
                     VKNLNRFVSSQVVQALSDRVYPRAIETALLRLRELFVEDRQRLSTIEQQRRLREAATI
                     LSTLRAAAQDPAHAWNEPQGVATSSSTRHGPVSSELWKVSIRFAKGVGPQRTALLNRM
                     GIATVEDALWTVPWRYEDRSVITPIGSLAVGAQASICGTIVHSKAKRARHRRLSILEM
                     TVEDQTGTIQIVFFNQPFLEELLPAGARVMMTGRVVVGARGWVDRRLEVSQYEVLGED
                     PESPLHVGRIVPIYHETKGWTSRQMRALIRGLLDQHAGSLHEILPVP"
BASE COUNT         1413 a         1829 c         2119 g         1466 t
ORIGIN      
        1 atcatgacgg gtgcaaaagc agtcgtagaa cgggcgctgc aataggtcga tggtatgcct
       61 ctgcgaaacg gactctcaga agaactgaat cgccagggca acgagcattt cgggcgcggg
      121 cactacaccg acgcctatgc gtgctacgcc aaagcgctgg aatgcgatcg catcaccggc
      181 gatcgacggg cgctggcggc gacgctcggc aacatgggaa atatctgcgc cgtaagcgga
      241 cgccgtgaag cggctcagaa ttattatcag gaagtgctgg aattgcagaa aattctcggc
      301 gatgaaaagg ggatcggtac gaccctcggg aatctcggca acctgcgcgc agacgccggc
      361 gagtgggatc gggcaagagc gtattacctt gaggcgctgg atctcatgac caagacgcac
      421 gatgaggccg gcaaggccgt gctattttcc gatctcggcc tggtggcccg tgaaacgaag
      481 cagtttgacg aggccctgag atattacgag caatcgttgg tcttgatgcg gcggctggga
      541 aatcaaggcg gagtcgccga tgcgtggcgc atgatgggcc gaacgttcct gatgcagcaa
      601 cgacacgacg acgcgattgc ttgttgccag accagccaat ccattgcgga acggactcac
      661 gacgagcttc gtgccggcgg ggctcgctat gtgctcgcgc aatgtcatga ggagatgggg
      721 cggctccagg aagcagccga tctcttggag caggttgtcg cgatggatcg gaaataccag
      781 ttgccgaagc tggaagaaaa tacgaagcga ttggaaagtt tgcgcgcacg tcttgccggt
      841 gaagcatccg cggcgtctca gcgccggagt aagagatgag ctgtccgggt tccaccgatt
      901 gggccgacgt ccgtgccgcg ctgaccaaga gcgtcccgca gttttatgag ttggaaccgg
      961 gtggagccct ggcgcttgat ttgggcggcg acgggtggct gttggaagtt cgcccggacg
     1021 ggcacgtgct ctgtcagtac ggcgtctcca tggaggacgt gaagagcttg atgtctgatg
     1081 gaacgccgga agatctgggg accgatgaaa tcgccaagca ggccaaatat tttattcaac
     1141 cagcagtcgc caaattccgt ccacttctgc tgcagtccgg atttgtggag gaaacggaga
     1201 tgaacgaaga gtacgttgcc gtgaccttcc atcggtccgt cgatttccag aacctgtcga
     1261 ccgtgcaagg gcttgtccag tggtgccgcc gctctatcgg agccggctcg tgactggacc
     1321 aagaaaacca atcagcaact tcgaagagct tcgggacgcg ctggcggcct atcggctgcc
     1381 acgggtgatc cttgccgggc tcgagttgga gctgttcaca cgagtcggag acaagacctg
     1441 gaccattcct gaattagcaa aatgtctgca agtgagtgag cggggattgg ccatcctctg
     1501 tcgcaatctg gcgatggccg gtctgctgat caaaaaaggc gcaacgtatc gaaatagtcg
     1561 gttggcggcc acggcgttga atgcgaatca tccggcgtac cgagggagct atctcgatct
     1621 cttgaagaac cattgggccg actgggggag gatgctcgag tcggtgcgga caggattgcc
     1681 gctggatcat gatgtgccgg aagagccgga ttatcgtcgc cgttttacat gggcgatgca
     1741 tcaccgcact ctggacgtag ccccgagcat tgcagcgcag gtttcgctac gtggtgcgaa
     1801 aaccttgttg gatttgggcg gtggaccggg cacctatgcg atggcatttc tggccaagaa
     1861 tccaaatctt cgggctacgg tctgcgatcg tcctgacgca ctggatgtcg cgcgggaaat
     1921 tgctgccaca cacaaagccg gtcgccggat gtcctatctt cctctcgatc tcatgaaaga
     1981 tgagatcccc gggacatttg acgtcgtgtg gtactccaac gtcctgcaca tctattcgcc
     2041 agaagaaaat cagggtgtgt tcaaacgagt ctctgccgcg ttgaatccag gcgggaggct
     2101 gatcattcag gatgcctttc tgcacgaccg ggaaggactg tttcccgaag aagccagcct
     2161 cttcgcggtg tcgatgctac tgtttaccga aaccggcaac acgtactcgg ttatggagac
     2221 cagcgattgg ctgaagcagg ccgggttcac cgccgttcgg ctgctccgga tcaaaaaggg
     2281 gttggaggat tgggagggcg ggatcctgga ggggagagtg tcggcgaaag atgcaagaag
     2341 gaacggccgc ccaacacgat caagaaaaaa ttcgcgaacc cgctgaggcc gctaagtgtc
     2401 acagtcacca tcgctccgtc tgggtgcgtc gcgatactga ccagtgtggc aagatcgagc
     2461 gccaagccga tcgtgccgta gattacacac gccatcgcag accagcgcca cgccatccag
     2521 accgggccga ttaagccgat agggagcaga atcagaaaca cggtccagat gaccttcgtc
     2581 ggagcactcg ctgccgggct gtgagtcaag agaagggtcc ccagtccgat cagaccgagt
     2641 cctcccagca cgatcgcaag gtggagccga tgaggcatgg tgggactata ctgagggcgt
     2701 ttgttctcag caagcgcctg ggcgagtgga gtgtgggggg ggtatggcga tgaccgaaga
     2761 ccgtcgctca acatcagctg gctgtctcct ggtgtacgac ggccagtgcc gtctctgtgt
     2821 gacggcaaag gagggacttg aacgattggg aactcaggat gaagccaagg cggttcggct
     2881 gattccttat caaagcgagg aggcgaagcg cgcgttgggg gctacgtacc gaccgggccg
     2941 cccagacgtg gcgttcctgg tacagccgaa tggggttatt cagcaagggc tcgatgcctt
     3001 tctccctctg ctcccggggt tgaggggagg gcggtttctt gcgactatcc tctctgtccc
     3061 agtgatcaag ccgttcgcct atctcatcta tcgcctggtt gcccgttacc gataccgtct
     3121 gttcggtgaa gtcccttaca ctactgtcga cgatcctgct cagaaaaaga gttctgatcg
     3181 ataagttctc ggtcagattt ggaccctcca tttccgcctg tttgatccct tcggtcaatt
     3241 ctgttctatt cagccccccc tcttttaggg gggaatcttc ctacaccggg gaatttaccc
     3301 aaatagcccg cccacttcag aatgacgacc atgcagggag ggttctctat gggagtcatt
     3361 cgatggagtt gcgggttgat caaatccttg agagtaccga tcctttcccc agttcggaac
     3421 acacagcctc gacccaaaca acaatggaat aggcgatcgc actattcagt cgtgagtggg
     3481 cttctcgtga gtgggttatg cattcctgtg tcggcctgga gcggagatct ttcgggagtc
     3541 catcaagtcg agtttcctac ctctgcgcgt cccgtcgtcg cagcggggtt tgggttccag
     3601 aacgcggaca gttcgacaat cactgtcaga acgtacgacg ccttgaccgg ggagatcctt
     3661 tcggacgagt catacgactt gaacgtcaag gaagaaggga catcatcgtc tcgacaacca
     3721 cgcgagcgga tatttgcggg gggagtcggc ttaggagcag acggactgtc ggattttacc
     3781 ttgcgagtct atgatgcggc gacggggaaa tttctctggg aagggcgatt gaatctgtcc
     3841 gtcaacgatg gtggagccgg ttccacctat cgtattgtcg cccatctcca accgcaaact
     3901 tcgatgagga cgatacgcca agttcagcgt gcaaatggcc agccgtcatt cttattgcgg
     3961 gccaccgatc ccgtgaccgg acagctcatc tgggccgatc agttctcggc tggacgtgga
     4021 atcgttccga ggatcgagcg tatctccgaa ggcgccgccg gtcagccaga aggttctgca
     4081 acgtggttca agaatttcga tttccgaatt cgaatgttcg acgatcagaa tcgacagatg
     4141 atatgggagg atgccattgt ccctgccgac gagaccggag atttgtcgct gggacgtgac
     4201 agcgaagccg atacgctgcc ggcttgggcc aacggtcgga atagcatgag gaaagacgca
     4261 attcgttttg agggattgct tcgttcagcc gaagggataa tgcgaagttc tacgctctag
     4321 ctcttctttg tccgctcgtg caagtgttcc atccaccgag cttgaaactg ttcataggac
     4381 aacgacagtt gcgactgcat ggcaaccggc aaggcttgcc gcgctttcag gtgtgacaga
     4441 agttcgtgca cacggtgcat gccgtagcga tcaatcatgt attggaccgc agagtacgct
     4501 tcgagatagg cgaccgatgc cgcgtcctgc gggagtcggc cccagggacc ctccagcgtc
     4561 ggcaagggga tcaccgtaaa gttctgatgt cgcagttgat cgaggtcctc ccaccgatcc
     4621 gctgaaagcg tcatcgccaa tccctcgtta agccacgtcg gtatcgcagt cgcattgagc
     4681 ccgagttgat cctggagcag ggcgtgcaca aactcgtgtc gaagaacccg ccgtaaccag
     4741 gcttgatctg ttaaggctcc ttgcgtcggc acttggatgc ggccgagtac aggatcatac
     4801 agcccgtcgg cccaggccgg actgcctgtt gtggattgaa atgactctgt cgcatggagg
     4861 acgacgacga tgggtttcga gggaaagtgg ttgagcttct gcccgatctc acggtacgcg
     4921 tcttccagaa tctcgaggac cgttgtccac gtattctggt cggcatctcc atcgtatttc
     4981 acggtgaagt gtgtgctctc ccggctgttc aaccgttgtt cgagttttcc agtctgtcgg
     5041 actttggccg tcaccgctct cagataggat tggatgccgg gatctttgcc ggctctgtcg
     5101 gcggcaagat ccagatggtg cgcggcttcc tccaaccgat cttgttcctg gagcatgtcc
     5161 gcgagtgcca catgcgggaa tggctcgttg ggcgccagcg caatcagttt ctccagatac
     5221 tcgggagtca tcgtcgggtc gcggagttcc caataggcgt gggcaagatt gagctggatc
     5281 gctgggttct tggtgtccag cgaagccgct ttcctgaagg tcttgaccga cgaggcggtg
     5341 ccgcctgatt tttcctgttc gatgcccaga ttgttccaga gcactgcaac gtatgggcgg
     5401 gtcttctcgt tcgacacgag ccaggacgga agatcgccca gtttcatctc cgcttctttg
     5461 acgttgccct tttcgatctc ctcgcgaatc gcttccaaca gttcgcggcc tttggtgtca
     5521 ggcactgcgg tttggtcgat gactcgcgtc tgtttgggct ctggtaccgc gacctcagga
     5581 ggcggttcag ggatgacctc ggaccgagga gggacgaggt gctcggattg tggggcaggc
     5641 gccggtggga gataggtagg cttcagccag gcatggtaca caataaacag tccgagcacg
     5701 catgccacat agaagagtgt ctgcgagatg ttacgccggt acatacccat gcctccgcag
     5761 ttcgagtgta gcaccgtagg tatcctcgac aaagaattct aaggaaaccg gcatacttga
     5821 ttcaggcgga ccgcctcgtt gagcgcaaga gggggctgtg atacgatccc acctcgacat
     5881 gtggcgctcg tccgagccca aacccaccga tccgtttcaa caatggctgg accgtgtttc
     5941 gcgtccgatc gactttgcca gccgtgatgc gtttgcgcat ctccccaccg tgaaaaacct
     6001 caatcggttt gtctcgtcgc aagtggttca agctctctcc gaccgtgtct atccgcgggc
     6061 aattgaaact gcgttactca ggctgcgtga attatttgtc gaggaccgcc aacggctttc
     6121 tacgatcgag caacagcgac ggctacgaga ggccgcgacg attctgagca cattgcgggc
     6181 ggccgcacag gatcctgcgc atgcgtggaa tgagccgcag ggcgtggcta cgtcttcgtc
     6241 gacacggcac ggacctgtct caagcgagct atggaaggtc tcgattcgat ttgcgaaggg
     6301 agtcggtccg caacgaacgg cgctcctgaa tcgcatgggc attgcgacgg ttgaggatgc
     6361 actctggacg gtgccctggc ggtatgaaga tcggtcggtc atcactccga tcgggagtct
     6421 cgcggtcgga gcgcaggctt cgatctgtgg gacgattgtt cacagcaaag cgaagcgcgc
     6481 gcggcatcgg cgtctttcca tcctcgagat gacagtggag gatcagacag ggacgatcca
     6541 gatcgtgttc ttcaatcaac cgttccttga ggaactactt ccggcgggag cccgtgtgat
     6601 gatgacgggt cgggtggttg tcggcgcgcg aggatgggtt gaccgtcggt tggaggtctc
     6661 gcagtacgaa gtgctgggcg aggaccccga atcgccgttg catgtcggtc gcatcgtgcc
     6721 gatttaccac gagacgaaag ggtggacctc gcggcagatg cgggcgctga tccgggggct
     6781 gctcgaccag catgcgggga gccttcacga aatcctgccg gtcccgc
//
LOCUS       DATDNW010000403         5065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_194879, whole genome shotgun sequence.
ACCESSION   DATDNW010000403 DATDNW010000000
VERSION     DATDNW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194879"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VI359_09550"
     CDS             <1..298
                     /locus_tag="VI359_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974538.1"
                     /translation="MFVWSKRLVTALLGVFVLFLGIFLVHGAPDTVSGLKIHTTRWMA
                     LNYVGLVIWVFVWMIDQARMRTQNIWVWLVPFLLAPLPTLMLFVLYLQRRLKPS"
     gene            complement(293..1564)
                     /locus_tag="VI359_09555"
     CDS             complement(293..1564)
                     /locus_tag="VI359_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY6974539.1"
                     /translation="MSIASLVQILANRRIGIMLPLGFASGLPLALTAGTLQAWLTVVG
                     VDLKTIGIFTLVGLPYTVKFLWAPLMDRVTLPWLGRRRGWMLVTQLGVAVGLAFMALI
                     DPRDRPEFLGVLAVLVAFLSASLDIVFDAYRTDVLRPDERGFGAAVWVNGYRFALLVA
                     SAGALVLADRLGWQNTYLLLAALMATGVVTILVSPDPSTTRGAPATLGEAIGGPLKEL
                     FARPAVGSLLALIVLYKIGDAFAASLQTAFLIKGIGFSISDVGYVKGLGLAATLIGAL
                     AGGIAMARLGLVWSLLFFGLLQAISNLGFMWLAWIGKSYAVLMTSVLVENLTGGMGTA
                     AFIALIMSLCDHRYTATQFALLSSLEAFGRVFSGRPSAELVEMVGWIQFFFLSFLIAL
                     PGVWLVWTLRTRLQGCHDLQDADEPSMPGGL"
     gene            complement(1704..2114)
                     /locus_tag="VI359_09560"
     CDS             complement(1704..2114)
                     /locus_tag="VI359_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974540.1"
                     /translation="MNSPQRFGVSFFLSLLFLFPASGGWTKDILPVEPDLNTRVDELY
                     DHEARLFLMLYSLQGNGQVDYVTGRMVQEYARSNFGNPVYQTEAHPLFYWWNHTMWND
                     PEQDGVNGNERIYQENVDFDVLRYKPCTFNGQSC"
     gene            2242..3573
                     /gene="mtaB"
                     /locus_tag="VI359_09565"
     CDS             2242..3573
                     /gene="mtaB"
                     /locus_tag="VI359_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY6974541.1"
                     /translation="MKRASCHTLGCRLNQAETAVLESRLRRDGYRVVEFGEPTDLLVV
                     NTCSVTEDAERACRYVIRKTLRHSPEAFVAVTGCYAQTGSHELRRVPGIDLIVGNQFK
                     WDLPSFLPSPQALKKYARADVIHTRTIDREDFVLPEYGKPDSTRALLKIQDGCNVMCS
                     FCLIPFARGHERSRVVEDVIREATSLVEGGYREIVLTGVNIGQYRQGNVDLVGLIERL
                     ETIAGLERIRISSIEPTTITRTLLSRMTTSSKLCPYLHVPMQSGDDEILDAMNRPYDV
                     AEFTDLIERAVSLIPRLGLGTDLMVGFPGESEAAFTNTLRVAETLPFSYFHIFTYSPR
                     PGTAATKLPHRVSTRVARERAQRIAELSRRKRLIFAEQYIGSVLPVLFESGKTDGLRI
                     GTTANFLKVAVASEIDLTNNLRQVLITGVSDRWAVGQLAEEHRKVRTVPLL"
     gene            3570..4919
                     /gene="miaB"
                     /locus_tag="VI359_09570"
     CDS             3570..4919
                     /gene="miaB"
                     /locus_tag="VI359_09570"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY6974542.1"
                     /translation="MTIYSSPPRIYLETFGCQMNEYDSELVRSLLKREGFVFTEDRER
                     ADVILMNTCAIRENAHNKVYSHLADLKAIKKQRPLVVGVLGCMAQNLKEELTEHQPLV
                     DVLAGPDAYRQLPMLLTSAIRSQKQGLDRKGLAVDLSEYETYDDVVPERNDGVNAWIA
                     VMRGCDNFCSFCVVPYTRGRERSRDPEGIVREAQQIAGQGFKQITLLGQNVNSYRHGS
                     WDFARLLTAVADVSGIERVRFTSPHPKDFPTGLLEAVAGHPHVCKHIHLPMQSGSDRI
                     LGLMNRSYTNREYRALVDRIRALRSDIVLTTDIISGFCSETDAEFAETYKLTEEIRFH
                     NAFIFKYSERKHTIAARKFPDDIPEPVKAERVTRIVDLQRRISHEHNRSYIGTTVAVL
                     VEGSSKRSDSQSTGKTDGNITVVWDNFLIPSQPGDFVTVPIRDASASTLFAHSRPEI"
BASE COUNT         1260 a         1391 c         1310 g         1104 t
ORIGIN      
        1 tatgttcgta tggagtaagc gtctcgtcac ggcactcttg ggagtatttg tgttgttcct
       61 gggcatcttc ctcgtgcatg gcgctccaga cacggtcagt ggcctcaaaa ttcacacgac
      121 gcgatggatg gcactcaatt atgtgggatt ggtgatctgg gtgtttgtgt ggatgattga
      181 ccaagcacgc atgcgaacac agaatatctg ggtttggctc gtccccttcc tcctggcccc
      241 tcttccaaca ctgatgctgt tcgttctcta tctccagcgc aggttgaaac cgtcataagc
      301 cgcctggcat cgaaggctca tctgcgtctt gaagatcatg acaaccctga agtctcgttc
      361 taagggtcca caccaaccat acgccaggca atgcgatcag aaatgacagg aagaaaaatt
      421 gaatccatcc gaccatctca actaattctg ccgatggacg tccggaaaag acccgaccaa
      481 atgcttccag ggacgagagc aaggcgaact gtgtagcggt gtaacgatgg tcgcaaagcg
      541 acatgatcaa tgcaataaat gcagcggtgc ccattccccc ggtcaaattc tccacgagga
      601 ccgaggtcat gaggaccgca tagctctttc ctatccacgc gagccacata aagcccaaat
      661 tggagatggc ctgtagcagg ccaaagaaca aaagcgacca aaccagtccc aatctcgcca
      721 ttgcgatgcc tcctgccaac gcccctatca gcgtcgccgc gaggccaagc cctttcacat
      781 acccaacgtc gctgatcgaa aatccgatgc ctttaatgag aaatgccgtc tgaagagacg
      841 cggcaaaggc atcgccaatc ttatacagca caatgagtgc aagaagactc ccaacggctg
      901 gacgagcgaa gagttctttc agcgggccac cgattgcctc tccgagggta gctggcgccc
      961 cgcgagttgt cgacgggtct gggctgacaa ggatcgttac aaccccggta gccatcaagg
     1021 cagcgagcag cagataggta ttctgccagc caagtcgatc ggctagtacc aatgcaccag
     1081 cactggcaac taatagtgca aagcgatacc cattcaccca cacagcagcg ccgaatcctc
     1141 gctcatcggg gcgcagtacg tcagtccggt acgcgtcgaa cacgatatcg agtgaggcgg
     1201 agagaaatgc caccagcact gcaaggacgc ccaggaattc ggggcgatca cgaggatcga
     1261 tcaatgccat gaaagcgagc ccaacggcta cgcctagctg cgtgaccaac atccagcctc
     1321 gtcgcctccc cagccagggt agcgtgactc gatccatgag cggggcccaa agaaatttca
     1381 ccgtgtatgg gagaccaacg agagtaaaaa tcccgatggt tttcaggtcc acaccgacga
     1441 cggtcagcca agcctggagt gtaccagctg tcagcgctaa tggcagaccg gaagcaaagc
     1501 ctagaggcaa catgatgccg atccgccggt tcgcgagaat ctgaacaaga gaggctatgc
     1561 tcatcaattt tatccggaat gactacaagc tcagcctatc atcagggaag aatccaccat
     1621 cagacatttc ttgaaatcgg acgcgaggag gggccggcca aaaatagaac gagtgatcag
     1681 attaggcaga gtggtctata gcctcagcag gactgtccat taaaagtgca gggcttatag
     1741 cgcaagacgt caaaatcgac gttctcttga tagatccgtt cattcccgtt aacgccgtcc
     1801 tgctcaggat cattccacat ggtgtgattc caccaataga agagaggatg ggcttctgtc
     1861 tggtacactg gattcccgaa attactgcgc gcgtactctt gcaccatccg ccccgtcaca
     1921 taatcgactt ggccgtttcc ctggagagaa tagagcatga gaaacaatcg cgcttcatga
     1981 tcatagagct cgtcgacccg cgtgttcaga tcgggctcaa caggaagaat gtcttttgtc
     2041 cacccacctg atgcagggaa caggaacagt agacttagga agaagctgac gccgaaccgt
     2101 tgcggagaat tcatgctcga cacaccgtcc cgctaaaggc gacaactgag cggatcgtat
     2161 catgagatga tattgtcttc aaccaccgta cgccttgccg caactcgaag aacctctcta
     2221 taatgcccac ccaatcaacg tatgaaacga gcctcttgcc atactctcgg ttgccggttg
     2281 aatcaggccg agacggccgt tctggaatcg cgcctccggc gagacggata tcgtgtcgtc
     2341 gaatttggtg aaccgacgga tttactcgtg gtgaatacct gttccgtgac tgaggatgcg
     2401 gaacgcgcct gccgttatgt tatccggaag acattgcgac attctcctga agcgttcgtt
     2461 gccgtcaccg gttgttacgc ccaaaccggg agtcacgaat tgcgcagggt tcccggtatc
     2521 gatctcatcg tcggaaatca attcaagtgg gatctacctt cgtttcttcc atccccccag
     2581 gcattgaaaa aatacgcgcg ggcggacgtg atccatacca gaacgatcga tcgtgaagac
     2641 ttcgtcctgc ctgaatacgg caagccagac tccacgagag cattgctcaa aatccaagac
     2701 ggctgcaacg tcatgtgcag cttctgtctg attccgttcg cacgaggcca tgaacgaagt
     2761 cgcgtcgtag aggacgtgat ccgggaggcc acaagcctcg ttgagggcgg ctatcgagaa
     2821 attgttctta caggcgtgaa tatcgggcaa taccgtcagg ggaacgtgga tctggtggga
     2881 ttgatcgaac gactggagac gatagccgga cttgaacgaa tcagaatttc atcgattgaa
     2941 ccgacgacga tcacgagaac cctcctcagt cgaatgacca cgtcgtcaaa gctctgcccc
     3001 tatctccatg tgccgatgca aagcggagat gacgaaattc tggatgccat gaatcgtccg
     3061 tatgatgtgg ctgagtttac cgacttgatc gagcgcgcgg tttcgttgat tccccgcctc
     3121 ggactgggca ccgatctcat ggtagggttc ccgggggaaa gcgaggccgc cttcacgaac
     3181 acgctccgcg ttgcggagac ccttcccttc tcctactttc atatcttcac ctattcacca
     3241 aggccaggaa ctgctgccac gaagcttccc catcgggtct cgactcgcgt cgctcgggaa
     3301 agagcccaaa ggatcgcaga actatcacga cgcaagcggt tgatcttcgc ggaacagtac
     3361 attggttccg tcttgcccgt gctttttgaa tcaggaaaga ccgatggtct caggattgga
     3421 acaacagcca atttcctgaa ggtggcagtg gcttcagaaa tcgatctcac aaataatctg
     3481 aggcaggtgt tgattaccgg agtgtcagat cgttgggccg tcggccaatt ggcggaagaa
     3541 catcgaaagg tccgaacggt gcccctacta tgaccatcta ttcctcacca cctcgcatat
     3601 atcttgaaac gttcggatgt cagatgaacg agtatgattc tgaactcgtt cgctcactcc
     3661 tgaagcgaga aggctttgtg ttcaccgaag atcgcgagcg cgccgacgtg attcttatga
     3721 atacctgtgc cattcgcgag aatgcccata acaaagtcta tagtcatctc gccgacctga
     3781 aagccatcaa gaaacagcgt ccattggtgg tgggagtctt agggtgcatg gcgcagaacc
     3841 tgaaagaaga gctcacggag catcaacccc tggtcgacgt gctcgccggt cctgacgcct
     3901 accgccagtt gcccatgctg ctaaccagcg cgattcggtc ccagaaacaa ggattagatc
     3961 ggaaggggtt ggctgtcgat ctctcagaat acgaaaccta cgacgacgtc gtgcctgaac
     4021 ggaacgacgg agtgaatgct tggattgccg tcatgcgggg atgcgacaat ttctgcagtt
     4081 tttgcgtggt gccctataca aggggacggg aacgatcgcg cgacccggaa ggaatcgttc
     4141 gagaagccca acagatcgcc ggccaggggt ttaagcagat caccctcctg gggcaaaacg
     4201 tgaattctta tcgccatggc agttgggact ttgcacggtt gctgacggcg gttgccgacg
     4261 tgtccggtat tgagcgtgtg cgtttcacat ctccgcaccc gaaagatttt cctaccggcc
     4321 tgctcgaagc tgtcgcggga catcctcacg tatgcaaaca tattcatctt ccaatgcagt
     4381 ctggtagcga tcgcattctt ggcctgatga accgtagcta tacgaatcgg gaatatcgtg
     4441 ccttggtcga tcgcattcgt gcacttcgtt cagacatcgt gttgaccacg gatattattt
     4501 ctggattctg ctcggagacc gatgccgagt ttgctgaaac ctacaagctg accgaagaaa
     4561 tccgatttca caacgccttt atcttcaaat actccgagcg gaagcacacg attgcggcca
     4621 ggaaatttcc cgatgacatc cctgaaccgg tgaaggcgga acgtgtcacc aggattgtcg
     4681 atctccaacg tcggatttct catgaacaca accggtcata tatcggcact acggtcgcgg
     4741 tcttagttga agggagttcc aaacgctctg actctcagtc tacgggaaaa accgatggca
     4801 atattaccgt cgtttgggat aatttcctga ttccgagtca gccgggagat ttcgtcaccg
     4861 ttccgatccg cgatgcgtcc gcgtcaacct tgttcgccca ttcgcgacca gaaatctgac
     4921 caatcgactt ccaaaaggct gtagccaaaa tccttcaccg aacaattctg aacagtgaag
     4981 ctcacctaat gtccgtccgg tatcggattt cgaacatatc ctttcctaag aaggtctctt
     5041 ccatctagtc ggttcgggcc caaga
//
LOCUS       DATDNW010000404         9855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_195262, whole genome shotgun sequence.
ACCESSION   DATDNW010000404 DATDNW010000000
VERSION     DATDNW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195262"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VI359_09575"
     CDS             complement(<1..317)
                     /locus_tag="VI359_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974543.1"
                     /translation="MLHRLPPRVVSVLRAIGRLGDTQGMKVYLVGGVVRDLLLHRPIF
                     DLDLTVEGDGIAMARLVSGRFRTSLTVFDRFATARLQFPNGLRVDIATTRKESYALPA
                     ELP"
     gene            complement(376..954)
                     /locus_tag="VI359_09580"
     CDS             complement(376..954)
                     /locus_tag="VI359_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974544.1"
                     /translation="MVLSLFLWVAGSLIFGWGSVFGQLLPPASDDVKLRVTQQELDED
                     AAGRIQKAHGEALAKQFKVDPSVVETLRASKQGWGEIAIRLAMAQELTKTDSKTYPTL
                     AEAMQRIGDLRARKMGWGAIGKELGLALGPVVSEVQRVRQEMRAEAKRTATEGVVTPG
                     EALERSQTERASQSGHADKAQRPRRTEKSQRP"
     gene            1299..1691
                     /locus_tag="VI359_09585"
     CDS             1299..1691
                     /locus_tag="VI359_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974545.1"
                     /translation="MKKKAKKKILPLMVTALAVVAVAAAPAVAKSSKKQGSHSSAAAA
                     AASMGSTPQTQEAPKAGQHDSKPGPAWKTVGGTVKQISGDAYMVEDYEGNQVKLYVGQ
                     GTKQINKKKVGDTVRAEITRGGFANSIQ"
     gene            complement(1797..2552)
                     /locus_tag="VI359_09590"
     CDS             complement(1797..2552)
                     /locus_tag="VI359_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY6974546.1"
                     /translation="MMLKRAVLVAGLLVIAPAFVWAQKGSILEVARFSAGSAGSGLPE
                     GWKPLTFKKVPKQTSYELVKDGGDVVVKAMSDASASGLIKEVRIDPKEFPIVQWRWKV
                     ENLLKRSDVGRKDGDDYPARLYITFEYDPEKVSFAKKLKYKAGQAIFGDIPIGALNYI
                     WESKTPAGTIVDNAYTDFAKMLVVESGPQKVGMWVDESRNIYDDYKRAFGEDPPLING
                     VAIMSDTDNTKERVTAYYGDIVFLQRSTGAAAR"
     gene            complement(2579..4132)
                     /locus_tag="VI359_09595"
     CDS             complement(2579..4132)
                     /locus_tag="VI359_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEY6974547.1"
                     /translation="MDQSESPLVLPNDEFNQQLVRNVHPSNWVNPEPTGRYNIVVIGA
                     GTAGLVTAVIAAGVGAKVALVERHLMGGDCLNVGCVPSKGMIRAARVWSDLKHAEEFG
                     VHIPPGVKYDFGQAMARMRKLRARISHNDSAHRYKSLGVDVFIGSARFAGADTIMVEG
                     PAGDRRLAFGKAAICTGARASAPPTPGLQEAGYLTNETIFWLTELPPRLGVIGAGPIG
                     CELAQAFARFGSQVHLVEAMHGIMPNEDRDAAEIVQKSMQRDGVTILCCGKELTVART
                     ESGKRLTVDSHGRQYGVTVDEILVGVGRTPNVEGLGLDVAGVEYDKTGVRVNERLQTT
                     NPRIFAAGDICSQYKFTHAADAMAQIVIQNALFPHPLGLGYASADSLIMPWCTFTEPE
                     IAHVGLYEADAKKKGIEVETYTFKLDEVDRAILDGEDEGFARVHIRKGTDKILGATIV
                     AARAGDMINEFSVLMKAGLGAKTIAGTIHPYPTQAEVNKKVINLWRKAHFTQRTKNIL
                     IKLFAWMRR"
     gene            complement(4215..4952)
                     /locus_tag="VI359_09600"
     CDS             complement(4215..4952)
                     /locus_tag="VI359_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEY6974548.1"
                     /translation="MIREQVSVTSPMPSTESVSKSNLGKILISLIIACAIGLFFYLDL
                     SRFLSLDALKAHRDQLLVFTEAHYAAAVGLFILTYIVVTGLSLPGAVILTLAGGFLFG
                     SVVGTLYVNLGATTGATLAFLAARYVLRDWVEQKFGKWLGPVQEGFANHAFNYLMTLR
                     LIPLFPFFVVNLVSGLTRMTLGTYVAATALGIIPGSFVYAYAGRQLGTINSLKEIASP
                     NVITAFVLLGALALVPNFYKRFRGKPA"
     gene            complement(5143..5712)
                     /locus_tag="VI359_09605"
     CDS             complement(5143..5712)
                     /locus_tag="VI359_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974549.1"
                     /translation="MRTYCVRFILLSVCVSVIDCGMTFTHASAGSRQQKGQSSPESSG
                     QLKKTLPSPIPQKPIRQKMEQASAGTKAQEISSDTLQRPVKEGAADAQKKARPVHRPR
                     KGSKKAKAQAVIKPHTDLTYHGILENPSRYDPRHNRQTAGTPDPQTPELTHDHFQELD
                     HNRDGKIDPVERAFGRLDMDRDLSARHWQ"
     gene            5944..6588
                     /locus_tag="VI359_09610"
     CDS             5944..6588
                     /locus_tag="VI359_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY6974550.1"
                     /translation="MDQNERLIKTKEQWAKARRGGEEREVFAEGDDRLPPGQHLVETW
                     PVLDLGFKPDIPPSEWKVTIGGFVAHPITWTWEQFLAQPQFKDISDFHCVTSWSRYDN
                     EWEGVSFKHILSVVKPLSLAKFVLFKSYDDYTTNLPLEACDDSDVLLAYQWNGKPLTK
                     EHGGPVRVIVPKRYAWKGAKWVKEITFADKDEKGFWEIRGYSNTALPWNNDRYA"
     gene            6634..7740
                     /locus_tag="VI359_09615"
     CDS             6634..7740
                     /locus_tag="VI359_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY6974551.1"
                     /translation="MPPTSLDICRVECWPVDIPITDPFVVATGARLIAQNIFIRVTLR
                     NGAQGYGEAAPFPEVGGENRESCLAMLQQLSKAVLGRPAADYKAIGRSLSEQAPTHPA
                     ARCGLETACIDAYCHASNMPMWQLWGSADVREYETDITIPICDLAKTLKLAQDWYAKG
                     FRLFKMKVGNDVGEDIRRLEAVHKMCPGITFIGDGNQGFSRKDCLAFANGVKKFGGTM
                     ILLEQPVVRDDLDSMAAIRRETGIPVAADESVRSLIDAREVVARKAADYINIKTMKTG
                     VAEAIEIVSFTKQAGLKLMIGGMVESRIAMGCSFSLVLGLGGFEILDLDTPLLLAGDP
                     VEGGYHYNGSRLVPWQDSGLGMSAVPSRDLMTIE"
     gene            complement(7801..8346)
                     /locus_tag="VI359_09620"
     CDS             complement(7801..8346)
                     /locus_tag="VI359_09620"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY6974552.1"
                     /translation="MVQTKQKDDESAFTLVETMIVVAIIGVLAAVAILSYAHFTKKSQ
                     AVEADIALAEVSRLEEVYFSSTGTYSSDLEAIGFKPNPPLQYYQVSIQAVNGPDGSMF
                     QVIASPLSGADTGPTQSVTRYASNQTNQKTSSGGGVSVGQSVTSTDDSVGNTDSVQSV
                     GGGEPDLSEKMGRTFGPSRRR"
     gene            8915..9289
                     /locus_tag="VI359_09625"
     CDS             8915..9289
                     /locus_tag="VI359_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY6974553.1"
                     /translation="MGVQRPEDIHHLFSSAFNEGNVEAIIALYEPEGALIPQPGQVTR
                     GHTAIRHALAQFLALKGTMQVRTVFVIHGTGIALLRGQWTLTGTGSDGSPVRMVGQSV
                     EVARQQPAGDWLLAIDHPFGAD"
     gene            complement(9295..9675)
                     /locus_tag="VI359_09630"
     CDS             complement(9295..9675)
                     /locus_tag="VI359_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974554.1"
                     /translation="MSASVDLPGAQMRLTITRVATHPFLSRYNLRLTVNGPGNCLAEA
                     ILFPDTGYVSRRNLYMTPAGLLYVVGQFDARVVDGARCTITLAEFRHLERDIVFLGSF
                     DEQEQGRWAFLPAAQRRELPFEKL"
BASE COUNT         2182 a         2827 c         2649 g         2197 t
ORIGIN      
        1 tgaggcaatt ccgccggcag ggcataggac tcttttctcg ttgttgcaat atccactctc
       61 agaccattgg gaaattgcaa gcgcgcggtc gcaaatcgat cgaagaccgt gaggctcgtg
      121 cgaaagcggc ctgagaccag tcgcgccatg gcgataccat cgccctcgac tgtaagatca
      181 aggtcgaaaa tgggccgatg cagcaagagg tcgcggacga caccgccgac aagatagacc
      241 ttcatgccct gcgtatcgcc taaccggccg atggcgcgaa gcacagaaac cacgcgaggc
      301 ggcaggcggt ggagcatact ctttgattgg cgtggcctag gcatcatacg gttacggcat
      361 caagaggcgg gtctcttatg gacgctgtga tttctcagtt cgtcttggtc gctgggcttt
      421 gtcggcgtga cctgattgtg atgctctctc ggtctgtgac cgctccagag cttctccggg
      481 cgtgaccact ccctccgtcg ccgtcctctt ggcttcagct cgcatttcct ggcgcacacg
      541 ttgcacctca ctgaccaccg gtccgagtgc aagtcccagc tctttcccaa tcgcgcccca
      601 ccccattttc cgtgcgcgga gatcaccgat tctctgcatg gcttcggcca gcgtcggata
      661 ggttttcgag tcagtttttg tcagctcctg tgccatggcc aagcgaatcg caatttcacc
      721 ccatccctgc ttggaagccc gaagggtctc gacgaccgat ggatcgacct tgaactgctt
      781 ggccaacgcc tctccgtggg ctttctgtat ccgaccagca gcatcttcgt ccagttcctg
      841 ctgggtgacg cgcagtttta catcgtcaga agcaggaggc agcaattgtc cgaagacgct
      901 cccccatcca aaaatcagcg atcccgccac ccacaagaaa agagacagaa ccatgacctc
      961 gctccttaca gacctagtta aagatcggga cagattgcgc cggtctgggt ggaatgcggc
     1021 tgggattata accaggcgct gaccaatgtg accatgaggc agaaatcggg agtagttgtt
     1081 cctcttccgg atcggaaaat cctttggtcc cgagcagatt tggacagcca tatgccgttt
     1141 tttttccgct ttcattatgg tccccctctg acctccaacc ttgcgactag ctaacccgtt
     1201 gaaattgcag agaacagcca tgccagcctt tcggcacatg ctttgctgaa caccatcatt
     1261 tggccgcaat gaatatcatg atgcaaggag gcattgagat gaagaagaag gccaaaaaga
     1321 aaattctccc gcttatggtt accgctctcg cagtggtcgc tgtggcagcc gccccggcgg
     1381 ttgcgaagag ttcgaagaaa cagggatccc attcctcggc cgctgccgca gccgcgtcaa
     1441 tgggatcgac cccgcagact caggaagccc cgaaagccgg ccaacacgac tcgaagccgg
     1501 gtcccgcatg gaagaccgtt ggcgggacgg tcaagcagat cagcggcgat gcctatatgg
     1561 ttgaagatta cgaaggcaac caggtcaagc tctacgtcgg tcaagggacg aaacagatca
     1621 acaagaagaa ggtcggggac accgttcgcg ccgaaatcac gcgaggcggc ttcgcgaatt
     1681 ccattcagta accccctaac ctgaatccgc gcacccacct tgggcgcggt ggtgtcctct
     1741 cgagggccgg ccccgtacag gccggccctc tcattttgaa cagttgtctc tcaccactat
     1801 cttgctgccg cgcctgtcga tcgctgaaga aagacaatgt cgccgtagta agccgtgaca
     1861 cgctccttcg tattgtcggt gtctgacatg attgccacac cgttgatcaa cggtggatct
     1921 tcgccgaacg ctctcttgta atcgtcgtag atgttgcgtg attcatcgac ccacattccg
     1981 actttctgcg ggccactctc cacaaccagc atcttcgcaa aatccgtata ggcgttgtcc
     2041 acaatagttc ccgccggtgt cttggactcc caaatgtaat tcagcgcccc gatcgggata
     2101 tctccgaaga tcgcttggcc ggctttgtac ttgagtttct tcgcaaaact gaccttctcg
     2161 ggatcgtatt cgaaggtaat gtagagacgg gccggataat cgtccccgtc cttacgaccc
     2221 acatcgctgc gcttgagcag attctcgacc ttccaccgcc actgcacgat gggaaactcc
     2281 ttcgggtcaa tcctgacctc tttgatgagc cctgatgccg acgcgtcgct catcgccttt
     2341 acaaccacgt ccccaccatc cttgaccagt tcataagagg tctgtttcgg caccttcttg
     2401 aaggttaagg gcttccaacc ttccggcagt cctgagcctg ccgaaccagc tgagaatctc
     2461 gccacctcga gtatggaccc cttctgggcc cacacaaaag ctggagcaat caccagcagc
     2521 cctgccacca gcaccgctcg tttcaacatc atttaccggg tcatgtaaga ttaatcgatt
     2581 accgtcgcat ccaggcgaaa agcttgatca gaatgttctt cgtacgttgg gtaaaatgcg
     2641 ctttccgcca caggttgata accttcttgt tcacttcagc ttgtgtcgga tacggatgga
     2701 ttgtccccgc gatcgttttg gcacccaacc cggctttcat caagacggag aattcgttga
     2761 tcatgtcacc cgcacgagca gcgacaatcg tcgcgcccag gatcttgtcc gtaccctttc
     2821 gaatatgaac ccgcgcgaag ccttcatctt ctccgtccag aatggcccga tcgacctcgt
     2881 ccagtttgaa cgtgtaggtt tcgacctcga tccccttctt cttcgcatcc gcttcgtaca
     2941 ggcccacgtg cgcaatttcc ggctccgtga acgtacacca gggcatgatc aatgagtcag
     3001 cactcgcgta cccgagccca agggggtgcg ggaatagggc attctgaatg acgatctgtg
     3061 ccatcgcgtc cgcggcatgc gtaaacttgt actgtgaaca gatgtcgccg gcggcaaaga
     3121 ttctcgggtt tgtggtctgc agccgttcat tcaccctgac gccggtcttg tcgtactcga
     3181 caccagcgac gtcgagcccg aggccctcta cgttcggtgt acgtcccacg ccaaccaata
     3241 tctcatcgac cgtgacgccg tactggcgcc cgtgcgaatc gactgtcaac cgtttcccgc
     3301 tctctgttcg tgccaccgtc agctcttttc cgcaacagag tattgtgacg ccatcccgtt
     3361 gcatcgattt ctgaacgatc tccgcggcgt cgcggtcttc gttcggcatg atgccgtgca
     3421 tggcttcgac gagatggacc tggctgccaa accgggcaaa tgcctgcgcg agttcgcagc
     3481 cgattggccc ggctccgatc actcccaagc gcggcggcag ctcagtcagc caaaagatcg
     3541 tttcgttcgt gaggtaacca gcttcctgta atccaggcgt gggaggggct gatgcccgcg
     3601 ctccagtaca gatcgcggcc ttaccaaaag cgagccttcg gtcgccggcc ggcccctcga
     3661 ccataattgt atcggccccc gcaaatcgtg cactcccgat aaagacgtcc acccccagcg
     3721 acttataccg gtgcgccgaa tcattgtgac tgatccgcgc tcgtagtttt ctcattcgag
     3781 ccatagcctg gccgaagtcg tatttcaccc ctggaggaat gtgcacacca aattcttccg
     3841 catgcttcaa atccgaccac acccgtgcag ctcgaatcat gcctttcgac ggaacgcagc
     3901 cgacattgag acagtcaccg cccatcaaat gtcgttcaac gagggccacc ttcgcgccga
     3961 cgccggccgc aatcaccgcg gtcaccagcc cggcggttcc tgcgccgatc acaacgatat
     4021 tgtagcgacc ggttggctcc ggattgaccc aattggacgg gtggacattc ctgaccagct
     4081 gctggttgaa ctcatcgttc ggcaatacca agggagattc agactggtcc atgtattcaa
     4141 tcctcttagt cgattgagtg atcatagcgc gcagtcctgt gttttcgaaa gatgggttga
     4201 cgaagaaaaa cccgtcatgc cggtttccct cggaatctct tatagaagtt cggcacgagt
     4261 gccaatgctc cgagaagcac gaacgccgta atgacgttgg gagatgcgat ttctttgagt
     4321 gaattgatag tcccaagctg tcggcctgcg tatgcgtaga caaacgagcc gggaatgatc
     4381 ccgagcgcgg tcgccgcgac atacgtcccc agagtcattc gcgtgaggcc tgacacgaga
     4441 ttcaccacaa agaacggaaa gagcggaatc aaccggagtg tcatgagata gttgaaagca
     4501 tggttcgcaa aaccctcttg tacgggacct aaccacttgc cgaatttttg ttcgacccaa
     4561 tcgcgcagca catagcgcgc ggcgagaaag gccaaggtcg ccccagttgt ggcgccaaga
     4621 ttgacataca acgtcccgac cacgctcccg aagagaaatc cgccggcgag tgtgaggatc
     4681 acagcgcctg gcaacgacag ccccgtgacg acaatatacg tgaggataaa taatccgacc
     4741 gcagcagcat agtgagcttc ggtgaagacc agcaactggt cgcgatgtgc tttcaatgca
     4801 tccaacgaga ggaatcgact gaggtcgaga tagaagaaga ggccgatcgc acaggcaatt
     4861 atcagggaga taagaatttt cccaaggttc gatttgctga ctgactcagt cgatggcata
     4921 gggctggtca cggagacttg ctcgcgtatc atgggggctc ctttcctcac tgtcaatgag
     4981 gtttagtatc cgatcagtcg attgattcct ccgatcctta cggcctcaca aacaagctcg
     5041 tccgaagcgg attccgagtg ggagcctagc tcctgtagga cctagcacat gaggtggata
     5101 tggggaaacg aacgccgaga ggtaggtgat acgtcagagc ggctactgcc aatgccgagc
     5161 agaaaggtct cgatccatgt cgagccgtcc gaacgctctc tcaacagggt cgatcttccc
     5221 gtcgcggttg tgatccaatt cttggaaatg atcgtgggtc agttccgggg tttgaggatc
     5281 cggcgtgcca gccgtttgac gattatgtcg tgggtcgtag cgtgacggat tttcgaggat
     5341 tccgtggtac gtcaggtctg tgtgtggctt aatcactgcc tgagcctttg ctttcttgct
     5401 tcctttgcgt ggccgatgca ctgggcgagc cttcttctgg gcatcagcag caccttcttt
     5461 taccggtcgc tggagcgtgt cggacgagat ttcttgagcc ttggtaccag cagaagcctg
     5521 ctccatcttt tgcctgatcg gcttttgggg aatgggggat ggcaaggttt tcttcagttg
     5581 gcctgatgat tcgggggaag actggccctt ctgctgccga gatcctgcgg atgcatgcgt
     5641 gaacgtcatc ccacagtcga tcacggagac gcagacgctc agcagaataa acctgacaca
     5701 atatgtacgc atgtcctctc cagttccaag accttcagta agtgtatcag gtaggatggg
     5761 caatggaggc accgtaagat cccgatttac aaacgaattc ggtcatgacc accttcctgt
     5821 cgaacaggag gctgtcgcgt tctttctatt tcccttcatc aggctcaatc taggatggcc
     5881 tatttgctag cttgtaaggt tggcctggtt tgccctactc tcctctcaca ggagaacaca
     5941 tccatggatc aaaacgagcg gctcatcaag accaaagagc agtgggcgaa agcacggcgt
     6001 ggcggcgaag agcgcgaagt tttcgcggaa ggtgacgacc gactcccgcc gggacaacac
     6061 ctggtagaaa cctggccagt gttagacctt gggttcaaac ccgacattcc tccctcggag
     6121 tggaaagtga ccatcggtgg cttcgtggct catccgatca cgtggacctg ggagcaattc
     6181 ctcgcccaac cgcagttcaa ggatatctcg gattttcact gcgtcacgag ttggagccgc
     6241 tacgacaacg aatgggaggg cgtcagtttc aaacacattc tctccgtcgt gaagcctctc
     6301 tcactcgcga agttcgttct ctttaagtcc tacgacgact atacgacgaa cctgccgctc
     6361 gaagcctgtg acgacagtga cgtactgctg gcctatcagt ggaatggcaa accgctcacg
     6421 aaagagcacg gcgggccggt gcgtgtcatc gtcccgaaac gctacgcgtg gaaaggggcg
     6481 aagtgggtga aggagatcac gttcgcagac aaggatgaga aaggattctg ggagatccgc
     6541 gggtattcca acacagcatt gccctggaac aatgaccgct atgcataagg acgaactatt
     6601 ctctaatcga tcgcttcttc gcgcaatgct gtgatgccgc ctacctcact cgatatctgc
     6661 agagtggaat gctggcccgt cgacatcccc atcacggacc cgttcgttgt cgcaacgggg
     6721 gcacgcctga tcgcacaaaa tattttcatt cgcgtcacgc ttaggaacgg tgcgcaaggg
     6781 tatggggaag cagcgccttt ccccgaggtt ggtggagaga atcgcgagag ttgtcttgcc
     6841 atgcttcaac agctcagcaa ggctgtcctc ggccgtccag ccgccgacta taaagccatt
     6901 ggacgatcgc tatccgaaca agcaccgact catcccgccg cacgttgtgg gttggaaaca
     6961 gcctgtatcg acgcgtattg tcatgcgtcc aatatgccga tgtggcaact ctggggaagc
     7021 gcagacgtcc gtgaatacga aactgacatt accattccca tttgtgacct cgccaaaacc
     7081 ctgaagttgg cgcaggattg gtatgccaag ggctttcggc tgttcaaaat gaaagttggg
     7141 aacgatgtgg gggaggatat tcgacggctc gaagctgtcc acaagatgtg tcccggcatc
     7201 accttcatcg gtgacggcaa tcaggggttt tcacgcaagg actgtctcgc gtttgcgaac
     7261 ggcgttaaga agtttggagg cacgatgatc ctgcttgaac agccagtcgt gcgtgacgat
     7321 ctcgacagca tggccgcgat tcggagagag acagggattc ccgtcgctgc ggatgaatcg
     7381 gtccgctcgc tgatcgatgc tcgggaagta gtcgcccgaa aggcggccga ttatatcaat
     7441 atcaagacca tgaagaccgg tgtcgcagaa gccatcgaga tagtgtcttt cacgaaacag
     7501 gcagggctca agctcatgat cggcggaatg gtcgaatcga ggatagccat ggggtgctcg
     7561 ttcagtctag tgctgggctt gggagggttt gaaattctcg atctggatac gccattgctc
     7621 ttggcagggg atcctgtcga ggggggctat cactacaacg gatcccgact cgtgccatgg
     7681 caggattcgg gtctcggcat gagcgctgtt ccaagtcgag atttgatgac tatagaatag
     7741 gattgggtcc ccattgggct aaatgtgaac gtgatcagca ttccatctct caaatatggt
     7801 tcagcgtcgc ctggatggac caaaagtcct acccattttt tcactgaggt ctggttctcc
     7861 tccaccaact gattgcacac tatcggtatt tccgaccgaa tcatcagtag acgtcacgct
     7921 ttgtccgaca ctgactcctc cgccactgga tgtcttctga ttcgtttggt tgctcgcgta
     7981 tcgtgtgact gactgtgttg gaccagtatc agcgcctgat agaggactcg ctatcacctg
     8041 aaacatcgac ccatccggac cgttcaccgc ttgaattgag acctggtagt actgtagtgg
     8101 cgggtttggc ttgaatccga tggcctcaag atctgatgag tatgtgcccg tcgaggaaaa
     8161 atatacttct tcgagacgac tgacctcggc caatgctatg tctgcctcaa cagcctgaga
     8221 ctttttagtg aaatgcgcat acgaaagtat ggcgactgcg gccaacactc caatgatggc
     8281 cacaacgatc atcgtttcta ccaacgtgaa ggctgactcg tcgtctttct gcttcgtctg
     8341 taccatcgcg ggataccgaa tcgtgaggat tcaatgagcc tttgacagtt cgaggaagcg
     8401 taccattatc cgggaaagag tcgaagcact tcatgagttc tcttcacact tcaagcaatt
     8461 gagtagttgg ccgagatggg ctctccgacc atcttcggta aggacccatg gacggctggt
     8521 gagaggcggc tgatggcgca cgtgctgccc gtgaccacag gccaggtcag ccacccaatc
     8581 cccttgttca tctaagtgga acccgataat cgcctgcttc atgacagttt gaatccatca
     8641 aggctgacac ctgctgatca cctgtgatgc tcgccgataa gaccagatct tgatctgaaa
     8701 acgctttgca gcaaccttca tcgcaccaag atcccatcac tcaccgttga ttgactcttc
     8761 ccaccaacca ggagcatggt atcgcgtcca ccgtgcacag aagaaaactt ctcgtggcaa
     8821 gccttctgcg gtgctccgtc tcgacgttgt tatgcactgg cctcatgtgc tgcgacggag
     8881 cacggtaccg gccctgtaac gaaaggagcc tgtaatgggt gtgcagaggc ccgaagatat
     8941 ccatcatctg ttttcatcgg ccttcaatga gggaaacgtg gaggcgatca tcgccctcta
     9001 cgagccggaa ggcgcgctga tcccgcagcc aggccaggtc acacgcggtc ataccgcgat
     9061 ccgccacgcc ctcgcacaat tcctggcgtt gaaaggaacg atgcaggtgc gaaccgtgtt
     9121 tgtgattcac ggcaccggca tcgccctgct gaggggtcag tggacactca ccggaaccgg
     9181 ctctgacgga agccctgtca gaatggtcgg acagagcgta gaagtcgccc gtcagcaacc
     9241 agccggagat tggctcctgg cgattgatca cccattcggt gccgactgag gcactcagag
     9301 tttttcaaac ggcaactctc gccgttgtgc tgcaggcaga aatgcccatc gtccctgttc
     9361 ctgttcatcg aaactgccga gaaacacgat gtctcgttca agatggcgaa actcggccag
     9421 cgtaatcgtg catcgcgcac catcgaccac acgggcgtcg aactgaccga cgacataaag
     9481 cagacccgcg ggagtcatat aaaggttccg ccggctcacg taccccgtat ccggaaacag
     9541 gatggcctcg gccaaacagt tcccgggacc gttcaccgtc aatcggaggt tgtagcggga
     9601 aagaaacggg tgagttgcca ctctcgtgat cgtcaggcgc atctgcgccc ctggcagatc
     9661 gacggaggcc gacacaggca catcactgtt gcaggcaaac agggagagaa taagacacag
     9721 accgcctttc gttccgtgag gccgtttcac ttggccggta ctttgggcgc agcgccgatc
     9781 gcatcgagcc ctgcctgtgc acaggcatca tcgagatggg cacctggtgc tccaccgact
     9841 ccaatcccac cgact
//
LOCUS       DATDNW010000405         4751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_682330, whole genome shotgun sequence.
ACCESSION   DATDNW010000405 DATDNW010000000
VERSION     DATDNW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682330"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VI359_09635"
     CDS             <1..298
                     /locus_tag="VI359_09635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974555.1"
                     /translation="AEIYRVLRPGGRFVCLEFSRPVRGWLRTLYDWYSFRLLPWIGTK
                     VARDTTGVYEYLPASIRTFPDQERLCQALRDAGFRQVEYRNLTGGIVAIHVAMK"
     gene            361..1989
                     /locus_tag="VI359_09640"
     CDS             361..1989
                     /locus_tag="VI359_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6974556.1"
                     /translation="MNSILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRIVDLSLFP
                     DSQRLQAVRAAAIEFKPDLVCFSWRDIQIFSPHEGDSSLEHAFNFYFASNPLKRVVAS
                     FEGLKQLYRYYSHIRANLSYPWLIAKEFPKTQIMIGGGAFTAFADQLIEKLPEGTIGI
                     LGEGEDAILKVLDGQSLDGERYIVREGKTIRKGQQAAPALLDALTVDLPYLTSIFPQY
                     RDYIDESIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPAMVVKDISQHYHQWGARRFW
                     FTDAQFITGKESYPQCTEILERILAEKLEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEDSLLQS
                     VESYKTVASILGEERVFPLMFFLGIQPNTDLEHRLLEEGYLSAGYNPLMLTPNSIRKL
                     LYNPAPLNKIIAKACLLAWERKRGSRDPRAWSGSLSQATEASGKYADKSLLRGIDGNS
                     GRDALLSLEEIIRSRKSSSQSMASQENPAVTSAR"
     gene            2148..4244
                     /locus_tag="VI359_09645"
     CDS             2148..4244
                     /locus_tag="VI359_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY6974557.1"
                     /translation="MYKTIYVPVDNSDHSNTAVDVGVHLAKTFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVVRRVRNSDMLI
                     IKNTQPMTGGKIVVAVDGSPYSFGGLMTGLALGKAFNMPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKDIPALLIVGRIGVHSDEDMDIGSNTENLLRSAPCNVLVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNTVVDAAVGHI
                     LPKGAMEAMRALGGSLDAAGIDRDKMQADDAVAKDLMGSTLSGMMTQVVEEKPKNSEA
                     TQAYLDRMSQNYFVCDGCGYIGKGDTPVKCPVCGADGARFKQVDKSIFEAAAKAEGEI
                     ETDVAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKAKIEKSARKLGMTTITLEYAAPMI
                     KEAADEDYQPIFANKGTGTSVEAEAKLAGANGNGTNGNGAHGNGNGNGHAEAPSPYTW
                     TADAQARLERAPEGFMRDCTRALIQKHADKLGTTHITLEVANEGIEQAKGYMAEAMKT
                     GNLKDMIANLTGKGTA"
     gene            4250..>4751
                     /locus_tag="VI359_09650"
     CDS             4250..>4751
                     /locus_tag="VI359_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY6974558.1"
                     /translation="MGKSLPILNAPSLAGAFGAIQQQVTQFFSPSPTGSSPNDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAGGGNDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARHAVKLGFMVVFGTNGMLINDKMAKTLVEIGVMGVGISIDS
                     LDPAKHN"
BASE COUNT         1145 a         1338 c         1320 g          948 t
ORIGIN      
        1 tgcagaaatc tatcgcgttc tgagaccagg agggcgtttc gtgtgcctgg agttttcccg
       61 gccggtgagg ggctggctcc ggacgctcta cgactggtat tccttccggc tcctgccctg
      121 gatcggtacg aaagtcgcgc gagacacgac cggggtgtat gaatacttac cggcttcaat
      181 ccggacattc cccgatcaag aaagactctg ccaagccctg cgtgatgcag gcttccgtca
      241 ggtggagtat cggaatctca ctggcgggat tgtggcgatt cacgtggcga tgaagtagcc
      301 taaaggctgt cagctttcag catcaggagc tgacggctga ctactgagag ctgattgctg
      361 atgaactcga ttctctacat cttcctccct tgcaagaaag tctacccgat cggtgtgacg
      421 tatctggccg actttatcca ccgacgtaag ccggaggtcc gccagcgcat cgtcgacctt
      481 tcgttgttcc ccgattcgca acgattgcaa gccgtccgcg ctgcggccat cgaattcaaa
      541 ccggacctgg tctgcttctc ctggcgcgac attcagattt tctcgccgca cgagggggac
      601 tcatcgcttg aacacgcgtt taatttttac ttcgccagca acccgctcaa acgtgtcgtt
      661 gcatcgttcg agggattaaa gcagctgtac cgctactaca gccacattcg ggccaatctt
      721 tcttatccct ggttgatcgc gaaggaattc ccgaagacgc agatcatgat cggcggcgga
      781 gccttcacgg cctttgccga ccaattgatt gaaaagctcc ccgaaggaac catcggcatt
      841 ctcggtgagg gggaggatgc gatcctcaag gtcctagatg gccagtcgtt ggatggcgag
      901 cgttatatcg tgcgtgaggg aaaaacgatt cgcaaaggac aacaggctgc gcctgcactc
      961 ctggatgccc tcaccgttga tctcccgtac ttgacatcga tttttcccca atatcgtgac
     1021 tacattgatg agtcgatcgg ggtgcaaacc aaacgcggct gcccctacga ctgtgccttc
     1081 tgcctgtatc cctacatcga agggaaacgg gtgcggtacc gcccgcccgc gatggtcgtg
     1141 aaagatatct ctcaacacta tcaccagtgg ggcgcacgtc gtttctggtt caccgacgcc
     1201 cagttcatca cggggaaaga atcctatccc caatgtacgg aaatcctcga acggatcctg
     1261 gctgagaagc tggagatcga atggtccggc tatatccgca cgtcgttgat tacgccggag
     1321 ttggcgaagc tcatggttcg atccggagtg ggcgatctgg aagtcgcgat cacatccggg
     1381 tctcaagagg tgctgaacaa tttgcacatg ggcttcaagc tggaacgact ctatgacggc
     1441 tgccgctatc tcgcggaggc ggggttcaag ggaaaggtga tcctgaacta ttcactgaat
     1501 tcaccgaaag aaaccgagga cagcctgctg caaagtgtcg agtcctataa aaccgtggcg
     1561 tcgatcctcg gtgaagagcg ggtattccca ttgatgttct tcctcggaat ccaacccaat
     1621 acggaccttg aacaccggtt gctggaagaa ggctatctct cggctgggta taaccccttg
     1681 atgctcaccc caaacagtat ccggaagctc ctctacaatc cggctccgct caacaagatc
     1741 atcgccaagg cctgccttct ggcctgggaa cggaagcgag gcagccggga cccgcgggcc
     1801 tggtccggtt ccttgtcaca agccaccgag gcgtctggga aatatgccga taagagtctg
     1861 ctgagaggaa tcgacgggaa ctcaggacgg gatgcactcc tctctctcga agaaattatt
     1921 cgttcacgaa aatcttcatc tcaatcaatg gcttcacaag aaaatcctgc ggtaacttcg
     1981 gcccgttagt cccccatcca tcggtcccca atgtccttgc attcggtcgg aacccagtgc
     2041 tatgatgcgc cctcttttga ggctggggat tggtgtcaac cgtttggatt gtttgacgaa
     2101 tctcgcaatc gacgccagcc acgagagcgc ctggaggagg catctcaatg tataagacga
     2161 tctacgtacc ggtcgataat tccgaccatt ccaacaccgc ggtggatgtc ggagttcatc
     2221 tcgccaaaac cttcggctcc aaaatcgtgg gcagccacgt gtatgccgcc aagatgcacg
     2281 acaagcgatt caaacagatg gaagcaggtt tgccggagga ataccacgat gagaaagagt
     2341 tggaccggca acgacagatc catgactcgc tgattactcg tggactccag atcatcaccg
     2401 attcctacct agactacgtc gacaagaaat gtaatgaagc caacttgccc gtcgaacgcc
     2461 gatccctcga aggacgcaac tggaaggtac tggctgaaga tatcaacacc aacgcctatg
     2521 atctcgtcat catgggagcc ctgggcgtcg gcgcggtgaa agacagcgtg atcggcagca
     2581 atacggagcg cgtggtccgg cgcgttcgca actccgacat gttgatcatt aagaacacgc
     2641 agccgatgac cggcggcaaa atcgtcgtag cggtcgacgg cagtccctat tcattcggtg
     2701 gactcatgac cggccttgca ctcgggaagg cctttaacat gccggtcgaa gccatttccg
     2761 ccttcgatcc ctattttcac tatgccgcgt tccacagcat ctccggcgtg ttgaacgagg
     2821 aagcgggcaa ggtgttccgt tttaaggaac aagaaaagct tcatgaggaa atcatcgaca
     2881 gcgggctcgc aaagatctac cagtcacatc tcgatatttc gcgcgagatc gcccaggccg
     2941 aacagaccga cgtcaaaacc acgctacttg acgggaaagc cttcgaaaaa atcattcaat
     3001 atgtccgcaa ggatattccg gccctcttga ttgtcggccg catcggtgtc cacagcgatg
     3061 aagatatgga catcggcagc aacacggaga atctcctccg gagcgcgccc tgtaatgtcc
     3121 tcgtctcgaa tcggaaatat gtgccgccga tcgataccca agccgaatac acgatcgcct
     3181 ggaccgaaga agccttacgg cggatggaga agattccggt gtttgcacgg ggcgtcgcca
     3241 agacggcgat tcatcgctac gccatcgaaa agggccacac gattattagc aacacggtgg
     3301 tcgatgccgc tgtcggtcat atcctgccga aaggtgcgat ggaggcgatg cgcgcgttgg
     3361 gcggcagcct cgacgccgcc ggaatcgatc gagacaagat gcaggcggac gatgcggtgg
     3421 caaaggacct tatgggatcg actctgagcg gcatgatgac gcaggtggtc gaggaaaagc
     3481 cgaagaactc ggaagctacg caggcctatc tcgaccgcat gagccagaac tacttcgtgt
     3541 gcgacggctg cggctacatc ggaaaaggcg acacgccggt taaatgtccc gtctgcggag
     3601 cggacggcgc tcgcttcaaa caggtcgaca aaagcatctt cgaagcggcc gccaaggcgg
     3661 aaggcgagat cgaaaccgat gtggcctatg acgacgtccc gatgcaatgg acaaaggacg
     3721 cgaaggaagc gattcgagcc gttccggccg gtttccaacg tcgtcgcgcc aaggcaaaga
     3781 tcgaaaagag cgcgcgcaaa ttgggcatga caaccattac gctcgaatac gccgcgccga
     3841 tgatcaaaga agcagcggat gaagactacc agccgatctt tgccaacaag ggcaccggca
     3901 cctcagtaga ggcagaagcc aagctggctg gtgcgaacgg aaatggcacg aatggcaacg
     3961 gggcccacgg caacggtaat ggaaatgggc acgccgaagc accctcgccc tatacctgga
     4021 cagctgatgc acaggcgcgg ctcgagcggg caccggaagg attcatgcgt gattgcacgc
     4081 gtgcgttgat tcagaagcat gccgacaagc tcggtacgac tcatatcacg ttggaagtgg
     4141 caaacgaagg catcgagcag gcaaagggct acatggccga agccatgaag accggtaacc
     4201 tcaaggacat gattgccaac ctcaccggga aaggcacggc ttagccgaca tgggcaagtc
     4261 actgccgatc ctcaacgccc catcactcgc tggggcgttc ggagcgatcc aacagcaagt
     4321 cacgcaattc ttcagcccgt caccgacggg gtctagccca aacgacggac ggaccgtcga
     4381 cgatttcaaa ccttatctcg tcgcgctgaa cctaacaaag cgctgcaacc tcaaatgcga
     4441 ccattgttac ctcgatgcca ccacaaaagc aggcggcgga aacgatgaac tgtccacaga
     4501 agaatgtttc cgtctcattg atcagatcgc ggaagtgaat aagggctgtc tgttagtcat
     4561 cacgggtggc gagccgctgg ttcgccccga catcctcgac atcgcccgtc atgccgtgaa
     4621 gctcggcttc atggtcgtct tcggtaccaa cggcatgctg atcaacgaca agatggccaa
     4681 gacgctggtt gagatcggcg tcatgggtgt cggcatcagc atcgattcac tcgatccggc
     4741 aaagcacaac a
//
LOCUS       DATDNW010000406         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_539602, whole genome shotgun sequence.
ACCESSION   DATDNW010000406 DATDNW010000000
VERSION     DATDNW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539602"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /gene="mutL"
                     /locus_tag="VI359_09655"
     CDS             complement(<1..1369)
                     /gene="mutL"
                     /locus_tag="VI359_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY6974559.1"
                     /translation="MSATGCATKIHVLPGDVISRIAAGEVIERPAAVVKELIDNSVDA
                     ASRHVIIDIKESGLGLIRVTDDGEGMSRIDLPLAFERHATSKLRSDRDLVAIATMGFR
                     GEALPSIASVSNVMVMTATRQDTIGAQLALCGGVAQPVVDAPAVPGTRIEVTDLFHHQ
                     PARKKFLKSPATEFSHISHVVQQAALAWPSVHFRLIHNGQDVLNYPAAMSDRDRILQV
                     YGSAFVDHTVEVRGRIAGLAVNGYIIDPVHARTSRQPQDLFVNRRPVRNATVFHAVTD
                     GYGSFLPKGHYPTFVLFVDIDPDRIDVNVHPMKKEIRFAETEAVHRLVRHTIRHALGG
                     EERKTVLGLGQPAGLRGAADLGDVAATPDGSSATVGAETGRRPPESCSSLPSELGSVE
                     GSQLVIAENNAEPYRRVAEDEIVPLGQLHRTYLVAQVGHELHVIDQHTAHERVLFERL
                     WRNWQG"
     gene            complement(1436..2197)
                     /gene="ybgF"
                     /locus_tag="VI359_09660"
     CDS             complement(1436..2197)
                     /gene="ybgF"
                     /locus_tag="VI359_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6974560.1"
                     /translation="MCAQRSVLVLVVIGLVATGCARHADFVDLRDHLATVSKTQDQDH
                     QRLDAMQRRLESVERVKDAEPVKPRLDELNARLQKLESRLAKMEEAQGQPAAKVEPAP
                     VEPVRPTKPQKPGSAGDMQTIVPGVPAITPTSAFNLAYNDYLNGKYDLAVAGFQRFVK
                     DFPGTSLTPNAYFWLGESYYQQKDYVRAMQTFEYVATEYPGNEKVPAAIYKLGLASAE
                     TGDIAKSKKNLKRVIEEFPTSDEAKLAKNRLAEIR"
     gene            complement(2215..>3393)
                     /gene="ybgF"
                     /locus_tag="VI359_09665"
     CDS             complement(2215..>3393)
                     /gene="ybgF"
                     /locus_tag="VI359_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY6974561.1"
                     /translation="TTTRYAWIQKSLETQDLKNKEDRDRLRMEVNARLDDVNKQMELL
                     RKDIIEAVQKTNSALTKNIDARLEEQHKATLENQNRTEQLVAKFTQFNQSLTAFRESL
                     SGLSERVAQDEQSTKTFVNKLEADSKASTAYVNEMNKSVSGHLGDVNKSVTSVAAVTQ
                     KLAARIDEQDRRLELMAKSVDQVAQDAHARAGGKGATRQSPSKPAHRSAYAPQAEPQK
                     SEPPGEPEQAETQPQAIAEPVRSAQVVVAPDEPDDEAKPQVSPEAARPDKTEYERLLS
                     LFKDGNLVGAERGFAAFLTSYPNSDLAPNARYWLGEAQYGQKDYKEAISSYDRVELDY
                     PKSEKVPAALLKKGYAYLALKDKKRASSAFKQVVTLYPKSPEAGKASDKLTQMTKESR
                     "
BASE COUNT          647 a         1028 c          932 g          786 t
ORIGIN      
        1 gcccctgcca attccgccag agtcgctcga agagcacccg ttcatgcgcc gtatgctgat
       61 cgatcacgtg cagctcatgc ccaacctgag ccaccagata cgtgcgatgg agttggccaa
      121 gaggaactat ttcatcctca gcaacgcgcc ggtacggttc agcattgttc tctgcgatta
      181 ccagctgaga tccttcgacg gaaccgagtt cggacggaag actcgaacat gattcgggag
      241 gcctacgacc cgtttctgct ccgacggtcg cagatgaccc atccggagtc gcagcgacat
      301 cgccgagatc ggcggccccg cgcaagcctg ccggttggcc caggccgagc acggtctttc
      361 gttcctcacc gcccagcgcg tggcgaatcg tgtgccggac cagccgatga accgcttccg
      421 tctcggcgaa gcgaatttcc tttttcatgg ggtgaacgtt cacgtcaatc cgatcaggat
      481 cgatgtccac aaacagaacg aaggtcggat aatggccttt cggaagaaac gatccgtaac
      541 catccgtgac agcgtgaaac acggtggcgt ttcgaaccgg tcggcggttc acgaacaaat
      601 cctgcggctg tctcgacgtc cgggcatgaa ccggatcgat gatatagcca ttgaccgcca
      661 gtcccgcgat ccggcctcgt acctcgacgg tatgatcgac aaaagcggag ccgtacacct
      721 gtaagatgcg gtcgcgatct gacattgccg ccggatagtt gagcacatcc tgtccgttat
      781 ggatcagccg gaagtgaacg gacggccatg ctaacgccgc ttgttgtacg acatgactga
      841 tatgagaaaa ctccgtcgcc ggcgacttga ggaatttttt ccgcgcaggc tgatgatgaa
      901 acagatccgt cacctcgatc cgcgtgccgg gcactgccgg cgcatccaca accggttgag
      961 ccaccccgcc gcacagcgcg agctgcgcac cgatcgtatc ctgcctcgtc gcggtcatga
     1021 ccatcacgtt cgaaacggag gcgatgctcg gcaacgcttc accccgaaat cccattgtgg
     1081 cgatggcgac aaggtcccgg tccgagcgca acttgctggt cgcatgacgc tcaaacgcca
     1141 acggcagatc gatgcggctc atcccttcgc cgtcgtcagt gacgcggatc agtccgagtc
     1201 cactctcttt gatgtcgatg atgacatggc ggcttgcggc atccacactg ttgtcgatca
     1261 actctttgac gacggcggct gggcgctcaa tgacctctcc cgcggcaatg cggctgatga
     1321 cgtctcccgg caggacgtgg atcttggtcg cgcaacccgt ggcggacatg gagctagact
     1381 cctcggaggg cacgcgacgg gattcgtgtc tgggtccgtc gcgtctggct ggcagtcatc
     1441 gaatttcagc caatctgttc ttcgctaact tggcctcgtc cgatgtcgga aactcttcga
     1501 tcactcgctt gagattcttc ttggacttgg caatatcccc tgtttcagca gaggctaatc
     1561 cgagtttgta gatcgcagcg ggtaccttct cattccccgg atactcggtc gcgacgtatt
     1621 cgaacgtttg catcgcccgc acatagtcct tctgttggta ataggattcc cccaaccaaa
     1681 agtacgcatt gggcgtgagt gaggtcccgg gaaagtcttt cacaaagcgc tgaaaacccg
     1741 ccacagccag gtcatacttg ccgttcagat agtcgttgta cgcgaggttg aatgcggacg
     1801 tgggggtgat cgccggaacg ccgggcacaa tggtttgcat gtcgcccgcg ctcccgggct
     1861 tttgcggctt cgtcggacga acaggctcga ccggagccgg ctcaactttg gctgccggct
     1921 gcccctgtgc ctcttccatc ttcgccaacc ggctctccag cttctgaagt cgtgcgttca
     1981 attcatcgag ccgcggcttg acgggttcgg catctttgac ccgctcaact gattctaagc
     2041 gtcgctgcat cgcatcgaga cgctggtgat cctgatcctg agttttcgac acggtggcca
     2101 ggtgatcgcg caggtccacg aagtccgcgt gtctggcaca gccggtcgcg acgagcccga
     2161 tcacgacgag aacaagaaca gatctctgag cacacatgac gttctgcctc acagttatcg
     2221 agactctttt gtcatctgag tgagtttatc ggaggctttc ccagcttctg gactcttcgg
     2281 ataaagggtc acaacttgct tgaatgctga agacgctcgc ttcttgtctt tcagcgccaa
     2341 ataggcatac ccctttttga gcaacgcggc cggaactttc tcgctcttcg gataatcgag
     2401 ttccacccgg tcgtaggaac taatcgcttc cttatagtct ttttgaccgt actgcgcttc
     2461 tccgagccaa tagcgcgcgt tgggggccaa gtcggaattc ggatagctgg ttaagaatgc
     2521 ggcaaacccc cgctcagcgc caacaagatt gccgtccttg aagaggctta acagtcgttc
     2581 atactcagtt ttatcgggac gagcggcctc gggactgact tgaggctttg cctcatcgtc
     2641 gggctcatcc ggagccacaa cgacttgagc cgatcgcacc ggttctgcaa ttgcctgagg
     2701 ctgcgtctcc gcctgttccg gttcgcccgg cggttcggat ttttgcggtt ctgcctgcgg
     2761 ggcatacgcc gaccgatgtg ccggcttgga cggagactgg cgcgtcgcac ctttgcctcc
     2821 cgcacgcgca tgagcgtcct gcgcgacttg atcgaccgat ttcgccatca gctccagacg
     2881 acggtcctgc tcatcgatgc gcgcggccag cttttgagtg actgcagcca cgctcgtcac
     2941 actcttgttg acatcgccca agtgcccgga caccgatttg ttcatctcat tgacatacgc
     3001 cgtcgacgct ttcgagtctg cctccagctt gttgacgaag gtcttcgtcg actgctcgtc
     3061 ctgtgcgacc cgctcgctaa gccccgacag cgattcccga aacgcagtca atgattgatt
     3121 gaactgcgtg aacttcgcca ccagctgttc cgtccgattc tggttctcaa gagttgcctt
     3181 gtgttgctcc tcgaggcggg catcgatatt cttcgtgagc gcactattcg tcttctggac
     3241 ggcctcgata atatctttgc gcagaagctc catttgcttg ttcacatcgt cgagccgcgc
     3301 attgacctcc atgcggagtc gatcacgatc ttctttgttc ttgagatcct gggtttcgag
     3361 actcttctgg atccaggcat atcgggtggt cgt
//
LOCUS       DATDNW010000407         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_612195, whole genome shotgun sequence.
ACCESSION   DATDNW010000407 DATDNW010000000
VERSION     DATDNW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612195"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="serS"
                     /locus_tag="VI359_09670"
     CDS             <1..825
                     /gene="serS"
                     /locus_tag="VI359_09670"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY6974562.1"
                     /translation="AKIAGARFAVLTGAGARLERALINYMLDLHTTRHGYREVLPPFM
                     VNRSTMTGTGQLPKFEEDLFRLRDEDYFLIPTAEVPVTNLHRDEILNDDVLPLRYTAY
                     TPCFRREAGSYGKDTRGLIRLHQFNKVELVTFAKPDESYDELERLTGNAEAVLQGLGL
                     HYRVVTLCTGDMGFAAAKTYDIEVWLPSQNQYREISSCSNFEGFQARRANIRFRATGG
                     KKEAKTEYVHTLNGSGLAVGRTLVAILENYQQSDGSVLIPEVLRTYMGGLESIRKV"
     gene            900..989
                     /locus_tag="VI359_09675"
     tRNA            900..989
                     /locus_tag="VI359_09675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:934..936,aa:Ser,seq:tga)
     gene            1529..1729
                     /locus_tag="VI359_09680"
     CDS             1529..1729
                     /locus_tag="VI359_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974563.1"
                     /translation="MQKQIGKIGAIRRELRGKACPICGGHKYQLVLRGNVPPQSGTLF
                     ARCIQCQRPRGLDEDLGRILWM"
     gene            2272..2365
                     /locus_tag="VI359_09685"
     tRNA            2272..2365
                     /locus_tag="VI359_09685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2306..2308,aa:Ser,seq:gct)
     gene            2475..2550
                     /locus_tag="VI359_09690"
     tRNA            2475..2550
                     /locus_tag="VI359_09690"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2508..2510,aa:Phe,seq:gaa)
BASE COUNT          730 a          887 c          795 g          735 t
ORIGIN      
        1 gcgaagatcg ccggagcacg atttgccgtg ctgacaggag caggggctcg actcgaacgg
       61 gcgttgatca attatatgct tgaccttcac accactcgac atggatatcg ggaagtcctc
      121 ccgcccttta tggtcaaccg atcaaccatg actggaaccg gccagcttcc taagttcgag
      181 gaggatctgt ttcggcttcg cgatgaagac tatttcctca tcccaaccgc cgaggtcccg
      241 gtcaccaatc ttcatcgcga cgagattctc aatgacgacg tgctccctct gcgctataca
      301 gcctatacac cctgctttcg tcgagaagct ggatcgtatg gaaaggacac gagagggctg
      361 attcgcttac accaatttaa taaagtcgaa cttgtaacat tcgcgaagcc cgacgaatca
      421 tacgacgagc ttgagcggct caccggcaac gccgaagcgg tcttacaagg attgggactc
      481 cattatcgtg tcgtcacgct ctgtaccgga gacatgggct ttgccgccgc caagacatac
      541 gatattgaag tctggctgcc atcgcagaat cagtatcgcg aaatttcctc ctgcagcaac
      601 tttgaaggat ttcaggccag gcgggccaat atccgatttc gagcgacggg cgggaagaaa
      661 gaagccaaaa ctgaatatgt gcacacactc aacggatcag gccttgccgt gggccgaaca
      721 ctagtggcga tcttggaaaa ttaccagcag tctgacggaa gtgtgttgat ccccgaggtg
      781 ttgcgtactt atatgggtgg gctggaatcc ataaggaaag tctagaatcg tttgtaaggt
      841 gtctatatcc agtcggtggg agttatcata gcggctcctg gtcgggtcaa gcgtggtagg
      901 gaggggtgac ggagtggccg aacgtgccgg tcttgaaaac cggagatggg gtaacccatc
      961 cgcgagttca aatctcgccc cctccgccac actgagcgca cctcctttga tacccagata
     1021 ggataaacag gggaatcctg cgaactcttg ccctctcctt ctctcgtatc cttcgattct
     1081 cacttagggt agctacggag ttgcatacac accaacagtg cggttaaagt acatgagggt
     1141 gggggtgcct gctttggcca tgcccacgtc aaagcagcaa gaggtctctg ctccctcctg
     1201 gggagatctg cctccacccc cggcctcatt cggtcgctct tttcattcct tagtttctgt
     1261 gctctgccat cttgttatcg cttcaacgtt ctcctgctcg ggcctttcca aatactgcat
     1321 ctgttagatc actgttagac tcataagtaa ttgtaatatc tgtgatgtat tgaccatttg
     1381 ttatgagcaa cgggatctac ctttctccct cttttaggca gggcccacct acctctacgg
     1441 tattttcaga tttttcagtc tgctccaata tgagcctcag cgctgtacct caacctcagt
     1501 gcacgattca tgtggaggag gatccgcgat gcaaaaacag atcggcaaga tcggagcaat
     1561 tcggcgggaa ttgcgcggca aggcctgccc aatctgtgga ggccacaagt accaactggt
     1621 ccttcgaggc aatgtgcccc cccaatccgg caccttgttc gctcgctgca tacagtgcca
     1681 acgtcctcgt ggacttgatg aagatcttgg acgaattctt tggatgtaag gaaatcctcc
     1741 cacaaccccc acatggttaa tccatagacg aagcatgcga tactctctaa tccactccat
     1801 cgcaagcagc cgagcccgag caaaacagaa acgcactgcc cttcgcatga tgacactcag
     1861 cggagttatt cgacccagcc atgttgaact tcgatacgga acacagccgg caatctcttc
     1921 tgcctcgtgc cggcagcccg agacagccga gattccgctg ttcatagaac aggcccctgc
     1981 gcatgcctgg catcatgaga tcgcggccgg cacaaagcag atatggcgat gggtgcggat
     2041 cacttcgaga agcgtcctgc agcaagctcg aaaacccatc ggtcctcagt ggactatcga
     2101 ctagatcgcg tgaaagcgac tatcccggcg caaacccctc tctcgctgct ccgaatctct
     2161 tgtgccgctt gtacacgctg atgtaagatc agccctcact cgcaggcaat tcgatggcca
     2221 tcgtttcttc ctcgcagatg gccgggaacc ccgcgtacag agtgatcgtg tggagaggtg
     2281 gccgagtggc cgaaggcgac ggtttgctaa accgttgtag gggctaaaac ctctaccgag
     2341 ggttcaaatc cctccctctc cgccaccaaa ccaggtttgc ttgattcggc aaactcacgg
     2401 tagcctgaca gagcttgatt tttaccgttt gatccggtag acaagaccgc ctcacgtgac
     2461 caactcccgc ctgtgccgag gtagctcagt tggtagagca cagccctgaa aaggctggtg
     2521 tcgacagttc gattctgtcc ctcggcacca tttttccact acttacgcga ttctgaatta
     2581 gttctacgta ggatggtggc acctctagtg gcacctggag aggttttttc tggtcgttgg
     2641 cactaaatcc ttcgcgtaat ttctgctcgg tcaagatagc aagtcgagta accgcatcgc
     2701 gaagacgcga atcccaatca tggatgtaca agtcaccagt tccgggaagc cgatggccga
     2761 gcagtttttc gatcacgata taatctgttc cggcttcgag caaccaggtt gcgaaagtat
     2821 ggcgtaggtc atggaaatgg aggtcatgca cccctgcccg ttcaatcgtt tcgatccaac
     2881 gatgcttgaa ggagttcgca tccttccacc gcccaaagaa ccgaccaccg attcttggta
     2941 gctcacatcg caacgctgca cgagcaagat catttaatgg gatggctttc gcaactcgtt
     3001 tgatcgagct acgcccaggc gatggggcga gccataaccc atccccccgc tgaactaacc
     3061 actcgtcatg aatgtcgatc aatttacttt cgcgcattcc ggtctgaaga gccaaaatga
     3121 ttacacgcca gacctcctgc gaggcgg
//
LOCUS       DATDNW010000408        15135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_539918, whole genome shotgun sequence.
ACCESSION   DATDNW010000408 DATDNW010000000
VERSION     DATDNW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539918"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..770)
                     /locus_tag="VI359_09695"
     CDS             complement(216..770)
                     /locus_tag="VI359_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974564.1"
                     /translation="MRPTHRLLPTIAALLIATAPAFADTTVNVDPTRMWECPQADGTS
                     LYTNKELTGCKLMTLKQLSIVPSLEHMPTYVSPFPPAVPRYDGQPMATGSGNQVPDWA
                     RDWQASIASTGSVYAEVCSMYGEWLNLNQKTRGGFFFGSDPSYGGDLSGRNQRGPSYS
                     FYDNTRYRALAAIFGAGFVPVGCQ"
     gene            complement(940..1632)
                     /locus_tag="VI359_09700"
     CDS             complement(940..1632)
                     /locus_tag="VI359_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY6974565.1"
                     /translation="MIRAIIFDFNGVIADDETPHFHCFQQALEEHGLSITKEEYYGIY
                     LGMDERTCTVALLTARDGRCDRRLLHAIIERKAELFREHTASHQPPLFSGVVEFVKQA
                     GTTYRLAIASGGRREQITDALRATPIERDFAVIVSADECAVGKPDPAIYCYTLKLLNA
                     KEPRPPLITAAECLVVEDSMAGIRSAKTAGMRVLAIATTYPLEKLGEADLVLPGLNGV
                     TVGLIMDHFSSK"
     gene            1760..3778
                     /locus_tag="VI359_09705"
     CDS             1760..3778
                     /locus_tag="VI359_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY6974566.1"
                     /translation="MTDYEVLSNLLVIFTVSIAVVFLFHQFRLPSIAGFLVAGALIGP
                     NGLHLIADIGTVQVLAEIGVVLLLFTIGIEFSLVQLASLRRLLFLAAPIQVGGVLAVA
                     WLGASLVGLSWRQGIFWGFLLSLSSTAIVLKALADRGDSDSIHGRVTIGILIFQDLAV
                     VPMMLLTPILASQGEGASLSILVTLGKSVLLVGLVVAAAWYMVPKLLEHIVRSRSREL
                     FLLTIIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQAMAEVLPFRDSFNSLF
                     FVSIGILMDWRVVVEHPFVVAGLLPAILLVKFVTGAGAALIVEAPPRSAFMAGIALAQ
                     VGEFSFLLAQQGQESGLLRGDPYQIFLAVSVLSMIITPFLMQWSPDMARRIEAVQRLR
                     HWLPSRTTAHILQSESRQIRIKDHVIIVGYGLNGRNLARVLGETEIPFVALDLDGDTV
                     RRETRHGVPIYYGDGTNSNVLRHVKIEDAKVLVVAISDPFITRRAVQVAKGLNPKLHV
                     VVRTRYLRELEELHQLGADDVVPEEFETSIEIFALVLRTYNLPQDFVMRKAEQVRREG
                     YALLRRSEMPELAHHLRGGTLTDAEVETCRIDDDSPAQGKTLAELSIRPRTGVSVIAW
                     TRSGVTQSNPSEKTRLMAGDILVLLGSRAQIRRAMGLLMETGGESVVS"
     gene            complement(3799..4887)
                     /locus_tag="VI359_09710"
     CDS             complement(3799..4887)
                     /locus_tag="VI359_09710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY6974567.1"
                     /translation="MSTDLITEIKPSKSAPLLGFWYPGCLSSDLRPGTLRGVVLLGLP
                     IVVCRTSQGEVAALRDICPHRGMPLSFGRMEGERVECAYHGWQFDTTGRCRGIPALVE
                     DSPIQPEKIGVTSYPCRERDGYVWVFIPDPQQPTQTIPDVPPLPLPSDPYRKIHISTI
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRTKASIHEKAKTFEPLPRGFRMVPHEPSKNS
                     GPYKLLNFFYGGPLTTTIDFVLPNQRFEFVQCGSLFVSTRATVTPITEQQCRIDFTAA
                     WNCLGWMPFSKSLFRHFANIFMKQDREAMERQAVGLRYKPALMLIDDADTPAKWYYKL
                     KAAHLASTQTGQPFDHPLKERVTLRWKS"
     gene            5278..5808
                     /locus_tag="VI359_09715"
     CDS             5278..5808
                     /locus_tag="VI359_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY6974568.1"
                     /translation="MTTTQLDPAVALTDLQASKPDRVTIVLLSGDLDRAMAAFIIATG
                     AAAMGMQVTMFFTFWGLNTIRRKGATSAAKDWLRRMFGLLNKGGAENLPLSRFHFGGL
                     GTRMMQKVMKQNRMPGVPELMQTALDLGVRFIACTTTMGLMGITKDTLIEGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            5852..6097
                     /locus_tag="VI359_09720"
     CDS             5852..6097
                     /locus_tag="VI359_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY6974569.1"
                     /translation="MMHADVKLDTLGYFCPMPIIMTSKKIKELAVGQVLEVVSDDEGI
                     KKDMPAWCDTTGHQMVGLEEEQAKSGPIYKAFVKKTK"
     gene            6189..7862
                     /gene="ftcD"
                     /locus_tag="VI359_09725"
     CDS             6189..7862
                     /gene="ftcD"
                     /locus_tag="VI359_09725"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY6974570.1"
                     /translation="MNRIVECVPNFSEGRNLTTVHALMNAVTSVPGVWLLDHTKDHDH
                     HRAVLTIAGAPDAVAEAAFRAIRVATDLIDLRKHHGVHPRIGATDVVPFVPMQGTTMQ
                     DCIVLAKQVGQRVGRELDIPVFFYEQAATRQDHASLESVRRGGLDGLAFRMQSDPDWA
                     PDCGPSHLHPTAGAVVIGARPPLIAFNVNLQTTDLEVAKSIASTIRQSNGGVSHLKAI
                     GVSLASKKMVQVAMNLTDHTVTSLQAAFVAVSAQAEQRGVEVVGSEIVGLVPQAAMIQ
                     AASQSLRIDWFDPSQVLETRLALVLSGKRIEQTEPPMPAERDGPTRSIDSFAQSVAAA
                     TPVPAGGSVAAAVGALAASLGVMGAKLSQQPVAEHRLLAISQRLLHLVQADGEAYRQF
                     MRAMKLPAQDPARPTAVSTGLHMATEIPLEIAELSAEAGILLHACRKHARPKVQSDIT
                     VGMVIATAATQAGLHTVTENLKAQTNQRLKSALQIRITRVRRRLEELKGLCYTPPPGR
                     SVSRGKSGQASPEKVRRRHAWKLKSSITTSKKRSKLRRRSSRGKGSSAN"
     gene            7772..7966
                     /gene="rpsU"
                     /locus_tag="VI359_09730"
     CDS             7772..7966
                     /gene="rpsU"
                     /locus_tag="VI359_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY6974571.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKQREAQRRRQKWLAKRKPE"
     gene            8033..8692
                     /gene="nth"
                     /locus_tag="VI359_09735"
     CDS             8033..8692
                     /gene="nth"
                     /locus_tag="VI359_09735"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY6974572.1"
                     /translation="MRQQEIHAALRIVKREIRQWQEPVVGVVARESNRDPFRILISCL
                     LSLRTKDKTTSEASARLFALADTPRTLLKLPLRRIERAIFPVGFYRTKAKSIHQICRR
                     LLDVYGGVVPDSIDELVTLSGVGRKTANLVVTVGYGKPGICVDIHVHRICNRWGYVKT
                     KTPEDTEQALRRKLPTQYWITFNDLLVPYGQNLCQPVSPFCSHCKLTDYCDRVDVTKS
                     R"
     gene            complement(8779..9198)
                     /locus_tag="VI359_09740"
     CDS             complement(8779..9198)
                     /locus_tag="VI359_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974573.1"
                     /translation="MPSLPKFVLFAHNATYDKLHQVATLGLTAAAMGKEVIVVLLFWT
                     IKKLAEGKVDVIDFPPEYARSAEEVTRLLKEKKVPKISEMFQEAKLVGKFRLIACSAG
                     LEYMGVDSQAVEKNVDEVMGLPAIIRLTADAETKLFI"
     gene            complement(9202..9540)
                     /locus_tag="VI359_09745"
     CDS             complement(9202..9540)
                     /locus_tag="VI359_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974574.1"
                     /translation="MSISSPVIRLVSAVLWIALLATSPVLAGADELEIAVTKKGLGKE
                     VLITQGTKEWYMLVEVTPENTVVVRQEKEGETYLVDESETHDRPLAPGEVDQAITDYV
                     NSVKTRAQKK"
     gene            9643..10128
                     /locus_tag="VI359_09750"
     CDS             9643..10128
                     /locus_tag="VI359_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974575.1"
                     /translation="MMDGLLEKLQAIIPVKVLVGLTVASVVGFIGTLIAIPLILVRLP
                     PDYFDTRVPRHWMKDHHPVLRVIGLVVKNAVGVVFLLAGFAMLFLPGQGILTMLIGVS
                     LMDFPGKQRLEAKLIGHPTVLSAINAMRNKFGKPPLIMAPNPSRDRTQLSGREKSGAD
                     C"
     gene            10154..12808
                     /gene="polA"
                     /locus_tag="VI359_09755"
     CDS             10154..12808
                     /gene="polA"
                     /locus_tag="VI359_09755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY6974576.1"
                     /translation="MSASSKLKTLYLIDGSAYIYRAFFALPALNNSKGLQTNAVYGFT
                     TMLLKILRERQPDGLVVAFDEKGPTLRHQEFKEYKAQRPPMPEGMSAQIPYIHRIVEA
                     LNIPAVRQAGYEADDLIGTLARKAEQAGYDVVIVTGDKDMFQLVTPHVRIYDPVKDKW
                     SGEAECRERFGVEPARVVEIMGLMGDSTDNIPGVKGIGEKTAMKLIAEFGTIDELLRR
                     VDDVTPARTKSLLMEQADNARLSRKLATIDVDSPVEFDAAALHVKPPHAEQLTNLLRE
                     LEFTTLLKSFQSASQPAERQDMKTELIQDEPSAQRFMQGLPKDGALGLYCLCSGGSGI
                     QAAVQGIALSAAGKTAFIPLDVHTFMRPITTLLHETTRTKVVQDLKATLLAFHRIGLT
                     LAGPYLDTMVADYLLNPNRRDHQLDTIAMEVLGHRLGAGHGEHSTPQSLFEEDRGSRE
                     EAAEAASVLAKLAPALTERLSEQGSLKLFTDVEMPLVPVLAEIERNGFLLDVESLHSL
                     SKELERELDRIMETIAGLAGGEFNINSPKQLATVLFEKLGLKPLRKTKTGYSTDEDTL
                     MQLATQHDLPAQILNYRSLSKLKSTYVDALPELVNPETKRLHTSLNQTVAATGRLSST
                     EPNLQNIPVKGDYGLRIREAFIAPRGHTLLCADYSQIEPRILAHLSQDARLIGVFEKG
                     EDIHMATAMEIFGVPSSQVTKDMRRAAKTVVFGIVYGISPFGLSQNIGVSQTEAKKYI
                     DTFFERFAAVRALMDRNIAEGKTKGYTTTILGRRRPIPELQSGDPVQRGVGERMAVNS
                     PIQGSAADLIKVSMINVNQRLHHELPHTKMILQVHDELIFEVPEKELDEAKRLVKNEM
                     EATGKKLGLSVPLKVDLGVGKNWRVAHP"
     gene            12832..13089
                     /locus_tag="VI359_09760"
     CDS             12832..13089
                     /locus_tag="VI359_09760"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEY6974577.1"
                     /translation="MQNARLSLAWLIAGVSLFALGLLLVTGQPFSAKAQEPKRFQYKI
                     VEVIRDTQNMQTILNEQGANGWELVTVSMGDMTEPRLIFKK"
     gene            13135..13443
                     /locus_tag="VI359_09765"
     CDS             13135..13443
                     /locus_tag="VI359_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974578.1"
                     /translation="MPREASLFESADGSVLKGYRLLQRGGANIPPMWIQRASESRCRL
                     HKDVTQALRRKSKAGQRTLKEWEKRYNKECFYYGLRVLLELGRKGKTRMTKAPRSYVL
                     "
     gene            13540..14421
                     /locus_tag="VI359_09770"
     CDS             13540..14421
                     /locus_tag="VI359_09770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY6974579.1"
                     /translation="MKDNGQHRDDLPGSLQSAEVTRRRFMMTTLAVGFALAVRPVSAE
                     TIVTDAAGLRAGEVKIPTSDGDIPAYRAMPSQGRAFPVVLVVQEIFGVHEHIKDVCRR
                     FARLGYLAVAPELYARQGDVSKLSDIDEVRKVVAKVSDAQVMADLDAAVAWITASGDG
                     NIEKLGITGFCWGGRIVWLYAEHSSQLKAGVAWYGRLVGKPSELQPKHPIDVAPSLKA
                     PVLGLYGGNDQGIPLETVEQMQTVLKAANSPSRIIVYPDTPHGFHADYRPSYRKEQAE
                     DGWNRLQTWFKQHGAAS"
     gene            complement(14424..>15135)
                     /locus_tag="VI359_09775"
     CDS             complement(14424..>15135)
                     /locus_tag="VI359_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974580.1"
                     /translation="ESRKLTTGDRILVQTHGQGGLVTALVSNLLCPSAIMGRPKLFAL
                     LLSYAQQAGLADVIASLTKLEPLLASGSLLNGVVLDVVTFGTPVRYGWDPSGIGKLLH
                     IVNHRNLRTDGKTWLSKMELPQVTMEMPIAWGGDYVQELAVAGSDAMPTTEVAKAANK
                     AVWELVEPYDGFERWLECARRAVRFPTEGRCLLVDYKDSTGSTNPRDHYYGHAAYTRL
                     HAMLFNTTEIVRILYSIA"
BASE COUNT         3203 a         4258 c         4519 g         3155 t
ORIGIN      
        1 ggagaagaag ctgatgcccc tccaacccgg cgatgtccct gccacctatg ccgatatcga
       61 cgatctcatg cgagatgtgc gatttgcacc gtcgacaccg atcgaggaag gaatccctcg
      121 cttcgtgaag tggtatcgcg agttctacaa aaaataaagg gcgccgggct cttcatcagg
      181 cccgacgccc catctcggaa actctgatcg tctgcttact gacagccaac cggcacaaac
      241 cccgctccaa aaattgccgc tagagcgcgg tagcgcgtat tgtcatagaa cgagtagctc
      301 ggaccccgct gatttctccc cgagagatct cccccatacg acggatcaga tccgaagaag
      361 aacccgcctc gagtcttttg attcaaattg agccactctc catacatgga acagacctca
      421 gcatagaccg atccagtaga ggcgatgctt gcctgccagt ctctagccca atccggcact
      481 tggttgccgc tgccagtggc catgggttgt ccgtcgtagc gcggaaccgc aggaggaaag
      541 ggcgaaacat acgttggcat gtgttcgagc gacggcacga tcgacaactg cttcagcgtc
      601 atcaacttgc acccggtcaa ctctttgttc gtgtagagtg aggttccgtc agcctgaggg
      661 cattcccaca ttctggttgg gtcgacgttc actgtcgtat cggcaaacgc tggtgccgtt
      721 gcaatcaaga gtgcagcgat ggttggcagg agtcggtggg ttggcctcat aaaacacctc
      781 acacgaaatt tgaggatgga taagcgtctc atcgtggcgt cgccctttct ctatgctact
      841 aactaatggt gctgacaagc tatgccatca agcacaaata cttccattct tcttttgggg
      901 gggctatgtg gggaggcggc tgaggaatgg ctccagcagc tactttgacg agaagtgatc
      961 catgatcaga cccacggtga ctccgttcag gccagggagg acaaggtcgg cttcgcccag
     1021 cttttcaagg ggataggtgg tcgcgattgc aaggacccgc atgccggctg tctttgccga
     1081 gcggatccca gccatcgagt cttccacgac caggcactcg gctgctgtga tgagaggggg
     1141 gcggggttct ttcgcgttga gcagtttgag cgtgtagcaa tagatcgccg ggtccggctt
     1201 gccgaccgcg cattcatccg ccgagacaat gacggcgaaa tcccgttcga tcggtgtagc
     1261 gcgcagcgca tccgtaatct gttctcggcg accgcctgag gcaatggcca gtcgatacgt
     1321 cgtcccggct tgtttgacga attcgaccac gccggaaaac aacggtggtt ggtgcgaggc
     1381 cgtgtgctcc ctaaagagtt ccgcctttcg ttcgataata gcgtgaagca atcgtcggtc
     1441 gcagcgacca tcccgcgcag tgagcagggc gaccgtacag gtgcgctcat ccatccccag
     1501 atagattccg taatactcct ccttcgtgat cgaaagaccg tgctcctcca gcgcttgttg
     1561 aaagcagtgg aagtgcggcg tctcgtcgtc ggcaattacg ccgttgaagt cgaagatgat
     1621 ggctcgaatc atactcgtcc tgaggagctg acgggcttca ctctactcaa tggtccggtg
     1681 cgggacaagc agcttggcct tgttcgattc tctctgctgg ggtattctcc gcttcagctt
     1741 cttccgttcg agtcctgcta tgactgatta cgaagtcctc agcaatttgc tcgtcatctt
     1801 tacggtttcg atcgcggtgg tgtttctctt ccaccagttt cgtctcccgt cgatcgcggg
     1861 atttcttgtc gccggcgccc tgatcggccc gaacggcctg catctcatcg ccgatatcgg
     1921 cacggtgcag gtcctggcag aaatcggcgt cgtgttgttg ctcttcacca tcgggatcga
     1981 gttttcactc gtccaactgg cctcgttgcg gcgcctccta ttccttgccg caccgatcca
     2041 ggtcggtggg gttctggccg tggcctggct cggggcatcc ttggtcggtc tctcgtggcg
     2101 ccaagggatt ttctgggggt ttttgctctc gttgagcagt actgccattg tcttgaaagc
     2161 actggccgac cgcggcgaca gcgactcgat tcatggccga gtcaccatcg ggattctcat
     2221 ctttcaagac ctcgcggtag tccccatgat gctcctgaca ccgattctcg ccagtcaggg
     2281 ggaaggcgca agcctttcca tcctggtcac gctcggcaag tccgtccttc tcgtcggtct
     2341 cgtggttgca gcggcgtggt acatggtgcc aaagctgttg gagcacattg tccggagtcg
     2401 cagccgtgag ctgtttctct tgacgatcat cgtgctctgt cttggcatcg cctggctcac
     2461 gtcattgggc ggcctttctc tggcgctggg ggcgttcatt gcgggtctcg tcatttcgga
     2521 atcggaatac agtcaccagg ccatggctga agtgctgccg ttccgcgaca gtttcaacag
     2581 tctgtttttc gtatcgatcg gcatcctcat ggactggcgt gtggtggtcg agcatccctt
     2641 cgtggtcgcc ggcctgcttc ccgccatctt gctggtgaag ttcgtcacag gggctggtgc
     2701 ggcgttgatc gtggaggccc cgccccgttc cgccttcatg gcggggattg ccctggccca
     2761 agtcggggaa ttcagtttcc tgctggcgca gcaagggcag gaaagcggac tcttgcgggg
     2821 cgatccctat cagatcttcc tggcggtgtc ggttctgtcg atgatcatca cacccttcct
     2881 catgcagtgg tcgccagaca tggctcgtcg cattgaagcg gtccaacgat tgcggcattg
     2941 gctgccgagc cggacaaccg cccatattct gcagagtgaa agcagacaga ttcgcatcaa
     3001 ggatcacgtc atcattgtcg gctatgggtt gaatgggcga aacctcgccc gggttttggg
     3061 ggaaacggag atcccgttcg tggcgctgga ccttgacggc gatacggtcc gtcgggaaac
     3121 acgccatgga gttccgatct actatggtga tggcaccaat tcgaatgtct tgcgacacgt
     3181 gaagatcgaa gacgcgaagg tcttggtcgt ggcgatttca gatcccttca tcaccagacg
     3241 ggcggtgcaa gtcgcgaaag ggctgaatcc caagctgcac gtcgtcgtgc ggacccggta
     3301 tttgcgcgag ttggaagagt tgcatcaatt gggcgcggac gatgtcgtgc cggaggaatt
     3361 cgaaacgtcg atcgaaattt tcgcgttggt gctgcgcacg tacaatctgc cacaggactt
     3421 cgtgatgcga aaggccgaac aggtccggcg ggaaggttac gcgttgctcc gtcgcagcga
     3481 aatgccggaa cttgcacatc atttacgggg tggaacccta accgacgcgg aagtcgagac
     3541 ctgccggatc gacgacgatt cgccggcgca ggggaaaacc ttggctgagt tgtcgattcg
     3601 tcctcgaacc ggcgtgtccg tcatcgcgtg gacgcgcagc ggcgtcaccc aatcgaaccc
     3661 gtcggagaaa actcggctga tggccgggga tattttggtt ttgctgggat cacgggccca
     3721 aatcaggcgg gcgatgggat tgttaatgga gaccggaggc gagtcggtcg tttcgtaaga
     3781 cccgtcgaat tctgtgggtc aactcttcca gcgcagcgtg actcgttcct tgaggggatg
     3841 atcaaagggc tgaccggttt gagttgaggc cagatgggcg gctttaagtt tgtaatacca
     3901 cttcgccggc gtgtctgcat cgtcgatcaa catcaaggcc ggcttgtacc gtaatcccac
     3961 tgcttggcgt tccatcgcct ctcgatcctg cttcatgaaa atgttggcaa agtgacggaa
     4021 cagcgacttg gaaaagggca tccagcccag gcaattccag gccgcggtaa aatcgatgcg
     4081 acattgctgc tcggtgatcg gtgtgacggt cgcacgcgtg gagacaaaga gtgagccgca
     4141 ctggacgaat tcgaatcgct gattgggcag cacgaagtcg atggttgtcg tcaagggacc
     4201 gccataaaag aagttcaaca gtttgtaggg gccgctgttc ttcgaaggct catgcggcac
     4261 catgcgaaag ccgcggggca acggttcgaa ggtcttggct ttttcgtgga tactcgcctt
     4321 cgtacgccac cacgaactct gatggacaaa agggccgtgg gccggatcca tcagaccaac
     4381 gatgccgtcg tcgatcgtgc agtccaggat cgttgaaatg tggatcttcc ggtaggggtc
     4441 agacggcagc ggaaggggcg ggacatccgg aatggtctgg gtgggctgct gggggtcggg
     4501 gataaagacc cacacatagc cgtcccgttc acggcacggg tagctggtga ccccgatctt
     4561 ttcaggctga atcggtgaat cctcgaccag cgccggaatg ccgcggcatc gaccggtcgt
     4621 atcgaactgc cagccgtggt aggcacattc gacgcgctcg ccctccattc ttccaaagga
     4681 aagtggcatg ccgcggtgcg ggcaaatgtc gcgcagggcc gcaacctcgc cttgggaggt
     4741 ccgacacact acgatgggga gccctaggag cacgacacct ctcagagttc cggggcggag
     4801 gtctgaactc agacagccgg ggtaccagaa tccgagcagc ggcgcgctct tggagggttt
     4861 gatttcggta atgaggtcgg tgctcatcga caggggagga tgtgcgcgaa aggaccagcc
     4921 tagactgcag aagcagcttg gtcgtgaata taacgactag gtttttgaac gtcaagcgag
     4981 caccgtgtcg atttttgcct cccttcttgc tgcaaggcaa cctgctcggt ctcctgtgca
     5041 cgcgtccaac gagggccttc actcttcctg ccttcctgct cagggagttg ccagactgtc
     5101 cttcactgcg cgcatcgagc gacgaccgtt ttaccgtggg ggctctgcga gcaagaagga
     5161 tggtctggcg gctccctgag cttttcccct ttcttgacaa gaaaaagaag cggagactat
     5221 agtcaaaaaa tctcgaaaca ctcggattga tgcgcatcta agcgaagtga gggcactatg
     5281 acgaccacac aactggatcc ggcagtcgca ttgaccgatc tccaggcttc caaaccggac
     5341 cgagtcacga tcgtgctgct cagcggggat ttggatcgtg cgatggcggc cttcatcatc
     5401 gcgaccggag ctgccgcgat gggaatgcag gtcaccatgt tcttcacgtt ttggggattg
     5461 aacaccatcc gccggaaggg cgcgacgagc gcagcgaaag actggctccg gaggatgttc
     5521 ggcctgctca acaaaggcgg cgccgagaac ctgccgctct cgcgattcca ctttgggggg
     5581 ctcggcacca ggatgatgca aaaggtcatg aagcagaatc gcatgcccgg tgtaccggag
     5641 ctcatgcaga ccgctctgga tctcggcgtg cgttttatcg cctgcacgac gacgatgggg
     5701 ttgatgggaa tcacgaagga cacgctgatc gagggaatcg atcaattcgc tggcgtgacc
     5761 acctatttgg ccgaagcgaa acagggcagc gtcaatctgt ttatatagct gatggctctt
     5821 cgctgtcagc gttcagctag gggaattgtt catgatgcac gcagacgtga aactcgatac
     5881 gcttgggtat ttctgcccga tgccgattat catgacctcg aaaaaaatta aggaactcgc
     5941 cgtggggcag gtactcgagg tcgtatcaga cgacgaaggg attaagaaag acatgccggc
     6001 ctggtgcgac acgaccgggc atcaaatggt cgggctcgaa gaagagcagg ccaaatccgg
     6061 gccgatctat aaggcgtttg ttaaaaagac gaaataacga ccgcggtcag cgcggagcag
     6121 atgtagacta aggaagccgg aggggtggtg aaccgacctc cggctttgtc gttttgaacg
     6181 aggaccatgt gaatcggatc gtcgaatgtg tcccgaattt tagtgagggc cggaatctca
     6241 cgaccgtcca tgccctcatg aatgccgtga cgtcggtccc tggggtctgg ctcttggacc
     6301 atactaagga tcacgatcac catcgggccg tgctgacaat tgccggcgcc cccgacgccg
     6361 tggctgaagc ggcgtttcgc gcaatccgcg tggcgaccga cctcatcgat ctacgaaagc
     6421 atcatggcgt gcatccgcga atcggcgcca ccgatgtggt gccgtttgtg cccatgcagg
     6481 gaacgaccat gcaggactgc attgtattgg ccaagcaggt gggtcagcgg gtgggccgtg
     6541 agctcgacat cccggtgttt ttctatgagc aggccgcaac gcgccaggat catgcatcgt
     6601 tggagtcggt gcggcgcggt gggctcgatg gtttggcctt tcgaatgcag tccgatccag
     6661 actgggcacc ggattgtggc ccgtctcatc tgcatccgac agccggtgct gtcgtgatcg
     6721 gagcccgacc cccactcatt gccttcaacg tgaacttgca aacgaccgat ctcgaagtgg
     6781 cgaagtccat cgcgtcgacg atccgacaat cgaacggcgg cgtatctcat ctcaaagcca
     6841 tcggcgtctc actcgcgagc aagaagatgg tgcaggtggc catgaatctt accgatcaca
     6901 cggtcacatc cctacaagcc gcatttgtgg cggtcagcgc tcaggcggaa cagcgaggtg
     6961 tggaggtagt cggcagcgag atagtcggtc tggttcctca ggccgcgatg atccaggctg
     7021 cgagtcagtc gctccggatc gactggtttg atccgagcca agttttggaa accaggttgg
     7081 cgctcgtcct gtcgggcaag cgcatagagc aaacggagcc accgatgcca gctgagcgag
     7141 acggtccgac tcgatcgatt gattcgtttg cgcaatcggt agctgctgcg acgccggttc
     7201 cggctggtgg cagtgtggca gccgcagtcg gtgcattggc cgcgtcgctc ggcgttatgg
     7261 gggccaaact gagtcaacag cctgttgccg agcaccgatt gctcgcgatc agccaaagat
     7321 tgcttcatct tgtccaagca gacggcgaag cctatcggca attcatgcga gcgatgaaac
     7381 tgccggcaca ggacccagcc cgccctaccg ccgtgtccac cgggctgcac atggcgacgg
     7441 agattccact ggagatcgcg gagttgtcgg ccgaagccgg aatcttgctc catgcctgcc
     7501 ggaaacatgc aaggccaaaa gttcagtcgg atatcacggt ggggatggtc atcgccaccg
     7561 ccgcaactca agcgggactt catacggtga cggaaaactt gaaagcccag acaaatcagc
     7621 gccttaaatc cgcactccag attcgaatta cgcgggtgag acgccgcctt gaggaactca
     7681 aggggctatg ttacactccg ccccctggtc ggtcagttag ccgtggaaaa tctggacagg
     7741 catcgcctga gaaagtacgt agacgacacg catggaaatt aaagtcttca ataacaacgt
     7801 cgaaaaagcg ctcaaagttg cgaagaagaa gctcgcgggg gaagggctct tccgcgaact
     7861 gaagcggcgg cggtactacg agaagccgag cgtcaggaaa aaggccaagc agcgggaagc
     7921 gcaacggcgt cgtcaaaagt ggttagccaa gcgcaaaccc gagtgatcct cgcctagcag
     7981 gttcgttcga ttcatcaaga tctgctcgtc cgtccgttct ccgttctggc ccatgcgtca
     8041 gcaagaaatc catgccgctc tccggatcgt gaagcgcgag atccggcaat ggcaggagcc
     8101 ggtcgtcggc gtcgtcgctc gcgagtcgaa tcgcgacccc tttcgcattc ttatctcctg
     8161 tctcctgagt ttgcgaacca aagacaaaac gaccagtgag gccagcgccc ggctcttcgc
     8221 gttggcggac acaccgagga cgctactcaa actgcctctc cgccggatcg aacgggcgat
     8281 ctttccggtc gggttttatc ggacaaaagc caaatcgatc caccaaattt gccgtcgcct
     8341 cctggacgtg tatggcgggg tcgtgccgga ttccatcgac gagttggtca cactttcggg
     8401 agtcggtcgg aaaacggcaa atctggtggt gacggtcggc tatggcaagc ctggcatctg
     8461 cgtggacatt catgttcatc ggatctgcaa tcgctggggg tacgtgaaga ccaagactcc
     8521 ggaagacacc gagcaggccc tacgccgcaa attgccgaca caatactgga tcacctttaa
     8581 cgacttgctc gtgccctatg gccagaacct ttgccagccg gtctcaccct tctgcagcca
     8641 ctgtaagctg acggattatt gcgatcgggt cgacgtgacc aaatccaggt aggcgagagg
     8701 cgaggggcca gtggtcagga gtaacggaaa gaagacctcc tgtcctctag cctcttgccc
     8761 cgtgcctcta gccacgattc aaatgaacag cttcgtttcg gcgtccgcgg taaggcggat
     8821 gatggcgggg aggcccatca cttcatcgac gttcttttcg accgcttggc tgtcaacgcc
     8881 catatattcc aagccagcgc tgcaggcgat caggcgaaac ttcccgacga gtttggcttc
     8941 ctgaaacatc tcggaaattt tcgggacctt cttctctttc aacagccgcg taacttcctc
     9001 agccgatcgc gcgtactctg gtggaaagtc gatcacatcg accttgcctt ccgcgagttt
     9061 cttgatcgtc cagaacagca acacgacgat gacctctttg cccatcgcag ccgccgtcag
     9121 gccgagggtg gcgacttggt gcagtttgtc gtaggtcgcg ttgtgggcga agagcacgaa
     9181 ttttggcagt gacggcatcg gctacttctt ctgtgcgcgg gtcttcacgc tgtttacata
     9241 gtcggtgatg gcttggtcga cttcgcccgg cgcgagcggg cgatcgtgag tttcgctctc
     9301 gtcaaccaga taggtttcgc cttctttctc ctgacggacg acgaccgtat tctccggcgt
     9361 cacctcgacc agcatgtacc attctttggt accctgcgtg atcaggactt ccttgcccag
     9421 gccttttttg gtgaccgcaa tctcgagttc atcggctccg gcgagcacag ggctggtcgc
     9481 caggagcgcg atccacaaca ccgcgctcac cagacgaatg accggcgaag agatcgacat
     9541 ggttgagcga ttatagcctg tcgagaaatg cgtcgtaaac cgtggccgtc acactgtcat
     9601 tcgcttgagt gcccctaggt cgcatgttag gatgccgacc ccatgatgga cggccttctc
     9661 gaaaagctgc aagcgatcat tccggtcaag gtgctggtgg gactgacggt cgcgtccgtg
     9721 gttggattca tcggcaccct cattgccatc ccgctcattc tcgtccgtct tcctcctgat
     9781 tatttcgata cccgtgtgcc tcgtcactgg atgaaagatc accatcctgt cttgcgtgtc
     9841 atcgggctgg tcgtcaaaaa cgccgtcggt gtggtcttcc tcctggctgg ctttgccatg
     9901 ttattccttc cggggcaggg catcctgacc atgttgatcg gcgtctcgct catggatttt
     9961 cccgggaaac agcgactgga ggcaaaactg atcggccatc cgaccgtctt gagcgcgatc
    10021 aacgcgatgc ggaacaagtt cggcaagccg ccgctcatta tggcaccgaa tccgtcacgt
    10081 gatcgcacac agctgtcggg gcgtgaaaaa tcaggtgctg actgctgatg gctgaacgct
    10141 gtaggctgca tctatgtccg cttcgtcaaa actcaagacg ctctacctga tcgacggcag
    10201 cgcgtatatc taccgtgcct tttttgccct gccggccctg aacaattcca aaggtctcca
    10261 gaccaacgcc gtatacgggt tcaccacgat gctgctcaag attcttcgag agcggcaacc
    10321 ggacggtctc gtcgtcgcgt ttgatgaaaa aggaccgacc ctccggcacc aggaattcaa
    10381 ggagtataaa gcccagcggc cgccgatgcc ggaagggatg agcgcgcaga ttccgtatat
    10441 ccaccgcatc gtggaggcgc tgaatattcc cgccgtccgg caagcggggt acgaagcgga
    10501 tgatctcatc ggcacgctgg cacgcaaggc cgagcaggca ggctacgacg tcgtcatcgt
    10561 caccggcgac aaagacatgt ttcaactggt cacgccgcac gtacgtatct acgacccggt
    10621 gaaagacaaa tggtcgggcg aggctgaatg ccgtgagcga ttcggggtcg agcctgcccg
    10681 ggtcgtggaa atcatggggt taatgggcga cagcaccgac aacattcccg gcgtgaaggg
    10741 aatcggtgag aaaacggcga tgaagctgat cgcagagttc ggcacgatcg acgaactcct
    10801 gcgccgcgtg gacgacgtga cgccggctcg gacgaaatct ttgctgatgg aacaggcgga
    10861 caatgcacgg ctcagccgca agctcgcgac gatcgacgtc gatagccccg tagaattcga
    10921 tgcggccgcc ctccacgtca agccgccgca tgcagagcaa ctcacgaacc tcctccgcga
    10981 gttggaattc acgaccctcc tgaaatcgtt tcaatctgcc tctcaaccgg ccgagcggca
    11041 agacatgaag acggaactca ttcaggatga accgtccgcc cagcgattca tgcaaggctt
    11101 gccgaaagac ggggccttgg gcctgtactg tctgtgctct ggtggctccg gcatacaggc
    11161 agccgtgcaa ggcattgcgc tctcggccgc cgggaaaact gccttcattc cgctcgacgt
    11221 ccacacgttc atgcggccca tcaccacgct cctgcacgag acgacacgaa ccaaggtggt
    11281 gcaggacctg aaggccacgt tgctggcctt tcatcgaatc gggctcactc tggctggtcc
    11341 ctatctggac acgatggtgg cggactatct gctcaatccc aatcgccgcg accaccagct
    11401 cgacacgatt gcgatggagg tgctgggtca tcggttgggt gcaggacatg gggaacactc
    11461 cacaccgcaa tcgcttttcg aagaggatcg cggttcgcgc gaagaggcgg cggaagctgc
    11521 ctcggtcctg gccaagctcg ctccagctct gacagaacga ctctcggaac aagggagcct
    11581 gaagctcttt accgacgtag agatgccgtt ggtgcccgtc ctggccgaga tcgagcggaa
    11641 tggattcctg ctcgatgtgg agagtttgca cagcctgagc aaggaattgg aacgggaact
    11701 cgatcggatt atggaaacga tcgcgggatt ggctggcggt gagttcaaca tcaactcgcc
    11761 caagcagctc gcaaccgttc tattcgagaa gctggggctc aagccactgc gaaaaaccaa
    11821 gaccggctac tcgaccgatg aggacacgct catgcaattg gcaactcagc acgacctgcc
    11881 agcgcaaatt ttgaactacc ggagcctcag caaactcaag tccacctatg tggatgcgct
    11941 gccggagtta gtcaaccctg aaacaaagcg gctgcacacc tcgctcaatc aaaccgtggc
    12001 tgcgaccgga cggctctcat cgaccgagcc gaatctccag aacattcccg tgaaaggcga
    12061 ttatggcctg cgcatccgcg aagccttcat cgccccgagg ggccatacgc tgctctgcgc
    12121 ggactacagc cagattgagc cgcgcatcct cgcgcacctc tcgcaggatg cgcgcttgat
    12181 cggcgtcttt gagaagggtg aagacatcca tatggccacg gcgatggaga ttttcggtgt
    12241 tccgtcgagc caggtgacca aagacatgcg gcgtgcagcc aaaacggtgg tcttcggtat
    12301 cgtctatggc atcagcccgt tcgggctctc gcagaacatt ggcgtgtccc aaaccgaagc
    12361 caagaaatat atcgatacct tcttcgagcg ctttgcggcc gtccgcgcgc tcatggatcg
    12421 aaacattgcc gaaggaaaaa caaagggcta cacaacgacg attctcggcc gccgccggcc
    12481 gattccggaa ttacagagcg gcgatccggt gcagcgtggg gtcggtgagc ggatggcggt
    12541 caacagcccg attcagggct cggcggcgga tttgatcaag gtatcaatga tcaatgtgaa
    12601 tcagcggctc caccacgaac tgccgcacac aaagatgatc ctccaggtcc acgacgaatt
    12661 aatttttgaa gtgccggaga aggagttgga cgaagcgaag cggcttgtga agaatgaaat
    12721 ggaggccaca ggcaagaagc tcgggctctc ggtgccgctc aaagtcgatc tcggggtagg
    12781 caaaaactgg cgagtcgccc acccgtaacc gaaaacacag tgaggtgaga tatgcagaac
    12841 gcacgtcttt cactagcgtg gctgatcgca ggagtcagtc ttttcgcgtt ggggctcctc
    12901 cttgtgacgg ggcagccgtt cagtgcgaag gctcaagagc cgaagcggtt ccagtacaag
    12961 atcgtggagg tgattcgcga cacgcagaac atgcagacca tcttgaatga acagggcgcg
    13021 aacgggtggg agttagtgac ggtctcgatg ggggacatga cggagccgag actgatcttt
    13081 aaaaaatgag cgccagttgc caacccgtaa gaaaccgctc acggaggtga tgttatgcca
    13141 agggaggcat cattgtttga gagtgcagac gggagcgtct tgaaaggcta tcggctcctc
    13201 cagcgagggg gagcgaacat tccgccgatg tggatccagc gggcttccga gtcgcgctgc
    13261 cggctgcaca aggacgtgac gcaggcgctt cggcgaaaga gcaaagcagg gcagcgcact
    13321 ctcaaggaat gggaaaagcg atacaacaaa gaatgtttct attacggtct gcgcgtgctg
    13381 ctcgagttag gcagaaaggg taagacgaga atgaccaagg ctcctcgttc ttatgtccta
    13441 taaaacgccc gcacaatttc tatcgtattg aaccgcatta cattcaagca ggtggagacg
    13501 gcgcgaccga atgaatcctc catcacgttc gagaacacta tgaaggacaa cgggcagcat
    13561 cgtgacgacc tgccaggctc gcttcagtcg gctgaggtga cgcggcgcag gttcatgatg
    13621 acgacgctgg cggtcggctt tgctctggct gtgcgtcccg tttctgccga gacgatcgtg
    13681 acggatgcgg caggtttgag ggccggcgaa gtcaagattc cgacgagcga cggcgatatt
    13741 ccggcctacc gggctatgcc gtcacaagga cgcgcgtttc cggtcgttct ggtcgtccag
    13801 gaaatcttcg gcgtccacga gcacatcaaa gacgtatgtc gccgatttgc caggctgggt
    13861 tatctggctg tcgccccgga actctacgcg aggcagggcg atgtctcgaa gctgtccgac
    13921 atcgacgagg tccgtaaagt ggtggccaag gtgtcagatg ctcaggtcat ggcggatctc
    13981 gatgcggccg tggcgtggat cacagcctct ggggacggaa atatcgagaa gctcggcatc
    14041 acagggttct gctggggcgg gcggatcgtc tggctgtatg ccgagcacag tagccaactc
    14101 aaagcaggag tagcctggta tggacgactg gtcggcaaac cgagcgagct ccagcccaag
    14161 catccgatag acgttgcacc cagcctgaaa gcgccggtgc tcggcctcta tggcggaaac
    14221 gatcaaggaa tcccgctcga gacggttgag caaatgcaga cagtgctgaa ggcagcgaac
    14281 agtccgtccc ggatcatcgt ctatccggac acaccgcacg gtttccatgc cgactaccgg
    14341 ccgagctatc ggaaggagca ggcagaggat ggatggaatc ggctgcagac atggttcaag
    14401 cagcatggcg cggcatcgta agcctacgcg atgctgtaga gaatacggac gatctccgtc
    14461 gtattaaaca gcattgcgtg cagccgagtg taggctgcat ggccgtaata atgatcgcgc
    14521 gggttcgtgg agccggttga atccttgtaa tcgaccaaca gacaacgacc ttcagtgggg
    14581 aatcgcacgg cgcgcctggc gcattcgagc cagcgctcaa atccatcgta gggttcaacc
    14641 agttcccaca ccgctttatt tgccgccttg gcgacttcgg tcgtcggcat tgcgtcgctt
    14701 cccgccaccg ccagttcctg cacatagtcg ccgccccagg cgatcggcat ctccatggtg
    14761 acttgcggga gttccatctt cgagagccaa gtcttgccgt cggtacgcag gttacgatga
    14821 ttcacgatgt gcagcagttt cccgatcccc gatgggtccc acccgtaccg gaccggcgtg
    14881 ccgaacgtga ccacgtcgag gacaacgccg ttcagcagcg accctgacgc cagcaatggt
    14941 tcgagttttg tcagtgacgc gatcacatcc gcgagaccgg cttgctgggc ataagacaac
    15001 agaagcgcaa agagtttcgg acggcccatg atggcactgg gacagagcag attggatacg
    15061 agtgccgtaa ccaagccgcc ttggccgtga gtttggacga ggattcggtc cccggtcgtc
    15121 aatttccggc tctcg
//
LOCUS       DATDNW010000409         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_541363, whole genome shotgun sequence.
ACCESSION   DATDNW010000409 DATDNW010000000
VERSION     DATDNW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541363"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VI359_09780"
     CDS             complement(<1..946)
                     /locus_tag="VI359_09780"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974581.1"
                     /translation="MLRVITGRFHPHLESALVDHIRCAKADDPFAPIAVLVPSTSLLD
                     RVRRLLALEQHLSLLNVHFLTFHQLALRLADETRGQSHVASPRVVDDLVFEQLVRHIV
                     RSRLPSLAPLRHIGHSSGTWGALWSTIRDLKDAGIDPSAAVRGLGEGCFEKEDEAWLH
                     ALFSLHAAVKEVGRTLGVGTPDDLAESLIPLVPTLAFLTTLRQVFYYGFYDLTQVQLS
                     LFEAVSKTASTTLFFPLEEGASYGFSRRFFDRHILPCVTRPDALSRLGDARSPARTTD
                     VGPVTLSIRSVIGMDEELAVTCRTILDLVETNGYRFDDI"
     gene            complement(1133..1396)
                     /locus_tag="VI359_09785"
     CDS             complement(1133..1396)
                     /locus_tag="VI359_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY6974582.1"
                     /translation="MPNITLLVSPSCGACPSAKSLWKQLRVKYSFSYREVDITTQDGQ
                     DLANRHAVRAVPATIIDGRLTFVGVPSRESAEKALQLKMKPRE"
     gene            1984..2148
                     /locus_tag="VI359_09790"
     CDS             1984..2148
                     /locus_tag="VI359_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974583.1"
                     /translation="MGQTASYCSDCYNKVGRTQDADVKAAEGAGKTPMTTQGTCCKCN
                     KATIVVYYDK"
     gene            complement(2158..3258)
                     /locus_tag="VI359_09795"
     CDS             complement(2158..3258)
                     /locus_tag="VI359_09795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY6974584.1"
                     /translation="MGKIRHNGAVAKKLVGLADVVITAANRSKDPTLAIPIRALSNVS
                     FNPRKGLIEMGNKKQARSFFNVGMAKKFMQTILVADALTELQRADLTTSLREIYYRTK
                     HTIKNSHENTFDTQDESDPVIEDLEVSLEALREELHVRAENGGSVVGPLILGDDGDRV
                     DCAKLGKGGYSVPSIVEPDYVEIRRCTADFVLLVEKGTQWNRLSEDKFWRRYNCVLLT
                     GNGQPPRGVRRLARRLHEEYRLPVYVLVDNDPWGYYIYSVIKQGSINLAFESQRMAIP
                     KAKFVGLSSADPERYELPRNVGIKLNEKDITRAKELMNYQWFQKPAWQAEIKRMLASG
                     LKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            complement(3265..>3786)
                     /locus_tag="VI359_09800"
     CDS             complement(3265..>3786)
                     /locus_tag="VI359_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEY6974585.1"
                     /translation="RAAGEYRRRNIFELYIEEVAEACNRLKGGTLKTEKLKAQLQKIA
                     TSRTGGMKTDEALGKTGGGPEGLPDSIIVTPEGVEGEVAIAVDATNAIAQDAAPIADT
                     PAGKVLLVEKPIEKRATSTANVKYAKPAQQGKAAKGKAAQLVLFSGTSPASRSARAKT
                     KKAGKNHKARGKK"
BASE COUNT          792 a         1111 c         1042 g          841 t
ORIGIN      
        1 cgatgtcgtc gaaccgatac ccgttggttt ccaccagatc gaggatggtc cggcaggtca
       61 cggccaattc ttcgtccatg ccgatgacgc tgcgtatgga aagcgtgact ggcccgacgt
      121 cggtcgtgcg agcgggggaa cgcgcgtcgc ccagtctgct gagcgcgtcc ggtctggtta
      181 cacacggcag gatatgccga tcgaaaaagc gccgcgagaa tccgtaggag gctccctcct
      241 ccaatggaaa gaagagcgtc gtcgacgcag tcttactgac cgcttcgaag agcgacagct
      301 ggacctgtgt caggtcgtag aagccgtaat agaaaacctg gcgcagggtg gtcaggaaag
      361 ccaacgtggg cacgagcgga atcagcgact cggcgagatc atctggtgtg ccgaccccga
      421 gggtcctccc cacttctttc actgccgcgt gcaatgaaaa gagcgcgtgg agccacgcct
      481 cgtcctcctt ctcgaaacag ccttcgccga gaccacgcac tgctgcggat ggatcgatcc
      541 ctgcgtcttt caaatctcgg attgtggacc acagcgcccc ccacgttcct gaggaatgtc
      601 caatgtgccg aagtggagcc aggctgggga gccggctccg gacgatgtgc cggaccaatt
      661 gttcgaagac caggtcatcg acgactcgag gcgacgcaac gtgtgattga ccccgtgtct
      721 cgtcagccag tcggagagcc agttggtgaa aggtgagaaa gtgaacgttc aaaagcgaaa
      781 ggtgctgctc gagggccaga agacgccgaa cacggtcgag caacgaggtc gagggaacaa
      841 gaacagcaat cggcgcaaag ggatcgtcag ccttggcaca tcgtatgtgg tcaacgaggg
      901 ctgattcgag atgaggatga aagcggccgg tgatgacccg caacatagcc gggctatcta
      961 cccggtttct gggccgagaa gctctccatt acagtccaca caggcccagt cgccgtcgat
     1021 gaaggacatc ggatctccac aaatcgggca gtctggtggg ggtcccttat cgagaactgg
     1081 aaggggcggc agttcgaaat cgtcgtcatc gggaagcatg gggcggtctt tcctactcgc
     1141 gaggtttcat cttcaactgc aatgcctttt ctgcactctc gcggctcggc acaccgacga
     1201 aggtcagacg accatcaata attgtcgccg gcaccgctcg aaccgcatga cgattcgcca
     1261 gatcctgccc gtcctgagtc gtaatgtcga cttcgcgata ggaaaagctg tatttcaccc
     1321 gtaattgctt ccaaaggctc ttcgccgacg gacaggcgcc acaacttggt gacacgagca
     1381 atgtaatgtt cggcatcgaa ggtctccaac cgcttacgaa tcttaacacc gggaagaatg
     1441 agggggcaag gctggttcag tcacaaggaa tattcactgc aggatcacaa aacaacgggc
     1501 caatggtgga gggacgcata tacctggatg agggatcctg ggctcccatc ccacctgtga
     1561 gcggtggtcc acctggcaga cctggcagac ctcgtccggc tgatccaaaa gacttcccga
     1621 cgctagagct ttgcgccatg cctggcaacg ccggaggagg ctgggccggg gccgtggttg
     1681 atcctccagt gggtcccaca acatccaagg ctgccggctg aggattcggc tgcggttgat
     1741 tctgctggct aggcggtggc gtttgtgcgg cacagggagc gatgaacaag tttgccaaga
     1801 ttccagctat cagaatcaat tgccacatat agctcttttg ccttttcacg cagctttccc
     1861 tcgatctcca cggacacaat cgaaccatgc gagacggtta cgcagtttcc acattctgcg
     1921 ctatactttc cctaatacca ttctcttcat ccgatgtcat gtcggtcacg ccaaggaggg
     1981 attatgggac agacagcctc gtattgtagc gattgttaca acaaagtcgg cagaacccag
     2041 gacgccgacg tcaaagccgc ggaaggagcc ggcaagactc ccatgacgac gcaaggcacc
     2101 tgctgcaaat gcaataaggc aaccatcgtc gtctactacg acaagtagga ccaggtttca
     2161 gtccagccaa tctttctctt tcagttttcg cggcagatac ttcttcgtga gatactgaaa
     2221 gtccttattg gccaatgcgt cgagttcata cttgagtccg ctcgcaagca tgcgtttgat
     2281 ttccgcctgc caggctggct tttggaacca ctgatagttc atgagttctt tggcgcgcgt
     2341 gatgtccttc tcattaagct tgatgccgac gttgcggggc aactcgtacc gttcaggatc
     2401 tgcactcgag agaccgacga atttcgcctt ggggatggcc atgcgctgac tctcgaacgc
     2461 caggttgatc gagccttgct tgatcactga ataaatgtaa tagccccagg gatcgttatc
     2521 cacgaggacg tacaccggca gccggtattc ttcgtgcagc cggcgagcca ggcgacgcac
     2581 gccgcgcggc ggttgaccgt tgcctgtcag caacacgcaa ttgtaccgcc gccagaattt
     2641 atcctccgag aggcggttcc attgcgtccc cttttccacg agcaacacaa aatcagccgt
     2701 gcagcgacgg atctccacat aatccggctc gacgatcgac ggcacggaat agccgccctt
     2761 tcccaatttg gcgcaatcaa cacggtcgcc atcgtctccg aggatcaagg gacctacgac
     2821 actgccgccg ttctcagccc ggacatggag ttcttcgcgt agagcctcca gtgatacttc
     2881 aagatcctcg atgaccgggt ctgactcatc ctgcgtatcg aaggtattct catgcgagtt
     2941 tttaatcgta tgcttggtcc gataataaat ctctctgagc gacgtcgtga ggtccgcccg
     3001 ctgcagctcc gtcaatgcat ccgcaactaa gatcgtttgc atgaacttct tcgccatgcc
     3061 cacgttgaag aaggaccgcg cctgcttctt gtttcccatt tcgatcaaac ccttgcgcgg
     3121 attgaacgag acgttcgaga gtgcccgaat gggaatggcc agcgtcgggt ctttcgagcg
     3181 gtttgccgca gtgatgacca catcggcgag accgaccagt ttcttcgcca ccgcgccatt
     3241 gtgtctgatc ttccccatgg tgtcctactt ctttcctctg gctttgtgat tcttgccggc
     3301 cttcttcgtt tttgccctgg cgctcctgct ggctggtgac gtccccgaaa agagcaccag
     3361 ttgtgccgcc ttgcccttcg cagcttttcc ttgctgggca ggctttgcat atttcacatt
     3421 cgctgtgctc gtagcccgct tctcaatcgg cttctccact agaaggactt tgccagcagg
     3481 cgtgtcagcg attggcgctg cgtcctgtgc aatggcgttg gtcgcgtcca cggcgatcgc
     3541 cacttcgcct tcaactccct ccggtgtaac gatgattgaa tccggcaagc cctccggccc
     3601 tccaccggtt ttcccgagcg cttcgtccgt tttcataccg ccagtccgcg aggtcgcaat
     3661 tttctgcaac tgcgccttca atttctccgt cttcagcgtc ccgcccttga gccgattgca
     3721 agcttcggcc acttcctcga tgtagagttc gaagatgttg cggcggcgat attcacctgc
     3781 cgcccg
//
LOCUS       DATDNW010000410        11022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_542138, whole genome shotgun sequence.
ACCESSION   DATDNW010000410 DATDNW010000000
VERSION     DATDNW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542138"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VI359_09805"
     CDS             <1..468
                     /locus_tag="VI359_09805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974586.1"
                     /translation="LLLPLVIPEVMMGVSLLLVFVMAGMPLGLTTVILGHTVFNLPVV
                     IVILRAKLRKLDPCLQEAAGDLGASPWQIFRLVTVPLLRPAILGAVLMAFTVSLDDFI
                     VTFFVAGPGATTLPLKVYSMIKSGVSPEINALSALMVLLSMSLVGVSLLLQRR"
     gene            483..1532
                     /locus_tag="VI359_09810"
     CDS             483..1532
                     /locus_tag="VI359_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY6974587.1"
                     /translation="MKLYKMSVLGLVLFSVALGNGCQRGSEPGNRTLHYFTWSDYVSP
                     ELLTEFERRHGVKVVIDTFSSNEELLAKLQSGATGYDVTVPSDFMVSIMITQGLLAEL
                     DAKAIPNVAFLEEHLQRLAFDPEHRYAVPYLWGTVGIGYDSAAVTPPPDSWAVLWDPR
                     YTGKISMLNDQREVFGAVLRSMGKSMNVTDPVTIEAAKQKLIAQKPLVKTYASEHYDQ
                     LLASGDVILAHGWGGPVARAMRERPSIKYVVPKEGGTIWADCLVVMKSSQKKELAMQF
                     INFLLDTRVAAQTSERLLFAASNREAQRMVSRQVRENPAVYPPADLIPRLEWMTDVGK
                     AMRIYDRAWTELKIH"
     gene            1671..2975
                     /locus_tag="VI359_09815"
     CDS             1671..2975
                     /locus_tag="VI359_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY6974588.1"
                     /translation="MDCHRQVRVFYILAFLTVIFVVPSYVAAGPADTVEQDSSKPLRL
                     SVNEALSLFLSQNLDVLVAKYGIEYAKGQQITARLFPNPVMSIGTLSAYTQGHTAGNS
                     GQLFMQAQQLFELAGKRGYRIESAGYGAQSAEAAFEDAVRQLSFTVKDTYYRIQLALR
                     RLALAEENRDRFSRILDINTIRFKKGYIAEVDLIRIRLQMVDFQSQVIGALQEAESAK
                     GDLRQLLRLSPKTSLELTTDMDFRRIDPDIAKLRVVALDARPDIRAKRFTYSQREADL
                     KLAKAYRIPDVTIGGGYAIQGAQGPDNQQQFALNMGLPLPLFNRNQGGIRQAEVATQT
                     AEADLNKTMNLVENEVEVAYRNLMQSRRLVEAYIGGVLEDARSTFTIVERAYERGGAT
                     ILDLLDAARTSRTIQQNYIEALFNYQHNLFQLESAVGQEIAS"
     gene            2972..4159
                     /locus_tag="VI359_09820"
     CDS             2972..4159
                     /locus_tag="VI359_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974589.1"
                     /translation="MNVEVKGLAKARGAAIFSLPLHLTLTLLVLAGCGRGEEPAHLNA
                     ATPPLGSSGAVKSDAQPRIETAVVEFSPSHQALTLAGKVAYGEDRYSRISSPLQGRVV
                     EVRVRLGDRVKAGAVLLVVDSPDIAQAYSEYMKEDSDLQYATRAYGLAKDLYEDKAMP
                     LKDLKQAENELVKARAEFRRAKERLLSLRVPAEELNKPLDKQQITSRFEMKSPLAGTV
                     VERSVTPGQSVGGDPSQVLFTVADLDMLQVVADLYERDLSLVKEGQFAKVTVEAYPGL
                     DFPATVATIGDVVDPTTRTIKLRAWVNNEAHKLKPEMFARLHIEIGDSAQILTIPREA
                     ILEADGKQFVYVMEAPDRYVKREVKVAGASGDQARIIDGLRSGERIVVKGAVLIKGQE
                     VKG"
     gene            4213..7287
                     /locus_tag="VI359_09825"
     CDS             4213..7287
                     /locus_tag="VI359_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6974590.1"
                     /translation="MIAKLVEFALVQRVSVCLGGIVLLFSGLYAFHILDVVAYPDPSP
                     PMVEMITQYPGWSSEEIERQITIPIETTLLGMPGLRDVRSLSIFGLSDIKFYFDFSTD
                     YFTDRQEVLNRIAMLNLPGNVQPALSPWWAIAEIYRYELVGDETSLTELKALQDWKLQ
                     REFKRVQGVIDVTAFGGTTKEYHVDINPGALLSYEVSLPQVINALTNSNANVGGNYLT
                     IGAQNYNIRGLGLINGLEDIENVMVTEKSGTPVFIKNLGKVSVGHRVRLGKVGINERD
                     DVVEGVVLLQRGYKAEPVLERVRHKVEELNTWKLPQGVTVKTFYDRTTLIHTTVETVL
                     DILISGIVLVFIILFVFLGHFRTAVIVALTVPMALLFTFSMMVLVGESANLISLGAID
                     FGIIVDSTLIMVESIFSHLAHRKGAQLTVPMHITRAAREVGKPIFFATTIIVVAFIPL
                     FTMTGVPGKIFAPMSLTYGFALTGALLMAFTLAPALSAFLLTGPIREEETWLVRVIRQ
                     RYMRALNWALHHPVPIIGIVVGLFAMALIAATRLGGEFMPALEEGNLWVRATMPVDIS
                     FEQADRLSADVRRIFRQFPEVESVVSQLGRPDDGTDPTSFFNAEFLVNLKPRSAWRLE
                     LHHDKEALIEDIERILHTIPGVTFNFSQVIQDNVQEAMSGVKGENSIKLIGPDLKTLE
                     AKAVEIEQVMQQVKGVKDLGIFRLLGQPNLLIEVDRQACARYGLLVSDVNAVVQAAIG
                     GQAITRVFEGERWFDLVVRFFPEYRQDVDAIGSILVTNSEGAKLPLKQLASITMQTGA
                     FIIYRENNERYIPIKFSVRGRDLESTVKDAQRRIREQVTLPARYRIEWHGEFDQLQDE
                     KQRLSTVVPMSLLIILVLIYLGVDSLRDAILVLSTVPFALIGGIFSLMITGTDFSISA
                     AVGFISLFGVAIQGGLILVVRIRDLLDQGHNLTDAIVQSAEARMRPVLMTTLAAAIGL
                     FPAAVATGIGAQSQQPLARVVVGGMLTSAVLILLVLPVLYALLHRGLARKEQ"
     gene            7287..10364
                     /locus_tag="VI359_09830"
     CDS             7287..10364
                     /locus_tag="VI359_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY6974591.1"
                     /translation="MIVRLVEFALVQRALMLILGAVLFFGGLYAFHILDVVAYPDPSP
                     PMVEVITQYPGWSAEEIERQVTIPIETALTGMPGLTALRSLSIFGLSDIKFYFAFGTN
                     YFKDRQEVLNRLAMMSFPETISPTISPWWAIAEIYRYEMVGDGVSLTDLKTIQDWQLH
                     REIKRVPGVVDVTAFGGTTKEYHVDVNPGALLSYGVTLPQVLSALTNSNANVGGNYLT
                     VGAQNFNIRGLGLINSLNDIENVMVAEKNGTPIFVRNLGKVSLGHRIRLGKVGIDDRD
                     DVVEGVVLLQRGYKAVAVLERLKEKVEELNAWKLPAGVTIKTFYDRTVLINTTIETVV
                     DILISGMVLVFLILFVFLGHLRAALIVAMTIPLSLLFTFAMMVLVGQSANLISLGAID
                     FGIIVDATLIMVESVFFHLAHHTGPHLTTPQQVIRAARQVGRPIFYSTAIIVVAFIPL
                     FTMTGVPGKIFAPMSITYGFALLGALLIAFTLAPLLCSILLAGPIREQDTAIVRVIRR
                     LYQSVLKWALSHALFVVGLGLILLIVAIGALQFVGGEFMPALEEGNLWVRATMPVDIA
                     FEQAARLATEIRGIFRQFPEVANTVSQLGRPDDGTDPTSFFNAEFLVTLKPQNQWRKE
                     IVSKDKLVEEIEAVLAKIPGITFNFSQMIQDNVQEAMSGVKGENSIKLFGPDLRILEG
                     TATKIERVMKEVPGVKDLGIFRLMGQPNLLIKVDREACARYGLLVSDVNAVIQAAVGG
                     QGVTRVFEGEQWFDLVVRFLPEYRQDVESIGNILVSTPEGAKIPLKQLALISTQAGAF
                     IIYRENNERYIPIKFSVRGRDLESTVTQAQEVLASRVQLPERYRIEWAGEYDQLKDEQ
                     KRLTVIVPLALLVILFLLYTAFDSVRNALLVLATVPFALIGGVLSLVVTGTAFSISSA
                     VGVISTLGVAILGGVLLISRIGDFRDTGIPLREAIIKGADVQMRPILMATLGAAIGLL
                     PAALATGIGSQAQKPLARVVVGGMLTAAVLILVVLPVLYELVHRREGMIDQKE"
     gene            10369..>11022
                     /locus_tag="VI359_09835"
     CDS             10369..>11022
                     /locus_tag="VI359_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6974592.1"
                     /translation="MCKIIMQEGRRRMLVGLIGGLLLAGIAPTWAAQDTSRWLVPVQM
                     PYEAPPKSPDVPDFSVPPNNKPLSPEELQRAEALLPLLEGKQEFWAMGEFVHLGEPVV
                     PVLVKGLTMPGPRIRYNTIETLSMLHATGAVPSLLTTAKEPNEIPRVREHALRVAVRL
                     DPSKTPEAIAVMAKDQNSSIRKTAAFESRYVREKAVIPILIGMIADDERFVGLTAVQS
                     "
BASE COUNT         2356 a         2837 c         3263 g         2566 t
ORIGIN      
        1 ttgttgctgc cgcttgtcat tcccgaagtg atgatgggag tgtctttgct gctggtgttt
       61 gtgatggccg ggatgccgct cggtctgacg acggtgatac ttgggcacac agtcttcaac
      121 ctccctgtcg tcatcgtgat cctacgagcc aagttacgaa agctcgatcc gtgtctgcag
      181 gaagcggcgg gcgacttggg agcgtcgccc tggcagattt ttcgcctggt gaccgttccg
      241 ctgttgcgac ccgccattct cggagccgtc ttgatggcct ttaccgtgtc actcgacgac
      301 tttatcgtga ccttctttgt cgcaggccct ggggcgacaa cacttccgtt gaaagtgtat
      361 tcgatgatca agtccggcgt gtcaccagaa attaatgctc tgtcggcctt gatggtgctg
      421 ctctcgatga gcctcgtagg cgtttcactg ctccttcaac ggcgatagcg gctggcgtgg
      481 tcatgaaact gtacaagatg agtgtcctgg gcctggtcct cttcagtgtt gcacttggaa
      541 atggatgcca gcgggggtct gagccgggta atcggacgtt gcattacttt acctggtcgg
      601 attacgtgag tcccgagctc ctcacagaat ttgagcggcg acatggcgtc aaggtcgtga
      661 tcgacacctt cagcagcaac gaggaactgc tggccaaatt gcaaagcggc gcgacagggt
      721 acgacgtcac cgtgccgtct gatttcatgg tatcgatcat gatcacgcaa gggttgttgg
      781 cagagttaga cgcgaaagcc attcccaatg tcgctttcct tgaggaacat ctccaacgtc
      841 tggccttcga tcccgagcat cgctatgccg tgccgtatct ctggggaacg gttgggatcg
      901 gatatgattc cgccgccgtg acaccaccgc cggacagctg ggccgttctc tgggatccgc
      961 gctatacggg caagatcagc atgttgaacg atcaacgtga agtctttggc gcggtgctcc
     1021 gatcaatggg taagtcgatg aatgtgacgg atccagtgac gatcgaggca gcgaagcaga
     1081 aactgattgc ccagaagccg cttgtgaaaa cctatgcgag cgagcactat gaccagctct
     1141 tggcctccgg agacgtcatt ctggcgcatg ggtggggagg cccggtcgca agggccatgc
     1201 gtgagcgacc atcaataaaa tatgtggtac caaaagaagg ggggacgatt tgggccgact
     1261 gcctcgtggt gatgaaatcg tcccagaaga aagaattggc aatgcaattt atcaacttct
     1321 tactcgacac gcgcgttgcc gcacagacat cggaacgact gctctttgcc gcctcgaatc
     1381 gtgaagctca acggatggtc agtcgtcagg tgcgcgagaa ccctgccgtc tatcctccgg
     1441 ccgatctgat acctcgcttg gagtggatga ccgacgtcgg gaaagcgatg agaatctatg
     1501 atcgggcgtg gacagaactc aagattcact aagttgtggt ttctcaagag gttcttccac
     1561 tctttcggcg ttccctgtat aatgcaaccg atctcaagat cggtggtata ggtgagacgt
     1621 gggggcacct ccaccactgc tatggtcctt tcgtggggca ttcggtgttt gtggattgtc
     1681 atcgacaggt tcgggtgttt tatattctcg ccttcctcac agtcattttc gtagtgccgt
     1741 catatgtcgc agctgggccg gcggatacgg ttgaacaaga ttcttcgaag cctctccgcc
     1801 tcagcgtcaa cgaagcactg agtctcttcc tgagtcagaa tctcgacgta ttggtagcga
     1861 agtatgggat cgagtatgcc aagggtcagc aaattacagc ccggctcttc cccaacccgg
     1921 ttatgtcgat cgggacgctc agcgcgtata cgcagggtca tacggcgggt aacagcggac
     1981 agttgttcat gcaggcgcaa cagctcttcg aattggccgg aaaacgaggg tatcgcatcg
     2041 aaagcgccgg ctatggagcg caatccgcgg aagcggcttt cgaagatgcc gtccggcagc
     2101 tgagctttac ggtcaaggat acgtattatc gtattcagct ggccctccga cgtctcgcgc
     2161 tggccgagga gaatcgagac cggttttcac gtatcctcga catcaatacg attcgtttta
     2221 agaagggcta cattgctgaa gtcgatttaa tccggattcg cctgcagatg gtggattttc
     2281 agtcacaggt cattggagcg ttgcaggaag cggaatcggc caagggagac ctgcggcaat
     2341 tattacggct ctctccgaaa acatcgttgg aactcacgac cgatatggat tttcgtcgaa
     2401 tcgatcccga tatcgccaag cttcgggtgg tggctctcga cgcccgtccc gatattcggg
     2461 cgaagcgatt tacatactct caacgtgagg cggatttgaa attggccaaa gcctatcgga
     2521 ttcccgatgt gacgatcggc ggaggctatg caatccaggg tgcgcagggg ccagacaatc
     2581 aacagcaatt tgcgctcaat atgggtctgc cgctcccttt gttcaatcgc aaccagggtg
     2641 gcatccgcca agccgaagtg gccactcaaa cggcggaggc cgacttgaac aaaaccatga
     2701 atttagttga gaatgaggtg gaagttgcgt accgcaatct catgcagagc aggaggcttg
     2761 tggaagccta tatcgggggt gtgctcgaag atgcacggtc cacatttacg attgttgagc
     2821 gggcgtatga gcggggtgga gcgaccatcc tggatcttct tgatgctgca cggacgtcac
     2881 ggacgattca gcaaaattat atcgaggccc tgttcaacta tcagcacaac ttgtttcagc
     2941 tagaaagcgc agtggggcag gagatcgctt catgaacgtt gaggttaaag gtctggcgaa
     3001 ggcgaggggt gctgccatat tttccttgcc cctgcaccta accttgactt tgttggtttt
     3061 agccggatgt ggccgcgggg aagagccggc tcatttgaat gctgcaactc cacccctggg
     3121 aagcagtggg gcggtcaaat ctgatgcgca gccgcgcatc gagacggcgg tggtcgaatt
     3181 tagtccctcg catcaggcgc tgacacttgc tgggaaggtt gcctatgggg aggaccgata
     3241 ttcccggatt tcctcgccac tgcaaggtcg tgtcgtcgaa gtgcgggtac gtttaggtga
     3301 cagggtaaaa gcaggagcgg ttctcctggt ggttgacagc cctgatatcg cccaagcgta
     3361 ctcggaatat atgaaggaag actccgattt gcagtatgcg acgagggcct atgggttggc
     3421 caaggatctt tacgaagaca aggcgatgcc gcttaaagat ctcaagcaag ccgagaacga
     3481 gttggtcaag gctcgggcag aattccgccg tgccaaagag cgattgctgt cgctgagagt
     3541 gcctgccgaa gaattgaata agcccctgga taagcaacag atcacatcac gatttgaaat
     3601 gaaaagcccc ttggccggca ccgtcgtcga gcgatcggtc acgccggggc aatcggttgg
     3661 tggtgatcct agccaggtgt tgttcaccgt agcggatctg gatatgctcc aagtcgtggc
     3721 ggatctctat gaacgtgacc tttccttggt gaaagagggc cagtttgcca aggtcaccgt
     3781 cgaagcctat cccggcctcg attttccggc cactgtcgcg accattggcg atgtggtcga
     3841 cccaacgacg agaacaatca agttgcgggc gtgggtcaat aacgaagccc acaagctcaa
     3901 gcctgaaatg tttgcccgcc tgcacatcga gataggggat tcagcccaaa tcctgaccat
     3961 tccacgtgag gcaatcctgg aagcagatgg aaagcaattc gtctacgtga tggaagctcc
     4021 ggaccggtac gtcaaacgag aagtcaaagt tgccggggcg tcgggagatc aggctcggat
     4081 tatcgacggg cttcgttcag gcgagagaat cgtggtcaag ggagctgtgc tcatcaaggg
     4141 acaggaagtc aagggataag acggctcact gccagcgcgt aacatgcagc ggttgaacaa
     4201 tcccctacac gtatgatcgc caagctggtc gagttcgcac ttgtccagcg agtgtccgtc
     4261 tgcctcggcg gtatcgtcct tcttttcagc ggcctgtacg cctttcatat cctcgacgtc
     4321 gtcgcgtatc ccgatccctc gccgccaatg gtcgagatga tcacccagta cccgggatgg
     4381 tcatcggaag aaatcgaacg acaaatcacg attcccattg aaacgacact tctcggcatg
     4441 ccgggcctca gagatgtccg ctccctctcc atttttggcc tgagcgacat caagttctac
     4501 ttcgacttct ccaccgatta cttcacggat cgacaggaag ttctgaatcg gattgcgatg
     4561 ctcaatttgc cggggaacgt acagccggcg ctgtcgccct ggtgggcgat cgcggagatc
     4621 taccggtatg agctggtcgg tgacgagacg tcgttgactg agctgaaagc actccaggat
     4681 tggaaacttc agcgcgaatt caagcgggtc caaggagtga tcgatgtcac ggccttcgga
     4741 ggaaccacca aggagtacca tgtcgacatc aatccggggg cgctgctcag ctatgaggtg
     4801 agtctcccgc aagtcatcaa tgcgttgacc aacagcaacg ccaacgtcgg cggcaactat
     4861 ctgacgatcg gagcgcagaa ttacaatatt cgcgggctgg ggctaatcaa tggcttggaa
     4921 gacatcgaaa atgtgatggt gacggagaaa agcggaacgc cggtgttcat caaaaacctc
     4981 ggcaaggtca gcgtcgggca tcgggtgcgg ctgggcaagg tgggtatcaa cgagcgcgac
     5041 gatgttgtgg aaggcgttgt gctgctccag cgaggctaca aagcggagcc ggtcctggaa
     5101 cgggtgagac acaaagtcga ggagctgaac acatggaagc tgcctcaagg agtcacagtc
     5161 aagacctttt acgatcgaac gacgttgatt catacgacgg tcgagacggt attggacatt
     5221 ctgatcagtg ggatcgtgct ggtgttcatt atcctgtttg tgtttctcgg gcattttcgg
     5281 acggctgtaa tcgtcgcgtt gacggtgccg atggcactcc tcttcacgtt cagcatgatg
     5341 gtcttggtcg gggaatcagc gaacctgatc tcgcttggcg caattgattt cggcatcatc
     5401 gtcgattcga cgctgatcat ggtggaaagc atcttttccc accttgctca tcggaaaggt
     5461 gcgcagctga cggttcccat gcacatcacc agagcagcga gggaagtcgg gaagccgatc
     5521 ttcttcgcga ccaccattat tgtcgtggcg ttcatccccc tcttcacgat gaccggggtg
     5581 ccgggcaaga tcttcgctcc gatgtcgttg acgtacggtt tcgcgctcac cggcgcgttg
     5641 ctgatggcct ttaccttggc tcctgccctc agtgcctttc tccttactgg ccccattcgt
     5701 gaagaagaaa cctggctggt gcgggtgatc cgacaacggt acatgcgcgc attaaactgg
     5761 gcgcttcatc atccagtgcc gattattggg atagtggtgg gattatttgc gatggccttg
     5821 attgccgcca ccaggctcgg tggcgagttc atgccggcgt tggaagaggg aaatctgtgg
     5881 gtgcgggcga cgatgccggt ggacatctcg tttgagcaag cggatcgtct gtcggcggac
     5941 gtcaggcgca tattccggca atttcctgaa gttgagagcg tagtgtctca attgggccgt
     6001 ccggatgatg gaaccgaccc tactagtttc ttcaatgccg agtttctcgt taatcttaag
     6061 ccccgctcgg catggcgtct cgagctacac cacgataagg aggcattgat cgaagatatc
     6121 gagcgcatac tgcatacgat tccaggggtc acgttcaatt tttcccaggt catccaagac
     6181 aacgtgcagg aggcgatgtc cggggtcaaa ggggaaaatt ccattaaact gatcgggccg
     6241 gatctcaaaa cactggaagc caaggcggtg gaaatcgagc aggtgatgca gcaggtgaag
     6301 ggggtcaaag acctggggat tttccggctt ctgggccagc ccaacttact catcgaggtg
     6361 gatcgacaag cctgcgcacg atacgggtta ttggtgtcgg atgtgaatgc ggtcgtccag
     6421 gctgcgatcg gaggacaggc gatcacgaga gtatttgaag gggagcgttg gtttgatctt
     6481 gtcgtcagat tctttcccga gtaccgccag gatgtggatg cgattggcag cattcttgtc
     6541 acaaattcgg aaggagcaaa acttccgctc aagcaactgg cgtcgatcac gatgcagacc
     6601 ggtgcgttca ttatctatcg tgagaataac gagcggtaca ttccgatcaa gttcagcgtg
     6661 cgtgggcgag atctggaaag cacggtaaaa gatgcccaac ggcgcattcg agagcaggtc
     6721 acgttgccgg cccgctaccg gatcgaatgg cacggcgagt tcgatcagct gcaagatgag
     6781 aaacaacgac tgtcgaccgt tgtacccatg agtctcctca tcattcttgt gctcatctat
     6841 ctcggcgtgg attcgcttcg cgatgcgata ctggtgttgt ccaccgttcc atttgccctg
     6901 atcggcggga tcttttcgct gatgatcacg ggaaccgact ttagcatctc tgccgccgtg
     6961 ggtttcattt ccctttttgg agtcgcgatc caaggggggc ttatccttgt cgtccgaatc
     7021 cgtgatttgt tggaccaggg gcacaatctg accgatgcca tcgtgcaaag tgctgaagcg
     7081 cggatgcggc cggtcctgat gacgacgctg gcagcagcaa tcggactgtt tcccgccgcg
     7141 gtggcgaccg ggatcggagc tcaatctcaa cagcccttgg cgcgggttgt cgtcggcggg
     7201 atgctcacgt cggctgtgct gattcttttg gttctcccag tcttatacgc gctcctgcat
     7261 cgaggcctcg ctcgcaagga acaataatga ttgtccgact cgttgaattt gcgctcgtcc
     7321 agcgtgcgct tatgttgatt ctcggagcgg tgctgttctt cggtggtctc tacgcctttc
     7381 atatcctcga cgttgtggcc tatcccgacc cctcgccacc gatggtggaa gtcatcactc
     7441 agtatccagg ttggtcggcc gaggaaatcg agcggcaggt gacgataccc atcgaaacag
     7501 ccctcaccgg catgccgggt ctgaccgcct tacgctcgct ctccatcttc gggttgagcg
     7561 atatcaaatt ctattttgcg ttcgggacga actacttcaa ggatcggcag gaagtgctga
     7621 accggctagc gatgatgtcg tttccagaaa ccatctcgcc gacgatctcg ccttggtggg
     7681 ccatcgcaga gatctatcgc tacgaaatgg tcggtgacgg cgtgtccctg acggacttaa
     7741 aaacgattca ggattggcaa ttgcatcgtg agatcaaacg agtgcccggt gttgttgacg
     7801 tcacggcctt cggcggcacg acgaaggagt atcacgtcga tgtcaatccc ggcgccctgc
     7861 tgagctacgg cgtcactctg ccgcaagtct tgagcgctct gacgaacagc aatgccaacg
     7921 tcggaggcaa ttacctcacg gttggagcgc agaatttcaa tatccgagga ctggggttga
     7981 tcaacagctt gaacgatatc gaaaacgtaa tggtggcgga gaaaaatggg actccgatct
     8041 ttgtacggaa tctcgggaaa gtcagtctgg gccatcggat acgtctaggt aaggtcggca
     8101 tcgacgaccg agacgacgtg gtcgaaggtg tggtcctgct gcaacgtggt tacaaggcgg
     8161 tagcggtctt ggagcgactc aaggagaagg tcgaggagct gaatgcgtgg aaactgccag
     8221 ctggtgtcac gatcaagaca ttctacgatc gaacagtatt gatcaacacc acgatcgaga
     8281 cggtcgtcga tattctgatc agcggtatgg ttctggtctt tctcatcctt ttcgtgttcc
     8341 ttgggcatct gcgtgccgcg ttgattgtgg ctatgacaat cccgctctcc ttgctcttta
     8401 cgttcgcgat gatggtgcta gtcgggcaat cggccaacct catctcgctc ggtgccatcg
     8461 atttcgggat catcgtcgac gccacgctca tcatggttga aagcgtgttt tttcatttgg
     8521 cccaccatac cgggccgcat ttgacgacac cgcaacaggt cattcgagcg gcccggcagg
     8581 tgggacgacc gatcttctat tctacggcta ttattgttgt ggccttcatt ccgctgttca
     8641 cgatgaccgg cgtcccagga aagatctttg ctccgatgtc catcacctat gggtttgcgt
     8701 tgctcggcgc gctcttgatc gcgttcacgc tcgcgccgtt gctgtgttcg attctgctcg
     8761 caggacctat tcgtgaacag gacacggcga tcgtacgagt cattcggcgg ctgtaccaat
     8821 ccgtcttgaa atgggcgctc agccatgcac tgttcgttgt cgggctggga ctgatactgt
     8881 tgattgtcgc cataggcgcg ttgcaatttg tgggaggtga gttcatgccg gcgttggaag
     8941 agggcaatct gtgggtgcgc gccacgatgc cggtcgatat cgcctttgag caagccgccc
     9001 ggttggccac tgaaatcagg ggaatctttc gacagtttcc cgaagtcgcg aacaccgtgt
     9061 ctcagttagg acggccggat gacgggaccg acccgacgag tttttttaat gcggagtttc
     9121 tggtcacgct caaacctcag aaccaatgga ggaaggaaat cgtatcaaag gacaagctcg
     9181 ttgaggaaat tgaggctgtc ttggcaaaga ttcccggcat cacgtttaat ttttcccaga
     9241 tgattcagga caacgtgcaa gaggcgatgt ccggtgtgaa aggcgaaaac tcgatcaagt
     9301 tgttcgggcc ggatctgaga attttggagg gtacagcgac aaaaattgag cgggtcatga
     9361 aagaagtccc gggggtgaag gatttgggca ttttccggct gatgggccag ccgaacctct
     9421 tgatcaaggt cgaccgggag gcctgcgcgc gctacggcct gctggtgtcg gatgtgaacg
     9481 ccgtgatcca ggccgcggtg ggcgggcaag gggtgacgcg cgtttttgag ggtgaacagt
     9541 ggttcgattt ggtggtgcgc ttcctccccg agtatcggca ggatgttgag agcatcggca
     9601 atatcttggt cagcacgccg gaaggagcaa aaattccgct caagcagctg gcgttaattt
     9661 cgacccaagc gggggcgttt attatctacc gggagaacaa tgagcggtat attccgatta
     9721 aattcagcgt ccgcgggcga gacttggaaa gtacggtgac acaggcccag gaggtgttgg
     9781 cgagccgcgt tcagctgccg gaacggtatc gaatcgaatg ggcgggggag tacgatcagc
     9841 tgaaagatga gcagaaacgg ctcaccgtca ttgtgccctt ggcgctatta gtgatcctct
     9901 ttcttctcta taccgctttt gattcggtgc gaaatgcttt gctggtgctc gcgaccgtac
     9961 cctttgctct catcgggggc gttctctcac tcgttgtgac gggaaccgca tttagcattt
    10021 cctccgccgt cggcgtcatc tcgaccttgg gcgtagcgat tctcggcggc gtcttgctga
    10081 tctcccgcat cggggatttt cgggataccg gtattccgtt gcgggaggcc attatcaagg
    10141 gagcggacgt tcagatgcgt ccgattctga tggccacgct cggtgcggcc atcggactcc
    10201 tacccgcggc gctcgccacc ggtatcggat ctcaagccca aaagcctctg gcgcgagtcg
    10261 tcgtcggcgg catgttgacc gcagcggtct tgattttggt ggtcctgccg gtgttgtatg
    10321 aactggtaca cagacgagaa ggtatgatcg atcagaagga atagtcatat gtgcaaaatc
    10381 atcatgcaag aagggagacg caggatgttg gttggtttaa ttgggggact tctcctagca
    10441 ggaattgcgc cgacatgggc cgcgcaagac acaagtcgct ggctcgtacc ggtccaaatg
    10501 ccatacgaag ccccgccgaa atctccggat gttcctgatt tctcggtccc tccaaacaac
    10561 aaaccactga gccccgagga gcttcagcgg gccgaagctc tcttaccgtt gctcgaagga
    10621 aagcaggagt tctgggcgat gggcgaattc gtccatctcg gcgagccggt cgtaccggtc
    10681 ttggtcaaag ggctgactat gccgggtcca cgcatccgct ataacacgat cgaaactctt
    10741 tcgatgctgc atgcgaccgg ggcggtcccc tccttgctca cgacggctaa agaacccaat
    10801 gagatcccaa gagtacggga acatgccttg cgggtagccg tgcggttgga cccgtcaaaa
    10861 actcctgagg cgattgccgt aatggccaag gatcaaaatt cttcgatccg gaagactgcg
    10921 gcattcgaat cgcgctatgt ccgcgaaaaa gccgtgatcc ctatcctgat cgggatgatt
    10981 gcggatgacg aacggtttgt gggactgacg gcggttcaat cc
//
LOCUS       DATDNW010000411         5779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_950001, whole genome shotgun sequence.
ACCESSION   DATDNW010000411 DATDNW010000000
VERSION     DATDNW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950001"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VI359_09840"
     CDS             complement(<1..828)
                     /locus_tag="VI359_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY6974593.1"
                     /translation="MNRDITPYVLKRSSDEAIPRRLSINYAAELNPQQHAAVTAGNGP
                     ALVIAGAGSGKTRTLVYRVAYLIDSGVDPASILLLTFTRKSAQEMLERAGALIGGRSE
                     RVCGGTFHSVANMLLRRFGRQIGLEPGFTILDRSDAEDLVALVRSQLGLNEKDKRFPR
                     KGTIAEIFSKAENTLRPIGEILLEEFSHFADHLDALERLQKGYQTAKRQKQLVDYDDL
                     LVLLRRLLMQDDTARQSIASLYRYILVDEYQDTNRLQGDLIRLLAATHQNVMVVGDDS
                     "
     gene            1016..1870
                     /locus_tag="VI359_09845"
     CDS             1016..1870
                     /locus_tag="VI359_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974594.1"
                     /translation="MAATKSPYSPQLRTIAELMLAVAGESVTMMKRNAPQQALKLKRQ
                     TEWKIYLEFLKVMFNLTDRLSVLHLPLKDQPEFMNDLEDAVTLQLKTVLEPAFASGGD
                     QTEIMLTISGAVAESRQIYEKYRFLITEDSPAKNDLLKDFGDRVADVMGVAGDGQVSS
                     AATLCVSAVIPALTSILAGDAPPTQDTLQALQNQSSSSTEKPASSTPKGPVGSEIKLV
                     SVMSSVVGEEVETRWGLHPRFRQDLKPDEVQQLTKLMNRVSKILGERYATVAFSDEWA
                     SWHKAGHA"
     gene            1931..4090
                     /gene="glgP"
                     /locus_tag="VI359_09850"
     CDS             1931..4090
                     /gene="glgP"
                     /locus_tag="VI359_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY6974595.1"
                     /translation="MDNPKQTESLPNGIPSGLTRLPELAQNLWWSWTPEARRLFEVID
                     PTVWVLCHHNPVKLLTEVKPERLTQLAEDPSYVRQYSAVLKLFDQYHQNGRSWLKSHH
                     AELAGTTIAYFSAEFGLHSSIPIYSGGLGILAGDHCKEASDLGVPLVGIGFMYPQGYF
                     RQRITAEGWQEAAYAPFNRDDSPIRPALTPSGTPCRITVEMGDRIVSAIVWKVLVGRV
                     TLYLIDTDVPENSPENRALSARLYGGDQEIRLCQEILLGIGGVRMLRTLGINPTVWHA
                     NEGHSAFLTLERVRELVQKGRSHAEASETVRQSTVFTTHTPVPAGHDVFPHHLMDRYF
                     NGYWEQIGLSRDEFLGLGDSPSSHQQGFNMTALAIRLSANVNGVSREHGRVTREMWQH
                     FWPGLAIDLVPIRSVTNGVHAPTWIAAEVSQLYGKYLGADWVDKCDDSAIWQRVLDIP
                     DHELWAVRQTMKRKLMSFIRERARSGWMQGHLQATQVLSRGTLLDPEALTIGFARRFA
                     TYKRATLLFRDLERLKRLLQNQWRPVQLVFAGKAHPADEPGRYFIHEVLSFCHDHKLG
                     GHVAFVEDYDMHMAKFLVQGVDVWLNTPRYPMEASGTSGQKAALNGVLHFSVLDGWWD
                     EGYNGANGWAIEPLTGNHDVQAQDSHDAEQLYHLLEQETVPLYYQRDLDGIPRGWIQL
                     IKESLRTVAPRFCTKRMIKEYVNTLYAPAMARTPSAW"
     gene            4273..5487
                     /locus_tag="VI359_09855"
     CDS             4273..5487
                     /locus_tag="VI359_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY6974596.1"
                     /translation="MKLWRSVPASSQPSKPFLRLVLQSFLKLGHAEDALALYQRLVPT
                     DQSDDPVLLRAVALSMLTSHVRDPKEHVRIAAFTTLADLGLKETRPVLEDGLLDTSVL
                     VRARAVEAIGRAGLAAASGPVRRALQDEMPAVRIAAMNVLGDAQATDIIPRLLEVARN
                     EDSPEGIFACAALYHLGKRDMLTDITSAATLPDPEIRMAAFGVLGRLRRPSSAAVLAH
                     GVYDPEPSVRAFAAGALGEYGKVDGVAPLTHALGDEVARVRGVAAVSLGRLGVRDTRP
                     LLQALLRDPSMHVRASAIEGLLRLGDTSVFLIVTDLARHPDPSVRSATAQALGATSEK
                     QGLAILQTLLYDQQPQPRLAAAKALGKVSGAVVPWLKRGLQDSDEAVRLTVAGSLIQQ
                     LSHSPKPAQRRS"
     gene            5499..>5779
                     /locus_tag="VI359_09860"
     CDS             5499..>5779
                     /locus_tag="VI359_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974597.1"
                     /translation="MLKAFAIIALAIASFGIGFYFGQRPVGTLQNTISDLQQSIKDLS
                     RNVFDTTMGIERDLRRRQGLLDTKSHIVQAKADLIDKNFGEAAKELADAA"
BASE COUNT         1285 a         1667 c         1601 g         1226 t
ORIGIN      
        1 cgaatcatcg ccgaccacca tcacgttctg gtgcgtggcc gcgagtagac gaatcagatc
       61 tccctgcaaa cgattcgtgt cttgatattc atcgacgaga atatagcggt acaacgaagc
      121 gatggactgc ctagccgtat catcctgcat cagcagccgc cgcaacagca caagcaagtc
      181 atcataatcc accagttgct tctgacgctt ggccgtctga tatcccttct gcagtcgttc
      241 cagagcatcg agatgatcag cgaaatggct aaactcctcc agcaggattt cgccgatcgg
      301 gcgcagcgta ttttcagcct tactgaagat ctcggcgatg gtgcccttcc gtgggaaccg
      361 cttgtctttt tcgttcaaac cgagctggga ccgaaccaag gctacgaggt cttcggcatc
      421 acttcggtcg agaatggtaa agcccggctc caggccgatc tggcgcccga atcgcctgag
      481 cagcatattg gcgaccgagt gaaacgtgcc tccacacaca cgctcgctgc gccctccgat
      541 cagcgccccc gcgcgctcta gcatctcctg cgcggatttc ctggtaaagg tcagtaacaa
      601 gatactggca ggatccaccc ctgaatcgat caaataggcg acgcggtaca ctagcgttcg
      661 cgtcttaccg ctgccggccc cagcgatcac gagcgccggc ccgttgccgg ccgtcaccgc
      721 cgcatgttgc tgggggttca attccgccgc atagttgatg gagagccgtc gcgggatcgc
      781 ctcatccgac gatcgcttca acacataggg tgtgatgtct cggttcatgg tagtgatcag
      841 aaagtggtcg cgctgtataa cataccaccc aggacctttc aacgaacagt cgatggagaa
      901 ggagtagaaa gtgcaactgc ggggcggctg ctggcacaac aatcgttcat cgaggtgccc
      961 attggagact caagcagaac ttgtatataa tggtgatctt ttttacgagg tggtaatggc
     1021 agcgacaaaa agtccctaca gcccgcaact ccgcacgatt gccgaattaa tgcttgccgt
     1081 agcaggggaa tccgtgacca tgatgaaacg gaatgcgccc caacaggcat tgaagctcaa
     1141 gcgtcaaacc gaatggaaaa tctatttgga attcctcaag gtcatgttca atctaaccga
     1201 tcgcttgtcg gttctgcatc ttcctcttaa agaccagccg gaattcatga acgacctgga
     1261 agatgccgtc acattgcagt taaaaaccgt ccttgagccc gcatttgcgt ctggcggaga
     1321 ccagacagaa attatgctga ccatcagtgg agcggtggcg gagagccgac agatttatga
     1381 gaaatatcga tttctgatta ccgaggacag ccccgccaaa aacgatctct tgaaagattt
     1441 tggcgatcgt gtcgctgatg tcatgggcgt tgcgggtgat gggcaggtct cttctgccgc
     1501 gaccctctgt gtcagcgcgg tcattccggc tctcacgtca atcttggctg gagacgctcc
     1561 accgacacaa gacacactcc aagcgcttca aaatcagtca tcgagctcta ccgagaagcc
     1621 ggcttcatcg actcccaaag gtcccgtcgg cagcgaaatc aagcttgtca gcgtgatgtc
     1681 cagcgttgtc ggagaagaag tggaaacccg atggggcctc catccgcgat tccgccagga
     1741 tcttaaaccg gacgaagtcc agcagttgac taaactgatg aaccgagttt cgaaaattct
     1801 tggcgaacgc tatgccaccg tggcattctc cgatgaatgg gcgtcatggc ataaggccgg
     1861 tcacgcctaa tcgcactcac ctttgaacgg ggcgcatttc ttatctcact gacacgcgca
     1921 gggaggtttc gtggacaatc caaagcagac agaatctctt ccgaacggca ttccgtccgg
     1981 attgacgcgt ctgcccgaac tcgcacagaa tttatggtgg agttggaccc ctgaggcgcg
     2041 gcggctcttt gaagtgattg atccaaccgt gtgggtgctc tgtcaccata atcccgttaa
     2101 actcttgacc gaggtcaaac cggagcgact cacacagttg gctgaagacc cgagttatgt
     2161 tcgccagtat tcggccgtgt tgaaactctt cgaccagtac caccagaacg gacgaagctg
     2221 gcttaagtcg catcatgccg agttagccgg caccaccatc gcatatttct ccgcggaatt
     2281 cgggctgcac agttccattc cgatctatag cggcgggctg ggaatcttgg ccggtgacca
     2341 ctgtaaggag gccagcgatc tcggtgtccc gctggtcgga atcggattta tgtacccgca
     2401 aggttatttc cgacagcgaa ttaccgcgga agggtggcag gaagccgcct atgccccatt
     2461 caatcgagat gactcgccta tccgcccagc cctcacacca tcaggtactc cctgcagaat
     2521 caccgtcgaa atgggagatc gcattgtctc ggccattgtc tggaaagtgc tcgtcggtcg
     2581 agtcacactc tatttgatcg acacggacgt accggagaac agcccggaga atcgtgctct
     2641 ctcggctcgc ctctacggag gcgatcaaga aatacggctt tgccaggaaa ttctcctggg
     2701 catcggaggc gtccgtatgc tccgcacatt ggggatcaac ccgacggtct ggcatgccaa
     2761 tgaaggtcat tcggcatttt tgacgctcga gcgagtgcgc gagctcgtcc agaagggtcg
     2821 gtcgcacgcc gaagcgagcg aaacggttcg ccagagtacc gtgtttacga cccacacgcc
     2881 agtccctgct ggtcacgacg tattccccca tcacctaatg gatcggtact tcaatggcta
     2941 ttgggaacag atcggcttgt cccgggacga atttctcggc cttggggata gccccagttc
     3001 gcaccaacag ggattcaaca tgaccgcgct ggcgatccga ctttcggcca acgtgaatgg
     3061 agtgagtcgt gaacatggcc gtgtcacccg tgagatgtgg caacattttt ggcctgggtt
     3121 ggcaatcgat ctggtaccga ttcggagtgt cacgaacggt gttcacgccc ccacatggat
     3181 tgcggcagaa gtcagtcagc tctatggcaa atacctcgga gcggattggg tcgacaaatg
     3241 cgacgactcg gccatatggc agcgtgtgtt ggatattccc gatcacgaac tctgggctgt
     3301 tcgccaaacg atgaagcgca aattgatgag cttcatccgg gaacgagctc gaagcggatg
     3361 gatgcagggg cacctgcaag caacgcaggt gctaagccgc ggcacactgc tcgaccctga
     3421 agccctcacg atcggctttg ccaggcggtt tgccacatac aaacgcgcca ccttactctt
     3481 ccgcgacctc gaacgactca aacgtctgct gcagaatcag tggcgacccg ttcagcttgt
     3541 cttcgccggc aaagcccacc ccgctgatga gccggggcgc tatttcattc atgaggtgct
     3601 gagcttctgc catgaccaca agttaggagg ccatgtcgca tttgtggaag actacgatat
     3661 gcacatggcc aagttcctgg tccaaggtgt ggacgtttgg ctgaataccc cgcgatatcc
     3721 gatggaagcc agtgggacga gcgggcaaaa ggcggctctc aacggtgtct tgcatttcag
     3781 cgtcttggac ggctggtggg acgaagggta caacggcgcg aacggatggg caatcgagcc
     3841 ccttacggga aatcatgacg tccaggctca agattctcac gatgcggaac aactatacca
     3901 tcttttagag caggaaacgg tgccactgta ttaccagcgg gatttggacg gaatccctcg
     3961 cggatggatt caactcatca aagagagcct ccgcactgtc gctcctcgct tctgcacgaa
     4021 gcggatgatt aaggaatacg tgaatacgct atacgcgccc gccatggcac gcaccccgtc
     4081 tgcatggtga ccgaacgcct gccaacacct ctctcatcga tgcgttttcg agtgatcgta
     4141 tacagcctac tgtgtgttga gctcttcgcg gccatggttt cagcccctgt tttgtgcgct
     4201 gcagactcag ccgtcgcggc aattgaacgt gacgcgacga gggccttcaa caattcagac
     4261 tacgacttgg tcttgaaact gtggcgctcg gttcccgctt cgtcacaacc ttcaaaacca
     4321 tttctccggc tcgtgctcca gagctttctg aagctgggac atgcggaaga cgccctcgcg
     4381 ctgtaccaac gactagttcc gacagatcaa tcagatgatc ccgttctctt gagggccgta
     4441 gcactgagca tgctcacgag tcatgtgcgc gatcccaaag aacatgtgcg aatcgccgcc
     4501 tttacaaccc ttgcagacct gggcctcaaa gaaacgcggc ccgtgctgga ggatggtctc
     4561 ctcgatactt cagtcttggt gcgcgcgcgt gcggtggaag ccattggcag agccggcctg
     4621 gccgctgcgt ccggtcctgt gcgcagagcg cttcaggatg agatgccggc cgttcgtatt
     4681 gcagccatga acgtgttggg agacgcacag gccacggaca tcattccccg attgctggaa
     4741 gtcgcaagaa acgaggacag tcccgagggc atttttgcat gcgcagcact ctaccatctc
     4801 ggcaaacgcg atatgctcac ggacatcacc tccgctgcaa cgttgccgga tccggaaatc
     4861 cgcatggcgg cattcggagt cctgggccgg ctgaggcgcc cctcaagtgc ggcagttctg
     4921 gctcatggcg tctatgaccc ggagccgtct gtgcgcgcct tcgccgcggg cgcgttaggc
     4981 gaatatggaa aagtggatgg cgtggctcct ctgacccatg cgttgggcga cgaagtcgct
     5041 cgggtacgag gagtcgccgc ggtcagcctc gggcgactgg gtgtgcgtga cacgcgtcct
     5101 ttgctccagg cactgcttcg agatccgagt atgcacgtac gcgcaagcgc gatcgaaggg
     5161 ctcctgcgcc tcggggatac ctccgttttc ttgatcgtca ccgatcttgc tcgtcacccc
     5221 gatccatcag tccgctcggc gaccgcccag gccttgggag caacctccga gaaacagggc
     5281 ctggcgattc tccaaactct cctatatgac cagcaaccgc aaccacgact ggccgcggcg
     5341 aaagcgcttg ggaaagtatc cggtgctgtg gtaccgtggc tcaagagagg ccttcaagat
     5401 tcggacgaag cggtccgcct gacagtggct ggcagtctga tccaacagct gtcgcattct
     5461 ccaaagccgg cgcaacgtcg gtcgtagaaa ggagccctat gctcaaggct ttcgccatca
     5521 tcgccctggc tatcgcctcg ttcggaatag gcttttactt cggacagcga ccggtgggta
     5581 ccctacaaaa cacgatctct gacttgcagc agtcgatcaa agacctgtcg cggaacgtct
     5641 tcgataccac aatgggaatc gagcgcgatc ttcgacgacg ccaaggtttg ctcgacacga
     5701 aatcgcacat cgtccaggca aaagccgatc tcattgataa gaattttgga gaagccgcaa
     5761 aggaactggc ggatgcggc
//
LOCUS       DATDNW010000412         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_543913, whole genome shotgun sequence.
ACCESSION   DATDNW010000412 DATDNW010000000
VERSION     DATDNW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543913"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VI359_09865"
     CDS             complement(<1..705)
                     /locus_tag="VI359_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY6974598.1"
                     /translation="MYEGSRGGTALEDVPTAVAVKKRRSSPQLVWLIPIVAALIGGWL
                     AVKTILEKGPTVTITFKTAEGIEAGKTKAKYKNIDIGEVKSVAFTKDLERVVVTVEFH
                     KGVEPHLVEDVRFWIAKPRIAGGQVSGIGTLFSGSYIEMDRGKSSVPRREFEGLEVAP
                     FVTGDVPGRQFILQGKDLGSHEPGTPVLFRRIAVGKVAASELKQDGSGVTLKIFVESP
                     YDKFVTANTRFWDVSGI"
     gene            complement(698..1330)
                     /locus_tag="VI359_09870"
     CDS             complement(698..1330)
                     /locus_tag="VI359_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY6974599.1"
                     /translation="MSESAVTASQMGLCACHGCGGLFQLIPGVHEMRCRGCGASLHLR
                     KPNSISRTWAYLIAASIMYVPANVLPIMKTGSLFGSQSDTIMSGVVYLWHSGSWHLAL
                     VVFIASVLVPLAKIISLTYLVITVQLGWTERALERTKLYRVLEFVGPWSMLDVYVVTI
                     LVALVQLKSLATIQPGPAAAAFGAVVVLTMFAAISFDPRLIWEPEEKHHV"
     gene            complement(1327..1935)
                     /locus_tag="VI359_09875"
     CDS             complement(1327..1935)
                     /locus_tag="VI359_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY6974600.1"
                     /translation="MAELALTACHECDLLQREPILPPGGIACCERCNAVLFRHTPDLV
                     DRGLAWTLAAAILFVVANFFPIVGLEVQGTLNATSLYGAVERIWDNDMQGVAALVFIT
                     TILIPAMEISLMLYLLFPLKFGWLPGETKLILRVLQSVRPWSMTQVFILGVLVALVKL
                     AHLAHVVPGVALWAFGGLILSLTAAIANFNAHEVWKLLKESE"
     gene            complement(1987..>2604)
                     /locus_tag="VI359_09880"
     CDS             complement(1987..>2604)
                     /locus_tag="VI359_09880"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEY6974601.1"
                     /translation="KQSGFLGDYSLLKKGGEGEAALVYRKNDNDRPKWRSYSQILLEP
                     VQIWKGEKTELNPEDAEYLAKIVWSRLDEELRKDYKMTSSPGVGVLRIQTAVTEAGTN
                     MPVLDLVTTLYPGTRLISEGKRWASGTESFVGAASVEVKVTDSQSGELLGAAVDRRGG
                     GKYVTKGFQKWTDVEEAFTYWAKQLRWRLCLERGVPGCDLMKPEA"
BASE COUNT          604 a          807 c          655 g          538 t
ORIGIN      
        1 aattccgctc acgtcccaga agcgcgtgtt ggcggtcacg aatttgtcat acggcgactc
       61 gacgaagatt ttcaatgtga cgcccgagcc gtcctgcttc agttcactcg ctgccacctt
      121 tcccaccgca atccgccgaa acaagaccgg tgttccgggc tcgtgcgacc cgaggtcctt
      181 gccttggagg atgaactggc ggcccggcac gtcgcccgtt acgaatggcg ccacttcaag
      241 gccttcaaac tcccttcgag ggactgagga cttgccgcga tccatctcaa tgtacgatcc
      301 cgaaaacagg gtcccgatcc ccgatacctg cccccccgct atcctcggtt tagcgatcca
      361 gaacctgaca tcttccacga gatggggctc gaccccctta tgaaactcca ccgtcacgac
      421 aacgcgctca agatccttgg tgaaggccac ggatttcact tcgccgatgt caatattttt
      481 gtacttggcc tttgtcttgc ctgcctcgat cccctctgcc gtcttaaacg tgatggtcac
      541 cgtggggcct ttctcgagaa tcgttttgac ggccaaccac ccaccgatca aggcagcaac
      601 tatgggaatg agccatacga gctgcggcga cgaccgtcgc tttttaacag ccaccgccgt
      661 tggaacgtcc tctaacgctg tgccaccacg agatccttca tacatgatgt ttctcctcag
      721 gctcccagat gagcctcgga tcaaacgaga ttgcagcaaa cattgtcaag accaccacag
      781 cgccgaacgc agccgccgcc ggacctggtt gaatcgtcgc aagcgatttg agttgcacaa
      841 gcgccacgag aatcgtcacc acgtatacgt ccagcatcga ccagggaccg acgaactcaa
      901 gcacccggta aagcttcgtg cgttcaagcg ctcgttcagt ccaccccaac tgtacggtaa
      961 tgacgagata cgtgagggag atgatttttg cgaggggcac aaggacgctg gcgatgaaga
     1021 ccaccaatgc aagatgccac gagcccgagt gccagaggta caccacaccg ctcatgatcg
     1081 tgtccgattg agacccgaac aacgagccag ttttcatgat cggtaggacg ttggccggaa
     1141 catacatgat agaggcggcg ataaggtacg cccacgttcg cgagatactg ttgggtttgc
     1201 gaagatgaag cgatgcgcca cagccgcgac aacgcatttc gtgaactccg ggaatcagtt
     1261 ggaacaagcc gccacatccg tggcaggcgc acagtcccat ttgactcgca gtcaccgcag
     1321 actcactcat tcgctctcct tcagcaattt ccacacctca tgcgcgttga aattagctat
     1381 cgccgcagtc agtgatagaa tcagcccgcc aaaggcccag agggcgacac caggcacgac
     1441 atgcgcgagg tgtgcaagct taaccaacgc caccagcaca ccgaggatga acacctgcgt
     1501 catgctccac ggtctcacag attgcaggac gcggaggatc aacttcgtct ccccaggcaa
     1561 ccatccaaac ttcaatggaa aaagcagata cagcatcaac gaaatctcca tagcagggat
     1621 caggattgtt gtgataaaga cgagcgctgc cacaccttgc atgtcattgt cccagatccg
     1681 ttccaccgcc ccgtagaggc tggtcgcgtt cagggtgcct tgcacctcca aacccacaat
     1741 cggaaagaaa ttcgcgacga caaagaggat ggctgctgcg agcgtccagg cgagtccgcg
     1801 atcgaccagg tctggtgtgt ggcgaaacag caccgcattg catcgctcgc aacaggcgat
     1861 gccacctggc gggaggatcg gctcccgttg caagagatcg cattcgtgac aggcggtcaa
     1921 ggcaagctca gccatgccgt ataaccatac cgacgcatgc cggttccttc atataacccc
     1981 aaagctctat gcttccggct tcatcaggtc gcagccaggc acccctcgct caaggcacaa
     2041 tcgccagcga agctgtttgg cccaatacgt aaatgcctct tctacgtcag tccacttctg
     2101 aaaccccttg gtgacatatt tccctccgcc gcgacgatcg acggcagcgc ccaacagctc
     2161 gccggactga gagtcagtga ctttgacttc gacagatgcg gcgcccacga acgattcggt
     2221 accgctcgcc catcgttttc cttccgaaat gagacgtgtt cctggataga gagtggtgac
     2281 gagatcgagg acgggcatgt tggttcccgc ttccgtgacc gcagtctgga tacggaggac
     2341 cccgacacca gggctactcg tcattttgta atctttgcga agctcttcat cgagtcgtga
     2401 ccagacaatt ttcgccagat actctgcgtc ttctggattg agctctgtct tctcaccctt
     2461 ccaaatttgc accggttcca ggagaatctg agaataggac cgccacttgg gacggtcatt
     2521 gtcgttctta cgatacacca gggccgcttc cccctctcca cctttcttga gcagcgaata
     2581 gtcaccgaga aatccagatt gttt
//
LOCUS       DATDNW010000413         3982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_952794, whole genome shotgun sequence.
ACCESSION   DATDNW010000413 DATDNW010000000
VERSION     DATDNW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952794"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..285
                     /locus_tag="VI359_09885"
     CDS             112..285
                     /locus_tag="VI359_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974602.1"
                     /translation="MECPKCKGLMMLERFSDFFLIFYAWKCINCGAIIDRTISNNRRK
                     SLAVRDSQPVATR"
     gene            578..1138
                     /gene="rsmD"
                     /locus_tag="VI359_09890"
     CDS             578..1138
                     /gene="rsmD"
                     /locus_tag="VI359_09890"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY6974603.1"
                     /translation="MRVIAGSHRGRRLSGPRGATLRPTSDKVREALFSILGSRVAGGR
                     FLDLYAGTGAVGIEALSRGVERATFVETNPSIVSLLRKNLDACDLAERSQVWVGRTAA
                     FLTRHEVWDGPYSVVFADPPYAARSEIDVLAGLGQAGLLSHDAVIVIEQASLDESPER
                     IGPAMLTRRYEYGDTALSLYHKVTQQ"
     gene            1146..1634
                     /gene="coaD"
                     /locus_tag="VI359_09895"
     CDS             1146..1634
                     /gene="coaD"
                     /locus_tag="VI359_09895"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY6974604.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVVVAVAPNPSKHPL
                     FNLDERLEMVKLVMKDLGQVEVTAFEGLLVDYVEQSGAHAIIRGLRAISDFEHEFQMA
                     LINRKLAKRVETVFLMPSEEYSYLSSTIIKDVANHGGSLTEFLHPEVARRLQKRIRSL
                     KP"
     gene            1631..2824
                     /locus_tag="VI359_09900"
     CDS             1631..2824
                     /locus_tag="VI359_09900"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY6974605.1"
                     /translation="MKLAARAGRITPSPTLAMAATAKAMAAQGIDVIDFSSGEPDFDT
                     PEPVKAAAEAAIRSGFTKYTPSSGTDELRAAIAEKLLVEQGLRYEKAQILVSCGAKHS
                     LYNLAEALLEAGDEVIIPVPYWVSYSDQVLLNDATPVLLPTREEEGYTIHADQLERCI
                     TPRTKAIIVNSPCNPTGATYDRATLERIASIAVRRDILIISDEIYEKVVYEGTHHVSI
                     ASLGPEVAARTVVINGVSKAYAMTGWRIGYAAGPKQLLTAMANIQSQSTSNPCSISQK
                     AAVAALRLGGSFTEKMVAEFDQRRRVMVERLNAMPGVRCSMPTGAFYAFPNVQGLLGR
                     QGPGGAIKTPTELANYLLHDAKVAVVPGEPFGSPHHLRMSYATGMNAIRQGMDRLESA
                     FAKLT"
     gene            2906..3277
                     /locus_tag="VI359_09905"
     CDS             2906..3277
                     /locus_tag="VI359_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY6974606.1"
                     /translation="MPIYEYVCDGCAYRFERKQSIKDDPVTTCERCGKSVTRLISSPA
                     IMFKGSGWYVTDYSDKLKPPAGSEPAAPGVAEKGAADKKDSSPSSSSSQAPSPSSSSD
                     SPSSSPSTSSGTSTPTSTTTK"
     gene            complement(3299..3712)
                     /locus_tag="VI359_09910"
     CDS             complement(3299..3712)
                     /locus_tag="VI359_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974607.1"
                     /translation="MGDGRERGGWIGFCVVAGIGLMLAGCDYWPPALQTQIEQLRSEV
                     QAATAERAQLQNQLTALTKVKDDLQAQVDDLSRANREKTSMINSLQSSLAAAQERLAK
                     QAKPAPAKASTAKAPTKSSSKVAAKAPAKKKLTTH"
     gene            complement(3765..>3982)
                     /locus_tag="VI359_09915"
     CDS             complement(3765..>3982)
                     /locus_tag="VI359_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEY6974608.1"
                     /translation="ESAARTAQEVEQALGDEIDLIIDAGAMPGGLPSTVIKVQGQTQI
                     VREGTIPRACIRETLRARGFFLNEGQM"
BASE COUNT          835 a         1166 c         1096 g          885 t
ORIGIN      
        1 tctgacaaat acgtagcccc taacatgttg aaattacgta ttgcgacaat ttggtgtagg
       61 tcttgcttgg tttcgccgac agctgtgcac gtaaccacca gggaggattc gatggaatgc
      121 ccgaagtgca aaggcctcat gatgctagaa cggttctccg atttcttcct catcttctac
      181 gcctggaaat gcattaactg cggcgccatc atcgaccgca caatttccaa caatcgacgg
      241 aagagtctcg ccgtgcgaga ctctcaacca gtcgccacgc ggtaacacgc aacgcgacga
      301 tcccgcagga tagtctgagg ttcttaccgg atcgtcgctg gtctccccac gatcgcagcc
      361 ggcagcagag ggcacagcca gccggcgcgg cattatagcc atccttcttc gatatcgtca
      421 aagtaccgac gtcacattcc tggtattctc ctctacccat gcgcacagaa cagagggtcg
      481 caggcttgct ccgttggcag cggggcagcc tcgcttgacc ccttcaaaaa cgctgtgtta
      541 gagtcaaccg ctctggagtt atacctcgtc cgaaacgatg cgagtcattg ctgggtccca
      601 tcgaggtcgg cgcttgagtg gaccgcgagg ggctactctt cgtcccacat cagataaggt
      661 ccgagaggcg cttttttcaa ttctcggttc acgtgttgcg gggggacggt tcctagacct
      721 ctacgctggt accggagcgg tcgggatcga agccctaagc cgaggtgtcg agagggccac
      781 gtttgttgag acaaacccca gcatcgtctc gctgcttcgc aaaaatcttg atgcatgtga
      841 tctcgctgaa cggtcgcagg tatgggttgg acggacggcc gcctttctca cgcgccacga
      901 ggtgtgggac ggtccctata gtgtggtgtt tgcggatcct ccttatgccg ctcgatcgga
      961 aatcgatgtg cttgctggcc tcggacaggc agggctgtta agccatgatg cagtaatcgt
     1021 catcgagcag gcctctctag acgaatctcc tgagagaata gggccagcga tgctgacacg
     1081 ccgctacgag tatggcgata ccgcgttatc tctctatcac aaggtgaccc agcagtgagc
     1141 cccatatgaa gatcggcatc tatcctggca cctttgaccc cattactcac ggccataccg
     1201 atatcattac gcgcagtctg cgtgtgtttg acaaagtggt ggtcgcagtg gcgccgaatc
     1261 cgtccaagca tcctttattc aacctggatg aacgactgga gatggtgaag ctggtgatga
     1321 aagacctggg ccaagttgag gtgacggcgt tcgaaggact cctggtcgat tacgttgaac
     1381 agtcaggcgc ccatgccatc attcgcggtc tacgtgcaat ctccgatttc gaacacgagt
     1441 ttcaaatggc gttaattaac cgcaagctgg ccaaacgggt cgaaacagtt ttcttgatgc
     1501 cgagcgagga atattcgtac ctctcgtcga ccatcattaa agacgtcgcg aaccacggcg
     1561 gttctctgac cgaatttctt caccccgaag tcgcacggcg gctccaaaaa cggatcagga
     1621 gtctcaaacc atgaagctgg cagcacgggc tggacgcatc actccctccc ccacccttgc
     1681 gatggcggcg acagccaaag ccatggcagc gcaaggcatc gacgttatcg atttttcttc
     1741 tggcgagccg gatttcgaca caccggaacc ggtcaaagcg gctgcggagg cagccattcg
     1801 ctccggcttc acgaaataca ctccgtcgtc cgggaccgac gaactgcgtg cggcgattgc
     1861 cgaaaaactg ctagtcgaac agggtctgcg ctatgaaaaa gcacagattc tcgtgtcgtg
     1921 cggggcgaaa cactcgctct acaatctcgc tgaggcgttg cttgaggctg gcgacgaggt
     1981 gattatccct gttccgtact gggtatccta ttccgaccag gtgctcctga acgacgcaac
     2041 tccggtcctg ctgccgacgc gggaggagga aggctacacg atccatgccg atcagctgga
     2101 gcgctgcatc acgccgcgaa ccaaagcgat catcgtcaat agtccgtgca accccacagg
     2161 agcgacctac gaccgggcga cattggagcg gattgcgtcg attgccgtcc gacgagatat
     2221 tctcattatc tctgatgaga tctacgagaa ggtcgtgtac gaggggacgc accacgtcag
     2281 tattgcctcg ctgggtccgg aggttgccgc gcgtaccgtg gtcatcaacg gtgtgtcgaa
     2341 agcctatgcg atgactggtt ggcgcatcgg ttacgctgct ggtccaaaac aactgctcac
     2401 cgccatggcc aacattcaga gccagagcac atccaatccg tgctcgattt cacagaaggc
     2461 tgcggtggct gcacttcggc tcggcggatc gttcacggag aagatggtgg cggaattcga
     2521 tcagcgtcga cgtgttatgg tcgaacgact caatgcgatg cccggggtgc gatgttcgat
     2581 gccgaccggc gccttctatg cgtttcccaa tgtgcaggga ttactcggtc gacagggacc
     2641 cggcggcgcc atcaagaccc cgactgagct ggctaactac ctgcttcacg acgccaaggt
     2701 cgcagtggtg ccaggggagc ccttcgggag tcctcatcat cttcggatgt cgtatgcgac
     2761 ggggatgaat gctattcgcc aagggatgga tcgtttggaa tcggcatttg cgaaactgac
     2821 gtgagatcat tcgatactgg tgtgcgcgac agtcgtcgcg tcagcatgag agcaacaaga
     2881 ctatactcgc aaggaggatc gttacgtgcc gatctatgaa tatgtgtgcg acggctgtgc
     2941 gtatcggttc gaacggaaac aaagcatcaa agatgacccg gtgacgacct gcgaacggtg
     3001 cgggaaatcc gttacgcgat tgatttcctc ccccgctatc atgttcaaag gcagcggctg
     3061 gtacgtgact gactactcgg ataagctcaa gccgcccgcc ggcagtgagc ctgcggcccc
     3121 gggagtagcc gaaaagggag ccgctgacaa gaaagacagt tcaccaagtt cctcgtcgtc
     3181 gcaggccccc tcaccttcct catcttcgga cagtccttcg agttccccct cgacgtcctc
     3241 aggaacttcg acaccgacct caaccaccac gaaataggag ctgaccggct acgtcacgct
     3301 agtgtgtcgt cagtttcttc ttcgccggag ccttggcggc aacctttgac gatgatttgg
     3361 tcggcgcttt ggcggtcgat gctttcgccg gtgccggctt ggcctgcttg gccaagcgct
     3421 cctgcgccgc cgcgaggctg ctttgcaggc tattgatcat ggaagttttc tcgcgatttg
     3481 cccgactcaa gtcatcgacc tgtgcctgta gatcgtcctt gaccttcgtg agcgctgtca
     3541 gttgattttg caactgtgcc cgttcggctg tcgccgcttg cacctcggac cgtagttgtt
     3601 cgatctgagt ttgcaacgcc gggggccagt aatcacaccc cgccagcatc aaccctattc
     3661 ctgccacaac acagaatcct atccatccgc cgcgctcccg accatctccc atagttctcc
     3721 tcctttctgc cctgtgcgta aatgcaagct gccaatgttg tatatcacat ctgcccctcg
     3781 ttgaggaaaa atcctcgcgc gcgcaacgtc tctcggatgc aggctcgcgg gatcgttcct
     3841 tcacgcacaa tctgtgtctg tccctggacc ttaattacgg ttgatggcaa cccgccgggc
     3901 attgcgccag catcgatgat caaatcgatc tcgtcaccca acgcctgctc tacctcttga
     3961 gccgttcgag ccgcgctctc cc
//
LOCUS       DATDNW010000414        16844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_955205, whole genome shotgun sequence.
ACCESSION   DATDNW010000414 DATDNW010000000
VERSION     DATDNW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955205"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VI359_09920"
     CDS             complement(<1..1285)
                     /locus_tag="VI359_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974609.1"
                     /translation="MKRSDWAIRATGLSKKFSMDLRQSMLYGLKDSLQRLTTRQTSTG
                     ALRPGEFWALKEVSFELARGESLGIMGVNGSGKTTLLRILTGVFPPDAGEVSLQGRVG
                     SLIAAGAGFAPMLTGRENIYVNGSLMGMTKEEIDDRLEAILEFSGVGAFVDSPAKHYS
                     SGMLVRLGFAVAAMSEPDILLVDEVLAVGDLNFQKKCYDYLHRLKAKGTSIILVSHSV
                     GAVWAICDQGLFLHQGEVKRSGSVEDVIRAYDDQNAALLQPASLSHDGRTGRVDQLSR
                     QNSAGPTGGTGDAYIKNVRVCSLAGDDTARDALEFHESFALEADLEVLTKLDEPIFRF
                     TIDAAHYRFIVSVDSYEQNFRLDELKPGRYCLRVEVPDQNLMPGAYTVNVNVCRKGFG
                     NHLFMWNGAARFQVKTPQHRLLYSEPNAVMFLEAKF"
     gene            complement(1282..2166)
                     /locus_tag="VI359_09925"
     CDS             complement(1282..2166)
                     /locus_tag="VI359_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY6974610.1"
                     /translation="MMSASFNKAAGQPGDLPIVTYSAVNMRVTIGSSLAQAVTEIWAT
                     REVIWRLFVRDFTQQFRQKLLGYLWMVIGPMVNAAGFVFLYVAGILKPGDMVIPYPVF
                     VLFGTYLWGMMTGVLTVVSGSLHAQGELITRTNIPKIALALTGMGNFAFALVTNLVVL
                     LGIMIYIGFPPSWLILLFPLLMVPLMALGIGLGLIFAVIGVMARDMTTLATAAFNLLM
                     LVTPVVYVGKVDSPALQRMMEYNPLTYLIDVPRTLVMTGVLPHPLAYAAASALAIVML
                     VLGIYAFYLIQDKVAERL"
     gene            complement(2163..2942)
                     /locus_tag="VI359_09930"
     CDS             complement(2163..2942)
                     /locus_tag="VI359_09930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY6974611.1"
                     /translation="MNRTFQSGLLSLYRLSHATGVLSTSWGRSLFDRVYTGYKALLEA
                     PHINLLATMVVQGTHIIDVGAFHGFFTVKFGRWVSQTGKVIAIEPEPENYARLRRIVA
                     TAGLNDRVETVQAAVAERSGEVRLELNPLSPVDHKLGEEGIRVKALTIDDLLEARGWP
                     PVSLMKIDVQGAEEKVLAGAVRTLSTFQPSLFIEVSDETLARYGSSAERLLRTLIEKG
                     YTIHRLSRTSISTALTIVEALSIQQSRGYADFLFLHSRRGS"
     gene            complement(3018..3986)
                     /locus_tag="VI359_09935"
     CDS             complement(3018..3986)
                     /locus_tag="VI359_09935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974612.1"
                     /translation="MNGSAAPITVEEVLCPTCAGNEYDVAAEGPDYDNHCCGDQQFTL
                     VQCHHCRTFYLNPRPTVDMLNVIYAPDKYYSYSFAEQGNSWVQQARQGRDRQKIQDVL
                     RLLDKRSHEITVLDIGAGDGTLLQAFRSSGVHPENLYGLDIEPQAVERLRSRGFQGVT
                     GRVEEADLLPSHFDVVTMIQVIEHVANPRNVVRSVYRILRPGGIFLIETPNAASWDRP
                     FFARKTWGGYHFPRHWTLWTPVTLSAMLSKEGFHVVGSETPVAAALWTWSVNHVLQDR
                     GWPRTLTECFSLNNPIALAIFWLVDLIPSLMGRSANMRVLARKPLS"
     gene            complement(4014..5075)
                     /locus_tag="VI359_09940"
     CDS             complement(4014..5075)
                     /locus_tag="VI359_09940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEY6974613.1"
                     /translation="MPANEPRAGRLAGISELFEEWPSDQPNEWLSRDEIASLHTIRYG
                     RVVADISLSWALVLLALHAVIWTSSFTVGATAFILIGCLQNAFILWTHEASHYNLTRN
                     KRVNDWLSDMFISGPTGVTVGQYRWQHVRHHRYLGDPRKEIEPLAWICIRSNNLYLEI
                     LRHLAGIYGLHVIQRYQKQEHDLRYSNLPKRSVASLVGFVLGNGILFGLCAIHGKWYL
                     YFLLWVLPLFTVALLIGNFRTIVEHQPSSDVCDVGLVRIPAVTRVVRSGWLERLLIAP
                     VGFYYHFEHHLFPGVPYHRLKEVRKLLVQRGCFEREHIVWANGYLRTIWNLAMKPGYG
                     LRIERAPESRPLPKASIGR"
     gene            complement(5163..6008)
                     /locus_tag="VI359_09945"
     CDS             complement(5163..6008)
                     /locus_tag="VI359_09945"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY6974614.1"
                     /translation="MIRSRTKIALARSAYWAIMLARRTVGLGSRVSTIRDGLRWELDL
                     QEGIDLAIYLFGMFERETVMACRRWIKPGDVVFDIGANVGAHTLQCARSVGATGKVVG
                     FEPTRYAFTKLVRNVACNPELVERIITEQIMFGEKPGERIPSLLYSSWPLMGHTDVHD
                     KHCGRLMDTEGAQATSLDSYVERTGIKRVGFIKLDVDGHECAVLRGGSTTLKRDRPVM
                     VMELAPYVLDETGTSLEDLCTILSCQEYRLADMTTGHPLSLDPRELKQMIPDGCGVNV
                     LALPN"
     gene            complement(6034..6765)
                     /locus_tag="VI359_09950"
     CDS             complement(6034..6765)
                     /locus_tag="VI359_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974615.1"
                     /translation="MGTDSRLRHYRTVWESKPVLRALYQDYYRQIIAACRPGLTLEIG
                     GGSGNLKAFASHVISTDILPATWLDAVADAQALPFRDRSFDNIVMFDVLHHLPCPRLF
                     LAEAERLLRPGGFLIALEPAITPLSWLFYRCFHPEPVQLADDPLARSVLRANQNPYDG
                     NQAIPTLLFLRNRQRLEHEFRSLRVRTIKWLSLFVYPLSGGFRSWSLLPSWLVEPLLH
                     MEEVLLPLLGSVMAFRALVIVQRAE"
     gene            complement(6766..8217)
                     /locus_tag="VI359_09955"
     CDS             complement(6766..8217)
                     /locus_tag="VI359_09955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6974616.1"
                     /translation="MLAVCDQLLVSSRKTAVRQLSDELASARDRWIEKNAFYYEDDRR
                     YMQFLIPPGQRVLDLGCGTGQLLAALNPSRGVGVDISDGMVAVARKNFPTLDFRVGDI
                     EDPATFAELEGPFDVIVLSDTIGALEDFETALENIQRLCSRETRLIVSYYNRLWGPLG
                     RLAVMLGAKMPQVPQNWLSTNDIAGMLYLADFEVIKREWRQLLPKRCFGLGRLINRYL
                     GTLPILRRACLRNYVVARSTRNVAQDKRTATVVIPCRNERGNIEAAVRRLPHFCDDLE
                     ILFVEGHSQDGTLEEIRRVIAAYPERDIKVLKQDGHGKGDAVRKGFAHARGDVLMILD
                     ADLTVPPEDLPKFFKALTEGKGDLINGTRLVYAMDAFAMRPLNFIANRTFALLFSWLL
                     NERVTDTLCGTKVLTNAHYRRIAATRSYFGDFDPFGDFDLIFGAAKANLKIVEVPIRY
                     GARTYGETQISRFTHGWLLLRMVLFAHRTLKAF"
     gene            complement(8280..10229)
                     /locus_tag="VI359_09960"
     CDS             complement(8280..10229)
                     /locus_tag="VI359_09960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY6974617.1"
                     /translation="MPFLGIFEEADVPSLIEIAANADNYKRGSDRREGTLSGTTVSEY
                     ARWHDLLDGYHLSQDRQAFLAKEGLSVEASPWFLNRYGEHVLFRAITAGLNLVGKKVL
                     DVGAGAGFDSFKFVRANAQVTCVEFSPILAHDGLHKVPEARWIGGSSRVLPFGDCSFD
                     VVVANAALHHIRDIPATIGEMLRVLKPGGCLITLCDSYRKVDSGEEVEVDVFRDNVTV
                     LMGVNEGIPPLDDFLSTLVKHREQLDVRIITSEVRGLQQNPTVRKLKLPKLGKRDLLY
                     PKEWSLDEALEFLPTTSGALALLVRVKSSVQAETVQPSTSIIRPGAFARSLDNQASAT
                     AELAGHLPMQYVDLPLLDQKHKKFRLLNGWKPWVPGQSHRTAYSRARVFQTYPAGAEA
                     IRITLLAPHFEQIDRPSLEVWVNGRLIATRRLCRGLWTEYVEPVKHAVRGSVTAIEIR
                     MNTSLADERAKIFHVRELSFGHHTASRRRGEADLEQYGIEALAEIGLLRSNPARLLLS
                     HDHSLSIATLNRLRAIGFGAEIIVEKGQERFFASEPDVKVIGTYKDRFAAEDNRGRTL
                     PDDVLLIVAPEMAQVQALLSLFPASSRSRDRFAVFPGGHVRRCNSLSRSISGWRGVIG
                     ASVVMAKTLIYRVSSRLKRSTQNSW"
     gene            complement(10458..11615)
                     /locus_tag="VI359_09965"
     CDS             complement(10458..11615)
                     /locus_tag="VI359_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY6974618.1"
                     /translation="MKALAVLPPDRWMEINLLDTLRQRYCESLEVFHYPGGMGQLGSR
                     SWRVTRDRLNDELVDLARRLKSSGNLDFLFFVVYDDFLRVETASKLRALGVPMVNYHI
                     DMVFQWYRVIRTAPYFTVLAVAQMANTEHLKPYNSNIEWMPMAANPRFYRPSQAKATG
                     YQHDVSFVGSFDPYRRALLQECVNGGIKPTVFGRGWDQHSRNEYQFNWDLHKIIHDLR
                     YYGWPRWHADGLADFSAAVKRKFSRAHRLEPLKGAEIAGPCSDENMPFIFRSSRVNLG
                     FSDTGWHSQNGIVPSQKLQCRLRDFEVPMAGGFYLTQEAPDHHHYYKIGKEIDTWSEP
                     GELVEKLVFYSKNPRATERIREAGAKRALECHTWEHRFDRLFKRLDMTAQH"
     gene            complement(11646..12410)
                     /locus_tag="VI359_09970"
     CDS             complement(11646..12410)
                     /locus_tag="VI359_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY6974619.1"
                     /translation="MWNIAVIALNTFRENLRDKILYNLLLFAIFLIGGSVVLGKLSIM
                     EHEKIMTDAGLAAINMVGVVIAIFVGIGLVSKEIERRTIYTIIARPIRRLDFLFGKYL
                     GLMTTLAVNIAIMLLVYLAVLWFNHIPLHASLLQAVQLMFVEMLIVTATALCFSTFST
                     PSLSAMFTVGLFVVGHLISDLRGIAEKIDNDVIKIVITALYYICPNLEMLNIKGQAAA
                     GVSIMLSYQAIATAYGLFYSGFLLAIACLVFERRDF"
     gene            complement(12398..13357)
                     /locus_tag="VI359_09975"
     CDS             complement(12398..13357)
                     /locus_tag="VI359_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY6974620.1"
                     /translation="MKVISIEGLIKTYRSGWPGSPTVTALAGLSLSVEAGEIYGFLGP
                     NGAGKTTTLKILLGLMRANGGTAVVLGNPAGDVKTHSRIGFLPESPYFYDYLTAEEFL
                     GFYGRLAGLSHAILPQRITDMLQIVGLTDARTRQLRKFSKGMLQRIGLAQALIHDPEL
                     VILDEPMSGLDPIGRKQVRDLILSLRERGKTVFFSSHILPDVEMICDRVGIIGKGQLL
                     AEGTIDELVHRDHTDSVEVVCEGLEGEAIAGIRSVASRLIQRGRQCLIILPNPDSVAK
                     VLAEVAKHQGRLISVTPQKRSLEELFFEPAEIPCETRVPVCGT"
     gene            complement(13365..14279)
                     /locus_tag="VI359_09980"
     CDS             complement(13365..14279)
                     /locus_tag="VI359_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974621.1"
                     /translation="MIGAWKSWVLKVLVLLVLGSAVVAAVLLMEQQRPVAGRSAELSY
                     LPKGKYLKVAVLGYRHIAADLIWLKAVQYFGDMKQTAGGFRWAYHATDVVTDLDPEFM
                     IAYQAGGTILGVWSGLMDESIALLKKGMRHNPNDWHFPFLIGYDYFYELCDSANGAKY
                     FQMASTLPGAPTYLARLAARLSVEAGDPDAAMEFLARFSGQVADQRLKEALILRMKEV
                     VVERDVRQLEDAARQFQTRLGRLPLRIEELVEHKIIHALPVEPFGGRYVVLPTGAVVS
                     TELKERLRSYRKVACPSRKTAAMAVAQG"
     gene            complement(14276..16351)
                     /locus_tag="VI359_09985"
     CDS             complement(14276..16351)
                     /locus_tag="VI359_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY6974622.1"
                     /translation="MNVESFSSLASKVIRSPLEIIAPARHHELTGTSVAWFLIATAIL
                     IFSPLLEGGTTHVAVMTIRLMIFSLAGLALFRVRALDEITFMRHGLTVPISAFLLLAS
                     SSVVLSPYTHHSMQWLIVLVSYAMFLHLLVFFISRWEHVAILLGIVVSMSCAEMALTA
                     IQTKQGNIRPNGTFFNPNFLAGYLVAAWTLVLSYVSYQPIRQTKDQFFHGSKWTLLTR
                     LLAPICVAVALTGAIVLTGSRGGLLAASVGLSVVIGLRFGRRRLALFVMLFLICLAVI
                     PNPLRDRIYAEHAFNPVTYARWEMWEGALRSMVDHPMGIGLGLYQYMFPQYAFPVESE
                     IARYGTVAQTPHNEFLQMGVELGITSLLFVGWGLWILTKEIKWILRQRMTRFQRQLLV
                     GASGGIAGLLAHAAVDSNLHEPALAILLILFVAVVMAARGLLGGQLGSPMRLPLRLRP
                     ISMIGAVLILGFLAVVVVRLGVAWTWYESGTALLKKNDINGALTHYETAVAIDPGKAL
                     YHSAIAAANFHVFERHMDLAAAQVAVNHLNEAIALNPLDGRLAGLLGKVYLKVSSAPA
                     VKREERHAALLSAERAYERAIHSEPFNAVHYWEAGQVQMQLGNRQEAQSLIRHAVEIE
                     PNFLPGRDWLARVYWDKGQKSLARAEYREIVERQVRYRDWKTTPLEREFLTADANALA
                     ATLQIGGGS"
     gene            complement(16443..>16844)
                     /locus_tag="VI359_09990"
     CDS             complement(16443..>16844)
                     /locus_tag="VI359_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY6974623.1"
                     /translation="AEARTNLGAVFVSQVAFFGENNRYSDFATIGYTLAGQSNRYTYR
                     SPNAAGSAGSTGAQGADVFATLAGSATAGGVITAEPGGTTPSNATVPAAGVAGAFTAT
                     ASGNVDGDATTDHWLVNDVKAITNDLNDVTS"
BASE COUNT         4100 a         4799 c         4288 g         3657 t
ORIGIN      
        1 ggaacttggc ctcgagaaac atcaccgcat tcggctccga atagagcagt cgatgctggg
       61 gcgttttcac ttgaaatctg gccgccccat tccacataaa caggtgattg ccgaaccctt
      121 tcctacagac attcacgttg accgtgtagg caccgggcat caaattttga tccggcactt
      181 cgacacgcag gcaataacgg cctggcttca gttcatccaa ccggaaattc tgctcatagc
      241 tatccactga cacgatgaac cggtagtgcg ccgcatcaat cgtgaagcgg aaaatcggct
      301 cgtctaactt tgtaagtact tcaaggtccg cttccagggc aaatgattcg tgaaattcca
      361 gcgcatcgcg cgccgtgtca tctcccgcaa gagaacagac gcgaacattt ttaatatagg
      421 cgtccccggt tccccccgtc ggcccagccg aattctgccg ggaaagctgg tccacacgac
      481 ccgtgcgtcc atcatgagac agcgaagctg gttggaggag cgctgcgttc tgatcgtcat
      541 aggcgcgaat cacgtcctcc accgaaccag atcgtttcac ttcgccctgg tgcagaaaga
      601 gaccctggtc gcagatcgcc cagaccgctc caactgagtg cgaaaccaga ataatcgagg
      661 ttcctttagc cttcagacgg tgaaggtagt catagcattt cttctgaaaa ttgaggtctc
      721 caacggccag cacttcgtcg accaaaagga tgtcgggctc gctcatggcc gcgaccgcaa
      781 acccgaggcg aaccaacatg ccgctcgagt aatgtttcgc tggggaatcc acgaatgcgc
      841 cgacgccgga aaactcaaga atcgcttcca acctgtcatc aatctcttct ttcgtcatcc
      901 ccatgagcga gccgttcacg tagatgttct cacgacccgt taacatcggt gcaaacccgg
      961 ccccggcagc gatcaatgag cccacacggc cctgtagact gacttcaccc gcgtctgggg
     1021 gaaatacacc ggtgagaatt cgcaagagtg tggttttccc tgatccgttg acacccatga
     1081 tccccaggct ttcaccacgg gccagttcaa acgacacctc cttcagcgcc cagaattcgc
     1141 ctggacgcaa cgcaccggtg gaagtctgcc gggtagtcag tcgttgcagt gagtccttca
     1201 gtccgtaaag catggactgc cgaaggtcca tgctgaactt cttcgataag ccggtcgccc
     1261 ggattgccca atcggatcgt ttcataggcg ctctgccact ttgtcctgga tgagatagaa
     1321 cgcataaatg cccaggacca gcatgacaat ggccagggcc gaggcggcag cgtacgcgag
     1381 ggggtgcggc aacacgcccg tcatgaccag cgtgcgcggg acatcgatga gataggtcag
     1441 agggttatat tccatcatgc gttgcagagc cggcgaatcg accttgccga cgtacacaac
     1501 aggggtcacc agcatcaaca gattgaaggc cgcggtggca agggtcgtca tatctctggc
     1561 catgacgcca atgacggcaa atatcaggcc caggccgatc ccgagcgcca tgagaggcac
     1621 catcagcagc gggaagagca gaatcaacca tgatggaggg aacccgatat agatcatgat
     1681 ccccaacaac acgacgagat tagttaccag cgcgaaggca aagttaccca tcccggttaa
     1741 cgcgagcgca atcttcggaa tgttggtccg cgtgatcaat tcgccttgtg catgcaaact
     1801 gccgctcacc actgtcaata caccggtcat cattccccag aggtacgtgc cgaatagcac
     1861 gaacaccggg taggggatca ccatgtcgcc gggtttgaga atcccggcaa cgtatagaaa
     1921 cacgaatccc gccgcgttga ccatcggccc gatcaccatc cataaatagc cgagtagttt
     1981 ttgacggaac tgctgtgtga aatctcgcac aaacagtcgc catatcactt cgcgagtagc
     2041 ccaaatctcc gtcacagctt gcgccagtga ggacccgatc gtgactctca tgttcactgc
     2101 ggaataggtc acgatcggaa gatctcctgg ttgccccgct gctttattga acgatgcact
     2161 catcatgaac cacgccgaga atgaagaaac aggaagtctg cgtatcctct agactgctga
     2221 atcgacaatg cctcgacgat cgtcagagcc gtcgagattg acgtcctgct caatcgatgg
     2281 atggtatagc ccttctcaat gagtgtcctc agcagtctct cagcacttga gccgtatctc
     2341 gccagcgtct catcggacac ctcgataaag agcgatggct gaaacgttga gagagttcgc
     2401 acagctccag cgagcacctt ctcttcagcg ccctgaacat cgatcttcat gagggacacg
     2461 ggcggccaac cacgcgcctc caggaggtca tcgatcgtca gggctttgac tcggattccc
     2521 tcttccccga gcttgtggtc gactggagag aggggattca gttccagtcg tacctccccg
     2581 cttcgttcag cgacggcggc ctggacggtc tcaaccctgt cgtttaatcc agcagtggct
     2641 actattcgcc ggagtcgagc gtaattttca ggttccggct cgatcgcgat gaccttgccg
     2701 gtctgagaca cccatcgacc aaacttgacg gtaaaaaaac cgtgaaatgc accgacatcg
     2761 atgatgtgag ttccttgcac aaccattgtt gcaagcaggt tgatgtgagg agcctcgagc
     2821 agcgctttat atcctgtgta gactcggtcg aatagagaac ggccccagga cgttgaaaga
     2881 acacccgtag catggctgag gcgatagagg gacagtaatc cggactggaa tgttctattc
     2941 atgtgtgact gtttaagcgc ccagtacaaa atatacgagg taattctatc tcgaaatggc
     3001 tgtatccatt agttctttta cgatagtggt ttacgagcga ggacgcgcat attagccgat
     3061 cgtcccatca agcttggaat gagatcgacc agccagaata tggctaacgc gatgggatta
     3121 ttcaggctga aacattccgt caatgtccga ggccatcctc gatcctgcaa gacgtgattc
     3181 acggaccatg tccaaagcgc cgccgcgaca ggcgtctcgc ttcctacaac gtgaaatcct
     3241 tccttgctca acatggcgct gagagtcacc ggagtccaga gcgtccaatg gcgagggaaa
     3301 tggtaccctc cccaggtctt tcgagcgaaa aacggacggt cccagctcgc cgcatttggc
     3361 gtttcaatca gaaagatgcc tcctggacgg aggattctgt acacagagcg tacgacgttt
     3421 ctcggattag ccacatgctc aatgacttgg atcatcgtca ccacatcaaa atgggatggg
     3481 agaagatcgg cctcttcaac acggccagtg acgccctgga accctctgct gcggagcctt
     3541 tcaaccgctt gcggctcgat atcaagtccg tacaggtttt cagggtggac gccgctcgat
     3601 ctgaaggcct gcagcaacgt cccgtcgcct gctccaatat cgaggacggt gatctcatgg
     3661 ctccgtttat ccaacaaccg taagacatcc tgtatttttt gcctatcccg accctggcgc
     3721 gcttgttgta cccaggaatt cccttgttcg gcaaagctat aggagtagta cttgtcggga
     3781 gcatagatga cgttcagcat gtcaaccgtc gggcgaggat tgaggtaaaa ggtacggcag
     3841 tgatgacact gcaccagggt gaattgctgg tcaccacaac agtggttgtc atagtccggt
     3901 ccctcagccg caacgtcgta ctcgttccct gcgcaagttg ggcaaagaac ttcttcgaca
     3961 gtaatgggag cagcagaacc gttcatatgc acctttgaag tcgacgtggt ggatcagcga
     4021 ccgatggatg cctttgggag cggcctgctc tcaggtgcac gttcaatccg tagtccatat
     4081 ccaggtttca tcgccagatt ccagatcgtt ctgagatacc cattcgccca aacgatgtgc
     4141 tcacgttcga agcaaccacg ctgaaccaac aattttcgaa cctccttcag ccgatgatag
     4201 ggcactccgg gaaacaggtg atgctcaaaa tgatagtaaa acccgacagg agcgatcagc
     4261 agtcgctcaa gccatccaga ccgcaccacg cgagtcaccg cggggattct cacgagccca
     4321 acatcacaga cgtcgctgct cggttggtgc tcaacgattg ttctgaagtt accgatcaac
     4381 agtgcgacgg taaacagcgg taggacccac aagagaaagt acaaatacca tttgccatgg
     4441 attgcgcaca gcccaaacag gatgccattc cccaaaacaa atcccaccag cgatgcgacc
     4501 gaccgtttcg gaagattgga gtatcggaga tcatgttcct gcttctggta ccgctgtatc
     4561 acgtggagtc cgtagatacc ggctaaatga cgaagaatct cgagatagag attatttgag
     4621 cggatacaaa tccacgcgag cggctcgatt tcctttcggg gatcaccaag ataccggtga
     4681 tgacgcacgt gctgccaccg atactgtcca actgtcactc cggtcggacc ggaaatgaac
     4741 atgtctgaga gccaatcgtt gacccgtttg ttccgcgtga gattgtagtg agaggcttcg
     4801 tgcgtccaga gaataaaggc gttctggagg cagccgatga gaatgaacgc cgttgcaccc
     4861 acagtgaacg agcttgtcca tatcacggcg tgcagagcca gcaagacgag agcccaggat
     4921 agactgatat cggctacgac ccgtccgtac cggatggtgt gtaacgaagc gatttcatca
     4981 cggctgagcc attcattcgg ctgatctgac ggccactcct caaagagttc acttatcccc
     5041 gccaaccgtc ctgccctcgg ctcgtttgct ggcattattc ctactttcca cctgcgagtg
     5101 aatcgatccc cgacggaatc atggtgtcat caggccctag accggtttgt tcgccaggcg
     5161 attcaattcg gcagcgccag gacattcacg ccgcaaccgt caggaatcat ctgcttgagt
     5221 tcccggggat caagagaaag agggtgacct gtcgtcatgt cagccagccg atactcctgg
     5281 cacgacaaga tggtgcatag gtcctccaat gaagtgccag tctcgtcgag aacatacggc
     5341 gcgagttcca tcaccattac cggccgatct cgtttcagtg tggttgaacc gcctcgcagc
     5401 accgcacact cgtggccatc cacatcgagt ttgatgaacc cgactcgctt gattccagtt
     5461 cgctcgacat aactgtcgag ggaggtagcc tgggctcctt ccgtgtccat cagccgaccg
     5521 cagtgtttgt cgtgaacatc tgtatggccc ataagaggcc aactcgaata tagcagtgat
     5581 ggaatcctct ctccaggttt ctcgccaaac atgatttgtt cggtaatgat tcgttccacc
     5641 aattctggat tgcaggccac gtttctcacc agtttagtaa acgcgtatcg tgtcggctcg
     5701 aaccccacaa cctttccggt cgcgccgacg cttcgagcgc actgtagtgt atgcgcacca
     5761 acattggcgc cgatatcgaa cacgacgtcg cccggtttga tccagcgacg acaggccatg
     5821 accgtctccc gttcgaacat gccgaacaag taaattgcga gatcgatccc ttcttgtaga
     5881 tcgagctccc atcgaagccc atctcgaatc gtcgacactc gcgagccgag cccgacagtc
     5941 cgtcgtgcga gcatgatggc ccagtaggct gatcgtgcca gcgcaatctt cgtccgtgaa
     6001 cgtatcatcc aagccatgac gtgcccggcc gcactactcc gctcgttgca caatcaccaa
     6061 cgctctaaat gccatgactg aaccaagaag tggcaggagc acctcctcca tgtggagaag
     6121 cggctccacc aaccagctgg ggagcaagct ccacgatcgg aatcctcctg acaatggata
     6181 aacaaagagg cttaaccatt tgatggtcct gacacgcaaa gatcgaaatt cgtgttcaag
     6241 cctctgccgg tttctcagaa acaacaacgt tggaatcgct tgattcccgt cgtaaggatt
     6301 ttggttcgcc cgcagtactg accgggccaa cgggtcatcg gctaactgca ccggttccgg
     6361 atgaaaacac cgatagaaca accaactgag cggagtaatg gctggctcca atgcgatgag
     6421 aaacccgcct ggtctcagga gtcgttcagc ttctgccaga aagagtcgcg ggcaaggaag
     6481 gtgatgcaga acgtcaaaca tcacgatgtt atcgaaactc cgatctctaa atggcaacgc
     6541 ctgcgcgtcg gcgacggcgt ccagccaagt cgcaggcaag atgtcggtcg agatgacatg
     6601 tgaggcaaat gctttgaggt ttcctgatcc tcctccaatc tccagcgtca gacctggcct
     6661 gcacgcagca ataatttgtc tgtaatagtc ttgatacaga gcgcggagga ccggtttgct
     6721 ctcccaaacg gtgcggtagt ggcggagcct ggaatccgta cccatctaga acgctttaag
     6781 tgtgcggtgg gcaaacaaga ccatgcgtag cagcagccac ccgtgagtaa atcgggaaat
     6841 ctgtgtttct ccataagtgc gcgcgccata ccgaatcggc acttccacga tcttcagatt
     6901 ggccttcgca gcgccgaaaa tgagatcaaa gtctccgaag ggatcaaaat cgccaaaata
     6961 agatcgtgtc gcagcaatcc gacggtaatg cgcgttcgta agaacctttg tcccgcagag
     7021 tgtatcggtc acccgttcat tgagtaacca ggagaaaagt aatgcaaatg tcctattcgc
     7081 gatgaagttg agcggcctca ttgcaaaggc gtccatggca tataccagtc tcgtcccatt
     7141 tataagatcg cccttgcctt cagtgagggc tttgaagaac tttggcaggt cttctggagg
     7201 caccgttaga tccgcgtcca ggatcatcaa gacatcccct cttgcgtgcg cgaacccttt
     7261 tcgcaccgca tcacctttcc catgtccgtc ttgcttcagg actttaatat cgcgctcggg
     7321 ataggccgca atgacccggc ggatctcttc taacgtgcca tcctgactgt gcccctctac
     7381 aaacagaatt tccagatcgt cacagaagtg cgggagccgt cgaactgcag cttcaatgtt
     7441 gccgcgctcg ttccgacatg gaataacgac agtagcggtt ctcttgtcct gcgccacgtt
     7501 tcgtgtcgac cgcgcgacga cgtagtttcg caaacaggca cgccgcaaga tcggcagagt
     7561 cccgaggtac cggttgatca ggcgcccaag accaaagcag cgtttcggta gtaactgccg
     7621 ccattcacgc ttgatcactt caaaatctgc cagatacagc atgccggcaa tatcgttcgt
     7681 cgaaagccag ttctgcggca cctggggcat tttggctccc agcatgacgg cgagccgtcc
     7741 gagcggtccc cagagtcggt tgtaatagga cacgatcagc ctcgtctcgc gagaacagag
     7801 ccgttggata ttttccaacg ccgtttcaaa atcttcgagt gcgccaatgg tatctgacag
     7861 cacaatgaca tcgaacggcc cctcgagttc agcgaaggtc gccggatcct cgatatcccc
     7921 tactctgaag tccaaggtag gaaaattttt ccgtgccacg gcgaccattc cgtcactgat
     7981 gtcgacaccg actccccgcg agggattgag tgccgccagt aattggcctg tcccgcaacc
     8041 caaatcgagg acacgctgac ccggcggaat aagaaactgc atgtaccgcc gatcatcttc
     8101 ataataaaag gcgtttttct cgatccagcg atcacgcgct gacgccagtt cgtctgaaag
     8161 ctgacgaacc gccgtcttgc gggaagatac gagaagctga tcacaaactg ccagcataga
     8221 cgagcctata gaacggatgc gattccttcg agagtcggcc gtgaggccat tcgatctcct
     8281 caccatgagt tctgagtcga gcgtttcaga cggctcgaca cgcgatagat caacgtcttg
     8341 gccatcacga cgctggctcc gataacgcct ctccagccag aaatcgaacg ggacagcgaa
     8401 ttgcacctgc gaacatgccc gccgggaaaa acagcaaaac gatcacggct ccttgaactg
     8461 gccggaaaca aggaaagcag cgcttggact tgtgccatct ccggagccac gataagcagg
     8521 acatcatccg gaagagttct cccccggtta tcctcggcag cgaaccgatc tttatacgtc
     8581 ccgatcacct tcacgtctgg ctcggacgca aagaatcgtt cctgtccctt ttcaacaatg
     8641 atttctgctc caaaaccgat ggccctcaga cgattgagcg tggcaatcga aagactatga
     8701 tcgtgcgaca agagcaggcg cgctggattg gacctgagca gacctatttc tgccaacgcc
     8761 tcgatcccgt actgctcaag atccgcctca cctctacgtc ggctagccgt gtgatgtcca
     8821 aaactcagtt cgcgcacgtg gaagatcttg gcccgctcat cggcgagcga tgtattcatc
     8881 cgtatctcga tcgctgttac ggaccccctc acggcgtgtt tgacaggttc gacatattca
     8941 gtccaaagcc ctcgacatag tcttcgtgtc gcaatcaacc ggccattcac ccacacttcg
     9001 aggcttgggc gatcgatctg ttcaaagtga ggagcaagga gggtgatgcg gatggcttct
     9061 gctcctgcag gatatgtctg aaatacacga gcccggctat acgctgttcg atgggactgc
     9121 cccggtaccc aaggcttcca gccattcagc aaccgaaact tcttatgctt ctgatcgaga
     9181 agtggaagat caacgtactg catcgggaga tggccagcca gttcagccgt ggcactggcc
     9241 tgattgtcca gtgaacgggc aaatgcacca gggcgaatga tacttgtaga cggctgaact
     9301 gtttctgctt gaacggaact cttgacccgt acgaggagtg caagcgcgcc ggacgttgtc
     9361 ggaaggaatt ccagcgcctc atcgagggac cattccttcg gatacagcaa atcgcgcttc
     9421 ccgagtttcg gtagctttaa ctttctgacc gtcggatttt gctgcagccc acgaacttca
     9481 ctcgtaataa tacgaacgtc gagttgttct cggtgcttga ccaacgtgga cagaaaatca
     9541 tccaaaggtg gaataccttc attcacaccc atcaaaacag tgacgttatc tctgaataca
     9601 tcgacttcga cctcttcgcc ggaatcgacc tttcgatatg aatcgcagag cgtgatcaag
     9661 caccctccag gcttcaacac tctgagcatt tccccaatgg tggcgggaat atctctaatg
     9721 tggtgcaacg ctgcgttcgc cactaccaca tcaaagctac aatcgccaaa cggcagtact
     9781 cttgagctac ctccaatcca acgtgcttct ggaaccttgt gtaacccgtc atgggcaagg
     9841 atcgggctga attcaacaca ggtgacctgt gcattggccc gtacgaactt aaagctgtcg
     9901 aatcccgccc ctgctccgac atcgagaact ttcttgccca cgagattcaa tccggcggta
     9961 atggcccgga agagcacatg ctctccatat cgattcagga accagggaga agcttcgact
    10021 gacagtcctt ctttcgcgag gaacgcttgt cggtcttggc ttaagtgata tccatccagt
    10081 aagtcatgcc accgggcata ctccgacact gtggtccctg acagcgttcc ctcgcgtcga
    10141 tcactgccgc gtttatagtt atcggcgttg gcggcgatct caatcaggct cgggacgtcg
    10201 gcttcttcga aaataccgag gaatggcact ccccagacta gatcgaatct ttggcaacac
    10261 gcactgcaga caatttcgtt gtcgtggatc tgcgccgcat cagaatcgcc taggcaggac
    10321 agtccaagcg cagacgactt acagaatgga cactgacagt catgtagatc gggcagactc
    10381 atggtgcttc aatttcttgt agcgtgattc gaggttgttt ccggtttctg ccacaattat
    10441 ctagaacgca gacgcgatca atgctgagcg gtcatgtcta gtctcttgaa taggcgatcg
    10501 aagcgatgtt cccatgtgtg gcattccaga gcccgttttg ccccggcttc acgtatgcgt
    10561 tcggtcgcgc gtgggttttt ggaatagaac acgagctttt ccacgagctc gccgggttct
    10621 gaccaggtgt cgatctcttt gccaatcttg taatagtgat gatgatccgg cgcttcttgg
    10681 gtcagataga aaccaccagc catgggcact tcgaaatcac gcaggcggca ctgaagcttc
    10741 tgcgaaggaa caatcccgtt ctgactgtgc cagccggtgt cagaaaaacc caaattgact
    10801 cggctcgatc ggaatatgaa cggcatgttc tcatcggaac acggccctgc gatttcagcg
    10861 ccttttagcg gctctaaccg atgggcccga gagaatttcc gcttcactgc cgcactgaaa
    10921 tcagccagac cgtctgcatg ccatcgtggc caaccgtaat atctgagatc atggatgatt
    10981 ttgtgtagat cccagttgaa ttgatactca ttacgactat gctgatccca cccacggccg
    11041 aagacggtcg gcttgatacc accattcacg cactcttgga gaagagctcg gcgatagggg
    11101 tcgaaactgc cgacgaacga cacatcgtgc tgatacccag tcgccttcgc ctgggatggg
    11161 cgatagaacc tcgggttggc tgccatgggc atccattcga tgttggaatt gtagggcttg
    11221 agatgctccg tattcgccat ttgcgctacg gccaacactg tgaagtacgg ggcagtccta
    11281 ataacccgat accactggaa caccatgtca atgtgatagt tgaccattgg tacgccgagc
    11341 gcgcgcaatt tcgaagctgt ctcgactcga agaaaatcgt catagacgac gaagaacagg
    11401 aagtcgagat tcccggatga cttgagacgg cgtgccaggt ccaccaactc atcgttcaat
    11461 cgatctcgcg tcacccgcca cgacctggat cccagttgtc ccattccacc cgggtagtga
    11521 aacacctcaa gcgactcgca gtatcgctgc ctcaacgtgt cgaggagatt aatctccatc
    11581 caccgatctg ggggaagcac ggcaagcgct ttcatgatct gtcgatcatc taccgaaccc
    11641 tccggttaaa aatcgcgccg ctcgaagacg aggcaggcga tggcgagtag aaatcctgag
    11701 tagaacagtc catacgccgt ggcaatagct tggtatgaca gcatgattga cacgccagca
    11761 gctgcctgcc ccttgatgtt gagcatttcg aggttcggac aaatgtaata cagggctgta
    11821 atgacgatct ttatcacgtc gttgtcgatt ttctcggcaa tcccccggag gtctgagatc
    11881 aggtggccga caacaaataa tccaacggtg aacatggcgc tcagcgaagg agtgctgaag
    11941 gtcgagaagc agagcgctgt cgcagtcacg atgagcattt cgacgaacat caattgcaca
    12001 gcttgaagca aagaggcgtg cagaggaatg tgattgaacc aaagcaccgc cagataaacc
    12061 agcagcataa tggcgatatt cactgcaagc gtcgtcatca accccaagta ttttccaaaa
    12121 agaaaatcga gcctccgaat aggtctcgcg atgatggtgt agatcgttcg ccgttcaatc
    12181 tccttgctca caagtccaat cccgacaaag atcgcaatta cgacgccgac catattgatc
    12241 gccgccaatc ccgcatcggt catgattttt tcgtgctcca tgatggaaag cttgcctaag
    12301 accactgagc caccaatcag gaagatcgcg aacaggagca ggttatagag aatcttgtcc
    12361 cggagattct ctcgaaaggt attcagggca atgacagcta tgttccacat actggcaccc
    12421 gtgtctcgca gggaatttct gcaggttcaa aaaatagttc ttcgagagat ctcttctgcg
    12481 gcgtaacaga aatcagccgc ccttggtgct tggccacctc ggccagaacc ttcgcaacag
    12541 aatccggatt cgggagaata atcagacact gccgcccccg ttggatgagg cggcttgcga
    12601 cggaccgaat cccggcaatt gcttctccct caagtccctc gcatacaact tccactgagt
    12661 ccgtatgatc acgatgaacc agctcatcaa tcgttccctc ggccaacaat tgtcccttgc
    12721 caataatccc gacgcggtcg cagatcatct cgacatcggg aaggatatga gaactgaaga
    12781 acacggtctt gccgcgctcg cgaagactca ggattagatc acgcacttgt ttgcgtccga
    12841 tgggatctaa gcccgacatc ggctcgtcaa gaatcaccag ctcagggtcg tggattagcg
    12901 cctgtgccag accaattctc tggagcatcc ctttggagaa cttacggagc tgtcgagttc
    12961 tcgcgtcggt caagccaaca atttgaagca tgtctgtaat ccgttggggc aggattgcgt
    13021 gacttaatcc tgcaagacgt ccgtagaatc caaggaattc ttcggccgtt agataatcat
    13081 agaaatacgg agactcggga agaaacccaa ttcggctgtg ggttttcaca tcaccagccg
    13141 gatttcccaa taccacggca gttccaccgt tcgcccgcat caagccgagt aagattttga
    13201 gtgtggtcgt cttccccgcg ccatttgggc ctaagaagcc ataaatttct ccggcttcga
    13261 ctgagagcga caatcccgca agggctgtga ctgtggggct acctggccat ccggatcgat
    13321 acgtcttgat caggccttcg attgaaataa ctttcatgtg ttggttaccc ttgagcgact
    13381 gccatagcag ctgttttcct gcttgggcaa gccacttttc tgtacgagcg aagccgttcc
    13441 tttagctccg tgctgaccac tgccccggtt ggaagtacga catagcgccc cccaaatggc
    13501 tcgaccggaa gagcatgaat gatcttgtgc tctactaact cttcaatccg gagcggcagt
    13561 cgaccaaggc gggtctgaaa ttgacgagcg gcgtcttcga gttgtcgaac atcacgttcg
    13621 accacaacct ctttcatccg tagaatgagg gcctctttaa gtcgctgatc tgctacctgg
    13681 ccactgaatc tcgccaaaaa ctccatcgca gcatccgggt ccccggcctc gacagaaagg
    13741 cgagcggcaa gtcgggcgag ataggtcggc gcgcctggaa gcgtcgacgc catctggaaa
    13801 tatttggctc catttgccga atcacagagt tcatagaaat agtcgtaacc gatcagaaat
    13861 gggaaatgcc aatcgttcgg gttatgcctc attcccttct tcaacaaggc tatgctttcg
    13921 tccatcaagc ccgaccagac accgaggatc gtccctcccg cctgataagc aatcatgaac
    13981 tccggatcca aatctgtgac gacatcggtt gcgtgatatg cccaacgaaa tcctccagcc
    14041 gtctgcttca tgtcaccaaa atactgtacg gccttgagcc aaatgaggtc tgcagcaatg
    14101 tgacggtacc ccaacaccgc caccttcagg tacttgccct tcggaagata tgacaattca
    14161 gcagaacgac ccgcaacagg acgctgctgc tccatcagca gaacggcagc gacaacggca
    14221 ctgccgagga cgagcagcac gaggactttt aagacccaag acttccatgc cccgatcaac
    14281 ttccacctcc aatttgcaat gttgctgcta gcgcgttcgc atcagccgtc agaaattccc
    14341 tttccaacgg agttgttttc caatcccgat agcgaacctg gcgttcgacg atctctcggt
    14401 attctgctcg agctaggctt ttctgtcctt tatcccaata cactcttgca agccagtcgc
    14461 gtccaggaag aaagtttggc tcaatttcga ccgcgtgtct gatcaacgac tgcgcctcct
    14521 gacggtttcc caattgcatc tggacctggc ccgcctccca ataatgaacg gcgttaaaag
    14581 gctcggaatg aatcgcccgt tcatacgccc tttcagcact taaaagagcc gcgtgcctct
    14641 cttctcgctt caccgcagga gctgacgaga cttttagata gacttttcca aggaggcctg
    14701 ccagtcgtcc atccaacgga ttcaacgcga tggcttcatt caagtgattc acagccacct
    14761 gggccgcagc gagatccatg tggcgctcga acacatgaaa attggcagca gcgatcgcgc
    14821 tgtgatagag cgctttgcct gggtcgatgg caaccgccgt ttcgtaatga gtcagtgcgc
    14881 cattgatatc gtttttcttc agcaatgcgg ttcccgattc ataccacgtc caggccacac
    14941 ccagccgaac gacgactaca gccaagaacc ccagaatgag tactgctccg atcatcgaaa
    15001 tcgggcggag cctgagagga agacgcattg ggctgcctag ctgaccacca agcaaccctc
    15061 tggcggccat tacaacagcc acgaacagta tcagtagaat ggctaaggcc ggctcatgga
    15121 ggttcgaatc aaccgctgca tgtgcgagta gccctgcgat tccacccgat gccccaacca
    15181 aaagctgtcg ctggaacctt gtcattcgtt gcctcagaat ccatttgatc tccttcgtga
    15241 gaatccacag gccccatcca acaaaaagca aactcgtgat gccgagttcg acacccattt
    15301 gcaagaactc gttatgggga gtttgggcga cggtgccata tctggcaatt tctgattcga
    15361 ccgggaaggc atactgtgga aacatatatt ggtacaatcc gagcccaatc cccatcgggt
    15421 gatcaaccat acttcgcaaa gccccttccc acatttccca acgagcatag gtgaccggat
    15481 tgaaggcatg ctctgcatag attcggtcac gtaagggatt ggggattact gcaaggcaga
    15541 ttagaaagag cataacgaat agcgcgagcc tacgacggcc gaatcgcaat ccaataacga
    15601 ctgataatcc tacgcttgct gccaatagcc caccgcgaga ccctgtcaaa acaatggccc
    15661 cggtcagcgc taccgccaca caaattggcg caaggagtct tgtcagcaga gtccacttcg
    15721 atccatgaaa gaactggtct ttcgtttgac ggatcggttg gtaggaaacg taggaaagaa
    15781 ctagcgtcca tgctgccacc aaatagccgg cgaggaaatt tgggttaaag aatgtcccat
    15841 tcggccggat gttcccctgc ttcgtctgga tagcggtcag cgccatttcc gcacaactca
    15901 tagacacgac gattcccaac aagatggcca catgctccca cctagatatg aaaaagacaa
    15961 gcaaatgcag gaacatggcg taactcacca agacgatcag ccactgcata ctatgatgcg
    16021 tgtatggtga gagcacgaca gaagaggaag ccaacaacag aaaggccgag attggcacgg
    16081 tcagtccatg ccgcatgaag gtaatctcgt cgagcgcacg cacgcggaag agagctagtc
    16141 cggctaaact aaagatcatc agccggatcg tcataacagc gacgtgagtg gttccgccct
    16201 ccaagagagg agaaaaaatg aggatggccg tagcgatcag aaaccaggca acagacgttc
    16261 cggtcaactc atgatgacga gcaggcgcga taatctctag gggtgagcgg atgactttcg
    16321 atgcgagaga agaaaaggac tctacattca cggctctatc aatcgctttg gtagaggaga
    16381 aaaaacaagg cggggagagc gatgctcccc ccgccacagg gacactactg actgaggacc
    16441 acctagctgg tcacatcgtt caggtcgttg gtgatcgcct tgacgtcgtt caccaaccag
    16501 tggtctgtgg tggcatcgcc atccacattt cctgatgccg tcgccgtgaa ggccccggcc
    16561 acaccggcgg caggcaccgt ggcattcgac ggcgtcgtgc cgcctggctc cgctgtaatc
    16621 acaccaccgg cagtcgcgga cccggcaagc gttgcgaata cgtccgcgcc ctgcgcacca
    16681 gtgctccccg cgctgccagc ggcgttcgga ctccggtagg tgtaccggtt cgattgaccg
    16741 gcgagggtat agccgatcgt cgcaaaatcc gagtaccgat tgttttcacc gaagaaggcg
    16801 acttgcgaca caaacaccgc gcccaggttg gtcctggctt cagc
//
LOCUS       DATDNW010000415         9721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospiraceae bacterium isolate SMAG_U447
            k141_955552, whole genome shotgun sequence.
ACCESSION   DATDNW010000415 DATDNW010000000
VERSION     DATDNW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962647
            Sequence Read Archive: SRR25158214
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospiraceae bacterium (soil metagenome)
  ORGANISM  Nitrospiraceae bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 21:18:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,009
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,975
            CDSs (with protein)               :: 1,975
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Nitrospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955552"
                     /isolate="SMAG_U447"
                     /isolation_source="soil"
                     /db_xref="taxon:2026770"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.16 N 119.39 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VI359_09995"
     CDS             <1..418
                     /locus_tag="VI359_09995"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEY6974624.1"
                     /translation="MKFGGMYREVQKQNDHFVLSYDRAWYQVWKQSQNPFPADEPIYC
                     FTAVFAPVALDTTVFHHWYYRANSSKPFAHADRIPIKIAGGREGGYRAYTFKQRLDPG
                     DWRVDVETEDGRVIGRVSVVVEERGDAQPTPRTLSY"
     gene            418..837
                     /locus_tag="VI359_10000"
     CDS             418..837
                     /locus_tag="VI359_10000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY6974625.1"
                     /translation="MKQLSGLKLLEEREGEGQPAQKGDRVVYNTRIFLNRGDEVPLNE
                     IQAEHLPTQMVRVDDRTTFIDHTIVLGRRQAMAGVEHALLGMKVGGYRKVRVSPHLAY
                     RDKGIPDLIPPEALLICEIWLRDIVRSSSRGSSLEVK"
     gene            834..1196
                     /locus_tag="VI359_10005"
     CDS             834..1196
                     /locus_tag="VI359_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY6974626.1"
                     /translation="MNPRTLASCPSSPNCVSTHAQDAGHAIAPYRYRKSRAEAKEALK
                     NIVHSLPRTKLVEEDADYLRYEFTSLIIRFVDDVEFIFDDETKTVHFRSASRTGYGDF
                     GVNRRRMEEIRSLAEGKL"
     gene            1502..2233
                     /locus_tag="VI359_10010"
     CDS             1502..2233
                     /locus_tag="VI359_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY6974627.1"
                     /translation="MGILTYTATVSRVQDLTHDVRELEITLVEPPTISFKPGQFISFE
                     VPRIGFPLPVVRPYSIASPPSQDNRVTLLFNLVTDGPGSTYLYSLRVGDHTTFKGAAG
                     SFWLKDDARDLLFVATGTGIAPIRSMVAAQLERGTTQAVTLFWGLRSQRDLYYQEELF
                     ALTRQFPKFSFVTTLSRPEAGWTGATGRVTTLVRDRITSVRNLAVYLCGNSGMIQEVT
                     TLVQARGLCPIHKEKYYDDRGEPTD"
     gene            2604..3044
                     /locus_tag="VI359_10015"
     CDS             2604..3044
                     /locus_tag="VI359_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEY6974628.1"
                     /translation="MSISSGPESTILPTNVPTTSSIPIRDMVGAGKAWGLCTAVDLQD
                     CNPDLIRDPEQITRYVIELCELIGMQRFGECQVVNFGEGRVAGYSMVQLISTSLISGH
                     FANDTNSAYLDIFSCKGYDPATVEAFSKEFFGARRSTATATLRY"
     gene            3066..3461
                     /locus_tag="VI359_10020"
     CDS             3066..3461
                     /locus_tag="VI359_10020"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY6974629.1"
                     /translation="MKGPRLRGPRCVLRRIAFLPHMKPKTVKEFFQSLPAKLDRKAAE
                     DLDAVYQFDLSGPQGGQYLLRIQDGICTVEEGTHTDPHVTLAMAGEDCIKVLNGQMSG
                     PAIAMSGRLQISGDIGLAMQLKSLFPTVG"
     gene            complement(3473..6664)
                     /locus_tag="VI359_10025"
     CDS             complement(3473..6664)
                     /locus_tag="VI359_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY6974630.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVVLGATSLQRLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGGVQIWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVSFVTVSSDDLDERALYDLAFNT
                     IAPQIEQIANVAAATVEGGKIRQININLDPALLNARGLSILDVVKAVKESNLILPSGD
                     IKAGNLDYNVFTNNQFRTVDPIQDVIVKVNQQGNPVRVRDVGTVTDSSDIQTNVVRTD
                     GKRAVYLRVNKQPIANTVEVVDALRKALPKMVGIPPGVKLGISFDQSVYIRQSIKNLI
                     EQALHGSLLAAAVILIFLRNLTSTLIISVAIPLSIMVTFIVLYFTGQTLNVFTLGGLA
                     LGIGRLVDDSIVELENIQRHLNTTPRRWEAILEAAREVAMPIFASTVTTVVVFLPIFF
                     VVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCYKFLKPEREAHRSMPAWFVRLMN
                     WSRERYEWLDKGYEDSLRWVLAHRRLFIMGIVLIFAASLMLLPMIGTEFLPVSDESQF
                     RIVLRAPVGQRVEKTEQQVAEVERVLRENIPPHELETIVSSTGVLAQGRSSLFNPNTG
                     PHTSVISVYLASPDKRTRNQVEIMNEVRPKAIKLFPGVAMFFDPGGLVKRVTSFGSQK
                     AVDVEIYGYDFEHARTVIRQVENIMHQVQGLADIEVSREENYPEVNVVVDREKAALLG
                     ISETDVANAVLFSLNGNGQTDPIIYTDPQNGNEYYISAWLAEEHRKDLTDIENIILTA
                     RNGEPVLLKNVASLKLNAGPVKIDRKYFQRVIHITANPVNRDLGAIAADLEEEFAKLQ
                     LPTGFSIRLAGQIQQQRETFEGLFFATILALMLVYMVMAAQFKSLIDPFIIMFSVPMG
                     FPGVILVLFLTNTTLSTTSMMGIIMMLGIVVSNGVLLVDYTNVLRRKGQALREAVVVA
                     ARTRLRPILMTSLATVFGLLPMAIGLGTGGETNAPLARAVVGGLSVSTLLTLFLVPTM
                     YMILEERFPRQLREEQSEPAVLPVQTQGEPVFR"
     gene            complement(6672..7901)
                     /locus_tag="VI359_10030"
     CDS             complement(6672..7901)
                     /locus_tag="VI359_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY6974631.1"
                     /translation="MNPLKQHPIVTLGVIIFFAVTALVVFRLSSGAKTDTRKARVITV
                     GTVTPLKQDLDVRLAYTADITPNQLVNLFSRVDGYIAKIYVDKGDFVKANQLLVEIDH
                     TDYVHAVNQAKANLASARAKVAQQEANVRNAKLTLDRMQALIKDQFVSQQDLDNAQVS
                     FDAAIAAQDSLRAQVKQMEVALAQAETNLAYSYIRAPFAGYIAERNLDPGSYVSGATA
                     STSTMSRGILSLHDVETVRILIEVVEKNVPLVQIGQKAEVRAEAYPDRVFEGTVTRIV
                     QALNRATRTMTVEVDLPNKNHMLKGGMFARVEVLVGKHARAVQIPIDAVSRLEDLQYV
                     YIVRDGQAQRVEVEIGLRDENRVEITKGLTGSEQVIVSGKDLVHDGTPVQTQPLLSDP
                     APAPANTAPPASPPAKG"
     gene            complement(7898..8533)
                     /locus_tag="VI359_10035"
     CDS             complement(7898..8533)
                     /locus_tag="VI359_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY6974632.1"
                     /translation="MPSRPIRKTMRVSSQERQASLIASAASLFAAKGFNGTTTKEIAN
                     AAGISEALVFKYFPTKRALYAAILAEKVTVSELLEAVEEATKKRDDQRVFTLIASYRI
                     RPGVDPTLLRLLLFSALEGHELSDMFFGKQHKVFYEHLATYIQTRIQERAFRKVDPLL
                     AARAFIGMVVHHRLLHEIFGVPMHRSHEETVATYVDLFLNGLKQPSLGGAA"
     gene            complement(8712..9092)
                     /locus_tag="VI359_10040"
     CDS             complement(8712..9092)
                     /locus_tag="VI359_10040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974633.1"
                     /translation="MKSLRVRTHKSSHQFTAPCNRREADRASVDFGLMYSAQDARGEL
                     IMGDGMVTNLSQKGLGIRGDTAVTPGTELTLFLYLPDGRDPLFVMEARVMWAAGHQFG
                     VRLLKMNVRELNRLHSFLRANSPL"
     gene            complement(9147..9524)
                     /locus_tag="VI359_10045"
     CDS             complement(9147..9524)
                     /locus_tag="VI359_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY6974634.1"
                     /translation="MRDRILETADNAPALINPDDRRRIMRVPAQFGLMYSGMDSGHML
                     IGDGMVTNLSEGGIGICGNRLVKPGMELALFVDLPGAEDPVCLSEGRVSWVAGRKFGV
                     QLLTVPVDAQHQLRSFLWGHHSP"
BASE COUNT         2100 a         2719 c         2836 g         2066 t
ORIGIN      
        1 gatgaagttc ggcgggatgt atcgggaagt gcaaaagcag aacgaccact ttgtgctcag
       61 ttacgaccgc gcgtggtacc aggtgtggaa acagtcgcag aacccctttc cagccgacga
      121 acccatctat tgctttaccg ctgtcttcgc accggttgca ctggacacga ccgtgtttca
      181 tcattggtac taccgcgcga acagcagcaa gcccttcgcg catgccgaca gaattcccat
      241 caagatcgcc ggcggccgcg agggtggata ccgggcctat acgttcaagc agcgactcga
      301 tcccggcgac tggcgcgtgg atgtcgagac tgaagatgga cgcgtgatcg gacgggtttc
      361 ggtagtggtg gaagagcgag gagacgcaca gccgactcca cggacgctct cgtactaatg
      421 aaacagctca gtggactcaa gctgcttgaa gagcgagaag gcgaggggca accggcgcag
      481 aaaggcgacc gagtcgtcta caacaccaga atctttctca accgcgggga cgaggtgccg
      541 ctcaatgaga ttcaagcgga gcaccttccc acacagatgg tgcgagttga cgaccggacg
      601 acattcattg accacaccat cgtactggga cgccgtcagg ctatggccgg cgtggaacat
      661 gcgttgttgg gcatgaaagt cggcggctac cgaaaggtcc gtgtcagtcc gcatctcgcc
      721 tatcgcgaca aaggcatccc ggatttgatt cctcccgagg ccttgttgat ttgtgagatc
      781 tggttgcgcg acattgtgcg atcttcttcg cgcggatcgt cactcgaggt caaatgaacc
      841 caagaacact ggcctcttgc ccttccagcc ctaactgcgt gtcgacccac gcgcaagatg
      901 ccggccacgc catcgcgccg tatcggtatc gaaagtcgag agctgaggcg aaagaggcgt
      961 tgaaaaatat tgtccactcg ctaccgcgca ccaagctcgt cgaagaggat gccgactacc
     1021 tccgctatga gttcaccagc ctgatcatcc gtttcgtaga cgatgtggag tttatcttcg
     1081 atgatgagac caagacggtt cactttcgct ctgcctcccg aactggctat ggcgatttcg
     1141 gtgtgaaccg gcgccggatg gaagagatca ggtcgctggc tgaaggaaaa ttataaggcg
     1201 tggcaggcgg cggtcggagt gagctcggcg tcgttgaatt cgacattcgc gaggtaggcc
     1261 cagcccatca agtaacccag ttggcgatag gcttcgaatt cctccaattc gtaccactgc
     1321 gagcttgtgc tggtctgtga taacccttct ctcttgggcg actctacgaa cttgctgaga
     1381 gcggaaacca caggcagaga ggaagaagaa gccagtcggt agctggaaga gaggccgatc
     1441 ttcttgatac cgtgtccaga gagcggcggt tcgtgatcat tccaacgtgg ttgaaggatc
     1501 gatgggcatc ctcacataca ctgcaacagt cagtcgagtt caagacctga ctcacgacgt
     1561 tcgtgaactc gagatcacgc tggtcgaacc gccgaccatc tcattcaagc ccggtcaatt
     1621 tatctcattc gaagttccga ggatagggtt ccctctaccg gtcgtgaggc cctattcgat
     1681 cgcctcacca ccctcccagg ataatcgggt taccctcctg ttcaatctgg tgacggacgg
     1741 tcccggctca acctatctgt atagtctccg agtcggcgat cacaccacgt tcaagggagc
     1801 ggctgggtcc ttctggttga aagacgacgc gcgggatctc ctgtttgtcg ccacaggaac
     1861 gggcattgcg ccgattcgat ccatggtcgc agcccaactt gagcgaggga caacgcaagc
     1921 cgtgacattg ttctggggcc tgcggagcca gcgagacttg tactatcagg aggaattgtt
     1981 tgcgctaacc cggcagttcc ccaagttctc ctttgtcacg accctgtcgc gcccagaagc
     2041 gggatggacc ggtgcgacag gacgggtgac cactctcgtg cgtgaccgaa tcacatcagt
     2101 ccgcaacctg gccgtgtatc tctgcggtaa cagcggcatg attcaggagg tcacgacact
     2161 cgtccaggct cgcggcctct gtcctattca caaggaaaaa tactacgacg accggggtga
     2221 accgacagat tagtgcgccg gttgcgccgg ggttgggccg agcctgatcg tatgctgaaa
     2281 aatgcatttc gggcaggtgt agtggagaaa ctggccgctc gcgacgagcc gtttctgcat
     2341 gggcaccttg caccaggtac aaaggcggtc tggaagatcg gccattgagg ggaccgaagg
     2401 tacgtgagga gacgtcgcgt cggccatgcg aggcattgtc cccgagcgtc gagagccttg
     2461 tcaaccgagt gccatgtctt cggttggccc ggactaaaaa aagtacggcc gtcatgcatc
     2521 attgcaaaat aatcgtggta tagtggatac catgttcacg cagacagatt tctcaccctc
     2581 acacagaaag gtgcccaacc tctatgagca tctcatccgg tcccgagtcc accatcttac
     2641 cgactaacgt cccaaccacc tcgtcgattc cgatccgcga tatggtcgga gccggcaagg
     2701 cgtggggact gtgcactgct gttgacctcc aagattgcaa tcccgatctc attcgcgacc
     2761 ctgagcagat cacgcgctac gtgatcgagc tctgtgagct gattggaatg cagcgtttcg
     2821 gtgagtgcca ggtggtcaat ttcggcgaag gccgcgtcgc cgggtattcc atggtgcagc
     2881 tgatttcaac gtcgttgatc agcggccact tcgccaacga tacgaacagt gcctacctcg
     2941 atattttcag ctgcaaaggc tatgacccag cgaccgtgga agccttttcg aaggaattct
     3001 tcggtgcgcg tcgcagtacg gcgactgcca cgctgcggta ctaaccgtgt ctcgttgaac
     3061 ttcgtgtgaa ggggccgcgg ttgcgcggcc cccgctgtgt cctccggcgg attgcgttcc
     3121 tgccccacat gaaacccaaa accgtcaaag aattctttca gtcgttacca gccaagctcg
     3181 acaggaaggc tgcggaagac ctcgatgcgg tctatcagtt tgatctcagc gggccgcagg
     3241 gggggcagta tctcctccgg atccaggacg ggatctgcac cgtagaagaa ggaacccaca
     3301 ccgatcctca tgtcacgctc gccatggccg gcgaggattg catcaaggtc ttgaacggtc
     3361 agatgagcgg ccctgcaatt gcgatgtcgg gccgccttca gatcagcggg gacatcgggc
     3421 tggcgatgca gttgaagtct ttgtttccaa ccgtaggcta gcacttcatt cctcagcgaa
     3481 agaccggttc cccttgcgtc tgcacaggca gtacggctgg ctctgattgc tcttctcgga
     3541 gttgccgcgg gaaccgttcc tccaggatca tgtacatcgt cgggacaaga aagagggtaa
     3601 gcagggtaga gacgctgagt cctcccacca cggcgcgggc gagcggagca ttcgtttcac
     3661 cccccgtgcc caaaccgatg gccatcggaa gcagcccgaa gaccgttgcc aacgaggtca
     3721 tgagaatggg gcgcaatcgc gtccgggccg ccaccacaac cgcctcacgt aaggcctgcc
     3781 ctttccgtcg caacacattc gtgtaatcga ccagcagcac gccattcgag accacaatgc
     3841 cgagcatcat gatgataccc atcatagagg tggtggagag cgtcgtgttc gtgaggaaca
     3901 gcaccagaat cacaccggga aaccccatcg ggacagaaaa catgatgatg aaaggatcga
     3961 tgagcgattt gaactgagcg gccatgacca tatacaccag cattaacgcc aggatggttg
     4021 caaagaacaa cccttcaaag gtctcgcgct gttgctgaat ctggcccgcc aaccgaatgc
     4081 tgaatcccgt cggcaattgc aacttcgcaa actcttcttc caggtccgcg gcaatcgcgc
     4141 cgaggtctcg gttgacggga ttggcggtga tgtgaatcac ccgttggaaa tacttacgat
     4201 cgattttgac cggcccggcg ttcaatttca gagaggccac gttcttgagc agcaccggct
     4261 cgccgtttct ggccgtgagg atgatgtttt cgatgtcggt gagatccttg cggtgttctt
     4321 ccgccagcca ggcgctgatg tagtactcat tgccgttttg aggatcggta tagatgatgg
     4381 gatcggtctg gccgttgccg ttgagagaaa agagaacggc attcgccacg tccgtttcac
     4441 tgatgccgag aagggcggct ttctcgcggt caaccacgac attgacttcg ggatagttct
     4501 cttcgcggct gacttcgatg tccgcgagcc cttgaacctg gtgcatgatg ttctccacct
     4561 gccggatgac cgtgcgcgcg tgctcgaagt cgtagccgta gatctccaca tccaccgctt
     4621 tctgcgatcc gaagctggtg acgcgcttga cgagtccgcc gggatcaaaa aacatcgcga
     4681 cccctgggaa aagcttgatc gcttttggcc gaacctcgtt catgatctca acttgattcc
     4741 tggtgcgctt atccggtgag gccaggtaga cggagatgac cgacgtatga gggcccgtgt
     4801 tgggattgaa caacgaggag cgaccctggg ccaacacgcc ggtgctcgag acgatggttt
     4861 ccaattcgtg cggagggatg ttctcccgca acacccgttc gacttcggcg acttgttgct
     4921 cggttttctc gacccgttgg ccgaccggcg cgcgcaacac gatgcggaac tggctctcgt
     4981 cggagaccgg cagaaactcc gtgccgatca tcggtagcaa catcagcgaa gctgcgaaga
     5041 tgagcacgat gcccataatg aagagacgcc ggtgggcgag cacccagcgc aacgagtcct
     5101 catatccttt gtccagccac tcgtaccgct cgcggctcca gttcataagg cgcacgaacc
     5161 aagccggcat cgatcgatga gcctcccgct cgggcttgag gaacttgtaa cacagggcgg
     5221 gtgtgacggt gcgcgagacg aagaaagagg tgaacagagc gattgcgatc gtgagggtca
     5281 gcggaatcaa cagcaagcgc gcaatgccga cgacgaagaa aatcgggaga aaaacgacca
     5341 ccgtcgtgac ggttgatgcg aaaatcggca tcgccacttc gcgggctgct tcgaggatcg
     5401 cctcccatcg gcgcggcgtg gtgttcaaat ggcgctggat gttttccaat tccacgatcg
     5461 aatcgtccac cagtcgtccg atgccgagcg ctaggcctcc caaggtgaag acgttcagag
     5521 tttggcccgt gaagtagagc acgatgaagg tgaccatgat cgacagtggt atggccacgg
     5581 aaatgatgag tgtgctcgtc agattgcgca agaaaattag gatgacggca gccgccagca
     5641 gcgagccgtg cagcgcctgt tcgatgagat tcttaatcga ctgacggata tagaccgatt
     5701 ggtcgaacga gatgccgagc ttcacgcccg gagggatccc gaccatcttc ggcagcgcct
     5761 tccgcaaggc atccacgacc tccaccgtgt tggcgatcgg ctgtttgttc acccgtagat
     5821 aaaccgcccg tttgccgtcc gtccggacca cgttcgtttg aatgtccgag gaatcggtca
     5881 cggtgcccac atcccgcacg cgtacggggt tgccttgctg gttcactttg acaatcacgt
     5941 cttgaatcgg atcgaccgta cggaactgat tattggtgaa gacgttgtag tcgagattac
     6001 cggccttgat gtcgcccgag gggaggatca ggttcgactc cttcaccgct ttcacgacgt
     6061 cgagaatcga gagacctcgc gcgttcagca gggctgggtc gaggttgatg ttgatctgac
     6121 ggatcttgcc gccttcgacg gtggcggcgg cgacgttcgc gatttgctcg atctgcggcg
     6181 cgatcgtgtt gaacgcgagg tcgtacagtg cccgctcatc gagatcgtcg ctggaaacgg
     6241 tcacgaagga caccggaata ttggacacgt cgaatttgac gatgaacggc tgcagaatcc
     6301 ccggcggcag actgttgagg atctgcgtaa tgcgttgcat gacctccatc tgccccacgt
     6361 tgatgtccgc cccccagttg aaccagattt ggacgcctcc gatgccctgc ttggcaaagg
     6421 attccacatg ctcgacgttc gaggcggagc tgacggcttt ttcgatgggg tagacgacgc
     6481 tttgttcgat gtcgaggggc ggggcgcctt tataaatcac gccgacaaac gcaacaggca
     6541 cctgaatgtt gggaaacagg tcgaccggca atcgttgcag cgaggtggct ccgaggacta
     6601 ccatcgcgag cgacagcatc aagatgccga tgcgattcct gagcgccagg agcgtcagcc
     6661 acatacgtac gctatccctt ggcaggtgga gatgctggag gcgcggtgtt tgccggcgcc
     6721 ggcgcgggat ctgaaagtaa cggttgcgtt tgcacgggag tgccgtcgtg gacaaggtct
     6781 ttcccggaga cgatgacctg ttcagagccg gtcagccctt tggtgatctc gacgcggttc
     6841 tcatcgcgga ggccgatctc gacctcgacc cgctgggctt gtccgtcgcg aacaatatag
     6901 acgtactgca agtcttcgag ccggctgacc gcgtcgattg gaatttgaac cgcacgggca
     6961 tgttttccga ccaacacttc cacgcgggca aacatcccgc ccttgagcat gtgattcttg
     7021 ttgggaagat ccacttcgac ggtcatcgtc cgggtggcac gattcaacgc ctggacgatg
     7081 cgggtgaccg ttccctcgaa aacccggtcc ggataggcct cggcgcgaac ctcggccttc
     7141 tggcctatct ggacgagcgg gacatttttc tcgaccactt cgataagtat acggacggtc
     7201 tcgacgtcgt gcagactcag gatgccacgc gacatcgtag aagtgctggc ggttgcgccg
     7261 ctgacataag agccggggtc gaggttccgt tcggcgatat atccggcaaa gggcgcgcga
     7321 atgtaggagt aggcaaggtt cgtctctgcc tgggcaaggg ccacttccat ctgcttgacc
     7381 tgcgcccgca gtgaatcctg ggccgcgatc gcagcatcga atgacacctg ggcgttatcc
     7441 aagtcttgct gagagacaaa ctggtcctta ataagggcct gcatccggtc gagcgtgagt
     7501 ttcgcgttgc ggacattcgc ctcctgctgg gcgaccttcg ccctggccga tgccaaattc
     7561 gctttcgctt ggttgacggc atggacatag tccgtatggt caatctcgac caagagctga
     7621 ttagctttca caaagtcgcc tttatcgaca tagatcttgg cgatatagcc gtcgacgcgg
     7681 gaaaacaggt tcaccaattg gtttggcgtg atgtcggccg tataggccag tcggacgtcg
     7741 aggtcttgtt tcaggggcgt gacggtgccg acggtaatca cacgggcttt gcgggtatcg
     7801 gtcttcgctc cgctgctgag gcgaaataca accagggcgg tgaccgcgaa gaaaatgatc
     7861 acgccaagcg tcacgatggg atgttgcttc agggggttca tgcggcacca ccaagcgaag
     7921 gctgtttcaa accattcaaa aagaggtcga cataagtggc aaccgtctct tcgtgggatc
     7981 gatgcatcgg gacgccgaat atctcgtgca gcagccggtg atggacgacc atgccgatga
     8041 aggctcgggc ggcgaggagg ggatcgactt ttcgaaaggc ccgttcctgg atgcgcgtct
     8101 gaatgtaggt tgccaagtgt tcgtaaaaga ctttgtgctg tttcccaaaa aacatgtcgg
     8161 aaagctcatg gccttccaac gcgctgaaga gcaacagccg caggagcgtc ggatcgaccc
     8221 cgggtctgat ccggtaactg gcgattaggg tgaaaacccg ctggtcatcg cgtttcttgg
     8281 tggcttcctc cacggcttcc agcaactcgc tcacggtgac cttttcggca aggattgcgg
     8341 catacaaggc acgctttgtc gggaagtatt tgaagaccaa ggcctcgctg atccccgccg
     8401 cattcgcaat ctccttcgtg gtcgtcccgt taaagccctt tgctgcgaac agtgaagcgg
     8461 cggaggcaat caggctggcc tgacgctcct ggctagagac gcgcattgtt ttgcgaatgg
     8521 gacgcgatgg catagatata ttctgttaag tgagtgttta ctcactaaat ttatcatgac
     8581 ccaggaagag atgacaagaa gatggtcggt tcacgatgaa gtgacagata ttcctgtttc
     8641 tattcagttg gcgggttcga tgaacgggcg acgtagttct cagaggaagg caggtgagaa
     8701 ccagtcgagc gtcagagcgg actattggcc cgcaagaaag agtgcagccg gtttaattct
     8761 cgcacgttca ttttcagtaa tcgtacaccg aattgatggc ctgccgccca catcaccctg
     8821 gcctccatca caaagagcgg gtctcgtcca tcaggcagat aaaggaacaa cgtcagttcc
     8881 gttccggggg tgaccgcggt atcccctctg atacccagac ccttttgaga caggttcgtg
     8941 accatgccgt ctcccatgat caactcaccc ctcgcatcct gggctgagta catcaggccg
     9001 aaatcgaccg acgcgcggtc ggcttcccga cgattacagg gagcagtgaa ctgatgcgac
     9061 gacttgtgcg ttcgtactct caatgatttc atcgcgacac ctcctggttc ggaccttgat
     9121 ccccttcttc cttcggggac gctccattag ggactgtgat gaccccacaa gaaagaacgg
     9181 agttgatgct gtgcgtctac cggaacggtg aggagttgca cgccgaattt gcgtcccgca
     9241 acccacgaca cccgtccttc cgaaaggcag accgggtctt ctgctcccgg gaggtcgacg
     9301 aagagagcga gttccatccc gggtttgacc aggcgattac cgcagatacc gatcccgccc
     9361 tccgacaggt tcgtcaccat cccgtcgcca atgagcatgt gtccggaatc catacccgag
     9421 tacatcaaac cgaattgtgc ggggacgcgc atgatacgcc gacgatcgtc tggattgatt
     9481 agtgcgggcg cattgtcggc ggtttctaaa atccggtccc tcatgaacac ctcctgatgc
     9541 tgggttttcg cttacgtgat acgaaaggct gttcgattct gattggatgt cgaaagcgtc
     9601 acatttctcc aaatgacctc ttgcacacgc ctccgttcct cagcagtcag ccgagggaat
     9661 cgaatggcga aggaaaatcc ctttacccag gttacttgtc cccccgtaag gcacaaggga
     9721 g
//
