LOCUS       DATDNZ010000001         5920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2620924, whole genome shotgun sequence.
ACCESSION   DATDNZ010000001 DATDNZ010000000
VERSION     DATDNZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620924"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..302)
                     /locus_tag="VI432_00005"
     CDS             complement(57..302)
                     /locus_tag="VI432_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496521.1"
                     /translation="MTAKTKIFKHTQADGLSRLIDKWLLEDKPKIVAMSQSTRELSIG
                     AEIIVVIVYVSNRKRSKKRVAGSSRSKSDKIRPQRKK"
     gene            complement(419..652)
                     /locus_tag="VI432_00010"
     CDS             complement(419..652)
                     /locus_tag="VI432_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496522.1"
                     /translation="MPHKPELFLPKGGMEGAEVRFALEKMGNDGVRIQHTDERIPKLV
                     FPHGHGGERELIGAEAVVAGLREVHRLAEERRR"
     gene            complement(733..1581)
                     /locus_tag="VI432_00015"
     CDS             complement(733..1581)
                     /locus_tag="VI432_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4496523.1"
                     /translation="MKKIGLALGGGGAKGLAHIGIIKALKEANLPIDYIAGTSMGAVV
                     GAWYASKLETEELEEISVNTKRRNLFPIAEILSANKKSLFVGDAIEKLLKKYFDKDKI
                     EDCKIPFAAVATNMKDGEPMPIKSGYLADAVRASSSIPVVFSPVHLEGDVLVDGGLSN
                     PVPADIVKEMGADYVIAVDVSSKWPDHNHDLFESRGFYATMMESMAIIEYQLAKNILK
                     EADLVIRPSVMRFNWPDFDNAEEIISLGYREGRRFIKEIALKTGYPIPEQSPLERFFD
                     FFSSGE"
     gene            complement(1733..4321)
                     /gene="uvrA"
                     /locus_tag="VI432_00020"
     CDS             complement(1733..4321)
                     /gene="uvrA"
                     /locus_tag="VI432_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4496524.1"
                     /translation="MNDKIKIKGAREHNLKNVNLEIPRNKMVVFTGLSGSGKSSLAYD
                     TIFAEGQRRYIESLSAYARQFLGRLDKPDVDEIEGLSPAISIDQKAHSANPRSTVATI
                     TEIYDYLRVLYARAGKPYCPDCGHVIRKLTMSEIVDSIIALYEEDKKNQNLVVLAPAV
                     RGRKGEYYQLLYDFLNAGFSEARIDGKLHSLRNRINLDRYKSHTIEIIVDRIPGGLDF
                     SDKDNRLRLSEAVESALKYGNDVVDVILGDKTNSLSAKFTCPRDGFSFPEIEPRLFSF
                     NSPYGACPTCHGLGVEDVWSDNLCNDCDGKRLKREALSILIDDKNIVTVTQNSISDAY
                     NFFDTLRNKLSKQELEVAEVPLREIKNRLKFMIDVGLEYLTLERRAGTLSGGESQRIR
                     LASQIGSRLVGALYVLDEPTIGLHQRDNDKLIKTLHDLRDLGNSIIIVEHDEETIRES
                     DYLVDFGPGAGVHGGKIIAQGEIPSIIKPKIGEKKQDSLTLRYLRGEEFIPVPKKRRK
                     IDPVKPDRSGNGAGNKTNFLKIRGATENNLKNLNIDIPLGRFTAVTGVSGSGKSTLVY
                     DILYKFLANKFNKANYKVGAHKSISGTEYLNRIINIDQSPIGRTPRSNPATYIGAWTH
                     IRDLFASTEGARARGWKPGRFSFNVKGGRCENCEGHGYIGIEMHFLPTVYVTCDTCHG
                     KRFDRETLEVTYAPSGKKSDKKNIYEVLKMTIEEAREFFKDIPWLNDKLTIIDEVGLG
                     YLELGQSATTLSGGEAQRVKLASELGKRDTRQTIYLLDEPTTGLHFADVHKLLDVLNR
                     LVERGNTVVVIEHNLDVIKTADWVIDLGPDGGEKGGEVVAVGTPEHISKSAGSATGEY
                     LRKSLK"
     gene            complement(4321..>5920)
                     /locus_tag="VI432_00025"
     CDS             complement(4321..>5920)
                     /locus_tag="VI432_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY4496525.1"
                     /translation="EIGSVINRKELVEKLIRMQFKRTNADLKRGTFRLRGDNWEIMPP
                     NEEIIYNFALDPSTESKSRTQARSGQAKIKEIYAIDPVEGFNKNTPKIHEVFISPARH
                     FITLSDERELAIKNIEEELKERLKLFEREGKLLEAERLERRTKSDLAMIREIGYCNGI
                     ENYSRHLSGRAVGEPPDTLLDYFCTRHSFSDGGPHQCDFLMVIDESHVTVPQLNGMHA
                     GDASRKKTLIEYGFRLPSASDNRPLRFEEFEKRMPQVIYTSATPGEYELEKSGKEGII
                     EQIIRPTGLIDPKVTMIPAKGQVEDLIPRIKERIDKGDRVLVTTLTKKMAEDLSNYLE
                     ELKFKVNYLHSDVKTLDRIRVLTGLRRGTYDVIVGVNLLREGLDLPEVSLIAILDADK
                     EGFLRSETSLIQTIGRAARNVNGEVLIYADNITGSIKRAVDETERRRKLQIAYNKKHN
                     ITPKTIIKEIKDILPAEEILELETKPLPKSDNAIRLLIKEKEKEMKKAAEALDFELAS
                     VLRDEIYVLGAQLKEGKSKTKKSK"
BASE COUNT         1643 a         1412 c          951 g         1914 t
ORIGIN      
        1 tagcacatca tgatagtttt gtcaaaaaag aagcccgtaa ttcgggcctt tattgtttat
       61 ttcttacgct gaggtctaat tttatcagac ttagacctag aactcccagc aactctcttt
      121 ttgctccttt ttctgtttga aacataaaca atcacgacaa taatctccgc gccgattgac
      181 aattccctag tggactgact catagcgaca attttgggct tgtcttccaa aagccactta
      241 tctattaaac ggctaagccc atctgcttgc gtatgcttga aaatctttgt tttagcggtc
      301 aagttgcgcc tctttttgaa tgtacaagtt caaaccgaac tccataaata tcaaacaact
      361 tttaacaatg atttgtcaaa tagaaaaaga ccctattatg gggtcttgct atcgcggact
      421 aacgccgcct ctcttctgcc aaccggtgaa cctctctcaa tccggcaact acagcttcag
      481 cgccgatgag ctctctctcc cctccatgtc catgagggaa tacaagttta ggaatgcgtt
      541 catcggtgtg ctgaatacgc actccgtcat ttcccatctt ttcaagagcg aagcgaactt
      601 ctgctccctc cattcctcct ttgggtaaaa acagctcggg tttgtgtggc attttcttcc
      661 tccttttttg ttatagaact attgagactg aagctttaat tctacattaa tatataaagt
      721 gcgcaaacac ggttattcac ctgatgaaaa gaaatcaaaa aacctttcaa gcgggctttg
      781 ctctggaatc ggatatccgg tttttaacgc aatctcttta ataaaccttc gtccctcgcg
      841 atagcctaaa cttataatct cttcggcatt atcgaagtct ggccaattaa aacgcatgac
      901 agaaggacgg attactaaat ctgcttcttt caaaatattc ttagctaact gatattcaat
      961 tatggccatg gattccatca tggtcgcata aaaacctcta ctctcaaaaa ggtcatgatt
     1021 atgatcgggc cacttacttg atacatcaac cgcgataacg taatcagctc ccatttcttt
     1081 aacaatatcg gccggaaccg gatttgataa tccgccatca accagaacgt cgccctctaa
     1141 atgaacagga ctaaaaacaa caggaataga agatgatgct ctaaccgcgt ctgctaaata
     1201 tccactcttg attggcatcg gttcgccatc tttcatattt gtcgcaacag ccgcgaaagg
     1261 aattttacaa tcttcaattt tatctttatc aaaatatttt tttaaaagct tttctatcgc
     1321 atctccaaca aataaacttt tcttattggc ggacaatatt tccgcaatag gaaataaatt
     1381 tctgcgttta gtattaacgc ttatttcttc taattcttct gtttcgagtt tagacgcata
     1441 ccatgcgcca acgacagcac ccatgctcgt tcctgcgata taatcaatag gtaaatttgc
     1501 ctctttcaag gccttaatga ttccaatgtg agcaagtccc ttagctcctc cccctcctaa
     1561 agcaagaccg atttttttca tatcgttaaa aaattagatc ctcctgactt atccattaca
     1621 tttttaatat ccatgatttt acaggtttta ttttcattta acgattgttc aaattttcta
     1681 atgggattta tcaattaata ttgtacattc aaatatctat gaatccaaaa aattatttta
     1741 gactcttacg cagatactct ccagtcgcgc tccctgcgga tttagagata tgttcaggcg
     1801 tgccaaccgc aacgacttct cctccttttt ctcctccatc aggcccaaga tcaattaccc
     1861 aatctgcggt ttttataaca tctaaattat gctcaatcac gacaaccgtg ttgcccctct
     1921 caaccaatcg attcaaaaca tccaaaagtt tgtggacatc agcgaagtga aggccagttg
     1981 ttggctcatc aagcaaatat atcgtctgcc tcgtgtctct tttccctaat tctgaagcta
     2041 atttgaccct ctgagcctct ccgccggata aagttgtagc cgactgcccc aactccaaat
     2101 accccaaacc cacctcatca ataatcgtta atttatcatt caaccaagga atatctttga
     2161 aaaactcacg agcttcctca atggtcattt ttaaaacttc gtatatattt ttcttatcgc
     2221 ttttctttcc gcttggcgca taagtcactt caagggtttc tctgtcaaag cgcttgccat
     2281 gacaagtatc acaagtaaca taaactgtcg gcaaaaaatg catttctata ccaatatagc
     2341 catgcccctc gcaattttca cacctccctc cttttacgtt gaagctaaat ctacccggct
     2401 tccatcctcg agctcgcgcc ccctcggttg aagcaaatag atctctgatg tgagtccatg
     2461 cgccgatgta ggtcgccgga tttgacctag gagttcttcc tatcggagat tgatcgatat
     2521 ttataatcct attaagatac tccgttccgc tgattgattt atgcgcgcca actttataat
     2581 ttgctttatt aaatttattc gccaaaaatt tgtataaaat atcatacaca agagtgcttt
     2641 tgccggaacc agaaacgcca gtcaccgctg taaatctacc aagaggaata tcaatattta
     2701 aatttttgag attgttttct gtcgctccgc gaattttaag aaaattagtt ttattacccg
     2761 ccccgttacc agatctgtct ggttttactg ggtcaatctt acgcctcttc tttggaacag
     2821 gaataaattc ttcgcctcgc agataacgca gagttaagct atcttgtttc ttctctccga
     2881 ttttaggttt tattatcgat ggtatctctc cttgagcaat aatctttccg ccatgcacac
     2941 ccgccccagg accgaaatca accaaataat cagattctct gatcgtttct tcatcatgtt
     3001 caacgatgat aatactattt ccaaggtcac gaagatcatg caaagtctta atcaatttat
     3061 cattatctct ttgatgcagt ccgatagtcg gctcgtctaa cacataaaga gcgccaacaa
     3121 gtcgcgatcc gatttgagac gccaaccgta ttctctgcga ctctccgcca gacagcgttc
     3181 ccgcacgcct ctcgagcgtc agatattcca aaccgacatc aatcataaat ttaagccggt
     3241 ttttaatttc acgaagagga acttctgcta cttccagttc ctgttttgat aatttattcc
     3301 tcaatgtatc gaaaaaatta taagcgtcgg agatagaatt ttgtgtaacg gttacgatat
     3361 ttttatcgtc tattagaata ctcaacgcct ccctctttaa tcttttgcca tcgcaatcat
     3421 tgcataaatt atctgaccaa acatcttcca cccctaagcc atgacaggtc gggcacgctc
     3481 catagggact attaaacgag aataatcttg gctctatttc aggaaatgaa aatccgtcgc
     3541 gaggacaagt aaatttagct gataatgaat tagttttatc gcccaatatg acatcaacca
     3601 catcattacc atatttcaag gctgattcaa ccgcttccga caaacgcaac ctattatctt
     3661 tatcagaaaa atctagccct ccaggaattc gatcaacaat aatctcaatc gtgtgcgatt
     3721 tatacctatc caaattaatt ctgttcctta aagaatggag cttgccgtca atacgcgcct
     3781 cagaaaaacc agcgtttaga aaatcgtaaa gcaattggta atactctcct tttcttcctc
     3841 gaaccgccgg tgcgagcaca accagattct gattcttttt atcctcctca tacaaagcaa
     3901 taatcgaatc aactatctct gacatggtta attttctaat cacatgtccg caatcaggac
     3961 aatatggttt tccggcgcgg gcgtataaaa cacgtaggta atcgtaaatt tcagttattg
     4021 tggcaactgt cgagcgagga ttagcagaat gggctttttg atcaattgat atcgcgggag
     4081 atagcccctc gatctcgtcc acatccggct tatcaagacg gcctaaaaat tgtctagcat
     4141 aagctgataa ggattctata tatctcctct gaccctctgc aaagattgtg tcataagcaa
     4201 gcgatgactt tcccgaacca gaaagcccgg taaaaacaac cattttgttg cgcggaattt
     4261 ctaaattaac atttttaaga ttatgctctc tagctccctt tatcttaatt ttatcgttca
     4321 ttattttgat tttttagttt tcgatttacc ctcttttaat tgcgcgccca aaacatatat
     4381 ttcatctcgc aaaactgatg ctaattcgaa atctaatgcc tctgcggctt ttttcatttc
     4441 tttctctttt tctttaatca gcaatcttat agcgttatca gatttaggca atggttttgt
     4501 ttcaagctcc aaaatctcct ctgcgggtaa tatatctttt atttctttaa taattgtctt
     4561 gggtgtaatg ttatgttttt tgttatatgc aatttgaagc ttccttctcc tctctgtctc
     4621 atccaccgcg cgtttgatgg atccggtgat attatctgca taaattaaaa cctcaccatt
     4681 aacatttctt gcggcccttc ctattgtttg aataagagat gtttcgctcc taagaaaacc
     4741 ttctttgtcg gcatctaaaa tagctataag cgatacttcc ggcaagtcta atccttcacg
     4801 taaaagattc acgccaacaa tcacatcata tgtaccccta cgaaggccag taagcacgcg
     4861 gattctgtcc agagttttca catcgctatg aagataattt actttaaatt taagctcctc
     4921 gagataattc gataaatctt ctgccatttt tttagtaaga gtcgttacaa gaaccctatc
     4981 tcctttatca attctttctt taatacgagg aattaaatct tcaacctgac cttttgctgg
     5041 aatcatagta acttttggat caataagacc ggtgggtctg atgatctgct caataattcc
     5101 ttcttttccg cttttttcta attcatattc acctggggtg gctgaagtgt atataacctg
     5161 aggcatacgc ttttcaaatt cctcgaatct taacggacgg ttgtcagatg cagacggaag
     5221 cctaaaaccg tattcaataa gcgtcttttt acgcgacgca tctcccgcat gcattccatt
     5281 caattgtgga accgttacat gcgattcatc aatcaccatt aggaagtcgc attggtgggg
     5341 cccgccgtcg ctaaagctat ggcgggtaca aaaataatct aaaagagtat ctggcggctc
     5401 tccgacagcc ctaccggata aatgacggga ataattctct atgccattgc aatatccaat
     5461 ttcgcggatc atcgctaaat ccgattttgt tctcctctct aacctttctg cttcaagcaa
     5521 ctttccttcc ctctcaaaca actttaatct ttccttcaat tcttcttcta tatttttaat
     5581 cgctaattcc ctttcatcac ttagagttat aaagtgacga gcaggagaga tgaaaacttc
     5641 atgaatttta ggagtatttt tgttaaatcc ctcaacgggg tctatggcat atatttcttt
     5701 aatcttagct tgccctgatc tcgcctgcgt tcggcttttc gattctgtcg aagggtctaa
     5761 agcaaaatta taaataattt cttcatttgg aggcataatc tcccaattat caccacgcag
     5821 acgaaacgtt cctcgtttta aatcagcatt agtcctctta aactgcatcc tgattagctt
     5881 ttcaaccaat tcttttctat tgatcacgct tcctatttca
//
LOCUS       DATDNZ010000002         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_791157, whole genome shotgun sequence.
ACCESSION   DATDNZ010000002 DATDNZ010000000
VERSION     DATDNZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791157"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="lysS"
                     /locus_tag="VI432_00030"
     CDS             <1..1131
                     /gene="lysS"
                     /locus_tag="VI432_00030"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY4496526.1"
                     /translation="RKEPTIEVKKWQLLSKSLRPLPEKWHGLTDVEERFRKRYLDLLM
                     NPVTRGVAEKRIKILKTAREFFERSGYLEVDTPILQPIYGGGLARPFKTHHNALNIPL
                     YLRISDEMYLKRLIVGGFTKIFEICKDFRNEGMDHSHNPEFTLLEAMTGYQDYKFTMN
                     LVEDFYEYVVKKVNKDLKVKYGNQVINFKRPWKKSSMKDIVRAKTGFDFDSTPNMIDA
                     RHKAMRLGISQEKIEQQGCIGEIMNLIFEEKVQPQLIQPTIVYDYPVETSPLAKKSNK
                     DPRFVERFEHFILGHEHGNHYSELNDPIDLQERFVEETKKERAGFEEAHQNDKDFLEA
                     IEYGMPPVTGLGISIDRLVILLTGVESIKEVILFPTLRPKNE"
     gene            1136..1978
                     /locus_tag="VI432_00035"
     CDS             1136..1978
                     /locus_tag="VI432_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY4496527.1"
                     /translation="MLRFVSKLKKKLGYIKRGLFGFVIDIIERTPKISADIEKTIVGK
                     SVENRPIEYYQIGQGTTKLMFMAGIHGNEVGTVKLVKHLINFLVVENQGLKNRFTFFV
                     IPRLNPDGDALARKNPDYFRGGRIGRFNARRVDLNRNFETPSFKKESIYSFGKRYQEK
                     EKVYCGEYGNSEPETKAFTDLIFKENIKIIFAFHNAGRDVMGNNLDLSQKLTRVYADK
                     SRFRFVPNQEWVELQQSGTLKEWSEIHNIAYVEIEGSTRWGSDWKTQKEAIRATIEIN
                     ESIL"
     gene            complement(1968..2414)
                     /locus_tag="VI432_00040"
     CDS             complement(1968..2414)
                     /locus_tag="VI432_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4496528.1"
                     /translation="MSTKKKIVIFDFNRTLYNPEHRRLMPRIELTLKTLIRRGYKLYL
                     ISTANPSRNKLIRELGLKKYFRRAIITKNKKAAIKDIVDHLRINSKNSFVIGDRVRSE
                     ISYGNIAKMKTIWLRAGKFSAEKPRLKREIPTYTVTKLSATLRFIK"
     gene            complement(2510..3583)
                     /locus_tag="VI432_00045"
     CDS             complement(2510..3583)
                     /locus_tag="VI432_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496529.1"
                     /translation="MRANRVGLSDPKYRFLFGPRGFLIDRRDLDELTRLAKISTTFLK
                     NADEFYQHEMARGNSHLASILQHGIPRKYVPLTLKEGLPQTFMIDTMWTANGWRIVEI
                     DATNRNGLGYPLVMRHLYNLPNVWKGNDRQWQEAGFGETIQVMTHHCRFYEPYYRYFL
                     EAIKGNLITETEINNRLPEIQAASSLLDMPIFHQGSFVESLLQVAKVALIGIPPKHHL
                     SSKAILSLPWEVKEFFADGVCEFLPETRLLRNSTPFPVDEFFVKFLQSGGAHGTFYND
                     QIQFERLRNKRPRAIWQNALPITKRLIEFLDEDNILKVSESFIRLSIYVTPQGEIVDA
                     DVTASNEIIVHGGNRSIMTVPTL"
     gene            complement(3810..>4010)
                     /locus_tag="VI432_00050"
     CDS             complement(3810..>4010)
                     /locus_tag="VI432_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496530.1"
                     /translation="YSPMNMVEVGFKTLIFKGKLGYTHYPQDYPQFTTLFTVYPTYTF
                     IANILYISLWKSKPPGKIVCLT"
BASE COUNT         1231 a          731 c          884 g         1164 t
ORIGIN      
        1 cgcaaagagc cgacgatcga agtcaaaaaa tggcagctgt tgtccaaatc cctccgtccg
       61 cttcccgaaa aatggcacgg cctcacggat gtcgaagagc gcttccgcaa gcgttatctg
      121 gatttactga tgaatcctgt aacgcgcgga gttgctgaaa aaagaataaa aatattaaaa
      181 acggcaagag aattttttga gcgtagtggt tatttggaag ttgatactcc tatccttcaa
      241 cctatttatg ggggaggatt agcgcggccc ttcaaaacac atcataatgc actgaatatc
      301 cctctttatc taagaatctc tgacgagatg tatcttaaga gacttattgt gggaggattc
      361 accaaaattt ttgaaatttg caaggatttc agaaacgaag gaatggacca tagccacaat
      421 cctgagttta ctcttttaga ggccatgaca gggtatcagg attataaatt tacgatgaat
      481 cttgtcgagg acttttatga atatgtggta aaaaaagtca ataaggattt gaaagtgaaa
      541 tacggtaatc aagttataaa ttttaaaaga ccttggaaaa aatcttccat gaaggatatt
      601 gtaagagcca aaacaggatt cgattttgat tcaactccca atatgatcga tgcgcgacat
      661 aaagcgatgc gcctcggaat ttcccaagaa aaaattgagc agcaaggctg tatcggcgag
      721 ataatgaatc tcatttttga agaaaaagtg caaccacaac tgatccaacc aactattgtg
      781 tatgactatc cagttgaaac ttcgccgctt gctaagaaaa gtaataaaga tccacgtttt
      841 gtagaacggt ttgaacattt cattttgggg catgagcatg gtaatcatta cagtgaatta
      901 aatgatccaa ttgatttaca ggagcgtttt gtggaagaaa caaaaaaaga acgcgctggc
      961 ttcgaggagg cgcatcaaaa cgataaagat tttctggaag caatagaata tggcatgccg
     1021 cctgttactg gcctcggtat tagtattgat cgtttagtaa tacttcttac gggagtggaa
     1081 agtattaagg aagtgatttt gtttccaaca cttaggccaa aaaacgaata gcgaaatgtt
     1141 gcgatttgta agcaaattaa agaaaaaact cggctatatt aagcggggtc tttttgggtt
     1201 tgtgatagat attattgagc gaacgccaaa aatttccgcc gatattgaaa aaacaattgt
     1261 aggtaaatca gtggaaaata ggcctatcga atattatcaa attggacagg ggacgacaaa
     1321 attgatgttt atggcaggga ttcacggaaa cgaagttggt acggtgaagt tggtaaagca
     1381 tcttataaat tttttggttg tagagaatca aggccttaaa aatagattca cattttttgt
     1441 cattccccgt ttgaatcccg atggagatgc attggcgcga aaaaatcctg attattttcg
     1501 aggtggaagg attggaagat tcaatgcgcg ccgagttgat ctgaatagga attttgagac
     1561 gccaagtttc aaaaaggaat ctatttattc gtttggcaag cgctatcagg agaaagagaa
     1621 agtatattgc ggtgaatatg gaaattctga acctgaaacc aaggctttta cggatttaat
     1681 atttaaagaa aatataaaaa ttatatttgc gtttcacaat gccggcagag atgtaatggg
     1741 aaataatctt gatttatctc aaaagttaac ccgcgtgtat gcagataaaa gcagattcag
     1801 atttgttccg aaccaagaat gggtggagct acagcaatcc ggaacgctca aagaatggtc
     1861 ggaaatacac aacatagcgt atgtggaaat cgaaggatct acccgctggg gaagcgattg
     1921 gaaaacacaa aaagaggcaa taagagcaac aatagaaata aacgaatcta ttttataaat
     1981 ctaagagttg ccgagagttt tgtaacggta taagttggga tttctctttt gaggcgtggt
     2041 ttttctgcag agaattttcc tgctcgtagc cagattgttt tcatttttgc aatattgcca
     2101 tagcttattt cgcttcgcac gcgatctcct atcacgaaac tatttttaga attgattcga
     2161 agatgatcaa caatatcttt aattgccgct tttttatttt tagttataat tgcgcgccga
     2221 aagtattttt ttagaccaag ttcgcggatg agtttattgc gagatggatt agctgtactt
     2281 ataagatata atttatatcc acgccgtatt aaggttttta gtgttaattc tatcctcggc
     2341 attaagcggc gatgttccgg attatagagc gtcctgttga aatcaaaaat aacaatcttt
     2401 ttcttggtgg acatagtttc agattaccga gagagtttat tgacgtcaat aaggaataaa
     2461 accccagaga gatcatgtga tctctctggg gttttatgtt tctgcacacc tataaggtcg
     2521 ggactgtcat gatggatcta tttccgccgt gaacgatgat ttcattcgag gcagttacat
     2581 ctgcgtccac aatctcgcct tgcggagtta catatatcga gaggcggata aaactctccg
     2641 atactttgag aatattatcc tcatctaaga attcgatgag gcgcttagtg atcggcaaag
     2701 cattctgcca aatggcgcgc ggccgcttgt ttcttaaccg ctcaaattgt atctgatcat
     2761 tgtagaacgt accatgcgcc ccaccacttt gcagaaactt tacaaagaac tcgtcgaccg
     2821 ggaaaggtgt tgagtttcgg agaagtctag tttctgggag aaattcgcaa actccatccg
     2881 cgaagaattc ctttacttcc caaggcaggg aaagaatcgc ttttgaagaa agatgatgtt
     2941 ttggcggaat gcctattaga gccacttttg ccacctgaag caatgattca acgaagcttc
     3001 cttgatgaaa aattggcata tcgagaaggg atgatgcggc ctgtatttcc ggtaagcggt
     3061 tgtttatttc agtttcggtg ataagattcc ccttgatcgc ttccaagaaa tagcgataat
     3121 acggctcgta gaagcggcag tgatgagtca tgacctgtat tgtctcgccg aagccggctt
     3181 cctgccactg tctgtcattt cctttccaaa cattaggaag attatacaga tgacgcatta
     3241 caagcggata tcccaatcca ttacgatttg tagcgtctat ttcaactatg cgccatccat
     3301 tcgcggtcca cattgtgtca atcatgaatg tttgagggag tccttccttt aatgtcagag
     3361 gtacatactt tctgggaatc ccgtgttgga gaatggatgc taagtgcgag ttacctcgag
     3421 ccatctcgtg ttgatagaac tcgtccgcat ttttcaggaa agtcgttgag attttggcca
     3481 gacgggtaag ttcatcgaga tctcgccggt caattaaaaa acctcgcgga ccaaaaagaa
     3541 acctatactt tggatcgcta aggccgaccc tattagcacg cataagcagt tcctgataca
     3601 tcaaacacct ccttggttga attgttaatg agccaacaaa aaaaccagcc ctggaaggct
     3661 ggcgtcgtga ctcttaatta caatatgttt ttatctacga agacgcggcg tcaaccttcc
     3721 agcttttggc tggcttttct ttgcttcatg ctttttagca aaaaggttaa cgcagtaata
     3781 catctctcta atctactgta agcagaattt tatgtcaaac aaacaatctt gccgggcggt
     3841 ttgcttttcc acagggaaat atatagaatg tttgctatga atgtataagt gggataaact
     3901 gtgaagagag tggtgaattg tggataatct tgtggataat gtgtataacc caatttacct
     3961 ttaaaaatta aggttttaaa gcccacttca accatgttca taggcgaata
//
LOCUS       DATDNZ010000003         8629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2885214, whole genome shotgun sequence.
ACCESSION   DATDNZ010000003 DATDNZ010000000
VERSION     DATDNZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885214"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..279)
                     /locus_tag="VI432_00055"
     CDS             complement(121..279)
                     /locus_tag="VI432_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496531.1"
                     /translation="MLTKICIWKHKEWETFPGTWGIYAHSDEEIISLIKSNPEKYGIF
                     EDWYKAEK"
     gene            complement(407..622)
                     /locus_tag="VI432_00060"
     CDS             complement(407..622)
                     /locus_tag="VI432_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496532.1"
                     /translation="MAHKLQKERRIEKMISQEWREEMQRFGQSCYDEGYRQGLLGKEY
                     TGRTFIGYYGWLDGSFHAFRKEEGRKM"
     gene            complement(694..873)
                     /locus_tag="VI432_00065"
     CDS             complement(694..873)
                     /locus_tag="VI432_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496533.1"
                     /translation="MRKVSDKQLMFILHLKFTGCFLQELEIFESGNMEIKFVDKGGNF
                     HHQTLNENGTLTTHD"
     gene            complement(992..1669)
                     /locus_tag="VI432_00070"
     CDS             complement(992..1669)
                     /locus_tag="VI432_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4496534.1"
                     /translation="MMKIYIISDCHGNIDGLFRALEKKKLVDSSGNRIVGTDHFIVSI
                     GDLANCVDDSVSGDIECLNLVGSIIDSMICGNHEIPYLDISNSFAGFSWNLDINDKIQ
                     TLMSEDFIGAAFLVGNTLISHAGLSVQFSNLEQTPEKIFNTIEDHWKAKNYNHSWFSS
                     VGYARGGRHPIGGLFWCDFDREFMPTLFPQIVGHTPKYVRMKDNALCIDVGAKDQDTE
                     PFILELA"
     gene            complement(2213..2428)
                     /locus_tag="VI432_00075"
     CDS             complement(2213..2428)
                     /locus_tag="VI432_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496535.1"
                     /translation="MKLLQKGRPKKMIDDFSKYFTIEIYGEKGWEVVWSCFDWDDAIS
                     HVNRYSLEYQVRITSPIKEKERKKEIK"
     gene            complement(2487..2693)
                     /locus_tag="VI432_00080"
     CDS             complement(2487..2693)
                     /locus_tag="VI432_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496536.1"
                     /translation="MAYTERNFRSKKELMTALSEGEKIRVFQPGLGIVPENGTVYLEG
                     PHYPEPHKWYAEGIMAEGLLVKVK"
     gene            complement(3194..3568)
                     /locus_tag="VI432_00085"
     CDS             complement(3194..3568)
                     /locus_tag="VI432_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496537.1"
                     /translation="MIQNAPPRVKAARGRQKGSSDVEKAWRAGFALLSDENPIAFSAK
                     LTKASEYPMLRTSVNRSNGEDTGKVGPYRAALEHFGLCETILAEDEKTIVVAIARPLE
                     NDWNEIVNEIVNEIDENDDENV"
     gene            4531..4800
                     /locus_tag="VI432_00090"
     CDS             4531..4800
                     /locus_tag="VI432_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496538.1"
                     /translation="MTELEEKIYKDLENQYFSDEKSKICNVCPQCKAIKMFASCNHGD
                     DWPRYTDTEKIEVFPGDIVWSEDEYNYNSFTPYYEKGKGRVYSYQ"
     gene            4825..4995
                     /locus_tag="VI432_00095"
     CDS             4825..4995
                     /locus_tag="VI432_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496539.1"
                     /translation="MTKRESTYTVEWIRSILPDEINTCSGLTARVAFARLIQALNNSG
                     PIIVKKIKIERK"
     gene            complement(5189..5767)
                     /locus_tag="VI432_00100"
     CDS             complement(5189..5767)
                     /locus_tag="VI432_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496540.1"
                     /translation="MKRFLLTILAFLILAPTAGSYYKHDKLNEVASWIAMRPVEVFCY
                     LDDEDEAPWTYGAWGYVDKPLGKQSYEALDFRLCEGAQNINDTNILKWQRALGVLALT
                     HESYHLRRWGGAGDEAKVECKAIRHWRYTAQKFGATNEIIEDLWPLALALHYKLTNYV
                     DYDKNFNQIRPYYDPFCKVPPVFTQTDDPSDT"
     gene            5828..6052
                     /locus_tag="VI432_00105"
     CDS             5828..6052
                     /locus_tag="VI432_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496541.1"
                     /translation="MKNPIWTLEEIEKLSMFGGNVGWFYGLVKHHETGRVWVYEIFPG
                     TGYARTGWIYHPKPLWWVIKDIWRATKRVS"
     gene            6116..6340
                     /locus_tag="VI432_00110"
     CDS             6116..6340
                     /locus_tag="VI432_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496542.1"
                     /translation="MEQITNLEMAVNIVKIGETPEERTKIVNKLLEGEVDTETDNGKG
                     LLHRRALLITALSEQKIWPMKENKNAKSNK"
     gene            6654..6902
                     /locus_tag="VI432_00115"
     CDS             6654..6902
                     /locus_tag="VI432_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496543.1"
                     /translation="MIRTCNKCGAKVDNKKPDPCFGKYLPEIAFCCCGHGNNTYAYCV
                     GWENCLPNEGPTDWREENDPTYWCLRGIEAIEYMENLK"
     gene            6899..7090
                     /locus_tag="VI432_00120"
     CDS             6899..7090
                     /locus_tag="VI432_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496544.1"
                     /translation="MKLTKEEWLDLNKRFTKINREYFEVTSCCAICGKSFFDITGMSG
                     QVSSMEYPGLYNHFPNCPK"
     gene            7087..7287
                     /locus_tag="VI432_00125"
     CDS             7087..7287
                     /locus_tag="VI432_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496545.1"
                     /translation="MKYTFEAYKEDGSPRKSAGMFRPPYEEVFKTIKDSGQLYRYMQG
                     YEINIPYSSGQFDKPLYEDEDE"
     gene            7274..7480
                     /locus_tag="VI432_00130"
     CDS             7274..7480
                     /locus_tag="VI432_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496546.1"
                     /translation="MKMNRPNIKEIEKDLKSHITSMRSSIWRDYADSVEENINILINY
                     IEELENRLFIINSRTSHEYWSHKS"
     gene            7480..8040
                     /locus_tag="VI432_00135"
     CDS             7480..8040
                     /locus_tag="VI432_00135"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY4496547.1"
                     /translation="MSGHLEVICGPMFSGKTEEMLRRLRRVEIAGQKSIVFKPVIDNR
                     YSVTEIASHVGNKREAYAVKDIYALLDIIAQGTYDVIAIDEVQFFEFEIAVTIQGFLE
                     ANKRVIVSGLDLDFRKEPFGIVPELLAMAKFVVKLRAVCQVCGEDAMYTQRLLNGKPA
                     PLSGKTVIVGGQEQYEARCEKCWQEG"
BASE COUNT         2611 a         1717 c         1747 g         2554 t
ORIGIN      
        1 atccaagcat ttgaacattt catctgccca atattctgta aggcagattt tttcgggcga
       61 ataattataa agaataatct tttgaaattc ctcgtaagta tttccttttc gttccatttt
      121 ttatttctcc gccttgtacc aatcctcaaa aattccatat ttctcaggat tggatttaat
      181 tagagaaata atttcttcgt ctgaatgagc ataaattccc caagttccgg gaaaagtttc
      241 ccattcttta tgcttccaaa tacaaatttt ggtaagcatt attatttctc ctttccgctt
      301 ctatgtattg catagaaaca tgcggcaaaa attagccata ggaaaaacca ggacaaaata
      361 ccttggaaca actcccaatt catttttatc ccctttcact tcaggattac atttttctac
      421 cctcctcttt cctaaatgca tggaagctgc cgtccaacca gccgtagtat ccaatgaagg
      481 ttctaccagt gtattcttta ccgagtagtc cctggcgata accttcatca tagcacgact
      541 gtccaaatct ctgcatctct tcacgccatt cctggctaat cattttctct atcctccttt
      601 ctttctgtag tttatgcgcc aaatgggatt cgaagcagca tttctagtct caaccctgca
      661 tttccaaagc agaagaaaaa tgcaggattc aaactagtca tgagtagtta gtgtgccatt
      721 ctcattgaga gtttgatgat ggaaatttcc gcctttatca acgaatttta tttccatatt
      781 tccactctcg aagatttcaa gttcctgcaa aaaacagccg gtgaacttca aatgaagaat
      841 gaacatcaac tgcttatctg acacttttct cacctccttt ccgctaaaaa aagagactag
      901 gaataatctc gtttctattc tcttatgccg gatctttcca tatccgatct agcctcaaga
      961 ctccatttta cattaaaata aaatggagtt ttcaagctag ttcaagaata aagggctctg
     1021 tatcttgatc ttttgcacca acatcaatac acagagcgtt atccttcatt cttacatact
     1081 ttggagtatg ccccacaatt tgtggaaaca aagtcggcat gaattctcta tcaaaatcac
     1141 accaaaaaag tccaccaatt ggatgccttc ctccacgagc atacccaaca ctagaaaacc
     1201 aggaatgatt gtaattcttg gccttccaat gatcctcaat cgtgttgaat attttttctg
     1261 gagtctgttc aagatttgaa aattgaacag aaagtccagc gtgagaaata agagtatttc
     1321 ccacaagaaa agcggccccg ataaagtctt cactcataag agtttgaatt ttatcgttta
     1381 tatcaagatt ccacgaaaaa ccagcaaaag aattgctgat gtcaagatat ggaatctcat
     1441 ggttgccgca aatcattgag tcaataatac ttccaacgag atttagacat tcaatatcgc
     1501 cggaaacact gtcatcgaca caatttgcca aatcgcctat agaaacaata aaatgatctg
     1561 ttcctacaat gcgattccca gaactatcaa ccaacttttt cttctcaaga gctcgaaaaa
     1621 gtccgtcaat atttccgtga caatcagaga taatataaat cttcatcatc tctcctttct
     1681 ttttgggctg tctgtatgga gcagactatt taatttctct ctgatgctct gcatttataa
     1741 atttgcgcgg catcctatag aaaacccatt tttccctagt ttctatagag aaaaagcagg
     1801 acggcaaaat tttcgtcttc attttaccgt aatctcttgg cattttcaga gcgccggatt
     1861 tgtctaggaa tctgccaaat tcctgatttg tcccggagga agtaattaac ttccttttac
     1921 ttgataatca agctttttct ctatacaaac tagagaaata gattgaggca cagagattta
     1981 ccggaacccg tactctgttt tccctcggaa aagggcggat gctttattcc gaaaagtttc
     2041 cgctttccgg tcttcgtcat ccctttttta tctagcctca agaaatagtt tagcaaattc
     2101 gtctcactgg acgattttat ttactaaact aaatcttcaa actagccacg tggtttggca
     2161 gccagaatcg ctgccaaaaa acacaaaatg atcaaaatcc agagaaaatt gatcatttta
     2221 tttctttttt cctttctttc tcctttatag gagaagttat gcgaacttga tattccaaag
     2281 aatatctgtt cacatgagaa attgcgtcat cccaatcgaa acaagaccac acaacttccc
     2341 atcctttttc accataaatt tctatggtga aatacttgga gaaatcatct atcatttttt
     2401 ttggcctccc tttttggagt aatttcaaaa ttccgccaaa ggtttttccc ggttttgggt
     2461 atcatggctt tgacggaaat ttcaaattac tttactttca ctagcaagcc ttctgccatt
     2521 atcccttcag cataccattt gtgcggttca ggataatgtg gcccttcgag atacactgtt
     2581 ccattttcag gaacaattcc caaacccggc tgaaataccc ggattttttc tccttcagaa
     2641 agagcagtca taagttcttt cttgctacgg aaatttcttt ccgtataggc catttctctc
     2701 tccttttttt agttataaaa aagaatccgc caccggagac ttatttataa ctaaaaaaaa
     2761 actttggggg gcttagcctc gtctcgtcta ttaccgggat ttgattttag tcccggtaat
     2821 agacgagcat tgtcggattc tttccgcgat ctactgttgc gcctagttct aaccctctct
     2881 tcagatcact ttgggttagt agaatccgac gactaagcgc cggcgctttt cgcgcgggtt
     2941 tcggcttgaa attgccctta ttcccacccg gagtcttagt cggcttcatc gcccgtacca
     3001 tagccgcaga tacagcgcga atgaattcgc ccgctgtgag cggatatcga gcggatatcc
     3061 ttgcagcgct atgacccgac gcgggcggcg atgacgacgt actagtatgt atggatacat
     3121 ttccacaccc cttttcggat taggttagtt cagggtttcg ggtttcgtat tgccccgatt
     3181 ttagttgtct gacttagacg ttttcgtcgt cgttttcgtc gatctcgttt acgatctcgt
     3241 ttacgatctc gttccagtcg ttttcgagtg gtcgcgcaat tgcgaccaca atcgttttct
     3301 cgtcctccgc gaggattgtc tcgcaaagcc cgaagtgttc cagtgccgca cggtacggcc
     3361 cgacctttcc cgtatcctcg ccatttgagc gattgacgct cgttctgagc ataggatact
     3421 cggaagcctt cgtcagcttg gcggaaaacg caatcggatt ctcgtccgac agtagcgcga
     3481 aacctgcacg ccaggccttc tcgacatcgg acgatccctt ctgtcggcct cgtgccgctt
     3541 tgacgcgcgg cggagcgttc tggatcatgt ccagcgtaat tgtctgaagt tccacggtaa
     3601 ccccttttcg tgaggaatcg gggcaatacg tgaaaccctg aactaaccta accttacttt
     3661 tcgacgcgcc aacattactc ggctaacgtc tacctaagcc caccactagc accctaaaac
     3721 cctcgcacgc cgcgccgaaa gtgtgaaccg ttcacctaga cccttcccta gcagcggcta
     3781 ctagtggctt gcagcgtgtc cctatcggct tctcgtctct agaggggtag atgtcgatta
     3841 cctgacaccc tcctctagct tccgcccgcg agactggcaa ctacctacca ctcgcctact
     3901 gctagttcgg aacccctgat ttatggatgc gctaggggta cgtctccgat tactggcaaa
     3961 ggggacggta caggtagcta tgtgaacgtg cggttacgat tgtacggata ttgtaaggat
     4021 cacggggaag ggttctaggc tgtgggacgc tcgcccaccc ttcagcttca caaacccgcc
     4081 taggatcgag tctaagacaa gccggcctag atccgatagc accagacccc taattccatc
     4141 ctggtcttag aagggctcag agtgccccct atcgattcct acgcggagct tagaggggta
     4201 tgtatgtcag gattgtagga ggttcatcca gttgcgccaa acttgaactt aacatataat
     4261 tatgctacag tagagtaatg agtatgcagt tagtagggga gtctttgcct aatagtcaga
     4321 gacatgaatc tttgttgcgc cggatttctg gaactatgtc tatgaacgat agatgtaaaa
     4381 aatgcaaaga attgccttat ccacgcgggt tttttcagaa agaacaatta tgctggtggt
     4441 gtatgtttcc tcgtccgtag gtagttcatg tagttgcgcc aaatttaagc ttaacttata
     4501 attattatag tttttgctat agtagcacac atgaccgaat tagaagaaaa aatatataag
     4561 gatttagaaa atcaatattt cagcgacgaa aaatcaaaga tttgtaatgt ttgtccacaa
     4621 tgcaaggcga ttaaaatgtt tgcgtcgtgt aatcatgggg atgattggcc gagatataca
     4681 gatacagaaa aaattgaagt ttttcctggc gatattgtgt ggtcagaaga tgaatacaat
     4741 tataattctt ttacgcctta ttatgaaaaa gggaaaggaa gagtttattc ttatcaatag
     4801 aatgaaacga ataattgata gggtatgaca aaaagagaat caacttatac tgtagaatgg
     4861 atacgttcta tattgccaga tgagattaat acttgttccg gacttaccgc tagagtggct
     4921 tttgctagat taatacaggc tttaaataat tctgggccga taattgtaaa aaaaataaaa
     4981 attgaaagaa aatgagacga tatttagaca atactataga aggtaattat aaaggacgta
     5041 aacttaggaa cgggaaacgg attataatga ggccggggaa attttggatt ccaatgatta
     5101 gagttggtat gggtagagat tggtataaac ctagaagagc tccaacccgg cgcggagaaa
     5161 aaagaggaca gagtaaacct agatgaattt aagtatctga tggatcatct gtttgtgtga
     5221 ataccggcgg tactttacaa aatggatcat aatacggtct tatttgatta aaatttttat
     5281 catagtctac ataattagtt aatttataat gtaaagctaa agctaggggc cataaatctt
     5341 ctataatttc atttgtcgcg ccaaatttct gtgctgtata gcgccaatgt ctaattgctt
     5401 tacattcgac tttggcttcg tctcccgctc ctccccatcg acgcaaatga taagattcat
     5461 gagttaaagc aagaactcca agagctcttt gccacttaag aatatttgtg tcattgatat
     5521 tttgcgcccc ttcgcataaa cgaaaatcca aagcttcata tgattgtttt cctaaaggct
     5581 tatctacata accccaagca ccataagtcc aaggcgcttc gtcttcgtcg tctaaataac
     5641 aaaatacttc gactggccgc attgcaatcc agctagcaac ttcattgagc ttatcatgct
     5701 tgtaataaga ccccgccgtt ggcgcaagaa tcaaaaacgc taatattgtc agcagaaatc
     5761 tcttcattta taaattctat cacgagcctt aacatactaa taatattgaa tgtgctattc
     5821 tagagtgatg aaaaatccaa tttggacatt agaagaaatt gagaagttat caatgttcgg
     5881 cggcaatgtt ggatggtttt atggcttagt taaacatcat gaaactggaa gagtatgggt
     5941 atatgaaatt tttcccggca cgggttatgc cagaactggt tggatttatc atccgaaacc
     6001 tttgtggtgg gtaattaaag atatttggag agcaactaaa cgtgtttcgt agagataatg
     6061 aaggatggta tattacaatt aaatttagtt cacgatattg gctggtaatt ggtagatgga
     6121 acaaataact aatttagaaa tggctgttaa tattgtaaaa ataggagaaa ctccagaaga
     6181 gcgcacaaaa atagttaata aattattaga gggagaagtt gatactgaaa ctgataatgg
     6241 aaaagggctt ttacatcgca gggctctttt aataactgct ttgtcagaac aaaaaatttg
     6301 gcccatgaaa gaaaataaaa atgcaaaatc taacaaatga acaattattg cggcttattg
     6361 aaagaatagg caaggaaatg acttccgcat ctactacacg acacaacgca atggaaaaag
     6421 aaagaatgat tttaatgaag gaagctttta aaagaggact taaaggatac aaactaaaga
     6481 aaaagtactg gaatttttga tttttaataa gttggcgact tcaaaatgac aaaaattaca
     6541 aaaaatacag acttcgtgtg agatgagaca taaaatggaa aaatattcat cacattgtaa
     6601 atattgtggt tgtcatttta aatggtggaa tttatataaa tgtgtagaag taaatgatta
     6661 gaacttgtaa taaatgtggg gccaaagtag ataataaaaa acctgatcca tgctttggaa
     6721 aatatttacc agaaattgct ttttgttgtt gtggtcatgg aaataatact tatgcatatt
     6781 gtgtaggatg ggaaaattgt ttgccaaacg aaggtccaac agattggcgc gaagaaaatg
     6841 atccaaccta ttggtgttta agaggaatag aagctattga atacatggag aatttaaaat
     6901 gaaattaaca aaagaagaat ggttagattt gaataaaaga tttaccaaaa taaatagaga
     6961 atattttgaa gttaccagtt gttgcgcgat ctgtggaaaa tcattttttg atataacagg
     7021 aatgtcggga caagtttcaa gtatggaata tcctggtctc tataatcatt ttcctaattg
     7081 tccaaaatga aatatacatt tgaagcatat aaagaagatg gaagtcctag aaaatcagcc
     7141 gggatgttta gaccgccata tgaagaagtt tttaagacga taaaagatag tgggcaactt
     7201 tatcgctata tgcagggata tgaaattaat attccttata gtagtggaca atttgataaa
     7261 ccgctttacg aggatgaaga tgaatagacc aaatataaaa gaaatagaga aagatttaaa
     7321 atcccatatt acatctatgc gctcatctat ctggcgggat tatgcagact cggtagaaga
     7381 aaatataaat attcttatta attatattga agaattagaa aatagacttt ttattattaa
     7441 ttccagaacc tctcatgaat attggagtca caaaagttaa tgagcgggca ccttgaagtt
     7501 atttgtggac caatgttcag cggtaaaact gaagaaatgc ttagaagact taggcgtgtt
     7561 gaaattgcag ggcaaaaatc tatagtattc aaaccagtaa tagataatag atatagcgtt
     7621 actgaaatcg caagtcatgt tggcaacaaa agagaagctt atgcagtaaa agatatttat
     7681 gctcttttag atattattgc tcaaggaact tatgatgtca tcgcaattga tgaagtacaa
     7741 ttttttgaat ttgaaattgc agttacaata caaggttttc ttgaagctaa taaaagagta
     7801 atagtttctg gccttgattt ggattttcgt aaagaacctt ttggaattgt gccggagcta
     7861 ttagctatgg ctaaatttgt agttaagctt agagcagttt gtcaagtctg cggcgaagac
     7921 gcaatgtata cgcaacgtct tcttaatgga aaacctgcac ctttgtctgg caaaactgtt
     7981 atagtgggcg gacaagaaca atatgaagct cgttgtgaga aatgctggca ggagggatga
     8041 tagaaattat tttgacaata ttaattttat ttggaataat tattattttg ccaccaatga
     8101 ttatgcttgg tgctaaattt ttagatatat attgtgattg gatcggagag aaaattaatt
     8161 tatgataaaa acaaattcta ttctagactt agacgacctc atggacaaga ttgcggcggt
     8221 ggaatggtga gcgcgagcga gaagctgcga acgctggacg ctgagggact gcattcgtgg
     8281 gcaagggcga cgctagagtc agccgcgcta cgtccagcgc aagacggtgc ttggtacaag
     8341 cacgccgcga tcaagcttgc ggaaaagtcc ctcgcggtcg ctgacgccct cccgcagatc
     8401 gtggcggtgg tggaggcggc ggatgaacat tgtctctgct tcggaatcgg aacgatcacg
     8461 atgatggacg gctcacggca gccctgccaa gttcatgccg ccctcgccgc cctcgaagag
     8521 aagctgtcgt gacgcccgcc gaactcaaag cgctcgcgga ggcggctacg ccggggccgt
     8581 ggacatacgt gatcgaagat gaccgcgagg cttatgtatt cgaggttat
//
LOCUS       DATDNZ010000004         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3151077, whole genome shotgun sequence.
ACCESSION   DATDNZ010000004 DATDNZ010000000
VERSION     DATDNZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3151077"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VI432_00140"
     CDS             <1..741
                     /locus_tag="VI432_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY4496548.1"
                     /translation="QLTDTIILASINTKSHKTALISIPRDTYIKLPIVNTSAKLNEAY
                     EVGEHKQPNGGGIALAKLAVEKIAGVNIDYAIVIDFLAFKETVDLLGGIDVNAAKPLH
                     ENLQWGGKDFVVEPGINHMDGDTALFYVRSRFTTSDFDRARRQQEVILAIKQKATSLG
                     FISSPQKLFGVLDILGRHVKTDLTASEISKLYSIGQKIDSFNVRLKIIDTSEDLLIPS
                     YNDAGAYILLPAGNDFSQIHDVVRNILN"
     gene            751..1581
                     /locus_tag="VI432_00145"
     CDS             751..1581
                     /locus_tag="VI432_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4496549.1"
                     /translation="MLSIIITHYKTPHLLKLSLRSIEETCKDMKPEIIVSESEVQDET
                     KEILEHEFPHVKHLAFTENVGYAKLVNGGILNSQGEYILILNADIVLLDNTVEFLVDF
                     LTKHDEVGMVGPKLLNFNDTHQYSCFKFYRPFTVIARRTFLAKTYFGKKEIKSFLMLN
                     ELNKNANKPLTVDWLMGSIICVKRSAVNKVGLLDERFFMYFEDVDWCRRFWENGLQVI
                     YYPKAQAYHYHQKASDAGRGMLDVFFNKQTRIHASSAIKYFKKYRGKSMPALRSQSKI
                     "
     gene            1585..>2737
                     /locus_tag="VI432_00150"
     CDS             1585..>2737
                     /locus_tag="VI432_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4496550.1"
                     /translation="MKLLLKITVPAFLILASIGAGMIMEYYVQPLASFAEKQNHNTVS
                     LDTDKLNNVWSLLNQKYVNPDSLNSRNALFGAIRGMVNAIGDPYTTFFDPQEAKLFLS
                     DAEGSFEGIGAEIGVRRDLITIISPIHGTPADEAGLKAGDIILKIDEKPTASLGLDEA
                     VSLIRGDKGTIVKLLIARDENDPFIKEIIRAQITIPSVALKMDGSIAYLQLFNFTQKS
                     SEEFNAAALQLINNHAQGIILDLRNNPGGYLERSVDIAGWFLARESVVVIEEDKNQQR
                     TYEKSKGSGRLKDIPMIILINEGSAPAAEILAGALSDNLHVRLVGTNSFGKGSVQSLE
                     ELPDDSAVKITVAKWLTPNGASINDQGIKPDIEVKLTQEDEDEKKDPQFE"
BASE COUNT          881 a          521 c          561 g          774 t
ORIGIN      
        1 caactaacag atacgattat tctggcaagt ataaatacta aatcgcataa gacggcatta
       61 atttctattc ctcgcgatac atatattaaa ctgccgatag ttaatacttc ggctaaactt
      121 aatgaagcat atgaggtggg agaacataaa caacccaatg gcggcggcat agctttagct
      181 aaattggccg tcgagaaaat agcaggggtt aatatagact atgcaatagt gattgatttt
      241 ttggccttta aggaaacggt cgatcttttg ggcggaattg atgtgaatgc ggccaaacca
      301 cttcacgaga atttacagtg gggcggcaag gattttgttg tcgaaccggg cataaaccat
      361 atggatggag acacggccct attttacgta cgctcacgtt ttaccacttc tgattttgat
      421 agagccaggc gtcaacaaga agtcatattg gccataaaac aaaaagcgac ctcacttgga
      481 tttatttcaa gtccacaaaa attatttggc gtgcttgata ttctcggccg ccacgtcaaa
      541 actgatttaa ccgcatccga aatttcaaaa ctatattcta tcggtcaaaa aattgactct
      601 tttaatgttc gtctcaagat tattgatacg tcggaagact tacttatacc atcttataat
      661 gatgccggcg cttatatatt attgccggca ggaaacgatt tttcacaaat ccatgacgtt
      721 gttcgcaata tcctcaattg atttataaaa atgctttcaa taataatcac acattataaa
      781 acaccccatc tgctgaagct ctcattacgc tcaatagaag aaacgtgtaa agatatgaag
      841 cccgagatta tagttagcga atctgaagta caagatgaaa ccaaagaaat attggagcat
      901 gaattccctc atgtaaagca tttggctttt actgaaaatg ttggttatgc caaactagta
      961 aacgggggta ttttaaacag tcagggtgaa tacatactaa ttttaaatgc ggacattgta
     1021 ttattagata ataccgttga gtttctagtt gattttttaa ccaaacacga tgaagtcgga
     1081 atggtcggac caaagttgtt gaattttaac gatacgcatc aatattcgtg ctttaaattt
     1141 tacaggccct tcacggttat agctcgaaga acttttttgg ctaaaacata tttcggtaag
     1201 aaagagataa aatcttttct tatgctaaat gagctgaata aaaacgctaa caagccccta
     1261 acggttgatt ggttaatggg atcgatcata tgcgttaagc gttccgctgt aaataaagtt
     1321 ggtctgttag acgaaagatt ctttatgtat tttgaagacg ttgactggtg cagacgcttt
     1381 tgggaaaatg ggttgcaagt tatttattac ccaaaagcac aggcttacca ctaccaccaa
     1441 aaagccagtg atgccggcag gggaatgctg gatgtttttt tcaacaaaca aaccagaata
     1501 cacgcttcca gcgccataaa atacttcaaa aaatatcggg gtaaatccat gccagcttta
     1561 agatcgcaat caaaaatata atttatgaaa ctattgctta aaattaccgt cccggctttt
     1621 ttaattttag ccagcatcgg cgccggtatg atcatggaat attacgtcca accccttgct
     1681 tctttcgcag aaaaacaaaa tcataatacc gtttcgttag ataccgacaa gctgaataat
     1741 gtatggagct tacttaatca aaaatatgtt aatcctgatt ccttgaacag ccgaaatgcc
     1801 ttgtttggtg cgatacgagg tatggttaat gctataggcg acccctacac cacctttttt
     1861 gatcctcaag aagctaaact ttttctatct gatgctgaag gttcttttga aggtattgga
     1921 gccgaaatag gtgtacgcag agatttaatc accatcattt cacccataca tggaactcct
     1981 gctgacgaag ctggactcaa agcaggagat ataatattaa aaatagatga aaaaccaaca
     2041 gcgtctttgg gtttggacga ggccgtatcg ttgatacgcg gcgataaagg aactattgtc
     2101 aaattactta ttgctcgaga tgaaaatgac ccgtttatca aagaaatcat acgcgcgcaa
     2161 attactatac cttctgtagc attaaagatg gacggttcaa ttgcgtatct gcaattattc
     2221 aattttactc agaaatcctc ggaagagttc aatgccgcgg cgctacaact cataaataat
     2281 catgctcaag gtataattct tgatttgagg aataatccag gcggatattt ggaacgatca
     2341 gttgatatag ccggttggtt cctggcgcgc gaatcagttg tggttattga ggaagataaa
     2401 aatcagcaac gaacttatga gaagtcaaag ggcagcggca gattaaagga tatacccatg
     2461 attatcttaa taaacgaggg ctcggctcca gccgcggaaa ttctcgccgg agctctatct
     2521 gataatctac acgttcgttt ggtaggcacg aatagtttcg gtaagggttc agttcaaagc
     2581 cttgaggaat taccagacga ttcagctgtt aagataactg tggccaagtg gcttacacct
     2641 aacggagctt caattaacga ccaggggatt aagccggaca ttgaggtaaa acttacccaa
     2701 gaagacgagg acgaaaagaa agatccgcag tttgaaa
//
LOCUS       DATDNZ010000005         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2371965, whole genome shotgun sequence.
ACCESSION   DATDNZ010000005 DATDNZ010000000
VERSION     DATDNZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371965"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VI432_00155"
     CDS             complement(<1..700)
                     /locus_tag="VI432_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY4496551.1"
                     /translation="MESDKILEVKNLTVDFGDFRALEEISFDVEKDDSLAIIGPNGSG
                     KTVLLKAILGTVKYSGSVKWNKNIKIGYVPQRLEIDRSLPMTVEEFLETKANILKIGL
                     GEIKKLLYLVNLPEDILKRPIGQISSGQFQRALILFALVGDPNVLLFDEPTASVDEPS
                     EEQIYETLHKLQDEKRMTIIIVSHDLSLVYKHSNKVLCINKNKVCFGEPADVLTSKQL
                     YSLYGGEHKYYHHMH"
     gene            727..1092
                     /locus_tag="VI432_00160"
     CDS             727..1092
                     /locus_tag="VI432_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY4496552.1"
                     /translation="MFRHIGKLSAHENLILRLALAFTFLWSGLSSLYSPENWIGFVPD
                     WIELFIAKDIFLTVHSVAEISLGIGFLIGLWTPIFSLIAFLALISILIIAGIDDITFR
                     DVGLVALALVLFLRSLKRV"
     gene            complement(1092..1162)
                     /locus_tag="VI432_00165"
     tRNA            complement(1092..1162)
                     /locus_tag="VI432_00165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Thr,seq:cgt)
     gene            1335..2213
                     /locus_tag="VI432_00170"
     CDS             1335..2213
                     /locus_tag="VI432_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY4496553.1"
                     /translation="MKELEFEIADPDLWRDDRLGAEAKAKEAGTINDMISRFEKIDSI
                     EKLRELELRLAFTNKYDAFSAVVSVFAGVGGEDASDWANMLLDMYTAYADRRGWKVRM
                     IDENVIHINGEFVYGFLRNESGVHRLVRISPYDSKQLRHTSFALVEVVPDLPELAAKD
                     IRIPDEDLKLDFSRAGGPGGQNVNKVETAVRITHMPTGIVASSRAERSQMRNREIAMK
                     FLKARLIKLLEQTRAKEISDLRVKIKPEWGNQIRSYVMHPYKLVKDHRTDVETSNVGG
                     VLEGDIDLFIEAEIGL"
     gene            2268..2945
                     /locus_tag="VI432_00175"
     CDS             2268..2945
                     /locus_tag="VI432_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4496554.1"
                     /translation="MINFQGVYKSYNGEDILKDISFQVDPKEFISIVGKSGAGKSTLI
                     KLLIGEERPTKGRILFGQHDVSRLKTKDLSKLRRHVGTIFQDFKLLPTKNVFENVAFA
                     LEVAGRPEAEIDDLVMEVLDLVGLADKGAHFLHQLSGGERQRVAIARAMVNKPDVIVA
                     DEPTGNLDHINTTGIINLLTKINELGTTVFLATHNKDVVDHLKRRVILVDGGRIIRDE
                     EKGRYFL"
BASE COUNT          890 a          475 c          652 g          965 t
ORIGIN      
        1 cgtgcatgtg gtggtaatat ttgtgttcgc ctccatatag agaatagagt tgttttgagg
       61 ttaaaacatc tgcgggctcg ccgaagcaaa ctttgttttt attaatgcat aacactttat
      121 ttgagtgttt ataaacaagg cttaaatcgt gggatactat aataattgtc attcttttct
      181 catcctgcag tttgtgcaag gtttcatata tttgttcttc gcttggctcg tcaactgacg
      241 cagttggctc gtcaaagagg agaacattag gatctccgac aagggcgaat aaaataagag
      301 ctctttgaaa ttgcccgctt gatatttggc cgatcggcct tttcaaaata tcttctggca
      361 gatttactaa atatagaagt tttttaattt ctcccaagcc tattttcaat atattggctt
      421 tagtctctaa gaattcttct accgtcatcg gaaggctcct gtctatttcc agtctttggg
      481 gaacatatcc tattttaatg tttttattcc attttacgga tccgctatat tttacggttc
      541 ccaaaatcgc ttttaataaa accgttttcc ccgatccatt cgggcctatg atcgctaagc
      601 tgtcgtcttt ctctacatca aaagatattt cttccagagc cctaaaatca ccgaaatcta
      661 cggttaggtt tttaacttct aatattttat cgctttccat tgtttatgat aagataatga
      721 taaagcatgt ttagacatat cggcaaattg tcagcgcatg aaaatttaat tttaaggctt
      781 gctctggctt ttactttttt atggagcgga ttaagcagtc tatattctcc tgaaaattgg
      841 ataggttttg ttcctgattg gatagaacta tttatagcta aagatatatt tttgacagtt
      901 cattctgtgg ctgaaatttc tttgggtata ggctttttga tagggctgtg gactccgatc
      961 ttttcgctta ttgccttttt agcattaatt tctatactta tcattgccgg catagacgac
     1021 attactttta gagatgtcgg gcttgtggca ctcgctctag tattattttt gaggagttta
     1081 aagcgcgttt gagccagatg tgagaatcga actcacgttt atgctttacg aaagcaccgt
     1141 tctgccactg aactaatctg gctatatttt gcgaaattaa tgacttttga cattgtacag
     1201 aaatcaataa attctacaat actaaatcag tataaaaatt ttatttatga agaaacaaga
     1261 taaacaagaa aatctgcaag agttggctcg ccgcttaggc gagcgtcttt gacatagccg
     1321 gtaaaaaaca aaaattgaaa gaacttgaat ttgaaattgc cgatcctgat ttgtggagag
     1381 atgataggct gggagccgag gctaaagcaa aggaggcagg tacgataaac gatatgattt
     1441 ctagatttga gaaaatagat tctatagaaa agttaagaga attagaactc cgtttggctt
     1501 ttactaataa atatgatgct ttctctgccg tggtgtcggt ttttgccgga gtcggaggag
     1561 aagatgcgtc ggactgggct aatatgcttt tggatatgta tactgcttat gcagatagaa
     1621 ggggctggaa agtcagaatg atcgatgaaa acgtcattca tattaatggt gaatttgttt
     1681 atggattttt aagaaatgag tcaggggtgc ataggctggt tagaatctct ccgtatgatt
     1741 ctaaacagct ccggcataca tcgtttgcgt tggttgaggt cgtgccggac ttgcctgaac
     1801 tcgccgctaa agatatcaga atcccagatg aagatttaaa gcttgatttt tcgcgcgctg
     1861 gcggaccggg aggtcagaat gtgaataaag ttgaaaccgc cgttcgtatt actcatatgc
     1921 ccacaggaat agtcgcttca tctcgcgcag aacgttctca gatgagaaat agggagatag
     1981 caatgaaatt tttaaaagcc aggcttatta aattacttga acagacgaga gcaaaagaga
     2041 tctctgattt gagggttaaa attaagccag aatggggaaa tcaaataagg tcttatgtaa
     2101 tgcatcctta taagcttgtt aaagatcata ggacagatgt tgaaacttct aatgtcggcg
     2161 gcgttttgga gggagatata gatcttttta tagaggcaga aataggcctt taagcaagtc
     2221 ttgatagata ctttatatca aataatttat actttatact gttaggcatg attaattttc
     2281 aaggagtcta taaaagctat aatggagaag atatcttgaa ggatatcagt tttcaagttg
     2341 acccaaagga gtttatctca attgttggta aatctggcgc cggcaagtct actcttataa
     2401 aacttctgat aggtgaagaa aggcccacta aaggacgcat acttttcggc caacatgatg
     2461 ttagtagatt gaagaccaaa gatttgtcta aattaagaag gcatgtcgga actatatttc
     2521 aggattttaa attgctgccg actaaaaacg tttttgaaaa tgtagctttc gctcttgagg
     2581 tagctggtag gccagaagca gagattgatg atttggtaat ggaggtttta gacttggttg
     2641 gtcttgcgga taaaggagca cattttttac atcaactttc gggaggagaa aggcagagag
     2701 tcgctatagc tagggcgatg gttaataagc cagatgttat cgtggccgat gaaccaactg
     2761 gaaatttaga tcatataaac acgactggga ttataaatct tttgacgaag atcaatgagc
     2821 ttgggacaac cgtttttctt gcgacacata ataaagatgt agtcgatcat ttaaagagaa
     2881 gggttattct tgttgatggt ggtagaatta taagagacga ggagaagggg agatactttt
     2941 tataagcatt ttgtataaat ttatgaataa aatgaatata at
//
LOCUS       DATDNZ010000006         5988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_28652, whole genome shotgun sequence.
ACCESSION   DATDNZ010000006 DATDNZ010000000
VERSION     DATDNZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28652"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3371
                     /gene="rpoC"
                     /locus_tag="VI432_00180"
     CDS             <1..3371
                     /gene="rpoC"
                     /locus_tag="VI432_00180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY4496555.1"
                     /translation="GKYRKIRYKGVVCDKCGVEVTRSSVRRERMGHIQLAAPVVHPWF
                     LKTRIGLVLDEPSSKLVRVTYYAGYIVTSVNEDRKKAAIEEIEREFKTKNDPSIKTAF
                     NEAKDAVSGLYVGKILGDSEYFSLVKRFGNVFTAAGGGEGIQQALRNLDLKKAIVGIE
                     KELEGTEDSLRTKKLLRRLKLLKSMIKNNIKPEWMVLNVLPVLPPDLRPMVALDGGRY
                     ATSDLNDLYRRVINRNNRLKKLLELKAPDVIVINEKRMLQEAIDALIDNSVASASKPI
                     SGARRPLRSLADMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKLDECGLPKRMALE
                     LFRPFVISEVIRRGLAHNIRNSNRLIEEAPSEVWEILEEVIKTRVVLLNRQPTLHRLS
                     VQAFKPILIEDLVVQIPPMVCSAFNADFDGDQMAVHLPLSEEAQIEVNNLMLSGHHTL
                     LKPASGDPITVPTQDMVLGSYFLTKILADAKGAGMVFATKEELGLAYHNDFIALNTPV
                     KLGKIETTYGRILFNEVLPPDFGFINETLNKKVLAKLISKIINKYSSAETKQILDAIK
                     SFGFKYATKSGISWGMDDLRVPEAKGEILVETEKKVDLVDEQYGDGLLTDGERRARVI
                     EIWAEAHKNLAKNIPNTLPETGSVYMIIDSGARGSWAQPTQMMGMKGLVTNPNGETIE
                     LPIKTSLKEGHTGLEYFISTHGSRKGLVDTALKTAEAGYLTRRLIDVAQDIVVKEEDC
                     KTKDGIILYRKDGVEFGHSFGARVFSRTAIEDIKSGRKVIVKAGEIISHGASEEIEES
                     KLDEVKIRSVITCKTLYGICAACYGLDLGNNQAIKVGEAVGIVAAQSIGEPGTQLTLR
                     TRHAGGVAGRDITSGLPRVEELFEVRPPKGRALISHVNGTVSKIEEKGSLKTIHITVE
                     SKSKSTARKKAKEKIVEYMVVRSVPMFIKVGDKIASGDVLTEGSLDLRELFLLKGKED
                     AYRYIIKEVQRIYVSEGSSVNNKHIEVMIRQLFGRVKITSPGDTDFVVGDVIDKSSFI
                     EVNQEAKDKGKEQAKAEEILLGVTRAALAADGFLAAASFQETARVLINAATEGRVDHL
                     RGLKENAIIGRRLPIGVPVVLPEEEIGEIADKQN"
     gene            3430..3849
                     /locus_tag="VI432_00185"
     CDS             3430..3849
                     /locus_tag="VI432_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY4496556.1"
                     /translation="MEDTNINTYELSFLLRDDKDIDLISVNLSNIGAEISTQGTISEL
                     KLSYPIEKTNNAYFGYVIFKAEKPAIAKLNNALKLEKKILRFLIVTPPPVDPNAPKAE
                     DASGIRQTRSKVVKSELSNKALEEKLAALRGTEEKPE"
     gene            3888..4322
                     /locus_tag="VI432_00190"
     CDS             3888..4322
                     /locus_tag="VI432_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4496557.1"
                     /translation="MNLNKVFLIGNLTRDPELKTLPSGTSVVSFGLATNRIWKNQQGE
                     NQQDTQFHNIVAFGRQAEIIKQYTAKGSMIFIEGRIQTQSWEAQDGTKRYKTEIVAER
                     IQLGPRTKGASQAGSGSSAPNKNDMPVIDFDSEDIKAEDLPF"
     gene            4375..4581
                     /gene="rpsR"
                     /locus_tag="VI432_00195"
     CDS             4375..4581
                     /gene="rpsR"
                     /locus_tag="VI432_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY4496558.1"
                     /translation="MRKEQCFFCSQNAVDIDYKETDLIRRFVSSQAKIIDPIHTGTCG
                     KHQRKLARAIKRARFMGLLPYVRQ"
     gene            4594..5124
                     /locus_tag="VI432_00200"
     CDS             4594..5124
                     /locus_tag="VI432_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4496559.1"
                     /translation="MRLDESIFDKIYSLSGQNPFLDGLFIFFADYLIYAVMLIFFWLV
                     FFRLKDTKDRAYFFYLGVLSVIFSWGIVTEIFRFFYYSPRPFVLEFESLIRHIDTSSF
                     PSGHMMFIIPIVLSVFYIDKKWGLILLSMAILTAISRIAVGVHWPIDIIGAFFLGPAS
                     FYSIKYLYDLKLRKRK"
     gene            complement(5151..5753)
                     /locus_tag="VI432_00205"
     CDS             complement(5151..5753)
                     /locus_tag="VI432_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496560.1"
                     /translation="MGNADTVLRLKKSAAENNGCEKWNAWWIEILIELNGREKVLSLI
                     KNPSGTIVRENINWDKFMELLNSQNIIAPKIDIRGGNFLSLSLEGAILVGARANEASF
                     RDMAHAICIGMEAPESSWRKANGADLRLAVIKSAVFLGDNIGMRTDGAKMDFADFSRV
                     NDPPADLPETAIAPPWLNGTKSREPQGYGTRDEITNSRFH"
     gene            complement(5913..5987)
                     /locus_tag="VI432_00210"
     tRNA            complement(5913..5987)
                     /locus_tag="VI432_00210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5960..5962),aa:Leu,seq:caa)
BASE COUNT         1799 a         1011 c         1397 g         1781 t
ORIGIN      
        1 gcggaaaata tagaaaaata agatataaag gagttgtttg cgataaatgc ggagttgaag
       61 tcacaaggtc gtctgtcagg cgggagagaa tggggcatat tcagctagct gcgccggttg
      121 ttcatccgtg gtttctgaaa acaaggatag gtttggtttt ggacgaaccg tcttctaaat
      181 tagtaagagt cacttattat gctggttata ttgtgacttc tgtaaacgaa gatagaaaga
      241 aagcggctat tgaagaaata gagagagaat tcaaaactaa aaacgatcca tctatcaaaa
      301 ctgcctttaa tgaggctaag gatgcggtta gcggcttgta tgttggtaag attttagggg
      361 actctgagta tttttcattg gttaaaagat tcggaaatgt ttttacagcc gcaggaggag
      421 gagagggtat tcagcaggct ttgaggaacc tcgatcttaa aaaagcaata gttggtattg
      481 aaaaagaact tgaaggcaca gaggacagtt tgcgaactaa aaaattgctt cgtcgtctaa
      541 agcttttaaa gtctatgatt aagaacaata ttaaacctga atggatggtg ttgaatgttc
      601 tgcctgtttt accgccagac ttaaggccta tggtcgcttt agacggaggt agatatgcga
      661 catcagacct gaatgattta tacagaagag ttataaacag gaataaccgt ctaaaaaaat
      721 tattagagct taaggcgcct gatgtaattg tgattaatga aaagagaatg cttcaagagg
      781 ctatagatgc tttaatagat aattctgtcg catcggcttc gaaaccaata agcggagcga
      841 gaaggcctct tagatcttta gctgacatgc taaagggaaa gcagggcaga tttcgtcaga
      901 atttgctcgg aaaaagagtt gattattctg gaaggtccgt tatcgttgtc ggtcccaatt
      961 tgaaattaga tgagtgcgga ttgcccaagc gaatggcgct agagttgttc cgcccgtttg
     1021 ttataagtga agttattaga aggggattgg ctcacaatat aaggaattcc aatcgcctga
     1081 ttgaggaagc tccatctgag gtatgggaga tacttgaaga agtaataaaa acgagagtcg
     1141 tgctcttaaa taggcaacct accttgcata gattaagcgt tcaggcattc aagccgatcc
     1201 ttatagagga tttggtcgtt cagattcctc cgatggtttg tagcgcattt aatgccgatt
     1261 tcgatggaga ccagatggcc gttcatttgc ctctttctga agaggctcaa atagaggtta
     1321 ataatttaat gctctctggg catcataccc ttttgaagcc cgcctcagga gatccgataa
     1381 cagttcctac tcaagatatg gttttaggat cttatttctt aacaaaaatt cttgctgatg
     1441 ctaaaggcgc aggtatggtt tttgcgacga aagaagaatt aggccttgct tatcataatg
     1501 attttatagc gttgaatact ccggttaagc ttgggaaaat tgaaaccact tatggtagga
     1561 ttcttttcaa tgaagttttg cctcccgact tcggctttat caatgaaacg ttgaataaaa
     1621 aggttttagc taaactaatt agtaaaataa taaacaaata tagttcggca gagacaaagc
     1681 agattctaga tgcaattaaa tcattcggtt tcaagtatgc tacaaagtcc ggcattagct
     1741 ggggaatgga tgacttaagg gtgccagaag cgaaaggtga gatcttggtt gagactgaga
     1801 aaaaagttga tttggttgat gagcagtatg gcgacgggct tctaactgac ggagagagaa
     1861 gggcgagggt tattgagatc tgggctgagg cgcataaaaa ccttgctaaa aatattccga
     1921 atactcttcc agagacggga tctgtttaca tgattattga ttctggagca agaggaagtt
     1981 gggctcagcc tactcagatg atgggtatga agggtttggt gaccaatcct aatggagaga
     2041 cgattgaatt gccgattaag acctctttaa aagagggtca tacgggtttg gaatatttta
     2101 tttcaactca cggctctaga aagggtcttg tcgataccgc tcttaaaaca gctgaggcag
     2161 gatacttaac gaggagactt attgatgtgg ctcaagatat cgttgtcaaa gaagaggatt
     2221 gtaagactaa agatggaata attttatata ggaaagacgg cgttgagttt ggtcattcgt
     2281 tcggagcaag agtgttttca agaaccgcta ttgaagatat taaatccggc aggaaggtta
     2341 tagttaaggc tggagagata atatctcatg gagcatctga ggagattgaa gaatcaaagt
     2401 tggatgaagt taagataaga tcagttatta cttgcaaaac attatatgga atttgcgcgg
     2461 cttgttatgg cttggatcta ggcaataatc aggctattaa agttggtgaa gctgttggaa
     2521 ttgttgccgc tcagtcaatt ggggagccgg gtacgcaact tacgcttcgt acgcgtcacg
     2581 ccggaggtgt tgctgggcga gatattacat ctggtcttcc tagggtagaa gagttgtttg
     2641 aggtgcgtcc gccaaaaggc agagcgctta tttctcacgt gaatggaacg gtttctaaga
     2701 ttgaagaaaa aggatctcta aaaactattc atattaccgt tgagagtaaa tcaaaatcaa
     2761 ctgctcgcaa gaaagctaaa gaaaaaattg ttgaatatat ggttgtaagg agcgtgccaa
     2821 tgtttatcaa agttggagat aaaatcgcaa gtggagacgt tttaacagag ggaagtttgg
     2881 atttgagaga gctcttcctg ctaaaaggaa aagaagacgc ttaccgttac ataatcaaag
     2941 aagtccaaag aatctatgtg tctgaaggaa gttcggtcaa taataaacac attgaggtta
     3001 tgattaggca gctgtttgga agagttaaga taacatctcc tggagataca gattttgttg
     3061 ttggagacgt tatcgataaa tcaagtttca tagaggttaa tcaggaggcg aaagataaag
     3121 gcaaagagca agcaaaggca gaagaaatat tacttggagt tacaagagcg gctttagctg
     3181 cagatggctt tttggcggct gcctcttttc aagagacggc cagagttttg attaatgctg
     3241 ctactgaagg aagagttgac catttaagag gcttaaaaga gaatgcgatt attggaagga
     3301 ggttgccaat tggtgtgcca gtcgtgttgc ccgaagagga gattggagag attgctgata
     3361 aacaaaattg acacccgtag caagcgcatg ttaatctaga ccagttttta attagttaaa
     3421 ttattcgcaa tggaagatac aaatataaat acatatgaac tttcgttcct gcttagggat
     3481 gataaagata tagatctgat ttcagttaat ctatctaata tcggcgcaga gatttcaact
     3541 cagggaacga tttcagagct gaagttgagt tatccgatag agaaaacaaa taatgcctat
     3601 tttgggtacg ttatttttaa agcggagaag ccggcgatag ctaaattaaa taatgcattg
     3661 aagctcgaaa agaaaattct tcgttttctt attgtcactc ctccgccagt cgatcctaat
     3721 gctccaaaag cagaggatgc ttcgggaata agacagacta gatctaaagt cgtaaaatca
     3781 gagctttcta ataaagccct tgaagaaaaa ttagccgcgc ttagagggac cgaagaaaaa
     3841 cccgaatagc attgaataat tacttttaag ttatagaatt aaaaataatg aatctcaaca
     3901 aagttttctt aataggtaat ttgacgagag acccggaatt aaaaaccctt ccttctggaa
     3961 cgtctgtcgt atcttttggt ttggcgacta ataggatctg gaagaatcag caaggagaaa
     4021 accaacaaga tacgcagttc cataatatag tcgctttcgg taggcaggct gagattatca
     4081 agcagtacac cgctaaaggc agcatgattt ttatagaggg caggattcag actcaatcat
     4141 gggaggcgca agacggcacc aaacgttata aaactgaaat cgttgccgaa agaattcagt
     4201 tgggcccgag gactaaaggc gcatctcaag ctggtagcgg atcttccgct ccaaataaaa
     4261 acgatatgcc tgttatagat ttcgattcag aggatatcaa ggccgaagac ttgccatttt
     4321 agatataaat aactaatatt gttagattat taaattatca tgattagtaa caaaatgaga
     4381 aaagagcagt gttttttctg ctcacagaac gcggttgaca ttgattataa agaaactgat
     4441 ttaatcaggc gatttgtttc cagccaggct aaaattattg acccaataca tactggtact
     4501 tgtggtaagc atcaaagaaa attagcgaga gcgataaaga gagcgcgatt catgggtctt
     4561 cttccatatg ttaggcaata acattttttt gttgtgcgtt tagacgaatc cattttcgat
     4621 aaaatttata gcttgtcggg acaaaacccg tttttagacg gtctgtttat tttttttgcg
     4681 gattacctta tttatgctgt tatgcttata tttttctggc ttgtattttt taggcttaaa
     4741 gacacaaaag atagggcgta ttttttttat ttaggtgttc tctccgttat attttcatgg
     4801 ggtatcgtca cagagatatt tagatttttt tattactcgc cacgcccatt cgttttagaa
     4861 tttgaatctt taatcaggca tattgataca tcatcatttc catctggaca tatgatgttt
     4921 attatcccga tcgttttaag cgttttttat atagataaaa aatggggatt aatactctta
     4981 tcgatggcca ttttaacagc tatttctaga atcgctgtcg gggtgcattg gcccattgat
     5041 attatcggag cgtttttctt ggggccggca tctttctatt ccattaaata tctatacgat
     5101 ttaaaattaa gaaaaagaaa atagcgcctt taatcaggcg ctgtttttgc ttagtggaaa
     5161 cgagagttag ttatctcatc tcgtgtccca tatccttgtg gttcgcggct cttcgtccca
     5221 tttaaccatg gcggcgcgat agctgtttcg ggaagatctg ctggaggatc atttaccctg
     5281 ctgaagtcgg cgaaatccat ttttgctcca tctgtgcgca ttccgatatt gtctcccaag
     5341 aagactgctg atttgattac tgctaggcgg agatcggctc catttgcttt gcgccaggaa
     5401 gattcgggtg cttccatgcc gatgcagatt gcgtgggcca tatctctgaa gcttgcttcg
     5461 ttagctcttg ctccgacaag aatcgccccc tcaagagaga ggctaaggaa attgccaccc
     5521 ctaatgtcga ttttaggagc tatgatgttt tgggaattga gaagttccat aaacttatcc
     5581 cagttgatat tctctcttac gatcgttccc gaagggtttt ttatcagtga taatactttt
     5641 tcgcgtccgt tcagttcaat aagaatttcg atccaccatg cgttccattt ttcgcagcca
     5701 ttattttctg ctgctgactt tttcagtctt aacactgtgt ctgcattccc catttatctt
     5761 tcctccgtct ctccttattt ctcacaagga gtttcgaggt actgggatta agcttaacaa
     5821 agaggatttt tatgtcaaac aagataaatt ctttggctca ggatatgctt tgaatacagc
     5881 gatattgtta tttattcgat tcgcgtcttt tgtggccagg gagggacttg aaccctcacg
     5941 atatcgctat cacaggattt tgagtcctgc gtgtctgcca ttccacca
//
LOCUS       DATDNZ010000007         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3172589, whole genome shotgun sequence.
ACCESSION   DATDNZ010000007 DATDNZ010000000
VERSION     DATDNZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3172589"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..327
                     /locus_tag="VI432_00215"
     CDS             124..327
                     /locus_tag="VI432_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496561.1"
                     /translation="MKQCLICHKGSRMVGKRIKLRGNYNPTGMTRKYPNLQKTTLSTG
                     ERVLACVRCIKTITKKAVGSMVN"
     gene            458..1075
                     /locus_tag="VI432_00220"
     CDS             458..1075
                     /locus_tag="VI432_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY4496562.1"
                     /translation="MLYQPQKFDNLLGTPGFSDLLLKNHFTLYEGYVKNINKLVDDLK
                     EAERGGWLGTPGYAELKRRFGWEWNGMRLHELYFGNMSKTPTRLEESKIPSKVEGTKK
                     ATLTEEINRVYGSYENWEKDFKAVGAMRGIGWTILYQDTVSKELFNVWVNEHDMGHLA
                     GCKPMLVLDVFEHAYIGDYGLRRADYIEAFFRAIDWLVLEGRFDK"
     gene            1191..2108
                     /locus_tag="VI432_00225"
     CDS             1191..2108
                     /locus_tag="VI432_00225"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY4496563.1"
                     /translation="MHLLLFIAISALVGGGASVAAEGSLPGDVLYPIKVNVNERVRGF
                     IALSEEAKADLNSNLAVRRLDEVERLAVRGDLSADVRTRLESNFEAFADRVETRIKDL
                     EDAGKINAALDVGSRFESSLSAHERILAEINVSRPAISSILGKVRTEINNSANARARV
                     ESKIGASASADVQAAAEGRLKAAENKITEARAYIEAKKVEISAEAYAEAQARLNSAES
                     RIASGKISFEAGVYADAFLSFQAAHRQAQEAKLAVQVRSGLLKIEVSNNTSENTNNGE
                     ANVNANTEINVNSSGDVNVEGEVEVEIGL"
     gene            complement(2162..2602)
                     /locus_tag="VI432_00230"
     CDS             complement(2162..2602)
                     /locus_tag="VI432_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496564.1"
                     /translation="MNKNLIVIFGVIIIIFTILFFGFNNSSETNANPNLSETNNGYTE
                     IEPINLDDPSAFIVNITEENGYEIKDFTIKKGQTVVFKNLTADGNFWPASNIHPTHQI
                     YPEFDPKEPIPPAGEWGLKFNNAGIWRWHDHLRPSIKGIITVEE"
BASE COUNT          925 a          442 c          594 g          819 t
ORIGIN      
        1 aagagtaact atctgaaata ttagaattaa tatattatcc attattcttg ctttctaatt
       61 cgtttattga tactatacta cactacgaac taatcgctag tcgtataaaa tattattatt
      121 ttaatgaaac agtgtttaat ctgccacaaa ggatctcgaa tggtcggcaa acgaattaag
      181 cttaggggta attataaccc aaccggcatg acgagaaaat atcccaattt gcaaaaaact
      241 accctttcta caggagagcg tgttctggct tgcgtaagat gcattaagac gataactaaa
      301 aaagctgttg gttcgatggt caattagaat aaaagccatt atttaactaa ctattcgggc
      361 tgtcgtataa aagcagtatg catgcatggg gtgcatgcgg ccccggagca ttaccgggca
      421 gcccgacaga ttaaatctta tttggtataa ttcttttatg ttatatcaac cgcaaaaatt
      481 tgataatcta ttgggaacac cgggctttag cgatctccta ttgaaaaatc acttcactct
      541 ttatgagggt tatgtaaaga atattaataa attagtagac gacctaaaag aagcagaaag
      601 aggaggttgg ttaggcactc ctggttatgc agaactcaag cgtcgatttg gttgggaatg
      661 gaatgggatg agacttcacg aactttattt tggaaatatg tcaaagactc caacaaggct
      721 ggaagagtcg aagatcccga gcaaagtcga gggaacaaaa aaagcaaccc tcaccgaaga
      781 gatcaatcgt gtttacggat catatgaaaa ttgggaaaag gatttcaagg cggtaggagc
      841 aatgcgcgga attggctgga caattttata tcaggatact gtgagtaaag aattatttaa
      901 tgtttgggtg aacgagcatg atatggggca tcttgccgga tgcaaaccta tgctcgtact
      961 tgatgttttt gagcacgcct atattggaga ttacggattg aggcgcgcgg actatataga
     1021 agcgtttttc agagcaattg attggcttgt cttagagggc agatttgata agtaagatta
     1081 ctcttattgt cgcgtctttt attacgaaac aattataaat ggaaaaacca ttttaatgtg
     1141 gtagtataaa aagtattaaa aggtcaaaaa ataaaaatta aaaataaaaa atgcatctat
     1201 tgttattcat agcgatttca gcattggtgg gaggaggggc aagcgttgcc gctgagggtt
     1261 ctcttcctgg agatgtttta tatcctataa aagtcaatgt taacgagaga gtaagaggtt
     1321 ttatagcttt atctgaagaa gctaaagctg atttaaactc aaatttagca gtaagacggt
     1381 tagatgaagt agaaaggctt gcggtacgtg gcgatttgag cgcagatgtc agaacgcgtc
     1441 tagagtctaa ttttgaggct ttcgctgata gagttgaaac cagaataaaa gacttggagg
     1501 acgccggtaa aattaacgct gcgttggatg tcggttcaag atttgaatca tcgttgtccg
     1561 cgcatgaaag gatcttagct gaaataaatg tttcaagacc ggctataagc agcatactgg
     1621 gaaaagtaag aacagaaata aataattcgg caaacgcgag agccagagtt gaatctaaga
     1681 taggcgcaag cgcatcagct gatgttcaag cggccgcaga aggaagatta aaagcggcag
     1741 agaacaaaat aactgaagct agagcttata tagaagctaa aaaagttgaa ataagcgctg
     1801 aagcttacgc tgaagctcaa gcaagattaa attcagcaga gagtagaatt gcatcaggca
     1861 agataagttt tgaagccgga gtttacgccg acgcattctt atcttttcaa gctgctcata
     1921 gacaagccca agaagctaaa ttagcagtac aagtaagatc aggccttcta aaaattgaag
     1981 taagtaataa tacttcagaa aacacgaata acggagaagc caatgttaac gctaatacag
     2041 aaattaatgt taattcttct ggcgacgtta acgtagaggg cgaggttgaa gtggaaatag
     2101 gattataatt aaatttaaat ataaaaatac cccgctaata gccgggtatt tttatattgt
     2161 tttattcttc aactgttata atgcctttaa tagaaggcct taaatgatcg tgccatcgcc
     2221 atatcccagc attgttaaat tttaaccccc attctccagc gggaggaatt ggttctttag
     2281 ggtcaaattc gggatatatt tggtgagtag ggtgtatatt tgaagctggc caaaagtttc
     2341 cgtcagcggt taagttttta aatacaaccg tctggccctt tttaattgta aaatctttaa
     2401 tctcatatcc attttcttcg gttatgttaa caatgaatgc gcttggatca tcaagattta
     2461 tgggttctat ttctgtatat ccattatttg tttcagataa atttggattt gcattggtct
     2521 cagaactatt attaaagccg aaaaacaaaa ttgtgaagat aatgattatt acaccgaata
     2581 ttacgataag atttttattc atgttttgaa ttatacataa attatattga tggacaagta
     2641 tatctaaact cttcttcaaa tgtagagcaa acctcgtttc ttgcctgatc tgaagcgaat
     2701 aattctttca atctgcctcc caaagaatta taacaggtcg ttttatgttt gttgtcagaa
     2761 atcaatttgc ataattccgt
//
LOCUS       DATDNZ010000008         9878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2910178, whole genome shotgun sequence.
ACCESSION   DATDNZ010000008 DATDNZ010000000
VERSION     DATDNZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910178"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VI432_00235"
     CDS             complement(<1..773)
                     /locus_tag="VI432_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY4496565.1"
                     /translation="MIDINIIRENPNLVREACKKKKSDPSLVDKFLDLDKEWRALVGD
                     TDVLRAKKNNLGKDNIDEAKKIKDNLKEIEARLKVVENERDMVIEQIPNIPFDDVPEG
                     RDENDNVVLREIGDKRNFSDEGFEPGDYLSLAGPLINTDKASEVAGSRFGYLLGDLVL
                     LEFALVRLAFEKLLPHGFIPVIPPVMARPDILKAMGKIKFMEGDDAFYLPKDDLYLVG
                     SSEHTIGPIHIGETIEAKKPPIRYIGFSTCFRREAGSYG"
     gene            complement(868..2322)
                     /gene="lysS"
                     /locus_tag="VI432_00240"
     CDS             complement(868..2322)
                     /gene="lysS"
                     /locus_tag="VI432_00240"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY4496566.1"
                     /translation="MIEVLREERLRKLKNIKDRGINPYPASAKRSRVISDALKSFSSL
                     SAKKTKIYLVGRIRSIRDQGGILFMDLQDDTASIQLVVKKEDIGDDFIFWRDNLDIGD
                     FLQVRGYLLETKRGEKSLGVLSITILSKSLLPIPSDFYGIHDEETRLRKRYLDILVNK
                     DAKEIFIQRAKFWEVTRKFLRSQNFLEVETPILEVTTGGAEATPFKTHHNDYDMDVYL
                     RVSVGELWQKRLMAAGFGKTFEIGRIFRNEGSSPEHLQEFTNLEFYLAYADFEDGIAF
                     TEKMIKEVVEETFGKTAFSTRGFSFDLSGNWPRIDYVETVEKMTGINVLKATENEMMN
                     KLKDLGVSFEGSNRERLTDTLWKYCRKQIAGPTWLINHPKLVSPLAKVNQDNPDLTLR
                     VQLILAGAEVTNGFAELNDPIDQCERFDLQKKLIEKGDVEAMMPDDEFVEMLEHGMPP
                     TFGFAYGERLFAFLVDKPLRETQLFPLMKPRKNT"
     gene            complement(2419..2877)
                     /gene="greA"
                     /locus_tag="VI432_00245"
     CDS             complement(2419..2877)
                     /gene="greA"
                     /locus_tag="VI432_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4496567.1"
                     /translation="MNYFLTEDRLAELKTELSSLKTVKRIEISERLKRAKELGDLSEN
                     AEYLEAKDEQERVERRIDDLEGIVQNATIIKKNIDKTIIDIGSTVEVLRDGKTMKFFI
                     VGSNEARPEEGRISNESPLGRELINKKVGDSVTVETPQAKKIQYKIFKIT"
     gene            complement(2919..3350)
                     /gene="rpsI"
                     /locus_tag="VI432_00250"
     CDS             complement(2919..3350)
                     /gene="rpsI"
                     /locus_tag="VI432_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4496568.1"
                     /translation="MITKETKNSKNYIEAVGRRKSATVRVRIYPTSAPQDIKGKFVIN
                     DKDIKNYITTLRYREKALAPLKLIGEDAVKAVSVKAVGGGIMAQADAICLGLARAMTK
                     LDGDLKKKFRAHGYLTRDPRAVERKKYGHSKARRGHQWRKR"
     gene            complement(3377..3718)
                     /gene="rplM"
                     /locus_tag="VI432_00255"
     CDS             complement(3377..3718)
                     /gene="rplM"
                     /locus_tag="VI432_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4496569.1"
                     /translation="MEYVIDAKGKTLGRLATKIAGILQGKTDAKYAPRKRGDNTVVIK
                     NISLLKVTGNKAQQKIYYRHTGYVGHLKERRYEEAFSRSPEWVLKHAVAGMLPKNALR
                     QQRLKMLKVEK"
     gene            complement(3723..4076)
                     /gene="rplQ"
                     /locus_tag="VI432_00260"
     CDS             complement(3723..4076)
                     /gene="rplQ"
                     /locus_tag="VI432_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY4496570.1"
                     /translation="MRHLNKGRKFGRVKGRRKSFINGLLHNFFIEEKIKTTEARAKEI
                     KPKAEKLITLAKKNDLASLRLLISRMPKKTAFKLYYDIAPRYKDRRGGYSRIIKMSAS
                     RKRDAAPQVILELVK"
     gene            complement(4079..4816)
                     /gene="rpoA"
                     /locus_tag="VI432_00265"
     CDS             complement(4079..4816)
                     /gene="rpoA"
                     /locus_tag="VI432_00265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY4496571.1"
                     /translation="MEFVYLSESVKINRVSEDDKKGVFDIDGLYRGYGITIGNAIRRV
                     LLSSLPGAAITRFKIKGVGHEFTTIPGVVEDVLEIGLNLKKVRFAFHAKEPQVLTLKV
                     KGEKEITAADIKGNADVEVKTPEVHIATITDKSAELDIEITVEKGLGYVPVISQKAGK
                     LPIGTVALDAIFSPVINANFTVSNMRVGERIDYNKIRLEVETDGTISPSRAVHKAADI
                     LLDHFKRISDIEIKDAAEGAEKNEDKK"
     gene            complement(4848..5459)
                     /gene="rpsD"
                     /locus_tag="VI432_00270"
     CDS             complement(4848..5459)
                     /gene="rpsD"
                     /locus_tag="VI432_00270"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4496572.1"
                     /translation="MHKILEKRERSLGAKLSIKGHRCNSPKCALIRKPYRPGMHGKNR
                     HKIGEFGTQLQEKQKIRFTYGLTDKQLRGIFRRAEKMTDPTPKIVMEMLESRLDNVIF
                     RLGFSESRIAARKMVSHGHFIVNGKRVTIPSYSVKEGDIINIRPASKEIGQLKDIAEK
                     LKNYEAPAWLLLDKEKLEGKVLSTPLDIDSSFDVSQVVDYYLR"
     gene            complement(5483..5935)
                     /gene="rpsK"
                     /locus_tag="VI432_00275"
     CDS             complement(5483..5935)
                     /gene="rpsK"
                     /locus_tag="VI432_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY4496573.1"
                     /translation="MGKNKIIEKKPDITVKTSASKSDESGDKVVKVQGKKIQNGRIYI
                     NASYNNTVVTATNEKGDVLAWATAGSLGFSGPKKATPFAASKIIAAIAEKISKTGPQD
                     VDVFVSGVGSGRDSALRSLINHGFNILSIRDVTPVPHNGPKPPKVRRV"
     gene            complement(5950..6336)
                     /gene="rpsM"
                     /locus_tag="VI432_00280"
     CDS             complement(5950..6336)
                     /gene="rpsM"
                     /locus_tag="VI432_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY4496574.1"
                     /translation="MMRLAGINIPDEKRIEISLTYVYGVGRSMAKNILTAAQIDLGKK
                     AKDLTAEEINSIKDVIEKKYKIEGELRQVIKQNIARLKDIKSYRGIRHMRKLPVRGQR
                     TKTNSRTVRGNVRKTAGSGRRKVELK"
     gene            complement(6442..6555)
                     /gene="rpmJ"
                     /locus_tag="VI432_00285"
     CDS             complement(6442..6555)
                     /gene="rpmJ"
                     /locus_tag="VI432_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY4496575.1"
                     /translation="MKVRASVKKICKDCKTVRRKGRLYIVCVNPKHKQRQG"
     gene            complement(6593..6817)
                     /gene="infA"
                     /locus_tag="VI432_00290"
     CDS             complement(6593..6817)
                     /gene="infA"
                     /locus_tag="VI432_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY4496576.1"
                     /translation="MASSPGKNTTTLQGMVVEALPATTFKVKLDDNREILAYASGKIR
                     MHHIKILPGDRVLVEMGAYDDKRGRITRRL"
     gene            complement(6900..8516)
                     /locus_tag="VI432_00295"
     CDS             complement(6900..8516)
                     /locus_tag="VI432_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496577.1"
                     /translation="MAKKIYIDKHDSISSIIERILNISERELIIYIPRFTESLSTINN
                     LKLLKRETEAAGKKIYIESVDDKILKIAASLNMKATNPFFQKRSKPVSDILSPNTPII
                     LDEDEDKAEADTFSEEEEKDWLKEKIKVRKEKRKMPKFTTPQITIKRPMFLVGISIFI
                     LGILGVAIFVLPRAHITLIFDKLNWEFEGSLLISPSIAKSEFSMNQLKVSGALFEDQD
                     LLEKSYPATGVDTVERKAEGKLTIFNAYSSDSQPLVKNTRFVTPDGKIFRIYNDIVVP
                     GAKIVDSKIVPSTIEVQVMADQTGTEYNIAKSKFRIPGFQGSPKYEGFYAESDMPMSG
                     GFIGEVKIPTSEDIKNAKSNLRTELENSLRSAFLSNAPNDIKILDKAIQFSIVEEVVD
                     DMVDEGGNFKISVTAKIKAVGFKEADLISVLREKLLSEAEGDESINNNSVLEGGNIDI
                     ELINRNIEYGEPRIDFIKGEMSLSVQFDSVWTRALNLTQFRENIRGESKKEVEELISS
                     IPGLKARVSLWPIWVFSIPDKPSKIDIDVE"
     gene            complement(8526..>9878)
                     /locus_tag="VI432_00300"
     CDS             complement(8526..>9878)
                     /locus_tag="VI432_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY4496578.1"
                     /translation="GVPKDKESKSIWNELGVKDVREEGIEDVFWGPTGNEGPCGSTTE
                     IYCKNASGKDVEIWNIVFNQFFYPGSREELLAETPGKVLKPLETPGVDTGMGLERLVM
                     IAQNAKNIFETDLFQLIIDLLPAELDTSKKRIIADHVRAISFLISDGVRPSNKGASYI
                     LRRLMRRLIVASQGMEMKNIFETIVKNYSGAYPELNIQNILSVFGEEKDRFEKILKEG
                     EREIAKSDSIDVKLAFKFYESYGLPYEVIKDLAGDKVKDLKRADFEREFAHHQEISRA
                     GQEKKFGGHGLILNTGELKAANEEELKKVTRLHTATHLLQAALRKVLGPEVKQMGSDI
                     TAERTRFDFTFPRKLIDEEIKRVEDEVNGAIRRALPMQMKKMKIDEAKQTGALYFFRD
                     KYPNEVNIYFAGNSLEDAFSKEFCGGPHVKNTSEIGEFKIVKEEASSAGVRRIRADVV
                     "
BASE COUNT         2720 a         2265 c         1487 g         3406 t
ORIGIN      
        1 tttccatatg agccagcttc tctcctaaaa caagttgaaa aaccaatata acgaattggc
       61 ggtttttttg cctcgatagt ctcccctata tgaattgggc caatagtatg ctcagatgat
      121 ccgactagat acagatcatc tttaggaaga taaaaagcat catcaccttc cataaattta
      181 atcttaccca ttgcttttaa aatatcgggg cgcgccataa ccggaggaat cacagggata
      241 aatccatgcg gcaataattt ttcaaaagca agacggacaa gggcaaattc aagcaagacc
      301 aaatcgccta aaagataacc gaacctgctt ccagcaacct cagacgcctt atcggtatta
      361 atcaatggtc ctgcaagcga caaataatca cctggctcaa aaccctcatc agaaaaattt
      421 cgtttatcgc cgatctcacg caaaaccaca ttatcattct catctctgcc ctccggcaca
      481 tcatcaaagg gtatattcgg gatctgttca ataaccatat ccctctcgtt ctcaaccacc
      541 ttcaacctag cctcgatctc tttaagatta tcttttatct ttttagcttc atctatattg
      601 tctttgccta aattgttctt cttagctctt aggacatccg tatcgccgac taaagcgcgc
      661 cactctttat ctaaatccaa aaatttatca actagagaag ggtcagactt tttcttcttg
      721 caagcctcac gcaccagatt aggattctca cgaattatat taatatcaat catgcctctg
      781 aaaacatctg acaattgaca tctgacaatt gacctttaat tttaaattct cataaataaa
      841 ttgcattttt atgttaattg ttgcttgtta agtgttcttg cgcggtttca ttagtggaaa
      901 caactgcgtc tctctaaggg gtttatcaac cagaaaagcg aatagtcttt ctccataggc
      961 aaaaccgaaa gtcggaggca taccatgttc aagcatctcg acgaactcat catccggcat
     1021 catagcctcg acatctcctt tctcgataag cttcttttgc aaatcaaatc tttcgcactg
     1081 atcaatagga tcatttaatt ccgcaaaacc gtttgtaact tcagcgccgg caagaataag
     1141 ctgtacgcgt aaagtaagat ctggattatc ctgattaact tttgccagtg gagaaacaag
     1201 cttaggatgg ttaatcaacc atgtgggccc agcaatttgt tttcggcaat atttccaaag
     1261 agtatcagtt aaacgctctc tattacttcc ctcaaaagaa acacctaaat cttttaattt
     1321 attcatcatt tcattctcag ttgcttttaa aacattaatg cccgtcattt tttctaccgt
     1381 ctccacataa tcaatcctcg gccaatttcc cgatagatcg aatgaaaaac cccttgtaga
     1441 gaaagctgtt ttgccgaatg tctcttcaac aacttcttta atcatttttt cagtaaaagc
     1501 aataccatct tcaaagtctg cataagccaa ataaaattct aagtttgtaa attcctgtag
     1561 atgttctgga ctagagcctt cgtttctaaa gattctccct atctcaaatg tcttgccgaa
     1621 accggcagcc atcagtctct tctgccaaag ttctccaaca gagactctaa gataaacatc
     1681 catatcataa tcattgtgat gcgttttaaa aggggtagcc tctgctcctc cggttgtaac
     1741 ctctaaaata ggtgtttcta cctcaagaaa attttgggat cttaaaaact tcctggtaac
     1801 ctcccaaaat ttagctctct gaataaaaat ttcttttgca tctttattaa ctaaaatatc
     1861 taaatagcgc tttcttagtc ttgtctcctc atcatgaatt ccataaaaat cactgggtat
     1921 cggcaataaa gactttgata atatagtgat ggataaaacg cctaaacttt tctcgcctct
     1981 tttagtttct aaaagatatc ctcgaacttg taaaaaatct cctatatcta aattgtcgcg
     2041 ccaaaaaata aaatcatcac caatatcttc ttttttaacc accaactgga tagaggcagt
     2101 gtcatcctgc agatccataa ataatatccc tccttgatct cttatagatc gtatccggcc
     2161 aacaagataa atctttgttt tcttcgccga cagtgaacta aaactcttca gcgcatcgct
     2221 aataactctg ctgcgttttg cgctcgcagg ataaggatta attcctctat ctttaatgtt
     2281 tttcagtttt cgcaaccttt cctctcttaa tacttctatc ataaatggta ttcataatta
     2341 taaataatga cgcctcttaa atcaaaaatc gcctcaatat cataaggcga tctaattact
     2401 gtacaggaat gataaatcct atgttatctt aaaaatttta tattgtattt tcttcgcctg
     2461 cggagtctca accgttaccg agtcgccgac tttcttattt atcaattctc taccaagagg
     2521 agattcattg gatattctac cctcttctgg tcgagcttca ttgctaccaa ctataaaaaa
     2581 cttcatagtc ttcccatctc ttaatacttc aacggtggaa ccaatatcaa tgattgtttt
     2641 gtctatgttt ttcttaataa ttgtcgcatt ctgaacgatg ccttccagat cgtctattct
     2701 tctttcaacc ctttcttgtt catcttttgc ttccaaatac tcggcgtttt ctgataaatc
     2761 acccagctct ttggctcgct ttaaacgttc tgatatctca atcctcttga ccgtcttcaa
     2821 actgcttaat tcggttttta actcagctag cctgtcttct gttaagaagt aattcatttg
     2881 ttttattaag tgttaatttt ctaatattag ctaaaagact atctctttct ccattgatga
     2941 cctctcctgg ccttgctgtg tccatatttt tttctctcga ccgcccttgg atcacgcgtc
     3001 aaataaccat gagctctgaa tttctttttt aaatcaccgt ccaacttagt cattgccctt
     3061 gcaagcccta aacagattgc atctgcttga gccattattc cccctccaac cgcttttaca
     3121 gatacagcct tcaccgcatc ctctccgatt aactttaacg gcgccagcgc tttctctctg
     3181 tacctaagcg tggtgatata atttttgata tctttatcgt taataacaaa cttgcctttt
     3241 atatcctgcg gagctgaagt cggatagatc ctaaccctga ccgtggcgct ttttcttctc
     3301 cctacggctt ctatataatt tttgctgttt ttcgtttctt tagtaatcat tattttaatt
     3361 tgtttccgct ataaaactat ttttccacct ttagcatttt taacctttgt tgacgcaaag
     3421 catttttagg caacatgcca gctacagcat gttttaatac ccactccggg ctcctagaaa
     3481 aagcttcttc gtatctcctt tccttaaggt gcccgacata tccagtatgg cgatagtata
     3541 tcttctgttg agctttatta ccggtaactt ttaataaact tatattttta ataacaacag
     3601 tattgtctcc cctttttctt ggggcgtatt tagcgtctgt ttttccctgc agaatgccgg
     3661 ctatcttagt agctaatctg cctaatgtct ttccttttgc atcaattaca tattccataa
     3721 ttttatttta ctaattctaa tatcacctgc ggggcggcgt ctcttttcct tgacgcagac
     3781 attttgatta tacgagaata tcctccgcgc ctatctttat atctcggagc gatatcatag
     3841 taaagtttaa aagcggtttt cttaggcatg cggctaataa gcagccttaa tgaggctaaa
     3901 tcgttttttt tagcaagagt gattaatttt tcagctttag gcttaatctc tttggctcta
     3961 gcctcagtag tctttatttt ctcctcaata aaaaaattat gaagcaggcc gttaatgaaa
     4021 cttttccttc ttcccttgac tcttccaaat tttctgccct tatttaaatg cctcatctct
     4081 attttttgtc ctcatttttc tccgcgcctt cggcggcatc tttaatttca atgtcggata
     4141 tcctcttaaa atgatccaat aatatgtcgg ccgccttatg gaccgctcta gacggcgaaa
     4201 tagttccatc tgtttcaacc tctaaacgaa tcttgttata gtcaatcctc tctccaacac
     4261 gcatattaga taccgtaaaa ttcgcgttga tgactggcga gaagatcgca tccaaagcga
     4321 ccgttccaat aggaagcttt ccagctttct gagaaattac cggaacatat cccaatccct
     4381 tttcaaccgt tatttctata tccagttctg cgcttttatc tgtaatcgtt gcaatgtgca
     4441 cttctggagt tttaacttct acatcagcgt tgcctttaat atccgcagct gttatttctt
     4501 tctctccttt aacttttaga gtcaatacct gaggctcttt tgcgtgaaaa gcaaacctaa
     4561 cctttttcaa attcaatcca atttctaata catcctcaac aactccgggg attgttgtaa
     4621 attcgtgacc aactcctttt attttaaatc tggtaatcgc ggctccaggc agagaagaaa
     4681 gcaaaactct gcgaatagca ttacctatcg taataccata acctctatac aaaccatcga
     4741 tgtcaaaaac gcccttctta tcgtcttcag atactctatt aatttttacg ctctcgctta
     4801 aatatacaaa ttccataatt tttattttat tattttattt tttattttta tcttaagtag
     4861 taatcgacca cttggcttac atcaaatgat gaatctatgt ctaaaggagt tgataaaacc
     4921 tttccttcaa gtttttcttt atctaataaa agccatgctg gcgcttcgta attttttaat
     4981 ttttctgcga tatcttttaa ttgtcctatt tcttttgatg cgggtctaat attaattata
     5041 tctccctctt tcaccgaata agaaggaatg gtaactcttt tgccattaac aataaagtgt
     5101 ccgtgagaaa ccattttcct ggccgctatc cttgactcag agaatcctaa tctaaagata
     5161 acgttgtcta acctagattc gagcatctcc ataactatct taggcgtcgg atcggtcatt
     5221 ttctccgctc ttctaaaaat acctctaagt tgtttatctg taagaccata agtaaatctt
     5281 attttctgtt tttcctgaag ttgggttcca aattctccga ttttatgcct atttttgcca
     5341 tgcataccag gacgataagg ctttctaatc aaagcgcact taggcgagtt gcatctatgt
     5401 ccttttatag aaagtttagc tcctaagctt ctttctcttt tttctaaaat cttatgcata
     5461 ttatttatca gttaatttat atttatacgc gacgaacttt tggcggctta ggaccgttgt
     5521 gcggaacggg ggtaacatct ctaattgata aaatattaaa gccgtgatta atcaacgatc
     5581 taagagccga gtctctgccg ctgccaacgc cagaaacgaa aacatcaaca tcctgcggac
     5641 ccgtctttga tatcttttcg gctattgctg caataatttt tgatgctgca aaaggagtcg
     5701 cctttttggg gcccgaaaaa cccaaagatc ctgccgttgc ccaagccaaa acatctccct
     5761 tctcattagt agcagtaact acagtgttat tatatgaggc attaatatag attctgccat
     5821 tctgtatctt cttgccctgt accttaacaa ctttatcgcc agattcgtcg gatttagatg
     5881 cgcttgtttt taccgtaata tccggtttct tttctataat tttatttttt cccatatgta
     5941 taaataatat tatttcaatt caacttttct tcggccgcta ccagcagtct ttctaacgtt
     6001 gccgcgcacc gttctagaat tggttttcgt cctttgccct cttaccggga gttttctcat
     6061 gtgccttatt cctctatatg atttaatatc ttttaatcta gctatattct gttttattac
     6121 ttgccttagc tctccctcta ttttatattt tttctctatt acatctttga tggaattaat
     6181 ctcttctgct gtcagatctt tagctttttt gcctaaatca atctgcgccg cagtcaatat
     6241 gtttttagcc atagatctac ctacgccata aacataagta agcgatattt ctatcctttt
     6301 ttcgtctgga atatttatac ccgctagtct cataattaaa taaatttaaa gtttaaatat
     6361 caaaatgtaa aatttaggtt ctctgctgta ggcagacttc attaattttt aattctaaac
     6421 tttgcatttt tatttttgtt attatccctg cctctgttta tgtttcggat ttacacaaac
     6481 tatataaagc cgccccttgc gacggacggt cttacaatct ttgcatatct ttttaacaga
     6541 tgctcttact ttcatatcga ttgaaaaata taatttaaaa tccaggctat gtctatagcc
     6601 tgcgagttat tctccccctc ttatcatcat aggcgcccat ctcaactaaa actctgtcac
     6661 cgggtaaaat tttgatatga tgcatcctaa tcttgcccga agcataggct aaaatctccc
     6721 tattatcatc cagcttaacc ttgaaggtcg tagccggcaa tgcttccacc accataccct
     6781 gcagggtcgt agtattttta ccaggagagc tagccattaa tctatagtaa aaaatcgaga
     6841 actcagccgc ttattagata atctaaacat tatgagggtt aatatcataa cacggtatat
     6901 tattctacgt caatatcaat cttggacggc ttatcgggaa tagaaaaaac ccatattggc
     6961 caaaggctaa ccctggcttt taagcctggg atagaagaga taagctcttc tacttctttt
     7021 ttggattctc ctctaatatt ttctctaaat tgagtcagat ttaaagccct agtccaaaca
     7081 gaatcaaact gaacagacaa ggacatctct cccttaataa aatctattct tggttcgcca
     7141 tactcaatat ttctatttat taattcaata tcaatattgc ctccctccaa cacagaatta
     7201 ttatttatag attcatcccc ttcagcttcg gataaaagtt tttccctgag aaccgaaatc
     7261 aaatcagcct ctttaaagcc gactgctttt atctttgcgg ttacagaaat tttaaaattt
     7321 ccaccttcat caaccatatc gtcaacgacc tcttcaacaa tactgaattg aatagcttta
     7381 tctaaaattt ttatatcatt cggcgcatta gaaagaaatg cagatctcaa actattttca
     7441 agctctgttc ttaaattgct tttagcgttt tttatgtctt ctgatgtcgg aatcttaact
     7501 tctccgataa aaccgcccga catcggcata tccgattccg cataaaaacc ttcatatttt
     7561 ggcgaaccct gaaaaccagg gatcctaaat ttagacttag ctatattgta ttcagtccct
     7621 gtttggtcag ccatgacctg gacttcaatc gttgatggga caattttact atcaacaatt
     7681 tttgctccag ggacaacaat atcgttataa attctgaata tctttccgtc tggcgtaaca
     7741 aaccttgtat ttttaacaag tggctgggag tcagagctgt atgcattaaa aatagtcaat
     7801 ttaccctctg cttttctttc aactgtatct actcctgtag ccggatatga tttctctaga
     7861 aggtcttggt cttcaaacaa tgctccggaa accttcaatt gattcataga aaactcggat
     7921 ttagctatag aaggagaaat caaaagagat ccttcaaatt cccaattcaa tttatcaaaa
     7981 attagtgtta tatgcgcgcg gggcaaaaca aaaatagcaa cccctaagat tcctagaata
     8041 aatatagata ttcccaccaa aaacataggc ctcttaatag taatctgagg agtggtaaat
     8101 ttaggcatct ttcttttctc tttccttacc tttatcttct cttttagcca atctttttct
     8161 tcttcttctg agaatgtatc agcttcagct ttatcttcat cttcatcaag tataatcggc
     8221 gtattaggag ataagatgtc agacacgggt ttagacctct tttgaaaaaa cggattagta
     8281 gctttcatgt taagagaagc tgcaattttt aaaattttgt catcaaccga ctctatatat
     8341 attttcttgc cggcagcttc ggtttctctc ttcaaaagtt ttaagttatt aatcgtgctt
     8401 aaactttctg taaacctggg aatataaatt attaattccc tttcactaat atttaaaatc
     8461 ctttctatta tagaagatat gctgtcgtgt ttatctatat aaattttctt agccattaga
     8521 aaaaattata ctacatcagc gcgtattcta cgaacaccgg cagagctagc ttcttcctta
     8581 actatcttaa actcccctat ctcagaagtg tttttaacat gcgggccgcc acaaaattct
     8641 tttgaaaaag catcctccaa cgaattgccc gcgaaatata tatttacttc gtttggatat
     8701 ttgtcgcgaa aaaaatataa cgcgccagtt tgttttgctt catctatttt catcttcttc
     8761 atctgcatcg gaagcgcccg gcgaatcgcg ccgttcacct catcctcaac tctcttaatt
     8821 tcttcgtcaa taagtttgcg cggaaacgta aaatcaaacc gggtacgctc cgcggttatg
     8881 tccgacccca tttgtttgac ctccggcccc aaaactttgc gcaaagccgc ctgcaacaaa
     8941 tgagttgcgg tgtgaagacg ggtaactttt ttcaactctt cttcgttagc ggctttaagc
     9001 tcccctgtat tcaatatcaa cccatgcccg ccgaattttt tttcctgccc ggcgcgagat
     9061 atttcttgat gatgcgcgaa ctcacgctca aaatcggcgc gtttaagatc cttcacctta
     9121 tcgcccgcca aatcttttat cacttcgtat ggcaaaccat aagattcgta aaacttgaag
     9181 gcaagtttaa cgtcaatcga atcggactta gcaatttcac gctctccttc ttttagaatc
     9241 ttctcgaatc tatccttttc ttcgccgaaa accgacaaaa tattttgaat attcagttcg
     9301 ggatatgcgc ctgaatagtt tttgactatc gtttcaaaaa tatttttcat ttccatgcct
     9361 tgtgaagcga caataagcct tctcatcaac cggcggagaa tgtacgaagc tcccttgttt
     9421 gacgggcgga cgccatcgga tattaggaac gaaattgcgc gaacgtgatc tgctataatt
     9481 ctttttttac ttgtgtctaa ctctgccggc agtaaatcta taattaactg gaataaatca
     9541 gtttcaaaaa tgtttttcgc attctgcgct atcattacca agcgctccaa gcccataccc
     9601 gtgtctacgc ctggtgtctc gagcggcttt aaaactttcc caggtgtttc tgctaataat
     9661 tcttcgcgcg agccagggta aaaaaactgg ttaaaaacta tattccaaat ttctacatct
     9721 tttcctgaag catttttaca atatatttca gttgtcgaac cgcatggacc ttcgttcccg
     9781 gttgggcccc agaaaacatc ttcgattccc tcttcgcgca cgtcttttac accgagttcg
     9841 ttccaaatac ttttagattc tttatctttc ggcactcc
//
LOCUS       DATDNZ010000009         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1355134, whole genome shotgun sequence.
ACCESSION   DATDNZ010000009 DATDNZ010000000
VERSION     DATDNZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355134"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..529
                     /locus_tag="VI432_00305"
     CDS             230..529
                     /locus_tag="VI432_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496579.1"
                     /translation="MNSLGDMVSIPCRIKGSHTEHSINIPNLKDENDGSERSVWIKWV
                     KTKEANPPKPEPEPPKCKKCKKPMVWSVVFESDIKDTYGWVCGNEKCKNYKRPKF"
     gene            complement(785..1135)
                     /locus_tag="VI432_00310"
     CDS             complement(785..1135)
                     /locus_tag="VI432_00310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY4496580.1"
                     /translation="MTRFYKSIKNALAGFKDAFTHENNFRFMVFIGGITLSLMFYFPT
                     TITEKLIILIMIFSVLALELINSVIERIMDFIYQEHHKTIKEIKDVMAFIVLFVSLGA
                     AIVGLIIFLPYIFY"
     gene            complement(1119..1940)
                     /locus_tag="VI432_00315"
     CDS             complement(1119..1940)
                     /locus_tag="VI432_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY4496581.1"
                     /translation="MQKISGQEISESIINHLKAFKLPKKIFAVFLVGEDPISVNFIKQ
                     KEEACGRLGIDFRLYSFSESKTNDELRREIGDIVKQSSVGGAVIQLPLPRKVNKQYVL
                     NVLPKEKDLDVLGERALGAFYAGRSLVLPPAVGVIERLVEEYKINLEVSNVVVVGLGS
                     LIGKPISVWLMGKVKILNLVDSRGDIDIIRSADLIISGAGKASIIDPGILRKGASVID
                     FGYDYLDGKLRGDLDISDPSRLKHLNAYTPTPGGTGPILVAKLLENFYKLNDEIL"
     gene            complement(1901..>2471)
                     /locus_tag="VI432_00320"
     CDS             complement(1901..>2471)
                     /locus_tag="VI432_00320"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010026050.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4496582.1"
                     /translation="VTTTTHKTLRGPRGAMIFCKKELAEKIDKAVFPGMQGGPHNNVT
                     AAIAIALYEAMRPDFKKYAKQVVLNAKMLAGELKKLGIVLVGGGTESHLILLDVRNLG
                     IDGMEAQDRLEACGISANRNTVPGDPSPFKPSGIRLGTPSVTSRGMKEKDMRELASLI
                     YGALTRQKGIKIKALKLCKKFPAKKFLNQ"
BASE COUNT          788 a          558 c          436 g          689 t
ORIGIN      
        1 acgtaagaca aaaactaaaa gaagacaaaa cactgctaaa agagattaaa aacaaaatca
       61 atgcggcaat agaattagag taggcacgct ctctaatttt taatttgacc gcttaataaa
      121 tgtggtgtta tcttctcatt agaagttcct tcacagggag agtctattaa tgagtatccc
      181 cacatcgtca acagagacta aggaaagctg cctagaaaga ttaaaactaa tgaattctct
      241 aggagatatg gtttctattc cttgtagaat taagggctcg catacagagc actcgatcaa
      301 cataccgaac ctaaaagacg agaacgatgg cagcgaaaga tctgtttgga ttaaatgggt
      361 aaaaaccaag gaagctaatc caccaaaacc agagccagaa ccgccgaaat gcaagaaatg
      421 caaaaaaccg atggtatgga gtgtggtttt cgaaagcgac atcaaagata cctatggttg
      481 ggtgtgcgga aatgaaaagt gtaagaacta taaacgcccc aaattctagg aggtgatgct
      541 ctatgggttc actcaacaaa atcttcggat acatgtgcca ccgctgcata gataaaaaat
      601 gctctgagtg tacgaatcgc gaatgcggat gtaatcggag agatcacaac aagatgtgcg
      661 ctttttgcat caatggccta cataaaagtt gcaaaggaga tgggtgttct tgcgggaaac
      721 gtagtcatgc ccgcagaaag gaataatcgc cccaaaacaa actcgcgttt catacgcgag
      781 ttatttaata aaatatataa ggcaaaaata ttatgaggcc aacgatggcc gccccgagac
      841 ttacgaaaag cacgataaaa gccattacgt ctttgatctc tttaatggtt ttgtgatgtt
      901 cttgataaat gaaatccata atccgctcaa taacagaatt tatcaactct aatgctagaa
      961 ctgaaaaaat cataattaga ataattaatt tctctgtgat tgtagttgga aaataaaaca
     1021 tcaaggatag agttatcccg cctataaaaa ccataaacct aaaattattc tcatgagtaa
     1081 atgcgtcttt gaatcccgct aacgcatttt ttatgctttt ataaaatctc gtcatttaat
     1141 ttataaaaat tctctaataa ttttgcgacc aatatcggtc cagttccgcc tggagtaggc
     1201 gtatacgcat tcaaatgctt tagcctagat ggatcgctta tatccaagtc gcctcttaat
     1261 ttaccatcaa gataatcata accaaaatct ataaccgagg cgcccttccg caaaatacca
     1321 ggatcaataa tactcgcctt gcctgcgccc gagattataa gatctgccga tcttataata
     1381 tcaatgtctc ccctgctatc caccaaatta aggatcttaa ccttccccat aagccacaca
     1441 gaaatgggct tgcctattaa ggaccctaat ccaaccacga ctacattaga cacctctagg
     1501 ttaattttat actcttcgac taaacgctct attactccca ccgcaggagg caaaacaaga
     1561 cttctgccag cataaaaagc tcctaaagcc cgttcaccca aaacgtctaa atccttctcc
     1621 ttaggcaata catttaaaac gtattgttta tttactttcc gaggcaaagg aagttgtatc
     1681 accgctccgc cgactgagct ttgttttact atatctccga tctcacgcct taattcatcg
     1741 ttcgttttag attcagaaaa actatacagt ctaaaatcaa tcccaagtct gccacacgcc
     1801 tcctcttttt gtttaataaa attaactgat ataggatctt ctccaacaag aaaaaccgca
     1861 aagattttct tcggaagctt aaaggctttt agatgattta ttattgattc agaaatttct
     1921 tggccggaaa ttttttgcat aattttaaag ctttaatttt aattcctttc tgacgagtta
     1981 gtgccccata tataagactg gccagctccc tcatgtcttt ttctttcatg ccgcgcgaag
     2041 tcacggacgg agtacctaat cttatgccgg atggcttaaa cggagatgga tcgccgggaa
     2101 cagtattcct attcgcgctt atcccacatg cctcaagcct atcttgagcc tccatcccat
     2161 caatacctag gtttcttacg tcaagcaaaa taagatgact ctccgttccg ccgccgacta
     2221 gaacgatccc tagcttcttt aactctccag ctagcatctt tgcattcaat accacctgtt
     2281 ttgcatactt tttaaaatcc ggccgcatcg cttcataaag agcaatagct attgcagccg
     2341 ttacattatt atgcggcccg ccctgcatcc ccggaaaaac agctttatcg atcttctcgg
     2401 ctaattcttt cttgcaaaaa atcatagcgc cccttgggcc acgaagcgtt ttatgagttg
     2461 tggttgtaac g
//
LOCUS       DATDNZ010000010         7019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1356860, whole genome shotgun sequence.
ACCESSION   DATDNZ010000010 DATDNZ010000000
VERSION     DATDNZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356860"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VI432_00325"
     CDS             complement(<1..230)
                     /locus_tag="VI432_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496583.1"
                     /translation="MKNQLLIFILSIFFIGTASALAVWNAPTQDPPGANTDAPINTGG
                     IGQIKEGGISVGANSSTTVGLRVLNGSVGIGIG"
     gene            complement(249..527)
                     /locus_tag="VI432_00330"
     CDS             complement(249..527)
                     /locus_tag="VI432_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496584.1"
                     /translation="MELTKEQKIFIISGALLAVLIGIWIFFIIPPKNGAPVGPPTSTP
                     PPPIDIDNIINPVSPQPGSGSGNNGNIFNNTTLSGDEYQKLIKELSSD"
     gene            complement(527..1135)
                     /locus_tag="VI432_00335"
     CDS             complement(527..1135)
                     /locus_tag="VI432_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496585.1"
                     /translation="MSINRNSRKIYILIGVLFFIAFVVFIFYPKPSQVEATNHWNNMN
                     GWAWSDNIGWISLTCDNDSCSDSDYRVRLDPFTGIFTGYAWSSNIGWIQFDSGSGYPE
                     SPFYDAKMVIVPAAICSSEVEVSGWIRALSYGGGWDGWIKMAGSTGSGGHYGVCLSVD
                     GEHLDGFAWGGDVVGWVEFDENINSIIRPPTSPIHIKEIRPN"
     gene            complement(1181..1864)
                     /gene="rnc"
                     /locus_tag="VI432_00340"
     CDS             complement(1181..1864)
                     /gene="rnc"
                     /locus_tag="VI432_00340"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY4496586.1"
                     /translation="MNLESFEDRIGTSFKNKKLLEEALTHRSYLNEIAGVGKKHNERL
                     EFLGDAVLELIVTEDLFGRFPDKEEGELTVLRAALVNSKMLSLAANKIGLTESILMSR
                     GEAKESYQTRAREKISANAMEALIGAIYLDAGYGASKKFIDEFIMSFLDGAIKEAKDA
                     KSMVQEIIQSEFKLTPTYKVLEESGPAHDRTFKVGLYIGDEMQTEALGKSKQEAEIEA
                     ARAWLESRG"
     gene            complement(1868..2377)
                     /gene="nusB"
                     /locus_tag="VI432_00345"
     CDS             complement(1868..2377)
                     /gene="nusB"
                     /locus_tag="VI432_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4496587.1"
                     /translation="MATRQLGRSIVLQSLYEWDFYGNKIDLDKTIKANIEEFAPGFNE
                     PEFVQKLAEGVIAKIGELDKIIGASAPDWPVDKLPIVDRNILRIGLYELLFADRNEVP
                     PRVAINEAIELAKTYGGQNSGKFINGVLGTVYREIGEPDKKSDREDKNVDEKTGKRED
                     KKEKKKNPN"
     gene            complement(2422..2595)
                     /gene="rpmF"
                     /locus_tag="VI432_00350"
     CDS             complement(2422..2595)
                     /gene="rpmF"
                     /locus_tag="VI432_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY4496588.1"
                     /translation="MGGVPVKRHSKSKVGRRRSHLARKPVNLSVCSNCGSPVLPHRAC
                     SNCGSYKTLNVKK"
     gene            complement(2700..3350)
                     /locus_tag="VI432_00355"
     CDS             complement(2700..3350)
                     /locus_tag="VI432_00355"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY4496589.1"
                     /translation="MTKQKIKVHNRYKIQDTKYYTIGIDEVGRGALAGPIAVAAVAIP
                     YKLRLRNLKDSKRLRPSKREEWFMYAMQNQSILYSVRRIYPNKIDRINISSAANLAAH
                     RAVEDLMSRYGLRKSGCRIYLDGGLYIKNKEYQRHFAAQAKTFIKGDEKINAVKLASV
                     IAKVSRDRYMQRLHPFYPEYGFYIHKGYGTLLHREAIKRHGPSNVHRLTFIKKVIA"
     gene            3395..6598
                     /locus_tag="VI432_00360"
     CDS             3395..6598
                     /locus_tag="VI432_00360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4496590.1"
                     /translation="MKFVHLHTHSHYSLLDGLSKINPLVEKAKELGMDALAITDHGNL
                     YGAVEFYKKCKKEGIKPIIGVETYIAKDSRLSKSPRIDNIRYHLTLLAKNKSGYKNLT
                     KLITKSNLEGFYYKPRIDRELLEKYHEGIICLSGCFSGEVAKAIRTERIDDAKEIASY
                     YKGIFGDDYYLEIQPHSKDLHPGLIKLSKELNIPLVATQDSHYINLEDSPIHEVLLAI
                     QTNNRLEDEDRLTFKDFNASLRSATEMTELFSAIGGSASGGKDHLLDAIGNTVRIAEK
                     CIFEFELNKTLLPKYQLPDGESANSYLNRLIEEKSPRRYEKITPEINQRIEYELGVIE
                     KTGFADYFLIVQDFVNWAKERGIVVGPGRGSAAGSIISYILGITDVDPIKYNLLFERF
                     LNPMRISMPDIDLDFADLRRDEVLQYIKEKYGEDHVAQIITFGTMAARASVRDAGRAL
                     GFSYDFCDEIAKTIPFEPNLDKSDTKLEGYIQNVPELQHKYQTDPDARKLLDSATKLE
                     GVARHASVHACGVVVSKEPLTEYMSLQRSPQDETAIITQFEMHSVEDLGLLKIDLLGL
                     RTLTIIEETLRLLKETKNINIKVSDISIDDKSTYKFLQEGETTGVFQFESSGMRRYMK
                     DIKPTDLEDLIALVALYRPGPMDLIPSFIKRKFGEEEVEYLHPKLEPILKNTYGIGVY
                     QEQMMQIARDLAGYSLPEADTLRKAIGKKIKSLLDEQKEKLIKGMTANGIDKKTAGKI
                     WELFPPFARYGFNRSHAVCYALIGYQTAYLKNYYPVEFMTSLLNNATNDIERIAFLIN
                     EARRMGIEVLGPDINRSVGEFIPEDKNIRFGISAIKNIGENITNAIIEERFRSGPYED
                     LEAFISRIEHKDLNKKSLESLAKSGALDVFGIERMTIIDNMDDILKAKSGMRKTNENQ
                     SNLFGDSGRGIKLKLAESKPASKTQKLNWEKELLGLFVTDHPLKDFSSTEEAKKFITI
                     SQALSLPEGRNIKTYGLISKIQSFQTKKGDQMLFAKIEDLSDTIEVLVFSTVLKENPS
                     IWTEGNTIKIAGRISKKNGEPKLICNQVARLRPAIQY"
     gene            6600..>7019
                     /locus_tag="VI432_00365"
     CDS             6600..>7019
                     /locus_tag="VI432_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY4496591.1"
                     /translation="MNKNGEGLDNIRHSLAHLLAAAVLKKFPKTKLGIGPTIENGFYY
                     DFLFEKPISADLLPELEKDVEEMIKADLPFSGEKVTPLKAKKLFASQPFKLDLIKDLS
                     KEKKQLTTYRTGDVFIDLCRGGHVESTSEINPSAFKLT"
BASE COUNT         2259 a         1499 c         1286 g         1975 t
ORIGIN      
        1 cctataccaa ttcctacgga gccatttagc actcggaggc ctactgttgt tgaagagttt
       61 gctccaactg atattccacc ctctttgatc tggcctattc caccagtatt aatcggggca
      121 tctgtgtttg ctccaggggg gtcttgagta ggcgcgttcc agacggcaag ggcggaggca
      181 gtgccgataa aaaatattga taatataaat attaataatt ggtttttcat aaaagttaaa
      241 taacttgatt agtcagagga taattctttt attaattttt gatattcgtc gcctgataac
      301 gttgtgttgt taaaaatatt tccattgttc ccggagccag acccaggctg tggtgaaact
      361 ggattaataa tattatctat atctatcggt ggcggaggcg tgcttgtcgg cggacccaca
      421 ggagcgccgt ttttaggcgg aataataaaa aaaatccaaa taccgatcag gacggccaaa
      481 agagctcccg agataataaa tattttttgc tctttagtaa gttccattaa ttaggcctta
      541 tttcttttat gtggattgga gaggtcgggg ggcggataat gctatttata ttttcatcaa
      601 attcaaccca accaacaaca tctcctcccc aagcaaaacc atctagatgc tcgccatcaa
      661 ccgaaaggca aactccatag tgaccaccgc tgccagtact ccccgccatt tttatccatc
      721 catcccatcc gccaccataa gatagggcgc ggatccatcc gctaacttca acctcgctcg
      781 aacaaatcgc ggcaggaaca ataaccatct tcgcatcgta aaaaggagac tctgggtatc
      841 cacttcctga atcgaattgt atccatccta tatttgaaga ccaagcataa cccgtgaata
      901 tgccagtaaa cggatcaagc cttacacgat aatcagaatc cgaacagcta tcattatcac
      961 aagttaagct aatccaacca atattgtcag accacgccca accattcata ttattccagt
     1021 gatttgtcgc ctctacttga gatggcttag gataaaaaat aaatactacg aaagcaataa
     1081 aaaataaaac tccgatcaaa atataaattt ttctagaatt tctattaata ctcatcagtg
     1141 taaatattat acattataac ctgtatagaa ctaaaccgtc ttatcccctg ctctcgagcc
     1201 aggctctagc ggcttcaatc tccgcctctt gcttggattt acccaacgct tcagtctgca
     1261 tctcatctcc aatatataaa cccaccttga aagtcctgtc atgagctggt ccgctttctt
     1321 ccaaaacttt atatgtgggc gttaatttaa actcgctctg aataatttcc tggaccatgc
     1381 ttttagcgtc ttttgcttct tttatcgcgc catctaaaaa agacattata aattcatcaa
     1441 taaacttttt agacgctccg tatccggcat ccaaatatat cgcgccgatc aatgcctcca
     1501 tggcgtttgc cgatatcttt tccctggccc ttgtttggta cgactcttta gcttctccgc
     1561 gagacatcag aatactttcc gtaaggccga tcttattagc cgcaagacta agcatttttg
     1621 aatttaccag cgcagcgcga agaacagtaa gctctccctc ctctttatcg ggaaatcttc
     1681 caaaaagatc ttctgtcact attaactcta aaacggcatc acccagaaac tctaaccttt
     1741 cgttgtgctt cttgcccacg ccagcaatct catttaaata agatctgtga gttaacgcct
     1801 cttctaataa ttttttattt ttaaacgatg tgccaattct atcctcaaaa ctttctaaat
     1861 tcataaacta attgggattt ttcttttttt cttttttatc ctctcttttt cctgtttttt
     1921 catcaacatt tttatcctct ctatcggatt ttttatccgg ttcgcctatc tctctataaa
     1981 ccgttcctaa aacgccattt ataaatttgc ccgaattctg acctccataa gttttagcaa
     2041 gctctatcgc ttcgtttatg gcaacccttg gagggacttc attcctgtca gcaaataaaa
     2101 gttcatataa tccaatgcgt aatatatttc tatcaacgat cggcagttta tcaactggcc
     2161 aatccggagc agaggctcca attatcttat caagctcgcc tatcttagcg ataactccct
     2221 cggcgagttt ctgcacgaat tccggttcat taaatccagg agcaaattct tcgatgttag
     2281 ctttaatagt tttatccaag tctatcttat taccataaaa atcccattca tatagggatt
     2341 gtaaaacgat tgatcttcct aattgccttg tagccattag agatagtata taggaataaa
     2401 ctattaacgt gtagtattca actacttctt aacgtttaat gttttatacg agccgcagtt
     2461 agagcatgcg cgatgaggca aaaccgggct gccgcaatta gagcaaactg acaaattaac
     2521 cggctttcta gccagatggc ttcttcttct tcctactttt gattttgaat gtcttttgac
     2581 tggaactcca cccatgccga ttatttaagc ttatgaacga agcgaagtca agcttttaaa
     2641 taatcgagcg tctcgctatc ttaacggatt tcgttttata tagagagggg cttcctgatt
     2701 taggctatga ccttcttaat aaacgtcaat cggtggacat tagagggtcc atgcctcttt
     2761 atggcttcac gatgcaaaag tgtcccatat cctttatgaa tataaaaacc atattctgga
     2821 taaaacgggt gcaatctttg catataacgg tcacgtgaaa ccttagcgat gacggaggct
     2881 aatttaactg cgtttatttt ttcgtctccc tttataaaag tcttagcttg agcggcgaag
     2941 tgtctctgat attctttatt ctttatatac aatcctccat ctaaatagat cctgcatccg
     3001 ctctttcgca aaccatatct agacatcaaa tcctccacag ctctatgcgc ggcgaggttt
     3061 gcggcagatg agatgttaat tctatctatc ttattaggat aaattctccg caccgaatat
     3121 aaaatagatt gattctgcat cgcatacata aaccattctt ctcttttaga tgggcgaagc
     3181 ctcttggagt ctttcaagtt cctcaacctt aatttatatg gaatggcgac tgcggccaca
     3241 gcgattggtc cggcaagcgc cccccgtccg acctcatcaa tgccaatggt atagtatttg
     3301 gtatcttgta ttttgtatct attatgaacc ttaatttttt gtttcgtcat tttctcctat
     3361 actatatacc atatacttga taccttatac tcttatgaaa ttcgttcacc tacatacaca
     3421 ttctcactat tcccttctag acgggctctc taagataaat ccgctcgtag aaaaagctaa
     3481 agagctcgga atggatgcgc tcgcaataac cgatcatgga aatttatacg gagcagtaga
     3541 attttataaa aaatgcaaaa aggagggaat caaacccatc ataggagttg aaacctacat
     3601 agctaaggac tctcgccttt ccaagagtcc tagaatagat aacattagat atcacttgac
     3661 tcttctggcc aaaaacaaga gtgggtataa aaatctcaca aaacttataa ccaaatctaa
     3721 cctcgagggt ttttattaca aaccaagaat agatagagaa cttttagaaa agtaccacga
     3781 agggataata tgcctctccg gatgtttctc cggagaagtt gcaaaggcta tacgaacaga
     3841 aagaattgat gacgcgaaag aaatagcttc atactataaa ggaatatttg gcgatgatta
     3901 ttatctagaa atacaacctc actcaaaaga tctccatccc ggacttataa agctttcaaa
     3961 agaattgaac ataccgcttg ttgcgactca agattctcat tatatcaatc tggaagatag
     4021 tccaattcac gaagtcttgc ttgccataca gacaaacaac agattagaag acgaggacag
     4081 attaacattc aaagatttca atgcatctct acgatcggcc acggaaatga ccgagctgtt
     4141 ttccgccata ggcggatccg cctctggcgg aaaagatcat ctactcgatg ctattggtaa
     4201 tactgtgcgt atagctgaaa aatgcatttt tgagttcgag ctaaataaaa ctcttttgcc
     4261 taaatatcaa ctgcctgatg gagaatctgc taattcttat ttaaataggt taatcgagga
     4321 aaaatcaccc agaagatacg aaaagataac gccagaaata aatcaaagga ttgaatatga
     4381 acttggtgtt atagagaaaa ctggctttgc tgactatttc ttaatagtcc aggatttcgt
     4441 caactgggca aaagaaagag ggatagtcgt cggtccgggc agaggctcag ccgccggaag
     4501 tatcatctca tatattctgg ggattaccga tgttgatcct ataaaatata atcttctttt
     4561 tgagagattt ttgaatccga tgagaatttc tatgcccgat atcgaccttg attttgcaga
     4621 ccttaggcgc gacgaggtct tgcaatatat aaaggaaaag tacggggaag accatgtggc
     4681 gcagattata acctttggaa caatggccgc tagggcatcc gtaagagacg cgggaagagc
     4741 tcttggcttc tcttatgact tctgcgatga aattgcaaaa acaatcccat tcgaaccgaa
     4801 ccttgataaa tccgacacaa aattggaagg ctacattcaa aatgtacccg aattacagca
     4861 taaatatcag acagatcccg atgccagaaa attgcttgac tcagcaacta aactcgaggg
     4921 tgtagcaaga cacgcatcgg tccacgcttg cggagtagtg gtctcaaaag aacctcttac
     4981 cgaatacatg tcgctacaga ggtctccgca agacgaaaca gcaataataa cccaatttga
     5041 gatgcatagc gttgaagact taggccttct aaaaattgat cttctggggc tccgaactct
     5101 cactatcata gaagaaactc tgcgcctact aaaagaaact aaaaatataa atataaaagt
     5161 ctctgatatt tcaattgacg acaaatccac ctataaattt ctacaagaag gagagacgac
     5221 tggagtattc caattcgagt catccggcat gagaagatat atgaaagaca tcaagccaac
     5281 cgatcttgaa gatttaatag cgcttgtcgc gctatatcgt cccgggccga tggatcttat
     5341 cccttccttt attaaaagga agttcggcga agaagaagtt gaatatctcc acccaaaact
     5401 tgaaccaata ctcaaaaata cttatggaat aggagtttat caagaacaaa tgatgcaaat
     5461 agcgagagat ctcgccggct attctctgcc ggaggcggac acgcttcgaa aagctatcgg
     5521 taaaaaaata aaatcacttc ttgatgagca gaaagaaaaa ctaatcaaag gaatgacagc
     5581 aaacgggatt gataaaaaaa ctgccgggaa aatttgggaa ttatttcctc catttgcaag
     5641 atacggattt aacagatctc acgctgtttg ctacgcatta atcgggtatc aaaccgcgta
     5701 tttaaaaaat tattatccgg ttgaatttat gacctctctt ttaaataacg caacaaacga
     5761 catagaaaga atcgcatttt taataaatga ggcgaggcgt atgggaatcg aggttcttgg
     5821 accggacata aacagaagcg ttggagagtt cattccggaa gataaaaata taagatttgg
     5881 catatctgcg atcaaaaata tcggagaaaa cataaccaac gcaataatag aagagaggtt
     5941 tcgtagcggg ccttacgagg acttagaagc tttcatttcg cgaattgagc acaaagatct
     6001 aaacaaaaaa tcccttgaat ctttagctaa aagcggagca ctcgatgtct ttgggataga
     6061 gagaatgaca atcatagaca acatggatga catacttaaa gctaaaagcg gaatgagaaa
     6121 aaccaacgaa aaccaaagta atttatttgg agattcggga cgtggaataa agcttaaact
     6181 cgcagaatca aaacccgcgt ctaaaacaca aaagcttaac tgggaaaaag aattattggg
     6241 ccttttcgta acagaccatc cactaaaaga tttttcatct accgaagagg ctaaaaaatt
     6301 cataactatc agtcaggcgc tatccttacc cgagggaaga aacataaaaa catatggact
     6361 aatatccaaa atacaatcat tccaaaccaa aaaaggagat caaatgctct ttgcgaaaat
     6421 cgaagaccta agcgatacta tagaagtctt ggtattctcg accgttttaa aagaaaatcc
     6481 atcaatatgg acagagggaa acaccattaa aatagccggc agaatatcaa agaagaatgg
     6541 agaaccaaaa ttaatctgta atcaagtagc gcgactaaga cccgccatac aatattaaaa
     6601 tgaataaaaa cggagaagga ctagacaata ttcgtcattc actagcgcat ttactcgcgg
     6661 cggctgtttt aaagaaattt cccaaaacaa aacttggaat aggaccaact atagaaaacg
     6721 ggttttatta tgatttctta tttgaaaaac caatttctgc tgatcttctg cctgagttgg
     6781 aaaaagacgt ggaagaaatg ataaaagcgg atctcccgtt ctctggagaa aaagtaaccc
     6841 cactgaaagc caagaaactt tttgccagcc agcctttcaa gctagatctt ataaaagact
     6901 tgtcaaaaga gaaaaaacaa ttgactacgt atagaacagg agatgtattt atcgacttat
     6961 gtcgcggcgg acacgttgaa agcacttcag agataaatcc aagcgctttt aaattaacg
//
LOCUS       DATDNZ010000011        16651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2661326, whole genome shotgun sequence.
ACCESSION   DATDNZ010000011 DATDNZ010000000
VERSION     DATDNZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16651
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661326"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VI432_00370"
     CDS             complement(<1..744)
                     /locus_tag="VI432_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY4496592.1"
                     /translation="MKNSYKNFFWVIILLFSLSLIISGLYSEVKEPEQINISQLADKI
                     KTGGIERITVNGNDLKIYAGNDIFKSKKEPESGITDTLTRLGIAPEELSKIGIEIQDE
                     SGFNFWVGILVPTLLPLLIIVGIIWFMMRRAKTGLNQAFTFGRANIKRFVSLKDKVTF
                     KDVAGLKEPKQELVEIVDFLRSPKKYLDIGAKIPRGVLLMGSPGTGKTLLARAVAGEA
                     NVPFFHISASEFVEMFVGVGAARVRDLFET"
     gene            complement(824..1921)
                     /locus_tag="VI432_00375"
     CDS             complement(824..1921)
                     /locus_tag="VI432_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY4496593.1"
                     /translation="MQQTTKKRESMQVQILKTEPGMMPILKIGDLVEAALVERNSKGV
                     FFEIPKVGTGIVYGRELMSARDILKKLQKGDVVTAKIADSENESGFIELSLAEAGQQK
                     TWQSIKELKEKDEPIKVKVVNANSGGLITEVGGVQAFLPASQLSNEHYPAGAENDRNK
                     LIEELQKFIGEELTVKIINLNPRTNKLIVSEREVLSQDIKELIAKYNVGDIIDGIISG
                     VANFGAFIKFADNPEVEGLIHISELDHRLIENPKEVVEIGNMIKAKITEIKDNKISLS
                     LKALQADPWEKIKDIFQEGQIINGRVHKFNPFGALIKLNEDIMGLIHVSEFGSVEEMK
                     KILEESKSYKFSIDSIKPEDKRVILKLAKES"
     gene            2021..2578
                     /locus_tag="VI432_00380"
     CDS             2021..2578
                     /locus_tag="VI432_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4496594.1"
                     /translation="MIINVSEKKGIKCQIGDLAVLVDPEKKASSTVVLFTKTNWPAEW
                     TEGDIINGQGEYEASGVRIKGILLEKESSSKLMKTAYIVVMDDIKMCFLGEVSNDLDD
                     VVLDKIGEVDILFMAIGKPSIDVKKAVAIIKQIEPKMIIPTNMSHIKDLASELGQKTI
                     KEDKLVIKKKDIETVNSKLIWIAGE"
     gene            2590..5019
                     /gene="gyrA"
                     /locus_tag="VI432_00385"
     CDS             2590..5019
                     /gene="gyrA"
                     /locus_tag="VI432_00385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY4496595.1"
                     /translation="MEDSKEKINQREITTELKESYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILWAMWDSGLTHSAKLRKSANVVGEVLGRYHPHGDMAVYDSLARMAQDFSLRYPL
                     INGQGNWGSIDGDSPAAMRYTECRLSKIAEELLIDIDKETVDFMPNYDGSRNEPAVLP
                     SKIPSLLINGSDGIAVGMATKIPPHNLTETIDASVHIVDNPDATSEDLMQFVQGPDFP
                     TGGIIYNKKAIIEAYASGRGTITTRGVADVSEKSIVISEIPYQVNKSELIIKMADLVQ
                     SKKIEGIKDIRDESDKDGLRIVIDLKNDAVPQKILNQLWSYTDLQKDFHLNMLALSDG
                     LKPETLSIKDVISSFVSHRKNVVIRRAKFDLRKAEERAHILEGLHKALGVIDKIIETI
                     KKSADKEEAHKNLVKKFNLSDLQAAAILEMKLSALAALERKKIEDELKEKIKLIEELK
                     SLLKSPAKISKVVKDELLDVKKRFGDDRRTKVVVGGLKELNIEDLIPQENTIITMSND
                     GYIKRLPPNTFKSQKRGGKGIIGSGIDESDILTHFNNADTHDNILFFTDKGRVFQTKV
                     YEIPAGSRTSKGKAIQNFLEIPSDEKVKAIVTYSGSSKDLKKESGFLFMATELGVAKK
                     TNLVDFNNVRRTGIIAIKLKKGDHLKWVRLIQPKDEIILSTGSGRAIRFKESDVRSMG
                     RSASGVRGINLRKDDHLASFDVISPNEHKSNLLVIMANGFAKQTPLEEYKVQRRGGSG
                     ILTAKVTSKTGSIVSTHVIMEEKELLSVSAKGQILRTDLSSVRTTGRSAQGVRIMRLK
                     PGDKVAAAICL"
     gene            5100..6383
                     /locus_tag="VI432_00390"
     CDS             5100..6383
                     /locus_tag="VI432_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4496596.1"
                     /translation="MNISPKTILLGIIGLIVLAVVLIITGVIPGLRPPQAFKTELSMW
                     GLEDDSAAWKSVIAAYQVQNSRISINYTGFSIEEYEQRLVDALAAGTGPDIFMFHNNW
                     LLKHKNKIFAAPAEVATPITVSELFPQVVTQDFIADNQVYALPISIDTLALLYNKNIF
                     DAKQIPVATSSWEEIHRVIPILREVADGSLSKPAIALGGSEAQVRNATDILSLIMMQF
                     GAMIVNPQELRAVFSSSGFGLQGLNFYLSFSDPLNASYSWNEKFGDSITAFLNGGTAM
                     VFAYNSDIKEIRSKNPILNFGVLPFPQLKSDFPVNLPSYWGLAVSNKTRYYADSWYFI
                     SSATTDINMSRNYINISGNPPALRTLINDFLTNPNLKVFAAQALTAKNWPHPDPSGVR
                     SVFDNMIKLSLANRTQSNQFLVQAQDEINKLLQRR"
     gene            6388..6729
                     /locus_tag="VI432_00395"
     CDS             6388..6729
                     /locus_tag="VI432_00395"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496597.1"
                     /translation="MNFINIAKAAIVECGGPGQPVCDLSQFLKTITNLFSFGLEIAFI
                     MVVVSFVGGGLWMALSAGDEGRISQGRAALRGSFIGIIIIFSSWIFINTVIELFTNCT
                     NWNVFGGIICT"
     gene            6751..7095
                     /locus_tag="VI432_00400"
     CDS             6751..7095
                     /locus_tag="VI432_00400"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY4496598.1"
                     /translation="MKSLIITSLYLSIAVLSLGTVLGQTPCPAGQICNPTSFSTIQDL
                     ILGILSSLYLLAIPIVSVMVLWGAFLMVTSAGNPEKIELGKKTIKYAVIGFIFVVLAS
                     GVSSIIQDILTP"
     gene            7174..7563
                     /locus_tag="VI432_00405"
     CDS             7174..7563
                     /locus_tag="VI432_00405"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496599.1"
                     /translation="MKIIKASILVLALMMLIGSSVPGVLGQTTTVCPPGQIPNAQGVC
                     GVSTAPTTVQGLIDLVNRVFGLLFVIVITLSSVFILWAAFLYVTARGDADKQGQAKTI
                     LIYAVVGLIVAALAWGVPKVVQNFITP"
     gene            7714..7797
                     /locus_tag="VI432_00410"
     tRNA            7714..7797
                     /locus_tag="VI432_00410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7747..7749,aa:Leu,seq:tag)
     gene            8097..8858
                     /locus_tag="VI432_00415"
     CDS             8097..8858
                     /locus_tag="VI432_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY4496600.1"
                     /translation="MDLSVETLAIPSFIAGILTFLAPCTLPLVPGYLAFISGVSLGDL
                     KIEENKKLKWKIFLNGLAFVIGFSIVFIFLGALVGFGGRHLIQYRDILARVGGVFVIF
                     FGLFMLNIFKIPFLNKDFKPKTPKIFEKGRPLNSIILGSTFALGWTPCVGPILGSVLT
                     LAGASATVGSGVALLSIFSLGLAIPFLLIALLINSSTTIINSISKYLNIISFVGGAFL
                     IFLGVLIVMGQLGVWIGYFYEFFDFINYEKLLNFL"
     gene            complement(9246..10139)
                     /gene="xerA"
                     /locus_tag="VI432_00420"
     CDS             complement(9246..10139)
                     /gene="xerA"
                     /locus_tag="VI432_00420"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEY4496601.1"
                     /translation="MTDLNKLKKEYLDYLAIEKNRSPRTRANYSRYLDKFISASGVKD
                     LKDITEEKIREFRINLASKEKIKKVTQAYYVIVIRNFLKYLAKKDYKVVSPDKIELPK
                     IPERQIQIIDYKDLELLLSAPKGETLKTLRDRAILETLFSTGLRVSELCSLNKDINLE
                     RSEVTIRGKGDKLRIVFLSDRARNAIKQYLDKRSDIEEGLFITMTRDNKPIGRITSRS
                     VERIIAHYARAAGIPKKVTPHTLRHQFGTDLLINGADLRSVQELLGHSNISTTQIYTH
                     LTNKELRDVHQAFHGKRRRRD"
     gene            10224..10496
                     /gene="rpsO"
                     /locus_tag="VI432_00425"
     CDS             10224..10496
                     /gene="rpsO"
                     /locus_tag="VI432_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY4496602.1"
                     /translation="MKMLTPRQKKNVITESQTHEKDTGSAEVQVSLLSRKIDELASHL
                     KKNPKDNHSRRGLIGMVSQRRKLLSYLSDNSPKSYGNLIKRLGLKK"
     gene            10551..11087
                     /locus_tag="VI432_00430"
     CDS             10551..11087
                     /locus_tag="VI432_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY4496603.1"
                     /translation="MSIIPNKEQRIGIFIDIQNLYHSAKNLYGARVNYSELMKALIGE
                     RKLVRALAYVVKSDPMTGEESFFDALVKAGIEIRSKDIQIFPGGAKKADWDVGMAVDA
                     IRMAPLLDIVILVTGDGDFIPLVEYLKWGLGRGVEVAAFHRTSSGKLRESTDRFIDLE
                     SIPKVIFKSLKRSTGRRK"
     gene            11093..13213
                     /locus_tag="VI432_00435"
     CDS             11093..13213
                     /locus_tag="VI432_00435"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY4496604.1"
                     /translation="MDINRKKFTLEFAGRDLVIESSSIGGQANSALIGTYGGTTVLVT
                     TVMGKQDRAIDYMPLTVDYEEKFYAAGKIIGSRFIRREGRPSEDAILGGRLIDRTIRP
                     LFDKRLRRDIQIVITILSYDEENDPEFIGLLTASTALGISDIPWNGPVAGIRIAKLVG
                     SKDFVVNPLNSFIKDNMANIEFDSFVAGKKKRVNMIELSGLSAQKGDVISAYKLALQE
                     FEKLIDFQDKIIKEIGKEKSDIKFIAKNEKLEGEIKKFLSERLEEVVYSREKSRISDL
                     KNDLIDHLEKADFAELDIKLAYSIFEEEFDNFIHQEVLGKGKRPDGRKLDEVRDLYSE
                     VGLFSRIHGSSLFIRGNTQALALTTLAAPGSEQLIETMELSGKKRFMLHYNFPPFSVG
                     ETGRLGGPGRRDIGHGALAEKAIKSLIPSKEDFPYTIRVVSEILSSNGSSSMATVSAA
                     SLSLMDAGVPIKEPAAGIAMGLMADEKSLLTDNPAYKVLTDIQGPEDHYGDMDLKIAG
                     TKSGINAIQMDVKVTGVNLKILEDAFSQAEKARLHILDFTNKIIDRPREDLSSFAPRV
                     LTYKVDPDRIGEIIGPGGKVINGIIDRTGAKSIDIDDDGSVFVGASDKKSAEAALNEI
                     MAIVKDYEVGEIVEGKIIKILEFGAIVDLGAGRDGMIHVSELKNSFVKNVEEVVKLGD
                     EVKVKVIKVENGKIGLSMKRLDPK"
     gene            13270..14394
                     /locus_tag="VI432_00440"
     CDS             13270..14394
                     /locus_tag="VI432_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496605.1"
                     /translation="MNGEEKNQQEPGLADVPVSEPASAPQPPEPVLRPTPPPADFTRI
                     PNEPVPPPPPSDISVRTMQEDVSSLSKSGGLGAEPKTFRPSDLLSKEEMFKPASSGGV
                     EEPVLPPSPQKPKISHSLVVIIGSFIFLLIAGGVAYFFVLPLFNKEVAPPPPLPAPVE
                     PPPPPVEPLPTPPPPPTLQHQSLFMEGAGVSSQEVVLDELNLANLGLKITASISGDDV
                     RPGFKDLSFMTGEDEESRVLESVELWNVVAPGNQVSKLFEDDFTGFAYFDQNGIWPGY
                     VFKLKTDPESMALREQIDEAIDVEDIAGSYIVSFYLNSPGAPIKSTFSDGLKVGEDTA
                     KFVQFSLTGASFNMLQTGDYLILSTSFNGFKEALKNLGLQ"
     gene            14401..14730
                     /locus_tag="VI432_00445"
     CDS             14401..14730
                     /locus_tag="VI432_00445"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4496606.1"
                     /translation="MDLLKKRCVPCEAGTLPLTSEEIKEYLKETSDWIFQEGNKHPEL
                     VKEFRFKDFKDAMLFVNSVANLAEKEGHHPNIDIRYNKVFLTLWTHAIKGLSPNDFIL
                     AVKIDNL"
     gene            14827..16053
                     /gene="nusA"
                     /locus_tag="VI432_00450"
     CDS             14827..16053
                     /gene="nusA"
                     /locus_tag="VI432_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4496607.1"
                     /translation="MDLKSLIQTVNQIAEDKGIEPDIVLGAIEDSLAAAYKKEYLARG
                     VIVRAEMDKESGEMIFKQIKEVVDDSTVRMGTEEDKEEEVEESEDKLPLYNSDKHILI
                     ENLAEEAPKDKNAKVGDEIEFILPSQTDFGRIAAQTAKQVIIQKLREAERDSIIKEFK
                     DKEGQIVSGVVQRFERGNVFVDLGRVTSIMPYNESIPGEHYRVGERLRFFVASVAVDM
                     KTPGIILSRAHPKFVSRLFEMEVPEITDKIVEIKAIAREPGSRTKIAVYSNVEGVDPV
                     GSCVGQRGTRVMAVTNELGAEKLDIIEWSDDPEKFIANAIAPAKAKSVEVRDHHEALV
                     LVADDELSLAIGRGGQNVRLAAKLTGWKIDVRSQSRPSEEQEGGIASASKTESLSEEV
                     DDGDKPVYHKKIEDED"
     gene            16077..16562
                     /locus_tag="VI432_00455"
     CDS             16077..16562
                     /locus_tag="VI432_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY4496608.1"
                     /translation="MNIDVIVEIPKGSRNKYELDKKSGRIRLDRVLRSSVAFPADYGY
                     IENTLSDDGDPLDVLIISRFPVFPGCVVSVRPIALFNMIDSGDNDEKIVGVPEKDPYF
                     SSWEDLKDIPDALVKEIEEFYRTVKALEPNKFVEPKGWAGVKDAEAMVEKAIEQGKVN
                     K"
BASE COUNT         5071 a         2885 c         3613 g         5082 t
ORIGIN      
        1 tgtctcgaac aagtctctta ctcttgccgc tcccactcca acaaacattt caacaaattc
       61 tgaagctgaa atatggaaga atggaacgtt agcttcccct gcgacagctc ttgcaagaag
      121 ggttttaccg gttccaggag agcccattaa aagaactcct cttggaatct tggccccgat
      181 atctaaatat tttttaggag acctcaagaa atccacaatc tctacaagtt cttgttttgg
      241 ctcttttagt ccagccacgt ctttaaatgt gactttatct tttaaagaga cgaatctttt
      301 aatattggct cgaccgaagg tgaaggcttg atttaatccg gtttttgcgc gcctcatcat
      361 aaaccagatt atgcccacta ttataagaag aggcagtagg gtcggcacta aaattcccac
      421 ccagaaattg aaacctgatt cgtcttgtat ttcgattccg atttttgata attcttcagg
      481 agctattcct aatctggtta aagtatcagt tattccggat tcaggttctt ttttggattt
      541 gaaaatgtcg tttccggcat atattttcaa atcatttcca ttgacggtga ttctttcgat
      601 cccacccgtt tttatcttat cggctaattg agaaatattt atctgctcgg gttcttttac
      661 ctcgctatat aagccggata ttataagaga caaagagaaa agcaaaatta taacccaaaa
      721 gaaattttta taagaatttt tcatagatat ttttatttat tttaacacaa tatactccaa
      781 caaatttagt ggagctatcg atatgatgtg gatggggcaa gatttaactt tctttggcta
      841 atttaagaat aactcttttg tcctccggtt taatcgagtc tatagagaat ttataagact
      901 tgctttcttc tagaatcttt ttcatttctt ctaccgagcc gaattctgat acgtgtatta
      961 agcccataat atcttcatta agtttaatta aagctccgaa tgggttgaat ttgtgaaccc
     1021 ttccgtttat tatctgaccc tcttgaaaaa tatcttttat tttctcccat gggtcggctt
     1081 gcaacgcttt cagagataga gatattttat tgtcttttat ctccgttatt ttagctttta
     1141 tcatatttcc aatctcaaca acctctttag ggttttcgat caaacgatgg tcaagttcag
     1201 aaatgtggat taaaccctcc acctctggat tatcggcaaa ttttataaat gcgccgaaat
     1261 tagcaacccc ggatattata ccgtctataa tatctccaac attgtattta gctataagtt
     1321 cttttatatc ttggcttagc acctctcttt ctgagacgat aagcttattt gttctagggt
     1381 ttaaattaat tattttaaca gttagctctt caccgatgaa tttttgaagc tcttctatta
     1441 acttattcct gtcattttct gctccggctg gataatgctc gtttgataac tgtgaggccg
     1501 gcaagaaagc ctgtactccg ccgacttcgg taataaggcc gccagaattg gcgtttacaa
     1561 cttttacttt tatcggctca tccttttctt taagttcttt aatgctctgc caagtttttt
     1621 gttggcccgc ttcagcaagt gaaagttcta taaatccgct ttcattctca gagtcagcta
     1681 ttttcgccgt gactacgtct cccttctgaa gttttttaag gatgtccctt gcgctcataa
     1741 gttctctgcc gtaaacaatg cctgtgccta cttttggtat ttcaaaaaat acacccttgc
     1801 tatttctttc aactaaagcc gcctccacta aatccccgat ttttaatatg ggcatcatgc
     1861 cgggctcagt tttaagtatc tgtacctgca tggattcacg ttttttggtt gtctgttgca
     1921 tagtcaggtt agtataattg atttttattg aaaatcaact gttttagagt tgtttttccg
     1981 gttgctagtt tggtcctctc tggggtagaa tctataaact gtgattatca atgtttcaga
     2041 aaaaaagggc ataaaatgcc aaattggaga cttggcagtc cttgttgatc ctgagaagaa
     2101 ggcttccagt acagtagttt tattcacgaa aacaaattgg ccagccgaat ggactgaagg
     2161 cgacattata aatggacagg gagagtatga agcttcaggc gtgaggatta agggaatatt
     2221 gctagagaaa gagtcttcat ccaaattgat gaaaaccgca tatattgttg tgatggacga
     2281 tattaaaatg tgttttctgg gagaggtttc aaatgattta gacgatgttg ttttagacaa
     2341 gattggagag gtggacattc tttttatggc tattggtaaa ccttcgattg acgttaaaaa
     2401 agccgttgca attataaaac aaattgaacc aaagatgatc atcccgacga atatgtctca
     2461 tatcaaagat ttggcgtctg agttggggca gaagacgata aaagaggaca aattggttat
     2521 taagaaaaaa gatattgaaa cagtcaattc aaaactgatt tggatagcgg gtgaatagaa
     2581 taaatcaaca tggaagacag caaagaaaaa ataaatcaaa gagaaattac gaccgagtta
     2641 aaagaatcgt atcttgatta tgcgatgtcg gttattgtcg gacgcgcctt gccggatgtg
     2701 cgcgacgggt tgaagccggt tcatagaagg attttatggg cgatgtggga ttctggcctg
     2761 actcattccg ctaaactaag aaaatcagct aacgtggttg gtgaggtttt agggcgttat
     2821 catccgcacg gagatatggc tgtatacgat tctttagcca gaatggcgca ggatttttct
     2881 ttgagatacc ctcttatcaa tggtcaagga aactggggca gtattgatgg agattcaccc
     2941 gccgctatgc gttacacaga atgccgcctt tctaaaatag ctgaagagct tttaattgat
     3001 atagataagg agacggttga ttttatgccg aattatgatg gctcaaggaa tgagccagcg
     3061 gttttgccat ccaaaatacc aagcctttta attaatggtt ctgatggaat cgcggtcggc
     3121 atggcgacta aaattccgcc acataatcta actgaaacaa tcgatgcgtc agttcacatt
     3181 gttgataatc cggatgcaac ctctgaagat ttaatgcaat ttgttcaagg tccggatttt
     3241 ccgacaggag gaattattta taataagaaa gctataattg aggcttatgc ctccggtcgg
     3301 gggacgatta caacaagggg agttgccgat gtaagtgaaa aatccattgt tatcagtgaa
     3361 attccatatc aagttaataa atccgagttg attattaaaa tggcggacct tgttcagagt
     3421 aagaaaattg aaggcataaa agacatccgc gatgaatcgg ataaagacgg tttgagaata
     3481 gttattgatt taaaaaacga tgctgtaccg caaaaaatcc tgaatcaact ctggagctat
     3541 accgatcttc aaaaagattt tcatttaaat atgcttgcgt tatctgacgg cttaaagccg
     3601 gaaacccttt ctataaagga cgttatttcc tcttttgtca gtcataggaa aaacgtagtt
     3661 atccgcagag caaaatttga cttaagaaag gcagaagaaa gagcgcatat tttagaagga
     3721 ctgcacaagg cgcttggtgt tattgataaa ataattgaaa ctattaaaaa atcagccgat
     3781 aaagaagaag ctcataaaaa tcttgttaag aagtttaatt taagcgattt acaagcagcc
     3841 gctatcttag aaatgaaact atctgctcta gccgctcttg agaggaagaa aatagaagat
     3901 gaattaaaag aaaaaataaa gttgattgaa gaattaaaat ctttgctaaa aagccccgct
     3961 aaaatttcaa aagtagttaa ggatgaacta cttgatgtta aaaaaagatt tggagatgat
     4021 aggagaacga aagtggttgt cgggggtttg aaagaattaa atatcgaaga tctgattcct
     4081 caagagaaca cgattatcac catgtctaat gatgggtata ttaagcgctt gccgccgaac
     4141 acatttaaat ctcaaaaaag aggtggtaaa ggaattattg gttctggtat agatgagagc
     4201 gatattttaa cgcatttcaa taacgcagat actcacgaca acatattgtt tttcaccgat
     4261 aaaggaagag tttttcagac taaggtttat gaaattccgg ctggcagcag gacaagcaag
     4321 ggtaaggcga tacaaaattt tttagaaata ccgtcagatg aaaaagtaaa agcaatcgta
     4381 acttattccg gttcaagcaa agacttgaaa aaagaatcgg gattcctatt catggcgact
     4441 gaattagggg tggctaagaa aactaactta gttgatttta acaatgttag gcgcacagga
     4501 ataatcgcaa tcaaattaaa aaaaggagac catttaaaat gggtaagatt gatacagccg
     4561 aaagatgaaa taattttaag caccggctcc ggaagagcta tcaggtttaa agagtctgac
     4621 gtgagaagta tgggcaggtc tgcttctggg gttcggggaa taaatttaag gaaagatgat
     4681 caccttgctt catttgatgt gatttctccc aacgaacaca aatcaaacct cctggttatt
     4741 atggctaatg gttttgcgaa gcaaactccg cttgaggagt acaaggtgca gagaaggggc
     4801 ggtagcggaa tattaaccgc gaaagttact tcaaagacgg gctcgattgt gtccacgcat
     4861 gttataatgg aagagaaaga actcttaagt gtgtccgcta agggacagat attaagaacg
     4921 gatttatcaa gcgttcgcac aacaggcaga tcggctcagg gtgtaagaat tatgcgtttg
     4981 aaaccaggag acaaagttgc ggcagcaata tgtttgtaag cggatattat aattggaatg
     5041 gataatataa atgtagtaat atcgcacggt tatatagaat ataacttttt agtagcaaga
     5101 tgaatatttc tccaaaaaca atccttcttg gcataatagg actcattgtt ctggcagtgg
     5161 tgttgattat tactggggtt atccccgggc ttagacctcc acaagcattt aaaacagagc
     5221 taagtatgtg ggggttagaa gatgattctg cggcatggaa atcggtgatt gcggcttatc
     5281 aagtccaaaa ttctaggatt agcatcaatt acacgggttt ttctatagaa gaatatgaac
     5341 aaagattggt tgatgcatta gctgcaggaa ccggtccgga tatttttatg tttcataata
     5401 actggctgtt gaaacacaaa aataaaattt tcgccgctcc ggcagaagtg gcaacgccta
     5461 ttacagtatc agaacttttc ccgcaggttg taacgcaaga tttcattgcc gataaccagg
     5521 tctatgcctt acctatttca atcgacactc tggctctctt atataacaaa aatatatttg
     5581 acgctaaaca gattccggtt gcgacttcaa gctgggaaga aattcataga gtcataccta
     5641 ttttaagaga ggtggctgat ggcagtcttt caaagcccgc cattgcgctt ggaggatcag
     5701 aggcgcaagt tagaaacgca actgatattt taagtttgat tatgatgcaa ttcggcgcga
     5761 tgatcgttaa ccctcaagaa ctaagagctg ttttttcttc ttctggtttt ggcttgcagg
     5821 gattgaattt ttatttatca ttttctgatc cgcttaacgc ttcttattct tggaatgaaa
     5881 aattcggaga ctcaataacc gctttcttaa atggcggtac ggcgatggtt ttcgcttaca
     5941 actccgatat taaagaaata agaagtaaaa atcctatatt gaattttggc gttttgccat
     6001 tcccgcaatt gaagagcgat tttcctgtca atttaccaag ttattggggt ttagctgttt
     6061 caaataaaac cagatattac gcagactcct ggtattttat aagttcagcg acaaccgata
     6121 ttaatatgtc gagaaattat ataaatatta gcggtaaccc acccgcgctt agaactttga
     6181 taaacgattt tttgacaaat ccaaacctaa aagtttttgc agctcaagcg cttaccgcta
     6241 agaattggcc gcatccggat cccagcggtg ttcgttctgt ttttgataac atgataaaat
     6301 tatcattagc caatagaacg caatcgaatc aatttctcgt tcaagctcaa gacgagataa
     6361 acaaattatt gcaaagacgc taaaataatg aattttataa atattgctaa agccgcgata
     6421 gtggaatgcg gaggtccggg tcagccagtg tgcgatctaa gtcagtttct aaagacgatc
     6481 acaaatcttt ttagttttgg tttggaaata gcttttatta tggttgttgt ctcttttgtc
     6541 ggcggcggac tgtggatggc tttatctgct ggagacgagg gacgtatttc tcaaggcaga
     6601 gcagctctcc gaggatcttt tataggcatc attattattt tcagctcatg gattttcata
     6661 aacaccgtta tcgagctttt cactaattgt actaactgga atgttttcgg cggtattatt
     6721 tgcacctaga atccattata attttaatta atgaaatcat taataattac tagcttatat
     6781 ctctccatcg ctgttttatc tttaggaacc gtcctcggac agactccgtg tccggccggc
     6841 cagatttgca atccaaccag cttttcaacg attcaagatt taattttagg gatactgtct
     6901 tcattatatt tattggcgat accaatagtt tccgtcatgg ttttatgggg cgcgttttta
     6961 atggtgacct ctgcaggcaa cccagagaaa attgaattag gcaagaagac aataaaatac
     7021 gcggttatcg gctttatttt tgtggtttta gcaagcggcg tatcttctat tatccaggat
     7081 attttgactc cttagtttga gattgtggaa agttatgata tatttttatc taaagaaaag
     7141 gtcgaggtat tttttaagat ttaattaaaa ttaatgaaaa taataaaagc ttcgatattg
     7201 gttttagcat taatgatgct tataggttct tctgtgcccg gcgttctagg ccagacgact
     7261 accgtttgtc ctcccggaca gatacctaat gcgcaaggcg tttgcggtgt ttcaaccgcg
     7321 cctaccactg ttcagggttt aatagacctt gtaaatagag tgtttggtct tctgtttgtg
     7381 atagttataa ctttgtcgag cgtctttatc ctctgggcag cctttctata tgttactgcc
     7441 aggggcgatg ccgataaaca aggccaagct aaaactatcc ttatctatgc tgtagttggt
     7501 cttatagttg ctgctttagc ttggggagtt ccgaaagtgg ttcagaattt cattacccct
     7561 tagtagctta aaggttcact ttgcttctta gcagaaaaca aattctgctg atatttattc
     7621 cgccggccga ttggtcggcg gaatttttat taactgacgt tgaaaattat atttataata
     7681 ggtagagttt agaagtttga tgctcggcag tatgctgggg tggtggaacg gcagacacac
     7741 tagccttagg agctagcgcc gcaaggcatg agggttcaaa tccctccccc agcaccaaat
     7801 gtagctttgt ctgtcttgat cgatttaccc tgagtttgtc gaacggagtc gaggggtcgt
     7861 aaatgactat tcctttcttg tcgtgagggc aggcgcgcga acgaacgcag gcgcgaagcg
     7921 cataattata gagctacgaa gatcagatat tacacatttg cttaaaggaa ttgagtttaa
     7981 ttttgttcta gtatactatt caagtattca aataaatatt tgaatatcat attcgaatca
     8041 aatattctga agaagaaaat cagaaattct aaattaaatg ttttataatt aaaaatatgg
     8101 atttatccgt agaaacatta gctatcccaa gtttcatcgc cgggatcctt acttttctcg
     8161 ctccatgtac tcttccgctt gtgccgggat atctagcgtt tataagtgga gtttctcttg
     8221 gagatttgaa aatagaagaa aacaaaaaac ttaaatggaa gatcttttta aatggccttg
     8281 cgtttgtcat aggatttagc attgttttta tttttctagg agcattggtg ggttttggag
     8341 gaagacactt gattcaatac cgcgatattt tagcgagggt tggaggagtg tttgtaattt
     8401 tttttggtct ttttatgttg aatatattta aaattccatt tttgaataaa gactttaaac
     8461 ctaaaactcc caaaatattt gagaagggca ggcctcttaa ctccattata ttgggttcta
     8521 cattcgccct gggttggacg ccgtgtgtcg gcccgatatt gggatcggtt ttgactctgg
     8581 caggagcaag cgcaaccgtt ggaagcggtg ttgccctcct ttctattttt tctttgggct
     8641 tagcaatacc atttctttta attgctttat tgataaattc ttctaccacg attatcaatt
     8701 cgatatccaa atatctgaat attatttctt ttgtgggagg tgcatttctt atattcttgg
     8761 gtgttttgat tgtgatgggg cagcttgggg tctggatagg ttatttctat gagttttttg
     8821 attttataaa ttatgagaaa ctgctcaatt tcttgtagtg gaataaccgc gttccatagg
     8881 ttgacaaatt tttgatagta tgacttaata taattaagtt agggtaggat gggttttgct
     8941 gcaaaatcca aatgagcacc ttaagaaagt gagaaaagta ctgagggacg tacaatggtc
     9001 aacaagaaca ccaccgtgat cgtgggaagc ccgacaatct ggaccgaaaa gctctcgcca
     9061 gtggcgattg ccgtgatcga ggctccagga gccaaaaagc tccgcgaaga cctcatgagc
     9121 tacgcgaaca gctgaagctc gtcggcccgg caaccattct cagccccctc taaagacaac
     9181 aggcggcttg atattcaaag acagactgcc gcccttcatt tcattcaagg agagaaaagc
     9241 taacgctaat ctctccttct tctttttcca tgaaaagctt gatggacatc tctcaattct
     9301 ttattggtta agtgtgtgta tatttgtgtg gttgaaatat tactatgtcc tagaagttcc
     9361 tgcacagacc taagatccgc cccatttatt aataagtctg ttccaaattg gtggcgtaga
     9421 gtatgcggtg tgactttttt agggattcca gctgcacgcg catagtgcgc aattattctc
     9481 tccacgcttc tcgaggtgat cctcccgatt ggtttattgt ctctggtcat ggttataaat
     9541 aaaccctctt caatgtcgga tcttttgtct agatattgct taatagcgtt tcttgctctg
     9601 tcagataaaa atactattcg taatttatct cccttgcctc tgatcgttac ttcagatctt
     9661 tccaggttta tgtctttatt taatgagcac agttcggata cgcgaagtcc ggtagagaat
     9721 agagtttcta aaattgctct gtcgcgtaag gtttttagag tttcaccttt tggcgcggac
     9781 agtagaagct ccaaatcttt ataatcaata atttgtattt gtctctcggg tattttaggc
     9841 aactctattt tatctgggga tacaacttta taatcttttt ttgccaggta ttttaagaaa
     9901 tttcttatta caattacgta ataagcttgg gtaacttttt taattttttc tttcgaggcc
     9961 agatttattc tgaattctct gattttctct tctgttatat cttttaggtc tttaacgcca
    10021 gatgcgctga taaatttatc taagtatcta gagtaattag ctctggttct tggagatctg
    10081 tttttttcaa tcgcgaggta atccagatat tctttcttta atttattcaa atcagtcata
    10141 ttcttattat acggttaaga acggattatc gcatataaaa taaccgtttt tttgacaata
    10201 agcgtcattt agcatattat gctatgaaaa tgttgactcc tcgtcagaag aaaaacgtaa
    10261 taactgagag ccaaactcat gaaaaagaca ctggctcagc tgaagttcag gtatctttgc
    10321 tttcaagaaa aatagatgag cttgcatcgc atcttaagaa aaacccgaaa gataatcatt
    10381 cgagacgcgg tttgatcggc atggtaagcc aaaggaggaa acttttaagc tatctctctg
    10441 ataactctcc aaagagttat ggtaatttga taaagaggct aggactaaag aagtagaaat
    10501 ttagtcgcct atctgaacgc taggcgataa ttattttata taatctgcat atgtctatca
    10561 ttcctaataa agagcagaga atcggaattt ttatagatat tcaaaattta tatcattcgg
    10621 ctaaaaattt atatggagcc agggttaatt acagtgagct catgaaggct cttatcggag
    10681 aaaggaaatt ggtgagagct ttagcttatg tggttaagag tgatccgatg actggcgaag
    10741 agagtttttt tgatgcttta gttaaggctg ggatagaaat aaggagcaaa gatattcaga
    10801 tttttccggg tggggcaaaa aaagccgatt gggatgtagg tatggctgtt gatgctattc
    10861 ggatggcccc tcttttagat atcgttattc ttgtaaccgg agacggagat tttattcctt
    10921 tggttgaata tttgaagtgg ggtttaggca gaggggtgga agtcgccgct ttccatcgga
    10981 cttcatcagg caaattaaga gagtcaaccg acaggtttat tgatttagag tccattccta
    11041 aggtaatatt taaatctctt aagcgttcaa ctggacgcag aaaataaaaa acatggatat
    11101 aaatagaaaa aaattcacgc ttgaatttgc gggtagagat ttagtcatcg aatcatcttc
    11161 aataggaggc caagctaact cagcattaat tggaacttat ggagggacta ctgtgcttgt
    11221 tacaactgtt atgggcaaac aagacagagc tattgattat atgccgctta ctgtggatta
    11281 tgaggaaaaa ttttatgccg caggtaaaat tatagggagc aggtttatta gaagagaagg
    11341 cagaccatct gaagacgcca tcttaggagg acgcttgata gacaggacca tcaggcccct
    11401 ttttgataaa agattaagaa gagacatcca gattgtaatc actatccttt catatgatga
    11461 agaaaacgac ccagaattta taggacttct caccgcctct actgctcttg gaatttcgga
    11521 tattccctgg aacggaccag tggctggtat ccgtatagca aaacttgtcg gatcaaaaga
    11581 ttttgttgtg aatccattga acagttttat taaagacaat atggctaata ttgaatttga
    11641 ttcttttgtc gccggcaaga agaaaagggt aaatatgatt gaattatccg gcctttcagc
    11701 tcaaaaagga gatgtcatat cagcttataa attggcccta caagagtttg aaaagcttat
    11761 tgattttcaa gataaaatta ttaaagaaat aggcaaagaa aagtcggata taaaatttat
    11821 agcaaagaat gagaagctag agggggagat taaaaaattt ctttcggaga gattggaaga
    11881 agttgtctat agccgagaaa aatctagaat aagcgatctt aagaacgatt taatagatca
    11941 cctggaaaag gctgactttg cagaattaga tataaaatta gcttacagta tttttgaaga
    12001 agagtttgat aatttcattc accaagaagt tttaggtaag ggcaaaaggc ctgatggcag
    12061 gaagttggat gaggttagag atttatattc tgaagtgggg ttattctcta ggatacacgg
    12121 atccagtcta tttataaggg ggaatactca agcattagct cttactactc ttgccgctcc
    12181 cggatcagag caattaatcg aaactatgga gttgtctggt aaaaagaggt ttatgcttca
    12241 ttataatttt cctccgtttt ctgtagggga gactgggcgt ttgggcggtc caggcagacg
    12301 cgacataggg cacggagctt tagccgaaaa agctataaaa tcattaattc cctcgaaaga
    12361 agactttcct tatacgataa gagttgtctc tgagattttg tcgtcaaatg gttcatcttc
    12421 tatggctact gtttcagccg cgagtttaag cctgatggat gcaggcgtac ctatcaaaga
    12481 acctgcggca ggaattgcta tgggtcttat ggctgacgag aaaagtcttt taacagacaa
    12541 cccagcttat aaggtgctga ccgacattca aggacccgaa gatcactatg gcgacatgga
    12601 tttaaaaata gctggaacta aaagtggaat aaatgccatt caaatggacg taaaggtgac
    12661 aggagttaac ttaaaaattc tagaggatgc tttttctcaa gcggaaaagg ccaggctaca
    12721 tattttagat ttcaccaaca agattataga tcgaccaaga gaggatttat ctagttttgc
    12781 gccgagagta cttacttata aagttgatcc agatagaata ggggaaatta taggtcctgg
    12841 cggaaaagtt attaatggaa taattgatag aaccggcgcc aaatcaattg atatagatga
    12901 cgacggatct gtttttgtag gcgccagcga taaaaaatca gccgaagcgg ctttaaatga
    12961 aattatggcg attgttaagg attacgaggt tggagaaata gtagaaggaa agattattaa
    13021 aatattggaa ttcggcgcga ttgttgattt aggcgccggc cgcgatggga tgattcacgt
    13081 ctccgaactt aaaaactctt tcgttaaaaa tgttgaggag gttgttaaac ttggcgatga
    13141 agtcaaggtt aaagttatca aagttgagaa cggcaaaatt ggcttatcca tgaaaaggtt
    13201 agaccctaaa taatagggta gatattatcc acacaaaaaa tttcttcaca ttgtggtatt
    13261 attaatgaca tgaatggtga agaaaaaaat caacaagaac cgggattggc tgatgtcccg
    13321 gtaagcgaac ccgcatcggc tccgcagcca ccagaaccag tgctaaggcc tactccgccc
    13381 ccagccgatt ttacaagaat tccaaacgag ccagttcccc ctccgcctcc atcggatatt
    13441 tcagtgcgca caatgcagga agatgtaagc tcgctttcta agtcgggtgg attaggagca
    13501 gagcctaaaa cgttcaggcc gtctgatttg ttaagcaaag aagagatgtt taaacctgca
    13561 agcagtgggg gggtagaaga gccagttctt cctccaagtc ctcaaaaacc caagatctct
    13621 catagtcttg tggtaatcat tggatctttt atctttttgc ttattgcagg aggcgttgcc
    13681 tacttctttg ttttaccatt attcaataaa gaggtcgctc ctccgccacc attacctgct
    13741 ccggtagaac ctcctccgcc tccagttgaa ccattaccaa ctcctcctcc gcccccaacg
    13801 cttcagcatc aaagtctatt tatggaaggg gcaggagtct cttctcaaga agtcgttttg
    13861 gacgagttaa atttagcaaa cttaggacta aaaataacag catcaatttc gggtgatgat
    13921 gtccgcccag gtttcaaaga tttatctttt atgaccggag aagatgagga gagtagagtt
    13981 ttagagtcag ttgagctttg gaatgttgtt gctcctggga accaagtctc aaaacttttt
    14041 gaagatgatt ttacagggtt cgcttatttt gatcaaaacg ggatatggcc aggctacgtt
    14101 ttcaaattga agacagatcc agaatcaatg gctttaaggg agcaaataga tgaggcaatt
    14161 gatgtagaag atatagcggg ttcttatata gtaagttttt atcttaattc accgggtgcg
    14221 cctattaaat caacttttag cgatggattg aaagtcggcg aagatacggc taaatttgtt
    14281 caattttctc ttaccggcgc gtcatttaac atgctgcaga ccggagacta tttgatttta
    14341 agcacgtcat tcaatggctt taaagaggcc cttaagaatc taggattaca ataagagtca
    14401 atggatcttc ttaaaaaacg ttgcgtgcct tgtgaggcag gtacgttacc tctgacgagt
    14461 gaagagatca aagaatatct taaagaaaca tctgactgga tatttcaaga aggtaataaa
    14521 caccccgagc ttgttaaaga atttagattt aaagacttta aagatgctat gcttttcgta
    14581 aatagtgtgg cgaatttagc cgaaaaagaa gggcatcatc ccaatattga tatacgatat
    14641 aataaagttt ttttgacttt atggacacac gctataaaag gactttctcc aaacgatttt
    14701 attttagccg ttaagataga caatctatga ggttgacttc tctggggatc atgggtaata
    14761 tcagagtatt aatgtccgcc ctgaagtgcg gacattttaa aataaaagat tctatttaat
    14821 aagaagatgg acttaaaatc attaattcaa acagttaatc aaatagcaga agataaaggt
    14881 atagagcctg atattgtttt gggtgcgata gaagattctc tcgccgccgc atataaaaaa
    14941 gaatatttag caagaggtgt tatagtgcgc gcagaaatgg ataaggagag tggagaaatg
    15001 atttttaaac aaattaaaga agtcgttgat gattctactg ttcgcatggg aaccgaagag
    15061 gacaaagagg aagaggttga agagtcggag gataaattac cattatataa ctcggataag
    15121 catattttaa tcgagaacct cgcagaagag gctcctaaag ataaaaatgc gaaagtcgga
    15181 gacgagatag aatttattct tccttcgcaa acagactttg gcagaattgc agctcagact
    15241 gctaaacaag tgataatcca aaagctccgc gaagccgaaa gagactctat tattaaagaa
    15301 tttaaagata aagagggcca aattgtaagc ggagtagttc agcgttttga gagaggtaat
    15361 gttttcgtag atttaggaag agtgacaagc atcatgcctt ataatgaatc tattcccggc
    15421 gagcattatc gtgttggcga gagattaaga ttttttgttg cctcggttgc ggttgatatg
    15481 aaaactccag ggataatttt atcgcgcgcc catcctaaat ttgttagccg tctttttgag
    15541 atggaggtgc ctgaaattac ggataaaata gtggaaataa aagctattgc tagagagcct
    15601 ggttcgcgca caaaaatagc agtttattca aatgttgagg gtgtcgatcc ggttggtagt
    15661 tgtgtcggtc agaggggaac cagggtaatg gctgttacaa atgaattagg agcggaaaaa
    15721 cttgatatca ttgaatggtc ggatgatcca gagaaattca tcgcgaacgc tattgctcca
    15781 gcaaaagcta agtcggttga ggtcagggat catcatgagg cgttagtgct tgtcgcagac
    15841 gacgaattaa gtcttgcaat cggcagaggc gggcagaatg taaggttagc ggcaaagctt
    15901 actggctgga agatagatgt aagatctcaa tcaagaccaa gcgaggaaca ggaaggcggc
    15961 atagcctctg ccagcaaaac ggagtcttta tctgaagagg ttgatgacgg tgataaaccg
    16021 gtctatcata agaaaataga agacgaagat tagtcgaatt aaacacaaaa attactatga
    16081 atatagatgt tattgtagaa ataccaaaag gatctagaaa taaatatgaa ttagacaaaa
    16141 agagcggacg cataagattg gatagggttt tgcgcagttc agtcgcattt cctgctgatt
    16201 atggctatat agagaacact ctttcagacg acggagatcc tttggatgtt ctaattatta
    16261 gtcgttttcc tgtttttccg ggttgtgttg tctctgtgag accgatagca ttatttaata
    16321 tgattgactc tggagataat gatgaaaaaa ttgtcggcgt tccagagaaa gatccatatt
    16381 tttcttcttg ggaagattta aaagatatcc cagatgcgct ggttaaagaa attgaagaat
    16441 tttataggac ggttaaggcc ttagagccga ataaatttgt tgagccgaaa ggttgggcag
    16501 gagtaaaaga cgcagaagct atggtggaaa aagctataga gcaaggcaag gttaataaat
    16561 aatatatatt taatatgagc caagaattaa aaggacaata tttaatcccg acagttattg
    16621 agaagtccag tcacggagaa agggcatacg a
//
LOCUS       DATDNZ010000012         7273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_310500, whole genome shotgun sequence.
ACCESSION   DATDNZ010000012 DATDNZ010000000
VERSION     DATDNZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310500"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VI432_00460"
     CDS             complement(<1..440)
                     /locus_tag="VI432_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496609.1"
                     /translation="MMLRGIDFGPVWGASGAQGFFGEGYWYHRPLGPFGLDFSGATFV
                     AKTTTLNPRPGNMPLRRNFTPKEFLPRSIIINPVKGIALNAVGLSGPGAEALFEKHLW
                     QRREDPFFISFMSVAKTPDERIEELVAFVELFKKRLEHFRGRVG"
     gene            631..930
                     /locus_tag="VI432_00465"
     CDS             631..930
                     /locus_tag="VI432_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496610.1"
                     /translation="MKFQQKYQKIKAWWKTRKGTFNLGVILIILYFKLLERVLVVGVR
                     IRRADSLILSIMEMMALFSLVYIGKNAWLYVRDLENKARLIEQSNQSNQTPEEAT"
     gene            complement(1099..1401)
                     /locus_tag="VI432_00470"
     CDS             complement(1099..1401)
                     /locus_tag="VI432_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496611.1"
                     /translation="MTVVLLLGMLLFAIFFIVYGTAHLIGGKKFANKYVMAPTKKTLK
                     KIIVWFLKKLKRWLARVMTWLGRGVARVATRYPLPTGIVSIALVALSAILLIQFFI"
     gene            1573..2679
                     /locus_tag="VI432_00475"
     CDS             1573..2679
                     /locus_tag="VI432_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY4496612.1"
                     /translation="MTTITYKDSGVDYDAMDPFKREAQTSASRTARHLTRFGFKEVGT
                     SRGESAYLIEAPFGFLAHVEEGLGTKNLVADAMYALTGKSYYDQVAECTVAMIVNDII
                     TLGALPISVAMHLAIGDSKWLDDETRRQDLIRGWEMACHKAQCSWGPGETPTLRNMVS
                     PETAILSGSAIGVIGPENKKLDGLRIKDDDAIVMIGSSGIHANGLTLARKIADKLPEG
                     YLTKLSDGRAYGDALLDPTIIYVQLMEAIMLSGIDLHYAVNITGHGWRKLMRANADFT
                     YVVTDIPEPQPIFHFIQKYGPVDDVEAYGNLNMGAGFALYLPMNNAQAVIHMAKMLGL
                     NAWITGYIKSTVGEKKVIIKPKGIEFDSETLTVR"
     gene            2774..3094
                     /locus_tag="VI432_00480"
     CDS             2774..3094
                     /locus_tag="VI432_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY4496613.1"
                     /translation="MASQQKICSSCGTTAPVEAHFCPNCGKPMISPSTSISKQILIYL
                     ISLFIAPFGLGYAWQYLKQSDKKSKKIGITAIILTIIGITLTIFIYKSFLQSYVNILN
                     SIGI"
     gene            3119..3610
                     /locus_tag="VI432_00485"
     CDS             3119..3610
                     /locus_tag="VI432_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEY4496614.1"
                     /translation="MLPHGPKDLFNHITGRKGFDYTQITDEIFIGTNMCCQYGFSKEL
                     LSKGVRADISLEENRIDTPKGVDYFLWLPTKDHEAPSKQNIEIGVKALDTLIKNKTKT
                     FIHCKNGHGRAPTLFAAYLISKGMTVKEAINFMTAKRTSIHINESQMKALENFRTELK
                     KPQ"
     gene            3662..3886
                     /locus_tag="VI432_00490"
     CDS             3662..3886
                     /locus_tag="VI432_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496615.1"
                     /translation="MTRTQMFKLVKELIWLQPAKQEYIKAHIERYMSGGLTKYEAEKM
                     FIELQRINHRDILTRQDLEKIKLKILGFFV"
     gene            complement(3945..4286)
                     /locus_tag="VI432_00495"
     CDS             complement(3945..4286)
                     /locus_tag="VI432_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496616.1"
                     /translation="MVYFDPKDIEEMTLTRIANALPAIIAKHLSAGRNRSDLKPSDIE
                     VICNHFKPPDMYSYDLAIVIWANDFPERKHNLEDRKNGIVADVRSLMPSGTRGFVWIL
                     LAPASFGEFDA"
     gene            4442..5671
                     /locus_tag="VI432_00500"
     CDS             4442..5671
                     /locus_tag="VI432_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY4496617.1"
                     /translation="MALDKSVIESEIAKASTEISIIKLVDSIIEHAYNARASDIHIDP
                     TENSLIVRLRIDGVMKNTFTLPKEIHNEIISRIKVLAGLRTDEHQAAQDGRFKFGIKE
                     DGQTRFDLRVSIIPTYYGENAVLRLLVEQGETFTLDGIGFNERDVKIIRKAANKPYGM
                     ILATGPTGSGKTTTLYTVLKELNTEDVSIVTIEDPIEYSLDGIEQIQVNSQTGLTFAN
                     GLRSILRQDPDIIMVGEIRDEEAAGIAVNAALTGHLLLSTLHTNDAATTLPRLLDMGI
                     EPFLIASTVNIAIGQRLVRKLCKACSEKRQLTKVQVESLSDIVPSEFLGENRTFFIPK
                     GCKVCGGTGYISRIGIHEVLEISDAIRNAIVAKVPAADIKKIAVEEGMTTILQDGIEK
                     AKQGITSLEEVLRVIHE"
     gene            5710..6876
                     /locus_tag="VI432_00505"
     CDS             5710..6876
                     /locus_tag="VI432_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4496618.1"
                     /translation="MEELNKKIEEKLPAERPIAETKPIPPQKPKKKISISAFSLSPRI
                     SLAEQILFAKHLSIMLKSGISEVESLRIIQEQIKSKGFKRILSTVIENIENGQFLSDS
                     LAKFKRTFGELFINIIRLGEVSGTLPENLNYLAAEIRKKYELKGKIRAALLYPLIVLF
                     ATIGVAGALILFVLPKILPIFNSLGVKIPITTKILIAVVNIVNNYYIWILLGILTIVI
                     IWIFLLRIEKVRYVYSRVMLFMPIVGGTTKDYTMTNITRTMGLLLRSGVKIIEALKTT
                     SETVTNLVYRKALSESAEGVKKGEALYKHLKDRPDIFPTTASRMIEIGERTGSLDENL
                     IYLAEFYESELSEKVKNLSNVLEPILMVIMGLLVGFVAISIITPIYEITQGLQR"
     gene            6848..>7273
                     /locus_tag="VI432_00510"
     CDS             6848..>7273
                     /locus_tag="VI432_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496619.1"
                     /translation="MKSHKASRDKGLTPQETGERRAPSSISGFSLLEIVMVIGLMAIL
                     GGLGLFIAIDQYRSYALNAERATVVSLLQRARSQALNNLGESRHGIFIDNLNYTLFEG
                     GSYATRVQGYDEIIPKSPSVTTGGIQEITFMQLNGDSSQT"
BASE COUNT         2493 a         1512 c         1424 g         1844 t
ORIGIN      
        1 aatccaacac gccctctaaa atgctccaga cgcttcttga agagctcgac aaacgccacc
       61 aattcttcaa ttctctcatc cggagtttta gcgaccgaca tgaaagaaat aaaaaacgga
      121 tcttcgcgcc tctgccagag atgtttctca aaaagggcct cggctcccgg cccgctaagg
      181 ccgactgcat tcaaggcaat tcccttcaca gggtttatga taattgatct tggcaaaaac
      241 tccttaggag taaagttgcg tcgcagaggc atattgcctg gacgcggatt caaagttgtt
      301 gttttggcaa caaatgttgc tccgctaaaa tccaagccaa aaggccccaa aggacgatga
      361 taccaatacc cctcgccgaa aaaaccctgc gcgccagatg cgccccaaac aggaccgaaa
      421 tcaattcctc ttagcatcat gttaacgacc tgccttcaaa ctattactat atataatgag
      481 tgtcaattca gaaaatattt tatcttcctt tattccttat agagaacaag gggtaattta
      541 taaatattaa aatattaaaa agaaaaactt tgtgacaata aaaggttgac aaaattggtt
      601 ttatatatta tatatctatc aggtggctct ttgaaattcc aacagaaata tcaaaagatc
      661 aaagcatggt ggaaaacaag aaaaggaacc tttaatctcg gcgtcatcct tataatcctc
      721 tatttcaagc tcctagaaag agtgcttgta gtaggagtaa ggatacgtag agcggattca
      781 ctcatcttgt ctatcatgga gatgatggcg ctttttagcc tagtctacat tggaaaaaac
      841 gcatggctat atgtacgcga cctagagaac aaggctagac tcatcgaaca atctaaccaa
      901 tccaaccaaa ccccagagga ggcaacttag cctctttttt tctttcataa tgatcgaaaa
      961 atctccaaat cgcataatcc aattatccat aaacattagt agatattgag atgttaaaat
     1021 ttatcaaatc aaacaagata taaaattcaa ttcaaataaa aaagcaaaaa agcgccgagg
     1081 cgcttttgag agaaagaact agataaagaa ctggataagg agaatcgccg agagcgcaac
     1141 gagagcgatc gaaacgattc ctgtcggcag gggatagcga gtagcaactc ttgcgactcc
     1201 gcgacccaac caagtcataa ccctcgcaag ccatcgtttt agtttcttga gaaaccaaac
     1261 gatgatcttt ttgagcgtct tcttagtcgg agccataaca tacttgttgg caaacttctt
     1321 tccgccaatc aagtgcgccg tcccgtacac gatgaaaaag atggcaaaga gcagcatccc
     1381 aaggaggaga actacggtca taaggtcgtt cggcaccttt catcaccttg ttacttatat
     1441 tataacacac ttatctttat ttgtcaatat cattagaacc cttaccacag gctgattgat
     1501 ataaacaatt tttatctata atatgcggac agatcggact ttctgatctt cgcacattta
     1561 aaaggtgact ctatgacaac gataacctac aaagactcgg gcgtagatta cgacgccatg
     1621 gatccgttta aaagggaggc gcaaacttct gcgtccagaa ccgctcgcca cttgacccgt
     1681 ttcggcttca aagaagttgg aacaagcagg ggagaaagcg cttatctgat agaagcgcca
     1741 tttggtttct tggctcatgt cgaagaggga ctcggcacaa aaaacctcgt agccgacgca
     1801 atgtatgcgc taaccggcaa atcttattat gatcaggtcg cagaatgcac tgttgctatg
     1861 atcgtaaacg acattataac gcttggcgct ctgcctatat ctgtagctat gcatcttgca
     1921 atcggcgact caaagtggct agacgacgag acaagacgac aagaccttat cagaggctgg
     1981 gagatggctt gccacaaggc acaatgttct tggggaccag gagaaactcc aacccttaga
     2041 aatatggttt cgccggaaac agcgatactg tccggctctg ctatcggagt aatcggacca
     2101 gaaaacaaaa aactggacgg actccgaata aaagatgacg acgccattgt catgataggc
     2161 agctctggca ttcacgccaa cggactgacg cttgcgagaa aaattgcaga taagcttccc
     2221 gaaggatatc ttacaaaatt aagcgatgga cgcgcatatg gagacgctct ccttgatcca
     2281 acgatcattt acgtccagct tatggaagct ataatgctct caggcatcga tctgcactac
     2341 gcagtaaata tcaccggaca cggctggaga aaattgatgc gggcaaatgc agacttcacc
     2401 tacgtcgtga cagatattcc cgaacctcaa cctatttttc acttcatcca aaaatatggg
     2461 ccagtggacg atgttgaggc gtatggcaat ctgaacatgg gagccggttt tgccctctat
     2521 ctaccaatga acaatgccca agcagtcatc catatggcaa aaatgcttgg attaaatgct
     2581 tggatcacgg gctatatcaa gtccactgtt ggagaaaaaa aggtcatcat caaaccgaaa
     2641 ggaattgaat tcgacagcga aacactaaca gttcgctaaa acccacaaag actctcatac
     2701 acgagagtct tatttttatc taatatattt aagattatga atctatatta aaattattta
     2761 taatcatata aacatggctt cgcaacaaaa aatttgttca tcatgcggga caactgcgcc
     2821 agttgaagcg catttctgcc ctaattgcgg caaacctatg attagcccct caacttcaat
     2881 ttcaaaacaa atattaatat atttaattag tttatttatt gctccgttcg gtttgggtta
     2941 tgcttggcaa tatctaaagc agtctgataa aaaatctaaa aaaataggca tcacagcgat
     3001 tatcctgaca attataggaa taacattaac aatattcata tataaatcgt ttttgcaatc
     3061 atacgttaat atactcaatt cgatcggtat ataaatattg ttttgatata ataaaataat
     3121 gcttccccac ggacccaaag atttatttaa tcacataact ggacgcaaag gatttgatta
     3181 tactcaaata accgatgaaa tctttatcgg cactaatatg tgctgtcaat atggattttc
     3241 aaaagaacta ctatctaaag gagtgcgagc ggatataagc cttgaggaaa atagaattga
     3301 caccccaaaa ggagttgatt attttctatg gctaccgacg aaagaccatg aggcgccgtc
     3361 aaaacaaaat atagaaatag gagtcaaagc gcttgatacg ctcataaaaa ataaaaccaa
     3421 aacttttatc cactgcaaaa atggacacgg acgagctcca actcttttcg ccgcatatct
     3481 gataagcaag ggcatgaccg ttaaagaagc gataaacttt atgaccgcta aacgaacatc
     3541 tatccatata aacgagtctc aaatgaaagc gttggaaaat ttcaggactg aattgaaaaa
     3601 accacaataa gaagctaaaa taaaaaataa aattatgtca ttcttcagca caacaaaaat
     3661 aatgaccaga actcaaatgt ttaagctcgt taaggaactt atatggcttc aaccggctaa
     3721 acaagaatat atcaaggcgc acatagaaag atatatgtcc ggcggactca caaaatatga
     3781 ggcggaaaaa atgttcatag agcttcaacg cataaatcat agagacatat taactagaca
     3841 agacttagaa aagataaaac tgaagatatt aggatttttt gtgtaagcag gcttttaaaa
     3901 ataattgcaa aaaaggctcc ggttttcaac ggagcttgtt cttttcatgc atcgaactca
     3961 ccgaacgaag ctggagcaag aagaatccaa acaaaacctc ttgtgccgct aggcatcaag
     4021 gaacgaacat cagcaacgat gccgttcttc cgatcttcca aattgtgttt gcgttctgga
     4081 aaatcattgg cccaaataac aatcgcaaga tcataagaat acatatccgg tggcttaaag
     4141 tgattgcaaa ttacctctat gtcacttggc ttaagatcgc ttctattacg accagctgaa
     4201 agatgcttgg ctataatcgc cggtaaagca ttcgcaatgc gagtaagcgt catctcttct
     4261 atatctttcg gatcgaaata gaccattgtg atgggcattt tgtcgcctcc tttttaaaaa
     4321 gagcttagag actccgctct tttgctactt taagaataac cttgaaataa tataatcgtc
     4381 aacataaaaa atcttcttat ttgttataaa gctgttgatt ctataaaata gaataataac
     4441 catggcactt gataaaagcg ttatcgaaag cgaaatagct aaagcatcaa ccgagatatc
     4501 gattattaaa ttagtcgatt caatcataga acacgcttat aatgcccgcg catccgatat
     4561 tcatatagac ccgactgaga acagcctgat agtccgcctc agaatagacg gtgtaatgaa
     4621 aaacacattc actctgccca aagaaatcca caacgagatt atcagtcgaa ttaaagtgct
     4681 cgcgggactt agaacagacg agcatcaagc cgctcaagac ggcagattca aattcggtat
     4741 taaagaagat ggacaaacca gattcgacct aagagtatcc ataattccta cttattatgg
     4801 tgagaacgcg gttttaagac tgcttgttga gcaaggagaa acatttaccc tagatggcat
     4861 aggatttaac gaaagggacg tcaaaatcat ccgtaaggcg gctaataagc cttatgggat
     4921 gatccttgca acgggcccga caggatcagg taaaacaact accctatata cagtcttaaa
     4981 agaactaaat accgaggacg tttcgattgt aacgatagaa gatcctatag aatattcgtt
     5041 agatgggata gagcaaatac aagttaattc gcaaaccgga cttacatttg caaatggcct
     5101 gcggtcaatc cttagacaag atccggacat aataatggtt ggtgagataa gagatgagga
     5161 ggctgcgggc atagccgtaa acgcggcgct taccggacac cttcttttat caactcttca
     5221 caccaacgat gccgcgacaa cgctgccaag acttttagat atgggcattg aaccattcct
     5281 aattgcttca acggtgaata tcgcaatcgg gcaaagatta gtgcgtaaac tatgcaaagc
     5341 ttgcagcgaa aaaagacaac tgaccaaagt gcaagtagaa agtttatctg atattgttcc
     5401 atctgaattc cttggagaaa atagaacgtt ttttattcct aagggatgca aagtctgcgg
     5461 aggaactgga tatataagca gaatcggcat ccacgaagta ttggaaatct cagacgcaat
     5521 cagaaatgca attgtcgcca aagtgccggc tgcagatatc aaaaaaattg cggttgaaga
     5581 ggggatgaca acaattttgc aagacggaat cgaaaaagca aaacaaggaa taacttcgct
     5641 tgaagaagtt ttacgcgtga tacacgaata aaccctctca tataaaacgg ctaataaata
     5701 tatattcaaa tggaagaact aaacaaaaaa atagaagaaa agttgccagc cgaaagacca
     5761 attgccgaaa cgaaacctat tccgccgcaa aaaccgaaaa agaaaataag catatccgcc
     5821 ttctctctga gcccgcgcat ttcactcgca gaacagatac tcttcgctaa acatctttca
     5881 ataatgctaa aatccggaat ttccgaagta gaaagcttgc gcatcataca agaacagata
     5941 aaaagcaaag gttttaagag aatattatca acagttatcg aaaatataga aaatggccaa
     6001 tttctttcag actctctagc taaattcaag cgcacattcg gcgagctttt cattaatatt
     6061 atcagattag gcgaggtaag cgggactcta cccgaaaact taaattacct tgcggcagaa
     6121 ataagaaaaa aatatgaact taaaggaaaa ataagagccg cccttctcta tcctcttata
     6181 gtattattcg caacaatcgg agtagcagga gctctaatcc tctttgttct tcctaaaata
     6241 ctgccaatct tcaacagctt gggggtaaaa attccaataa ccactaaaat tttaatagcg
     6301 gtcgtgaaca tagtaaataa ttattatatc tggatacttc taggaatatt aacaatcgtt
     6361 ataatttgga tattcctttt aagaatagaa aaagtaagat atgtctattc cagagtcatg
     6421 ctttttatgc cgattgtcgg cggaacaact aaagattaca caatgactaa tatcacacgc
     6481 accatggggc ttctgctccg aagcggggta aaaataattg aggctttgaa aacaacatct
     6541 gagacagtca ctaatcttgt atacagaaaa gccctttcag aatccgcaga aggagtcaaa
     6601 aagggcgagg ctctatataa acacttgaaa gacaggccgg atatcttccc aacgacagcc
     6661 tcaaggatga ttgaaatagg agagaggaca ggaagtctag atgaaaatct gatatatctg
     6721 gccgaattct atgaaagcga attaagcgaa aaagtcaaaa atctatcaaa tgttctagag
     6781 ccgatattaa tggtcatcat ggggcttctc gtgggattcg tggctatttc aatcattact
     6841 cctatctatg aaatcacaca aggcctccag agataaagga ttaaccccgc aagaaacagg
     6901 ggaacgccgc gcgccatcgt ctatctcggg atttagcctt cttgagattg ttatggttat
     6961 tggacttatg gctatccttg gtggcttagg cctattcatc gcgattgacc aatatagaag
     7021 ctatgcgcta aacgcagaga gggcgacggt tgtaagtctg cttcagagag ctagaagtca
     7081 agccctaaac aatttgggcg aatctcgaca cggaattttc atcgataatc ttaattacac
     7141 tctatttgaa ggtggaagct atgctacacg cgttcaagga tatgacgaga taatcccaaa
     7201 atctccatcg gttacaaccg gaggcattca agagattaca tttatgcaat taaatggaga
     7261 ctcgtcgcaa acc
//
LOCUS       DATDNZ010000013         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_55295, whole genome shotgun sequence.
ACCESSION   DATDNZ010000013 DATDNZ010000000
VERSION     DATDNZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55295"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VI432_00515"
     CDS             complement(<1..771)
                     /locus_tag="VI432_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEY4496620.1"
                     /translation="MPISKNNKKNAKNTVLKLGKKEELINATFSENALKMMKKRYLYT
                     RPDGSQETPVEMFRRVSKAISEVEKKYKKDSKFIKRVEEDFFSILSSKEFTPAGRTMT
                     NAGTDQSIVANCIVLPIQDTMESIFQTLKDAALLQQAGSGLGFAFDSLRPAMYTTKRT
                     RGTSSGPVAFLEIYNTAFGIIKQQGRHGANMAMMSVDHPDVLDFLSCKKVEGSIRNFN
                     ISIKVTDKFMKAVAENSKEPWICSWGGRPVKPRKVLRRA"
     gene            complement(881..2152)
                     /gene="ftsZ"
                     /locus_tag="VI432_00520"
     CDS             complement(881..2152)
                     /gene="ftsZ"
                     /locus_tag="VI432_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4496621.1"
                     /translation="MKKNRKKNKRINKVTRQIADRKKSVKRVSKGAKSGKPKTNLVRM
                     KVVGIGGGGGNMVTRMKEDFRGVEFIIINTDIQDLEKCTVRRKIHIGRILTKGMGAGM
                     NPDLGRLAAEENMAEISEALKDTDIVFLTAGFGGGTGSGALPVIADIARELGVLTVAV
                     VTKPFTFEGSTRMRIADENIFRLKDKVDSLLVIPNDRIFNIIDNETPVIKAFEKIDEI
                     LKSAVRGMTEIISSAGLVNVDFADVQSVMSGAGSAVIGVGIASGKERAVKAVNQAINS
                     PLLETSIAGARGVLFCVAGGKDLKMSEVNEAAKIITEAVDPGAKIIFGAYTDRKLKAG
                     QIKITVVAAGFNGIPMKHEDSLDLFMAKERPQSLLDKDVETIVISEEDGGDKKQQKLS
                     NKNQKQPKDNKKTKEEDDIWEIPAFLRRKKR"
BASE COUNT          605 a          540 c          349 g          727 t
ORIGIN      
        1 agcccttctt aaaacctttc ttggttttac cggtctaccg ccccatgaac aaatccaagg
       61 ctctttagaa ttttcagcaa cagctttcat aaacttatcc gttactttaa ttgagatgtt
      121 aaaattccta atgctcccct caaccttctt acaagaaaga aaatctaata catctgggtg
      181 gtcaacactc atcatagcca tattcgcgcc atgccttcct tgctgtttaa taattccgaa
      241 agcggtatta taaatctcta aaaacgccac cggaccagaa gaagtgcctc gcgttctctt
      301 cgttgtatac atcgcaggac gcaaggaatc gaaagcaaat cccagaccgg aaccagcctg
      361 ttgcaaaaga gcggcatcct ttaatgtttg aaaaatgctt tccatcgtat cctgtatggg
      421 taaaacgata cagttggcga cgatagactg gtctgtgccg gcattagtca tcgttctacc
      481 ggcaggagta aattctttac tggataaaat gctaaaaaaa tcttcttcaa cgcgcttaat
      541 aaatttacta tcttttttat atttcttttc aacctccgag atggctttag aaacgcgcct
      601 aaacatttca accggagtct cctgactgcc gtcgggtcga gtatagagat agcgcttttt
      661 catcatcttt aaagcgttct ctgaaaaagt ggcattaatc agctcttctt tcttgccaag
      721 tttcagaaca gtattcttgg cgttcttttt attgttcttt gagataggca tgtgacaatc
      781 ctaattttaa ttccctgtcg tctcaattac aagtcaacaa aaaataaaat tttgtggata
      841 acattgcctt ttcggcaaaa aaatgatgaa acttttaaaa ctacctcttc ttgcgtcgca
      901 aaaaagcagg aatctcccaa atatcatcct cctctttagt ttttttatta tctttgggtt
      961 gtttctggtt cttattagac aatttctgct gttttttatc acctccatcc tcctcgctta
     1021 tcactatggt ctctacatct ttatctaata atgattgggg acgctcttta gccataaata
     1081 ggtctaaaga gtcttcgtgt ttcataggaa taccgttaaa tccagccgcg acaacagtta
     1141 tcttaatttg tccagctttc agctttctat ccgtgtatgc gccaaatatg atcttcgcgc
     1201 caggatctac cgcttcagtg ataattttag ccgcttcatt tacttcagac atttttaagt
     1261 ctttaccgcc ggcaacacaa aacaaaaccc ccctagcccc ggcgattgac gtctcaagaa
     1321 gaggcgaatt aatagcttga tttacagcct taaccgctct ttccttgccg gaggctatac
     1381 caacgccgat cacggccgat ccggcgccgg acataaccga ctgtacgtct gcgaaatcaa
     1441 cattaacaag acctgcagaa gaaattatct cagtcattcc acgaacggcg cttttaagta
     1501 tctcatcaat tttttcaaaa gcttttatta caggcgtttc attatcaata atattgaaaa
     1561 tcctatcgtt aggtattacg agcaacgaat caactttatc ttttaatctg aaaatgtttt
     1621 cgtctgcaat acgcatcctt gtgctgccct caaaagtaaa aggtttggtt acgaccgcta
     1681 cagttagaac tcccaactct ctagctatat cagcaataac aggaagggcg ccagaaccgg
     1741 ttcctccgcc aaacccagca gttaaaaaaa ctatgtccgt gtctttaagc gcttctgata
     1801 tttcagccat attttcttcc gcagccaagc gcccaagatc cggattcatg ccggcgccca
     1861 ttcctttagt taaaatccta ccgatatgta tttttcttct aaccgtacat ttttccaaat
     1921 cttgaatatc agtatttata ataataaact caactcccct gaaatcttct ttcattcgcg
     1981 tcaccatatt tccccctcca ccgcctatac ccacaacttt cattctaacc aaatttgttt
     2041 ttggcttgcc acttttagcg cccttactga ctctttttac agacttcttc ctgtcggcta
     2101 tctgccttgt aaccttattt attcgtttat tcttttttct attttttttc atatttttat
     2161 taaatactta cggcagaaaa ttctttaaaa acctggtaat tgcttttgac ccctttaggc
     2221 t
//
LOCUS       DATDNZ010000014         2973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2401939, whole genome shotgun sequence.
ACCESSION   DATDNZ010000014 DATDNZ010000000
VERSION     DATDNZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401939"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VI432_00525"
     CDS             complement(<1..616)
                     /locus_tag="VI432_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4496622.1"
                     /translation="MTKIYHKNIKDKKLEILNSFRIGSWIYVENPIEEELDSLSKEFK
                     LDRSLLKDAIDVYEVPRMETEGKNIYIFSRFPYSEGGQTLTAPILLVMGEDFLMTVSL
                     KKLPFLDRFTEEKIDFYTTQKTKLFLQLFSHINSSYNSFLNEINKKTRGLSIKADNIR
                     NRDIIQFVSFEEIINDFLSSLVPTNNLLQNLLSGRSLKLYEDDKD"
     gene            737..1096
                     /locus_tag="VI432_00530"
     CDS             737..1096
                     /locus_tag="VI432_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496623.1"
                     /translation="MLLVLHIIGGVLIGLLIILSFLCILFGKNRYYIWLAGAIGIGGG
                     VQLVTGSLLTMISSGSLLMFCARISLYLVTIIVAEGVLWIAMSKDKLIFPPKLVFAPI
                     IGSLAFAVFGGVITFYS"
     gene            1098..1457
                     /locus_tag="VI432_00535"
     CDS             1098..1457
                     /locus_tag="VI432_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4496624.1"
                     /translation="MDYPKTVKEIIEQNNVPAVWANEQGIIEGVNRIFTENYGWSDTE
                     LIGKSIAEIMPPQFRELHHIGFSRFLTTEQSKILGKPLPLAILFKNGTAQNAEHFILG
                     EKEDNKWRFAATIIIRK"
     gene            1462..2025
                     /locus_tag="VI432_00540"
     CDS             1462..2025
                     /locus_tag="VI432_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4496625.1"
                     /translation="MDKNTLTTKLQSGSEEILFNLRKAIAQMDLILSSLVEGVVVLNK
                     KAEIIFANDAFGRIVNTNRLFVVGKYIGEILPIAKISQDGLMSAETLSRINGVHEIIQ
                     NDRKIIIETVTAYLQNINEGVIVIRDITDNSIKETAIKERADELARMNKFMVGREIKM
                     VELKEEITKLQKTVDSLQQEMERFKKS"
     gene            complement(2137..2207)
                     /locus_tag="VI432_00545"
     tRNA            complement(2137..2207)
                     /locus_tag="VI432_00545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2173..2175),aa:Pro,seq:cgg)
     gene            complement(2310..2723)
                     /locus_tag="VI432_00550"
     CDS             complement(2310..2723)
                     /locus_tag="VI432_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4496626.1"
                     /translation="MVDIIFQKGKFWRRLANFWTFIMLAFIVFNFINGNSYNYLVVPM
                     SILYSGVLAIYVTTKEFERWYEEHKSQHPGELFVLLWTVVMFFVLAGAFILGEGYHFP
                     SDIIVGVYLGVLTLFAITQKSKEFHRRSREEENKL"
     gene            complement(2725..>2973)
                     /locus_tag="VI432_00555"
     CDS             complement(2725..>2973)
                     /locus_tag="VI432_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496627.1"
                     /translation="NEIMADLKIEPGPRLGRILTILLEDALDDPSKNLKKKLLSRAEE
                     LNKLSDAELDKIAEKAKKSAEEAQERVDKEIKDKYFVK"
BASE COUNT          991 a          584 c          514 g          884 t
ORIGIN      
        1 agtctttatc gtcttcatac aatttcaacg acctaccgga taaaagattt tgaagaagat
       61 tgttggtggg cacgagagac gataaaaaat cattgattat ctcttcaaat gaaacgaact
      121 gaataatgtc cctgtttctt atattatccg ccttgataga caggcccctc gtcttcttat
      181 ttatttcatt tagaaaagaa ttgtaagaag aattaatatg actgaataac tgaagaaaca
      241 acttagtttt ctgggtagtg taaaaatcaa ttttttcttc agtaaaacga tcaagaaaag
      301 gaagcttctt caatgaaaca gtcatcaaaa aatcttctcc cattacaaga agaatcggcg
      361 cagtcaaggt ttgtccaccc tcgctatatg gaaatcttga aaagatgtaa atattttttc
      421 cttctgtctc catacgcggg acttcataga catctatcgc atctttcaat aaacttctat
      481 caagtttaaa ttccttagac aaagagtcca attcttcttc tataggattc tccacataga
      541 tccaagagcc gatacgaaac gaatttagaa tttccagctt cttatcttta atgtttttat
      601 gataaatctt cgtcataata aataactaat tgcccgcata ttcatctcgc aaaatatatt
      661 tatataaact gattttaaca tagatattcc ttatgacgat taagcgcaga gtgttataat
      721 atctaggata ataaacatgc ttcttgtctt gcatataata ggaggagtct taatcggcct
      781 tcttatcata ctaagctttt tatgcatatt atttggcaaa aacagatatt atatctggct
      841 tgccggagcg ataggtattg gtggaggagt gcaactcgta actggatcgc tattaactat
      901 gatctcaagc ggatcgcttt tgatgttttg cgcaaggata agtctatact tggtaacaat
      961 tatcgtagcc gaaggagtcc tgtggattgc gatgagtaaa gataaattga tctttcctcc
     1021 aaagcttgta tttgctccga ttataggaag ccttgctttt gcggtatttg gcggcgtgat
     1081 aacattctat tcataaaatg gactatccta aaacagtcaa agaaatcatc gagcaaaata
     1141 atgttcctgc cgtctgggcc aacgaacaag gcatcataga gggagtcaat cgcatcttca
     1201 ccgaaaatta tggatggtct gatacagaac taatcggaaa atctatcgct gagatcatgc
     1261 cgccgcaatt tagagaactg caccatatag gattttctag attcctcact accgaacaat
     1321 caaaaatcct tgggaaacct ttgccgcttg ctatactttt caaaaacggc acagctcaaa
     1381 atgcggaaca cttcatcttg ggagagaaag aagataataa atggcgattc gcggcgacta
     1441 tcatcatacg caaatagaat tatggataaa aacacgctaa ccaccaaact acaatccggg
     1501 tctgaagaga ttttatttaa tttgagaaag gcgatagccc aaatggacct gattctctcg
     1561 tctctcgtag agggggttgt ggttttaaat aagaaggctg aaataatttt tgccaatgac
     1621 gcattcggca gaatcgttaa cactaatcgc ttatttgtag tcggaaaata tattggagaa
     1681 attctaccta tcgctaaaat atctcaagac ggacttatgt ctgcagaaac gctatccaga
     1741 ataaacggcg tgcatgaaat aatccaaaat gacagaaaaa ttataattga aacagtaaca
     1801 gcatatcttc aaaatatcaa cgagggggtc atagtaataa gagatataac cgataattct
     1861 attaaagaga cagccatcaa agaaagggca gacgaattgg ctcgaatgaa taaatttatg
     1921 gttggaagag aaataaaaat ggtggaatta aaagaagaga taacaaaact gcaaaagaca
     1981 gtagactctc tacaacaaga aatggagaga tttaaaaaaa gctaattttt atagagctta
     2041 tcaagagttc ctgattgtcg gcaaggaatc cacagtctct acaaatctat ataatagatt
     2101 tcctgtgagt acggattatt cccgttatat gttttgtcgg gctgctggga attgaaccca
     2161 gccctcacgc acccgaagcg tgtatactac cgatatacta cagcccgaca aaacatataa
     2221 ctccaaaaca tacatactta atttactaac ccccacaaaa cgatgatcgc ttagcccgct
     2281 tgaccaatat taaatctggg cttctaaaat tacaatttat tttcttcctc gcgacttctc
     2341 ctatgaaatt cttttgattt ctgggttata gcaaagagtg ttaaaacgcc taaatacaca
     2401 cctactataa tatctgacgg aaaatgataa ccttcgccca atataaaggc tcctgccaaa
     2461 acaaaaaaca taacaacggt ccaaagaaga acgaagagct ctccgggatg ctggctttta
     2521 tgctcttcat accatctctc aaattctttt gtcgtaacgt aaattgcaag aactccggaa
     2581 tataaaatac tcataggtac gactaaataa ttatacgaat tgccgtttat aaagttgaag
     2641 acgataaagg ccagcataat aaacgtccaa aaattcgcca atcgcctcca gaattttcct
     2701 ttttgaaaaa ttatatctac cattctattt aacaaagtac ttgtctttta tctctttgtc
     2761 taccctctct tgagcttcct ctgcgctttt tttggctttt tcggcaattt tatcaagctc
     2821 tgcatcgcta agcttattca attcttccgc ccttgataaa agcttcttct taagattttt
     2881 tgacggatca tcaagcgcgt cctctaaaag aatcgttaag atcctaccga gtctcggacc
     2941 cggttcaatc tttaaatcag ccatgatttc att
//
LOCUS       DATDNZ010000015         5461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2403166, whole genome shotgun sequence.
ACCESSION   DATDNZ010000015 DATDNZ010000000
VERSION     DATDNZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403166"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VI432_00560"
     CDS             complement(<1..458)
                     /locus_tag="VI432_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496628.1"
                     /translation="MKKYFILFTTIALLVLAGISTTSAAEVVYSLSADETGTALPQGV
                     TIVSADGSRVIQFDGGGYIKVGNQSNLNFGVSEPFSIAVIFKWNGKDGVNNLVRKSDY
                     PVRGDGAGYWFRIGNNKLEFFVGETVGTGAPRGSVSATISPNVWYNALGVR"
     gene            complement(505..747)
                     /locus_tag="VI432_00565"
     CDS             complement(505..747)
                     /locus_tag="VI432_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496629.1"
                     /translation="MKIMGINKLLVYVGVIIVLALSGFFIWQLTISPEEEPVPASTGL
                     GTELYEEAQNPLNEKLPETNPFDANANPFEKKYNPF"
     gene            complement(1067..1654)
                     /locus_tag="VI432_00570"
     CDS             complement(1067..1654)
                     /locus_tag="VI432_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496630.1"
                     /translation="MASSWIALAWGGGVTLVASIFTKNHWRENVYAEEYLRQGFWVSA
                     RAGVFEEIIFRWIFFYALIPWITLVNWLFLGFAGLDVLKWLFEHVLGPVANFATLGKL
                     APYLAGGASWAVGSALLSANGMFRNGHKYLGLFGFINSWFIGMFLFYIMFRYGLPVAI
                     AVHFLYDLLIFVIRYLDAVIERARGHSHREIYALR"
     gene            complement(2008..2922)
                     /locus_tag="VI432_00575"
     CDS             complement(2008..2922)
                     /locus_tag="VI432_00575"
                     /inference="COORDINATES: protein motif:HMM:NF001135.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEY4496631.1"
                     /translation="MKSGSQVILYYKYVTIVDPAQVVRSQRELCERLGLNGRILISKE
                     GINGTLEGTNKNIEKYVEAMNSDELFSGIHFKYSEGTSASFPGLSIKAREEIVTSGFG
                     AEEKVENRITGKYLKPEELKKWIESGKEFYIVDMRNDYEQKSGYFEGSILSNVPNFRD
                     IPKFIPKISHLKEKQILTVCTGGVRCEKASGYLMQNGFQDVYQLEGGIHSYMEKYPNE
                     DFKGKLYVFDKRILVGFNTEDPKHEVVSSCERCGVKSENYVNCSYDICHRHYIVCDGC
                     YQKSAGFCNLKCRFANGRVFRMMSRLVG"
     gene            complement(3017..3994)
                     /locus_tag="VI432_00580"
     CDS             complement(3017..3994)
                     /locus_tag="VI432_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496632.1"
                     /translation="MGVETPNIFPDESLIEPDKEAETESERVGSFRKTINKLFGRKAE
                     LELGNSENKQEASPKRFSLSVEMMPKLFREFKVEGKENIEAIPEGKRIILATTHTSDF
                     DIPLVAAALSRDMKLVVTHISYQNELIKSLKASDVTLGSMLVAGRKNFLPIRYVRDKN
                     QSHGRKGQINPEDVENIVSALDEGKAAIIAAHNPVFDDKLPDKPGFAAIRVAQLAGQD
                     AAVVPVAVQIGNPSEEGRTKPGRVIINRPEAKVIIGKPLTFDDPDAKAAGDRIDSILR
                     NKEEGSVLSQEENAQMKAAREVINETEGVKLMGALASLLPEEMRGNWKE"
     gene            complement(4107..4496)
                     /locus_tag="VI432_00585"
     CDS             complement(4107..4496)
                     /locus_tag="VI432_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY4496633.1"
                     /translation="MRQISIRLKPLDDLKEEIVKLAEVKNIKAGILLSAVGSLSRASL
                     RMAGGGEIKDRSADFEIVSATGTISKNGCHIHVIVSDKDGNCLGGHLNFGSIVRTTVE
                     LVILIFDDTEYKRLPDEETGYKELVVG"
     gene            complement(4498..4929)
                     /locus_tag="VI432_00590"
     CDS             complement(4498..4929)
                     /locus_tag="VI432_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HEY4496634.1"
                     /translation="MKMKQDFNRKISIVVRNDLPNWQVLNTVAHISAYFGNKLGENFN
                     PMGADFTTKDGIGYPRNTQYAIIVLSAEPNEMRDFMKSVREARMDNMAFFREMIETTD
                     DEEIQKIVGGKEDKDVEYLGIGIFGENDKVKELTKRFSLWK"
     gene            complement(4986..>5461)
                     /locus_tag="VI432_00595"
     CDS             complement(4986..>5461)
                     /locus_tag="VI432_00595"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY4496635.1"
                     /translation="DGNTLEENAMKKARFARNSSDSDIWVMADDTGLFISALNGEPGV
                     QAAYWGGEEKTTEERMMYALERLKGIEDRSATFKTIVAMISPEAQEYIFEGICEGTLL
                     ENPRVAPQPKMPYSSLFVPDGHERAWAEMTVEEENSISHRGKAFRQVKEFLSKQI"
BASE COUNT         1564 a         1345 c          891 g         1661 t
ORIGIN      
        1 cgaaccccta atgcgttata ccaaacattc ggagatattg tagccgaaac gctcccgcga
       61 ggcgcgccgg ttccaacggt ttctccaacg aaaaattcta atttattatt gccgatgcga
      121 aaccaatagc ccgctccatc accccttacc ggataatcag attttctaac taaattatta
      181 actccgtctt tgccgttcca cttaaaaatt accgctatag aaaacggctc ggaaactccg
      241 aaatttaaat tgctctgatt ccccactttg atatatcctc caccatcaaa ttgaatgacc
      301 ctgctcccgt ctgctgaaac aatggtaacg ccctggggca acgccgtccc agtctcatcg
      361 gcgctcaaac tataaacaac ttctgcggcg ctcgtagtcg aaatccccgc taaaaccaat
      421 aaagcaattg tcgtaaataa tataaaatat tttttcatct gtagttaaat tatttgttat
      481 taatttatta aataagtcta gttgttaaaa tgggttgtat ttcttttcaa aaggattcgc
      541 gtttgcgtca aacggatttg tttcgggcaa tttttcattc aatggatttt gcgcttcttc
      601 atacaactcc gtacccaagc cggttgacgc aggaacaggc tcttcttcgg gcgatatggt
      661 caattgccat ataaaaaatc cgcttagggc cagaacaata atcaccccta catataccaa
      721 aagcttattt atacccataa tcttcattac cttgtacttt attatatagg atttcttata
      781 aaactcagaa tctgcaaatc atggatcaat ctaaatgtat aaatctttaa ctcattactc
      841 tgcccttcga ctcataattt agtgataact cgaagatttt aaacagaatg attattacac
      901 cacactgaat ataaatctaa aaatccgtcc atatcaaaaa tagcggcctt gatagtatta
      961 ttctctaaaa attcgcgatt gattttgatc atctagtcta caataaaacg gctttaccaa
     1021 aataaaaagg aggccgttga ttcaaacggc cccacccatt gcatatctac cggagcgcat
     1081 agatctctcg gtgactgtgt ccccttgctc tttcgattac cgcgtccaaa tagcggatca
     1141 cgaaaatcag aagatcgtat aggaagtgaa ctgctatcgc gacaggaagt ccatagcgga
     1201 acataatgta aaagagaaac atcccgatga accatgaatt gatgaaccca aacagaccga
     1261 gatacttatg gccgttacgg aacattccat ttgcagaaag tagcgcggag cctacagccc
     1321 aactcgcgcc tccagcaaga tacggcgcga gtttaccgag cgtggcaaag ttcgcaacag
     1381 gtccgagaac gtgctcgaac aaccacttaa ggacgtctag tccggcgaat ccaagaaaca
     1441 gccaattcac aagagtgatc cacgggataa gggcgtagaa aaaaatccat cggaagatga
     1501 tttcttcaaa cactcccgcg cgcgcgctga cccaaaatcc ttggcgtagg tattcctctg
     1561 catacacatt ctccctccaa tggttctttg taaaaataga tgccaccagg gttacgcccc
     1621 cgccccacgc taacgcgatc caggatgatg ccaatatcgc gcgtagatca cctctcacca
     1681 cccaaaattg gaaaagagaa aagggaatta tttttggcga gaaaagctcg agaagaacaa
     1741 cgaataaaaa aacgatggcc gagagcgtga ttagagccct tatacctatt tgtttctcgt
     1801 ttgcgcttat gtgaattcct cctagttttc aaagatccgt cacagacaga atgcttgaca
     1861 taataaaaca aataagccga taagtcaagg ttaaaaaaga aaaagagccg acgagcaaaa
     1921 tgcttcgtcg gctttaaata gtgtccaaaa tctacagctt gacttggatg tcaaataaat
     1981 atggcattgg ctggcctaac gaatctccta cccgaccaat ctgctcatca ttctgaaaac
     2041 gcgcccgtta gcgaagcggc attttaggtt acaaaaaccg gcggattttt gataacagcc
     2101 atcgcagaca atgtagtgtc tatgacaaat atcatatgag caattgacat aattttcaga
     2161 tttaactccg cacctctcac agcttgaaac aacctcatgt ttcgggtctt cagtattaaa
     2221 tccgactaaa atcctcttat caaaaacgta taacttgcct ttaaaatcct cgttcggata
     2281 tttttccata taagaatgta tcccgccctc aagctgataa acatcttgaa atccattttg
     2341 catcaaatat cccgatgcct tctcacaacg cactcctccg gtgcatactg ttaaaatttg
     2401 tttttctttt agatggctga ttttaggaat aaattttgga atatccctaa aattcggcac
     2461 attagataaa atcgatcctt caaaatatcc actcttctgc tcataatcat ttcgcatgtc
     2521 aactatataa aattcttttc cgctctctat ccatttcttt agttcctcgg gttttaaata
     2581 ttttccggtt atacgatttt caaccttctc ttcggcgcca aatccgcttg ttactatctc
     2641 ttctcttgct tttatgctta gccccgggaa agacgcgctc gttccttcac tgtatttaaa
     2701 atggattccg gaaaaaagct catcggaatt catcgcttcg acatattttt caatattctt
     2761 atttgttccc tcaagagtgc cattaattcc ctctttggaa atcaaaatcc tgccatttaa
     2821 tcccaagcgc tcacaaagct cgcgctgact acgaacaacc tgggcgggat caacgatagt
     2881 tacatattta taatagagta tgacttgaga accgcttttc atataagacg gagtttacac
     2941 aaatattatc aaatctcaaa gtgactctat tacaccacaa cggacatcaa atatagcagt
     3001 cttaattata tagagcttat tctttccaat tgcccctcat ttcctccggc aacaaagacg
     3061 ccaaggctcc catcaattta actccttccg tttcattaat tacctctctt gctgctttca
     3121 tctgcgcgtt ttcttcctgc gacaaaacgc ttccttcttc tttatttctc aaaattgaat
     3181 cgattctatc acccgctgct ttagcatcag gatcgtcgaa agttaacggc ttgcctatga
     3241 taacttttgc ttctggccgg tttataataa ctcttccagg ctttgttctt ccctcttctg
     3301 aagggttgcc tatttgaaca gcaaccggaa cgactgccgc atcttgaccc gcaagttgcg
     3361 caacccttat agcagcaaat ccaggcttat caggaagctt gtcatcaaaa accgggttat
     3421 gggcggcgat tatagccgcc tttccctcgt ccagagcaga aacaatattc tctacatctt
     3481 cgggattgat ctgtcctttc ctcccatgac tttgattttt gtctcttaca tatcgaatgg
     3541 gtaaaaaatt cttccgtcca gccacaagca tgcttccaag cgttacatca gatgccttaa
     3601 gagattttat aagttcattc tgataggaaa tatgagtaac aacaagtttc atgtcgcggg
     3661 acagggcagc ggcaacaagc ggtatatcga agtccgaagt atgcgtggtt gcaagtatta
     3721 ttcttttacc ctctggaatc gcttctatgt tttctttacc ttccacctta aattctctaa
     3781 ataacttggg catcatttct acagagagcg aaaacctctt cggtgaggct tcttgtttat
     3841 tctctgaatt tcccaattct aattcggctt ttcgcccgaa taacttattt atagtcttac
     3901 ggaaactgcc gactctttca gattcggtct cagcctcttt atcaggttca attaaagact
     3961 cgtcaggaaa aatatttggc gtttctactc ccataaccta actataaatt aattatacta
     4021 gttatcaaaa taatcgtttc cggctttata ggattctata aggctcaggg tttacacaaa
     4081 aattatcaaa tctcaaagtg actctattac ccaacgacca attctttata gcccgtttct
     4141 tcatcaggca atcttttata ttcggtgtca tcaaaaatta aaatcaccag ctcaactgta
     4201 gttcttacga ttgatccaaa atttagatgt ccgcctaaac aattgccatc tttatcagaa
     4261 acaattacat gaatatggca tccgttttta gagattgtgc ccgtagcgga tacaatctca
     4321 aaatccgcac tcctatcttt aatttcgcct ccgcccgcca tcctcaaact cgcacgcgag
     4381 agactgccga ctgccgagag caatataccc gccttaatat ttttaacttc agcaagctta
     4441 acaatctcct cttttaaatc atctaaaggt tttaacctaa tgcttatctg tctcatttta
     4501 tttccacaaa ctaaatctct ttgttaattc tttaacttta tcattctccc cgaaaattcc
     4561 tattcctaga tattctacat ctttatcctc ttttcctccc actattttct gaatttcttc
     4621 atcatccgtt gtttcgatca tttctcgaaa aaatgccata ttatccattc gcgcctcacg
     4681 aaccgatttc ataaaatcgc gcatctcgtt tggttcggcc gacagaacaa ttatcgcgta
     4741 ctgggtattt cgtggatagc ctataccatc ttttgttgta aaatcagctc ccatcggatt
     4801 aaaattctcg ccaagtttat taccaaaata tgctgagata tgcgccacgg tatttaacac
     4861 ctgccagttc ggcaaatcat tcctcaccac tattgatatt tttctattaa aatcttgttt
     4921 cattttcatc ttcctcttca caacattcca acattctgca gaatgttgga atgttgtgat
     4981 tgttattaaa tttgctttga taaaaattct ttcacttgtc ggaatgcctt acctctatgt
     5041 gaaattgaat tttcttcttc caccgtcatc tccgcccaag cacgctcatg tccatcagga
     5101 acaaacagag aactatatgg cattttgggc tggggagcga cacgcggatt ctcgagcaat
     5161 gtcccttcgc aaatcccttc aaaaatatat tcttgcgcct cgggagagat cattgcaaca
     5221 atagtcttaa acgttgcaga cctgtcctct attcccttta atctttcaag cgcatacatc
     5281 atcctctctt ctgttgtctt ttcttctccg ccccaatacg ccgcctgaac tcccggctcg
     5341 ccgtttaacg cagaaataaa caaaccggtg tcatccgcca ttacccaaat atcgctatct
     5401 gaactattgc gcgcgaagcg cgcttttttc atagcgtttt cttcaagcgt attcccgtct
     5461 t
//
LOCUS       DATDNZ010000016         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3205002, whole genome shotgun sequence.
ACCESSION   DATDNZ010000016 DATDNZ010000000
VERSION     DATDNZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205002"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VI432_00600"
     CDS             <1..1430
                     /locus_tag="VI432_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY4496636.1"
                     /translation="RLFDETQLMTLSNEELTSIYHFPNTNLNTPRVRIAKSASSEPPA
                     DLPKEGLVIGTNVYRGDERKVRLSREDRRRHLYIIGQTGTGKSTLIKNMIVQDIRNGE
                     GVGILDPHGDIAEDVSALVPQNRINDVVVVDPSDLSRPIALNMLEFNPNFPEQKTFIV
                     NEIINILDKLYDLKATGGPMFEQYTRNALLLLMDYPERGFTLLEIPRVLSDKTFRTEL
                     LSGCKNILVKDFWEKEADKAGGEAALANMVPYITSKFNVFLANDYIRPIISQSKSTIN
                     FREILDSKKILIVNLTKGRLGDINASLLGLIIVGKILMAAFSRVDTVEDKRSDFYLYM
                     DEFQNFATDSIATILSEARKYRLNLIIAHQFIEQLKKEIKDAVFGNVGSVLALRVGTE
                     DAKILVNQFDPVFNENDLVNIDNFNAYVKMLISGRTSKPFSIRVDKTEKGDPILAAEI
                     KKTSSSKYGRDRDEVEADIFRRIKR"
     gene            complement(1605..2222)
                     /locus_tag="VI432_00605"
     CDS             complement(1605..2222)
                     /locus_tag="VI432_00605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4496637.1"
                     /translation="MRLYIVRHGKSEGNLRGVHQTPEEKLSEAGLAQARLIAQRFSDI
                     TIDKIISSSHKRAEETAIAISEIVKQPVILSNLFTEIKRPSSIIGKGENETAEVRALI
                     RSMWHDPNWRHSDEETFFELRDRGIKGLQYILSLNSDNVLLVSHGILIDILLSLMIYG
                     NNLEPKQFEPILKFFHMENTGLTLFEYNDGHWRIVTWNDQAHLGG"
     gene            complement(2275..2345)
                     /locus_tag="VI432_00610"
     tRNA            complement(2275..2345)
                     /locus_tag="VI432_00610"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2311..2313),aa:Ile,seq:tat)
     gene            complement(2389..>3456)
                     /locus_tag="VI432_00615"
     CDS             complement(2389..>3456)
                     /locus_tag="VI432_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4496638.1"
                     /translation="PAIIFIDEIDAIGRERGTGLGGGHDEREQTLNQILVEMDGFDRD
                     TKLIVMAATNRADVLDSALLRPGRFDRRIFLDLPDISERESILKLHAENKPIDKNVDF
                     RKIASRTPGFSGADLSNLINEAAILTAQQNKKIIDQQSVYDSIEKVLLGPARKSRIVS
                     QQEKEIIAYHEAGHAIIAAGLKHSDPVHKVSIVSRGQAGGYTLKLPTEDKRLKTKSQF
                     MADLAVMMGGYITEKIVFKDITTGASSDLKEASGLARALVTKYGMSDKLGPITFGKSN
                     EMIFLGREMTADKNYSESVAITIDEEVKKLIDSALQTASKIVSTNKILLKKIADALIE
                     KEVLEQDEFYAIIKGAKLKRV"
BASE COUNT          999 a          666 c          694 g         1097 t
ORIGIN      
        1 accgactttt tgatgaaacg cagcttatga ctttatcaaa tgaagagcta acaagcattt
       61 accattttcc caataccaac ctcaatactc cccgtgtgag aattgcgaag tcagcaagtt
      121 cagagccccc agcagacctt cccaaagaag ggctggttat cggcaccaat gtttatagag
      181 gagatgagcg caaggtccgt ttaagtcggg aagacagaag aaggcattta tatataattg
      241 gtcagactgg aacgggcaag agcactttga taaaaaatat gattgtgcaa gatatcagaa
      301 acggtgaggg ggttggcata ttagatcctc atggcgacat agcggaagat gtttctgctc
      361 tcgtcccgca aaatagaatt aatgatgttg ttgttgttga tccgtctgat ctttcgcgtc
      421 cgatagcctt aaatatgctg gaatttaatc ctaattttcc agaacaaaaa actttcattg
      481 taaatgaaat aataaatatt cttgataaac tatacgattt gaaggcgact ggcggaccga
      541 tgtttgaaca atacaccagg aacgctcttc tacttttaat ggattatccc gagcgcggat
      601 ttactctgct tgagattcca agagtcttat ctgacaaaac ttttcgaaca gagcttttgt
      661 ctggttgtaa aaacatttta gttaaagatt tctgggagaa agaagcggac aaggcgggag
      721 gagaagcggc cttggcgaat atggttccct atatcacatc taaattcaat gtctttttgg
      781 ccaatgatta tatcagacct atcatttcac aatctaagag cactattaat ttcagagaaa
      841 tattagactc taaaaagata ttgatagtaa atttgaccaa gggaaggctg ggcgatataa
      901 acgcttctct tttgggcctt ataatagtcg gcaagattct gatggccgct ttttctaggg
      961 ttgatacagt ggaggataag cgttcagact tttatctgta tatggacgaa tttcagaatt
     1021 ttgcgactga ttcaattgcg acaattttat cagaagcgcg aaagtatcga ttgaatctta
     1081 ttattgctca tcaatttatt gagcaattaa aaaaagaaat taaagatgct gtgtttggaa
     1141 atgtggggag tgttttagct cttagagtgg gcacagaaga cgccaaaatc ctagttaatc
     1201 aatttgaccc agtttttaat gaaaacgatt tggttaatat agacaacttc aatgcttatg
     1261 taaaaatgct catctcgggc agaacctcta aacccttttc tatccgcgtg gataaaaccg
     1321 agaaaggaga cccgatttta gcagctgaga ttaaaaaaac atcttcatct aaatacggac
     1381 gcgatagaga tgaagtagag gccgatatct ttagaagaat taaaagataa taaataagag
     1441 tttctctcgt ataaaaaata aagaagtcgg ttaattttcc gaaaagtcag attttggtgc
     1501 tcttcccagg tctcggaatt tatagaacga gaccccatga gggattctca ggttatttca
     1561 gtataacaga cttaggcgcc tcggtagagg catataagat tagatcaccc gcctaaatgc
     1621 gcttgatcat tccaagttac aattctccag tgcccatcat tatactcaaa tagagtaagg
     1681 ccagtatttt ccatatggaa aaattttaga attggctcaa attgtttagg ttctagattg
     1741 ttgccgtata tcatgagaga taaaagtata tcaatgagaa tgccgtggct aaccagaaga
     1801 acattatcgc tattcaaact aagtatatat tgaaggcctt ttattccgcg gtctctaagt
     1861 tcgaaaaagg tctcttcgtc ggaatgtcgc cagtttgggt catgccacat cgatcggatc
     1921 agcgccctaa cttcagctgt ttcattttct ccttttccta tgatactact agggcgtttt
     1981 atttcagtaa agagattaga taaaataacc ggctgcttca caatctcgct tattgcgatc
     2041 gcagtttctt cagctcgttt atgagaacta gagattatct tatctatcgt aatatctgaa
     2101 aacctttgtg ctattaatct agcttgagct aagccagcct cagataattt ctcctcggga
     2161 gtttgatgaa cgccgcgcag gttaccctca cttttgccat gccgaacaat atatagtctc
     2221 atcggttaat ttattataat gcctatctca gactccgaat cttgaacgac ggtgtgcgcg
     2281 cacttggaat cgaaccaagt actagcgtgt ataagacgct ggttttgcca ttaaactatg
     2341 cgcgcgatat tagataagaa atagcaaata aattaaagta aagcaatgct atactctttt
     2401 taatttagcc cccttaataa tagcgtaaaa ttcatcttgc tcaagaactt ctttttcgat
     2461 cagagcgtca gctatcttct taagtaaaat cttattagtc gagacgattt ttgaggcggt
     2521 ttgaagagct gaatcgatta attttttgac ctcttcgtct attgtgattg caaccgattc
     2581 ggaataattt ttatcggctg tcatctcacg acccagaaat atcatttcgt tgcttttgcc
     2641 gaaggttatc ggacctaatt tatcgctcat gccgtatttt gtgacaagag ctctcgctaa
     2701 gcctgaagcc tcttttagat cgcttgatgc gccggttgta atatctttga agactatttt
     2761 ttccgttata tatccgccca tcataacggc taaatcagcc ataaattgag actttgtttt
     2821 taatctttta tcttcggttg ggagttttag agtatatccg cccgcttgtc cgcgggagac
     2881 aatagagact ttatgcactg gatctgaatg ctttagtcct gcggcgataa tggcgtggcc
     2941 cgcttcgtga taagctatta tttctttttc ttgttgtgaa acaattcttg attttcttgc
     3001 tggtccaagt aaaacttttt caattgaatc ataaacactt tgttggtcta tgattttttt
     3061 attttgttgc gcggttaata ttgccgcttc atttatcaag tttgaaaggt ctgctcctga
     3121 aaaacctgga gttcttgatg ctatttttct aaaatcaaca tttttatcaa ttggtttgtt
     3181 ctcagcgtgc aattttagaa tagactctcg ttcgcttata tccggcaggt ctaagaagat
     3241 tcttctatca aatcggcctg gacgcagcaa tgctgaatcg aggacgtcag ctcggttggt
     3301 cgcggccatg acaataagtt ttgtgtctct atcgaaacca tccatctcta caagtatttg
     3361 attaagggtt tgctctctct catcatgtcc tccacctaag cccgttcccc gttcgcggcc
     3421 tattgcatca atttcatcaa tgaagattat ggcagg
//
LOCUS       DATDNZ010000017         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3731182, whole genome shotgun sequence.
ACCESSION   DATDNZ010000017 DATDNZ010000000
VERSION     DATDNZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731182"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..448)
                     /locus_tag="VI432_00620"
     CDS             complement(89..448)
                     /locus_tag="VI432_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY4496639.1"
                     /translation="MTKRSETGALGEDLATKNLLKKGYKIIERNYREKWGEIDIICIN
                     PENVLVFVEVKTVNKGIFTPEDQMTKSKLNKVARTAEIYSKMFYVKHRCDEIRIDLIG
                     ITIEADKAKLKHYQGIA"
     gene            complement(816..1505)
                     /gene="uppS"
                     /locus_tag="VI432_00625"
     CDS             complement(816..1505)
                     /gene="uppS"
                     /locus_tag="VI432_00625"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY4496640.1"
                     /translation="MNIPKHIAIIPDGNRRWAKKKNLSPSQGHQRGAETAELITREAI
                     KLGIPHITAWGSSYDNLTKRPIYEIKILNKLYKEFADRLAKDKDIIKHEVKVTFLGEW
                     KDLLEPYVIDAFKNTEYKTKKYNKHFLTCLIGYNGDREMIAAIKSLRKSDDPVTNDSI
                     KKHLWTSALPSVDLLIRTGNEPHLSTGFMMWNIKYAELYFSNKLWPDFTVADLKKAIK
                     YYATRERRYGK"
     gene            complement(1579..1653)
                     /locus_tag="VI432_00630"
     tRNA            complement(1579..1653)
                     /locus_tag="VI432_00630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1619..1621),aa:Val,seq:tac)
     gene            2019..2405
                     /locus_tag="VI432_00635"
     CDS             2019..2405
                     /locus_tag="VI432_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496641.1"
                     /translation="MKRVDFTSEQEEIIKNIGEQVDSRYKSDIERGIETEKRQIFKEV
                     IEDHSKTIDSDDLKREQAEHVKEFNSTPEDNRLQKLVDYAASKGPIEASILVKKIGDP
                     WLEDQLHDVLIKFHDELIKRGRLKEE"
     gene            2513..>2870
                     /locus_tag="VI432_00640"
     CDS             2513..>2870
                     /locus_tag="VI432_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496642.1"
                     /translation="MYYLILSISAVIALALVLIFLNILFKKKIFFWRITRSLNMKLIS
                     ITLPVSRLDDAQKEKEAISLMEQFYSSLSSIRSYKFGYFAPKPYIILELAVSHIGEEI
                     SFYLSAPEKIMDSIEKQ"
BASE COUNT          876 a          481 c          495 g         1018 t
ORIGIN      
        1 tcccatcttt aatttaattg gtaaacaact aggctatata tttattatat cataatatat
       61 agatattgtc aaacggcaaa agcagtattt aagctattcc ttggtaatgt tttaattttg
      121 ctttatccgc ttcgatagta attcctatta aatcaattct tatttcatcg catcgatgtt
      181 tgacgtaaaa catcttgcta taaatttcag cggttctggc gactttattc aatttgcttt
      241 tggtcatttg atcctcagga gtaaaaattc ctttattcac ggtttttacc tcaacaaaaa
      301 caaggacatt ttctggattt atacatataa tatctatttc tccccatttc tcgcgataat
      361 ttctctcaat gattttatat ccttttttaa gaagattttt agtcgctaaa tcttcgccca
      421 atgcgccagt ctctgatctt tttgtcattg ttttacgtga aacattagat tagacatata
      481 aacatcttat caacaatttg ttttacgtga aacactagat aaaaacgcat agatattagc
      541 ttttttatat tttaattgtt ttacgtgaaa cattccccta aagatatcca cagatattaa
      601 aagactgatc ttagatttat tggtctaaat gtttcacgtg aaacatttat tctattattt
      661 acttgatgat tttgttttac gtgaaacatt gataaaacac gaataacccg ataagtagat
      721 aaataaagca tttttcaagt gtaaaacatt tttgatgttt tacactttga caagtcgata
      781 aagctgttag taactgttta acagtgtttt acagtttatt ttccgtatcg tctctcacgt
      841 gtcgcataat attttatggc ttttttaaga tctgcaacgg tgaagtctgg ccatagttta
      901 ttgctaaaat ataattcagc atattttata ttccacatca taaaaccagt gcttagatgg
      961 ggttcgttgc ctgttcttat aagcaaatca acagagggaa gcgcgctggt ccagagatgt
     1021 tttttgatag agtcgttggt tacgggatca tcagattttc taagagattt aattgcggct
     1081 atcatctctc tgtctccgtt atatcctatc aagcaagtca gaaaatgttt attatatttt
     1141 ttagttttat actcggtgtt tttgaaagcg tctataacat aaggctctaa aagatctttc
     1201 cattctccca aaaaagttac tttaacttcg tgtttaataa tatctttatc tttagccaac
     1261 ctatcagcaa actccttata tagtttatta agtattttaa tttcatatat tggtcttttg
     1321 gtgagattat cataagaact accccaagca gttatgtgag gaatgccaag ttttatcgcc
     1381 tcacgtgtta ttaactctgc tgtttccgct cccctttgat gtccttggga gggtgacaga
     1441 ttcttttttt tagcccaacg cctattgcca tctggaataa ttgctatgtg tttagggata
     1501 ttcattttta acaaatttgg aaagcgattt ttaagttata gagaccactt aatttataag
     1561 ttttattcgt ctttatcgtg gtgcgcgctg aggggatcga acccccaacc ctctcggtgt
     1621 aaacgagatg ctctaccatt gagctaagcg cgcctattaa tttataataa tgcagaattg
     1681 tagattgtaa attgtaaatt gacaacccgt ttagataact ctatgctcta aacaagtaag
     1741 gttctttaag ttgaatcaaa atgatagttc cgagacagag gacttctctc tcacccgatt
     1801 ccctccggtt agaagaagtt tctctgtctc ggtagattgt tcattattat gatgctattc
     1861 ggatcaaggc gtcctcctag ccagttcgca cgcaggaccg tctccgctag aagattacat
     1921 ctttgatccg aatagcatct ctcgattatt agatccctta gaaggatcct tttttgtata
     1981 ctataattat aaggaaaccc aattaaataa tttattagat gaaaagagta gattttactt
     2041 ctgaacaaga agaaataatt aaaaatatcg gcgagcaagt cgattctaga tataagtctg
     2101 atatcgagag gggaattgaa acagaaaagc gccaaatttt taaagaggtt attgaagatc
     2161 attctaaaac aattgactcg gatgatttaa agagagaaca agcagaacat gttaaagaat
     2221 ttaatagtac gccagaagat aatcggcttc aaaaattagt agattatgcc gcttcaaaag
     2281 gcccaattga agcgtctatt ttggttaaaa aaataggaga tccttggctt gaagatcagc
     2341 ttcacgatgt tttgattaag tttcacgacg agcttataaa gagaggcaga cttaaagaag
     2401 aatgaagcgt ttttaataaa cgccctgtta aatatcaaat gaccactaat caattaaaag
     2461 agtcgatgca ggttaatgtg ggtcggctag attattttgt tatactttta agatgtatta
     2521 tctaattctt tctatatcgg cggtgattgc gctggctctc gttttgattt ttcttaatat
     2581 tcttttcaag aaaaaaatat ttttctggag gataacgcgg tctttaaata tgaagctgat
     2641 ctcaattact ttaccggtta gcagactgga tgatgcgcaa aaagagaaag aggccatttc
     2701 tttgatggaa cagttttata gctctttgtc gtctattagg tcgtataaat ttggatattt
     2761 cgcgcctaaa ccgtatataa ttttagaact tgcggtttct catattggcg aagagatatc
     2821 tttctatctg tccgctccgg aaaaaataat ggatagcatt gagaagcaga
//
LOCUS       DATDNZ010000018        20316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3211467, whole genome shotgun sequence.
ACCESSION   DATDNZ010000018 DATDNZ010000000
VERSION     DATDNZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20316
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3211467"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..622
                     /locus_tag="VI432_00645"
     CDS             89..622
                     /locus_tag="VI432_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496643.1"
                     /translation="MNNYTNRNSEHSPVYQGKHKFYISNQSGLTLIETLIYSILVAGL
                     LAGALISVYQLLEGSEELNLITTTEEEANFILRKIQWALTGIDSINFPGPGDSDSILI
                     TNKINYAQNPILFQLDGTDIKMSRGGGSLNSLNSSRVTIENLLFTHLASAGEKPAGIK
                     TEIVINGAEYETLIYFK"
     gene            623..1009
                     /locus_tag="VI432_00650"
     CDS             623..1009
                     /locus_tag="VI432_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496644.1"
                     /translation="MQKGYIALTSTIVISILILTIILAVSFTGFSTRSNLLNYYYKEI
                     TRSVAEGCINTALSKSAQDPLYEGNETIDIGQDTCMIYPIENDIPNIQKVIKAKAVLK
                     RVATNLKVTVNLLTLDIIAWEEIENL"
     gene            1040..1561
                     /locus_tag="VI432_00655"
     CDS             1040..1561
                     /locus_tag="VI432_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4496645.1"
                     /translation="MKGRDFISNLIEMKNQKGFTLIEVLIVIGIIAVLAGIVIVAINP
                     ARQFAQGNNTTRRSHVNSILNAIGQRIADNRGLFETGCAAGAIPANATLMAEDADNDP
                     LTVEYDIANCLVPTYLPSMPFDPIGDQTDGTADWVDSDDYNTGYTVLQDADGRITVAA
                     PSAQLNEVISVTR"
     gene            complement(1614..2471)
                     /locus_tag="VI432_00660"
     CDS             complement(1614..2471)
                     /locus_tag="VI432_00660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4496646.1"
                     /translation="MHYIFFGSPRFAEIVLDKLITNGFIPTALVCNPDRPVGRKKIIT
                     PPATKQLVERLITIDKQSINVEILQPETIDQSFLNRLRQLKADFFIVAAYAKILPKEL
                     LDIPFRGVIGVHPSLLPKYRGSSPIQSVLLSGKEETGVSLYLMDEKMDHGPVLKTDNL
                     EIKNQNYLELEKDLAWLGGDMLVEMLPRFIRGEITPIPQNESEATYTHKFNTEDALID
                     LGDLKIAESGDVDKTVKILGMIKAFNPEPGAYIILDGKRIKLLDAINEDGRLKLLKIQ
                     EEGKKPRAI"
     gene            complement(2572..3039)
                     /gene="def"
                     /locus_tag="VI432_00665"
     CDS             complement(2572..3039)
                     /gene="def"
                     /locus_tag="VI432_00665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4496647.1"
                     /translation="MTVKIVTIKDKKEERFLRKKTADFDFDRFSKKEVHDLIIKMKNA
                     MDEAEGIGLAANQIGLDMNVFVARVDGKFYSIFNPKIIKRSKEISGMEEGCLSVPDFR
                     DEAFRNEKITLEGFDKNNKKVKIKAWGLLACVFQHEVDHLNGKLFIDRTKAPK"
     gene            complement(3655..3834)
                     /locus_tag="VI432_00670"
     CDS             complement(3655..3834)
                     /locus_tag="VI432_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496648.1"
                     /translation="MCLDALGFSKVADATLDGMPTQFILEPKPSLWQDLSAMFRVCGL
                     KRDAVVPFHSWLVMG"
     gene            4346..4420
                     /locus_tag="VI432_00675"
     tRNA            4346..4420
                     /locus_tag="VI432_00675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Glu,seq:ttc)
     gene            4440..4514
                     /locus_tag="VI432_00680"
     tRNA            4440..4514
                     /locus_tag="VI432_00680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4474..4476,aa:Gly,seq:gcc)
     gene            complement(4612..4878)
                     /locus_tag="VI432_00685"
     CDS             complement(4612..4878)
                     /locus_tag="VI432_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496649.1"
                     /translation="MIDKEYLELLKKRSKESRVYSPHQMVGLLIAEILGDEDHKSLYM
                     RLAKNNNHDALIKLAKSVADRKNIKNKGAYFMRLWKSEIKNSKI"
     gene            4938..6149
                     /locus_tag="VI432_00690"
     CDS             4938..6149
                     /locus_tag="VI432_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4496650.1"
                     /translation="MTKNKIIIPVAIFIVAIFSYSIGFGYGSSQPKNIVIEGLKGGEN
                     PENIDADFKIFWEAWSELKSHHIDGGELNAKDFVYGAIEGLANSFNDPYTIFLDPEDS
                     KKFNEDVGGSFGGIGAEIGIRNEQLTVIAPLKNTPAEKAGLRAKDKIIKINDEITSGV
                     TIDDAVKSIRGVPGTKVTLTIFRDEWERSKDIEIIRQVIEVPTVDWKFVDDKLIHMQL
                     SSFNWNLIGAFSKALTESLGEGGRGLIIDLRNNPGGFLETAVDLAGWFLDKGSVVVTE
                     KFTSGKENIFRANGNASLKHFPIVILINGGSASASEIFAGALKHHLETKIIGEKSFGK
                     GTVQQLDQLSDGSSLKITIANWLMPNGEIIEKNGIEPDYPIEITDENIKNEEDPQLEK
                     AIEILNQEIKF"
     gene            6156..6611
                     /gene="rplI"
                     /locus_tag="VI432_00695"
     CDS             6156..6611
                     /gene="rplI"
                     /locus_tag="VI432_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY4496651.1"
                     /translation="MKVILLDNIKKIGKKHEIKDLSEGYIRNFLIPRGLAKIATPEAI
                     KEIEKIKFADINNLEDKKTSLNELRNKLQKNPLKIYIKTGEKHEIFSGIHKEDIIKAL
                     IKEKLISEKDEIEIDMDKPIKKLGEAQFEANLGRGVKGMLKLEVVPQQP"
     gene            complement(6580..6861)
                     /gene="rpmA"
                     /locus_tag="VI432_00700"
     CDS             complement(6580..6861)
                     /gene="rpmA"
                     /locus_tag="VI432_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY4496652.1"
                     /translation="MAHTKSGGSTKLGRDSASKRLGVKIQDGQNAYAGQIILRQRGTR
                     YLPGLNVRRAGDDTLYAAKNGTVKFLSKNKTRFDGSRRKVKVVAVQPQV"
     gene            7039..7185
                     /locus_tag="VI432_00705"
     CDS             7039..7185
                     /locus_tag="VI432_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496653.1"
                     /translation="MEKSTNQISLKLYIVLGFIFILSLSFAFGIGYKIGKELNSHPII
                     IENN"
     gene            complement(7459..8220)
                     /locus_tag="VI432_00710"
     CDS             complement(7459..8220)
                     /locus_tag="VI432_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496654.1"
                     /translation="MYKSNGNGDFKEKHGYIVEGRWYPRVTSILDIKAKPALYRFYGG
                     LESFARGEMIKQKSADEGTLLHQAAERVLTGQYDGGVDPRIEPSVITLIDFIKNTNIQ
                     VDADWVEKRVINFRDRYAGTIDALAVIDGKFGVLDIKTSQSIYRDYNLQTAAYFSPLS
                     ETFDGLQTRWILRVDQNQKCLRCGAKRRIKGGHEKIRKNGFNGDCLHDWSEMRGEIEL
                     KEFPHWYEDFEAFLAAKRLWEWENENSLKKIGYLL"
     gene            complement(8375..9082)
                     /locus_tag="VI432_00715"
     CDS             complement(8375..9082)
                     /locus_tag="VI432_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496655.1"
                     /translation="MNLRDSNKVYSSKKNPWTIEQVEAGLRHFFDASGRYPTATEIDD
                     YSFLPTNRTIERSFGGIIALRKKLRLGNEYDFRAGRHSSRRAHIINKRSNNLENEVYM
                     YLCDKFHKEFVHREYLFTDDARTRADFFVYVHDNDFLVDVFCPSSLRNLTLCVNVKLK
                     KYQNSPTLIGKTPLIFLQMNKSISQDKINEVLASKKRKYFLYKNQYVMDWDSFNKFCS
                     SKKSLNKVISNKDRYVA"
     gene            complement(9166..9303)
                     /locus_tag="VI432_00720"
     CDS             complement(9166..9303)
                     /locus_tag="VI432_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496656.1"
                     /translation="MEFFKGLFEELKDVGESLIPILKSLGSIFAKVLDFFANLIRSLI
                     T"
     gene            complement(9318..10610)
                     /locus_tag="VI432_00725"
     CDS             complement(9318..10610)
                     /locus_tag="VI432_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY4496657.1"
                     /translation="MRKASIFLIFFILGSGMAASQVDSSSPEALLSAINEKTKQLEDV
                     NIQIQETQKNVDILGREANTLNKEIKRFDYNISQMNLGIKSSEIKIEKLGLELEVLEN
                     NIETVTDEINLKRTAIGEALRRVQQNDGQSLIEILLKNRSLVEAVLEVQSLKDLQVGL
                     SSSVIQLGLSRSELDEVLQETAIKKVDVETENKNLKNRKSILDEQKNEKNQLLKEVKN
                     KESIYQEQITLLEKQQRALSEEIDKIEDDLRVRFDRALIPSPRPGVLSWPIKLVNDGG
                     IGRITQHAGEVSYLYGGRAHNGLDIGVPLGTPVFAAADGVVAAVDNNDVSSYRKYQYG
                     KYVLIKHDNNLATLYAHLSKQVVSAESAVKRGELIGYSGNTGYSTGPHLHFGLYGSPS
                     SIISCPANISIPMLCMGKRPPAAGLVPIGPYLDPEQYL"
     gene            complement(10607..11350)
                     /locus_tag="VI432_00730"
     CDS             complement(10607..11350)
                     /locus_tag="VI432_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY4496658.1"
                     /translation="MNNRFASRVFDPLSKEPFRISRSKIELFKDCERCLYLNLRYGVN
                     RPSTFPFTLNNAVDALLKKEFDIHRAKGKAHPLMERYGIDAVPFDHPKMEEWRDALRR
                     GIRHHHLQTNFIVSGGIDDLWINPADELIIVDYKATAKTSEVNIDADWQMSYKRQMEI
                     YQWLFRQNEFKVSNIGYFVYANGQKDKDAFDGVLEFDVQIIGYEGNDSWIPGVLGDIR
                     SMLENDKLPEASKDCEHCSYRERAREVEK"
     gene            complement(12129..12557)
                     /locus_tag="VI432_00735"
     CDS             complement(12129..12557)
                     /locus_tag="VI432_00735"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY4496659.1"
                     /translation="MAKNKIIIKTDGGARGNPGPAAIGVVIELSDGSKSKGYGEQIGN
                     TTNNIAEYKAVVFGLKKAKQLFGKIREGADVEVRSDSELLVNQLNGKYYVKNEDLFPL
                     FINIWNLKQDFGSVEFKHVRREENKEADRLVNRALDTLGI"
     gene            complement(12539..13369)
                     /locus_tag="VI432_00740"
     CDS             complement(12539..13369)
                     /locus_tag="VI432_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY4496660.1"
                     /translation="MKKIVIIYHKFCLDGFGGAWAAWRKFGNKATYIGMEHKSPAPGG
                     LKGKDVYMIDFSYKEQVIKKMLSEVKSLTIIDHHVSAEKVVKSLKNHLYAIDHSGAVL
                     AWKYFHPNKKVPKLLAYIEDGDLWKFRLKNAKELIAFLITQSLDFKNWNKLYKDFESA
                     DVRKEFVKRGVVIINYESKVIQKAIMTADLVKFEGYKVMASNSAFLISEIGHALVNKT
                     PPIGIIYSISGGSVKVSLRSNGKVDVSKIAVKYGGGGHKAASGFVFKLEDNFPWQKIK
                     "
     gene            complement(13366..14442)
                     /gene="pgk"
                     /locus_tag="VI432_00745"
     CDS             complement(13366..14442)
                     /gene="pgk"
                     /locus_tag="VI432_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4496661.1"
                     /translation="MKYLLRVNLDIKDEKIRESLRFKSVVFTVKKISKKNNKIIILSH
                     RGRPNGFDKKLSLKPIAKLLSEAIEKKIIFIPDFDFPSIKNRIDQSINGSILMIENLR
                     FLGGEQNNNRILSRTLAGLGDVFINDDFATAHRKNASNVGITEFIKSIKGPVFENEIK
                     SLTRVIRQPKHPFLLVIGGAKMSDKLGVMNNLIKRVDNVVLGGGVANTFLKAQGFDID
                     KSIFEPEVLGLAKKMAKNRKIIMPVDYKKIKDRILDIGPRTIKIYSKLIRDAKTIIWG
                     GPLGLYEDKRFSHGSFGVAKAISKSKAFAVVGGGETSQIIMRLGLEDKIDLVSTGGGA
                     MLDFLAGKKMPAIDALKNKNRNKK"
     gene            14581..14859
                     /locus_tag="VI432_00750"
     CDS             14581..14859
                     /locus_tag="VI432_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496662.1"
                     /translation="MIEESIRMKGLRKTTRSIREILTERGCANSHLNDDQLAELIRSM
                     LNGNGEPIKLRSTEGHKEFMEQLRRFWEYRERTIMDEKLTNAFGSAHH"
     gene            complement(14890..15444)
                     /locus_tag="VI432_00755"
     CDS             complement(14890..15444)
                     /locus_tag="VI432_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4496663.1"
                     /translation="MLKSKPLVIVGAVLLTLVLSYFYFNSEDRATLDNEYEFDGSIES
                     STDDASAVDGDNLVYSGAIIAGTAENPLLDFKKSDYDLALKNEENILLYFYANWCPIC
                     KNETTSGLYPAFNNLEGRDVIGFRINYNDNQVDGDEMDLAKKLGINYQHTKVLLKSGE
                     PILISLEFWDKSRYLTEIDRLINQ"
     gene            complement(15493..16905)
                     /gene="gltX"
                     /locus_tag="VI432_00760"
     CDS             complement(15493..16905)
                     /gene="gltX"
                     /locus_tag="VI432_00760"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY4496664.1"
                     /translation="MKDLIRVRFAPSPTGPLHVGGARTALFNWLFARSKGGKFILRIE
                     DTDRERSKKEFEESIVTGLEWLGLTWDEFYRQSERSDIYRDYLLKLIQSGEAYYCFCT
                     KEDLEAEKTAMKADGLSPKYSGRCRSISKEEAKSRVEKEKSVIRFVVPEKEVSFNDLI
                     KGDIKFNTSLIGDIVIAKSLDQPLFNFAVVVDDGLMEITHVIRGEDHIPNTPKQILIA
                     EALGFDIPRFGHMPLILNTDRSKMSKRFADVAMSDYIRAGYLKDAMMNFLAFLGWHPK
                     EEKEIMTRQELVDEFSLERMQKAGAVFNIEKLDWMNGYYINKLSDEDFIIYASNFVPS
                     GWKLNPEIAASVKSRIKKFNELRDLIDFYFELPDYDTTLLRWKESPLSMAREGLREIA
                     SRIEVVGDNNFNKDYLEKTLLEAVPKDKRGDFFWPLRVSLSGKKTSPPPFEIMGIIGR
                     TESLNRIKIAESKIAKNSGM"
     gene            complement(16917..18056)
                     /locus_tag="VI432_00765"
     CDS             complement(16917..18056)
                     /locus_tag="VI432_00765"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEY4496665.1"
                     /translation="MARKFRVLMTGGGTGGHIYPLLAVAIELQFLSVEEGIDLKLKYL
                     GSYGSYKELLSLNNIKVQKIVSSKLRRYFSISNFWDFFKFSYGVLQALWKIYWFMPDV
                     LFSKGGPGALPVVLAAKFYRIPIIIHESDVVPGMTNIVSARFADIITVAFSGAAKYLG
                     PASMPVGNPVRRYILPHGEDEDNKTAKKLLEFDSDKFLIFVVGGSQGSTRINDFIIEN
                     LGDLLQSSQILHQTGRNNYEQVVGQVAFEVEKMPEAIKKNYKAVDYFENGLGDAMLAA
                     DLIVARAGAGSIFEIAAFGKPSILIPLPESAGDHQRYNAYEYASTGASVVIEEENLLP
                     HLFMAQLQKLLEHPEELQRMGEAAKRFYKKDSALTLARLILDFNK"
     gene            complement(18096..19187)
                     /locus_tag="VI432_00770"
     CDS             complement(18096..19187)
                     /locus_tag="VI432_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEY4496666.1"
                     /translation="MRRSRKIDYVILVVILLLVSYGLLILSSASSDIGQTRFDNPYHY
                     LWRQIIYGLGLGLIGFLAGLYVPYAKYKKLAPLFLFLSLIVVILPFTPLGFSSGGASR
                     WINLGPFSFQPAEILKIFFIIYIAAWLSSYHKNRQAGFMEGLIPFLAISGFVSLILLL
                     QRSTSAMVLLMVGVLAVYFASGAKKLYILSIIVGGFVFVAATIYFTPYRLDRITSFLT
                     PHADVEGSSYQINQARITIGSGGLWGVGYGNSLSKEYLPERIGDSIFAIVAEEFGFVG
                     ATALMLLFLILITRGFILSKKIRDPFGKLLLVGFSTIIGFQAFVHIGSISGLIPLTGV
                     PLPYISYGGTALAVFMTIAGIMLNISKNA"
     gene            complement(19306..>20316)
                     /locus_tag="VI432_00775"
     CDS             complement(19306..>20316)
                     /locus_tag="VI432_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4496667.1"
                     /translation="EERDLTDKETEVFKRILNKALYEGAMGLSSGLGYAHGRHISNDE
                     LKRLVGVVKESDAIYSTHLRNETNNLLDSVEETINIAKETGAKVLISHLRPLNGYESK
                     FDKAMELIDKNLDSANIYFYVYPFDISLNSIYTLLPIWAQERNLETMLYNIRNSEMAL
                     KIERDMPEFNPNDIKIVDAPKNDYLVGKTIAQSAENLGLSHKAALLKVMDITGLKAVV
                     ANRNINFDYLVKLLPRERVLIASNSDSRRSSSDFVKNERSLNTFPRYLKLAVQNNSML
                     IEEAIKKITMKPAELFGLKKRGVVKERYIADLVLINKSDFGIIRTIVGGGIGGQILRH
                     YK"
BASE COUNT         6439 a         4423 c         3316 g         6138 t
ORIGIN      
        1 tgatgctctt agaataataa cctcggccga ataataacta tagcaaacca tagatacatg
       61 cgaatctaca aaaaatcatc agcgaataat gaataactat acgaatagaa attcggagca
      121 ttcgcctgtc taccaaggca agcataaatt ctatatttca aatcaaagcg gtttaactct
      181 tattgaaact cttatttact caattctggt tgccggactt ttagccgggg ctttgatctc
      241 agtttatcaa cttcttgagg ggagtgaaga actaaacctg ataacaacaa ctgaagaaga
      301 agcaaatttc atcctgcgta aaatccaatg ggctcttact ggaatagatt ctataaattt
      361 tcccggtcct ggggatagcg actctatcct tattacaaat aaaataaatt atgctcaaaa
      421 ccccatactc ttccagcttg atggaacaga tattaaaatg agcaggggag ggggatctct
      481 aaactctctc aattcaagcc gagtcacaat agagaactta ttatttactc atctcgcatc
      541 agccggagaa aaaccggccg ggattaaaac tgagatagtc ataaatggcg ctgaatacga
      601 aactttaatt tattttaaat aaatgcaaaa gggatatata gctcttacat caacaatagt
      661 tatttctatc ttaatactca cgataattct cgcggtcagc ttcaccgggt tttccacaag
      721 atcaaatctt ttgaattatt attataaaga gataacccgt tcggtcgcag aaggatgtat
      781 aaacacagcg cttagtaaat ctgcccaaga tcctctatac gagggaaatg aaacaatcga
      841 tataggacaa gacacatgca tgatttatcc tattgaaaac gatattccaa atattcaaaa
      901 agttatcaag gcgaaggccg tgttaaaaag agttgcaaca aatttaaaag tgaccgttaa
      961 tcttcttact cttgatatta tcgcctggga agagattgaa aatctttaaa aataaatatg
     1021 ttataatatt tccagtttaa tgaaaggtcg agattttatt agtaatttaa ttgaaatgaa
     1081 aaatcaaaaa ggatttacgc ttatcgaagt tcttatcgtt ataggcatta tcgctgtttt
     1141 agcgggtata gttatcgttg ctataaatcc ggctaggcaa tttgcgcaag gaaataacac
     1201 tacaagacgc agtcacgtca actctatatt aaacgctatc ggacaaagaa tagctgataa
     1261 tagaggccta tttgagactg gatgtgccgc tggagctata ccagcaaatg ctactcttat
     1321 ggcagaagat gcagacaatg accctctaac cgttgaatac gatatagcaa actgtcttgt
     1381 tccaacctat cttccatcaa tgccgtttga tccgatagga gatcaaacag acggaaccgc
     1441 agactgggta gattccgacg actacaatac cgggtacacc gtgcttcaag atgctgacgg
     1501 cagaataacg gtcgccgcgc caagcgcgca gcttaatgaa gtaatatctg ttacaaggta
     1561 aaacgaatag ttttatctct ttattaaaac aactcccgcg cgggagttgt tttttatatt
     1621 gcgcgcggct tctttccttc ttcttggatt ttaagaagtt ttaaccgtcc atcttcgttt
     1681 attgcatcca atagcttaat tcgtttgccg tctaaaataa tatatgcccc cggctccgga
     1741 ttaaaagctt ttatcattcc taaaattttt actgttttat caacatctcc gctctcagct
     1801 atttttaaat ctccaagatc aatcaacgcg tcttcggtat taaacttatg cgtataagtc
     1861 gcctctgatt cattttgagg aatcggggtt atttctccac gtataaaacg cggcagcatt
     1921 tcaactaaca tgtctccgcc caaccaagca agatcttttt ctaattctaa gtaattttga
     1981 tttttgattt ctaaattatc tgttttaagg acaggtccgt gatccatctt ttcatccatt
     2041 agatataagg acacccctgt ttcctctttc cctgaaagta gcacagattg gataggggat
     2101 gatccacgat atttcggcaa gagcgaaggg tgaactccta tcactccacg aaaggggatg
     2161 tccaaaagtt ctttcggcaa aatttttgcg tatgccgcaa caataaaaaa atctgctttc
     2221 agctggcgta atctattcaa aaaactttga tcaatggttt ctggctgtaa aatttcaaca
     2281 ttgatcgact gcttatctat tgttattaat cgttcaacta actgcttagt tgccggcggt
     2341 gtaataatct tcttcctacc aaccggccga tctggattgc atactaaagc agtcggaata
     2401 aacccatttg taattaattt atctaaaact atttctgcga atctcggaga accaaaaaat
     2461 atatagtgca taacaataat actaatatac gaacggtact aatgatacta atcgatacga
     2521 cttattcgta tgtattcgtt gcatcgagat aaattatgca tattggttat ttcactttgg
     2581 cgccttagtg cggtcaataa ataatttacc attcaaatga tcaacctcat gctggaatac
     2641 gcaagctaaa agtccccaag ctttgatttt cactttcttg ttgtttttat caaatccctc
     2701 caatgttatc ttttcgtttc taaaagcttc atctctaaaa tcaggaacgc ttaaacatcc
     2761 ctcctccatg ccagaaatct cttttgatct tttaataatt tttggattaa aaatagaata
     2821 aaattttccg tcaacccttg cgacaaaaac attcatatcc aagcctatct gattggcggc
     2881 gagcccaatg ccttcagctt catccattgc attcttcatt tttattatca aatcatgcac
     2941 ttctttttta ctaaatctat caaaatcaaa atccgcggtc tttttgcgca gaaacctctc
     3001 ttctttttta tctttaatag taacaatttt aacagtcatt taatctagaa attgtaacac
     3061 aaagaaggat aaaaatagga catcaaagag tagattattc gcttgacata ttcaaataaa
     3121 atgataaaat atttgccaat caatacgcac attaataatt aagactcaaa tggctgtaaa
     3181 cagccccctc gtagtttagc gatggcttag gaataaacat acatacctaa agaagccaaa
     3241 gaacatcctg ttttggaaaa tcgctcatcg gccacagatt ccttggggag gttgcttctc
     3301 aaaaagaggc aggcgattag gcacaccctt aaagccgtct gtggttatcc aaaacgcgcg
     3361 cagacctccg taaatcggag cacggctgta tgcgttccca cagccaatag aatgcgcatc
     3421 tcgaaaaatt atcttggtga cgatcaaaaa acttgcagca ccaaaaactg gttgtcaacg
     3481 agatgaaagt agggatagga aggcgtcggg cgatccgacc gagggctcat cctcacaaga
     3541 agcgacttta tgtcgcttca tttttttaat aaaacgcttt gacaaaaagc attaggtaag
     3601 taatatatta gttatcgcac ctttaatatt tcatatggcc ctcgttagca taagctaacc
     3661 cataaccaac cagctgtgaa acggaacaac cgcatctctt ttcagaccgc acacgcgaaa
     3721 catggcagaa agatcctgcc agagagacgg tttcggttcc agaataaact gcgtaggcat
     3781 gccatctaaa gtggcatctg caaccttgga gaagcccaat gcgtctagac acatttgcct
     3841 cggccaccta aactccgaaa cgagctctaa acaggagaag tggcgagaat tccaacaaac
     3901 ccgattatgg gttcgtgacg cactgaccag tatccttgca gaagggagtc gttagatccg
     3961 gccgagggcc caatctcttc ccactcaata agagcgattc aaatcgctct ctttttgttt
     4021 acagcttaaa aattgacatt gcttcgtata gataataaaa tataaatcct ttatcgttct
     4081 ttcccaaaac aaaatgctcc cgtagcttag ctcggttaaa gcacccatac cccactcgca
     4141 taacgggcat atgctcggtc aggcaaataa agcggaagaa gaccatctct tcgttagacc
     4201 gctgaaagta agccgccaca ccgcagacgt tgccattcgg aacccgatgg ggcatccctt
     4261 ggccgagagt gcgtctgcga caccgagacc gcaaacaatg ggaatgggat atcgcaggtt
     4321 caaatcctgc cgggagcatt caaatggact cgtagtgtag cttggtctat catgcacgcc
     4381 tttcaagtgt gtaaccctgg ttcaaatcca ggcgagtcca ttttactctt tcccaaaacg
     4441 gggttgtagc ttagtccggc atagcgtctg gtagccatgc cagaagatcg caggttcaaa
     4501 tcctgccaac ccctttccaa cgacttgtct cataacgaaa ctagtcgttt ttctttttta
     4561 tttcatcccc cttttatccc catggggata aaagggggat gaagctttta ttcaaatctt
     4621 cgagttttta atctctgact tccaaagccg cataaaataa gctcccttat ttttgatgtt
     4681 ttttctatcg gcaacagact ttgcgagctt gatcaaggca tcatgattat tattcttagc
     4741 cagcctcata tacaaagatt tatggtcttc atctcccaag atctctgcaa ttaacagccc
     4801 caccatctga tgaggagaat aaaccctgga ttctttagat ctctttttaa gcaattccag
     4861 atactcttta tcaatcataa atatattaaa acctatcgtt gatattatat ttaaaatcct
     4921 ttactatagc tattatcgtg actaaaaata aaataattat tccggttgca atattcatag
     4981 tagcgatatt ttcttattct atcggcttcg gatacggctc ctcgcaaccc aaaaacatag
     5041 ttatagaagg gctaaaaggc ggtgaaaatc ccgaaaatat agacgctgat tttaagatat
     5101 tttgggaagc atggtcagaa cttaaaagcc accatataga cggaggcgaa ttaaacgcca
     5161 aagattttgt ctatggagca atagaaggac tagcgaactc atttaatgat ccttatacaa
     5221 tctttttgga ccccgaggat tctaaaaaat ttaatgagga cgttggtgga agtttcggag
     5281 gaatcggcgc agaaatagga attagaaacg aacaacttac agtaattgct ccgcttaaaa
     5341 atactcccgc cgaaaaagca gggcttaggg ccaaagataa aattattaaa atcaacgatg
     5401 aaataacatc tggtgtaaca attgatgacg cggtcaaatc aataagagga gtgcctggca
     5461 caaaggtaac tctcaccatc ttcagagacg aatgggaaag atctaaagat atagaaataa
     5521 taagacaagt aatagaggtg ccaacggttg attggaagtt tgttgacgac aaattaattc
     5581 atatgcaatt gagtagtttc aattggaatt taatcggcgc attcagtaaa gcgcttaccg
     5641 aatcgcttgg agagggcggc agaggattaa taatcgatct aagaaacaat cctggcggct
     5701 ttttggaaac agctgttgat ttagctggat ggtttttgga taaaggatct gttgtcgtaa
     5761 ctgaaaaatt tacgtctggc aaagaaaaca tattcagggc aaatggaaac gcctcactca
     5821 aacattttcc tatagtgatt ctgataaatg ggggatcagc atctgcttct gaaatatttg
     5881 ccggagcatt gaaacaccat cttgaaacta aaatcatagg agaaaaaagt ttcggcaagg
     5941 gaacggtcca acagcttgat caattaagcg atggctcatc attaaaaata accattgcta
     6001 attggcttat gcctaacgga gaaatcatag aaaaaaacgg catagaaccc gactatccaa
     6061 ttgagataac agatgaaaac ataaaaaacg aagaagatcc acagctagaa aaggctattg
     6121 agatattaaa tcaagaaatt aaattctaaa aaataatgaa agttatccta ttggataata
     6181 ttaaaaaaat cggcaagaag catgagataa aagatttgtc tgaaggatat ataagaaatt
     6241 ttcttattcc gcgaggactt gccaaaatcg ccacaccgga ggcaattaaa gagatagaaa
     6301 aaattaaatt tgcggacata aacaatcttg aagataaaaa aaccagtctt aatgaattga
     6361 gaaacaagct tcaaaaaaat ccacttaaaa tttatataaa gaccggcgaa aaacatgaaa
     6421 tcttcagcgg gatacacaaa gaagacataa tcaaggctct cataaaagag aaattgatat
     6481 cagaaaaaga cgagatagaa attgacatgg ataaaccgat aaaaaaactg ggcgaggccc
     6541 agttcgaagc aaatctagga aggggagtaa aggggatgct taaacttgag gttgtaccgc
     6601 aacaacctta acctttctcc tgctgccgtc aaaacgagtc ttatttttag aaaggaattt
     6661 aacggtacca ttttttgcgg cgtataaagt gtcatctcct gcgcgcctta catttaatcc
     6721 cggcaaatat ctagtgcctc tctgacgaag aataatctgt ccagcataag cgttctgtcc
     6781 gtcttgaatc ttaacaccta agcgttttga tgccgaatct cggcctaatt ttgttgaacc
     6841 tcctgacttt gtatgtgcca ttattattta aatttattta ataaatttta ctataataat
     6901 gagtatccgc ctcttgcaga tagcccacca tttttattca gcgagaaaaa tgagcggccc
     6961 cttatcccaa aacttaatgt atggtatcag cgcgccattc ccagctaatt tagtatgaat
     7021 tataatatag taaaactgat ggaaaagtca acaaatcaga tctctctaaa attatatata
     7081 gtgcttggtt ttatatttat tttaagctta tcttttgcct tcgggatcgg ctataaaatt
     7141 ggcaaagaac taaatagcca cccgatcatc atagaaaata actagctttt atgattcctt
     7201 gacaaactat ataaatatgc tattataata gcgtaagtca ttgttcatta tcatatccat
     7261 cttaaatgcg tccttcgcgg gccgtcgcgg gctatgcggc agggatctgg agtgctgtga
     7321 ttcgaccatc acagactccg gattcctgcc cacccggacg gaaacctgca ttagacgcgt
     7381 caccaagagc ctcatgagaa cagctttctc acccgaggct ctttcacttt ttaaaaacta
     7441 ttgagactct tttggggttt ataaaagata tccgatcttc ttcaaagaat tttcattttc
     7501 ccactcccaa agacgcttag ctgcaagaaa ggcctcaaaa tcttcatacc agtgggggaa
     7561 ttccttaagc tcaatttcgc ccctcatttc cgaccaatca tgcaaacagt ctccgttaaa
     7621 accgtttttt cttatttttt cgtgtccgcc tttaattcta cgttttgccc cgcatcgcaa
     7681 acatttttga ttctgatcta ctcttaaaat ccaccttgtc tggagaccgt caaaagtttc
     7741 cgaaagagga gaaaaatatg cagcggtttg aagattataa tctcgataaa tcgactgaga
     7801 agttttaata tctagcaccc cgaatttacc gtctatcaca gctaaagcgt caatcgtacc
     7861 cgcatagcga tctctaaaat ttattactcg tttttcaacc caatcggcat caacctgaat
     7921 attggtattt tttataaaat cgatcaaagt tattacggac ggttcaatcc ttggatcaac
     7981 acctccatca tattgtccgg ttaaaactct ctcagctgcc tgatgcaaaa gagttccttc
     8041 gtcagccgat ttttgtttaa tcatttcacc acgagcgaaa ctttccaaac cgccataaaa
     8101 tctataaagc gctggcttag ctttaatatc taatatactt gtaaccctgg gataccaacg
     8161 gccctcaacg atatatccat gtttttcttt aaaatctcca tttccattag atttatacat
     8221 attcgacata aaaagcgctt atagagcaaa tttaaagcaa attttacacc ataataaagg
     8281 ggatataaag ggcttcatga gaataaacaa tggtagggaa tccaaaccaa aggttattag
     8341 aagcgtcgca taatatacct ttttatcaga tagtttatgc gacatatcta tctttgttag
     8401 aaatcacctt attcaaagat ttcttagatg aacagaattt attaaaacta tcccaatcca
     8461 ttacatattg atttttatat aaaaaatatt tgcgcttctt gctcgccaag acttcattga
     8521 ttttatcttg ggaaatgctc ttattcattt gtaaaaatat tagaggagtt tttccgatta
     8581 atgtgggtga gttttgatat ttctttaatt ttacatttac acaaagagtt aaattgcgca
     8641 acgaacttgg acagaataca tctaccaaaa aatcgttatc atggacatat acaaaaaaat
     8701 cagcccgcgt acgagcatcg tctgtgaaaa gatattccct gtgaacaaat tctttatgaa
     8761 atttatcgca gagatacata taaacttcat tttctaaatt atttgatctc ttgtttataa
     8821 tatgtgcgcg ccttgaacta tgtcttcctg cccggaaatc atattcatta ccaagtctaa
     8881 gtttcttacg taaagctatg atgcctccga aacttcgttc tatcgttcga ttggtcggta
     8941 aaaaagaata gtcatcaatt tctgttgcag tcggatatcg tccgcttgca tcaaaaaaat
     9001 gccttaaccc agcctcaact tgttcaattg tccaaggatt tttcttacta ctgtaaactt
     9061 tattagagtc tcttagattc acaaactaat tataatacat aatgtcgcat aagcgataat
     9121 taaatatacc gctaagaact aacaattaac atctgatgtc ttgaatcagg ttattaaact
     9181 tcttattaga ttagcaaaaa aatctaagac cttagcgaat atagaaccga gagacttcag
     9241 aataggaatt aagctttcac caacatcttt caattcttca aaaagtccct taaaaaattc
     9301 catttgattc aaaaatacta taaatattgc tctgggtcaa gatacggtcc gatcggaact
     9361 aaacctgcgg ccggcggccg tttacccata cataacatag gaatgcttat atttgctgga
     9421 cagcttataa tagagctcgg agatccataa agaccaaaat gcagatgcgg accggtagaa
     9481 tatcccgtat tgccggaata accaataagc tctcccctct taaccgcact ctccgcagaa
     9541 acaacttgtt ttgataaatg agcatataaa gttgccagat tattatcatg ttttattaga
     9601 acgtatttgc catactgata tttcctgtaa gaacttacgt cgttgttatc aactgccgcc
     9661 acgacgccat cagcggctgc gaaaaccgga gttccaagag gcactcctat atcaagcccg
     9721 ttatgagctc tgcctccgta aagataagaa acctctccag cgtgttgagt aatcctgccg
     9781 atgccgccat cgtttactaa ttttatcggc cacgataaaa cgccgggtct aggcgacggt
     9841 ataagcgccc tatcaaatct tacgcgtaaa tcatcttcaa ttttatcaat ctcttccgaa
     9901 agagctctct gctgtttttc taataaagtt atctgttctt gataaataga ttctttattt
     9961 ttcacttctt taagaagctg atttttttca tttttttgct catctaaaat agatttccta
    10021 ttcttcaaat ttttgttctc cgtctcaaca tccactttct ttatagcagt ctcctgaaga
    10081 acttcatcca gttcagaacg cgataaacca agctgtataa cactagaaga taaaccaacc
    10141 tgtaggtctt ttaggctctg aacctcaaga accgcctcta ccaaacttct attcttcaat
    10201 aaaatttcta tcaaactttg tccatcgttt tgctgcactc tcctcaaggc ctctccaata
    10261 gctgtccgct taagattgat ttcgtcagta acagtctcta tattattttc caaaacctct
    10321 aactctaacc ctaatttttc tatctttatt tcactagatt ttattcctaa attcatttgg
    10381 ctaatgttgt aatcaaatcg tttaatttcc ttattcaaag tgttagcctc tctgccaagg
    10441 atatcaacat tcttttgagt ctcttgtatt tgaatgttaa cgtcttcaag ttgtttcgtt
    10501 ttttcattaa tagcgcttag aagagcttcg ggcgacgaag aatcaacttg gctagccgcc
    10561 ataccggatc cgagaataaa aaatattaaa aatatagacg cttttctcat ttctctactt
    10621 ctcttgctct ttctctgtac gaacaatgtt cacaatcctt actcgcctcg ggtaatttat
    10681 cgttttcgag catactcctg atatcgccca aaacgccggg aatccaacta tcattaccct
    10741 cgtatcctat aatctgcaca tcaaactcca aaacaccgtc aaaagcatct ttgtcttttt
    10801 gaccgttagc ataaacaaaa tatccaatat tcgaaacctt gaattcattc tgccgaaaaa
    10861 gccactgata gatctccatc tgacgcttat agctcatctg ccaatcagcg tcgatattaa
    10921 cttcgcttgt cttagccgtc gctttgtaat cgacgatgat taactcgtct gcgggattaa
    10981 tccatagatc gtctattcct ccggaaacta taaaattcgt ctgcagatgg tgatgcctga
    11041 ttccgcgccg taaagcatct ctccactcct ccattttggg atgatcaaat ggcacagcat
    11101 caattccata tctttccatc aatggatggg ctttaccttt agctcggtga atatcaaatt
    11161 ccttctttaa tagagcgtca acggcattat tcaaagtaaa tggaaaagta gatggccggt
    11221 ttacaccata tctcaaatta agatataaac atctctcgca atccttaaaa agctctatct
    11281 tgctccggct tatcctaaag ggctccttag acagaggatc gaaaaccctt gaagcaaatc
    11341 tattattcat aacctcgatt ttacatcaat tggcgttttt tgaaagtcag ctacataaaa
    11401 tccccgaaga attttcgggg attttatgtc cgggcagctc ataagccgga ttctgtgcca
    11461 atctagtcac gagctacgcg agtgttacta gattgagcac cctgagcgaa gtcgaaggat
    11521 tatccttcga taaactcaag acgctcaatt ttgtagtcgc tcgcgtaact cgcgaacaaa
    11581 atcggtgtaa taatctatct gggccattga ttgctcgctg gctcttgcga ctctctatca
    11641 atgctccgca ttggaagcac gaatatctaa atccgaagca cgaaacaata tctaaatccc
    11701 aaatattcaa atttttaaac atttggagtt tttgaattta tttcggattt cgagattaga
    11761 atttcgaatt tccagtgcga agcactggta gcacggtctt gcatccagta aggatttagc
    11821 cgtttcacct caacctttta tccctatagt tgagttaagc cttctagctt gggatagggc
    11881 ttctctgatc ctttcgaatc aaagcgtcac tgctcgcacc tctatgcttt cgcacgacgg
    11941 ccgttagccg ctacttttgc caattatggc agatgtccgg actttcctct ccaacatcgt
    12001 tatcgttaaa ttaaaagaac aggaattaag ggttatttaa cgacaacagc cgaagctatt
    12061 acctgaactg cccgtaaaag catttatacc acaaaagtta atttatgcaa gagcgataaa
    12121 atgacatctt atatgccaag tgtatccaaa gccctattta caagtctatc agcttcttta
    12181 ttctcttccc tcctcacatg tttaaattca accgaaccaa aatcctgctt aagattccat
    12241 atatttatga ataaaggaaa taggtcttcg tttttaacat aatattttcc attcaactgg
    12301 ttaaccaaaa gttcgctatc acttctcacc tccacatcag ctccttctct tattttgcca
    12361 aaaagttgtt tggccttttt taacccaaat acaaccgcct tatattcggc gatattattc
    12421 gtcgtattcc ctatctgctc accatatccc ttactcttac ttccatctga aagctcaata
    12481 accacaccta ttgcggccgg cccaggatta cccctcgctc caccatctgt cttaataatt
    12541 attttatttt ttgccatgga aaattatcct ctaatttgaa aacaaaacca gaagccgcct
    12601 tatgccctcc cccgccatat ttaaccgcga tctttgaaac gtcgacctta ccattcgatc
    12661 tgagcgacac cttaaccgat ccaccggata tagaataaat aatccctatt ggaggcgttt
    12721 tattgaccag cgcgtggcct atctctgaaa tcaagaacgc agaattagac gccataactt
    12781 tataaccttc aaatttgacc aaatcagcag tcataatggc tttttgtata acctttgact
    12841 cataattaat aatcaccacg cctcttttca caaattcttt acgaacatct gcgctctcaa
    12901 agtctttata caacttattc caatttttaa aatctaaact ttgagtaata agaaaggcaa
    12961 tcaattcttt ggcgttttta agcctaaatt tccaaagatc tccatcttca atataagcca
    13021 aaagtttagg cactttttta ttaggatgga aatatttcca agcgagcacg gctccagaat
    13081 gatctatcgc atataaatga tttttcaagc ttttaacaac cttctctgcg ctaacatgat
    13141 gatcgattat agtaagcgat ttaacctcgc taagcatttt tttgataacc tgctctttat
    13201 aactaaaatc tatcatataa acatctttac ccttcaatcc acctggcgcc ggagatttgt
    13261 gttccattcc aatataagtc gctttattgc caaattttct ccaagcagcc caagcgccac
    13321 caaaaccatc caaacaaaat ttatgatata taataactat ttttttcatt tcttatttct
    13381 atttttattt ttcagagcgt ctatcgctgg catcttttta cccgctaaaa aatctagcat
    13441 cgctcctccg ccagttgaga ccagatcgat cttatcttcc aaaccgagcc tcataataat
    13501 ttggctggtt tcgcctccgc caacaacggc aaaagcctta gatttcgaaa tcgcttttgc
    13561 aacgccaaaa gaaccgtgtg agaacctttt atcctcatat aagccgagcg gtccgcccca
    13621 gattatcgtt ttcgcatctc ttataagctt tgaatagatt tttatagttc tgggccctat
    13681 atccaatatt ctgtctttta tttttttata atcaaccggc ataatgattt ttctattttt
    13741 agccattttc tttgcaagac ccaaaacctc aggttcaaat atcgatttat caatatcaaa
    13801 tccttgagcc tttaaaaagg tgttcgccac gcctccgcct aaaaccacat tatcaaccct
    13861 ctttattaaa ttattcataa ccccaagttt atccgacatt ttagctcctc caattacgag
    13921 caagaatgga tgcttgggct gacggataac cctagtcaag ctcttaatct cattttcaaa
    13981 aacgggcccc tttatagatt taataaattc tgttatgccg acattggaag cattcttccg
    14041 atgggcagtc gcaaaatcat cattaatgaa aacatcgccc agaccggcta atgtcctgga
    14101 taaaattcta ttattattct gctcgccgcc taaaaacctc aaattctcta tcataagaat
    14161 tgatccgtta atagactggt caatcctatt cttgatagat ggaaaatcaa aatccgggat
    14221 gaaaattatt ttcttttcaa ttgcttcaga taacaacttt gcaatcggtt taagacttaa
    14281 ttttttatca aacccattcg gcctgcctct atgacttaaa ataattatct tgttattctt
    14341 tttacttatt tttttgaccg taaaaacaac agatttaaat cttaaagatt ctcttatttt
    14401 ctcatctttg atatctaagt ttactctaag cagatacttc atttgattaa tacattatat
    14461 cccaatatat caaaactaaa taactcattt ctcattcttt tttgacacaa tgataaaaat
    14521 attataattt cgaataagcg atcctttaag aatggatctt taacagcgag gctaaaaaca
    14581 atgatcgaag aaagtataag aatgaaaggg ctaaggaaaa caacccgttc aatccgcgaa
    14641 atcctaacag aacgcggttg cgcaaacagc cacctaaacg acgatcaact agctgaactc
    14701 atcaggtcca tgctaaatgg aaatggcgaa ccgattaagc ttaggtcgac tgaaggacat
    14761 aaagaattta tggaacagct aagaaggttt tgggagtaca gagaaagaac aatcatggac
    14821 gagaagctaa caaatgcctt cggatccgcg catcattgaa acgcaaagca ggccttcggc
    14881 ctgcattttt tattgattaa ttaatctgtc tatttccgtc agatatcttg atttatccca
    14941 aaattcaagc gaaatcaaaa taggctcgcc actcttaagt aaaactttag tgtgttgata
    15001 gtttatgcct aatttcttcg ctaaatccat ctcatcgcca tcaacctgat tatcgttata
    15061 attaattcta aaaccaatta catctctacc ttctaaatta ttaaatgccg gataaagccc
    15121 agaggtcgtt tcgttcttgc atataggaca ccagttagca taaaaataaa gcaggatatt
    15181 ttcctcgttt ttaagagcta aatcataatc agatttttta aaatctagaa gagggttctc
    15241 cgcggtaccc gctataatcg cacccgaata aacgaggttg tcaccatcga ctgcagaagc
    15301 atcatcagtt gatgactcta ttgaaccgtc aaactcatat tcattatcga gcgtagccct
    15361 atcttcggaa ttaaaataaa aatagctgag aaccagcgtt aataatactg ctccaactat
    15421 aacaagcggt ttcgacttaa gcattagaat ttatttatct ttattatata aattaaaaac
    15481 ccgactgtca gctcacatgc cactattctt agctatcttt gattcagcta ttttaattct
    15541 atttaaagat tctgtcctgc ctattatccc cataatttca aatggtggag gcgatgtctt
    15601 tttacccgat aaagacactc taagtggcca gaagaaatct cctctcttgt ctttaggaac
    15661 cgcttctaaa agtgttttct ccagataatc tttattaaaa ttattatctc caacaacctc
    15721 tatgcgcgac gcaatctcac gcagaccctc tcgggccata gacaagggag attctttcca
    15781 tcttaaaaga gtagtgtcat aatctggtaa ttcaaaataa aaatctatta agtctctaag
    15841 ttcgttgaat ttttttattc tcgatttcac ggaagccgcg atctctggat ttaatttcca
    15901 cccagaagga acaaagtttg aggcataaat tataaaatct tcatcactaa gcttgtttat
    15961 ataatatcca ttcatccaat ccagcttttc aatattaaaa acagcgccgg ccttctgcat
    16021 tctttccaaa gaaaattcat caactaattc ttgtctggtc attatttctt tttcctcttt
    16081 aggatgccac cccaaaaaag ccaaaaaatt catcattgca tcttttagat agccggctct
    16141 gatataatca gacatcgcca catcagcaaa cctcttagac attttgcttc tgtctgtatt
    16201 taatataagc ggcatatgtc cgaatcgagg aatatcaaaa ccaagagctt ctgcaataag
    16261 aatctgttta ggcgtgttag gaatatgatc ctcacctctt atcacatgtg ttatctccat
    16321 caaaccatca tcaacgacaa ctgcgaaatt aaaaagcggt tgatctaaag atttagcaat
    16381 aactatatcc ccgattaaag atgtattaaa ttttatatcg cccttaatta aatcattaaa
    16441 agagacttct ttttccggca cgacaaatct aatcactgat ttttcttttt ccactctgga
    16501 tttagcttct tctttagaaa tagacctgca tcttccgcta tatttaggag agagtccatc
    16561 cgccttcatg gccgtttttt ctgcctccaa atcttcttta gtgcaaaaac aataataagc
    16621 ttctccagac tgaataagtt tcaaaagata atctctatat atatcgcttc tctcactctg
    16681 acgataaaat tcatcccaag ttaaacccaa ccactctaaa ccggttacaa tactttcctc
    16741 aaactctttt ttactgcgct ctctatcggt atcctcaata cgaagaataa atttacctcc
    16801 cttactgcga gcaaaaagcc agttaaaaag ggctgttctt gctccgccaa catgaagcgg
    16861 tccggtagga gaaggcgcaa aacgcactct tattaaatct ttcatatatc aattatttac
    16921 ttattaaagt ctaggataag acgggctaaa gttaaagccg aatctttttt atagaacctt
    16981 ttcgcggcct cacccattct ctgaagttct tctgggtgct ctaataattt ttggagttgc
    17041 gccataaaaa gatgaggtaa tagattctct tcttctatta cgactgatgc gccggtagaa
    17101 gcatactcat aagcattata tctttgatga tctcctgctg attcgggaag cggaatcaat
    17161 atagaaggct tgccaaaagc agcaatttca aaaatagaac ctgctccagc ccttgccaca
    17221 atcaagtcag cggcaagcat agcatcaccc aatccgtttt caaaataatc aaccgcctta
    17281 taattttttt taatcgcctc aggcatcttc tcaacctcaa aagctacctg tcccacaacc
    17341 tgctcataat tatttctgcc ggtctgatgg agaatctgag atgactgaag caaatctcct
    17401 aaattctcta ttataaaatc gtttatacga gtcgaaccct gagacccgcc aacaacaaaa
    17461 attaaaaact tatccgaatc aaattccaaa agcttcttag ctgttttatt atcttcatcc
    17521 tctccatgag gaagaatata tcttctaacg gggttcccca ccggcatgga tgctggaccc
    17581 aaatatttcg ccgcgccgga aaaagcaacg gttataatgt ccgcaaatct agctgaaaca
    17641 atgtttgtca ttcctgggac aacatccgac tcatgaataa ttattggaat cctataaaat
    17701 tttgcggcta aaacaactgg gagcgctcca ggaccgcctt ttgaaaaaag aacgtctggc
    17761 atgaaccaat atattttcca taaagcctga agcactccat acgaaaactt aaaaaaatcc
    17821 caaaaattag aaatagaaaa atatcgcctc aatttgcttg aaactatttt ttgaaccttt
    17881 atattgttca gagataaaag ctctttatac gacccataag aaccgaggta tttaagctta
    17941 agatcaatcc cctcctctac agataaaaac tgcaattcaa tcgcaacggc aagaagcgga
    18001 tatatatgcc cgcctgttcc tccgccagtc attaaaaccc tgaattttct cgccatgcta
    18061 ttaatattac tttagattta taaaacctga tacatctaag catttttgct tatgtttaac
    18121 ataattcccg cgatagtcat gaagacggcc aaagccgttc ctccataact aatatatggc
    18181 aacggcacgc cagtaagagg aatcaaacca gaaatggaac ctatgtggac aaacgcctga
    18241 aaaccaataa tagttgagaa accaacaaga agcagcttgc caaagggatc tctgattttt
    18301 ttagaaagaa tgaagcctcg cgttataaga atcaaaaata gaagcattaa tgctgttgcg
    18361 cccacaaaac caaactcctc agccacaatt gcaaatattg aatctcctat ccgttctgga
    18421 agatattcct ttgagagaga attaccatat ccaacgcccc aaagaccgcc ggaacctatt
    18481 gtaattcttg cttgatttat ctgataactc gacccttcga cgtccgcatg aggcgttaga
    18541 aaagaagtta ttctgtccaa cctatatgga gtgaaataaa tagtcgccgc aacaaaaaca
    18601 aatccaccta cgatgataga taaaatatat aattttttgg cgccgctcgc aaaataaacc
    18661 gccagaacgc caaccatcaa taaaaccatc gcgcttgtag agcgttgcaa caaaagaata
    18721 agcgatacaa aaccggatat agcaagaaat ggaattaatc cctccatgaa tccagcttgt
    18781 ctatttttat gatagctaga aagccatgct gcaatataaa ttataaaaaa tatttttaat
    18841 atctcagccg gttgaaaact aaaagggccc agattaatcc accttgacgc tccacctgaa
    18901 gaaaaaccta aaggggtaaa aggaagtatt acgacaataa gacttaaaaa taaaaagagc
    18961 ggagccaatt ttttatattt agcatatggg acatataaac ccgccaaaaa cccgattaaa
    19021 cccaatccca aaccatatat tatctggcgc cataaataat gatatggatt atcaaaccta
    19081 gtctgaccga tatctgacga cgccgacgaa agaatcaaaa gtccgtaaga aactagaaga
    19141 agtataacga ccaggataac ataatctatt tttctacttc ttctcattcc gtttacaaat
    19201 ataacaaagt tattcttctc actacgcggt gattattata tgttcgtttg attggttaat
    19261 ctttatctcg atcgccgtac gcaagacacc gtgacgaaat ctattctatt tataatgtct
    19321 caatatttgt cccccaattc cgccaccgac gatggttcta attattccga aatcagattt
    19381 attgataaga actaggtctg caatatatct ttccttaacc acccctctct tttttaaccc
    19441 gaaaagctcc gccggcttca tggttatctt cttaatagcc tcttcaatca acatagaatt
    19501 attctgaacg gcaagcttca aatacctagg aaaagtgttc aacgaccttt cgtttttgac
    19561 aaaatcagac gaactacgtc ttgaatcgct atttgaagca atcaaaactc tttctcttgg
    19621 aagaagctta acaagataat caaaattaat atttcgatta gcgacgacgg ccttcagccc
    19681 tgttatgtcc atgactttca aaagcgccgc cttatgagaa aggccaagat tttcggctga
    19741 ttgcgctatt gtcttgccga ctaaataatc attttttgga gcatcaacta ttttaatatc
    19801 atttggatta aattccggca tatctctttc tatctttaac gccatttcgg aatttcttat
    19861 gttataaagc atagtttcca gatttctttc ctgagcccaa ataggcaaaa gcgtataaat
    19921 cgaatttaaa cttatatcaa atgggtaaac atagaaataa atgtttgctg aatctaaatt
    19981 cttatctatt aactccatag ccttatcaaa tttagattcg tacccattaa ggggtcttaa
    20041 atgactgata agaacttttg cccccgtttc tttggctata ttaattgttt cttcaacaga
    20101 atctaaaaga ttattcgtct catttcttaa atgagtggaa taaatagcgt cagactcttt
    20161 cacaacccca accagccttt tcagctcatc atttgaaata tgacgtccat gagcatatcc
    20221 caggccgctc gataaaccca tcgccccctc atagagcgcc ttatttaata ttcttttaaa
    20281 aacttctgtt tctttatcgg tcaaatccct ctcttc
//
LOCUS       DATDNZ010000019         6244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_581743, whole genome shotgun sequence.
ACCESSION   DATDNZ010000019 DATDNZ010000000
VERSION     DATDNZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581743"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..689
                     /gene="cyaB"
                     /locus_tag="VI432_00780"
     CDS             114..689
                     /gene="cyaB"
                     /locus_tag="VI432_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY4496668.1"
                     /translation="MNKKEIEIQVKLGDGKPLLEFLGKNADFKGEYHQIDEYYTPPHK
                     DFSASRPVNEWLRLRDSEGQYSINYKYWHYDGDEKSRYCDEYESRIEDIDTLRKIFKA
                     LDFRSVVTVDKYRKIWIYKHFEITMDDVKNLGDFVEIEYIGESTNLNPAEINKEMINF
                     LKDVGCKDIQRNYVGYAFSLMARDEVKWEEQ"
     gene            769..3309
                     /locus_tag="VI432_00785"
     CDS             769..3309
                     /locus_tag="VI432_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY4496669.1"
                     /translation="MVLEKNHIEQKAFWALSLDESFDLLKSSNKGIDMDDVPGRIKNF
                     GRNVIPRGKRLTRLDIFLNQFKSPLIIILLIAGIITVFLDHFVDSLFIFGIIIVNTLM
                     GFYQENKSETALSQLTTYIKERTRVIRGGVEKEVDAEEIVPGDLVRLISGSRIPADLR
                     LVKVKDLSIDESILTGESLPITNKIIDKVEYSSPVFERSSMAFAGTLVNGGLGQGLVV
                     ATGPNTELGKIAAMVEKGEKIKTPLQIAIGRFTLRLSLIIIFLTITLFSIGLMTGKDL
                     FDIFFLSVAVAVSAIPEGLPVALTVILAIGVERLAKKRGIVRQLLAAETLGGTTVILT
                     DKTGTLTQAKMKLSQIISTFPKEEVLEAALINTDIIIDNPDSPPGEWRLIGRPLEAAL
                     VAAAAEHNVFMSEYQNEIEILDQQPFNSNSKFSVFKVKKGKKTFSNYLGAPDILIDKS
                     NLNHQEKNNLLLTIDDLAFSGYRVLGVMRDRDFLGLLAFNDPVRPTVKESIEKTIKAG
                     VSTIILTGDHRGTAISVAKEVGLDVKDEDVVTGEDLKVMDDTTLKARLGNIKIFARVA
                     PEDKLRIARLYKERGDVVAMTGDGVNDSPALKEADIGIAVGSGTDVAKGAADLVILDD
                     NFDTIVAAIEEGRRTLSNIKKVIVYLLSNLLDEIILIGGAIIAGVALPLSALQILWVN
                     FFLDSFPAVSLAFENGKDHLKEKPTSLRGRLLDPEMRFLIIVVGIFTSVILFLMYLIL
                     LRIGLQEDLVRTFIFASFSLYSLFLIFSVRDLKTSIFEYNPFSNLYLTGAVMIGFILT
                     ASAIYIPFLQNILDTAPLNWFWVSGVFTFGILNILAVEFGKWLYRKSE"
     gene            3380..4747
                     /locus_tag="VI432_00790"
     CDS             3380..4747
                     /locus_tag="VI432_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4496670.1"
                     /translation="MSRAKYLILNTRYQRGFTLTEVIIYVGLFAGIATLLVGSLVTIL
                     NIEGRENSANEVTSQLSLAFQTVQRLVRESSLIEKVYEGTDEGASCVAFCSVKLRMED
                     SAKDPSIIRSDADGVYLKEGAGLEAAITTDRIRVDNFKFVKFETSGTKAILQIDAAFT
                     YNVGNPATAVSRALRSAVGRVSAATFDDDLLPNIDSSFNIGNSSNNWQSVFLSGDLTV
                     GGEAAPLASPFGRGTIYFDTGANKFKVSENNGNYVDLIQGGASYWTGETGKVYLVNSG
                     DNVGIGTTGPNQKLEVNGIIISGEDSATQGSLGFGVKYSGANIINTFGGQFSLGRTLI
                     GYGVKPKQGAAGYVSSAGNVAFARGGLVVSDSLIFLNATSGTVPIGTDITMTERFRVN
                     DDGNVGIGGSANPGSRLTVTGGDVYISNSTSGIILKSPDGTCARGTISNLDTLTFAST
                     ACPSN"
     gene            complement(4757..5449)
                     /locus_tag="VI432_00795"
     CDS             complement(4757..5449)
                     /locus_tag="VI432_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY4496671.1"
                     /translation="MRYFKKFKNIVLEILFPPTCISCKKLIHANENKALCAPCLEAID
                     INKVPIYIKPWLTISAVGSYDNPVLKELIHSFKYQGLMGSGNSLIDISSIYIEKALLG
                     SNLNIKNYIIIPIPLYNRKLKKRGFNQAEIIAKGISDKFNIEIWPNAIIKTKDTRSQT
                     ELKNNKERLKNIKNSFRLNPIFTERLKNHKTKNLILVDDVYTSGATMNEAAKALKRLG
                     AKNIIGFVIAKT"
     gene            5577..>6244
                     /gene="pilM"
                     /locus_tag="VI432_00800"
     CDS             5577..>6244
                     /gene="pilM"
                     /locus_tag="VI432_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY4496672.1"
                     /translation="MFNLFKSLTGGGGYLGVDIGTISIKIAELEKGLKKPVLKNYGTL
                     EGSGHLDRINDAIQTSSLKMMDKDVIGLLELLMKQIKVRSKKANVSIPSFSSFTTLLD
                     IPAMSPKEMTQAIYYQAKAFVPIPLQDVTIDWTVVGEYEDERGVKKQQIFLVSVPNEV
                     IKRYQRIFGAVGLSLQALEIEGFSTARVLSRKEQGDVLIVDIGGRSTTISVASKGFLK
                     FNAQT"
BASE COUNT         1872 a          996 c         1451 g         1925 t
ORIGIN      
        1 gtgagttcga atctcacctc ctccgcgata ggttgaaata gcaggattcg tccgggcatc
       61 cgtcaaagta gtctctgatt ttaaccaaaa gacactacca atttatatat tttatgaaca
      121 aaaaagaaat tgaaattcag gtgaagcttg gtgacggcaa gcctctttta gaatttttgg
      181 gcaagaacgc ggattttaaa ggagaatatc atcagattga tgaatattac acgccgccgc
      241 ataaggattt ctccgctagt cgtccggtga atgaatggtt gcgtcttagg gactctgaag
      301 gccaatactc gattaattat aaatattggc attatgacgg cgacgaaaaa agtcgctact
      361 gcgatgaata tgaaagcaga atagaagata ttgatactct acggaaaata tttaaggcgc
      421 ttgatttcag atcggttgtt accgttgata aatataggaa gatttggata tataagcatt
      481 ttgaaatcac aatggatgat gtaaaaaatc taggcgattt tgttgagatt gaatatatag
      541 gcgaatcaac caatcttaat ccggcagaga ttaataaaga aatgataaat tttttaaagg
      601 atgtcgggtg taaagatata caaagaaatt atgtcggtta tgcgttttcg ctgatggcga
      661 gagacgaagt taagtgggaa gagcaataat tgactacagc ttctagatta aacaatccct
      721 tcatactaga tactaaatac tttatactta atacctgatt actattttat ggtattagaa
      781 aaaaatcata ttgaacaaaa agccttttgg gcgttgtctt tggacgaatc ttttgatctt
      841 ttaaagagct cgaataaagg gattgatatg gacgatgttc ccgggcggat taagaatttc
      901 ggccgcaacg ttattcccag aggaaagaga ttaactcgcc tggatatttt tttaaatcag
      961 tttaaaagtc cgctgataat tattttattg attgcgggaa ttataactgt ttttctggat
     1021 catttcgtgg acagtttgtt tatatttgga ataatcatcg tcaatactct gatgggtttc
     1081 tatcaagaaa ataagtcaga gactgcattg tcacagctga caacttatat taaggaaaga
     1141 actagagtta ttaggggtgg agttgagaaa gaggttgatg cagaggaaat tgttcccggc
     1201 gatttggtgc gtcttatatc tggatctagg atccctgccg atttaaggct cgtgaaggtg
     1261 aaagatctat ctattgatga atcaatttta actggagaat ctctgcccat tacaaataaa
     1321 ataatagata aagttgaata ctcttctcct gtttttgaga ggagctctat ggcttttgct
     1381 ggaacgctgg tgaacggcgg actgggacaa ggtttggttg tagctacggg tccgaatact
     1441 gagctcggca agatcgccgc aatggttgag aaaggagaaa aaattaagac tcctttgcag
     1501 atagcaatcg gcagatttac attgcgtctc agtcttataa ttatatttct gactattacc
     1561 ctttttagta taggtcttat gaccggaaag gacctttttg acatattttt tctatcagtc
     1621 gcagtcgccg tgagcgcaat acccgaagga ttgccggtag ccttaacggt tattcttgct
     1681 ataggagtgg agagattagc taagaaacgg gggatagtaa gacagctatt agccgccgaa
     1741 actcttggcg gtacaacagt tattttgacg gacaaaacag gaacattaac gcaagccaag
     1801 atgaagctat ctcaaattat cagcaccttt cctaaagaag aagttttaga agcggcattg
     1861 ataaatacgg atattattat tgataatcct gatagcccgc ccggcgaatg gagactaata
     1921 gggaggcctc tcgaagcggc gcttgtggcc gccgccgccg agcacaacgt ttttatgagt
     1981 gaatatcaaa atgaaataga gattttagac caacagccat ttaattcaaa tagtaaattc
     2041 agcgttttta aggttaagaa aggcaagaaa acattttcaa attatcttgg cgcgcccgac
     2101 attttgatag ataaaagcaa tcttaatcat caagaaaaaa acaatttact tctcacaata
     2161 gacgatttgg cgttttccgg atatagggtt ttgggggtta tgagagacag agacttttta
     2221 ggattacttg ctttcaacga tccggtaagg ccgaccgtta aagagtcaat tgaaaaaact
     2281 attaaagccg gggtatcaac cattattctg acgggcgacc atagaggtac ggctatatcg
     2341 gtcgctaaag aagttggatt agatgttaaa gatgaggatg tggttactgg tgaagattta
     2401 aaagttatgg atgatactac gttaaaggca aggctcggta atataaaaat atttgcgagg
     2461 gttgctccag aagataagct tcgcatagca aggctttata aagaaagggg agatgttgtg
     2521 gcgatgacgg gtgacggcgt gaatgattcg cctgccctaa aagaggcgga cattgggata
     2581 gcggttggct ctggaacgga tgtggcgaaa ggggcggccg accttgttat tttagatgac
     2641 aattttgata ctatcgttgc ggccatagag gaaggcagga ggacattgtc taacattaag
     2701 aaagtcattg tttatctttt gtccaacctt ctagatgaga ttattttaat aggcggggct
     2761 attatagctg gggtggcgtt gccattgagc gctctgcaaa ttctttgggt taactttttt
     2821 ctggacagtt tccctgcggt ttctttagct tttgagaatg gcaaagatca tttgaaagag
     2881 aagccgacga gcctgagggg taggctttta gatccggaga tgagattttt gattatcgtt
     2941 gtgggcatct ttaccagcgt gattttgttt cttatgtatc tgatcctttt gagaattggc
     3001 cttcaagaag atttagtaag aacatttatt tttgcgtcat tttcccttta tagtctgttt
     3061 ttaatattct ctgttagaga tttaaaaact tccatatttg aatacaatcc gttttccaac
     3121 ctatatctta ccggagcggt aatgatagga tttattttaa ccgcctcagc catatatata
     3181 ccgttcctcc agaatatttt agacacagct ccacttaatt ggttctgggt ctcaggtgtt
     3241 tttacttttg gaatattgaa tatactggcg gttgaattcg ggaaatggct ttataggaag
     3301 agcgagtagg aaaaagtatg aagtattggg tattaagtat gtggtataat taaaaaataa
     3361 agacaatttt atataagcaa tgagtcgtgc taaatactta atactaaata ctagatacca
     3421 gagggggttt accctcaccg aagtaattat ctatgttggt cttttcgccg gaatagcgac
     3481 tcttttagtc ggatcgttgg ttactatttt gaatatagaa ggaagagaaa actcggcgaa
     3541 cgaagtcaca agtcagctaa gtttagcttt ccagacggtt cagcgattag ttagggaatc
     3601 cagtcttatt gaaaaagtct acgaaggaac ggacgagggt gcgagttgtg tcgctttctg
     3661 ttcggttaaa ttaagaatgg aagattcggc taaagacccg tcaattataa gatctgatgc
     3721 tgatggagtt tatttaaaag aaggagctgg attggaggcg gcgattacta cggataggat
     3781 aagggttgat aattttaaat ttgttaaatt tgagacttct ggcacgaaag cgattttaca
     3841 gattgatgcg gcttttacat ataatgttgg aaatccggct acggcggttt caagggcctt
     3901 gcgttctgct gtcggcagag tgagcgccgc gacttttgat gatgatcttc tgccgaatat
     3961 tgacagcagt tttaatatag gcaattcgtc aaacaactgg caatccgttt ttttgtctgg
     4021 agacttgacc gttggaggag aggctgctcc tcttgcttct ccatttggca gaggaacaat
     4081 ttattttgac acaggcgcga ataaatttaa ggtgtcagag aataatggaa attatgttga
     4141 tttgattcaa ggaggagcaa gctattggac tggcgaaacg ggcaaggttt atcttgtaaa
     4201 tagtggagat aacgttggta taggaacaac cggtccaaac cagaaattag aagtgaatgg
     4261 tattataata tctggcgaag attcggctac tcagggatct ctgggttttg gagtcaaata
     4321 ttcaggagca aatataatta atactttcgg cggacagttt tcattgggaa ggactttgat
     4381 tggttatggc gttaaaccaa aacagggggc ggctggatat gtttcttctg cagggaatgt
     4441 cgctttcgca agaggtggct tagtggtatc ggacagctta atttttctaa acgcaacgtc
     4501 tggaacagtt ccaattggga cggatattac tatgacagaa cgttttcgag tcaatgatga
     4561 cggcaacgtt gggattggtg gtagcgcgaa tcccggttcc aggttaaccg taaccggtgg
     4621 cgatgtatac atcagtaaca gcaccagtgg aataattcta aaatctccag atggaacttg
     4681 cgcaagagga acaatcagca atctagacac gctaactttt gcatcaactg cctgcccgtc
     4741 taattaatgt ccagatttag gtttttgcaa tcacaaaccc aataatattt tttgccccga
     4801 gacgttttag cgctttagcg gcctcattca tcgttgcgcc ggaagtataa acgtcgtcaa
     4861 cgagaattag gtttttagtt ttatggtttt ttagtctctc agtaaaaata ggattcaaac
     4921 ggaagctatt tttaatgttc ttcaatcgct ctttgttatt ttttagttct gtctgcgatc
     4981 tggtgtcttt tgttttgatt atggcatttg gccagatttc tatattgaat ttgtcggata
     5041 ttcctttggc tattatttca gcttgattga aacctctttt tttaagcttc ctattatata
     5101 gagggattgg gataataata taatttttga tatttagatt ggatccgaga agagctttct
     5161 ctatgtatat agaggatata tctataagcg agttgccgga tcccattagg ccttgatatt
     5221 tgaaagagtg aataagctcc ttcaaaacag gattgtcata agaaccgaca gcagagatgg
     5281 tgagccatgg ttttatgtag atagggacct tgtttatatc tatagcctca aggcatggtg
     5341 cgcacagggc tttattttcg tttgcgtgta taagtttttt acatgaaatg caagttggtg
     5401 gaaacagtat ttccaaaaca atgtttttaa attttttgaa gtatctcata tgtattagaa
     5461 attatacttt aaagacggtg atttattgcg cgtatattaa ttataattag tttaagctgc
     5521 gaattatcaa atatttctaa cgggacaggt gtatgataag attataactt aatagcatgt
     5581 ttaacttatt taaatcattg actggaggcg gtggatatct gggagttgat ataggcacaa
     5641 tctccataaa aatagcagaa ctagaaaaag gcttgaaaaa gcctgttttg aaaaattacg
     5701 ggacgcttga gggatctgga catctagaca gaataaatga tgcgatacaa acgagcagtt
     5761 tgaagatgat ggacaaggat gttatcggcc ttttagaact cttgatgaag cagattaaag
     5821 ttagaagcaa aaaagcaaac gtttcaatcc cttcattttc ttcttttaca actcttttag
     5881 atataccggc aatgtctcct aaagaaatga cacaggctat ttattatcag gccaaagctt
     5941 ttgttcctat tccgcttcag gacgttacaa ttgattggac tgttgtcgga gaatacgaag
     6001 acgagagagg cgttaaaaaa caacagatat ttttagtttc agttcctaat gaagtaatca
     6061 aaagatatca gaggattttc ggcgccgtcg gtttaagttt gcaagccctt gagatagagg
     6121 gtttcagcac ggcaagggtt ttgagcagaa aagagcaggg agatgtttta atagttgata
     6181 tagggggcag gtctactacg atctcggttg cttcaaaggg atttttaaaa tttaacgcgc
     6241 aaac
//
LOCUS       DATDNZ010000020         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1888450, whole genome shotgun sequence.
ACCESSION   DATDNZ010000020 DATDNZ010000000
VERSION     DATDNZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888450"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VI432_00805"
     CDS             complement(<1..1080)
                     /locus_tag="VI432_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496673.1"
                     /translation="MNTVFNLNQSVAVEAVNFRRIFPRALHENITRVFGALTIFFLVT
                     SIILPRLGNVLTSQDHLFLALFFWSISFFGISIFSYGFLQQGFVSHNKRTLHAALEAS
                     KLGEVVNFAAYLDFYAARTVLATQHKGSNYSIRSLTDNLLREKRMKWVCIRLNLNVDS
                     LRKSLAAIPAQPQSGQNDFLHAIELAANHAVNRHHGTIGILDLFSASAVADYAFKRLL
                     FDQGIEPRDFDAIVHWQDRIYHVLDHRRRFWLKENLFRSQPIGKSWAAGFTALLDKYG
                     RDITADMQKGGLDLRIIGRKREIDNIEQVLAKAGENNVLIVGEPGVGRETVLYGFAKR
                     LLEGRSLPQLNRQRLVKLDLSATIAG"
     gene            complement(1084..1461)
                     /locus_tag="VI432_00810"
     CDS             complement(1084..1461)
                     /locus_tag="VI432_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496674.1"
                     /translation="MFFRYFVWHYGPGLRSFLAAERNYIDFGWYFFSISELTRSLFAP
                     WHRIAEKKPGNIVTPEYWQALWGNIISRVLGSIVRLMTIFIGLFFEAVILLIITILTF
                     IWITAPIIIPIGFFLGFAELSNF"
     gene            complement(1936..2313)
                     /locus_tag="VI432_00815"
     CDS             complement(1936..2313)
                     /locus_tag="VI432_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496675.1"
                     /translation="MLSAEILFIEDNKEMSNVVTAQLEKQGVEISVASDGLKGLELAE
                     KNKPDLILLDILLPGISGFEVLKRLKKNPDISNIPVVMLSVLWEADQVAKALSLGAAD
                     FITKSNIPASAIFERIKKYLPQK"
     gene            complement(2347..>2782)
                     /locus_tag="VI432_00820"
     CDS             complement(2347..>2782)
                     /locus_tag="VI432_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4496676.1"
                     /translation="SFKADAAKKNVIIVFDKISMPAEAISLDVGRIRSVLNILIRNAL
                     NYTPSGGQIVIKLKKEIENDKEVFHLIIQDNGVGIRREDLNKIFSRFYRSPDVIKIIP
                     DGAGLGLFIAKHIIEAHNGRLWAESDGIGKGSSFHVILPKTI"
BASE COUNT          899 a          567 c          509 g          807 t
ORIGIN      
        1 tccggcgatt gttgcagaaa gatcgagctt aactagtctt tgacggttaa gttgcggtaa
       61 agaacggcct tcaagaagtc gttttgcaaa accgtataaa actgtttctc tgccgactcc
      121 tggctctcct actattaaaa cattattctc gccagcctta gccaaaacct gttcaatatt
      181 atcaatttct cttttacgac cgattatcct taaatctaaa ccgcctttct gcatatcagc
      241 agttatatcg cggccatatt tatcaagaag cgcggtaaag cccgcggccc aacttttccc
      301 tataggttgc gatctaaata aattttcctt aagccaaaaa cgacgcctgt gatctaaaac
      361 gtgataaatt ctgtcttgcc aatgcactat agcatcaaaa tctcttggtt ctataccttg
      421 atcaaaaagg aggcgtttga atgcataatc ggctacagca gacgctgaaa acagatccaa
      481 aataccaata gttccgtggt ggcgattgac cgcatgatta gccgccagct caattgcgtg
      541 taaaaaatca ttttgaccgc tttgtggttg tgcaggtatg gcggctaaag acttacgtaa
      601 agaatcgaca ttaagattca atcgtatgca aacccatttc attctttttt cgcgtagaag
      661 attatctgta agcgaacgta tggaataatt gctccctttg tgttgcgtag ccaaaacggt
      721 ccgcgccgca taaaaatcaa gataggcggc aaaattaact acctccccta attttgaagc
      781 ttctaatgcg gcatgtagag ttcttttgtt atgactgaca aatccttgtt gtaaaaatcc
      841 ataagaaaat atggatatgc caaaaaaaga tatgctccag aagaatagcg caaggaacaa
      901 atgatcctgc gaggtcaaaa cgttaccaag tcgaggcaat attatactcg tcacaagaaa
      961 aaatattgtg agggcgccaa aaacacgagt tatgttctca tgcaaagctc gcgggaatat
     1021 tcttctaaaa ttcacggctt ccacggcaac ggattgatta agattaaaaa cagtattcat
     1081 gacttaaaaa ttagatagct ccgcaaaacc cagaaaaaat ccgataggta taataattgg
     1141 ggctgttatc catataaaag ttaaaatagt tataatcaaa agaataaccg cttcaaaaaa
     1201 cagaccgatg aaaatcgtca tcaagcgcac tatactgccc aaaactctag aaatgatatt
     1261 gccccacagg gcttgccaat actcgggcgt cacaatattg ccaggttttt tctctgctat
     1321 cctgtgccaa ggagcaaaaa gcgaacgggt cagttcgctg atggaaaaaa agtaccagcc
     1381 aaaatcaata taattgcgct cggctgctaa aaacgagcgc agacctgggc cataatgcca
     1441 aacgaaataa cgaaaaaaca tacgtttata ttatagcaaa taaataagaa agagggcgga
     1501 gtttgtggtt aaaggtcatt gactttataa atttatcaat cgtataattg aagcaatacg
     1561 aacattaaaa tctatatgcg tcgggaggat tccgcgatgg acaaattgcg cgaattttgc
     1621 ccggcgtgcc gcggcaaatt attgatatca ttttttgatg ccaatttccg taaaaaagac
     1681 gttaacgacc aacttatctt tgacatgcct gcgagttttt gtaagcactg cgaccaacta
     1741 tacttagaag agaatcttgt acggattttg gggcttgaag gatatatctg cgtgtttgca
     1801 atacagaggg ataagcaatt ctaccctgat tggaaagatt ttctaaaata acccccttaa
     1861 attaaggggg tattatttta aagatagatg acattttaag caataggttt aaaacgattt
     1921 ttaattgcgt atctattatt tttggggaag atatttttta atccgttcaa agatcgccga
     1981 ggctggtata ttagattttg ttataaaatc agccgcgcct aaactcaacg ccttagcaac
     2041 ttggtctgct tcccataata cagacaacat aacaacgggt atatttgaaa tatccggatt
     2101 ttttttaagt cgcttcaaaa cctcaaaacc ggaaattccc ggaagaagta tatcaagcaa
     2161 tataagatct ggcttatttt tttcggctag ctccaagccc tttaagccgt ctgaagccac
     2221 ggatatctcg acaccttgtt tttctaactg cgccgttaca acattggaca tctctttatt
     2281 atcctcgatg aatagaattt ctgctgatag cataattata taattagata tttttatatt
     2341 ttaaaatcaa attgttttag gtaaaataac atgaaaagaa ctgcctttcc ctataccatc
     2401 ggattcggcc cataacctcc cattatgggc ttcaattatg tgtttagcta taaaaagacc
     2461 cagtcccgcg ccatcaggaa taattttaat tacatctggc gagcgataaa aacgactgaa
     2521 gattttatta agatcttccc tgcgaatacc cacgccgtta tcctggatta tcaagtggaa
     2581 aacctcctta tcattttcaa tttctttctt gagtttaatc actatctgac cgccagacgg
     2641 cgtataattc aaggcattac gaattaaaat attcaatacg cttcgtattc ttccaacatc
     2701 aagcgatatt gcttcggctg gcatagaaat tttatcaaaa acgataataa cattcttttt
     2761 agccgcatcg gctttaaacg at
//
LOCUS       DATDNZ010000021         4240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_327896, whole genome shotgun sequence.
ACCESSION   DATDNZ010000021 DATDNZ010000000
VERSION     DATDNZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327896"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VI432_00825"
     CDS             complement(<1..1423)
                     /locus_tag="VI432_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496677.1"
                     /translation="MRKAIVFILLISVLWTVNVPVVEAASIAPGVSASAGNTTTEFET
                     DYTVKGKLGTAPLLPNSTIEIIFPSSFSFGSMFVDHPGLTGTWSTTGTTVKFKTSQEI
                     LGGTNLTFEILGVINSNTPGTYTIEMRSTLDAPGTPGEFSNPVTIEDAASGGGFGELE
                     DVLTCSGVVSCIKKVVKAIVNLPKFLINLLVIILQTIISLLITFMIWIGASLIQFAIN
                     TNFQLVNHINDIPPTEAAKIGFDITLSLTNIGFVIAIIFIAFATILKMQNFAIRQALP
                     RLIIIAILINFSMFFATAIINISNLLGGGLTSIVIGGSPGSVPDYVGIFSQFFNSSYT
                     AVQIGNLSSIPGVPGWLSTLLDGGLNFLEAAISGFVSIISQVLFGLLVFFTLIATAIM
                     LVIRYIALSFLIMLLPLALLTNIFPGLQIPGGGSPWKKWTENFTKWVIFFPAVMLFTA
                     IALEVAGKLNNLGPYGTFQSLTNV"
     gene            complement(1450..2358)
                     /gene="rsmA"
                     /locus_tag="VI432_00830"
     CDS             complement(1450..2358)
                     /gene="rsmA"
                     /locus_tag="VI432_00830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY4496678.1"
                     /translation="MQTKLKRRQKTIKADYQYAKKSLGQNFLTDISVLKKIVRAVDIK
                     KGDVVVEIGPGHGELTKYLLEAKPTRLILIEKDGRLIGGLHKKFQNTNHKKQTSNKTQ
                     IPNIEIIDGDVLDIIENWKLKIGNSTSYKLVGNIPYYITGHLLRLIGEQITSGKSQIT
                     KIVLTVQKEVAERICAKPPKMNILAASVQFWGKPEIISIIPREKFNPAPKIDSAIIKI
                     VPVKSRWPNIDGQNFYKLIKVLFKQPRKTIWNNLKQLTLRQAQGKQFTEERLKKLLIQ
                     LEINPEDRPQNLSIDKITDLNSVIWQ"
     gene            2398..3435
                     /locus_tag="VI432_00835"
     CDS             2398..3435
                     /locus_tag="VI432_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY4496679.1"
                     /translation="MLPKLLFRFKVRPDALHRWWGFVPVLSVSSALVFTVINTIANPA
                     RPSTIADYSAFGDPLLNQPLLNIQDSAFLNNTAPSDNSFLDSDSFVTPIIKEGSFQNT
                     NTSPSNNIKFIGRGLLTYKIVEGDTLSTIAANNGISVNTILWANNLKRSATIRPGQEV
                     VILPVSGILHEVRDGETPESISSLYAISLKDFTIYNKTRISAGDLVVVPDAKPILSSS
                     STEGRVDARNYLAFPVKEAVNFGLLHLNAVDISAPCGTPIYASAEGLVTAVGNPKNWN
                     DGWGGYIRIEHPNGIETFYSHNSKNLVSVDDYVSRGDEIAKIGNTGKVHGPTGCHVHF
                     GVFGAKHPFAK"
     gene            complement(3526..>4240)
                     /locus_tag="VI432_00840"
     CDS             complement(3526..>4240)
                     /locus_tag="VI432_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4496680.1"
                     /translation="PHQTASPVSIVGGGQNPRPGEISLAHRGVLFLDELPEFRRDLLE
                     ALRQPLESGKIIISRVKNNLIFPARFILVAAMNPCPCGYYGDKEEIGGKECKCSAYEI
                     FRYQKKISGPLLDRIDIQINVPRTKIENLKKPREDSSNIKSSILSARAKQIKRFENMD
                     RKILTNAEMNSKECEELINLSANAEKFLEKIFDTSNLSTRTYYRILKTARTIADLEES
                     ETIETPHISEAFQLRVRNE"
BASE COUNT         1187 a          812 c          807 g         1434 t
ORIGIN      
        1 atacattggt caagctttgg aacgtgccat atggaccaag attatttaat ttaccagcta
       61 cttccagcgc tatagcagtg aagagcatca ccgcagggaa gaatataacc cacttcgtga
      121 aattctctgt ccatttcttc caaggactac cccctccagg tatctgaaga ccgggaaaaa
      181 tattggttag gagagctaat ggcagaagca taattaagaa actcaatgct atatatctta
      241 tcaccaacat aatcgctgtt gcgattagtg taaaaaacac caacaaacca aataatactt
      301 gtgagataat actgacaaat ccagagatag cagcctccag gaagttgagc cccccatcta
      361 ataatgtgga tagccatcct ggcacacctg gaatcgaaga taagttaccg atttgtactg
      421 ctgtataaga tgaattaaaa aattggctaa aaattccaac atagtctggc accgaaccag
      481 gactaccccc tataactatt gatgttaatc ctcctcccaa taaattactt atattaatta
      541 tcgcagttgc aaaaaacata ctgaaattta taagaatagc gataattatt aatcttggca
      601 gagcttgtcg tatggcaaaa ttttgcattt ttaatattgt tgcaaaagct ataaaaataa
      661 ttgcgattac aaagcctata ttagttaacg ataaagttat atcaaatcct atttttgcgg
      721 cttcggttgg gggaatgtcg ttgatatgat ttactaattg gaagttggtg tttatggcaa
      781 attgaattag gcttgcacca atccaaatca taaaggttat caacagactg attatcgttt
      841 gaagaattat tactaagaga ttaattagaa atttaggaag gttaacgata gctttcacaa
      901 cttttttaat acagctaacc actccactac aggtcaatac atcttctaat tctccgaaac
      961 cgccgccaga ggccgcatcc tctatggtaa ccggattaga aaattctcct ggcgtgccgg
     1021 gcgcatcaag cgtggaccgc atttctatag tgtaagtacc cggcgtgttg ctgtttatta
     1081 cacctaatat ttcaaaagtt aaatttgttc cacctaatat ttcttgactg gttttgaatt
     1141 taactgttgt gcctgtcgtt gaccacgtgc cagtgagtcc tggatgatct acgaacatac
     1201 tgccgaagga aaaactggaa gggaaaatta tttctatcgt actattgggg agaagaggcg
     1261 ccgtgcccaa tttgcctttt actgtgtaat cagtttcaaa ttctgttgtt gtattaccgg
     1321 ccgaggctga tacgcctggg gctatggagg ctgcttcaac tactggtaca ttaactgtcc
     1381 acaatacaga tattaataat ataaaaacta tagctttgcg cattaggatt tatctttaga
     1441 atatcacgac tattgccaaa ttacactatt taggtcggtt attttatcta tacttaaatt
     1501 ttgcggccga tcttcgggat taatctcaag ttgaattagt aattttttaa gcctttcctc
     1561 ggtaaattgt ttaccctgag cttgtcgaag ggttaattgt tttaaattat tccatatcgt
     1621 ttttctcggt tgtttaaata aaactttaat aagcttataa aagttttggc catcaatatt
     1681 aggccaccga cttttgactg gaacaatttt gattattgcg gaatctatct taggcgctgg
     1741 gttgaacttt tcgcggggaa ttatgcttat aatttcaggt ttgccccaga attgaacgct
     1801 tgctgctaga atattcattt ttggcggttt tgcgcatatt ctttctgcta cttctttttg
     1861 tacggtcaat acaatttttg ttatttgcga tttgccactt gttatttgtt cacctataag
     1921 ccgaagtaaa tgcccggtta tgtagtaggg aatattaccg accaatttat aactggtgga
     1981 atttccaatt ttcaatttcc aattttcaat aatatccaaa acatccccat caataatttc
     2041 tatatttggg atttgtgttt tgttacttgt ttgttttttg tgatttgtat tttgaaattt
     2101 tttatgtagg ccaccaatta gccgcccatc cttttctata aggattaggc gcgttggttt
     2161 agcttccaat agatatttcg tcagctctcc atggcccgga ccgatttcca ccacgacatc
     2221 acccttttta atatctacag ccctgacaat tttctttaaa acagaaatgt cggttagaaa
     2281 attctgcccc aaagattttt tggcatattg ataatcggct tttatggttt tctgtctcct
     2341 cttaagtttt gtttgcattt ttatctggtt gttttaatat ggctactatc attgcaaatg
     2401 ctaccaaaat tgctatttag gtttaaagtc aggcctgacg cgcttcatag atggtggggg
     2461 tttgtaccgg ttttatcggt ttcctccgcg cttgtattta cagtaataaa tacaatcgcg
     2521 aatcctgccc gcccctcaac tatagccgat tatagcgctt ttggtgaccc actgcttaat
     2581 cagccccttt taaacattca ggatagcgca tttttgaata ataccgctcc atctgataat
     2641 agttttttag acagcgatag ttttgtaacg cctattatca aagaggggtc ttttcaaaat
     2701 acgaacacaa gcccctctaa taatattaaa tttataggtc gcggattgct gacttataaa
     2761 atagtcgaag gagatacatt atcgaccatc gcggctaata atggcatatc ggttaatacc
     2821 attctttggg cgaataatct taaaagatcc gctacgataa ggcctggaca ggaagttgta
     2881 atattgcctg tttcgggcat tctgcatgag gtgagagacg gagagacgcc cgaatcaata
     2941 tcttctttat atgcgatatc attaaaagat ttcacgattt ataacaaaac aagaataagc
     3001 gcaggagatt tagttgtggt gcctgacgct aaaccaatac tcagttcaag ttcaacagag
     3061 ggcagggttg atgctaggaa ttatttagcc tttccggtta aggaggctgt taattttggt
     3121 ctacttcatc taaacgctgt tgatatttcc gctccttgcg gaacgccaat atatgcgtct
     3181 gccgaaggtt tggtgacggc ggttggcaat cctaaaaatt ggaatgatgg ttggggagga
     3241 tatattcgta ttgaacatcc caacggcata gaaacttttt attctcataa ttctaagaat
     3301 cttgtgtcgg ttgatgatta tgtaagtcgt ggagacgaga tagccaaaat cggcaatacc
     3361 ggaaaggtgc atggcccaac aggatgtcat gtccatttcg gagtctttgg cgctaagcat
     3421 ccattcgcaa agtaagtgaa aatttaaata tcaaaatata aaatttttgt tgtccgctat
     3481 tctgcgggtt ccattagtct tgggttttta aatttacaat ttaatttact catttcttac
     3541 tctcaattga aaagcttcgc ttatatgagg agtttcaatt gtttcgcttt cctccaaatc
     3601 ggcaatggtt cgggctgttt taagtattct ataataagtt cttgttgaaa ggttagaggt
     3661 gtcaaaaatc ttttctaaga atttttcggc gttggctgaa agattgatta gttcttcgca
     3721 ttcttttgaa ttcatttcag cgtttgtgag tatttttcta tccatattct caaatctttt
     3781 aatttgtttt gctcttgccg acaaaatgct cgatttgata ttgcttgagt cttctcttgg
     3841 tttcttaaga ttctcaattt tggttcttgg cacattaatt tgaatatcaa ttctgtctaa
     3901 aagcggccca gaaatctttt tctgatatct gaatatttca taagcgctgc atttgcattc
     3961 ttttccgcct atttcttctt tatctccata atatccgcat ggacacgggt tcatcgcggc
     4021 aactaatata aatcttgctg gaaaaataag attatttttt acccttgaga taattatttt
     4081 ccccgattcc aagggctgtc ttagggcttc aagcaggtcc cgcctgaatt ctggcagttc
     4141 atccaggaac aagacgcctc tatgcgccaa acttatttct ccaggtcttg ggttttggcc
     4201 tccgcccacg attgaaaccg gagaagctgt ttgatgtggc
//
LOCUS       DATDNZ010000022        11140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3215258, whole genome shotgun sequence.
ACCESSION   DATDNZ010000022 DATDNZ010000000
VERSION     DATDNZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215258"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VI432_00845"
     CDS             <1..1050
                     /locus_tag="VI432_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HEY4496681.1"
                     /translation="ITLYQNINYGGTSQTFTGGFYPNFVDLGINDWASSIKFGPAPPP
                     PPPSNYNLNVNSSGYSGISVSSSTGHSGTTNYTKSVSSGANVNLRSLIGVINVQGGNN
                     HYFTSWSGSSACLDTNYEITFSMPASNTTCTTNFVSTCGNLSLYNSNNLGGGCEGYSD
                     SWSFINGGKNSGATKNNFTAFVFPNANFSGGYWKITQNSTLRFPSNSIYSAIVSGSGP
                     DEVRVTTGTDNGAGTDDRVYLKVYNVETGWRYIHLDKNGDDFENGNVDTYTTSNNTNG
                     DPVVIGPGDTHFRMYKSPDGTSFGDWKLQHWSATDNGQVIINDSHNGWLNDQTTQTPE
                     RVRCYHSIGGPGTTC"
     gene            1061..1276
                     /locus_tag="VI432_00850"
     CDS             1061..1276
                     /locus_tag="VI432_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496682.1"
                     /translation="MKRNISIIIIIAAFLLGLAYFVFTRRSLDNILLVPPELLNPSTT
                     DEPPLIGGKEDGAKGQTEPDPPPEDQK"
     gene            1347..1673
                     /locus_tag="VI432_00855"
     CDS             1347..1673
                     /locus_tag="VI432_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496683.1"
                     /translation="MLSIAFYLYRPYKTFEIVVKNNEIQSLQVGMDESSVPRHLYVNK
                     GDWVIIKITVDQDSRLYISGYDKDIDIETDKEAKIYFKADDVGVFQLELSGSSADLGV
                     IEVLSR"
     gene            1751..2044
                     /locus_tag="VI432_00860"
     CDS             1751..2044
                     /locus_tag="VI432_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY4496684.1"
                     /translation="MTIKPLSNHIFLESQEEEKITKSGIVIPDTVEKERPIQGRVFAV
                     GPGKLDNNGRLMPISVKVGDLVLFKKYGLDEIEIENKKYLVGDEDDILAIIGQ"
     gene            2184..3860
                     /gene="groL"
                     /locus_tag="VI432_00865"
     CDS             2184..3860
                     /gene="groL"
                     /locus_tag="VI432_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722916.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY4496685.1"
                     /translation="MAKNILFNENARNALKKGVNKLAEAVKMTLGPRGRAAVIEKSYG
                     APQVTFDGVTVAKEIELADRFENLGAELIKQAADKTNDNVGDGTTTSVVLAHSMMTEG
                     DKIVEQKGINVIQIAKELERAGEKVVKMLDEQKELINDNKKIEEVASLSAKDETIGKL
                     IAEVMSKIGKEGVVTVEDSNTVANSYEIVEGMQFDRGYISPYMMTNAERMESAIEDPY
                     ILVTDKKISSVKELLPILEKLMNSGKKDLVIIADDIDGEALATLVVNKLRGVFNVLAV
                     KAPGFGDRRKEMLEDIAIVTGAKFISEQTAEKLENTELDSLGRAHRVVSNKDNTTIVD
                     GKGVKEEIENRISQIKAQLKKTDSDFDKDKLKERLGKLTGGVAVIKVGAPTESAQKEL
                     KQRVEDAVSATRAAMEGGIVPGGGVALFNAYLAMQNEIGEIKDDVVEGAYSILEKALV
                     SPIEAIAINSGESASAIKDKLMNMKKKSTSQDKNWIGFDAFSGEIVDDLRQEGIIDPL
                     KVTREAFLNAVSVASNYLTIGAAVTDIPEKDKPEMGGLPGQGGMPGMGGF"
     gene            3898..4446
                     /locus_tag="VI432_00870"
     CDS             3898..4446
                     /locus_tag="VI432_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY4496686.1"
                     /translation="MDALYIFLAIATVWVIWTYNRFVTLRYRAKEALSDIDVQTKRRY
                     DLIPNLVDTVKGYMTHEKSVLENVTKARADVVGMSGDQIDKAEAENMLSSTLKTLFAV
                     AENYPDLKANANFLDLQRELADTENKIQAARRFYNTTVRDLNIKIDIFPSNLIANLFR
                     FNKEKFFETEVAAEKEPVKVSF"
     gene            4526..5425
                     /locus_tag="VI432_00875"
     CDS             4526..5425
                     /locus_tag="VI432_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY4496687.1"
                     /translation="MASLYTHKESNIRKTFFLFFIFLVVVIGIGWAFSYVYNSSLILW
                     IAVIFSSVMAIISYWYSDKIVLRMANAVPIDMKNNPELYRIVENLSITAGLPMPRLYL
                     VREQAPNAFATGRNPQNSVVAVTEGLLNKLDRSELEGVIAHELSHIGNRDMLVGTVAV
                     ILVGFISLIADMFMRSLFWGGMRSRDRGNGHAVIIFIGIVLSILAPIAAMLMQLAISR
                     KRELLADASGSLLTRYPEGLASALIKISTDFTPMRAANNTTAHLWLDDPFKGKRKTSF
                     LHKLFMTHPPVEDRVRALRGIQV"
     gene            complement(5464..5739)
                     /locus_tag="VI432_00880"
     CDS             complement(5464..5739)
                     /locus_tag="VI432_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496688.1"
                     /translation="MNAEKLLEATALYTRKIEEMNIPAKSFKRNELRPSNRDALAHCH
                     RMLQKIPQLIKEGRVEKVHRWLGFIQACLWMSGTYSIQRMKDHLKPD"
     gene            5955..6041
                     /locus_tag="VI432_00885"
     tRNA            5955..6041
                     /locus_tag="VI432_00885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5989..5991,aa:Ser,seq:gga)
     gene            6187..6441
                     /locus_tag="VI432_00890"
     CDS             6187..6441
                     /locus_tag="VI432_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005260917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY4496689.1"
                     /translation="MTKKITKGALHKVPSDLREAFFYSPKALAAWQDITLLARNEWIC
                     WAISVKKDETRRDHIQRTISELKEGKRRPCCWAGCPHRAK"
     gene            complement(6671..7198)
                     /locus_tag="VI432_00895"
     CDS             complement(6671..7198)
                     /locus_tag="VI432_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY4496690.1"
                     /translation="MINLEEEEKILLEQRRHWFVIGTTGAIMFGVILAPIIFVIVILG
                     AFPEITAFFEDIKFIWIAVFFILSWTLIMWVSFFIAWTDYYLDVLIVTNKRVVDVEQR
                     GLFRRDLATVPLQNIQDIKIEINGLIPTLLRFGNLHIQTAGVSKEVLIKDIKEPTEVK
                     KIILSAYHDTLGKQL"
     gene            7512..8456
                     /locus_tag="VI432_00900"
     CDS             7512..8456
                     /locus_tag="VI432_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEY4496691.1"
                     /translation="MLFFWIIVFAVSMAVLVKGADWLLIGAEKVGIALGIPPFIIGVT
                     IVAAGTSFPELVSSIAAVIKGAEEVVAANVIGSNIANILLVVGLSAVFAGRLTVTKNL
                     IDLDLPLLILSTTLFLGVVWDGLITVGESIILVSTYLIYAVYTAVHRDDEEVLSKESS
                     PKDKPKLKINDFVFIAVGIAGLVVGSKYLIDSVISLSEILNVGKGIIAITAVAIGTSL
                     PELLVSVKAAIHKKSEVALGNIFGSNVFNVLVVAGIPGLLGNLPLDEKTFLLGVPALA
                     VTTLIFVISGISKRIYIWEGSFFLAIYALFLGKLFNLF"
     gene            complement(8453..9256)
                     /locus_tag="VI432_00905"
     CDS             complement(8453..9256)
                     /locus_tag="VI432_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496692.1"
                     /translation="MNHKLIPKIPISHIFEVLGKTPAGESLKNTVRFNVYRDSESIGL
                     WKMALGPSAITYSHMIYMHALVNYLLHRELTSNPGLFTADEHRELLLAAACHDFGEAI
                     INGEGIGDVSDPYKNEELEEKEADMFQRVLFSLDLDSELKKEMFNSYQSVIRGGNKKM
                     YDYFRGVEQMEYIMTGSNIFRQKENIKIKNGAILIGSVLAVSIKPTIEKAKKFLSIRE
                     ILSKNKKAITGMFKHSKEKYTKIRNPMLKKPGAAISPALKSWQQYRKSL"
     gene            9295..10569
                     /locus_tag="VI432_00910"
     CDS             9295..10569
                     /locus_tag="VI432_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HEY4496693.1"
                     /translation="MKIVADLHIHSPYSRAVSKNMTLQNLDYFARLKGINVMGTGDFT
                     HPMWIDEIQEKLEPAEAGLYILKDEYLYPESKGEKTRFMLTVEISSIYSKGGRARRIH
                     NLIFTPTIEAALKINAELGWRGNIKSDGRPILGMDPKEIAKIIFNIEPRSAIIPAHAW
                     TPWFSIFGSMSGFDSIEECFDEFAPQIFAIETGLSSDPAMNWRLSKLDNIALISNSDS
                     HSLKKIGREANVFDTQLSYDGIIEAIKSHDPKKFLFTVEFFPEEGKYHYDGHRVCKYS
                     CDPDKTNDHNNLCPRCGKKMTIGVLNRVRQLADRESGFASKNAIPFKNLIPLDEIIAD
                     VIGVGTASKKVNAEYMNLINKIGTEFSALLEASESELASASSPRIASAITMVREGKVR
                     IEPGYDGEYGKIGIMPKEGSEEKQEGLFLTGL"
     gene            complement(10613..>11140)
                     /locus_tag="VI432_00915"
     CDS             complement(10613..>11140)
                     /locus_tag="VI432_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496694.1"
                     /translation="LMIVLSIKMPSSSNFDLIMEEIDIAVSGQKNKKYIINIPKRKGV
                     ITNIMIRLSYIAAFFFSLYAISKILLSINFSLANIVVFIFFTSLVSAAGVRVSRQAKE
                     ISLEKKKATLSSFLIDLFAIPFITIGKWGLMALGKLNILVVVFNLIIDLPLQIFIEFL
                     ENFRGFIKSRKDDIS"
BASE COUNT         3349 a         1955 c         2415 g         3421 t
ORIGIN      
        1 ataactcttt atcaaaatat aaactatggc ggaacatcac aaacttttac tggcggtttc
       61 tatccgaatt ttgttgattt gggtattaac gattgggcct catctataaa atttggcccc
      121 gcgccacctc ctccgcctcc atcaaactat aatctcaatg ttaattcttc cggttattct
      181 ggcatttcgg tttcgagctc aaccggacat agcggcacta ctaattatac gaagagcgta
      241 tcttctggtg cgaatgttaa tcttagatca ttaatcggcg tcattaacgt tcaagggggt
      301 aataatcatt atttcacaag ttggtctggc tcatcagcct gcttagatac aaattatgaa
      361 attacttttt cgatgcctgc gtcgaataca acctgcacta ccaattttgt ttccacttgt
      421 ggaaatcttt cgttgtataa ttctaataat ttaggcggag gttgcgaagg atattccgat
      481 agctggtcat ttattaatgg tggcaagaat tctggagcaa ctaaaaataa cttcacagct
      541 tttgtattcc cgaatgcaaa tttcagtgga ggatattgga aaattacaca gaattctaca
      601 cttcgttttc catccaactc tatatattca gcaattgtta gcggttcagg ccctgatgag
      661 gtgcgtgtaa ctaccggtac tgacaatgga gcaggaacag acgatagggt ttatcttaag
      721 gtttataatg ttgaaacggg ctggcgttat atacacctcg ataaaaatgg agacgacttc
      781 gagaatggca atgtagatac ctatacaacc tcaaataata caaacggcga tcctgtagta
      841 ataggtccag gcgatacgca ttttcgtatg tataaatcac cggatggcac cagtttcgga
      901 gactggaagc ttcaacattg gagcgcaaca gacaatggac aagtcatcat aaatgactcc
      961 cataatggct ggctaaacga tcagactacg cagaccccgg aacgagtcag gtgctatcac
     1021 tcgattggcg gaccaggaac tacgtgttaa tttagataaa atgaaaagga atataagcat
     1081 aattataata attgccgcct tcttgcttgg tttagcttat tttgttttca cgcgtagaag
     1141 tctagacaat atattgttgg tgcccccaga gctattaaat ccatcaacaa cagatgaacc
     1201 gccgctaata ggaggcaagg aagatggtgc aaagggtcag accgagccag atccgccacc
     1261 agaagatcag aaataatctt tttagttctt ttgacaagat gattatctct aaacggttag
     1321 taatcgcttt aatcatttcc tttctattgc tatcgatagc tttctattta tatagaccat
     1381 acaaaacttt tgaaatcgtt gtaaaaaaca atgaaattca atctttacaa gtcgggatgg
     1441 atgaatcgtc cgtaccaagg catttatatg tcaataaggg agattgggtg attattaaaa
     1501 taacagttga tcaagacagt agattatata tttctggtta cgataaggac atagatattg
     1561 agacggacaa ggaggcaaaa atctatttca aagcagatga tgttggtgta tttcaattag
     1621 aattaagtgg aagtagcgca gatctaggcg taatagaggt tctatcgaga taagagaatg
     1681 cacctcttat ttgtgatatg ttgacaacgg tcctattttt tgtatgattg tctacaaaaa
     1741 tcacctaatt atgacaataa agcctctttc taatcatatt ttccttgagt cacaggaaga
     1801 agaaaaaatt acaaagtctg ggatagttat tcctgatact gtagaaaaag aaagaccaat
     1861 tcaaggcaga gttttcgccg ttggtccggg caagctggac aacaatggta ggttgatgcc
     1921 gatatcggtt aaagtaggag accttgtgct ttttaagaaa tatggactgg atgaaataga
     1981 gattgagaat aaaaagtatt tagtcggaga tgaagacgat attcttgcga ttatcggtca
     2041 ataatcgcaa actctaagcg ctaagccata aattctaaac aatacctcta aacctaaatg
     2101 atcaaaatcc aaaaatattt agaatttaga acatttgaat ttatttagaa cttagatatt
     2161 agaaattaaa atttaaaaac aacatggcaa aaaatatttt attcaacgaa aacgcaagaa
     2221 atgccctgaa gaagggagta aacaagcttg ctgaagctgt taagatgact ttgggacctc
     2281 gtggacgagc ggccgtgata gaaaagagct atggggctcc gcaagttact tttgacggag
     2341 taacagtcgc caaagaaata gaacttgctg acagatttga gaatttggga gcggagctta
     2401 ttaaacaggc agcggataag accaacgata atgttggaga tggaaccaca acttcagttg
     2461 ttctggccca ttccatgatg accgagggag ataagattgt agagcagaag ggaataaacg
     2521 taattcaaat tgccaaagag ctcgagcggg ccggagaaaa agttgtaaaa atgcttgatg
     2581 agcaaaagga attgataaat gacaataaga aaatagaaga agtagcgtct ttgtctgcta
     2641 aagatgaaac aataggcaag ttaattgctg aggttatgag taaaataggc aaagaggggg
     2701 tcgtgacggt tgaagattct aatacggtcg caaattctta tgagatcgta gagggcatgc
     2761 aatttgatcg cggttacatc tctccttata tgatgacgaa tgccgagagg atggagtctg
     2821 cgattgaaga tccatatatt ttggttactg acaagaaaat ctcatcagtt aaagaattgc
     2881 ttccgattct ggagaaatta atgaatagcg gcaaaaaaga cttggttatt attgccgacg
     2941 acatcgatgg ggaagctcta gccactcttg ttgtaaataa attgagagga gtatttaatg
     3001 ttttagccgt taaagctccc ggatttggag acaggagaaa agaaatgtta gaagatatag
     3061 cgattgtgac cggagctaag tttatttcag agcagacagc tgaaaagctt gagaatacag
     3121 aattggattc tttgggaaga gctcatcgtg ttgtttctaa taaagataat acaactatcg
     3181 ttgatggtaa gggagttaaa gaagaaattg aaaacagaat cagtcagatt aaagcgcagc
     3241 tgaaaaaaac agactctgat tttgataaag acaaattaaa agagcgtttg ggaaaattaa
     3301 caggtggagt tgcggttatt aaagttggcg cgccaaccga atccgcacaa aaagaattaa
     3361 agcaaagagt tgaggatgcc gtatccgcga ctcgcgccgc tatggagggg gggatagttc
     3421 ccggaggagg ggtagcttta tttaatgcct atcttgccat gcagaatgag ataggggaga
     3481 ttaaagacga tgtggttgaa ggagcttatt ctattttaga gaaagcgtta gtctctccga
     3541 ttgaagcgat agcgataaat agcggagagt cggcgtccgc aattaaggat aagttgatga
     3601 acatgaaaaa gaaatcaaca tcacaagaca aaaattggat tggatttgat gctttttcag
     3661 gggagatagt agacgacttg agacaagagg gaattataga ccctcttaag gtgacaagag
     3721 aagctttttt gaatgcagta tctgttgctt caaattatct tacaatagga gccgcagtta
     3781 cagatatccc ggaaaaagat aagccggaaa tgggcggtct gcctggtcag ggaggaatgc
     3841 ctggcatggg aggattttag cgtagctgat tagttcaagt agtgtataat atagattatg
     3901 gatgcattgt atattttctt agcaatagcg acggtatggg ttatatggac ctataaccga
     3961 tttgttactt tgagatatag agccaaagag gctttatctg atatagacgt tcagactaag
     4021 aggcgatatg acctgattcc taatttagtt gatacggtta aaggttatat gactcatgag
     4081 aagagcgttt tagagaatgt cactaaggca agggcggatg ttgtcggcat gagcggagac
     4141 cagatcgata aggctgaagc tgaaaatatg ctttcaagca cgttaaaaac ccttttcgcg
     4201 gtcgcagaaa attacccaga tttaaaagct aatgctaact tcttggattt acaaagagag
     4261 cttgccgata cagaaaataa aatacaggcc gcaagacgtt tttataacac tacagttaga
     4321 gatttgaata ttaagattga tatttttccg tctaatttga tcgctaacct ttttagattt
     4381 aataaagaga agttttttga gacagaggtt gccgcagaga aagagccagt caaagtcagt
     4441 ttttagccac tagcgactag ccactagtag tgatagaatt ccttttgcgc tattggctaa
     4501 ccactagtcg ctgatcgcta ttcccatggc tagtttatat acccacaaag aatcaaatat
     4561 acgcaaaacc tttttcttat tttttatttt tctagttgtt gttatcggaa tcgggtgggc
     4621 tttcagttat gtctataatt cgtctttgat cctttggata gcggttattt ttagttctgt
     4681 tatggcgatt ataagctatt ggtactcgga taaaatcgtt cttcgaatgg caaacgctgt
     4741 gcccatagat atgaaaaata acccagagct ttataggatc gttgagaatt tatccattac
     4801 cgctggattg ccgatgccca gactttattt agtgcgcgag caggcgccga acgcttttgc
     4861 aaccggacgc aatcctcaaa attccgttgt cgcggttact gagggcttgt tgaataaact
     4921 ggatcggtct gaattagagg gggtgatcgc tcatgaatta tcacacatag gtaatcgtga
     4981 tatgttggtt gggactgtcg cggtaattct ggttggattt atttctttga ttgcggatat
     5041 gttcatgcgt tctttgttct ggggaggcat gaggagcagg gacaggggaa acggccacgc
     5101 agtcattatt tttattggaa tagttttatc aattctagcc ccgattgctg ctatgcttat
     5161 gcagcttgcg atatcgcgta agagagagtt gctcgctgat gcttctggtt cgctcttgac
     5221 tcgttatccg gaaggacttg caagcgcgct tattaaaatc tcaacagatt ttactccgat
     5281 gagagcggca aacaatacca ccgcgcatct ttggcttgat gacccgttta aaggcaagag
     5341 gaaaacttct tttttgcata aactttttat gacccacccg ccggtagaag atagagttag
     5401 ggcgcttcga ggcatacaag tataataaga caaaaaccgc ctagataatt tggcggttat
     5461 tttttaatct ggcttcaaat gatctttcat tctctggatt gaatatgtgc ctgacatcca
     5521 gaggcaggcc tgaatgaaac cgagccatcg atgcactttt tctaccctgc cctctttgat
     5581 aagttgcggt attttttgca acattctgtg gcaatgagcc agagcatccc tgttagatgg
     5641 tcttagctca ttgcgtttga agctttttgc gggtatgttc atctcttcaa tttttcttgt
     5701 ataaagagcg gtggcttcta ggagtttttc tgcattcatc tagcattcct ctatatagtt
     5761 agattatcta tatatgttta ttaggtcaat cgtccttata atataagttg cctagccgtc
     5821 tgttaattgc tataagttaa tagttattat ggagggttcg cctagcggct atggcacaat
     5881 gattgttccc gatttgttta aaaatccaag ctagcgtagg atttttaaac acaaatcgca
     5941 atcctgcaca aagcggagag gtgcgtgagc ggttgaaacg acagtcttgg aaagactgca
     6001 taccttaacc ggtatcgagg gttcgaatcc ctccctctcc gctttgtacg ggagaaagtg
     6061 gtttgccccg tagatcgctt cgctccacgg ggtaaaaagt cccacgccct ccgccaggca
     6121 aggaaatgga gttattggtt tgccacccac aacaatttat tataaagaat aagataaaat
     6181 aattttatga ccaaaaaaat aaccaaaggc gccctgcata aagttccatc ggacctaaga
     6241 gaagcttttt tttattctcc taaggcgctt gccgcatggc aagacataac actgttggcg
     6301 cgcaatgagt ggatttgctg ggctatctct gtaaagaaag acgagacgag gagagatcac
     6361 attcaaagga cgattagtga actcaaagaa ggaaagcgga ggccttgttg ttgggctggc
     6421 tgcccccatc gcgctaaata atttgaatcg gtgcacagcc ttgtgcgtca atggaagagt
     6481 taatgaaacg tcctaattct tttgccaaca tgatgaagat gaaaaaaatt gttattgagg
     6541 atttctaacc tcatgtttac tgatagtaac cataaaatat attagaagaa tcatgactac
     6601 tatgtttgca atcttaggcg atgtcattca ggcgcttggt ggattcaaat aatgatgtct
     6661 atttaaagat tcagagttgc tttcctaatg tgtcgtgata tgcggataaa ataatctttt
     6721 ttacttcggt aggctctttg atgtctttta taagaacctc tttgcttact ccagccgttt
     6781 gaatgtgtag attgccaaat cttaatagcg taggtatcaa tccgtttatt tctattttta
     6841 tatcttgaat attttggaga ggaacagtcg ccaagtctct cctaaacagt cccctttgtt
     6901 ctacgtcaac gactcttttg ttcgttacga tcaaaacgtc aagataatag tcggtccatg
     6961 ctataaaaaa actgacccac ataataagag tccacgaaag gataaaaaag acagcaatcc
     7021 aaataaattt tatatcttca aaaaaagcgg ttatttctgg aaatgcgccg agaataacaa
     7081 taacaaatat tatcggagca agtataacgc caaacattat ggctccggtc gtgcctataa
     7141 cgaaccaatg cctcctctgt tctaagagta ttttttcttc ttcttctagg ttgatcattg
     7201 agtgttatat attattaaaa tattaattat taatgccata aataatatgc ccgatactcc
     7261 aaaatacgtt tttcttaata atttaagact tttttgggtg acaccgtatt gattccagtg
     7321 atagtttaat atcgcgctga tcaatataaa aaacatcacc acaataacaa tccatatttg
     7381 catagccgtt atgaccgtct ccattgttat tggttgatca atcacattat aattataaca
     7441 ttaatgattt atgaattatg gtctcattta attaattcga tagattgagt tattattgaa
     7501 atcacacaaa catgttgttt ttttggatta tagttttcgc ggtttctatg gctgttcttg
     7561 ttaagggcgc cgattggctt ttgatcggag cggagaaagt gggtatagct ttaggaatcc
     7621 ctccttttat tatcggagtt acaatagtcg ccgccggcac atcgtttccg gagcttgtct
     7681 catcaatcgc agccgttatt aagggagcag aggaggtcgt agcggcgaat gtaataggtt
     7741 cgaatatcgc aaatatactt ttggtagttg gattgtcggc tgtttttgcc ggtaggttaa
     7801 ctgttacaaa aaatcttatt gatctcgatt tgcccctttt aatcctaagc acaacccttt
     7861 ttttaggagt tgtttgggac ggtttgataa cggtagggga atcaattatt ttggtttcta
     7921 catatcttat atatgccgtg tatactgcag ttcatcgtga tgatgaagaa gtattatcaa
     7981 aagaatccag ccccaaagat aagcctaagc tgaagattaa tgattttgtt ttcatcgcag
     8041 tgggtatagc ggggcttgtt gtgggctcta agtatcttat cgattcggtt attagccttt
     8101 cagaaatttt aaatgttgga aaaggcatta tagcgattac tgctgttgct ataggaacgt
     8161 ctttacccga acttttggtt tctgttaagg cggctattca taaaaagtct gaagtggcgc
     8221 tggggaatat ttttgggtca aatgttttta atgttcttgt cgtcgccggg atacctggcc
     8281 ttttgggaaa tcttccttta gatgaaaaaa cgtttttatt gggcgtgcct gcgcttgctg
     8341 ttacaacttt gatttttgtt atttccggga tatcaaaaag aatatatata tgggaaggtt
     8401 cctttttctt ggctatctac gctctttttc tgggaaagct ttttaacctt ttttaaagtg
     8461 attttctata ttgttgccaa gacttaagag ctggacttat agctgcgccc ggctttttca
     8521 acataggatt cctaattttg gtgtattttt ctttagagtg cttgaacatt ccggtaatcg
     8581 cttttttatt tttgcttagg atctcacgaa tagataagaa ttttttagct ttttcaatag
     8641 tgggtttgat tgatacagcg agaacgctac ctatcaatat ggcgccgttt tttattttta
     8701 tattttcttt ctgcctgaag atgtttgaac cggtcattat atattccatt tgctctactc
     8761 cgcgaaaata gtcatacatt tttttgtttc cgccccgtat cacactctga taagagttga
     8821 acatctcttt tttaagctcg ctgtctagat caagggagaa taagacgcgt tgaaacatat
     8881 ccgcctcttt ttcttcaagc tcttcgtttt tataaggatc ggatacatcg ccgattcctt
     8941 ccccgtttat tatagcctct ccaaagtcgt ggcatgctgc ggcaagcaat aattctcgat
     9001 gttcatctgc agtgaataac cctggattag aagtaagttc gcgatgaagg agataattta
     9061 caagcgcatg catatagatc atgtggctat atgttatcgc gcttggtccc agagccattt
     9121 tccatagtcc gatagattct gaatctctgt aaacattgaa gcggaccgta tttttaagcg
     9181 attctccagc cggcgtcttt ccaagcactt caaatatatg cgagatcggg attttaggta
     9241 ttaatttgtg gttcatatga aatcaattat ataacatatg gtatcattcc tcatatgaag
     9301 atagtcgcag atctgcatat ccattcgcca tattcaagag ctgtatctaa aaatatgacg
     9361 ctccaaaacc ttgattactt cgcgagatta aaggggataa atgtcatggg gacaggtgat
     9421 tttactcatc ccatgtggat tgacgaaatt caagagaaac tagagccagc tgaagctggt
     9481 ttatatattt tgaaggatga atatttgtac ccggagtcaa aaggtgagaa aacaagattc
     9541 atgttaacgg ttgaaatttc cagtatttat tctaaaggcg ggagagcgag aaggatacat
     9601 aatctaattt ttacgccgac gattgaagct gccttaaaga ttaacgctga acttggatgg
     9661 aggggcaata taaaatcaga cggccggccc attttaggta tggatcctaa agagatagca
     9721 aaaattattt tcaatattga gcccagatcg gcgattatcc cggcgcacgc ctggacaccc
     9781 tggttttcca ttttcggatc aatgtcagga tttgattcta tagaggagtg ctttgatgaa
     9841 tttgccccgc aaatttttgc tatagagacg ggactttcca gcgatccggc gatgaattgg
     9901 agattatcca agctcgataa cattgctctt atttcaaatt cagactctca cagcttgaaa
     9961 aaaataggtc gcgaagctaa tgtttttgat actcaattgt cttacgacgg cattattgag
    10021 gcgattaaat cacatgatcc taaaaaattt cttttcacgg ttgaattttt tcctgaagag
    10081 ggcaaatatc actacgatgg ccatcgcgta tgtaaatatt cgtgtgatcc agataagaca
    10141 aatgatcaca ataatttgtg ccctcgttgc ggaaagaaaa tgacgatagg ggttttaaac
    10201 agagtgagac agctggcgga tagggaatct ggatttgcgt ctaaaaacgc aataccattt
    10261 aagaatttaa tacctttgga tgaaatcata gcggatgtaa ttggcgttgg aaccgcatct
    10321 aaaaaagtta atgcggaata tatgaatttg attaataaaa taggaacaga attctccgcg
    10381 cttttggagg cgagtgagtc cgagcttgct tctgcctcgt ctcctcgtat cgcctctgcc
    10441 attacgatgg tccgtgaagg taaggttagg attgagccgg gttatgacgg cgaatatgga
    10501 aaaataggca taatgccaaa agaaggctct gaagagaaac aagagggtct ttttttaact
    10561 ggtttatagg ctttttatca gtttaccgct atttagctat tttgttttct tgttagctta
    10621 tatcgtcttt tctgcttttt ataaaccctc tgaaattttc taagaattct atgaatattt
    10681 ggaggggaag atctataatt aagttgaaaa caacaaccaa tatgtttaat ttgccgagcg
    10741 ccattaggcc ccatttgcct atggttataa atgggatagc aaaaagatca atcaaaaaac
    10801 tggatagcgt ggcttttttc ttttcaaggc ttatttcttt tgcctgccta ctgactctta
    10861 ctcccgccgc tgagaccagc gaagtgaaaa atatgaatac tactatattg gccaggctga
    10921 aatttataga aagcaatatc tttgaaatcg catataaaga gaaaaagaag gctgcgatat
    10981 aagaaagtcg aatcataata ttggttatga ctccttttct cttaggaata ttaataatat
    11041 attttttatt tttttgaccg gagacagcga tgtctatttc ttccataatg agatcaaagt
    11101 ttgatgacga cggcattttg atcgataaga ctatcataag
//
LOCUS       DATDNZ010000023        17553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3215493, whole genome shotgun sequence.
ACCESSION   DATDNZ010000023 DATDNZ010000000
VERSION     DATDNZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17553
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215493"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..2557
                     /locus_tag="VI432_00920"
     CDS             47..2557
                     /locus_tag="VI432_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY4496695.1"
                     /translation="MLTQEIKTKIDSARDILVGKVPDPKAQVEQITTALIYKFMDDMD
                     KQAASLPKGKPQFFTNGFEKYAWSKMMDAKLGGQERLNLYVEAIAKMAKNPHLPQLFR
                     DVFKDAFLPYRDPETLNLFLKDINTFSYDHSENLGDAFEYLLSVLGSQGDAGQFRTPR
                     HIIDFIVEVVDPKKDETILDPACGTAGFLISAYKHILKSNKNKPLTPDDKKRLMNNLA
                     GYDISPDMVKLSLVNMYLHGFPSPKIFEYDTLTSEEKWDERFDVMMANPPFMTPTGGI
                     RPHKRFMVQANRSEVLFVDYITEHLNNKGRAGIIVPEGIIFQSSGAYKQLRKSLVEDG
                     LYAVASLPSGVFNPYSGVRTSILFFDNQLAKQSKDILFIKVLNDGFDLGAQRRPIDRN
                     DLPHAVEVLQAQQEAIKTGKKNLLLKEGSLFAWAVPKTKIAESGDHNLSGDRYNHIAK
                     RLNHRWEITELGKLTEIFIDGNWIESKDQSSSGIRLIQTGNIGMGEYLDKGDKARFIS
                     EEKFEELGCTQVFAGDVLISRLPDPVGRACIVPEMHIKLITAVDCTIVRFDGKKLLPR
                     FFVFLTLTNQYYERIHQYLTGASRERISRSNLAKIQIPVPPLEVQKEIVDELGGYQKV
                     IDGAKQIIEHHKPSIKVDPTWERVELNNVAEIIGGFAFKSEKLSDTKSSDECLGVVKI
                     GNVSKSGNLDLSSIQYHKFSPDLTKFLIKKNDLLVAMTGATVGKVAYSEFDGLLLNQR
                     VGIIRAKEGTIQEYLKAILISDNFYNYCQSTAGGGAQGNISPSQIMRYEIPLPPIDEQ
                     KKIIAVVQAESRHIESAKQMIEIYEEKIKSKIAEVWGE"
     gene            2564..3007
                     /locus_tag="VI432_00925"
     CDS             2564..3007
                     /locus_tag="VI432_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496696.1"
                     /translation="MKLLTEKFVQDSIIEYLFRKGWSRNLRSKTTEEHGVDIKVRNNN
                     FSRYWLIECKGDASKNAKYPRSHREVCFNLALGQIITRMKSGGKRGYKYGYKYGVGFP
                     ASFRDLVVRRLPFNVADKLNLYVFLVDEKGKVELFDWVKLKNNQI"
     gene            3004..5598
                     /locus_tag="VI432_00930"
     CDS             3004..5598
                     /locus_tag="VI432_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496697.1"
                     /translation="MTTTLIPKTKASDYLQEYANQSNEEWLRFLISKVFILRTVPNNE
                     TLDEVYQKFLVEHKLRKSDAKEEETVTRIALLHKDSKAKSFVLKELQHEDGVNALERG
                     ATITFHQKLTVVYGKNGSGKSGFVRILKRLAGSRTQEDVWQNVHNSQSQNRCNAKVLH
                     ASRGTDSTYQWNGESKVTPFDQMAIFDGRCIPIYLNKSLDFSYQPYGFELFQLLSTSL
                     QSLQERLNAAIQSAERNKPLIDDIFNEETTIGKFIAGITSTTKVEDLNKLPVWDSKLQ
                     KELTDKQQERKGLQNLDQQSELLRTRHQKLKALEGALTETQSDLSAQSIRAYFGIAKK
                     FHQLKKKQATKKGKTLEDYDIAEKESDEWEKFIDAGEDYIRIAEHDDYPRDSDQCVYC
                     QQKLSKSAQKLVQLYRELFQEEETSDVEDVEAKLNEALEDLENPSYEEDFQYSQDDFE
                     KLLQKRTIAAAFAALKEADSLSQKLASVLKSKKPKKLSPLNTSGIVASIRKIRMKAEE
                     DIKTLEETQRNLFKKSKDLERVVAELQDIQKFSKRRSEIEKYISIERWVAKASPLLGS
                     KLNTKSITDLGKRAWRELVSDSFKERFQRETADLDAPKVNLDFRGEYGSQMRAKNMEG
                     LDEIDQFLSEGEQKAVALADFFAELSMQNEHAPVIFDDPATSFDYDRKERIAKRIAQE
                     SATRQIVVFTHDLMFASYLHDQVETSNGDVDSTKAAFHNLYSEANRVGIVDENYYPGS
                     VKFDTYMRKIEAKVAEVEALTAAARREGIKDAYGKLRKAVEKAVEERIFGGVITRWSD
                     QIQMHNATSSRASLSREKLDKAKQLHEEFSRYIEAHNQSDEMIQHAMPDLAKLKNDIQ
                     QVKEVAVR"
     gene            5610..5966
                     /locus_tag="VI432_00935"
     CDS             5610..5966
                     /locus_tag="VI432_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496698.1"
                     /translation="MLSKEAIDEFKKLYKEQYGEELNDFVASEAANRLVRMMDVVYRP
                     IPKAWEAEYNRIKAEQEAVPEEKKRDYVKHLVEDAKVTEFKEKIGEKNFWNLVVEANK
                     KQTEEKMRGEPSKKQG"
     gene            complement(5963..6631)
                     /locus_tag="VI432_00940"
     CDS             complement(5963..6631)
                     /locus_tag="VI432_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496699.1"
                     /translation="MADHNIQSENGNTKRFSFYESFDGHYQKIGELKELIPQFKAFYY
                     AERRKDQERSAIKIINDFNRQIAPLTFFPWEKQYRLWRKKWDAELITAQNYKQEQRQL
                     IKMQDEQSALIVPDEQTLEMGTKTLAGELLNDAMNILKGDQENGDIFDEEILIKRRSY
                     ILNVFNYVTKAVHGKEALKLKSNADKRETFGFLAALINRSTAGKITDDEMALLKQSIS
                     QDKS"
     gene            6829..7509
                     /locus_tag="VI432_00945"
     CDS             6829..7509
                     /locus_tag="VI432_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY4496700.1"
                     /translation="MNTFEIEIKSLLGSAENTNELIRKMKEKDPNLVQHETHKQLNHY
                     FIDGDLDKLFKKIEPFLKDDKKFENLKNIKNKIKDYTLRTRLADGKVILVFKTSINEE
                     ASANAKARLEFEWEIKNLTLDELDKIILDCGFKYQAKWSRERTNYKYRDVNVSIDKNA
                     GYGYIAEFEKVVEDGSLVDQIKKELLESMEELSVTELPQARLERMFAHYNANWRDYYG
                     TDRIFTIE"
     gene            7610..8563
                     /gene="thyA"
                     /locus_tag="VI432_00950"
     CDS             7610..8563
                     /gene="thyA"
                     /locus_tag="VI432_00950"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY4496701.1"
                     /translation="MQKHPEYQYLDILRDIMENGSEKPVFNNPGVTIKSVFGRQSRYD
                     LSQGFPLLTTKKVFLRGIIEELLWFLKGESNIKYLADRDVHIWDEWAYKPYKKAAEKG
                     GVEKMEQKDFITKIKALPADDEFVKKWGDLGNVYGVQWRKWKTSDGRTIDQLGWAIDE
                     LKTTPFRKSIVVSAWNPEFIYEMAAPGKSMTLPPCHTMFQFSTVEDRLSLHLYQRSCD
                     AFLGVPFNIASYALLTMMIAQVVGLKPGEFVHTYGDIHIYSNHIDQVKEQLIREPKPF
                     PKMLINPEVKNIDDYKFEDFTLEGYDPHPPIKAPVTVVGGF"
     gene            complement(8560..9243)
                     /locus_tag="VI432_00955"
     CDS             complement(8560..9243)
                     /locus_tag="VI432_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496702.1"
                     /translation="MRSNVFWGIAVTIAVLIVVFWGYISLNNLYISDKIDKESTPSAL
                     ETDNYLWTNNSDEFYLYTNERLGFSIEIPKSIAIYCGPPDLDCLDRGVVSIIEGEDSI
                     GLGASGFNMIFKQVRNESELANFIKDINGEHCESFSVEPNISYEGIFDIKVNSTDIGD
                     PSTCQAFFFQGFYSPKKERAVLIQVGQETQFQRTFIPCTTRKEDGIFACDPPPYYDLE
                     VVNSFRFLD"
     gene            complement(9259..9834)
                     /gene="dcd"
                     /locus_tag="VI432_00960"
     CDS             complement(9259..9834)
                     /gene="dcd"
                     /locus_tag="VI432_00960"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY4496703.1"
                     /translation="MILSDRDIKTAIQGGRIKITPEPDYNTQLGSCSIDLKLGNKFRI
                     FKNSLHPFIDVKGNIPVDDIMEEVVVPDGKPFVLHPGELALAMTEEVLEIGDDLIGRI
                     EGRSSLGRLGIIVHGTASVFDPGWNGHPVMELGNLGVMPVALYPGMRICAFTFEELSS
                     PAEVPYNKHKDKKYIGQSSPMASQLNKDFKK"
     gene            complement(9918..10334)
                     /locus_tag="VI432_00965"
     CDS             complement(9918..10334)
                     /locus_tag="VI432_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496704.1"
                     /translation="MDTTFDIADKKVLILEDERPLSTAIEEKLKMTGLPSISARSVEE
                     GLSYIENENIGLVWLDHYLLGKRNGLDFVAELNNVHRDKNIPVFVVSNTASPDKKDAY
                     LAMGVEKFYTKADHRLDNIISDIKGVLDGERKRVEA"
     gene            complement(10362..10736)
                     /locus_tag="VI432_00970"
     CDS             complement(10362..10736)
                     /locus_tag="VI432_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496705.1"
                     /translation="MKKKVLLVEDVKAVREALHEKMEMEGLEVIDAVDGEEGLKKFSE
                     EKPDLILLDIIMPKMDGITMLRELRQRAEGKDAPVIILTNLSDADSIEASIKSGVSDY
                     LVKADWKIGDLMEKIKQRLGSS"
     gene            complement(10751..11137)
                     /locus_tag="VI432_00975"
     CDS             complement(10751..11137)
                     /locus_tag="VI432_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496706.1"
                     /translation="MAQDKIVLVVEDERPLLEAISKKLESQGILAVTARSVDQAASYL
                     EDVKGVNLIWLDHYLLGPKDGIDFVNDLKRDAYKKDIPIFVVSNTASPDKRDEYIKLG
                     VDRVYTKADYKLDDIIKEIKLFLEKK"
     gene            complement(11124..11384)
                     /locus_tag="VI432_00980"
     CDS             complement(11124..11384)
                     /locus_tag="VI432_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496707.1"
                     /translation="MYKELVTITREYLGPAADRFVERQVKSHLENKAEDVLSQKDDLN
                     KEDIENLSNWFKVSISMMTDDRKTVDDFIRKINELNNKNGSR"
     gene            complement(11408..12343)
                     /locus_tag="VI432_00985"
     CDS             complement(11408..12343)
                     /locus_tag="VI432_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496708.1"
                     /translation="MKYLANFSKGVKLYGALSILLWLSYMVLTFNAPRSESRLIANLT
                     DLQIFYLQISVVLPILLIWVFGFLAVFALDTYIRKIKGSEDASALKFIYWGVAILVFN
                     LVFNSFLSSIASYYRDNESLMINFARIRNYISVFIPIISFYFISLGSKKLLDLIKYAK
                     DLWTQSASYIIASLSGILFAYLTFSYNLNRNMPSESDVQSYWLSDPMIALTIILPYAI
                     GWFFAIKAIIDFYLYSKNIQGIIFKEAFSQFAKGFLTVTSLTIALQILSAAQAFAEAN
                     LPGILILLYIIIIILAVGYFYIMSGSRKLLTIEKL"
     gene            complement(12401..15025)
                     /locus_tag="VI432_00990"
     CDS             complement(12401..15025)
                     /locus_tag="VI432_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4496709.1"
                     /translation="MRAPIIIALLINIIVIISATIYALLGILGYATVSIFLFLIWLAA
                     LLFFNSLFIYLFWKNRGDFNYEHQALVILWGGISTWYWSFAPIYYLLNMNLDELQLAW
                     VMFAYFWEVPIIGGGLFVFISYLFFRPFGRYLSGKTKIGNPEKFLNRIFSLPLKIAIL
                     IFGVAVVGYIIGTLQLKYFAYLSFVEQIKNVLNGVSISVFIAIALYLVFDYFLSPVRA
                     KFQKDFGKPKKTRRKIFYKIFIASTFLAIGISMMISLMVFKSFQEILFEEAESVLSGN
                     SSELNLRLSSVAGFTERKSILESYKGLYNTRSFYLEEEEEEEIPYISLLSEDTLSFVR
                     RGESGFERDFKNELRLVGLFEGPFEGEKIISVFEIKELYVRTTQYIYQFIIGLLFAVS
                     LTILLGLLISVFLTRSIGKITNHLRLNPKESLEDNPILYTGDDIEDLFHAISGYIEEA
                     RNSQAILQEKVDDATLELSARVEESEESKKAMENVLEDLGSARDKLELAQIEDEAILS
                     SVGVALLSTDEYGKITKANGEMISISGLGKDEIIGKWIHDALPLIDLKKGIKIDSEDS
                     PTTESLTSGRPVSGNYKIINKYKGEIPIFINASPIKVGDRPIGIVLIVRDITVEQEID
                     KVKTEFVSLASHQLRTPLSAVKWYSEMLLAGDAGEITETQKRYLDEVYNSNERMIELV
                     GALLDVSRLELGTFSVEPENILITDIAEDLIKENEHLIDKKNIKFKTDFDKDLAPAML
                     DKKLIGIIIQNLLSNALKYTPNGGSVSLDIKLVAIVNKKIEKGKDQILIKVSDSGYGI
                     PKSQQSKIFTKLFRADNVRSKDTEGTGLGLYIVKSILETAGGDIWFDSVENEGSTFYV
                     IIPVTGMAGKKGSRRLGE"
     gene            complement(15030..15794)
                     /locus_tag="VI432_00995"
     CDS             complement(15030..15794)
                     /locus_tag="VI432_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496710.1"
                     /translation="MMQLTVIIKYLVTPILIGIGLAYFGPQLTYSSSPSGSISISAWI
                     LSGFFFAVGLIAAFLIYRERKVFLASGNSLPFNIAIGCVILAIFSISAAATFFHNDRD
                     TIGWLVQVGTLASAAWIFYVSFAKREIIASERRDDFVFSYSMLAVVISSAVTWLLFIF
                     KDFMPLVLDPNFGWGKELLWVRVISSVLFLLAGTIFFIRKEQALIVGAIFLLAVGSFG
                     VVYVYSSGILTTHPLIWFNRVLTLLALLALLKRPNQ"
     gene            complement(15863..16234)
                     /locus_tag="VI432_01000"
     CDS             complement(15863..16234)
                     /locus_tag="VI432_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496711.1"
                     /translation="MKILVVEDEITLAEALRAKFEKKNYDVRVVSDGGAALASTQSFK
                     PDIILLDLLLPNKNGFEILQDIKADVTLKNIPVIILSNLATDEDIKKAISLGAVDYFV
                     KADHPIQEVLEKVEKQLLKSR"
     gene            complement(16272..>17553)
                     /locus_tag="VI432_01005"
     CDS             complement(16272..>17553)
                     /locus_tag="VI432_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496712.1"
                     /translation="SALAAEEKIAESTHLEREFRDTALLEERKRAKTAIKESAKYKSA
                     LSSLRLKQAEQIKTEREKARLAIRDEKKRAREAMLVEKGKESYRIKAERERGRNLILA
                     EREKFRKEIVKIKNDADLRISEKDEEAKGIATAKKETASSIIILNKKIKDLQAKERSE
                     KQKMLGAKRDLARDIKKARHEARSRAEALAVHKQKSRSTLAGEKMKVKMAIAHEKDKT
                     AVILKEDEKRDREVMINERKREAEKLRAERRKMRVILKEEIQKKQKTLREMNKVNREL
                     RELKQQKAKKLREEIKKAKEAISRKEEKEETRMRTEEEKSKKNLLIERKKAKAALLRI
                     KKTEEILESVRKKAREALVSAKKRAASALLKERSKARSAIEAEKARTKTILAEDELHD
                     QEILDKLLEEKRKAEIEKKKAEDIIAKIRKQAGK"
BASE COUNT         5434 a         3676 c         3335 g         5108 t
ORIGIN      
        1 ccgaatatgt gaaggattat gtttcgctta acgcatatat gtaattatgt tgactcaaga
       61 aattaaaact aaaattgatt cggcgaggga tattctggtt ggtaaagtcc ctgacccgaa
      121 agcccaggtg gagcaaatca ccacggcgct tatctacaag tttatggatg atatggataa
      181 gcaagccgct tctctgccaa agggtaagcc gcagtttttt acgaacggct ttgaaaagta
      241 cgcttggagc aagatgatgg acgcaaaact tggcgggcag gaacgcctga atctgtatgt
      301 agaagctatt gcgaagatgg cgaaaaatcc ccacttgccg cagttgttcc gcgatgtctt
      361 caaagatgct tttctgccgt accgcgaccc agaaacactc aacctatttt tgaaagatat
      421 caatactttc tcatacgacc acagcgagaa tctcggcgat gcttttgaat acttactttc
      481 tgttctcggt tcacagggtg atgctgggca attcagaaca ccaagacaca tcattgattt
      541 catagttgaa gttgttgacc ccaagaaaga tgaaaccatc cttgaccccg cgtgcggcac
      601 ggcaggattt ctcatttccg cctacaagca cattctcaaa tcaaacaaga ataaaccact
      661 cacgcctgat gacaagaagc gcttgatgaa taatttggct ggctacgaca tatcgcccga
      721 tatggtgaag ctctcattgg taaatatgta cttacacggc ttcccaagcc ccaaaatctt
      781 tgaatacgac acgttgacga gcgaagagaa gtgggacgag cgcttcgacg taatgatggc
      841 aaatccgccc tttatgacac cgactggcgg tattcgccca cacaagcgct ttatggtgca
      901 ggcgaaccgc tccgaagttc tcttcgtgga ttacattaca gagcatctga acaataaggg
      961 gcgtgcgggc attatcgtac ctgaaggaat tatttttcaa tcatcgggtg cgtataagca
     1021 attacgcaaa tcgcttgtcg aagatggatt gtatgcagtg gcttcgttac cttctggggt
     1081 ttttaatcct tactctggcg tgagaacaag tattctattt tttgataatc aactcgcaaa
     1141 gcaatccaaa gatattttat tcatcaaagt tttgaatgat ggctttgatt tgggtgctca
     1201 acgacgaccg atagaccgca atgacctgcc gcacgccgta gaggttctgc aagcgcagca
     1261 ggaagcaatc aaaactggca agaagaatct tttgctcaaa gaaggcagtt tatttgcgtg
     1321 ggcagtacca aagaccaaaa tcgcagagag tggcgaccac aatctttctg gcgatagata
     1381 caatcacatc gctaagaggt taaaccatag atgggaaatt acagaacttg gcaaactcac
     1441 ggagattttc attgatggaa actggattga aagtaaagac caatcaagtt ctggcataag
     1501 attgattcaa actgggaata tcggaatggg tgaatatctt gataaaggag ataaggcaag
     1561 gttcatttca gaagagaagt tcgaagaact tggatgcaca caggtttttg caggagatgt
     1621 tttaatttca agattacccg acccagtagg tagggcttgt atcgttcctg aaatgcacat
     1681 aaagctgata acggctgttg attgtacaat cgtgcgattc gatgggaaaa aattactccc
     1741 aagatttttt gtattcttaa ccttgactaa tcagtattat gaaagaatac accagtatct
     1801 tacgggtgca agcagagaga gaatcagcag gtctaatctt gcaaagattc aaatcccagt
     1861 tccaccgctt gaagttcaga aagaaatagt tgacgaactc ggcggctatc aaaaagttat
     1921 tgatggcgcg aagcaaatca ttgagcacca caagccgtca atcaaagttg accccacgtg
     1981 ggaaagggtt gagttgaata atgtcgccga aattatcggt ggctttgcct tcaagagcga
     2041 gaagttatca gataccaaat cctctgatga atgtttggga gttgtgaaaa ttggcaacgt
     2101 ttcaaaaagc ggaaacttgg atttgtcctc aatccagtat cataagttct cgcctgacct
     2161 gacaaagttt cttatcaaga agaacgactt actggttgcg atgactgggg caactgttgg
     2221 caaggttgct tattcagagt ttgatggatt gcttctgaat caacgagttg gaattattag
     2281 agcgaaagaa ggcactatac aagagtatct caaagcaatt ttgatttctg ataatttcta
     2341 caattattgc caaagtactg ctggcggtgg cgcacagggc aacatttcac cttcgcagat
     2401 tatgcgctat gagattcctt tacccccgat tgatgagcaa aaaaagatta ttgctgttgt
     2461 tcaagcagaa tcaagacata tcgaatcagc caagcagatg attgagattt acgaggagaa
     2521 aataaaatcc aaaatcgccg aagtatgggg cgaataaacc actatgaaat tacttaccga
     2581 aaagtttgtc caggacagta tcattgaata tctattccgc aagggatgga gcaggaactt
     2641 acgcagtaaa accactgaag agcacggcgt tgacatcaaa gttagaaaca ataacttttc
     2701 gcgctattgg ctcatcgagt gcaagggcga tgcgtccaag aacgcaaagt atcctcgctc
     2761 tcaccgcgag gtttgtttta atttggctct cggacaaatc attacgagga tgaaaagcgg
     2821 cgggaaacgc ggatataagt atggctataa gtacggggtt ggctttcctg catcattccg
     2881 cgatttagtg gttcggcgct tgcctttcaa tgtagccgac aaactgaatc tctatgtctt
     2941 tttggtggac gaaaaaggca aggttgaact gtttgattgg gtaaaactca aaaataatca
     3001 aatatgacca ccacgcttat tccaaaaact aaagcgtctg attatttgca agagtatgcc
     3061 aatcaaagca atgaagaatg gttgcgattt ctaatcagta aggtcttcat attacgaact
     3121 gttcccaaca acgaaacctt agacgaagtt tatcaaaagt ttttagttga gcataaatta
     3181 agaaaatctg atgcaaaaga ggaagaaacc gtaacgagaa tagcattact tcacaaagat
     3241 tcaaaagcaa aaagttttgt tttgaaggaa ttacaacacg aggacggcgt taatgcgctt
     3301 gaacgtgggg caacaatcac attccaccaa aagttgactg ttgtatatgg caaaaacgga
     3361 agcggcaaat ctggctttgt acgaatactc aaacgacttg ctggctctcg aacccaggaa
     3421 gacgtgtggc aaaacgttca taattcgcaa agtcaaaatc gttgtaatgc aaaagtgttg
     3481 cacgcgagca ggggtacgga ttcaacctat caatggaatg gcgagagcaa agtaacgccg
     3541 tttgaccaga tggcaatttt tgacggaagg tgtatcccaa tctacctgaa caaaagcttg
     3601 gatttttctt atcaaccata cggatttgaa ctgttccagt tgctctccac ctcattacag
     3661 agcttgcagg aacgactaaa tgcggctatt cagtcggcag agagaaataa acccttgatt
     3721 gacgacattt ttaatgagga aaccaccatc ggaaagttta tcgcggggat tacatcaaca
     3781 accaaagttg aagatttgaa taaacttccc gtctgggact ccaagttgca aaaagaactt
     3841 accgacaagc aacaagaacg gaaagggctt caaaatctcg accagcaatc ggaactccta
     3901 cgcacgaggc atcaaaaact caaagcgtta gagggtgcgc ttacagaaac tcaatctgac
     3961 ttatccgctc aaagcatcag agcttacttt ggtatcgcca agaagtttca ccaactcaaa
     4021 aagaagcaag ctaccaagaa gggaaaaacg cttgaagatt atgacatagc agagaaggaa
     4081 tcggacgaat gggaaaagtt cattgatgct ggagaagatt acatccgtat tgcagaacac
     4141 gatgattacc cacgcgatag cgaccaatgt gtgtattgcc aacagaaact ctcaaagagt
     4201 gcgcagaagt tggttcagct ttatcgtgaa cttttccagg aggaggaaac ctctgatgtt
     4261 gaagatgttg aggcaaaact caacgaggcg ctggaggact tggagaatcc ttcttatgag
     4321 gaggatttcc aatacagtca agacgacttc gagaaattgt tacagaagcg gactattgcg
     4381 gcagcgtttg ccgcgctaaa ggaagctgat agtttgtccc aaaaactcgc aagtgttctg
     4441 aagagcaaaa agccaaaaaa actttcaccc ttgaatacct ctggcatcgt tgcaagcatc
     4501 cgcaagatac ggatgaaggc agaagaagac atcaagacac tcgaagaaac tcaacgcaat
     4561 ctcttcaaaa aatcaaaaga ccttgaacgt gtggtagctg aattgcaaga tattcagaag
     4621 ttctccaagc gtcgctcgga aattgaaaag tacatctcga tagagcgctg ggtagcaaaa
     4681 gcatctccat tacttgggag caagctcaat accaagtcaa ttactgacct cggaaagaga
     4741 gcttggcgcg agttggtttc tgattcattc aaagaacgat tccagcgtga aacagcagac
     4801 ctcgacgctc caaaagtgaa cctcgacttt cgaggagaat atggctccca gatgcgagca
     4861 aagaatatgg agggattaga tgaaattgac caatttttga gtgagggcga gcaaaaagct
     4921 gttgctctcg ctgatttctt tgcggagctt tcgatgcaaa acgaacacgc tccagtgatt
     4981 tttgacgacc cagctacgag ctttgattac gataggaaag agagaatagc aaaaagaatc
     5041 gcgcaagaat cagcgacgcg ccagattgtt gtcttcactc acgacctaat gtttgcgagt
     5101 taccttcacg accaggttga gaccagtaat ggagacgttg attctacaaa agccgcattt
     5161 cataacttgt attcggaagc aaatcgcgtt gggattgttg acgagaatta ctatcctggc
     5221 tctgtaaagt ttgatactta tatgaggaaa attgaggcga aagtagctga agttgaagcg
     5281 cttacggctg cggcgcgaag agaaggtatt aaggatgcct acggaaaatt acgtaaagcg
     5341 gtagaaaaag ccgttgagga aagaatcttt ggcggtgtta tcacgcgctg gtctgaccag
     5401 attcagatgc acaacgctac gtcctcgcgt gccagtttga gccgagaaaa attggacaaa
     5461 gcaaaacaac tacacgagga gttcagtaga tacatcgaag cgcataatca atctgacgag
     5521 atgattcagc acgctatgcc agatttggcg aagttaaaaa atgatattca acaagtaaag
     5581 gaagtagccg taagataaac ataaaatcta tgctttcaaa agaagccatt gatgagttca
     5641 aaaaattgta caaagaacag tacggcgaag agctgaacga ctttgttgca tcggaagctg
     5701 ctaatcggtt ggtgcggatg atggatgttg tgtacagacc aatcccgaaa gcgtgggaag
     5761 cagagtacaa caggattaag gcggagcaag aagctgtgcc agaagagaaa aaacgggact
     5821 atgtgaagca tctcgttgaa gatgctaaag ttaccgagtt caaagaaaaa atcggcgaga
     5881 agaacttttg gaacttggtg gtagaggcga acaaaaaaca gaccgaggag aaaatgaggg
     5941 gtgaaccgtc gaaaaagcag ggctaacttt tgtcctggct gattgactgt tttagtagcg
     6001 ccatttcatc gtctgtgatt ttacccgccg ttgacctatt gataagagcc gcgagaaagc
     6061 cgaatgtctc cctcttgtct gcgtttgatt tgagtttgag cgcctctttt ccgtgaactg
     6121 ctttcgtaac gtagttgaag acgttgagaa tatagcttct acgcttaata agaatctcct
     6181 cgtcaaaaat gtccccattc tcctggtctc ctttgaggat attcatcgca tcattcaaaa
     6241 gctcgcccgc aagtgtcttt gtacccattt caagcgtttg ctcgtctggc acaataagag
     6301 cgctctgttc atcctgcatt ttgatgagtt ggcgctgttc ctgcttgtag ttctgtgctg
     6361 tgataagttc cgcgtcccac ttcttgcgcc ataggcgata ttgcttctcc caggggaaga
     6421 aggtaagcgg agcgatttgg cgattgaagt cattgataat cttaattgca ctgcgttctt
     6481 ggtcttttct gcgctcggca taatagaacg ctttgaactg cgggatgagt tctttcagct
     6541 cgccaatctt ctggtaatgc ccgtcgaagc tctcgtagaa agagaatcgc ttggtattac
     6601 cgttctcgct ttgtatgtta tggtctgcca tagaaagagg gtgttattac gtgttatact
     6661 gcgactattg gcgaataaac aggttgaaga actccctgcc aaacttggag agattctatg
     6721 atttggttcc aattcaccct gacgtagtcc accatttcag atttgccgaa gaagttgcct
     6781 caaaaactaa aattagctct ttattttttt atgtaataaa attgtcttat gaacactttc
     6841 gaaatagaga ttaaaagtct tttgggcagc gctgaaaata ctaatgaact tatccgtaag
     6901 atgaaggaga aagatccaaa tctcgttcaa cacgaaaccc ataaacaact aaatcactat
     6961 tttattgatg gagacttgga taaattattt aaaaaaatag agccattcct caaagacgat
     7021 aaaaaatttg aaaatctaaa aaacataaaa aataaaatca aagactacac tctccgaaca
     7081 cgtctcgctg acggaaaagt tattttagtt tttaaaactt cgatcaacga agaagcaagc
     7141 gcgaatgcta aagctcggct tgaatttgaa tgggaaataa aaaatcttac cctggatgaa
     7201 ttagataaaa taatcttaga ttgcggattt aaatatcaag ccaaatggtc tcgagaaaga
     7261 actaactata aatacagaga tgtaaatgta tctattgata aaaatgcagg atatggatac
     7321 atcgctgaat tcgaaaaagt agtagaagac ggatctttgg tcgatcagat taaaaaagaa
     7381 ttgctagaga gcatggaaga attaagtgtt acagaattgc ctcaggctcg gttagaaaga
     7441 atgtttgctc attataacgc caactggaga gattattacg gaacggacag aatttttact
     7501 attgagtaaa attctgtaag tataaagtat cttgtattaa gtatttagag aaagcagttt
     7561 gtgaatttat ataccaaata ctatatactt catactaaat actatcgcca tgcagaagca
     7621 tcccgaatac caatatttag atatcctcag agacataatg gagaacggct cggaaaaacc
     7681 cgtcttcaat aatccggggg ttacaatcaa aagtgttttc ggcaggcaat caagatacga
     7741 tctttcgcaa gggtttcctt tattaaccac aaaaaaggtt tttttacggg gaattattga
     7801 agagcttcta tggtttctga agggcgaatc aaatataaaa tatcttgcgg atcgcgacgt
     7861 ccacatttgg gatgaatggg cgtataaacc gtataaaaaa gcggctgaaa aaggcggggt
     7921 ggaaaaaatg gaacaaaagg acttcatcac aaaaattaag gccctgccgg cggatgatga
     7981 attcgttaaa aagtggggcg acctgggaaa tgtttacggc gttcaatgga gaaaatggaa
     8041 aacctcggac gggcggacaa tagaccaact cggttgggcg atagatgaac tcaaaaccac
     8101 accgtttaga aaatccatcg ttgtctcggc ttggaacccg gaatttattt atgagatggc
     8161 cgcccccggg aaatctatga ccctccctcc ctgccacaca atgtttcaat ttagcacggt
     8221 ggaggatagg ttgtctctac atctatatca aagaagttgt gatgcattct tgggagtccc
     8281 cttcaatatc gcaagctatg ccctcttgac tatgatgata gcccaagtcg tcggattaaa
     8341 accaggagaa ttcgttcata catatggcga catacatata tacagcaatc atattgacca
     8401 agtaaaagaa cagctaataa gagaaccaaa accattcccc aaaatgttga taaatccgga
     8461 ggttaaaaat attgatgatt ataaatttga agactttacg ctagaaggat acgatccaca
     8521 tccaccaatc aaagctcctg taactgtagt cgggggattt taatctaaaa atctgaaact
     8581 attaacaact tccaagtcat aatagggtgg tgggtcacac gcaaagatcc cgtcttcttt
     8641 tcttgttgta cagggaataa aagttcgctg aaactgagtt tcttgaccga cctgaatgag
     8701 aactgctctt tcttttttag gactataaaa tccctggaaa aagaatgcct gacaagtact
     8761 aggatctcct atgtctgttg aatttacttt tatatcgaaa atcccctcgt aagaaatatt
     8821 gggctccacc gaaaaagatt cacaatgttc tccatttatg tctttaataa aattagcgag
     8881 ttcactctcg tttcgtacct gtttaaatat catattaaaa ccactggcac caagaccgat
     8941 ggaatcttct ccttctatga tactcaccac ccctctatcg agacagtcca gatcgggggg
     9001 accacaatat atggctatgg atttgggaat ctctatcgaa aaacccaatc tctcattagt
     9061 atataaataa aattcgtcac tgttattagt ccataaataa ttatccgtct ctaaagccga
     9121 aggagtagat tctttatcta tcttgtcgga aatatataga ttatttaaag aaatataacc
     9181 ccagaaaaca actatcaata ctgctatggt cacggctatc ccccaaaata catttgatct
     9241 catgctaatt atttcgagtt atttcttaaa gtctttattt aactgagagg ccattgggct
     9301 actctgcccg atgtattttt tatccttgtg tttgttataa ggaacttcag ctggagatga
     9361 aagctcttca aaagtaaatg cgcatatccg catgcccggg taaagcgcta ctggcataac
     9421 ccccaagttt ccaagctcca taaccggatg tccattccaa cccgggtcaa aaacagacgc
     9481 agtgccatga acgattatcc cgaggcgtcc gagcgagctc ctgccctcta ttctacctat
     9541 taaatcgtct cctatttcca gaacctcttc ggtcattgca agagcaagtt cacccggatg
     9601 caatacgaac ggcttgccat ctgggacgac aacttcttcc ataatatcat cgacgggaat
     9661 attgcccttt acatcaataa acggatgcaa agaatttttg aaaattctga atttattacc
     9721 gagctttaag tcaattgaac acgatccgag ctgagtatta taatcaggct ctggagttat
     9781 ttttatcctc cccccttgaa tcgccgtctt aatatctcta tctgataaaa tcataggttt
     9841 aattatataa aaaatctcta tttaataaaa tatccatttg tgagcgcgaa ttacgattta
     9901 attataatat caaaactcta agcctcaact ctctttcgtt ctccatccaa tactccttta
     9961 atgtcactta taatattatc tagccggtga tcggctttgg tataaaattt ttcaacaccc
    10021 atcgctaaat aggcgtcttt tttatcaggt gatgcagtat tagacacaac aaatactggt
    10081 atattcttat cgcgatgaac attattaagc tccgcgacaa aatccaatcc attcctttta
    10141 cccaataaat aatgatccag ccacactaag ccgatatttt cattttctat ataagacaac
    10201 ccctcttcaa ctgacctcgc agatattgac ggcaatcccg tcatcttaag tttctcttct
    10261 atagcggttg agagcggacg ctcatcttct aaaattaaaa cttttttatc cgctatatca
    10321 aaagtggtat ccatttttat aatcaaattt tttattcgga atcaagaact gcctaaccgt
    10381 tgtttaattt tttccattaa atcgcctatc ttccagtcgg cttttaccaa ataatcggaa
    10441 accccgctct taattgaagc ctcaatactg tctgcatcgc tcaggttcgt taaaattata
    10501 accggcgcgt ccttaccctc tgctctttga cgaagctcac gaagcatcgt tatgccatcc
    10561 atcttaggca taataatatc caataaaatc aggtctggct tttcctcgct aaattttttt
    10621 aaaccctctt cgccatcaac agcgtcaata acctcaaggc cttccatctc cattttttca
    10681 tgcaaagctt cgcgaacagc ttttacgtct tcaactaata aaactttttt cttcatgtga
    10741 atattatcct ctatttcttt tctaaaaaca actttatttc cttgattata tcatccaatt
    10801 tataatcggc ttttgtatat acgcggtcaa cacccaattt tatatattcg tctcttttat
    10861 ccggtgaagc agtattggaa acaacaaata ttggtatatc tttcttatac gcgtctcttt
    10921 ttagatcatt tacaaaatca atgccatctt ttgggccaag aagataatga tctaaccaaa
    10981 taagattcac tccctttaca tcttctaaat aagacgcagc ctgatctacc gaacgcgcag
    11041 ttacagcaag aattccttgg ctttccaact ttttgctgat cgcctccaaa agagggcgtt
    11101 catcttctac aactaaaaca atcttatctt gagccattct tattatttaa ttcatttatt
    11161 ttcctaataa aatcgtccac tgttttacga tcatcagtca tcatgctgat agaaacctta
    11221 aaccagttgc ttaaattctc tatatcttcc ttgttcaggt catctttttg agaaagcaca
    11281 tcttccgctt tattctccaa atgcgattta acctgacgct caacaaagcg gtcggcggcg
    11341 ggtccaagat attctctagt tatagttacc agttctttat acatactatt tgccatttat
    11401 cataaattta tagtttttct atagtaagca actttcttga gccgctcata atataaaaat
    11461 acccaacggc aagaatgata ataatgatat ataaaagaat gagtatgcct ggtaaattcg
    11521 cctcagcaaa agcctgcgcc gcagataaaa tctgaagagc tatagtgaga gacgtcaccg
    11581 tcaagaagcc tttggcaaat tggctgaaag cttctttaaa aataatcccc tgtatatttt
    11641 tgctatataa ataaaagtca ataatggctt taattgcaaa aaaccagccg attgcataag
    11701 gcaatattat ggttaaagca atcatcggat cgcttagcca atatgattgc acatccgact
    11761 ccgatggcat attcctgttt aggttataag aaaacgtaag gtaggcgaac aaaatgccgg
    11821 aaagtgaagc tattatgtac gaggctgact gtgtccatag atctttggcg tatttgatta
    11881 aatcaagcaa tttctttgac cctagagata taaagtaaaa agaaataatc ggtataaaaa
    11941 cagatatgta atttcttatc cttgcgaaat taatcattaa tgactcattg tccctataat
    12001 acgaagcaat tgagcttaag aaagaattaa aaacgaggtt aaacactaaa atagctactc
    12061 cccagtatat aaatttaaga gcggatgcgt cctccgatcc ttttatcttc ctaatataag
    12121 tatctaaagc gaaaaccgct aaaaatccga atacccaaat aagcaaaatg ggcaagacga
    12181 cagatatctg aagataaaaa atctgcagat ctgtaaggtt cgctatgaga cgagactctg
    12241 aacgcggtgc attaaaggtg agcaccatat aagaaagcca caataaaatg cttaatgctc
    12301 cgtacaattt aaccccttta gaaaaattag ctaaatactt cattataaat tttctaaatt
    12361 ttcatatatt ataccacatt agcgaaacca gaccataaag ttattcacct aagcggcgag
    12421 accccttttt acccgccatt cccgtgaccg gtattatcac ataaaatgtg cttccctcgt
    12481 tttcaaccga atcaaaccat atatcgccac ccgccgtttc taaaatagat ttcacgatat
    12541 aaaggcctag accagttcct tctgtgtctt tgcttctaac attatcagct ctaaaaagct
    12601 tggtaaaaat ctttgattgt tgtgatttcg gaatcccgta accgctatca gaaaccttta
    12661 tcaaaatttg atctttgcct ttttctattt ttttattaac aatcgcgact aattttatat
    12721 ctagactcac actcccacca ttaggagtat acttcaaggc atttgagaga agattttgaa
    12781 taataatgcc gatcaacttt ttatcaagca tcgccggagc caaatctttg tcaaaatcgg
    12841 ttttaaattt aatgtttttc ttatctatca aatgttcgtt ttctttaatc agatcttctg
    12901 cgatatccgt aatcaaaata ttttccggct caacgctaaa cgtgccaagt tcaagacgcg
    12961 atacatcaag aagcgcgccg acaagctcta tcattctttc attgctatta taaacctcat
    13021 ccaaatatct tttctgcgtc tctgttatct cacccgcatc accggcaaga agcatctccg
    13081 aataccactt aacagcggac agaggcgttc ttaactggtg ggaagcaagc gatacgaatt
    13141 cagtttttac tttatctatt tcttgttcga ctgttatgtc gcggacaatc aaaacaattc
    13201 ctatcggcct atcgcccacc ttaatcggag aggcattaat aaaaattgga atctctccct
    13261 tgtatttatt aataatttta taattacctg agacaggcct ccctgaagta agagactccg
    13321 tcgttgggga atcttctgaa tcgattttta ttcctttttt caaatcaatc aacggtaagg
    13381 cgtcatgaat ccattttcca ataatctcat ccttgccaag ccccgatata gaaatcatct
    13441 ctccgttcgc tttagttatc ttgccatatt catcagtact caaaagagcg acgccaacac
    13501 tagataatat agcctcatct tcaatttgag ccagctccaa tttatctctt gccgaaccta
    13561 agtcttctag cacgttttcc atcgccttct tactctcttc agattcctcg actcgcgcgc
    13621 tcaattctaa tgttgcatca tcaacttttt cttgaagaat cgcctgtgag tttcttgctt
    13681 cttctatata tcctgatatg gcatggaata agtcctcaat atcgtcaccc gtatatagaa
    13741 ttggattatc ctccaatgat tctttaggat ttagcctcaa atgattcgtt attttaccta
    13801 tagaacgagt caagaatacg cttatcaaaa gccctagaag gatcgtaaga gaaactgcga
    13861 ataaaagacc tatgataaat tgataaatat attgagtcgt ccttacataa agctctttta
    13921 tttcaaaaac agatattatc ttctcccctt caaaaggacc ctcaaacaag ccaaccagcc
    13981 tcaattcatt tttgaagtcc ctctcaaaac cagactctcc ccttctaaca aaagacaaag
    14041 tatcttcaga aagcaaactt atataaggta tctcctcctc ctcctcctcc tccagataaa
    14101 aactgcgagt attgtataaa cctttgtagc tttcaagtat agatttcctt tctgtgaagc
    14161 ccgcgactga agataatctt aaattaagct cgcttgaatt acccgacagg acagactctg
    14221 cttcttcaaa aagtatttct tgaaaagatt taaaaaccat aagagaaatc atcatagata
    14281 ttccgatggc aagaaatgtt gatgcaataa atattttgta aaaaatcttt ctacgagttt
    14341 ttttcggttt gccgaaatct ttttgaaact tggccctcac gggacttaaa aaataatcaa
    14401 aaaccaaata aagggcgatt gctataaaaa cggaaataga aacgccgttc aagacgtttt
    14461 tgatttgctc aacaaaagac aaataagcaa aatatttaag ttgaagcgtg ccgataatat
    14521 atccgacgac tgcgacgccg aatattaaaa ttgcaatctt taatggtaga ctgaaaattc
    14581 tatttaaaaa tttttctgga ttgccaatct tggtttttcc ggaaagatag cgtccgaacg
    14641 gcctaaagaa taaataagat ataaaaacaa acaatccccc gcctataatc gggacttccc
    14701 agaaataagc gaacataacc caagcaagct ggagctcgtc caaattcatg tttaaaagat
    14761 agtaaatcgg cgcgaatgac caataccacg tagaaattcc cccccacaga ataactaaag
    14821 cttgatgctc atagttaaaa tctcctcgat ttttccaaaa aaggtatata aaaaggctgt
    14881 taaaaaataa gagggccgca agccatataa gaaataaaaa tatgctaacc gttgcatatc
    14941 ccaatatgcc caataacgca tatatagttg ctgatattat gacaataata tttataagaa
    15001 gagcgatgat aatcggagct ctcattttat tattgattag gcctctttaa aagagccaga
    15061 agggcaagta atgttaaaac tctattaaac caaataagcg ggtgcgtcgt taaaattcca
    15121 gaagaataaa catagaccac gccgaagctt ccgacggcta agagaaaaat cgccccgacg
    15181 atcagagctt gttctttcct tatgaaaaaa atagtcccgg ccaagagaaa caagaccgaa
    15241 gatatcaccc tcacccaaag gagctctttc ccccatccaa aatttggatc tagcactaaa
    15301 ggcataaaat ctttaaatat aaacaaaagc caagtgacgg ccgaggagat aactacagca
    15361 agcattgaat acgaaaaaac aaaatcgtct ctcctctcgg acgctataat ttctcttttt
    15421 gcgaatgata cataaaatat ccaggcagca gaggcaagtg ttccgacttg aacaagccag
    15481 cctatagtat ctctatcatt gtgaaaaaat gttgctgcgg ctgatataga aaaaatagcc
    15541 aatattacgc atcctatggc gatattaaac ggaagagaat tgccgctcgc taaaaaaacc
    15601 ttcctttctc tatagattaa aaatgcggca atcagtccga ccgcgaaaaa gaagccggaa
    15661 agaatccaag cgctaatact tattgaacca gatggactag aagagtatgt aagctgcgga
    15721 ccgaaatatg caagacctat tcctatcaaa atcggggtga caagatattt tatgatgacg
    15781 gttaattgca tcaatattta ttataacatt aaacatacaa ctataaagtt gatatattca
    15841 cctaaaattt ttaaggagtt tgttatcttg attttaagag ttgtttttca actttctcca
    15901 gaacttcttg gatcggatgg tctgctttaa caaaatagtc cactgcgcca agcgaaatag
    15961 cttttttaat atcttcgtcc gtagcaagat ttgaaagaat aatcactgga atatttttca
    16021 gcgtaacatc ggctttaata tcttgtaata tctcaaagcc atttttattc ggcaataata
    16081 aatctaaaag aataatatcc ggtttgaaac tttgtgtaga ggcaagagct gcgccgccat
    16141 cgcttaccac acggacgtca taattcttct tctcaaattt agcccttaaa gcctctgcca
    16201 aagtaatctc gtcctctacg acaagtattt tcatatctgc cattataacc tattcctccg
    16261 ttaccgcaat attatttgcc tgcctgtttc ctgattttag caattatgtc ttctgctttt
    16321 ttcttctcaa tctctgcttt tctcttctct tccaaaagct tatcaagtat ttcttgatcg
    16381 tgtaattcgt cttctgcaag aatggttttt gttctagcct tctcagcctc tatcgcagat
    16441 ctagctttgc tacgctcttt tagaagggcg gatgcagctc gtttcttcgc cgagacaagg
    16501 gcctctctag ccttctttcg gactgactct agaatctctt ccgttttttt gatgcgcaga
    16561 agcgcggctt tagctttctt tctttctata agcaagttct tcttgctttt ttcttcctcg
    16621 gttctcattc tcgtttcttc tttttcttct tttctagaaa tagcttcttt ggctttcttt
    16681 atctcctctc ttaatttctt agctttttgc tgtttgagtt cgcgcagttc acggttaact
    16741 ttattcattt ctctcagagt tttctgtttt ttctggattt cttcttttaa aatgaccctc
    16801 atttttcttc tctctgctct aagcttctca gcttctcttt tcctttcgtt tatcatcacc
    16861 tctcgatcgc gtttctcatc ctcttttaga atgactgccg ttttatcttt ctcatgagct
    16921 atcgccatct tcaccttcat tttttctcct gctaaagttg acctgctttt ttgcttatga
    16981 acggcaagcg cctcagccct actccttgcc tcgtgtctgg ccttttttat gtctctggct
    17041 aggtcccttt ttgccccaag catcttttgt ttttcagatc tttctttagc ctgcagatct
    17101 tttatctttt tattaagaat gattatagat gatgctgttt cttttttagc tgtcgcgatt
    17161 cctttagctt cttcgtcttt ttcagatatg cgcaaatcag cgtcattttt tattttaaca
    17221 atttcttttc tgaatttttc ccgttcggct aaaataaggt ttctgcctct ttctcgttcg
    17281 gcttttattc tataagactc ttttcctttt tcaacaagca ttgcttctct agcccgtttt
    17341 ttctcatctc taatggccaa ccttgccttt tctctttcag tcttaatttg ttctgcttgt
    17401 ttcaacctta gagaggatag tgcagactta tatttcgcgc tttctttaat ggcagtttta
    17461 gccctttttc tttcttctaa aagcgccgtg tctctaaatt cgcgttccag atgagttgat
    17521 tctgcgatct tctcttcagc tgcaagagct gac
//
LOCUS       DATDNZ010000024         8815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2941350, whole genome shotgun sequence.
ACCESSION   DATDNZ010000024 DATDNZ010000000
VERSION     DATDNZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941350"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VI432_01010"
     CDS             complement(<1..997)
                     /locus_tag="VI432_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4496713.1"
                     /translation="MDRLKIEISWTSLWRIIIMATFVILIYVLRDVVAIVLLAIFIST
                     ALGGVVARLERYKVPRILGTIIVFLGLIILMAFILYTVLPIAIVQLNSLFSNLSEASD
                     DFFGSDFALQIRELINPDLNNLANVLLSGSASFIDIVGKFLGGAAYVLTVLVLAFYLT
                     VGRDGVKEFIRAVFPNNLEEKVVDLYVRTSRKIGRWFYAQLLLSIIVGISVFIGLSIL
                     GVKYALVLGIFSGVFELIPVVGPIFAGAIAIIIAATDSFSLGIYVLLLFIIIQQIENN
                     FLVPFLLRKAIDTHPVIILVAILGGLKLAGLVGMILALPAAVLGQELINDWVAKKS"
     gene            complement(1008..2279)
                     /locus_tag="VI432_01015"
     CDS             complement(1008..2279)
                     /locus_tag="VI432_01015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4496714.1"
                     /translation="MNFRKTVLENGLRIITVPQKESRSVTVLVLVEAGSEYETKDING
                     VSHFLEHMCFKGTKRRPTTLVISSELDNLGASYNAFTSFEYTGYYAKVESKHLNKILD
                     IVSDLYLNPLFDPQEIDKERGVVIEEMNMFEDNPMERAPELFVELMYGDQPAGWNVAG
                     TKEVINKLKREDFIKYRSDHYVSSATTIVVAGSFEEMSTIENIKGIFSGMDTSAKVGK
                     SKTVEVQHEPQLLVKTKGLDQIHIVLGFRAFDIFDDRRYALEVLATILGGGMSSRLYK
                     RVREELGAAYYIRAGSQAHIDHGYLAIESGINRDKLSSVLDAIIDEVRKIKIEKVSNE
                     ELIRAKNQLSARLNMALETSNSLAEFYGIQDIVERRILTPEEILERINSVSPNDILDI
                     AKIIFTNNKLNLVVLGPEQDEKALRQRLSLD"
     gene            complement(2282..3646)
                     /locus_tag="VI432_01020"
     CDS             complement(2282..3646)
                     /locus_tag="VI432_01020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY4496715.1"
                     /translation="MANTDLMEKVVSLCKRRGFIFPGSEIYGGLANSWDYGPLGVELK
                     NNIKNLWWKKFVHERGDMVGIDAALIMNPKVWEASGHLSNFNDPLVECKNCNSRFRLD
                     HLLEQSGKMDVDGVSFEEMAKKIKSGSDIVCLNCGKRNWGDAKQFNMMLRTYLGPTEE
                     EGSEVYFRPETAQAMFVDFKNVVSSSRVKLPFGIAQIGKAFRNEITPGNFIFRTREFE
                     QMEIEYFFNPKDDWPAHFDYWRDEMIDWMKEIGLDTNKIHQFDVPDGERAHYSKRTID
                     FEYDFPFGKDELYGLAYRTDFDLKNHFKDKPPYRDPDSGEEIYPHVIEPTFGVDRTIL
                     ALLVDSYYEDGDRVVLKLNNNLAPYKAAIFPLLANKPQLVHLAREIFDSLKKDINVVW
                     DDRGNIGKRYYSQDEIGTPYCITVDFDSLEDKSVTVRDRDTAKQERIKIKKLDSWLQD
                     KIKI"
     gene            complement(3683..5425)
                     /locus_tag="VI432_01025"
     CDS             complement(3683..5425)
                     /locus_tag="VI432_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496716.1"
                     /translation="MRSKYKRKKRKNSFIVFISGAILLVVILVFAIIVYLNGGSKGIN
                     LNILRDAEEIRAGVPFDLTVELDNLAKNNLQDVVISLNLPDGSAVLGEEDKKIVNREI
                     QSIGEGIMHKEIFRVIILPGGESDFRIEATTFYSIGDLPARFERKMGHQIKIAPSLIS
                     LNLDAPEGVISGAEFDIGIGYFGDENQNFESLRLEFEYPDEFTIISTSRPSDIRNNSW
                     YIDELPIEESDDLIIKGRVDLPDGSVFSLTAKLIARFLGREYVIASSSREVAITESPL
                     SVDILTDNNSGFYSSGEDIVYTINYKNNTDIDLKNIKVSAKLSGSMFDWRSLITDGSF
                     NQFTNTISWDALSIEHLNNLAPGEINGLNFSIRLLDDQPIKKLNDRNFTIKVETRIES
                     PTVPDGISASKISNSNTWEGKIIGKILIDGQGFFRDAESLILNEGPWPPKVGQPTDFT
                     IHLYITNFSTDVRDVEIRTKLEPGVQMTDKFRSNTSFLPEFDVLTGEVVWKLDKLIAT
                     SGITGEKPSVIFQVRATPSLNFLGQPMPLVSGVTIKALDEFTGQELNSSHEAITTSSL
                     DDLTVKPEEGVVIQ"
     gene            complement(5409..5966)
                     /gene="recO"
                     /locus_tag="VI432_01030"
     CDS             complement(5409..5966)
                     /gene="recO"
                     /locus_tag="VI432_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY4496717.1"
                     /translation="MTEYFTKGLVLSRDDRGEFDGVFSIYTKDFGKIRATARSVRRIK
                     SKLSPHLSIGNFADLRIIKNNNYQIVDAFSFDKSKLNQRKLKFIEFIEQMTPDESQDH
                     HLWYGIDYVLSNNLIGKGGSDNDEFEHKIYRRFLEIFGFGAKFARCGNCGSKDIVYFI
                     PPDIMFLCLKSSRFLAIKEEDAIKI"
     gene            6095..6547
                     /locus_tag="VI432_01035"
     CDS             6095..6547
                     /locus_tag="VI432_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496718.1"
                     /translation="MRKTLKYIAIAIVIGILLSIPQTNKTEGEEDYHLEYGVYNGFEG
                     AAIVNSTTIFPIERIVKVTAYSSREQETDSTPFITASGTRVRDGIVAANWLPIGTKVR
                     IPEAFGDKIFTVEDRMHKRNSDKLDIWFASTKEAFKFGVQELRVEILL"
     gene            complement(6551..7885)
                     /locus_tag="VI432_01040"
     CDS             complement(6551..7885)
                     /locus_tag="VI432_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496719.1"
                     /translation="MDEKLSLYIKEQRDRGISDDAIKRTLLGVGWSEADVSYAMNSSE
                     VNTPASLVAEAPKSGINLKVVAAVIVGFLILVGGAYAYYEFQNESPEDIIVQAITNLA
                     KAKSFDYNVQILVPLDMDSAESFLNSLSEPSEEFINEFRQRSEELGLVPGIDGASEQE
                     ILANYPLATVISDISGSLDKRDDSNIKSKLDIRLSLDFLRPSNLTEEILYLQTLFANK
                     KFYIKISLPSDNFQPETGFVPSGIAKQIADKWIVIDLESIKELSESFNPEIKDNIENF
                     ENNESSDKFDKYRDEIISSFKNSKVFSVTEENGTSDRYIYTLVLDKDGIKRFITEAYS
                     ILEKVEEDLKSNLNVPPKSALDDMINEIDNLPEESIPSFKIEIVKDGIFLAKIEPASA
                     NNRSLGSISFNNFNGDIQINSPADSLDIKELMQIILPMLFFGGSEFEFAPQL"
     gene            complement(7975..8637)
                     /locus_tag="VI432_01045"
     CDS             complement(7975..8637)
                     /locus_tag="VI432_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496720.1"
                     /translation="MEQVLSKRRLLWVHDGNPARPHALLSSGKHGTGFANIREALTDP
                     ILREKTALTIIRGLEKYINPKEVAVVAGSGYASIFLVGDVARILSYRFGQTVRSVFTD
                     KVDPNGKEMEMKFTLEPGEVIFLVEDMQTTFHTTLSTIDAIKRAQPEATIISYTACAF
                     AWMSVLSYLGENNFTVNPILMRDMTVLSPEVCSDVGLCGKGSEAIKPKARGNWVRLTA
                     NY"
BASE COUNT         2745 a         2007 c         1380 g         2683 t
ORIGIN      
        1 gagatttctt agctacccaa tcattaataa gctcctgtcc taaaacggcg gctggaagag
       61 ccaaaatcat accaactaaa ccagctaatt ttagcccgcc taaaattgcg actaagataa
      121 tcaccggatg agtatcaatc gccttacgca aaaggaatgg aacaagaaaa ttattttcta
      181 tttgctgaat aatgataaaa agcaataaaa catatattcc cagagaaaaa gaatccgtcg
      241 cggcgattat aatcgcgatt gcgccagcaa aaattggccc cacaaccgga ataagctcga
      301 aaaccccaga gaaaatcccc aagactaaag catacttaac ccccaagata gaaagtccta
      361 taaataccga tattcctaca ataatactta ataacaattg agcataaaac catcgtccaa
      421 ttttacgcga tgttctcacg tataaatcaa ctactttctc ttctaaatta ttagggaaca
      481 cggctcttat aaactccttt actccgtcgc ggccaaccgt cagataaaaa gctaaaacca
      541 aaacagtcaa aacatacgcc gctcccccta aaaacttgcc gactatgtca ataaaagaag
      601 cgctaccaga aagcaaaaca ttcgcaaggt tattcaaatc aggatttatt aattccctaa
      661 tctgcaaagc aaaatccgaa ccaaaaaagt cgtcagatgc ttcacttaaa ttgctaaaaa
      721 ggctattcaa ttggactatg gctatcggca gaacagtata gaggataaaa gccatcaata
      781 ttatcaaacc taaaaataca ataattgttc ccaatattct gggaacctta tatctttcca
      841 agcgagccac cactccacct aaagcagtgg atatgaaaat agccagcaac acaatagcta
      901 cgacatctcg aagcacataa ataaggatca cgaaagtcgc cataattatg atcctccaaa
      961 gactcgtcca agatatttca atcttaagcc tgtccattaa ccttattcta atctaacgag
     1021 agcctttgac gcaatgcttt ctcatcttgc tccggcccca aaaccactaa atttaatttg
     1081 ttgttcgtga aaatgatttt tgctatatct aatatatcat tagggcttac agaattaatt
     1141 ctctccaaaa tttcctctgg agtaagtatt cgtctctcaa cgatatcttg aatgccataa
     1201 aactcagcca ggctattaga ggtttctaaa gccatgttta atcttgcaga caactgattt
     1261 tttgccctaa ttaattcctc attgctaact ttttctattt tgatttttcg cacttcgtca
     1321 atgatggcat cgagaactga actaagctta tctctattta tacccgattc aatggctaaa
     1381 taaccatgat caatatgagc ctgagagcca gcacggatat aatacgcggc acccaattct
     1441 tctctgaccc tcttgtaaag tctcgagctc atgcctcctc ctaatattgt agccaacacc
     1501 tccaacgcat aacgcctatc atcaaatata tcaaaagccc taaatcctaa aactatatga
     1561 atttgatcta gtccctttgt tttaactaac aattgaggtt catgctggac ttcaacagtt
     1621 tttgatttcc caaccttagc actggtatcc ataccagaga agattccttt tatattctca
     1681 atggtagaca tttcctcaaa cgaaccagcg acaacgatgg tggttgccga agaaacataa
     1741 tgatctgacc tgtatttgat aaaatcctcc ctcttcagct tattaatcac ctcttttgtg
     1801 ccggcaacat tccacccagc cggctggtct ccatacatca actcaacaaa taattctggg
     1861 gctctttcca tcggattgtc ttcaaacata ttcatctctt caataaccac gcccctttct
     1921 ttatctattt cttgagggtc aaataatgga ttcaaatata aatcagaaac tatatctaag
     1981 attttattta aatgttttga ttcaaccttg gcataataac cagtatattc aaaactcgtg
     2041 aatgcattat atgaggcgcc tagattatct aattctgatg atataacaag agtagtgggc
     2101 cgcctttttg tgcccttaaa acacatatgc tccaagaagt gagaaacgcc gtttatatct
     2161 tttgtctcat attcggaacc ggcctcaact aaaaccagaa cagtcacaga tctgctttct
     2221 ttctgaggca cggttataat acgcaaaccg ttttccaaaa ccgtttttct aaaattcata
     2281 cttatatttt tatcttatcc tgaagccagg agtctaattt tttaattttt attctctctt
     2341 gcttagcagt atctctatcg cgcacagtga ctgatttatc ctctaaacta tcgaagtcta
     2401 cagttataca atacggcgtg ccgatttcat cctgcgaata ataacgtttt ccaatattac
     2461 ctctatcatc ccaaaccaca ttaatatcct tttttaatga atcaaagatt tctctagcta
     2521 aatggacaag ctgcggctta ttggcaagta atggaaagat ggccgctttg tatggcgcta
     2581 aattattatt cagtttcaaa acaactctgt cgccatcttc gtaataacta tcaactaaaa
     2641 gagccaatat agtcctgtct actccaaatg taggttctat cacatgagga tagatctctt
     2701 cacctgagtc gggatcgcga taaggcggtt tatccttgaa atgatttttc aaatcaaagt
     2761 ctgttcgata cgcaaggcca tatagttcgt ctttgccgaa cggaaagtca tattcaaaat
     2821 caattgtgcg cttagaataa tgcgctcttt ccccatcagg gacatcaaac tgatgaattt
     2881 tattcgtgtc taagcctatt tccttcatcc aatcgatcat ctcgtctctc caataatcaa
     2941 aatgcgcagg ccagtcgtct tttggattaa aaaaatactc aatctccatt tgttcaaact
     3001 cacgagttct aaagataaaa tttccgggcg ttatttcatt tctgaaagct ttgccgattt
     3061 gagcaatccc aaaaggcaac ttgactcggc ttgatgaaac aacgtttttg aaatccacaa
     3121 acatggcttg cgcagtttcc ggacggaaat aaacttcact accctcctcc tctgttggac
     3181 ccagataagt tctaagcatc atattaaatt gcttggcatc accccaattc ctcttaccac
     3241 aatttagaca aacgatgtct gaaccactct ttattttttt agccatctcc tcaaaactca
     3301 caccatcaac atccattttt ccggattgtt ccaataggtg atctagacga aaacggctat
     3361 tgcaattttt acactcaaca agcggatcgt tgaaattaga aagatgtccg ctcgcctccc
     3421 aaacctttgg gttcattatc aaagcagcgt caattccaac catatcgcct cgttcgtgaa
     3481 caaacttttt ccaccacaga tttttaatat tattcttcag ctctacccct aaagggccgt
     3541 agtcccacga attagccagt cccccgtaaa tttcgctgcc cgggaaaata aaaccgcgcc
     3601 tcttgcaaag cgaaaccact ttttccatca aatcagtatt tgccatttgt tgccgattaa
     3661 cttttaaatg ttaattgtct ttttattgta tcacaacgcc ttcttccggc ttgaccgtca
     3721 gatcgtctaa agagcttgtt gtaattgcct cgtgagaaga attaagttct tgccccgtaa
     3781 actcatctaa agccttaatc gttacaccgg atacaagtgg catcggctga cccagaaaat
     3841 taagactcgg ggttgctcta acctgaaata taacagacgg cttctctccg gttataccac
     3901 tcgtcgcgat aagcttatcc aacttccaca caacctcgcc cgttaaaaca tcgaactccg
     3961 gcaaaaaact ggtattactt ctaaacttgt ctgtcatctg aacgccgggc tcaagctttg
     4021 ttctgatctc tacgtctctc acatccgtac taaaatttgt tatatacaga tggatcgtaa
     4081 aatcagttgg ctggccgact tttggaggcc acggaccctc atttaaaatc aatgattcgg
     4141 catccctaaa aaaaccttgg ccgtcgatta atatctttcc aatgattttc ccttcccaag
     4201 tattggaatt actaattttt gaagcggaaa ttccatctgg cacagtagga gattcgatgc
     4261 gtgtttcaac cttaatcgta aaattcctat catttaactt cttaatcggc tgatcatcta
     4321 aaagtcttat cgaaaaattc aagccgttta tctctcctgg ggctaaatta tttaaatgtt
     4381 caattgataa tgcgtcccaa ctaatagtat ttgtaaattg attaaacgat ccgtcggtta
     4441 ttaaactcct ccagtcaaac atagaacccg acaattttgc gcttactttt atatttttta
     4501 aatcaatgtc ggtattattt ttataattta tagtgtatac aatatcctcg cccgatgaat
     4561 aaaaaccaga attattatcg gtcaaaatat ctactgaaag aggagactca gttatagcga
     4621 cctctctaga agaggatgca ataacatatt ccctgcctaa aaacctggca attaatttag
     4681 ctgttaagct aaataccgat ccatccggga gatcgactct tcctttaata attagatcgt
     4741 ctgattcttc tataggcagt tcatctatat accatgaatt attgcgtata tccgatgggc
     4801 ggcttgtaga gattattgta aattcatccg gatattcaaa ttcaagtctc aaggattcaa
     4861 aattctgatt ttcatcaccg aaatatccta ttcctatgtc aaactctgct cctgatataa
     4921 ccccttccgg agcatcaaga ttaagactta taagagatgg cgcaatttta atctggtggc
     4981 ccatctttct ctcaaacctt gccggaagat ctccgataga ataaaaagtg gtcgcctcaa
     5041 tcctgaaatc agattctcct ccgggcaata ttattaccct aaaaatttct ttatgcataa
     5101 tgccttctcc aatactctgt atttctctgt taacaatttt cttatcctcc tcgcccaaaa
     5161 cagccgaacc atctggcaaa tttaaagaaa tgacaacatc ctgaagatta tttttggcta
     5221 aattatccaa ttcaaccgtt aaatcaaatg gaactccagc tcttatttct tcagcatccc
     5281 ttaatatgtt tagatttatt cctttagatc ctccgtttaa atatacgatt atagcgaata
     5341 ccaaaattac aaccaaaagt atcgcgcccg atataaaaac tataaaacta ttttttcttt
     5401 tctttctttt atattttgat cgcatcttct tctttaatag ccaggaacct tgaggatttc
     5461 aagcataaaa acattatatc cgggggtatg aaataaacaa tgtctttact cccgcaatta
     5521 ccgcatctag cgaatttggc gccaaacccg aaaatctcta aaaatctacg ataaatttta
     5581 tgttcaaatt catcattgtc actaccccct tttcctatca ggttattaga tagaacgtaa
     5641 tctatgccgt accataagtg atgatcttgc gattcatctg gagtcatctg ctcaataaac
     5701 tcaataaatt tcagttttct ttgattgagt tttgatttat caaaggaaaa ggcatcaaca
     5761 atctgataat tattattctt gattatcctc agatccgcaa aattaccgat agagagatga
     5821 ggcgataatt ttgattttat cctgcggacg cttctggctg tagcccttat ctttccgaaa
     5881 tctttagtat aaatactaaa aacaccgtca aattctcccc tatcgtccct cgataacacc
     5941 aaaccctttg taaaatattc agtcattgaa atagattata caaatatttc agctatttag
     6001 cataactcaa aaatcgtttg acacgaattg cccatactgt taatatggct tgcatcataa
     6061 atactccccg gaaggggaga tttttattag caatatgaga aaaacattga aatatatagc
     6121 aatcgcgata gtaataggca ttttattgtc tatcccccaa accaataaga cagaaggaga
     6181 agaagattat catttagaat acggcgtata taatggtttt gagggagcgg caatagtgaa
     6241 ttcaaccaca atattcccca tcgagcggat tgttaaagta acagcatatt cgagccgaga
     6301 gcaagaaacg gacagtacgc cgtttataac cgcctccggc acgagggtac gcgatggaat
     6361 tgttgcagcc aactggctgc caatcggaac gaaagtcaga attcctgaag ctttcggaga
     6421 taaaattttc actgttgaag accgaatgca caaacgtaac tccgacaaat tggatatatg
     6481 gtttgcaagc acaaaagaag cttttaaatt cggcgtccaa gaattaaggg tagaaatcct
     6541 attatagtaa ctataactgt ggagcaaact caaactctga tccgccgaaa aacaacatcg
     6601 gcagaataat ctgcatcaac tcttttatat ccaagctatc agccggagaa ttaatttgga
     6661 tgtctccatt aaaattatta aaggatattg accccaaaga cctattatta gccgatgccg
     6721 gttctatctt tgccaaaaat atgccgtctt tcacaatctc tattttaaaa cttggaatac
     6781 tctcttctgg taaattatct atttcattta tcatatcatc cagcgcgctt ttaggcggaa
     6841 catttaaatt acttttcaag tcttcttcta ctttttccaa gatagaataa gcttctgtaa
     6901 taaatctttt aattccatcc ttatctaaaa ccaaggtgta aatataccga tcactagttc
     6961 cattttcttc ggttacgcta aagaccttgc tgtttttaaa agatgatatt atttcatctc
     7021 tatatttatc aaatttatcg gaagattcat tattttcaaa attctctata ttatccttta
     7081 tctccgggtt aaagctctct gataactctt taatactctc taaatctata actatccact
     7141 tatcagcaat ctgttttgca atcccagatg ggacaaatcc tgtctccggc tgaaagttat
     7201 cggaaggaag agatattttt atataaaatt ttttattcgc aaacaacgtc tgtaaatata
     7261 gaatttcttc ggttaaattg cttggcctta gaaaatctaa cgacagcctt atgtccagct
     7321 tagatttaat attgctgtca tctctcttgt ctaaagatcc gctgatatca gatataacgg
     7381 ttgcgagcgg ataattagca aggatctctt gttctgacgc tccatcaata cccggcacta
     7441 aaccgagttc ttcgctcctt tgtctgaatt catttataaa ctcttctgac ggctcgctca
     7501 atgaatttaa aaaactctct gctgaatcca tatcaagcgg aacgagaatc tgaacgttat
     7561 aatcaaatga tttagcttta gctaaattag taattgcctg cacaataatg tcttcaggcg
     7621 attcattttg gaattcataa tacgcatatg cgcctccaac aagaattaaa aatcctacga
     7681 tgacggccgc gacaactttt aagttaatgc cggactttgg cgcttcagca accaaagacg
     7741 cgggagtgtt cacttcagaa gaattcatag cataagaaac atcagcctcg ctccacccaa
     7801 cacccaaaag ggttctttta atcgcgtcat cactaatacc tctatctctt tgttctttta
     7861 tatataaact aagcttttcg tccattttaa aagcttaaca ccatacacat tgattatcta
     7921 tacacaatag aaaaagcagc catatattta tggctgcaag aatgctcctt cccgctaata
     7981 atttgctgtt agtctcaccc agtttccccg agctttaggc ttgatggcct ctgacccttt
     8041 tccgcaaaga cccacatcac tacacacctc cggcgataaa accgtcatat ctctcatgag
     8101 aatcggatta acggtaaagt tattttcgcc aagataagag agaacactca tccaagcaaa
     8161 agcgcatgca gtataagata taatcgttgc ctctggttgg gctcttttaa tggcatcgat
     8221 tgtggataac gttgtgtgaa aagtagtctg catgtcttca acaaggaaaa taacttctcc
     8281 aggctcaaga gtaaacttca tttccatctc cttaccgttc ggatcaacct tgtcggtaaa
     8341 cacgctacga accgtctggc caaaacgata agaaaggata cgcgcgacat cgccaaccaa
     8401 aaaaattgac gcatagccag aaccagctac cacagcaacc tctttcgggt ttatatattt
     8461 ctccaaccca cgaataattg tcagggctgt tttttcgcgc aaaattggat ctgtcaacgc
     8521 ttcacggata tttgcaaatc ctgttccatg ttttccactt gataaaagcg catgcggacg
     8581 cgcgggattt ccatcgtgga cccaaagcaa tcttcttttt gaaagaactt gttccaactc
     8641 ttctgaggta attagttcga gtatccttat acctccttac aaaacccatt gggtttgcct
     8701 aaaaactaaa ctaataagga agaaaaggca aatttatttg tgcgcgggag aggatttgaa
     8761 cctccacgcc cgaatcgggc gctaccacct caaggtagtg cgtctgccgt ttcgc
//
LOCUS       DATDNZ010000025         4932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2173385, whole genome shotgun sequence.
ACCESSION   DATDNZ010000025 DATDNZ010000000
VERSION     DATDNZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173385"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="secD"
                     /locus_tag="VI432_01050"
     CDS             <1..1068
                     /gene="secD"
                     /locus_tag="VI432_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY4496721.1"
                     /translation="GLRDVIEKRVNLFGVAEPKVVTAYKDGSYQLLIELAGVKELSDA
                     VKQIGEVPVLDFREVQISGETATIIPTDLTGRYIKNASIGLDNITNRAVVNFELDKGG
                     AEIFERITEKNVGKPICIFVDGNFIFPEDQPASCPNVNEKISGGKAQISGGNITIDSA
                     RQLVSRFQAGALSAPIKPINQRTVSSSVAEDSLEKILIAGAAGTFLVLIFMIVYYKLF
                     GFFSAIALMIYTVLTLSVFKLIPDFTMTLAGIAGFILSIGMAVDANILIFERTKEEIK
                     RGLSRRSAIEEGFKRAWSSIRDSNISTIITAIILYYFTSSFVKGFALTLGVGVLMSMF
                     SAIFVTRVMLRIFIKNKHERN"
     gene            1055..1948
                     /gene="secF"
                     /locus_tag="VI432_01055"
     CDS             1055..1948
                     /gene="secF"
                     /locus_tag="VI432_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY4496722.1"
                     /translation="MNVIKYKNIFIVISFIAVAASVVSIAYFGFNLGIDFTSGSLWQI
                     KVLGSNPAEIRSFFYSELETELSSVSYDKDNEIYTISFAEITDETRQNMFSVIKEKFG
                     DDVEDLDFWTISPTVSKELTRKAILAIGFVLLAISLYIAFAFRKISKPISSWKYGFIT
                     LITLMHDVAIPAGLFAYLGYQFGASVDTNFIVALLVVMGFSVHDTIVVFDRVKENLTK
                     TLGKKSLGDIINTSIKETIHRSINTSATLILVLITLFFFGPSSLQFFILTILVGTIIG
                     TYSSIFLASPMLTFLEGKHKS"
     gene            1994..2821
                     /locus_tag="VI432_01060"
     CDS             1994..2821
                     /locus_tag="VI432_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY4496723.1"
                     /translation="MDLQKLDNLIDEVVGSLNQRSGQIITHRYGLSGEDKKTLAELGE
                     RYDLTRERIRQIESQTLNAIRDEIKTHKEVIRFLKFIHAYLNKIGNVRPSKLLAEDIL
                     ADSDNGKDSPALINRLHFLADALDEPNISYGDDEWHDVWHNDESAYKTAQKVVSYLTS
                     FKEHDFDKFIKDAVEKFGLPDEIILNYLHSSKNFGKGPYGDYGAMHWIHVNPRTARDK
                     SYLVLKKSGAPLHFREIAGRVNKLDGVKTSHPDTIHNELIKDPRFVLVGRGTYGLRD"
     gene            2821..4233
                     /locus_tag="VI432_01065"
     CDS             2821..4233
                     /locus_tag="VI432_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496724.1"
                     /translation="MTLLAELFSIVEKFVLEYWTLFLGIVFIVYGTYIAFVLARRAYL
                     MKLHNKTFADLEKDMITLSLKFPKENLRSPQAMEQVFASMQGYYSFGFNFKLKWILGE
                     IEKWTSLEIVGNAGSVKFYARIHKKLRRLLENGLFAQYPEIEIREVPDYFESLPEELP
                     NDKFDLWGTDLVLARENHYPIRTYVEYDSPDEEQRLDPISMITEVMANLKDDEWMMLH
                     VLFRPTGKDFFPNYEKDAQKQINKISGKKEPPGKPGFISIFLDFFLNLIKALNPWDAS
                     ELQWGGGKKEEKMEFSKFPNPAEVEEMKAIDRKLSKIPFECIVRWLYIDNREDITSSN
                     VFNLMGSVKQWDTKNFNSLRPSGATFTYLTKVGLFAHDTSSFFRKYINKSDIFFRGKR
                     LAKRKKKVYQLFRHRIFPANTQPMTYHKAGSIYLKSYILNAEELASIFHPPGFPVKAQ
                     KLEQIYTRKGGPPDELPIIE"
     gene            4241..>4932
                     /locus_tag="VI432_01070"
     CDS             4241..>4932
                     /locus_tag="VI432_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY4496725.1"
                     /translation="MDNQEKPTLLGVTNFRDKKVKFGIKKDDRRRHFYIIGKTGGGKT
                     TMLENMIVSDIQAGNGVGVIDPHGDFAEKLLKYVPESRIDDVIYFNPADENFPFAFNP
                     LERTGQEHRHLVASGLMSVFKKIWPDVWSARMEYILNNAILTLLEYPGSTLLGIMRIL
                     SDKEYRARAVSKLTDPVIRNFWVNEFSRYTQRLETEATAAIQNKVGQFISNPLIRNIV
                     GQPKSTLNFREI"
BASE COUNT         1559 a          804 c         1053 g         1516 t
ORIGIN      
        1 gggcttcgcg acgttattga aaaaagagtt aatttatttg gcgttgccga gccaaaggtt
       61 gtaactgctt ataaagacgg gtcatatcaa ttattgatag aattagccgg tgttaaagag
      121 ctaagcgatg ccgttaagca gatcggagaa gttccggtgc ttgattttag ggaggttcaa
      181 ataagcggtg agacggcgac aataattcct acggatttaa ctgggcgtta tattaaaaac
      241 gccagcatag gattagataa tattacgaat agggctgtcg ttaatttcga actagataaa
      301 gggggggcag agatttttga aagaatcaca gaaaaaaatg tcggcaaacc gatctgtatt
      361 tttgtagatg gtaattttat ttttcccgaa gatcagccgg ccagctgtcc gaatgtgaac
      421 gagaaaatat ctggaggcaa agcgcagata tctggcggaa atataaccat tgattcggct
      481 aggcaattgg taagcagatt ccaggcaggc gcgttatcgg ctccaataaa accgattaac
      541 caaagaacgg ttagctcatc ggtggctgaa gactcgcttg agaagatctt gatcgctggg
      601 gctgctggta cttttctggt tttaatattc atgattgtct actataagct atttggtttt
      661 ttctctgcta tagcgttgat gatctatacc gttttgactc tttctgtttt taaacttatt
      721 ccggatttca caatgactct ggccggaata gccggtttta ttctatcgat tggaatggcg
      781 gttgatgcta atattttgat ttttgaaaga actaaagaag aaattaaaag aggattgagt
      841 cgcagaagcg ctattgaaga gggttttaag cgagcatggt cttctatccg tgattcgaat
      901 ataagcacga tcatcacagc tattattctt tattatttta catccagttt cgttaagggt
      961 tttgctttga ctttgggggt cggagtccta atgagcatgt tctcggcaat atttgtgacg
     1021 agagtgatgt taagaatttt tatcaaaaat aaacatgaac gtaattaaat ataaaaatat
     1081 ttttatcgtt atatcattta ttgcagttgc ggcatccgta gtaagcattg cttatttcgg
     1141 attcaattta ggcatagatt ttaccagcgg ttctctatgg caaattaaag ttttgggaag
     1201 caatccggct gagattaggt cgtttttcta ttctgaactt gagactgaat tatctagcgt
     1261 ttcttatgac aaagataatg aaatatatac gatttctttt gccgaaatta cagacgagac
     1321 aagacaaaat atgttctctg tcataaaaga aaagtttggc gatgacgttg aagatctgga
     1381 tttctggaca ataagtccaa ctgtatctaa agaattaaca aggaaagcga ttcttgctat
     1441 agggtttgtt cttcttgcta tttctttgta tatagcgttt gcttttagaa agatatctaa
     1501 accgataagc tcttggaagt atggatttat aactttaata actttgatgc atgacgttgc
     1561 tattccagcc ggactttttg cttatttggg atatcaattc ggagcctcgg ttgacactaa
     1621 ttttatcgtt gcccttctag tcgttatggg cttttccgtg cacgatacaa ttgttgtttt
     1681 tgacagggtt aaagaaaatc tcaccaaaac attgggcaag aagagtcttg gagatattat
     1741 aaatacaagt ataaaagaaa ctattcatag atctattaat acctcggcaa ccttaatctt
     1801 agttttaata actctgtttt tcttcggtcc atcaagcctt caattcttta ttttaacaat
     1861 actcgtcgga acgattatcg gaacatactc ctcaatcttt ttagctagcc caatgctcac
     1921 ctttttagag ggtaagcata agtcttgaca gcgggtcaac taaattgtat tatatagcaa
     1981 attgaaaata agcatggatt tacaaaaatt agacaattta atagacgagg tggttggatc
     2041 gttaaatcag cgttctggcc agattattac tcatagatat ggtctttctg gtgaagataa
     2101 gaaaacccta gccgaactgg gagagagata tgaccttact cgtgaaagaa taaggcaaat
     2161 tgagtcgcaa accctcaacg ctattagaga tgagattaag actcataaag aggttattag
     2221 gtttttgaag tttatacacg cctatttaaa taagataggc aatgttagac caagcaagct
     2281 tttggcagaa gatattttag ctgatagtga taatggaaaa gacagccccg ctttaattaa
     2341 tagacttcat tttttagctg atgcacttga tgagcctaat atctcgtatg gagatgatga
     2401 atggcacgat gtgtggcaca acgatgaatc ggcttataaa acagcccaga aagtggtttc
     2461 ttatttaacc agctttaaag aacacgattt tgataaattt attaaggatg cggttgaaaa
     2521 attcggatta ccggatgaaa taatattaaa ttatctacat tcctcaaaaa atttcggcaa
     2581 gggtccatat ggtgactatg gagcaatgca ctggattcat gttaatcctc gtacagccag
     2641 agataaaagc tatcttgttt taaagaaatc tggagctccg ttgcacttta gagaaatagc
     2701 tggaagggtc aataagcttg atggcgtaaa gacctctcat cctgatacaa tacataatga
     2761 gcttattaag gatccgagat ttgtcttggt tggccgtggc acttatgggc tcagagacta
     2821 atgactttac tcgcggaact tttttctatt gttgaaaaat ttgttttaga atattggacc
     2881 ctttttctcg ggattgtttt tatagtttac ggaacataca tagcttttgt tttggcaaga
     2941 agagcttatt taatgaagct tcataataag actttcgctg atcttgagaa agacatgatt
     3001 actttatctc tcaagtttcc taaggagaac ttaagatctc cccaggcaat ggagcaggtt
     3061 tttgcaagca tgcagggtta ctacagtttt ggttttaact ttaaactcaa gtggattttg
     3121 ggagaaatag agaaatggac atcgcttgaa atcgtgggca atgccgggtc tgttaagttt
     3181 tatgctcgga tacacaagaa attaagaaga cttttggaaa atgggctatt tgcccagtat
     3241 ccagaaatcg agataagaga agtgccggac tattttgaaa gtctgccgga agaattgcct
     3301 aacgataaat tcgatctttg gggaaccgac ttggttctgg cgagagagaa tcattatcca
     3361 ataagaactt atgttgaata tgattctcct gacgaagagc agcgactaga tccgatctct
     3421 atgataacgg aggttatggc gaatcttaaa gacgatgaat ggatgatgct ccatgtttta
     3481 ttcagaccga ctggtaagga ttttttccca aattatgaaa aagacgctca aaaacaaatt
     3541 aataaaatat ctgggaagaa agagccgcct ggtaaacctg gtttcatttc tatcttttta
     3601 gatttctttt taaatcttat aaaggctctt aatccttggg atgcgtctga gttgcaatgg
     3661 ggcggcggca agaaagaaga aaaaatggag ttttctaaat ttccgaatcc agcagaagtg
     3721 gaagagatga aggctattga caggaagctt tcgaaaattc cttttgaatg catagtcagg
     3781 tggctatata tagacaatag agaagatatt accagcagca atgtattcaa tctgatggga
     3841 tctgttaagc aatgggatac taaaaacttt aatagtttaa ggccgagtgg cgcgactttc
     3901 acatatctta caaaagtagg attgttcgct catgatacaa gcagcttctt caggaaatat
     3961 ataaataaaa gcgatatctt ttttagaggg aaaaggctcg ccaagcgtaa gaaaaaagtt
     4021 tatcaattat tcaggcacag aatattccct gcaaatactc aaccaatgac atatcataaa
     4081 gcgggaagca tctatctaaa aagttatatt ctgaacgctg aagaattagc ttcaatcttt
     4141 cacccgccag gttttcctgt taaagctcaa aaactagaac aaatatatac taggaaaggt
     4201 ggcccgccgg atgaattgcc aattatagaa taatttcaat atggataatc aagaaaaacc
     4261 aacattattg ggcgttacga acttcagaga taagaaagtt aaattcggga tcaagaaaga
     4321 tgaccgaagg agacattttt atattattgg taaaacagga ggaggtaaaa caaccatgct
     4381 tgaaaatatg attgtaagcg acatacaagc tggaaatggt gtgggggtca ttgatccaca
     4441 cggagatttc gcggaaaaac ttcttaaata tgttccagag tctaggatcg atgatgtaat
     4501 ttatttcaat cctgcagatg agaattttcc gttcgctttc aatccgttgg aaagaaccgg
     4561 tcaggagcat agacacctgg ttgcatctgg tttaatgagc gtttttaaga agatatggcc
     4621 ggatgtttgg tctgctagaa tggaatacat tttgaataac gcaatattga ccttattaga
     4681 atatcccggt tcaacgctac ttggcattat gaggatttta tctgataaag aatatcgagc
     4741 tcgagcagtt tctaaactta ctgatccggt catccgcaat ttttgggtaa acgaattttc
     4801 gcgttacacc caaagactgg aaacggaggc tactgccgct atacagaaca aagtcggtca
     4861 attcatctct aatccgttaa tcaggaacat agtaggtcag cctaaatcaa ctttaaattt
     4921 tagagaaatt at
//
LOCUS       DATDNZ010000026         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1388528, whole genome shotgun sequence.
ACCESSION   DATDNZ010000026 DATDNZ010000000
VERSION     DATDNZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388528"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VI432_01075"
     CDS             <1..1084
                     /locus_tag="VI432_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4496726.1"
                     /translation="TDQLLDEKIKIEILLDSIGDGIIAIDRSWNITFSNEIASSISGW
                     PKKEMIGRPFRDVIKFIREYDRKENISFIENAMVLGRMQQLQEKTSLIKKNGDEILID
                     DSASPIFNDDGKVVGATIIFRDASTTQEASNLRAGFQYASHQLNTPISKIMWSLELAI
                     EEKDYDKRTKAIKEAYRSAQSVSKMSEQLFAVAQIDQDVVIPEYAEFELDVLIKDISK
                     KSENSSFRSDVKISFNIANDIKKINSDKKMLSRILQELLDNAFKFNAQDGSIDISVRL
                     IHGDIVFAIKDTGIGIVERDQALVFTKFFRGMNFDTSKISGAGLGLFISKAYTEILDG
                     KIWFTSKPGETIFSFKLPLIHLETLK"
     gene            1117..1554
                     /locus_tag="VI432_01080"
     CDS             1117..1554
                     /locus_tag="VI432_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4496727.1"
                     /translation="MERGGVEAVASVLIVKDGKILLIKSPKWDDSYLVPGGHIDRGET
                     IIDAATREGEEETGLKLKSLYCVNVGESIFDPSFYRKAHLIYFHFVCQVVGGEISPDA
                     REVREFFWFDPKEALNLKLTKGVRKTIENYINGVRIDLASAKF"
     gene            1557..2318
                     /locus_tag="VI432_01085"
     CDS             1557..2318
                     /locus_tag="VI432_01085"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4496728.1"
                     /translation="MNLSINTINNLKLSAVVHKPEGDGPFPAVILLHGFTGYKDEEHI
                     VILANDLAENGLVAVRFDASGFGESEGNTERDYRMSNHLENAESAYKFLIEQDYVDEK
                     RVGVWGHSMGGGLAIIFASKHPEIRAVCSVSTSTSFMRPEKFLRLKEWKENGYFERES
                     SRYGKIKIPYEFVEDASQWNQEELIKNVHTPILIALGKEDDVVLPENTRLLYEAANNP
                     KELVEFDDVDHFYKKNPDLIRMVNKKIVEFFKKNL"
     gene            2315..2776
                     /locus_tag="VI432_01090"
     CDS             2315..2776
                     /locus_tag="VI432_01090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4496729.1"
                     /translation="MKDKANISQDIKIRIAALDDYRAIAKIQREDGFKHAYLLTEERF
                     KRLLGRGELFLIAHMGDNAVGIISIDIEFRAKIHFLSVMKSYQKRGIGSTLLKEAFKE
                     IKDKGVEVVYVYVETSSPAEQFLLQSGFDKVGVYQNRYGEGKNANILELRI"
     gene            2780..3379
                     /locus_tag="VI432_01095"
     CDS             2780..3379
                     /locus_tag="VI432_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4496730.1"
                     /translation="MNKIILIGGAPSLGKSHLAHELSKRLKIPWISTDTVRDIMEETA
                     DRKKYPELFYTEGQTPEKYLTRRTPREIVRDHLKRDRIVWLGVERMIKDAYIWKSFII
                     EGTSILPNLVNKKFGKDKHIKPIFIINNNEDHIRKVVYTRGLWTDADKYSDSVKEKEV
                     EWVKYFNNWIKNEAKKYKLPYIENTSKKDAIKKALKIIK"
BASE COUNT         1187 a          446 c          753 g         1007 t
ORIGIN      
        1 aacagatcaa ttattagacg aaaaaattaa aattgaaatc ttacttgatt caatcggaga
       61 cggcattata gctattgatc gttcgtggaa tattacattt tctaatgaaa ttgcgtcttc
      121 gatcagcgga tggcctaaaa aagaaatgat cggcaggccg tttcgcgatg ttataaaatt
      181 tatacgcgag tacgatcgga aagagaatat atcgttcatt gaaaatgcta tggttttagg
      241 caggatgcag caattacagg aaaagacttc attaattaaa aaaaacggag acgagatttt
      301 aattgatgat tctgcttctc caatttttaa tgatgatgga aaagtggttg gcgcgacgat
      361 aatttttaga gacgcttcta ctacacaaga ggcttctaat ctgcgcgctg gttttcaata
      421 cgcctctcat caattaaaca cgcctattag taagataatg tggagtttag agttggctat
      481 agaagagaag gattatgaca aaagaacaaa ggcgataaaa gaggcttata ggtctgcgca
      541 aagcgtatca aaaatgtcag aacagctttt tgcagtagct caaattgacc aagatgtcgt
      601 aatcccggaa tatgctgaat ttgaattaga cgtgcttatt aaagatataa gcaaaaaatc
      661 cgagaatagt tcttttaggt ctgacgtaaa gatatctttt aatatcgcaa atgatattaa
      721 aaaaattaat agcgataaga aaatgctgtc acgcatatta caagagcttc tcgataatgc
      781 ttttaaattc aatgcgcaag atggatctat tgatatttcg gttagattga tacatggaga
      841 tattgttttt gctattaaag atactggtat aggaatcgtt gagcgtgatc aagcgctggt
      901 ttttacaaaa ttcttccgtg ggatgaattt tgacacctct aaaatatcag gggccggatt
      961 gggtttattt atctcaaagg cttatacaga gattttggat ggtaagatct ggtttacttc
     1021 aaaacccggt gaaacaattt ttagttttaa gcttccttta atacatctgg agaccctgaa
     1081 atagattgta taaacttagg tgttataatg atttttatgg aaagaggcgg agttgaggct
     1141 gttgcatcgg ttttaatagt aaaagatgga aaaattcttt taataaaatc ccctaaatgg
     1201 gatgatagtt atttagttcc aggcggacac attgatagag gcgagactat tatagatgcc
     1261 gccactcgtg aaggagaaga ggaaacaggg ctgaagttaa aatcgcttta ctgcgtcaac
     1321 gtcggagagt caatttttga cccgtcattt tatagaaaag ctcatcttat ttattttcat
     1381 tttgtttgcc aggtagtagg cggagagatt agccccgatg ccagagaggt aagagaattt
     1441 ttttggtttg atccgaaaga agcgttaaat ttaaaattaa caaaaggagt caggaaaaca
     1501 atagaaaatt atataaatgg agtgcgtatt gatttagcaa gtgcaaaatt ttaggcatga
     1561 atttatctat aaatactata aataatttaa aattgtctgc cgttgttcat aaacccgaag
     1621 gcgatggtcc gttcccagcg gttatcttgc tacacggctt tacgggatat aaggatgaag
     1681 aacatattgt tattttagct aacgatttgg ctgaaaatgg tttggtggct gtaagatttg
     1741 atgcatctgg atttggtgag agtgaaggga acacagaacg cgattatcgc atgtctaacc
     1801 acttggaaaa cgcagagagt gcttataagt tccttataga acaagactac gtggatgaaa
     1861 aaagagttgg tgtttggggg cacagcatgg gagggggact cgctataatt tttgcttcta
     1921 agcatccaga gattagggca gtttgctcag tctccacatc aacaagtttt atgaggccag
     1981 aaaagttttt acgattgaag gaatggaaag aaaatggtta ttttgaaaga gagagctcta
     2041 gatacggcaa aattaaaatt ccgtatgaat ttgtagagga tgctagtcag tggaatcaag
     2101 aagagttaat aaaaaatgtg catacaccga tattgatagc gctggggaag gaagatgacg
     2161 ttgtattacc agaaaatacc agattgcttt atgaggcggc taataatccc aaagagttgg
     2221 ttgaattcga tgatgttgac catttctata aaaagaatcc agatttaatt aggatggtca
     2281 ataaaaagat tgttgaattt tttaagaaga atctatgaag gataaggcaa atatatctca
     2341 agatattaaa attaggatcg ctgctttaga tgattatcgg gcgattgcaa aaatacagag
     2401 ggaagatgga tttaagcatg cttatttact tacagaagaa agatttaaga ggcttctagg
     2461 caggggtgag ttatttttaa tcgcgcatat gggagataat gctgtgggta taatctctat
     2521 agatatcgag tttagagcta aaatccattt cttgtctgtt atgaagagtt atcagaaaag
     2581 aggtatagga agtactctat tgaaagaagc atttaaagaa ataaaagata agggcgtcga
     2641 ggtcgtatat gtatatgtag agactagttc gccagcggaa caatttttgt tgcagagtgg
     2701 atttgataaa gttggtgtgt atcaaaatag atatggtgaa ggaaaaaatg cgaacatttt
     2761 agaattgcgt atatgaaata tgaataaaat tattttaatc ggcggggctc catcgttggg
     2821 taaaagtcat ttggcgcatg aattatctaa acgtctcaaa attccctgga tctctacaga
     2881 tacggtaaga gatataatgg aggagacggc agatcgtaaa aaatatccag aattgtttta
     2941 tacagaagga caaactccag aaaaatatct tacaaggcgt acaccacgcg agatcgtaag
     3001 agatcattta aaaagagata gaattgtttg gttgggtgtt gaaagaatga taaaggacgc
     3061 atatatctgg aagtctttta ttattgaagg cacgtctata ttgccgaatt tagttaataa
     3121 gaagtttgga aaggataagc atattaaacc tattttcatt atcaataaca atgaagacca
     3181 tattagaaaa gttgtttata ctcgtgggtt atggactgat gcagataaat attctgattc
     3241 ggtaaaagag aaagaggtag aatgggttaa atattttaac aattggatta aaaacgaagc
     3301 aaagaaatac aaacttcctt atatagaaaa tacatcaaag aaagatgcaa ttaaaaaagc
     3361 attgaaaatt ataaaatgaa aataatttta gcg
//
LOCUS       DATDNZ010000027         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_853987, whole genome shotgun sequence.
ACCESSION   DATDNZ010000027 DATDNZ010000000
VERSION     DATDNZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853987"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VI432_01100"
     CDS             complement(<1..621)
                     /locus_tag="VI432_01100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4496731.1"
                     /translation="MRIGFFSETSLPLRYGIETSMASFGSKLEELGHKVYVYTSSAPG
                     YKEINRRIFRLRAFAGGKKTDMRFAVPIGENGRLKDFIKNKLDIAHLHTVLTLGLLGK
                     YIAWRQKIPTVYTHHNDYKGAIQAYLGKRSIIAYLVDKLAIWFANTSTAVIAPSEKMR
                     EILLSSGVKRPIHILPTGVDLKFFAVTTKTKNKALAIRKQYNISPRT"
     gene            complement(628..2145)
                     /locus_tag="VI432_01105"
     CDS             complement(628..2145)
                     /locus_tag="VI432_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY4496732.1"
                     /translation="MNKKINFPEPEEEIIDLVPSRNGLHKKLLFVLGGIFIGVIVLSV
                     FQVGIFRVMDTEPIDKNEVLKESQTGFVGSLIDKVLDKNDGKLVEIIDVKSGEVIKFD
                     EPSKDEFSVSADSSAGQITIKECDFDEAGVVVGGGIKINEVAWMGSSESSNHEWIELK
                     NVSASGIDISNWYLIDKDNQIKIRFPKNSLIKNNSFYLLERGEEASSSKADLIYSGSL
                     KNSDEGLRLFNTECALIDEVFAKEKWPAGDNLLKTTMERSIDGLWYSSQKVGGTPGLE
                     NSDSTPPYNQSNYYSQTSYYFEPTYYSESLYYSEATYYSETAYYSESTYYLESLYQAS
                     PPAQEPVYSQASYYSEASYQTGSVSNVLISEILPGLDGQSNYEFIELYNPSDEQIDLT
                     GWELRKKNSSGNESNLVDNGAFTGSIPAHGYFLVASPGYNGSPSPDIIYSVASGSFAY
                     DDNSIVLYNGDYRTANIIDEISWAEISAGQSYERDSWSSNSFHIQSNPNPQNSNN"
     gene            complement(2188..3102)
                     /locus_tag="VI432_01110"
     CDS             complement(2188..3102)
                     /locus_tag="VI432_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4496733.1"
                     /translation="MYDLIIAGGGPGGIAAGVYSARKKMKTLLITDSFGGQSFVSADI
                     QNWIGTKSVSGFEFAQMMEGHLRAQEDIEIIDDDRISLVEKKGDNFVITTNKGNKLET
                     KTFLVTTGSKRKKLNIPGEKEFDGRGVAYCATCDAPIFKNKVVAVVGGGNAGLEAVID
                     LLPYASKIYLMEYGDALKGDLLTQGKIKSNPKVEILLHAKTTKISGDNMVTGLEYEDL
                     KEKVTKKLELGGVFVEIGSIPNTDFLNGLVELNKFGEIIVDHKTQRSSLTGIWAAGDA
                     TDVLYKQNNISAGDAVKAVLNIHDYLNK"
BASE COUNT          970 a          762 c          553 g         1025 t
ORIGIN      
        1 cgttcttggt gaaatattgt attgcttacg aatcgcaagc gctttatttt ttgtttttgt
       61 tgttacagca aaaaatttca aatcaactcc agttggaaga atatggatcg ggcgtttgac
      121 gccggacgaa agaagaatct ctctcatttt ttccgagggg gcaattacgg cggttgaagt
      181 attggcaaac cagatcgcaa gcttatcaac caaataagct attatgctcc tcttaccaag
      241 atatgcctgg atcgctccct tataatcatt atgatgggta taaacggtgg gaattttttg
      301 ccgccacgct atgtatttac ccaatagccc gagggttaaa accgtatgaa gatgagctat
      361 atcaagttta ttttttataa aatccttcaa cctgccgttt tctccaatcg gaaccgcaaa
      421 acgcatatct gtctttttcc cgccggcgaa cgctctgagc ctaaaaatac ggcggttgat
      481 ttctttatag ccgggagcag aagacgtata aacataaacc ttgtgcccca gctcctccag
      541 cttggatcca aaagaagcca tagatgtctc aataccatat ctaagcggca gagatgtttc
      601 agagaaaaag ccaatgcgca taagtgatta attatttgaa ttttgaggat taggatttga
      661 ttgtatatga aacgagttgc tgctccaaga atctcgttca taactctgcc ctgcagagat
      721 ctctgcccaa gaaatctcat caattatatt cgcagtccta taatctccat tatataaaac
      781 aatactatta tcgtcataag caaaactccc agaggctacc gaataaatta tatccggtga
      841 aggcgaccca ttataacctg ggcttgcaac aagaaaatat ccatgcgctg gaatagatcc
      901 tgtgaaagcg ccgttatcaa ccagattgct ttcgtttccg gaagaatttt tcttgcgcaa
      961 ttcccatcca gtcaaatcta tctgctcgtc actaggatta tacaactcta taaattcata
     1021 atttgactgt ccgtctaaac cgggcaaaat ttcgctgatc agcacattag atacgctacc
     1081 agtctggtaa gatgcttcag aataataaga agcctggcta taaaccggtt cctgcgccgg
     1141 aggagaagct tgataaaggg actctagata ataagttgat tcggaatagt aagccgtctc
     1201 tgaataataa gtcgcctcgg aataataaag agactctgaa taataagttg gttcaaaata
     1261 ataacttgtt tgcgaataat aatttgattg attgtatggc ggagtcgaat cagaattttc
     1321 caaccctgga gtgcctccta ctttttgaga tgaataccac aatccatcta tagatctttc
     1381 cattgtggtt ttcaaaagat tatctccagc cggccatttc tctttagcaa aaacctcatc
     1441 aataagcgcg cattctgtat taaaaagtct taacccctcg tcgctgtttt ttagcgaccc
     1501 cgaataaata agatcggctt ttgaggaaga cgcctcctcg cctctttcca aaagataaaa
     1561 gctattattt ttgattaaac tatttttagg gaatctaatt tttatttgat tatctttatc
     1621 aattaaatac cagttagaaa tatctattcc cgaagccgag acattcttaa gttcaatcca
     1681 ttcgtggtta gaactctccg agctgcccat ccaagccacc tcatttattt ttatgccacc
     1741 gcctacaacg actccagcct cgtcaaaatc gcattcttta atggttatct gtccggcgct
     1801 agaatcagcc gagacagaaa actcgtcttt gctaggttcg tcaaatttaa taacctcacc
     1861 cgacttaaca tcaatgattt ccaccaattt cccatcattc ttatccaaaa ccttatcaat
     1921 aagcgaccca acaaaaccag tctggctttc ttttaaaact tcatttttat ctattggctc
     1981 cgtatccatt actctaaaaa ttccgacctg aaatacgctt aaaactataa ctccaataaa
     2041 aatgccgccg agaacaaata aaagcttctt atgtaatccg ttccgagacg gaaccaaatc
     2101 tataatctct tcttctggtt caggaaaatt aattttctta ttcatcgggc tgtttgaagt
     2161 tttgtttatc cttctatccc ggaattatta tttattcaga tagtcgtgga tatttaaaac
     2221 cgctttaact gcatctccgg ccgaaatatt attctgttta taaagcacgt ctgtcgcatc
     2281 tcccgcggcc cagattccag tcaagcttga tctctgtgtt ttgtggtcaa ctattatctc
     2341 gccaaactta ttcagctcaa ccaaaccatt caaaaaatcg gtgttgggaa tagagccaat
     2401 ctcaacaaat actccgccta attccagctt cttggttact ttctctttta aatcttcgta
     2461 ttccaaccct gtaaccatat tatcccccga tatttttgtc gtctttgcgt gcaacaaaat
     2521 ctcaactttc ggatttgatt ttatttttcc ttgcgttaac aagtctcctt tcagcgcatc
     2581 tccatattcc attagataaa ttttagaggc gtagggcaaa aggtctataa ctgcttccaa
     2641 acccgcgttg cctccgccta caaccgccac aactttattc ttaaaaatag gcgcatcgca
     2701 agtagcgcaa tacgctactc ccctaccatc aaattctttc tctcccggta tattcaattt
     2761 ttttctttta ctgccggttg taactaaaaa ggtctttgtc tcaagcttat ttcctttgtt
     2821 cgtagttatg acaaaattat ctcctttttt ctcaaccaaa gaaattctgt catcatcaat
     2881 aatttcaatg tcttcctgcg ctctcaagtg accctccatc atctgcgcaa actcaaaacc
     2941 tgaaacggat tttgttccta tccagttttg aatatcggcc gaaacaaaag attggccgcc
     3001 aaaactatcg gtaatcaaaa gtgtcttcat cttcttccta gcagaataaa ctccggccgc
     3061 aattccacct ggtccgccac cggcgataat taaatcgtac ataaaaaata gtatataata
     3121 taaagtcttt tgtataaagt atctgaacta agatactatt aaaatcgtga acagtttaaa
     3181 gactataaca aaaaatacgg tttggctctt cagcggacaa atagtagggc gggctctacg
     3241 tgcggcgatc gtcatctacg ccgccagagc tttgggcgca tacaattggg gagccttttc
     3301 ttatgctctc
//
LOCUS       DATDNZ010000028         3527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_592724, whole genome shotgun sequence.
ACCESSION   DATDNZ010000028 DATDNZ010000000
VERSION     DATDNZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592724"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /gene="thrS"
                     /locus_tag="VI432_01115"
     CDS             <1..1275
                     /gene="thrS"
                     /locus_tag="VI432_01115"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY4496734.1"
                     /translation="VAFETKEELDEYLAMMEEAKKRDHRILGEQLKLFTFNDLVGKGL
                     TLWLPNGTIIKNEIEKFAIEMENKYGYQRVSTAHIAKKELFETSGHLPYYADSMYPPM
                     KMDDGIYYLKAMNCPLHHVIYKAEPKSYRDLPVRLAEYGTVYRNELSGTLAGLLRVRS
                     LQMNDAHIYCRKDQIETEIQKVLEMTIEYFQIFGLKDYWFRLSRWSPKNKEKYINEPK
                     NWEYTEEVLRKVLKASGAKFVEVEDEAAFYGPKIDVQFKSVIGREESMSTIQLDFTAK
                     TRFNLTYTDDSGKENNEVFIIHRAPLSTHERFMAFIIEHFAGVFPLWLSPVQVKIIPV
                     SDKFNKYAEEVRGKLSDTDIRVEVDDRNETLGKRIREAETQKTPYIAIVGEKEMSANT
                     LSIRKRGGKDLGAIRIDEFIERLQKEIEKKSI"
     gene            1226..2194
                     /gene="miaA"
                     /locus_tag="VI432_01120"
     CDS             1226..2194
                     /gene="miaA"
                     /locus_tag="VI432_01120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY4496735.1"
                     /translation="MNSLKDFRKKLKKNLSKPKIVAILGPTSSGKSDLSVNLAKKFDG
                     EIISADSRQVYKGLDIGSGKVPLDRKLPSGYSLVANYYYKGVPHHLLDIASPKKTFTV
                     AEYKKLGKQTIEDTLKRGNLPIITGGTGLYIDALIYDMDFPKVPPDYKLREELEEKNT
                     EELFKILKGLDKKRAESVDPNNRRRLVRAIEIIKSTGEPISVIKRESPYDVLKIGIKI
                     SREELKNRIQKRLDKRLKLGMIDEVEDLHNKGLSWRRLDDLGLEYRFISRHLRGIINY
                     EEMRESILRESYKYAKRQMTWWRKDKGINWIENPEEVDRLVKDFIK"
     gene            complement(2432..3142)
                     /locus_tag="VI432_01125"
     CDS             complement(2432..3142)
                     /locus_tag="VI432_01125"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY4496736.1"
                     /translation="MKVTPIKTRIFKEREDILTFIKRYFKRIPEKSILVVTSKIVSYS
                     EDRLKSIENKKTKENLIREESDVMMRTKYIWLTIRDNMVMPSAGVDESNANGKIILLP
                     KDSYKSAERIRRELRKYYKLKKFGVLITDSRSMPLRKGIVGVAMGYAGFKGIRDYQGT
                     PDIFGRILNLSITDVADSLATSAVLCMGEGRERQPLALIEDAPVEFVEKTNRKELEIN
                     LKEDLYQPLFEKIKKLRW"
     gene            complement(3148..>3527)
                     /locus_tag="VI432_01130"
     CDS             complement(3148..>3527)
                     /locus_tag="VI432_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEY4496737.1"
                     /translation="AGLIKASKTSIKDIKITPENLAELVVMIRLGKLSSRGAKDMLVK
                     MFETGGDPSSLLESMGVAQISDEGELKKIVMDVIEGNPNVVADYKNGKAPALEALIGR
                     AMGKLKGKGNPAILRKLFLENIA"
BASE COUNT         1180 a          665 c          679 g         1003 t
ORIGIN      
        1 gttgcctttg aaacaaaaga ggagcttgat gaatatctcg cgatgatgga agaagctaaa
       61 aagagagatc atcgaatatt gggtgaacaa cttaaactct ttacctttaa cgatctcgta
      121 ggcaagggat taacgctatg gctccccaac ggaacgatta ttaaaaatga gatagagaaa
      181 ttcgcgatag aaatggaaaa caaatatgga tatcaaagag tctcgactgc tcatatcgct
      241 aaaaaggaat tattcgaaac atccggtcat ctcccctact atgcagattc aatgtaccca
      301 ccgatgaaga tggacgatgg tatttattat ctaaaagcca tgaactgccc gcttcatcac
      361 gtaatatata aagcagaacc caaaagttac agagatttac cagtaagact cgctgaatat
      421 ggaacggttt acaggaatga attatccggc acgctagcag gattattacg cgtacgatca
      481 ctgcagatga acgatgcgca tatctactgc cgcaaggatc aaattgaaac agaaatacaa
      541 aaagttttgg aaatgactat tgaatatttc caaatttttg gactcaaaga ttattggttc
      601 agattatcaa gatggagtcc caaaaataaa gaaaaatata taaacgagcc taagaattgg
      661 gaatatacgg aagaagtttt aagaaaggtc ttaaaagcaa gtggagctaa attcgttgaa
      721 gttgaagatg aagccgcgtt ttatggacca aaaatagatg tccagtttaa atccgttatc
      781 ggtcgggaag aaagcatgtc tacgatccag ttagatttca cagctaagac taggtttaac
      841 ctaacctata ctgatgattc tggtaaagaa aataatgagg tatttataat acatcgcgcg
      901 cctctttcca cacacgaaag attcatggct tttataatag aacactttgc gggcgtattt
      961 cctctctggc tctctcctgt acaagtaaag ataattccgg tttcggataa gtttaataaa
     1021 tacgcggagg aagtacgcgg gaaattatct gatacggata tacgcgtaga agttgatgat
     1081 agaaatgaaa cgctcggtaa aagaatccgc gaagcagaaa ctcaaaaaac tccttatata
     1141 gcaattgttg gagaaaagga aatgagtgcg aacacattga gcatccgcaa gcgtggaggt
     1201 aaagatctcg gcgcgattag aattgatgaa ttcattgaaa gacttcagaa agaaattgaa
     1261 aaaaaatcta tctaaaccaa agattgttgc gatccttgga ccgacatcat ccggaaaatc
     1321 agatctatca gtcaatctgg ctaaaaaatt tgatggcgag ataatctccg ccgactcgcg
     1381 acaggtttat aagggtttag acattggttc gggcaaagta ccattagata gaaagctgcc
     1441 tagtggctat tcgctagtcg ctaattacta ttacaagggc gtcccccatc atttattaga
     1501 tatagcctcg cccaaaaaaa catttactgt cgccgaatat aagaaattgg ggaagcaaac
     1561 aatagaggac acattgaaac gcggcaattt accgataatt accggtggaa caggattata
     1621 tattgacgcg ttaatttatg acatggattt tccaaaagtt ccgccggatt ataaactacg
     1681 cgaggaatta gaagaaaaaa acacagaaga attatttaaa atattaaagg gattggataa
     1741 aaaacgggca gagagtgttg atcctaataa ccgcagacgg ttagtcagag cgattgagat
     1801 aataaaatca accggagagc caatctctgt aataaaacgc gaatcgccct atgatgtttt
     1861 aaaaattggg ataaagatat ctagagaaga attaaagaac aggatccaaa aacgtctgga
     1921 taaaagactg aagcttggga tgatagatga ggtggaagat cttcataata aaggacttag
     1981 ttggagaagg ttggatgatt tggggttaga atatagattt atttcaagac atcttcgtgg
     2041 aattataaat tatgaagaaa tgagagaatc gatattacgt gaaagttata aatacgccaa
     2101 acgccaaatg acttggtgga gaaaagataa aggcatcaat tggatagaaa atccggaaga
     2161 agtagataga ttggttaaag attttattaa ataaattctt catccccctt ttatccccat
     2221 ggggataaaa gggggatgaa gaaaatacat atacagaaat gaaaatccga gcagataaat
     2281 ttccgcccgg aagatatttc atgtcccgca acagctacag gagccgcaat ggccaatccc
     2341 agctctacat atattgcaag ccattttgat agttcacctc cttgttaagg tagtactcaa
     2401 agactaatat aatctattcc tctagtctat cctaccaacg caactttttg attttctcaa
     2461 ataatggctg gtagagatct tctttcaggt ttatttccaa ttcttttcta ttggtctttt
     2521 ctacaaactc cacaggagca tcctctatta aggccagggg ttgccgctct ctaccctcgc
     2581 ccatgcataa gactgcagat gtcgcgagac tatctgcaac atcagttatc gataaattta
     2641 aaattcttcc aaaaatatca ggtgttcctt gataatctct aatgccctta aagccagcgt
     2701 agcccattgc aacgcccacg attcctttgc gtagaggcat tgagcggcta tcggttatca
     2761 aaactccgaa tttctttaat ttataatatt ttctcaattc acgtctaatg cgctctgcgc
     2821 tcttgtatga atctttggga agaaggatta ttttgccgtt tgcatttgat tcatcaactc
     2881 cggcagaagg catcaccata ttatcacgga tagtaagcca tatatatttt gtcctcatca
     2941 ttacatcact ctcctcgcga ataagatttt ctttagtctt tttattctca atactcttca
     3001 atctatcttc tgaataagaa acaattttag aagtaacaac taaaatagac ttttcaggaa
     3061 tacgcttaaa atatctctta ataaaagtta ggatgtcctc cctctctttg aaaattcggg
     3121 ttttaatcgg cgttaccttc attaaaatta ggctatattc tctaagaata attttcttaa
     3181 aatcgctggg ttacccttcc cttttagttt gcccatggct cttccgatta aagcctctaa
     3241 cgcaggggct ttgccattct tataatctgc aactacgttc ggattacctt ctataacatc
     3301 cataacaatt ttcttgagct cgccttcatc actaatttga gctactccca ttgattctaa
     3361 aagcgaacta ggatctccac ctgtctcaaa cattttaact aacatgtcct ttgctcccct
     3421 acttgaaagt ttgccgagtc ggatcattac aaccagctcc gccagatttt cgggagttat
     3481 tttgatatct tttatactgg ttttagatgc ttttataaga ccagcta
//
LOCUS       DATDNZ010000029        10041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_85608, whole genome shotgun sequence.
ACCESSION   DATDNZ010000029 DATDNZ010000000
VERSION     DATDNZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85608"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VI432_01135"
     CDS             complement(<1..1580)
                     /locus_tag="VI432_01135"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY4496738.1"
                     /translation="MKKIVLVILLILAMILTITPTIFGAELVGVAIAQEDQLSLEDEI
                     NGIEISDLGVDSPGILPTNPFYFLKEWGRGIKSVFTFNSVAKAEYELRILNQKAAELK
                     NVRESNPDNVRGIDKAIENYNKNAIRLKSRIESLSESSDNSQVDRLLDGLVSKTIKHQ
                     QLFEELKSKYESAKDKVEGVQDTIDETIMSLPQKFEDVDKFAERVERAIQSQRDTPLK
                     ELKIAQVLNRLESVSTSETETRAALTLVKDDLFIKFEGRINAGDLPLAGIADLIYRLP
                     GGEVEALKIVDDIREKTSDSELKNKLNALRPEVLTSIVETSAIDNEEAERMIAEAERV
                     LNNLDELIVSDKYDVTSQIKNLAEKARFNLEDAKTILSNGEFGRAFGQANASLVVARN
                     AISQLYREPQDELQNAITGLKNRFDNLRTLAREEGLTDEKNPKLFVLFSEAEKLILNI
                     KTIEDVRKAKVVLSEIEIVLRAGVVTGYVGDSYTDEACTKEYAPVCGADGRTYGNICV
                     ARSSGAEIIKRGACEDGLT"
     gene            complement(1653..2087)
                     /locus_tag="VI432_01140"
     CDS             complement(1653..2087)
                     /locus_tag="VI432_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY4496739.1"
                     /translation="MNKNRFVLGAVLFLGVLYYVYALNERTNLHILEINGEKIGVYVA
                     LTDEERARGLSGLEALGVDEGMLFIFEEIGIYHFWMKDMLFPIDIIWIDDEFKVSEII
                     HNVGPETFPQLFRPQRPIRYVLEVNAGFAEDKQLKPGQEVNF"
     gene            2156..2599
                     /locus_tag="VI432_01145"
     CDS             2156..2599
                     /locus_tag="VI432_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4496740.1"
                     /translation="MNMHKILFCKCTEGWAKYAPLVLRLSAGTIFAMHGYQKFSGGID
                     GVAGFFTQLGIFAPTFFAYVVTYVELLGGIALILGIFTHWAAKLLAIDMLVATWIVHI
                     PNGFFITNGGISYTLILFAVAISLMLSGPGIWSLDEQLTKKESKI"
     gene            complement(2635..3219)
                     /locus_tag="VI432_01150"
     CDS             complement(2635..3219)
                     /locus_tag="VI432_01150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4496741.1"
                     /translation="MKTETGRPIFLLGHKVNLRPFRREDIPALTRWINDPEIRDFILV
                     FLPQSENQEEEWFKALGKDDKSVVLAIETKDGILIGSMGLHDIRWKDRIATTGAIIGE
                     KDYWGKGYGTDAKMTLLNYAFNGLNLYKICSSVISFNKRSLKYNLRCGYQIEGIRKRN
                     IFKRGRYWDVVMLAVFKKDWLPLWKMYLKTGKSK"
     gene            complement(3478..3813)
                     /locus_tag="VI432_01155"
     CDS             complement(3478..3813)
                     /locus_tag="VI432_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY4496742.1"
                     /translation="MAKIVTVEELKKKIDNKEDFYLIDALGENSFKARHIPGAKNIPN
                     DIDFMERFEREIGAPKNAEIITYCSSDHCMASVQAAKYLEEAGYTNVSHFKGGIAGWQ
                     EAGYEFSGI"
     gene            complement(3840..4118)
                     /locus_tag="VI432_01160"
     CDS             complement(3840..4118)
                     /locus_tag="VI432_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEY4496743.1"
                     /translation="MEQNKHQVQINASPEELKGRYSNVMQIQHTKEEFVLDFFNILQA
                     SGALVSRVILSPGHLKRMLDALQENLKRYEENFGKIKPAEEPSKKIGF"
     gene            complement(4133..5284)
                     /locus_tag="VI432_01165"
     CDS             complement(4133..5284)
                     /locus_tag="VI432_01165"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY4496744.1"
                     /translation="MMRFMVLIGNAHDRVYSAHFLSYHLIMSRKTLIFLTAVLVLGAF
                     VFVLQKYISKNLRPSGDTNINTSSWVLYENKDFGFRIKYPSDWMIYEDPNNVIQPAFN
                     IYPKSIAPGKLPLIHHSVEPNVSIFPAGVPTEGVIGKTTNSSIDFINLYPKNAFDYTL
                     ENGDVWATFVYHWLDIQSRQSGLQKWTDAGFLWARAEIDDYVEICLRDGSEMPVESCD
                     FFGIDVADEIVYRGRVNKETRAIQEEILKSFEFTVQTPGALVRVDYPKHGDVITNPLI
                     VTGEARGNWYFEATFPVALVNWDGLIIAEWYAEAQLDPNDPESTWMTTDFVPFRAELK
                     FENPSFQEGSVENDFSRNGYLIISKSNASGLSEHDSSIEIPVRFEKQSK"
     gene            complement(5335..5643)
                     /locus_tag="VI432_01170"
     CDS             complement(5335..5643)
                     /locus_tag="VI432_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496745.1"
                     /translation="MKIRMMMKCGCEFTQFRITFCGEAMRLYDAKESLAEATQVLLAS
                     FVENELNEHLHADCHLGNAALCIELTAIWNKFVEIAKRRGSEKVGNSWVIRRHAKILK
                     "
     gene            5764..6651
                     /locus_tag="VI432_01175"
     CDS             5764..6651
                     /locus_tag="VI432_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEY4496746.1"
                     /translation="MKSNTIRWIILFILILIITASTYYAFLFKSLGEIKEDVSDSEQA
                     DIETDELNKAVQNIFYTNTDLLPDLIPLPPDDVQIINENDNVFLLFTTMFYNRGKGPL
                     ELRVDEEAARIKTDVDQDVIQRIYKEDGTFRERLSGKFLWHQTHLHYHFSDFVIYDLE
                     PVGAPDDPSLSGVRSKATFCIRDVSSVDLDLKDRLPEGKYLICGKKLQGISIGWADTY
                     FSNYPDQRLNLTGLASGTYRLNFIVNPEDSFEEETLNNNISSALLYIDMQNETVEILE
                     TNPINYPKVEHIYIEQSFE"
     gene            6679..7827
                     /locus_tag="VI432_01180"
     CDS             6679..7827
                     /locus_tag="VI432_01180"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4496747.1"
                     /translation="MAKKLSGIIIFIILTSLFFFIWSKDTKAPSDTMPEISGSENAIK
                     ENAPFNAIIENLTIPWDIAFLPDNEGLIITERGGRILYQNSDKELMEIANINIKSGGE
                     GGLLGLTLHPDFSENRFVYLYLTAPGNANQTENRVERYRFENNNTLTDKKVVISGIPG
                     AVYHDGGRMEFGPDEKLYITTGDATNANLAQDLNSLAGKILRLNDDGGIPTDNPFKTA
                     IYSYGHRNPQGLTWDMAGNLWSTEHGPSSPIGSDCCRDELNLIKPGANYGWPTIKGNE
                     KAGGMESPIIHSGNSTTWAPASAAYVVSRNSIFFGGLRGETLYEAVLDGVKVKELKEH
                     FKNEFGRIRTVRLGPDGMLHITTSNRDGRGSVHSGDDKIIRINPNSLN"
     gene            complement(7829..8113)
                     /locus_tag="VI432_01185"
     CDS             complement(7829..8113)
                     /locus_tag="VI432_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496748.1"
                     /translation="MKNDKYRKARAGHSKILQLTCAKCEKDLFRYQKDGLGILKRLYL
                     DRIIDNRNYSKNLVCPKCRSVLGIKIIYKKENRPAYRLFAGTVFKTIVKK"
     gene            complement(8116..9885)
                     /locus_tag="VI432_01190"
     CDS             complement(8116..9885)
                     /locus_tag="VI432_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY4496749.1"
                     /translation="MLEQALISIIIIATLVLIVWGKWRYDIVALVALFATLLFGLIPI
                     ENIFNGFIHPVVILMVVMVILSKGLIRAGVVDVIARYLKIRGKHPAVQLGVLIVFTAF
                     LSSFINSMGALAFVIPIAIRMAKQSGSAISMFLMPVAFASHLGGGVTLISSISNIVVS
                     GFRAEQASPFGFFDFATISLPIVAVAIIFIVVGGWRLIPKREDVFARDAALERYVTEV
                     KISKGSPAIEKTLADFQKFLKEHITILSLVRFEHKFPDPSPDTVLREGDILIIETDPE
                     TLEAIIATAKLELIGKEPLSGEGGSKEVEGLSLMEAVVADESSLIGESAASIKLRTYY
                     NVNMLALHHGRGDIGLRMSDITLRGGDVLILQGYEDDLARFIKAFSLLPLKEREFQLE
                     GSKELALALGIFVSAIAVSITGLFPAYIIFVFAGLLMLLFGLIPVKEMYQSVDVSVVV
                     LLGVMLQLGLVFQETGAAKTFADLLLIFSDQITPTIALGIIFAISILLADILSNATVA
                     VLLSPVALAVAIELGVSIDPFLMAVAIGSASSFLTPFGHQSNVFVMGIGGYKFKDYWR
                     LGLPLEIITFGVGMWAILTFWPF"
BASE COUNT         3168 a         2269 c         1588 g         3016 t
ORIGIN      
        1 gtcaaaccat cttcacacgc gccccgtttt ataatctctg caccgctcga tctagcaaca
       61 cagatattcc cataagttct gccgtctgct ccacaaaccg gggcatattc cttagtacaa
      121 gcctcatcag tatagctgtc accaacatag ccggtaacca ctccagctct taaaactatt
      181 tctatttctg atagaacaac ttttgccttt cttacgtctt caattgtttt aatatttaat
      241 atcaatttct ctgcttcgct gaataggaca aacaatttag gatttttttc gtctgttaat
      301 ccttcttccc tagctaatgt tcttaagtta tcaaacctgt tttttaggcc tgttatagcg
      361 ttttgaagct cgtcttgagg ctctctatat aattgagaaa ttgcgtttct cgcaacgact
      421 aaggatgcat tcgcctggcc aaaagcgcga ccgaattcgc cattacttaa aatagtctta
      481 gcgtcttcga gattaaacct agccttctct gcgagatttt tgatctgact cgttacatca
      541 tatttatcag aaacgataag ctcgtccaga ttgtttaaaa cgcgttccgc ctcagctatc
      601 atcctctctg cctcttcatt gtctattgcg cttgtctcga ctatagaagt taaaacttct
      661 ggtctcaatg cgtttaattt gttttttaat tctgaatctg atgttttttc cctaatatca
      721 tccactattt tcaaagcctc aacttctcca ccaggcaatc tataaatgag atcagcgata
      781 cccgccaatg gcaaatctcc ggcatttatc cttccttcaa atttaataaa cagatcatct
      841 ttaacaagcg ttaaggccgc tctcgtttct gtctccgagg ttgatactga ttctaatcta
      901 ttcaaaacct gcgctatctt taactcttta agcggggtgt ctctttgact ttgaatggcc
      961 ctctctactc tctcggcaaa tttatcaaca tcttcaaatt tttggggcaa agacatgatc
     1021 gtctcatcaa ttgtatcctg cactccctca actttatctt ttgcagattc gtatttagat
     1081 tttaattctt caaataactg ttgatgtttt atggttttcg aaacgagacc atccaacaat
     1141 ctgtcaactt gagagttgtc agaagactcg ctgagagatt ctattctcga ttttaaacgg
     1201 atagcgtttt tattataatt ttctatagct ttatctatcc ctcttacgtt atccggatta
     1261 gactctctca cattttttag ctcagccgct ttttgattaa gaatcctcaa ttcgtattcc
     1321 gcctttgcaa cggaattaaa agtaaaaaca ctctttatgc cccttcccca ctctttcaaa
     1381 aaataaaacg gattggtcgg caaaatacct ggagaatcaa ctcctaaatc gcttatttct
     1441 atgccgttta tctcatcttc gagcgataac tgatcttctt gagctatagc aacacccact
     1501 aattcagcgc caaaaatggt cggtgttatg gtcaatatca ttgctaatat taacaaaatt
     1561 actaatacta tttttttcat aatactgata atcctatcat aatttataaa cggaacaaat
     1621 tagatttgct tacacgatta tactaaaaac ccttagaaat taacttcctg tccgggcttt
     1681 aattgtttat cttcagcaaa tccggcatta acttccagca catatctaat gggccgttga
     1741 ggcctaaaaa gctgagggaa tgtttccgga ccaacattat gaataatttc agaaacctta
     1801 aactcatcgt cgatccagat aatatcgatc gggaaaagca tatccttcat ccaaaaatga
     1861 tatatgccga tctcttcaaa gataaataac atcccctcat caactcccaa cgcctctagt
     1921 ccagataaac ctcttgccct ttcttcatcg gttaaagcaa cataaactcc gattttctct
     1981 ccattaattt ccaatatatg caggtttgtc cgctcattta acgcgtaaac ataatacaaa
     2041 acacccaaaa ataataccgc gccaagaaca aacctattct tattcatgaa ttaattataa
     2101 caattaattt gctataatta attaaacggt cgattataaa ttattcttat aaaaaatgaa
     2161 tatgcataaa atattattct gcaaatgcac ggagggatgg gctaaatacg ctccactcgt
     2221 cctacgtctt tcggccggaa cgatttttgc catgcacgga tatcaaaaat tctcaggagg
     2281 catagatgga gtcgctggat tttttactca attaggaatc ttcgctccaa ctttcttcgc
     2341 ttatgttgta acctatgtgg aacttttggg aggaattgcc ttaattctgg gaatcttcac
     2401 tcattgggct gcgaaactat tggcaattga tatgctcgtc gccacctgga tagtccatat
     2461 cccaaacggc tttttcatca caaacggtgg gatatcttac accctgatac tttttgccgt
     2521 agctatttca ctaatgcttt ctgggccggg catatggtct cttgacgaac aattaactaa
     2581 gaaagaaagt aaaatttaaa cgataaaggc tctaaaaata gagcctttaa aaatttactt
     2641 agatttaccc gttttcaaat acatcttcca tagtggcagc cagtctttct taaagacagc
     2701 caacataacc acatcccaat aacgacctct cttgaaaata tttctctttc ttatgccctc
     2761 aatttgatat ccacatcgca aattgtattt cagtgaacgc ttattgaaag agataactga
     2821 cgagcaaatc ttgtaaagat ttaaaccgtt aaacgcataa ttcagaagag tcattttcgc
     2881 atcagtccca taacccttgc cccagtaatc cttttctccg ataatggccc ctgttgtggc
     2941 gatcctgtcc ttccaacgta tgtcatgcaa tcccattgat ccgataagaa ttccatcttt
     3001 tgtctctatg gcaagaacaa cactcttgtc atcttttccc aaggccttga accattcttc
     3061 ttcctgattt tcactctgag gcaaaaacac aagaataaaa tctcttattt cagggtcatt
     3121 aatccagcga gttaaagctg gtatgtcttc tctcctaaag ggtctcaagt tcaccttatg
     3181 gccgagaaga aaaatcggcc tgccagtctc ggttttcatc tttacacctc ttttaaagga
     3241 acaaaattgc cccggttctg ccggggcaat ttatttatcc ttttgtaata atccttctat
     3301 ttatggttta agcgtaaaag atatatagat tgacccagca aaaataaccc gtgccacacg
     3361 gcggttagtg aaatattcat ttgtgggaat ctaaaattag tcatcatttt aaattggcgc
     3421 gcatttttag cgcttcataa tatataacgt aactttataa gctttggtta tttatatcta
     3481 tattcctgag aattcataac ctgcctcttg ccatccggca ataccaccct taaagtgact
     3541 tacattcgta tatccagcct cttccaaata tttagccgcc tgcacgcttg ccatacagtg
     3601 atcagaagaa caataagtga tgatctctgc atttttcggc gcgcctattt ccctctcaaa
     3661 tctctccata aaatctatat catttggtat attctttgcc cccggtatat gccttgcctt
     3721 aaaactattc tctcctaagg catctatcag ataaaaatcc tctttattat ctattttctt
     3781 ttttaattcc tctactgtaa caatttttgc catagttaaa ataattatta atttataatt
     3841 tagaatccta ttttctttga tggctcttca gccggcttta tcttgccaaa attctcttca
     3901 tatcttttaa gattttcttg taatgcgtct agcatccttt ttaggtggcc ggggcttaat
     3961 attactcgcg aaaccaaagc tcctgaagct tgaagaatat taaaaaaatc taaaacaaat
     4021 tcttctttag tgtgctgaat ctgcatcaca ttagagtatc tcccttttaa ttcttcgggt
     4081 gatgcattta tctgaacctg atgtttattt tgttccatat tggtattttt aattatttcg
     4141 actgtttttc aaagcgtact ggtatttcta ttgaactgtc atgctcagac aagccagacg
     4201 cattactttt actgataata agatatccgt tcctgctaaa atcgttttcg actgagcctt
     4261 cttggaaaga aggattctca aatttaagct cagccctaaa aggcacgaaa tcagttgtca
     4321 tccaagttga ttcagggtca ttaggatcca actgcgcttc cgcataccac tcggcgatta
     4381 taagtccatc ccaatttacg agcgcgaccg ggaaagttgc ctcaaaatac caattgcccc
     4441 tcgcctcacc tgttacgata agtggattcg ttattacatc tccatgcttc ggatagtcaa
     4501 ctctaacaag cgcgccagga gtttgaaccg taaattcaaa cgatttcaaa atttcctcct
     4561 gaatcgccct cgtttcctta ttaaccctgc ctcgatagac aatctcatcg gccacatcta
     4621 taccaaaaaa atcgcaagat tcaactggca tttcactccc atcacgaaga caaatttcta
     4681 cataatcatc aatttccgcc cttgcccaca aaaatccagc gtcagtccat ttctgaagtc
     4741 cgctctgccg gctttggata tccaaccaat gataaacgaa tgtcgcccag acatctccgt
     4801 tttcaagagt ataatcaaaa gcatttttag gataaagatt aataaaatct atcgatgagt
     4861 ttgttgtctt gccaatgaca ccctctgtag gaactcctgc tggaaaaata gaaacattag
     4921 gttccaccga gtgatgaatc aagggcaatt ttcccggagc aatagacttt ggataaatgt
     4981 taaaagccgg ctggatgaca ttattcggat cttcataaat catccagtca gacggatatt
     5041 taatcctgaa accaaaatct ttattctcat ataagaccca actgcttgta ttgatatttg
     5101 tatcgccact tggtcttaaa tttttagata tgtatttttg aagcacgaaa acgaatgcac
     5161 ccaatactaa aactgccgtc aaaaatatca aggtttttct actcataatt aaatgatagc
     5221 ttaaaaaatg cgccgaataa accctgtcat gcgcatttcc aattaaaacc ataaaacgca
     5281 tcatcggatg atttttttaa aaaacaaaag ggagcgttca taaacgctcc tttcttactt
     5341 caaaatctta gcgtgacggc ggataaccca tgaatttcca accttttcgc tccctcttcg
     5401 tttagctatt tctacaaatt tattccatat ggccgttaac tcaatgcaca gggccgcatt
     5461 gccaagatga caatcagcgt gcaagtgctc attgagttca ttttcaacaa aacttgctaa
     5521 aagcacttgt gtcgcttccg ccaagctctc cttagcatca taaagcctca tagcttcacc
     5581 gcaaaacgtg atcctaaatt gggtaaattc acatccgcat ttcatcatca tccgaatctt
     5641 cacctcatta ttaatgtcct tttaaagtta atatattata tacacagata tgtcaacttt
     5701 aattaatacg ccatgaatac attttatatt ttatagagta aaatatataa aacgactaga
     5761 gatatgaaat caaacacaat tcggtggata attttattta tccttatcct tataatcacg
     5821 gcgtccacat actatgcatt tctttttaaa agcttaggcg aaataaaaga agatgtgtcc
     5881 gattccgaac aggccgacat tgaaacagat gagcttaaca aggcggttca aaatattttc
     5941 tatacaaaca cagatttgct gccagatctt atccctctac cgccagatga tgttcaaatt
     6001 attaatgaaa atgacaatgt ttttttgtta ttcacgacta tgttttataa ccgcggcaag
     6061 gggccgctag agctgcgcgt tgatgaagaa gcagcaagaa tcaaaacaga cgttgatcaa
     6121 gatgtaatcc aacgcatata taaagaagac ggtactttcc gcgaaagact ctccggtaaa
     6181 ttcctgtggc atcaaaccca cctccattac cacttctctg atttcgttat ttacgattta
     6241 gagccggtcg gagcgcccga tgatccaagt ttatctggag ttcgcagtaa agcgacgttt
     6301 tgcataagag atgtaagttc tgttgattta gacctgaaag atagattacc cgaaggcaaa
     6361 tatcttattt gcggaaaaaa acttcaagga atttcaatcg gctgggcaga cacgtatttt
     6421 tcaaattacc cagaccaacg cctaaatcta acaggccttg cctccggcac atatcggctt
     6481 aacttcatag tgaatcctga agacagcttc gaagaagaaa ctctaaacaa taatatatca
     6541 agcgctcttt tatatatcga tatgcaaaac gaaacagttg aaatcttaga aacaaatccc
     6601 atcaattatc caaaagtaga acatatttat atagaacaat cttttgaata gagattataa
     6661 aaatattaga ataagcaaat ggcaaaaaaa ctatcaggaa taataatttt tataattcta
     6721 acaagcttat tcttctttat ttggtcaaaa gacaccaaag ctccatcaga tactatgcca
     6781 gaaatatcgg gatcggaaaa cgccattaaa gaaaacgcgc cattcaacgc aattattgaa
     6841 aatttaacaa tcccatggga tattgccttt ctgcccgata atgaagggct cattataacc
     6901 gaacgcggag gtagaatcct ctatcaaaat tctgacaaag aattaatgga aatagctaat
     6961 ataaatatca aatcaggagg agaaggaggg cttctaggat taactctgca tccagacttc
     7021 tctgaaaata gatttgttta tctttaccta accgctccag gaaacgcaaa tcaaacggaa
     7081 aaccgcgttg aaagatatcg ctttgaaaac aacaacaccc ttacagataa aaaagttgta
     7141 atatcgggca tccctggagc cgtatatcat gacggcggaa gaatggaatt cggaccagat
     7201 gaaaaacttt atataacaac aggtgacgca accaacgcaa atctggcgca agatcttaat
     7261 tctttagccg gaaaaatttt acgcttgaat gacgacggcg ggatcccaac cgacaaccca
     7321 tttaaaaccg ctatatattc ctatggacac agaaatcccc aagggctcac ttgggatatg
     7381 gctggaaatt tatggtctac agaacatggt ccatcttcac cgataggatc cgattgttgc
     7441 agagacgaac ttaatttaat caaacccgga gcgaattatg gctggccgac aataaaaggt
     7501 aacgaaaaag caggaggaat ggaatcacct attatacact ctggtaattc tactacttgg
     7561 gcgccagcta gcgccgctta tgtcgtctca cgcaacagta tcttcttcgg cggtctgcgc
     7621 ggagaaactc tttatgaagc tgttttagac ggtgtaaaag ttaaagaatt aaaagagcat
     7681 ttcaaaaatg aattcgggcg aatcagaacc gtgcgtttag gaccggatgg tatgcttcat
     7741 atcacaactt caaaccgcga tggccgtggc agcgtccatt ccggagacga taaaattatt
     7801 cgtattaatc caaattctct caattaattt attttttaac tattgtctta aacacggttc
     7861 cggcaaacaa tctataagca ggtctgtttt cttttttata aataattttt attcctaaaa
     7921 cacttctaca ttttggacaa acaagattct tggaatagtt gcgattatca atgattctat
     7981 ccagatacaa tctctttaat attcccagtc catctttctg gtatctaaat aaatccttct
     8041 cgcacttagc acaagttaac tgcaaaattt tagaatgtcc ggctctagct tttctatatt
     8101 tatcattctt cataattaaa acggccagaa agttaatata gcccacatac cgaccccaaa
     8161 agttattatc tcaagcggca atcccaatct ccaataatct ttaaatttat atcctcctat
     8221 tcccatcaca aagacattag actgatgacc aaatggagtt aaaaaagacg aagccgagcc
     8281 tatcgcaacc gccatcaaaa aaggatctat agaaacgccc aattcaattg caaccgcaag
     8341 cgcgacaggc gacagaagca cggcaacagt cgcattactt aatatatcag caagtaaaat
     8401 gcttattgca aaaataattc caagcgcaat agtcggagta atttgatccg aaaagattaa
     8461 taataaatca gcaaaagtct ttgcggcgcc ggtttcttga aaaacaagac ccaactgcaa
     8521 cataacaccc aaaagcacaa caacagacac atcaacggac tgatacatct cttttactgg
     8581 aatcaaacca aaaagaagca ttaaaagccc agcgaaaaca aagatgatat atgccgggaa
     8641 aagccctgtt atagaaactg caatcgctga aacaaatatt cctaaagcaa gggcaagctc
     8701 cttactccct tcaagctgaa actctctttc tttgagaggt aaaagtgaga atgcttttat
     8761 aaaacgcgct aaatcatctt cataaccctg aagaatcaac acgtctcctc ccctcaaagt
     8821 gatatcgctc attctaagtc caatatctcc cctcccatga tgaagagcga gcatattcac
     8881 attgtaataa gttcttaact taatactcgc agctgactcg cctattaaag aagattcatc
     8941 tgctacaact gcttccataa gacttagacc ctcaacctcc ttactgcctc cctctcctga
     9001 aagcggctcc ttaccgatta actccaattt agccgtagca attatagctt ctaaagtttc
     9061 cgggtctgtc tcgataatta aaatatctcc ttctctcaaa accgtatccg gagaaggatc
     9121 cggaaattta tgctcgaaac ggaccagaga aagtatagta atgtgttctt tcaaaaattt
     9181 ttgaaaatcc gccaaagttt tttctatcgc aggcgagcct ttggaaatct ttacttcagt
     9241 aacatatctt tcaagcgccg catcacgcgc aaaaacatct tctcttttag gtatcaacct
     9301 ccatccgcca acaacaataa aaataatggc aacggccaca atcggcagag aaatggtcgc
     9361 aaagtcaaaa aatccgaatg gcgaggcctg ctccgctcta aatccggaaa cgacaatatt
     9421 agaaatagaa cttataagcg tcactcctcc cccgagatga gacgcaaaag caaccggcat
     9481 caaaaacata gaaatcgccg atccgctttg ctttgccatt cttatcgcaa taggaataac
     9541 aaaagcaagc gcgcccatgc tatttataaa tgaggataaa aaagcggtaa atacaatcaa
     9601 aactccaagc tgcacagcag ggtgcttccc tcttatcttc aaatatctcg caattacatc
     9661 gacaactccg gccctaataa gccctttgct caaaataacc atgacgacca taagaattac
     9721 aaccggatga ataaatccat taaaaatatt ttcaatgggg ataagtccaa acaatagagt
     9781 agcgaaaagc gcgacaaggg caacaatatc atacctccac ttgccccaga ctatcagaac
     9841 tagtgttgct ataataatta tcgaaattaa agcctgctca agcatataca cgattttaac
     9901 acagctccgt cagattctta acttatcaaa attacggata catttttttt atttcacgat
     9961 ctaatgcata tatcaacgaa atttaatgta cttcaaatga attaaagtca cgtatatccg
    10021 attcttgaac ctcaatttta t
//
LOCUS       DATDNZ010000030        10038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3240572, whole genome shotgun sequence.
ACCESSION   DATDNZ010000030 DATDNZ010000000
VERSION     DATDNZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240572"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="VI432_01195"
     CDS             <1..1940
                     /locus_tag="VI432_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496750.1"
                     /translation="SSYYEQSSYYSESTYYSQSSYYAQSSYYNQSSYYNQSSYYAQSS
                     YYSQSTYYSESTYYSQSSYTPPTSPERYWIGGSGYWWDTNHWSLTSGGAGGATVPTST
                     EDAYFDVNSFTAGGQYVDMCVNSGPAVSVYMKNMDWTGVTNSPEIRCVSGIKTKMYIS
                     SNLTLSSNMTQTRGTDYDVDWRFNSPNTITINQNGIKIGPTFFEGTGIYNLANNFTVY
                     ANSITQINSGTFNASGFNLDSGSFRSTGSLARTINMGSGTWTTSTGSGNAWEISGSNI
                     TLNADQSTLNIVNRAASVANFIGGGKNYYNVILREPGYIQNEQTLKVTDSNTYQNLTL
                     TANTSNETSLISFASGTTQTVNGTFTANGTAGHPVTLRSATAGSQHTINAASSSVSYM
                     DAKDSNATGSASPFQCLTGCTDSGNNTGWTFNTSEDFSTDPNITDPPNGSATNNVLIW
                     DSGNGRLQTRAQRGNSDANWRHIDVGELNTNFTSTFKFTGVSGSAGSGGFKHLFIGFT
                     QSSDPDILINSAIADPSDGLGIQIGTDGGNLGSTDNCWTFKGVRVDNVSINSTSTNLI
                     CIPGHQRTDIYRVTIEYNVVSGSWSIQIFNETASSEFLAASGTTAGGRSYRYFGASTG
                     GGASNSGTWTGYIDDLTVDAN"
     gene            2023..3843
                     /gene="typA"
                     /locus_tag="VI432_01200"
     CDS             2023..3843
                     /gene="typA"
                     /locus_tag="VI432_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY4496751.1"
                     /translation="MDIRNIAIIAHVDHGKTTLVDALLKQSENFKLKSDAVQELIMDS
                     NELERERGITIFSKNASIKYKGVKINIVDTPGHADFGGEVERIMKMVDGVLLLVDAKD
                     GPKPQTKFVLRKAIEAGNKAIVVINKIDKPDARPEWALNQTFDLFIDLGANDEQTNFP
                     VIYASGAQGKAGTTPDITLMKDVRPLFDKVIEYVPAPKIDESKPLQMLTINLAYDNYK
                     GKIAIGRLNSGVIKKGMQVAHITRNGDIKKAQVSSLMSFDGLSRVEVEQVGAGDILGV
                     AGIPDISIGETIADINNPIALPTIKIDEPTIKMTFAVNTSPFMGREGEYTTSRNLRER
                     LIREVETDVALSVAPTDSPDKWIVSGRGELHLSIFIEKMRREGYELEVSKPQVILKEK
                     DDKRTEPIELVSIEVPEKFSGAIIEMMGKRLGAMRDMRLDRETMYMDFMVPTRGLIGV
                     RNEFMTSTKGLGVMNSLFMGYEDYKGNIDVNPHGSLIASESGASNSYGLINAQSRGQL
                     FIGPGIEVYEGMVVGQNAKTEDILVNICKAKELTNFRTKNFGVQEKLDVPRDMSLENA
                     LDYIGENELLEITPKSLRIRKSILKESDRKRLKKQSDSNK"
     gene            3935..4714
                     /locus_tag="VI432_01205"
     CDS             3935..4714
                     /locus_tag="VI432_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY4496752.1"
                     /translation="MNNKLKVDLSWPLIAILAGATFAFITLATSYSKSIEPSSFRNFS
                     VSAEGEAIGIPDVAKFNFTVITEGGKDIGALQTDNSTKINKAISALKTSGVKDEDIKT
                     ASYNLNPRYQYFNCSNGFNQSTKPCPPPEIVGYTITQSVSVKIRDFSKIGLILSEIVK
                     VGVNDVSNLQFEIDDPIALQNEARANAIEKAKEKALSIAKSAGFSIGRLISIQEGFNY
                     DVYARSYGQLLDTAEGTPIPPQIEPGSQEIKISVTLKYEIR"
     gene            4757..5410
                     /gene="truB"
                     /locus_tag="VI432_01210"
     CDS             4757..5410
                     /gene="truB"
                     /locus_tag="VI432_01210"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY4496753.1"
                     /translation="MIDGIFAINKPKGPTSNRVLQEIKKFTGAEKIGHAGTLDPLASG
                     VLIIGIGQATKKLSDIVKDEKEYIAEIKLGEKSVTDDEEGIKQQLTIDRKPTIDEIEN
                     SIQGFVGKIEQIPPIYSAIKIQGKEAYKLARKGEFPEMEKRSVEIKEIKLLNYEWPIL
                     EIKAVTGPGTYIRSLARDIGESLGVGGYLANLKRTRVGKFTIDKALNPEDLISYAKK"
     gene            5397..5699
                     /locus_tag="VI432_01215"
     CDS             5397..5699
                     /locus_tag="VI432_01215"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY4496754.1"
                     /translation="MQKNSNFKDRVVKIVKSIPRGKVLTYKQVAMMAGSPNAMRAVGN
                     VMKNNFDPTVPCHRVIASNGIGGYNGGVMKKIRKLKEEGVNIDKIYEKQEPNKKSW"
     gene            5665..6297
                     /gene="nth"
                     /locus_tag="VI432_01220"
     CDS             5665..6297
                     /gene="nth"
                     /locus_tag="VI432_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY4496755.1"
                     /translation="MKNKNLIKKAGEIIRELKKLFPKTRIALNYSNNLELLVAVILSA
                     QCTDKKVNEVTEKLFKKYKTLDDYVKAKPLGFEQDIKSTGFYRNKTKNILAAAKMIKK
                     DFGGRVPKTMAEILKLPGVARKTANIVLHNAYGVIEGIAVDTHVIRLSTRLGLSKHKD
                     PVRIERDLMELIPKKDWAEFNCRLVDYGRAYCKAKKHDHDACPLKEFDVK"
     gene            complement(6391..6963)
                     /gene="cas2"
                     /locus_tag="VI432_01225"
     CDS             complement(6391..6963)
                     /gene="cas2"
                     /locus_tag="VI432_01225"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY4496756.1"
                     /translation="MDDRKRKKRLRIGGVSQKILLLLGGGLSLGLTHRPDIFFRVAKD
                     IGREWSEINHKSLRDAIRNLYASKMVDYKEEKGGKISIVLTGDGKKRALRYSVKNIKI
                     KKPKKWDGMWRIVIFDIPEDNKQGRDALAKKLKELGFKPIQKSVFVYPYECRDEIDFI
                     TEVFNLRPYVRLLLVKDIDIEPELLHHFKL"
     gene            complement(7115..8215)
                     /locus_tag="VI432_01230"
     CDS             complement(7115..8215)
                     /locus_tag="VI432_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4496757.1"
                     /translation="MPRVSGPANVVHNLYTGLKDSGHEVYVFAPKYTGAIKSEGDIIR
                     IPSLRMPSNIFYPIPLPSFSDAEEIIERLDLDIFHSHHPTMIGKLALFGAKLSNKPLV
                     FTYHSEWGSYLRAMFPKSPIESIDKLFEGLLESYIRESDAVIVPNKVLYKKIDHKNIY
                     LLPSAIRPFMTKEPRKILEEYKLSNDDKILLCVSRLSPEKGIDFLIEMIAPILDGRDD
                     LKLLLVGDGISKSKIKYLVKKFDLEDRVIFAGEVGYDDLPSFFKLAHIFVYPSRFDTQ
                     GLSVVEAMSFGLPIVSFNTPFFKEVVRDSGSGITVDNETDFKEAVEKLINDNKARVRM
                     GESAKLKSVDFDHKKSISTIIDIYKNLLSGQA"
     gene            complement(8338..>10038)
                     /locus_tag="VI432_01235"
     CDS             complement(8338..>10038)
                     /locus_tag="VI432_01235"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY4496758.1"
                     /translation="TGAVYDIEEVDVTASQIMDEIINYAWLNGEPGIVFIDKVNKTNP
                     LPDLGDIQTSNPCGEQFLHPYDNCNLGSINLASFVKDHKVDWKRLEFATRTATRMLDN
                     VIDIFDFPVKQVTDLAHKNRRIGLGIMGFADMLYQIGVKYDSKEGFDLAKKVMAFIED
                     ASHKMSRELAKEKGVFENWKLSVYGKKKEKMRNAALTTVAPTGSISMMLDTSSGIEPN
                     FALAFIKQDKDGLQYNYLNKYFEAELHRRKFDEAKIERIKEEIIKTGSIQHVTDLPQD
                     LRDTFVVSMDISGEDHIRMQASFQEEVDNSISKTINLPNKATKEDVRRSYVLAWKLGC
                     KSCTVYRDGSRNIQILNLGTSENVVSTIEDPSIKKLKEAPLNLDIDKDRISPKERPEV
                     VSGRTYKAKTGYGNLYVTINNDERGAPFEVFAVIGKSGGFFQEQSEAICRLISLALRS
                     GVKVEKVIDDLRGIRGPMPVMTSKGTVLSLPDAIGQILEEHVRMNGGKVEEAVASISV
                     DQSKGNVLSESSGQMQLSSAKKTIADFGYMPGCPDCGNALVMSEGCISCKSCGFSRCL
                     "
BASE COUNT         3356 a         2077 c         1883 g         2722 t
ORIGIN      
        1 aatcatcata ctatgagcaa tcatcttact actccgaatc aacttattac tctcaatcga
       61 gttactacgc ccagtcgtca tattacaatc aatcttctta ctacaatcaa tcgtcatact
      121 atgcgcagtc aagttattac agccaatcaa catattactc agagtcaaca tactatagcc
      181 aatctagtta tactccaccg acatcgcctg aaagatattg gataggcgga tcgggttact
      241 ggtgggatac aaatcattgg tcactaacta gcggcggcgc gggaggagca acggtcccga
      301 catcaactga agatgcatat tttgacgtta attctttcac cgctggagga caatatgtag
      361 atatgtgcgt taactccggt ccagcggtca gcgtgtacat gaaaaatatg gactggactg
      421 gggtcacaaa cagtccggaa ataagatgtg tatctggaat aaaaacaaaa atgtatattt
      481 caagtaatct aacgcttagc agcaatatga cacaaaccag gggaacagat tacgacgtag
      541 attggcggtt taattcaccc aatacaatta ctataaatca aaacggaatc aaaataggcc
      601 caaccttttt cgaaggaaca ggcatataca atctagcaaa caacttcacg gtctatgcaa
      661 atagcattac tcaaatcaat agcggaacat ttaacgctag tggctttaac ttagattccg
      721 gctcattccg ttcaactggg tctctagctc ggactatcaa catgggtagt ggcacgtgga
      781 caactagcac ggggtcggga aacgcttggg agataagtgg ctctaacata actttaaatg
      841 ccgatcaatc cacattaaat attgttaacc gcgcagcaag cgttgctaat ttcataggcg
      901 gaggtaaaaa ctattacaat gtaatattac gagaaccagg ttatattcaa aatgagcaaa
      961 cccttaaagt aactgactct aatacatacc aaaacctaac gctaacggca aatactagta
     1021 atgagacttc tttaatatct tttgcctcag gtaccacgca aaccgtaaat ggcacattta
     1081 ctgcaaacgg aaccgcaggc cacccagtca ccttaagatc tgccacagcc ggatcacaac
     1141 acacaataaa cgccgcttct tctagtgttt cttatatgga tgcgaaagat agcaacgcta
     1201 ctggctctgc ttctccattc caatgtctta cagggtgtac cgattctgga aataataccg
     1261 gatggacatt taataccagc gaggatttct caacagaccc caacatcaca gatccgccga
     1321 acggctctgc aacaaataat gtacttatat gggacagcgg gaatggacgc cttcagacca
     1381 gagctcaaag aggaaatagc gatgcgaatt ggagacatat agacgtcggt gaactaaata
     1441 caaacttcac atcaactttc aaatttactg gagtaagtgg cagcgccgga agcggcggtt
     1501 ttaaacatct ttttatagga ttcacgcaaa gcagtgatcc agatatccta atcaacagcg
     1561 ctatcgccga tccgagcgat ggattgggaa tccagattgg tacagacggt gggaacctag
     1621 gatcaacaga caattgctgg acattcaaag gggttagagt agacaatgtc agcatcaatt
     1681 caacctctac taatttaata tgcatacccg gtcatcagag aacagatata tatagagtaa
     1741 ctattgaata taatgttgtt tctggttctt ggtctataca gatattcaac gaaacagcaa
     1801 gttcagaatt ccttgcagca tctggcacta cagctggagg cagatcatat cgctatttcg
     1861 gcgcatcaac cggaggtgga gcaagcaact cgggaacctg gacaggatat atagacgatc
     1921 ttactgtaga tgcaaattaa tattaatact tgaaaataac aatatatcgt ggtatcttta
     1981 ttcagtaaac ctgctcgaaa atcgggcaga ttttttatca atatggatat ccgaaacatt
     2041 gcgataatcg cgcacgttga ccacggtaaa acaacgcttg ttgacgcgct tctaaagcaa
     2101 tctgaaaact ttaaattaaa atccgacgct gtccaagaat taatcatgga ttcaaacgag
     2161 cttgaacgag agcgcggtat tactattttc tccaaaaacg cttcgattaa atataaaggt
     2221 gtaaaaataa atattgtaga cacgcccggt cacgctgatt tcggcggaga ggtggaacga
     2281 ataatgaaga tggttgatgg cgtgctcttg ctagttgacg ccaaagacgg tcccaaaccg
     2341 cagactaaat ttgtattaag aaaagcaatt gaggctggta ataaagcgat tgttgtcata
     2401 aataaaatcg ataaaccgga cgcacggccg gagtgggcgc taaaccagac ctttgatctt
     2461 tttattgatt taggggctaa cgatgaacaa accaattttc cagtaattta cgcctcaggc
     2521 gcacaaggaa aagccggaac tactccggac attactttaa tgaaagatgt gcgaccatta
     2581 ttcgataaag ttatcgaata tgttccagcg cccaaaatag acgagtccaa accgcttcag
     2641 atgctaacca tcaatcttgc ttatgacaac tataaaggca aaattgctat cggcagatta
     2701 aattcaggcg taataaaaaa gggtatgcaa gttgcgcaca tcacaagaaa cggcgacata
     2761 aaaaaagcgc aggttagttc gctaatgagt tttgatggat taagcagggt cgaagtcgaa
     2821 caagtaggcg ccggcgacat attaggagtt gccggaatcc cggatatctc aatcggagaa
     2881 actattgccg atataaacaa tcccatagct cttcccacaa ttaaaataga tgagccaacc
     2941 attaaaatga cttttgcagt aaacacgtct cccttcatgg ggcgcgaggg agaatacaca
     3001 acatctagaa acttgagaga gcgcctaata cgcgaagtcg aaaccgacgt agccctatct
     3061 gtcgccccaa ccgattcacc cgacaaatgg attgtctctg gcagaggaga gcttcattta
     3121 tcaatcttta tagaaaaaat gaggcgcgaa ggttatgaat tagaagtatc taaaccgcaa
     3181 gtaatcctta aagagaaaga cgacaagaga accgaaccca tagagcttgt atctattgag
     3241 gtgccagaaa aattctccgg cgcaatcata gaaatgatgg gaaaacgtct gggggccatg
     3301 agagatatga gactcgacag agaaacgatg tatatggatt ttatggtgcc cacaaggggc
     3361 ctaatcggcg tcagaaacga atttatgacc tctactaagg gtttaggagt aatgaattca
     3421 ctattcatgg gatatgaaga ctataaagga aacatcgatg ttaatcctca tggttctctt
     3481 atcgcctccg aatccggagc gtcaaacagc tatggactaa ttaatgcgca gagtcgcggc
     3541 caattattta taggtccggg aattgaagta tatgaaggta tggtcgtggg tcaaaacgca
     3601 aaaaccgagg atatactagt aaatatctgt aaagcaaaag agctgaccaa cttcagaact
     3661 aaaaactttg gagtgcaaga aaaattggat gtgccaagag atatgtctct agaaaatgcg
     3721 ctagattata taggagaaaa tgaattgctt gaaatcactc ctaaaagctt aagaataaga
     3781 aaatctatcc tcaaagagtc tgatagaaaa aggcttaaaa agcagtccga ctccaataaa
     3841 taagttcttg aaacttttaa tgggcttatg cgtcttctat ataaggctgt ctatttgata
     3901 taataatgat agtcgtataa ataaaataaa tataatgaat aataaattaa aagtagattt
     3961 gagctggccc ttgatagcga tactcgcggg agcgacattc gctttcataa ctttagcaac
     4021 aagctattcc aaatcaattg agccctcatc gtttagaaat tttagtgtct ccgctgaagg
     4081 agaagcgata ggcataccgg atgtggcaaa attcaacttc acagtgataa ccgagggagg
     4141 gaaagatata ggcgctcttc agacagataa ttcaacaaaa atcaataaag cgatttccgc
     4201 tctaaaaacc tctggcgtga aagatgaaga tatcaaaacg gcgtcttata atttgaatcc
     4261 aagataccaa tacttcaact gtagcaatgg attcaatcaa tcgactaaac cctgtccccc
     4321 gccagaaata gtaggataca cgataactca atcagtatct gtgaagataa gagatttctc
     4381 taaaatagga ctgatcctct ctgaaattgt taaagttgga gtgaatgatg tatctaatct
     4441 ccaattcgaa atagatgatc ctatcgctct tcaaaacgaa gcaagggcaa acgcgataga
     4501 gaaagctaaa gaaaaagcat taagcatagc aaagtccgcc ggattctcaa ttggtagact
     4561 tataagcatc caagaaggat ttaattacga tgtctatgca agatcttatg gccagctctt
     4621 agatacggca gagggcacgc caattccacc gcagatagag cccggatctc aagaaatcaa
     4681 aatatccgtt actcttaaat acgaaataag atagggggta ctcagcttaa tgtatttgat
     4741 ataaaattcc atcaacatga ttgatggaat ttttgctatt aataaaccca aagggcctac
     4801 atctaatcga gttcttcaag aaatcaaaaa attcactgga gccgaaaaaa taggccacgc
     4861 cgggactctt gatcctctcg cgagtggcgt cttaattata ggaatcgggc aagcaactaa
     4921 aaaattgtct gatatagtaa aagacgaaaa ggaatacata gctgaaataa agctcggaga
     4981 aaaaagcgtc acagacgacg aagaaggaat caaacaacaa ctgacaattg accgtaaacc
     5041 gacaattgac gagatagaaa actcaattca aggattcgtt ggtaaaatag agcaaattcc
     5101 cccaatatac agcgctatca aaatccaagg caaagaagca tataaactcg ctcgtaaagg
     5161 ggagtttccc gaaatggaaa agaggtcggt ggagataaaa gaaattaaat tgctaaacta
     5221 tgaatggccg atcttggaaa taaaagcggt aactggccct ggaacctata ttagatcact
     5281 cgcgagagac ataggcgagt ctctcggagt aggaggttat ctcgcgaatc tcaaaagaac
     5341 aagggttgga aaatttacta tagataaagc gctcaaccca gaagatttaa tcagctatgc
     5401 aaaaaaatag taattttaaa gacagggttg taaaaattgt aaaatctatc cccagaggaa
     5461 aagttttgac atataaacag gtagcgatga tggctggaag cccaaacgcg atgagagcag
     5521 tcggcaacgt catgaaaaat aatttcgatc cgactgtccc ctgtcataga gttatagctt
     5581 caaatggaat cggcggatat aatggcgggg ttatgaaaaa aataagaaaa ttaaaagagg
     5641 aaggcgtaaa catagataaa atatatgaaa aacaagaacc taataaaaaa agctggtgaa
     5701 ataataaggg aacttaaaaa attatttcct aaaacaagga tcgccttaaa ttactcgaat
     5761 aatctagaac ttttagtggc cgtaatttta tctgcgcaat gcactgataa aaaagtaaac
     5821 gaggttaccg agaaattatt caaaaagtat aaaacgcttg atgattatgt taaagcaaaa
     5881 ccgttaggat tcgagcaaga tataaaatca accggctttt atcgcaataa aacaaaaaat
     5941 attttagccg ctgcaaaaat gattaaaaag gactttggcg gaagagtacc aaaaacaatg
     6001 gcggagattt taaaactacc cggagtcgcc aggaaaacag ccaatatcgt gctccacaac
     6061 gcatatggag taattgaggg tattgcggtc gatacccatg ttatacgcct ctctactcgt
     6121 ctggggctca gcaaacataa agatccggtg agaattgaac gcgacttgat ggagctgatt
     6181 cctaaaaagg actgggctga attcaattgc cgcttagtgg attacggccg cgcatactgc
     6241 aaggctaaaa aacacgatca cgacgcctgc ccacttaagg aatttgatgt gaaataaaaa
     6301 ccgcctttgt tttagaaata taaatagctg tcgttataac agcgatcgct gttataacga
     6361 caccacaacg gttaacgata aatctaaaac ctagagttta aaatggtgca ataattccgg
     6421 ctcgatatct atatccttaa ccaaaagtag tcggacgtat ggccgcagat tgaaaacttc
     6481 tgttataaag tcaatttcat ccctgcattc atatggatag acaaaaacac ttttctgaat
     6541 gggtttaaaa ccaagttctt ttaatttctt cgcaagggca tctcttccct gcttattatc
     6601 ctcagggata tcaaatatta cgatcctcca catcccatcc catttcttag gtttttttat
     6661 tttgatattt ttaacactgt aacgaagtgc gcgctttttg ccgtcacctg taagcactat
     6721 gctaatcttt ccaccttttt cttctttgta atcaaccatt ttcgaggcgt atagatttct
     6781 gatagcatct cttaagcttt tatggtttat ttcactccat tcccttccga tatctttagc
     6841 aactctgaaa aatatatctg gcctatgggt taaacctaat gataatccgc cgccgagcag
     6901 gagaagtatt ttttgcgaaa caccgccaat tcttaatctt ttcttacgct ttctatcatc
     6961 cataaattca tttaaaccgc atataagcca gatggatatt ataacacaat ctctaccgtt
     7021 ataacagcga tcgctgttat aacggtaggt cgctaattca agtaatagct cgatagttgt
     7081 aaaatatcta ttgcaagata aagcccaaca accatcaagc ttgtccactc aaaagattct
     7141 tataaatatc tattatagtg gatatactct tcttatgatc aaaatctaca gactttaatt
     7201 tggcagactc gcccattctg accctcgctt tattatcatt aataagcttt tctaccgcct
     7261 ctttaaagtc cgtttcatta tcaactgtaa ttcctgagcc gctatctcga accacctcct
     7321 taaaaaaggg cgtattaaaa gagactatag gaagaccgaa agacatcgcc tcaacaactg
     7381 agaggccttg agtatcaaat ctggaaggat aaacaaatat atgcgccaac ttaaaaaaac
     7441 ttggcagatc atcatatccg acttctccgg caaaaatcac cctatcttct aagtcaaact
     7501 tcttaaccaa atacttaatc ttagacttag atataccatc tccgaccaaa agcagtttta
     7561 aatcatctct tccgtctaaa ataggcgcga tcatctcaat taaaaaatct attcctttct
     7621 ccggagaaag ccttgaaacg cataaaagta ttttatcgtc attgcttaat ttatattctt
     7681 ctaagatctt tcttggttct tttgtcataa acggcctgat tgcagaaggt aaaagataga
     7741 tgtttttgtg atctattttt ttatataaaa ctttattagg cacaatgaca gcgtctgact
     7801 ctctgatgta gctttctaag agaccttcaa acaacttatc gattgattcg ataggactct
     7861 tgggaaacat tgctcttaag tacgaccccc attcagaatg atatgtgaaa accaacggtt
     7921 tatttgatag tttagctccg aaaagagcaa gttttccgat catagttgga tgatgggaat
     7981 ggaaaatatc caaatccaac ctctctataa tctcctccgc gtcagaaaaa gatggtagag
     8041 gaattggata aaaaatatta gaaggcattc taagcgaagg aatacgtatt atgtctcctt
     8101 cggattttat cgctcctgta tatttaggcg cgaaaacata tacttcatgt cccgaatctt
     8161 ttagaccagt atatagattg tgaacaacat tagccggacc actgactctc ggcaagtaat
     8221 tattagtaaa tagaccaatg cgcattagaa atagctagat atccgttagc ttttagaata
     8281 taagatgtgc ctaagaacta caaatgaaga tagatctaaa aagttatagg ttaatttcta
     8341 aagacagcga ctgaatccgc aactcttgca acttatacat ccttcagaca taacaagcgc
     8401 gttcccacaa tccggacatc ctggcatata tccaaaatcg gcgatagtct tctttgctga
     8461 acttaattgc atttgtccgg acgactctga taaaacattg cctttggatt gatcaacact
     8521 tatagatgcg accgcctcct caacttttcc gccattcatc ctaacatgct cttctaaaat
     8581 ctggccgatc gcatcaggaa gagaaagcac tgttccctta cttgtcataa cgggcattgg
     8641 acctcttatc cccctcaaat catcaataac tttttcaact tttacaccag acctgagagc
     8701 taaagaaatc aatctgcaaa tcgcttccga ctgttcctgg aaaaaacctc cgctctttcc
     8761 aataacagca aacacttcaa aaggcgctcc gcgctcatca ttattaattg tcacataaag
     8821 attgccgtat ccagtttttg ctttataggt tcttccgctt acaacctctg gtctttcctt
     8881 tggagagatt ctatctttat ctatatcaag attaagaggg gcttctttaa gctttttgat
     8941 agatggatct tctattgtag aaacaacatt ctcggatgtt cctaagttta gaatctgaat
     9001 attcctactt ccgtccctat aaacagtgca gctcttgcat cctagcttcc aagccaaaac
     9061 ataacttctt ctaacatctt ctttcgtggc tttattcgga agattaatcg tcttcgaaat
     9121 agaattatca acttcttcct gaaacgaagc ctgcattctg atatgatctt ctccagaaat
     9181 atccattgaa actacgaatg tatcgcgcag atcctgaggc agatcagtta cgtgttggat
     9241 gctgccagtt tttataattt cctccttgat cctctcaatt tttgcttcat caaatttcct
     9301 cctgtgcaat tcagcttcaa aatatttatt taaataatta tattgaagcc catctttatc
     9361 ctgtttaatg aatgcgagag cgaaattagg ctcaatgcct gaagaggtat caagcatcat
     9421 agagatcgag ccggttggag caactgttgt aagcgccgca ttcctcattt tctccttctt
     9481 ctttccataa acactcaatt tccaattttc aaaaacaccc ttttctttag ctaattctct
     9541 cgacatcttg tgagaggcgt cctcaataaa agccattacc ttctttgcca aatcaaatcc
     9601 ctcttttgaa tcatatttaa caccaatttg atagagcata tctgcaaagc ccatgatccc
     9661 aaggcctatg cgcctatttt tatgagcaag atctgtaact tgcttgactg gaaaatcaaa
     9721 aatatcaata acattatcaa gcatcctggt tgctgttctt gtcgcaaact ccaatctttt
     9781 ccagtcaacc ttatgatctt tgacaaatga agctagatta atagacccca aattacagtt
     9841 gtcatacgga tgtaaaaact gttcgccaca aggattagac gtttggatat ctcccaaatc
     9901 tggtaatgga tttgtcttat taaccttatc aataaaaact attcctggct caccgtttaa
     9961 ccatgcgtaa tttataatct catccataat ctggctcgca gtcacatcaa cctcctcaat
    10021 atcataaact gcgccagt
//
LOCUS       DATDNZ010000031         6247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1918700, whole genome shotgun sequence.
ACCESSION   DATDNZ010000031 DATDNZ010000000
VERSION     DATDNZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918700"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1129)
                     /gene="dnaX"
                     /locus_tag="VI432_01240"
     CDS             complement(8..1129)
                     /gene="dnaX"
                     /locus_tag="VI432_01240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4496759.1"
                     /translation="MKAMSLATYRKYRPKILDDLIGQDMIVEVLKNAARKDKISHAYL
                     FYGPRGTGKTTAARILAKIVNCETRQKDQKFKTEGEPCNECSHCLETEAGRALDIVEI
                     DAASNRGIDEMKNLKEGARLSPSSYQYKVFIIDEAHQLTKEAGNALLKMLEEPPSHAI
                     FILATTEYEKLPPTIVSRTQRLHFRRLPVIKIVEKLNKVVAAENIKIDQPAIELIASA
                     SEGSLRDAESLLDQVISLGEEATLESVERVLGKVGFIRTSTLAHYILEGDLPAVMEYL
                     SEIHESGFNIVQLNKDLIHYFRRALSMKFYPAIEEIFRRELTEDEIKELKKHSGMIIP
                     EKHITLIKSLIRAYSEMRYSPFPIVPLEVAIIENLKEAH"
     gene            1151..2503
                     /locus_tag="VI432_01245"
     CDS             1151..2503
                     /locus_tag="VI432_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEY4496760.1"
                     /translation="MKYDIMRRFFYILLPFALLSGVYLLLAQERSFDVNYDGINEFDP
                     VFSDGVVLAVPEASINEVKDSQIKSVKASADIPYQKQLDPTPETIKAIYVTSWSAGNK
                     KAIDRLIDLIRETELNAMVIDIKDYSGHVTYDIEVPDVLKYKAKEVRISKINSLIKKL
                     HDEGIYVIARLTVFQDPILSSIRPDLAVQNISTGEPWLDRKGLGWIDPSAKEAWDYNI
                     AIAKDALLRGFDEINFDYIRFPSDGDLSLMKFPFYNIETVYKKDAIREFFAYLRGELE
                     GYKISADLFGLSSINKDDLGIGQVIEYAYEFFDYVSPMVYPSHYAKGFLALDEPAKYP
                     YEVIKYSMHTAMVRLIAYSQDVIMQALDELQSASGGESPKVKLPNSKLRPWIQDFDLG
                     GVPYGVEEVRAQINALYDSAKSFECQGENQTLCLNPLNGWMLWDPANIYTKEALLSTE
                     "
     gene            2647..3348
                     /locus_tag="VI432_01250"
     CDS             2647..3348
                     /locus_tag="VI432_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY4496761.1"
                     /translation="MNVYEFEVGEFKGPIDKLLELIESKNMEITRLNLASVTSDFLDY
                     IRNLREADSKTISDFISVASQLILIKSHALLPNLELNPDEEKSIEDLERRLATYREFR
                     LVEKKIAKLWEDFPVFSREFVLGEGGGFYLSDEVTPRMLFEKIGELVKDFQSIMPKYE
                     SHKIKLINFEEKVKELIGRIEKTISSSFNEITKGRERSEVIVMFLALLHLLKDSLIKI
                     NQENAFSDIEITKIN"
     gene            3359..3931
                     /gene="scpB"
                     /locus_tag="VI432_01255"
     CDS             3359..3931
                     /gene="scpB"
                     /locus_tag="VI432_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY4496762.1"
                     /translation="MDQNINKLTSLIESLLFVHGEPMKIGKIAEIIGVDQGDVEQCLG
                     ILKDRLDREDSGISLMTFDGNASLITKPEFGSVIQKMVKEEIDSPLTPASLETLSIIA
                     YVGPISRAGIEYIRGVNSSFILRSLLIRGLIVKETGLDKGRVLYKVTFDFLKHVGVSS
                     AEQLPEFENYKGILNNLLNPTESAGDDNKK"
     gene            3928..4818
                     /locus_tag="VI432_01260"
     CDS             3928..4818
                     /locus_tag="VI432_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4496763.1"
                     /translation="MTMKPIYQAMLLTVIILFFALINGGEEVGSALDNSHTSFGAASL
                     EGYRSLDVSPDIKDVTSRAVGVYGLNGEKIFLWESDKKWPIASITKLMTALVASDLIE
                     REKSIYITAGALSVEGGPGGFSEGESFLMKDLFKPLLVASSNVAAEAMASHYGRERFV
                     AAMNRYAGRLDMNDTLFIDPTGLSINNQSTVADLEKLVSFIYKTKPEIFDITRKKNII
                     ISDARTGKTLQFVNTNLFASLPNFIGGKTGTIPEAVGNLISMFSIEALDSQGIVIILL
                     GSENRFIETQKILNLYGRRS"
     gene            4802..5875
                     /gene="mraY"
                     /locus_tag="VI432_01265"
     CDS             4802..5875
                     /gene="mraY"
                     /locus_tag="VI432_01265"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY4496764.1"
                     /translation="MAEGVSIIGNISLEGARVLATAVISFAVALIVTPLVLQVLKRFN
                     FGKQIRASGKTPIFSELHKGKEGTPTMGGVIIWGTVLFLAGLFFVLDALFDGFFSRLN
                     FINRAETYLPIAALLIAALMGIVDDLLGIFKKGQNSGWGIGIGHKIFIYIMIAVLGAW
                     WFYYVLDWNVLHIPFWGNIDVGWWYIPIFMFIMVASAFSANETDGLDGLLGGVSLFAF
                     SSFAVVAFALERYDLASMSAAVVGAILAFLWFNIYPAKFFMGDTGSMALGITMGMMAM
                     LTNTLFILPFFAIILVIESTSVIIQKISKKFWGRKIFLSTPIHHHFEAIGWPESQVTM
                     RFWIISIVFSGLGLAIFFLDRLS"
     gene            5931..>6247
                     /locus_tag="VI432_01270"
     CDS             5931..>6247
                     /locus_tag="VI432_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY4496765.1"
                     /translation="MSILIKNVEIIDGEGRTPYKSDVFIEKNIISAIGNFKDKEADEV
                     IDGLGNYLAPGFIDIDTASDHYLGIFTYPEQSDFVNQGVSTILGGHCGSSLAPLLYGT
                     LES"
BASE COUNT         1797 a         1009 c         1421 g         2020 t
ORIGIN      
        1 gtagtagcta atgagcttct tttaagttct caatgatagc tacttccaat gggacgattg
       61 ggaatggaga gtaacgcatc tcgctgtaag cgcgaataag ggattttatt aatgtgatgt
      121 gtttttccgg aataatcatt ccgctatgtt ttttaagctc ttttatttcg tcttcggtga
      181 gttcgcgcct gaagatctct tcgatagccg gataaaactt catagataga gcgcgtctga
      241 aataatggat taaatctttg tttaattgga cgatattaaa tccagattca tgaatttcag
      301 ataggtattc cataactgcc ggcaagtcgc cctccagaat ataatgagca agagtcgatg
      361 tgcggataaa accaaccttt cccaaaactc gttctactga ctccagtgtc gcttcttcgc
      421 ctaaagatat tacttgatct aataatgatt ctgcgtctcg caagcttcct tcggaggccg
      481 aggcaataag ctcgattgcg ggttggtcta tctttatatt ttcagcggcg acaactttat
      541 ttaatttttc aacaatcttg ataaccggca gccttctgaa atggagtctt tgggttcgag
      601 aaacaatcgt cggtggcagt ttttcatatt cggttgtggc taggataaag atggcgtgag
      661 atggcggttc ttcaagcatc tttaaaagag cgttgccggc ctctttggtt aactggtgag
      721 cttcgtcaat aataaaaact ttgtattgat agctggatgg ggagagtcga gccccctctt
      781 taagattttt catttcatct attccacggt ttgaggcggc gtcaatctct acgatgtcta
      841 acgctcgtcc ggcttcagtc tccaagcaat gagagcattc gttacatggt tctccctcgg
      901 ttttgaattt ctgatctttt tgtcttgtct cgcaattgac gatttttgca aggattcttg
      961 cggcggtcgt tttcccagta cctctcgggc catagaagag ataggcgtgg ctgattttgt
     1021 cttttctggc ggcatttttt aagacttcca caatcatgtc ctgaccgatt aggtcgtcta
     1081 atattttggg cctatattta cggtatgtcg ccaaactcat agctttcatt atacgtagat
     1141 ataccctaaa atgaaatacg atattatgcg tcgatttttc tatattttat tgccttttgc
     1201 tcttctttcg ggagtatatt tgttgcttgc tcaggaacgc agttttgatg tgaattatga
     1261 cggcataaac gagtttgatc ctgtgttttc agatggggtt gttttagccg ttccggaagc
     1321 ctctattaat gaggttaaag atagtcagat aaagtccgtt aaagcatcag ctgatattcc
     1381 ataccagaaa cagcttgatc cgactccaga gacaataaag gctatttatg taaccagttg
     1441 gtctgccgga aacaagaagg cgatagatag actgattgat cttattagag aaaccgagtt
     1501 gaatgcgatg gttatagata ttaaagatta ttcagggcat gtgacttatg atattgaagt
     1561 cccagacgtt ttaaaataca aagctaagga ggttaggatc tctaaaatta attcgctgat
     1621 taagaaattg catgacgaag gaatctatgt gattgcaagg ttgactgttt ttcaggaccc
     1681 gattttatct tctataaggc cggatttggc tgtgcaaaat atttcaaccg gtgagccatg
     1741 gcttgatcgt aagggtttgg ggtggattga tccctctgcg aaagaagctt gggattataa
     1801 tatcgcgatt gcaaaagatg ctttattgcg cgggtttgac gaaataaatt ttgattatat
     1861 caggtttcca tctgatggcg atttaagcct gatgaagttc cctttttata atattgagac
     1921 tgtttataaa aaggacgcaa tcagagaatt ctttgcatat ttaaggggcg agcttgaggg
     1981 ttataaaatt tctgctgacc tttttggact atcatctata aataaagacg atttaggaat
     2041 aggccaggta attgaatatg cttatgaatt ttttgattat gtaagtccga tggtttatcc
     2101 atctcattat gccaaaggat ttttagcatt agatgagccg gcaaagtatc cgtatgaggt
     2161 aatcaaatat tctatgcata cagcgatggt gcgtttgata gcatatagtc aggacgtcat
     2221 aatgcaggcc ttggacgagt tgcagtctgc gtctggtgga gagtcgccaa aagtgaaatt
     2281 gccgaattca aaattgagac cgtggataca agactttgat cttggaggag tgccctatgg
     2341 ggtagaagaa gtcagggcgc agattaatgc tctatatgac agcgcaaaga gctttgaatg
     2401 tcagggagaa aaccagacgc tgtgtcttaa tccgttgaat ggctggatgc tatgggatcc
     2461 ggcgaatatt tataccaaag aagctttatt atctacggaa taataaattc gaaattttaa
     2521 acaataaaca ataaataata tcaaaaattt aatgaccgaa ataacaaaat taaatatggc
     2581 cggaatttat aaattgaata ttgtaaatta tttgttgtct agatattgta atttgatatt
     2641 tttaatatga atgtatatga gtttgaagta ggtgaattca aggggccgat tgataagttg
     2701 ttggagctta tcgagtctaa aaacatggag atcacaagat tgaatcttgc ttcagtcact
     2761 tcagattttt tagattatat aagaaactta cgcgaagctg attctaaaac tatatctgat
     2821 tttatttctg ttgcgtctca actcattctt ataaaatcgc atgccctctt gcccaattta
     2881 gaattgaatc ccgatgaaga gaaatctata gaagatctgg agaggaggct tgcgacctat
     2941 agagaattca ggcttgttga gaagaaaata gccaagttat gggaagattt tccggttttc
     3001 tccagagaat tcgttttggg agaaggtgga ggattttatt tgagcgatga ggtgactccg
     3061 aggatgcttt tcgaaaagat tggagagctt gtaaaagatt ttcaatccat catgcctaaa
     3121 tatgaatctc ataagataaa actgattaac tttgaagaga aggtgaagga attgataggg
     3181 cgtattgaaa aaacaatatc ttcaagtttt aatgagataa cgaagggcag ggagcgctct
     3241 gaagtgattg tgatgtttct tgcattgttg caccttctaa aggatagcct gattaaaata
     3301 aaccaagaaa acgcttttag cgatatagaa ataactaaaa taaattaatt ttaatagaat
     3361 ggatcaaaat attaataaat taacatcgct tatagaatca ctgctttttg ttcatggaga
     3421 gccgatgaaa ataggcaaaa tagcagagat tataggggtt gatcagggag acgttgaaca
     3481 atgtttaggg attttaaagg accgtcttga tagagaggat agcggaatat cacttatgac
     3541 ttttgacggc aatgcatctc ttatcacaaa gccagaattc ggcagtgtta tacaaaaaat
     3601 ggttaaagag gaaatagata gtcctcttac tccagcgagt cttgagacgc tttctatcat
     3661 cgcatacgtg ggcccaatct ctagagccgg cattgagtat ataagaggag ttaactcatc
     3721 tttcattttg agaagtcttc ttatcagggg gcttattgtt aaagagaccg gattggataa
     3781 gggtagggtt ttatataaag ttacttttga ttttttgaag catgttggtg tgagttcggc
     3841 cgaacaattg ccggagttcg aaaattataa aggaatattg aataacttat taaatccaac
     3901 tgaatcagct ggagatgata ataaaaaatg acgatgaaac ctatttatca agcgatgctt
     3961 ttgaccgtta taattttatt cttcgctctt attaatggag gagaggaggt tggcagcgct
     4021 cttgataatt ctcatactag tttcggcgcg gctagtcttg agggctatag gtccctagat
     4081 gttagtcccg atataaaaga tgtaacttcg cgcgctgtcg gcgtttatgg tttgaatgga
     4141 gaaaaaatat ttctttggga gagcgataaa aaatggccta tagcttcaat cacaaaattg
     4201 atgacagctc ttgtcgcaag cgatttgatt gaaagagaga agtcgattta tataactgcc
     4261 ggggcgttgt ctgttgaagg cggtccgggc gggttttcag agggtgaatc ttttttgatg
     4321 aaagatctat tcaaacctct tcttgtcgcg tcaagcaacg tcgcggcgga agcgatggca
     4381 tcccattatg gacgcgagag atttgtcgcg gccatgaata ggtacgccgg gagacttgat
     4441 atgaatgaca ctctttttat agatcctaca ggtctttcta taaacaatca gtcaacggtc
     4501 gctgatttag aaaaattggt cagctttatt tataaaacaa aaccggagat ttttgatatt
     4561 actcgaaaaa aaaatataat aataagtgac gcaagaaccg gcaaaacttt acagtttgtt
     4621 aataccaatc ttttcgccag cttgcctaat tttattggcg gaaagactgg aacgattcca
     4681 gaggcggttg gcaacttgat atctatgttc agtattgagg ctttggacag tcaaggaata
     4741 gttataattc ttttgggttc agaaaacaga tttattgaaa cgcaaaaaat attaaattta
     4801 tatggcagaa ggagttagca ttattggaaa tatttcgctt gaaggcgcta gggttcttgc
     4861 aacagccgta atatcttttg ctgttgctct tatagtaact ccgcttgttc tccaggtttt
     4921 gaagagattt aattttggca agcagataag agcaagcggt aaaactccta ttttttcaga
     4981 acttcataaa ggaaaagagg gtacgcctac gatgggagga gtgataatat gggggacagt
     5041 cttattttta gccggactat tttttgtttt agacgctctt tttgacggat ttttttcaag
     5101 attaaatttt ataaacagag ccgaaaccta tcttccgatt gctgcgcttc tgattgccgc
     5161 cttgatgggc attgtagatg accttttggg aatttttaag aaggggcaga acagcggatg
     5221 ggggatagga ataggtcaca aaatttttat ttatattatg atagctgtcc ttggggcttg
     5281 gtggttttat tatgttttag actggaatgt tcttcatatt ccgttctggg gaaatattga
     5341 tgttgggtgg tggtatattc ctatttttat gttcattatg gttgcaagcg cattttccgc
     5401 gaatgaaacc gatggactag acggacttct cggaggagtt tctcttttcg ctttcagctc
     5461 tttcgcggtc gtcgcctttg ctttagagag atacgacctt gcgtctatga gcgcggctgt
     5521 tgtcggcgcg attttggcat ttttatggtt taatatatat cctgctaaat tttttatggg
     5581 agatacgggt tcaatggctt tgggtataac gatgggtatg atggctatgc ttacaaatac
     5641 cttatttatt cttcctttct tcgcaataat tttggttatt gagtctacgt cggttattat
     5701 ccagaaaata agcaagaaat tttggggaag aaagatattt ttatcaacgc ccatacatca
     5761 tcattttgag gctatcggct ggccggaatc tcaagtgaca atgagatttt ggattatatc
     5821 aattgttttc tcgggtttgg gattggctat attcttttta gaccgtttgt catagcgatg
     5881 tagagttgcc ggttggtgta taatataact tggttatatt tataaacgct atgagtattt
     5941 tgattaaaaa tgttgagatt atagatggcg agggcagaac tccttataaa tcggacgtct
     6001 ttatagaaaa gaatattatt tcggcgattg gcaatttcaa agacaaggag gcggatgagg
     6061 taatagacgg actcggcaat tatttagcgc ccggttttat agatatagac acagcgtcag
     6121 atcattattt aggcatcttt acttatccgg agcagtcaga ttttgtgaac cagggtgttt
     6181 ctacaatact cggcggacac tgtggatcat ctcttgcacc gcttttatat ggaacacttg
     6241 aatcaat
//
LOCUS       DATDNZ010000032         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1919203, whole genome shotgun sequence.
ACCESSION   DATDNZ010000032 DATDNZ010000000
VERSION     DATDNZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919203"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VI432_01275"
     CDS             <1..298
                     /locus_tag="VI432_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4496766.1"
                     /translation="HTDTQAMVLIEAAASGLPIIALDDGAFAGTTVLDGVNGRLVSPD
                     DTGIFADEALRILNDDKAYEKLRNGSLKLSLDFSQESQAAKLIDIYNRLIAEDQ"
     gene            295..813
                     /locus_tag="VI432_01280"
     CDS             295..813
                     /locus_tag="VI432_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY4496767.1"
                     /translation="MIVVLHNIRSIHNVGSIFRTADALGVKKIYLTGVTPSPLDEFGR
                     SRQQFIKVSLGAEKTVDWERVISPVKLINKLEKDGFLFFAVEQSDKSIPYFKLHRRYK
                     FNKIVLVLGNEVKGLSSAVLNKCDKILEIPMKGAIVRQAHHPRNIGSGKESLNVAISF
                     AIVGYHLVYIGG"
     gene            870..1856
                     /locus_tag="VI432_01285"
     CDS             870..1856
                     /locus_tag="VI432_01285"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HEY4496768.1"
                     /translation="MSELRQDPVSGDWMINAPERAKRPHDFLPKRKKRKSTPKSRCPF
                     EDLKRSGNWPPIVLVTNKNEDDWRAVIIPNKYPALMHKRSCATTSYRGPYKLLSGIGH
                     HDVLVSRDHNKNIAHLNLRDGMAIFRALQKRYKMLIADSCMVYASAFMNWGEGAGASL
                     HHPHFQILTLPITPPDVEHSLRGSQDYFRKNNKCVHCEMIGDEVKSKKRVILKNKYAV
                     AVAPFVSRQPFEVRIYPLRHRPYFERVKDGELESIVDLLQRTLLRVEKKLNDPDINFF
                     IHTAPVQYQNRYKHYHWHIEILPKITVQAGFEKGTGVDINVIDPNKAAELLR"
     gene            1938..>2983
                     /locus_tag="VI432_01290"
     CDS             1938..>2983
                     /locus_tag="VI432_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496769.1"
                     /translation="MIDSEKIDRGVRKKVVELASSLNSNIYLEFRPLTSREFFKSLRK
                     KIEHTYILYDELVGAVKEIRHFVHETEWILDNFYILEGTVSDIVSSLHDYRKEVGFIP
                     YLTEKDVFFGAISYPRIYYIASELLKVTNNKIDWSTLRVFIAEYQKKAPLSIRELSFL
                     HTILRILLIENAQKLIEQSINSFDEFKEAKDWLARIEKEIKRNKNPDFSKLISSLSSE
                     YQLVPIDFAFYLHQHLGRYGPSARTIVRWLKLNLAKQKVDIKDLAEIEAKQRNSNSVF
                     IANIINSLRWLNQARWDDFVDQTNSIDPILKKDPAGVYKQMDSKSQDFYRRAIMKMAD
                     RTGIYESEISKIAI"
BASE COUNT          963 a          465 c          638 g          917 t
ORIGIN      
        1 gcatacagat actcaagcca tggttttaat agaggcagca gctagtgggc tgccgataat
       61 agccttggat gatggagctt ttgcaggaac aacggtttta gacggtgtga atggacgcct
      121 cgtctctccg gatgatacag gaatttttgc agatgaagct cttcgaatat taaatgacga
      181 caaagcttac gagaaattaa gaaatggatc actgaaatta tctttagatt tttcgcaaga
      241 gagccaggcg gctaaactta tcgatatcta taatagattg attgccgaag accaatgata
      301 gttgtcctcc ataacataag aagcatacat aacgtcggat cgattttcag aaccgcggat
      361 gcgcttggcg ttaaaaagat atacttaact ggagttactc cgtctccttt agatgagttt
      421 ggcaggtcaa gacagcaatt cattaaagtt tctttaggag ctgaaaagac agttgattgg
      481 gaaagggtta tatcgcctgt taaattgata aacaagcttg aaaaggatgg ttttttattt
      541 tttgccgttg agcaatctga taaatcaatt ccttatttta aacttcatag acgttataaa
      601 ttcaataaga tagttctcgt tctgggtaat gaagttaagg gtttatcatc ggcagttttg
      661 aataagtgcg ataaaatttt agaaattcct atgaaggggg caatagttag gcaggctcac
      721 catcccagaa atattggcag cgggaaagaa tctttaaatg tagccatatc ttttgcgatt
      781 gttggatatc atcttgtcta tattgggggt taaatattat atagggatga tccttattat
      841 tcgctcgcat ttttttgtat aattttaata tgtcagaatt gcgtcaggat cctgtttcag
      901 gagattggat gatcaatgcg cccgagaggg cgaagcgccc gcacgatttt ttaccgaaaa
      961 gaaagaagag aaagtctacc cccaaaagta gatgtccttt tgaagatctt aaaaggtctg
     1021 gcaattggcc accaatcgtt ttagttacca ataaaaatga agacgattgg cgtgcggtta
     1081 ttattccaaa caagtatccg gcgttaatgc ataaacgttc atgcgccaca acttcataca
     1141 gaggccctta taaattattg agtgggattg ggcatcatga tgtgcttgtt tcgcgcgatc
     1201 ataataaaaa tatagcgcat cttaatttaa gagacggtat ggctatattt agagcattgc
     1261 agaagagata caaaatgtta attgctgata gttgcatggt ttatgcatct gcttttatga
     1321 attggggaga gggcgccgga gcttctttgc atcatccgca ttttcagata cttaccttgc
     1381 ccattactcc gcctgacgtt gaacattctt tgcgggggtc tcaggattat tttcgtaaaa
     1441 ataataagtg cgttcattgt gagatgatag gtgatgaagt taaatctaaa aaaagagtta
     1501 ttttaaagaa taagtatgcg gttgctgtag ctccatttgt ttcgcgtcag ccctttgagg
     1561 tgcgaattta tccattgagg caccgtcctt attttgagag agtaaaagat ggcgaattgg
     1621 agtctatagt agatttattg cagagaaccc ttttaagggt agagaagaaa ttaaacgatc
     1681 cagatattaa tttttttatt catacagcgc cggtgcaata tcaaaatcga tataagcact
     1741 atcattggca tatagagata ttgcctaaaa ttactgtgca ggcgggtttt gaaaaaggaa
     1801 caggcgttga tattaacgtt attgatccga ataaagctgc tgagcttttg cgataagaat
     1861 gtctagttag taatcaggtt attcaattat tcggcctttt atcaccaagc ataaattaag
     1921 ctatacttaa tttattaatg attgatagcg agaaaataga tagaggtgtg cgtaaaaagg
     1981 tggtggaatt agcctcttct ttaaatagta atatatattt ggaattcagg cctttgacat
     2041 ctagggaatt ttttaaatct ttaagaaaaa agatagaaca cacgtatatt ttatatgatg
     2101 agttggtcgg cgcagtcaaa gagataaggc attttgtcca cgaaaccgaa tggatcctgg
     2161 acaactttta tattttagaa ggaacagtat cggatatcgt atcaagcctg catgattata
     2221 ggaaagaagt cggttttatt ccgtatttaa ccgaaaaaga cgtttttttt ggagccatat
     2281 cttatccaag aatatattat attgcctcag aattattaaa ggttacaaat aataagattg
     2341 attggagcac ccttcgtgtt tttatcgctg aatatcagaa gaaagctccg ctttcaataa
     2401 gagagctttc tttcctgcat acaatcttga gaattttact tatagagaat gcgcaaaaac
     2461 tgattgaaca atcgataaat tctttcgatg aatttaaaga agcaaaagat tggcttgcca
     2521 gaatagaaaa agagattaag agaaacaaaa acccagactt ttctaaatta atatcaagtt
     2581 taagcagtga ataccagttg gtgccgatag attttgcttt ttatcttcat cagcatttgg
     2641 gccgttatgg tccttctgcg cgtaccattg ttagatggct taagcttaat ttagccaagc
     2701 agaaagtgga tataaaagat ttagctgaaa tagaggcgaa gcagaggaac tctaattccg
     2761 tttttatagc aaatattata aatagtttaa gatggctgaa tcaggcaaga tgggatgatt
     2821 ttgtagatca gacaaacagc atagacccaa tccttaaaaa ggatccagcc ggagtttata
     2881 agcagatgga ctctaaaagc caagattttt ataggagagc cattatgaaa atggctgacc
     2941 ggacaggtat ttatgaatct gaaataagca aaatagcaat taa
//
LOCUS       DATDNZ010000033         2935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_91218, whole genome shotgun sequence.
ACCESSION   DATDNZ010000033 DATDNZ010000000
VERSION     DATDNZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91218"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VI432_01295"
     CDS             complement(<1..718)
                     /locus_tag="VI432_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496770.1"
                     /translation="MSNVLIEKLRDKLIELSHFGCSLSLLGWDQEVNMPPKGADKRAD
                     TIAYFTGLAHQKLKEIDKDKTLTKLIKLMDADKLSAGEKAVVREVKRGFDKMIRLPDS
                     FIKELAGTASKAQSVWVEARHKSDFDMFLPWLKKIITLKRKEAELIGYKFSPYDALLD
                     TFEPELTAEDTSRILNDLKDFLVPFIEQIRKSKSKIVKKLGEGKFAVDKQIEFNKLIA
                     LKMGFDIDAGRIDKSAHPFTT"
     gene            805..1392
                     /locus_tag="VI432_01300"
     CDS             805..1392
                     /locus_tag="VI432_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4496771.1"
                     /translation="MKQVLIIGGGDSFKNHRVYLKFLKNFKIDLKKYRAGKHDWKENL
                     QKKLGNNFDVFLPTMPNRTNAKYSEWKIWFNKLIPQIKSGVILIGHSLGALFLIKYLS
                     ENKFPKKIRAVFLMAPPYNEGSFSRPRNFRQLEKQAGKLFIYYSEDDKIVSIKDIEKY
                     KKNVLSAIYKIFEHQGHFNKRSIPGLSREIKKLYK"
     gene            complement(1827..2741)
                     /locus_tag="VI432_01305"
     CDS             complement(1827..2741)
                     /locus_tag="VI432_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY4496772.1"
                     /translation="MATALYRIIKYGLKNFWRQKLVSLATLTIILLAILVFEGLVVFN
                     VVAKQALILIQDKIDISVYFKTNVAEDEILQIQRSLEDLEEVRSIDYVSRDEALEIFN
                     TTHAGDEDGVVLQALEKLDENPLSASLNIKAERPSEYPIIAGYLDKNISPELVQNISY
                     NQNKVAIDRLTSIISVSKSTGIILTIILSIVAIIVAFNTILLAIYSNREEISVMRLVG
                     ASNTFIRGPFIVEGIIYGFLAAFISIFITMPVIYFASPSLNSLIPGINIFSYFISNIF
                     QLFFYQLLFGVTIGVISSLIAIRRYLKI"
BASE COUNT          987 a          589 c          479 g          880 t
ORIGIN      
        1 ctgtcgtaaa tggatgcgcg cttttatcta tccttccggc atcgatatca aaacccatct
       61 tcagggcaat caacttatta aattcaatct gcttatctac ggcgaatttc ccttctccta
      121 attttttaac tatctttgat ttagattttc taatttgctc gataaaaggc accaagaaat
      181 cctttagatc attgagtatc cttgatgtgt cttcagcagt taattcgggt tcaaaagtat
      241 ctaacaaagc atcgtatggc gaaaatttat aaccaataag ctctgcttct tttcttttaa
      301 gggtaattat tttctttagc caaggcaaaa acatatcgaa atctgattta tgccttgctt
      361 caacccatac gctttgagcc ttagaagctg tcccagctaa ttccttaata aatgagtctg
      421 gcaaccttat catcttgtca aaacctcttt tgacttccct caccaccgcc ttctcgcctg
      481 cactcagctt atcagcatcc ataagcttaa ttaacttagt aagagtcttg tctttatcta
      541 tttctttgag tttttgatgc gctagtccgg taaaatatgc gattgtgtct gctcgtttgt
      601 ctgctccttt gggcggcata tttacctcct ggtcccaacc aagcaatgag agagaacaac
      661 caaagtgaga cagctctatc aatttatctc taagtttttc aataagtacg ttgctcatat
      721 acagattatt atataaatct tccagattaa tacaacgagt gtaatcaata atataatctt
      781 gacttcatat gctatattta agcaatgaaa caggtattaa taattggggg cggagacagc
      841 tttaaaaatc atagggttta tctaaaattc ttaaagaatt ttaaaattga tcttaaaaaa
      901 tatcgcgccg ggaaacatga ttggaaagaa aacctgcaaa agaaacttgg aaataatttt
      961 gacgttttct taccaacaat gccaaataga acaaatgcaa agtattctga atggaaaatt
     1021 tggtttaata aattgatacc ccaaatcaaa tcaggcgtaa tattgatagg ccactccttg
     1081 ggagcgctat tcttaattaa gtatttatct gaaaataaat tccccaagaa aataagagca
     1141 gtttttttaa tggctccgcc ctataatgag ggcagcttca gccggcctag aaatttcagg
     1201 caattagaaa aacaggctgg caaattattt atctattaca gcgaagacga caaaatcgta
     1261 tccatcaaag acattgagaa atataaaaag aatgttctct ctgcaattta taaaatattt
     1321 gagcatcaag gacattttaa caagcggtct ataccgggac tttctagaga aatcaaaaaa
     1381 ttatataaat aaatttgaca caagaataaa atatattaat attcttatca gctcttaaat
     1441 gttattggcc gtttgatgcc cttgcaatac ttcactcaga caaaaatggg taagcgttta
     1501 acgcctactc catcctcccc ctgcattaat gtatgattgg aagtaacgaa cacggcagaa
     1561 agaggtctcg caaatgactc ctcgcctaag gaaggcggag aggatggaag tagggcgcat
     1621 cccacccatc caacctcctg caatcgtgtt tgtaaccggt tcaaatccgg tcaagggcat
     1681 acaaatcaac gcgaccccaa aaacatttgc cgccacaaaa aatgcgcatc cccagagaaa
     1741 cataaagggc ggcagacaac caaggcccca gagtgagaat ccccatctca ctctggggcc
     1801 acttttttat taaaaactcc ttctgattaa atttttaaat atcttctaat agcgatcaag
     1861 cttgaaatca ctccgatagt cactccaaat aacagctgat agaaaaatag ctggaaaata
     1921 tttgaaataa aataactaaa aatatttatt cctggtataa gagaatttaa agaaggcgaa
     1981 gcaaaataga taaccggcat agtgataaaa atacttataa aagccgccaa aaatccatat
     2041 attattccct ccacaataaa tggcccgcgt ataaaagtgt tagacgcccc aacaagcctc
     2101 ataacgctga tctcttccct gtttgaataa atggcaagaa gtatagtatt aaaagcgact
     2161 attatggcca cgatcgaaag aatgatcgtc agaatgattc cagtgctttt actaacacta
     2221 ataatagaag tcaatctatc aatcgctacc ttattctgat tgtaactaat attctgcacg
     2281 agttctggag aaatattttt atcaagataa ccggctatta ttggatactc tgatggcctt
     2341 tcagctttaa tgttcaaaga agcagaaaga ggattttcat ctaatttttc caatgcctgc
     2401 aataccacgc catcttcatc gcctgcatga gtggtgttaa aaatctcaag agcttcgtct
     2461 cgcgaaacat aatcgataga tcgaacttct tccaagtcct ctagagatct ctgaatctgt
     2521 aaaatttcgt cttcggcaac attagttttg aaataaacgc ttatatctat tttatcctga
     2581 atcagaatca aagcctgctt tgcaacaaca ttaaaaacga caaggccctc aaaaaccagg
     2641 atagcaagaa gaataatcgt taacgtggcc aaagatacga gcttctgacg ccaaaaattt
     2701 tttaaaccat attttattat gcgatataga gcggttgcca tgctaatgat gtttaatttt
     2761 tcaaggtctg ctgtagcaaa gaaaaattaa ctacaacatt cagcacccgc ctaaattttt
     2821 gcaaattaat cattgagtat attgtataat attttgttta tatatctacg cccatatccg
     2881 gttttaaaat attgctctct atatattgca aaaacttggg cgggtaagat tttat
//
LOCUS       DATDNZ010000034         8728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1156609, whole genome shotgun sequence.
ACCESSION   DATDNZ010000034 DATDNZ010000000
VERSION     DATDNZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156609"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VI432_01310"
     CDS             complement(<1..269)
                     /locus_tag="VI432_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496773.1"
                     /translation="MKTARAVRLEDGVIPRLKEESVTFFTPWGPRYDYEVRGSEIEDS
                     HREIDLLKFLARILGEFKKNMPGRKFRWIFLGADLYGVKINHLPQ"
     gene            complement(387..1889)
                     /gene="hutH"
                     /locus_tag="VI432_01315"
     CDS             complement(387..1889)
                     /gene="hutH"
                     /locus_tag="VI432_01315"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY4496774.1"
                     /translation="MDIKVGPSNKINYSVVYEVAFNPKVKISLSPRAYASVKKSRNVI
                     ERMLEKGKVVYGVTTGFGNFKNKFISKEDAAELQVNLIRSHSTGVGPPLSKEAVRAAL
                     FVRLNSLVQGYSGVRVELLELLCGLLNKDIIPIVPSQGSVGSSGDLAPLSHMGLVLMG
                     EGEALYKGRIMPGGEALKRASLKPITFTAKEGLAWNNGTSVMTGIASIVLYRANKLIN
                     IADMASALTLEAMCGVSSHLDQRIHNIRPHPGQKISAGNIKKFIKGSKLVDSAPDRIQ
                     DAYSIRCAPQVHGAVRDAFQYVADVVNREVNSVTDNPLIFGDPDRAISGGNFHGEPIA
                     IAMDTLSIAMSELANISERRTAKLVDSSTNEGLPMFLIDGEKAGLHSGFMISQYTAAA
                     LVSENKVLAHPASVDSIPTSANQEDHVSMGTIAARKAMTISENVENVLAIEVMTACQG
                     IDFRGKYKLGYETKRFYSAVRKMVSFLSGDRVLSEDIINTKRAIDSLFLD"
     gene            complement(1894..2301)
                     /locus_tag="VI432_01320"
     CDS             complement(1894..2301)
                     /locus_tag="VI432_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496775.1"
                     /translation="MHQPPLILTVDDQADFRDIIKARLEAAGFRVETAVDGEVGIKKA
                     KELKPDLVLMDVKMPGINGTEAVLDMQNDPDLKDIKVVFLSNLAAPWPGVSAPNEEFA
                     KILGAHTFIDKSKELENVAEHVKKILGVDPKKS"
     gene            complement(2270..2680)
                     /locus_tag="VI432_01325"
     CDS             complement(2270..2680)
                     /locus_tag="VI432_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4496776.1"
                     /translation="MANKPLILFVDDSEDFRDIFSTKLTAEGFDVVTAVNGEDALEKL
                     KNINPEVILLDLEMPVMNGVETLLKIRSDDKFKNAKVLFCTNYGEPKERAAWTDEKFA
                     KEIGAIGYLRKTDDLDTIAKQVKAYASTSININR"
     gene            complement(2774..4549)
                     /locus_tag="VI432_01330"
     CDS             complement(2774..4549)
                     /locus_tag="VI432_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4496777.1"
                     /translation="MKKTKIGGHLRDKILIMMFVVGIFPVVIAGFFALYSVTRAHNLN
                     VASIEENLINQKTEEIRAFIGGIISILEIKTPFEQTGDIAYEDQIFILNGLLSERGAA
                     EEASFINLSSGIETSRVGREHIRLDRVPELRDQSNLQKFLTAKEGSVYVGPVYSTEGG
                     SRTSVAVPVRNKNDVVISVLSAEINLDQLQSIASATKLGNTGYLYIVDESGRIVASSL
                     EKIAEIDLEKLPIIRALINGQSFLGYEDQERYQGIRGERVIAAARFMEDLNFGIVAEW
                     PVSDADLVLESLRNQIIMFSILALIAILILSFILSKAIVRPVKKLEEGTKLVAQGKFD
                     VSIDIKTGDELEDLGKSFSKMTEGLKQLQALKDEFVFIAAHELKTPVAAIKGYLSLIT
                     DGFAGEVNDKVKEFINKVNKSNARLITLVEDLLEVARSEAGRLQIAVQPVDIIEPIKD
                     TLSELKSLSDEKSIEVVYNPPDATLKVMGDINRIKEVMVNLVGNAIKYTLGKGMITIN
                     HEVKDGKLVTRIKDTGLGMSKEAQAKLFQKFYRVQTKDTANISGTGLGLFIVKQIIEK
                     MNGSIWVESEEGKGSTFSFSLQIAK"
     gene            complement(4559..5308)
                     /locus_tag="VI432_01335"
     CDS             complement(4559..5308)
                     /locus_tag="VI432_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496778.1"
                     /translation="MKSNILLALGAILLIGTAYLLFIDSAKYNNPSIGIDPHVYGDKS
                     KGIAKIKLYVFYAVPNNKTAGDLSLIQSKSIDVLEEVSSFHKNQFRGESLIEYYIYPE
                     VVFLENDNLAYDTYSTQYGNFEALKSITLELENRAFKPEGDLSQNLLGVDSEGAFRVI
                     AIIYEGVGASGTEGSMILSRDFMTRDEYSLFRPSLFYHEFGHTIGMPEGYDIKTNRPF
                     TNDIMGAGRREPIRTNFIESETLKEMGVISE"
     gene            complement(5317..8478)
                     /locus_tag="VI432_01340"
     CDS             complement(5317..8478)
                     /locus_tag="VI432_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY4496779.1"
                     /translation="MIYTNKFLILFIIASLFWLYVPISLASAPPEITNVSTSTTTTTA
                     DIFWRTDNHLSDSKVDYAIHPNSPNIQVSNSCPGYSNAATPTTEHCIRITGLQEGKRY
                     NFKAKSFGIYGINQSGTGEYDDEFTTKDNTPPTNVNLTLPGDNATVSGTAEAAATATD
                     SGSGVSEVVFNISNSTNSYDIGSNPTPSGGDTYHINWNSALYPNGSYTLKATAYDSAT
                     PRNQTASAGRTITISNTIGGQGQPDTTAPTAPTNLSGTPSSNSVQLSWSGSTDNVGVT
                     GYEIHRGSALGLNFQLIETVSALSFSNSGLSANTTYTYKVRAKDDAGNLSSFSNTITV
                     VTLGSGENQPPSVPQNVSASPESTTSIKITWNASTDPENNTISYTIYRGTSELTSVNG
                     TSYTDTGLSPSTSYTYKVRAKDSFGALSGFSDAAEATTNSSPQPTPPPPPPGQTGDAK
                     TISGKVIFSNNSAVTDAEVKIYNSSTGSYMTALSSFNGSYSFTVSGGQWTVNIAPLAS
                     SNWTWSEPIQTVSFADNSLSEQRTVDFTVPLANAKLSVKVLGPDGRALVGAGVIIDST
                     SAKDRALGLYSGTTVVGVSDQDGLVSFNLSPGTFYIRARSSEVGFTQPQEKSAQVFSN
                     TETATYIQFGSVKAFITGTARLADGRPTDALIWGWSNDGRTVQTRATSAGQFSLPVST
                     DLRWRVGASKLEGKTPYRSAEIVVDIRGASVTLGNLVLNAEDITLPDQVSQTTNIQSG
                     SVINLTDRAKIEIPADLVSLAGDLTVSISPTVEVPVQFNNIVGAVYDIDLKDKDGVAI
                     TNFNKKITLSFPYTVKLLAGLGITADQLVPSFYDDESGLWVRVADYYVDKLNTRVVAS
                     FSHLTRFALVSAADIIPPAPPTQVGAFKTGVNVLLQWVNPKEDFRHTKIYRSAQKGIL
                     GSVLFPEVLGSSRVDATVSPGVMYYYVIRSVDPAGNESTNIDQIVPSDGTPKSIHASS
                     ISRNLRRGMSGTDVSLLQQTLVTEGVYPEALVTGYFGPLTHAAVVRFQEKYFSEILTP
                     VGLNKGSGFVGPSTRAKLSAVIN"
     gene            complement(8527..>8728)
                     /locus_tag="VI432_01345"
     CDS             complement(8527..>8728)
                     /locus_tag="VI432_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496780.1"
                     /translation="FTSGAIALYWSYNQDIDHLTIKDRLLQRTDKIVSMTGTSMSGGR
                     LNVCNLFGDTLCSSSVVPIPNL"
BASE COUNT         2560 a         2014 c         1492 g         2662 t
ORIGIN      
        1 tcttgtggca gatgattaat cttgacccca taaagatctg cgccaaggaa aatccagcgg
       61 aatttcctgc ccggcatatt tttcttaaac tcaccgagta tccgagccaa aaatttaagg
      121 agatcaatct ctctatgaga atcttcaatc tctgatcctc ttacctcata atcgtaacga
      181 ggtccccaag gagtaaaaaa tgtcacagac tcctctttaa gacgcgggat aaccccgtct
      241 tcaagcctta cggcgcgagc cgtcttcatc tcaggataaa attgggtatt aaaccatttt
      301 atcccatcat ttagttgtgc cagcaatcaa tgcacctctt tcgattttta acgatccttg
      361 cataaagggt accctgatgc ctaaattcag tcaagaaata aagaatctat agctctttta
      421 gtattaatga tatcttctga tagcactctg tcaccagaca gaaacgaaac cattttacga
      481 acggcggaat aaaacctctt tgtttcgtag cccaatttat atttgcccct gaaatcaatc
      541 ccctgacagg cggtcattac ctcaattgct aaaacgtttt caacgttttc agaaatagtc
      601 attgcctttc ttgcggctat cgttcccata cttacgtggt cctcttgatt cgcagaggta
      661 gggatcgaat caactgatgc tggatgcgca agaaccttat tttcagagac taatgctgca
      721 gctgtatatt gcgagatcat aaatccgcta tgcaatccgg ctttttctcc gtctattaaa
      781 aacatgggaa gcccttcgtt agtggaagaa tcaactagct tggctgttct tctctctgag
      841 atattagcaa gctctgacat ggcgatagat aatgtatcca tagcaattgc gatcggctca
      901 ccatgaaaat ttcctccgga tatagctcta tctggatcgc cgaatattaa tgggttatca
      961 gtcacagaat taacctcgcg atttacaacg tccgcaacat attgaaaagc gtctcttacc
     1021 gcgccatgga cctggggcgc gcatctaatt gaatacgcgt cctgaatcct gtcaggagca
     1081 gaatcaacta atttagatcc ttttataaac ttttttatat ttccggctga aattttttgc
     1141 cctggatgag gtcttatatt atgtatcctt tgatctaaat gactgcttac tccgcacata
     1201 gcctctaggg tcagggctga ggccatatca gctatattta taagcttgtt tgctctataa
     1261 agaacaattg acgcaatccc agtcataaca gatgttccat tattccatgc taatccctct
     1321 ttggcggtaa atgtaattgg cttcaagctg gcgcgcttta aagcttcgcc gccaggcata
     1381 atccttcctt tatataaagc ctctccttct cccattaaaa ccaggcccat atgcgacaaa
     1441 ggagctagat ccccagatga cccaacggat ccctgagaag gaacaatagg aataatatct
     1501 ttattcaata aaccacataa caactcaagt agttcaactc taacgccaga atatccttgc
     1561 accaaagagt ttaatctaac aaacaaagca gccctcactg cctccttgga tagtggcgga
     1621 ccaaccccag tcgaatgact ccttattaaa ttaacctgca attccgccgc gtcttccttg
     1681 ctaataaatt tatttttaaa atttccaaat ccggttgtaa caccataaac gacttttcct
     1741 ttttcaagca ttctttcaat aacatttcta gactttttaa cgctagcgta agcccttgga
     1801 gacaaagaaa tcttaacttt tggattaaat gcaacttcgt aaacaacgga gtaattaatt
     1861 ttattgctag gtccaacttt aatatccatg aatttaagat tttttaggat ctactcctaa
     1921 gattttttta acgtgttcag caacattctc caattctttg gatttatcaa taaaagtatg
     1981 agcgcctaaa atcttcgcaa attcctcatt gggagcggaa actccaggcc aaggagcagc
     2041 cagattgctc aaaaacacaa ccttaatatc tttaagatcc gggtcattct gcatatccaa
     2101 aaccgcttcc gtcccattta ttccgggcat tttaacatcc atcaaaacca aatcgggctt
     2161 cagctcttta gcttttttga tgcccacctc gccgtcaacc gctgtttcaa ccctaaaacc
     2221 agccgcctct aaacgcgctt taataatgtc tctgaaatcc gcttgatcat caacggttaa
     2281 tattaatgga ggttgatgca tatgctttta cctgttttgc tattgtgtct aaatcgtctg
     2341 tcttccttaa ataccctata gcccctattt ctttggcaaa tttttcatca gtccaagcgg
     2401 cacgttcttt aggttcgccg taattagtgc agaacagcac ttttgcgttt ttaaatttat
     2461 catctgatct tatctttaaa agagtctcga ccccattcat aaccggcatc tcaagatcta
     2521 ataaaatcac ttctggatta atgtttttta atttctcaag cgcgtcctcg ccgtttacag
     2581 ccgtcacaac atcaaaccct tcggcagtta actttgtaga aaaaatgtcg cgaaaatctt
     2641 cgctgtcatc tacaaataaa attaatggtt tattcgccat gattattagt ataaggcatt
     2701 acgtataaag tataaagtat tgcaagaaac agcccatact taatacccga tgctaaatat
     2761 tttatgctta atactattta gcgatctgta aagagaagct aaacgttgat cctttgccct
     2821 cttctgactc tacccaaata gatccattca tcttctctat aatctgctta acaatgaaaa
     2881 gtccaaggcc ggtcccggaa atatttgccg tatctttagt ttgaacccta taaaacttct
     2941 gaaacaattt tgcttgagcc tctttcgaca tcccgagccc cgtatcttta atgcgcgtaa
     3001 caagctttcc gtctttaacc tcatgattga tggttatcat acccttaccc aaggtatatt
     3061 taatagcgtt tccaacaaga ttaaccataa cctctttaat gcgatttata tctcccatga
     3121 cttttagcgt agcatctggc ggattataaa cgacttcaat gctcttctcg tctgaaagag
     3181 atttaagctc gcttaatgta tctttaatag gttcaatgat atctaccggc tgtaccgcaa
     3241 tctggagcct gccagcttcg gaccttgcta cttcaagcaa gtcttccacc aaagtaataa
     3301 gccttgcgtt tgatttatta actttattaa taaattcctt aactttatca tttacctcgc
     3361 cagcaaaacc gtcggttatc aaagacaaat atccttttat tgcggcaact ggagttttta
     3421 attcgtgagc cgcaataaag acaaattcat ctttcaatgc ttggagctgt tttaaaccct
     3481 cagtcatctt agaaaaagat ttgccgagat cttcaagctc atcccccgtt ttaatgtcaa
     3541 ttgagacgtc aaattttcct tgcgcaacca attttgttcc ctcttccaat tttttaaccg
     3601 gacgcacaat agccttagag agaataaagc ttaatataag tatcgctatt aaagctaaaa
     3661 ttgaaaacat tataatctga ttccttaaac tctctaaaac caaatcagcg tcagaaacag
     3721 gccattctgc gactatccca aaatttaaat cttccataaa tctagcagcc gctattactc
     3781 tctcccctct aataccctga taacgctcct gatcttcata acccaaaaaa ctctgtccat
     3841 ttataagagc tcttattata ggcaattttt ctaaatcaat ttctgctatc ttctccaagc
     3901 tggacgcaac tattcttccg gattcatcca ctatatataa gtatcccgtg ttccctaatt
     3961 tagtagctga agcaatagac tgaagttgat ccaaattgat ttctgcgctc agaacagaga
     4021 taacaacatc atttttattc cgaaccggaa cagcgacgct agtcctagag cctccttcag
     4081 tagaataaac cggtccgacg tatacgcttc cttcctttgc tgtcaaaaat ttctgcagat
     4141 tactctgatc tctcaattcc ggaactctat caagcctgat atgttccctg ccgactcgcg
     4201 aagtctctat accagatgac aaattaataa acgatgcttc ttctgcggcc cccctttcac
     4261 tcaaaaggcc atttaaaata aagatctggt cttcatatgc tatatctcca gtctgctcaa
     4321 acggggtttt aatctctaaa atgcttatta ttccgccgat aaaagctctt atctcttctg
     4381 ttttttgatt aattaaattt tcctcgatac tcgcaacatt aagattgtga gctctcgtta
     4441 cgctatacaa agcaaaaaaa ccggcgatta caaccggaaa gatgccgaca acaaacatca
     4501 ttatcaaaat tttatcgcgg agatgtcctc ctattttagt tttcttcacc tacttatttt
     4561 actccgaaat aactcccatt tcttttaagg tttccgactc tataaaatta gtcctaatcg
     4621 gctctctcct tccggcgccc atgatatcgt tggtgaacgg cctattcgtc ttgatatcat
     4681 agccctcagg cattcctatt gtatggccga attcgtgata aaaaagagac ggcctaaaaa
     4741 gtgaatactc atccctcgtc atgaaatctc ttgataaaat catgcttccc tctgttccgc
     4801 ttgcaccaac gccctcatag ataatagcaa tcactctaaa agcaccttcg ctatcaactc
     4861 ctagcagatt ttgagaaaga tcgccttctg gcttaaacgc cctattttct aattccaaag
     4921 tgatggattt taacgcttca aagttgccat actgggtgga atacgtgtca tatgccaagt
     4981 tatcattttc aagaaaaaca acttcaggat aaatataata ttctattaaa gattctcctc
     5041 taaattgatt tttgtggaat gaagaaacct cttctaaaac atctattgac ttagactgaa
     5101 ttaatgaaag gtcgccagcc gttttattat tcggcaccgc ataaaaaacg taaagtttta
     5161 ttttcgcaat acccttactc ttatcgccat agacatgagg atcaatacct atacttggat
     5221 tgttatattt agcagagtca ataaacaaaa ggtaggctgt ccctattagt aatatcgctc
     5281 ctagggccaa caatatatta ctcttcattc cgaaatttaa ttaatgacag cgctaagctt
     5341 tgctcttgtt gaagggccaa cgaagccaga acccttattt aatccaactg gagtcaaaat
     5401 ctcagaaaaa tatttctctt ggaatctaac aacagccgca tgagtaagcg gaccaaaata
     5461 acccgtgact aaagcttcgg gatagacccc ttccgtaacc aaagtttgtt gcaacaaaga
     5521 gacgtcagtt ccgctcattc cacgtcttaa atttctacta atgcttgaag catgtattga
     5581 tttcggagtt ccatcagacg gaacaatctg atcaatatta gtcgactcat taccagccgg
     5641 gtcaacggat cgtataacat aataatacat aactcctggc gaaacggtcg catccaccct
     5701 tgaagatcca agaacttcag ggaacaaaac cgaacccaat attccttttt gagcgcttct
     5761 ataaatttta gtatgcctga aatcttcttt aggatttacc cactgcaata aaacatttac
     5821 tccggtttta aatgctccaa cttgagtcgg aggagcgggc ggaataatat ccgccgcaga
     5881 gaccaaagca aaccgagtta aatgcgaaaa actcgctaca actctggtgt ttaatttatc
     5941 cacatagtaa tcagctactc taacccataa tccagactca tcatcataaa aacttggcac
     6001 caattgatct gccgttattc ccaaaccagc caatagttta acggtataag ggaatgataa
     6061 agtaatcttt ttattaaagt tagtaattgc aactccatct ttatctttca agtcaatatc
     6121 ataaacggct ccgactatgt tattaaactg caccggaact tcaactgttg gacttatact
     6181 aactgttaga tctcccgcaa gcgaaacaag atctgccgga atctcgattt tagcccgatc
     6241 agtaagattt ataaccgaac ccgattgaat attagttgtt tgcgacacct gatcgggaag
     6301 cgttatatcc tcggcattca aaactaaatt gcccaatgtc actgatgcgc ccctaatatc
     6361 aactactatc tctgctgacc tatatggagt ttttccttcc agtttggatg ctcctacgcg
     6421 ccacctcaag tcagtagaaa cgggcagact aaattgtccg gctgaagttg ctcttgtttg
     6481 aaccgttcta ccatcattag accaccccca tatcaaagca tctgtcggcc tgccgtcggc
     6541 taatcttgcg gttccggtga taaacgcttt caccgaaccg aattgtatat aggttgccgt
     6601 ttctgtattg ctaaatactt gcgcggattt ttcttgcggc tgcgtgaagc cgacttcaga
     6661 cgatcttgct ctgatataaa aagtgcctgg cgataaatta aaactgacca atccatcctg
     6721 atcactcacg ccaacaactg ttgtgccgga atagagacct aacgccctgt cttttgcgct
     6781 tgtgctatca attataaccc ccgctccgac aagcgccctg ccatctggcc ccaaaacttt
     6841 tacagatagt ttcgcgttcg ctagaggaac tgtaaagtca accgtccgtt gctcggaaag
     6901 agaattatcc gcaaaagaca cagtttgtat aggttcgctc catgtccaat tagacgacgc
     6961 aagcggcgca atatttacag tccattgacc tccgcttacg gtaaaggaat atgaaccatt
     7021 aaaagatgac aacgcggtca tataagatcc ggttgaagaa ttatatattt ttacctctgc
     7081 atccgtaacc gcagaattgt tagaaaatat tactttcccg ctaatcgttt ttgcgtctcc
     7141 ggtctggcca ggaggaggcg gaggaggcgt cggttgaggc gaagaattag ttgttgcctc
     7201 cgccgcgtcg ctaaaacctg aaagcgcgcc gaatgaatcc ttggctctta ctttatatgt
     7261 ataagatgtt gaaggagaaa gtcccgtatc tgtatagctt gtgccgttaa cggaagttaa
     7321 ttcggatgtg ccgcgataga tcgtataact tatagtgtta ttttccggat ccgttgatgc
     7381 attccaagtg atttttatgc ttgtagttga ttctgggctc gctgaaacat tttgcggaac
     7441 agatgggggt tgattctctc ctgatccaag cgtaactaca gtaatagtgt tactaaatga
     7501 agataaattg ccggcatcgt ctttggccct tactttataa gtataggttg tattagcgct
     7561 taatccgcta tttgaaaaac ttaacgcgct caccgtctca attaattgaa aattcaatcc
     7621 taacgcgcta ccacgatgaa tctcatatcc cgttacgcca acattgtctg ttgatccact
     7681 ccatgaaagt tgaacgctat ttgaggaagg tgtgccagac aaattggtcg gagcagttgg
     7741 ggcagtcgta tccggctgac cttgaccacc aatggtatta ctaattgtaa tcgttctgcc
     7801 cgctgatgct gtttgattcc gcggcgtggc tgaatcataa gctgtcgctt ttaaagtata
     7861 gctaccattc ggatataagg cagaattcca atttatatga tatgtatcac cgccgcttgg
     7921 cgtaggattc gaacctatat cgtaagaatt ggttgaatta cttatgttga aaacaacttc
     7981 agacacccca cttcctgaat cagtcgcagt agcagctgct tccgctgtac cagacacagt
     8041 ggcattatct ccaggtaaag ttagattaac gttggtggga ggagtgttat ctttagtagt
     8101 aaattcatca tcatattcac cagtacctga ttggttaatt ccatatatac caaatgattt
     8161 tgctttaaaa ttataacgct tcccttcttg taagccagtt attcttatac agtgctctgt
     8221 agtcggtgtt gccgcattag aatacccagg acaactatta cttacttgaa tattaggaga
     8281 gttaggatgt atagcataat caacttttga atcagataaa tgattatctg tacgccaaaa
     8341 aatatcagct gtagtagtcg tagtagaagt actaacattt gttatttctg gtggtgcgct
     8401 ggctaaagag attggaacat atagccagaa taaagatgct attataaata atatcaaaaa
     8461 tttattcgta taaatcatta gaaataatta tacaacattc aaataaatca aaacactagc
     8521 gcctgtttat aagttaggaa ttggcacaac cgaagaagaa cataatgtat cgccaaataa
     8581 attacacaca ttcaatctcc ctccactcat gctggttcct gtcatagaaa ctatcttatc
     8641 cgttctctga agcaacctat cttttatagt taaatgatct atatcttgat tgtatgacca
     8701 atataaagct atcgctccag atgtaaat
//
LOCUS       DATDNZ010000035         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3256977, whole genome shotgun sequence.
ACCESSION   DATDNZ010000035 DATDNZ010000000
VERSION     DATDNZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256977"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VI432_01350"
     CDS             <1..1143
                     /locus_tag="VI432_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4496781.1"
                     /translation="YHTYTPTLYEAATTSKDVADDIKIQQITAFPIENILKRTSIEFI
                     QNKVINLDIGIRGGTIDLDNGFNIHYEYLILALGSEANFFNIPGLQEYAIKLKEFDDA
                     IRIRDLIWNKATSKPPEDKDKKVKVVIGGAGSSGVEVAGEIKNWLKQLEKEHEIEGFV
                     TIVEAGETILPGFAPQIINLATRRLKKLAIEISTSSTIEKINQNTVYLKNAGEIPFDV
                     LIWTGGVKANTLASTIPIKTVKTRAEVTSKMVCLPQSADLKFYGKVYAIGDLVCAYNK
                     DGKPSPMVARAAIIQAKIASRNIICDIMNKRHREYSFKNYPYVIPIGGKYAIAKLGPI
                     IISGFFGWILKGLVELNYLLSIMPTWRALSVWLKGLKIFIQNDRLG"
     gene            1152..2291
                     /locus_tag="VI432_01355"
     CDS             1152..2291
                     /locus_tag="VI432_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY4496782.1"
                     /translation="MKNKKHSYGIQALKLHKKLAGKIEITSKIKIKTKEDLSTVYTPG
                     VGSVSLYLAKNKNKTGEYTIKGNSVAIISDGSAVLGLGNIGPEGALPVMEGKAMIMKE
                     FAGLDAIPIVLNTQNTEEIISAVKAIAPTFGAINLEDISAPRCFEIEERLKQELDIPI
                     MHDDQWGTSVVVLAALINATKLAGKNIKKSRIVILGAGAAGSAIIKILHKYGARNIIA
                     VDRDGIIHVNDNSPAKAKLAEITNPQNEKGNIEDALIDADVFIGVSKGGLVTEEMIKK
                     MADKPIVFALANPVPEIMPDAAKRAGAYIVASGRSDFPNQVNNALAFPGIFRGAIDNK
                     VKKITKTMLIRAAENIAKSIKPSKEKIIPNIFDKRIIKIVSRAIK"
     gene            complement(2315..2809)
                     /locus_tag="VI432_01360"
     CDS             complement(2315..2809)
                     /locus_tag="VI432_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496783.1"
                     /translation="MNKSAIFIIILIVIGAVILIYQDQIFSFFSPGAVIVNDDESIIV
                     PSQNTISQEIEDFDDGAIIPTVPLSRQANQIIDITTIGFSPSETTIDKGDSIIWTNRD
                     RNSHKLQATGAIYPEDGDCGSKLNSCAALKLGDSWKVTFNVSGEWNYRDELNPQFTGV
                     VTVR"
     gene            complement(2814..>3135)
                     /locus_tag="VI432_01365"
     CDS             complement(2814..>3135)
                     /locus_tag="VI432_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY4496784.1"
                     /translation="DASLRSSATSDAERIVYEYKIEADDSGLYPASIEVPRGERVKIN
                     FIVRSENVYYGGLDFRSSKFKTERVSPGGTISVEFVADESFEFSSYWPASDVLKKTGK
                     VVIK"
BASE COUNT         1091 a          641 c          592 g          811 t
ORIGIN      
        1 tatcacacct acacgccgac cctatatgaa gccgcaacca catctaaaga cgttgctgac
       61 gatataaaaa ttcaacagat aaccgctttt ccgatagaaa atattctaaa acgaacatca
      121 attgaattta tccaaaacaa agttatcaat cttgacatag gcatacgcgg cgggacaatt
      181 gatttagaca acggctttaa tattcattat gaatatttga ttctcgccct tggatccgaa
      241 gctaatttct tcaatattcc aggactgcaa gagtatgcca taaaactgaa agagtttgat
      301 gacgccataa gaataaggga tttgatttgg aataaggcca cctcaaaacc accggaagat
      361 aaagataaaa aagtaaaagt tgtaattggt ggagccggat caagtggggt tgaagtcgct
      421 ggagaaatca aaaattggct taaacagctt gaaaaagaac acgaaattga aggcttcgta
      481 accatagttg aggccggaga aacaattttg ccgggctttg cgcctcaaat aataaaccta
      541 gcgacgagaa ggcttaaaaa acttgcaatt gaaatatcaa cctccagcac tatagaaaaa
      601 ataaatcaaa atacagtata tttaaaaaac gcgggagaaa ttccttttga tgtcttaatt
      661 tggaccggcg gagtaaaagc aaacacgctc gcctcaacca ttccaatcaa aaccgtaaaa
      721 acaagagccg aggtaacatc aaaaatggtt tgcttgcctc aatccgctga tttgaaattc
      781 tatggcaaag tttatgcaat cggagatctg gtatgcgcct ataataaaga tggaaagccc
      841 agtcccatgg tcgcgcgagc tgcaattata caggctaaga tcgcgtcaag aaatatcatc
      901 tgcgacataa tgaacaaaag acacagagaa tactctttca aaaattaccc atatgtaatc
      961 ccaatcggcg gaaaatacgc gattgccaaa cttggtccga tcataatttc cggatttttt
     1021 ggctggatat taaaagggtt ggttgaactt aattatttat tatcaataat gccgacgtgg
     1081 cgggctttgt cagtctggct aaagggttta aaaatattca tacaaaacga tcgtttaggt
     1141 taaaaatcaa aatgaagaat aaaaaacact catacggcat tcaagctcta aaactccata
     1201 aaaaacttgc cggaaaaata gaaattacat ctaaaataaa aattaaaaca aaggaagact
     1261 taagcactgt ttatacgccg ggcgtaggaa gcgtttcatt atatcttgct aaaaacaaaa
     1321 ataagaccgg agaatatacg attaagggta atagcgtggc cattataagc gatggctctg
     1381 cagtcttggg cttgggaaat atcggcccag aaggagcttt gcctgtcatg gagggcaaag
     1441 caatgattat gaaagaattt gccggcttag atgcgatacc gattgttttg aatacgcaga
     1501 acaccgagga aattataagt gccgttaaag ccatagctcc aacctttgga gccattaatc
     1561 ttgaagatat ttccgctcca cgctgttttg aaatagaaga aagacttaaa caagaattag
     1621 atattccaat tatgcatgat gaccaatggg gaacatctgt cgtggtttta gctgcgctta
     1681 taaacgcaac gaaactcgct ggtaaaaata taaagaaatc tagaattgtc atcttagggg
     1741 ctggtgctgc cggaagcgct attataaaaa ttttacataa atatggagcg agaaatataa
     1801 tcgccgtaga ccgcgacggc atcatacatg taaatgataa ttctccggcc aaagccaagc
     1861 ttgccgagat aacaaacccg caaaacgaaa aagggaatat agaagacgcc ctaatagatg
     1921 cggatgtttt tatcggagta tcaaaaggcg gactggtaac tgaagaaatg attaaaaaaa
     1981 tggccgataa accgatcgtc tttgctcttg caaatcccgt tccggaaatt atgcccgatg
     2041 ccgccaaacg cgcaggcgct tacattgtcg caagcgggag atctgatttt ccaaatcaag
     2101 tcaataatgc gctcgcgttt cccggcatat tccgcggagc aatagacaac aaagttaaaa
     2161 aaatcactaa gacaatgcta atccgcgcgg cggagaatat tgcaaaaagc ataaaaccct
     2221 caaaagaaaa aataataccg aatatcttcg acaaaagaat tattaaaata gtgtctagag
     2281 caataaagta aaatagatca aaccgtctct ctgcttatct taccgtcacc acgccagtga
     2341 attgaggatt aagctcgtcg cggtaattcc actcgccgga gacattaaaa gtaaccttcc
     2401 agctatcgcc cagctttaat gcggcgcagg aattaagttt actgccacaa tcaccatcct
     2461 ccggataaat tgcacctgtc gcctgaagtt tatgagaatt cctgtctctg tttgtccaaa
     2521 taatagaatc tcctttgtct attgtcgtct cacttggact aaaaccaatc gtagtaatgt
     2581 caattatttg attcgcctgc ctactcaatg gaacggtcgg gataatagcg ccgtcatcaa
     2641 aatcttcaat ctcttgactg attgtgtttt gagatgggac tattattgat tcatcgtcat
     2701 taacaataac tgcacctggc gaaaaaaaac taaatatttg atcttgataa attaatataa
     2761 ctgcacctat tacaattaaa ataataataa aaatagctga tttattcata tcattatttt
     2821 atcacaacct taccggtttt tttaagaaca tcagaagccg gccagtaaga cgaaaattca
     2881 aaagactcat cagcaacgaa ctcaaccgat atcgttccac caggagacac cctctctgtc
     2941 ttgaactttg agcttctaaa atccaatccg ccataatata cgttctcaga cctaactata
     3001 aaattaatct taaccctttc tcctctggga acttcaattg atgccggata aagcccgcta
     3061 tcatccgcct caattttata ttcatagacg atcctctctg catctgaggt tgcgcttgat
     3121 ctcaaactcg catca
//
LOCUS       DATDNZ010000036         3022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1678606, whole genome shotgun sequence.
ACCESSION   DATDNZ010000036 DATDNZ010000000
VERSION     DATDNZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678606"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VI432_01370"
     CDS             complement(<1..507)
                     /locus_tag="VI432_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY4496785.1"
                     /translation="MSKILGIDLGTTYSAVAIVEGGEPKIIENSEGARTTPSVVALSK
                     NNERTAGLLAKRQAVTNPKNTIFSVKRLIGRKFSDPEVARDKAWLPYDIRESSDGGVE
                     IKMGDSSTSSVQGKWYKPEEISAMMLSKLKADAETRLGEPINDAVITVPAYFDDSQRQ
                     ATKNAGEIA"
     gene            complement(520..1017)
                     /locus_tag="VI432_01375"
     CDS             complement(520..1017)
                     /locus_tag="VI432_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY4496786.1"
                     /translation="MAEEFSPSTSSGQENNQSESEIERLKVEREEYLNGWKRAKADLI
                     NYKKEEEETIGKAIKYGNEGIIRETINILDSFDLAISSIKDVEIAKGFEVIKNQLEGA
                     MAKYGLRNIEIKQGDKFDPQYHEAIGMVDQKDNLVSGDIAEEVSRGYMIYDKVVRATK
                     VLVIK"
     gene            1220..2044
                     /locus_tag="VI432_01380"
     CDS             1220..2044
                     /locus_tag="VI432_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496787.1"
                     /translation="MQMGEEGKNRIRQHLADAHAFTFDGNTINALQKTKADTMDALPF
                     PICFLDAELDASIKGMTVKVRGILIGDFGVQLLEHRPGLIGSMRKLLPKKMRTSKGPS
                     CFTTAIVEPNDPSRKPFRFWNMNPLVGSFNARVANGGHASDTENEKLVRKVETEVKNF
                     VAAWQNLLFDPEVKMVEVRNSPNARKRIEREHGYFPPIIHQIRISGKLKTYLQKQARL
                     VKGHGDMRMHWVRGHFRHLQSKRFTHKHGQRIWVPPHIRGASGPLIRSEYAVTQKK"
     gene            complement(2118..2723)
                     /locus_tag="VI432_01385"
     CDS             complement(2118..2723)
                     /locus_tag="VI432_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY4496788.1"
                     /translation="MKIPSSINNFVEKIAGLPGIGPRQALRIAFHLVYKGEGAKKELE
                     KSVKGLESIKICSRCFYLYEGDGKICDICSDKKRNHAIIAILEKPTDLISIENTSKFE
                     GRYLILGDLRKKGVLDVDQKLKLEGLKKWINEFLPEGLAEEIIIAVNPTSIGDLNASS
                     IYDELKKYSRKITRLGRGIPSGGEIEFADEDTLGSALDRRI"
     gene            complement(2701..>3022)
                     /locus_tag="VI432_01390"
     CDS             complement(2701..>3022)
                     /locus_tag="VI432_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4496789.1"
                     /translation="LARELDVPVIAISQLSRAVDQRETKMPRLSDLRESGSIEQDADV
                     VLFVHPKDKGLGQDALEENKNLVEIIIAKHRNGPLGTVKLYFNKEKVTFQSIDMTHED
                     PIIN"
BASE COUNT          810 a          711 c          513 g          988 t
ORIGIN      
        1 tgctatctca ccggcatttt tagtggcttg tctttgcgaa tcatcaaaat acgcagggac
       61 tgtaatcacc gcatcattta tcggctcgcc aagccgagtc tctgcatcag ctttcaattt
      121 agaaagcatc attgctgata tttcttctgg tttataccat ttaccctgca ccgagcttgt
      181 tgaagaatcg cccattttaa tttcaacgcc accatcagaa gattccctaa tatcataggg
      241 aagccaagct ttatctcttg caacttctgg gtcagaaaat tttcttccga tcaacctttt
      301 aactgaaaaa atcgtgtttt tgggatttgt aaccgcctgc cttttggcta aaaggccggc
      361 tgtcctttcg ttattttttg acagcgccac tacagaggga gtagttcttg ctccctctga
      421 attttcaatt atcttaggct cacctccttc aactattgct accgctgaat atgttgttcc
      481 taagtctatt cctaatattt tgctcataat ttttattttt tattttatta ctaatacttt
      541 tgttgctcta acgactttat cataaatcat ataccctcgg ctaacttcct ctgctatgtc
      601 tccagacaca agattatcct tttgatcaac catgcctatc gcttcatgat attgaggatc
      661 gaatttatct ccctgtttta tctcaatatt tcttaacccg tacttagcca tagctccttc
      721 cagctggttt tttatcacct caaaaccctt agcaatctct acgtctttta ttgagcttat
      781 agccagatca aagctatcta atatattgat tgtttcgcga ataataccct cgttgccgta
      841 ttttatggct tttccaatcg tttcttcttc ctctttcttg taattgatca aatcagcctt
      901 agctcttttc catccattca aatattcttc acgctcaacc ttgagtcttt cgatttcgga
      961 ttcagattga ttattttctt gccctgagct tgtcgaaggg ctaaattctt ctgccatcag
     1021 agctacagca taaaaaaatg atatcggtat gtcaacttta gaatatattc tcgcatttca
     1081 tataatcctt tagacgcttt aaatttgacc aaatttagat tttatgacaa agttgcctta
     1141 tgatccttaa aaacctgacc atggaggaga acgcttgagg ttctacggcg aacatttctc
     1201 agagctcact gacccatcta tgcaaatggg tgaggaaggc aaaaaccgta ttcgtcagca
     1261 ccttgcggat gcacatgcct tcacttttga tggcaacaca atcaacgctc tccaaaaaac
     1321 gaaggctgat accatggatg ctttgccatt tccaatatgt tttctagacg ccgaacttga
     1381 tgcaagcatc aaagggatga ctgtaaaagt ccgcggcata ctaattggcg acttcggagt
     1441 acagctctta gaacatagac ctggattgat aggatctatg agaaaacttc tgcccaaaaa
     1501 gatgagaacg tctaagggtc caagttgttt tacgactgca atagtcgaac caaatgaccc
     1561 aagcaggaag cctttccggt tttggaatat gaatcctcta gtagggtcat ttaacgcccg
     1621 ggtggctaat ggcggtcatg cctccgatac tgagaatgaa aaactcgttc gaaaagtcga
     1681 gaccgaggtt aaaaacttcg ttgcggcttg gcaaaacttg ctctttgacc cggaggttaa
     1741 aatggttgag gtgcgaaatt caccaaacgc gcgcaagaga attgagcgcg aacacggtta
     1801 cttcccgcca ataatccatc aaattcggat tagtggtaaa ctcaagacgt atttacaaaa
     1861 acaagccagg cttgttaagg ggcatggcga tatgcgtatg cactgggtgc gtggacactt
     1921 tagacactta caatccaaaa ggtttacgca taaacacggc cagcgcattt gggtacctcc
     1981 ccatatccgt ggcgcatccg gacccctcat taggtctgag tatgcggtaa cccagaaaaa
     2041 atgagactgc ctttgtcacc gaactgatga cttttctccg ttttaaaaat cccgaatcaa
     2101 ctgattcggg ttctttttta tatcctccta tctaaagcgc ttcctaaagt atcctcatcc
     2161 gcaaactcta tttctccacc gcttggtatt cccctaccta acctagttat ttttctggaa
     2221 tattttttta gctcatcata aatagaagac gcatttagat ctccaataga tgtgggattg
     2281 accgctatga ttatttcttc cgccaagcct tcgggcaaaa attcattaat ccattttttt
     2341 aatccttcca attttaattt ttgatcaaca tcaagcactc ctttttttct caaatctcct
     2401 aaaatcaaat atcttccctc aaacttgctc gtattctcta tggatatcaa atcagtaggt
     2461 ttttctaaaa tcgcaatgat agcatgattc ctttttttat ctgaacaaat atcgcaaatt
     2521 ttgccgtctc cttcataaag ataaaaacat ctggagcata tttttatact ctctaatcct
     2581 tttaccgact tttccaattc tttctttgct ccttcccctt tatacacaag atggaaagct
     2641 attctaagcg cctgcctcgg acctatgcca ggcagacccg caatcttttc tacaaaatta
     2701 ttaattgatg atgggatctt catgtgtcat gtcaatactt tggaatgtga ctttttcttt
     2761 attgaaataa agcttcacgg ttccaagagg accattccta tgtttcgcga taataatctc
     2821 aactagattt ttgttttctt caagcgcatc ttgtcccagc cccttgtctt ttggatgcac
     2881 gaataaaacc acatctgcat cttgctcaat gcttccgctc tctcttaggt ctgataacct
     2941 aggcattttt gtttcacgtt gatcaaccgc acgagacagc tgtgaaatcg ctataaccgg
     3001 cacatctaat tctctagcca gg
//
LOCUS       DATDNZ010000037         3891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1162804, whole genome shotgun sequence.
ACCESSION   DATDNZ010000037 DATDNZ010000000
VERSION     DATDNZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162804"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..961
                     /locus_tag="VI432_01395"
     CDS             464..961
                     /locus_tag="VI432_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496790.1"
                     /translation="MPDAKFKILNSEADQRGVVVLAMVIIIVSITVLIGISLARTSFF
                     ELASGSSEAKTEEALRAADAGIQDALIQLVRNPDFLCTGSGANCTLTATCTVDSGTDG
                     YALSVGNGTACVKITNPSAPCPETGLTIKSRGTISNKIRKIQVTFDFDSNCGITQTAW
                     TELTS"
     gene            1120..2760
                     /gene="pilM"
                     /locus_tag="VI432_01400"
     CDS             1120..2760
                     /gene="pilM"
                     /locus_tag="VI432_01400"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY4496791.1"
                     /translation="MKLPKPKLKKVKETPAIAKPSESAKPPSVHPKNGIKLRFPNLVS
                     FFSGQHAVAGIAISENNIYSVVLNNFRGAQPTVVSSQQVALPQGTFVEGKLNKPQDLV
                     AALIKLRSKAKTPFCIFSLPGSEWWMRLFFFPASLTNVQFEDSMQFHLSLDLPWDPKE
                     AYADWEEIDADEADKRSALLVAAKISAITPYLDVLERAGWTTLAIEPASLSIIRAIAH
                     FEADVPAIVLGYDGTILEEMIVWKGKMHFGRVVSLPAQESVFKSDEKYNLSTKPAVSV
                     VEPLGTSPAKGLDVYMAVELKRLMAYVQTEPLGMPKPTHLFFVGPFSQTHINKLAEVA
                     RNYGLTEKVFAASSLPWGVALRGLIPRDKDNLISLMPVGTEESYTRKRAALFFEFALN
                     FSFLVVLILAVSFAGAWFFVRNEKQKSIERLARIKGNTTQIQATIEGLQEFTDASALA
                     ISVAQATPHWDGIYGKILDAQVLGITIYKITLNVSGAVKIQGKTEKPSNLLAFRDALQ
                     NKNLGQNIVIPANLFESGGTGDFVLVFNLPDTKEPFNP"
     gene            2785..3333
                     /gene="pilO"
                     /locus_tag="VI432_01405"
     CDS             2785..3333
                     /gene="pilO"
                     /locus_tag="VI432_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY4496792.1"
                     /translation="MNRETISRILKNFSPFFYVRLIMLFVFIAVILFMKGKINSISQD
                     VNRTLNSIAAQTANLNNSARLKQQYDALHPFVPKIKNLIPDSDKLPIFVADIEAVSEK
                     VGVKSSVRFGLEPVPSSAVTNTKEIQIFLNSVGSGKAFVAFLKELATMPYFVRLNDVE
                     IDAAVGLDSTSTLKANGTLFLK"
BASE COUNT         1174 a          777 c          771 g         1169 t
ORIGIN      
        1 tattacacac cacccccaag cgctggaatt aaggtaacct gttatgcgca gcacaacaac
       61 aaacgctact tccgctataa agtattcctt gatgctaatg tcacgcgtga tcttataccg
      121 aaagtggatg acatcattgt cgcttggtcg ccttaatacc taaattctaa atccaaaata
      181 ctagattcta aactataaaa ctgcccctca aaaaggctta aaaatagtaa ttattttgcc
      241 taataaccaa cgcctcgtta ttaaagcgtt atccaagtat ttgatacgct tttgccgcaa
      301 aataagctat aatactattg gaggctcaca gaagtatttc agtaaagaaa taaggacaac
      361 agtagaattg cgatagaatt tagaatctgg aatatagaat ttggaataat gtgtttaact
      421 cgttttaaat tctgacttct aactcctaaa ttccaaaagc aaaatgccag atgccaaatt
      481 caaaattcta aactctgaag cagaccaacg cggtgtggtt gtacttgcta tggttataat
      541 aattgtatcc attacggttt tgataggcat atctttggct agaaccagct tttttgagct
      601 tgcaagcgga tcaagtgaag ctaagaccga agaagccctc cgtgcggccg atgccggcat
      661 tcaagatgcc ctgattcaac tggtaaggaa cccagacttt ttgtgtacag ggtcgggtgc
      721 taattgcacg ctaactgcaa cttgtacagt ggattccggc acagatggat acgcgttatc
      781 tgtggggaac ggaaccgcat gcgttaaaat taccaatcct tcagcgccat gtccggaaac
      841 gggtctaacc ataaaatctc ggggcactat tagtaataaa atacgtaaga tccaagttac
      901 gtttgatttt gatagtaatt gcggcataac ccaaaccgca tggacagaat tgactagcta
      961 aaaaatttct aattactcta atttctaatt gatctaatca agtcccaaat gactaatttc
     1021 tatgtttaga catttaagca ttaggtatca gttcgcctcc atcagttggc tgacggcgaa
     1081 tggattagaa attaggaatt agatattagg cattcgcaaa tgaagcttcc taaaccaaaa
     1141 ttgaaaaaag taaaggagac tcctgctatt gctaaacctt ccgagagcgc taaaccgcct
     1201 tcggtccatc ccaagaacgg tataaaacta cgttttccaa atctcgtttc ctttttttct
     1261 ggccaacacg cggttgccgg tatcgccata tccgaaaata atatttactc cgtggttttg
     1321 aataatttta gaggcgcaca accaacagtt gtttcaagtc agcaagtagc cttaccacaa
     1381 ggaacttttg ttgaaggtaa attgaataaa ccgcaagatc ttgttgctgc tcttatcaag
     1441 cttagatcaa aagccaaaac accgttttgc attttttctt taccgggaag cgaatggtgg
     1501 atgcgactgt tttttttccc tgcctcacta accaacgttc aatttgaaga ttcaatgcaa
     1561 ttccatcttt ctttagattt gccatgggat cccaaagaag cctatgcgga ttgggaagag
     1621 attgatgccg atgaggcgga taagcgatcg gccttactag ttgcggcgaa aatttcggct
     1681 atcacgccgt atcttgatgt tcttgaaaga gcgggttgga cgacattggc aattgagccg
     1741 gcctctttaa gtattattag ggctatagcc cattttgaag ctgatgtacc agccattgta
     1801 ttgggttatg acgggactat tcttgaggaa atgattgtat ggaagggcaa aatgcatttt
     1861 ggtcgtgtgg tatccttacc cgcccaagaa tcagttttca agtcagatga aaaatataac
     1921 ctctcgacca agcctgcggt gagcgtagtc gaaccactag ggacaagtcc tgccaaaggc
     1981 cttgatgtgt atatggcagt tgaactcaaa cgtttaatgg catatgtcca gacagagcca
     2041 ctgggaatgc ccaagcccac gcatttattt tttgtaggac ctttcagtca aacgcacata
     2101 aacaaattag cagaagttgc aagaaactac ggccttaccg agaaagtttt cgcggcatca
     2161 tctttaccgt ggggcgttgc cttgcgtggt cttatcccgc gcgataaaga taatctaatt
     2221 tctttaatgc ctgtaggtac cgaggaatct tatacccgca agcgtgcggc attattcttt
     2281 gaattcgctt tgaatttttc ttttttagtt gttttgatct tggccgtttc atttgctggc
     2341 gcgtggtttt ttgtccgcaa tgaaaaacag aaaagcattg aacgtttggc gcgcatcaag
     2401 ggcaatacaa ctcaaataca agccactatt gaagggcttc aagagtttac tgacgcgtct
     2461 gcattagcga tatctgttgc gcaagcaact ccgcattggg acggtatata cggtaaaatt
     2521 ttagatgctc aagtcctggg cataactata tataaaataa cccttaatgt ttcgggagct
     2581 gtaaaaattc agggtaaaac cgaaaaacca tcaaacctac tagcgtttag agacgcactt
     2641 caaaataaaa atttaggaca aaatatagtg attccggcaa atttatttga aagcggcgga
     2701 acgggagatt tcgttttagt ctttaatctc ccggatacga aagagccttt taatccatga
     2761 taagcaaaga ccagttagat tattatgaat agagaaacta tatccagaat attaaaaaat
     2821 ttcagcccgt ttttttacgt gcgtttaatc atgctgtttg tttttattgc ggtgatttta
     2881 tttatgaaag ggaagatcaa tagcatctct caagacgtta ataggacttt gaacagcata
     2941 gctgcacaga cagcaaattt aaataattct gccaggctta aacagcaata tgacgcatta
     3001 catccgtttg tgccaaaaat taagaatctt attccggatt cagacaagtt gcctattttt
     3061 gttgctgata tagaggcagt ttcagaaaaa gtcggcgtga aaagctctgt gcgtttcgga
     3121 cttgaacccg ttccttcgtc ggctgttact aatactaaag aaatccagat atttttgaac
     3181 agcgtcggca gtggaaaggc ctttgtggct tttttgaaag agcttgccac tatgccttat
     3241 tttgtaagat taaatgatgt tgaaatagat gccgcagtag gtctggacag cacctcaacg
     3301 ctcaaggcta acggaactct tttcttaaaa taaatgtttt taatcaaaaa ttgaaccccg
     3361 ttagaaaatt gaatttttat gaaaatatat gttctaatac aacttaattg gctatgtggc
     3421 ttccgccaaa aaagtcagca gactttttcg gccgcaagcg catgtccaat tttttaatgg
     3481 ggtatgctaa aaaagggctt gaaattagac cgtaaaacgc ttaggaatta tatttatccg
     3541 ataatgctcg ttttggttat ttttgtttcg cttattatat tttatttctc gttgcgcttt
     3601 ttgattaaat caatatccac tgctttattt atgggtagtg ccgaaacgcc agcaccatct
     3661 ttccaaaacg aaaatctcaa gtatcttaag cggtatatac cagatctgcc tctatctctt
     3721 taatttttta agtaatccac tattaacaag aaggcgacaa atttcgtata agtcgcattt
     3781 ctactacctg atatgccact acaaaacacc gcagctaata gatggttaga agtattatcg
     3841 gattcttttt tcgttatcgc ccaatcttta actcaagcct taccagcatt t
//
LOCUS       DATDNZ010000038         2744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_617658, whole genome shotgun sequence.
ACCESSION   DATDNZ010000038 DATDNZ010000000
VERSION     DATDNZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617658"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VI432_01410"
     CDS             <1..424
                     /locus_tag="VI432_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY4496793.1"
                     /translation="KEFLPDGKEILEHCKDKGYKLALAVIASEFEERKKQIGESGLAG
                     FFEIIRIAPITKEQIWDHGINVKDRLYDEIMNEWDFKPEEVLIVDDRMFRGIKHGNKN
                     GHPTVWVRNGKFAHEIPNKETGEPTYIINSVKELRDLI"
     gene            complement(554..916)
                     /locus_tag="VI432_01415"
     CDS             complement(554..916)
                     /locus_tag="VI432_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496794.1"
                     /translation="MSEITPIIKVWCLDITDETELHSLHNAIVDAVIAIPELGVKSES
                     DMISLYPPDLMRYGLGEAVIVEAFLFCKKQRPTDDVIDRLAKDLAQVIMKHCPKVQKT
                     ECFVNDTNSKRGVWIEKR"
     gene            1167..1622
                     /locus_tag="VI432_01420"
     CDS             1167..1622
                     /locus_tag="VI432_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496795.1"
                     /translation="MMKKMSKNIKTVIEILQNEVDGNVAFALKKVTEDYTMTWMERTK
                     KGDLFPTETMDTGQDIDEIYAIKGRQYDIKNIAEGDSVVMVELVESYPDPKTKKVYRT
                     PLVLVLEMKNGKIRTGRHYMGPKLSGEFLTKAQINKVYKKSKGSMKIIK"
     gene            1642..2157
                     /locus_tag="VI432_01425"
     CDS             1642..2157
                     /locus_tag="VI432_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496796.1"
                     /translation="MNINLKNNSEKEQKTKKLLEGLFNRFNLDKWILCDEILIEEGGR
                     GKAFPFIILSAGLNEDILLAQFLHEQSHWVHGNIVGAENMDKAIDELKEIFKSVPIDK
                     PEGGGSEKSTYIHLIICRLEFLALKELLGEEKAMNIVSNNNNYTWIRKTILDSSPIID
                     QIIERYFPRIY"
     gene            2202..2687
                     /locus_tag="VI432_01430"
     CDS             2202..2687
                     /locus_tag="VI432_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496797.1"
                     /translation="MAKNNTKNLIYIVGGLLIIGGLFAWPIITDETRASVKDWNKAGV
                     GCLLGHQQVVSHIHQQLLITVDGEEETIPPNTGIARGCMAEIHVHEGEANILHVETAV
                     AGKEMKLGHFFAVYDKPINRDGYDLIMGVNGEAYEGDPLEFVLQDQQVIEIEYNSETA
                     Q"
BASE COUNT          928 a          390 c          644 g          782 t
ORIGIN      
        1 taaagaattc cttccagacg gtaaagaaat attggaacat tgtaaagata aaggatataa
       61 attagctctt gccgtaatcg cgagtgagtt tgaagagagg aagaagcaga taggggaatc
      121 tggtttggct ggctttttcg aaattatccg catagcgccg attactaaag aacagatttg
      181 ggaccacggc attaatgtaa aagataggct ttatgacgaa atcatgaatg agtgggattt
      241 taaacctgaa gaagttttaa tagtagacga tagaatgttt cgcggtatta agcatgggaa
      301 taaaaatgga catcctacgg tatgggtgcg gaacggtaag tttgcccatg agattcctaa
      361 taaagagaca ggcgagccaa cttatataat taattcagtt aaggagttaa gagatctgat
      421 atagaaaaca cgttgataca gataagagag aaataaaaag agacgaccta agtggtcgtc
      481 ttttgtttca tccccctttt gtccccatgg ggataaaagg gggatgagtt ttgcgatgga
      541 tggaaaagtc tacttagcgt ttttctatcc aaacgccgcg tttcgaattc gtatcgttca
      601 cgaagcattc agttttttgt actttaggac aatgcttcat gatgacttgc gcgagatctt
      661 tggcgaggcg atcgattaca tcgtcggtag gtctttgttt tttacagaat aaaaatgctt
      721 cgacgataac cgcttctcct aatccatatc tcatgaggtc tgggggataa agactgatca
      781 tgtcgctttc gcttttcacc cctagttctg ggattgcgat cacagcatcg acaatcgcat
      841 tatgaaggct gtgtaattct gtttcatcgg tgatgtctag gcaccatact ttgatgatcg
      901 gcgtgatttc tgacaatttg agaactcctt gttatagagc tgtttttaga atgacattgt
      961 ttagagtaat tgtcaattta ggattatatc tatttcaagt ataatgttct aatattgcgt
     1021 ttatcataaa accaatatta aggcgagcac tttgttatac caataaccac gctatcgctg
     1081 gatgattggt cttggttgaa gtttgttaag tcgaactata aaaaatgaga aagaagagtt
     1141 tgatggggct gtgataaaat agatttatga tgaaaaagat gagtaagaac attaagactg
     1201 ttattgagat ccttcaaaat gaagttgatg gtaatgtggc ttttgcgctc aaaaaagtta
     1261 cagaagatta tacaatgact tggatggaga gaacgaagaa aggagattta ttcccgaccg
     1321 aaacaatgga tactggtcaa gatattgatg aaatctatgc cataaaagga cgccagtatg
     1381 atattaaaaa tatcgcagag ggcgatagcg tggtgatggt tgaactggtt gaaagttatc
     1441 cggatcctaa aaccaaaaag gtatatagaa cacctcttgt acttgttctt gagatgaaga
     1501 atgggaagat aagaacaggc aggcactata tgggccctaa attatcagga gaatttttga
     1561 ctaaagctca aattaataag gtgtataaga aaagcaaggg ttctatgaag attattaaat
     1621 aggtgattta tgttgagata aatgaatata aatcttaaaa ataatagcga gaaagagcag
     1681 aaaaccaaga aattattgga aggattattt aatagattta atttggataa atggattttg
     1741 tgtgatgaga ttcttattga ggagggagga agaggcaaag cgtttccatt tattatatta
     1801 agcgctggac taaatgaaga tatattactt gcgcaatttt tgcatgaaca atctcattgg
     1861 gtgcacggaa atatagttgg cgcagaaaat atggataagg cgatcgatga attaaaagaa
     1921 atttttaaaa gcgttccgat agataagcca gagggaggag gtagtgaaaa atctacgtac
     1981 attcatttaa tcatttgcag attagaattt ctggcgctta aagagctttt gggagaagaa
     2041 aaggctatga atatagtttc gaataataat aactatacat ggattaggaa gactatattg
     2101 gatagcagtc caattataga tcagattatt gagagatatt ttccgcggat atattagatt
     2161 tataaatttg cgtccgtcat taaaatacct ataataattc tatggcgaaa aataatacta
     2221 aaaacttaat ttatatagta ggtgggctac ttataatagg cgggcttttt gcatggccta
     2281 tcataacaga tgagacgcgg gcgagtgtca aagattggaa taaggcaggg gttgggtgtc
     2341 ttttggggca tcaacaggtg gttagccata tacaccaaca acttttgata actgtagacg
     2401 gcgaggaaga aacaataccg ccaaacactg gtatcgcaag aggttgtatg gctgaaatac
     2461 acgttcacga gggcgaggca aatatcctac acgttgaaac agctgtcgct ggaaaagaga
     2521 tgaagctcgg ccattttttt gctgtatacg acaagccgat aaatagagat gggtacgatc
     2581 ttattatggg cgtaaacgga gaagcgtatg aaggcgatcc attagaattc gttttgcaag
     2641 atcagcaagt tatagagata gaatataata gcgagacagc tcaatagcga gattagctcg
     2701 ttagcaagaa agaaaaaact aaagagctga agagccgagc agcg
//
LOCUS       DATDNZ010000039         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2729459, whole genome shotgun sequence.
ACCESSION   DATDNZ010000039 DATDNZ010000000
VERSION     DATDNZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729459"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VI432_01435"
     CDS             <1..940
                     /locus_tag="VI432_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY4496798.1"
                     /translation="ERTVRPHLFALEKLGVGIKALSDSYEISVKKLKPAEIVLYESGD
                     TATENVIMAASKIDGVTVIKFASANYMVQDLCYFLSRLGIKIDGIGTHILTVYGQSNI
                     NLDVEYSISEDPIDSMLFISAAVTTNSSIDVERCPIDFLDLELLKLEKMGLKYKILRR
                     YKGENGQTNLADVRIFKHKKLKALDEKIYARPFPGLNIDNLPFFVPIASKAHGRTFIH
                     DWVYENRAIYYTELSKLGAQIDLIDSHRVHVTGPINFKPSEIVSPPALRPAAIILIGM
                     LAAPGASVFRNVYTISRGYEDIRSKLNSLGAKIEMF"
     gene            complement(1056..1817)
                     /gene="map"
                     /locus_tag="VI432_01440"
     CDS             complement(1056..1817)
                     /gene="map"
                     /locus_tag="VI432_01440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY4496799.1"
                     /translation="MSNLIKTTDQIERIRVSAKILSKTLKKLRNLVKPGVKLIELDRF
                     AKETILEKGGKPAFLGYRPYGAKEPYPSTLCTSVNDIIVHGRPTNYAVQKGDILKLDL
                     GVNWKGGISDAAITIAVGPITKEGGKLIKTTEKALKEGIRAVKPGHTLGDIGFAIERV
                     ATSQNMKIIDGLTGHGVGIELHEDPVVYNFGKPGSGMVLKENMVLAIEPMLSSRTGKI
                     IQLHDDSFATADGSMSAHFEHTILVTKRGAEILTK"
     gene            complement(1814..2485)
                     /locus_tag="VI432_01445"
     CDS             complement(1814..2485)
                     /locus_tag="VI432_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY4496800.1"
                     /translation="MKAIIFFGPPGSGKGTQAEKTVDRFGFNHLETSKIIEEGFAKAD
                     PNNPEVKAQKERKSRGELISPALFAEWFNKKVEEAFHNKKNIVASGSMRTIEETELVL
                     PKLENLYKPENIIFFIIKISEQESIKRNSERRICEKNKHTIPSGNYNSLYKNIAACPI
                     DGSILKTREDDRPEIIKKRYEIFLKETSPVFDYLKKYGYKLIEINGEQGMDNVFNDIT
                     IHIKQ"
     gene            complement(2519..>3232)
                     /locus_tag="VI432_01450"
     CDS             complement(2519..>3232)
                     /locus_tag="VI432_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEY4496801.1"
                     /translation="GLPSQIRGALVSYSPEMLPTYIAFIALAVIVIAGVVLINEGERK
                     VPVSYAKRIRGNRVYGGASSYLPLKVNQAGVIPIIFAISILLFPQFFGQIISAFSTDL
                     GVKINEWVTNVFNNQLIYGIIYFLLVFAFTYFYISVTFDPKEISKNLQRNGGFVPGIR
                     PGESTAEFLKNIISRITVFGASFLGIIAILPIIVQFLTNAPALTIGGTALLIVVSVAI
                     ETTKQIDSQLTLREYEGIR"
BASE COUNT          912 a          605 c          614 g         1101 t
ORIGIN      
        1 cgaaagaact gttagacctc atctcttcgc gctcgagaaa ctcggagtgg gaatcaaagc
       61 tctgtcagat tcatatgaga tatcggttaa gaaattgaaa ccagccgaaa tagttcttta
      121 tgagtcggga gatacggcta ctgaaaatgt tataatggcc gcttctaaga tagatggggt
      181 taccgtcatt aaatttgcat ccgctaatta tatggttcaa gatttgtgtt attttttgag
      241 tcgtttagga ataaaaatcg atggaatagg aacccatatt ctgaccgtct atggtcaaag
      301 caatataaat ttagatgttg agtattcaat aagcgaagat ccgattgatt caatgctttt
      361 tatctctgcc gccgttacaa ctaattcatc cattgatgtt gaaagatgtc ctattgattt
      421 tcttgatctt gaacttctta aattagagaa gatgggtttg aaatataaaa ttttgaggcg
      481 ttataagggt gaaaatgggc aaactaattt agcggacgtc agaatattta agcacaaaaa
      541 attaaaagct ctagatgaaa aaatttatgc ccgtccattc cctggattaa atattgataa
      601 tttgcccttt ttcgttccta tagcgtcaaa ggcgcatggc agaactttca tccatgactg
      661 ggtttatgaa aatagagcta tttattatac ggagctttct aaacttggag cgcagattga
      721 tttaatagat tcgcacagag ttcatgtaac cggaccaatt aactttaagc cctcagagat
      781 agtttctcct ccagcgctaa gacccgcggc tataatattg ataggaatgc ttgccgctcc
      841 gggagcatca gtgtttagaa atgtttatac tataagcagg ggttacgaag atattcgcag
      901 taaattaaat agtctaggtg ctaagataga gatgttttaa aaatagctca agagcaggat
      961 agttcattaa cgataaaact taagagcaag atagctaatt agctctttag taaaattaaa
     1021 gaactatgct gggtagtcgc tgtctggctg ttttgctatt tcgttaatat ctctgcgcct
     1081 cgtttagtta caaggatagt gtgttcaaaa tgcgccgaca tgctgccgtc ggcggtggca
     1141 aaactgtcat catgaagctg tattattttt cctgttctag agcttaacat cggctcaata
     1201 gcaagaacca tattttcttt taaaaccatt ccgctaccag gtttgccgaa attatacaca
     1261 actgggtctt catgcagctc tattcctacg ccatgacccg ttagaccatc tattattttc
     1321 atgttttggc tagtagcgac gcgttctatg gcaaaaccta tatccccaag ggtatgccct
     1381 ggtttaacgg ctctaatgcc ttctttaaga gccttttcag ttgttttaat aagcttaccc
     1441 ccttctttgg ttattggccc aacagcaatc gttatagcgg cgtcgcttat gccccccttc
     1501 caattaactc ctaaatcaag cttaagaata tcgccttttt gaaccgcgta attagtcggt
     1561 ctgccatgga ctataatatc gttgactgaa gtgcataaag tgctgggata tggctctttg
     1621 gctccataag gacgatagcc taggaatgcg ggtttgccgc ctttttctaa tattgtttct
     1681 ttggcaaatc tgtctagttc tattaatttt actcccggtt tcacaaggtt gcgtaatttt
     1741 ttaagggttt tagacaatat ttttgccgaa actctgatcc tttctatttg gtcagttgtt
     1801 ttaataagat tactcattgt tttatatgaa ttgttatgtc attaaatacg ttatccatcc
     1861 cctgttctcc atttatttct ataagtttat aaccgtattt tttaagataa tcaaagaccg
     1921 gcgaggtttc tttaagaaag atttcgtacc ttttttttat tatctccgga cgatcatctt
     1981 cccgagtttt aaggatagat ccatcaatcg ggcatgcggc gatgttttta tatagagaat
     2041 tgtagttccc gcttggaatt gtgtgtttgt tcttttcgca aatacgtctt tccgaatttc
     2101 tttttatgga ttcttgctcg ctaattttta tgataaaaaa aataatattt tccggtttat
     2161 ataaattttc taatttcggc agcactaatt ctgtctcttc aattgtgcgc attgaacccg
     2221 aagcgacaat gtttttttta ttatgaaacg cttcttcaac ttttttattg aaccattccg
     2281 caaataatgc tggagagatg agttcgcccc tgctttttct ttccttttgg gctttaacct
     2341 ccggattatt cggatcagct tttgcaaaac cctcttctat aattttagaa gtttctaaat
     2401 gattgaaacc aaatctatct actgtttttt ctgcttgagt tcccttgccg cttcctggcg
     2461 gaccgaaaaa aattattgcc ttcatctaca tagtataaat gattaggctc tatattgcct
     2521 atctaatacc ttcgtattct cttagtgtta gttgagaatc aatttgtttt gttgtttcta
     2581 tggcaaccga gactacgatt aaaagggctg ttccaccgat ggtaagcgct ggggcgttag
     2641 tcagaaattg aactattatt ggaaggattg ctattattcc taagaaagag gctccgaata
     2701 cggttattct tgaaataata tttttgagga attccgctgt tgattctccg ggtcttatgc
     2761 ctgggacgaa tccgccgttt ctttggagat ttttagatat ctctttggga tcaaaagtca
     2821 ctgatatata aaaataagtg aaagcaaaaa ctaacaggaa ataaattatc ccgtatatta
     2881 gttgattatt gaatacattc gtgacccact catttatttt aacaccaaga tctgtagaaa
     2941 aagctgatat tatttgtcca aaaaattgcg gaaatagaag tatggatatg gcaaaaatta
     3001 ttggaataac cccagcttga tttactttaa gaggaagata gcttgaagct ccgccataga
     3061 ccctattccc ccttattctt ttagcatatg agaccggaac ttttctttcg ccttcattta
     3121 tgagcacaac cccagcaata actatgactg caagcgctat aaatgctata tatgttggaa
     3181 gcatttctgg cgagtatgaa actaacgctc cgcgaatctg ggatggcaga cc
//
LOCUS       DATDNZ010000040         6873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1933109, whole genome shotgun sequence.
ACCESSION   DATDNZ010000040 DATDNZ010000000
VERSION     DATDNZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933109"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..85
                     /locus_tag="VI432_01455"
     tRNA            12..85
                     /locus_tag="VI432_01455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Gln,seq:ctg)
     gene            298..2031
                     /locus_tag="VI432_01460"
     CDS             298..2031
                     /locus_tag="VI432_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4496802.1"
                     /translation="MKNRQQGFIQITLLIAILAGVIFIGGVGYLGFIKYKTNLAYQKE
                     QESEKVQYKQLQELQQKYLSKTDETLPKPNASEDKVKSALESLPKPTAVFNYHKIIFE
                     LDGQVILKQLNPDKTTILISRKDLEVADPTLNSIQDFHPADFSLSNDNDLIIFNVKKD
                     AFTYHPKTYIFDLMTQKIKYVIDNEDPQYFWSKELSNDNKYLQLYGSTCPCGGELEIV
                     NLETNKAIYNASVGFRGWSPAGYENTLFITSQEYSFNIPAEAWSDSIKLLNIKTDIVS
                     ERILLRGSDQISYSHPYWISDRELGYEMINYSKPFPKEIPSEKWEEYMEIYNKPKITY
                     WKINIDTLKQESIPEPIKQEIEYVKSKIWPYRSFPLSPDKKWELITYGSDLDFNSDIY
                     IVDASETVGLEIAHGNDAIWLPDFNRSSLIETSFFWKVDFKTFIREQDPEIKLCREDG
                     VQPPVDIDSIEYSDLTNDGIPEAIVRASSCLSGTAGPDINNVYKLSISGDPINIKINR
                     VPGEIYDQVWAGYEGHFSYNSENGKLIANFPIYKDGDSNCCPQGGWRKIYYKWDGSGF
                     IFDRFEDVVER"
     gene            2109..2651
                     /locus_tag="VI432_01465"
     CDS             2109..2651
                     /locus_tag="VI432_01465"
                     /inference="COORDINATES: protein motif:HMM:NF017820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY4496803.1"
                     /translation="MNFESKIKEIILSDERLMKILRSARELNLNDWFVAGGAIRNSVW
                     DILSGFQNHTELNDIDLVYFDRNKANDEYDRNLSEQMKKKLSQFNWEVENQAKYESKG
                     EVFNKTEDGIARWIETPTCIGVRLENDGLITVCAPNGLEDLFTGNIRINPKWPKPVYF
                     EERKNKKRWDKVWSIARYTN"
     gene            2846..3352
                     /locus_tag="VI432_01470"
     CDS             2846..3352
                     /locus_tag="VI432_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4496804.1"
                     /translation="MKILITGLPGTGKSTISKALREKGVLSIDFSDVSGMCFWQNKET
                     KEKLKYSPIQTREWFEFNERICDLEKLKEMLNQHENIIMTGLAMGNQKEYLPLFDKVI
                     LLRCSPNVIVYRMQTRVAPYGKTKTERDEAIGKQKEFDDLILSCGAIPINNDGPISAV
                     IDNIISKL"
     gene            3571..3999
                     /locus_tag="VI432_01475"
     CDS             3571..3999
                     /locus_tag="VI432_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496805.1"
                     /translation="MKNYWIWILIAIVLVGGYFYFTNKPVEDSDTINSMEVMLSALND
                     SGVSGSATLTEQDGDLMVALALTGNVAGTVMPAHIHDGVCPGVGAVKYPLTSATDGSS
                     TTIIEGVTLANLESMLPLAINVHLSADQISTYVACGSLAF"
     gene            4096..5574
                     /locus_tag="VI432_01480"
     CDS             4096..5574
                     /locus_tag="VI432_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4496806.1"
                     /translation="MPKDEVIIRFDKATFEYGHNRPILDEADFSIREGSKITLMGQNG
                     AGKSTIFQLIGGNLKPESGKVIVKQELTFAISKQVIPRDELDLTVREFFEKVFSRKMY
                     DIEPRINKILEVVNFPVSHERIIKSFSGGQQARLLLAQALIQNPDILLLDEPTNNLDK
                     DGIEHLTNFIKNYEKTCIVISHDADFLNSFTEGVLYLDIFTRKIEQYVGNYFDVVREI
                     KNRLERERMKNVRLEKDIAHRKEQAGFFAQKGGHMRDVAKKMRDKIDELSEEIVDMRR
                     EDKTIRKFTIPYQEDLGGQLLKLTSVNVIKGHKSSSKKVEVVLKKKEHLLLKGPNGIG
                     KTSLLERLSTGHAKGEPFDKAQDKAVADGVRIGYYRQDFGTLDFNETVYNTLKNAMWQ
                     GGEQELRSAASGFLIDAEILKSKVGALSEGQKGLVAFASLVLMKPGLLILDEPTNHIN
                     FRHIPVIAEALDKYEGAMILVSHVPDFVEKIRIDQELDLGSL"
     gene            complement(5614..6852)
                     /locus_tag="VI432_01485"
     CDS             complement(5614..6852)
                     /locus_tag="VI432_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY4496807.1"
                     /translation="MVRSKDDITAGDGAKHDIMQGKAAIANQTTVNVFNLLKECGIPL
                     AFIQGLDETSFLARNCRMIPLEVVVRREAHGSYLKRNPHLEKGHIFPQLICEFFLKTS
                     GKEWYGRAIPEDDPLIVWGNNREANLYLPKKPMWEQKSFMTVDPAEIFSEDELNSEDI
                     FNEDPQGIERMTHIAKATFFVLEKAWQILGRRLVDFKVEFGIDHNGNLVLADVIDNDS
                     WRVIEDGEYIDKQFYRDGGALDEVTAKYAHVRDLTGLFGIPHQQLILWRASDKDNFGP
                     LKEAFVEYGSLEECQITEITRSLHKEPVRAYLDLAKAVRNVPDSVLIALVGRSNGAGP
                     TLSAQTSIPVITVPATGKDFPEDLWSSLRTPSETPVATIMEPANAILQALQILALRNP
                     LLYSRLRIKQERRLMNIAAL"
BASE COUNT         2167 a         1206 c         1523 g         1977 t
ORIGIN      
        1 atccattgta ttgccggatg gtgtaaaggt agcacgtcgc tctctggaag cgaagattgg
       61 ggttcgagtc cctgtccggc agccagcaaa aaaattttct ccgcatttct gattttgcgc
      121 gcgccggact ttttcttcta aaaggaaaag aaaacttttt tgctggctgg cgagcggagc
      181 gagcaacggc ggggcgactt cattcgtcgg gatcgtttgg tagaaagttc ggattttgtt
      241 caggagagac agccatggaa tacaaataag gatacttcca tagtatcctt attctatatg
      301 aaaaataggc agcaaggatt tatacaaata actcttctaa tagcaattct agccggagtt
      361 atttttatag gtggggttgg ctatcttggt tttattaaat ataagaccaa tctagcttat
      421 caaaaagaac aagagtcaga aaaagttcag tataaacagc tccaggaact acaacaaaaa
      481 tatttgagca aaactgatga aactctccct aaaccaaacg catcagaaga taaggtaaag
      541 agcgcattag aatctcttcc aaaacctaca gcagttttca attaccataa aattattttt
      601 gaattagacg gacaagtcat ccttaaacaa ttgaatccag acaaaaccac tattttaatc
      661 tcgagaaaag acttagaagt agccgatccc acgcttaatt ctattcaaga ttttcatcca
      721 gcagatttct cattatccaa tgataatgat ctcattattt ttaatgttaa aaaggatgct
      781 ttcacgtatc atcccaaaac atacatcttt gatttaatga cccagaaaat caaatatgta
      841 attgataatg aagacccgca atatttctgg tccaaagaat tgtctaatga caataaatat
      901 ttacaacttt atggatctac ttgcccgtgc ggaggtgaac tggaaatagt gaatctcgaa
      961 acaaataaag ctatttataa tgcaagtgta ggctttaggg gttggtcccc ggctggttat
     1021 gagaatactc tttttattac ttcacaggaa tactcattta atatacctgc agaggcttgg
     1081 tcagattcaa taaagcttct aaacattaaa accgacatcg tttcagaaag aatactttta
     1141 agaggctctg atcaaattag ctattctcac ccttattgga taagcgatag agaattgggg
     1201 tatgaaatga tcaattatag taaaccattc cctaaggaaa taccttctga aaaatgggaa
     1261 gaatatatgg agatttacaa taaaccgaaa ataacctatt ggaaaattaa catagacaca
     1321 ctaaaacaag aatccattcc ggaaccaatc aaacaagaaa ttgaatatgt aaaatctaaa
     1381 atttggcctt atagatcatt ccctctttct ccagataaaa aatgggaatt gataacatac
     1441 ggaagcgatc ttgatttcaa tagcgatata tatattgtgg atgcgtctga aacagtagga
     1501 cttgaaattg ctcatggcaa tgatgcaata tggcttcccg attttaatcg atcgtctctt
     1561 atagaaacta gtttcttttg gaaagtagat tttaagacat ttatccgaga acaagatcct
     1621 gaaattaagt tatgcagaga ggatggtgtc cagccacccg tagatattga ctcaattgaa
     1681 tatagtgacc ttactaatga tggaatcccg gaagctatag ttagggcatc aagctgtctg
     1741 tctggaactg ccggaccgga tataaataat gtttataagc tttctattag tggagatcct
     1801 ataaatataa aaataaatag agttcctggt gaaatctatg atcaagtttg ggccggatat
     1861 gaagggcatt tctcatacaa ctcggagaat ggtaaattaa ttgctaactt cccgatttat
     1921 aaggatggag attcaaattg ttgtccgcag ggtggttggc gtaaaatcta ttacaaatgg
     1981 gatggatctg gattcatatt tgatagattt gaggatgtag tagagcgata aaaagttcca
     2041 acatcgtccc acaacctcag catcagtaaa cgcatcatcg agagaccgta gagtaggtga
     2101 attagcaaat gaattttgaa tctaaaataa aggaaattat tctctcagac gaacgtttaa
     2161 tgaaaatact tcgctctgcc agagaattaa atttgaatga ttggtttgtc gctggcgggg
     2221 caatacgtaa tagtgtctgg gatattttaa gcggatttca aaatcatact gaattaaatg
     2281 atatagatct ggtctatttt gatcgaaaca aagctaacga tgaatatgat agaaacttaa
     2341 gcgaacagat gaagaaaaaa ttatcacaat ttaattggga ggttgaaaac caagccaaat
     2401 acgaatcgaa aggagaggtt ttcaataaaa ccgaagacgg aatcgctcgc tggatagaaa
     2461 cccctacttg cattggagta agattggaaa atgatggctt gataactgtt tgcgcgccaa
     2521 acggattgga agatcttttc accggcaata tcagaatcaa tcccaaatgg cccaagccgg
     2581 tttatttcga agaaagaaaa aataagaaaa gatgggataa ggtctggtca atagctagat
     2641 atacaaacta gaccgagccg ggtcgttttt gagctggaac taggacctag aaacccttat
     2701 actgattagt attacaaagt tctaggccgg agaacacagc cgacgaacga aggtgagaag
     2761 ctactgaggc taccaagccg aagtacatag tcgtcaatca gcttattatg gagcgaccgg
     2821 aattctatat agtatcagaa tatttatgaa aattttaatt accggattgc ctggcactgg
     2881 taaatcaact atatccaagg ctttgcgaga aaagggtgtt ttatcaattg atttcagcga
     2941 tgtgtctgga atgtgttttt ggcagaataa ggaaacaaaa gaaaaattaa aatactcacc
     3001 aattcaaact agagaatggt ttgaatttaa tgaaagaata tgtgatttag aaaaactgaa
     3061 agaaatgtta aatcagcatg aaaatataat tatgactggc ttggcgatgg gaaatcaaaa
     3121 agagtatctt cctctttttg ataaggtgat acttctgaga tgtagcccaa atgttattgt
     3181 ttatcgtatg caaactagag tagcgccata tggtaaaaca aaaacagaac gggatgaggc
     3241 tattggtaaa caaaaagagt ttgacgattt aatcctttca tgtggtgcaa ttccgataaa
     3301 taacgatgga cccattagcg ctgtgattga taatattatt tctaaattgt agctcgatcg
     3361 aaatccttaa ttctcttcag taagttcagg gcaagtcgct atactgaaat tgtccaaaca
     3421 aaacccttta acggcctact tggtggcggt tccagaccga gagatgcaga tatatttatt
     3481 ccttgttata tttgagatac gttttataat atttttatac tgaattttat tcagctttta
     3541 aaaggtcgat taataaatat aattttgaaa atgaaaaatt attggatatg gattttaata
     3601 gcaatagttt tagttggcgg atatttttat ttcactaata agcccgtaga agatagcgat
     3661 accatcaact caatggaggt aatgctttct gctttgaatg attctggcgt atcgggttca
     3721 gcaactctta ccgagcagga tggagatctt atggttgctc tcgctttaac cggcaatgtt
     3781 gcggggactg ttatgcctgc ccacattcat gatggagttt gtcccggggt tggtgctgtt
     3841 aaatatccat taacttcagc gacagacggc tcatcaacta caattataga gggtgttacc
     3901 ttggctaatt tagagtctat gttgccgctt gcgattaacg ttcatttgtc tgctgatcag
     3961 attagcactt acgttgcttg cggaagtttg gctttctaag aatctatatt cttataaaaa
     4021 ataccgttgt aaaacggtat tttttattta tgaatagaat tgataaatct ctataaagca
     4081 tttagactat cccctatgcc gaaagatgag gtgataatta ggtttgataa ggcaaccttt
     4141 gaatatggac acaataggcc aattttagat gaggcggatt tttctattcg cgaagggtca
     4201 aaaatcacgc ttatgggcca aaatggcgcg ggaaaaagca ctatattcca gcttattggg
     4261 ggaaacttga agccagaatc gggaaaggtg atagtgaaac aagaattaac ttttgctata
     4321 agcaagcagg ttattcctcg cgatgagttg gatcttacgg ttcgcgaatt ttttgaaaaa
     4381 gttttctctc gtaagatgta tgatatcgag ccaaggatta ataaaatatt agaagttgtt
     4441 aattttccag tatcacacga gagaatcata aaaagtttct ccggtgggca gcaggcgcga
     4501 ttgcttctgg cgcaagcttt gattcaaaat cctgatattc ttctactgga tgagccgaca
     4561 aataatctgg ataaggatgg aatagagcat ctaacaaatt tcataaaaaa ttatgaaaag
     4621 acttgtatcg tgatttctca tgacgcggac tttttgaatt cgtttaccga aggagtttta
     4681 tacctagata tttttactcg taaaattgaa cagtatgtcg ggaattattt tgacgtggtg
     4741 cgcgagatta aaaatagatt ggaaagagaa agaatgaaga atgtccgttt ggaaaaagat
     4801 attgctcatc gcaaagagca ggctggattt ttcgcgcaga agggagggca tatgagagac
     4861 gtggcgaaaa agatgagaga taaaatagat gaactctccg aggagattgt tgatatgaga
     4921 agagaagata agacaataag aaaatttact atcccctacc aggaagacct tggcgggcaa
     4981 ttattaaaat taacttcagt gaatgttata aaaggacaca aatctagttc caagaaggtt
     5041 gaggtcgtat taaagaaaaa agaacattta cttttgaaag gaccaaatgg aattggaaag
     5101 acatcccttt tagagagact ctcaaccgga cacgcaaagg gagagccctt cgacaaggct
     5161 caggataaag cggtcgcaga tggagtgcgt atcggttatt atagacaaga ttttggaacg
     5221 cttgatttta acgagactgt ttataacaca ttgaaaaacg ctatgtggca gggcggagag
     5281 caagagcttc gatctgcggc gagcggtttc ttgatagacg cggagatttt aaaatcaaaa
     5341 gtcggagcat tgtccgaggg gcagaaggga ttggttgcgt ttgcctcttt ggttttgatg
     5401 aaaccggggc ttttaatatt ggatgaaccg actaatcata taaatttcag acatattccg
     5461 gttattgccg aggcattgga taaatacgaa ggcgcgatga tattggttag tcatgtgccc
     5521 gattttgtag aaaaaataag gatagatcag gaattggatt tgggcagttt ataaaaaata
     5581 gacgcagctt aagatgttgc gtcttttttg tttttatagc gctgcgatgt tcatcaggcg
     5641 cctttcttgc tttatgcgga gccttgagta gagcaaggga ttccgaaggg caagaatttg
     5701 aagggcttgg aggatggcgt ttgccggctc cataatcgtt gcaaccggag tttcgcttgg
     5761 agtccgaagc gatgaccaga ggtcttccgg gaagtctttt ccggtcgccg gcacagtaat
     5821 tacggggata ctggtttgcg ccgaaagtgt gggtcctgcg ccattgcttc tgccaacaag
     5881 agctatgaga accgaatccg gcacattccg aactgcttta gcgaggtcta ggtatgcccg
     5941 gacaggctct ttgtgaagag accgcgtaat ttccgttatc tggcattctt ccagtgaacc
     6001 gtattctaca aaagcctctt tgagcggacc gaaattatct ttgtcggacg ctctccagag
     6061 aatcaattgc tgatgcggta ttccaaagag gccggtcaga tcgcgaacat gggcgtattt
     6121 tgctgttact tcgtcaagcg ctccgccatc gcggtagaac tgcttgtcga tgtattcgcc
     6181 gtcttcgata acgcgccatg aatcgttgtc gatcacatcc gccagtacaa ggtttccgtt
     6241 gtgatcgatt ccaaactcga ccttaaaatc aaccagacgt cggccaagaa tctgccaggc
     6301 tttttcaaga acaaagaatg tggctttcgc gatatgagtc attctctcga tgccttgggg
     6361 atcttcattg aagatgtctt cggagttgag ttcgtcttcg ctgaaaatct ccgctggatc
     6421 cactgtcata aaggattttt gttcccacat cggtttcttg ggaagataga gattggcttc
     6481 tctattgttg ccccagacga taagcgggtc atcctcggga atggcccttc cataccattc
     6541 ttttcctgaa gtcttcaaga agaattcgca aatgagttgc gggaagatat gacccttttc
     6601 aaggtgagga ttccttttga ggtatgatcc atgagcttca cgacgcacga cgacttcaag
     6661 cgggatcatg cggcaatttc ttgccaggaa acttgtttcg tctaatcctt ggatgaaggc
     6721 aagtggaatt ccacattctt tcaaaagatt gaagacgttg accgtcgttt gattggcgat
     6781 agccgcttta ccttgcataa tgtcgtgttt cgcgccgtca ccggcggtaa tatcatcctt
     6841 agaacggaca atgacttgga gcccgtcggg cgg
//
LOCUS       DATDNZ010000041         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3500576, whole genome shotgun sequence.
ACCESSION   DATDNZ010000041 DATDNZ010000000
VERSION     DATDNZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500576"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VI432_01490"
     CDS             complement(<1..440)
                     /locus_tag="VI432_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496808.1"
                     /translation="MKQIQEILTREDIQRLYDEGKTEEALRQIGLVLPEWDDPEEVEH
                     LYAIKAWCHYRRKEYDLCRQAIVNAGNGVKAKECLAQLTAYVDKDDDLLAQIVLSLPD
                     NVGVANAYMIRARDKDSVITHEGVMEVVRRLLGSDEISATNLVN"
     gene            complement(507..1748)
                     /gene="murD"
                     /locus_tag="VI432_01495"
     CDS             complement(507..1748)
                     /gene="murD"
                     /locus_tag="VI432_01495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY4496809.1"
                     /translation="MLIDELKKYKKILILGYGIEGKATHEFLKKNMPEAEIGIADAKD
                     GENYLNKQKDYDLVIKSPGISKKFLTQKYTTATNIFFANAKAPIIGITGTKGKSTTTS
                     LIYEMFKEGGYDARLLGNIGKPMIDALSQPVSRKTIYVLELSSYQLDDLQHSPHKSLF
                     INIFPEHLDYHGSFEEYKKAKANIGRFMTEKDDFVYNAKYKELVEIAKSIKAKPVKVV
                     DELPFEPKTALLGKHNIENIKAAYTVAKLMDVSDEDIIRAVKNFKPLPHRLEFVGNFH
                     GIDFYDDAISTTPESTIQALEAIPNIDTIFLGGTDRGYDFSSLAGKLISSEVRNIVLF
                     PDSGTKIKEIIEVNCQMLNVKCSFNFFETRDMKEAVKFAYKNTDVGKVCLLSCASPSY
                     SVWKNFEEKGDLFKKYVMELD"
     gene            complement(1842..3188)
                     /locus_tag="VI432_01500"
     CDS             complement(1842..3188)
                     /locus_tag="VI432_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496810.1"
                     /translation="MNKPDSKRVFTFESYDFNPKSKEIHFNYRLSGKGEDIAFQEKLI
                     FPKNLSWRNAPDKVLHSALESLHIILGISYWKLYCPKKIVLNTTFLSKEQANFFNRVY
                     TLGLGEFFYKNKIDFRNLIKFPSSSKLKTKNYQLKTNKSRALLLFGGGKDSYVSAEIL
                     KKDKKNFDYFTVITGGREVLQNLAPKGTLTIERFLDPKLFELNKSGKVYRGHIPVSAI
                     LASIGELAAILYDYRYVIASNEKSANYGNVKYLGREINHQWSKSIEFEEMMQNYSHRY
                     ITPSVTYFSLLRPLYEIKIVEIFTRFGKKYFNKFSSCNTNFKIGERAPKRRWCLNCPK
                     CAFVFTMMAPFISKKKLIDIFGGNAFNKKNLLNIFKELLGIRAIKPFECVGTPDEMRL
                     AMSMILIREEFKNDFIIKKLGSKIKHPSKKVEKEIMGLSGDHMIPKEFKKAIPIYE"
BASE COUNT          931 a          727 c          554 g         1087 t
ORIGIN      
        1 ttgttcacga ggttcgtcgc cgagatctcg tccgagccaa ggaggcgacg aacaacttcc
       61 atcacccctt cgtgggtgat gacgctgtcc ttgtcgcgtg cccggatcat gtatgcgttc
      121 gccacgccca cgttgtcggg cagactcaac acgatctgcg cgagcaggtc gtcgtccttg
      181 tccacgtagg ccgtcagttg ggccaagcat tccttggctt tcacaccgtt gcctgcgttc
      241 acgatcgctt gacggcaaag atcgtactcc ttgcgccggt agtggcacca agccttgatg
      301 gcgtagagat gctctacttc ctccggatca tcccactctg gcagaacaag accaatctgc
      361 ctgagcgctt cttcggtctt tccctcgtca tagagacgct ggatgtcttc tctagtcaga
      421 atttcctgta tttgcttcac actccttatt gtcaaaggac catctgtcct aagctgagat
      481 aaattagcac ataaatataa attatgtcaa tctaactcca tcacgtattt tttaaacaaa
      541 tctccttttt cttcaaaatt tttccaaaca gaatagctgg gcgaggcgca agataaaaga
      601 caaacctttc cgacatcggt gtttttatag gcgaatttca ccgcctcttt catatctcga
      661 gtttcaaaaa aattgaaact acatttaaca tttaacattt ggcaattgac ttctatgatt
      721 tctttaatct tggttccgga atccggaaac aaaactatat ttctgacttc cgagctaatg
      781 agctttcctg ctaatgagct aaaatcatat cccctgtctg ttccgccaag aaatattgtg
      841 tctatatttg gaatcgcttc taacgcctga atagtggact cgggcgtggt ggaaatcgcg
      901 tcgtcataga aatcgatacc gtgaaaatta ccaacaaatt ccaatcgatg cggcaggggt
      961 ttaaaatttt taaccgctcg tataatatct tcgtccgaaa catccatcaa tttagcaacc
     1021 gtatatgccg cttttatgtt ttcaatatta tgctttccca aaagcgcggt tttcggctca
     1081 aacggcaatt catcaacaac tttaaccggc tttgctttta tggactttgc aatttccacc
     1141 aactctttgt atttcgcgtt atacacaaag tcatccttct cggtcataaa cctacctata
     1201 ttcgcctttg cttttttata ttcctcaaaa ctgccatggt aatcaaggtg ttccggaaaa
     1261 atatttataa aaagcgattt atgcggggag tgctgcagat cgtcaagctg ataactggaa
     1321 agttctaata catagattgt ctttctactt accggctgac tcaaggcatc aatcatcggc
     1381 ttgccgatat ttcccaacaa gcgcgcatca tatccgccct ctttaaacat ttcgtagatc
     1441 aaacttgtag ttgtactttt accctttgtt ccagtgattc cgattatcgg cgcttttgca
     1501 ttcgcaaaaa aaatattagt cgcagttgta tatttttgag tcaagaattt ctttgaaatg
     1561 cccggcgact ttattaccag atcataatct ttctgcttat taagatagtt ctcgccatct
     1621 ttcgcgtcgg ctatgccgat ttctgcctct ggcatattct tcttcaaaaa ctcgtgagtc
     1681 gccttgcctt ctatgccgta tcccaaaata agaattttct tatatttttt taattcatct
     1741 attagcataa atgctcaata taatactaat atacaaattg tgctaatgat acgaatggca
     1801 aacgaatagg attcattcgt attattcgtt gcattcgcat gtcattcgta tattggtatc
     1861 gctttcttga attctttagg aatcatatga tctccggaaa gtcccataat ttctttttca
     1921 acttttttgc tcggatgttt tattttagac cctagctttt tgataataaa atcattctta
     1981 aattcttctc taattaaaat catagacatc gcaagacgca tctcatccgg cgtacccaca
     2041 cactcaaaag gttttatagc ccttatccct aaaagctctt tgaatatatt tagcaggttc
     2101 tttttattaa acgcattgcc tccgaatata tcaattaatt ttttctttga gataaacggc
     2161 gccatcattg taaaaacaaa agcgcatttg ggacagttaa gacaccatct cctcttcgga
     2221 gctctttctc ctattttaaa attggtatta caacttgaga atttattgaa atatttcttg
     2281 ccaaaacgcg taaagatctc aacgatctta atctcatata gcggacgcaa caaggaaaag
     2341 tatgtaaccg aaggtgttat atatctatga gagtaatttt gcatcatctc ctcaaattcg
     2401 atagatttac tccattgatg attaatctct ctgcccaaat atttaacatt tccataattc
     2461 gcgcttttct cgtttgatgc gataacataa cgataatcat ataaaatggc cgctaattca
     2521 ccgatggacg caagtatagc agacacagga atatggccac gatacacctt accactctta
     2581 tttaactcaa ataatttcgg gtctagaaat ctttctatgg ttagtgttcc ctttggagct
     2641 aaattttgaa gcacctctct gccgccggta attacagtaa aataatcaaa attctttttg
     2701 tcttttttaa gaatctcggc cgaaacatat gaatccttgc caccgccaaa taaaagcaga
     2761 gctctacttt tattagtttt tagttgatag tttttagttt ttagtttaga gcttgatgga
     2821 aatttgataa gattgcggaa atctattttg tttttataaa agaactcgcc caaacccagc
     2881 gtataaactc tgttaaaaaa gttagcctgc tctttactca aaaaggttgt attcaaaact
     2941 atctttttcg gacaatacaa cttccaatag cttataccta aaattatatg caatgattct
     3001 aacgctgaat gcagaacctt gtctggcgca tttcgccaag aaagattttt agggaatatc
     3061 agtttctctt gaaaagctat atcttcaccc tttccagaca gtctataatt gaaatgaatc
     3121 tctttacttt taggattaaa atcataactt tcaaaagtaa aaaccctttt agaatctggt
     3181 ttattcatac ggcttgaatt atagttgaat caatagttat aataaagctt gtctaaaata
     3241 agcagccgta gtgtagcccg gttaacacgt ctccctgtca cggagaagat cgccggttc
//
LOCUS       DATDNZ010000042        18957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1171863, whole genome shotgun sequence.
ACCESSION   DATDNZ010000042 DATDNZ010000000
VERSION     DATDNZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171863"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1278
                     /locus_tag="VI432_01505"
     CDS             103..1278
                     /locus_tag="VI432_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY4496811.1"
                     /translation="MKKRIYLDYAASTPVLKEVDAVMKSVARGKIGNPGSIHSFGQEA
                     MAVIDKSREDIADAIGADFSEIVFTSSATEANNLAIRGVVKKAASKNGKNLNIISSSI
                     EHESILDTFNDLKKDGINAFHAPVSKNGIVDLKQIKKLINSETALVSVMYANNEIGVI
                     EPISEIAKLIKDFRIANRSKYPLFHVDAVQAFQFLNCDVEFLGVDLMTISSHKIYGPK
                     GIAALYIKELGKGSVIEPILTGGGQEFGMRSGTESPALIAGFAKAVKIAVRDRKRQFQ
                     ISKGLRDHLWKKIKEGFPKSKINGSLTNRLPNNLNIYLAGRNAEDLVIGLDLAGIAVS
                     SGSACRARSLEPSYVIEALGYPKERAMSSLRFSLGRQSTIREIDMVIKQFLKLINKK"
     gene            1281..2537
                     /locus_tag="VI432_01510"
     CDS             1281..2537
                     /locus_tag="VI432_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4496812.1"
                     /translation="MKKNILTLIIVLSFVGGIIVSDSVISNTSAGIWQDIVDKVSPFF
                     GGNNVKDKPADIENVSEPYISPSSYEDAVIKAVEDGSKSVVSIIISKDLPIIEQCPNN
                     PFGNNPDLFDFFGRDFGFQFYQPCEKGTKYQEVGGGSGFIISKDGLIVTNKHVVSDEK
                     ADYTVLINNGNKYKAEVLARDPVLDLAIIKINAPEGLLYPAKLGDSDSIKLGQTTIAI
                     GNALGEFRNTVSIGVVSGLARSITASNGASLEKIDDLIQTDAAINKGNSGGPLLNLKG
                     EVIGINTAIVAGAQNIGFAIPVNQAKRDIESVKKTGTIKTPYLGVRYFVITPELALEQ
                     KLTVEYGALVRGNESGPGVIPDGPADKAGILSEDIILSVNSEKLDGDKTLVSIIQKYD
                     IGDKLNLGIKRGDNDITLDVVLEERP"
     gene            2641..5274
                     /locus_tag="VI432_01515"
     CDS             2641..5274
                     /locus_tag="VI432_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4496813.1"
                     /translation="MNSFNRFTIKAQEALQNAQDLASAQNHGEFKALHLLSALIADDS
                     SLVRPIITRLGASLDSIDRTINKELKELPKIFSSTSLGQLYVSQEVMKILDRAAKAAT
                     ANKDEFISCEHLLLGILDSDSAAKNILETSGIRRESAMRTLSGLRGSTRVTDETPESK
                     FQVLEKYGINLSEKAAEGKLDPVIGRDEELRRVIQILSRRTKNNPVLIGEPGVGKTAV
                     VEGLAQRIVSGDVPEILKDKEVVSLDLGSMVAGTKFRGEFEDRLKAFIKEIQNSAGRI
                     ILFIDEIHMIVGAGAAEGAVDASNLLKPALARGELHAIGATTLREYQRYIEKDAALER
                     RFQPVLVEEPSIEDSVTILRGLKPKYEIHHGLRISDEAIVSAVNLSARYISDRFLPDK
                     AVDVIDEASAARKIEAESLPIEIDKIRREITNLEVEKAAINGDSQKTDSKKLAEINKK
                     LKTAKTRNETLSSEWQGEKLIFEKLNNLRSRVESLRRERDVAEREGQLERVAQIIYGE
                     LPEAEKEYRTYEKKYFSIGKKKNNKNKFLKEEVDAEDIAKVIARWTGIPVSKILEIEE
                     EKLKRVETFLKKRVVGQEEAVKSVSSALRRARAGLAAPDRPLASFMFLGPTGVGKTEL
                     ARSLSEFMFNDEKSLVRIDMSEYMEKHAVARLIGSPPGYVGFEEGGQLTEIIRHKPYS
                     LILFDEIEKAHPEVFNILLQVLDNGRLTDGKGRMVNFKNAIIIMTSNVGSEFFKEMSK
                     LGFNMSSDEAKDKEDDYKSRIKSSLKDKFRPEFLNRIDDIIIFKSLNREDIAEIVEIQ
                     LKDLKNRVSERGIKLVIEPNVKKYIIENGYDAEYGARPIKRLIQKTITDKLADTIIGG
                     GIKHGARVKIVSEGSNIKVSV"
     gene            5219..5950
                     /locus_tag="VI432_01520"
     CDS             5219..5950
                     /locus_tag="VI432_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496814.1"
                     /translation="MALGLKLFLKGPISKLAFRSSVVAIAVLALRLTPFGFLPTSFFL
                     ILFSILYLQPNLNNGRFLIPFSVLLFFIFFMPLGEIRYYELYIAGLSGILFFMLSGVK
                     NVIFINRNFIYRISHGALILLILSFFFKYPPNLYIQFMTFLLIFFLYRELYLMHRLDQ
                     KRINLLSAIMALLVSEIIWAIFLIPTSFSGLLFMSAAPIFLVDDILINYLRGERLHNI
                     FIRDFSLFIIFIIFVAGFINFGLPR"
     gene            6024..7547
                     /gene="rny"
                     /locus_tag="VI432_01525"
     CDS             6024..7547
                     /gene="rny"
                     /locus_tag="VI432_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY4496815.1"
                     /translation="MIYQILGGIGVLLLGALIGYFVRRFILIKQAALLEDKLKDNVTR
                     AKNEAQDIVLEAKNKAVSILEESKKEEKERKSSLDRLEERLLKKEEDLSRKENGFHEE
                     KEKLINELKRIEESEKEVEKSKGEVTKKLAELAGLTAEQAKERLMKDVEDQNKEDLTS
                     ALHKIFNERKEEIEKKSLEIMTTAVQRYARSHVSDITTSVFNLPSEDLKGKIIGREGR
                     NIRTLERLAGVELIVDETPDSLIISSFDPLRREIARLTLEKLIKDGRIQPAKIEEKVE
                     EAKSELDKRMFEIGEAAAYELSILDFPKEILQLLGRLNFRTSFGQNVLTHSVEMAHLS
                     GMMASELGANVEVAKRGALLHDIGKAIDHEVQGTHVELGRKILKKYGVSEEVIAAMQS
                     HHEEYPFSTPESFIVTAADALSAARPGARRGTLENYIKRLEDLERIATSFPGVKNSYA
                     IAAGRELRVFVVPEKIDDFKALQLARDIANKIQSELRYPGEIKVNVIREIRAVEYAK"
     gene            7639..7914
                     /locus_tag="VI432_01530"
     CDS             7639..7914
                     /locus_tag="VI432_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4496816.1"
                     /translation="MNKAQLIETVMTSAGLETKKQAESAVEAVFDTVTKVLGRGEEVA
                     IAGFGTFKTSKRAARAGINPKTGEKIQIAAKTLPKFKAGKLLKEAVQ"
     gene            complement(8008..8865)
                     /locus_tag="VI432_01535"
     CDS             complement(8008..8865)
                     /locus_tag="VI432_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496817.1"
                     /translation="MNKAIKMGIMAILAFALAPSITHATEEGLKEARNRIDSSYDELL
                     SLAGDSDLNPISNSTEDNEAKVLIIKSVVKDAINLSIQEVVALKKDMETLPQFSAKSR
                     EQELKSVFAEELSTFAQFYNIKMKELNRIGTLEDGRALAEEIITYREKSYNPRISQLL
                     EFLLLFYNENVIKTAEEKTERISKDVEKLEKLGFLKTDSFLKDFDTIASLINQAYELK
                     NQAKIFIINPALKLNVSDLSEKSKEKEDVTHIPEPRLLMEDSLGKIKIVYDIFIDVSK
                     SIRKTLGLK"
     gene            complement(8871..9401)
                     /locus_tag="VI432_01540"
     CDS             complement(8871..9401)
                     /locus_tag="VI432_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496818.1"
                     /translation="MTDQDLIKRLSKINKIKPSEDYALISRSILLNKEAGLDNDLVSR
                     LNMLRAIQPRTEFALKTKDAIHAIAAKPPLMDLGFNIKNMGTIPRLIHQSLSFSMAIA
                     LSIIAITVITTTPNIKEAFLPELSALENEDLAIEADNLTKDINIKLLEAEYFASSANK
                     TTMALSEALTKTGSLK"
     gene            complement(9398..9940)
                     /locus_tag="VI432_01545"
     CDS             complement(9398..9940)
                     /locus_tag="VI432_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4496819.1"
                     /translation="MLEDEKKIIRKAREGEANAFGLLYDHYLERIYRFVLLKVSHREE
                     AEDLTHLAFLKAWERIGEYQDQGFPFGSWLYKIARNTIIDHYRSSNGRKTEITLDYSL
                     EIIPSEDDHLDKSVDNAIEIQTVMDAIKKLKPIEQDVVIMRFIDEMSNEEVADAVGKT
                     IGAVKLIQHRALRKIKQELK"
     gene            complement(10055..11092)
                     /locus_tag="VI432_01550"
     CDS             complement(10055..11092)
                     /locus_tag="VI432_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEY4496820.1"
                     /translation="MNIKIKTIKDRFVWDNFIKEVKPNTFLHSWEWGQFNEEMKGKIY
                     RLGAFDNEELISVALIIKTDARRGSFIFCPHGPIIKISKVENLIDEVLSKLTDKLKEI
                     ANNEGCSFIRISSLLTDTPNNRKIFKNLGFINAPIHMHPEKAWVIDINKSEEAILKDM
                     RKTTRQLIKKAEQEGVQIEILMSEKNAVQRFEKIYETTSERQGFTPFSDKYIENEFKV
                     FSQNNKSEILIAKYKNQDLSGAFIIFDENGAYYHHGASNQNFPKIPASHLLQWKIIQE
                     ARRRNCKKYNLWGIAPNNKNNHPWKGLTLFKTGFGGYSEDYVPAQDLPINSKYWLNYF
                     VESLRRIKRGL"
     gene            complement(11076..12344)
                     /locus_tag="VI432_01555"
     CDS             complement(11076..12344)
                     /locus_tag="VI432_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4496821.1"
                     /translation="MLFNKYKPILISLSPNAESDDIKLAIKLLFSPRKWLKGDAINQL
                     ISELKKIIKVDFIYITNSGRAALYTILKALGIKNGDEILLQAYTCVAVANSIKWTGAK
                     PIYVDINPQTLNMSTDDLEKKITKDSKAIIIQHTFGLPADIDGIKKIAEKHNLKIIED
                     CAHSLGAEYKNAATGSFGDASFFSFGRDKIISSVYGGAIATNNSDLSEKINKELNHLN
                     NPKKTWIKQQLLHPIIFNMAIKLYGILKIGKIIIEASKKLHLTSMAVYKLEKTGGKPE
                     FISYKMPNAIAELALNQIRKIKRFNEHRKRIARIYDERLEKLNNNGVILPTREGNSVY
                     LRYTIQTDQAETIIAKAKKENILFGDWYRPPIAPQGVDQKRAGYIPGSCPKAELAAQK
                     SLNLPTGPNILEEDANRIADFILQQYEYKN"
     gene            complement(12331..13983)
                     /gene="murF"
                     /locus_tag="VI432_01560"
     CDS             complement(12331..13983)
                     /gene="murF"
                     /locus_tag="VI432_01560"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY4496822.1"
                     /translation="MIVEQILIAIVTALWFIRSLKSFFSWLYIIQIKEYKLIRLSDYF
                     TTDKGKASIVNPLNGIKLLVLIALILSAINKISILIWPSAIIIGLIYFVESAIFLLRI
                     FKKSFNFPRITHKAALITLIGYSLIAVLAHSAISYKFNFIWLLILLGGDLLIILIALA
                     STFIIFPFSELIKKRVMKKAEKERWSLNKMLTIGVAGSYGKTSTKEFIAELLSSKFKV
                     DRTKENQNTEMAISNFILKGLNKNSEIFIAEMGAYVKGEIKLIANIVKPKIAVITGAN
                     EQHLALFGDMDNLLSAEGGKELVDSLPKNGLAIFNGNNIYAENLYKETNLSKIITYSY
                     FNPTSTVEFEPDVWAKNIKMDKTSISFDVFSKKYRDGKNFRLNLTGSFFIENVLLSIA
                     TAESLGVGMDKIYEAAKNIDDKMTGIKHIKNADGVNIIKSTYSSNPDAVKRHLEYLKE
                     AWRGSKKVFVMQPIIELGDASPKIHREIGMAIGRTCDLAIVTGRDNLKNIQEGAGIAH
                     KDKIIYSEKPEEIAEILDKNVSIGDVVFLEGRIRENIINAIQ"
     gene            complement(13980..14738)
                     /locus_tag="VI432_01565"
     CDS             complement(13980..14738)
                     /locus_tag="VI432_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4496823.1"
                     /translation="MEGWKIINGGNAKFLELGAGKPLLILHGWNSSLSSWKETAISLA
                     ENGNRVIVPDMPGFGSSDAPDRPWTTDDYTKWVLDLIRELNLKNINLAGHSFGGGLAI
                     KLAVQRPELFEKLVLISTARIRKKKSLHRSIIKVGAKIIKFFSFLPLYEKARSLLYRK
                     IVGTADYPRASGTMKDTFKNVIAEDLTPVISLINLPTIIIWGDKDRLTEIGDAHLLNK
                     SIKNSKLEIIPGIGHAPNIEHPKKLAELINKFIK"
     gene            complement(14720..15361)
                     /locus_tag="VI432_01570"
     CDS             complement(14720..15361)
                     /locus_tag="VI432_01570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEY4496824.1"
                     /translation="MGILGFYLIKPVFGFINDPEGFKTWIEILGLWAPIAMISIQILQ
                     VVIAPIPGQIIGVLSGYTFGVFWGSIYTIIGTLIGSYFLFLAARKFGRPLVEKIVKPR
                     TLSKIDSATNDGGIFALFILWLLPFIPDDIMCLGAGLTTIRIKTLMIMAFLGRLPGLI
                     ILNMAGAGFAEQNANISFIILILIAVASFLIFKYKNQLEKLSTKLIDKWKAGK"
     gene            complement(15437..15510)
                     /locus_tag="VI432_01575"
     tRNA            complement(15437..15510)
                     /locus_tag="VI432_01575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15476..15478),aa:Gly,seq:tcc)
     gene            complement(15654..15833)
                     /locus_tag="VI432_01580"
     CDS             complement(15654..15833)
                     /locus_tag="VI432_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496825.1"
                     /translation="MRKYLSIIILAVVIIAGLIIMSSVYNYFYQMKAEKQTDGLRGPM
                     GDPFTSGPTEPPPGR"
     gene            complement(15893..15967)
                     /locus_tag="VI432_01585"
     tRNA            complement(15893..15967)
                     /locus_tag="VI432_01585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15932..15934),aa:Arg,seq:gcg)
     gene            complement(16073..16522)
                     /locus_tag="VI432_01590"
     CDS             complement(16073..16522)
                     /locus_tag="VI432_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496826.1"
                     /translation="MQTLEFLVPEFEPRRKGAKWYLIFVFIIGLLILFSYFTKNPTFI
                     GLIIIGAIFIIFNNNRRPLMMPLKISDIGIALGNKMWEFGEINSFSIYQIGNRNYFIF
                     LPKGRFKNFIKVPVSKPEEIKSRLNNLITEVEYNESLIEIISRAIGL"
     gene            16563..16868
                     /locus_tag="VI432_01595"
     CDS             16563..16868
                     /locus_tag="VI432_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496827.1"
                     /translation="MKLVLIKKGDNIANLSRQMGYKLIKFDDWENEYNLVRPLSGDNY
                     PRFHLYIKEENNNWNFNLHLDQKRPSYEGSSAHSGEYGGELVEEEMERIKTFLNKSI"
     gene            complement(16861..17478)
                     /locus_tag="VI432_01600"
     CDS             complement(16861..17478)
                     /locus_tag="VI432_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4496828.1"
                     /translation="MKPRATKLKNYFLKDVNPVVRFLIISDTVLAGAGGLLGPIFALF
                     IEDYIKGSDAAVVGLAAGIFLASKSVLQIPIAHLIDKIRGEKDDFWLMFIFTFFIGLL
                     PLLYLIIDTPMELYIVEFIYGIFTAFTFPTFMAIFTRHIDKSREGTEWGVYFTLTDVT
                     GAILAALGGYIAATEGFPTLILIVVGISLVGSLLLLPIKPYIKTK"
     gene            complement(17723..18340)
                     /locus_tag="VI432_01605"
     CDS             complement(17723..18340)
                     /locus_tag="VI432_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4496829.1"
                     /translation="MNKIKIGNVEITEPRQIVYDILKYKDSGTVLDLGAGYGRHSLFL
                     AHKGFKVTAVEEDEKALESLKIRANTLGVEIATAHKDIKDFESDRKYDVVIATMVLHF
                     LNQNEVQDMLKKMQSWTAKGGINVVCAYTDQNIAKLRPYLFKVGELKEDYSGWEILEY
                     GETWGDPLTKETLEYEKSLGLLVESIQENEEIKRRHTARLIARKI"
     gene            complement(18483..18569)
                     /locus_tag="VI432_01610"
     tRNA            complement(18483..18569)
                     /locus_tag="VI432_01610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18533..18535),aa:Ser,seq:gct)
     gene            complement(18580..18828)
                     /locus_tag="VI432_01615"
     CDS             complement(18580..18828)
                     /locus_tag="VI432_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4496830.1"
                     /translation="MYNVYIIKSRKNKKYYIGYTSNQENRIKKHNSGENISTKYGAPW
                     ELIYSERFENKREAWLGEKQIKKFKGGEAFKKLIGLID"
BASE COUNT         5613 a         3339 c         3852 g         6153 t
ORIGIN      
        1 atttaatggt tccacaaaaa tttaacccac gcccgatcag ggttgatttg gatataaaag
       61 agcctatatc agaggataaa aatactttat tcagttaatt ttatgaagaa gagaatttac
      121 ttagactacg ctgcttctac ccccgtacta aaggaagttg atgcagtcat gaagtctgta
      181 gccagaggaa aaataggaaa tccaggttca atccattctt ttggtcagga ggcgatggct
      241 gttattgata aatcaagaga agatattgca gatgctatcg gcgcggattt cagcgaaata
      301 gtttttacat cgtctgctac cgaagctaac aacctggcca tcaggggagt ggtcaaaaaa
      361 gccgcttcta aaaacggtaa gaatcttaat ataatttcgt cttcaattga gcatgaatct
      421 attttagata catttaatga tctcaagaaa gatggcataa atgcgtttca tgccccggtt
      481 tctaagaatg gaattgttga tttaaagcag attaaaaaat taataaactc cgaaacagcc
      541 cttgtgtctg ttatgtatgc taataatgaa ataggcgtta tagaaccgat aagtgagata
      601 gcaaaattaa ttaaagattt tagaattgct aatcgatcta aatatccttt atttcatgta
      661 gatgctgtcc aggcttttca gttcttgaat tgtgacgttg aatttttggg ggttgatctg
      721 atgactattt cttcgcataa aatatatggt cccaaaggca tcgcggctct ctatataaaa
      781 gaattaggca agggaagcgt tatagagccg attttgactg gcggagggca ggaatttggc
      841 atgcgttccg gaacagagag ccctgcttta attgccggat ttgctaaggc ggttaagata
      901 gccgtaagag atcgcaaaag gcagttccaa ataagtaagg gcttgaggga ccatctttgg
      961 aagaagataa aagaaggatt cccgaagtca aaaataaatg gcagtttgac taataggctt
     1021 ccaaataatc taaatattta tttggcaggc aggaacgccg aagatttggt tataggattg
     1081 gacctagccg gcatagccgt ttcatccggt tcggcttgca gagcaaggtc cctagagcct
     1141 tcatatgtca tagaggcgct tggttatcca aaagaaaggg ctatgtctag cttgaggttc
     1201 agtctgggta ggcaatcgac cataagagag attgacatgg tgataaaaca atttttaaaa
     1261 ttaattaata aaaaataaaa atgaagaaaa atatcttaac attaataata gttttgtctt
     1321 ttgttggagg cattattgtg agtgattctg ttatctcaaa tacatcagcc ggaatctggc
     1381 aggatatagt tgataaagta tcgccctttt ttggcggaaa caatgtcaaa gataagccgg
     1441 cagatataga aaacgtaagc gaaccatata tatctccatc aagttatgaa gacgcggtaa
     1501 taaaggccgt ggaggatggg tcaaaaagcg ttgtctcaat tattatttcc aaagatttac
     1561 cgattataga acagtgtccc aataatcctt ttggcaacaa tccggatctt tttgattttt
     1621 tcggacgcga tttcggtttc caattttatc aaccatgtga gaagggtact aaatatcaag
     1681 aagtcggcgg aggaagcgga tttattattt ctaaagacgg tcttatcgtt acaaataaac
     1741 acgtagtgag cgacgagaag gctgactata cggttttaat taacaatggc aataagtaca
     1801 aggctgaagt tttggccagg gatcccgttc ttgatttggc cataataaaa attaatgcgc
     1861 cagaagggtt gttatatccg gcgaaacttg gcgactcgga ctcaatcaaa ctcggccaga
     1921 cgactatagc tatagggaat gcattgggcg aatttagaaa caccgtttct atcggcgttg
     1981 tttctggtct tgcgagaagc attactgcat caaacggagc ttctcttgaa aaaatagatg
     2041 acttaattca aacagatgca gctattaata agggtaactc cgggggcccg cttttaaatt
     2101 taaaaggaga agttataggt attaataccg ctattgttgc tggagcccaa aatataggat
     2161 ttgcaatacc cgttaatcaa gcaaagcgcg atattgagtc ggtaaaaaag actggcacga
     2221 taaaaactcc ttatctaggt gtaagatatt ttgtgattac tcccgaactt gctttggagc
     2281 agaaactgac ggttgaatat ggtgcgcttg ttcgtggtaa tgagtctggt ccaggagtaa
     2341 tacccgatgg tcccgccgac aaagccggca ttctgtcaga ggacattatt ttaagcgtta
     2401 atagtgaaaa acttgatggt gataaaacat tggtaagcat tattcagaag tatgatatag
     2461 gagacaaatt aaatctgggt attaaaaggg gagataatga tataacgttg gacgttgtgc
     2521 ttgaagaaag accttaggat aaaagatagt taacgggttt ataattaata gcggaaaatt
     2581 ggtataataa agttgcaaag accggattat tggtataagt tatcagtcaa attaacaact
     2641 atgaactcat ttaatcgatt cacaataaaa gctcaagaag ccctgcagaa tgctcaagac
     2701 cttgcttcag cgcagaatca tggcgagttt aaagctcttc acctgttatc agccttaata
     2761 gccgatgata gctctttggt aaggcctatt ataaccagat tgggagcaag cttggattca
     2821 atagatagga caatcaataa ggagcttaaa gagctgccta aaattttttc ttcaacatct
     2881 ttggggcagt tatatgtttc gcaggaagta atgaagatac ttgatcgcgc cgccaaggcg
     2941 gctactgcaa ataaagatga atttatatca tgtgaacacc ttcttttggg tattttagat
     3001 tctgactccg ctgccaaaaa tattttggaa acatctggca taagaagaga aagcgccatg
     3061 cgtacgctct ctgggcttcg tggaagcaca agggttactg acgaaactcc tgaaagcaaa
     3121 tttcaagttt tagaaaaata tggaattaat ttgtcagaga aggccgccga gggcaagttg
     3181 gaccctgtga taggcagaga cgaagaattg aggcgagtga ttcaaatttt atccagaagg
     3241 acgaagaata acccagtttt aattggcgag cctggagtag gaaaaacagc ggttgtagag
     3301 ggtttagctc aaagaatcgt gtcaggcgac gtaccagaaa ttttaaaaga taaagaagtt
     3361 gtctcgttgg atttggggtc gatggtcgca ggaactaaat ttagaggaga atttgaagac
     3421 agacttaagg ctttcattaa agagatacaa aattctgccg gcaggataat cttatttatt
     3481 gacgaaatac atatgatagt tggagctggt gccgctgagg gggcagttga tgcgtctaat
     3541 ctattaaaac cggctcttgc ccgaggagag ttgcacgcga tcggagcgac aacgcttaga
     3601 gaataccaaa gatatataga aaaagatgcc gccctggaaa gaaggttcca gccagtcctt
     3661 gtagaagagc cctcaataga agatagcgta actattttaa gagggcttaa acctaaatat
     3721 gaaatacatc acggccttcg tatcagtgat gaggcgatag tttctgcggt taatctttca
     3781 gcaagatata ttagtgatag attccttcca gataaagctg tagatgttat agacgaggct
     3841 tctgccgcaa gaaaaataga ggcagaaagc ctgccgatag aaatagataa gattaggaga
     3901 gagattacaa atttggaagt tgaaaaagcc gcgatcaacg gtgattcgca aaagactgac
     3961 tccaagaagc ttgccgagat taataaaaag ctaaagacgg ctaagactcg caatgaaact
     4021 ctttcttcag agtggcaggg agagaagctt atttttgaaa agcttaataa tttaagatcc
     4081 agagtcgaat ctttgagaag ggaaagagat gtggctgaac gtgaaggaca gctggaacga
     4141 gtggctcaga taatctatgg cgaattgccc gaagccgaaa aggaatatag aacttacgag
     4201 aaaaaatatt tttctatagg taaaaagaaa aacaacaaga ataaattctt aaaagaggag
     4261 gttgatgcgg aagatatcgc aaaggttata gccagatgga caggaattcc agtcagcaaa
     4321 atattagaga tagaggaaga aaaattaaag agagtggaaa cttttttgaa gaaaagagtt
     4381 gtcgggcagg aggaggccgt aaaatcagtt tcaagcgctc ttaggagggc cagagccggt
     4441 ctcgcagctc ccgatagacc gcttgcttcg tttatgtttt tgggtccgac tggcgtagga
     4501 aagaccgagc ttgccagatc tttgtctgaa tttatgttta acgatgagaa atcgttagta
     4561 agaatagaca tgtctgaata tatggaaaaa catgcggttg caaggctgat aggttctcct
     4621 ccgggctatg tcggttttga agaaggcgga caattaactg aaataatcag gcataagccc
     4681 tacagcttga ttttgtttga cgaaatagaa aaggcgcacc ctgaagtttt taatatttta
     4741 ttgcaggttt tagataatgg gcgtttgacc gacggtaagg gcagaatggt aaattttaaa
     4801 aacgctatta ttataatgac ttctaatgtg ggcagcgaat tttttaaaga gatgtctaaa
     4861 ttaggattta atatgtcttc cgatgaagcc aaagacaaag aagatgatta taaatcacga
     4921 ataaaaagct ctcttaagga taaattcaga cctgaatttt tgaataggat tgacgatata
     4981 attattttta agtctctgaa tagggaagac atagcagaga tagtggaaat tcagcttaaa
     5041 gatttaaaaa atagagtatc tgaacgaggg attaaactgg ttattgagcc taacgttaag
     5101 aaatatatta ttgagaatgg ttatgatgcg gaatacggag cgagacccat taaaaggttg
     5161 atacaaaaga ctattacaga taaattggcc gatacgataa tcggcggagg aataaaacat
     5221 ggcgctaggg ttaaaattgt ttctgaaggg tccaatatca aagttagcgt ttaggtcgtc
     5281 tgttgtggct atagcggttc tggcgttgag actaacgcct ttcggttttt tgccgacgtc
     5341 gtttttcttg attttatttt caattttata tttgcagccg aaccttaata atggaaggtt
     5401 tttaatacca ttttcggttt tattattttt tatttttttt atgcccttgg gagaaatcag
     5461 gtattacgaa ttatatattg ctgggttgtc tggtatttta ttttttatgc tttccggagt
     5521 aaaaaatgtg attttcatta atcgcaattt tatttatcgt atttctcacg gagcacttat
     5581 tttattaatt ctctctttct tttttaaata cccgccgaat ctttatattc aatttatgac
     5641 atttctatta atattttttc tatatcgcga gctttattta atgcacagat tagatcaaaa
     5701 aagaatcaat ttattatcgg cgattatggc gttgcttgtt tctgaaataa tatgggctat
     5761 ttttcttata ccgacatcgt tttcgggttt gttgtttatg tcggctgcgc ctatttttct
     5821 tgtggatgat attttaatta actatttaag aggagagagg ttgcataata tttttataag
     5881 agatttttcg ctgtttatta tattcatcat ttttgtggct ggatttatta attttgggct
     5941 tcctaggtaa ttttttgaca cactggggca gattgcctta tcatactaaa aagtcaacat
     6001 gaaaatttta tttaatctaa acaatgatat atcaaatatt aggcgggata ggagtcctgt
     6061 tgttaggggc gttaatcggt tactttgtaa ggaggttcat cttaattaag caggccgctc
     6121 ttttagagga taagttaaaa gataatgtaa ctagagccaa aaatgaggct caagatattg
     6181 ttcttgaggc taaaaacaag gccgtaagta ttttagaaga gtctaaaaaa gaagagaaag
     6241 aaagaaaatc cagtctggac aggctagagg agcgtttatt aaaaaaagaa gaggatttaa
     6301 gtcgtaaaga aaacggtttt catgaagaaa aagaaaagct tataaatgaa ttaaagcgta
     6361 ttgaagagtc cgagaaagag gtggagaaat caaaaggaga agttactaaa aagcttgccg
     6421 aattagccgg tctgacggct gaacaagcta aagagaggct gatgaaagat gttgaggatc
     6481 agaataagga agatttaact tctgcgttgc ataaaatatt caatgagagg aaagaagaga
     6541 tagagaagaa gagtcttgaa attatgacaa cagctgttca aagatatgct agatctcatg
     6601 tgtcagacat aacaacgtca gtctttaatc ttccttcaga agatctaaaa ggaaagatta
     6661 taggtcgcga gggtagaaat attagaactt tagagcgttt agccggcgtt gaattgatag
     6721 tcgacgaaac tcctgactca cttattattt cttcgtttga tccattgagg cgcgagatcg
     6781 cgagactgac attagaaaag ctgattaaag acgggcgcat acaacccgct aagatagaag
     6841 aaaaggttga agaggctaaa tccgaactgg ataaaagaat gtttgagatt ggagaggcgg
     6901 ctgcttatga gttgagcatc ttggattttc ctaaagaaat attacaactt ttgggtagac
     6961 ttaatttcag gactagtttt ggacaaaatg ttttgaccca ttctgttgaa atggctcatt
     7021 tgtccggcat gatggcatca gaattaggag ctaatgtaga ggtggcgaag cgcggcgccc
     7081 ttttgcatga tataggaaag gctattgatc atgaggtaca aggaacgcat gtcgaattag
     7141 gaaggaaaat attaaagaag tatggcgtat ctgaagaagt gatagctgca atgcagtctc
     7201 atcatgaaga atatccattt tcaactccag aatcattcat cgttaccgcc gcggatgccc
     7261 tatctgccgc aaggcctggg gctcgcaggg gaacattgga aaactatata aagagactgg
     7321 aagacttgga aagaatcgct acgagcttcc ccggagttaa aaattcttat gcgatcgctg
     7381 caggcaggga gttgagggta tttgttgttc ctgaaaaaat agatgatttt aaggccctgc
     7441 aattagctcg tgatatagca aataaaattc aatccgagct tagatatccg ggcgaaatca
     7501 aagttaatgt tataagagaa atcagagctg ttgaatacgc aaaataaggt ctatttccct
     7561 attaaagtta taattcgttg ataaatacta gcttatttgt tataataact agaaaggtcg
     7621 attaaagaga ttcaaaacat gaataaagca caattaattg agacggtgat gacatctgcg
     7681 ggccttgaga caaagaagca ggctgaatcc gcagttgagg cggtatttga caccgtaact
     7741 aaagttttgg gccgaggaga agaggtggca atcgctggtt ttggaacctt caagacctca
     7801 aagagagccg caagagctgg tattaaccct aaaacaggag agaaaattca gatagcggct
     7861 aaaactcttc ctaaatttaa agcaggaaaa cttcttaagg aagctgttca ataaattcat
     7921 taaaaagttt atacttattc taaaaccccg cctttaggcg gggttttaga ataattctat
     7981 acaaatagaa gcgttacgga gtatttttta ttttaagccc aaagttttcc taattgattt
     8041 acttacatct ataaaaatat catagactat ttttatttta ccaagactat cttccataag
     8101 gagtctgggt tcggggatat gggtgacatc ctctttttct ttacttttct cagataaatc
     8161 gctgacattg agttttaacg ccggatttat gataaaaatt ttggcttgat ttttaagctc
     8221 ataagcttgg tttatcaaac tggcgattgt gtcaaaatct ttcaggaaag agtctgtttt
     8281 gaggaaacct aatttttcaa gcttttctac atctttggaa attcgttcag ttttttcttc
     8341 agccgtttta ataacgtttt cgttgtagaa gagcaataaa aactccaaga gttgagaaat
     8401 cctcgggtta taagactttt ctcggtaggt tattatttct tcagctaagg cgcgtccatc
     8461 ctccagagtg cctattctat tgagttcttt catcttaata ttgtagaatt gagcgaaggt
     8521 tgaaagctct tcagcaaaaa cgctttttag ttcttgctct ctgctcttcg cggagaattg
     8581 aggtagggtt tccatgtctt tttttaatgc taccacttct tgtatagata aatttattgc
     8641 atcttttaca acggatttta ttattagcac tttcgcttca ttgtcctctg tcgagttgct
     8701 tataggatta agatcgctgt ctccggctag cgataataac tcatcatagc ttgaatcaat
     8761 tctatttcga gcttctttta gcccttcttc tgttgcgtgt gtaatggacg gcgcaagagc
     8821 aaaggctaga attgccataa tgcccatttt aattgcttta ttcatggttt ttattttaat
     8881 gatccggtct tggtaagagc ttcgctcaga gccattgtcg ttttatttgc agaggacgca
     8941 aaatattctg cttctagtaa ttttatgttg atatctttgg ttagattatc cgcctcgatt
     9001 gctagatctt cgttttcaag cgcagataac tccggtaaga aagcctcttt aatgtttggg
     9061 gttgttgtta taactgttat ggctattata gatagcgcaa ttgccatact aaaacttagt
     9121 gattgatgta taagcctggg gattgtaccc atattcttaa tattgaatcc aagatccata
     9181 agaggaggct tggcggcgat cgcgtggatt gcgtctttgg tttttaaggc gaattcggtt
     9241 ctcggctgta tggcgcggag catgtttaat ctagatacta aatcattatc cagccccgcc
     9301 tctttattta atagtattga tctagaaata agcgcgtaat cttctgacgg tttgatctta
     9361 tttattttgc ttaatctttt tattaaatct tggtcggtca ttttaattct tgtttaatct
     9421 ttcttagcgc tctatgttgt ataagtttta ctgcccctat tgtttttcct acagcgtctg
     9481 cgacctcttc gttagacatc tcatctatga agcgcattat gacaacgtct tgttctatcg
     9541 gtttaagttt tttaatcgca tccataaccg tttgaatttc aatagcgttg tctaccgatt
     9601 tatctaaatg atcgtcttcg ctgggaataa tttctagcga gtaatctagc gttatttcgg
     9661 ttttccttcc attagaactt cggtagtggt ctattatggt gtttctggct atcttgtata
     9721 accagctgcc gaaagggaaa ccttgatctt ggtattctcc tattctttcc caagccttaa
     9781 gaaatgccag gtgggtgaga tcttcagctt cctcacgatg gcttacttta agtaggacga
     9841 acctatatat cctttcgaga tagtggtcat aaagcaaacc gaaagcgtta gcctccccct
     9901 ccctagcttt tcttatgatt ttcttctcgt cctctagcat ttaaaacgtt ttttggcttt
     9961 tatagttact aaaaccccga taaatactgg attatcgggc ttaaagttaa gatcgtggct
    10021 atcataacaa caatattaag aaaggtaaaa ttagctataa tcctcttttt attcttctta
    10081 aagactcaac aaaataattt aaccaatatt tggaatttat cggtaaatct tgcgcgggca
    10141 cgtagtcttc gctgtatccg ccaaaaccgg ttttaaagag ggttagtcct ttccaggggt
    10201 gattgttttt attattgggc gctattcccc atagattata ttttttacag tttcgccttc
    10261 gagcctcctg aataattttc cattgcaata aatgtgatgc tggaattttg gggaaatttt
    10321 gatttgatgc accatgatga taatatgctc cattttcatc aaatattata aatgccccgg
    10381 atagatcctg gttcttatat ttagcgatta atatttcaga tttattgttt tgcgagaaaa
    10441 ctttaaattc attttctata tatttatcgg agaagggagt aaaaccctgt ctttctgagg
    10501 ttgtttcgta tattttctca aacctttgaa cggcattttt ttctgacata agtatctcaa
    10561 tttggactcc ttcctgctcg gcttttttta taagctggcg cgtagtcttt ctcatgtctt
    10621 ttaaaattgc ctcctcgctt ttattaatat caataaccca cgctttttcg gggtgcatat
    10681 ggataggcgc gttaataaag ccgagatttt taaatatctt tctattattt ggcgtgtcgg
    10741 tcaggaggga gcttattcta ataaaagaac acccttcatt attagcgatc tcttttaact
    10801 tatcggttag ttttgataaa acttcatcta ttaaattttc gactttagat attttaataa
    10861 ttggcccatg tggacagaaa ataaatgatc cgcgtctggc atcggtttta ataattagcg
    10921 cgaccgaaat taattcctcg ttatcaaatg ctccaaggcg atatattttg cctttcatct
    10981 cttcattaaa ttgaccccat tcccatgaat gtaagaatgt gtttggttta acctctttga
    11041 taaaattatc ccaaacaaat ctgtctttta tcgttttaat ttttatattc atattgttgg
    11101 agaataaaat cagctattct attagcgtct tcttctagaa tattcggtcc agtcggcaga
    11161 ttaagagatt tttgcgcagc aagctcggct tttgggcacg acccaggaat atatcccgcc
    11221 cttttctgat ctactccttg cggagctatt ggtggcctgt accagtcacc aaacaaaata
    11281 ttttcttttt ttgcttttgc aattattgtt tcggcttgat ctgtctgtat ggtgtatcgt
    11341 aaatatacgc tgtttccttc tcttgtgggg aggatcaccc cgttattatt aagtttttct
    11401 aatctttcat cgtagatcct cgctattctt ttgcgatgct cattaaatct tttaattttc
    11461 ctaatttggt tcaaggccag ctctgcgatt gcattaggca ttttatacga aataaattca
    11521 ggtttgccgc ccgttttttc taatttatat actgccattg aggttagatg gagttttttg
    11581 gaggcttcaa taataatctt gccgattttt aatatgccgt agagcttgat cgccatatta
    11641 aatattatcg gatggagaag ctgttgctta atccatgttt ttttaggatt attaagatga
    11701 tttagttcct tatttatttt ttctgataaa tctgagttgt tggttgctat cgctccgccg
    11761 taaacagatg atattatttt atctcttccg aaactgaaaa agcttgcgtc tccaaatgaa
    11821 cctgttgcgg cgtttttgta ttcagctccc agagagtgtg cgcaatcttc tataatttta
    11881 agattatgct tttcggcgat ttttttaatg ccgtctatgt cggccggcag accgaaagtg
    11941 tgctggataa ttattgcctt agagtcctta gttatttttt tttctaaatc atccgttgac
    12001 atatttaggg tctgtggatt tatgtccacg tatattggtt tcgctccagt ccattttatg
    12061 gaatttgcaa cggcgacaca ggtataagcc tgcaataata tttcatcccc attttttatt
    12121 ccaagcgctt ttagaatcgt gtatagagct gcccgcccgc tatttgtgat atagataaaa
    12181 tcaactttta ttattttttt cagctctgat ataagctgat tgatcgcatc tcctttaagc
    12241 cattttcgtg gcgagaataa aagcttaata gcaagtttta tatcgtcact ctctgcattg
    12301 ggggaaagcg atattaatat cggtttatat ttattgaata gcattaatta tgttttctct
    12361 gattctccct tctaaaaaga ctacatcgcc aatggagacg tttttgtcta aaatttcggc
    12421 tatctcctcg ggtttttcag agtatattat tttatcttta tgcgctatgc cggcgccttc
    12481 ttggatgttt tttagattat ctctgccggt tactatagcc agatcgcatg ttcttcctat
    12541 tgccattcct atttctctgt gtattttagg agatgcgtcg ccgagttcta taattggctg
    12601 catgacaaaa acttttttgg atccgcgcca agcttctttt agatactcca aatgtctttt
    12661 aactgcgtct ggattggatg aataggtgga ttttataatg ttaactccat cagcgttttt
    12721 tatgtgtttg atgcccgtca ttttgtcgtc gatatttttc gccgcctcat atattttatc
    12781 catgccgacg cccaaagatt cagccgttgc tatagagaga agaacgtttt ctataaagaa
    12841 tgagccggtc aggttaagtc tgaaattttt gccgtctcta tatttttttg agaagacatc
    12901 gaaagaaata gatgttttat ccatttttat atttttagcc catacatccg gttcaaattc
    12961 gacggttgat gtcggattaa aataactata tgttattatt tttgaaagat tagtttcttt
    13021 atataaattt tccgcataaa tattgtttcc gttgaatatt gcaaggccgt ttttaggcag
    13081 agaatcgacc aactctttgc ctccctcggc gctcagcagg ttgtccatgt caccgaaaag
    13141 ggcaagatgt tgttcgttag cgcccgttat aacggctatt ttgggtttta cgatatttgc
    13201 tattagctta atctctcctt tgacgtatgc tcccatttca gcaataaaaa tttccgaatt
    13261 tttattcaaa cctttcaata taaaatttga aatcgccatt tctgtatttt ggttttcttt
    13321 cgtccgatca accttaaatt ttgatgatag aagttcagca ataaattctt tagttgaggt
    13381 cttgccatag ctgcctgcaa cgcctattgt gagcatttta tttaaagacc atctttcttt
    13441 ttcagccttt ttcataaccc tctttttaat caattctgaa aaagggaata ttatgaaagt
    13501 cgacgctaga gcaattagaa ttattaacaa atctcctcct aaaagaatta aaagccagat
    13561 gaaattaaat ttatagctta ttgcagaatg tgctaatact gcgattaaag aatagccgat
    13621 taaagttata agggcggcct tgtgcgttat gcgtgggaaa ttaaaagatt ttttaaatat
    13681 acgcagtaag aatatggcag actcgacaaa atatataagc ccaattatta ttgctgacgg
    13741 ccagatcagt atgcttattt tatttatcgc cgacaggatt aatgcgatta atactaaaag
    13801 tttaattccg tttaacggat taacaataga agccttgccc ttgtctgttg tgaaatagtc
    13861 agacagtctt atcagtttgt attctttgat ttgaattatg tagagccaag aaaaaaacga
    13921 ttttagagat cttataaacc atagcgctgt aacgattgct atcaatattt gttccacgat
    13981 cattttataa atttatttat taattcagct aattttttcg ggtgttcaat attcggcgca
    14041 tgtccgatcc ccgggataat ctctagtttg gagtttttga tacttttgtt taatagatgc
    14101 gcatcgccta tttctgttag tctgtcttta tctccccata ttattatggt gggcaggttg
    14161 attaaagaaa tgacaggggt taggtcttcc gctattacgt ttttgaaagt atctttcatt
    14221 gttccgcttg ctcgtggata gtcagccgtg ccgacgattt ttctataaag taagcttctt
    14281 gctttttcat agagagggag gaaagaaaaa aactttatta ttttagctcc gactttgatt
    14341 atgctgcgat gcagagattt cttctttcta atccttgctg ttgaaataag aactaatttt
    14401 tcaaaaagct ctggtctctg cacagccagt tttattgcaa gacctccgcc aaacgaatga
    14461 ccggccaaat tgatattttt aagatttaat tcgcgtatca aatctaatac ccattttgta
    14521 taatcatccg ttgtccatgg cctgtcgggc gcgtcggacg atccgaatcc cggcatatcg
    14581 ggcacaataa ctcgatttcc attttctgct aaagatattg ccgtttcctt ccaagatgag
    14641 agcgagctgt tccaaccgtg gagaatcaaa agaggttttc ctgcccctaa ttctaaaaat
    14701 ttggcattcc caccgtttat tattttccag ccttccattt atctattagt ttggttgata
    14761 atttttccag ttgattttta tatttgaata tcaggaatga agcgactgct atcagtatta
    14821 gaattataaa agaaatattg gcattctgct cggcaaaacc agccccggcc atattgagga
    14881 ttattaaccc aggcaatctg cccaagaaag ccataatcat aagggttttg attctaatgg
    14941 tggttaaccc tgctccaaga cacattatgt cgtctggaat aaaagggagg agccataaaa
    15001 taaataatgc aaatattccg ccgtcattgg ttgcggaatc tatcttggat aaagttctgg
    15061 gcttaactat tttttcaacc aatggtctgc cgaatttgcg cgcggcaaga aataaaaaat
    15121 aagatcctat taaggtgccg ataattgtat aaatagaccc ccaaaatact ccgaaggtgt
    15181 acccgcttaa aactccgata atctgtcctg gaattggcgc gataacaacc tgtaatatct
    15241 gaatggaaat catagcgata ggagcccata agcccaaaat ttctatccac gttttaaaac
    15301 cctcaggatc atttataaat ccgaaaacag gttttatgag ataaaatcct aatataccca
    15361 acgcgacaat tgcaaagagc agtagatatt tattcttata tagagtggat aaattaatgt
    15421 ttttatagat attttatgga gcgggatacc ggaatcgaac cggcgcccat accttggaag
    15481 ggtatcgtac taccattata ctaatcccgc ttctttaaaa ctcagatgcg caaatccgca
    15541 gaatcaagtc tatgagcttt atatagaccg ccgcctttta gctgttagtc atattagact
    15601 taaatttata agccatcaag tttattttag agcataaaac acaggcttat aggttatctg
    15661 cctggcggcg gttcggtcgg tccgctggtg aacggatcgc ccataggtcc gcgcagaccg
    15721 tctgtctgtt tttctgcctt catctgatag aagtaattat aaacgctgct cattatgatg
    15781 aggccggcga ttataactac agcaagaatt attatactaa gatattttct catgtgtttt
    15841 tgcgaggaat cgaatttaga ttataagatc tgccaatgac aaagttttgt cttggtgctc
    15901 tcgtgaggag tcgaacctca atttcctcct tcgcagggag atgtcctatc cattgaacga
    15961 cgagagcatt taaatgggca ggcaatctcg cggcgctact tgcccgccta agaatagcgc
    16021 gcttgcttac agggtccgtt ttcgtctttt gcggaaacaa ctgaaacaag ccctatagcc
    16081 ctatcgctct gcttataatc tctattaaag attcattata ttctacttct gtaattaaat
    16141 tattcaatct tgatttaatt tcttcgggtt ttgatacagg aaccttaata aagtttttaa
    16201 atctaccttt gggaaggaat ataaaataat ttctgtttcc tatttgatag attgagaaag
    16261 agttaatttc tccgaattcc cacatcttat ttcccaaggc aattccgata tctgagattt
    16321 taagaggcat catcagaggc ctcctattgt tgttgaaaat aatgaagata gccccaatga
    16381 tgataaggcc gataaatgtc gggtttttag taaaatatga gaagaggata agcagtccta
    16441 ttataaagac aaaaattaaa taccattttg ctcctttgcg tcttggttca aattctggga
    16501 ccagaaattc taaggtctgc attatttgta tattagcatc aagtaatgaa taattcatta
    16561 gtatgaaact cgtcctaatt aaaaaaggag ataatatagc gaacctatca agacaaatgg
    16621 gctataagct tattaaattt gacgactggg aaaacgaata taatttagtt cgtcctttat
    16681 ccggcgacaa ttatccgaga tttcatttat atataaagga ggagaataat aattggaatt
    16741 ttaatctgca tttagatcaa aagcgcccgt catatgaggg cagttctgct catagcggag
    16801 aatatggcgg agagttggtt gaggaagaga tggaaagaat aaaaactttt ctgaacaaat
    16861 ctatttagtt tttatataag gcttgattgg caaaaggagc agagagccaa ctagtgatat
    16921 gccgacaact attaatatta gcgttgggaa accttcggtt gccgcgatat atcctccgag
    16981 cgccgcgagt atcgcgcctg tcacatcggt cagagtaaaa tagactcccc attcggttcc
    17041 ttctctgctc ttgtctatat gccttgtaaa tatcgccata aatgtaggga atgtgaaggc
    17101 ggtaaatatt ccatatatga attcaactat ataaagttcc attggcgtat caataataag
    17161 ataaagaaga gggagaaggc ctataaaaaa tgtgaaaatg aacattaacc agaaatcatc
    17221 cttttctccg cggattttat ctatcaagtg ggctatgggt atttgaagga cgcttttaga
    17281 cgctagaaat attccggccg caagaccgac taccgccgca tcgcttcctt ttatataatc
    17341 ttcaataaaa agagcgaaaa ttggacccaa aagtccgccc gctccagcca gaactgtgtc
    17401 agagattatt aaaaatctga caacaggatt aacgtctttt aaaaaatagt tttttaattt
    17461 agtcgcgcgc ggcttcattg atgtttttaa taagataaag ataccataaa aatatattat
    17521 ttggttggat agtcagcgtt atgatattct tgattgtaaa ccgtctctgt cgcgcctgcg
    17581 gcgcgacccc atttcggctt ccaattccga ttttcaattt tggcgggata ctcgggttaa
    17641 gtacccactg gggtcaatac cagtgaggat tttctgggtc gtatggtggg cactagctga
    17701 ggtactcggg tcaacaacga atctatattt tcctcgctat tagcctcgct gtatgccttc
    17761 tttttatttc ttcgttctct tgaatgcttt ccactaaaag ccccagtgat ttctcatact
    17821 caagagtttc tttggttaac ggatctcccc aagtctctcc gtattctaga atttcccaac
    17881 cagaataatc ttctttaagt tctcccactt taaacaagta tggtctaagc ttagctatat
    17941 tctgatccgt atacgcgcag acgacattga ttcctccttt tgctgtccag ctctgcattt
    18001 tctttagcat atcttgtact tcattctggt ttaagaaatg caggaccatt gtggctataa
    18061 ccacatcata tttcctgtcg gattcaaagt cttttatgtc cttgtgagct gtagctattt
    18121 caactcctaa tgtgttggct cgtatcttaa gagattccag cgctttctca tcttcctcga
    18181 ctgcggtaac tttgaaacct ttgtgagcca agaataaaga gtgacggcca tagcctgcgc
    18241 ccagatcaag aacagtaccg gaatctttat acttcaaaat atcgtataca atttgacgtg
    18301 gctcagttat ctcaacattt ccaatcttta ttttgttcat taaatttaaa tattcagata
    18361 taaccgactc tgatagcact cgggtctgcc gataaaaaat tttctgattg caaagaagca
    18421 agtatacgtg ccacattttt ctatcatatc acgcaatggc ttgcactgag cttgccgaag
    18481 tgcggagagg gagggattcg aaccctcgag gggattgctc ccctaacatc ttagcaggat
    18541 gctgctttca gccactcagc cacctctcct tatttagatt taatctatca gacctataag
    18601 ctttttaaaa gcttcaccgc ccttaaattt tttaatttgt ttttcgccta accaagcttc
    18661 tcgtttgttt tcaaatcttt cagaataaat tagttcccag ggcgccccat atttagttga
    18721 tatgttttct ccagagttat gctttttaat tcgattttct tgattagaag tatatcctat
    18781 ataatatttt ttatttttac ggcttttgat tatatagacg ttatacattt atacttagcc
    18841 acccaccgcc tttggcgggg tcccgctgta agcggtgact ctccgaaact tattaaagaa
    18901 tacttttaaa tcttttattt atcaaacatt tttagccttg ttatataagt gtttatg
//
LOCUS       DATDNZ010000043         5132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2213830, whole genome shotgun sequence.
ACCESSION   DATDNZ010000043 DATDNZ010000000
VERSION     DATDNZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213830"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VI432_01620"
     CDS             complement(<1..399)
                     /locus_tag="VI432_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4496831.1"
                     /translation="MKILRDQTLTWVNLDNPSADDIAWLQKEFNLHPVVIKDLNLPMR
                     HPKIDVFGNYIFAAFQIPIEKDGAGTALRIEELDLIISENWLISSHTSRLPFVNDFFA
                     KCMGSQETKSEYFRSGPAAVIHGLISGLFES"
     gene            1000..1731
                     /locus_tag="VI432_01625"
     CDS             1000..1731
                     /locus_tag="VI432_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496832.1"
                     /translation="MPTITNRQGNILKTVIESYIGGAHPISSDFLNKSGYFRLSTATL
                     RAEMATLERSGFLMQPHTSSGRIPTELAYRFFVEKNSSTWNLSNAIRDKLSQILKIWK
                     QDKVSAVERAAEELADFFQGVSLVNLNLNNNPLFFQRLNYFRSQPEFYSHQVMDEMLN
                     VLELLQSRRNLITESVDIDKTQVLIGKDIRLGKFKVLSLIIKPWHSRYGYSSGFIAAV
                     GPMRMHYERAIPAVDFLGSLISEEK"
     gene            1736..2230
                     /gene="grpE"
                     /locus_tag="VI432_01630"
     CDS             1736..2230
                     /gene="grpE"
                     /locus_tag="VI432_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY4496833.1"
                     /translation="MEDNLENSQKHLDSSSQSGAGGKQAAQEYESKWKKVLADFENYK
                     KDEAKKFSVFAKFSKIEALRDLLPVFDMLERAVHEIPQDIKSYKWVDGISLIYEEFLS
                     ILAKNGFKKIETNSKSFDPKYHEAVGRIDGVGDDVVEDIRSGFVTEDDFVIRAVQVKI
                     GKKK"
     gene            2285..4189
                     /gene="dnaK"
                     /locus_tag="VI432_01635"
     CDS             2285..4189
                     /gene="dnaK"
                     /locus_tag="VI432_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY4496834.1"
                     /translation="MSKILGIDLGTTNSAMAIMEGGEPKIIENSEGARTTPSMVAANK
                     SGERLAGILAKRQAVTNAENTIFSVKRLIGRRFLDQEVQRDKQWLPYTIREASNGGVE
                     VKMVDKWYAPQEISAMVLSKLKTNAEARLGIKITDAVITVPAYFDDSQRKATKEAGEI
                     AGFNVRRIINEPTAAALAYGLEKKKNEKIVVYDFGGGTFDVSVLEISTDTVEVKATGG
                     DTHLGGDDFDKRLIEWMVDEFKKDQGIDLSRDKLALQRLKEAAERAKHELSSTHETEI
                     NIPFITSDAEGPKHLLLKLSRAKLEELVNDHIEKAAQIVAKVVGEAKFALSDINEVVM
                     VGGQTRMPAIQDSVRKLFGKEPHTGINPDEVVAIGAAAQGGIMEGEVRDVLLLDVTPL
                     SLGIETLGNVMTPIIEKNTTIPVSKSQVFSTAADNQPSVEIHVLQGERVLASDNKTLA
                     RFILDGIPPSPRGIPQVEVSFDIDANGILNVAAKDKATSKVQTVRIEASTGLSKEEIE
                     RMKKEAELHAEDDRRKKEMVETINTADALIYSVERTLRENTDKISLDIRSSVEDKLKE
                     LKEAKSKDDIAALRRAIEDVNREAQKIGSSLYEQNKNSSPSDANSDSQGQNKEDIADG
                     EVETDETAPD"
     gene            4214..>5132
                     /locus_tag="VI432_01640"
     CDS             4214..>5132
                     /locus_tag="VI432_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496835.1"
                     /translation="MLEAITKLKIVLPNFFILAFFILCMTGVGWFFGRALNGTSASIE
                     EFLGLNFALNDILIGTVLILLSAMWFSIAVMLDDSFRLPAVASLVALFLMSLFLFPAP
                     SIYMLVILPAWALLLLERLRMRHVLKDLMPWKPSWALHAGLPKFFLAINIIFTAALAV
                     SPTALFFLRQPLPRSFIDQSLLFLSAPIQQYTGIDVNSTVDQTLNHLNEGKLTDPQLL
                     TIARTEYSKKLNLPLTGKEKIKDVIFNVIDRQLNQFAKQSINIFNTGHINLFLLGFVA
                     SVFFTFQFFTWPANIISNIMTRILLFLLEA"
BASE COUNT         1573 a          952 c         1101 g         1506 t
ORIGIN      
        1 gctctcaaaa agtccagaaa ttaatccatg tattacagct gccgggccgg atctgaaata
       61 ttcacttttg gtctcttgag aacccatgca tttagcgaaa aaatcattta caaatgggag
      121 ccggctggtg tgactggaaa taagccagtt ttcgctgata atgaggtcta gttcttctat
      181 tcttaaggct gtgcccgcgc catctttttc tatgggtatt tgaaatgcag cgaaaatata
      241 atttccaaaa acatctattt ttggatgacg cattggtaga tttaagtctt ttataacaac
      301 cggatgcaag ttgaattctt tttgtagcca ggctatatcg tctgcgcttg gattatcaag
      361 gttcacccac gtgagtgttt ggtcgcgtaa tattttcatc ccgttagaag cagatcgcga
      421 tcaatgatct caatctgcaa aattatttgt atggatacta tatacgacat agatattttt
      481 accccgttag aaagctcgca tcacaaacac atcaattata atattcccta taaaagtttc
      541 aaaaataact ttctaacggg gtttattgct aatcctttgc gatcgcggct tctaacggga
      601 ttcatctcgt tagagacagg aagcgcttta cgcaaaagac taaagcgctt ccgtaatatc
      661 gcgcataaat cccgcttaaa gttttaatca ttttatagaa tacaaattac ttgctattcc
      721 tattaggtcg tctctaacgg gattcataaa taaaatataa tttttatcgt aattcattat
      781 agcatttaag tattgtattg acaggtagcg gtctttttat ataatgcttt tagcactcgt
      841 gatattagag tgccaaaata atgaaatcat gccaggtagt gatccagctc caatatacgc
      901 taattcccgg gctcattatt ttggctcgtg tttataaaaa agactaattt ttaacatccc
      961 gctttaataa ataattatgg ggcgggacta ctacctagca tgcctacaat aaccaaccga
     1021 caaggaaata ttctcaagac tgttattgag agttatatcg gtggcgcaca cccaataagc
     1081 tccgattttt taaacaagag cgggtatttc agattgagta cagcgacatt acgtgcagaa
     1141 atggccacac ttgagagatc cggattcttg atgcagccgc acacatcatc tgggcggata
     1201 cctaccgaat tagcctatcg ttttttcgtg gaaaaaaatt ccagtacctg gaatctttcg
     1261 aatgcaattc gggataagct aagtcaaatt ttaaaaatat ggaagcagga taaagtaagc
     1321 gccgttgaaa gagcggcaga ggaactggcc gatttttttc aaggcgttag cctggttaat
     1381 cttaatctta ataataatcc tttgtttttc cagcgattaa actattttag atcgcaacca
     1441 gagttttatt cgcatcaggt tatggatgaa atgctgaatg ttttagaatt attgcaaagc
     1501 agaaggaatt taatcacgga atcagtagac atcgataaaa cccaagtatt aataggcaaa
     1561 gatattcgtt tgggcaaatt taaggtttta agcttaataa taaaaccttg gcatagcaga
     1621 tatggctatt cgtctgggtt tattgccgcc gtaggtccaa tgcgcatgca ttatgaaaga
     1681 gcaattccag ccgtggattt tttgggttca ttgatttcag aagaaaaata gatttatgga
     1741 agataatctt gaaaatagcc aaaaacattt agatagctcc agtcagtcgg gagccggcgg
     1801 caaacaggcc gctcaagaat atgagagtaa gtggaaaaag gttttagccg acttcgaaaa
     1861 ttataagaaa gatgaagcta aaaaatttag tgtctttgcc aagttcagta aaatagaggc
     1921 cctgcgagat ttattgccgg tttttgatat gcttgaacgc gctgttcatg aaatcccgca
     1981 ggatataaaa agttataaat gggttgatgg aataagcctt atttatgaag agtttttgag
     2041 catattggct aaaaacggtt ttaaaaaaat agagactaac agtaaatctt tcgatcctaa
     2101 ataccatgaa gcagtcggca gaatagacgg cgtgggtgat gacgtcgttg aagatattcg
     2161 ttcggggttt gttactgaag acgatttcgt tatacgcgcc gtgcaagtta agatagggaa
     2221 aaagaagtaa taaattgaaa ttaagaaatc gatgtaaaca ttttttaact tttaaggaag
     2281 ttatatgagt aaaattcttg gtatcgatct tggcactacc aattctgcta tggccattat
     2341 ggagggtgga gagccaaaaa tcattgagaa ttctgaaggg gcgcgcacca cgccttcaat
     2401 ggtggctgca aacaaatcag gtgagcgttt ggctggaatc cttgctaaac gtcaggcggt
     2461 tacgaacgca gaaaatacca ttttttccgt aaaacgtctt atcggtcgaa gatttttgga
     2521 tcaggaagta caacgcgata agcaatggtt gccttatact atacgtgaag cttcaaacgg
     2581 cggggtggaa gtgaaaatgg tagataaatg gtatgcgcca caggaaattt cagccatggt
     2641 tttatctaag ctaaaaacta atgccgaagc tcgtctcggg attaaaatta ctgatgccgt
     2701 aattacggtc ccggcttatt tcgacgattc acagagaaaa gcaactaaag aagcaggcga
     2761 aattgccggc ttcaatgtaa ggcgaataat taatgagccc acggcggccg ccttggctta
     2821 tggccttgag aagaaaaaaa atgaaaagat agtcgtttat gatttcggcg gcgggacatt
     2881 tgatgtttct gttttggaaa tatctaccga tacagttgaa gtgaaagcta ctggaggcga
     2941 tacccatctt ggcggcgatg attttgataa gcgtttaatt gaatggatgg tagatgagtt
     3001 taagaaggac cagggtatag atctttctcg cgataagtta gctctgcaga gattaaagga
     3061 agccgcagaa cgtgccaagc acgaattatc tagcactcat gaaacagaaa taaatatccc
     3121 tttcataact tccgatgccg aaggcccgaa acatcttttg cttaaactat cgcgagccaa
     3181 gttggaggaa ttagttaatg atcatataga gaaagccgca caaatagttg ctaaggttgt
     3241 tggggaagca aaatttgcac tatccgatat taacgaagtt gtgatggtcg gcgggcaaac
     3301 cagaatgcca gcaatacagg attcggtgcg aaaacttttc ggaaaagaac cgcacaccgg
     3361 cattaatccc gacgaagttg tagctattgg agcagcggcc caaggtggta ttatggaagg
     3421 agaagtccgc gatgttcttt tgttggacgt cacgccactt tcacttggga ttgaaacatt
     3481 gggtaatgtc atgactccca ttatagaaaa aaataccacc atccccgttt caaaatccca
     3541 agttttttct accgctgctg ataatcaacc atcagtagaa atccacgttt tacagggaga
     3601 gcgtgttttg gcctctgaca ataaaactct agcccgattc attcttgatg gtattccgcc
     3661 ctccccacgc gggatcccgc aagtcgaagt atcgtttgat atagacgcca acggcatttt
     3721 gaacgtcgcg gctaaagata aggctacgag taaagtccaa acagtacgta ttgaagcttc
     3781 cactggatta tccaaagaag agatagaaag aatgaaaaaa gaagccgaat tacacgctga
     3841 ggatgatagg aggaaaaaag aaatggttga aacgattaat acagccgacg cgcttattta
     3901 ttctgtagaa cgcaccttgc gtgaaaatac tgacaaaatc tctctagata tccgttcttc
     3961 tgtagaagat aagttaaaag aacttaaaga agctaagagt aaagatgata tagcggcttt
     4021 gagaagggcg attgaagatg ttaaccgaga agcacagaag attggttcaa gtttgtacga
     4081 acagaataaa aattcttcgc cgtcagatgc caattcagat tctcaaggcc aaaataaaga
     4141 ggatatagcc gatggcgagg ttgaaacaga tgagactgcg cccgattaaa atcaattatt
     4201 ctctaaaaag catatgctag aagcgataac caagctaaaa attgtcttgc caaacttttt
     4261 tatccttgcg ttttttatac tgtgcatgac gggtgtaggt tggtttttcg gtcgggctct
     4321 taacggtact tcagcgagca tagaagagtt tttgggttta aattttgctc tgaatgatat
     4381 tttgatcgga acagtgttga ttttgctatc ggccatgtgg ttttctatag ccgtgatgtt
     4441 ggatgacagt tttcgtctcc cagctgtagc ttctttggtc gcattatttc tgatgtcgtt
     4501 gtttcttttt ccagcacctt caatatacat gttggttata ttacccgctt gggctcttct
     4561 tcttttagaa aggcttagaa tgcggcatgt tttgaaagat ctaatgccat ggaagccttc
     4621 ctgggccttg catgccggtt tgccaaaatt cttccttgct ataaatatta tctttaccgc
     4681 cgcgcttgcc gtgtcgccta cagccttatt ttttctgcgt caaccattac ccagatcgtt
     4741 tatagaccaa agcctacttt ttttgagcgc tccaatacag caatataccg gtattgacgt
     4801 taattcaacc gtggaccaga cgcttaatca tttaaacgaa ggcaagctta ccgacccgca
     4861 acttttgact atagcgcgta ctgaatatag taagaagctt aatcttccgt tgaccggtaa
     4921 ggaaaaaata aaagatgtta tattcaatgt tatagatcgc cagcttaatc aatttgccaa
     4981 acaatcaatc aatattttta ataccgggca tataaatttg tttttattgg gtttcgttgc
     5041 tagcgttttt ttcacctttc aattttttac ctggccggca aacatcattt caaatatcat
     5101 gacgcgtata ttactttttt tgcttgaagc cc
//
LOCUS       DATDNZ010000044         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3501192, whole genome shotgun sequence.
ACCESSION   DATDNZ010000044 DATDNZ010000000
VERSION     DATDNZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501192"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1716)
                     /gene="cysS"
                     /locus_tag="VI432_01645"
     CDS             complement(322..1716)
                     /gene="cysS"
                     /locus_tag="VI432_01645"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4496836.1"
                     /translation="MKFSFYNTLTRQKEELKPLKPKKIDIFVCGPTVYDLAHIGHGRV
                     FVIFDAVVKTLRHSGYNVRYLQNITDVDDKIIARAELQGKTPKALAHKFEHEYLKDMK
                     ALNITSPDNYARASDHIDEILSQIKRLLKKDCAYITPNGVYFSVKNFHDYGKLSHQKL
                     DKMEETERIEHDPDKKHKQDFALWKKAKAGEPFWPSPWGKGRPGWHIEDTAIAEKYFG
                     QQYDMHGGALDLIFPHHECEIAQMETISNKKPYVRMWLHAGFLNVRGEKMSKSLGNII
                     SIRDLLKDYSAQAFRMLIFQAHYRSPIDYDEDLMKQAQAAQERLTDFYNRLKTIKRFG
                     PENDRIKSIIFTSNKNFWEKLEDDFNTPRAIAIIFSLMKTLNPLINNFAISKREAKAA
                     KEFLKNADGIMHILGPKQTSSIPQEVKNLINERELMRKERRWTEADQSRKELAQIGWT
                     IEDTPEGPKPKRIE"
     gene            complement(1869..>2391)
                     /locus_tag="VI432_01650"
     CDS             complement(1869..>2391)
                     /locus_tag="VI432_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4496837.1"
                     /translation="ESVNTELDNIEGQIAVIEDEIFRGREQNMVIIISQTRRKIIDFL
                     RTLEPMQGLFKELLISGPSLLGKEARPYLEYLSESHAKLVNVLITQKETIAALEETNQ
                     SLLSTKTNATLRLLTVFTAIMLAPNTIAQIFATNISNIPFSHSANGFLIVTSVIFISG
                     LFTFLFFKFKRWI"
BASE COUNT          615 a          440 c          511 g          825 t
ORIGIN      
        1 gggcatttct aaagttatac attataattt tatataaata tatcaaattg tttttggtaa
       61 attagggctg gggagtggct gcaacaactg cataccgagc gcggtgaggt attacctttt
      121 aatagtgctc ttttgcttcg gctgagtcta aggattacct taattattct aagcttatcg
      181 tttaaatgtc ggtagagcaa tagctgcaac gtggagaaaa tgtggacgat tgtaaggagt
      241 cagcggcatg cctggctcgg caagatattt cgatatgccg tacagctgtt atggcaaaaa
      301 ggctgtttgg tgttcaccag atcactcgat ccgtttcggt ttcggccctt caggagtatc
      361 ttctatcgtc cagcctattt gagcaagctc tttccttgat tgatcggctt cagtccagcg
      421 tctttctttg cgcataagtt cccgttcatt gataaggttt ttaacttctt ggggtattga
      481 tgaggtctgt tttggcccaa ggatatgcat gataccatcc gcatttttca agaattcttt
      541 tgcggccttg gcttcacgtt ttgatatagc aaagttgttt ataagggggt ttagcgtttt
      601 cataagcgaa aaaatgatcg ctatggcgcg tggtgtgtta aaatcatctt ctaatttttc
      661 ccagaaattt ttatttgaag taaatataat gctttttatg cggtcgtttt ccggcccaaa
      721 cctttttatt gttttgaggc ggttataaaa atccgtcagg cgttcctgcg ccgcttgcgc
      781 ctgtttcatc agatcttcgt cgtaatctat cggtgagcga taatgggctt gaaatattaa
      841 cattctgaaa gcttgagcgg aatagtcttt taataaatcc cggatagaaa ttatgttgcc
      901 caaagatttg gacatttttt ctcctctaac gttcaggaat ccagcatgca gccacatgcg
      961 cacatacggt tttttatttg atatcgtttc catttgagct atctcgcatt cgtggtgggg
     1021 gaatatcaaa tccaaagctc cgccgtgcat atcatattgc tggccgaaat atttttctgc
     1081 tatagccgtg tcttcaatat gccagccggg tctgcccttg ccccaagggc tgggccagaa
     1141 gggttcgcca gcttttgctt ttttccacag cgcgaaatcc tgtttatgtt ttttgtctgg
     1201 atcgtgttca attctttccg tttcttccat tttatctaat ttttgatgtg ataatttccc
     1261 gtaatcatgg aaatttttta ctgaaaaata aactccgttt ggtgttatgt aagcacagtc
     1321 tttctttagg aggcgtttaa tttggcttag tatttcgtcg atatggtcgc tggcgcgcgc
     1381 atagttatct ggcgacgtta tatttagggc tttcatgtct ttaaggtatt cgtgttcaaa
     1441 tttatgggct agagctttgg gggttttgcc ttgaagttcg gcgcgcgcga ttattttatc
     1501 atcaacatcg gtaatattct gtaaataccg tacgttataa ccactatggc gtagagtttt
     1561 gaccacggca tcaaagataa caaatactct tccgtggcct atatgtgcca agtcgtaaac
     1621 cgtaggtccg caaacgaata tatcaatctt tttgggcttg agaggcttta gctcttcttt
     1681 ctggcgagta agagtattat agaaagaaaa tttcatgcgt atggatctga atgaccaatt
     1741 taaaattact aattaagttc taataccgaa tcttaaaatg tctaaagcta tataatttag
     1801 aggcgtttta gaactttaga tattgggatt tgtttagaaa ttaaatatta ggaattagaa
     1861 atttgacttt atatccacct tttaaatttg aaaaagagga aggtgaataa acccgaaata
     1921 aaaattacgc tcgtaactat taaaaagcca tttgctgaat ggctaaaagg gatattggaa
     1981 atattagtgg cgaaaatttg cgctatggta tttggcgcca gcattatagc cgtaaaaact
     2041 gttaacagtc ttaaggtcgc attggttttt gttgaaagca gggattgatt cgtttcttcc
     2101 aatgcggcaa tcgtttcttt ctgggtaata agaacgttga ccagtttggc gtgcgattca
     2161 gaaagatatt caagataagg ccgtgcttct ttacccaata aactgggtcc tgaaataagc
     2221 agctctttga atagtccctg cattggttca agtgttctta agaaatctat tattttacgc
     2281 ctggtttggg atatgattat taccatgttt tgctcacggc cacggaatat ctcgtcttct
     2341 atcacggcaa tctggccctc tatattatca agttcagtat tcacgctctc a
//
LOCUS       DATDNZ010000045        14398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1938493, whole genome shotgun sequence.
ACCESSION   DATDNZ010000045 DATDNZ010000000
VERSION     DATDNZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938493"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /gene="glyA"
                     /locus_tag="VI432_01655"
     CDS             complement(<1..647)
                     /gene="glyA"
                     /locus_tag="VI432_01655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173571.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4496838.1"
                     /translation="MRDKIIHGLIKKEIKRQAETLDLIASENFTSVDVLEALGSPLTN
                     KYSEGYPGKRYYPGNVYHDKIENIARERALKTFKLDPNKWSVNVQPHSGSPANVAVYF
                     GLMQPGETFMGMQLSAGGHLTHGHKVSITGKHWKSVQYGVAPDTGLINYNEVERLALE
                     NRPKIIVSGFTAYPQRVDFKRFREIADKVGAYHMVDMAHLAGLIAGGAYPSPFPYA"
     gene            complement(647..937)
                     /locus_tag="VI432_01660"
     CDS             complement(647..937)
                     /locus_tag="VI432_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496839.1"
                     /translation="MKIKRSNKIRIIEFLVIGVLMGVIEDILAISLATDSKINPHVIW
                     IVFIVAIPFAFLSEIVVDHPDFWKRIFPDKNKDNVPDFLEKKIGNANTENKK"
     gene            complement(1160..1600)
                     /locus_tag="VI432_01665"
     CDS             complement(1160..1600)
                     /locus_tag="VI432_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496840.1"
                     /translation="MGAENPEKEGKLSLELGSDGQLRVNGHEVMLVLCDEQQENGWTT
                     VEKIANVLRPLKDLGPDGHEMFPGEDVLRALLKNPHLIPPSWKRDAEGQSICVAFWGG
                     RLNGSSKTGVPILFCNRDGQFESTTLPGGERGRDLTRLTLSALF"
     gene            complement(2206..2877)
                     /locus_tag="VI432_01670"
     CDS             complement(2206..2877)
                     /locus_tag="VI432_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4496841.1"
                     /translation="MKIALITGIGKGIGKALAEKFLREDYFVIGAYYKVCGLEHKNLK
                     AFELDLSDPVSVENCSKRIADLHKGIDIFINNAGVLVDEGESKVVVKKLRETLEINLI
                     GTIDFTERLLPDVNRGGHIINISSRAGSLELATKLISHFPGRYPSYKISKAAINMYTI
                     TLSNRLKDKDIIVSSIHPGRVKTGIGGADADISPEEAAESIYKFAVSKPETGRFWFNG
                     ERVPW"
     gene            complement(3085..4110)
                     /locus_tag="VI432_01675"
     CDS             complement(3085..4110)
                     /locus_tag="VI432_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496842.1"
                     /translation="MHNFEQPPASGENDKELIERQFETAASLFKDGVESGELDLSKDI
                     EILAGNNSHEARALKREALSGVETPDHLREFAESISGDHSDEAGEWRKKIAESPHALD
                     FLARSFAGEKPDSATYDRLVEMSERGAALDPILRGLSGDDSPKAWEIRQKMVDRVLEG
                     DEWPVAGSVFQSLAGVESKEADDMRLKLKTFLGSGESKYSPVRNPGEVNPATAKQIMD
                     GGMVDGADMIAFAHSIAGVDSPSATEFRRDLQNVTDSPAKLEALIVSVTGLDTPEAEG
                     IRNQVLDIKDLKDREYYLGLVAKSLAGTTGERAEMLRLKLQEQGVSRKSLIAGMHKAF
                     VFNHKPL"
     gene            complement(4151..4222)
                     /locus_tag="VI432_01680"
     tRNA            complement(4151..4222)
                     /locus_tag="VI432_01680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4187..4189),aa:His,seq:gtg)
     gene            4302..4766
                     /locus_tag="VI432_01685"
     CDS             4302..4766
                     /locus_tag="VI432_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496843.1"
                     /translation="MKQEKWLEEEPETPPEKEEGKKPDFTARLTEREKDLLERLSPED
                     RQLWEERQEEEIAMGRVLARITGEDESVDPELLRNSFMNSIGQSILSNVDPTFDPNKE
                     DFSWKKFQEKIDENPEIAEAIAPQKQELDPEELKRQEEIRKRRDKDSFRAYS"
     gene            complement(4785..4856)
                     /locus_tag="VI432_01690"
     tRNA            complement(4785..4856)
                     /locus_tag="VI432_01690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4821..4823),aa:Arg,seq:cct)
     gene            4932..5003
                     /locus_tag="VI432_01695"
     tRNA            4932..5003
                     /locus_tag="VI432_01695"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Phe,seq:gaa)
     gene            5011..5337
                     /locus_tag="VI432_01700"
     CDS             5011..5337
                     /locus_tag="VI432_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HEY4496844.1"
                     /translation="MRSHIQHIHQRTKKTKHEPASNKLVYWLDKIVLAAAFIGPLTTF
                     PQIYEIFKNQSAGDISILTWTLFGLLSIPWLIYGLIHKQAPIIVSSILWILVDFIVVF
                     SAVIYS"
     gene            5355..5429
                     /locus_tag="VI432_01705"
     tRNA            5355..5429
                     /locus_tag="VI432_01705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5388..5390,aa:Arg,seq:tct)
     gene            5464..5534
                     /locus_tag="VI432_01710"
     tRNA            5464..5534
                     /locus_tag="VI432_01710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5496..5498,aa:Pro,seq:tgg)
     gene            complement(5590..6021)
                     /locus_tag="VI432_01715"
     CDS             complement(5590..6021)
                     /locus_tag="VI432_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY4496845.1"
                     /translation="MIKNLVWLVGIVFIIIGFLGFIPGNKIVGPGSLFETDAMHNIVH
                     LLSGVIAIAAVLSGEAMARNYLRIFGVVYVLVAILGFMGSSDSILGILVTNNADNWLH
                     AVLAVVFLAVGFIGGKGMKASGSMPTKSNMMGGGMASGSGQ"
     gene            complement(6086..6706)
                     /locus_tag="VI432_01720"
     CDS             complement(6086..6706)
                     /locus_tag="VI432_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496846.1"
                     /translation="MDKAINTTYTLLKFLILVAVAIIILFTLIRAGYGNEPFVPGDFL
                     IARERGALLAEEIVALSQASINNLKDIGSQDAAGNYTLGLNLIIEEIARNEEARNKAI
                     SLSKELEAMAIVLPSIKPSEALNVGTQAILSESQIVQRLINYNDYIYQLLNVLRSRFN
                     KIKNVDSGREIKELIDKMNQEASSINELNEKYKSLMFEFDNLTSSQ"
     gene            6811..6881
                     /locus_tag="VI432_01725"
     tRNA            6811..6881
                     /locus_tag="VI432_01725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6843..6845,aa:Gly,seq:gcc)
     gene            7102..7524
                     /locus_tag="VI432_01730"
     CDS             7102..7524
                     /locus_tag="VI432_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY4496847.1"
                     /translation="MHTIQKLAWAYAILFILVTAIGYVPAFNDESGNLFGLFSLQFYD
                     DLLHVASGIWAGIAAWLSYRASVSYFKIFGVLYGMDGIVGLLTGSGYLDLGIFLNGPV
                     NLDLWTRVATNAPHILIGGFAAYIGFVLSKKFNNKTGS"
     gene            7664..8689
                     /locus_tag="VI432_01735"
     CDS             7664..8689
                     /locus_tag="VI432_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496848.1"
                     /translation="MDSSVTAANSNELNGSDDAKEVLEGLNGYFRNEEQAYLTFPEWY
                     IVYSADEYANFIINNPPSKFPYFRSIGQYWNSYCNIYSITRDNYPFNFGYHVVLFVIG
                     NSYSIENVTKGIYENTIGRIAELISANNTAQEDAYAQKVASEYGKFIHTIPWYEFPFK
                     NKLKELWSETDFFGPNFVRKLERKIILSIEYGFKSIYSWIIKSGAETAYTPQDLEIYA
                     IVKNLSDNILKQNPRTRLVTKIDSELSIILLTRYEEFTQTVPTLTRQGVQFVEIAGND
                     KILISVIAPNGWDLKSNNANALFSMKILTEPNFERVAISVSIEHIHSVLLDLESEGIK
                     LEHIYDY"
     gene            8693..9721
                     /locus_tag="VI432_01740"
     CDS             8693..9721
                     /locus_tag="VI432_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4496849.1"
                     /translation="MRLLRKYFLITGKYIIRAIIALLLIAIGFILFFSWQSDQREAKS
                     NLEAAPSTGRFVQAKDIQVFIQEAGPSNGIPVLLIHGTGAWSEIWRDTMTALAENDLR
                     VIAIDVPPFGFSEKPNGANSYTREKQAERIIGVLDSLDIDKVSLVAHSVGAGPSVETA
                     LMAQDRVKNFVLIDPALGFTSEKEPVFKQNNPSLLLRAFFALKPLRNAALATYGTNPL
                     FTKILFQSFVSNKDAITKEKVSIIQKPLVVKDATRAQGDWLEHLLIFKDSSMASDFSN
                     FQNLRMPVLIIWGDTDSVTPLWQGEKLRDLIPYSELKVMPNVGHIPYIEDAENFNGIL
                     VEFLNQHR"
     gene            9718..11322
                     /locus_tag="VI432_01745"
     CDS             9718..11322
                     /locus_tag="VI432_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4496850.1"
                     /translation="MRKLKQVIKKPITIIAVILIVTVFVIIKKIEYYSADPVGEKNCP
                     PLFPDFVGDPSKQNTIEIDIPQYDFLWAQKGGFINDASCLNKTAVYGVVSIQNTDDIK
                     NALIFAKNKDLKISIAGVKHSMGGHAFYKNALILDMTKFNQMTLNEEDKILNVQSGAT
                     WHDIQNFLHPKLAVKAMQSTDIFTIGGSISVNAHGMDHQAGAIARTIRSMRIMLPDGS
                     IKTVSHIDNPELFNLVIGGYGLFGIVLDVNLEITENVVYQSERKVIDYKDFLDVFGNE
                     IENDKNIGLFYGHLSTAPQSFLKEMILYSYKKVDAPDAKIPPLEEVGSIKLRRFIFNL
                     AKQGSIPMRLKWFAEKYIEPMLENCPVSRNQALSEGEACLVSRNEPMHDSVPYLRNNL
                     KNDTDILHEYFIPRDQFVPYIDEMRTIMTEADVNLLNASVRVVHKENNFLNYSPTDTF
                     SIVLYINQKTDESGNQKMRKVTQELIDLTIKHGGRFFLPYQLHYTTGQLERAYPEFKS
                     FLTMKKKYDPQELFTSTFYKKYSAEP"
     gene            11440..11510
                     /locus_tag="VI432_01750"
     tRNA            11440..11510
                     /locus_tag="VI432_01750"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11472..11474,aa:Trp,seq:cca)
     gene            11640..12521
                     /locus_tag="VI432_01755"
     CDS             11640..12521
                     /locus_tag="VI432_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4496851.1"
                     /translation="MIDHKKGVLMILLSTIAFGSYGTWSRLIGDSFGVFYQGWTRALI
                     ITLILLPILIYRKEIISIQKKDLGWLAIFLLFTTGTQAPLFYAFNHMDISSATLLFFV
                     TMLITMYGVGFIFLSEKVSVIKIISLILAAVGLYVVFSFSVVQFSLLAALMAALNGVA
                     SGGEVAFSKKISSSYSPLYLTWLSWLVIIPTNGVLSLMIGEKQHIPSLDIAWLWQAAY
                     VIASIFAFYLIIAGLKHLEASIGGLLGLLEVLWGITFGIIIFNEILTIKIILGGFLII
                     SAAALPHLYELRKRLST"
     gene            complement(12624..13811)
                     /gene="tyrS"
                     /locus_tag="VI432_01760"
     CDS             complement(12624..13811)
                     /gene="tyrS"
                     /locus_tag="VI432_01760"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY4496852.1"
                     /translation="MTDSDRIKKVLGRGVEEVIELDHLTDALKSGRKLRIKFGIDPTS
                     PDLHLGHSVVLRKLRQFQDMGHTAVLIIGDFTATIGDPTGKTEARKPLTDKEVKTNFK
                     KYIKLAGKILSKKNLEITHNSEWFRKEGSAAMLTLASAATVQQSLHRADFQKRIESGG
                     DLTILELIYPVFQGYDSVKVGADVELGGTDQKFNLLMGRKIQRFYKMKEQDILTVPIL
                     EGLDGVKKMSKSLGNYIALDENPDSMFGKIMSINDNLIIKYFLLCTDLAESEIDKISS
                     EISKKKMNPRDAKVRLAKEIVGIYHDLESANMAEEEFNRVFRSKEKPTEIPIFKTDRS
                     GMDIVAILVGSGLASSKSEASRLIEQKGVRIDGVRVTDPSIIIKKEAIVQAGKRHFVR
                     VVI"
     gene            complement(13841..>14398)
                     /locus_tag="VI432_01765"
     CDS             complement(13841..>14398)
                     /locus_tag="VI432_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY4496853.1"
                     /translation="YDIYSRLLKDRIIFLGGPINDAVANAVIAQMLFLESQDPKKDIE
                     FYINSPGGSVNAGLAIYDTMQYVKPDIKTICIGIAASMGAFLLATGTKGKRFSLPNSE
                     VLLHQIHLGGRGIGGQATEVEIAAKQIMKTKDRMNQLLSKHTGQTFAKIEKDTDRDFW
                     MSADEAKSYGVIDEVIKKSKTSSSS"
BASE COUNT         4363 a         3079 c         2617 g         4339 t
ORIGIN      
        1 gcataaggaa atggactagg atatgccccg ccggctataa ggccagccaa atgagccata
       61 tcgaccatat gatatgcgcc caccttatca gctatctccc tgaatctttt gaaatcaact
      121 ctctgcggat aggcagtgaa tcctgaaact ataattttgg gcctattttc aagggctaaa
      181 cgttcaactt cgttataatt aattaatcct gtatccggcg caactccata ttgaacagac
      241 ttccaatgtt tacctgttat tgagacctta tgtccatgag taaggtgccc tcccgcagaa
      301 agctgcatgc ccataaatgt ttcacccggc tgcataaggc cgaagtacac tgcgacattc
      361 gctggagatc ctgaatgcgg ctgaacattc acgctccatt tattaggatc gagtttaaaa
      421 gttttaagcg ccctctctcg agcgatattt tcaattttgt catgatagac gttgcctgga
      481 taataacgct ttcctggata tccttcggaa tatttattag tgagtggcga gcccaaagcc
      541 tccaatacat ctactgaagt gaaattttca gaggctatca aatcaagcgt ttctgcttga
      601 cgcttaatct ctttttttat caaaccatgt ataattttgt ccctcattat tttttatttt
      661 ccgtattcgc attcccaatc tttttttcta aaaaatcggg tacgttatct ttatttttat
      721 ctgggaatat tctcttccaa aaatctggat gatctactac tatctcgctt aaaaacgcga
      781 acggtatcgc aacaataaac acaatccaaa taacatgcgg gtttattttg gaatcagtcg
      841 ccaacgatat agctaaaata tcctctataa cgcccattaa aaccccgatt acaagaaact
      901 caattatcct tatcttatta gaacgcttaa tcttcataag agtaattata cttctatgaa
      961 taaaaatctg aaaactatct aatcaaactg attatggact tcgatatagg aaagcctatt
     1021 aaagagtcta aaagtattaa tcctccgata agaaatatta aaaaaaccat taattcgttt
     1081 ttatctccca tatattttat taaattttat atcaattaga tcgcccgtat actatgatac
     1141 gggctttact tgcgttccat tagaataagg cgctcagagt gagtcgcgtc aaatctctac
     1201 ccctctctcc tcctggaaga gtggtggatt cgaattgccc atctctgttg caaaaaagga
     1261 taggtactcc ggtctttgaa gaaccgttca ggcgtcctcc ccagaaagct acgcatatag
     1321 actgaccttc agcgtccctc ttccaactcg ggggaataag atgaggattc ttcagtaatg
     1381 cccgcaaaac atcttctccg gggaacattt catgtccatc cggacccaaa tctttaagag
     1441 gacgcaaaac atttgcaatc ttctctacag ttgtccagcc attctcttgc tgttcatcac
     1501 agagcacaag cataacttcg tgcccattga cgcgcagctg gccgtcagat cccaactcta
     1561 gactcaattt accttctttt tctggatttt cagcgcccat tcagattccc tccgctgctc
     1621 cttacccgct attaaagagc tttattatat ttatctcatt tcgaaaacga aatgtcaatt
     1681 tgataattag aaagaaattg agtcgctcta ttcggcgacc cataactctc ctagatatgt
     1741 ctgaatgctc cgatgtggac cttaagaagc tcgccaggat gaaacatttg catatgtgta
     1801 acctgagctt tgacatcaca ctctctcagt ctcaaggggc agcatggaca cctgatcact
     1861 tcttcgtgtc tcttgttgcg catcctaaac actccgatcg tggttctatt tagataatag
     1921 acgatcatcg acaaaaagaa aaggctggag tgctcattcc agccggacta agtaaccgct
     1981 actcctttca tacgaacaga aagaccgcac cttggacata ccgaccattc agcaattttc
     2041 tgagaatcag tcgaaacaat tttatgccca caactctggc atacgatttc gaagtattct
     2101 cctctcatgt ctataccaac gatctatttt aattctaggg tttttatata atctttcaac
     2161 tttaaaattc tgtaagttga ttcatttata ttcccttagc ttatctcacc aaggcactct
     2221 ctctccatta aaccaaaacc ttcctgtttc cggttttgaa accgcaaatt tataaataga
     2281 ttccgccgcc tcttctggcg agatatctgc atcagcccct cctatgcccg tcttaactct
     2341 tcctggatga atagatgaga caataatatc cttgtctttt aatctattag acaaagtaat
     2401 cgtatacata ttaatagctg ctttagaaat tttataactc gggtatcttc ctggaaaatg
     2461 acttattaat tttgtcgcta gctcaagcga tccggctcta gatgatatgt tgattatatg
     2521 tccgccacga ttaacatctg gcaacaatct ttcggtaaaa tcaattgttc cgataagatt
     2581 tatctccagc gtctctctaa gttttttgac aacaacttta gattcgcctt catcgacaag
     2641 cacgcccgcg ttgtttataa aaatatctat gcccttgtga agatccgcga ttctttttga
     2701 gcaattttca acgctaaccg gatcgctcaa atctaattca aaagctttca aatttttatg
     2761 ctctaaaccg cagactttat aatatgcgcc gattacaaaa taatcctctc taagaaattt
     2821 ttcggcgagc gcctttccta tgcccttacc aatcccggtt attaaagcta ttttcataga
     2881 attctattaa tcattcgcct attcacaaca ttcttctgtt atccttattc tatccttttt
     2941 ctctatatac ccagatctcc aaactgttca aatggcatat taaaataata tcataatttt
     3001 acctctccaa atcaaaattg cgttaatcta taaaattaaa tagagggttt aaaccctctt
     3061 tattttatcg actgccacgt gacactaaag cggtttgtga ttgaacacaa aagccttatg
     3121 cattccagcg atcaagcttt ttcttgaaac tccttgctct tgcaacttaa gcctaagcat
     3181 ctctgcgcgc tctccagttg taccagccaa actcttcgcg acaagcccaa ggtaatattc
     3241 tctatcctta agatccttaa tatcaagaac ttggttgcga ataccctcag cctctggagt
     3301 atccaatcca gtcacgctga caatcaatgc ctcaagcttt gccggactgt ctgtgacatt
     3361 ttgtaaatcc cttctgaatt cagtcgcaga tggactgtca acgcccgcga ttgaatgcgc
     3421 gaaagcgatc atatcggctc catcaaccat tcctccgtcc ataatttgtt tagccgtcgc
     3481 cggattcacc tctcctggat ttctaacagg ggaatattta gactcgcctg atcccaaaaa
     3541 ggtttttagc ttcaaacgca tatcgtcggc ctctttgctt tcaactcctg ccagactttg
     3601 aaagacagag cccgcaaccg gccattcatc accttctaaa acgcggtcca ccatcttctg
     3661 cctgatctcc caagcttttg gagaatcgtc gcctgatagc ccgcgaagaa tagggtcaag
     3721 ggctgcgcca cgctcagaca tttctaccaa tctatcataa gttgctgaat cgggtttttc
     3781 accggcaaag ctcctagcca agaagtcgag agcatgggga gattcagcaa tcttcttcct
     3841 ccattcacca gcttcatccg aatgatctcc ggagatgctc tcagcgaatt ctcgcaaatg
     3901 atccggtgtt tcaacgccgc ttaaagcttc tcgtttcaat gcgcgcgctt catgagaatt
     3961 gttgcctgca aggatttcaa tatcttttga aagatctaac tctccactct cgacgccatc
     4021 cttaaacaac gacgctgctg tttcaaattg tctttctata agctctttgt cgttttcccc
     4081 cgatgcagga ggctgttcaa agttgtgcat acaccaacag cataagctaa ctatttcatt
     4141 aagtaaaatg ggtgcctaac gggaatcgaa cccgtatcaa gagttccaca aactcttgtg
     4201 ttaaccatta caccataggc accataactt cgtgcattat aatattacgc cccaattaat
     4261 caaactggca attataaaat tacggttgta ttatttaggc tatgaaacag gaaaaatggc
     4321 ttgaggaaga accggagacg ccaccagaaa aagaagaggg gaaaaagccg gattttactg
     4381 cgcgccttac cgaacgggaa aaagacttgc tcgaaagact ttctccagag gacagacaac
     4441 tttgggaaga acgacaagaa gaagaaatag ctatggggag ggtccttgcg agaatcaccg
     4501 gcgaggacga aagcgttgat cctgaattat tgagaaattc atttatgaat agtatcggcc
     4561 aatcaatact tagcaatgtc gaccctacat ttgaccccaa caaagaagat ttctcttgga
     4621 aaaaatttca agaaaagatc gatgaaaatc cggagatagc tgaagcgata gctccgcaaa
     4681 aacaagaact agatcctgag gagttgaaaa gacaagaaga aatacgaaaa cgaagagata
     4741 aagacagctt cagggcttat tcgtaaaata cgatcccaac attgtgcctt cgagaggaat
     4801 cgaacctcta tcgactcgtt aggagtgagc cgttctgtcc attgaactac gaaggccttc
     4861 tttgatgtag gataatttgt ttatttgtat attgcaattt aataataatg gcaataatat
     4921 ctaactacat cgccgcggta gctcagtggt agagcgcagc cctgaagagg ctggcgtcgg
     4981 gggtccgatt ccctcccgcg gcacaactat atgagaagtc atatccaaca catacatcaa
     5041 agaactaaaa agacgaaaca cgaaccagca tcaaataagt tggtttactg gctggataaa
     5101 atagttctcg ccgccgcatt tatcggaccg cttacgactt tcccgcaaat atatgaaata
     5161 tttaagaatc aaagcgccgg cgacatatcg atactcacct ggactctatt cggattatta
     5221 tctatacctt ggcttatata cgggcttatc cataaacaag ctccaataat agtttcttct
     5281 atactttgga ttttagtaga ctttattgta gtattttcag cggtgatata ttcttaaatt
     5341 taccgaacaa ctacgcctct gtagttcaat ggacagaata agggacttct aatccccaga
     5401 tggaggttcg attcctcccg gaggcaccaa cttcgatgag ctcaagatgg gccgcgaaga
     5461 aaacggagag tagtataatg gtagtataca tgttttggga acatgaggct cgggttcgat
     5521 tcccggctct ccgacaagat aaaaacagcc cgatggggct gtttttatta taaacaaatt
     5581 atatcagtgt tattgaccag acccactggc cattccccca cccatcatat tggattttgt
     5641 gggcatagaa ccgcttgcct tcattccttt ccctcctata aatccgaccg caagaaaaac
     5701 cacagcgaga acagcatgca gccaattatc agcattatta gtaactaaaa tgcctaaaat
     5761 ggagtccgaa ctgcccataa atcccaaaat agcaaccaat acatatacaa ctccaaaaat
     5821 tcgcaaataa tttcttgcca tcgcctcgcc agaaagaacc gcagcaatag caattacacc
     5881 agacaacaaa tgaactatgt tatgcatggc atctgtctca aatagcgagc cggggccgac
     5941 aatcttgttt ccgggtataa aacccaaaaa cccgataata atgaatacta taccgacaag
     6001 ccaaactaaa tttttaatca tagaataaaa tagctaaatc gacctttttt tatattatac
     6061 aataaactta ttttaggata agaaattatt gcgaagacgt aagattatca aattcaaaca
     6121 tcagactttt atatttctcg tttagctcat tgatagaaga cgcttcttga ttcattttat
     6181 ctatcaattc ttttatttct cttccgctat caacgttttt tattttatta aatctggaac
     6241 gcaagacatt taaaagctga taaatataat cgttatagtt aatcagtctc tgcactatct
     6301 gcgactcgct tagaatagct tgagtgccga cattcaaagc ttcacttggc ttgatgctcg
     6361 ggagaacaat cgccatagcc tctaattctt tagacaggct gatagctttg tttctcgctt
     6421 cttcgtttct agctatttcc tcaattatca gatttaaacc aagtgtataa ttgccagctg
     6481 cgtcttgaga tcctatgtct tttaaattat taatagacgc ttgagatagg gctacaatct
     6541 cttctgcaag taaggcgcct ctctcgcgag caatcaaaaa gtcgcctggc acaaaaggct
     6601 cattgccata gccagccctt atcaaggtaa aaaggatgat gatggccacc gcaacaagaa
     6661 tcaaaaattt aagcagggtg tatgttgtat taatagcttt atccattaat tatcaattga
     6721 ttctaaaata gattatgtcc tttttcaatt taaaaatagt ttgaaattta tatgaaaaat
     6781 atatatcatc taatctaccg cgtagtgaaa gcgggattag tatagtggca gtacgctacc
     6841 ttgccaaggt aatggcgccg gttcgattcc ggtatcccgc tcaagaatta gactttcaga
     6901 taaaagacca gctttttggc aggcggagcg tagcaccgcc tgcgaattcg tcggtcaaaa
     6961 gcctgagagc catatcctgg agcgtcttta caattttgcc ccagattaat ttatcataga
     7021 ctcaataaat gcggtctcat cactcacgca gaaaaagcgc ggttggtgca atggaattaa
     7081 aatttctgtt ataattgcgg catgcacaca attcaaaaac ttgcttgggc ttatgcgatc
     7141 ctttttatac tagtaacagc cattggatac gtgccagctt ttaatgacga gagcggcaat
     7201 ttattcggct tattctcgct acaattttat gacgatttat tacatgttgc gtcaggcatt
     7261 tgggctggaa tagccgcatg gctttcgtat cgcgcttcgg tttcttactt taaaatcttt
     7321 ggggtcctat acggaatgga tggaatagta ggacttctaa ccggaagcgg atacttagat
     7381 ttaggaattt ttctcaatgg ccctgtaaac ctagatttgt ggacaagagt cgcaaccaat
     7441 gctccccaca tattgatagg aggctttgca gcatatatag gttttgtttt aagtaaaaaa
     7501 tttaataaca aaacaggttc ataatccaaa gaaatacttg agaaaatttt taaacataat
     7561 ttggcgttca cgcgcagtca gggctattac tttggtaatt ttgtttatca ttgtcattca
     7621 agcgcttatt ttttcaagta tgtgggtttt atgtcgtccc ggtgtggata gcagtgtcac
     7681 cgcagcgaac tcaaatgagc taaatggtag cgatgacgcg aaggaggttt tagaagggct
     7741 taatggatat tttcgtaatg aggagcaagc atatctgact tttccagaat ggtatattgt
     7801 ttatagcgcg gatgaatatg ccaattttat tataaacaat ccaccgagta aatttcctta
     7861 ctttcgatct atcggacaat actggaacag ttattgcaat atttattcaa tcacaagaga
     7921 taattaccct ttcaattttg gataccacgt tgtcctgttt gttataggca acagttattc
     7981 aattgaaaat gttacgaagg ggatttacga gaacacaatc ggaagaatcg cggagttgat
     8041 cagcgctaat aacacggctc aagaggatgc atatgcgcaa aaagtggcta gcgaatatgg
     8101 aaaatttata cacacaattc cgtggtatga atttcctttt aagaataaac tcaaggaact
     8161 ttggagtgaa acagatttct tcggaccaaa ttttgtgcga aaattggaaa gaaaaattat
     8221 tttaagtatt gagtacggtt ttaagtcgat ctacagttgg attattaaaa gcggagctga
     8281 aaccgcgtat actccacaag atttggagat atacgcaatt gtaaaaaatc tatcagataa
     8341 tattttaaaa caaaatccac gaacccgact agtcacgaag attgactcag agctttcaat
     8401 tatccttctt actagatatg aggaattcac acagacagta cccactctta cgagacaagg
     8461 ggtacaattt gtcgaaattg ccgggaatga caaaatatta atatctgtta ttgctcctaa
     8521 cggttgggac ctaaaatcaa acaatgccaa cgcattattt tcaatgaaaa ttctaacaga
     8581 gccaaatttc gaacgtgtcg cgataagcgt ttcaatagag cacatccatt ctgttctgct
     8641 tgatttagag agcgagggga taaaacttga acatatttat gattattaat ttatgagact
     8701 tttaagaaaa tattttttaa taactggtaa atatataata cgggccatca tagctttatt
     8761 gcttatagca ataggcttta tactcttttt tagttggcaa tcagaccaaa gagaggccaa
     8821 aagcaatcta gaggcagcgc cttcaacagg aaggtttgtg caggcgaagg atatacaagt
     8881 ttttattcaa gaggcgggac cgtcaaacgg aattcctgtc ttacttattc acggaacggg
     8941 cgcatggagt gaaatatggc gggatacaat gaccgccctt gccgaaaacg acttgcgcgt
     9001 gattgcgatc gatgttcctc ctttcggatt ttcagaaaaa ccaaatggcg ccaattcata
     9061 caccagagaa aaacaagctg aacgtattat cggggtattg gattcgcttg atatagacaa
     9121 ggtcagtctc gtcgcccatt cagttggagc aggaccctcg gttgaaaccg cattaatggc
     9181 gcaagataga gttaaaaatt ttgtgcttat cgatccggca ttaggattta cttctgaaaa
     9241 agagccggtg tttaaacaaa ataatccctc attattactt cgcgcttttt ttgccctcaa
     9301 accactgcgg aatgcagctc tagcaaccta tggaacaaac ccacttttta caaaaatatt
     9361 atttcaatcg tttgtgtcca ataaagatgc gatcaccaaa gaaaaagtct caatcataca
     9421 aaaaccacta gtggtgaagg atgcaacaag ggctcaagga gactggctag aacaccttct
     9481 tatatttaaa gacagctcta tggcaagtga tttctcaaat ttccaaaatc taagaatgcc
     9541 tgttttaata atatggggag ataccgacag tgttacgccc ttatggcaag gggaaaaatt
     9601 acgagacttg attccctatt cagaattaaa agtcatgccg aacgtaggac atattccgta
     9661 tatagaagat gcggaaaatt ttaacggcat tctagttgaa tttttaaatc aacacagatg
     9721 agaaaactca aacaagtaat taaaaagccc ataacaatta ttgcggtaat acttatagtc
     9781 acagtttttg ttattataaa gaaaatcgaa tactattctg ccgatccagt gggagaaaaa
     9841 aattgtccac cgctttttcc agattttgtc ggcgatccgt ctaaacaaaa cactattgaa
     9901 atcgatatac ctcaatatga cttcctgtgg gctcagaagg ggggctttat caatgacgca
     9961 agctgcctta acaaaaccgc agtgtatgga gttgtttcta tacaaaacac agatgatatc
    10021 aaaaatgctt tgatttttgc gaaaaataaa gatctcaaaa tctctattgc cggtgttaag
    10081 cacagtatgg gagggcatgc gttttataaa aatgccctga ttctggatat gacaaaattt
    10141 aatcaaatga cgcttaatga agaagataaa attttaaacg tgcaaagcgg cgcaacatgg
    10201 cacgatatac aaaattttct ccatccgaaa ttagcagtca aagccatgca gtctactgat
    10261 atttttacta ttggcggctc tatttccgta aacgctcacg ggatggacca tcaagccgga
    10321 gcgattgcga gaaccattcg ttctatgcga attatgctgc cagacggttc tattaaaact
    10381 gtcagccaca ttgataatcc agagctattt aatttggtga taggcggcta tggtcttttc
    10441 gggattgtac ttgatgttaa tttagaaatt acagaaaacg ttgtttatca atccgaacga
    10501 aaggtaatcg actataaaga ctttttagat gtattcggta acgaaatcga gaacgataaa
    10561 aatattggcc tattctatgg ccatctttct acggcgccgc aatccttctt aaaagagatg
    10621 attttatata gttataaaaa agttgatgcg cctgacgcta aaattcctcc cctcgaagaa
    10681 gtaggtagta ttaagttgcg aagatttata tttaatctcg caaagcaggg ttcaatccca
    10741 atgagattga aatggttcgc cgagaaatat attgagccga tgctagaaaa ttgtccagtt
    10801 tcaagaaatc aagcccttag tgaaggtgag gcttgcttgg tatcgcgaaa tgaacccatg
    10861 catgattcag tcccatatct ccgcaataat ctaaaaaatg acaccgatat tttgcatgag
    10921 tattttattc cacgtgacca atttgttcca tacattgatg agatgaggac aataatgact
    10981 gaggctgatg taaatctgct taatgcgtct gtacgcgtag tccacaaaga aaataatttt
    11041 ttgaactact ctccaacaga caccttctcc atcgttttat atataaatca gaaaacggat
    11101 gaaagcggaa atcagaaaat gagaaaagtc actcaagaac ttatagatct cacaatcaaa
    11161 catggcggca gatttttctt gccatatcaa ctacattaca caactggcca gctagaacgg
    11221 gcgtacccag aatttaaatc gtttttgacg atgaaaaaga aatatgatcc acaagaacta
    11281 tttacaagta cgttttataa aaagtattca gcggaaccat aatagattat accaacgcta
    11341 ctttctctgc ggaacaaagc agcttccttt gtttaggaat ttttgataaa gtaggttcta
    11401 gctctatcat ataggtactc aaagattttt aatagaattg ggagcttagt taagtggtat
    11461 aatagtggtc tccaaaacca cggtcgaagg ttcgattcct tcagctcccg cagagttgta
    11521 ataagtcgtc ccgtacgaga tgcttcactt attagagcgc atagaaaaac tgaacgcaac
    11581 aattttattt gtaactttaa actaagattt tctctttcaa gactatgcac aataccaata
    11641 tgatagatca caaaaaggga gtcttaatga tcctcctatc aacgatagct ttcgggagct
    11701 atggaacttg gtctcgcctt attggcgact catttggcgt gttttaccaa ggatggactc
    11761 gagcattaat aatcacatta atacttcttc caattctgat ctaccgaaag gaaattatat
    11821 caatacaaaa gaaagatctt ggttggctag caatatttct attattcaca acaggaactc
    11881 aagctcctct tttctatgca tttaatcata tggatataag ctcggctact ctattgtttt
    11941 ttgttacgat gctaatcacg atgtatggag ttggatttat ttttctctca gaaaaagtct
    12001 ctgtgattaa aattatatct ttaatactag ctgccgttgg tctatatgta gtcttttctt
    12061 tctctgtagt tcaattttct cttttagcgg cgctgatggc tgcactaaat ggggtcgcta
    12121 gtggtggcga agtagccttt tcaaaaaaga tatcttcgtc ttattctcca ctttatctaa
    12181 cgtggctaag ctggttggtg atcatcccta caaatggggt actgtcatta atgatagggg
    12241 agaaacagca tattccatct ctggatatag cttggctatg gcaagcagct tacgttatag
    12301 caagtatatt cgctttctat ttaatcatcg ctggtttaaa acatttggaa gcgagtatag
    12361 gagggttgct tggtctgttg gaagttctct ggggaataac ttttggaata ataatattta
    12421 atgaaatttt aactatcaag ataatcctcg gtggattctt gattatttct gcggccgcgc
    12481 ttccacattt atacgaattg agaaaaaggc tatccacata gcttatttta ttccttatac
    12541 gcagattgtc tgtatttccg gctcattacg tagttaaaat tgctttgaac agaaaacaaa
    12601 atacttaaag ttaataagct agattaaata acaactctta caaagtgcct tttccccgcc
    12661 tgaacaatag cttccttttt aattattatc gaagggtcag tcaccctaac cccgtctata
    12721 cggacccctt tttgctcgat taaacgcgac gcctcgcttt tacttgaagc aaggcctgaa
    12781 ccgacaagaa tcgctactat atccatgcca gatctgtcag ttttaaaaat agggatttct
    12841 gttggttttt cttttgatct aaaaactcta ttaaattctt cttctgccat attcgccgac
    12901 tccagatcgt gatatatacc aacaatttct tttgccaacc tgactttagc atctctggga
    12961 ttcatctttt ttttactaat ctcactcgag attttatcga tttctgactc tgccaaatcg
    13021 gtgcacaata ggaagtattt aataatcaag ttgtcgttaa tagacatgat tttaccaaac
    13081 atcgaatcag gattttcatc aagggcgata taattaccga ggctcttgct catttttttc
    13141 actccgtcta acccttctaa aatcggcacg gtaagaatgt cctgttcttt cattttataa
    13201 aaacgctgta tcttgcgccc catcaataaa ttaaatttct gatctgtccc gcccaactca
    13261 acgtcagccc caactttcac cgaatcgtat ccctggaata ctgggtaaat aagctccaga
    13321 attgttaagt ctccaccgga ctcaatcctt ttttgaaaat ccgccctatg taaagactgt
    13381 tgaaccgtag cggctgatgc gagcgttaac atcgcagcag agccctcttt cctaaaccac
    13441 tcgctattat gcgtgatttc taaatttttt ttacttaaaa ttttaccagc aagctttata
    13501 tattttttaa aattagtttt aacctcttta tccgtcaagg gttttctggc ttccgtcttt
    13561 ccggtcggat cacctatagt tgctgtgaaa tctccgatta tcaaaactgc ggtatgcccc
    13621 atatcttgaa attgtcttaa tttacgaaga accacagaat gccccagatg cagatcgggg
    13681 ctcgttggat caattccaaa tttgatgcgt aatttacgtc cagattttaa agcatcggtt
    13741 aagtgatcga gctctataac ctcctcaact ccacgtccta aaaccttttt tatcctgtcc
    13801 gaatcagtaa tgactgccat aagttaattc taagacaaaa ctaagaacta gacgatgttt
    13861 ttgatttctt aataacttcg tctataactc cgtatgattt agcctcatcc gcgctcatcc
    13921 aaaaatccct gtccgtatct ttctctattt tagcaaaggt ttggccggta tgtttagata
    13981 aaagttgatt catcctatct ttagtcttca taatctgttt cgctgcaatc tccacctcag
    14041 tcgcctgacc acctatccct ctcccaccta aatggatttg atgcaatagc acttcagaat
    14101 tcggcaggga aaatctttta ccttttgttc ctgtcgccaa aagaaacgca cccattgaag
    14161 ccgcaattcc gatacaaatt gttttaatat ccggctttac atattgcatg gtgtcataaa
    14221 tagcgaggcc ggcgttcacc gatccaccag gagaattaat ataaaactca atatccttct
    14281 ttggatcctg actctctaaa aaaagcatct gtgcgatcac ggcgtttgca acggcatcat
    14341 ttatcggccc gcctaaaaag atgattctat ccttcaaaag acgcgaatag atgtcgta
//
LOCUS       DATDNZ010000046         4265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2464933, whole genome shotgun sequence.
ACCESSION   DATDNZ010000046 DATDNZ010000000
VERSION     DATDNZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464933"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VI432_01770"
     CDS             <1..1093
                     /locus_tag="VI432_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY4496854.1"
                     /translation="DIKTLYNCSMLGKGLESLIPQKGSSVSDSDNTDDASTDQLSGVS
                     GGAILSDAKPEEEIIKTVKENSHLPVKPVDAFYKKGDAIFHIEIDKIRPNPHQPRKYF
                     DEEALKDLASSIREFGVIQPIVVSKIEKESDTGTTVEYELIAGERRLMASKRAGLSSI
                     PAIIKESSSSRVKLEMAVIENIQRADLSPIEKAKAFARLQDEFGLTQREIAVRLGKSR
                     EVVANAVRLLNLPTEVQEALAGGKIGESQARMLLSIEDILKQKEIFQSIIRDNLSVRD
                     IKSKIQKIKLDKPIAIQNINPEIEYIREQLEELFGTKVDVKNDDGNSGKIMINFYSEE
                     ELRAITDKLLKTNKSNPEPSFTEDDNLSL"
     gene            complement(1065..2750)
                     /locus_tag="VI432_01775"
     CDS             complement(1065..2750)
                     /locus_tag="VI432_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY4496855.1"
                     /translation="MALNDTLLENNTIKKAYNLIHDVYGEKRRASGESYIEHSVATAE
                     TLAEWGLNETTIAAGLLHDIAEHADYTIKDIQKNFGKEISFLVDGVTRISKFNYQGGE
                     EQAETIRKMVLAMAQDVRVMLIKLAARKHNIANMDKLHPEKKKNLALRTLEIYAPLAN
                     RLGMSKLSGEMEDAVFPVMYPEEHEWLIKNIKYKYEEHEKYLSEIRPQVEAELKRAAI
                     KPEFIDFRAKRHASLYKKLLRYELDIERIYDLVAFRIIVKDLQDCYAVLGVLHELWSP
                     LPGRIKDYIATPKPNGYQSLHTTVIGPKEKIVEFQIRTVDMHHKAENGVAANWAYTEN
                     KKNKSYMRKRASLANLKQVAWVEKLRSWQKEFTDPKEFLDSLKIDFLEDRIFVTTPKG
                     DVIDLPNGSTPIDFAYQIHSSLGNECIGAKINGKLMPLDYKLKSQDVVEIIRQKNKKP
                     SENWLNFVVTSSARHFIKKTVKSRRRFFSSMKKFSELKVTIKDKIGLLKEISSVISSH
                     KINIVSINSSQDTEGKFHTIAFKCETADRNKIQKLILRLKKIKEVKEIDYRLL"
     gene            2831..3358
                     /locus_tag="VI432_01780"
     CDS             2831..3358
                     /locus_tag="VI432_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4496856.1"
                     /translation="MKVDIVKKAASFAKERHKHQKRKTGEPYFIHPKNVARTVSNWTK
                     DKNVIAAGYLHDVIEDTGADYEDIEERFGKKVADYVSLMSRDMRLPKNKSLSLFRRQL
                     GSAPPEVKLIAAADMENNASSIADTDFMKSWFNKINTMMPYLKRGNMGRYKKGILSLL
                     SRVEKAEDKFLSNLR"
     gene            complement(3345..>4265)
                     /locus_tag="VI432_01785"
     CDS             complement(3345..>4265)
                     /locus_tag="VI432_01785"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY4496857.1"
                     /translation="DLIWRRFIASQLPPALFDTTAIDIKCGKYTLRANGTTMDFDGFL
                     KVWPTKFEDKELPELKEGEKPKLIEIIPIQHFTEPPPRFNEASLIKTLEANGIGRPST
                     YASIISVIQTRNYVSKENRRLYPTETGELVNKVLVENFSDVFDIGFTADMENKLDEVA
                     NGSENWQELIKNFYNPFSKKLEEKYEEVKKLTVDEKTDELCDKCGKPMVIKFSRFGRF
                     LGCSGFPDCKNIKKLQKEAPKLIGLKCSKCKEGDIIERRVTRGRAKGKIFWGCSRYPN
                     CDFATWENPLKKDEEEDVEKKDEEEEVNED"
BASE COUNT         1194 a          801 c          837 g         1433 t
ORIGIN      
        1 tgatattaaa accctataca attgttctat gcttggaaaa ggtttagagt ctttaattcc
       61 gcaaaaaggg agttccgttt cggattcgga caacacagat gatgcttcaa cggatcagct
      121 ctctggtgtt tctggggggg ctattttgag cgatgcaaag cccgaagagg aaataataaa
      181 aaccgttaaa gaaaattctc atttaccagt taaaccagtt gatgcgttct ataaaaaagg
      241 agacgcgata ttccatattg agattgataa aataaggccc aatccgcacc agcccagaaa
      301 atattttgat gaagaagcct taaaagatct ggcgtcttcg ataagggaat tcggtgtgat
      361 ccaaccgatc gtcgtttcta agatagaaaa agagtcggac accgggacga ccgttgaata
      421 tgagcttatt gccggagaga gaaggttgat ggcttcaaaa agagctgggc tttcaagtat
      481 cccggctatt ataaaagagt cgtcttcttc aagggtaaag cttgaaatgg ctgttattga
      541 gaacattcag agagccgatt tgagcccgat agaaaaagct aaggcttttg ccagacttca
      601 agatgaattc ggcctaactc aacgggagat agccgttaga ttgggcaaaa gcagagaggt
      661 tgtcgcaaat gccgtcagac ttcttaattt accgactgaa gttcaagaag cgcttgcagg
      721 cggtaagata ggagaaagcc aggcgagaat gcttctctca atagaggata tcttaaaaca
      781 gaaggaaatt tttcaatcta tcattagaga taacttaagc gttagagata ttaaatctaa
      841 aattcagaag ataaaacttg ataagccgat cgcaattcaa aatattaatc cggagattga
      901 atatattaga gagcagctag aagaactttt tggtacaaaa gttgatgtta agaacgatga
      961 cggcaatagc ggtaaaatta tgattaattt ttattccgaa gaggaattaa gagctattac
     1021 tgacaaacta ttaaagacca ataagagtaa tccagagcct tcttttacag aagacgataa
     1081 tctatctctt taacttcttt tatttttttg agtctaagga ttagtttttg aattttattc
     1141 ctatcggcgg tttcgcattt aaacgctatt gtatggaatt taccttctgt gtcttgtgat
     1201 gaatttatag aaacaatatt tattttatga gaagatataa ccgaagagat ttctttgaga
     1261 agccctattt tatctttgat ggttactttc aattcgctaa attttttcat tgaagagaaa
     1321 aaccttctcc tagacttaac ggttttttta ataaaatgcc tagccgaaga tgtgactaca
     1381 aaattcaacc agttttcaga gggtttttta tttttttggc gtattatctc cactacgtct
     1441 tgagatttta atttataatc aagtggcatt aattttccat tgatcttcgc tccgatacat
     1501 tcgttaccca aactactgtg tatttggtat gcaaaatcaa ttggagtcga gccgttgggt
     1561 aaatctataa catcaccttt tggagttgtg acgaatattc tatcttcaag gaagtcaatt
     1621 tttagagaat cgagaaattc cttaggatcg gtaaattctt tttgccatga acgtaatttt
     1681 tctacccatg caacctgttt caagttggct aagcttgccc ttttccgcat ataactttta
     1741 tttttcttgt tctcggtata cgcccagtta gcagctaccc cattttcagc tttatgatgc
     1801 atatcgaccg ttcttatttg aaactcaact atcttctctt tggggccgat aacggttgtg
     1861 tgcaggcttt ggtaaccatt tggtttggga gttgctatat agtcttttat tctgcccgga
     1921 agaggggacc acaattcatg taagacgccc aatactgcgt agcaatcttg aagatctttt
     1981 actatgattc tgaacgcaac caagtcatag attctttcta tgtccaactc gtatcttaat
     2041 agttttttat ataagcttgc gtggcgtttg gcgcgaaaat ctatgaattc tggtttgatg
     2101 gcggctcttt ttaattctgc ttcaacttga gggcgaattt cagatagata tttttcatgc
     2161 tcctcatatt tgtatttaat gtttttaatc agccattcgt gctcttcggg atacattact
     2221 ggaaagaccg cgtcttccat ctctccgctt aatttgctca tccctaatcg gttagcaagt
     2281 ggcgcatata tctcgagcgt tcttagggca agattttttt tcttctctgg gtggagtttg
     2341 tccatattcg ctatattgtg ttttctggcg gcgagcttaa taagcattac gcgcacgtcc
     2401 tgggccatcg cgagcaccat ttttcttatg gtctctgctt gttcttcacc cccttgatag
     2461 ttgaatttac tgattctcgt gactccatcc acaagaaatg aaatctcttt cccaaaattt
     2521 ttttgtatgt cttttatcgt atagtctgcg tgttctgcta tgtcgtgcaa tagaccggcg
     2581 gctatagttg tttcattcag tccccattca gcaagagttt cagcggtagc cacagagtgt
     2641 tctatatacg attctccgct ggcgcgccgt ttttcgccat aaacatcatg gattaaatta
     2701 taagctttct taattgtatt gttttcaagc agtgtatcgt taagagccat cacagtcttt
     2761 ttatatttaa aatatcatcg tgtcaaacaa atacgcttct catttttgaa tcgtgatata
     2821 atggcgatat atgaaagttg atattgtaaa aaaggcggct tcatttgcga aagaaaggca
     2881 taagcatcag aaaagaaaga cgggcgaacc ttattttatc catccgaaaa acgtggctag
     2941 aaccgtaagt aattggacta aagataaaaa tgttattgcg gccggatatc tccacgatgt
     3001 gatagaggat accggcgcag attatgagga tatagaggag cgttttggta agaaggtggc
     3061 tgattacgtt tctctcatgt cgagagatat gcgcttgcct aaaaataaga gcttgagtct
     3121 ttttcggagg cagttaggtt ctgctccgcc agaggtaaag ctcatcgccg ccgctgatat
     3181 ggaaaataat gcgagctcaa tagccgacac cgactttatg aagtcttggt ttaataaaat
     3241 caatacaatg atgccttact taaagcgtgg caacatgggc agatacaaaa aaggaatttt
     3301 aagtctttta tcgagagttg aaaaagctga agataaattt ctatctaatc ttcgttaact
     3361 tcctcttcct cgtctttttt ctctacatcc tcttcctcat cttttttaag cggattttcc
     3421 caggttgcaa aatcacagtt aggatatcta gagcatcccc aaaatatttt tcctttagct
     3481 cttcctcgag tgactcggcg ttcaatgata tctccttctt tgcattttga gcattttaac
     3541 ccgattaatt taggagcttc tttttggagt tttttaatgt ttttacaatc tggaaatcct
     3601 gaacatccca agaatctgcc aaatctgctg aatttaatga ccattggttt gccgcattta
     3661 tcacagagtt cgtctgtttt ttcgtcaacc gtaagttttt taacctcttc atatttttct
     3721 tcaagcttct tgctaaaagg gttatagaaa ttcttaataa gttcttgcca attttcgctt
     3781 ccgttggcca cttcatcaag tttattttcc atgtctgcgg taaatcctat atcaaaaaca
     3841 tcggagaaat tttctacaag aactttatta accaattctc cggtttcggt agggtataac
     3901 cttctatttt ctttagaaac ataatttctt gtttgaatta ccgagataat gctggcgtat
     3961 gtagacggcc ttcctatccc gttcgcctca agagtcttta taagacttgc ttcattaaat
     4021 cttggcggcg gttcggtaaa gtgttggatg gggataatct ctattagttt tggtttctct
     4081 ccctctttga gttctggcaa ttctttgtct tcgaattttg taggccaaac ttttaaaaat
     4141 ccatcaaagt ccattgtggt tccgtttgct cttagcgtat atttcccgca tttaatatcg
     4201 attgcggttg tatcaaaaag agctggtggc aattgcgacg cgatgaatct tctccaaata
     4261 agatc
//
LOCUS       DATDNZ010000047        21155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2468015, whole genome shotgun sequence.
ACCESSION   DATDNZ010000047 DATDNZ010000000
VERSION     DATDNZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 21155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21155
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468015"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VI432_01790"
     CDS             <1..1978
                     /locus_tag="VI432_01790"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4496858.1"
                     /translation="AGKKDGFSLFGKKKDESKEIKEGTQIDPLHRDQLNQRADQVING
                     LRRIGLRAVPLQDSELLEFYYNSYNPTTIEKVGGEIKRGEAIASGEIANVVAPNSAEI
                     ASDHLKVENKFTKTIFVLGYPRYLSSGWFSPMINLPNLIDISIFIHPMNTGVALKNLR
                     KKAGQVESQLIEAQEKGLVRDPVLETAFSDIESLRDSLQQSREKLFQVGVYMTIYADS
                     SEELNKLEAEIKGVLEAKLIQIKSAVFEQLNGFASVLPLGLDKLNIHTPMNSEPLSSF
                     FPFVSLDLTSDKGILYGINRHNNTLVIFDRFSLENANMVIFAKSGSGKSYTTKLEILR
                     SLMMGTDIIVVDPENEYLQMADAVGGTVLKISLDSESNINPFDIPIIAEDENPGEVLR
                     SHIITLTGLIKLMLGKIKASEEALLDRAITETYASRDITPDRDFKGLQPPLLEDFQTI
                     LEGMEGGKEMAQRLYRFTKGSYAGFVNKPTNINIKNRLIVFSIRDLEDELRPIAMFII
                     LNFIWNLIRAELKRRIMIIDEAWWMMKYPDSAAFLYGLVKRSRKYYLGISTITQDVED
                     FLNSQYGRPIITNSSLQLLLKQSPATAEITAKAFNLTDVEKNFLLESSVGQGLFIAGL
                     KRSAIQIVPSYFEDKLITTNPEQLLKQKQSGAGE"
     gene            1993..2340
                     /locus_tag="VI432_01795"
     CDS             1993..2340
                     /locus_tag="VI432_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496859.1"
                     /translation="MADQDKKISLVEIILMLMITITADVVELILTLFVAGIVVNILIN
                     IPVWLVIQFWLIMRGIRGLSYTAGSMVEFVPIINALPLRTASMLITIYLANHPGAAKL
                     ASPKSMIKKNISE"
     gene            2342..2632
                     /locus_tag="VI432_01800"
     CDS             2342..2632
                     /locus_tag="VI432_01800"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496860.1"
                     /translation="MLSFIKYALAQNSIARDVAASAGFDLANIFQWAIGVGAILAVGV
                     MIFGGVKYTAAVGNPSIQTDAKEWIKGAVFGLLLLIGAYLILNTINPGILKF"
     gene            complement(2629..3060)
                     /locus_tag="VI432_01805"
     CDS             complement(2629..3060)
                     /locus_tag="VI432_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY4496861.1"
                     /translation="MEELKSKIELMLNLMGFNNTDVGLDVGLNKVSIKIEDEVINSSN
                     LPQFVASLNAITRLLAKKMELGPVVVDINNYRKDRERIIVDLAKEAARKAVATKESVS
                     LPAMNAYERRLVHTELSMRPDIKTESMGEGRGRYVIIKNTD"
     gene            complement(3172..3888)
                     /locus_tag="VI432_01810"
     CDS             complement(3172..3888)
                     /locus_tag="VI432_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY4496862.1"
                     /translation="MLTTLYNELLYKPLFNTLVVLYQYLTFYDLGLAIILLTIMIRIL
                     LFPLFHKTTKHQLITQALQPKIKEIQKTHKDNKETQAKAILDLYSKHKLNPFTPIGLL
                     IIQLPILIALYQVFISGFSDKAFTYLYPFISAPETLNSYFLNLIDLSKPNALLVIGAA
                     LAQYLQGRITTSKIINKTGQLSPQEKMQKNMVYFGPAVAIMILWNFPAAIGLYWLTST
                     LFSLLQQLIVSRTLDTEQFK"
     gene            complement(3869..4204)
                     /gene="rnpA"
                     /locus_tag="VI432_01815"
     CDS             complement(3869..4204)
                     /gene="rnpA"
                     /locus_tag="VI432_01815"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY4496863.1"
                     /translation="MLPKKHKFPIQFFPKNSKTVFKSYIFTIKFSPNALNYNRVGVYI
                     TTKLSKSAVSRNRIKRVVLNFFNVNKLKTLNIKNKKNIDLLIIPNTNIMKLTPKEIIK
                     EFKNAYDSL"
     gene            complement(4217..4351)
                     /gene="rpmH"
                     /locus_tag="VI432_01820"
     CDS             complement(4217..4351)
                     /gene="rpmH"
                     /locus_tag="VI432_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY4496864.1"
                     /translation="MSRTYQPKKRKRARTHGFLKRKRSTGGRRTLKRRRQKGRAKISV
                     "
     gene            4487..5857
                     /gene="dnaA"
                     /locus_tag="VI432_01825"
     CDS             4487..5857
                     /gene="dnaA"
                     /locus_tag="VI432_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY4496865.1"
                     /translation="MENEVLWENVLSELELHVSRPNFLTWLKNSRFIGLKEDNHKALV
                     ALPNHFAKEWVETKYHKLILGSIRNINNSVNKIEYVVESNLIKKGLAPVTITKIIQET
                     KQLSFQEMRVDPETNLNQRYTLNSFIVGASNELAYSAATAIVQSVGNKYNPLFIYGGV
                     GLGKTHLIQAVGNEIKEKYKNGVKVKYVTSEKFTNDVVWAIRNRRMEDVKNMYRYTDV
                     LIIDDIQFIGGKEKTEEEFFHTFNALYENNKQIIISSDRPPRSIPTLEERLRSRFEGG
                     MIVDINLPDYETRVAIIKTKLQEKLAVLEEEIIDLIAKKVQKNIREIEGILNKIIFHQ
                     TTYQKNLEANLVDKIISEMTDRRTANLNPNQVINTVANFFEISTSDITGKARNKELIE
                     PRQITVYLLRELLSMSYPYIADKIGKRDHTTAIYSYNKIVGDINKNPNLNQKILLIKD
                     ELNKLG"
     gene            6010..7122
                     /gene="dnaN"
                     /locus_tag="VI432_01830"
     CDS             6010..7122
                     /gene="dnaN"
                     /locus_tag="VI432_01830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY4496866.1"
                     /translation="MKLIILKNNLRSGLDIVGRAVGANLNLPILGNVLIKTIENKIKI
                     SATNLEIAITAFVQGKIVEDGSITVPFSVLANITSNIPNERIDIEVKNNNLILKTDNY
                     NASIQGLPESDFPIIPEIKDGAESFEINNSILKNSINKVVIAAGTNDLRPELNSVYLI
                     VEPSSLKLVSTDSFRLAEFTISGEQFKNNSERGVKIILPLKTAEALYKVIKDEDDKTK
                     FTIENNQILIESGGVGIVSRLIEGRFPDHEAIIPKEINTEVLVSRKELLNALKLASSF
                     TSRVKDIKIKIKDKKVIEIYSSDSSLGENNYLIPAKIKGENEEIVFNGKYLLDGAKSE
                     DSDQIFLGLNGDNKPTLIKTPNNTSYFYILMPIKNS"
     gene            complement(7119..9449)
                     /locus_tag="VI432_01835"
     CDS             complement(7119..9449)
                     /locus_tag="VI432_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY4496867.1"
                     /translation="MFKKIVVVVVMVFMGLFSISIVGVFWISKDIPSAEEIVNKSVAQ
                     STKIYDRTGEVLLYEIYGEEKRTIIPVEEIPDFIKYATISVEDQSFYKHPAFDIKGIL
                     RAIFIDLSRGGLIQGGSTITQQLAKNSFLTHERTIIRKVKELILAWRLEKQYTKEEIL
                     GFYLNQVPYGSNSYGVEAAAQVFFDKKAQDLDLNESALLAALPKAPSYYSPWGENIDS
                     LYNRKDFVLKQMHELGYIDDVELEENYEKIPNIKNRPETGIKAPHFVTYIQEYLNNKY
                     GEDLLRASGFKIITTLDWEMQQLAEDVIRRGAERNTELYGGKNAALIAQDPQTGQILA
                     MVGSADYFNIENEGNFNVAVKGLRQPGSAFKPFAFLTAFEKGFTPDTLVWDVETEFNT
                     TNDLEKSYKPHNFDEKFRGPITLKESLAQSINVPSVKTLYLAGIEDTIKTAEDMGITT
                     LKDRSRFGLSLVLGGGEVKLIELVGAYSVFAADGIKHDISTILEIKDMNNKIVEQYKN
                     SSRRVVDAKYVRLINDILSDTELRSPLFERSINLTRVPGHQIALKTGTTNDYIDAWTM
                     GYAPNLSVGVWVGNNHREPLTSKGGSILAAVPIWHDFISKAISKYPPQIFERQEATSS
                     ENPILRGSFPEGDPHTVLYYLNKVNDSQFNNWEDGLIEWLKNNTIDLSLFRKVDELSQ
                     NQSKINIKLSSPENGSMINNSLNLNFEIESLNKINLIEIYFNNEVIYRNSDNLGNFFS
                     YNQNVGLLSLKIQNQLTISVEDENGFRNKRDIILFH"
     gene            complement(9554..9718)
                     /locus_tag="VI432_01840"
     CDS             complement(9554..9718)
                     /locus_tag="VI432_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496868.1"
                     /translation="MDLDLNLAEDGLENLDEEETGDDDMTDDDADDDDDMTDDDANGN
                     EDEGDNEDDN"
     gene            complement(9878..10342)
                     /locus_tag="VI432_01845"
     CDS             complement(9878..10342)
                     /locus_tag="VI432_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY4496869.1"
                     /translation="MIILGIDPGSTRVGYGVIEKNGGFNLIDYGVIQEKQNGDYKNIV
                     SICDKIEKIIKKYKPVVAGLETLYFSKNKKTALSVSEARGAIILTLIKNKIGIKEFNY
                     NDVKSVVTGSGSNDKEMVRRVVAMTLGVKKIKGYDDSADALAIALRTAFEHN"
     gene            complement(10343..10864)
                     /locus_tag="VI432_01850"
     CDS             complement(10343..10864)
                     /locus_tag="VI432_01850"
                     /inference="COORDINATES: protein motif:HMM:NF045213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4496870.1"
                     /translation="MAGHSKWKQIKHKKEAADKNRSKVFAKLANAISIAAKKEPNPDF
                     NPTLRSIIERAKKENMPQENIKRALNKAKEVESLEELFVEIFGPEGSGIIIELVTDNR
                     NRTMNEVKIAAKKHEAKIALPGGLSWSFEKTESGYIPKFKQQISEEAREKIDNLIEEL
                     NSLDETQNIYLNI"
     gene            10942..12618
                     /locus_tag="VI432_01855"
     CDS             10942..12618
                     /locus_tag="VI432_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY4496871.1"
                     /translation="MNDTPKNPLEDKLKDLRSGAEERDAKRRSEELTIPYLDLRSAPI
                     SLEAFKMISEKGARLAKAAFIQIKKKNLTVCVFDPREEETARLLDKLRAEEFILEIFI
                     VSLSSLEKAWQSYRGIGSSIKEITSKIEIMGTTLDLLKEKFNSIEKLSSYLKELEEEN
                     QLFSSTTKTIELFLGSSLVLGASDLHFESQKENADLRFRIDGALHDIYKIPANLYKSL
                     LNRIKLLSGLKINISDIPQDGRFTIKASEDEIEIRVSIIPSEFGEAIVMRVLDPKAIS
                     LDLLKLGLRKDDEEIARREIKKPNGMILVTGPTGSGKTTSLYAFLKTVNDPEAKIITI
                     EDPIEYHLEGIEQTQVDQSSGYTFASGLRSILRQDPDIILVGEIRDLETAEIAIQASL
                     TGHIVFSTLHTNTAVGAVPRLIDVGIKSAVLGPALTLVIAQRLVRKLCQYCRVADERI
                     NEFKEKIDNFLTKLPPKVKSYSEEIKLYKNIGCEKCNNGFKGRIGIFEFLEFDDEMER
                     LITKDPTESAIREFAHKKGLVEMQIDGILKSLAGITTLDEVEKATGPINW"
     gene            12957..13964
                     /gene="ruvB"
                     /locus_tag="VI432_01860"
     CDS             12957..13964
                     /gene="ruvB"
                     /locus_tag="VI432_01860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY4496872.1"
                     /translation="MIKKKSQNDIKNEDLNIDSVLRPGVWDEYVGQESIKRSLKIILE
                     AARMRKEAVDHLLFYGQAGLGKTTLANLVAKEMGAQMKITTGPAIEKMGDLAAILSNL
                     EPNNILFIDEIHRLNRIVEEVLYPALESRKLHIVIGKGPGARTLSLDLPPFTLVGATT
                     RVNLLSDPLRSRFGATFRLDYYNPEDIESIIARSAKILGVKINEGAVKMLAKASRFTP
                     RVANRLLKRARDFKEVNNIGIIDEDIARRTLELLEIDELGLELHDRLILEAMIDKFNG
                     GPVGVNTLAATLNEDRDIIENVYEPYLMKLGLLRRTPSGRVVEPQAVEHLKKKITKKL
                     F"
     gene            13973..14422
                     /gene="tsaE"
                     /locus_tag="VI432_01865"
     CDS             13973..14422
                     /gene="tsaE"
                     /locus_tag="VI432_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY4496873.1"
                     /translation="MRFKSTSPRNTAFIASGIIGEILKKKRKKEAVVISLVGDLGAGK
                     TTLVKAFARAIGVKKKIISPTFLIIRRIKIFGHKKFENFFHVDAYRIKPKDIINLGFK
                     DVLKNEKNIIVIEWGDRIKKILPRNTTWINLKHSSNVLSRIIDIKKI"
     gene            14419..15297
                     /locus_tag="VI432_01870"
     CDS             14419..15297
                     /locus_tag="VI432_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY4496874.1"
                     /translation="MKTLFLIDANSLIHRAFHALPPFTSPDGRPTGALYGISNILLKI
                     LREQKPDYIAAAFDRPEPTFRDKEYREYKATRPETAEGLIPQIIGARELFSLLGIKTL
                     EMPGFEADDIIMTLANKFNQEDLRVVIFSGDLDILQAVRGENIVAQIPKTGISNTVIY
                     NKEAVQDRFGVPPEKLADFKGLVGDKSDNIPGVRGVGPKTAAVLVMQYGSLENLFQEI
                     EELGETKATKKLSGSKEIALLSKKLALLREDVPIKVDLEDFKRVNLNIPKIKEYFIQL
                     GFKTVVDRLAAGYTDN"
     gene            15306..16667
                     /locus_tag="VI432_01875"
     CDS             15306..16667
                     /locus_tag="VI432_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4496875.1"
                     /translation="MKNPNFGRPQESIADKIKNLTYLKVKEARFVWFFAPFLIAIAII
                     NIIYLPALWSFVNGILLFFLTILIGINSIKLIKYNLEVKASSLRLENIISNLKDGIIV
                     YDANFKILVFNTAAEEIFSVKKEDVLGYNFGPERAQDKRFKLLAQTIFPSLAPMVVRR
                     SEAGIFPQITDISFIDPNLELRVSTIKMSGRDGESDGFIKVVMDRTRELELYRSKSEF
                     ITVAAHQLRTPLTAVNWALELLNRNTSLSEEDISVAKDGFLASKKLLKIVDDLLDVSK
                     IEEGKFGYSFENVDLISFLEKLLENASIVAKKYGANVYFDKKGEKSMVVTADPNRINT
                     ALSNIIDNAIKYNTKNGSVTVRVERMKDKPFVEIVIEDTGIGIPAEDIPQLFKKFFRS
                     DNAVKLETEGSGLGLYITKNIIMRHGGTIWAESTIGRGTSFHINLPTDPKLIPAKEVV
                     YGE"
     gene            16736..17512
                     /locus_tag="VI432_01880"
     CDS             16736..17512
                     /locus_tag="VI432_01880"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496876.1"
                     /translation="MTILLYGPNSYLRLKKSNEIVDFYKKQKEGFNLDGFDLSDKEQF
                     INFEERIRSSSMFSNNKFFIIQNVFDSDDKKELKKILKAHLEDEENVILMNSDIKPPA
                     DFKFLITKPAKSELFDNLKSDTEIIDFIKKEAEDRGVKLEDKDFFSLRQAFDNDLWAI
                     TTELDRLSLSESVRIEKKEKYEYFQILNALKYGFDARKKLIALEIILSDRRDEPARVF
                     NSLSYGLKDQISINFFADLDIAVKSGKLEYEEVLLSFALI"
     gene            complement(17505..17765)
                     /gene="rpsT"
                     /locus_tag="VI432_01885"
     CDS             complement(17505..17765)
                     /gene="rpsT"
                     /locus_tag="VI432_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY4496877.1"
                     /translation="MPITKSAEKALRQNIRRREMNLNRKKKLKSTIKDYQKSVSSNNI
                     DDAKTKLPAVYQALDKAAKKGLIKKNKANRLKSRLTARLKTK"
     gene            complement(18264..18725)
                     /locus_tag="VI432_01890"
     CDS             complement(18264..18725)
                     /locus_tag="VI432_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496878.1"
                     /translation="MAAGHQDYKEGQNATTIIRRVKVNRTLTPEQVIEATGRTQYVNP
                     DVLATMPQGEGEEVEVHFFNLGRDLTQDELECEYERLGLVPDPRAQAQVNTEDPAFAD
                     DHPNATQWDVQDNVSSFVTFVRWSDKRIVGVDRGGKDWSSYGWFAGVHKSR"
     gene            18843..20234
                     /gene="cysS"
                     /locus_tag="VI432_01895"
     CDS             18843..20234
                     /gene="cysS"
                     /locus_tag="VI432_01895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4496879.1"
                     /translation="MLIYDTLSGEKKKLEQTKDRINLFVCGPTVYDYSHIGHARTYIF
                     FDLFVKYLRSLGYKIFYLQNITDIDDRIIVRAKERSTEVQKLAKEFTDKYLEDMKALG
                     VTEVDKYAPATKFIKQIVGQVKTLIDKGYAYKTSDGYYFDISKFEDYGKLSRRSAEQA
                     ESAVSRIDESVTKNNKGDFALWKFYKEGEPFWETELGKGRPGWHIEDTAITEKYFGPQ
                     YDIHGGGIDLKFPHHEAELAQQESASGKSPFVKIWMHSGFLLADGKKMSKSLNNFITI
                     GDFLTKHDPLVLKWIVLTHHYRSPVNYTESLISQSISAINGLRESLAKLGFASNKSNK
                     DSSFDLQEKLKNVVIEFESALSDDFNTPLAIAVILSFLGDINDKIWNLSKIEAKSILK
                     IIKNTLSVFSIELKADAIPLNIKKFIDEREEFRRNKQFIQSDALRKKTEDLGYIIEDT
                     PFGPFIIKTNAIN"
     gene            20221..>21155
                     /locus_tag="VI432_01900"
     CDS             20221..>21155
                     /locus_tag="VI432_01900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY4496880.1"
                     /translation="MQSIKDLKVQPQDLDAEQSVLGALMIDKNAIFQIADVLNPGDFY
                     KRSHSIIFEAILQLWQRHEPIDILSVTAELKKMDKLKDAGGSSYLTEIINSVPTASHV
                     AHYAKIVKEKRILRELINASAEITEDAFNPDDDLETLLDKIEQRVFSISQKSIAKNFV
                     PLKEELKGAYERIEKLHQGGGAGIRGVPSGFIGLDNLLSGFQKSDLIIVGARPSYGKT
                     AFVLDIARQAALSGTTVAFFSLEMSREQVVDRLISSEAQVDLWKLRTGRLKDESEFAM
                     IQNALGKLNNAPFFIDDTPSPNVVQMRSMARRLQA"
BASE COUNT         6888 a         3648 c         4081 g         6538 t
ORIGIN      
        1 agccggaaaa aaagacggct tttctctgtt cggtaagaaa aaagatgaat caaaagaaat
       61 taaagagggt acacaaatcg accccctgca cagagatcaa cttaatcaac gggctgatca
      121 ggttataaac ggtctaagac gcataggact aagggctgtg ccgcttcaag actctgaatt
      181 attggaattt tattacaatt catataaccc taccactatt gaaaaggtcg gcggggagat
      241 taaaagaggc gaagcaatcg caagcggaga aatagcaaat gtagtcgcgc ctaactcggc
      301 tgaaatagca tccgaccatc ttaaggtgga aaataaattt actaaaacaa tttttgttct
      361 tggttatcct agatatctgt caagtgggtg gttctcgcca atgatcaatc tgcctaattt
      421 aatagatatt tctattttta tacacccgat gaacaccggc gtagcactta aaaatctgcg
      481 taaaaaagcg gggcaggttg aatcacaatt aattgaagct caagaaaaag gtttggttcg
      541 cgatccagtc ttagaaaccg catttagcga tatcgagtcg cttagagact cccttcaaca
      601 atccagagaa aaactttttc aagtcggcgt ctatatgact atctatgccg attcaagtga
      661 agaattaaat aaattagagg ctgagataaa aggagtgttg gaagcaaaat taatccagat
      721 taaatcggct gtttttgagc aattgaatgg atttgcctct gttcttcctt tgggattgga
      781 taaattaaat atccacacgc ctatgaactc tgagcctctt tcatccttct ttccgtttgt
      841 atctttagac ctaacttcag ataagggaat cctctatgga attaaccgcc acaacaacac
      901 gcttgttatt tttgatcggt tctcgctcga aaacgccaat atggttattt tcgctaaatc
      961 aggatccggt aaaagctata ccaccaaatt agaaattttg agatctctta tgatggggac
     1021 agatattata gttgtcgatc ctgaaaatga atatttgcag atggcggatg ctgtcggagg
     1081 aactgtttta aaaatttcct tagattcaga aagtaatatt aacccttttg atatcccaat
     1141 tatagccgaa gacgaaaacc ctggggaagt attgcgttcc catattataa ccttgactgg
     1201 tcttataaaa ttaatgcttg gtaaaataaa agcctccgaa gaagctctac tcgatagggc
     1261 catcaccgaa acatatgcct caagagacat aacgccagac agagatttta aaggattgca
     1321 accccccctc ttagaagatt ttcagactat tttagaagga atggagggtg gaaaagaaat
     1381 ggcccagaga ttatatagat tcactaaagg cagttatgct ggatttgtta ataagccgac
     1441 caatatcaac ataaaaaaca gattaatagt tttttcaatt agagaccttg aagacgaatt
     1501 gagaccgatt gctatgttta ttattttaaa ctttatttgg aatttgatac gggctgaatt
     1561 gaaaagaaga attatgatta ttgacgaggc ttggtggatg atgaaatatc ctgacagcgc
     1621 agccttttta tatggactag tgaaaagatc aagaaaatat tatttgggaa tatcaaccat
     1681 cacccaagat gttgaagatt ttctaaattc acaatatgga agaccgatta taaccaactc
     1741 ttctcttcag ctacttttaa aacaatcacc ggcaacagca gagataacgg caaaagcttt
     1801 taacctgacc gatgtagaaa agaatttttt attagaatct tctgtcggac aagggttatt
     1861 tatcgctgga cttaaaaggt cagcaattca aatcgtgcct tcttattttg aagataaact
     1921 aataaccact aatccggaac aattattaaa acaaaaacag tcgggcgccg gagaataaaa
     1981 catatttaga caatggccga ccaagataaa aaaatttctt tagtggagat tattctaatg
     2041 ttaatgatca ctataacagc agacgtagtt gagctcatat taaccctttt tgtggcagga
     2101 attgttgtta atatattgat taatattcct gtgtggctgg tcatccaatt ctggcttata
     2161 atgaggggta taagaggatt atcatataca gcaggcagca tggtagaatt tgttccgatt
     2221 attaatgctc tccctcttag gaccgcatca atgctaataa ctatttatct cgctaatcat
     2281 cctggggcgg caaaactagc ttctccgaaa tcaatgatta aaaagaatat atcagaataa
     2341 aatgttaagt tttataaaat acgcattagc tcagaacagt atcgcgcgag atgtcgccgc
     2401 gtcagcggga ttcgatttag cgaatatatt ccaatgggca ataggggttg gcgcaatact
     2461 agctgtcggg gttatgattt ttggtggcgt aaaatacacc gccgcagtgg gtaatccatc
     2521 tatccaaaca gacgctaaag aatggatcaa aggagctgtc tttgggttgc ttcttttaat
     2581 cggcgcttat cttattttaa acacaattaa tcccggtatc ttaaagtttt agtctgtgtt
     2641 tttaattatc acatatctac ccctgccctc tcccatactc tcagttttaa tatctggtcg
     2701 catactaagt tctgtatgca caagccttct ttcgtaagca ttcatcgctg gaagagaaac
     2761 cgactctttt gtcgcaaccg cttttctagc ggcttcttta gctagatcca ctattattct
     2821 ttctctatct ttcctatagt tattaatatc caccacaacc gggccaagct ccattttttt
     2881 agctaaaagg cgtgtaatcg cgtttaaact agccacaaac tggggcaaat tcgagctatt
     2941 gataacttca tcctcaattt ttatagaaac cttattaaga ccgacatcta aaccaacatc
     3001 agtgttatta aaacccatta aattaagcat gagttctatt ttagatttta attcttccat
     3061 aatattatcg gtttattgta tggcatttaa tctctcttgt cacgaccgta accatgcatt
     3121 aaacgaaaac caaaaccaat aataacctct ctaaactaag aatatcaagt tttatttaaa
     3181 ttgttccgta tctaaagtcc tactaactat caactgctgt agcaatgaaa ataaagttga
     3241 tgtaagccaa tacagcccga tcgccgcagg aaaattccac agaatcataa tagctacagc
     3301 cggcccgaaa taaaccatgt ttttttgcat tttttcttga ggtgataatt gcccggtttt
     3361 attaataatt ttacttgtcg taatcctacc ctgcagatat tgagcgaggg ccgcgcctat
     3421 taccaataaa gcattaggct tacttaaatc tattagattt aagaaataac tattcaaagt
     3481 ttcgggcgca gagataaaag gatataaata agtaaatgct ttatctgaaa atccgcttat
     3541 aaaaacctgg tataaagcaa taagaatagg tagttggatt ataagcagcc cgatcggggt
     3601 gaacggattc agtttatgtt ttgaatataa atcaagaatc gctttagctt gcgtctcctt
     3661 gttgtctttg tgggttttct gaatctcttt tatttttggt tgtagcgctt gggtgattaa
     3721 ttgatgtttt gttgttttgt ggaaaagagg aaataataaa atccttatca ttatagtaag
     3781 aagaatgatg gctaggccca aatcgtagaa agttaaatac tgatacagaa cgactaaagt
     3841 gttgaatagg ggtttatata ataattcgtt ataaagagtc gtaagcattt ttaaattctt
     3901 taattatttc ttttggggta agcttcataa tattcgtatt aggtattata agcaaatcaa
     3961 tatttttctt gtttttaata tttaacgttt ttaatttatt cacattaaaa aaatttaaaa
     4021 caactctctt aatacggttt ctcgagactg ccgacttgct gagttttgtg gttatataaa
     4081 caccaaccct attatagtta agcgcgtttg gtgaaaattt gattgtaaaa atataagatt
     4141 tgaaaacggt tttagagttc ttaggaaaga attggatagg aaatttatgt tttttaggaa
     4201 gcataaacta tgccctttaa acagaaatct tggctctgcc tttttgtctt cgccttttca
     4261 gcgtcctcct gccgccagtg cttctttttc ttttaaggaa gccgtgcgtt ctggctcttt
     4321 tccttttttt aggttggtat gttctagaca tatctaacaa tcctaatatc ataattgtct
     4381 tttttcaagc ttttgaaaca aatttgagat atccacaaga aatcaacagt ttattcaaag
     4441 acaaaatatt tgactgctgc gtttaattaa ataagataag acatatatgg agaatgaagt
     4501 gttgtgggaa aacgtgctct cggaattaga gttgcacgta tctagaccta actttctaac
     4561 ctggttgaag aacagtaggt ttataggtct taaagaagac aaccataaag cgcttgtcgc
     4621 tctgcccaat cattttgcaa aggaatgggt tgagactaaa tatcataaac ttattctcgg
     4681 atcaatacgc aatattaata attctgttaa taaaattgaa tacgttgttg aaagtaattt
     4741 aattaaaaag gggttggcgc cggttaccat aactaaaata attcaagaaa caaaacaatt
     4801 aagttttcaa gaaatgagag ttgaccccga aacgaacctt aatcaacgtt atacgcttaa
     4861 ttcgtttatt gttggggcgt caaacgaatt agcatattca gcggctacag ctatagtgca
     4921 aagtgtcggc aataaatata atccgctttt tatatatgga ggcgttggtt tgggcaagac
     4981 acatttaata caagctgtag gcaacgagat aaaagaaaag tataaaaacg gcgtcaaagt
     5041 aaaatatgtt acatcagaaa agtttacgaa tgatgttgtt tgggcaatta gaaaccgtcg
     5101 gatggaagat gttaaaaaca tgtatagata taccgatgtt ttaattattg atgacataca
     5161 gtttattggg ggcaaagaga aaacggagga agaatttttt cacacattta acgcattgta
     5221 tgaaaacaat aagcaaatta ttatctcttc ggataggccc ccacgatcaa taccaacctt
     5281 agaagaaaga ttaagaagta ggttcgaggg ggggatgata gttgatatca acctgcctga
     5341 ttatgaaacg cgcgttgcta ttattaaaac aaaactacaa gaaaaattag ctgttttaga
     5401 ggaggaaata atagatttga ttgctaaaaa ggtccagaaa aacatcagag agattgaggg
     5461 aatattaaat aaaattatat ttcatcagac aacttatcaa aaaaatttgg aggccaatct
     5521 tgttgataaa ataatatcag agatgacaga cagaagaacc gcgaacctaa acccaaacca
     5581 agtaataaac actgtcgcta atttttttga aatctcaaca agcgacataa caggcaaagc
     5641 aaggaataaa gaattaattg aacctcgcca gataactgtt tatcttttaa gagagcttct
     5701 tagtatgtct tatccttata tcgccgataa gataggcaaa agagatcaca ccacagcaat
     5761 atattcctac aataaaatag tcggcgacat caacaaaaac ccaaatctaa atcaaaaaat
     5821 tttattaata aaagatgagt tgaataaatt ggggtaaagg tgtttataaa ttatggataa
     5881 ttttatacaa caaaataaaa tatacagatt atccacaaaa tttaacaacc acaaagagcc
     5941 ataaaaccaa ctaaacaaaa caaaaaaaca acttatcaac agaactaaca accataacta
     6001 aaactatata tgaaattaat aatattaaag aataatttaa gaagtggatt ggatattgtg
     6061 ggtagagctg ttggagcgaa tctaaaccta ccaattttag gcaatgtttt aataaaaacg
     6121 attgaaaaca aaattaaaat atcggcaact aatttagaaa tagcgattac agcctttgtt
     6181 caaggaaaaa ttgttgaaga cgggagtata accgttccgt tttctgtttt ggctaatata
     6241 acttcaaaca ttccaaacga gaggatagat atagaggtta aaaacaacaa ccttattctt
     6301 aaaacagata attataatgc ttcgatccaa gggttacccg agtctgattt tccaataatt
     6361 ccagagataa aagacggagc cgaatctttt gaaataaata atagtattct aaaaaactca
     6421 atcaacaaag ttgttattgc tgcaggcaca aacgatttaa gaccagaact aaacagcgtt
     6481 tatttaattg ttgaaccctc atctcttaaa ctggtttcaa cagacagttt tagattggca
     6541 gaatttacta taagcggaga acagtttaaa aataattctg agcgaggagt aaaaataata
     6601 ttacccctta aaacagccga ggcgttgtat aaggttatta aagacgagga tgataaaaca
     6661 aaatttacaa ttgaaaataa ccaaatatta atagagtcgg gtggcgttgg gattgtttca
     6721 aggttgatag aggggagatt tccagatcac gaagctatca tcccaaaaga aataaataca
     6781 gaagttttgg ttagtaggaa ggaattatta aacgctctta aattagccag ttcgtttaca
     6841 tcaagagtaa aagatataaa aataaaaatc aaagataaaa aagttattga aatttattca
     6901 agcgactcct ctcttggcga gaacaactat cttataccag cgaagattaa aggagaaaac
     6961 gaagaaatag tttttaacgg taagtatttg ttagatggag caaagtccga ggattcagat
     7021 cagatatttt taggtttgaa tggggataat aagccaaccc ttataaaaac acccaacaat
     7081 acgtcatatt tttatatact gatgcccatt aagaactctt aatggaacaa aattatatct
     7141 cttttatttc tgaaaccatt ttcatcctct actgaaattg tgagttggtt ctgtattttt
     7201 aaacttaaca aaccaacatt ttgattataa gaaaaaaaat ttcccaaatt atctgagttt
     7261 cgatatataa cttcattgtt gaaataaatt tctatcaagt ttattttatt cagggattct
     7321 atttcgaagt taagattaag agaattgtta atcatgcttc cgttctctgg tgaagacaat
     7381 ttaatattta ttttactttg gttttggctt aactcatcaa cctttctgaa taaagacaga
     7441 tcgatcgtat tatttttcag ccactcaatt aaaccatcct cccaattatt aaactgcgag
     7501 tcattaactt tatttagata gtatagtacc gtatgaggat cgccctcagg gaaactacct
     7561 cttaatatcg ggttctcgga agatgttgct tcttgtctct caaaaatttg aggtggatat
     7621 ttagagatcg ctttagatat aaaatcgtgc cagatcggaa cagcggcaag aatgctgccc
     7681 cccttacttg ttagaggctc tcgatggttg ttgccgaccc aaacaccaac cgatagattt
     7741 ggtgcataac ccattgtcca agcgtcgata taatcattgg ttgttcctgt tttaagagct
     7801 atttgatgac caggaactct agttaaattg atgcttctct caaacaaagg cgatcttaat
     7861 tctgtgtcag ataatatatc atttattaat cttacatatt ttgcatcaac aacccttctg
     7921 ctagaatttt tatattgctc aactatttta ttattcatat ccttaatctc taaaatagtt
     7981 gaaatatcgt gttttatccc atctgccgcg aatacagaat aagctccgac taattctatt
     8041 aatttaacct ccccgcctcc caaaactaaa gacaatccaa atctagacct atctttaagt
     8101 gttgtgattc ccatatcctc agcggttttt atagtgtctt caatcccagc taaatataaa
     8161 gtcttaaccg atgggacgtt tattgattga gctaaggact cttttaatgt tatcggaccc
     8221 ctgaattttt catcaaaatt atgcggtttg taactttttt ccaaatcgtt ggttgtattg
     8281 aattctgtct ctacatccca aaccaaagtg tctggagtaa aacccttttc aaaagcagtc
     8341 aaaaaagcga atggtttgaa tgctgatccc ggctgtctca accccttaac cgcgacatta
     8401 aaattgccct cgttttcaat attgaaatag tcagcagacc cgaccatcgc taggatttgt
     8461 ccagtttgcg ggtcttgggc tattagcgcc gcattttttc caccatacaa ttctgtattt
     8521 ctttcagcgc ctcgcctgat cacatcttct gctaattgtt gcatttccca atccaacgtt
     8581 gtgataattt taaaaccgga ggctcttaat aaatcctctc cgtatttatt atttaaatat
     8641 tcttggatat aagttacgaa gtgtggcgct tttattcctg tctcgggccg gttttttatg
     8701 tttggtattt tttcgtaatt ttcttccaac tccacatcgt ctatatatcc caactcatgc
     8761 atctgcttta aaacaaaatc ctttctattg tataaactat cgatattttc tccccatggg
     8821 gagtagtaac ttggcgcttt aggcaaggcg gccaaaagag cagattcgtt taaatctaaa
     8881 tcttgagctt ttttatcaaa aaaaacttgt gcagctgctt ctactccata agaatttgaa
     8941 ccgtaaggaa cttgatttaa gtaaaagcct aaaatttctt cttttgtgta ttgtttttcc
     9001 aatcgccaag cgagtatcaa ttctttaacc tttcttatga ttgttctctc gtgcgttaaa
     9061 aaagaatttt ttgctaattg ttgcgttatt gtcgagccac cctgtatcaa tccgcctcga
     9121 gacaaatcta taaaaatagc tcgcaatatg ccttttatgt caaaagcggg gtgtttataa
     9181 aaactctgat cttctacgct tatagttgca tattttataa aatcaggaat ttcttcaacg
     9241 ggtatgattg ttcttttctc ttctccatat atctcataca aaagaacctc cccagtcctg
     9301 tcatatattt tagttgattg cgcaaccgat ttattgacta tctcttctgc agatgggata
     9361 tctttactaa tccaaaagac tccaactata gatatagaaa acaaccccat aaacaccatc
     9421 acgaccacca ctactatttt tttaaacatg aatattacta aaactttatt ctaaaaataa
     9481 ccctaacaca acaaaacccc gctgacgcca atcatttcag aaataataag caaaatagcg
     9541 gggtttagtt tgtttagttg tcgtcctcgt tatctccctc gtcttcattg ccattcgcgt
     9601 cgtcatccgt catatcatcg tcgtcatctg cgtcgtcatc cgtcatatca tcgtcgccag
     9661 tctcttcttc gtcgaggttt tccagaccat cttcggcaag attaagatct agatccatta
     9721 ttgtttgcat tttattgttt ttttcgaccg ttttagttag cagtagtttt ttgtttataa
     9781 acgctttcta agcgcaaatt ttaagagaaa gtatatacaa taattttatg gtgtcaatgc
     9841 ctatgtcaaa cattgattac tcatagcgca aacgtgttta attatgctca aaagctgttc
     9901 gtagtgctat agcgagggcg tctgccgaat catcgtaacc cttaattttt tttacaccca
     9961 aagtcatagc tacaactctt ctgaccatct ctttatcatt gctgccgctt ccagtaacga
    10021 cacttttaac atcattataa ttgaactctt ttatgcctat cttatttttg ataagagtca
    10081 atattatcgc tccccttgct tctgaaacag acagagctgt ttttttattt tttgaaaaat
    10141 ataaagtctc taaaccagcg acaaccggtt tatatttttt aataattttt tctattttat
    10201 cgcaaataga gacaatattt ttataatcgc cgttctgctt ctcttgtatc acaccataat
    10261 ctatcagatt aaatcctcca tttttttcga taactccata accaaccctt gtactgcccg
    10321 gatctattcc tagaattatc atttaaatat ttagatatat attttgtgtt tcgtctagag
    10381 agtttaactc ctctattaaa ttatcaatct tttctctagc ttcttctgag atttgttgtt
    10441 tgaatttagg aatgtaaccc gactctgttt tttcaaagct ccatgataat cctccaggta
    10501 gagcaatttt cgcttcgtgt ttttttgcgg ctattttaac ttcattcata gttctattcc
    10561 tattatcagt aacaagctct attattatcc cgcttccttc cgggccgaag atctcaacaa
    10621 aaagctcttc taagctttcc acctcttttg ctttattaag agctcttttg atgttttctt
    10681 gtggcatgtt ctctttttta gctctttcaa taatacttct aagggtaggg ttaaaatccg
    10741 ggtttggttc ttttttagcc gctatagata tggcgttggc aagtttagca aataccttgc
    10801 tcctgttttt gtcagccgct tcttttttgt gttttatttg tttccatttc gagtggccag
    10861 ccattttatt atttgttagt ggttagtaat tagagcttcg gcaattgagt aattgcgcct
    10921 ttatttatat catattaaga aatgaatgac acacctaaaa atcccctaga agacaaatta
    10981 aaagatttaa ggtctggagc agaagaaagg gacgctaagc gcaggagcga agaactgaca
    11041 attccctact tagatttgag atcagccccg attagtttag aagcttttaa aatgataagc
    11101 gagaaaggcg caagattggc taaagcggct tttattcaaa taaaaaagaa aaaccttaca
    11161 gtttgcgttt ttgatccgag agaagaagaa acagcaaggt tgcttgataa attgagggca
    11221 gaagaattta ttttagagat atttattgtc tctttgtcgt cgcttgagaa ggcgtggcag
    11281 agttataggg gtataggatc ttcgataaaa gaaataactt caaaaataga gataatgggg
    11341 acgacgctag atctattaaa agaaaaattc aacagcattg aaaaattatc ttcatatctc
    11401 aaagaattgg aggaagaaaa tcaacttttt tcttctacaa ctaaaacaat cgaattgttt
    11461 ctaggttcat ctttggtttt gggagcttca gatttgcact ttgagagtca aaaagaaaac
    11521 gccgatctaa gattcagaat agatggagcg cttcatgata tttataaaat accagcaaat
    11581 ttatataagt ctcttttgaa caggataaaa cttttatccg gtttgaaaat caatatttca
    11641 gatattcctc aagatgggag attcaccatc aaagctagcg aagacgaaat cgagattagg
    11701 gtttctatta ttccttcgga gttcggtgag gcgatagtta tgcgtgtttt agatccaaaa
    11761 gcaatatcat tagacttgct aaaactcgga ctaagaaaag atgacgaaga aattgccagg
    11821 cgcgagatta agaagccgaa cggcatgatc cttgtaactg gtccaaccgg aagcggaaaa
    11881 acaaccagtt tatacgcctt tttaaaaact gttaacgatc cagaggctaa gattattacc
    11941 attgaagatc ccatagaata tcatcttgag gggatagagc agactcaagt tgatcaatct
    12001 agcggctaca cgtttgcttc tggtttgagg tctattttgc gacaagatcc agatattatt
    12061 ctagtcggag agatcaggga tttggaaaca gccgaaatag cgatccaagc ttctcttacg
    12121 ggacacatag ttttttcaac gcttcacaca aatactgccg tcggggctgt tccgagactt
    12181 atagatgttg gcattaaaag cgcggtttta ggtccggcgt taactcttgt gatagctcaa
    12241 aggttggtta ggaagctttg tcaatattgt agggttgccg atgaaagaat aaatgaattt
    12301 aaagaaaaaa tagacaattt tttgactaaa cttccaccta aagttaaatc atatagtgaa
    12361 gaaattaagc tatataaaaa tataggctgt gagaagtgta ataatggatt taaaggtagg
    12421 atagggattt ttgaattctt agagtttgat gatgagatgg aaagattaat aaccaaagac
    12481 ccgactgaat ctgcgattag agaatttgct cacaagaagg gtttggtgga aatgcagata
    12541 gatggtatat tgaaatcact ggctggcatc actaccttag atgaggttga gaaagccacc
    12601 ggcccgatta attggtaaat aaaaaagata taggtctgcg ggcaattcct tgaggggaag
    12661 cgtgctctca tgataaaaat aatcaaaaga gttttgagtt tccttgagga gtagcccagc
    12721 ctgaaccaaa ctatcctgaa tctgaagaaa aaactcgctg cccgcaggcc tatatctttt
    12781 cagtgaataa aaatacctgg ttctcaggtt atttttaatt caattatagc ttaacatagt
    12841 aaattaaaat tgtcaatact taggctgaat atactagtaa atacgttatt tttaaggttt
    12901 ttgctcaata gcaaagctat aaactctatg ttagagtatt acaaactacg caaaagatga
    12961 ttaagaaaaa atcccaaaac gacatcaaga atgaagattt aaatattgat tctgttcttc
    13021 gtcccggcgt gtgggatgag tatgtcggcc aagaaagcat aaaaagaagc ctaaaaataa
    13081 ttttagaagc ggctcgtatg cgtaaagagg ccgttgatca tttattgttt tatggccagg
    13141 ccgggttagg gaaaacaacc ctcgctaatt tggttgctaa agagatgggt gcgcaaatga
    13201 aaattacaac cggacccgca atagagaaga tgggtgatct ggccgcaatt ttgagcaact
    13261 tagagccgaa taatattttg tttattgatg aaatccaccg cttaaacaga atagttgaag
    13321 aggttttgta tccggcgctt gagtcaagaa aattacatat tgttatcgga aaaggtccag
    13381 gagctagaac tttatcttta gacctgcctc catttactct tgttggagcg acaacaagag
    13441 taaatctttt atccgacccg ttgcgttcca ggttcggcgc cacctttagg cttgattatt
    13501 ataatccgga agacatagaa tcaattattg caagatcggc aaagattttg ggggtaaaaa
    13561 taaatgaagg agctgttaag atgcttgcga aggcaagtag atttactcct agggtggcta
    13621 atcgtctttt gaagagggct agagatttca aagaagttaa taacatcgga attatagatg
    13681 aagatatcgc tcggaggaca ctggaattgt tagagattga tgaattaggt ttggagctgc
    13741 atgataggct gattttagaa gcaatgattg ataaatttaa tggcggtccg gttggagtaa
    13801 atactctggc ggctacccta aacgaggata gggatatcat cgaaaatgta tatgagccct
    13861 atcttatgaa gttgggtttg ttgaggagga ctccatcggg aagggttgta gagcctcaag
    13921 cagtagaaca tcttaagaaa aaaataacta aaaaattatt ctaagaggat aaatgagatt
    13981 taaatctact agtccgcgga ataccgcttt tattgcaagc ggaattatcg gcgagattct
    14041 taaaaaaaaa cgcaaaaaag aagccgttgt aataagcctt gtgggagatt tgggggctgg
    14101 caagacgacc ctagtgaagg cttttgcgcg cgcaataggc gttaagaaga aaatcatatc
    14161 cccaaccttt ctaataatta gaaggattaa aatctttgga cataaaaaat ttgaaaattt
    14221 ttttcatgtg gatgcctata ggataaagcc gaaagatatt attaacctag gatttaaaga
    14281 cgtgcttaaa aatgaaaaaa atattattgt tatagaatgg ggagatagaa ttaaaaaaat
    14341 attaccgcga aacacgacat ggataaatct aaaacacagc tctaatgtac tttcaagaat
    14401 tatcgatatt aaaaaaatat gaaaacttta ttcctaattg atgctaactc tttaatacac
    14461 agagcgtttc atgctcttcc gccatttact tctcccgatg gacgcccaac aggcgcgtta
    14521 tatggaatat caaatattct gcttaaaata ttacgagagc aaaaaccaga ttatatagcc
    14581 gcagcctttg ataggcccga acccactttt agagacaagg aatatagaga atataaagcg
    14641 acgaggccgg aaacagcaga ggggttaatt ccccaaataa taggcgcgag agaattattt
    14701 tctctgttgg gtattaaaac tttagagatg cctggttttg aggcagacga tattattatg
    14761 actttagcca ataaattcaa tcaagaagat ttaagggttg ttattttttc cggagatttg
    14821 gatattttac aggctgtaag aggggaaaat attgtcgccc agatccctaa aactggcatt
    14881 agtaatactg ttatttataa caaagaagct gttcaagatc gttttggcgt gccaccagag
    14941 aaattagccg actttaaagg tctggttggc gacaaatccg ataatattcc cggtgttcgc
    15001 ggtgtaggcc ctaagaccgc cgcagtcttg gttatgcagt atggatcgct tgagaattta
    15061 ttccaagaaa tcgaagaatt aggagagaca aaagccacta aaaaattatc agggagcaaa
    15121 gagatagcct tactgtctaa aaaactagcc ttattaagag aagatgtgcc gataaaagta
    15181 gacctggaag attttaaaag agttaatctt aatattccaa aaattaaaga atattttatc
    15241 caactcgggt ttaaaactgt ggttgatcgg cttgcggcag gttatactga taactgactt
    15301 atattatgaa aaatcccaat ttcggaagac cgcaggaatc aatagcagat aaaatcaaaa
    15361 acctgactta tctaaaagtt aaagaagcaa ggtttgtttg gttttttgcg ccatttttga
    15421 ttgcgatcgc gattataaat atcatttatc ttcccgcttt gtggtctttt gtaaatggga
    15481 tcctgttgtt ttttctaaca attttaattg ggataaacag cattaaactt attaaatata
    15541 atttagaggt taaagcttca tctttgaggt tggaaaatat tattagcaat cttaaggatg
    15601 ggattatagt ctatgatgca aatttcaaga ttttagtttt caatacggca gctgaagaaa
    15661 ttttttcagt taaaaaagag gatgtgcttg gatataactt cggtccagaa agagctcaag
    15721 ataaaaggtt taaattatta gctcagacta tttttccttc tctcgcgcct atggttgtca
    15781 gaaggtcgga agcaggaata tttcctcaga taacagacat ttcttttatc gatcctaatt
    15841 tagagttaag ggtgtctact attaagatgt ccgggagaga tggcgaatca gatggattta
    15901 ttaaggttgt aatggataga acccgcgaat tagaacttta tagaagcaaa tccgagttta
    15961 taacagttgc ggcccatcaa ttgcgcacgc ccctaaccgc tgttaattgg gcgcttgaac
    16021 ttctgaatag aaatacgtct ttgagcgaag aggatataag cgttgcgaaa gacggatttt
    16081 tagcatccaa gaagctttta aaaatcgtgg atgatctatt agatgtttct aaaattgaag
    16141 agggaaaatt tggatatagt tttgaaaacg ttgatttgat ctctttcttg gagaaacttt
    16201 tagagaatgc aagcatcgtt gctaaaaagt atggagcgaa tgtttatttt gataaaaaag
    16261 gagagaaatc tatggttgta acagccgatc ccaatagaat taatactgcg ttatctaata
    16321 ttatcgacaa cgcgattaaa tataatacta aaaatgggag cgtaactgtg cgcgtagagc
    16381 gtatgaaaga taagcccttc gttgagatcg taatcgagga tacaggaata ggtatcccag
    16441 ccgaagacat tccgcaatta ttcaaaaaat tttttagatc tgataatgcg gttaagcttg
    16501 agaccgaagg atctggttta ggtttgtata tcactaaaaa tataattatg cgccacggtg
    16561 ggacaatttg ggcggaatct accataggcc gcgggacatc ttttcatatt aatctgccga
    16621 ctgaccccaa gctcatacca gcaaaagaag tagtctacgg agaataggcg ttttaaatat
    16681 ctaataaaga agattagaat taattaggga aaaggatatt atccccctaa ataaaatgac
    16741 gattctatta tacggcccta attcgtattt gagacttaaa aagtcgaacg agattgttga
    16801 tttttataaa aaacaaaaag aaggatttaa tttagacggt tttgatctct ctgataaaga
    16861 acagtttatt aattttgaag aaagaatcag gagctcgtca atgttttcaa ataataaatt
    16921 ttttataatc caaaacgttt tcgattcaga tgataaaaaa gaattaaaaa agatattgaa
    16981 ggcgcattta gaggatgaag aaaatgttat tctgatgaat tctgatatca agcctcctgc
    17041 ggattttaaa tttcttatta caaaaccggc taaaagtgaa ttattcgaca acttaaagtc
    17101 tgatacggag attatcgatt ttataaaaaa agaggcggag gaccgaggag taaaattgga
    17161 agataaggat ttcttttctt tgcgccaagc ttttgataat gatctttggg cgataactac
    17221 ggagcttgat aggttgtctt tgtctgaaag cgttcgaata gaaaaaaaag aaaaatacga
    17281 atactttcag atattaaacg ctttaaaata cgggtttgat gcgagaaaaa aattgatagc
    17341 gcttgagata attcttagcg accggagaga cgagccagct agagttttta acagtttaag
    17401 ttatggattg aaggatcaga tatcaataaa tttttttgcc gatttggata ttgctgttaa
    17461 atccggaaaa ttggaatacg aagaggtgtt attaagcttt gctcttattt agtctttaat
    17521 ctcgctgtaa ggcgggattt tagtctgttc gctttatttt tcttaattaa gcccttctta
    17581 gcagctttat ccagtgcctg atacacggca ggcagttttg ttttggcgtc gtctatattg
    17641 tttgacgaaa ctgatttttg ataatcttta atggtcgatt ttaatttttt cttgcgatta
    17701 agattcatct ctctccttcg aatattttgt cgcaacgcct tttcggctga ttttgttatc
    17761 ggcatatttg gtagttaata gtaaggcgga tcatataaat tgtaaaggga gttaatttat
    17821 gattagtcgt ttacgggttt gagtagaaag cttttgagag cgcgctccag gccgcttagg
    17881 ctgagccttt tactctcaca atattattta gatgattatt taataaacaa aacaagtttt
    17941 tgtggtatat ccccttttta tccccatggg gataaaaagg ggatgaagat tttgtgtcga
    18001 atatattggt tggaaaagct tattagcaaa atagctaatc aaccaatcgg cgatacagaa
    18061 gataattaat aagagacctt gatttaattc ggggtgtttt taatttgacc ctgaaacaaa
    18121 aaagcccgtg tcggatgact cgggaagcac ttcgacacgt gctcagtgtg aaagatttta
    18181 tagttgaatc tctcgaaact tttgggagag gatggggaaa gaacaaaaac agagtgttgc
    18241 ttatcccgtt agaagaaact cttctaacgg gatttgtgaa cgccggcgaa ccacccgtag
    18301 ctgctccagt ctttgccgcc gcggtccacg cccacaatcc gcttgtcact ccacctgacg
    18361 aacgtgacga agctagagac gttgtcttga acgtcccact gtgtagcgtt cgggtggtca
    18421 tctgcgaaag ccggatcctc ggtattcact tgtgcctggg ctcgagggtc gggcacaaga
    18481 cccaaacgct cgtactcaca ctcaagttca tcttgggtca ggtctcggcc gagattgaaa
    18541 aagtggactt ccacctcttc gccctcgcct tgaggcatgg tggcgagtac gtcagggttg
    18601 acatactgcg ttcgtcctgt ggcctcaatc acctgctctg gtgtgagggt gcggttcact
    18661 ttcacacggc ggatgatggt agtcgcgttc tggccttctt tatagtcctg atgtccagct
    18721 gccattctgt ttctttcctt tcttcagttg gaaaagagca atgatactta atcataaatt
    18781 tattaatgtg tcaatattgt agatacttaa tactttgtac ttaatactct atacttattc
    18841 atatgttgat ctacgacaca ttatccggtg aaaagaaaaa gttagaacaa acaaaagata
    18901 gaataaacct ttttgtctgc ggaccgactg tttatgacta ttcgcatata ggacacgcaa
    18961 gaacctatat tttctttgac ctctttgtta aatatctgcg ttctctcgga tataaaatat
    19021 tttatctgca aaatattacg gatattgatg acaggattat cgtcagggct aaagaacgaa
    19081 gcactgaagt gcaaaaatta gctaaggaat ttacggataa gtatttagaa gatatgaagg
    19141 cgcttggggt gactgaagtt gataagtatg cgccagcgac gaaatttata aaacagattg
    19201 tcggacaggt taaaacttta atagacaaag gttacgcgta taaaacaagc gatgggtatt
    19261 atttcgacat ttctaagttc gaagattatg gaaagctctc taggcggagc gccgaacagg
    19321 cggagagcgc cgtatcgcgt atagatgaaa gcgttacaaa aaataataaa ggcgactttg
    19381 ccctttggaa attttataaa gaaggggaac cgttttggga aacggaatta ggcaagggta
    19441 ggccgggctg gcacatagaa gataccgcaa ttaccgagaa atattttggc ccgcagtatg
    19501 atattcatgg cggagggata gatttgaaat tcccgcatca cgaagccgag cttgctcaac
    19561 aggaatctgc ttcgggcaag agcccgtttg taaaaatctg gatgcatagc ggatttttgc
    19621 ttgcagacgg gaaaaaaatg tcaaaaagtc tgaataattt tattacgatt ggcgattttt
    19681 tgactaaaca tgatcctttg gttttaaagt ggattgtcct cactcaccac tatcgctctc
    19741 cggttaacta tactgagagt cttatatctc aatctatttc tgccataaat ggattaagag
    19801 agtcgttggc taaattagga tttgcttcta ataagtcgaa taaagattct tcgtttgatt
    19861 tgcaagaaaa gttgaagaat gtggttatag agtttgaaag cgcccttagt gatgatttta
    19921 atactccgct ggccattgct gtaattctat cttttttggg cgacataaat gataaaatct
    19981 ggaatttaag caaaatcgag gctaaatcaa tattaaaaat aatcaaaaac acgctttcag
    20041 tcttttctat cgagttaaag gctgacgcaa tccctctgaa tattaaaaaa tttatagatg
    20101 aaagggagga attcagaaga aataaacagt ttatccagtc tgacgccttg agaaaaaaaa
    20161 cggaagactt aggatatata atagaggata cgccgttcgg cccctttatt ataaaaacta
    20221 atgcaatcaa ttaaagattt aaaagttcaa cctcaagatt tagacgccga gcagtccgta
    20281 ctgggcgcgc taatgattga taaaaacgct attttccaga tagcagatgt tttaaacccc
    20341 ggagacttct ataaaagatc acattctatt atttttgaag ccatactcca gctttggcaa
    20401 agacacgagc ctatagacat tttaagcgtg actgcagagc ttaaaaaaat ggataagtta
    20461 aaagacgctg gcggatcaag ttatctgacg gagataataa attctgttcc tacggcctct
    20521 catgttgctc attatgccaa gatcgtaaaa gagaaaagaa ttttaagaga gcttatcaac
    20581 gcctccgctg agattaccga agatgcgttt aatcctgatg atgatttgga aactcttctt
    20641 gataaaatag aacagagagt tttttctata tcacaaaaat caattgccaa aaacttcgtc
    20701 cctctaaaag aggagttgaa gggagcatat gagagaatag aaaaattgca tcagggagga
    20761 ggcgcgggaa taagaggagt gccaagcgga tttatcggac tggacaatct attatcggga
    20821 tttcaaaaat cggatttaat tatcgttgga gctcggccat cgtatggaaa aacagctttc
    20881 gttctggata ttgcaagaca ggcggcttta agtggaacaa cagttgcgtt tttctctctt
    20941 gaaatgagcc gcgagcaggt tgtagatagg ttgatttcat ctgaagctca agtggactta
    21001 tggaaattaa ggacaggcag acttaaagat gaatcggaat tcgcgatgat tcaaaacgca
    21061 ttaggcaagc ttaataacgc tccatttttt atagatgaca ctccctcgcc aaacgttgtt
    21121 caaatgcgtt ctatggcaag gcgtttacaa gctga
//
LOCUS       DATDNZ010000048         6467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3512095, whole genome shotgun sequence.
ACCESSION   DATDNZ010000048 DATDNZ010000000
VERSION     DATDNZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512095"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1116
                     /locus_tag="VI432_01905"
     CDS             193..1116
                     /locus_tag="VI432_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496881.1"
                     /translation="MLKRKKNNTAILIIISLLFVFGISNVSAQTSSTGSSGGFFQALS
                     FNNIINQLKSFGELFLEGFNFKPVLPTTVPTTGLSPTSVPTSGPTPASSQWCDFRNDL
                     SSCTGGDQFVNSDGLTGQECFEFCEGIYAPVQATGCWILAGNGNCYCREGIVNLGGSR
                     PGGQCPAPYTQSSYYGQSNYYIQSLYYGQSSYYGQSSYYNQGVYYTQSQYYSQSLYTT
                     SECPPGYIGHPSVGCIPWPPYTQSIYDTGSCPNDYIGAPNYGIGCQYIQAGYNIYTQA
                     TYIYSQSSYYTQSSYYSQNQYYAQSSYQSGY"
     gene            1227..1451
                     /locus_tag="VI432_01910"
     CDS             1227..1451
                     /locus_tag="VI432_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496882.1"
                     /translation="MSKQNLILTLVVLGSILLITISSLTTRISTPSASPENIVEALTV
                     NNIKNQIEALQTLLAEGIAFRSYYEPLQEN"
     gene            1494..2285
                     /locus_tag="VI432_01915"
     CDS             1494..2285
                     /locus_tag="VI432_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496883.1"
                     /translation="MKRFLKQFLYGLLYLSILTLIGFGVYVLFFIEKPTCFDNIQNQL
                     ETDVDCGPEVGISATGPFTPGGCLPCEIKTITLFTDEPRIFPVGPESSSIIVGVENKS
                     FNYGLKEFDYSIEVISKLGTRLALFERSSYIAPQEKKYLIISNLNLDARDIASANLIF
                     DKNKLNWLPPSSLSEKPNLEISDVSISGDGDEFKKIEGILTNNSSNNFNRIILAAFIY
                     GNDNDILSVSTTELRTIKAFENYAFTIFLPDVEYKDIEIISDIIP"
     gene            2331..3410
                     /locus_tag="VI432_01920"
     CDS             2331..3410
                     /locus_tag="VI432_01920"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY4496884.1"
                     /translation="MKAVAKYDWWLVGSVLMLILISVVTLSSIAPKSDLYKQVVWIFI
                     GLGAIFAVPMINLRPLFAYRWFVLSIYFGILGLLALTYFFAPTISGVRSWLVFGDVQI
                     QSSEFMKLALIILFSKFFAKKHVAIARLGIIIASFIYFLIPAFMILAQPDLGTIIIIF
                     ALWLGYVLVSGLPTRYIAIMLLIAVIFGFIAWNLFLYDYQKERIIGLFNPEYDPLGIN
                     YSAIQAKIAIGSGGFWGKGFGQGTQTHLGFLPAATTDFVFAAFIEEWGVVGGFVLIAA
                     FVLMIYRILRVGILSNNNFSRFIAVGAVIFFLSHLFINVGSNVGLVPVIGIGLPFVSY
                     GGSNFLTASIMVGIILNIQRHKHGF"
     gene            3414..4124
                     /gene="mltG"
                     /locus_tag="VI432_01925"
     CDS             3414..4124
                     /gene="mltG"
                     /locus_tag="VI432_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4496885.1"
                     /translation="MRRLIILTGGALIALIVFLILLNGFYLRLNRPVVKSVTIPEGLS
                     NREITALLVESDILIDEELPEELEGYLFPDTYEFFVPSSLDFVVDKMSRRFNEKILSI
                     IPEDKDVREIIIAASLIEEEVSSPEDRMIVSDIIWKRIKRGMFLEIDASICYIKPAPC
                     HPIFKEDFKIDSKYNTYLYKGLPPGPITNPGLGSVEAAINPKQSPYWFYLSSSEGGET
                     IFASYLDEHRANIVKYLK"
     gene            4248..>6467
                     /locus_tag="VI432_01930"
     CDS             4248..>6467
                     /locus_tag="VI432_01930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY4496886.1"
                     /translation="MSTHQTKLPVKNFSSQRGSLINPPDLVAIQTQSYDWFRKDGLKE
                     LFEEISPIKDYSGKDLELYFTDYYFDEPKLNEKQSGMKSVTYEAPLRAKVKLTDKKGD
                     LMREQEVYLGDFPVMTERGTFLINGVERVVISQLIRSPGVYFSSSFYRGKKLFGAKIV
                     PNRGTWLEFESDINGFIGVKIDRHRKVAATSLLRIFGLTNDEMTKIFGTNLEETFKKD
                     SAKNSEDSYLEIYKRIRPGDLATADDAKKLIDAMFNQADRYDLSEVGRFKLNQRLQTK
                     SKSRLLMKEDIIAIVKEVIRLNGDSKAEEDDIDHLGNRRVRAVGELLQNRLRIGFSRM
                     RRNVQDRMSTAEVETVTPVQLINSRPLVSIVQEFFSSSQMSQFMGQTNPLDELEHKRR
                     VTSLGPGGLTRERAGVEVRDVHGSYYGRICPIQTPEGQNIGLVNQFAGFSKLNEHGFL
                     ITPYVKVQNRKITKEILWLDAIEEEKYKIAVGNVSFDEKDRIIEDQVDSKIKGEAGRC
                     SADEVELINVAPDQFLSIATSLIPFLEHDDANRALMASNMQRQAVPCIKPSAPYVGTG
                     VEDVVARNSGHLVIAELDGVVTEVDAAHISIKGKDNKVKKYELNKFKMSNQYMSISQK
                     PVVMKGDKVKDGDVLADGQSMDKGTLALGQNLVVAFMPFEGANYEDAIVLSEKVQRDD
                     LFTSVHIEEFEVDVRDTKLGPEITTPDIPNVSEDKLKNLDEDGIIRIGAEVRGGDILV
                     "
BASE COUNT         1954 a         1084 c         1391 g         2038 t
ORIGIN      
        1 aataggatgt caatatatac aggctggtta caatatttat actcaagccc aatatgtcta
       61 cacccaatct tcttactaca atcaatctag ctattactct caaagccaat attacgttca
      121 atcgcagtat caggcagggt attagattag gtgatctaaa gaaatagtta taatatttgg
      181 ttgagcttca aaatgttaaa gagaaaaaag aacaatacag cgatactcat tataatctca
      241 ttattattcg tttttgggat tagcaatgta tcagctcaaa cttcttctac cggttcttct
      301 ggtggatttt tccaggcgtt aagctttaat aatataatca atcaacttaa atctttcgga
      361 gaattattct tagaaggatt taatttcaaa cctgtgttgc ccacaacggt cccgacgacc
      421 ggcctatcgc cgacatcagt gccaacatcg gggccaacgc cagcttcaag tcaatggtgc
      481 gattttagga atgatctttc ttcgtgtaca ggaggagatc agtttgtaaa tagtgatgga
      541 ttaactggtc aggaatgttt tgaattttgt gaaggaattt atgcgccagt tcaagctact
      601 ggatgctgga tattagcagg taatgggaat tgttattgca gagaaggaat agtcaattta
      661 ggcggatcaa gaccaggagg acaatgtccc gcgccatata cgcagtcttc ctattacggc
      721 caatcaaatt attatattca atctttgtat tatggtcagt cgagttatta cggccaatca
      781 tcctactata atcagggcgt ttattacacg caatctcaat attatagtca gtcattatat
      841 acaacatctg aatgtccgcc gggctatata ggtcatcctt cagtcggatg tattccatgg
      901 ccgccatata ctcaatctat ttatgatacc ggctcatgcc cgaatgatta tatcggtgcg
      961 cccaactacg gaataggatg ccaatatata caggctggtt ataatattta tacccaagcc
     1021 acctatattt attcccaatc ctcttactac acccaatcaa gttattactc tcaaaaccaa
     1081 tattatgctc agtcttccta tcagtctggt tattgatttt tgttgttggc atttcaaaat
     1141 ataagagagt tagattaaaa ggctggaaga gcaatccgta cgaccagagg atttaactag
     1201 taattttttt atgataatat tctgtcatgt ctaaacaaaa cttgattttg acgcttgttg
     1261 ttcttggatc aattttatta attacaattt ctagtttgac gactagaatc tctacgccat
     1321 ctgcctctcc ggagaatata gtagaggctt tgaccgttaa caatatcaaa aatcaaattg
     1381 aggctctaca aacattactc gctgaaggaa tagccttcag gtcttattac gaacccttac
     1441 aagaaaatta ataggcgctt taaatgcgtc ggcaataaag ttagaatacc tttatgaaaa
     1501 ggttcttaaa gcagttttta tatggcttgc tttatttgag tatattgact cttatcggat
     1561 ttggggtgta tgtcttattt tttattgaaa agccgacctg tttcgataat atacaaaatc
     1621 aattagagac agatgtggat tgcggtcctg aagtcggtat atcggcaacg ggtccgttta
     1681 cgcccggagg atgcttgcct tgcgaaatta aaacaatcac tctttttaca gatgagccga
     1741 ggattttccc cgtcggtcct gagagctctt cgattattgt tggcgttgaa aataaaagtt
     1801 ttaactacgg cctcaaagag tttgattatt caatagaggt gatcagtaag ttagggacaa
     1861 gactcgctct tttcgagagg agttcttata tagctccaca ggaaaagaag tatcttataa
     1921 tttctaatct taatttggat gccagggaca tagcatcggc taatctaatc ttcgataaaa
     1981 ataaattaaa ctggttgcca ccatcgtctc tatctgaaaa gccaaatctt gaaatttcgg
     2041 acgtctcgat ctcaggagat ggagacgagt ttaaaaagat agaaggaatt ttaacgaata
     2101 attcttcaaa taattttaat aggattattc ttgcggcatt tatttatgga aacgataacg
     2161 atattcttag cgtttccacc acagaattaa gaaccattaa agcttttgaa aattacgcat
     2221 ttacaatttt cctgccagat gtcgaataca aagatataga aataatatct gacatcatcc
     2281 cataatcggg tatacaattt ttagttttta aacatagata aagtttataa atgaaagctg
     2341 ttgcaaaata cgattggtgg ctggttgggt cggttttaat gcttatttta attagcgttg
     2401 taacgcttag ctcaatcgcg cctaagagcg atttatataa gcaggtggtt tggattttta
     2461 tcggattggg agctatattc gcagttccaa tgataaattt acggccactt tttgcttatc
     2521 gctggtttgt cctttctata tattttggga ttttagggct gttagcgctc acatattttt
     2581 ttgcgcctac aatatccgga gtaagaagct ggcttgtttt tggggacgtg cagattcagt
     2641 cgtcggagtt catgaagctt gctctcataa ttctattctc taaatttttt gctaagaaac
     2701 acgtcgcgat agcgaggctg gggataatca tagcctcatt catatatttt ttgataccgg
     2761 cttttatgat cctggctcag ccggatttag gaacgatcat aataattttt gctctttggc
     2821 tgggttatgt tctcgtgagc ggattaccca ccagatatat cgccataatg ttattaatcg
     2881 cagtaatatt cggttttata gcttggaatc tatttttata tgattatcaa aaagagcgta
     2941 ttatcggcct attcaatcct gaatatgacc ctctgggcat caattacagc gccattcagg
     3001 ctaagattgc tatcggttct ggcggttttt ggggtaaggg ttttggtcag ggtacgcaga
     3061 ctcatttagg gtttttgcca gcggcaacga cagattttgt tttcgccgcc tttatagagg
     3121 agtggggagt ggttgggggc ttcgttttaa ttgctgcatt tgtattaatg atttatagaa
     3181 ttttaagagt cggcatatta agcaataata atttttctag gtttatagcg gttggagcgg
     3241 ttatattttt tttaagtcat ctgtttatca acgtcggatc taatgttgga ttggtgcctg
     3301 ttatcgggat tggcttgccc tttgtgagtt atggcggatc taactttttg acagcaagta
     3361 ttatggtcgg tattatttta aacattcaac ggcacaaaca tggattttga taaatgagaa
     3421 gattaattat tttaaccggg ggcgcgctaa tagccttgat agtatttcta atattattga
     3481 acggttttta tcttcgtctt aataggcctg ttgtaaagtc tgttaccatt cctgagggat
     3541 taagcaatag agaaataaca gcgctcctcg tagagtctga tattttaata gacgaagaac
     3601 tgccagaaga attagaaggt tatcttttcc cggacacata tgaatttttt gttccttctt
     3661 ctctggattt tgtggttgat aaaatgagta ggagatttaa tgaaaaaatt ttatcgatta
     3721 ttccggaaga taaagacgtc cgcgaaataa taattgccgc atcccttata gaagaagaag
     3781 tgtcttcccc tgaagacagg atgattgtgt ctgatattat ttggaagagg attaaacgcg
     3841 gcatgttttt agaaattgat gcgtcaattt gttatataaa gccagcgcca tgccacccta
     3901 tttttaagga agattttaag attgattcaa agtataatac ttatttatat aagggtttgc
     3961 ctcctggtcc gataaccaat ccggggctag gatcggttga agcggcgatt aatccgaagc
     4021 agtcgccata ttggttttat ttgtcttctt cggagggggg agagactatt ttcgctagct
     4081 atcttgacga acatagggca aatatagtta aataccttaa gtaaattatc gaaatatcaa
     4141 accgctatca ataagtgggt ttttattttt tactccttgt gactatggat gggttatcgg
     4201 aagcagggat tgtttataaa aagtcgttat tattaaaaaa aaacaatatg agcacccatc
     4261 aaacaaaact tccggttaag aatttttcat cccagcgcgg ttctttaatc aacccgccgg
     4321 acctagtcgc gattcagacc caatcctatg attggtttag aaaagatgga ctgaaagaat
     4381 tatttgaaga gatatctcca attaaagatt attccggtaa agacttggaa ttatatttta
     4441 ccgattatta ttttgacgaa ccgaagctaa atgagaagca gtcgggcatg aaaagcgtta
     4501 cttatgaagc tcctcttcga gccaaggtaa agcttacaga taaaaaagga gacctcatga
     4561 gagaacaaga ggtttattta ggtgattttc ctgtcatgac agagagggga acgtttctta
     4621 taaatggagt tgagagagtc gttatttccc agcttatcag atccccaggc gtatatttta
     4681 gctccagttt ctataggggc aagaaattat tcggcgccaa gatcgttcct aatcgtggaa
     4741 catggcttga gtttgaatca gacatcaatg gatttattgg cgttaaaata gataggcacc
     4801 gtaaggtggc ggctacatca ttattgagaa tttttggttt gaccaatgac gaaatgacta
     4861 agatctttgg aacaaatctt gaggaaacgt ttaaaaagga ttcagcaaaa aattcagagg
     4921 attcgtattt agagatatat aaaagaataa gaccaggaga tctggcaacg gcagatgacg
     4981 ctaaaaagtt aattgatgcc atgttcaatc aggctgatag atatgacttg tctgaagtcg
     5041 gaaggtttaa gttgaaccag aggcttcaaa ccaagtctaa gagcagattg cttatgaaag
     5101 aagatataat cgctatagta aaagaagtta ttcgtttgaa tggcgattca aaggctgagg
     5161 aagacgatat agatcatttg gggaataggc gagtaagagc ggttggagag cttttgcaaa
     5221 ataggctgag aatcggtttt tcccgcatga gaagaaatgt tcaggataga atgtctacag
     5281 ccgaagttga aactgttacg ccagttcaac ttattaattc gagaccgctt gtttctatag
     5341 ttcaagaatt tttttcgtct tcacagatgt ctcagtttat gggacaaacc aacccattag
     5401 acgaattaga gcataagagg cgtgttacat cccttggccc aggaggactc actcgtgaac
     5461 gagccggagt tgaggtgcgt gatgtgcacg gatcgtatta tggcagaata tgtccgattc
     5521 aaactcccga gggtcaaaat atagggctgg ttaaccagtt tgccggtttt agcaagctta
     5581 acgagcatgg gtttttaatt actccttacg ttaaggttca aaatagaaaa ataaccaaag
     5641 agatattgtg gcttgatgct attgaagagg aaaaatataa aattgcggtt ggaaatgttt
     5701 cttttgatga aaaggataga attattgaag atcaggttga tagtaaaatt aaaggagaag
     5761 ctggaaggtg tagcgctgat gaggttgagt tgattaatgt agctccagat caattcttaa
     5821 gcatcgcaac atctcttatt ccatttttgg agcacgacga tgctaaccgc gcccttatgg
     5881 cttcaaacat gcagagacag gccgttccat gtattaaacc cagcgctccg tatgtcggaa
     5941 ctggagtaga ggatgtggtt gcgaggaatt cgggacatct tgtgatcgca gaattagatg
     6001 gcgtggttac tgaagttgat gcggcacaca tttctataaa aggaaaagac aataaggtta
     6061 aaaagtatga acttaacaag ttcaaaatgt ctaaccaata tatgtctatt tctcaaaagc
     6121 ctgtcgttat gaagggcgac aaagttaaag atggagacgt gttggctgat ggacaatcta
     6181 tggataaagg aacattggca ttgggacaaa acctggttgt tgcttttatg ccgtttgagg
     6241 gagcaaatta tgaagatgct atagtgcttt cagaaaaagt tcagagagat gatttattta
     6301 catctgtgca tattgaagaa tttgaagttg atgtgagaga taccaaactt ggtcctgaaa
     6361 tcaccactcc ggacattccg aatgtttcgg aagataaact taaaaatctt gatgaagacg
     6421 ggataataag aattggcgcc gaggttcgcg gaggagatat tttagtc
//
LOCUS       DATDNZ010000049         4550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1442410, whole genome shotgun sequence.
ACCESSION   DATDNZ010000049 DATDNZ010000000
VERSION     DATDNZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442410"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VI432_01935"
     CDS             complement(<1..952)
                     /locus_tag="VI432_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY4496887.1"
                     /translation="MNMNKEDLLQELAIKVNLGEISREEVTARLQSVRPTSGNGVDSE
                     GSHFNVTKMLYVLGGAIVIVGIGIFIARIWTDIGSLGRIAVTLGLGLTLALIGSLLFK
                     KEKGGDIGSVFHFLGGFLISGGATVALSEFNIKNDWAFVATSATTFLFYMLLSGFHKR
                     AILTFFAIANATSTIYLILNAIIGGPFRGTFQIEDIYKYTTILIGISYILLAYAFKTG
                     WSSKLIGALYFFGSIFFLSPAFTNIFDSMFWQFIYLIFLSVGFSASVYIRSRIVLIFS
                     TIFLLAYISYITSEYFADSIGWPISLVILGLSFIGLGYASI"
     gene            complement(949..1536)
                     /locus_tag="VI432_01940"
     CDS             complement(949..1536)
                     /locus_tag="VI432_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496888.1"
                     /translation="MKQFIKKNIVIILAFSLPVLFILIIFLTTFLPSVFISTDYNFIY
                     ATCAEGTDYYCGNYLQKLYSIQDERLVVNQVDVDKLVKDLYGEYPPRSGVAYSSRIFL
                     HDTKSNESKEIEIKEAETLNLNSLLTSPDGVTVSSDYDSGGGFFPFFESGSSYGYYLT
                     KGKNKSRLNLVNDDERYYYKDSFRFIGWVIPGRNK"
     gene            complement(1634..2482)
                     /locus_tag="VI432_01945"
     CDS             complement(1634..2482)
                     /locus_tag="VI432_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY4496889.1"
                     /translation="MFPHKESERLFLIISILLFWIVVHATLHEGGHAFFMRRYGVEID
                     KMGIGLPINPKLQFRLRGVSFPLVVSPWLISAYVKMTNAGFVSMDSLPYGKRADINSA
                     GIMANLISAFVLEMIFLAVVGGAVWKIAIAGIILTVFLIIFYKVFCRILIPFLGSALG
                     IVVAYDLLQMMAKFFSQGSMASDDIGVGGPISIVVLIQSMKASIPGGMTGFFLQTGAI
                     LGFVFATTNMLPLRPLDGGNIFVDLVEKFFGGRVARVADWMGVLALLMIVALAFTADI
                     LKVFGR"
     gene            complement(2510..2596)
                     /locus_tag="VI432_01950"
     tRNA            complement(2510..2596)
                     /locus_tag="VI432_01950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2561..2563),aa:Leu,seq:taa)
     gene            complement(2629..2700)
                     /locus_tag="VI432_01955"
     tRNA            complement(2629..2700)
                     /locus_tag="VI432_01955"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2666..2668),aa:Cys,seq:gca)
     gene            complement(2718..3071)
                     /gene="rbfA"
                     /locus_tag="VI432_01960"
     CDS             complement(2718..3071)
                     /gene="rbfA"
                     /locus_tag="VI432_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY4496890.1"
                     /translation="MANRNLRLNSLLRSELNKLILKELDLPDGVLATISDVAVAKNLK
                     NADVLISVFPSDRGEEIIKTFSRWQGEAQFLLNRKLNIRPVPRIRFVLDEGLGNAAKI
                     EKLMMDTVDKTEENN"
     gene            complement(3075..>4550)
                     /locus_tag="VI432_01965"
     CDS             complement(3075..>4550)
                     /locus_tag="VI432_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY4496891.1"
                     /translation="NTNKNQASRSPIIVVMGHVDHGKTSLLDYIRKTSLVEKEAGGIT
                     QSIGAYEVEVRSGGEPKSREIEANRGAPRQAREDFAGRKITFIDTPGHEAFSKMRSRG
                     AHTADLAILVVASDEGVKPQTKEAIKILKDTETPYIVAITKIDRKNANIEKVKNELLS
                     EGVLLEGLGGDVSWQGVSVETGEGIEDLLGLILLLAEIENLTYDSKAPGRGVIIESHK
                     DSRKGIIANCVLRDGILKIGDPIATASSKGKIKILEDFMGKKLKEVRPSSPVSIMGFE
                     SIPSVGEEFWIGEGSAPVKSGQDLFAKAAEGEMRLRAILKASNSGSLEVLKSVLENQV
                     FTVNSSVGDVTSNDIQEAINTGSVIVNFNSKMDKSALNLADVHKIKIFKSAIIYKLVE
                     EIEAYLKGEAETEPIGSLEILKVFEEKGRRRVIGGRVVGGVIKNNADIQIERNGNIVG
                     EGRVINIQSQKKDVAQIEEGECGMLIETEARISSGDTLKAF"
BASE COUNT         1398 a         1096 c          744 g         1312 t
ORIGIN      
        1 taattgaagc gtaaccaaga cctataaatg ataaacctaa aataaccaat gaaatcggcc
       61 aaccgataga atccgcgaaa tattcacttg ttatataaga aatataagct agcaaaaata
      121 tcgtgctaaa aataagcaca atccgactcc ttatataaac ggaggcagag aatccaactg
      181 aaagaaaaat aagatagata aattgccaga acattgaatc aaaaatattg gtaaacgccg
      241 gggataaaaa gaaaatgctg ccaaaaaaat aaagagcgcc tataagctta ctgctccagc
      301 ctgtcttaaa agcataagca agaagaatat aacttattcc tattaaaata gtcgtgtatt
      361 tataaatatc ttctatttga aatgttcccc tgaacggacc gcctattata gcgtttagaa
      421 tcaaatatat tgtcgaggtg gcattcgcaa tcgcaaaaaa tgtaagaata gcgcgtttat
      481 ggaaacccga cagaagcata tagaaaagaa aggtggtggc cgatgtcgca acaaatgccc
      541 aatcattctt tatattaaat tcagaaaggg caacggttgc tccgccagaa attaaaaatc
      601 cacccaagaa atgaaaaaca gaaccgatat ctccgccctt ttctttttta aacaaaagcg
      661 aacctataag agccagcgtc aaaccaagcc caagagtaac agcaatccga cccaaagatc
      721 cgatatcagt ccagatgcgc gctataaaaa tacctattcc aacaataact attgctccac
      781 ctaaaacata gagcattttt gtaacgttaa aatgcgaccc ctcgctatct acgccatttc
      841 ctgaagttgg gcgaaccgac tgcaacctcg ccgtaacctc ctctctgctt atctcgccta
      901 aattaacttt tatggcgagc tcttgtaata aatcttcttt gttcatattc atttatttct
      961 tcccggaata acccaaccga taaatctaaa gctgtcctta taataatatc tctcatcgtc
     1021 atttacgaga ttcaacctac ttttattttt acccttagta agataataac cataagacga
     1081 tccgctttca aaaaagggaa agaagccgcc gccactatca taatcacttg aaacggtcac
     1141 tccgtctggt gaagttaaaa gactgttaag attaagtgtt tccgcctctt taatttctat
     1201 ttccttgctt tcattgctct tcgtatcatg caaaaaaata cgagaagaat atgcaacccc
     1261 tgaccttggc ggatattcgc catataaatc ttttactaat ttatcaacgt caacttgatt
     1321 tacaaccaat ctttcgtcct gaatggaata taacttctgt aggtaattgc cgcagtaata
     1381 atccgtccct tcagcgcaag ttgcatatat aaaattatag tcagttgaaa taaaaactga
     1441 aggcaggaat gtcgtcaaaa agattattaa aatgaataac accggcaagg aaaacgccaa
     1501 tatgataaca atattctttt ttataaattg tttcatttgt tgattgaaat tatagacgac
     1561 taataggctt tttaaaagat ccaacactca gacaataaaa aagactggcg gataagatca
     1621 gccagtcata attttacctc ccgaatacct tcaaaatatc tgccgtgaaa gcgagagcaa
     1681 ctatcatcaa tagggcgagc acgcccatcc agtcggctac acgagccact ctcccgccga
     1741 aaaatttttc aacaagatca acgaagatat ttccaccatc caggggtctg agcggaagca
     1801 tgttcgtggt ggcgaataca aaacctagaa tggctcctgt ctgcaagaaa aatcctgtca
     1861 ttccgcccgg aatggaagct ttcatgctct gaatcaaaac gacaatgctt atcggaccgc
     1921 caacaccgat atcatcggat gccatactcc cctggctaaa gaatttagcc atcatttgaa
     1981 gaagatcata ggccacaact attccaagcg cagaacctag aaatggaata agtatcctgc
     2041 aaaacacttt ataaaaaatg atcaaaaata cggttaagat gatcccagcg atagctattt
     2101 tccatactgc gccgccaaca acagccaaaa aaatcatttc caagacgaag gcagagataa
     2161 gattagccat gatgccagcg ctattaatat cggccctctt cccataagga agagagtcca
     2221 tgcttacgaa gcctgcattt gtcattttca cataagccga gattaaccac ggggaaacga
     2281 ctaatgggaa cgaaacccct ctcaaccgga actgtagctt agggttaatc ggaagaccga
     2341 ttcccatctt atctatttca acgccgtacc gcctcataaa aaaggcgtgg cccccttcgt
     2401 gcaaggtagc gtgcacaacg atccagaaca gaagaattga gattataagg aacagtcttt
     2461 cactctcctt atgcgggaaa ataacacttt aatagtggat agtcaattct ggtgcccggg
     2521 gtgggattcg aacccacaac ccaaaaattg ggcaaggatt ttaagtcctc tgcgtaaacc
     2581 attccgccac ccgggcataa atcaccttat ctttaaaaag aaaggttgag gccgggatgg
     2641 gaatcgaacc cacgcataag ggatttgcag tccctcgtct taccacttga ctacccggcc
     2701 agatatcttt atccagtcta attattctcc tctgttttat caactgtatc catcatcaac
     2761 ttctcaatct tagcagcatt cccgagaccc tcgtctaaaa caaatctaat tcttggtaca
     2821 ggcctaatat ttaattttct attgagtaga aattgggcct cgccttgcca cctgctaaat
     2881 gtcttgataa tctcttctcc tctatcggac gggaaaacgc taatcagcac gtctgcattt
     2941 tttaaatttt ttgcgacagc cacatcgcta atagtcgcga gcactccatc cggcaaatcc
     3001 aactctttta aaatcaattt gtttaactcc gatcgaagaa gagagtttaa tcttaaattt
     3061 ctgtttgcca tatattagaa agctttcagc gtatctccag aagatattct tgcctctgtt
     3121 tcgataagca ttccgcactc gccctcttct atctgcgcca cgtccttctt ttgagactga
     3181 atattaatca ccctaccctc accaactata ttgccattcc tttctatttg aatatctgca
     3241 ttatttttaa taactcctcc cacaactctg ccgcctataa ctcttcgcct tcctttctct
     3301 tcaaatactt ttaaaatctc tagagagcct atcggttctg tctccgcttc accttttaaa
     3361 taagcctcta tctcttcaac caatttataa ataatggctg atttaaatat tttaatttta
     3421 tgaacatccg ccaaatttaa tgcggattta tccattttag aattaaaatt gactattacg
     3481 cttccggtat ttatcgcttc ttgaatgtca tttgatgtca cgtctccaac agaactattt
     3541 actgtgaaaa cctgattctc taaaacactt tttaagactt ccaaggatcc ggaattgctg
     3601 gcttttaata tcgcccttaa tctcatttcc ccttctgcgg cttttgcgaa aagatcttgt
     3661 ccgcttttta ccggcgcgct accttctcca atccagaatt cctcccccac tgacggtatt
     3721 gattcaaacc ccataattga aactggagat gatgggcgca cctccttcaa ttttttaccc
     3781 ataaagtctt ccaagatttt aatttttcct tttgaagagg cggttgcgat cggatctcct
     3841 atttttagaa ttccgtctct taaaacgcaa tttgctatta tccctttccg gctatcctta
     3901 tgagactcta taatcacccc tctgcctgga gccttagagt cataggtcaa attttctatt
     3961 tcagccagca gcaatatcaa acccaataaa tcttctatgc cttctccagt ttcaacagag
     4021 actccttgcc aactgacatc tccgcccaaa ccttcaagca gaacgccttc tgacaacaat
     4081 tcatttttaa ctttctcgat atttgcattt ttcctatcaa ttttagtaat cgcaactata
     4141 tatggagttt ccgtgtcttt taaaatttta atggcttctt tcgtctgcgg tttcacgcct
     4201 tcgtcagaag caacgaccaa tatcgctaga tccgctgtat gcgctcctct gcttctcatt
     4261 ttagagaaag cttcatggcc cggcgtatca ataaaagtga ttttgcgacc agcaaaatct
     4321 tcccgagcct gccgaggagc ccctcgattc gcttcgattt ctcgagattt cggttcgccc
     4381 ccacttcgaa cctcaacttc gtatgcgcca attgactgcg taattccccc cgcctccttt
     4441 tcaactaaac tggtttttct gatgtagtcc aaaagcgaag ttttaccgtg atcaacgtgc
     4501 cccattacaa ctataatcgg tgagcgggaa gcctgatttt tattagtgtt
//
LOCUS       DATDNZ010000050        12182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1194928, whole genome shotgun sequence.
ACCESSION   DATDNZ010000050 DATDNZ010000000
VERSION     DATDNZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194928"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VI432_01970"
     CDS             complement(<1..507)
                     /locus_tag="VI432_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY4496892.1"
                     /translation="MTTTETHTLGRKLQSLSPSLRAKIIIGERHFKPNPNTPISNWLV
                     DTKRHCNGGKTVDAGLWLKTHCDQGGKIFLTMAGAGSSFQMGIAISKLIQAGKIAAIS
                     VTGANMEESLYRLVANSHYAYIPNYQELTPEEEKELDRAGFRRITDTFLPEDESVRVV
                     LDPLMDLWR"
     gene            complement(659..1093)
                     /locus_tag="VI432_01975"
     CDS             complement(659..1093)
                     /locus_tag="VI432_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496893.1"
                     /translation="MQSLKKNRITGGSLANNLPLMKALLARNDIKVHSTGKLVFSEEN
                     RGSLERELKYLFDSQRIVLLTSRVFGGMFVCTEFEDLLLLSDRSSRVQVINEAWSVPK
                     NLWELIKPSLLEFGAKFIEPRGKIKSANIAPKTSTEEQGTLH"
     gene            complement(1149..2309)
                     /locus_tag="VI432_01980"
     CDS             complement(1149..2309)
                     /locus_tag="VI432_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496894.1"
                     /translation="MFGSKRNLLIISILIGVAGYLIYSTPPGSPKEAFAVLYNKCADQ
                     SISDREKGECLKNLAETASRAYQLQEIEDGIRNINDSYKLQWCHEFMHYLGWETLSKT
                     KTISKAFELASGLCDSGMYHGIVEEYINQAELSYDPETFTKNIIYETCEESINNSKNP
                     VAIKGLCYHGLGHAFMFITENKLNNALEYCDRTKSPNGCYTGAFMENIQTKQIARFSH
                     QKELAFQENDPDYPCKILNNKYKDTCYLYKGINNLVATGGDVKKAFELCLKVASKYKN
                     ICFRGTGSNIPGPHETPKGAGEKCLLSLQINKTAYEECIKGAMSFLVQVALGSPEAVA
                     EFCETIKEEQRNLCYAESGDNLNLWVTENETLADKCNKLPTSDAKNLCLNGR"
     gene            complement(2343..3182)
                     /locus_tag="VI432_01985"
     CDS             complement(2343..3182)
                     /locus_tag="VI432_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016037283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY4496895.1"
                     /translation="MFNNYIATKNIIEFDEAINKLRAFFKDRGFTEMHTQDRLSILAA
                     CEDPSTIATFNYAGEIWPLPQTGQMWLEYELLKNPKLEGVFCVSTSFRDEPNPIPQRH
                     NIIFPMFEFESRGTMNDLRKLEKDLCKAMGFNEKSIHHTYEELADHYGTVELTHDHEM
                     KIKDDFSAVTFIEMFPQKTSPFWNMRKNGDYAHKIDVILHGMETIGSAERSINKNEMN
                     EQFHTISDGKYAETLHNIFGKERVEKELKDFLSFDFFPRFGGGIGITRLIRAMNMERA
                     AQQ"
     gene            3536..3934
                     /locus_tag="VI432_01990"
     CDS             3536..3934
                     /locus_tag="VI432_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496896.1"
                     /translation="MTGTLKKEIHMDDVILFFMCRLAEGGQEERIVLPTSKIHRSLAD
                     LGPMLERLEVFARFHRDPVETHSQQVNEAVAHLVPFALESSWKYGEGTRLELAKTAVP
                     PRMENLKQNLSPSVIDLLEANLQKFQAGLG"
     gene            4015..4674
                     /locus_tag="VI432_01995"
     CDS             4015..4674
                     /locus_tag="VI432_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4496897.1"
                     /translation="MQNRVFLFDMDGVLIDTERTWEKYGGDFLINLLGKDIADKVGST
                     LGLNVNDVYRIALENGFAMDKDEYLKIYDEQAKFIYSKSSLTLGMDELVKELHNLEFK
                     IGLVSASRRSWIDEVLPRLSFGDSFEHIISINDRPDLRNKPHPDGYIEAIRVLKGAPD
                     VSMVLEDSNPGIQAGKSAGAYTIGFRKNLLPAYEQEGADVYADEVEDVIRIVKLSTKK
                     E"
     gene            complement(4702..5337)
                     /locus_tag="VI432_02000"
     CDS             complement(4702..5337)
                     /locus_tag="VI432_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY4496898.1"
                     /translation="MQRAIVFASGTAKGGGSGFENLVNASRLGILQAEIVAVISNHGN
                     GGVAEKARRLGIQFLHMRAPFDGDRYWALFKGLGADFALLSGWLKKVNGLDPRKTINI
                     HPGPLPEFGGKGMHGHHVHEAVIKAFKRGEITHSAVSMHFVTKEYDMGPVFFRCPVPI
                     KHDDTPDTLGSRVNEMEHLWQPAITNLVMQGIISWDGKDPSSLKTIVLSSA"
     gene            complement(5689..6963)
                     /locus_tag="VI432_02005"
     CDS             complement(5689..6963)
                     /locus_tag="VI432_02005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4496899.1"
                     /translation="MDTNEKSFNGLGIAPGIMDIIAKLNLKIPTPIQEKSIPIAIEGK
                     DLIGIAQTGTGKTFAFGIPVIQLISQGNKLGLIIAPTRELALQINEALDKICFPLGIK
                     TATLIGGEPIYKQIRNLHRHPKIIIGTPGRIIDHMEQKTINLGRVNILVLDEADRMLD
                     MGFAPQLKRIQVGLSKERQTILFSATMPETIINMARSYMKLPIRVEIARQGSAAKDVT
                     QELFFVPQETKPMLLEKILKEYKGSVLIFSKTKFGVKKITSKIKSMRETAAEIHSNRS
                     LGQRKEALQGFKTGKYRILVATDIAARGIDVQNIELVINYDLPDNPEDYVHRIGRTGR
                     IGRTGHAISFARPNQRKDVQAIERLMRLVIPISKLTGMSEYIDNQNDFRQRVHKSHEG
                     RVQKTQSYDKNRSRQTTNYRHRRKYSSNRPKY"
     gene            complement(7058..7486)
                     /locus_tag="VI432_02010"
     CDS             complement(7058..7486)
                     /locus_tag="VI432_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496900.1"
                     /translation="MSPSIPGLGWKIANHQGFGKIEIRLGPDGELYIDGKKVVMHLSV
                     NQQGRNVIAGLKLFEELKGQPNLNAIIMDFLIENPHFIPKSFEAFNYIFFWGTTFSVY
                     KRLCIRCIVFKKNKWEKDFTWIQDNFGNKAPAAIIGNQTL"
     gene            7640..8617
                     /gene="obgE"
                     /locus_tag="VI432_02015"
     CDS             7640..8617
                     /gene="obgE"
                     /locus_tag="VI432_02015"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4496901.1"
                     /translation="MLIDDVKIRIIAGHGGKGAVAFQKNKMSLGPTGARGGNGGSIYA
                     EGQSDLSLLNQFRFKKELRAENGMDGRSQFRDGQDGKDLILKVPTGTVIYNLDDSRDI
                     SIERIGERVLLAKGGIGGRGNFHFRGPENTSPKESEPGKLGQEFNLRLELKLIADVGF
                     VGLPNVGKSSLLNYLTNAKSKVANYPFTTLEPNLGVYYELILADIPGLIEGSSTGKGL
                     GIKFLRHVERTNHLFHFVSSESENPQKDYEIIRGELGLYNKNLLQKPEYLFLTKSDLA
                     AKDEIDNKLKILRKINKDVVAISINEERSIKPVVKILNKIKDKKIIKSA"
     gene            8614..8832
                     /locus_tag="VI432_02020"
     CDS             8614..8832
                     /locus_tag="VI432_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY4496902.1"
                     /translation="MKIFVKAKPSSKKPGIIELEENKFIVAVSEPPIQGRANKAIIKT
                     LAEHFGTSSSRISLIAGFSSKEKIFELN"
     gene            8837..9397
                     /gene="msrA"
                     /locus_tag="VI432_02025"
     CDS             8837..9397
                     /gene="msrA"
                     /locus_tag="VI432_02025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005218260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4496903.1"
                     /translation="MDKTNLINSVYEIAVFGGGCFWCTEAIFSELTGVISVVSGYAGG
                     TKKNPSYEDVSGGTTGHAEVIKIEFSSKEINYEDLLTVFFATHDPTTLNRQGADVGTQ
                     YRSIILYTTAEQKDKAERFINELDKDGGDKIVTEVKPLEDFYEAEEYHKDYYARNIDK
                     PYCQVVISPKLNKLRSKFKELLKNKK"
     gene            complement(9398..9961)
                     /locus_tag="VI432_02030"
     CDS             complement(9398..9961)
                     /locus_tag="VI432_02030"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY4496904.1"
                     /translation="MKLLKRCAWPTPNDKLYIKYHDEEWGVPIYNDKKIYEFLVLEAF
                     QAGLSWRTILHKREGFRKAFANFNFNKVAQFNSRDIKNLLKNSDIIRNKLKIESAVEN
                     ARRFIDIRKEFGTFSKYMWSWVGKKPIKNNIHSIKDYKPYNKIATLWAKDLKKRGFKF
                     LGPTVLYAHMQAVGMVNDHMKNCFRNR"
     gene            9994..10989
                     /locus_tag="VI432_02035"
     CDS             9994..10989
                     /locus_tag="VI432_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY4496905.1"
                     /translation="MQSILYLIIILSAFSGFVLAFYIRHKKRSHEKMICLVGSNCDNV
                     IYSPYSRFFEIPVELLGMFYYGVIAVLYTIFIVFPSFVSPFLIFGVLAMSMAAFLFSL
                     YLTFIQAFALKQWCTWCLTSAGLCTIIFFSALFLSKFGFIDLLAQHRDVVTIFHVLGV
                     ALGLGAATITDIFFFKFLKDYKISEFEAEIMHTLSQIIWFALGVIVLSGIGLYLPESE
                     RLLASSKFLTKLVVVSIIIINGALLNLIVAPQLIKITFGGKHAHKSGELHRERKIAFA
                     LGAVSVASWYSAFILGSLKSIPISFPYLVGLYILLLFGAIIGSQFVERVFVKRED"
     gene            complement(11168..11521)
                     /locus_tag="VI432_02040"
     CDS             complement(11168..11521)
                     /locus_tag="VI432_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496906.1"
                     /translation="MPRESMDPSSLQIETIRSITGITEDVLNSVHDFFENITPNGGGR
                     LPKDETMANALRLFKKGKRNGAIIKLARQSRSVLNELEGPLNNDSAFQNRMAALIAAI
                     FLLIEAEEKIQKGLL"
     gene            complement(11564..>12182)
                     /gene="tpiA"
                     /locus_tag="VI432_02045"
     CDS             complement(11564..>12182)
                     /gene="tpiA"
                     /locus_tag="VI432_02045"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY4496907.1"
                     /translation="ESIKKTIKKSVLGAQNMFWGDLGAYTGEISWRQLKRLGVEYVIL
                     GHSERRKYLDETDELINKKVSAALRVGLNVILCVGEPLNIRRKGISIAKKFVKSQLLK
                     NLRSTNKLKSHLTNKLIIAYEPVWAIGTGKPDKPESAVEMSTFIKSIVMSQLSVLPPV
                     LYGGSVDAKNAEGFLSNDLIDGALVGGASLKSKEFQKIIKISSKF"
BASE COUNT         3284 a         2256 c         2590 g         4052 t
ORIGIN      
        1 cctccataga tccatcagcg ggtcaaggac tacgcggaca gattcatctt ccggaaggaa
       61 tgtgtcggtg atccgacgga agcctgcgcg gtcaagctct ttttcttctt ctggtgtgag
      121 ctcttgatag ttcggaatat aggcatagtg gctgttggcc accagtctgt ataagctctc
      181 ttccatgttt gcgccggtta cagaaattgc ggcgattttc ccggcctgga tgagcttgct
      241 aattgcgata cccatctgaa aagaagatcc tgcccctgcc attgtgagga atatttttcc
      301 cccttggtcg cagtgagttt tcagccaaag gcccgcatca acggtttttc ctccgttgca
      361 gtgacgtttg gtgtctacga gccaattgga aattggcgta ttcggattag gcttgaaatg
      421 cctttctcct ataataatct tggcccgaag actcggacta agagattgaa gtttccttcc
      481 tagcgtatgc gtttcagttg tcgtcatggg ttttgatcac ctagatgttc aattgtgaaa
      541 gagctttctt gcttcaatcg gcggatagat tgttccttcg ccaaccataa acaatatcat
      601 ataatcacta tgttttagct atgtcaattt gagttaaata aaaaatctcc accgttagtt
      661 aatggagagt accttgctct tcggtggagg ttttaggcgc aatatttgcg gattttattt
      721 tgcctcgtgg ttcgatgaat tttgcgccga attcaaggag acttggttta atcaattccc
      781 atagattttt gggaacgctc catgcctcgt tgataacctg cactcttgaa cttctatcgg
      841 agagaagtag aagatcctcg aattccgtac agacgaacat tcctccgaag acgcgcgaag
      901 taaggagcac tattctttgg ctatcgaata gatattttag ctctctttca agcgatcccc
      961 gattttcctc gctaaagaca agcttgccag ttgaatggac ttttatgtcg tttctcgcca
     1021 aaagcgcttt cattaggggc agattgtttg ctaaagatcc accagtgatc cgattcttct
     1081 taagggactg catatcgatc cctctaattg attcatagca ttgcgatcga gagatgtcaa
     1141 aacgataatc atctgccgtt taaacagaga ttcttagcgt ctgatgtagg caacttattg
     1201 catttatcgg cgagagtctc gttttctgtt acccaaagat taaggttgtc gccggactcc
     1261 gcataacata aatttctctg ttcttctttt atagtctcac agaactcagc aacagcttct
     1321 ggtgatccaa gcgctacttg aaccagaaat gacattgcgc ctttaataca ttcttcatat
     1381 gcggttttat ttatttgaag agagagcaag catttctccc cggctccttt aggggtctcg
     1441 tgtggtcctg ggatattgct gccggttcct cggaaacaaa tatttttata tttggaagcc
     1501 acttttaaac acaattcaaa agctttttta acatctcctc ctgtggcgac tagattattg
     1561 attcctttat atagataaca tgtatcctta tatttattat ttaatatttt gcacggataa
     1621 tctggatcgt tttcttgaaa ggcgagttct ttttgatgag agaatctagc gatttgtttt
     1681 gtttgaatat tctccataaa tgcgccggtg tagcatccgt tgggagattt tgtgcgatcg
     1741 caatattcaa gcgcgttatt taatttattt tctgttataa acataaaagc gtgtcccaag
     1801 ccgtgatagc ataacccttt tatcgctact ggattttttg aattattgat agattcttcg
     1861 cacgtttcat atattatatt tttcgtaaaa gtttccgggt cgtaagaaag ttctgcttga
     1921 ttgatatact cctcgactat gccgtgatac attccgctgt cgcatagtcc gcttgcgagt
     1981 tcaaatgctt ttgatattgt tttcgtttta gacaatgttt cccagccgag ataatgcata
     2041 aattcatgac accactgcaa tttgtatgaa tcgttgatgt ttctgattcc atcttctatt
     2101 tcttgcaatt gataggcgcg gcttgccgtt tcggcgagat tttttaaaca ctctcccttt
     2161 tcgcggtccg atatagattg atcagcgcat ttattatata aaaccgcgaa tgcttctttc
     2221 ggtgagccgg gcggagtcga atagattaaa tagccggcga cgccgattag aatagagatt
     2281 attaataaat tacgtttaga accgaacata tattggagtt tagcataaat ggaattattg
     2341 agttattgtt gtgccgcccg ttccatattc atggctctaa tgagtctggt aatccctatt
     2401 cctccgccga agcgagggaa aaaatcaaat gagagaaaat cttttagctc tttttcaacc
     2461 ctttcttttc cgaaaatatt atgcagggtt tcggcatatt tcccatcgct tatggtatga
     2521 aattgctcgt tcatctcgtt tttattaatg cttctttcgg cgcttccgat tgtttccatg
     2581 ccgtgtagga tgacgtctat tttatgtgcg taatccccat ttttcctcat gttccagaat
     2641 ggagatgttt tttgcggaaa catttctata aatgttacag cgctgaaatc atcttttatt
     2701 ttcatttcgt gatcatgagt taattcaacc gttccataat gatctgcaag ctcttcgtat
     2761 gtatgatgaa tagatttttc gttgaagccc attgctttgc agaggtcttt ttcaagtttt
     2821 cgcagatcgt tcattgttcc acgggattcg aattcaaaca tcggaaaaat tatattgtgc
     2881 ctctgcggga tcgggttggg ttcgtctctg aagcttgtgc ttacacagaa cactccttct
     2941 agtttaggat ttttaagaag ctcatattca agccacattt gtccagtttg gggcaagggc
     3001 cagatctcac cggcgtagtt gaatgtggcg atggttgatg ggtcttcgca ggcagccaat
     3061 atgcttaaac gatcttgggt atgcatttct gtgaatcccc tatctttgaa gaatgcgcgt
     3121 aatttattta ttgcttcgtc gaattctatg atattttttg ttgcgatata gttattaaac
     3181 attttggtta taagttttta gttggtaatg aatagtgtct gttgaagaat tatggcggac
     3241 ccttcgacaa tattcagggc ggggcgagaa tgcgagggta aattccctca cttaatttat
     3301 agatgcatgg ataaaaaaat acccccgatt tgcggggata ttttcctgaa gctttgaaaa
     3361 aaataaataa tttcttatgt ggctgattgt aatatagatt tttatttttg aatcgcatat
     3421 cacacaaact tctggaattt tagcatatat gaaatccagg tcaataagag agactgtgga
     3481 aatagataaa tagctaggtt atactttttg aagctactta tatgggggaa agaaaatgac
     3541 tggcactttg aaaaaagaaa tccatatgga tgacgtcatc ttgtttttta tgtgtcgact
     3601 cgcggaaggc gggcaagagg aaagaatagt tcttcctaca agcaagattc atcgatctct
     3661 tgctgattta ggtccgatgc tagaaaggct tgaggttttc gcaagattcc atcgagatcc
     3721 ggtagagact cattcccagc aggtgaatga agctgtagcg caccttgtac catttgccct
     3781 agagtcttct tggaagtatg gcgaaggaac aaggctggaa cttgctaaga ccgctgttcc
     3841 tcctaggatg gagaatctga agcaaaattt atctcctagt gttatcgatc ttcttgaggc
     3901 taatcttcaa aagtttcaag ctggactcgg atgacagaca acttacgttg ttaaatacaa
     3961 ctcctcgcag gtcgaggagt tttcatttta gagataaata tttatactta gcgcatgcag
     4021 aacagagttt ttctttttga tatggacggt gtactgattg atacggagcg gacgtgggag
     4081 aagtatggcg gtgattttct aataaatttg ttgggtaaag atatcgcgga taaagttggc
     4141 agcactttag gtttgaatgt caacgatgtt tatagaattg cattagagaa tggttttgct
     4201 atggataaag atgaatattt aaagatatat gacgaacaag ctaagtttat ttattctaaa
     4261 tctagtttaa ccctaggaat ggatgaattg gtaaaagagt tgcataattt ggaatttaaa
     4321 attggtttag tctccgcatc tagaaggtca tggattgatg aggttcttcc gaggctttct
     4381 ttcggtgatt cgtttgaaca tataatttct ataaacgata ggccggatct aagaaataaa
     4441 cctcatcctg atggatacat tgaagctata agagttttaa agggggcgcc agatgtatct
     4501 atggttttgg aagattctaa tcctggtatt caggcgggaa aatctgctgg agcatataca
     4561 ataggattta gaaaaaatct actaccagct tatgagcaag aaggggcaga tgtttatgcg
     4621 gatgaggtgg aagatgttat tagaatagtg aagttgtcta ctaaaaaaga atgacgccat
     4681 ttgatgtgac gtcgattttg tttaggcgct tgagaggacg atagttttaa ggcttgatgg
     4741 atcttttcca tcccaagata taatgccttg cataacaaga ttcgtgattg cgggctgcca
     4801 gagatgttcc atctcattta ccctagagcc tagagtatct ggcgtgtcat cgtgtttgat
     4861 gggcacaggg catcggaaga aaactggtcc catgtcgtat tctttcgtga cgaaatgcat
     4921 agagacagca gagtgagtaa tctcgcctcg cttgaaggct ttaattacgg cctcgtgtac
     4981 atggtgacca tgcatgcctt tgcctccgaa ttcaggaagc ggcccaggat ggatgttgat
     5041 ggtttttcga gggtccaggc catttacctt tttcagccag cccgacaaga gtgcaaaatc
     5101 agcccccaat cctttgaaga gagcccaata acgatcgccg tcgaatggag ctcgcatgtg
     5161 taggaattgg attcctagac gtcttgcttt ctctgctact ccgccgttcc catggttgga
     5221 tatgacggcg acgatttctg cttgcagaat acccaatctc gatgcattaa ctaggttctc
     5281 aaaaccagat ccgcctcctt tcgctgtgcc agaagcaaaa acgattgctc gttgcatatt
     5341 ctcacctgat tgttaaggaa gaagtgactt tctttatata tttattttat cagtcggtca
     5401 aatacagaga aaggacgctc ttcagagggc gtccttagtt ttgggatcta ggtccaatcc
     5461 gtttccggtc cgtggcggtg tgggtcgtga aagaccgaac aagttctgca taacggaaca
     5521 ggaatacctt cctggtctac cactatgggc acaattttgc ccgtaatgga atttgtcccg
     5581 gtgactattc ttgttggcgt tgtatcatcc cctttttaat agatgctgtt gtaattatag
     5641 cgtaatttta atctaaatta tagtcggggt tataggttgt tcgttgaatc aatatttagg
     5701 tctgtttgaa gaatattttc tcctatgtcg atagttcgta gtttgacggc ttctgttttt
     5761 atcgtaagat tgtgtttttt gaacgcgtcc ctcgtgagac ttgtggactc tttgtctaaa
     5821 gtcattttga ttgtctatat attcagacat gcctgttaat tttgatattg ggatgactaa
     5881 tctcataagt ctttctatag cttgaacgtc ttttctttga tttggtcttg caaaagagat
     5941 tgcgtgtccg gttctgccga ttcttccggt ccgtccgatt ctgtgaacat aatcttccgg
     6001 attgtccggc aaatcatagt ttataacaag ttcaatattc tgtacgtcta tgccgcgagc
     6061 ggcaatatcg gttgcgacaa gtattctata ttttccggtt ttaaaaccct gaagagcttc
     6121 ttttctctgc cccaaagatc tgtttgaatg gatctcggcg gctgtttcgc gcattgattt
     6181 aatcttagaa gttatttttt tgactccgaa tttggtttta ctgaatatta acaccgatcc
     6241 tttatattct tttaatattt tttctagaag cattggtttt gtttcttgcg gaacaaagaa
     6301 taattcttgt gttacgtctt tggcggcgct accttgtctt gctatctcaa ccctgatcgg
     6361 aagtttcata tatgaacgcg ccatatttat tatagtctcg ggcatagtcg ccgagaaaag
     6421 tatggtttgc ctttctttag ataatccgac ctgtattctt tttaattgag gggcaaaacc
     6481 catatcaagc attctatctg cttcatccaa cacaaggatg ttaactcttc caagatttat
     6541 agttttttgt tccatatgat caattattct tccaggtgtg ccgattatta tttttggatg
     6601 gcgatgcaga tttcttattt gtttataaat aggttcgccg ccgatcagtg tcgcggtttt
     6661 tataccaaga ggaaaacaaa ttttatccaa cgcctcgttt atctgcaaag ccagttctcg
     6721 agttggcgcg attataagcc ctaatttatt tccttgagat atgagttgta tgacggggat
     6781 gccgaaggca aaagtctttc ctgtacccgt ttgagctatg cctatcaagt cttttccctc
     6841 tatcgctata ggaattgatt tttcttgaat tggagtaggt atttttaagt tgagtttggc
     6901 aatgatatcc atgatgcctg gagctatgcc taagccatta aaactttttt catttgtgtc
     6961 catgtcgccg taagcataac agaggaatga attataagct atttatgaaa aagcggttat
     7021 caataaaaaa agagcagctg aaatctgctc gtgtgtttta gagagtctgg ttgccgatga
     7081 tggccgccgg agctttgttt ccgaaattgt cttgaatcca ggtgaaatct ttttcccatt
     7141 tatttttttt gaagacaatg catcggatac atagccgttt gtatacgctg aaggttgttc
     7201 cccagaagaa tatataattg aaagcctcaa aacttttagg gatgaagtgt gggttttcaa
     7261 tcagaaaatc catgattatg gcgttaagat tcggctggcc tttcaactct tcaaaaagtt
     7321 ttaagcctgc gatgacgttt ctcccctgct gatttaccga gagatgcata accacttttt
     7381 tgccgtctat ataaagttct ccgtctggac caagtcttat ctcaattttc ccaaatcctt
     7441 ggtgatttgc tattttccat cctaatcctg gtatggatgg gctcacgtta ctgtcgatta
     7501 cccttcgcgt caattggttt atcgatctag tcattgtctc atctccttca cagacttgtt
     7561 aaggtgcgcc ttaatgatta agcaacgagc cctttcgtgt caaatagcag atatccatta
     7621 aaaacattat actgatttta tgttgattga tgatgtaaaa ataaggatta ttgccggtca
     7681 cggcggaaag ggcgccgtcg cttttcagaa aaataaaatg agtttggggc cgactggcgc
     7741 acgcgggggc aacgggggca gtatctatgc cgaggggcag tcggatttaa gccttttgaa
     7801 ccagttccgt tttaagaaag aattaagggc tgaaaatgga atggatgggc gaagtcagtt
     7861 tagagacgga caggatggca aggacttgat tctaaaagtt cccacaggaa cagttattta
     7921 taatttagat gatagtagag atatctcgat tgaaaggatc ggtgaaaggg ttctccttgc
     7981 taaaggagga atcggtggca gaggaaattt tcattttcgc gggccggaga atacatcgcc
     8041 taaggaatcg gaaccaggca agcttggtca ggaatttaat ctgcgcttgg aattgaagct
     8101 tattgcagat gtcggatttg tcggccttcc taacgtaggg aaatccagct tgttaaatta
     8161 tctgactaat gctaagagca aagtggcaaa ttacccattt acgactcttg agcctaattt
     8221 gggagtgtat tatgaattaa tattggctga cataccagga ttaattgaag gatcttcgac
     8281 cggcaagggt cttgggataa aatttttgcg ccatgttgag cgtaccaatc atttatttca
     8341 ttttgtctca tccgaatcgg aaaatcctca aaaagattat gaaattatcc gcggagaact
     8401 cggtttatat aataaaaatt tgcttcaaaa accggaatat ttgttcttga cgaagagcga
     8461 cttagccgct aaagacgaga tagataataa attaaaaatc ctgcgaaaaa taaataaaga
     8521 tgttgttgct atatcaatta acgaagagag aagcattaaa ccagtagtta aaattctaaa
     8581 taaaataaaa gataagaaaa ttataaaatc ggcatgaaaa tttttgttaa agctaaacca
     8641 tcatctaaaa aacctgggat aattgagctt gaagaaaata aatttattgt tgcggtttca
     8701 gagcctccaa ttcaaggacg cgcgaataag gcgattataa aaacgcttgc ggagcatttt
     8761 ggcacgtctt cttctcggat atctcttatc gccgggtttt cttcaaaaga aaaaatattt
     8821 gaattaaact gattatatgg ataaaacgaa cttgataaac tcagtatatg aaatagcggt
     8881 tttcggcgga ggatgtttct ggtgcacaga ggcgattttt agtgaattaa cgggggtaat
     8941 atcggttgtc tcgggttatg ctggcggcac caagaaaaat ccttcctatg aagatgtctc
     9001 tggtggaacg acgggtcatg ctgaagttat caagattgaa tttagctcga aagaaataaa
     9061 ttatgaagat ttgctcactg tattttttgc cacacacgat ccgacaaccc tgaataggca
     9121 gggtgcggat gtcggtacgc agtataggtc aattatttta tataccaccg cagaacagaa
     9181 ggataaagcc gaaagattca tcaatgaatt agataaagat ggcggagata aaatagtaac
     9241 agaagttaag ccgctagaag atttttatga agcagaagaa tatcataaag attattatgc
     9301 gagaaatata gataagccat attgtcaggt agtgataagc ccaaaactaa ataaattgcg
     9361 ctctaaattt aaagaattgc ttaaaaacaa gaagtagtta tctgtttctg aaacaatttt
     9421 tcatatggtc gttaaccatt cctacggctt gcatatgagc atagagaaca gtcggcccta
     9481 aaaatttaaa tcccctcttt ttaaggtctt ttgcccataa ggttgcgatt ttattatatg
     9541 gtttatagtc tttaatagaa tggatattgt ttttgatcgg ttttttacca acccatgacc
     9601 acatatattt agaaaacgtt ccaaattctt ttctgatatc tataaacctt ctcgcgtttt
     9661 caacggcgga ttctattttc agtttgtttc ttattatatc ggaatttttg agcaaatttt
     9721 taatatcacg tgagttaaat tgtgcaactt tattaaaatt gaagttggca aaagctttac
     9781 ggaagccttc tcgcttgtgg aggatcgtcc gccatgagag accggcttgg aatgcttcta
     9841 aaactagaaa ttcatatatt tttttatcgt tataaattgg cacgccccat tcttcgtcgt
     9901 gatatttaat atataattta tcgttcggcg tcggccatgc gcatcttttt aacagcttca
     9961 taatatgaaa gtatatctgt tatacttagc taaatgcaat ctattttata tttaatcatc
    10021 atattgtccg cttttagcgg gtttgttctg gctttttata tccgtcacaa aaaaagatct
    10081 catgagaaaa tgatctgtct tgtcggatcg aattgcgata atgtgattta tagtccgtat
    10141 tccagatttt ttgaaatacc agttgagcta cttggaatgt tttattatgg agttattgct
    10201 gttttgtata ctatttttat agtatttcct tcattcgtct cgccgttttt gatttttgga
    10261 gttctagcga tgagcatggc tgcgtttctt ttttcgttat atcttacttt catccaagct
    10321 tttgcgctaa aacagtggtg cacttggtgt cttacttccg ccggactttg tacgattata
    10381 ttttttagcg ccttattcct ttctaagttt ggatttatag atttacttgc gcagcacaga
    10441 gatgttgtaa ctatttttca tgttcttggt gttgctctcg gtttgggggc cgcgacgata
    10501 acagacattt tcttttttaa atttttgaaa gattataaga tttctgaatt tgaagcggaa
    10561 ataatgcata cgctttcaca aattatctgg tttgctttgg gggttatagt tctgagcgga
    10621 ataggattat atcttccgga atcagagagg ctgcttgcgt cgtctaaatt tttaacgaaa
    10681 cttgtggttg tgagcattat tattataaac ggagcactat tgaatttgat tgtcgctcca
    10741 cagcttatca aaattacttt cggaggcaag catgctcata aatccgggga attgcatcgt
    10801 gaaagaaaga tagcatttgc ccttggagcc gtttcggtcg cgtcttggta ttcagctttt
    10861 attttagggt ctctaaagag tattcccatc tcattcccat atttggtcgg cttgtatata
    10921 ttgttgctct tcggagctat aatcggcagt cagtttgtag agagggtttt cgtaaagaga
    10981 gaagattagt taaccaaaaa cccccgccga agcgggggca aacaaataat aattgggaca
    11041 atccttagcc gatatcatcg ttaagggctg ttcatcactc acttacaaag aagatagcat
    11101 atctaaatta aaaagtcaat ttctgaaata ataaatctcg cattgtacgc gagattggat
    11161 gtgactttta aaggaggcct ttttggattt tttcttcagc ctcgatgaga agaaagattg
    11221 ctgcaatgag cgcggccatt ctgttctgga aggccgaatc gttattgaga gggccttcaa
    11281 gctcgttgag aacactacga ctttgtcggg cgagttttat aatcgcacca ttccgttttc
    11341 ctttcttgaa aagacgaagt gcgtttgcca ttgtttcgtc tttaggtaat cgtcccccgc
    11401 cattaggggt gatattctcg aagaaatcgt gaacgctgtt taaaacatcc tcagtgattc
    11461 cagtaatcga tcttattgtt tctatttgta aggaacttgg atccatgctt tctctaggca
    11521 tgtaaaatga agagtaaaac acataggcgt atttgtcaac gttttaaaat ttggaagaga
    11581 tttttattat tttttggaat tctttagatt ttagacttgc accacccaca agcgccccat
    11641 caataagatc gttgctcaga aaaccttctg cgtttttagc atctaccgat ccgccataca
    11701 gcacgggcgg taaaactgac aattggctca tgacaattga ctttataaat gtagacattt
    11761 cgacagcgga ttctggcttg tctggttttc ctgtgccgat tgcccagacg ggttcataag
    11821 caataattag cttattagtg agatggcttt ttaacttgtt agtgcttcgt aaatttttta
    11881 gaagttgtga ttttacgaat tttttcgcga ttgaaatgcc tttacggcgg atatttagag
    11941 gttctccgac acataaaatt acgtttaagc cgacccgcag cgcagcggac acctttttat
    12001 taattagctc atctgtttcg tctaaatatt tacgcctctc ggagtgcccg agtataacat
    12061 attctacacc cagccttttt aactggcgcc acgatatctc ccctgtgtat gctcctaaat
    12121 cgccccagaa catattttga gcgccaagca cagatttctt aatagttttt ttaattgatt
    12181 ct
//
LOCUS       DATDNZ010000051         5048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1707222, whole genome shotgun sequence.
ACCESSION   DATDNZ010000051 DATDNZ010000000
VERSION     DATDNZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707222"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1433)
                     /locus_tag="VI432_02050"
     CDS             complement(222..1433)
                     /locus_tag="VI432_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4496908.1"
                     /translation="MLYFYEAVDRNGKQVKSSIEALDEREVVVYLEGKNLVPVKIDAQ
                     KTKKAISLSTNIFERITPTDRIFLVRNLSTAIKAGLSLSESLDILIADTKKEVLKNIL
                     IQAKNNIQSGKTLSETFAAHSKEFPPIFEGLLKAGETSSQLDKSLDELNKHLSKEYAL
                     VKKIKSAMVYPMLLLAASLGVIIFLLTFLLPRLAKSFAQSGAELPLITKILLSISDAI
                     TYSPILSISIFAAIIAGFIYLRRSKRGKRMWAFMLFKTPVVRELIKGVALVRFTRTLG
                     SLLGGATPIIEAMRICSESVGNTYYKKSIDDAVEGIRGGVPLSKTLQKYPDLFPPLLT
                     GLLAVGERTGNLEYVLGTFSNFYDDEIENKLKDLTTLFEPVLLIIMGLTVGAIAISVL
                     LPIYKLVGEFV"
     gene            complement(1477..1863)
                     /locus_tag="VI432_02055"
     CDS             complement(1477..1863)
                     /locus_tag="VI432_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4496909.1"
                     /translation="MRSIKKRFRFRDFRVYKEARVFSGVLKIDSKKFMPREEQFILLS
                     QLWRALDSIILNIAEGADRGTDKDFAHFLNNAHTSLNEVVACLDIALDNGYISIKGHD
                     VYLDRAENLAEQLTAFRRKLLDEPTK"
     gene            complement(1919..3640)
                     /locus_tag="VI432_02060"
     CDS             complement(1919..3640)
                     /locus_tag="VI432_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY4496910.1"
                     /translation="MFLTTERFKSILVNAGVIDDKTFDEAYREAEKAGQPLEEILIGR
                     GEVPEYYLSELLGKSLNIPTINLQGVSIDRSVLERIPESYAQSKQLVLFDFNEEVGAK
                     VAMLDPLDYGTIEFLRVKLGAPVEAYITAKSSLNGALKQYKEKIGTEFSKVIAENVGR
                     SLSITGETDLSKMAESVPIISILKNIMEYAIVLGASDIHFEPFFKNLIIRYRVDGVLR
                     EIFNLPKQIEPILIARIKILANLQIDEHRIPQDGRFKFEMSEKEYIDVRVNVAPVLYG
                     EKGEMRLLRSTARPLTLEELGLSSDKIKIVQEGIKKPHGMILVTGPTGSGKTTTLYAI
                     LNILNTPKVNITTIEDPIEYEVAGINQIQVNQKAGITFANGLRSILRQDPNIIMIGEI
                     RDEETVNIGIHAALTGHLLLSSLHTNDAPSAIPRLLDMKAPPFILASTINMVVAQRLV
                     RKICLACIESYEASTEINKLVEAQLVLIGHETGENIKNKLPKTLYRGKGCNVCGDSGY
                     KGQIALFEVLKVSDGVKELALKSAPSDEIRELAIKEGMDTLFQDGLEKAKAGVTTIEE
                     VLRVIQE"
     gene            complement(3668..4030)
                     /locus_tag="VI432_02065"
     CDS             complement(3668..4030)
                     /locus_tag="VI432_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4496911.1"
                     /translation="MKIQRFTDLEAWKRAHKLALAIYKITKNFPQEEKFSLVDQMRRC
                     AISISSNIAEGFSRQSKKEKNQFYFIAKGSVTELQNQLLISKDIGYLSSADFNSVAEQ
                     AIYVHKLINGLIKSSASY"
     gene            complement(4070..4753)
                     /locus_tag="VI432_02070"
     CDS             complement(4070..4753)
                     /locus_tag="VI432_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496912.1"
                     /translation="MVQFLLDPFITWLPIIFTWAVLFLAIFAYVFKTHNLFQITWFSW
                     INYKFLSIATIIFIFSYTIILTIAQYYVWANSGSFTGLFLNSPLSPEIPPSFVTNLLP
                     WVFESPLGYFFFYSWGRFWINALLSIGMALVFWLFLRALKKNNERFFYPSETELGFLC
                     ALIVGWPSFLVFIPLVFLSVIIVSIIRAIVYREAYTTLGYPFILGAFLSLIWGGWLID
                     LLNLGVFKI"
     gene            complement(4815..>5048)
                     /locus_tag="VI432_02075"
     CDS             complement(4815..>5048)
                     /locus_tag="VI432_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496913.1"
                     /translation="VATDTAPINTDLVYRYVCQNTSTSPAPNFVFEFNATLESNEYAG
                     AAATGRDYTDGGDHDTLYEVGTSLRLLGGSNTH"
BASE COUNT         1572 a         1130 c          807 g         1539 t
ORIGIN      
        1 tcttttacaa ctccgccata gaatccggca accatagaaa ccatgattaa taataatcct
       61 attgcaatca caagctccag cagagtgaat ccatctctcc gagctggatt cactctgaca
      121 attaacatct ggccaaaaac atctgacttt aagtttttat ttttcttatc catattttta
      181 gttaagtgtt aattgtttat tgttaagtgt tagcgtcatc gctacacaaa ttctccaacc
      241 agcttatata tcggcagtaa tactgaaatc gcaatcgcgc cgactgtaag ccccataatg
      301 attaaaagca cgggctcaaa aagagtcgtt aaatctttta atttattctc tatttcatca
      361 tcataaaaat tagaaaatgt tcctaaaaca tattccaaat tgccagttct ctctccaaca
      421 gccaacaggc ccgttaaaag aggggggaac aaatcgggat atttctgcag agttttagaa
      481 agaggaactc cgccccttat tccttcaacc gcatcatcaa tcgatttttt ataataggta
      541 ttaccgaccg attcagaaca tattctcatc gcctcaatta taggagtcgc tcctccgagc
      601 aagcttccca aggttctcgt aaatctcaca agcgcaacgc cttttataag ctcgcgcaca
      661 acaggagtct taaataacat aaatgcccac attctctttc ccctcttgct tcgtcttaaa
      721 tatataaaac cggcgatgat tgcagcaaat attgagatgc tcaatatcgg gctataagtt
      781 atggcgtcgc tgatactaag taatattttt gtgattagcg gaagttcagc tccgctttga
      841 gcaaagcttt tcgcaagccg aggcaataaa aacgtcaaaa gaaaaattat cacaccaaga
      901 cttgccgcaa ggagaagcat cggatagacc atcgctgact ttatcttttt aactagcgca
      961 tactccttgc ttaaatgttt atttaattca tctaaagatt tatccaactg actggaagtc
     1021 tctcccgcct tcaatagccc ctcaaaaata ggaggaaatt ctttagaatg agcggcaaat
     1081 gtttctgata atgtttttcc gctctgaata ttattctttg cttgaataag aatattttta
     1141 agaacttctt tcttggtgtc ggctatcaaa atatccagcg actcgctcag gctcaatccg
     1201 gctttaatcg cagtagatag attacgaact aaaaagatcc tatctgtagg agtaattctc
     1261 tcaaaaatat ttgtggataa acttatagct tttttagttt tttgagcatc tatctttacc
     1321 ggaacaagat tcttgccctc caaataaaca acaacttcgc gctcatcaag cgcctcaatg
     1381 ctggacttca cttgtttgcc gtttctatct acagcctcat aaaaataaag catagcttta
     1441 ttttacactt aacaattaac aattaacact tgacctttat ttggttggtt catcgagaag
     1501 ttttcttcta aatgcagtaa gctgttcagc aagattttct gctctatcta aatatacatc
     1561 atgaccctta atactgatat atccattatc taatgcgata tctaaacaag caacaacttc
     1621 attgagggat gtgtgagcat tatttaaaaa atgagcaaaa tctttatcgg ttccgcgatc
     1681 agcgccctct gcaatattca aaataataga atctaatgcc ctccataact gtgataataa
     1741 tataaattgt tcctccctgg gcataaactt tttggaatct atctttaaga caccagagaa
     1801 aactctcgcc tctttataaa ctctaaagtc tctgaatcta aatcttttct ttatcgatcg
     1861 catcgttaaa tgttaggtgt cagatgttaa gtgtcgtgta attcaaggat aggctagcct
     1921 attcttgaat tacacgcagc acttcctcaa tcgtagtcac tccagcctta gccttttcta
     1981 atccgtcttg aaataaggtg tccattccct ctttaatcgc taattccctt atctcatcgg
     2041 aaggagcgct cttaagcgcc aactctttta cgccgtctga aacctttaac acctcaaata
     2101 gcgctatctg ccctttataa ccgctgtccc cgcatacgtt gcatcccttg cctcgatata
     2161 aagtcttcgg caatttgttt ttaatatttt cacccgtttc atggcctatt aaaaccagct
     2221 gcgcctcaac aagcttattt atctcggtgg aggcttcata tgattcgatg caggcaagac
     2281 aaatctttcg gaccagcctc tgagccacca ccatattgat ggtagacgca agaatgaatg
     2341 gaggagcttt catatccaag agtctcggaa ttgccgatgg cgcgtcattt gtgtgtagcg
     2401 atgaaagcaa taagtgtccg gtcagggccg cgtgtatgcc tatattcacc gtttcctcgt
     2461 ctcttatttc accgatcatg attatattcg ggtcctgcct caatattgat cgtaggccat
     2521 tcgcaaaagt gatgcccgct ttctgattca cctgaatctg attgattccc gcaacctcat
     2581 actcaatagg gtcttcaatc gtcgtaatgt ttactttcgg agtatttaaa atattcaaaa
     2641 tagcataaag ggttgttgtc ttgccgcttc ctgtcgggcc agtaaccaaa atcattccat
     2701 gaggcttttt aattccctcc tgcactattt ttattttgtc tgaagacaat ccaagctctt
     2761 cgagagtaag cggtctggca gtgctcctca aaagcctcat ctcacccttt tctccatata
     2821 aaaccggcgc gacattcacc ctcacatcaa tatattcttt ctcactcatc tcaaatttaa
     2881 atcgtccgtc ttgcggaatc ctatgctcgt ctatctgcaa attagccaaa attttaattc
     2941 gagctataag tatcggctca atctgctttg gcaagttaaa aatctccctt aaaactccgt
     3001 ctactctata cctgataatc aggtttttaa agaaaggttc aaaatgaata tcggatgcgc
     3061 caagcacaat ggcatattcc ataatatttt taagtattga aataataggc acagattccg
     3121 ccatcttaga taagtccgtc tctcctgtaa tagagagaga tcttcctacg ttctccgcaa
     3181 tcaccttgct gaactccgtc ccgatttttt ccttatactg ttttaaggct ccgttcagac
     3241 tggatttggc agtaatataa gcctctacag gggctccaag ttttacgcgt aaaaattcaa
     3301 tcgtgccata gtccagcgga tcaagcatcg caactttcgc gccaacctct tcattgaaat
     3361 caaaaagcac caattgttta gattgagcat aactttccgg gatcctctcc aaaacactcc
     3421 gatcaatcga aactccttgc aaatttattg tcggaatatt caatgatttg ccgagaagtt
     3481 cactcaaata atattccggc acctcacctc tacctatcaa aatttcttcc aacggctggc
     3541 cagccttttc cgcctctcta taagcctcgt caaaggtttt atcatcaata actccggcgt
     3601 taacgaggat tgatttaaat cgttcagtgg tcagaaacat tagtattaag tataaagtat
     3661 ttggtattta gtatgaagcg gagcttttaa taagaccatt tataagctta tgaacatata
     3721 ttgcctgttc ggcaacgcta ttaaaatcgg cactcgataa ataacctatg tctttagata
     3781 ttaaaagttg attttgtagc tcggttacag atcctttagc aataaaataa aattgattct
     3841 tctctttttt actttgccta gaaaaaccct cggcaatatt agaactaata gaaatagcgc
     3901 atctacgcat ctgatctact aatgaaaact tttcttcttg tgggaaattc ttagtgatct
     3961 tataaatagc aagtgctaat ttatgagccc gcttccaagc ctctagatca gtaaatcttt
     4021 gtattttcat atacttaata ccttatactt aatactagat actattctct catattttaa
     4081 acactcccaa attgagcaag tctatcaacc atccgcccca gatcaagctt aaaaacgctc
     4141 cgagtataaa ggggtatccc aaagttgtat aagcttctct atatactatc gcgcgaatta
     4201 ttgaaactat aataacagac aagaaaacca acggaataaa aactagaaag ctcggccatc
     4261 cgacaataag cgcgcataaa aatcccaatt cagtctcact cggataaaaa aatctttcgt
     4321 tatttttctt caacgcgcgc aaaaatagcc aaaaaaccaa agccatgcca atagacaaaa
     4381 gcgcgtttat ccaaaatctg ccccaggaat aaaaaaagaa atatccaaga ggactctcaa
     4441 atacccaagg caacaaatta gttacaaaag acgggggtat ctccggacta agcggtgagt
     4501 ttaaaaataa tcccgtaaaa gaaccgctat tcgcccaaac ataatattga gcgattgtaa
     4561 gaatgatagt atatgaaaaa ataaaaatta ttgttgcgat actcaaaaat ttatagttta
     4621 tccaagaaaa ccaggtaatc tggaataaat tatgggtttt aaaaacataa gcaaatatag
     4681 ccagaaataa aaccgcccaa gtaaatatta tcggtagcca agttataaac ggatctaata
     4741 aaaattgaac catttttcta caaatttaaa accgaccatc atggtgagtt ttaattatac
     4801 aataaaacta gcttttagtg agtattactg ccacccaata gacgtaaact tgtgccaacc
     4861 tcatacaaag tgtcatgatc tcctccatca gtataatctc tacccgttgc agctgcgcca
     4921 gcgtattcat tactctccaa tgtcgcatta aattcaaaaa caaaattagg agcaggacta
     4981 gttgaagtat tctgacacac atatctataa acaaggtcgg tatttattgg agccgtatca
     5041 gtagcaac
//
LOCUS       DATDNZ010000052         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2751456, whole genome shotgun sequence.
ACCESSION   DATDNZ010000052 DATDNZ010000000
VERSION     DATDNZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751456"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VI432_02080"
     CDS             complement(<1..1549)
                     /locus_tag="VI432_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY4496914.1"
                     /translation="MARRNRKNKSRGEGYTLNEDAKTSIIAISFWGIAVIFLLSSFNM
                     AGPIGELIFKYMTDLLGIGYYLIPFTLIIIGLIFATGENKKIFSLTIIGSLIFIMSAL
                     GLIDVIYPEQGGLIGKFFGLLEVPFGKIASVIIHITILVSSIVLTINTPFKIKFKKGD
                     KSSKLLPTNPEVILPQEYQEPEREITIPSEQKSKEKTLPLKNKKDETEPEKEKMEIKH
                     PPSFKNNYIAPPINLLRTSNEKPTVGDLRANANIIKRTLESFGVPVEMGEITIGPKVT
                     RYTLKPAEGIKLARITALNQDLALALAAHPIRIEAPIPGKALVGIEIPNKTASIVRLG
                     NLISYKDFAEAGQIGFVLGRDVSGEPVFADLAKMPHLLIAGSTGSGKSITIQTLLISL
                     LYKNSPESMRLLLIDPKRVELSIYDGIPHLVTPVITEGKKSVAALRFAIEEMDRRYGA
                     LLKAGQRDILSYNEGRKEDIMPYLVIVIDEMADLMATFGREIESYIVRLAQMARATGI
                     HLVVSTQR"
     gene            complement(1582..>2185)
                     /locus_tag="VI432_02085"
     CDS             complement(1582..>2185)
                     /locus_tag="VI432_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4496915.1"
                     /translation="LKELRKYDGIIVPGGFGGRGVEGKIAAIKYCRENKIPFFGLCYG
                     LQLATIEYARSILKLKDANTTEVSPKTKHPVIDILPEQKENIAAKNYGATMRLGAYPA
                     RLKKGTIARSAYKKESISERHRHRFEVNPEYIERLERAGLIFSGSSPDRRLMEIIELP
                     KSKHPFFLGTQFHPEFKSKPIISHPLYSAFIKAALNAKIK"
BASE COUNT          556 a          445 c          430 g          754 t
ORIGIN      
        1 gtctttgtgt tgagaccact aagtgtatgc ctgttgctct tgccatttgc gctaagcgga
       61 caatgtatga ttctatttcc cttccaaaag ttgccattag atcagccatt tcatctatca
      121 caatcacaag atatggcata atgtcttctt ttcgcccttc gttatagctt aaaatatctc
      181 tctgccccgc ttttagaagg gccccatatc ttcgatccat ttcttcaata gcgaatctaa
      241 gcgctgcaac tgatttttta ccttctgtta taaccggagt tactaaatgt gggatgccat
      301 cataaattga tagttcaact ctttttgggt caatcaggag aagtctcata gactctggag
      361 agtttttata taagagagat ataagtaggg tctgaatggt gattgatttt ccgctacccg
      421 ttgatccagc tataagcaag tggggcattt ttgccagatc cgcaaaaacc ggttcgcctg
      481 acacgtctcg tccaagcaca aaaccgattt gtccagcttc tgcgaaatct ttataactaa
      541 tcaaatttcc tagccttacg atactcgcgg ttttatttgg tatttctatg ccaaccagcg
      601 ccttacccgg gatcggagcc tctattctta ttgggtgcgc ggcgagcgct aaggccaagt
      661 cttggtttag agccgtaatc ctggcaagct ttatgccttc agccggcttt aatgtgtatc
      721 tagtaacttt tggacctata gttatttcac ccatttcaac tggaacgccg aaactctcta
      781 atgtacgctt gatgatattt gcattagccc ttaaatcacc caccgtcggt ttctcgttgg
      841 atgtgcgaag aagattgatt ggcggagcaa tataattatt tttaaaagat ggagggtgtt
      901 taatctccat tttctctttt tcgggttcag tctcgtcctt tttatttttt agtggtagag
      961 ttttttcttt tgatttttgc tcgctcggta ttgttatctc tcgttcaggc tcttgatatt
     1021 cttgtggcag aataacttca ggatttgtcg gtaacaactt tgatgattta tctcctttct
     1081 taaacttaat tttaaaagga gtattgattg tgagcacgat tgaggagact aaaattgtta
     1141 tgtgaattat cacagatgct attttgccga atggaacttc gagcaggcca aagaatttac
     1201 caattaatcc tccttgttca ggatagataa catctatcaa tcctagagcc gacattatga
     1261 aaataaggct gcctattatt gtaaggctga atattttttt attttctcct gtagcgaata
     1321 ttaagccgat tataataaga gtaaacggga tcagatagta gccgattccc aagagatccg
     1381 tcatatattt aaatataagt tcaccgatcg gtccggccat attaaaacta gacaaaagga
     1441 agataacagc tatcccccaa aaggagatgg ctattatgct tgtttttgca tcttcattga
     1501 gggtataacc ctctccgcga gatttatttt tacggtttct tctagccatt tatatcaaga
     1561 ctcactaaaa aagattttgg attatttaat cttagcattt agcgcggctt taataaatgc
     1621 gctgtataaa ggatgggaga ttatcggttt tgatttgaat tctgggtgga actgggttcc
     1681 taggaaaaag gggtgtttgg attttggcaa ttctattatt tccattagtc gtctatcggg
     1741 tgatgagccg gaaaagataa ggccagcgcg ttcaagccgt tcaatatatt caggatttac
     1801 ttcgaatcta tgcctatgcc tttcagaaat tgactctttt ttataagccg atctggctat
     1861 cgtgcccttt tttaatcttg ccggataagc tcccaatctc atagttgctc catagttctt
     1921 tgcggcaata ttctcttttt gttctggcag aatatctatc accgggtgtt ttgttttcgg
     1981 gctaacctcg gttgtgttag cgtctttaag ttttaatatc gagcgcgcat attcaatcgt
     2041 cgctagttgt aacccgtaac ataaaccgaa gaaagggatc ttattctctc ggcaatattt
     2101 gattgcggct attttccctt ctactcctct cccgccgaat cccccgggta ctataatgcc
     2161 atcatacttt ctaagctctt ttaag
//
LOCUS       DATDNZ010000053         5151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2756326, whole genome shotgun sequence.
ACCESSION   DATDNZ010000053 DATDNZ010000000
VERSION     DATDNZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756326"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="topA"
                     /locus_tag="VI432_02090"
     CDS             complement(<1..1028)
                     /gene="topA"
                     /locus_tag="VI432_02090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY4496916.1"
                     /translation="MKLVIVESPTKAKTISKFLGKDFKIESSYGHVRDLPKGKLGVDV
                     DNNFEPKYVTPRKNQKTVTALKKLSAKSDSVILATDEDREGEAIAWHLANALGLNENK
                     DKAQRIVFHEITKTAIEKALENPRNINLDMVNAQQARRVLDRLVGYKLSPFLWKKVAS
                     GLSAGRVQSVALRLIVEREEEIRKFNPQEYWTIEAELEKDKRFIAELTKIDEKPIEKL
                     EINSEESAKKISVELKNGEFKISNVIKKQLTRNPYPPFMTSTLQQEASKRLGLTAKKT
                     MFIAQGLYEKGYITYMRTDSLNLSEESLLKAQTWIEKNIGVEYAKNSPRRFKSKSKLA
                     QEAHEAIRP"
     gene            complement(1077..1940)
                     /gene="dprA"
                     /locus_tag="VI432_02095"
     CDS             complement(1077..1940)
                     /gene="dprA"
                     /locus_tag="VI432_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY4496917.1"
                     /translation="MKEDIKTITIDSPFYPPLLKEISDPPQKLYIKGNLSINEPTIAI
                     VGTRKATQEGIKTAEIFAEKLGLYGVTIISGLAMGIDAAAHRGALNSGTKTIAVLGCG
                     IDKIYPAQNESIAQKILESGGGIISEYPPGSPSYKHQFLERNRIVSGLSLGTIIIEAP
                     ERSGSIRTANSAAEQGRSIFVIPGPINHPNFKGSHNLIREGATLATSIEEIMQDLNIE
                     IKQEKEMETTSNLNETQYAIYNCIKKSGRSISIDKISEGTKLHPKIINQNITDIVITG
                     LIKESGGLYNI"
     gene            1992..2516
                     /gene="pilO"
                     /locus_tag="VI432_02100"
     CDS             1992..2516
                     /gene="pilO"
                     /locus_tag="VI432_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY4496918.1"
                     /translation="MVNFRNRFILQVFATFIILIVLISIMVYLGIQIKESASTIYSTR
                     QDLRDKTHNISELSQLRLAAKEAEPFLEKLEAAIPSKDELFSFADDLKEIARMNDLGL
                     NFKFVGEAIISGGLGSQKFEANAQGDYRDITFFIRELERSGYITNVLDVNILKQGSLF
                     NALLIGEVYFNAQA"
     gene            2503..2700
                     /locus_tag="VI432_02105"
     CDS             2503..2700
                     /locus_tag="VI432_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496919.1"
                     /translation="MLKRKFHKIELILLVSATLIFIALVSGIIYALGFISGNVIHALS
                     GELGSPSSVSFNLDRYNELDL"
     gene            2769..2840
                     /locus_tag="VI432_02110"
     tRNA            2769..2840
                     /locus_tag="VI432_02110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2801..2803,aa:Ile,seq:gat)
     gene            2944..3018
                     /locus_tag="VI432_02115"
     tRNA            2944..3018
                     /locus_tag="VI432_02115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2976..2978,aa:Val,seq:gac)
     gene            3142..3702
                     /locus_tag="VI432_02120"
     CDS             3142..3702
                     /locus_tag="VI432_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496920.1"
                     /translation="MKELIESPHVDRDSLKALFAVSSALERMIPRLPSAEQHRRGGYF
                     CLMPRERRGHSVGTPILVIQVGEVDDPEKRHKYYKFAVEKAGRLHAYADTDSHKSSWQ
                     SRHPSTDRWGGAILTNKWIFSFSGLPELADEAIMLFAAITLGFMERADADAIARISDN
                     QLYFKLFVQRGWSDHNQTVWDPARMT"
     gene            3783..4556
                     /locus_tag="VI432_02125"
     CDS             3783..4556
                     /locus_tag="VI432_02125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4496921.1"
                     /translation="MLEPKIIYENENVLAINKPAGLMIHGVGGKHFREGTQREEETLV
                     DWIKENRPEIVGVGDKDLDGNLPQNDRPGIVHRLDRYTSGVMLIAKTNEYFQYLKSLF
                     QSHQIKKTYLALVVGWPDEDEGIINKPIGIKDGSVKRTTFIKKAKMVREALTEYKVIR
                     KFLFIEDDVEHKYALVELRPQTGRTHQIRVHLASIGHPVVGDELYGKKDERFSLNRYL
                     LHADSIEFPKEEGDRVRFSADISDEFKEVLGKLTLSNLL"
     gene            4614..4994
                     /locus_tag="VI432_02130"
     CDS             4614..4994
                     /locus_tag="VI432_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY4496922.1"
                     /translation="MISEDILNKIRPGVKIRVSERIPAYITSAGGKGKKKDDKAASKG
                     GERISSFEGLVLARKHGGEIGATFTVRATVAGVGMEKIYPINSPLLNKVEILSQPKKT
                     HRAKLYYLRNISAKKTRKKIGAEK"
BASE COUNT         1410 a          940 c         1121 g         1680 t
ORIGIN      
        1 ggtcttatcg cttcgtgagc ttcttgagcc agttttgact tgctcttaaa tctacgtgga
       61 gaatttttag catattcaac gccaatattt ttttctatcc aagtttgcgc ttttaataga
      121 gattcttcgg atagatttaa agaatcagtt ctcatatatg ttatgtaacc cttctcatat
      181 aatccttgag ctataaacat ggtttttttt gcagttagcc ccagcctttt tgatgcttct
      241 tgttgaagcg tgcttgtcat aaatggtgga tacgggtttc tagttagttg tttttttata
      301 acgttagaga ttttgaattc tccatttttt aattcaacgc ttattttctt tgccgattct
      361 tctgaattta tttcaagctt ttctattggc ttctcatcta ttttcgtaag ttcggctata
      421 aaacgcttgt ctttttctaa ttctgcttca atcgtccagt attcttgagg attgaattta
      481 cgtatttctt cttctctctc cacaataagt cttagagcta cagattggac tctgccggca
      541 gaaagacccg aagccacctt cttccataaa aatggagaaa gtttgtatcc gacaagcctg
      601 tctaagacgc gtctggcttg ttgcgcgttt accatgtcta gatttatgtt tcttggattt
      661 tctaaggctt tttctatggc tgtttttgtg atttcgtgaa aaactatcct ttgcgcttta
      721 tctttgtttt cgttcagtcc taatgcgttt gccaggtgcc acgcaatagc ttctccttct
      781 ctatcttcgt ctgtcgcgag tattacagaa tccgatttag cggataattt tttaagggct
      841 gtaacggttt tctgattctt gcgcggagtt acgtattttg gttcaaaatt gttatctacg
      901 tcaactccga gcttgccttt tggaaggtct ctcacgtgtc cataggaaga ttctatctta
      961 aaatcttttc ctaaaaattt tgaaattgtt ttagctttgg taggggactc tacgataact
     1021 aatttcattc tctcgtttaa ttaaaattaa taaatagttt tgaccgttga tttatcttat
     1081 atattgtata atcctccaga ttctttaatt aatccagtaa taactatgtc ggttatattt
     1141 tggttgatta ttttcgggtg aagtttagtg ccttctgata ttttgtcaat gcttatggat
     1201 ctgccagact tttttatgca gttatatatc gcatattgag tttcgttgag attggatgtt
     1261 gtttccattt ctttttcttg tttaatctct atgtttaagt cttgcataat ttcttcgatt
     1321 gacgttgcaa gtgtagcacc ctctcttatc aaattatgcg atcctttgaa atttggatga
     1381 ttgatcggtc cagggatcac gaatatgctt cttccttgtt cggccgccga attggcggtt
     1441 cttatggatc cggacctctc cggcgcttct attattatag ttcctaggga tagtccgctc
     1501 actatcctgt tccgttctaa aaattgatgt ttatatgaag gagagccggg tggatattcc
     1561 gatattatcc ctccgcccga ttctaagatt ttttgcgcta tggattcatt ttgcgcgggg
     1621 tatattttat caattccaca acctagaacc gcgattgtct ttgtgccgga gttgagagct
     1681 ccgcgatgag ccgctgcgtc tatccccata gcaaggccgc ttattattgt cacgccatat
     1741 aatcccaatt tctcagcaaa aatttcagcg gtttttattc cttcttgggt tgctttgcgc
     1801 gttccaacta tggcgatagt cggttcgttg atcgataagt tgcctttgat ataaagtttt
     1861 tgcggcgggt ctgaaatctc ctttaaaagc ggtggataaa aggggctatc tattgttatc
     1921 gtttttatat cttctttcaa ttgatatatt tataaattgt ttatacttga agcatattat
     1981 aaaatgtcta tatggttaat ttcagaaacc gttttatatt acaggtgttt gcgactttta
     2041 taatcctcat tgttttgatt tcaatcatgg tttatctagg aatccagata aaagaaagcg
     2101 catccactat ttattctaca agacaggacc ttagagacaa aacgcacaac ataagtgagt
     2161 tatcacaatt aagattggct gctaaagaag ccgagccttt tttagagaag cttgaggcgg
     2221 caattccttc taaggatgag ttgttctctt tcgcagatga tttaaaagag attgcgcgca
     2281 tgaacgatct gggtcttaat tttaagtttg ttggagaagc tattataagc ggcggattgg
     2341 gttctcaaaa atttgaggca aatgctcaag gagattatag agacatcacc ttttttataa
     2401 gagaattgga gaggtctggt tatataacca acgttttgga tgttaatata ttaaaacaag
     2461 ggagtttatt caacgcgctc cttattggag aggtttattt caatgctcaa gcgtaaattt
     2521 cataaaatag aattgattct tttagtctca gccaccctaa tatttatagc gttggtttct
     2581 ggcattattt atgctcttgg ctttataagc gggaacgtta ttcatgcttt gtctggcgaa
     2641 ttaggctctc catcgtctgt tagttttaat ctggatagat acaatgagct agatttataa
     2701 taactggatg tccatatcgt ttactcttgc ctaagttgtt tcgcgaatgt aatatgttaa
     2761 atactttagc gcgcgtagtt cagtggtaga acgctgtcct gataagacag aggtcgaagg
     2821 tccgattcct tccgcgcgca cacttcgaca agctcagtgt aaacataata gcttgggtgg
     2881 ggtaggttga tggcatggtt atggtgagag tagtcgaacc attccttccg cgcgcacaga
     2941 tatgggcggt tagctcagtg gtagagtatt tgcatgacac gcaaagggtc acaggttcga
     3001 tccctgtacc gcccaccagc atccggtctt tctaattgac tttaatataa ttctgtgtca
     3061 atctatttta tagtctcagg caagctgaga taagcacctt gataggaggg attctaaata
     3121 gctgaagcgc gagaagaaaa agtgaaagag ctcatcgaga gtcctcacgt agatagggat
     3181 agtttgaaag ctctcttcgc ggttagctcc gctctcgaga gaatgatccc gcgtcttccc
     3241 tctgccgaac agcatcgcag gggtggatat ttttgtttga tgccgaggga aaggagaggg
     3301 cattcagtcg ggacgccaat cttggttatt caagttggcg aggtggacga tccagagaag
     3361 aggcataagt attacaagtt tgccgtagaa aaggcagggc gcctacatgc ttatgctgat
     3421 acagattccc acaaaagtag ctggcagagc aggcatccat cgactgatcg gtggggagga
     3481 gctatcttaa ccaataagtg gattttttcc ttctccggct tgcccgagct tgcagacgaa
     3541 gcaatcatgc tttttgctgc gattacactc gggtttatgg agcgagcgga cgcggatgcg
     3601 atagctcgca taagcgacaa ccagctctac ttcaaattgt tcgtacagag gggctggtct
     3661 gaccacaatc agaccgtctg ggatccagcg cgtatgacct aggcagaacc ccaatatgac
     3721 caactcgatt tttagggttg gtcacttttt tatttaggca tcttgctaca ttattaatat
     3781 atatgttgga gccaaagatc atatatgaaa acgagaacgt tcttgctatt aataaaccgg
     3841 ccggcttgat gatccatgga gttggtggta agcattttcg tgaagggact caaagagagg
     3901 aagaaacttt agttgattgg ataaaagaaa acaggccgga aatagttggc gtgggtgata
     3961 aagatttaga cggcaatctg cctcagaacg accgcccagg catagtgcac cgtttggata
     4021 ggtatacttc tggcgtgatg cttattgcaa agaccaatga atattttcaa tatctgaaat
     4081 ctctttttca aagtcatcag ataaaaaaaa cttatctggc gcttgtcgtg ggttggccag
     4141 acgaagatga gggaattata aataaaccaa tcggtattaa agacgggtca gttaaaagaa
     4201 ccacctttat aaagaaagct aagatggtgc gagaggcctt aaccgaatat aaagtgatta
     4261 gaaaattttt attcatagaa gatgacgttg aacataaata tgctttagtc gagcttaggc
     4321 cacagactgg tcgaacgcac caaattagag ttcatttagc atcaatcggc catcccgttg
     4381 ttggcgatga gctttatggc aaaaaggatg agagattcag tttaaataga tatcttcttc
     4441 acgcagattc aattgaattt cctaaagaag aaggagatag agtaagattc tcagctgata
     4501 tatccgacga atttaaagag gttttaggga aactaaccct gtctaattta ctttaataga
     4561 ggtctttatt aggattgaca ggttcgatat atactaataa tatagcactt acaatgatta
     4621 gcgaagatat actaaataaa ataagaccag gggttaaaat aagagtttca gagagaattc
     4681 ccgcttatat cacctcggca ggcggaaagg gcaaaaagaa agatgataaa gcggcatcta
     4741 agggcggaga gagaatcagt tcttttgagg gcctggtttt agctaggaaa catggcggtg
     4801 agatcggggc tacatttaca gtaagggcta cggttgccgg agtgggtatg gaaaaaatat
     4861 accctataaa ttcaccctta ttaaataagg ttgagatatt gagccagcct aagaaaactc
     4921 acagggcgaa actttattat ttaagaaata tttcagccaa gaagactcgt aagaaaatag
     4981 gcgcagagaa ataatataaa ggtctctgac cccgtcttaa taaattgtct cattctcaaa
     5041 cgaatagact atttatctga tttggaattt gtctcagaaa aaaggtatat tgaaaaactg
     5101 atatgcgcgg gtggcgaaac ggcagacgca ctaccttgag gtggtagcgc c
//
LOCUS       DATDNZ010000054         9595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2243714, whole genome shotgun sequence.
ACCESSION   DATDNZ010000054 DATDNZ010000000
VERSION     DATDNZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243714"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VI432_02135"
     CDS             complement(<1..300)
                     /locus_tag="VI432_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496923.1"
                     /translation="MGYDVTTTGAALLLIGSILPDLVNIPMGIVLIRKYGLDPFRKWS
                     WSGYRHKMPLLYAIYMMVHSLWLPVWIGIIEYLVSGSVPIFALGWTIHSVVDFATH"
     gene            complement(400..951)
                     /locus_tag="VI432_02140"
     CDS             complement(400..951)
                     /locus_tag="VI432_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY4496924.1"
                     /translation="MNNFKFFRTAVRDYRVGALTTSSKYVIRKIIKELADRKFIIEYG
                     AGDGVITKELLKHLPLDGRVVAIELNKDLFNELKKIKDKRLTIINGDIIEISKDLAQF
                     NLPQVDAVISGIPFTFLPSEMRRTVVQNTYDGLSNRGLFIVYQYSLLMKKWLSGVFDK
                     FKSYFEPRNFPPYFIMVGEKKMN"
     gene            1075..1662
                     /locus_tag="VI432_02145"
     CDS             1075..1662
                     /locus_tag="VI432_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496925.1"
                     /translation="MKNKLTITSLLVVALFSGALLSANFTALADEDSNWTNNSSMIKE
                     SDFRKIDKKLSEAFEDVRGLSLKNMPHETLTIIGIGANSTEGRIRMTEANLDAISGTD
                     LEVSVWGMKFKIDASRAKITGPGRSITISDMKVADKLLINGQIDKGTNLVIAEVIFDR
                     SLQRQAVTDIEARINALLEQIKKLQEEFKKSQQGN"
     gene            complement(1695..2177)
                     /locus_tag="VI432_02150"
     CDS             complement(1695..2177)
                     /locus_tag="VI432_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4496926.1"
                     /translation="MECGFIIIQNMELTSRDFKNKEFIPSRFTCDGENINPSLQISGV
                     TKGANSLALIVDDPDAPGSLSTGGWVHWVLWNINPDTVEIKIHGVPPGAIEGVTSFGR
                     SGYGGPCPPSGTHRYFFKLYALNAILDLPESADKKMLEARMNSHIIETAELIGLYKRE
                     "
     gene            complement(2588..3238)
                     /locus_tag="VI432_02155"
     CDS             complement(2588..3238)
                     /locus_tag="VI432_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4496927.1"
                     /translation="MIIKKFLHSCLLLEKDGKKLLIDPGSFSFVEGKIKPEDIGPVDI
                     ILITHQHTDHYFPEALKKILSLKEPKIITSDEIGALLTGEGIKYHAIFDGDEIDIDGF
                     NIKAINAAHGLTPTLPPHNFAYLIDGKLLHPGDSMSVSNDIKCDVLALPISAPWARMI
                     DLLEFGAKFNASKIIPIHEKILADYWIPRAYDISRSYLEKKGKEFYPLEIGESLEI"
     gene            complement(3235..4380)
                     /locus_tag="VI432_02160"
     CDS             complement(3235..4380)
                     /locus_tag="VI432_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4496928.1"
                     /translation="MINKFILFSALSIFLLIPIYPAEAITLEELQIQIQQLINEINKL
                     RGAQPLAVIEFNRNLYYGLENDGDVVRLQDTLIKKGFLKEGLNTGNYFGLTLDAVRMY
                     QRFNGILPTGYFGPITREKLNSEIKLQNDIAFLELRVEELRAIEKQNPKPFEESAPST
                     PSEYQLIVKPNYDLDFLAMKIHDFINTEREQEGLSILAWDNNVAKNLALPHSEDQARD
                     NIEITDPDLLCHYPLIRHEGFVFGYSLGDRLINRGINYLSAGENIVMFSVTKDLVYSY
                     ESDNPPLDCPQVVSFMPGEGSKEERTALYQSVLNESLAVVRALPDINWVNKNWLNVEE
                     MAAKAVQLWLSSPPHKENLLRSGYNYSGVGIAMVNDYLIITQNFIRR"
     gene            complement(4383..5570)
                     /locus_tag="VI432_02165"
     CDS             complement(4383..5570)
                     /locus_tag="VI432_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496929.1"
                     /translation="MLSSERHHFNFFHNKEIDSLYLTLAIISFAEGLISVFVPIYFFS
                     DLKIPLWRIIFFYFLNAAYFVALAILLLPIIKKLSDKMMMFLGIPFMILYFLGLNYVG
                     DFPILFYILPGLLAMNMFLFNTGYHINFSSAADDGYVGREVGTRYMIGALVQFASPFI
                     GGVIITLFGFDKIFIIGSALLLAAVLPMFFFPVRNIKNLKVAPIFDFIKDKHLRPINL
                     SGAGYAMESVVYRFIWPIFIFLAIGNLEEFGGLISAGLLASAIVTYLVGFLSDAHRRR
                     KILYIASSFMAFIWSFRSILATPFLVAGSHIIGNLSNASMMVAWSSQYYKVARMMESS
                     ALFILSREMIFNITRIVFLPILMLAAYFLPIEIFFTISFGAAALGSLMLIISNKMDYK
                     LLK"
     gene            complement(5603..5794)
                     /locus_tag="VI432_02170"
     CDS             complement(5603..5794)
                     /locus_tag="VI432_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496930.1"
                     /translation="MTSKQYLQISGSLFLIIGSVHFLRIIYGWEASVAGWSVPMWISW
                     LALLLAGYLSFNAFSLSKR"
     gene            complement(5889..6176)
                     /locus_tag="VI432_02175"
     CDS             complement(5889..6176)
                     /locus_tag="VI432_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4496931.1"
                     /translation="MAKKLYVGGLSYSTNEDALRDAFAQAGSVESANVIMDKMTGRSR
                     GFGFVEMANDEDAQKAIDMWNGKELDGRTINVNEAKPMEARPPRRDGMRRM"
     gene            complement(6343..6744)
                     /gene="msrB"
                     /locus_tag="VI432_02180"
     CDS             complement(6343..6744)
                     /gene="msrB"
                     /locus_tag="VI432_02180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY4496932.1"
                     /translation="MEEKDWKEKLTPEEYKVLREGGTEVPYSGEYWKTNEKGMYRCKV
                     CGSQLFSSDTKFESKEPGLKGWPSFDNALPGAVEFKEDKNLGMRRTEVVCAKCGSHLG
                     HVFDTGEESKTGKHYCINSVCLKLERDSRAD"
     gene            complement(6798..7343)
                     /locus_tag="VI432_02185"
     CDS             complement(6798..7343)
                     /locus_tag="VI432_02185"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY4496933.1"
                     /translation="MGLFYTGKGDGGESVVGSKKIDKTGVEIYALGELDELNSLIGIV
                     KNIKINTGFKKILHDVQENLFIVQANLANIMLGGKYKSPQLSFDKVREMEKIIDKLEK
                     KLKPAKAFVISGSSISSAWLDYTRAVSRRVERSVLSFGKYNDLSPEIKAYLNRLSSLF
                     FAMARFESKSGRKKEKHPKYK"
     gene            complement(7765..8136)
                     /locus_tag="VI432_02190"
     CDS             complement(7765..8136)
                     /locus_tag="VI432_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY4496934.1"
                     /translation="MILDKPLFTYDSDCGFCDFWIKRWKNITGDAINYSPYVSAKELF
                     SGISMEELSSAVHLFMPDKKVYKGAEAVFRAFALNGRYTWVLWIYKHIWGARYSSEWF
                     YRWVSHHREFLYRVMRFFRLL"
     gene            complement(8232..8930)
                     /locus_tag="VI432_02195"
     CDS             complement(8232..8930)
                     /locus_tag="VI432_02195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY4496935.1"
                     /translation="MHRFIVDNNLQSGLLVIDDEQLLYQLKDVLRLKSGDEIILADGR
                     GKEALVKISGYKGKGVEVEIAGVSDNKNEPDIIVNLYCSILKRDNFELIVQKVTEIGV
                     KNIIPIISKRTVKLDFKRERLEKIIKEAAEQSGRGIIPKLAEPIKFSDAVSLASKETN
                     ILLDISGEPSAFKPTKDSRALSCWIGPEGGWDEEEVNFAKENGFRILSLGLLTLRAET
                     AAIVASYLSVHKMF"
     gene            complement(8938..>9595)
                     /locus_tag="VI432_02200"
     CDS             complement(8938..>9595)
                     /locus_tag="VI432_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4496936.1"
                     /translation="LACGQGFFSRAFYEEGAEVIGVDISEELIGLARKNSPKEIKYYI
                     SDAHKLDFIEKESIDKAVIVLAIQNIENPREVFEECNRVLEKEGRLFIVMNHPAFRIP
                     KESGWGWDPDKELEKGMKGVQYRRIDRYLSELKSKIQMHPSSLRGGSGRANLSDYTIS
                     FHKPLQFYFKALKKSGFAVIGLEEWVSDKKSEPGPRTIAENRARSEFPLFLMLEMRKI
                     "
BASE COUNT         3069 a         2129 c         1542 g         2855 t
ORIGIN      
        1 atgagttgca aaatccacta ctgaatgaat tgtccaaccc aatgcaaaaa taggcacaga
       61 tccgctcacc aaatactcta tgatgccgat ccaaaccggc aaccaaagag agtgaaccat
      121 catataaatt gcatatagca agggcatttt gtgccgatag ccagaccaac tccactttcg
      181 aaatggatcc aagccatact tacgaatcaa gacgatcccc attgggatat tcaccagatc
      241 aggcagaatc gaaccaatta gtaaaagagc tgctcccgtg gtagtgacat cgtaacccat
      301 gaccgcaagg caaaagatta caagcactcc agtcaacaca tgagatattc cgtccaggtt
      361 ttcacctcac caaattaata acattaatta actttattgt caattcatct tcttctcccc
      421 caccataata aaatacggcg ggaaattgcg aggctcgaaa tatgatttaa atttatcaaa
      481 aacccctgag agccactttt tcatcaataa gctatattga taaacaatga ataatccacg
      541 gtttgataat ccatcataag tattttgaac aacagttctt ctcatttccg aaggcaaaaa
      601 agtaaaagga ataccagaga taaccgcgtc cacttgaggc aaattaaact gagcaagatc
      661 tttagaaatt tctataatat caccgtttat aatcgtgagc cttttatctt ttatcttttt
      721 taattcgttg aatagatctt tatttaattc aatagcgact acccttccat ccaacggaag
      781 atgttttagt aattctttag ttataacacc gtctcctgcg ccatactcaa taataaactt
      841 gcggtcggca agttctttaa taatcttcct gataacatat ttggaactag tggttaaggc
      901 gcccacccta tagtcccgaa ctgctgttct aaaaaattta aaattattca ttaaatgtaa
      961 gtttttgtta tttcgctcgt tttatattct aacaagcttt tattatacaa caatacaatt
     1021 aataaacgtc tatccatttt aatcaaaggt cggcgtaaat catttatcaa atacatgaag
     1081 aacaaattaa ccataacatc tctccttgtc gtcgcattgt tttctggagc gttattatcc
     1141 gccaatttca cagccctagc agatgaagat tctaattgga ctaataactc ttctatgatc
     1201 aaggaaagcg atttcaggaa aatagacaag aaactttctg aagcatttga ggatgtaaga
     1261 ggattgtcct taaagaatat gcctcacgaa acccttacga taatcggaat cggagccaac
     1321 tcaacggagg gtaggataag aatgactgaa gctaatttag acgctatctc aggaacagac
     1381 ctagaagtaa gcgtttgggg catgaaattc aaaattgacg cctctagagc aaagattacc
     1441 ggccccggaa gaagcatcac gatatcagac atgaaagtcg cagacaaact tcttatcaat
     1501 ggtcaaatcg acaaaggtac aaatcttgta atcgcagaag ttatttttga tcgctctctt
     1561 caaaggcaag cggtcacaga tatagaagca agaataaatg cgcttttgga gcaaattaaa
     1621 aaactgcagg aagaattcaa aaaaagtcag caaggcaact aattataaaa cccccttttc
     1681 gggggtttta taatttattc tcttttataa agtccaatta attccgcggt ttcaataatg
     1741 tggctattca ttctcgcttc aagcattttt ttatcggcag actccggcaa atcaagaatg
     1801 gcattcaacg cataaagttt aaaaaaatat ctatgcgttc cgctaggcgg gcaggggccg
     1861 ccataacccg acctgccaaa actggttact ccctcaattg cgccgggcgg gacgccatga
     1921 attttaattt ctacagtatc cggattgata ttccatagaa cccaatgaac ccatcctcca
     1981 gtcgacagac ttccgggagc gtcaggatca tcaactatta gagccagact atttgcgccc
     2041 ttagtaactc ctgatatctg cagggatgga tttatatttt ccccatcaca agtaaatctg
     2101 cttggaataa attccttatt tttaaaatct ctgcttgtaa gctccatgtt ttgaataatt
     2161 ataaacccac attccattat tgacaaaata tattaatatg gcattatgtt cctatcagag
     2221 atcgtttggc ggatctccga actcgaaagg tcttaggacg tttcgtgaca cgcacagcta
     2281 agttgcctgg gtcgcctagc ggacccaaag caaaataaac agctgggtta gtcatcaaag
     2341 cagcggcatg tggatctccc ggaacgatga ctataaaaaa tcgggtgcgc gtgaaatgga
     2401 cgcctcgatt catgaagcgc aaaaaggccc tctttttgtc gaggaaccaa cgggttaagt
     2461 gtcgatccgc tggtataata atgacacaag cctgagatgt gtgcggcaag aaacgctcta
     2521 tcctcccacg aaaagagcgc ccttccttcc ccaccgcaca tatctcgggc tctttttctt
     2581 ttttagacta tatttctaaa gactctccaa tctccaacgg ataaaattct ttcccttttt
     2641 tctctaaata actgcgtgaa atatcataag cccgcggaat ccaataatca gccaatatct
     2701 tttcgtggat tggaattatc ttagacgcat taaacttcgc gccgaattct aatagatcaa
     2761 tcattcttgc ccatggagca gatataggaa gcgccaaaac atcgcattta atatcgttgc
     2821 ttacagacat cgaatctcct ggatgcaaca atttaccatc tattaagtaa gcgaaattat
     2881 gtggcggaag cgttggcgtc agcccatgcg cggcattaat agctttaata ttgaatccat
     2941 caatatcaat ctcatctcca tcaaagatcg catgatattt aataccttct ccggttaata
     3001 gagctcctat ctcatcactt gttataattt tcggttcttt taaacttaat attttcttaa
     3061 gcgcctcagg gaaataatga tctgtatgct gatgagtaat aagaattata tcaacaggac
     3121 ctatatcttc aggtttaatt ttcccctcaa cgaaagaaaa actaccagga tctatcaata
     3181 atttcttacc gtctttctct aacagcaaac atgaatgtaa aaacttttta ataatcatct
     3241 tcttataaaa ttctgggtaa taattaaata atcattaacc atcgctatcc ccacgccgct
     3301 ataattataa cctgatcgga gaagattttc tttatgcgga ggagagctta gccataattg
     3361 aaccgctttt gctgccatct cttcaacgtt caaccaattc ttattaaccc aattaatatc
     3421 cggcagagct ctcacgactg caagtgattc atttaaaacg ctttggtata aggcggttcg
     3481 ttcttcttta cttccttcac caggcataaa actaactacc tgagggcaat ccaggggcgg
     3541 attatcagat tcatacgaat agacgagatc ttttgtaaca gaaaacatta caatattttc
     3601 tcccgcagac aaataattta ttcccctatt tatcaatctg tctcccaagc tgtaaccgaa
     3661 tacgaatcct tcatgcctga taagcggata atgacataat aaatctgggt ccgttatctc
     3721 tatattatct ctcgcttgat cttcactgtg tggcagcgct aaattttttg cgacattatt
     3781 atcccacgca agaatactca gcccttcctg ctctctttca gtattgataa aatcatgtat
     3841 cttcatagct aaaaagtcta aatcgtagtt cggtttaaca atcaattgat actcagacgg
     3901 tgtggaaggg gcagactctt caaatggctt aggattctgc ttttcaatcg cgcgcaattc
     3961 ttccaccctc agctctaaaa aggcaatatc attctgaagc tttatttcac tattcaactt
     4021 ctctctagta ataggaccga aatatcctgt cggaagaatt ccgttaaatc tttgatacat
     4081 tctcacggca tcgagtgtca gcccaaaata attacctgtg tttaatcctt ctttcagaaa
     4141 tcctttttta atcaaagtgt cctgaagtct tacaacatca ccatcgttct ctaatccata
     4201 atataaattg cgattaaact ctattactgc tagaggctga gctcctctta atttatttat
     4261 ctcgttgatc aattgctgta tctggatttg aagctcttca agcgttatag cttccgcagg
     4321 ataaatagga ataagcaaaa atatagatag agctgagaat aatataaatt tatttatcat
     4381 gcttatttaa gcaatttata atccatttta ttggaaataa tgagcatcaa gcttccaagc
     4441 gcggctgcgc caaaactaat cgtgaaaaat atctctatgg gtaaaaaata agccgcaagc
     4501 attaaaatag gcaagaaaac gatccttgta atattaaata tcatttctct gcttaatata
     4561 aaaagcgcgg aactctccat cattctcgca actttataat attgactaga ccaagcaacc
     4621 atcatagatg cattagacag atttccaata atatgactgc cagcgaccaa aaacggagtc
     4681 gccaaaatag acctgaaaga ccatataaac gccatgaaag acgacgctat atataaaatc
     4741 ttcctacgcc gatgagcgtc tgacagaaaa ccaacaagat aagtcactat tgcgctcgcc
     4801 agaaggcctg cagatatcaa tcctccgaat tcttctaaat tccctatggc aagaaatata
     4861 aaaatcggcc aaatgaatct ataaacaacc gactccatag cataaccggc tcccgataaa
     4921 ttaatcggac gcaaatgctt gtcttttata aaatcaaata ttggggcgac ttttagattt
     4981 ttaatattcc tgactggaaa gaaaaacatt ggcaaaacgg ccgcaagaag aagtgccgaa
     5041 ccaataataa aaattttatc aaaaccaaaa agagtaataa taactccgcc gataaacggc
     5101 gaagcaaact gcactagcgc tccaatcata tatctagttc caacttctct gcctacatat
     5161 ccatcgtccg cggcgctaga gaaattaata tgatatcccg tattaaaaag aaacatgttc
     5221 attgcaagca gtccgggtaa aatatagaag aggattggaa aatctcccac ataattcaac
     5281 cctaaaaaat ataaaatcat aaatggaata cccagaaaca tcatcatttt atcactaagt
     5341 tttttaataa tcggcagcaa taaaatagcc agggcaacaa aataagccgc atttagaaag
     5401 tagaaaaata ttatccgcca aagaggaatc tttaagtcgg aaaaaaaata tatcgggacg
     5461 aaaacgctaa ttagcccctc ggcaaaagaa ataatagcaa gcgttagata taaagagtct
     5521 atctctttat tatggaaaaa attgaaatga tggcgttcag aagacaacat ctagatgttt
     5581 agaaacctat ttataaatta gcctatctct ttgataagct gaatgcgtta aaagataaat
     5641 aaccggccaa gagtagcgcc aaccaactaa tccacattgg aacgctccat cccgctaccg
     5701 aagcttccca accgtagatt attcttaaaa aatgaacgga tcctattata aggaatagag
     5761 atcccgatat ctgcagatat tgtttgcttg tcatgaatct aaaacaataa cgacctttct
     5821 aaattatttt acactagaat taatgcaata aaaatccgcc ccgttttaag gggcggactt
     5881 tcgatggttt acattcttcg cattccatct cttcgcggtg gtcgggcctc cataggtttg
     5941 gcctcgttga cattaatagt cctgccatcg agttccttcc cgttccacat gtctatagct
     6001 ttctgggcat cttcatcatt tgccatttca acaaagccga agcctcttga tctgccagtc
     6061 atcttatcca tgatgacatt agctgattca acagagccag cctgtgcgaa agcgtcgcgc
     6121 aaagcatcct cgttggtgct gtaagacaga ccgccaacgt ataacttttt agccattgaa
     6181 acttattgtt aagtaggacg actttaattc tctcgccgca agcctatttg gcttgaatac
     6241 tgcccttttt aatagcaaat acttgctcct tatggagctg attacgtctt caagaaactc
     6301 ctacagaaaa gaactataca ataaaggatg taaaaaagca agctaatctg ccctactgtc
     6361 cctttcaagc ttcaaacaaa ctgaatttat acaataatgc ttgccggttt tactctcttc
     6421 tccggtatca aaaacatgac cgaggtggct tccacactta gcgcatacga cttcagttcg
     6481 acgcatacca agatttttat cttctttaaa ttcaactgcg cctggcaaag cattatcaaa
     6541 actcggccaa cctttgagtc caggctcttt agattcaaat ttagtatctg atgaaaacaa
     6601 ttgagatcca caaaccttgc atctatacat gcctttctcg ttcgttttcc aatattctcc
     6661 gctatatggc acctctgttc ctccctcacg aagaacttta tattcttccg gcgtaagttt
     6721 ttctttccaa tctttttctt ccataataat ttatcttgct ttttgctaat gagctattta
     6781 gttgtcttgc tattaaacta tttatacttc ggatgctttt cttttttgcg accactttta
     6841 gattcaaatc gagccatagc aaaaaataaa ctggagagac ggtttagata tgcttttatc
     6901 tccggtgaca agtcattgta tttcccaaaa gatagaacgc tcctctcaac ccgtctagaa
     6961 actgctcttg tataatctaa ccacgcagaa gagatgcttg agcctgaaat cacaaaagcc
     7021 ttagctggtt taagtttctt ttcaagcttg tcaatgattt tctccatctc tctcacttta
     7081 tcaaaactta attgaggcga tttatacttg ccgccaagca taatgtttgc aagatttgcc
     7141 tgaactataa aaagattttc ctgaacatcg tggagtattt ttttaaaacc ggtgtttatt
     7201 tttatatttt taactatgcc gataagactg ttgagctcgt ctaattctcc aagcgcatag
     7261 atttcaactc cggtcttatc tattttttta gacccgacaa cgctctcacc accatctcct
     7321 ttgcctgtat aaaataaacc catacccaca gtatatttta tatactgtgg aatttctaat
     7381 ctataagttc taatttctaa gcaatattta aacataagat tgacaacata aatcataaat
     7441 actaatttaa gcgtagtggt cctaacggac tttaacaatg gagatcaact aatgaccgag
     7501 aaactacaag ttgaaatcat aaccatgcac aacgtcgatt gccctggtca acacaatcct
     7561 aactttatac aaccaagcaa aggcattaat ggtattaata tgtatacatg gtattgtagc
     7621 ttcaatcccc cagcgccaag caaaccctgt ctatttagag caagggtaag tggtatacac
     7681 ggatcaatca ggaatctaat atttgaagcg cagttcaaat agatttataa ccgagttctt
     7741 atgactcggc ttttcttttt atatttacaa taatctgaaa aaacgcatta ctctatataa
     7801 aaactctcga tggtggctga cccatctgta aaaccattct gaagaatatc tcgctcccca
     7861 gatatgctta tatatccata acacccatgt ataacgccca ttaagagcaa aagctctaaa
     7921 gaccgcctcc gcgcctttat aaactttctt atctggcatg aacaaatgaa cggcagagct
     7981 taattcttcc atagaaattc cgctaaataa ttctttcgcc gaaacataag gtgaataatt
     8041 aatcgcatct ccagtaatat tcttccatct ttttatccaa aaatcacaaa aaccgcagtc
     8101 gctatcataa gtgaataatg gcttatcaag aatcattaat aattatttaa agtaaacgat
     8161 ggcggtgaat ataatgatac tgattataag aaggaggaaa aatctaccca ttgataaaat
     8221 tatagcaaaa actaaaacat cttgtgaacg gaaagatatg acgccacaat tgccgctgtt
     8281 tctgcgcgaa gcgtgagcaa acccagactc aaaatgcgga atccattttc cttggcaaaa
     8341 tttacctcct cttcatccca gcccccttct ggaccgatcc aacaagacaa ggccctactg
     8401 tccttcgtgg gtttaaaagc ggaaggctct ccacttatat caagaaggat attagtttct
     8461 ttcgaagcaa gagaaacagc atctgaaaat tttataggct cggcgagttt aggaattatt
     8521 cccctgcccg actgctcggc cgcctcttta attatttttt ccaatcgttc acgcttaaag
     8581 tccagcttta ctgtgcgctt tgaaataata ggtataatat ttttcacccc aatttctgtc
     8641 accttctgaa cgatcaattc aaaattatct cgcttaagta tcgagcaata taaattaacg
     8701 ataatatccg gttcgttttt attatcactc acgcccgcga tctcaacttc cacgccctta
     8761 cctttatatc cggagatttt gacgagggct tctttgcctc taccatcagc aagaataatt
     8821 tcatctcccg atttcaaacg gaggacatct ttaagttgat aaagtaattg ttcgtcatca
     8881 ataacgagta aaccactctg caaattatta tcaactataa atctgtgcaa gcgcatatta
     8941 aatctttctc atctccaaca ttaaaaataa cgggaattcc gatcttgctc tattctccgc
     9001 tatagtcctc ggccccggct cgcttttctt atcagaaacc cactcttcaa gtccaataac
     9061 agcgaatcca gatttcttaa gagctttaaa ataaaactgc aaaggtttat ggaaagaaat
     9121 tgtataatct gatagatttg ctcgccctga accacctcga agactagacg gatgcatctg
     9181 aatcttactt ttcaattccg aaagatatct atcgattcgc ctatattgga ctcctttcat
     9241 tcctttttcc agttctttat caggatccca tccccatccg ctttccttag gaattctaaa
     9301 ggccgggtga ttcatgacaa taaacaacct tccctccttt tctaaaactc tattgcattc
     9361 ttcaaaaact tctctaggat tttctatatt ttgaatagcg aggacaataa ccgccttatc
     9421 tatactttct ttctctataa aatcgagttt atgagcatct gaaatataat attttatttc
     9481 ttttggcgaa ttctttcgag ctagcccaat aagctcctct gatatatcaa ctccgattac
     9541 ttctgccccc tcctcataaa aagctcttga gaaaaatcct tgcccgcaag caaga
//
LOCUS       DATDNZ010000055         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1210780, whole genome shotgun sequence.
ACCESSION   DATDNZ010000055 DATDNZ010000000
VERSION     DATDNZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210780"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="pilM"
                     /locus_tag="VI432_02205"
     CDS             <1..404
                     /gene="pilM"
                     /locus_tag="VI432_02205"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY4496937.1"
                     /translation="AGASLTQSISNGLNINIRRAEDLKKQRGLIGTGGEYELSTLMAP
                     FLDVILGEVKRVKYNYETNYKGKIEKIILSGGGANLKGIEDYVKREFELATEKGNPLS
                     DIEYPQNVAPLINEIGSPFAVSIGLAMKNII"
     gene            405..1070
                     /locus_tag="VI432_02210"
     CDS             405..1070
                     /locus_tag="VI432_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496938.1"
                     /translation="MPTPSQTQNIKETFVQEDLTAGLSWRLFIFLLIIFSTLLFGYWG
                     MEFGYKSFLSNSISNLDEELKVLGAEVQSVDQKNLLAFYSQIVNIRTLLGSHIFSSKV
                     FTLLESLTHDRVAYLSSSVSVQDRTLRLEALTNSYDTAVAQLDTYSRSPSIEKVTLGG
                     IQFVNDVIRFEVDLTLKSEVFDVPPEASPVIQSVELDADASTDSESGSEEEVNINEDN
                     INQ"
     gene            1130..1642
                     /gene="pilO"
                     /locus_tag="VI432_02215"
     CDS             1130..1642
                     /gene="pilO"
                     /locus_tag="VI432_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY4496939.1"
                     /translation="MGSIYIYLSLIRPVYSEVQELRGERSSRIELLNNQDRATSLVNN
                     LLSKYRGLSNVEDALSLILPNKEGVPTLINQLQVIANINNLSFDSSSLQYLALTQGGD
                     AIISPLGTIRITMVLRGTYDNFKKYLSALETNNRLIDLNSLRIEGGGVRGRDALTYNL
                     VVDSYYQAEQ"
     gene            1644..1937
                     /locus_tag="VI432_02220"
     CDS             1644..1937
                     /locus_tag="VI432_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496940.1"
                     /translation="MAIIIQEEKSRFNWFVIVGVGVFFILLSASTYYLFFAETPAIDV
                     FLSPELQRNTRISDIDLNPQAVTDLKEFKILRAYTEPLKPQSSGRSNPFLSIF"
     gene            1941..3680
                     /locus_tag="VI432_02225"
     CDS             1941..3680
                     /locus_tag="VI432_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY4496941.1"
                     /translation="MDTRALIDKLIEKSLLSDELGQKLVLESKETKRSVEEIIYERHL
                     LDEESVADAKSVILGIPYQRVKAELIPEEVIKIIPADTAVNYKVAPLSLDDKLLVVGM
                     VNPEDPRAQEALRFIAKSKNAGLGVYIITPGDLEMVLRRYSPFQSEIDSAIKALNIKP
                     GKGLSQNQRVVSLEEGIVVSEEAPVIKIVTSTLKAAVADNASDIHIEPQKKRLRVRFR
                     IDGALQEVASLPLELHQPIISRIKILSDLKIDESRIPQDGRFRTVIFGRDIDFRVSTF
                     PTPVGEKAAIRVLDPSVGLKGIDSLGIEGHNADLVQEAIKKPYGMILVTGPTGSGKTT
                     TLYALLQILNRDDVNILSLEDPVEYIVAGINQSQVHPEIGYDFASGLRQTLRQDPDVI
                     MVGEIRDAETAELAVHAALTGHIVLSTLHTNNAIGVVPRLIDMDIEPFLLPSSLNLMI
                     GQRLVRQLCNVCKVASDAPQNIQAIIKEELESLNEKDKNSLKFKSPYKIYKAPGCKEC
                     KNKGVTGRIAIFEVLTMTRELEQIISSGATESKLLDESKRQGMVTMRQDGILKALNGL
                     IAIEEVLRETSGI"
     gene            3787..>4453
                     /locus_tag="VI432_02230"
     CDS             3787..>4453
                     /locus_tag="VI432_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY4496942.1"
                     /translation="MDYKEKLNELLLTTARQGASDLHIAVGRKPTLRIDGVLIGLQKE
                     NILTPEDTQGLVLSMLDDEQKTKFLKYGDLDFSYSFEDKARFRVNVFFQRGYMAAALR
                     LIAAEIKTIEELSLPPILHEFTKLSQGFVLVVGPAGHGKSTTLASILDEVNHKSTEHI
                     ITVEDPIEYLFVQDRSIISQREVKSDSPSFHRALRSILRQDPDVIMIGEMRDPESIST
                     AMTS"
BASE COUNT         1386 a          739 c         1010 g         1318 t
ORIGIN      
        1 ttgcgggagc gtctcttacc caatcaatct caaatggatt aaatataaat attagaagag
       61 cggaagacct taaaaaacaa agaggcctta taggaacagg aggagagtat gaattatcca
      121 ctttgatggc gccgttcttg gatgttatac ttggagaggt aaagcgggtt aaatataatt
      181 acgagacaaa ttataaggga aaaattgaga agataatttt gtctggcgga ggagcaaacc
      241 tcaaggggat agaagattat gttaaaagag agtttgagct tgcaactgag aagggcaatc
      301 ctctttcaga catagaatat ccgcagaacg ttgccccgct tataaatgag ataggttcgc
      361 catttgcagt gtcaattggt ctagccatga aaaatattat ttaaatgcca acgccgtcgc
      421 aaacacaaaa tatcaaagag actttcgttc aagaagatct gacggcgggc ctgtcttgga
      481 gattatttat atttctttta atcatttttt caactctttt gttcggctat tggggcatgg
      541 agtttggata taaatctttt ttaagcaatt ccatttcaaa tttagacgag gagctgaagg
      601 ttttaggggc tgaagttcaa tcagtagacc aaaaaaatct gctagctttt tattctcaaa
      661 tagtaaatat aagaacttta ttaggctcgc atatttttag ctcaaaagtc tttacgcttt
      721 tggagtcttt gacgcatgac agggtggcat atctttcctc atctgtatct gttcaagaca
      781 ggacattgag attagaggca ctgaccaatt catacgatac ggcagtagct caactggata
      841 cttatagcag gtctccaagc attgaaaagg tgactttagg tggaattcag tttgttaatg
      901 atgtcataag atttgaagtc gatctcacat tgaagagcga ggtcttcgat gtcccgcctg
      961 aagcgtcgcc ggttattcaa agcgttgagt tagacgcgga cgcatcaacg gatagcgaat
     1021 cgggctcgga ggaggaagtg aatataaacg aagacaatat aaaccaataa taaacatgag
     1081 agcatcaact caaagactat ttattatcct aggaagtttc gccttattta tgggctcaat
     1141 ttatatttac ctgtctttaa tcaggcctgt ttatagcgag gttcaagaat tgagggggga
     1201 gcggtcatcg agaattgaat tattgaataa tcaagataga gcaaccagtt tggtgaataa
     1261 tctcttgagc aaatataggg gactttctaa tgtagaagat gcattgtctc tgattttacc
     1321 gaacaaagag ggcgtaccga ctttgattaa ccaattacaa gtgattgcta atataaacaa
     1381 tttaagcttt gattcttcgt ctttgcaata tctcgccttg acgcagggag gagacgcaat
     1441 tatatcgccc cttggcacta taaggataac gatggttttg aggggtacat atgataattt
     1501 taaaaaatat ctttccgcac ttgagaccaa caatagactt atagatttga attctttgag
     1561 aatagagggc ggaggagtga ggggcaggga cgctctcaca tataatttag tagtcgactc
     1621 ttattatcaa gctgaacaat aatatggcaa taatcattca agaagaaaaa tccagattta
     1681 attggtttgt aatcgttggg gttggcgtat ttttcattct cttgtcagca tctacttact
     1741 atttgttttt tgctgaaact cctgctattg atgtcttctt gagtccggag ttgcagagga
     1801 atacaagaat atctgacatt gatttaaacc cgcaggctgt tacagacttg aaagagttta
     1861 aaatattgag ggcatataca gaaccgttaa aaccgcaatc atcaggacga tctaatccat
     1921 ttctttctat tttttaataa atggacacac gtgctttaat agataaatta attgaaaaaa
     1981 gcttgctttc tgacgagttg ggacaaaaac tcgttctgga gtctaaggag acaaaaaggt
     2041 ccgttgagga gataatttat gagaggcatc ttttagatga agaatctgtt gcagatgcta
     2101 agagcgttat tttggggatt ccgtatcaga gggtgaaagc cgaattgatt ccagaggaag
     2161 ttattaagat tattccggcc gacaccgcgg ttaattataa agtggctccg ttgagtttgg
     2221 atgataaatt gcttgttgtc ggtatggtta atcccgaaga tccaagagcg caagaggcgc
     2281 ttcgttttat agctaagtct aaaaatgcag gactgggggt ttatattatt actcctggcg
     2341 acttagagat ggtattaagg cgttattctc cttttcagtc tgaaatcgat tcggcaatca
     2401 aagctttaaa tatcaagccg ggtaaaggtc ttagccagaa tcaaagggtg gtgtctttgg
     2461 aagagggtat cgtagtgtct gaagaggctc cagtcatcaa aatagttaca tcaaccctaa
     2521 aagctgcggt tgccgacaat gcttcggata ttcatattga gcctcaaaag aaaagattga
     2581 gagtcaggtt caggatagat ggagccttgc aagaggttgc ctctttgccc cttgaacttc
     2641 atcagccgat tatttcaagg attaaaatat tatctgattt aaaaattgat gaatcgcgta
     2701 ttccgcaaga cggaagattc aggactgtta ttttcgggag ggacattgat tttagggttt
     2761 ctacttttcc aactccggtt ggagagaaag ctgcaattag agtactcgac ccgagtgttg
     2821 gattaaaagg catcgatagc cttggtattg aaggtcataa tgctgatcta gttcaagaag
     2881 ccataaagaa accctatgga atgattttgg ttactggccc gacaggaagt ggaaaaacaa
     2941 caactctata tgctctgctt caaattttga atagggacga cgtcaatatt ttgagtcttg
     3001 aagatccggt tgagtatatt gtcgctggca taaatcaatc tcaagttcat ccagagatcg
     3061 gatatgattt tgcttctggt ctaaggcaaa cattgaggca agatcccgat gtgattatgg
     3121 ttggcgaaat cagagacgca gagacggcag aacttgctgt gcatgcggcg ctgaccggcc
     3181 atattgtttt atcgacactc cacacaaata acgcgatcgg cgtcgtgcct agacttattg
     3241 atatggatat tgagccgttt ttacttcctt cttctttgaa tctgatgata ggtcagaggt
     3301 tggttagaca attgtgtaat gtttgcaagg ttgcttctga cgctccacag aatattcaag
     3361 cgattataaa agaagaactt gaaagtctta atgaaaagga taaaaattca cttaaattta
     3421 agtctcctta taaaatttat aaagctccag gatgtaagga gtgtaagaat aaaggtgtca
     3481 ccggccgtat agctattttt gaagttttaa ccatgacaag agagttggaa caaattattt
     3541 cttctggagc tacagagagc aagttattgg atgagtcaaa aaggcagggg atggttacta
     3601 tgaggcaaga cggtattttg aaagcgttaa acggcttgat cgcaatagag gaagttttaa
     3661 gagaaacatc tggaatttaa gaaacggcac agtgtttagt attaggtata agataatgga
     3721 ggcaacgaat atacactcat aatgcaaaat actatatact taatactata tacttaatat
     3781 ttttatatgg actataaaga gaagcttaac gaattacttt taacaacagc aaggcaaggc
     3841 gcctctgatt tgcatatcgc ggttggtagg aagccaactc ttcgaatcga cggcgtgctt
     3901 atcggtttgc agaaagaaaa tattttaacc cccgaagata cgcaaggatt ggttctttca
     3961 atgcttgacg atgaacagaa gacaaagttt cttaaatatg gagatttaga cttttcgtat
     4021 tcatttgaag ataaagcgag gtttagagtc aatgtttttt ttcaaagagg atatatggct
     4081 gcggcgttgc gtttgattgc tgcggaaatt aaaaccattg aagagttgag tttgcctcct
     4141 attcttcatg aatttacaaa actaagtcag ggattcgttt tggttgtggg tccagctgga
     4201 cacggcaagt caacgacgct cgcctctatc ttagatgagg tcaatcacaa atctacagaa
     4261 catattatta cggtagaaga tccgatcgaa tatctttttg ttcaagacag gtcaataatc
     4321 tctcagagag aggttaaaag tgacagtccg agttttcatc gcgcgttacg atctatatta
     4381 agacaggatc cggacgttat catgattgga gagatgagag atccggaatc aatttcaacc
     4441 gcaatgacgt cag
//
LOCUS       DATDNZ010000056         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3541008, whole genome shotgun sequence.
ACCESSION   DATDNZ010000056 DATDNZ010000000
VERSION     DATDNZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541008"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1469
                     /gene="metG"
                     /locus_tag="VI432_02235"
     CDS             33..1469
                     /gene="metG"
                     /locus_tag="VI432_02235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY4496943.1"
                     /translation="MSSKGPLYITTTLPYVNADPHIGFALEIVQADIIARHNSLLGRD
                     VYFNTGTDEHGQKIYDRALKEGKDPQAYVDEYATKFKSLKERLNLYADIHFIRTTDQH
                     HIEAAQELWRRCLKNGDIYKKKYRGLYCVGDEAFMRESDLVDGKCPNHPSMKITEIEE
                     ENYFFSLSKYQEKLIKYLSKKEVIIPEWRRKEAIDFVQRGLEDFSISRVKEKMPWGLP
                     VPNDDSQVMYVWFDALTNYISTLGWPDDKEGNFDRFWNKGFSIQMAGKDQVRFQSIMW
                     QAMLMSADVKTTDEIFYHGFITSGGQKMSKSLGNVIDPIAIAEEYGVDALRYYLARHI
                     HPVEDSDFTMERFKEAYNADLANGLGNLVSRVFKMAASYDVELPQFDNNYSNNEFLDN
                     FEIQKAADAIWSWIRELDGFIEAEQPFKKIKIDSDGAKRDIQQLLKGVYNVSVALKPF
                     LPETTEKILEALKAGKIKEIGALFPRVE"
     gene            1491..1985
                     /locus_tag="VI432_02240"
     CDS             1491..1985
                     /locus_tag="VI432_02240"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY4496944.1"
                     /translation="MNNFNEKDQTDIANFILNNLPDHKQFALGTVDQSGHPWVVCLNL
                     AYDNKINIIWKSSKDTEHSKHIRNNPNVSICIFSETKEKGDFGLYLKGVAHEVVDIFE
                     LETSLKVRYGQKGKEIPPMSEFLGDSSNRIYCAEIQEAWATDSRHIKPKIDLEILRSA
                     VENI"
BASE COUNT          692 a          329 c          472 g          586 t
ORIGIN      
        1 gatagagacg gaggaatgtt ataattgttt tcatgtcatc aaagggcccc ctgtatatca
       61 caaccacgct tccttatgta aacgcagatc cgcatatagg ttttgctctg gaaattgttc
      121 aggctgacat cattgcgcgt cataacagtc tgcttggaag agacgtatat tttaatactg
      181 gcacagacga gcatggtcag aaaatttatg atagagcgct aaaagagggg aaagatccgc
      241 aagcttacgt tgatgagtat gccactaaat ttaaaagtct caaagagaga ttaaatttat
      301 atgccgatat ccattttata agaacaacgg accagcatca tatagaagcc gcgcaagagc
      361 tatggagaag gtgtcttaag aacggggata tctataagaa gaaatataga ggactttatt
      421 gtgtcggtga cgaagctttt atgcgcgaga gtgatctggt cgatggaaag tgtccgaatc
      481 acccttctat gaaaataaca gagattgaag aggagaatta tttttttagt ttgagtaaat
      541 atcaagaaaa acttattaaa tatttatcga aaaaagaggt tatcattcct gagtggagga
      601 ggaaagaggc tattgatttt gtgcaaagag gattggagga tttcagcatc tctcgcgtaa
      661 aagaaaaaat gccgtggggt ttacctgtgc cgaacgatga ttcgcaggta atgtatgtct
      721 ggttcgacgc cttaacaaat tatatttcta ctcttggttg gccagacgac aaagagggaa
      781 acttcgatag attttggaat aaaggttttt caattcagat ggccggcaaa gatcaggtga
      841 gatttcagtc gataatgtgg caggcgatgc ttatgtcggc agatgtaaaa actactgatg
      901 agatctttta tcacggtttt attacgagcg gcggacaaaa gatgtctaag agtcttggaa
      961 atgtgattga tccgattgcg attgcagagg aatatggcgt tgatgctctg cgctactatc
     1021 ttgcgcgcca catccatcca gttgaggact ctgattttac aatggaaagg tttaaagaag
     1081 cttataatgc agatctcgca aacggtttgg gtaatttagt gagtagagtt tttaaaatgg
     1141 cggcgagtta cgatgttgag cttcctcaat ttgataataa ttattcaaat aatgaatttt
     1201 tagacaattt cgaaattcag aaagccgccg atgctatttg gagttggatt cgcgaattag
     1261 atggttttat agaagccgaa cagccgttca aaaagataaa aatagattcc gatggcgcga
     1321 aacgcgatat acagcaatta ttgaaaggtg tgtataatgt gtcggttgct cttaagccgt
     1381 ttttgccgga gacaacagaa aaaatattgg aggctctcaa agccggtaaa atcaaagaaa
     1441 tcggcgccct ttttccaaga gttgaataaa tataatgtag ttaatttgta atgaataact
     1501 ttaacgagaa agatcaaacc gatatagcaa attttattct gaataatctg ccagatcata
     1561 agcagttcgc cttgggaacg gttgatcaat ctggtcaccc atgggttgtt tgtcttaatc
     1621 tagcttatga caacaaaata aatatcatct ggaaatcaag taaagataca gagcattcta
     1681 aacacatacg taataaccca aatgtttcta tttgtatttt cagcgagaca aaagaaaaag
     1741 gagacttcgg cttatatcta aaaggagtcg cgcatgaagt cgttgatatc ttcgaactag
     1801 aaaccagtct taaggttaga tatggacaaa aaggcaaaga aatcccgcct atgtctgaat
     1861 ttttagggga ttcatcaaac aggatttatt gcgcagaaat acaggaggcg tgggctactg
     1921 atagccgcca tatcaaacct aagatcgatt tggaaatatt gcgaagcgcg gtagagaata
     1981 tataaaaaat gaactctaaa agtttaatca atgtaaagaa atataaatta gtcgtatatg
     2041 ttccgcttac tcattcggat atggttcgag aagcaatag
//
LOCUS       DATDNZ010000057         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2506134, whole genome shotgun sequence.
ACCESSION   DATDNZ010000057 DATDNZ010000000
VERSION     DATDNZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506134"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VI432_02245"
     CDS             complement(<1..474)
                     /locus_tag="VI432_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY4496945.1"
                     /translation="MTKFYKISLFVLRISTGWLMFYAGITKVLNPAWSAEGYLSGAKT
                     FSAFYAWFSQPSILPIINFLNEWGLTFLGISLILGVFVRLSSIGGIALMLLYYFPVLE
                     FPYIPGAHAFIIDEHIIYAVVILFLAASKSGRFWGLENWCSNLPICAKYPKFREWL"
     gene            complement(480..926)
                     /locus_tag="VI432_02250"
     CDS             complement(480..926)
                     /locus_tag="VI432_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496946.1"
                     /translation="MNKKFVSLVILGIIFSAGLTVLAHGPMSFSDTSDTRYGMMEYME
                     EQVLGSDIHEEMEELMSRMFVNSMTETDTMRLTSLMNQYPGAGSMMMTRIGGGDVNSF
                     WSPHMSGSWSGWFGFWSILVLSGFIVWWLVGLFLAIWLWRQIKSKK"
     gene            complement(1001..1414)
                     /locus_tag="VI432_02255"
     CDS             complement(1001..1414)
                     /locus_tag="VI432_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496947.1"
                     /translation="MVKRVKKSFWFMTIPIWPVIRMLQGRWRIQWREKDKHIKHIGWF
                     RHDKSLWEFKRHLRRHGFRRFFMAYKDVDELFSMRKIHEERFQYHIRFYRDGRITGHY
                     ELMPELHTIQHLREVGLEPRREEFLEVMGEWIMQE"
     gene            complement(1465..2028)
                     /locus_tag="VI432_02260"
     CDS             complement(1465..2028)
                     /locus_tag="VI432_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4496948.1"
                     /translation="MLYFIDRNYMVFAVEPVKKENNLQEDIDALKPGFDLIDDHVVIT
                     DIDGNIVYANYAVQRNTGFNPGEIVGKNPGILWGGNMPKDFYEKMWHRIKTEKKPFIG
                     EVKNKKKDGAEYWQELRIYPVFDQRGDIKLFIGIEPNITARKSAEDETKKKYDEIDKF
                     NKMMMSKENMQIVELQKRLEDLKSRLT"
     gene            complement(2045..3289)
                     /locus_tag="VI432_02265"
     CDS             complement(2045..3289)
                     /locus_tag="VI432_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4496949.1"
                     /translation="MRFTDLFKIASSGLSRSRMRSALTMLGIVIGISSVILLMSLGAS
                     AERLILDQIRSIGSDLIFVIPGAISGDGFSSPPSAQGVVIKTLVERDVDRLLREPSIE
                     NVAPEVRGQARVVFENEDRTVTFQGTTEAFFKIRDFEIVRGTHFADNDDRSLNRVAIL
                     GSEISEALFDERDPIGKTIRLKDISFKVIGVAGSEGVGPLGVDQDNIILIPMSIAQKQ
                     LLGIDYYNQLTIKANPAYAIEFVQDRVFKTLLDSHRITDPNKEDFTVRSQEDALSILG
                     DISIVLQLFLIAVALISLIVGGIGIMNIMLVSVIERTKEIGLRKSVGATSGDILQQFL
                     LESVILTMIGGIVGTIFGILINVGLYFIISSFVINTWKFSLPASAIILALGVSSVIGI
                     IFGIFPAYKASKKSPIDALRYE"
     gene            complement(3286..>3554)
                     /locus_tag="VI432_02270"
     CDS             complement(3286..>3554)
                     /locus_tag="VI432_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEY4496950.1"
                     /translation="RIAIARALVNDPAIIFADEPTGNLDSKSGAQIMEIFKRLNDDGR
                     TVILVTHETYTANYAGRVIHIKDGMIEKDSVQKNKHSTKNNYFK"
BASE COUNT         1111 a          859 c          517 g         1067 t
ORIGIN      
        1 gagccattct ctaaacttgg ggtattttgc gcatataggc aaatttgagc accaattctc
       61 caacccccag aatctccccg acttcgaagc ggccaagaac aagatcacca ctgcataaat
      121 tatatgctca tcgattataa acgcatgcgc tcctggaata taaggaaatt ccagaaccgg
      181 aaaataatat agaagcatta aagcaatccc cccaatagaa cttaatctca caaaaactcc
      241 tagtatcaga gagataccga gaaaggttaa tccccattca tttaaaaaat taattatcgg
      301 aaggatgctt ggttgcgaaa accacgcata aaaagctgaa aaggttttgg ctccactaag
      361 atatccttct gcagaccacg ctggatttag aactttggtt attccggcat agaacatcaa
      421 ccatccggtt gaaatgcgta aaacaaacaa tgatatctta taaaattttg tcataatatt
      481 tactttttag acttaatttg tcgccaaagc caaatcgcga gaaataaacc gaccaaccac
      541 caaacaataa atcctgatag cactaatata ctccagaagc cgaaccatcc agaccaagac
      601 ccagacatat gcggactcca gaagctatta acgtcgcctc cgcctattct tgtcatcatc
      661 atgctcccag cgccgggata ctgattcatt aaagatgtga ggcgcatcgt gtccgtttcc
      721 gtcatagaat taacaaacat cctggacatc aattcttcca tttcctcatg gatatctgat
      781 cccaaaacct gctcttccat atattccatc atgccatatc tagtgtccga agtgtcgcta
      841 aaactcatcg gcccatgcgc caacaccgtt aatccggcgg agaaaataat tcctaaaata
      901 actaatgata caaatttttt attcataata ttctttatag ataattttat ccgaccttta
      961 ttttcgatag tctgatttat taaataaact atcaaagcga ctactcttgc ataatccact
     1021 cccccatgac ctccaagaac tcttccctcc tcggttctaa acccacttca cgcaagtgtt
     1081 gtatggtgtg aagctccggc ataagctcgt agtgtccggt aattcttccg tctcgataaa
     1141 atcttatatg atattgaaat ctttcttcgt gaatttttct catgctaaaa agctcatcta
     1201 catctttata ggccatgaaa aacctcctaa aaccatgtct tcttaaatgt cttttaaatt
     1261 cccatagact cttatcatgc ctgaaccaac cgatatgttt gatatgctta tctttttctc
     1321 tccactgtat tctccatctg ccctgcaaca ttctaataac aggccatatt ggaatggtca
     1381 taaaccagaa agatttttta actcttttaa ccatcgtttt attttatagc ttacgcgttt
     1441 tattagaatt aactatccaa aaaattaagt caatctactc tttaaatcct caagcctctt
     1501 ctgaagttca actatctgca tattctcttt gctcatcatc atcttattaa atttatctat
     1561 ctcatcatat tttttcttag tctcatcttc ggcgcttttt ctggcggtta tattcggctc
     1621 tatgccaata aataatttaa tatccccgcg ttgatcgaaa accggatata tccttaattc
     1681 ctgccaatat tcagctccat cttttttctt attcttaacc tctcctatga acggcttttt
     1741 ctctgttttt attctatgcc acatcttttc ataaaaatcc tttggcatat taccacccca
     1801 taagatacct ggatttttac cgacaatctc accaggattg aaaccggtat tcctctggac
     1861 tgcataatta gcataaacaa tattgccatc aatatcagtt ataacgacat ggtcatcaat
     1921 taaatcaaaa cccggcttta gagcgtctat atcttcttgc aaattattct cttttttaac
     1981 tggctctacc gcaaaaacca tataattcct atcaatgaag tatagcacca cttaaaaata
     2041 tgggctactc atacctcaag gcgtctatcg gacttttttt cgaagcctta tacgcaggga
     2101 atataccaaa aataataccg attactgaag agacccctaa agctaagatt atcgccgagg
     2161 caggcaaaga aaatttccac gtattgataa caaaactaga tatgatgaaa tataatccca
     2221 cattgataag aatcccaaag atcgtgccta cgatcccgcc tatcatggtc aaaatcacag
     2281 actctaacaa aaattgctga aggatatccc cacttgtcgc tccaaccgat ttacgaagtc
     2341 cgatctcttt agttctctct atcacagaca caagcattat attcataatt ccaatacccc
     2401 ctacgatcaa agaaattaat gctacggcta tcaaaaacag ctgaagcact attgatatgt
     2461 cgcccagaat agataaagcg tcttcttgcg atcttactgt aaaatcttct ttattcggat
     2521 cggtaatacg gtggctatca agaagcgtct taaaaactct atcctgaaca aactctatcg
     2581 catatgctgg attggcctta atggtcaact gattgtaata atcaattccc aagagttgtt
     2641 tctgagcaat tgacatcgga atcaaaataa tattatcctg atcaacgccg agcggcccaa
     2701 ctccctccga accggctacc ccaataacct taaatgatat atcttttaat ctaatcgtct
     2761 ttccaatcgg atcacgctca tcaaaaagag cttcagatat ttcgcttcct aaaatagcaa
     2821 ccctgtttaa agacctatca tcattatccg caaaatgagt tcctctcacg atctcaaaat
     2881 ctcttatctt aaaaaatgcc tcggtggttc cctgaaaagt gactgtacga tcttcgtttt
     2941 caaaaacaac cctcgcctgt ccacgtacct caggagctac attttctata gacggctctc
     3001 taagcagcct gtccacgtct ctttcaacca gggttttgat gacaacacct tgagccgaag
     3061 gaggagatga aaatccatca ccacttatag ctccagggat aacaaatatc aaatctgaac
     3121 caatgcttct tatctgatct aaaataaggc gttcggcaga agctccaaga gacattaaaa
     3181 gaataaccga cgatatgccg ataactatac cgagcatggt aagcgccgat ctcatacgcg
     3241 aacgactcaa tccactggaa gcgattttaa ataaatccgt aaacctcatt taaaataatt
     3301 attctttgtt gagtgtttat tcttttgaac actatctttc tcgatcattc catctttaat
     3361 atgaataacc ctaccagcat agttcgctgt ataagtttca tgagtcacaa gaataacagt
     3421 tcgcccatca tcatttaaac gcttaaatat ctccataatt tgtgcgccgg atttcgagtc
     3481 caaattaccg gtcggctcat cggcaaagat tatagccggg tcgttcacta acgctcgcgc
     3541 aatcgcaatt cttt
//
LOCUS       DATDNZ010000058         3283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1482655, whole genome shotgun sequence.
ACCESSION   DATDNZ010000058 DATDNZ010000000
VERSION     DATDNZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482655"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VI432_02275"
     CDS             complement(<1..661)
                     /locus_tag="VI432_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4496951.1"
                     /translation="MKSRSKKSGFTLVEVMIVLGVITISGAVVTLILNPAEIIRASRD
                     SARLTDIKTLNQAIDLYSHSQNPYFGEDNIIYTSLPASPNTDCSDWNLPPITGWSYKC
                     ATEDNLRRVDGNGWIPINFTESSFGGSPLSVLPIDPVNASDGGLYYTYVKGSWELTAK
                     MESNKFSSGGSHDTETSDGGNNDSRYQLGSRLEITPEELGNGGSPGGYAQSSYYSQSG
                     YT"
     gene            complement(664..1305)
                     /locus_tag="VI432_02280"
     CDS             complement(664..1305)
                     /locus_tag="VI432_02280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4496952.1"
                     /translation="MLKNSLIIILLIIITVTGIYLLTKVGDKENNTNDTQPPMQFLEI
                     DPSKSYTAVIKTTLGDVSIKLNAKTAPITSNNFVSLARKEFYNGTIFHRVINGFMIQG
                     GDPNTKQPESSTNVYGTGNPGYKFDDEAFEGEYLRGTIAMANSGPNTNGSQFFIMHRD
                     YPLPKNYVIFGEVINGLDAVDKIAESNVTISASGELSKPVQPTRIESIEIIES"
     gene            1731..2015
                     /locus_tag="VI432_02285"
     CDS             1731..2015
                     /locus_tag="VI432_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496953.1"
                     /translation="MTRERQSQIENGPGKFDLAAALFKGDPVRFSLAWGGEVQAKITS
                     AATIGQDRDRWLLRGFTGEQPFKAQYNVRTRRGLFDRVKPDELEKAVQKL"
     gene            complement(2139..2636)
                     /locus_tag="VI432_02290"
     CDS             complement(2139..2636)
                     /locus_tag="VI432_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496954.1"
                     /translation="MINRLLILMGIVTTMFTPTVIAHGNELSFEKQVDDFLIDIGYGE
                     SQITAGAPTRLDFNIVDTKKNETVEFDNVFVRINEGEKTIFAGSILKSIFSRGITYTF
                     PHGGDYELYIRFQKDTDAITEAAFPIKVLNNDADPDSTPISYFLISGVLGLIIGFGSS
                     KFFKK"
     gene            complement(2681..>3283)
                     /locus_tag="VI432_02295"
     CDS             complement(2681..>3283)
                     /locus_tag="VI432_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496955.1"
                     /translation="TQNKLIIATDISYEFESGLKIHKKIIEKLARAPITLISTAPRIK
                     NAETSNEISLSIIDKIDESIDVLFVEKIKNRGESAEKERIDLEMKALNTITDEKIKNS
                     VKRDVESVEVKIREMRQIIEKQISVFEQKTQEQLIHRLRITENIFEEGRAKAKDETYK
                     DAFTLLQKAKTIANETKPLIEAKNRFGIEIDFSITNGKNE"
BASE COUNT          843 a          605 c          684 g         1151 t
ORIGIN      
        1 atgtatagcc ggattggcta taataacttg attgcgcata tcctcctgga ctgcctccat
       61 tacctaattc ttcaggcgtt atctctagtc tagatcctaa ttgatatctt gaatcgttgt
      121 tgcctccgtc tgatgtttca gtgtcgtggc tgcctccaga actgaattta ttagattcca
      181 ttttagctgt aagctcccaa gatcctttta cgtaggtgta ataaagtccg ccatcagatg
      241 cattaaccgg atcaatcggt agaacggata ggggagatcc gccgaatgat gattcggtaa
      301 aattgatcgg tatccagccg ttgccgtcta ctcttcttaa attatcttct gtagcgcatt
      361 tataactcca gccggtgatt ggcggtagat tccagtcgga gcagtccgtg tttggagatg
      421 ccggtaatga tgtgtatata atattgtctt ctccaaagta tgggttttgc gaatgactgt
      481 aaagatcaat agcttgattt agagttttaa tatctgtgag cctcgcgctg tctctgcttg
      541 cccgtatgat ctcagctgga tttaatatga gtgttacaac ggctccgctt atcgttataa
      601 cacctaaaac aatcattact tctactagcg tgaagcctga ttttttagat ctcgatttca
      661 agtttaagac tctattattt ctattgattc aattcttgtg ggttgaacgg gttttgataa
      721 ttccccgctc gcgcttattg ttacattgga ctcagctatt ttatcaaccg cgtctaaacc
      781 gtttataact tctccaaaaa ttacataatt tttaggcaga ggataatctc tgtgcataat
      841 gaaaaactgg ctgccgtttg tattgggacc ggagttagcc atcgcaattg ttccgcggag
      901 atactctccc tcaaaggctt catcatcaaa tttatatccg ggattgccgg taccataaac
      961 attagtagag gattccggct gtttggtgtt tggatctcct ccttggatca taaatccgtt
     1021 gatgactcta tgaaatattg tcccgttata gaattctttc cgcgccaaag atacaaaatt
     1081 attagatgtt atgggagcag tcttagcgtt caattttatg gagacgtctc ctagagtcgt
     1141 tttaataacg gctgtataag attttgaagg gtctatttct aaaaattgca taggtggttg
     1201 tgtgtcgttt gtgttgtttt ctttatcgcc gaccttagtt aataaatata tcccagttac
     1261 tgttattata atgagtaaga tgatgattag gctgttttta agcatattat aataatatac
     1321 gattttttgt ataatataag tgcatgctct ttaaactttc gcagatgaat gcgaattata
     1381 cccataaagg atggtagctt gtatggcaag aaacgatgga gctgttttta cgccatggat
     1441 aggccgcaga ttttattacc gtccggcgcc aaggtctgaa cagcgcaaat atccgttacg
     1501 cgcttaaatg gggaagagtt agggggagat tatgccccct tatatttttt tatgtttcat
     1561 attgtaagct ggctgatttt cggatgagat gaattaaagt tataaagata tattttaagc
     1621 ttttgttgag tatttttaat ttaaggcttt tataatttga ttatttgaca gaggaaactc
     1681 attcaattag tttgttaatg agttcttttc cagaaaaaag aagtgtcgtt atgacgcgag
     1741 aacgccaaag tcaaatagag aatgggccgg gtaaatttga tctggccgcc gcgctattca
     1801 agggagatcc ggtgcgtttt tctcttgctt ggggcggaga ggtgcaagca aaaattactt
     1861 ctgcggccac gatcggccaa gatagggaca gatggcttct aaggggtttt actggagagc
     1921 agccgtttaa ggcgcagtat aatgttcgta ctcgccgagg attatttgat cgagtaaaac
     1981 cagacgagct tgagaaggct gtccaaaaac tttagagaaa gtgtccatcg tgcagttttc
     2041 tgatggttag caacggcact tgttataagt gtatcagttg cggtgaaaca gatggatgct
     2101 cctgatggat agacgggtct gcattaaaag tgcggaccct attttttaaa aaattttgaa
     2161 gagccgaaac caataataag acctaaaacc cctgatatta aaaagtatga tatcggtgta
     2221 gagtcagggt cggcgtcgtt atttaaaact ttaattggaa aagctgcttc ggtaatagca
     2281 tctgtgtctt tttggaatct gatatatagc tcgtaatctc ctccatgtgg aaaggtatac
     2341 gttattccgc ggctaaatat actttttaaa attgagcctg cgaaaatagt tttctctcct
     2401 tcatttatcc tgacaaatac attatcgaat tcaacggtct cgttcttttt agtgtcaacg
     2461 atgttaaaat caagtcgtgt cggtgcgccg gctgttatct ggctttctcc gtagccgata
     2521 tctatcaaga agtcgtccac ttgtttctca aaagagagct cgttgccgtg cgcaattacg
     2581 gtcggcgtaa acatagtagt cactatgccc attaatatta aaagacgatt tatcattttg
     2641 ttattataag ctctgtaaat tcgcaaatca atctctaaat ctattcattc tttccgtttg
     2701 taatgctgaa atctatctcg atgccaaatc tatttttagc ttcaataaga ggctttgtct
     2761 cgttggctat cgttttagct ttctgcagaa gggtgaaagc gtctttatag gtttcgtctt
     2821 ttgcttttgc gcgcccttct tcaaagatgt tttccgttat tctgaggcgg tggatcagtt
     2881 gttcttgtgt tttttgttca aacacgctta tctgtttttc tatgatttgt ctcatctctc
     2941 ttatttttac ttctaccgat tccacatctc gtttgactga atttttaatt ttttcgtccg
     3001 ttatggtgtt taaggctttc atctccaggt cgatacgctc tttttcagct gactcacctc
     3061 gattttttat cttctcgaca aataatacgt ctatactttc atcaatttta tcaatgattg
     3121 ataaggatat ttcgtttgag gtctccgcat ttttaatcct aggcgcggtt gatatcaggg
     3181 tgataggagc tcttgcaagt ttctctatta tttttttatg tatttttagt ccggactcga
     3241 actcataact tatgtctgtg gctataatca gtttattttg agt
//
LOCUS       DATDNZ010000059         4864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2516865, whole genome shotgun sequence.
ACCESSION   DATDNZ010000059 DATDNZ010000000
VERSION     DATDNZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516865"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..2388)
                     /gene="recG"
                     /locus_tag="VI432_02300"
     CDS             complement(271..2388)
                     /gene="recG"
                     /locus_tag="VI432_02300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4496956.1"
                     /translation="MLNSPISEIRGVGLKFLPKLKKLGIETAEDLLRHFPTRYEDWST
                     FSSIEKLNPGDEKTISGEVTKINVRRAWRKKTLLVEASIQNGDKTIRAIWFNQPYIKN
                     ILKIGTKANFAGKVVLRDKKIYLQNPAYEVINGGESKHTGRIIPIYSETKGLTSKGIR
                     YLISILLEETEKIEEFMPGDILEKYNLPEINKGINDIHFPKNKKAAEIAQKRFAFEEL
                     FLLQIKNAKHKLALKNNTAPKIIWTNKELQTILEKLPFTLTNSQKESLIEILKDLSSG
                     SPMNRILQGDVGSGKTIVAGLATIITAKQDYQIAIMAPTEILARQHYKTLIKFFADYN
                     IPIGLLLSKETRIFYEDGLEAEPKKESFKNSVREGIIKIVIGTHALIQKGIEFKNLAL
                     VIIDEQHRFGVEQRKKLLANKKGDVVSHFLSMSATPIPRTLMMTIFGDLDVSQISENP
                     KGRKEIITKIVDPINRDKAYAFIRGQVRRGRQVFVVCPRITSRNGETDNKIVDPKERL
                     LWEVKAVEDEHEKLKNRIFPDLKVSMLHGKMKGAEKEKTMRAFSEKKSDILVSTSVIE
                     VGIDVPNATIMMIEGADRFGLAQLYQLRGRVGRGEHQSFCFLFTESHSEQTHARLKSI
                     AEAKNGLELAKIDLALRGPGELLGKDQAGMPDIAMSSLQNPRLIKETRDAAIDLLKHD
                     PEFEKHQKLIKKIEIFEEKIHLE"
     gene            complement(2485..2555)
                     /locus_tag="VI432_02305"
     tRNA            complement(2485..2555)
                     /locus_tag="VI432_02305"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2521..2523),aa:Lys,seq:ctt)
     gene            2681..3235
                     /locus_tag="VI432_02310"
     CDS             2681..3235
                     /locus_tag="VI432_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496957.1"
                     /translation="MHKGPLKLEAKEIMTWDKTSVIVGADLQPPENVEICVQPFSTFK
                     QFKAAAKDADLIITSEAGCHWMTSKKRPYWIDGVGILPYDERMPVVVAAMLKSEGLEA
                     LTCEEGVALVRAIKSGRISVPQDCTSIDLLACGGVRPGGRVVALRPSSKKGWTKMELF
                     TRIGDGRHGHFFAVKRPLPKGAGE"
     gene            complement(3288..3359)
                     /locus_tag="VI432_02315"
     tRNA            complement(3288..3359)
                     /locus_tag="VI432_02315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3324..3326),aa:Arg,seq:tcg)
     gene            complement(3375..4055)
                     /locus_tag="VI432_02320"
     CDS             complement(3375..4055)
                     /locus_tag="VI432_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496958.1"
                     /translation="MAKVYEKREVILLRRRGHTYKEIMKLIPNVAKGTISIWCNSHRF
                     TPDEIKKINDRSQIGRDKARLAAAKTNHALRLERDKIILDEATKLFEKYKNEPLFILG
                     VSLYWGEGTKTQRHFQFTNSDPEMISAMMNWLEKYLNTPREIIRPRLYMHLHKNNTIS
                     EYEHFWSKYLKIPISNFHRTVIKKTNYHFNKKKGYSGCIRIEAAGGVKSWLTVMAWRK
                     LLVENVKI"
     gene            4144..>4864
                     /locus_tag="VI432_02325"
     CDS             4144..>4864
                     /locus_tag="VI432_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496959.1"
                     /translation="MTKDLSPKIPEEVLLVAKTLIKRGHKAYLVGGCLRDLLIKKTPY
                     DWDIATDAIPDEVQKVFKDTIYENSFGTVGVKTDSEDQRLKVIEVTTFRKEGKYTDKR
                     HPDDVKFAETIQEDLARRDFTVNAIALSVDEKKSELIDPFDGMADLDKKIIRAVGISE
                     ERFEEDALRLLRAVRLATQLGLSQGWTIEGETKKAIIKKSHLLEFIAKERIRDEIIKL
                     IMSDDAHDGVRLLEEVGLLKYI"
BASE COUNT         1259 a          942 c         1019 g         1644 t
ORIGIN      
        1 gcctttcggt cgttccgttg ttgtcgatgc ggaaggcgat gcacgggttt accgttgccc
       61 gctttccgta cgcttcctct acggcttgag ccgttcgcgc gtccttcagt tcgatgcctg
      121 gggttttcat gtgttccact tccggtactg tactctaccg cagtattaaa ttgtttaggt
      181 gcggtgttat gaatgcaggc taagtgtcta cattctaact aagatatatt accatataga
      241 ttttaaaaag tcaagtccct gtatataagc ctattctaaa tgtatttttt cttcaaaaat
      301 ttcaattttc tttataagtt tctgatgttt ctcaaattca gggtcgtgtt ttaataagtc
      361 aattgccgca tctctggttt cttttattaa tcttgggttt tgcaaagagc tcatcgctat
      421 atcaggcatg ccagcctgat cttttcccaa gagttcgccc ggacctctta atgctaggtc
      481 tattttggca agctccaacc cgtttttagc ttcggcgatt gatttaaggc gcgcatgggt
      541 ttgttcggag tgagattcgg taaataaaaa acagaaagat tgatgttcgc cccgcccgac
      601 tcgtccgcga agttggtaga gttgggctag gccaaatcta tccgccccct cgatcatcat
      661 gatcgtagcg tttggcacgt ctatgccgac ttctataact gaagtagaaa ccaggatgtc
      721 agactttttt tcactaaaag cccgcattgt tttttctttt tccgcgcctt tcattttccc
      781 atgaagcatg gaaactttaa ggtcggggaa tattctattt tttaatttct cgtgttcatc
      841 ttcaacggct ttcacctccc ataataatcg ttctttcggg tcgactattt tattatctgt
      901 ttctccgttt ctgctagtaa ttcgcggaca gaccacgaag acctgtcttc cgcgtcttac
      961 ttgcccgcga ataaatgcat aagctttgtc tcgatttatc ggatctacaa ttttggtgat
     1021 tatttctttc ctgccctttg gattttcaga tatttgtgaa acatctaaat ctccgaagat
     1081 agtcatcatg agcgttcttg gaatcggcgt cgcgctcatt gagaggaaat gggagacgac
     1141 atcacctttt ttatttgcga gcagcttttt tctttgttca actccgaagc gatgctgttc
     1201 atcgattata acaagggcga gatttttaaa ttcaattcct ttttgtatta atgcatgagt
     1261 gcctataact attttaataa taccttccct aacgctattt ttaaaagatt ccttcttggg
     1321 ttccgcttca agcccgtctt cataaaaaat tctagtttct ttagagagga gcaaaccgat
     1381 tggaatatta taatcggcga aaaacttgat aagggttttg tagtgctgtc tcgcgagtat
     1441 ctcggtgggc gccatgattg cgatttgata atcctgtttg gcggttatta ttgtcgctaa
     1501 tccggcaact attgttttgc cggatcctac atctccctgg aggatcctgt tcattggaga
     1561 tccagacgac agatctttta agatttcaat taaagattct ttttggctgt tggttagagt
     1621 aaagggaagt ttttcgagga ttgtctgtag ttctttgttg gtccaaatta tttttggcgc
     1681 tgtattattt ttaagggcaa gtttatgttt ggcattttta atttgtagta agaataattc
     1741 ctcgaaggcg aacctttttt gcgctatttc cgcagctttt ttatttttcg ggaaatgaat
     1801 atcatttata cctttattta tttcgggtaa attatatttt tctaaaatat cgccgggcat
     1861 aaattcttca attttttctg tttcctcaag cagaatagaa attagatatc ttatgccttt
     1921 ggatgttagg ccttttgtct ccgaatatat tgggattatt cgtccggtat gttttgattc
     1981 tccgccattt attacttcgt atgccggatt ttgcaggtat atttttttat ctcttagtac
     2041 gactttgccg gcaaaattcg cttttgtgcc tatcttcaaa atatttttaa tgtatggctg
     2101 gttgaaccaa attgcgcgga tggttttatc accgttttgt atagatgctt caaccaagag
     2161 ggtctttttt ctccatgctc ttctgacatt tattttcgta acctcgccgc ttatagtttt
     2221 ttcgtctcca gggtttaatt tttcgattga agaaaatgta gaccagtctt cgtatcttgt
     2281 ggggaagtgg cgcaataagt cttctgcagt ttctattccc aactttttta atttgggaag
     2341 aaatttaagg ccgactcccc gtatttcaga aatgggactg tttagcatag tttgtagcat
     2401 ataaatagta tattactaac aattgcctat gagcgactag cggccaacga aaaagtcggt
     2461 caattttctg aaagtcagtt tttgtgcccc cactaggaat cgaacctaga tctgcggatt
     2521 aagagtccgt agctttacca ctaagctata ggggcgtagt tatattaaaa taaatgcttt
     2581 ttttaaagct gtctacttat atagtaatga tctttaagtc aactgtgtgg actttgccca
     2641 tgaattttgc tatagtcaag ggagttatgc aatagcaata ttgcataaag gacctttaaa
     2701 actggaggca aaagagataa tgacctggga taagactagc gtaattgtag gggctgattt
     2761 acagccgccc gagaatgtag aaatctgcgt tcaacctttt tctacattca agcagtttaa
     2821 agcagcggct aaggatgcag accttattat tacgtctgag gctggatgtc actggatgac
     2881 atccaagaaa cgcccttact ggattgatgg agttggaatt cttccttacg atgagcggat
     2941 gccggttgtt gtggctgcta tgctgaagag cgagggattg gaggctctta cttgtgaaga
     3001 gggcgttgcg ctcgtaagag caattaaatc tggaaggatt tctgttccgc aggattgtac
     3061 aagcattgat ttgcttgctt gcggaggggt aagaccaggc gggcgggtcg ttgcgcttcg
     3121 cccatcgagc aagaagggat ggacgaaaat ggaacttttc actcgtattg gtgacggtcg
     3181 ccatggacat ttttttgccg taaaaagacc gcttcctaag ggagcgggcg agtaggcaca
     3241 tttgcgttcc gacgattgtc gtcggaacgc atttatttta gcctcggtgc cctcgccagg
     3301 gatcgaaccc agaactcgaa gttcgaagct tcgcgtgatg tccatttcac cacgagggca
     3361 acatcaccac tctattaaat tttaacgttc tcaacaagta gttttcgcca ggccatcact
     3421 gtaagccagc ttttaactcc gccagcagct tctatgcgta tgcaaccact gtaacctttc
     3481 ttcttattga agtgataatt agttttctta ataaccgtgc gatgaaaatt tgatataggt
     3541 atttttaggt atttagacca aaaatgctca tattcactaa ttgtattgtt tttgtgcaag
     3601 tgcatatata gccgcggtct aattatttcc cttggtgtat ttagatattt ctccagccaa
     3661 ttcatcatcg cgcttatcat ttccggatct gaattcgtaa attgaaagtg tctttgtgtt
     3721 ttcgtccctt ctccccaata gagcgacaca ccgagtataa ataagggttc atttttatat
     3781 ttttcgaaga gcttagtcgc ttcatctaaa ataattttat ctcgttcaag ccttagcgcg
     3841 tgattcgttt tagccgctgc caacctcgcc ttatctctac caatttgtga cctatcatta
     3901 atcttcttta tctcatctgg tgtaaatctg tgactattac accaaataga tattgtcccc
     3961 tttgcaacat tagggataag cttcatgatc tctttataag tgtgccctcg ccgtcttaaa
     4021 agaataacct cgcgtttttc ataaacctta gccatctatt tatatactaa ttataccacg
     4081 ttcaataata aaaaggactt gataatagat atatactgta ttcaaagtta taatccatcc
     4141 gtaatgacca aagatttatc acccaaaatt ccagaagaag ttttattagt tgcaaagact
     4201 ttaattaaac gtgggcacaa ggcttatttg gtgggggggt gcttgcgaga tcttttgata
     4261 aaaaagactc cttatgattg ggatattgcg acggacgcaa ttccagatga agtccaaaaa
     4321 gtttttaaag acactattta tgaaaacagt ttcgggaccg ttggcgtaaa gacagattcg
     4381 gaagatcagc ggctaaaagt gatagaggtt actactttta gaaaagaggg taaatatact
     4441 gataaacgcc atccagatga tgtaaaattt gcagaaacca ttcaagaaga tctggctaga
     4501 agagatttta cggtaaacgc gattgcttta tctgttgacg aaaagaagtc ggagcttatt
     4561 gatcctttcg atggaatggc tgatttggat aaaaaaatta ttcgcgcagt cggaatttcg
     4621 gaagagcgtt ttgaggaaga cgctctccgt cttctgcgcg cggtgcgtct tgcgacccaa
     4681 ttggggcttt ctcaaggttg gactatagag ggcgaaacta aaaaagcaat tataaaaaaa
     4741 tcacatcttt tagagtttat tgctaaagaa agaattcgtg atgagataat aaaacttatt
     4801 atgtctgatg atgcgcacga tggagtcagg ctcttggagg aagttgggct tctaaaatat
     4861 attt
//
LOCUS       DATDNZ010000060         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3561837, whole genome shotgun sequence.
ACCESSION   DATDNZ010000060 DATDNZ010000000
VERSION     DATDNZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561837"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /gene="nusG"
                     /locus_tag="VI432_02330"
     CDS             complement(<1..468)
                     /gene="nusG"
                     /locus_tag="VI432_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY4496960.1"
                     /translation="MFKTLKPEKEKIKHWYAVHTYSGYEDAVARYLKTRVESLSMNDK
                     ILNIIVPKEKKIKIRNGKRITVEEKIYPGYVLVEMIMEPDSWYVVRNTPRVTGFVGAD
                     PTNPAPLSYEEVEDLMVRMGRKEAKFKVDLTVGEMVRITDGPFKDYDGKISEVD"
     gene            complement(470..652)
                     /gene="secE"
                     /locus_tag="VI432_02335"
     CDS             complement(470..652)
                     /gene="secE"
                     /locus_tag="VI432_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY4496961.1"
                     /translation="MITKLKLFLQESRQEFKRINWPTRKETMRMVFIVVTLSVMVSIF
                     LGLWDYIFIYLLELVV"
     gene            complement(683..2641)
                     /gene="gyrB"
                     /locus_tag="VI432_02340"
     CDS             complement(683..2641)
                     /gene="gyrB"
                     /locus_tag="VI432_02340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY4496962.1"
                     /translation="MAKNESKKEEKKTGSAGSGQGGYSAKDIYVLEGLEPVRKRPGMY
                     IGSTGVSGLHHLIWEVVDNSIDEAMGGYAKNIEIRLLKDGVISVSDDGRGIPVDVHKQ
                     TKVSALETVMTTLHAGGKFGGDSYKVSGGLHGVGVSVVNALSEWVKVEVNRDGGIYSQ
                     EYERGAAKNRVKKIGSSEKTGTKVTFKADKQIFNEIKFNRKTIVDHLRQQAYLTKGIS
                     ITFIDESEDIPFYYGFRFDGGIRSFIDYLNEGGDKAVHDNIFYISKEHENIEVETALG
                     YNEGIETEELSFANNIHTTDGGTHLTGLRSAITRTINDFARSNDYIKAKDENLTGDDV
                     REGLVAVVSIKIREPQFEGQTKAKLGNTEARTAVESVVGEAFKEFFERNTSDARRIVE
                     KTLLAAKARQAAKAAKETVLRKGVLEGLTLPGKLADCQSRKAEESELFIVEGDSAGGS
                     AKQARDRKTQAILPLRGKILNVEKSRIDKMLLNKEIRSLVIALGTAISEQFDISKIRY
                     HKIVLMTDADVDGAHIRTLLLTLFYRYFVPVIESGYLYIAQPPLYKIQSGKKFEYAYS
                     DGEKEKIVKSFDKENVNIQRYKGLGEMNPDQLWETTMDPAVRSLKVVAIEDAKEADRL
                     FDVLMGTEVGPRKHFIQVHASSVKNLDI"
     gene            complement(2723..>3046)
                     /locus_tag="VI432_02345"
     CDS             complement(2723..>3046)
                     /locus_tag="VI432_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY4496963.1"
                     /translation="KKQLKLGHKYEEVSKYPPITRDISFLVDSGFVPNTYFDLIRDIG
                     GDLVEEVALLDKYENKEKFGDKISYTYRVIYRSIERTLTTEEIEPIQQKITEETKAQF
                     GAEIR"
BASE COUNT          866 a          732 c          437 g         1011 t
ORIGIN      
        1 atcaacctcg gagatcttgc cgtcatagtc tttaaatgga ccgtcagtga ttctaaccat
       61 ctccccaacc gtcaaatcca ctttaaactt ggcttctttg cgtcccattc taaccatcag
      121 atcttctacc tcctcatatg ataggggcgc tggatttgta ggatctgctc cgacgaaccc
      181 cgttacgcgc ggagtatttc taaccacgta ccaagaatca ggctccatta tcatttcaac
      241 taaaacatag ccgggatata ttttttcttc taccgtgatt cttttgccgt ttctaatttt
      301 tatcttcttc tctttaggaa caatgatatt caaaatttta tcgttcatag ataacgattc
      361 aactctagtc tttaaatagc gcgcgaccgc atcctcatag ccagaataag tatgcacggc
      421 ataccagtgt tttatttttt ctttttccgg ttttaatgtt ttaaacattt tatacaacta
      481 attccaataa atatataaaa atataatccc aaagtcccaa aaagatcgag accataacag
      541 ataaagtgac aacgataaaa accatacgca ttgtctcttt acgcgtgggc caattaatcc
      601 tcttaaactc ttgacgcgat tcctgaagga aaagttttaa ttttgttatc atgtgattcg
      661 tatattaata ccacgagact atctatatat caaggttttt gacagatgaa gcgtgaacct
      721 gaataaaatg cttcctgggg cctacctccg tacccatcag aacatcaaat aatctatccg
      781 cttctttggc gtcttcaatc gcaacgactt tcaaactccg cacagccgga tccatagtag
      841 tttcccaaag ctgatctgga ttcatttctc caagaccttt atatctttga atattaacat
      901 tctctttatc aaaagattta acaattttct ctttttcacc atcagaataa gcgtattcaa
      961 atttcttacc agactgaatc ttatataaag gaggctgagc aatatataag taaccactct
     1021 ctataaccgg aacgaaataa cgataaaaaa gagttaataa aagagttctg atatgcgcgc
     1081 catcaacatc agcatcggtc atcaaaacta ttttgtgata tctaatttta gaaatatcaa
     1141 actgttcgct tatcgcagtc ccaagagcaa taaccaaaga tctaatttcc ttgttcaaaa
     1201 gcattttatc aatcctagac ttctcaacat tcaaaatctt tccgcgaagc ggcaatatag
     1261 cttgggtttt tctatctcta gcctgcttcg cgcttccgcc agcgctgtct ccctcaacaa
     1321 tgaaaagctc tgactcttct gcttttcgag actgacaatc agccaatttg ccgggtagag
     1381 tcaaaccttc caagacccct tttctaagaa cagtctcctt cgcggctttg gccgcctgtc
     1441 tggcttttgc ggctaaaagc gtcttttcaa ctatgcgcct ggcatcagaa gtgttcctct
     1501 caaaaaactc tttaaacgct tctccgacaa cggattcaac cgcagtcctt gcctcggtat
     1561 tgcctagctt agccttggtt tgcccctcaa attgaggctc tcttatttta atagagacaa
     1621 cagcaactaa accctctcgc acatcgtctc cggtaagatt ttcatcttta gctttaatat
     1681 aatcatttga ccttgcaaaa tcatttattg tccttgtgat ggcagatctt aaacccgtta
     1741 aatgcgttcc tccatctgta gtgtgaatat tattggcaaa acttaattct tctgtttcta
     1801 tcccctcgtt ataacctaac gcggtttcaa cctctatatt ttcatgctct ttgctaatat
     1861 aaaaaatatt atcatgaacg gctttatctc ctccttcgtt caaataatct ataaaagacc
     1921 taatcccgcc atcgaatcta aacccataat aaaaaggtat atcttcgctc tcatctataa
     1981 aagtaatgct tattcccttt gtcagatatg cctgctgtct taaatgatcg acaatcgtct
     2041 tgcgattaaa tttaatttca ttaaaaattt gcttatcagc tttaaaggta acctttgtgc
     2101 cggtcttctc tgatgatcca attttcttta ctctgttttt agccgctcct ctctcatact
     2161 cttgagaata aatccctccg tcacgattaa cctcaacctt aacccattcc gaaagagcgt
     2221 ttactacaga gaccccaaca ccatgcaatc ctccggagac cttatacgaa tctcctccaa
     2281 attttcctcc agcatgcagg gttgtcatga ctgtttctag cgccgaaact tttgtctgtt
     2341 tgtgcacatc tacaggaatt cctcttccat catcagaaac actaataacg ccatctttta
     2401 ataagcgaat ttcaatattt ttagcgtatc ctcccatagc ctcatcaatc gagttatcta
     2461 caacctccca gataagatga tgcaaaccgc taactccagt tgagcctatg tacatacccg
     2521 gccttttacg taccggttct aaaccctcta gaacgtagat gtccttagct gaatatcctc
     2581 cttgtccaga acccgctgaa ccggtttttt tctcttcttt ttttgattcg ttttttgcca
     2641 taaaaataaa atagccctcg cagaagctat gaatattatc acatatatag ggtattaaaa
     2701 caagctgata aacttaccca ttttacctta tttctgcgcc aaattgagct ttggtttctt
     2761 ctgttatttt ctgttggatt ggctcaatct cctctgtagt caatgttctt tctatcgaac
     2821 gataaataac tctataagtg taactgattt tatcgccaaa tttttcttta ttttcatatt
     2881 tatcaagcaa agccacttct tccaccaaat ctccacctat gtctcttatc agatcaaaat
     2941 aagtgtttgg aacaaaaccc gaatcaacca aaaatgatat atctcttgtg ataggaggat
     3001 atttacttac ctcttcgtat ttatgtccaa gttttaattg tttctt
//
LOCUS       DATDNZ010000061         6696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2791484, whole genome shotgun sequence.
ACCESSION   DATDNZ010000061 DATDNZ010000000
VERSION     DATDNZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791484"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..913
                     /locus_tag="VI432_02350"
     CDS             236..913
                     /locus_tag="VI432_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496964.1"
                     /translation="MISLIKKTADLKTLRGMATEHGNRLLDSAVRVACETHFKTHGES
                     TLMPWSGSFTIVVLGVDGNIIAAAANPTGPVYKEYGENLFQYALIKAVLRLHLERAGW
                     YTGLDNDENQAYLRELLPENEQIYLGEAKGEGLPIVGASGCELTHEYRNDIAPGIPPE
                     NVNFLAGYADQLFAEIVLVQFATNFEMAEKIGRIACDTSVQSALDRVKPEPVEEPSLF
                     AQLRRAH"
     gene            1775..2866
                     /gene="tsaD"
                     /locus_tag="VI432_02355"
     CDS             1775..2866
                     /gene="tsaD"
                     /locus_tag="VI432_02355"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY4496965.1"
                     /translation="MKILAIETSCDETSIAIIEASGSLKTTPKFRVLKNLITSQIELH
                     RKFGGVVPNLAKREHLINLPLLLQKLSVKSKLSDVDAVAVTIGPGLEPALWTGINFAE
                     DLSRKLNKPLLGANHLEGHLYSTLLKNNKKTILYPAIALIVSGGHTILLLLESLTKWK
                     KIGETRDDAAGEAYDKVARLLDLPYPGGPEIEKLSKLGDKNAINLPRPMLHQKNYDFS
                     FSGLKTSVLYRLRDMGYYKDPSIRKSASTRIKKIPQKIKNDIAASFQNAVVEVLTKKV
                     MKAAEKFKARTIFLSGGVASNRGLRNQLNKLCREHELKLLVPDFAFNTDNAAMIGAAA
                     YINTLKKSESLKKEIKSKYKIEAQGGLNL"
     gene            2905..5076
                     /locus_tag="VI432_02360"
     CDS             2905..5076
                     /locus_tag="VI432_02360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4496966.1"
                     /translation="MEPRYEHTSVEEKIYKKWIESGFFNPDNLPKENKKPFTIIMPPP
                     NANGRLHAGHALFITLEDIIIRYKRMRGFKTLWVPGADHAGFETQIVYEKKLEKEGRS
                     RFSSKGGSASGGKMSPDELFKEIMAFTLENKKYMEDDIKRIGASCDWSREKFTLDPDV
                     IEKTQKTFIKMHKDGLVYRGKRTINWCPKHQTSLSDVETENREEKAKLYYFKYGPFEI
                     ATARPETKFGDKYVVVHPDDKRYARWKHGDKLTVEWINGPIEATVIKDSAIDMAFGTG
                     AMTITPWHSQVDFEIAEKHKLDIEQIIDWRGKLLPIAKEFAGMRIEEAREKIVEKLKE
                     KGLLIKIDEGYSHIIKRCYKCANLIEPQIKDQWFVKMEPLAKLALKTVDDKKIKFIPE
                     NYEKIFRYWMENTIDWNISRQIVWGIKIPAWFHEPKCIPRKDHETQVIKCKDIIVSLN
                     KPECEFCDAEFIQDPDTFDTWFSSGQWPLLALGYPENNDFKNFYPTDVMETGYDLIFK
                     WVPRMVIFGLYLENKTPFHTVYLHGLINDAQGKKMSKSKGNVVSPIDLVDKYGADALR
                     MALIVGNTPGTDLALSEDKIKGYRNFSTKLWNIARFILTNRDETSIKPKFTEEDIKNK
                     KELIEIKDQVAKFMDDYDFNQSPNAIYHYVWHTFADKIIESYKPRLKSADLGNKKAAE
                     ETLLYILKESLKILHPFMPFITEEIWGMLPNNKNLLMIENW"
     gene            5078..5737
                     /locus_tag="VI432_02365"
     CDS             5078..5737
                     /locus_tag="VI432_02365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY4496967.1"
                     /translation="MILSPIILIILAVAPAIFWLIFFSIEDRKNPEPKPFVAFVFLAG
                     AAAALAALIPQLLIRKEFGLTNINLFTFETLVVFALIEEVFKFIAVYVTIYRSRFFDE
                     PIDAMIYMITGSLGFAAMENIFFLSAAGNGLINILIFRFIGAILLHALAGGLMGFYWM
                     RGQLWKGLTLAVLLHALFNYITLQQTNGLLYSIFLLFFASFFIFYDFDILKSKHGKRR
                     K"
     gene            5718..6098
                     /locus_tag="VI432_02370"
     CDS             5718..6098
                     /locus_tag="VI432_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY4496968.1"
                     /translation="MENEENKFNLEEEGEGQLTVDVYQTQDDIVVESTIAGIKPEDID
                     IEVTNESITIKGDRHREEAVSDRDYFYRECFWGKFSRSIILPQEVDPDKAHSSLKNGV
                     LTIKLPKIHRNKSKKLKIKSEHEA"
     gene            complement(6267..6626)
                     /locus_tag="VI432_02375"
     CDS             complement(6267..6626)
                     /locus_tag="VI432_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY4496969.1"
                     /translation="MIFDLYQVTNPGMLALRFAVAVIFIYHAMPKLKNYKGMGSGMGM
                     SSGFILLLGLTELSSALALITGYFMEIGAILLSMVMIGAITFKIKKWRVPFFAQNATG
                     WEFDFILLSANIAILLS"
BASE COUNT         2177 a         1412 c         1369 g         1738 t
ORIGIN      
        1 gcgtaaatag gggttcgatt ccccttggga ctaccaaaag aaactttttc agatgtaata
       61 acgtaatgtt gaatatattc tctatattga cttatgagtt aaaatatgtt atgttcataa
      121 ctaatcccga ggagggatct tactgacaca aggcacctta caaacccaac tgcaagaaag
      181 tctgcgcatt ttcgcgacag gcgaccataa ggagcgcgag agaacgctcc caccgttgat
      241 ctctctgatc aaaaaaacag cagatctcaa aacgcttcgc ggaatggcaa ccgagcatgg
      301 caaccgccta ctcgacagcg ccgtccgggt ggcctgcgaa acgcatttca agactcacgg
      361 agaatcaacc ttgatgcctt ggagcggcag ctttaccatc gttgtcttgg gagtcgatgg
      421 aaacataatc gcggctgccg cgaacccgac aggcccagtc tacaaggaat acggcgagaa
      481 tcttttccag tacgcgctta taaaagccgt actccgccta catttggaac gggccggttg
      541 gtacaccggg ctagacaacg atgaaaacca ggcctatctc cgcgagctac tccccgagaa
      601 tgagcaaatc tacctcggag aggccaaagg agaagggctg ccgatcgttg gcgcaagcgg
      661 atgcgaacta acccacgagt accgaaacga catcgcgccg ggaattccac ccgaaaacgt
      721 aaacttcttg gccggatacg ccgaccagct tttcgccgag atcgtcctcg tgcaatttgc
      781 aaccaacttc gagatggcag agaaaatcgg aaggatcgct tgcgacacct ccgttcaatc
      841 cgctcttgat cgagtaaagc ctgagccagt agaagagcct agcctcttcg cccaactccg
      901 gcgagcgcac tagctctcta tccgttcttt ttctttttta aacccgagca atcccatgct
      961 cggtgttttt gttttatata aaaatattct tcaaaaatga aacggccagg gaacgttagt
     1021 tcgctggcca tgtgttgtgg aaagtgatcc cccaccccga ccgaagccga gatgctctaa
     1081 tcaatgcttt tttcgtctga ccaggatggc catgagtgac tatccgatgt cgggtatgac
     1141 gtcggagaag gcattgatat caagctgctt actatacacg gtctagacgc cactggtcag
     1201 tcctgaagaa ccgacgttta gcatctccgc ttgcgagcag ggggactgtg aggaactcgg
     1261 tggaatttgc atccacaatt gccttgatcc cgcctctcga cgggcttggg gttgccttat
     1321 caaaaccaat gctaccaagc cgtttcccga aaagagaacg ccaagcgcat gcacatccac
     1381 tctcatctcc ccgactttcg tcgaagagtt ctgaactggc aatgccaaaa tccagatagc
     1441 tgaatttctc cagcgtttga gtcgcggact tgtaagtgcg atggtctgat tgcatatcca
     1501 gcatggatag ctcaagtctt taatctgtct gctactcaat gaagagtgtg cggacgactt
     1561 gattaggcga cgagtatcac tatgatttat gattgtgatt acaaagaact ataccttaac
     1621 ggtatttgcc atattattcc cctatagaaa agccgtcaag ggcaaaaatc caataaaatt
     1681 aatgattttg tgaacaatgt gtgtaatact tgtggaaaaa tatatatgtt tcttctttta
     1741 ttcggctaaa aagccgatta atatgtagtt acgtatgaaa atattagcaa tcgaaacaag
     1801 ttgtgatgaa acctctatcg ctattataga ggcgtctgga tctcttaaga ctactccgaa
     1861 atttagggtt ttaaaaaatc tcataacctc gcaaatagaa ctacatcgta aattcggcgg
     1921 agtagttccc aacttagcca aacgcgaaca tttaattaat ttaccgcttc ttcttcaaaa
     1981 attaagtgtt aagagtaagt tgtcagacgt cgacgcagtc gctgtgacca taggacctgg
     2041 ccttgagccg gcgctatgga ccggaataaa ttttgcagag gacttgtcgc gaaaattaaa
     2101 taaacctctt ttgggcgcga atcatctaga agggcactta tatagcaccc ttcttaaaaa
     2161 caacaaaaag accattctgt atccggcgat agctttaatt gtaagcggcg gacacacgat
     2221 attgcttttg cttgagagtc tcactaaatg gaaaaaaatc ggcgagacaa gagatgatgc
     2281 cgctggagaa gcatacgaca aagtcgcaag acttttagat ctcccttatc caggcggtcc
     2341 agagatagaa aaattatcca aactgggaga taaaaatgcg atcaatctcc cccgacccat
     2401 gctccaccaa aagaattatg attttagctt ctccggactt aaaacctccg tcttataccg
     2461 tctgcgtgac atgggatatt acaaagatcc aagcatcagg aaaagcgcga gtacgcgcat
     2521 aaagaaaatc ccgcaaaaga ttaaaaatga tattgcagcg agttttcaga atgcggttgt
     2581 agaagtttta actaaaaagg ttatgaaagc tgctgaaaaa tttaaagcca gaactatatt
     2641 tctttctgga ggagtcgcct ccaatagagg acttagaaat caacttaata aattatgcag
     2701 agagcacgaa ctaaaactgc tcgtgccaga tttcgcattc aatacagata atgccgccat
     2761 gatcggcgca gccgcatata tcaatacatt gaaaaagtcg gaaagtctta aaaaagagat
     2821 taaaagcaaa tataagatag aggctcaagg aggtcttaat ttataaattt tgcgttttac
     2881 attccgcacc ttaaacttac atatatggaa cccagatatg agcatacctc tgtagaagaa
     2941 aaaatatata aaaaatggat tgagtccgga tttttcaatc cggacaatct gcctaaagaa
     3001 aacaaaaagc cctttactat catcatgcct ccgccgaacg ccaacggccg acttcacgca
     3061 ggtcacgctc ttttcattac tcttgaagac ataataatac gctataagcg aatgcgaggg
     3121 tttaaaacat tgtgggtgcc gggagctgac catgcaggat ttgaaactca aatagtctat
     3181 gagaaaaaac ttgaaaaaga agggcgatcg cgattctctt ccaaaggcgg atccgcctca
     3241 ggcggaaaaa tgtctccgga tgaactattc aaagagatta tggcttttac gctagaaaat
     3301 aaaaaatata tggaggatga cataaaaaga ataggcgcct catgcgattg gtcaagagaa
     3361 aaattcaccc ttgatcccga tgttatagaa aaaactcaaa agactttcat taaaatgcac
     3421 aaagacggcc tagtctatcg cggaaaacgc acaatcaact ggtgtcctaa gcatcaaacc
     3481 tcattatccg atgtagaaac agaaaataga gaggaaaagg caaaacttta ttatttcaaa
     3541 tacggtccat tcgaaatagc taccgcaaga ccggaaacaa aattcggcga taaatacgtc
     3601 gtggttcatc cggatgataa aagatatgca agatggaaac acggagataa attaaccgtt
     3661 gaatggatca acggcccaat agaagcgacc gtcataaaag attctgctat agatatggcg
     3721 ttcggcaccg gcgcaatgac gataacgcca tggcattcgc aagttgattt tgaaatagca
     3781 gaaaaacaca aattagatat agagcagata atcgactggc gcggaaaatt gcttccaata
     3841 gcgaaagagt ttgccgggat gaggatagaa gaagctagag aaaaaatcgt agaaaaactg
     3901 aaagaaaaag ggcttcttat taaaattgat gagggttata gtcatattat aaagcgctgt
     3961 tataaatgcg cgaatcttat tgagccacag atcaaagacc aatggtttgt aaagatggaa
     4021 ccccttgcga aactcgcttt aaaaacggtt gatgacaaaa aaataaaatt catccccgaa
     4081 aattatgaaa aaattttcag gtattggatg gaaaatacaa ttgattggaa tatctcccga
     4141 caaatcgtct ggggtattaa aatacctgca tggtttcacg aacccaaatg tattcccaga
     4201 aaagatcatg aaacgcaagt aataaaatgc aaagatataa tagtttctct caacaaaccc
     4261 gaatgcgagt tttgcgacgc tgaatttata caagatccag acacgtttga cacttggttc
     4321 tcctccggcc aatggcctct gcttgcgctc ggatatcccg aaaataatga ttttaaaaat
     4381 ttctacccga ccgacgtaat ggagaccgga tacgacctta tcttcaaatg ggtcccaaga
     4441 atggtaatct tcggattata tctggaaaac aaaactcctt ttcacaccgt ctatctacac
     4501 gggcttatca atgacgcgca aggtaaaaaa atgtctaaga gtaagggcaa tgttgtaagc
     4561 cctattgatc tggttgataa atacggcgct gatgccctac gcatggcgct tatcgtcgga
     4621 aatacgccgg gaacagatct cgctctatca gaggataaaa ttaagggcta tcgaaatttc
     4681 tcaactaaat tatggaatat agccagattc atccttacga atcgcgatga aaccagcata
     4741 aaaccgaaat ttacagagga agacattaaa aataaaaagg aactgataga gataaaagat
     4801 caagttgcta aattcatgga tgattatgat ttcaatcaat caccaaacgc aatttatcac
     4861 tatgtctggc atacatttgc tgataaaata attgagagct acaaacctag attaaaatcc
     4921 gccgatcttg ggaataaaaa agctgccgaa gaaacactgc tatatatact aaaagagtcg
     4981 ctcaaaatcc tacatccttt tatgccgttt ataaccgaag aaatatgggg aatgcttcct
     5041 aacaataaaa acctattaat gatagaaaat tggtaaaatg attctttcac ctatcatact
     5101 gataatcctt gctgtcgcgc cggcaatatt ctggcttatt ttcttctcta ttgaagacag
     5161 aaaaaatccc gagcctaaac cgtttgttgc tttcgtgttt cttgccgggg ccgcagccgc
     5221 gcttgcagct ctcattccgc aacttttaat cagaaaagag ttcgggctca caaacataaa
     5281 tttattcaca ttcgaaactc ttgttgtatt cgctttgata gaagaggttt tcaaattcat
     5341 tgcggtttat gtgacgattt atcgaagcag attttttgat gagccgattg atgcaatgat
     5401 ttacatgata acaggaagtc tgggcttcgc cgcaatggaa aatatatttt tcttatctgc
     5461 ggccgggaat gggttaataa atatattaat attcagattt ataggagcaa ttcttttgca
     5521 tgctttggcc ggcgggctaa tgggattcta ttggatgagg ggtcagctct ggaaggggtt
     5581 gacgcttgcg gtcttactac acgccttatt caattatatt actctgcaac aaaccaacgg
     5641 actgctctat tcaatctttc tgcttttctt cgcttccttt tttatcttct atgattttga
     5701 tatacttaaa tctaaacatg gaaaacgaag aaaataaatt taatcttgaa gaggagggag
     5761 agggtcagct tactgttgat gtatatcaaa cacaagacga tatagtcgtt gaatcaacta
     5821 tcgcgggaat taaacctgaa gatatagata ttgaagttac gaatgaatca atcaccatca
     5881 aaggcgatcg acatcgcgag gaagcggtaa gcgacagaga ttatttctac cgtgagtgtt
     5941 tctggggaaa attctcccgt tcaatcatac tcccgcaaga agttgatccg gacaaagctc
     6001 attcatcttt aaagaacggc gtgctaacaa tcaagcttcc taaaatccat aggaataaga
     6061 gcaaaaaact aaaaatcaaa agtgagcacg aggcctaaat aaaaaacccc gcttaagcgg
     6121 ggtttttatc ttatccggtg catccgcagt cacaagtcgt acccgcctcg tgatcatgcc
     6181 cgcacatgga gcaagtattt gcttttcttc taatcattgt ttttatattt tataagtttc
     6241 gacgcataaa cttaaatcaa tgtttcttat gaaagaagta tggcaatgtt tgccgacagc
     6301 aggataaaat caaactccca tcctgtagca ttctgcgcaa aaaacggaac gcgccatttt
     6361 ttaatcttaa atgtaattgc tccaatcatc accatgctca aaagaatagc gcctatttcc
     6421 ataaaatagc ccgtaatcaa agcaagggcg cttgataatt ctgtcagtcc caaaaggaga
     6481 atgaaacccg aagacatgcc cataccagaa cccattcctt tataattctt aagtttgggc
     6541 atggcatgat aaatgaaaat aacagccaca gcgaacctaa gagctaacat tccaggattt
     6601 gtcacttgat ataaatcaaa aatcatatgt tttttattat acaatatcct tactaaaaat
     6661 gagagaggtc atttatctga tatatttctt acctat
//
LOCUS       DATDNZ010000062         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2796023, whole genome shotgun sequence.
ACCESSION   DATDNZ010000062 DATDNZ010000000
VERSION     DATDNZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796023"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VI432_02380"
     CDS             complement(<1..1639)
                     /locus_tag="VI432_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4496970.1"
                     /translation="MKLLLTSVFGPYGVDDEYGEKENKMELFHNQVTREQGIFSYRFN
                     HASHGLYFIAENVDVPTTVLDFPTLKRFKNELKKGYEYIGISFIIPNYQKAKKMAEII
                     REFSPDSKIILGSHGVNIPGIENMIEHDYICRGEGISFFRKLFNEDLDKSIKHPITYS
                     SFNRQVMGVPWPSGSGILITGVGCVNKCRFCATSHFFDNYTAYLKTGQDIFDVCCQFQ
                     EKLGVQDFGILDENFLKTPQRAIELLEVMEKKHRFFSFSIFSSAETLIELGDLDILVR
                     LGIKFIWIGVESKKEIYEKNKGANFSDLFKELKRRGISVMASAILFLEDHDKKSIWED
                     IEFATSLNADYLQFMQLGPIPGTALYKKYEEEGKLLKDVPYLAQHGQKQIWFSHDSFS
                     KEETEIILKNAFDYDYTENGASVFRAMDTTMQGYKYCKNHNDSRINSRGSDFKNMLKM
                     MRYFLLSARIFVENEASKNLLSKVRKNFNEQFGRIDLSSLLVSCLVILFSIKERLKIK
                     LFSDIRQPKELYKKFNFNKNKPSFVYHNLNDNNDLKLF"
BASE COUNT          701 a          476 c          411 g          687 t
ORIGIN      
        1 taaacaattt caaatcgtta ttatcattca aattgtgata aacaaaagaa ggtttgtttt
       61 tattaaaatt aaatttttta tatagctctt tgggttgcct gatatcagag aaaagtttta
      121 tctttagacg ctctttaatt gagaataaaa taaccagaca agatactaat aggcttgata
      181 agtcaatacg gccaaattgt tcattaaaat ttttcctaac cttagaaagg agattttttg
      241 aagcttcgtt ctcaacaaat attcttgcgc tcaagaggaa atatctcatc atttttagca
      301 tatttttaaa atcactccct ctcgaattta tgcgcgaatc gttatgattt ttgcagtatt
      361 tataaccttg catagtggtg tccatagcgc gaaaaacaga cgctccattt tctgtgtaat
      421 cataatcaaa cgcatttttc agaattattt cagtttcttc cttagaaaag ctatcgtgag
      481 aaaaccagat ttgtttttgc ccatgttgag ccagataagg gacatccttt aatagcttac
      541 cctcctcttc atatttctta taaagagcgg tgccaggaat aggacccagt tgcataaatt
      601 gcaaatagtc ggcattaaga gaggtcgcaa attcaatatc ctcccatatt gattttttat
      661 catggtcttc aagaaaaagt attgcagaag ccattaccga aatccctctt ctctttagct
      721 ctttaaaaag atcactaaaa tttgctccct tattcttttc gtatatttct tttttggact
      781 ccacacctat ccagataaat tttattccaa gacgaacgag aatatcaaga tcaccaagct
      841 ctataagagt ttcggctgag ctaaagatag aaaaagaaaa aaagcgatgt tttttttcca
      901 tcacctcaag tagctctata gccctttgag gagtcttaag gaaattctcg tcgagaattc
      961 caaaatcctg tacccccagt ttttcctgaa attggcaaca aacatcaaag atatcctgcc
     1021 ctgttttcaa atacgctgta tagttatcaa aaaaatgaga agttgcacaa aatctacatt
     1081 tgttgacaca accaactccg gtgataagaa ttcccgaacc ggatggccag ggcaccccca
     1141 tcacttgtcg attaaatgaa ctataagtaa tagggtgttt aatcgatttg tccaaatctt
     1201 cattgaaaag ttttctgaaa aacgatatcc cctctccacg acaaatataa tcatgttcaa
     1261 tcatattctc aatcccggga atgtttactc catggctccc gagaattatt ttactgtctg
     1321 gagaaaattc acgtatgatt tccgccattt tctttgcttt ttggtaattg ggtataataa
     1381 agctaattcc aatatattca tatccctttt taagctcatt tttaaatctt ttcaaggttg
     1441 gaaaatccaa aacggtagtt ggaacatcaa cattctccgc aataaaatat agaccgtggc
     1501 ttgcatgatt aaatcggtaa gagaaaatcc cttgttctct ggttacctga ttatgaaaga
     1561 gctccatttt attctctttt tctccgtatt catcatcaac accataggga ccaaaaacag
     1621 atgtaagaag taatttcatc tctattttgt tctcctctta tttcaaataa aaataaactc
     1681 aaaaatcaaa cagaagtttt ttatgatcat agtgaggcga accgatgtgc tacgaccggc
     1741 aggtatccgt gctcgttcag gcaaagcccg tagattggta aaggtgtggt caggattatt
     1801 aggcgggccc ggctgtggat tgaggcgagt taaataggac acagatttgc gcagattacc
     1861 gcagataaaa tcctgaaaat ctgcgttcat ctgcgtccaa aatgggaatt cctcaacgga
     1921 tcgagttaac gaagggaccg ggagttgtcc agggagctca ggatcattag acactcatta
     1981 ttcccggcaa taaaagcctc taaccctggt tccccaactg gaaagcctga tgcaagaccc
     2041 tcacggccaa ctcgatgtac ctgacctcaa tgacgccgga gattcgaaca aatgtatctc
     2101 cgtagggaga gaaagtcaag gggggtttta tgggcaatac ccgtgaatga atgagcaaac
     2161 actatagaac cttacattgt agtgtcaccg gacatgctgc acactccccg gccctatcct
     2221 ggacaggcga gccgcctgtc ccaccggtgg gtcgggcggc tcgcccgacc ggaat
//
LOCUS       DATDNZ010000063         4165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1760465, whole genome shotgun sequence.
ACCESSION   DATDNZ010000063 DATDNZ010000000
VERSION     DATDNZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760465"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VI432_02385"
     CDS             complement(<1..413)
                     /locus_tag="VI432_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4496971.1"
                     /translation="MILVKSLEKEYYSEDGRVLTNALRGIDLEIKSGDFLAIMGPSGS
                     GKSTLLHILSFLDRPTKGTYHFLDREISELSDEELASIRNKKMGFVFQAFNLLGRMSV
                     YENIEIPLMYSDTPADKRKNAIERVIKSVDLMDKIG"
     gene            complement(414..1952)
                     /locus_tag="VI432_02390"
     CDS             complement(414..1952)
                     /locus_tag="VI432_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4496972.1"
                     /translation="MVLSWQAKVLIFIFIAGGAVAFYLYLQIPGELATVEVVRGDIRS
                     EVEVTGRVHSPSSVNLAFESGGKIDEIYKIKGDRVNVGDLLTDLNRASLMAELRESEA
                     LIDIQQIKLNDLESGIRNIEVELYRSKVKSAEKVLADARKNLADRIYDAYLKSDDAIR
                     VKSANIFIDVQVFNPKLSFFIPDSSLKSELESLRIQAELKLKDWFNSVSQISDSSNIE
                     LKFEEASANTIFTRNFLDKEVAALTIASASASVSQSTIDTYLSNVSTARANLITAIIN
                     LTSANEKFRSAESALDIALKELAIQEAGFSDDDIAAQEAQLRQYVARRDVLLAEIAKT
                     KIISPIKGVITNIKFEEGEIVAANQGVITVISETEFEIEADVPEIDIGNIMRGDKVEI
                     TLDAFPGEIFMGHVGFIDPAETITDGVVNFSITVLFDKEDSRIKSGLTANLSIITDEK
                     VEILLVPGYVIIDRDAGRFVRKIQNGLESDVLIETGLRSLDGMVEVLSGLEDGDRVVN
                     IGIR"
     gene            complement(2022..2411)
                     /locus_tag="VI432_02395"
     CDS             complement(2022..2411)
                     /locus_tag="VI432_02395"
                     /inference="COORDINATES: protein motif:HMM:NF045193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496973.1"
                     /translation="MTLPSISAIAEEKGLNLENLSPENQEEILVEAMRRDLPLAAQFI
                     HAKAHEVMNGDRSTMWTEDPNSALGKQLIRMHASDALRPLASKYFAHGLPLTFMNCCG
                     GAVGNPPMDPMQMQIKMQAGPIAYADC"
     gene            complement(2489..3445)
                     /locus_tag="VI432_02400"
     CDS             complement(2489..3445)
                     /locus_tag="VI432_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4496974.1"
                     /translation="MRIAFVGKGGSGKTTLASLFARYLASQNRYVLAVDADINQHLGR
                     ALDFDELALESVPKMGLEMDKIKEYIKGDNKFIEHYSEIIKTTPPARGARFITPHTDN
                     PIVNYFSIVKDGINLMCIGEMEEEDIGTKCYHSKTGAAELFLNHLIDKNDHYIIFDMT
                     AGADLFASGLFTKFDLTLVVVEPTLKSINVYRQYKKYAKDYDVVIKAVANKINGDEDI
                     KFIRDNIGDDLVAVLGLSDFVKAYERGKVLSWEELESGNLEALFRIKQELDFSNRDWE
                     KLYRYAIDFHIKNAESWANKSLGIDLTKQIDPAFRISEIIGM"
     gene            3524..3973
                     /locus_tag="VI432_02405"
     CDS             3524..3973
                     /locus_tag="VI432_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY4496975.1"
                     /translation="MKKKTDKNSVKKLVRKAGLKTTPALISTLSLMRRTNEPLSTQEI
                     IEELNGKFDQATIYRIIHKLKHKGLINQVDLLHNHAHYELSDRKEHHHIICIKCGTVE
                     DIAGRNIQKMYENILDDSKYFAEINRHSLEFYGLCNKCGRSQLKVNI"
BASE COUNT         1331 a          950 c          648 g         1236 t
ORIGIN      
        1 ccaatcttat ccatcaaatc aacagatttg attactctct caatagcatt ttttctttta
       61 tctgcgggtg tatcagaata cataagaggt atttcaatat tttcataaac cgacattcta
      121 cctaaaagat taaatgcctg gaacacgaaa cccatcttct tgtttctgat agaggcaagt
      181 tcttcatcag ataattcaga aatctctctg tctaaaaaat gatatgttcc tttggttggt
      241 ctatccaaaa aactcaatat atgaagtaga gttgatttac ccgaaccaga cggccccata
      301 atagccaaaa agtctccgct cttgatctca agatcaatgc cgcgaagagc attagttaaa
      361 acccgtccat cctcactata atattctttc tcaaggctct taactagaat catttatctg
      421 atgccaatat taacaactct atcgccgtct tctaaaccgc ttaaaacctc aaccatccca
      481 tcaagacttc ttaatcctgt ttctattaaa acatctgact ctagtccatt ctgaatcttc
      541 cttacaaacc tacccgcatc cctgtctata ataacgtaac cggggaccaa aagaatctca
      601 accttctcgt ctgttataat cgataaattg gcggtcaatc cgctctttat cctactatct
      661 tccttatcaa ataaaaccgt tatagaaaaa ttaacaaccc catcagtaat agtttccgcc
      721 ggatcaataa aacccacgtg ccccataaat atttctcctg ggaaagcatc tagcgtgatt
      781 tcaactttat cgcctcgcat aatatttcct atatctattt caggaacatc agcctctatc
      841 tcaaattcgg tttctgatat aacagtaatg acgccttgat tcgcggcgac tatctctcct
      901 tcttcaaatt taatattcgt tattacgcct ttaatcggcg aaatgatttt ggtttttgct
      961 atctcagcga gcaatacatc acgtcttgca acatattgtc tcaactgcgc ttcctgcgcc
     1021 gcaatatcat catccgaaaa accggcctct tgaatagcaa gttcttttag cgctatatct
     1081 aaagccgatt ccgcagacct gaatttctca ttagcagaag tcagattaat gatcgctgtt
     1141 attagatttg ctcttgctgt tgaaacatta cttaaatatg tatctattgt agattgtgaa
     1201 acactagcgc ttgccgaggc aatagtcaaa gcagcaactt ctttatccaa aaaatttcta
     1261 gtaaaaatgg tgttcgcgct agcttcctca aatttaagct cgatatttga agagtcagat
     1321 atctggctaa ctgaattaaa ccaatccttt aattttaatt cagcttggat cctcaagctc
     1381 tcaagttcag atttaagaga tgaatctgga ataaaaaaac tcaatttggg attaaacacc
     1441 tgaacatcga taaaaatgtt agccgacttt acccggattg catcatcaga tttcaagtat
     1501 gcatcgtata tcctgtctgc taagttcttc ctggcatcag ccaaaacctt ctccgcgctc
     1561 tttaccttag atcgatataa ttcaacctct atgtttctaa tccccgattc aagatcattt
     1621 aatttaatct gctgaatatc aattaaagct tcgctttcac gaagctccgc catcagactc
     1681 gccctattca aatcagtcag caaatctccg acattaactc tatccccttt tattttatat
     1741 atctcatcaa tcttgcctcc actctcaaaa gcaagattaa cgcttgacgg agaatgcact
     1801 ctcccagtca cctcaacctc gcttctaatg tctcccctca ccacttcaac tgtcgcaagc
     1861 tctcccggga tctgcaaata taaataaaaa gcaaccgcac cgcctgcgat aaaaatgaat
     1921 attaaaactt tggcttgcca gcttagaacc atatctgatt attataaatc aaagggggaa
     1981 agaaataaaa aaagagcccg aattatcgag ctcaaaatgt actagcaatc cgcgtaagcg
     2041 ataggtccgg cctgcatttt gatctgcatc tgcatcggat ccatcggcgg gttacctacc
     2101 gcgccaccac agcaattcat gaaagtgagt ggcaaaccgt gagcgaagta tttagaggca
     2161 agcggacgaa gagcgtcaga cgcatgcatc ctaataagct gttttccaag agccgagttg
     2221 ggatcctcgg tccacatcgt agacctatcc ccattcatca cttcatgcgc cttagcatgg
     2281 ataaattgcg cagccagagg caaatcgcgc cgcatcgctt ccaccagaat ctcctcctgg
     2341 ttttcgggcg aaagattttc aaggttcaaa cctttctctt cggcaatcgc cgaaatagag
     2401 ggtagcgtca tacagacatt ctcctgctga aatatgatca ctcatatcca gcgctaaaaa
     2461 ttatattatc cttagcgctg gatacgaatt acattcctat aatctcgctg attctaaaag
     2521 ccggatctat ctgtttcgtc aaatcgattc ctagagactt attagcccaa ctctcggcat
     2581 ttttaatatg aaaatcaatc gcatatctat ataatttctc ccaatctcta ttagaaaaat
     2641 ctagctcttg tttaatccgg aaaagcgctt ctaaattgcc actctctaat tcttcccagc
     2701 ttaaaacctt accacgttca taagccttaa caaaatcaga caaaccaaga acagctacca
     2761 gatcatcgcc tatattatct ctaataaatt ttatatcttc gtctccgtta attttattag
     2821 caacggcttt aataacaaca tcataatctt tagcgtattt tttatattgc ctgtaaacat
     2881 taattgattt cagggtcggc tctacaacta caagagttaa atcaaattta gtaaaaagtc
     2941 cggaagcaaa gagatccgct ccggcggtca tatcaaaaat aatgtaatga tcatttttat
     3001 ctataagatg attcaagaaa agctcggcgg cgccagtctt agagtgatag cattttgttc
     3061 caatatcttc ttcttccatt tcgccgatac acatcaaatt aattccatct ttcacgatag
     3121 agaaataatt tacgatcggg ttatctgtat gcggcgttat aaaccgcgcg ccgcgggcgg
     3181 gaggagtagt tttaataatc tccgaatagt gttctataaa tttgttatca cctttaatat
     3241 attcttttat tttatccatt tctaaaccca ttttaggaac tgactctaac gcaagctcat
     3301 caaaatctaa cgcgcgccca agatgctgat taatatcagc gtcaacagct aaaacgtatc
     3361 tattttgaga tgctagatag cgcgcaaata aagacgccag agtagtcttg ccgctgcctc
     3421 cttttccaac aaaagcaatt ctcatattgt aagaattgtc tccataatta atctaatggt
     3481 caagttattt gcgaataatt cgcatttaga ttagagtggc cacatgaaga aaaaaacaga
     3541 taaaaactct gttaaaaagc ttgtgagaaa agcggggtta aaaactacgc ccgcccttat
     3601 ctctactctg tcacttatga ggcgtactaa tgagccatta agcacgcagg agataataga
     3661 agaattgaac ggcaaattcg atcaagcaac tatctatcgt attattcata aattgaaaca
     3721 caaaggctta ataaaccaag tcgacctgct tcacaaccac gcccactatg aactatccga
     3781 cagaaaagag caccatcata taatttgtat aaaatgcgga acagtagaag atatcgctgg
     3841 acgcaatata caaaaaatgt atgaaaatat attggacgat tcaaaatatt tcgctgagat
     3901 aaatcggcac tcactcgaat tctacggact ctgcaataaa tgcggccgat cacaacttaa
     3961 agttaacatc tgacagctaa caaataattt tttcaattta cgagtgaaag ttatccacag
     4021 gcaaaagaaa gaaaatatgc gaataatttt gtaaaattaa tacagtaagt taatttttta
     4081 aattttgtat caaaaacttt aattatcaaa ttaaggtttc cggcgtaaag gtcgataaaa
     4141 acttttaata agacacaaat ggcaa
//
LOCUS       DATDNZ010000064        19886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3354691, whole genome shotgun sequence.
ACCESSION   DATDNZ010000064 DATDNZ010000000
VERSION     DATDNZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354691"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VI432_02410"
     CDS             complement(<1..471)
                     /locus_tag="VI432_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496976.1"
                     /translation="MKFLKNFLIMVSVIVVAQSNSADGAYVNEYVQSLIPGGQQGISD
                     VFNVVDSGFLDISINSSETVHMRIESVPNMIVMDIEASPYTESTEITINNLLPLTKYY
                     KYEDSHRDLEIITTDENGLYFYVQDLKSPHLVFIKTLPSTKYIKDDSTGGDCELI"
     gene            complement(496..1353)
                     /locus_tag="VI432_02415"
     CDS             complement(496..1353)
                     /locus_tag="VI432_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY4496977.1"
                     /translation="MMKAIDYIRKAEKEGIAIGHFNISDVVALRAIFDAARELRVPVF
                     IGVSEGEREFIGIRQTKALVDSLREEYNYPIFLNADHTYSFEKIKEVIDSGYDSVIFD
                     GAKLDFEENVKRTKEVVEYARSRNPEILVEAELGYIGSSSKIYDKIPEGAGVRPTTPE
                     EAKQFVEETKIDLLAPAVGNIHGMLKGVKNPRLDIDRVRAIQDAAGVPLVLHGGSGIE
                     DEDFKNAIKAGISMVHINTEIRKAWRDGVEAGLRDNPDEVAPYKVLPPAYEGIKKVVL
                     ERLRLFSGI"
     gene            complement(1377..2609)
                     /locus_tag="VI432_02420"
     CDS             complement(1377..2609)
                     /locus_tag="VI432_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY4496978.1"
                     /translation="MVRGVKNGFTLIELLIVVGILAVIGTATVFVINPVELLARSRDS
                     RRLEDLNKLSTTLQVYETQAGALGGSPNTVYISLPNTLSDCTGAPTYTLPSLPSGWSY
                     NCATEANFRNINGDGWIPTDFTRAPGLGLALLPIDPINSDTDQLYYTYIKGSWNITAK
                     MESNKLSQTTASTDNGTDPTRYEKGSDLNVWNQVSGLVGYWKLDDGSGTVTVDSSGND
                     KNGTLNNGPIWSSLGRIGGALSFDGVNDYVSFSSGSLFNIIPGEVKTFTIWFKGSLDS
                     SRDSTILWQDGNCIGWSSHMSSAGGLRAVLHTGNSGCTGYSTYSLNSTINYDDDKWHF
                     AVTVVDRPNLLMTLYVDGVTRGTVAIDNALSGTGGSARIGTNWNNTTPFNGLVDDIRI
                     YNRALSASEIEAIYNATK"
     gene            complement(2594..3862)
                     /locus_tag="VI432_02425"
     CDS             complement(2594..3862)
                     /locus_tag="VI432_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496979.1"
                     /translation="MSEVSDKLARILRIDKNIFADLQDSLAASTGKIGIIDKIAIENE
                     VLVFKKLEELGLKRNSYASNVYEALIVKLAEDDRKVYEAAGLGVGIEGQAAAQKICDF
                     IAKVLPPRNGWFIKKEKAREFLLNIPPMHIVKGLGYNTASEMLEKEDLLEVFSALRFL
                     EDGEWLNNVFFKQYENLSPDDFEERPIEIRALNNKWAVAAEKFVKKKYHNVSHLKEMG
                     VIFVIPIFMNMSGETLRLVSLLAHYLNEVKYYSDLFVSFSKEEKFADSIISLLRGDVA
                     EERGLMHAEEVTGSRFLVIQRYLAKGDEHDWRLFVPHVNPEAVHWSRAEDAIVRIPEV
                     YPDFKNHLDFWRGLGWVGDYFGTESGLDVLVSFNIVDTVMSLVKKHELIKYLYHHQEA
                     LWNRIFIEYYGEEKMMQLIRENIVKGWFEV"
     gene            complement(3878..4888)
                     /locus_tag="VI432_02430"
     CDS             complement(3878..4888)
                     /locus_tag="VI432_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY4496980.1"
                     /translation="MHDIITIGSTTYDSYYRINFKTTPWKTPTGKAYIIPTGEKFGIE
                     DAYATLGGNAANAAVTFARHGFKTAVMARIGFDAEGEEIKKRLKKERIETKFIKHSLK
                     AYTARSVLLLQKGERTILSYHGAINEFSLKDIDFKNLKAKWIYVSLPGDSYTIFSKLV
                     REASKRGIRIAVNPSFKHLSEGKKKLLQDLKYVSVLFVNDGEASELTGIPFKQRERML
                     KKLTSLINGIAVITLGPEGVIASDGRRIYSAGIFKEKMLADRTGAGDSFGSGMVAALM
                     DGGDKKLFDSRNIERAIRFATANSTSNVEHIGATEGVLTKNEFDKSSRWRNLKIRDRI
                     IN"
     gene            complement(4940..5941)
                     /locus_tag="VI432_02435"
     CDS             complement(4940..5941)
                     /locus_tag="VI432_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4496981.1"
                     /translation="MLNKFAKLNPKIFSDDVENVPIRNGYGEGLIIVGEKDENVVVLC
                     ADLTESTRSEAFSQKFPNRFFEMGVAEQNMATIAAGLGISGKIPFISSYAAFSPGRNW
                     EQIRTTISYNNSNVKIAGHHAGISVGPDGATHQAVEDIATMRVLANMKVFVPCDAIEA
                     RKATIAAAQIAGPVYLRFAREKTPVITTDGSPFVPGKADIMWDSSSQRKKADVVIIAC
                     GPLVYNAIIAAKELEDEGIKTIVLNNHTIKPMDEKKIIELAKKTGAVVTVEEHQVAGG
                     MGSAVAEILAKHVPTPQEFIGLQGVFGESGPPKDLIEKYGMGVKSIIKAVNKVIKRK"
     gene            complement(6056..6916)
                     /locus_tag="VI432_02440"
     CDS             complement(6056..6916)
                     /locus_tag="VI432_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4496982.1"
                     /translation="MDSIHEEKIKILEERANIIRQDIIKTLLEAGSGHSAGPLGMADI
                     FTAFYFHILDHDPENPDWEDRDRLILSNGHICPVRYVTMAHAGYFPLEELKTLRKLNS
                     RLQGHPHRTALPGVETTSGPLGSGLGQSVGMALAAKLNNKKYRVYCLMSDGEHNSGNT
                     WESAMLASKYKLNNLTAVIDRNNIQIDGYTENIMPLEPLRDKYESFGWHVLNIDGHNM
                     EAIVDAVNEAKAIYEKPTVIIAHTIPGKGVSFMEGDFLWHGKPPKKEEADKALKELRT
                     LEGQIESEHE"
     gene            complement(6942..7592)
                     /locus_tag="VI432_02445"
     CDS             complement(6942..7592)
                     /locus_tag="VI432_02445"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY4496983.1"
                     /translation="MFVIPAINCGDYKTAEEQIKTAVSFIGQGGWIHIDIVDGKFAPN
                     FTWGTPEELGRIIRDNSLFDINFEIHLMVKNPHDFAEDWFRVGAKRLIVHLETLENLQ
                     SVIDSAKKHNGELMLAIDQDVKIDDAVPFLDKFDYFQILAVRPGLAGQKFIPDSLYRI
                     KFLRQRAPNVKIEVDGGINSETARLCKEVGADIVVSASFIFESGNPEQAYKELLAT"
     gene            complement(7696..8400)
                     /locus_tag="VI432_02450"
     CDS             complement(7696..8400)
                     /locus_tag="VI432_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496984.1"
                     /translation="MVDFEVLPWEVKEGRVTEIRVLHLGLKEAVRLRPKEPIAPDELS
                     SLKSAVKAFFEAREAFVGDKPPVIEDATTECLQRLASKRAPAQGTNGASHSLVSRDPG
                     DQLVEVSDHVWSKMPPFVQNRDGLRTSVTRRDLQTVIDVLPSVNETTVLKDLGEKSGI
                     SAPKLRLIVGVLAQSEIVSVHEVNPKLIKVTTLEKPNMLADHSGIPVIESDDPGLARA
                     MASMREGQNVLLGTMS"
     gene            complement(8556..9008)
                     /locus_tag="VI432_02455"
     CDS             complement(8556..9008)
                     /locus_tag="VI432_02455"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEY4496985.1"
                     /translation="MLIYLGSDHRGFKLKEFIKTYLKDNGYQIHDVGNVKYDPADDYP
                     DFAKAVAENVAADPDGRRGVVFCGSGVGVDIVANKFDRVRSVLANTPDQVMVSRVDDD
                     TNVLSVAADFINPEDAKRMIAIWLQTPFSKEERHKRRLDKIGEIEKNN"
     gene            complement(9008..9421)
                     /locus_tag="VI432_02460"
     CDS             complement(9008..9421)
                     /locus_tag="VI432_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEY4496986.1"
                     /translation="MYTLDINFVVVILAAIASMALGMIWYSPIGFGKKWMSLVGVSVD
                     AKPKGAMKSYFLSFFAAIIMAYVLSLFIINAPPVSISSAIQIAFWVWIGFVATTNISE
                     YLFSPTPKPMALFVLNAGYHLASLMTMAIVISIWG"
     gene            complement(9431..10444)
                     /gene="gap"
                     /locus_tag="VI432_02465"
     CDS             complement(9431..10444)
                     /gene="gap"
                     /locus_tag="VI432_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY4496987.1"
                     /translation="MTRVAINGFGRIGRLFFRQAFGLSDIEIVAINDLGDAHNLSYLL
                     QYDSVYRKYDKSVEVRDGGLVVNGKDIKFLQIKDPTTLPWKDLNIDVVVESTGVFESY
                     EKAKMHLDAGAKRVVISAPAKDDDGELGKTVLLGANDSEINVCSINSNGSCTTNATSP
                     VVSIMNETIGIQKALLNTVHAYTATQNIVDGPVRGSDYRRGRAGAQNIAPSTTGAAIA
                     ITRVIPDLEGKFDGISFRTPTITGSAANITFLAKRKTTVEEINKIFKNAAKDPRWDGI
                     LYVTEDQVVSSDIIGNTHAAIIDLKFTKVIDGDLVNVLSWYDNEYGYVSTLVKHVQKV
                     VIV"
     gene            complement(10640..11827)
                     /locus_tag="VI432_02470"
     CDS             complement(10640..11827)
                     /locus_tag="VI432_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4496988.1"
                     /translation="MDKSDKKIFGLRKNVFFLGLVSLFNDFSNEMIQSVMPLFLTSIL
                     GNTAVLALGIIEGVADALSSFVKVASGVVSDRVGRRKPFAILGYSLSVVTRPFLGLAS
                     AFSHVFSLRLLDRFGKGLRDAPRDALLASSVKDADLGKSFGYQRSMDALGGLLGPLAA
                     LAILPLINNNFPTLFIISFIIGLLALGSFFWVREIRGAGMISNNKHLKENLFKTHKHF
                     TLFIIAVFVFGLGILPIGLILFRVGELGLGDQFVPLLYFIYGLVFTLSSAPLGRLSDR
                     VGPVKVIVIGFILAITAYIILILTASLWGLILALMALGVYSGATDGIQRALAAKLMGH
                     ELLGAGEGILQASIGLSSLFAGTVGGLLWFSFGHAVALSYVVAVSASGLVMLLLINRR
                     HKI"
     gene            complement(11840..12214)
                     /locus_tag="VI432_02475"
     CDS             complement(11840..12214)
                     /locus_tag="VI432_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY4496989.1"
                     /translation="MDKFCVANIKGSDFKLTTADNFIKRAIGLAWQKDVRDGADGMLF
                     AFKNKARRPFWMFGMRFDLDILWIDNDEVVGFEKLVPHTGLVNQIRLHRSKEPVDKVL
                     ELKPGVIDFLEIEIGDKITIHL"
     gene            complement(12217..19845)
                     /locus_tag="VI432_02480"
     CDS             complement(12217..19845)
                     /locus_tag="VI432_02480"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4496990.1"
                     /translation="MLEQKIGYKPSFSDRIYRFALKHPGLIYLGPIFFLTLIFTYAVI
                     SLMGIYVGAWALLPVSIIIFILNSDIAVNLVNSVLSRLTPVRLLPKIDYVVNDIPDKD
                     ATLVVIPSVFRSKILIGNLLRKLEVHYLGNRDKNIFFGLLMDFRDSKNEINKEDSEYI
                     KFLENGIDALNKKYKSAGSRDRFFSLYRHRIFNEKDKVFMGWERKRGALREFNKLILG
                     DEDVTYAIPKNGLNVPSVRYVVTLDDDTEVPQDTIKKMIGCISHPLNKPILKRDEGRV
                     SRGYGIIQPQIATRIDSAKYSLFSNAFAFSIGLDSYSRPSPDVYQDLFGNAIFFGKGI
                     YDVEVVEETMKDNIPENRVLSHDLLEGLYAKTGLSSDIQVFDGFPQNYNEYLTRLHRW
                     IRGDWQIIHWLRPNFLRVKDGMRGNRFGVDDKWRILDNLRRSLIPPFSLVIIFFILFS
                     PLHNVWDIYPIIILGSPFVIVFFAESFSFFRTFPFIDAIKMVVYKSFVVILQIFTRLS
                     LLFQQSIVYVDAIARAVSRLFITKKKLLQWTASQEAAKENRGGLIRFYKSMWLTPFFS
                     GAMSLILYFLGFGGVAAYVWLSLWFLSPFIAYILSLPRHKKYKPRESDINFLRGIAYR
                     NFRYFIEFSNQSNNWLIPDHLQEYPRVARTEYKVTTSPTNLGALINSLMIGYELGYIS
                     FERFINNLTKTFHTLSSLERFKGHFYNWYDVKTLKPLFPKYISSVDSANMLANMLVLQ
                     GSLKELRRDKILGNNLQAGFLDALGVLREDLHTLFRSPSAAQGAKKYIKKISNNLNKI
                     IRSISVRNTKKLSGFLNILNEIALFNSDTRSRLAGLEFTGGEEFFVGIEKSLKFLEEL
                     VEECYRTFQSGASFYKVFSNIPVRKEIVELRDFYALIAKIIDKVPSIGDFEADRLEIE
                     LDSVRLDSLSSAGVSKEEIVLLRSWIQNIRDEIKKSKAYALSLIKEASNISENALKIF
                     NGTEFKFLYNKERGLFHIGYNASFNKIDNAYYDFMASEANLISFIGILKGDVPQKHWF
                     HLGRKLVRVGNKGVMLSWGGSLFEYLTSLLFLNVHPESLIGETARTAIKTHRNYARLF
                     AMPWGIGESAYNMFDASQSYQYQVFGVPILGLKRGLKDYRVVAPYTTALALRFYPKIA
                     IRNFKRLGRKGVLGIYGFYDAFDYTGTTGFKPASGIPVKIYYAHHQGFTLAGISNLIQ
                     DGRMIKLFHMDPRVESISALLEEGAPRTIPLRPVKSASIVPKKKTPDTNLISKYIPIK
                     TRIPRYALLSNGNYFLAVSNSGAGYSKYQDIQLTRFKDDPVLEEWGSFIYMKDLDTSE
                     LWSPTFQPMRKGGKKNKIIFHENRVEFRQFYNNIESNLRVFIPSVENMEIRELSLTNA
                     GDVVRNIEVASFGEVVMTKPGADKDHPMFHKLMVESEFIARHRTLLFNRRHPSDRSRK
                     IYFAHSVILPRHAELMGYARSREDFLGRNGSISKPAFIHNNKIKKDLEPKHTLDSTFS
                     LNAKISIKPGDTAKIVFINSVGSAKEEIMRLIKKQTKNYSSRKVIHENNNLSIKTLKD
                     INISAEDAILFQSLASRIMVGEIKPMPSVARERKSIIESLWRHGISGDFPILSVRVKD
                     IEDAPFVKQILLFNQYMRYKGLDVDIVVLNEHPSSYIRTLDDEIDFMIRSNRAAREDS
                     GGGGVFHLKSDLVSDIDKEILLFASRFILDSKSGALSDQISVRNGYGRTIFGDRLGSG
                     IKPSLDKPNISLDVETQLFNGFGGFDSKDGSYVFLTGADDNTPSSWANIVTNDKFGFL
                     MRENGSSFTWSEDSYDNRLTALNRDEYRYGSGEIFYIKDDETGEVWSPTPYPSYGRHL
                     YRVTHGRGFSSFDHNRNGIEHKFLSYVPVDDPVKIVNVKLKNLSSKSKKLSLVGLFEP
                     VLGSNKEDTRHVLDIKYNSSVKAVLVENKFRSHLRDRIAFFDMAGGNFVYSFNREHFL
                     GRHGSINYPSALHRERIDKGQEDLGENIIDNCIALQAGVDLAPGEEKEIIALIGEGTT
                     KDEVKNYLSKYRSKIRSATSLSRAIAKWENGPFKIDCPDKSLNMLFNDWLLYQILSSR
                     IMGKTGYYQSSGAYGFRDQLQDSMALIYREPALVRQLILKAAKHQFKEGDAQNWWHDH
                     NNFGIRTAFSDHQLWLPYVITSYINITGDESVLEEREEFLDGPRVNFIDRLEWVGVPD
                     AKTDRYSIYEHCIRAINHTMKFGPNGLPLFGVSDWNDSLNKVGVEGKGESVWLGWFLY
                     ATLMRFSNLSEKRGDYKNTERFRAAASGLQRSLEEEAWDGSWYKRAFMDNRIPLGSSQ
                     NQEFKIDSIAQSWSVLSGGGKNERAKRAIESALGNLVDGDVVRLLAPPLQSVVFDPGS
                     IKDYPPGVRENGGQYNHAALWLAQALFVLGDGDSGKKILDLINPALRSQKDVARYRVE
                     PYVLSSEVYAPPSYDGRGGWTWYTGSAGLFYRTILEFIFGLNISKDTMTIDPCLPKDW
                     DKCSISYKLGNTEYLITIYNPNGVNKGVVSVKIDGRDNKSNTINILNDGRRHKIVVKL
                     GFKE"
BASE COUNT         6113 a         4645 c         3251 g         5877 t
ORIGIN      
        1 tatcaattcg cagtctccac ctgtagaatc gtccttaata tacttagtgc tcggtaaagt
       61 tttaataaaa actagatgtg gggacttcaa gtcttgcaca taaaaatata aaccgttctc
      121 atcggtagta atgatttcta aatcgcggtg actatcctca tatttataat attttgtaag
      181 aggtaataaa ttattaatag taatttctgt tgattctgta taaggcgacg cctctatatc
      241 catcactatc atattgggca cagactcaat tctcatatgt acggtctccg aactgtttat
      301 agaaatatcc aggaaaccac tatcaacaac attaaaaaca tcagagatac cttgttgacc
      361 tccaggtatt aaagactgaa catattcatt aacatatgct ccatctgccg aattactctg
      421 agcaacaacg attaccgaaa ccataatcaa aaagtttttt aaaaatttca tatgtcttta
      481 tgaatacaat aaaaattata tcccgctaaa caacctcaat ctttctaaaa ccactttttt
      541 aatgccctca tatgcgggtg gaagaacttt atatggagca acttcgtcgg gattgtcacg
      601 caaacctgcc tccacaccgt ctcgccacgc ctttctaatt tcggtattaa tatgcaccat
      661 agagatacca gccttaatcg catttttaaa atcctcgtct tcaatgccgg atcctccatg
      721 caaaacaagc ggcacgccgg cggcatcctg tatcgctctc acacgatcta tatccaaccg
      781 cggattttta actcctttta acatgccatg gatattgcca acagcggggg caagtaaatc
      841 aattttagtt tcttcaacaa attgtttcgc ctcttccggc gttgtggggc gcacaccagc
      901 gccttcaggg attttatcgt aaattttgga agaagagccg atataaccca attcagcctc
      961 taccagaatt tccggatttc tagatcttgc atactcaaca acttcttttg tgcgcttaac
     1021 attttcttca aaatccaatt ttgccccgtc aaaaatcaca gaatcatatc cagaatcaat
     1081 cacttctttt attttctcaa acgaatatgt atgatcagca ttcaaaaaaa tcgggtaatt
     1141 atattcttct ctcaaactat caactaacgc ctttgtttgc cttatgccaa taaattccct
     1201 ctcaccttca gagacaccaa tgaaaaccgg aaccctaagt tcgcgggcag cgtcaaagat
     1261 ggcgcggagt gcgacaacat ccgagatatt aaaatggccg atagcaatgc cttccttctc
     1321 cgcctttctt atataatcaa ttgctttcat cattcaaaat tatatcagaa ttctaattat
     1381 ttagtcgcat tataaattgc ctcaatctca gaagcagaaa gtgcacggtt ataaatacga
     1441 atatcatcaa ccaagccatt aaatggagtt gtattattcc aattcgtgcc tattctagca
     1501 ctccccccag taccacttaa tgcattatca atcgccacag tccctctagt aacgccatca
     1561 acatataaag tcattaataa attcggccga tcgacaacag tgactgcaaa atgccactta
     1621 tcgtcatcat aatttatggt tgagttcaaa gaataggtag agtaacctgt gcagccgctg
     1681 ttgccagtat gtaagacagc cctcaatcca ccagccgatg acatgtgtga agaccaacct
     1741 atacaatttc catcttgcca taaaattgta gaatctctag aagagtctaa actgccctta
     1801 aaccaaattg tgaatgtctt aacttctccc ggaataatat taaaaaggct tccgcttgaa
     1861 aaactgacat aatcattaac gccatcaaaa cttaacgcgc ctccaatccg acccaacgat
     1921 gaccaaatcg gcccattatt aagtgttccg tttttatcgt tacccgaaga gtcaacggtt
     1981 acagtgccag aaccatcatc aagcttccaa taccccacaa gtcctgaaac ctgattccaa
     2041 acatttagat cagatccctt ctcgtaacgg gtggggtctg tgccgttatc agttgatgcg
     2101 gttgtctgcg agagcttgtt cgattccatc ttggcggtga tattccaact gccctttata
     2161 tatgtgtagt agagctggtc ggtgtcggaa tttatcggat caataggtaa aagagctaaa
     2221 cccagtcccg gggcgcgagt aaaatcagtt gggatccaac catcgccatt tatatttcta
     2281 aaattagctt cagtagcgca attataactc cagccagacg gcaatgaagg aagcgtataa
     2341 gtcggtgcgc ctgtgcagtc cgataaagta ttgggaaggg agatgtatac agtattagga
     2401 gaccctccca aggcacctgc ttgggtttca tagacctgta gggtagtgct taatttgttt
     2461 agatcctcta gtcttctgga gtctctgctt ctggcaagca gttcaactgg attaattacg
     2521 aaaaccgtag cagttccgat gacggctaag atgccgacta caataagcag ctctatgagg
     2581 gtgaagccgt ttttcacacc tcgaaccatc cttttacaat attttcgcgg attaattgca
     2641 tcatcttttc ttctccataa tattctatga atattctatt ccatagcgcc tcttgatgat
     2701 gataaagata tttaataagc tcgtgtttct tgactaagct cattacggta tccactatat
     2761 taaaactcac caaaacatcc aagccggatt cagtaccgaa ataatcgccg acccatccga
     2821 gtccgcgcca gaaatccaaa tgatttttaa aatcagggta aacctccgga atcctaacaa
     2881 tagcgtcttc agctcttgac caatgaaccg cttccggatt cacatgcgga acaaacaatc
     2941 tccaatcatg ctcatctcct ttagctaagt atctttgaat aaccaaaaac cttgagcctg
     3001 tcacctcttc cgcgtgcatc aaccctcttt cctcggccac atctccacgc aaaagagaaa
     3061 ttatgctatc agcaaacttc tcctcctttg aaaaagaaac aaaaagatct gaataatact
     3121 taacctcatt caaataatgc gcgagcaaac tcaccagcct caaagtctcc ccagacatat
     3181 tcataaaaat aggtataacg aaaataacgc ccatctcttt tagatggctc acattatgat
     3241 attttttctt tacaaatttc tccgccgcta cagcccactt attattaaga gcccttattt
     3301 ctatcggacg ttcttcaaag tcatcaggac tcaaattctc atactgttta aaaaatacat
     3361 tattcaacca ctctccatct tctaaaaatc taagggcgct aaaaacttcc aacaaatctt
     3421 ctttttcaag catctcagag gccgtattat atccaagtcc ctttacgata tgcatcggcg
     3481 gaatattaag taaaaattcc ctggctttct cttttttaat aaaccagcca ttccgcggag
     3541 gtaaaacttt agctatgaaa tcgcatatct tttgcgcagc cgcttgaccc tctatgccga
     3601 cgcctaaacc cgccgcctcg taaactttcc tatcatcctc ggcaagtttt actatcaacg
     3661 cctcataaac attactagcg tagctattcc ttttcagtcc taattcctca agtttcttga
     3721 aaactagaac ctcattctct atcgcaattt tatctataat tcctatttta ccggtggacg
     3781 cggccagact atcctgcaaa tcggcaaaaa tatttttatc aattcttaga attcttgcaa
     3841 gtttatcgct tacttcagac atttttataa attaatatta attgattatt ctgtccctta
     3901 tttttaaatt gcgccagcgc gacgatttat caaattcatt tttagtcaga actccctcag
     3961 tcgcgccaat atgctctacg tttgatgtag agtttgcagt agcaaatctt atcgccctct
     4021 cgatattcct agaatcaaac aatttcttgt ccccgccatc cattaatgct gcaaccatgc
     4081 ccgagccaaa actgtctccc gcacccgtcc tatcggctaa catcttctcc ttaaatatgc
     4141 cggcgctata tatgcgtctg ccgtcggagg caatcactcc ctccggacca agagtaataa
     4201 ccgcaattcc atttatcaaa ctggttaatt tctttaacat tctttctctt tgtttaaaag
     4261 gaattccggt taactcggat gcttcgccgt catttacaaa caaaacagaa acatatttta
     4321 aatcttggag taattttttc ttcccctcgc ttaaatgttt aaaacttgga ttaaccgcta
     4381 ttcttatgcc gcgttttgat gcctccctga ccaatttaga aaaaatagta taagagtcgc
     4441 cgggcagaga aacataaatc catttagctt ttaaattctt aaaatcaatg tcttttagag
     4501 aaaattcatt aatcgcgccg tgatagctta aaatagtgcg ttctcctttt tgcaaaagta
     4561 aaacagaacg agcagtataa gcctttagag agtgtttgat aaatttagtc tctattcttt
     4621 ctttttttaa gcgttttttt atttcctcgc cttcagcatc aaaaccaatt cttgccataa
     4681 ctgcggtctt aaaaccatga cgcgcgaaag ttacggctgc atttgccgca tttccaccca
     4741 atgtagcata ggcatcttca attccaaatt tttccccagt tggaattata tatgccttgc
     4801 cggtcggagt cttccaaggg gtagttttaa aatttattct gtaataagaa tcgtaagtcg
     4861 tagatcctat cgtaataata tcgtgcatga tacaagtata tagtatgagg tataaatact
     4921 gaatacttga tgtttaatgc tattttcttt ttataacttt attaacagcc ttgataatac
     4981 tcttcactcc cataccatat ttttctatca agtcctttgg cggaccggat tctccaaaaa
     5041 caccttgcaa accgataaat tcctgaggag ttggaacgtg ttttgctaaa atttcggcaa
     5101 ccgcgctacc cattccaccc gcaacctgat gctcttcaac agtcacaacc gctcctgttt
     5161 ttttagccaa ctcaatgatc tttttttcat ccatcggctt gatagtatga ttattcaaaa
     5221 caatcgtttt aataccctcg tcttccaatt ctttagccgc gattatcgca ttataaacta
     5281 gagggccaca tgcaattata acaacgtctg cttttttcct ctgcgacgaa gagtcccaca
     5341 taatatcagc tttgcctggg acaaaaggag atccatcagt cgtgataact ggcgtttttt
     5401 cacgcgcaaa gcgaagataa actggaccag caatctgggc agcggcaatg gtagcctttc
     5461 tagcctcaat agcatcgcaa ggaacaaaaa ctttcatgtt ggcgagaact ctcattgtcg
     5521 caatatcctc aaccgcctga tgagtagctc catccggacc gacggaaatt ccggcatgat
     5581 gtcctgcgat cttaacattt gaattattat aggagatggt cgtcctaatt tgttcccagt
     5641 ttctaccggg agaaaatgcc gcatacgaag agataaaagg aatcttgcct gaaattccca
     5701 agcccgcggc aatagtcgcc atattttgct ccgccactcc catctcaaaa aatctattag
     5761 gaaatttttg ggaaaaagct tcggacctag ttgattcagt caggtccgcg cacaaaacaa
     5821 caacattctc atccttctcg cctacgataa taagaccttc gccataaccg tttctgatag
     5881 ggacgttttc aacatcgtca gaaaatattt ttgggtttaa ttttgcaaat ttatttaaca
     5941 tgcttgataa ttttcaattt aaaatcttga atttccattc aattttaaat gttttaatat
     6001 ttgaaaattt attcattgga tattgattaa aaattggaaa ttgataattg taaatttatt
     6061 catgttctga ttctatctgg ccttctagag tcctcaactc ctttaatgcc ttatcggcct
     6121 cttctttctt gggcggcttt ccgtgccaca aaaaatctcc ctccatgaaa ctaactccct
     6181 tgccgggaat cgtgtgcgcg attataactg tgggtttttc ataaattgct ttagcctcat
     6241 tcaccgcatc tacaattgcc tccatattat gcccatcaat attcaaaaca tgccacccga
     6301 aagactcata tttatctcta agcggctcta aaggcattat gttttctgta tatccatcta
     6361 tctggatatt attcctatcg ataaccgccg ttagattatt taatttatat ttggacgcga
     6421 gcatcgctga ttcccaggtg tttccagagt tatgttctcc gtcagacatc aagcaataaa
     6481 ccctatattt cttattatta agttttgcgg caagagccat gccaaccgac tgccccagcc
     6541 cagaacccaa aggaccagat gtcgtctcaa ctccaggtaa tgcagtccta tgtggatgac
     6601 cttgcaatct ggaatttaac tttcttaaag tcttcagctc ttcaagcgga aaatatccag
     6661 catgagccat tgtcacatat ctcactgggc aaatatgacc gttggataaa atcagccggt
     6721 ctctgtcctc ccaatcagga ttttcaggat catgatccaa gatatgaaaa taaaaagcgg
     6781 tgaaaatatc cgccatgcct aaaggaccgg ctgagtgtcc gctaccggcc tcaagaagag
     6841 ttttaatgat atcctgtcta attatattag ccctctcttc taaaattttt attttctctt
     6901 cgtggattga atccatagtc taataatttg gtgtctaaat atcaggttgc caataattct
     6961 ttatacgctt gttcaggatt accgctctca aaaatgaaag aagcagaaac aactatatct
     7021 gcccccacct ctttgcacaa ccttgcggtt tctgagttta tacctccgtc tacttcaatt
     7081 ttaacattcg gcgctctctg ccgcaaaaat ttaatccgat acaaagagtc cggaataaat
     7141 ttctgtccgg ctaatcccgg ccttaccgca agtatttgaa aataatcaaa cttatccaaa
     7201 aatggaacag catcatcaat ctttacgtct tgatcaatag cgagcattaa ttctccgtta
     7261 tgttttttgg cggaatctat aacagactgc agattttcca gcgtttccaa gtgaacaatc
     7321 aatctttttg ctccaaccct aaaccaatcc tcagcaaaat catgcggatt ttttaccatt
     7381 agatgaatct caaaatttat atcaaataaa ctattatctc gtatgatcct tcccaactcc
     7441 tccggggtgc cccaagtaaa attaggagca aatttgccgt caactatatc aatatgaatc
     7501 cagcctcctt gacctataaa agaaacggca gttttaatct gttcttcagc cgtcttataa
     7561 tctccgcaat ttatcgccgg tattacaaac attgattaaa ttttaccata tttaagatta
     7621 actacttgca caacaaaaaa tggagccctt cacgcaagat gaatgactcc atggattaag
     7681 gatggattaa aaaggttaag acatcgtccc gagaaggacg ttttgacctt cgcgcatcga
     7741 agccatagcc cttgcgagcc caggatcatc gctctcaatg acgggaatcc cagagtggtc
     7801 agccagcatg ttgggctttt ccaaggtcgt aaccttgatc aacttcgggt tcacctcatg
     7861 aacagagacg atctcgcttt gcgcgagaac accaacgata agacgaagct tcggagcaga
     7921 gatcccagac ttttcgccga gatccttcaa gacagtcgtc tcgttcaccg acgggagaac
     7981 atcgatgacc gtctggagat cgcggcgggt tacagatgtg cgcaacccat cacgattctg
     8041 gacaaacggc ggcatcttac tccacacatg atcgctgacc tcaacgagct ggtctcccgg
     8101 atctcgcgag accaaagagt gcgatgcgcc attcgttccc tgcgcaggcg ccctcttact
     8161 agccaagcgc tgaagacact ctgtagtcgc gtcctcaata accggcggct tgtcaccgac
     8221 gaaagcctca cgagcctcga agaacgcctt aacggcggat ttcagactgc tcaactcatc
     8281 aggcgctatc ggctccttag gccttagccg aaccgcctcc ttaagaccca aatgcagcac
     8341 cctgatctcg gtaacccgac cctccttaac ttcccacgga agtacttcga agtctaccat
     8401 tgattttcct cctcaccccc atatgaggcg agttcattac agccgataaa aatagctatc
     8461 cgccaaaatg aactcacctc atatagatat attatttaaa gaacttccat actcacaaag
     8521 tatagatata aatatatcat tctgtcaaga agtccttaat tattcttttc aatctcgcca
     8581 attttatcca gcctcctctt atgcctctct tcttttgaaa atggagtttg aagccaaata
     8641 gcaatcatgc gtttcgcgtc ttcaggattt ataaaatcgg cagcaacaga caaaacattt
     8701 gtgtcgtcat ccacccttga aaccatcacc tgatctggag tatttgcgag cacggatcta
     8761 actctatcaa atttattagc cactatatca actccgacac ccgagccaca aaaaactaca
     8821 cctcgtcttc catcaggatc ggcggcaaca ttctccgcca cagcttttgc aaaatcagga
     8881 taatcatctg cgggatcgta tttgacattg cctacatcat gaatctgata accgttatct
     8941 ttaagataag tcttaataaa ttctttcagt ttaaaccccc tatgatctga tcctaaataa
     9001 atcagcatta tccccagata gatataacta tcgccatagt cataagagat gctagatgat
     9061 aaccggcatt caaaacgaaa agcgccatcg gcttcggcgt tggagagaaa agatattcgg
     9121 aaatattagt tgtcgccaca aatccgatcc atacccagaa agcgatttga atcgccgatg
     9181 aaatgctgac cggcggagcg tttattataa aaagagataa aacataagcc ataattatgg
     9241 ccgcaaaaaa agacagaaaa taagacttca tcgctccctt aggtttcgcg tcaaccgaca
     9301 cgccaaccaa actcatccac ttcttaccga aacctattgg tgaataccaa atcatcccta
     9361 aggccatact tgctatcgcg gctagaataa ctacaacgaa atttatatct aatgtataca
     9421 ttgaaagttt ttaaactata acgactttct gaacatgctt aaccaaagtt gaaacatatc
     9481 catattcatt atcataccag gaaaggacat ttaccaaatc gccgtcaatt accttggtaa
     9541 actttaaatc tataatagcg gcgtgagtat tccctataat atcagaggaa actacctgat
     9601 cttcagtcac atatagaatc ccatcccaac gaggatcttt agcggcattc ttaaaaattt
     9661 tatttatctc ttcaaccgtt gtcttccgtt tagcgagaaa tgtaatattc gccgccgaac
     9721 cggtaatggt gggtgttcta aaagatattc catcaaattt tccttccaag tcaggtataa
     9781 ctcttgtgat cgcaatagcc gcgccagtgg ttgaaggcgc aatattctgc gccccggccc
     9841 tacctcttct gtaatccgat cctctgactg gtccgtcaac tatattttga gttgcagtat
     9901 aagcatggac tgtattcaat agcgctttct gtatgccaat cgtttcattc attatagaaa
     9961 caactggtga agtagcattc gtagtacaag atccatttga attaatagag caaacgttta
    10021 tttctgaatc attcgcaccg agtaaaaccg tttttccaag ttctccgtca tcatcttttg
    10081 ccggagcgct tataacaacg cgtttcgcgc cggcgtctaa gtgcattttt gctttttcgt
    10141 aagactcaaa aactccagtt gattcgacga ccacatcaat attcaaatct ttccaaggaa
    10201 gagtagtagg atccttaatt tgtagaaatt ttatgtcttt accgttcaca accaaaccgc
    10261 cgtctctcac ctcaactgat ttatcgtatt tcctataaac ggaatcgtat tgcagaagat
    10321 aacttaaatt atgcgcatct cctaaatcat taattgcaac tatctctatg tccgacagac
    10381 caaaagcctg cctgaagaaa agcctaccaa tccttccaaa accatttatt gcgactcgag
    10441 tcatgcagta attctataat attttgtttg caatacaaaa tattatatag aattaccaac
    10501 actcgcctga attctgcaac gcagaactta gaccgatttg gcatagggtc tttcatcccc
    10561 cttttatccc catggggata aaagggggat gaaagattaa ttaatctttc gtaaatccac
    10621 ttaagttagt gcaaaaatcc tatatcttgt gtctgcgatt tataagaaga agcatgacga
    10681 gaccagatgc agaaacggca acaacataac taagcgcgac tgcatgacca aaagaaaacc
    10741 ataaaagtcc gcctaccgtg ccggcaaata acgaagacaa acctatggaa gcttgcaaaa
    10801 tcccctctcc ggctcccaac aactcatgcc ccattaattt agcggcgaga gctctttgta
    10861 tgccatcggt cgcgccagag taaactccaa gcgccataag agcgaggatc aaaccccaaa
    10921 gagatgcggt taaaatcaaa ataatatatg cagtgatggc taagataaaa ccaataacta
    10981 tgactttaac cggaccaact ctatctgaaa gtctgccaag cggcgcagaa cttaaagtaa
    11041 aaactaaacc ataaataaaa tataagagcg gaacaaattg gtcgcccaga cccagctcac
    11101 caaccctgaa aagtatcaat cctatcggca atattccaag tccaaaaaca aataccgcta
    11161 taataaataa ggtgaagtgc ttgtgagtct taaataaatt ttcttttaag tgtttattat
    11221 tcgatatcat tccagcgcct cttatctctc gcacccagaa gaagctgccc aaagcaagaa
    11281 gtccaataat aaaagaaatt ataaaaaggg ttggaaaatt attattaatc aatggaagta
    11341 ttgcgagcgc agctaaaggg ccaagcaaac cgcccaaagc atccatagaa cgctgatacc
    11401 cgaaagattt gcctaaatcc gcatccttaa cagaagaggc aagtaacgcg tctcgtggag
    11461 cgtcgcgcaa tcccttgccg aacctatcca gcaaacgcaa agaaaaaaca tggctgaaag
    11521 ctgaagccaa accaagaaac ggcctagtaa caaccgacaa actgtatccc aagatagcaa
    11581 atggttttct tctgccgact ctatcagaaa ccactcctga tgcgacctta acaaaactgg
    11641 acaacgcatc cgctaccccc tctataatcc caagagccaa aaccgcggtg ttacccaaaa
    11701 tacttgtaag gaatagcggc atcacagatt ggatcatttc atttgagaaa tcattaaaga
    11761 gacttaccaa tcccaaaaag aaaacgttct tccgcagacc gaatattttt ttatcagatt
    11821 tatccactat ttatttattc tatagatgaa ttgttatttt atcaccaatc tcaatctcca
    11881 aaaaatctat tacgccgggt ttcaattcta aaactttatc aaccggctct ttggatcgat
    11941 gaagcctgat ttgatttaca agtccagtat gcggaactaa tttttcaaat cctaccacct
    12001 catcgttatc aatccataaa atatccaaat caaatctcat gccaaacatc caaaacgggc
    12061 ggcgcgcctt attcttaaaa gcgaacagca tgccatctgc tccgtcgcga acatcttttt
    12121 gccatgcaag accgatagct ctctttatga aattgtcagc tgtagtcaat ttaaaatcgc
    12181 tacccttaat atttgcaacg caaaatttgt ccatctctat tctttaaatc caagcttaac
    12241 aacaattttg tgccgtctgc catcatttag aatattaata gtgtttgatt tattatcccg
    12301 cccatctatt ttcaccgata ccacaccctt attcacgcca tttggattat aaatagtaat
    12361 cagatattcg gtattgccca acttatacga aatagagcat ttatcccaat ccttaggaag
    12421 acacgggtct atcgtcatag tatcttttga gatattcaat ccgaaaataa attccaatat
    12481 cgttctataa aataatcctg ctgatcctgt ataccaagtc caccctccgc gtccatcata
    12541 agaaggcgga gcataaacct ctgaagacag aacgtacggc tcaactcgat aacgcgcaac
    12601 atctttctga cttcttagcg ccggatttat aaggtctaga atcttctttc ccgaatctcc
    12661 atcaccaaga acaaagagtg cctgagcaag ccataacgcg gcatgattgt attgtcctcc
    12721 gttttctcgc actccaggcg gataatcttt aatagatccg ggatcaaaaa ccacactttg
    12781 caaaggcgga gccaaaagcc ttaccacatc tccgtcaacc aaattaccca aagcagattc
    12841 tatagctctt tttgctcttt cattcttccc tccaccactc aacaccgacc acgactgggc
    12901 tatggaatca attttaaatt cttggttttg agaagaacct aaaggaatcc tattatccat
    12961 aaacgctctc ttataccaag agccgtccca cgcttcttct tccaaagatc tttgcaagcc
    13021 tgaggccgca gccctaaagc gttcagtatt tttataatcc ccacgcttct cgctaagatt
    13081 agaaaatctc ataagggtag cgtataaaaa ccagccaagc caaacgcttt cacctttacc
    13141 ctctactcct actttattca acgaatcatt ccaatcgctg actccgaata aaggtaagcc
    13201 gttcggacca aatttcattg tgtgatttat agccctaatg cagtgttcat aaatagaata
    13261 cctatcggtt tttgcatcag gcacgccgac ccactcaagt ctgtctataa aattaacgcg
    13321 cggtccatcc aaaaattctt ctctctcctc taagacagac tcgtctccgg tgatattgat
    13381 atatgaagtt attacataag gcagccataa ttgatgatct gaaaaagccg tacgtattcc
    13441 aaaattatta tgatcgtgcc accaattctg cgcgtcaccc tctttaaatt gatgcttagc
    13501 cgcttttaga atcaactgcc gaacaagcgc cggttcccta tatataagag ccattgaatc
    13561 ttgaagttga tctctaaaac catatgcgcc ggaagactga taatacccgg tcttgcccat
    13621 tatcctgctt gataaaatct ggtaaagaag ccagtcgtta aaaagcatat taagagattt
    13681 gtcgggacaa tctattttaa aaggtccatt ctcccatttt gctatcgcgc gcgaaagaga
    13741 ggttgctgac cttattttgc ttctatattt agaaagataa tttttaacct cgtctttagt
    13801 tgttccctca ccgataagcg ctattatttc cttttcttcg ccgggcgcga ggtcaacacc
    13861 cgcttgtaaa gcaatacaat tatcaataat gttctctcct aaatcttctt gtcctttatc
    13921 tattctttct ctatgaagcg cagatggata atttattgat ccgtgacgcc ctaaaaaatg
    13981 ttcacggttg aaactataaa caaagtttcc acctgccata tcaaaaaaag caatcctgtc
    14041 ccttaaatgc gacctaaatt tattctcaac caatacagcc ttaacagatg aattatattt
    14101 tatatctaaa acgtgcctag tatcctcctt gtttgatcct aaaacaggct cgaataaacc
    14161 aacaagagaa agtttttttg atttagaact taaatttttt aatttaacat tcactatctt
    14221 aaccggatcg tctaccggaa cataggaaag aaatttatgc tctattccgt ttctattatg
    14281 atcaaagctt gaaaatcctc ggccatgcgt tactcgatat agatgtctgc catacgaagg
    14341 ataaggagta ggcgaccaaa cttcaccggt ctcatcatct ttaatataaa atatttctcc
    14401 actgccatat ctatactcat ctctgttaag agcagttaat cgattgtcat agctgtcttc
    14461 cgaccaggta aagcttgatc cattttctcg catcaagaag ccgaatttgt catttgttac
    14521 gatatttgcc catgacgaag gagtattgtc atccgcgcct gtcagaaaaa cataagaacc
    14581 gtctttagaa tcaaagccgc cgaaaccgtt gaaaagctgc gtctcaacat ctagagatat
    14641 gtttggcttg tctaatgacg gtttgattcc agatcctaat ctgtcgccaa aaattgtcct
    14701 accatagcca ttgcgtaccg agatttgatc agagagggcg ccagacttgc tgtctaaaat
    14761 aaatcttgat gcaaaaagta aaatctcttt atcaatatct gatactaaat ccgatttaag
    14821 atgaaaaacg cctccccctc cagaatcctc tctggctgct cggtttgaac gaatcataaa
    14881 atctatttca tcatcaagcg tccttatata acttgaagga tgctcattca aaaccacgat
    14941 atcgacatcc agtcctttat atctcatata ttgattgaat aataatatct gcttaacaaa
    15001 aggagcatcc tcaatgtctt taactctgac tgacaaaatt gggaagtctc ctgaaatgcc
    15061 atgcctccac aaagattcaa taatagattt tctttctctc gcgacagaag gcataggctt
    15121 aatttcacca accattatcc tagaagctag agattggaac aaaatcgcat cttctgcaga
    15181 aatatttata tccttcaatg tttttatgct taaattatta ttttcatgaa taactttccg
    15241 cgatgaataa ttctttgttt gttttttaat aaggcgcatt atctcctctt tagcgctgcc
    15301 gacgctattg ataaaaacta tcttggctgt atctcctggt tttatggata tttttgcatt
    15361 taaactaaag gttgaatcaa gggtgtgttt aggttctaaa tcttttttga ttttattatt
    15421 atgtataaat gcgggcttgc taattgaacc gtttcttccc aaaaaatcct ctctggatct
    15481 cgcatagccc attaactcag cgtggcgagg caaaataacc gaatgagcaa aatatatttt
    15541 tcttgatcta tctgaaggat gccttctatt gaaaaggagg gtgcgatgcc tcgcaatgaa
    15601 ctcagattca accataagct tatgaaacat cggatgatcc ttatcggctc ccggttttgt
    15661 catgaccacc tcgccaaaac tcgccacctc aatatttcta actacgtctc cggcgtttgt
    15721 aagagacagc tctcttattt ccatattttc aacggatggg ataaaaaccc ttaaattact
    15781 ttctatattg ttataaaatt gtctaaattc aactcggttt tcatgaaaaa taattttatt
    15841 tttctttcca ccttttctca ttggttgaaa agtcggcgac caaagctcac tggtatccaa
    15901 atctttcata tagataaatg agccccactc ctccaacacg ggatcgtctt taaaacgtgt
    15961 aagctgtatg tcttgatact tgctgtaacc ggcaccgcta ttactgaccg ctaaaaaata
    16021 atttccgtta gacaagaggg cgtatctcgg gattcttgtt tttataggaa tgtacttgct
    16081 aatgagattg gtgtcaggag ttttcttttt aggaacgatg ctcgcgcttt taactggacg
    16141 gagaggaatg gtcctgggag ctccctcctc cagaagagca gaaatagatt caaccctcgg
    16201 atccatgtga aagagtttta tcatccttcc gtcctgaatt aaattactta taccagccag
    16261 ggtaaatccc tggtggtgcg cataataaat ttttaccgga atgccagagg cgggtttaaa
    16321 tcccgttgta ccggtataat caaaggcatc ataaaaacca taaatcccca aaactccctt
    16381 cctgcccaac cttttaaaat tccttatggc tattttagga taaaaacgca aagcgagcgc
    16441 tgtagtataa ggagcaacaa cgcgataatc ttttaaccct ctttttaaac ccagtatagg
    16501 aacgccgaaa acctggtact gatagctctg ggacgcatca aacatattat aagccgactc
    16561 tcctatgccc cacggcatcg cgaacaaccg agcataattt ctatgagttt taatagcagt
    16621 ccttgcagtc tctcctatca aactctccgg gtgcacgttt aaaaaaagca gggatgttaa
    16681 atattcaaaa agcgaaccgc cccaagaaag catcacaccc ttattgccaa ccctaacaag
    16741 cttacgcccc agatggaacc aatgcttttg cggaacatct cctttcaata ttcctatgaa
    16801 actaataagg ttagcttcag atgccataaa atcataataa gcgttgtcta ttttgttaaa
    16861 actagcattg tatccgatat gaaaaagtcc gcgctcctta ttataaagaa atttaaattc
    16921 ggttccgtta aaaattttta acgcgttttc agatatgttg ctcgcttctt taataaggct
    16981 aagcgcgtag gccttggatt tctttatctc atcacggatg ttttgaatcc acgatctgag
    17041 caaaacgatt tcttctttgc tcactcctgc gctagataga gaatctaacc taacagaatc
    17101 taattctatt tctaaacgat ctgcctcaaa atcgcctata ctgggaacct tatctataat
    17161 ttttgcaatt aatgcgtaaa aatctctcaa ctctacaatc tctttgcgta caggaatatt
    17221 tgaaaaaact ttataaaaag aagctccgga ctgaaaagtc ctataacact cttcaaccaa
    17281 ctcctcaaga aatttaaggc tcttttctat gcccacaaaa aattcttctc ctccagtaaa
    17341 ttccaagccg gcaagccttg atcttgtatc gctattaaaa agagctatct catttaatat
    17401 atttaaaaat cctgatagtt tttttgtatt tcgcacagat attgatctaa taatcttgtt
    17461 taaattatta gatatctttt ttatatattt tttagcgcct tgagcggctg acggcgacct
    17521 gaataaggta tgtaaatctt ctctcaaaac acctaaagca tccaaaaatc ctgcttgcaa
    17581 attattacct agaattttat cccttctaag ttcttttaag gacccttgca aaaccaacat
    17641 attcgctaac atattggcgc tatcgacgct tgatatatat ttaggaaaaa gtggttttaa
    17701 agttttaaca tcataccaat tataaaaatg tcccttgaat ctctctaatg aggatagtgt
    17761 atgaaaagtc ttagtcaggt tgttaataaa cctttcaaag gatatgtaac ccaattcata
    17821 tccgatcatc aaagaattta tcagcgcgcc tagattcgtc ggagaagttg taactttata
    17881 ttccgttctt gcaactctgg gatattcctg cagatgatcg ggtatcaacc aattgttcga
    17941 ttgatttgaa aattctataa agtatctaaa attgcgatac gcaattcctc ttaaaaaatt
    18001 aatatcgctt tctctcggtt tatatttttt atgtcttgga agagaaagaa tgtaagcgat
    18061 gaacggcgac aaaaaccaca aagaaagcca aacgtaagcg gctactccgc caaaacccag
    18121 aaaatataga ataagagaca tcgcgccaga aaaaaaagga gtaagccaca ttgatttata
    18181 aaatctaatc aatcctcctc tgttttcctt tgctgcctct tgtgaggcgg tccactgtaa
    18241 aagtttcttt ttagtgataa agagtcttga tacggctctt gctattgcgt ccacataaac
    18301 aatcgactgt tggaataaaa gagatagtcg ggtgaatatc tgcagaataa caacaaaaga
    18361 tttataaaca accattttaa tggcgtctat aaatggaaat gtcctaaaaa aactgaaaga
    18421 ctcagcaaaa aaaactataa cgaatggcga tcccaaaata atgattggat aaatatccca
    18481 aacgttatgc aatggagaaa ataaaataaa gaatattatg actaaagaaa aaggggggat
    18541 aaggctcctg cgtaaattat caagaattct ccatttatca tcaacaccaa acctattccc
    18601 gcgcatacca tcttttactc gtaaaaagtt tggtctcaac cagtgaatta tttgccaatc
    18661 cccacgtatc caacggtgca gtctagtcag atattcatta taattttgag ggaagccgtc
    18721 aaaaacctga atatcgctcg ataagccagt cttggcatat aatccctcta acaaatcgtg
    18781 gcttaaaacc ctattttcag gtatattatc tttcatagtc tcttcaacaa cctcaacgtc
    18841 ataaattcct ttcccaaaaa atattgcatt cccgaataaa tcctgataca catcggggct
    18901 cggccttgaa taagaatcaa gaccaataga aaaagcaaaa gcattagaaa ataaagagta
    18961 tttagcagag tctattctgg tcgcaatctg aggctggatt atcccataac cccgcgaaac
    19021 ccttccttca tcacgcttca gaattggttt attaagagga tggctgatgc atccaatcat
    19081 tttttttata gtatcctgcg gaacttctgt gtcgtcatct aaagtaacca catatctgac
    19141 cgatggaacg tttaatccat ttttaggaat agcgtatgtg acatcctcgt ctcctaaaat
    19201 cagcttatta aattctctta gcgctcctct cttcctctcc caacccataa aaactttatc
    19261 cttttcatta aaaatcctat gcctatacaa agagaagaat ctatctcggc ttcctgcaga
    19321 cttatatttt ttattcagcg cgtctatacc attttctaaa aatttaatat attcagagtc
    19381 ttctttatta atctcattct tagaatccct aaagtccatc aacaagccaa agaaaatatt
    19441 tttatctcta ttgcccaaat aatggacctc gagctttctc aataaattgc cgataagaat
    19501 tttactccta aaaactgatg gaataacgac aagagtcgcg tctttatccg gaatatcatt
    19561 cactacataa tctatcttcg gcaataaacg caccggcgtt aatctagaca gaacagaatt
    19621 gactaaattg accgctatat cgctgttcag aataaaaata attatggaaa cgggtaatag
    19681 cgcccatgct ccaacatata tccccatcag agagataacc gcataagtga aaattaaagt
    19741 aagaaaaaaa atgggtccga gatatattaa cccgggatgc ttaagagcga atctataaat
    19801 acgatctgaa aaagaaggtt tatatccaat tttttgttct aacacagtcc tgccatcatc
    19861 aataataaaa tatcctacat gctcca
//
LOCUS       DATDNZ010000065         8027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_712040, whole genome shotgun sequence.
ACCESSION   DATDNZ010000065 DATDNZ010000000
VERSION     DATDNZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712040"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VI432_02485"
     CDS             complement(<1..544)
                     /locus_tag="VI432_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY4496991.1"
                     /translation="MSHNLAKVFSAELEGIEAKLIDVEVDLNTGLHSFNIVGLADKAL
                     SEAKERVSSAIKNTGLKPPNKENRKITVNLAPADIKKTGSQYDLAIAIGYLSATKQIK
                     AFEAENKIFLGELSLNGDLRPVSGALNYAILGKTIGAKYLFVPNKNAKEASLIDGINI
                     IPVKNLGDLISHLNEIKYIDA"
     gene            complement(544..873)
                     /locus_tag="VI432_02490"
     CDS             complement(544..873)
                     /locus_tag="VI432_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496992.1"
                     /translation="MLNKSEIEKFKNILLDEEKILQNKIKEMEAPTDFGDAVDHGEEE
                     SDEDEELENRSSVAETYRDRLVNVQSALNRITENKYGLCSNCGNEIETDLLNSVPESN
                     LCKDCKK"
     gene            983..1249
                     /locus_tag="VI432_02495"
     CDS             983..1249
                     /locus_tag="VI432_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496993.1"
                     /translation="MAGKQTQRPLVSASILALSSVVVLGGIAALVVWNIGNGEGPVFK
                     AYANGVLMLTGVVLVILVALLAATLKQNYLLEEILERLSGKKRS"
     gene            1311..1886
                     /gene="ruvA"
                     /locus_tag="VI432_02500"
     CDS             1311..1886
                     /gene="ruvA"
                     /locus_tag="VI432_02500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY4496994.1"
                     /translation="MISSLKGKLSDKKANYIIIDVGGVGFKVFISLANQGVLPSIGSM
                     VEVLTYLHVREDGLTLYGFLNEGEIDFFESLISVSGIGPKSALGIMSVAPIEKLSAAI
                     SEGQIELLQRSSGVGRKTAERIIVELKDKIKLEGSGRTVEMMESDKDIYDALLSLGYS
                     AKQAKDAVSKVNPEIIDSSERLREAFKIIKS"
     gene            1887..3107
                     /locus_tag="VI432_02505"
     CDS             1887..3107
                     /locus_tag="VI432_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY4496995.1"
                     /translation="MVKSIIKFLKGRYHLMLSYLGRVLYMNPSKSIFVVGVTGTKGKT
                     TVIEIINFILESAGFETAISSSLRIKIKDQSGPNKQNTMPGRLFLHKFLRKAVDAGAD
                     FALLEVTSEGVAQHRHRNIHFNSAILTNLQPEHIESHGSFEKYREAKLKFFRDVARDE
                     AKPDKVFIINKDDPNYKYFSDAVLENKTTFYSKSNLESNMPGEFNKYNIGAAAAFLKE
                     VGVDELKIEKGLKDFIGVSGRMELVVEKPFKVIVDYAHTPDSLEAVYKTLSSEVKIRE
                     NKLVCVLGSAGGGRDKWKRPKMGEIAALYCKEIILTNEDPYDEDPDKIINEIEAGILG
                     SHKPNFETNKNYWRFMDRREAIEKAMSLAKKNDIVIITGKGSEPHIHLAHGATMEWSD
                     KKVVMEVLKGRKSQ"
     gene            3198..3404
                     /locus_tag="VI432_02510"
     CDS             3198..3404
                     /locus_tag="VI432_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496996.1"
                     /translation="MLKDWNHDLIQQLSEISDSLWRMEDYKKVSKGCEHCSMLWDKLE
                     KDYEAHIKMLTGEITRHVKEERFN"
     gene            3432..4799
                     /locus_tag="VI432_02515"
     CDS             3432..4799
                     /locus_tag="VI432_02515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4496997.1"
                     /translation="MKIKFFGGTEAVTGSNFLVEIKGPRGSESQILIDCGLHQGSNFC
                     EKHNFEPFPYDPQKIKAVFVTHAHIDHTGLLPKLYRQGFKGKVYSTPPTKDFAYELLL
                     DSENILFREAERENKEPLYSKKDIDGLMGVWEGIDYGTPIEVGGFSVTLRNSGHILGS
                     SSILVKADDRLVVFSGDLGNYRPPIIKESEVLDIPADYCLIESTYGNRLHEGLELRKG
                     ILENVIEETVKTKGVLMIPSFAMERTQEILYELNELVENGRIPRVPIFIDSPLAIKLT
                     RVYQKYSYYFNDEASRLVKKGDALFFFPGLKMTLNKDESIAINDVPSPKVIIAGSGMS
                     QGGRILHHEKRYLSDPNSAILFIGYQAYGSLGRQILEGAKSVKILGEDIAVRCRVRAI
                     GAYSAHADKRKLLEWLYPMRSTLKKVFVVQGEKEASEALASAIKDKLAVDARAPSIKE
                     EVVLE"
     gene            4805..5398
                     /locus_tag="VI432_02520"
     CDS             4805..5398
                     /locus_tag="VI432_02520"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4496998.1"
                     /translation="MVNGENLDLKYKICVSGAAETAHCGQKAFEIAEELGREIVRQGA
                     VLVDGATTGFPYWTAKGAKAEGGTVVGLSPAVSEKEHVETYKLPLDFHDLVVYTGFNY
                     SGRNLLLTRAADAVVVGCGRLGTINEFTIAFEDKKPIGILEGDWETDEVVRNIIDKSH
                     RADEVRDRIVFDSDPKSLISKIIEVIKLDKASNKMVL"
     gene            5452..6570
                     /locus_tag="VI432_02525"
     CDS             5452..6570
                     /locus_tag="VI432_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HEY4496999.1"
                     /translation="MDKILSWYREFLLPAVLLSALIIGAGMFALPYLFDQAGFISGLF
                     WLALLTVVVSTVHLMYLDVTNYTEGRHRFVGYARIYLGNWGFFISFITTALGNILGLL
                     IYIILAVSLIKVILPNLNDFIALIIFWLFSTAAFVAGIRRMANIEFWGTVGTVIVVFA
                     IFLGGVGGFDAGAVKLIDFNNFLIPYGAILFSLGGRAAISSIKTYISENNLSSKHLNK
                     AVIVGTVIPAILYLLFIIGIIGLSGGSVSENAVAGLYTISPLLIYTIALLGVFELWTS
                     YIFLGLEAKEILSYDLSVSRFFASAIVMFLPIVLYIIGFNNFIVAVGIAGGIFIAIEG
                     IFVISMWLRLRSRSGLALNMARIMVVMFILGGLQELLY"
     gene            6650..7096
                     /locus_tag="VI432_02530"
     CDS             6650..7096
                     /locus_tag="VI432_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497000.1"
                     /translation="MNNGGALMKRNQIKTFDYRRGFGFTMNGVFVHMNDGRVPELVGD
                     KVVLGSSVMPPDRYPKQGDRIVFVIKRIDGKRNAAPWCFEDDYERIKNKLRESIVSGL
                     ISKLGDQGFPPGEMSWGDGSINVRISIRRSPKGPSNRRKVLSFAVG"
     gene            7189..>8027
                     /locus_tag="VI432_02535"
     CDS             7189..>8027
                     /locus_tag="VI432_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY4497001.1"
                     /translation="MTLFGEITIALILASALGIFFYLLRQPTIIGFILAGIAISYFGY
                     LSTEGRFEIINVLSSIGVALLLFIVGLEMSFKELKDVGKQSIIIAFAQAIITFSVGYF
                     IATLLGFSVIVSIYIGIALTFSSTIILVKLISEKNELKSLYGRIALGSSLIQDLMAVI
                     ILILLAGLSDGVSALTSASAVIKGLFFVFLAILSSKILPRFLDFIGTSPELLYLFSLS
                     WAIGWAAFLSSPIIGLSIEIGGFLAGLSLANSSEHFQIGARLKPLRDFFLIIFFIGLG
                     AKML"
BASE COUNT         2331 a         1248 c         1781 g         2667 t
ORIGIN      
        1 gcgcgtctat atatttaatt tcatttagat ggctgataag gtcgcccaaa tttttcaccg
       61 gtattatgtt tatgccgtct attaagcttg cttctttcgc gtttttattc ggcacaaata
      121 gatattttgc tccaatagtc tttcctaaaa tagcgtaatt cagcgcgccg gataccggac
      181 gcaagtcgcc atttaaagac agctctccca agaatatttt attttctgct tcaaaagctt
      241 ttatttgttt tgtggctgat aaataaccga ttgcgattgc aagatcgtat tgcgagccgg
      301 tttttttaat atctgccgga gccaggttaa ctgttatttt tctgttttct ttattgggag
      361 gctttagtcc agtatttttt atagccgagc tcactctctc tttggcttct gacaaggctt
      421 tgtcggcgag gccaacaata ttaaaactat gcaagccggt gtttaagtct acctctacgt
      481 ctatcagttt agcctctatt ccttctagtt cagcggagaa tactttggct aaattatggc
      541 tcattatttt ttacagtctt tacacaaatt tgattcgggg acggaattta ataaatctgt
      601 ttctatctcg tttccgcagt ttgaacacaa tccatattta ttctcagtta ttctatttaa
      661 tgcagattgc acattgacca atctatctct gtaggtctct gcgacgcttg atctattttc
      721 caattcctcg tcttcatcag attcttcttc gccgtgatct accgcgtctc caaaatcagt
      781 aggagcctcc atttctttaa ttttattctg taaaattttt tcttcatcaa gaagaatatt
      841 tttaaatttt tctatttctg atttgtttag catatctaaa agttatctta ccgccttttt
      901 aaagtcaatt gccgcttgat atagtcggtc tgccattcta agctgttaac agtcctactt
      961 aaaatatcaa agaggcgcta aaatggctgg aaaacaaact caacgaccat tagtcagcgc
     1021 atctattctt gctctttcaa gcgttgtggt tttgggaggg atagctgctc ttgttgtttg
     1081 gaatatcggc aacggagagg gtccggtctt taaagcttat gcaaatggag ttttgatgct
     1141 gaccggcgtt gtcttagtaa ttcttgtggc attgcttgcc gcgaccctaa aacagaatta
     1201 ccttcttgaa gagatattgg agcgcctgtc tggcaaaaaa agatcataag accaagttgt
     1261 tttacttggt ctttttcagt agctggctat ttttataata taatgtattt atgataagca
     1321 gtttaaaagg aaagctatct gataaaaaag cgaattatat tattattgat gtgggcggag
     1381 ttggttttaa ggtttttatt tcgctagcca atcagggtgt tttgccgtct atagggtcaa
     1441 tggttgaggt tctgacttat ctgcatgttc gagaagacgg tctaacgctc tacggttttt
     1501 taaatgaagg agaaatcgat ttttttgaaa gcttaatttc ggtttcgggt ataggaccta
     1561 aatcggcttt agggataatg tctgttgcgc cgatcgaaaa gctttctgcc gcgatttcag
     1621 agggccagat tgaattattg cagagatctt ccggcgtagg aaggaaaaca gcagagagga
     1681 taatagttga actgaaagat aagattaaac ttgagggatc tggcaggaca gttgagatga
     1741 tggaatccga taaagatatt tacgatgcat tgttgagttt gggttatagc gcaaaacaag
     1801 caaaagacgc tgtttcaaaa gttaatccag agattattga ttcaagcgaa cggcttagag
     1861 aagcttttaa aataattaaa tcttagatgg tgaaatctat aatcaaattt ttaaaaggaa
     1921 gatatcactt gatgctttct tatctcggca gagttttgta tatgaatcct tcaaagagta
     1981 tttttgtggt tggtgtaacc ggcacaaaag gcaaaactac tgtaattgaa attataaatt
     2041 ttattcttga gtccgccggt tttgagacgg ctatcagctc ttctttaaga ataaaaataa
     2101 aagatcaatc cggacctaat aaacagaata ctatgccagg aaggttgttc ctgcataaat
     2161 ttttaaggaa agcagtagac gcaggagcgg attttgctct tttagaagtt acttcagagg
     2221 gggtagcgca gcataggcat agaaatatcc attttaattc ggccattctg acaaatttgc
     2281 agccagaaca tattgaatcg cacggaagtt ttgaaaaata tagggaagcg aaattgaagt
     2341 tttttagaga tgtcgcaaga gatgaagcaa aaccggataa agtctttata attaataaag
     2401 atgaccctaa ttataaatat ttttcagatg cggtactgga aaacaaaaca actttttatt
     2461 ctaaaagcaa tttagagtct aatatgcctg gtgagtttaa taagtataat atcggcgcag
     2521 cagcagcttt tttaaaagag gtaggagttg atgaattaaa gatagagaaa ggtttaaagg
     2581 attttattgg agtttctggc aggatggaat tggttgtcga aaaaccattt aaagtgatcg
     2641 ttgattatgc tcatacgcct gattctttgg aggctgttta taagacttta agtagtgagg
     2701 taaaaataag agaaaataaa cttgtgtgcg ttttagggtc ggctggtggg ggcagggata
     2761 agtggaagag acctaaaatg ggtgagatcg cggctttata ttgcaaagag attattttaa
     2821 ccaatgaaga tccttatgac gaagatcccg ataaaataat taatgaaatt gaggctggta
     2881 ttttaggctc gcataagccg aatttcgaaa caaacaaaaa ttattggaga tttatggata
     2941 gaagagaggc gatagagaaa gctatgagct tggctaaaaa aaatgatata gttattataa
     3001 caggcaaggg cagtgaacct catattcatc ttgcgcatgg agcgacgatg gaatggtctg
     3061 ataaaaaagt tgttatggaa gtattaaaag gtcgtaagtc acaataatac aaacatacta
     3121 ataatacgga tagaagaatt ataattagta tttattcgta ttattcgcat gcatttgtaa
     3181 attagtatca cagatttatg ttaaaagatt ggaatcatga tttaatccaa cagttatcag
     3241 aaatatctga tagtctttgg cgcatggaag attataaaaa agtatctaag ggttgcgagc
     3301 attgctcaat gctctgggat aaacttgaga aagattacga agcccatatt aagatgctta
     3361 caggagagat aacacgccac gttaaagagg agagatttaa ttagagttta aattaattag
     3421 atgatattca tatgaaaatc aagtttttcg gtggaactga ggctgtcaca ggttcaaatt
     3481 ttttggttga gataaaaggt ccgagaggat ccgaatccca aattttaata gactgcggtt
     3541 tgcatcaagg aagcaatttt tgtgagaagc acaattttga gcctttcccg tatgatcccc
     3601 aaaaaataaa agctgttttt gttacgcatg ctcatataga tcacacgggc cttttgccta
     3661 aattgtacag acaaggattt aaaggcaaag tttattcaac tcctccaaca aaagattttg
     3721 cttacgagct cctattagat tcagaaaata ttctttttcg tgaagcagaa cgagaaaata
     3781 aagagcccct ttattccaag aaagatatag atggattgat gggtgtttgg gaggggattg
     3841 attatggtac gccgattgag gttggcggtt tttcagtcac gcttagaaat tcgggacata
     3901 ttttaggatc cagttctatt ttggttaaag cagatgatcg actcgttgtt ttctctggcg
     3961 atttaggcaa ttataggcca ccaattatta aggagtcaga ggttttagat ataccggctg
     4021 actattgtct tatcgagtca acttatggga atagacttca tgagggtcta gaacttagaa
     4081 agggtatttt ggagaatgtg attgaagaaa ctgttaaaac taaaggagtt ttaatgatcc
     4141 cttcttttgc tatggaacgc actcaagaga ttttatacga gcttaatgag ctggttgaaa
     4201 atgggagaat tccaagagtg cctatattta ttgacagtcc gcttgcgata aaacttactc
     4261 gagtctatca aaaatatagt tattatttta atgatgaagc ttcaagatta gttaaaaaag
     4321 gagatgctct atttttcttc ccgggactta agatgacttt aaataaagac gaatctattg
     4381 cgattaatga cgttccctcg ccgaaggtga ttatcgcggg ttccggtatg tcgcagggag
     4441 gaaggatttt gcatcatgaa aaacgttatc ttagcgaccc gaacagcgcg attttattta
     4501 ttggctatca ggcatatggg tctctgggta ggcagatatt agagggggca aagtcggtta
     4561 aaatattagg cgaagatata gctgtgcgtt gcagggtaag agcaatcggc gcttattcgg
     4621 ctcatgccga taaaagaaaa ttgcttgagt ggctttatcc tatgcggtca acgttaaaaa
     4681 aggttttcgt cgtgcaagga gagaaagagg caagcgaagc tttagcatca gcaattaaag
     4741 ataagctggc ggttgatgcg cgagcccctt caataaaaga agaggttgta ttagaataga
     4801 gtacatggtt aacggagaaa atttggattt gaaatataaa atttgtgttt ctggcgccgc
     4861 tgaaactgcg cattgcggac agaaagcttt tgaaattgca gaggagttgg gtagggaaat
     4921 agtgcgtcag ggagcggttt tggttgatgg cgcaacaaca ggtttccctt attggactgc
     4981 caagggcgca aaagctgaag gaggcaccgt tgtcggtctt tcaccggctg ttagcgaaaa
     5041 agaacacgtt gagacgtata agctcccgtt agatttccat gatttggttg tttatactgg
     5101 atttaattat tcgggcagga atttactctt aacaagagcc gcggacgcgg ttgttgttgg
     5161 ttgcggacga ttaggcacga ttaatgaatt tacgattgcc tttgaagata aaaaaccaat
     5221 cggcatttta gagggagatt gggagactga tgaggttgtt agaaatatta ttgataaatc
     5281 tcacagggca gacgaagtaa gggataggat tgtttttgat tccgatccga aatctttgat
     5341 atcaaagatt attgaggtta taaaacttga taaagccagc aataagatgg ttttataaaa
     5401 tttattccgc catttgaatt tagtattcca taaattaaaa tagcagaaaa agtggataaa
     5461 atattatctt ggtatagaga atttctttta ccggcggttc ttttgtcagc tcttataata
     5521 ggagctggaa tgtttgctct tccttatctt tttgatcaag ccggatttat atcgggatta
     5581 ttctggctag ctcttttaac tgttgtggtc tcaacagtgc atctgatgta tctagatgtc
     5641 accaattata ctgaagggcg acatagattc gtcggttacg caagaattta tctcggtaat
     5701 tggggttttt tcatttcttt tattacaacg gctttgggga atatcctcgg actcctaata
     5761 tatattattc tcgcagtcag ccttattaaa gtaatcctgc ctaatctaaa cgattttatc
     5821 gctcttatta tattctggct tttttcaacg gctgcgtttg tggcgggcat taggagaatg
     5881 gctaatatag aattttgggg aacagttgga acggttatcg tggtgttcgc aatattcttg
     5941 ggaggagtcg gaggttttga tgcgggcgcg gtgaaactta ttgattttaa taattttctt
     6001 attccttacg gagcgatact tttttctctt ggtggcagag cggctatttc ttcaattaaa
     6061 acttatatta gcgaaaataa tttaagcagc aagcatctta ataaggctgt gattgttggc
     6121 actgtgatac cagctattct ttacctcctt tttattatag gtattattgg tctttctggc
     6181 ggatcggttt cagagaacgc ggttgcagga ctttatacaa tatcccctct ccttatttat
     6241 accatagcgc tcttgggcgt ttttgagtta tggacatctt atattttttt aggattagag
     6301 gccaaagaga tattaagtta cgatttaagc gtttctagat tttttgcatc agccatagtt
     6361 atgtttttac ccattgtttt atatattatt ggatttaata attttattgt cgcggtagga
     6421 atagctggcg gaatatttat cgctattgag gggatctttg tgatctctat gtggttgcgt
     6481 ttaagatcca gatctggttt agctctgaat atggcaagaa ttatggtagt tatgtttata
     6541 cttggagggc ttcaagagct tctatattga tttatcaacg ataatgccta tactacgggt
     6601 agttgaatgg ctgttgctat aaaaggattc ggcagctatt cattaaaatt tgaataatgg
     6661 aggtgctttg atgaaacgca atcagatcaa aacattcgac tacaggcgag gattcggttt
     6721 cacgatgaat ggagttttcg tccatatgaa tgatggacgg gttccggaac tggttggtga
     6781 taaggtggtt ttagggtcaa gcgtcatgcc tcctgatcgt tatccgaagc agggtgaccg
     6841 gatcgtattc gtcatcaaga ggattgacgg taaaagaaac gcggctcctt ggtgttttga
     6901 ggatgattat gaaagaataa aaaacaaact tagagagtca attgtttctg gcctcatttc
     6961 taaacttggc gatcaaggtt tccctccagg agagatgtct tggggagatg gatctatcaa
     7021 cgtcaggata tctatcagga ggagtcctaa ggggccaagt aatagaagaa aggttctttc
     7081 ttttgctgtg ggttaacaca aattaaggtt cgcatttttg cggccttttt tatttatgcg
     7141 ttttggccga tatagtagag aaaagataat tagcttacaa tatctataat gactttgttc
     7201 ggagagataa cgatagcttt aattctagcg tctgcgctgg gcatattttt ttatcttttg
     7261 cgtcaaccaa cgatcatcgg ttttatctta gcaggtattg ctataagtta ttttggctat
     7321 ttatccaccg agggaagatt tgagataata aatgttttat cctcaattgg cgttgctctg
     7381 cttctgttta tagtcgggtt agagatgagt tttaaagaat taaaagatgt tggcaagcag
     7441 tcaatcataa tagcttttgc tcaggctatc attactttta gcgtaggtta ttttatagcg
     7501 actcttcttg gcttttcggt aattgtttct atctatatcg gtatagcttt gactttttct
     7561 tcaacaatta ttttagtaaa gcttatttct gaaaaaaacg aattaaaaag tttatatgga
     7621 aggatcgctt taggttcctc tttgattcaa gatctaatgg ctgtaattat tctaattcta
     7681 ttggccggtt tgagcgatgg agtaagcgct ttgacatcgg catcggctgt tataaaaggg
     7741 ttattttttg tatttttagc aattttatct tccaaaatat tgccacgttt tttggatttt
     7801 ataggtacat caccggagct tctatatctt ttcagccttt cttgggcaat tggttgggca
     7861 gcttttttat cgtctccaat tataggactt tcgattgaaa tagggggatt tctagcaggc
     7921 ctttctcttg ctaattcatc tgaacatttt cagataggag caagattaaa gcctctcaga
     7981 gattttttct tgataatctt ttttatagga ttgggagcga aaatgct
//
LOCUS       DATDNZ010000066         7472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_993261, whole genome shotgun sequence.
ACCESSION   DATDNZ010000066 DATDNZ010000000
VERSION     DATDNZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993261"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VI432_02540"
     CDS             <1..594
                     /locus_tag="VI432_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY4497002.1"
                     /translation="VSALRSFLRQDPNIIMVGEIRDIETADISIQAALTGHMVLSSLH
                     TNDAPTAIPRLFDMNIPPFLVSAVLNTVVAQRLVRRICKDCIYSYKPPSHLVDAIRLE
                     ASELGIVKINIPKLIYAGKGCNICGDTGYKGRIGIFEILKISEGVRGLINSSNFSLDN
                     LRKLVRQDGMVSMFEDGMAKAEVGETTIEELLRVVRE"
     gene            718..1929
                     /locus_tag="VI432_02545"
     CDS             718..1929
                     /locus_tag="VI432_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4497003.1"
                     /translation="MKFRYIASEPSGKVVEGNIEATGPADVLEWMASQGLRPVSIKAI
                     GGVEIKGIKGVFSQDINIADKVFLTKYLALMLRVGTDLFRAIDILISDFDKPAVKALL
                     IEIKDSLAKGKPMYTTFTKYPKHFSSVFVNMIKAGEASGNLDSIFEDLSKSLEKEQAL
                     RSKLRAAFTYPIILVSLSFMILFLLASFALPRISEVFTSGGLEPPLFSKIVFAIGNFL
                     GDNFLLISIVLVGGVFGLWFFLKKSFIGQRVAGRILNKAPIINQVLYRISIQRFASTF
                     ASLLRSGMPIIEALETTADAVGSAELKSSLLRISREGIAKGLTIGESFQRETFFPRTV
                     VNLIAISEKAGHMEQILETLSDFYESEIDASVKTMLSVLEPALLLVIGLIVGTIALSI
                     IIPVYQLVGQI"
     gene            1948..2505
                     /locus_tag="VI432_02550"
     CDS             1948..2505
                     /locus_tag="VI432_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4497004.1"
                     /translation="MNREQLTINKEDKNYIKDINFSCKASNVSGQMFLRGLSLVELLV
                     TIGILAIIGSIAYSGFFVSKDTKTLESFTKRIVADLRSASSRAVAQEESDAWGIHFEN
                     PTGEDNDFYWLWHGNDYATGTSISRTSLPQSIRFTDPASGTSKDIVFAKLTGLPDSTA
                     DISIESISMGDSRIITINANGRIDF"
     gene            2565..3257
                     /locus_tag="VI432_02555"
     CDS             2565..3257
                     /locus_tag="VI432_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497005.1"
                     /translation="MLRKKGKLSHKQLRIRGQSLIEIIIGSVIGALVIGSAVSAVLLT
                     IRSNHDNRVVGTASSLMQDTVDSVRAVAGGGWHDLYNQSPKGSGEQYSISVAVGNFVI
                     QSGSENVEINGVVYTRYFTIENVCRDNSGRGSITGVTDSDGSSNGCASSGGLEDPSTQ
                     RITVKVDWPQGGELVNAGYLTRSDNKVTGFSDWSGEATDPPEVVTSPNNKYNTKDSNV
                     DISNTGEIKLSD"
     gene            3263..4225
                     /locus_tag="VI432_02560"
     CDS             3263..4225
                     /locus_tag="VI432_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497006.1"
                     /translation="MINFKKTILLFSVIASLSLAAVFAATNIDLTDKWAWNDARGWIN
                     FHSTNVVNILSNKIEGYAVFCGNDEVCTTPSADYILLDCGTSPGGDICEASNFKIFRD
                     GNGDLSGWAWNDGVGWISFCGGGDADVNCPGTISYGVNIDSVSGDFTGWAWNDVIGWI
                     DFNCDNDHDPAPGVQGICLDDGGHDFKVKTLWAPGAAAVGALLSNIFDTSVVGGVSLN
                     SIIWRGDINGAGNAVKFQIASSNCDGGSNPSACDAGDIWSFIGPGGTSGDTDVYEPLG
                     TGQSMPIVVSHHNNKQYYRYKVILDKAEGNSSPVVRDVIINWSP"
     gene            4229..4720
                     /locus_tag="VI432_02565"
     CDS             4229..4720
                     /locus_tag="VI432_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497007.1"
                     /translation="MKKESRSMNQEEVVDNSLFTIQDSKRGVVTIPLILVISGVVMEV
                     AIVGVIVATILGNTAFNERLHIESLSASRAGAEDAIVKIIRHGCSPRVTGCPSDDSLN
                     PDVIAVGDREADVWMVDHGSGAITVYSVGKALTRRAKTEVELVIDGISGRVDTQSFKE
                     VPL"
     gene            4727..6250
                     /gene="pilM"
                     /locus_tag="VI432_02570"
     CDS             4727..6250
                     /gene="pilM"
                     /locus_tag="VI432_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY4497008.1"
                     /translation="MKFKDYIRNFIEALMSSPSVGGLQISSSAIRFLELKKKTKTGVN
                     NSSVVRDVSIRLPHGIIDAGKIRDKEAFLDALKSVYSQTAKKKENINIVLTISAGEVY
                     VEAFNMPNLGGKNLHEAIDLNMQMISPSPIDEAYYSWQIVGPSDGPVEVLGAFAPKSV
                     IDEIVSILGEAGFRVVAIEFSTLSLVRNLQHLNILKIDEKLSYLALKITSDGIIFLVL
                     RNNNLYFNYFRGWENLADENGDISLGTITDIIKTEVDKILNFHNSRWGVQMKEIILIT
                     PALQNEIASFIRKQYSTFNVQIADSSNINLHGVMGAALRGLKSRIEDDDINLGSLTTI
                     QSFFRDQVLSFLSLWRNVFIAIGIFMVIVFVTVNILIGRYADEERSRNITFLQEREVA
                     ELEDLQLRAERFNSLVSLVFDARSKKQNIYPLLRRLDDSAGDRIEVEKLSIQSIGSVS
                     TITGLAPDHNAVTAFKNRLLEIEQLDEVDAPLSSIIPQISGKTAFIINFKITSLNFE"
     gene            complement(6256..6792)
                     /gene="pth"
                     /locus_tag="VI432_02575"
     CDS             complement(6256..6792)
                     /gene="pth"
                     /locus_tag="VI432_02575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY4497009.1"
                     /translation="MKIKIIAGLGNPGPEYEKTYHNLGLLFIQQFIAKYEGSIRQERL
                     YGYSKITKNEKEIIAIKPLTFMNDSGRPIKEAMKKFNCHPEELIVVHDDADLKIGNYK
                     LSFGSGSAGHNGINSIIKSIGTKDFYRLRIGIRDESPADREKAGEIVLKQIPKNHQVI
                     LDKTINKILEDGKIINAF"
     gene            6871..7416
                     /gene="lepB"
                     /locus_tag="VI432_02580"
     CDS             6871..7416
                     /gene="lepB"
                     /locus_tag="VI432_02580"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY4497010.1"
                     /translation="MKRFFVALWEVSEILIVALASIFIIYNFIAQPFLVQGASMEPNF
                     QSGNYLLVDELSYRFRDPIRGEVIVFINPNNESEFYIKRIIGLPTEKIDIINSQVFVD
                     GDKIDEYYLPLGADFKGDYSFILDSDEYFVMGDNRSQSFDSRNWGPLAKDKIVGAVRL
                     RFWPINKFQIFIDDYATQENN"
BASE COUNT         2180 a         1196 c         1725 g         2371 t
ORIGIN      
        1 gtttcagctc tcagatcttt tttgaggcag gacccgaata tcataatggt cggtgagata
       61 cgagatattg aaactgcgga tatttctatc caagccgctt tgacgggcca catggttttg
      121 tcgtcgcttc atacgaacga tgctccgact gcgatcccga gactttttga tatgaatatt
      181 ccgccgtttt tagtctccgc cgttttgaac actgtggttg ctcagcgatt agtgagaaga
      241 atatgcaaag attgcattta ttcatataag ccgccctcgc acctagttga cgctattcgt
      301 ttggaagctt cagaattggg tattgtaaag attaatattc caaagcttat ttatgctggt
      361 aagggatgca atatttgtgg tgacacgggt tataaaggaa gaataggaat ttttgagata
      421 ttgaagatta gcgaaggagt tagggggctt atcaacagct cgaatttttc tctagataat
      481 ttaagaaagc ttgtgagaca ggatggcatg gtgtctatgt ttgaagacgg aatggccaaa
      541 gcagaagtcg gagaaacgac catcgaggaa cttttgaggg tggtgaggga gtagtcccgt
      601 tagaagccgc gatcgcaata taataaatgt ataaatctat ggaggataag ataagtcgag
      661 acactatata taataattca attcaggtcg ccttgatcgc gatctgcttc taacgggatg
      721 aaatttcgtt atattgcatc tgaaccaagc ggaaaagttg tagagggaaa cattgaggct
      781 actgggccgg ctgatgtttt agagtggatg gcctcacagg gtttgaggcc ggtttccatt
      841 aaggctatcg gcggagtaga gattaaagga attaaaggcg tcttctctca agatataaat
      901 attgccgata aagttttctt aaccaaatat cttgctttaa tgttaagagt gggaacggat
      961 cttttcaggg cgatcgatat tcttatttct gattttgaca agcctgcagt caaagccctt
     1021 ttaatcgaaa taaaagactc tctggccaag ggaaagccta tgtatacgac ttttactaaa
     1081 tatccaaaac atttttcttc agtctttgta aacatgatta aggccggcga ggcttcagga
     1141 aatttagatt ctatttttga agatttaagc aagtcgcttg agaaagagca ggcgcttaga
     1201 tctaagttaa gagctgcttt tacatacccg ataatattag tctctctctc ttttatgatt
     1261 ctttttcttc tggcctcctt cgcccttcct agaatttctg aagtttttac atctggagga
     1321 ttggaacctc ctttattttc aaagatagtt tttgccatag ggaatttttt gggtgacaac
     1381 ttccttttaa tatccattgt tcttgtcggc ggagtgttcg gtctttggtt tttcttgaaa
     1441 aaaagtttta ttggtcagag agtggccgga agaattttaa acaaagctcc gattataaac
     1501 caggttttat atcgtatttc tatacagcgt tttgctagca cttttgcttc tctccttaga
     1561 tccggcatgc cgattataga ggcgcttgag accacggccg atgcggttgg atcggcggaa
     1621 ttaaaatctt ctctccttag aatttcgcgc gaggggatag ctaaaggatt aacaatcgga
     1681 gagtctttcc agcgcgaaac tttttttcca agaacggttg taaaccttat tgctatttcc
     1741 gagaaggctg gacacatgga gcagatttta gagactcttt cagattttta tgagtctgaa
     1801 atagacgcat ctgtcaaaac aatgctgtct gttttagagc ccgcattgct tttggttata
     1861 ggtttgatag tgggaactat tgctctttca atcataattc cggtctatca gctggtcggg
     1921 cagatatagg ttgcctgata caacacaatg aaccgtgaac aattaacaat taacaaggaa
     1981 gataaaaatt atataaaaga tattaatttt tcttgtaagg cgtcaaatgt gagtggtcaa
     2041 atgttcttgc gcggcttatc tcttgtagag cttcttgtca caattgggat tctagcgatc
     2101 attggctcta tagcttattc tggttttttt gttagcaaag atacgaaaac gcttgaaagc
     2161 ttcacgaaga gaatcgtggc tgatttgagg tccgcctctt caagagctgt tgcgcaagag
     2221 gaatcggatg cgtggggaat acattttgaa aatccaaccg gagaggataa cgatttttat
     2281 tggctgtggc atggaaatga ttatgcgacg ggaacaagca tctctagaac atctcttcct
     2341 caaagcatca gatttaccga tccagcaagc gggacaagta aggatatagt ttttgcaaaa
     2401 cttaccggtt tgcccgattc tactgcggac atctcgattg agagcatttc tatgggcgat
     2461 tcaagaatta ttacaataaa tgctaacggc cgaatagatt tttaataaat cttttataaa
     2521 agagccgctt agtaagatgc taggtttata gtagaattaa tataatgctc agaaaaaagg
     2581 gtaaattatc tcataagcag ttaagaatca gaggtcagtc tttaatagaa attattatcg
     2641 ggtctgtaat aggagccctt gtaatcggat ctgccgtaag cgcggttctt ttgactatac
     2701 gttctaatca tgataatcgc gttgtcggaa ccgcatcatc tttgatgcag gatacagttg
     2761 atagcgttag ggctgttgcc ggaggcggat ggcatgatct gtataatcaa tctcccaaag
     2821 gaagcggaga gcaatacagt atttctgtcg cagttggaaa ctttgttatt cagagtggat
     2881 cagaaaatgt agagattaat ggagttgttt atacaagata ttttacgata gaaaatgtat
     2941 gcagggataa tagcggtaga ggatcaatta cgggcgttac ggattccgat ggctcgtcaa
     3001 acggttgcgc ttctagcgga ggactggaag atccgtctac gcagagaata acagttaaag
     3061 ttgattggcc ccagggaggg gaactcgtta atgcgggata tcttaccagg tctgataata
     3121 aggttactgg ttttagcgat tggagcgggg aagctacgga tcctcccgag gtagttacga
     3181 gtccgaataa taaatataat acaaaagaca gcaacgttga tatttctaac actggagaaa
     3241 taaaattatc agactaaaca aaatgattaa ttttaaaaaa acaattttat tattttcagt
     3301 tatcgccagt ctttctctag ctgcggtttt tgccgcgaca aacatagatt tgaccgataa
     3361 gtgggcttgg aatgatgctc gtggttggat aaattttcat tcaacaaatg tggttaacat
     3421 cttgtctaat aagatagaag gttatgcagt cttttgtggc aacgatgaag tttgcacaac
     3481 gccatccgcc gattatatat tattggattg cgggacttct ccgggcggag acatttgcga
     3541 agcatccaac tttaagatat ttagggatgg aaacggagat ttatctggtt gggcgtggaa
     3601 tgatggagtc ggctggataa gtttctgcgg tggaggtgat gcggatgtta attgtccagg
     3661 aacgatatcg tacggagtaa atattgatag cgtgagcgga gattttaccg gttgggcatg
     3721 gaatgacgtt attggttgga tagattttaa ttgcgacaac gatcatgatc ctgctcccgg
     3781 agtgcagggt atttgtcttg atgacggagg gcacgatttt aaggtaaaaa ctctatgggc
     3841 tcccggcgca gccgcagttg gcgccttact atcaaatatt tttgatacaa gtgtcgttgg
     3901 tggagtttct ttgaattcta ttatctggcg cggtgatata aacggagctg gtaatgctgt
     3961 aaaatttcag atagcctcaa gtaattgtga tggcggatca aaccctagcg catgtgatgc
     4021 gggcgatata tggagcttca taggaccagg agggacgtcc ggtgatacag atgtttatga
     4081 gccgttggga acgggacaat cgatgccgat tgtggtttca catcataata ataagcaata
     4141 ttacagatat aaagttattt tagataaggc tgagggaaac tcatctccgg ttgtaagaga
     4201 tgtaataata aattggagtc cttaatatat gaaaaaagaa tcaagaagca tgaatcagga
     4261 agaggttgtt gataattcat tattcacaat tcaagattct aagcgcgggg ttgtgacgat
     4321 tcctcttatt cttgttattt caggagtcgt aatggaggtg gctatagttg gagttattgt
     4381 cgcgacaatt cttggcaaca ccgcttttaa tgaaaggttg catatagagt ctctttccgc
     4441 ctcaagagca ggagccgaag atgctatagt taaaataata agacatggat gctcgcctcg
     4501 tgtaactgga tgtccgtctg atgattcttt gaatccggat gtgatagctg ttggcgatag
     4561 agaggcggat gtttggatgg ttgaccatgg atcaggcgcg attacggttt attccgtggg
     4621 caaagcatta acgcgccgcg ccaaaacaga ggtagaatta gttatagacg gcattagcgg
     4681 ccgggttgat actcagtctt ttaaagaggt gccattataa tggaatatga aatttaagga
     4741 ttatattaga aattttattg aagccctaat gtcatcgcct tctgtgggtg gattgcagat
     4801 atccagttct gctatcagat ttttagaatt aaagaaaaaa acaaaaacag gtgttaataa
     4861 ttcgtctgtt gtcagagatg tttctataag gcttccccat ggaattattg acgccgggaa
     4921 gataagggat aaggaggcat ttttagatgc attaaaatca gtttattcgc agactgcaaa
     4981 gaaaaaagaa aatatcaata tagttttaac aatatcagcc ggtgaagtct atgtggaggc
     5041 ttttaatatg ccgaatctag gtggtaaaaa tttgcatgag gctattgatt taaatatgca
     5101 gatgatttct ccaagtccga tagatgaggc ttattacagc tggcagatag tagggccaag
     5161 tgatggtcca gttgaagttt taggagcttt cgctccgaag agtgtgattg atgagattgt
     5221 gtctatttta ggagaggctg gttttagagt ggttgcgata gagttttcta cgcttagcct
     5281 ggtcagaaac cttcagcatt taaatatttt gaaaatagat gaaaaactat cctatcttgc
     5341 tcttaaaatt acttcagatg gaattatctt tttagttttg agaaacaata acttatattt
     5401 taattatttc agagggtggg aaaatttggc ggatgagaat ggagatattt ctcttggcac
     5461 gataacagat attataaaga ctgaagtaga taaaatctta aattttcata acagcagatg
     5521 gggagtgcag atgaaagaaa ttattttaat aaccccagct ctacagaacg agatagcctc
     5581 ttttatcaga aagcaatatt ccacttttaa cgttcagata gctgactctt caaatattaa
     5641 tttgcatgga gtcatgggag ccgcactgag aggcttaaaa tccaggatag aggacgatga
     5701 tattaatttg gggagcctta ctacaatcca gtcgtttttt agagaccagg ttttatcttt
     5761 tttatcgtta tggagaaacg tttttattgc tataggcata tttatggtta ttgtttttgt
     5821 aacagttaat atattaattg ggcgatatgc agacgaggaa agaagtcgca atataacatt
     5881 tttgcaggag agggaggttg cggagctgga agatctacaa cttagggctg aaagatttaa
     5941 ttcattggtt tctcttgttt ttgatgcgcg gagcaaaaag caaaacattt atccgcttct
     6001 caggcgattg gatgattctg cgggagatag aattgaggtg gagaaattaa gcatacaatc
     6061 aattggttct gtaagtacga taaccggtct tgctccagat cataatgcgg ttaccgcttt
     6121 taagaataga ttgctggaga tagagcaatt agatgaagtg gatgccccgc tttctagtat
     6181 aattccgcaa attagcggta aaacggcttt tatcataaat tttaaaataa ccagtcttaa
     6241 ttttgaataa gtgatttaaa aagcgttgat tattttaccg tcttctaata ttttatttat
     6301 agttttgtct agaatgactt gatgattttt aggaatttgt ttcaaaacta tttctccggc
     6361 tttttcgcga tctgcaggac tctcatctct gatgcctatg cggagcctat aaaaatcttt
     6421 tgttccgata gattttatga tcgaatttat cccattgtgt ccggcagacc cggagccgaa
     6481 agataattta tagtttccta ttttaagatc ggcatcatcg tggacgacaa ttaattcttc
     6541 aggatggcag ttgaatttct tcatagcctc tttaattggt ctgccggaat cattcataaa
     6601 tgttaatggt tttatagcta taatttcttt ttcatttttt gttattttgg aatatccata
     6661 gagcctttct tgccgtatgg atccctcgta tttagcaata aactgctgta taaaaagcag
     6721 tcctaagttg tggtatgttt tttcgtattc ggggcccgga ttgcctagcc ccgcgataat
     6781 cttgattttc atgttttgat cgttataatg attatacttt ctggcttgat taatgtgaat
     6841 caatgatatt aagccggttt ttaaatatta atgaaaaggt tttttgtagc tttatgggag
     6901 gtatcggaaa tcttaattgt tgctttagcg tcaattttta taatatacaa ttttatagct
     6961 caaccgtttc tggtccaggg agcaagtatg gagcctaatt tccaaagcgg aaactatcta
     7021 ttagttgatg aattaagtta tagatttaga gatcctatta ggggcgaggt tatagttttt
     7081 atcaatccga ataatgaatc agagttttat attaaaagaa tcattggttt gccaaccgag
     7141 aaaattgata ttataaactc tcaggttttt gttgatgggg ataaaataga tgaatattat
     7201 ttgcctcttg gggctgattt caagggtgat tattctttta tccttgactc ggatgaatat
     7261 ttcgtgatgg gagataatag atctcagagc tttgattcaa gaaattgggg acctcttgct
     7321 aaggataaaa tagtcggagc agtgagactt agattctggc cgataaataa attccaaatt
     7381 tttatagatg actatgccac ccaagaaaac aattaagaaa aagacaggtt taaaagctga
     7441 gaagagcaag ccttctttta atttgccatc cg
//
LOCUS       DATDNZ010000067         5639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_721867, whole genome shotgun sequence.
ACCESSION   DATDNZ010000067 DATDNZ010000000
VERSION     DATDNZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721867"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VI432_02585"
     CDS             complement(<1..389)
                     /locus_tag="VI432_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4497011.1"
                     /translation="MKDINEILKISLDNIIEGCQIISPDFRYLYVNKSGARHGKSSVE
                     SLVGKRMVDVYPGIENTELFVQMKHSMDEKTPYRMENLFKFPDGSDGWFELRLDPVPE
                     GLMILSVDITEEKGARNKLEELNKKLE"
     gene            complement(818..1213)
                     /locus_tag="VI432_02590"
     CDS             complement(818..1213)
                     /locus_tag="VI432_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497012.1"
                     /translation="MALIQDLWGEEGLFGWSGNELQIVAERAFRNFVIDRPTTNDGYA
                     WFWGIQILNRSGAIAILFPWCQDWKKNDRTQSDRHIAVYTRGSIGEKKVRRLLYRLYE
                     AMEHRLEERAREQEEAEERRGEEAEQYSL"
     gene            1372..1764
                     /locus_tag="VI432_02595"
     CDS             1372..1764
                     /locus_tag="VI432_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY4497013.1"
                     /translation="MAKNNKNFTVKESMAGLGLFSNKSLKRGDFIIEYTGEKISSEEA
                     DRRGGKYLFTLNKKTVINAKARKNTARYINHSCRPNCYAEIDEDKNRINIYCKRKINK
                     GEEITYNYGKEYWEDFIGKNCRCEKCNA"
     gene            1805..2503
                     /locus_tag="VI432_02600"
     CDS             1805..2503
                     /locus_tag="VI432_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY4497014.1"
                     /translation="MKSHIEKHIKIGNYLKDIIFAANDGIVTTFAVVAGVVGASLSHE
                     IILIIGFASLFADAFSMATGNYLGTKTEQDFYNKESAEEAKEIEETPENELNEVREIL
                     RNKGYVGADVERMTELISSNKTYWRDFMLHEEIGIISPEKGSAFKNGLVTFISFLMAG
                     SIILIPYIFLNGDYAFQISILVGIITLFSVGALRKFFSDRSWFVSGLEMLIVGGSAAI
                     LAYLIGSFLGGIIG"
     gene            complement(2525..4630)
                     /locus_tag="VI432_02605"
     CDS             complement(2525..4630)
                     /locus_tag="VI432_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY4497015.1"
                     /translation="MEKEFEGFYTCPMHPEVKRDGPGICPDCGMNLVPLKKSPEHPPD
                     NREYDKHAGHKTNVFKIKFLVSLALTIPVLFYAEVLQEIFNYKAPDFYGSDYVPLIFA
                     SIIFFYGGWIFIVSAYRELKAKLPGMMTLISIAISTAYIYSVYITFIGGDETLFWELS
                     TLITVMLLGHWMEMRAVSAASGALKELSKLLPDKAELIQGGSTIMIFLRELKEGDLVL
                     VKPGGRIPADGIVFEGSSDVNESIITGESKPVAKKEGDEVIAGSTNGDGSLKVRVNKI
                     GEKTFLSGVMRLVVEAEASKSKLQILSDRAAYYLTIIAVITGVATFAFWILLSDFSFA
                     IERMVAVLVIACPHALGLAIPLVASISTTKAARSGFLVKRRLALESARNINTILFDKT
                     GTLTKGEFGVSDILFTEGYSDKTVLKYSASINNNSEHPIAVAVVKSAKEKGLEFFNVS
                     KFKRIPGKGVEAEIDGLAILVGSDRLLEERGLYLDESLKIRIDELTKQGKTINFVILG
                     GKVIGAIALTDVIRDESREAIKTLKDMGIKPVMITGDADDVAKWVAGELGIDEYFSRV
                     LPEQKSEKVKLLQSRGLRVAMVGDGVNDAPALTQADLGIAVGAGTNVAIESAGIILIK
                     NDPRDIVKIIKLSRLTYIKMIQNLLWATGYNVVAIPLAAGILISWGILLQPALAAFFM
                     SASTVIVAVNATMLRNKSL"
     gene            4673..5329
                     /locus_tag="VI432_02610"
     CDS             4673..5329
                     /locus_tag="VI432_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497016.1"
                     /translation="MIFGYAHVYILNRRIEIIRTALLSVYKKEGIVEFARELQKLGWS
                     ILASSGTHKILAANGIFATDVASIVGEPILGHQVVTLSREIHAGLLAKDTPEDKKELE
                     RLGIKKIDLVCVDLYPLEDAIANGEPYEKVIELTDIGGPTLLRSAAKGGRIVIADPSR
                     RKVVLDWLRAGEPNGDVFRMSLAALAERIVGDYCLTSGEYLNGRIRAETIKRTQIFLV
                     "
     gene            5458..>5639
                     /locus_tag="VI432_02615"
     CDS             5458..>5639
                     /locus_tag="VI432_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497017.1"
                     /translation="MDHQFKIGYKLIIGGLLTLSLILGGLLWLTLQELQTIETQDEQP
                     LYLRAEEGVAVTEYII"
BASE COUNT         1755 a         1307 c         1051 g         1526 t
ORIGIN      
        1 aattctaatt ttttatttaa ctcttctaac ttattgcgcg cgcccttctc ttctgttata
       61 tccactgata aaatcatcaa tccttctggg actggatcta aacgcaactc aaaccatcca
      121 tcgcttccat caggaaattt aaataaattt tccattctat aaggcgtttt ctcatccata
      181 gaatgcttca tctgcacaaa aagctcggta ttctctatac caggataaac atctaccatc
      241 cttttgccga ccaatgactc aacactgctt ttgccgtgac gggcaccaga tttattaaca
      301 tatagatatc gaaaatccgg acttataatt tggcacccct ctattatatt gtctaacgag
      361 atcttaagaa tctcgtttat atctttcata ggcctagctt gaatttaaaa aataatattt
      421 atagtagtga tgatctaata gaaataatta ggttgtcggg caatttttaa taataaaaat
      481 cattcggatt taatcccaaa aatattataa caaattaatc atataaaatc gagttaaaaa
      541 taaatgcccg ccacaatctg cttctagcaa aattatgacg ggcgggattt ctggacagag
      601 aatggaaaaa atcagtcccg gcgaagatcc cgaacacaac agcaacagac atgacccttc
      661 atcgcaaaag agggcgcatc accgatcatg ccacggacgg caagatcgcg cagatagacc
      721 ctccaagcgc ggtgagagcg ctgtttgtca atcttccgag aacggcgctt cggtcggtaa
      781 acggtccgac tcatataaat gctttcatgg acacctctta gagagagtat tgctcagctt
      841 cctctccgcg gcgttcttcc gcctcttctt gctcgcgcgc ccgctcttca agccgatgtt
      901 ccatcgcctc atagaggcga tagagcagac gacgaacctt cttctctcct atgcttccac
      961 gggtgtaaac cgcgatatga cgatcggact gagtgcgatc attctttttc cagtcctgac
     1021 accaagggaa cagaatggca atagcaccac tccgattcag gatctggatc ccccagaacc
     1081 atgcgtaacc atcgtttgta gttggccgat caataacaaa gtttctaaaa gctctctcgg
     1141 caacaatttg gagttcatta ccactccagc caaagagacc ctcttctccc caaagatctt
     1201 ggataagagc caattcgtcg gggaaggtcg gaccattcca accatccaga cgcgcgttct
     1261 ctttttgttc tctggacatt tttcactctc ctatgaaaaa gaactttttc gcgcttgcgc
     1321 gaaaactaaa gtaagattga cataattatt atctcatgtc aaattttgac catggcaaaa
     1381 aacaacaaga atttcaccgt taaagaatct atggcgggct tgggactctt ctccaacaag
     1441 tcactcaaaa gaggcgattt tataatcgag tacaccggag aaaaaatctc aagcgaagaa
     1501 gcagacagac gcggaggaaa atatcttttt acactcaaca aaaaaacggt tattaacgcg
     1561 aaagcgcgaa aaaatacagc aagatatatc aaccactcct gccgaccaaa ttgctatgcc
     1621 gagatcgacg aagataaaaa tagaataaac atctattgca aacgtaaaat aaataaaggc
     1681 gaggaaataa catataatta cggcaaagaa tattgggagg atttcatcgg caaaaattgc
     1741 agatgcgaaa aatgcaacgc ttgaatcctt ctactttcat aacttttatt ataccatctc
     1801 atatatgaag tctcatatag aaaaacatat caagatagga aattatctaa aagatataat
     1861 ctttgctgcc aatgacggca tcgttaccac tttcgcggtt gtcgccggcg ttgtaggagc
     1921 gagtctttcg catgaaataa ttcttattat cggcttcgca agcctttttg cagacgcctt
     1981 ttcaatggca accggaaatt atttaggcac gaagacagaa caagatttct ataataaaga
     2041 gtcggctgaa gaagccaaag agatagaaga aacgccggag aatgaattaa atgaagtcag
     2101 ggaaatatta agaaataagg ggtatgtcgg agcggacgtt gaacgcatga ctgaacttat
     2161 atcttccaat aaaacatatt ggcgagattt catgttgcac gaagaaattg gaataatctc
     2221 tccagaaaag ggatcagctt tcaagaatgg attagtcaca tttatctctt ttctaatggc
     2281 cggaagtata attcttatcc cctacatatt cttaaacggc gattatgcgt ttcaaatatc
     2341 aattctggtt ggaataataa ccctattctc cgtcggcgcg cttagaaaat tcttttcaga
     2401 tcgatcttgg tttgtatcgg gactcgaaat gcttattgtt ggcggatctg cagcgatact
     2461 tgcctactta ataggatctt tccttggtgg aattatcggc taattaagca ttaacttata
     2521 aaaattatag agatttattt ctaagcattg tcgcgtttac tgcgacaata acagttgatg
     2581 cagacatgaa aaatgccgcg agtgccggct gtagcaatat accccaagaa atcaatattc
     2641 ctgcggcaag aggaatagca acaacattat atcccgtcgc ccataataaa ttctgaatca
     2701 tctttatata agtcaggcga gacagcttga ttatcttaac tatatctctc ggatcgtttt
     2761 ttataagtat tatgccagct gactcaatcg caacatttgt gcctgctcca accgcaatac
     2821 ccaaatctgc ttgagtcaga gccggagcgt cattaactcc gtcgccaacc atagcaactc
     2881 taagccccct agactgcaac aatttaactt tttctgattt ttgttccggc aaaaccctag
     2941 aaaaatattc atctattccc aattctccgg caacccactt tgccacatca tccgcatcac
     3001 cggttatcat aaccggctta atacccatat ctttaagcgt ttttatcgct tcgcgcgatt
     3061 catcgcgaat cacatcagtc aaggctattg cgccgattac tttaccacct aaaattacaa
     3121 agttaatagt cttaccctgc ttagttaatt catcaatcct gatctttaaa gactcatcta
     3181 aatacagccc tctttcctca agcaatctat cgctgccgac taaaatagcc aacccgtcaa
     3241 tctcagcctc gactcccttc ccaggaattc tcttaaattt tgaaacattg aaaaattcaa
     3301 gtcctttttc tttggctgat tttacaacag caacagcaat aggatgttct gagttgttat
     3361 taatagaagc tgaatattta aggactgttt tatcgctata accttcggta aataatatgt
     3421 ctgacacgcc gaattctccc ttagtcaggg tgccggtttt atcaaataaa atggtattaa
     3481 tatttctggc agactctaaa gctaatcttc tctttaccaa gaatccgctt ctggcggctt
     3541 tcgtcgtaga aattgatgcg acaagaggaa tggcaagccc aagcgcgtgc gggcaagcga
     3601 tcacaagaac cgcaaccatc cgctcaatcg cgaaagagaa atccgagaga agaatccaga
     3661 aagcaaaagt ggccacgcca gttataacgg cgataattgt aagataatat gcagccctgt
     3721 ctgacaagat ctgcaactta gacttagacg cttcagcctc taccaccaac cgcatcacgc
     3781 ccgacagaaa agtcttctcg ccgattttat taaccctaac cttcaatgaa ccgtctccat
     3841 ttgtcgaacc ggctataact tcatctcctt cttttttagc aaccggcttc gattctccgg
     3901 taatgattga ttcatttacg tcagacgaac cctcgaaaac aatcccgtct gcaggaattc
     3961 ttccgccggg ttttaccaaa accaaatcac cttcttttaa ttctctcaaa aatatcatta
     4021 tagttgatcc tccttgaatc aactccgcct tatcgggtaa aagcttggaa agctctttca
     4081 gcgctccgct cgcggcagaa actgcccgca tctccatcca atggcctaag agcatcaccg
     4141 ttatcaaagt tgaaagctcc caaaacaaag tttcatcccc gccgataaaa gttatataaa
     4201 cgctataaat ataagcggtc gaaatagcga tagagatcaa ggtcatcatg cccggcaatt
     4261 ttgctttcag ttctctataa gcagaaacga taaaaatcca accgccatag aaaaagatta
     4321 tcgaggcgaa tattaatggg acgtagtcag aaccgtaaaa atccggagcc ttataattaa
     4381 aaatttcctg cagaacttct gcgtaaaaca gtacaggaat agtcaaagcc aaactaacta
     4441 agaatttaat cttaaaaaca ttcgttttgt gtcctgcgtg tttatcgtat tcgcggttat
     4501 ccggcggatg ctccgggctt tttttaagag gcaccaaatt catcccgcaa tcggggcaaa
     4561 tcccaggccc atccctctta acctcggggt gcataggaca ggtataaaat ccctcaaatt
     4621 ccttttccat ctggatttat tatgtcataa atgaattcgc taataaaata atttgatttt
     4681 tggatatgcg catgtttaca tattgaacag gaggattgaa ataataagaa cagccctact
     4741 gtccgtttac aagaaagaag gaatcgtaga gttcgctcgc gagcttcaaa agctcggatg
     4801 gagcattctt gcatccagtg gaacgcataa aatattggcg gccaatggca ttttcgcgac
     4861 agatgtcgca agcatcgtcg gcgaaccgat cttgggacat caagttgtaa ctctttcgcg
     4921 cgaaatccac gcaggacttc tcgcaaaaga cactccggaa gacaagaagg aactcgagcg
     4981 tctcgggatc aaaaaaatcg atctcgtctg cgtagaccta tatccgttgg aagatgcgat
     5041 cgccaatgga gagccctatg aaaaagtcat agagctgacc gacatcggcg gaccgacgct
     5101 tctccgatct gccgcaaaag gcggacgcat cgtcatcgct gatccatcta gacgcaaagt
     5161 ggttctcgat tggttaaggg caggcgaacc aaatggagac gtcttccgca tgagcctcgc
     5221 agctcttgct gagagaatcg ttggagacta ctgcctaaca tcaggagagt atctaaacgg
     5281 gcgcatccgc gcagaaacga tcaagcgcac acaaatattc cttgtctgaa aatacaaaac
     5341 caccaaatta tcggtggtta ttttattcaa aaaaatacct ctataaaata atcgaaaacg
     5401 tcattatgaa ttcgcaaaat ttaacatttt ggtccaatcc atttattatt aaccccgatg
     5461 gaccatcaat ttaaaatcgg ctacaagcta atcattggcg gattattaac cttatctcta
     5521 atactcggcg gcttgctctg gttaacccta caagaattac agacaattga aactcaagat
     5581 gaacaacctc tttatttgcg ggctgaagag ggcgttgcag tgaccgagta tataataag
//
LOCUS       DATDNZ010000068         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2055195, whole genome shotgun sequence.
ACCESSION   DATDNZ010000068 DATDNZ010000000
VERSION     DATDNZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055195"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /gene="infA"
                     /locus_tag="VI432_02620"
     CDS             <1..175
                     /gene="infA"
                     /locus_tag="VI432_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY4497018.1"
                     /translation="EALPATLFKVRLEEGQEILAHLSGKMRVNFIRILPGDRVVVELP
                     DANSSRGRIVYRE"
     gene            201..314
                     /gene="rpmJ"
                     /locus_tag="VI432_02625"
     CDS             201..314
                     /gene="rpmJ"
                     /locus_tag="VI432_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY4497019.1"
                     /translation="MKVRASVKKMCRDCKIVRRRGRVSVICKNPKHKQRQG"
     gene            414..800
                     /gene="rpsM"
                     /locus_tag="VI432_02630"
     CDS             414..800
                     /gene="rpsM"
                     /locus_tag="VI432_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY4497020.1"
                     /translation="MPRIAGINIPENKNIEISLCSIYGIGRSRARGILKAAQVNAQRK
                     TSELSAVEVARIRDFIEKNYRVEGELRQEKLANIKLLKDIASYRGIRHAKRLPVRGQR
                     TKTNSRTIRGNVRRTVGSGRRKLEKT"
     gene            833..1291
                     /gene="rpsK"
                     /locus_tag="VI432_02635"
     CDS             833..1291
                     /gene="rpsK"
                     /locus_tag="VI432_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY4497021.1"
                     /translation="MGKKHIILKTDEELIREREAVEAGVQRAVTAIKPIVKKLANAQV
                     HILASYNNVIVSLTDEDGNVMAWSSSGTLGFRGSKKATPFAASRVAEAVAEKGKTLGV
                     VSVSVFVKGIGSGRESAIRSLASHGLDIRAIQDVTPIPHNGPRPRKPRRV"
     gene            1337..1966
                     /gene="rpsD"
                     /locus_tag="VI432_02640"
     CDS             1337..1966
                     /gene="rpsD"
                     /locus_tag="VI432_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4497022.1"
                     /translation="MIGAKCKICRRAGQKLFLKGDRCFSPKCAIVRRPYPPGVHGQKS
                     RPSRSEFGLQLAEKRKLEASYHIDERDLKRIVKEGSSTRPQNLDIADMIVNRLESRLD
                     NVIYRLGFVVSRSVARQLVSHGHFYINDRRMDIPSYLLRIGDKVSIRPASKDKSPFKN
                     MASHLEKYTPPSWIELDKQELNGKVIGVPNRSEIGLPVDLSMIVAYYSR"
     gene            2002..2757
                     /gene="rpoA"
                     /locus_tag="VI432_02645"
     CDS             2002..2757
                     /gene="rpoA"
                     /locus_tag="VI432_02645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY4497023.1"
                     /translation="MISLPTKPAIIKHEGNNAVFEITQLYPGYGVTLGNVLRRILLSS
                     LPGAAVTEVKIRNIDHEFTSIPGVKENVVDLLLNVKKLRFRVNGNEPQNVKLEVKGER
                     KVSGVDLEAPGQVEVVSTQEHIATLTDKKAELYMELTVESGVGYLPVEMRKKEKLSVG
                     NISVDAIFTPVRKVNFSVENMRVGDRTDYNRLILEIETDGTISPENALIGAATILVEQ
                     AAAIADWSLPPREGLELKTLSDGVAKKTKRSKK"
     gene            2778..3125
                     /gene="rplQ"
                     /locus_tag="VI432_02650"
     CDS             2778..3125
                     /gene="rplQ"
                     /locus_tag="VI432_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY4497024.1"
                     /translation="MRKRKRGRKFHRESSQRKAMMSHLTSALFINGKIKTTEAKAKEL
                     KPLAEQMITRAKKGTLANRRQLAQTLPNKVVKVLVDEIAPRLSARNGGYTRITKLAAR
                     KSDGASQAVIEIM"
     gene            3139..3504
                     /gene="rplM"
                     /locus_tag="VI432_02655"
     CDS             3139..3504
                     /gene="rplM"
                     /locus_tag="VI432_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4497025.1"
                     /translation="MQRKNITIDASGKKVGRLAVQIANILRGKDASNFAPNLDPGVSV
                     SVINVGRMEFSKRKQRSKTYWRHSGYLGGIKFTSAEYLWKKNPNEVLRRAIWGMLPSN
                     RLRARMMRHLEILSGEKNK"
     gene            3624..4046
                     /gene="rpsI"
                     /locus_tag="VI432_02660"
     CDS             3624..4046
                     /gene="rpsI"
                     /locus_tag="VI432_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4497026.1"
                     /translation="MKKTSEDKGKEKFFSAVGRRKTAIARVRLFTKGDKGAVVNGKSL
                     DDYFHNNLEFATLAASSLSRMKVTDKFRAIINVSGGGLHAQAEAIRHATARALVLFNP
                     DFRKRLKRAGFLTRDSRMVERKKYGLKKARRAPQWQKR"
BASE COUNT         1361 a          765 c          938 g         1108 t
ORIGIN      
        1 tgaagcattg cccgctacct tatttaaggt gcgccttgaa gaaggacaag aaattttggc
       61 ccatctatca ggcaaaatgc gagtaaattt catacggatt ttaccaggag atcgtgttgt
      121 agttgaactg ccagatgcta attcttctcg cggcaggatt gtttatcgcg agtaaattta
      181 gcgtcgcaag tctttatttt atgaaagtta gggcctctgt taaaaaaatg tgtcgggatt
      241 gtaagatagt ccgaagacgc ggacgggttt ccgttatctg caaaaatcct aagcacaaac
      301 aaagacaagg ttaagcggcc cccggtataa atgaaaaagt aaaataaaag atggtcgtct
      361 atggcaataa accaaaattt taaattttcc atttttaatt ttaaatttga aacatgccgc
      421 gtattgcagg aataaatata ccagaaaata aaaatataga aatttccctc tgttccattt
      481 atggtatcgg acgtagtcgg gcgcgtggga ttttgaaggc cgcgcaagtc aatgcgcagc
      541 gtaaaacttc ggaattatct gccgtagaag ttgcgcgcat ccgcgatttt atagagaaaa
      601 attatcgtgt ggagggagaa ttgcgtcagg aaaaattggc caatattaag cttctaaaag
      661 atattgctag ctaccgtggt atacgccatg ctaaacgcct tccagttcgc ggtcagcgca
      721 ccaagactaa ttcacgaact atacgtggta atgtaagacg caccgtcgga agtggtagaa
      781 ggaaactgga aaaaacatag tttatcataa tttcgaatgt agaattttta ttatgggtaa
      841 gaaacacata attctaaaaa ctgatgaaga gcttattcgc gaaagagagg cggtggaagc
      901 tggtgtacag cgtgctgtca cggcgattaa acctatagtt aaaaaactag ccaacgcaca
      961 ggtacacata cttgcttctt ataataatgt tatagtttca cttactgatg aagatgggaa
     1021 tgttatggcg tggtcatcct ccggaacatt gggttttaga ggatcaaaga aagctacccc
     1081 ctttgcggcc tcgcgagttg ccgaagcggt cgcagaaaaa ggtaaaactt taggcgtcgt
     1141 gtccgtttca gttttcgtaa agggcatagg tagcggcaga gaatcggcta tacgttctct
     1201 agcttctcat ggtcttgata tacgagccat tcaggacgtt acgcctattc cgcataatgg
     1261 gccacgacca cgaaagccgc gcagagtata aaatatcctt aaacaattat ttaattattt
     1321 ttaaatttaa ataaaaatga taggcgcaaa atgtaaaatt tgcagaaggg ctggtcaaaa
     1381 attgtttttg aaaggggaca ggtgttttag tcctaagtgc gctatagtca gaagaccata
     1441 tccaccgggg gttcacgggc aaaaatcacg gccttcccgt tccgaattcg gactacagct
     1501 tgcggaaaag cgcaagcttg aagcttctta tcatatcgat gaacgcgact taaaaaggat
     1561 agtcaaagaa ggcagtagca cgcggcctca aaatttggat atagccgata tgatagtaaa
     1621 ccgtctcgaa tctcgtttag ataacgtaat ttacagatta ggttttgtcg tatcaagatc
     1681 cgttgcgaga cagctggttt cacacggcca tttttatata aatgatcggc gcatggatat
     1741 tccttcgtat ttacttcgca ttggtgataa ggtttctata cgtccggcaa gtaaggataa
     1801 gagtccgttt aaaaatatgg cttcgcatct tgaaaaatac acccctcctt cgtggattga
     1861 attagacaaa caagaattaa acgggaaggt tattggcgtt cccaaccgta gtgaaattgg
     1921 cttgcccgtg gatttgagca tgattgtagc ttattattct cgttaatatt ttatctttta
     1981 ttttgttaaa tcatcaaaat tatgatttct ttaccaacca aaccagcaat tattaaacac
     2041 gaaggcaata atgccgtatt tgaaataaca caactttatc caggttacgg tgtaacatta
     2101 ggcaacgttt taagaagaat actgttatct tctttgcctg gagccgcggt gaccgaagtt
     2161 aaaataagga atattgacca cgagttcaca tctataccag gagtaaaaga aaatgttgtt
     2221 gatcttttac ttaacgtcaa gaagcttagg tttagagtaa acggtaatga accacagaat
     2281 gttaagcttg aagttaaagg agaaagaaaa gttagtggag tggatcttga agctcccggt
     2341 caagtcgaag tggtttcaac tcaagaacac attgccactc ttactgataa gaaggcagag
     2401 ttgtatatgg agctgactgt tgaaagcggc gtgggttatc tgccagttga aatgagaaaa
     2461 aaggaaaagc tttctgtcgg caatatttcc gtggacgcca tttttactcc agtcaggaag
     2521 gtcaattttt ctgtagaaaa tatgcgcgtt ggcgacagaa cagattacaa tcgtttgatt
     2581 ttagaaatag agaccgatgg aactatttct cctgaaaatg ctttgatcgg agcggcaaca
     2641 attttggttg aacaggctgc ggcaatagcc gattggtctc tgccaccaag ggaaggattg
     2701 gaattgaaaa cattgagtga cggcgtagca aaaaagacta aacgttctaa gaagtaattt
     2761 ataaggataa ccgcgtcatg cgcaagagga aaagagggag aaaatttcat cgcgagagtt
     2821 cacaaagaaa agctatgatg tctcatttga catcagcttt attcattaac ggaaaaataa
     2881 aaactaccga agccaaggca aaagaactga agcctctagc cgagcagatg ataacacgag
     2941 ctaaaaaagg aaccttggca aatcgcaggc aactagcaca aacacttccc aataaagtcg
     3001 ttaaggtttt ggttgatgaa atagcgccga gactatccgc aagaaacggc ggctatacgc
     3061 gtattactaa attggctgcc agaaaaagcg atggcgcgtc acaggccgtg atagaaatca
     3121 tgtaaacatt ttccaaatat gcagagaaaa aatatcacca ttgatgcttc cggcaaaaaa
     3181 gtaggccgtt tggctgttca gatagcaaat attttgcgcg gtaaagacgc gtctaatttt
     3241 gcgcccaatc ttgatcccgg cgtcagtgtc tcggttatca atgtgggcag aatggaattt
     3301 tccaaacgaa aacagcgcag caagacatat tggcgacatt ctggttatct tggaggtatt
     3361 aaatttacct ccgctgaata tctatggaag aaaaacccca atgaagtatt acgtcgagcg
     3421 atttggggca tgttgccgag taatcgttta cgggcaagaa tgatgagaca tttggaaatt
     3481 ttatctggag aaaagaataa ataaagttgt aaaatatgcc caaaaaggtt gctacaacaa
     3541 agaaaaagaa acctaaagct aaaattactc attccgtatc aaaaacggca gaacattctg
     3601 ttattgagga aattaaacca gatttgaaaa agacgagcga agataaaggg aaagaaaaat
     3661 tcttttcagc cgtgggaaga cgcaaaaccg ctatcgccag agtccgtctc tttactaaag
     3721 gagataaagg tgcagtagtc aacggtaaat ctttggatga ctatttccac aataatcttg
     3781 agttcgccac gctcgcggct tcatcattaa gtcgcatgaa ggttacagat aaatttcgcg
     3841 ctataatcaa tgtttctggt ggcggtcttc atgcgcaagc cgaagccata aggcatgcca
     3901 cagcgcgagc ccttgttttg tttaatcccg acttcagaaa gagattaaag agagccggat
     3961 tcttaactcg cgattcacgc atggttgaac gcaagaaata cggattaaaa aaggctcgcc
     4021 gagccccgca gtggcagaaa cgatagcatt gttgacagtg ttattaattt gttaggacta
     4081 acagaaaata aaagatttaa aaatcacttg atgataagtg gtttttatat atgtgtctaa
     4141 aatccaaata aaccttcaat gcgcggaact tc
//
LOCUS       DATDNZ010000069         8397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1537114, whole genome shotgun sequence.
ACCESSION   DATDNZ010000069 DATDNZ010000000
VERSION     DATDNZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537114"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VI432_02665"
     CDS             complement(<1..521)
                     /locus_tag="VI432_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497027.1"
                     /translation="MNKKRRPRKNVVIDIRPPRGSSRFSTASPYFSEGDIYSRPKLEF
                     GDRFKILKVLILSILIIGVLAVSSYAFYLWNYKDDAFSSGLKIYDSFKLAAESLLDLE
                     TDKTAGFIDLIKEEIDKIGYRIKPLEIVPFLNEAPGALGLIKDVAEVVASLNTEIAIL
                     KEDGADMLFSDDG"
     gene            complement(533..1138)
                     /locus_tag="VI432_02670"
     CDS             complement(533..1138)
                     /locus_tag="VI432_02670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY4497028.1"
                     /translation="MNYPLWKRIEDILGALAGMLLILPLAPFIILAIKLEHPEASFIV
                     RLPRISEGRIIYVYKFRNMVPEADKMKDGLRHLNERNDGPFFKIANDPRVTKVGRILR
                     KFRIDEFPQLINVLKGELSLVGPRPHEEEEIEKYPNEYKLIAKSRAGVTGLSQVNGAS
                     SLPFLKEVELDKFYIDHVSLWIDVKILFKTLSILFSDPNAV"
     gene            1172..2293
                     /locus_tag="VI432_02675"
     CDS             1172..2293
                     /locus_tag="VI432_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4497029.1"
                     /translation="MPTIFNKKPRVALLHDYLNQYGGAERVLETFIELFPDAPIYTLF
                     YDKELMGGRFNNKIIKTSFLDKKIIRRNHRFFIPLMPKAAESIDLGDNYDFIISDSAG
                     WSKGINYKNAVHINYLHCPLRYAWEPKRYLDTLIPEYLIKSAGPIIKYLRDWDKETGL
                     KPDILITPSKFTAEKVGRFYNRDTKILHPPIDTDIFFYEPSIEEKIEPYFLTFGRIVH
                     QKRFDLLVSAFNKLDVKLKIVGSGLDIKNIERIKSSPGIDLLNWVEDNELRKLISGAK
                     ATIFAQIEDFGMVAAESIACGTPVIAFNEGGFKEIIKEGENGIFFNEQKEESIIDALK
                     KFEKTKFDREAVASSSKQFHKKTFIKDFKKILSDAGLYI"
     gene            complement(2294..2560)
                     /locus_tag="VI432_02680"
     CDS             complement(2294..2560)
                     /locus_tag="VI432_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEY4497030.1"
                     /translation="MEKINSDKYKLVPISERARSFKLGKYEHFKGGQYEVICLARDSE
                     DKERELVVYQSLDQGHIWVGPLDMFMEDVVIGGKSAPRFRYLGI"
     gene            complement(2614..2883)
                     /locus_tag="VI432_02685"
     CDS             complement(2614..2883)
                     /locus_tag="VI432_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEY4497031.1"
                     /translation="MAIIKKKIWPEYFDSMASGKRKYDMRLQEFEIKEGDTLILEEWD
                     PKTKEYTGRSVEKKVTHVSKFRIDDSPWWPEDEIIEKGLQIISIE"
     gene            3092..3361
                     /locus_tag="VI432_02690"
     CDS             3092..3361
                     /locus_tag="VI432_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497032.1"
                     /translation="MMRETTSPEEAKEARRRRGRAIILIEKLRNQPGTYFTLGQITWF
                     TRLTDPSKPFNPIEEDLVKLQEMTDQASQDTEAFSKVASGRARCT"
     gene            complement(3482..3826)
                     /locus_tag="VI432_02695"
     CDS             complement(3482..3826)
                     /locus_tag="VI432_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEY4497033.1"
                     /translation="MENNEIQYSDFEKVDIRVGKIIKVEDFPEARKLMYKFTVDFGPE
                     VGIKISAGQFTKNYTKDELMGRLIIGCINLVPKKIGPFTSEFITLGLTDRDGNAVLLS
                     PDKDVSLGERMY"
     gene            complement(3816..4154)
                     /locus_tag="VI432_02700"
     CDS             complement(3816..4154)
                     /locus_tag="VI432_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY4497034.1"
                     /translation="MGNKDMQFEDLMRFIKKEDKRLRRYYGNYKDEEKRILARTVKLS
                     EELGELCDAVLRHNAMQRKGKMKKSGDNLSEEFADVLITTLLLAEAMDIDIDRALIKK
                     MKKIDKRYGK"
     gene            complement(4144..4416)
                     /locus_tag="VI432_02705"
     CDS             complement(4144..4416)
                     /locus_tag="VI432_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497035.1"
                     /translation="MESNMKDIERKIQVIENRNKRVEVDKAWETSWTRRFLLMAFTYL
                     AIGTYMWAISVPKPWLNAVIPAVAFMLSTLTMPFFKKWWIKNNNGK"
     gene            complement(4406..6937)
                     /gene="secA"
                     /locus_tag="VI432_02710"
     CDS             complement(4406..6937)
                     /gene="secA"
                     /locus_tag="VI432_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY4497036.1"
                     /translation="MASLIKKIFSSVPRPPKELELINNLEKEIEILDDAGLRAESLKI
                     KEAIFSGADLNEFLPRAFALVREAAKRALSQRHFDVQFWGGIVLHQGAIAEMSTGEGK
                     TLAATAPTYLNALSGKGVHLITVNDYLAKRDAVWMGQVYNLLGLKTGCIAHNSAYIYD
                     PEWRASSDEKKEIDKDRDEKGGFLVHEEYLRPVSRKEAYSADITYGTNHEFGFDYLRD
                     NLVQSKEMKVQRELNFAIIDEVDSILIDEARTPLIISVPDKESSEFYKVFARAVRNLK
                     QDEDYAVDEKLKTVEINNSGIDKIEGMLGIKDIYAPENLRLVHYLEASLKARALFDRD
                     KDYVVRSGEVILVDQFTGRLMLGRRYSSGLHQAIEAKEGVLVKEESKTYAQITIQNYF
                     RLYKKISGMTGTAQTSAEEFHKVYGLEVVSVPTNKPLVRADLPDVIFRTQEAKYKAIV
                     EEIRDRNKKGQPILIGTVSIEKNELLGNILKREGINCEVLNAKNHEREGSIIAQAGKS
                     GSVTVATNMAGRGVDIILGGNPPDSEEARKVRDLGGLHVIGTERHEARRIDNQLRGRA
                     GRQGDPGSSQFFVSLEDDIARIFGGDRVKGLMDKFNMPDALPASKMVSGSIQQAQSKV
                     EGFNFDMRKHLLEYDDILNKQRLKFYEIRAEVLDSLENEVEIKTIVRRYVDSQFEKMK
                     NQDIPREELIKIFEGAGLKSEFKEDEDVLSVASKDIEERINNHKNIRFVGLQFLSLLD
                     FMWMNHLENIEALMESVRLRAYAQKDPLVEYRRESFMFFEALRNNIEMWLLNNVLKLE
                     NVEDKKGTVSTSAPKDIKISVKDKVGRNDPCPCGSGKKYKKCHGK"
     gene            complement(6953..7276)
                     /gene="raiA"
                     /locus_tag="VI432_02715"
     CDS             complement(6953..7276)
                     /gene="raiA"
                     /locus_tag="VI432_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY4497037.1"
                     /translation="MKIDIITKNLELTAPLREYIEIKIGSLEKFLEKFKEMDLDIKIE
                     VARTTKHHKQGEVFYAEGNIALPGSVLVASSESWDIRVAIDDIKNKLQREIRKYKGKA
                     DPTNQ"
     gene            complement(7518..7688)
                     /gene="rpmG"
                     /locus_tag="VI432_02720"
     CDS             complement(7518..7688)
                     /gene="rpmG"
                     /locus_tag="VI432_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY4497038.1"
                     /translation="MAKKFSEHLIRLKCSKCKRANYYTRKNKKVVERKIELSKFCSWC
                     RKRTTHKEAKVK"
     gene            complement(7741..7813)
                     /locus_tag="VI432_02725"
     tRNA            complement(7741..7813)
                     /locus_tag="VI432_02725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7778..7780),aa:Thr,seq:ggt)
     gene            complement(7866..7936)
                     /locus_tag="VI432_02730"
     tRNA            complement(7866..7936)
                     /locus_tag="VI432_02730"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7902..7904),aa:Thr,seq:tgt)
     gene            complement(8043..>8397)
                     /locus_tag="VI432_02735"
     CDS             complement(8043..>8397)
                     /locus_tag="VI432_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497039.1"
                     /translation="VSFANHAVKPAKKVARLLSIDRPMTTDEIVEGMEQIGWSEGTLR
                     ELVAVLVARPHLRERVVAFGSRAFIQGKWTTPCLVFEDGRLKIISSEPKTKWSSSWRF
                     LAVRPTRERASRRKV"
BASE COUNT         2492 a         1985 c         1279 g         2641 t
ORIGIN      
        1 ctgccgtcat cgctaaatag catatctgct ccgtcttctt ttaaaatcgc gatttcggta
       61 ttcaaagaag ccacaacctc agcaacgtct tttattaacc ctagcgctcc aggcgcctca
      121 ttaagaaacg gcacaatttc aagaggtttt atacgatagc cgattttatc tatctcttct
      181 ttaattaaat ctataaatcc agcggtttta tccgtttcta aatccaataa actttctgca
      241 gctaatttaa aactgtcgta tattttcagt ccggaactaa aagcgtcatc tttataattc
      301 catagataaa aagcataaga agaaacagct aaaacgccta tgattaaaat gcttaaaatc
      361 aaaactttca atatcttaaa tctgtctcca aattccaatt taggccttga atatatatcg
      421 ccctcgctaa aatacgggct tgcggttgaa aatctagaac tcccccttgg aggacgtata
      481 tctataacca cattttttct aggtcttctc tttttattca tatgtctttt aatcataccg
      541 cattaggatc agaaaataat atagaaagtg ttttaaaaag tattttgacg tctatccata
      601 aagacacatg atcaatgtaa aacttatcca actcaacctc ttttaaaaag ggtagactcg
      661 acgctccatt cacctgagaa aggccggtta ctccggcgcg cgacttcgcg atcagcttat
      721 attcattagg atatttttct atctcctctt cttcgtgagg acgcggaccg accagcgaaa
      781 gctctccctt taaaacatta attaattgtg gaaattcatc tatcctgaat tttcttaaaa
      841 tcctgcctac ctttgtcact cttgggtcat tagctatttt aaaaaacggg ccgtcatttc
      901 tttcatttaa atgtcttaat ccgtctttca ttttatcggc ttcaggaacc atatttctaa
      961 atttataaac ataaataatt ctaccctcgc ttattcttgg caaccttacg ataaatgacg
     1021 cctcgggatg ctcaagctta atagcaagta ttatgaaagg cgctaaaggc aatatcaaaa
     1081 gcatcccagc taacgcgccg agtatatctt caattcgttt ccaaagagga tagttcactg
     1141 taaaattata tagtaatatt gcaacattag aatgcccact atatttaata aaaaaccgcg
     1201 cgttgccttg ttacacgatt accttaatca atatggagga gccgaaaggg tgcttgaaac
     1261 atttatagaa ttatttccgg atgcgccaat ctacactctt ttttatgata aagaattaat
     1321 gggtggtcga tttaataata aaataattaa aaccagtttt ctcgacaaaa aaatcatccg
     1381 ccgaaatcat agatttttta tccccctgat gcctaaagcg gccgagagca ttgacttggg
     1441 agataactat gactttataa tttctgattc tgcgggatgg tctaaaggta taaattataa
     1501 aaacgcagtg catattaact atttgcactg ccctctccga tacgcatggg aaccaaaaag
     1561 atatcttgat actcttatac ccgaatattt aataaaatcc gccggaccaa tcatcaaata
     1621 tctccgcgat tgggacaaag aaacgggctt aaaacccgat attttaataa ctccttctaa
     1681 atttacagca gaaaaagtag gtagatttta taatagagat acaaaaattt tgcacccacc
     1741 gatagacaca gacatatttt tttacgaacc ttccatagag gaaaaaatcg aaccatactt
     1801 cttaacattt ggcaggatag tccatcaaaa aagatttgat ttattggtga gcgcattcaa
     1861 caagcttgat gtgaaattaa aaattgtcgg ctctggatta gacatcaaaa atatagaaag
     1921 aataaaatct tctccaggga tagatttatt aaactgggtt gaagataatg aattaagaaa
     1981 attaatcagt ggggccaagg cgactatatt cgctcagata gaagactttg gaatggtggc
     2041 cgcagaatcc atagcatgcg gtacgccagt catagctttt aacgaaggtg gatttaaaga
     2101 gatcattaaa gagggcgaaa acggcatatt ctttaacgag cagaaagaag aatctattat
     2161 agatgcgctt aaaaaattcg aaaaaacaaa attcgataga gaagccgtag cctcctcttc
     2221 aaaacaattt cataagaaaa cctttatcaa agacttcaaa aaaatattat ctgacgcggg
     2281 tctatatatt taattaaata ccaagatacc taaagcgagg cgcgctcttg cccccgataa
     2341 caacatcctc cataaacata tcaagcggcc ccacccatat atgtccttga tctaaagatt
     2401 gataaacaac cagctctctt tccttgtcct cgctatctct tgcaagacag atcacttcat
     2461 attgcccgcc tttaaaatgc tcgtactttc ctaatttaaa actgcgagct ctctctgata
     2521 tcggaactaa cttatattta tcggagttta ttttttccat tctacttttc tgtcattata
     2581 tataatgagc ctcaactagc tcctcataaa ccattattca attgagatta tctgcaagcc
     2641 cttttcaata atctcatctt ccggccacca tggagaatcg tcaattctga attttgatac
     2701 atgagtcact ttcttctcta cgctcctccc tgtatattct ttagtcttcg gatcccattc
     2761 ttccagaatc agagtgtcgc cctcttttat ttcaaactct tgaagacgca tatcatactt
     2821 tctcttaccc gaggccattg agtcaaaata ttccggccaa atttttttct tgattattgc
     2881 catactgcta ttatatcaga tgttttaaac aaaataatat atcttatata tcgcatatat
     2941 cacgcctacc aagtacgcag cttcatcccc cttttatccc catggggata aaagggggat
     3001 gaaataaaaa gcattataga caaaaagaat tgacagcagg gtaacgctat tatatgaata
     3061 aagtgctctt ttccaaatag gaacgatcca aatgatgaga gaaacaacat ctcctgaaga
     3121 agccaaagag gcaaggaggc gacgtggcag agctataatt cttatagaga aacttaggaa
     3181 tcaaccaggt acatacttta cactaggtca gatcacctgg tttactcgcc tgaccgatcc
     3241 gagcaagccc ttcaatccta ttgaagaaga tcttgttaaa ctccaagaga tgacagatca
     3301 agccagtcaa gacacggaag ccttctccaa ggtagccagt ggacgcgcac gatgcacgta
     3361 agatacccat acataccgac cgatactggt cggtcttata ttttcattaa tcactaaacg
     3421 ctctctcgtt attgagctat ttcgctttct tgctaacgat ctatcccgct attaactatt
     3481 cttagtacat tctttcaccc aacgacacat ctttatcagg agaaagtaat acagcgtttc
     3541 catctctatc tgtcagacca agcgtaataa attcagaggt gaatggtcct attttttttg
     3601 gcacaagatt aatacagcct attatcaatc tgcccattaa ttcatctttt gtataattct
     3661 tggtaaactg cccagcagat atcttaatcc caacctccgg accaaaatca accgtaaatt
     3721 tatacatcag cttcctcgct tccggaaaat cttcaacttt aataatcttc cctactctta
     3781 tatcaacctt ctcaaaatcg ctgtattgta tctcgttatt ttccatatct tttatctatt
     3841 ttcttcattt ttttaattaa agctcgatca atatcaatat ccatagcttc tgccaaaagt
     3901 agagtcgtta ttaaaacatc tgcgaattcc tcggataaat tatcaccgct cttcttcatc
     3961 ttgcccttcc tctgcatggc attatgtctt aaaaccgcat cacacaactc tcccaactct
     4021 tcagaaagtt ttacggttct cgcgagtatc cgcttctctt catctttata attaccgtaa
     4081 taccttctta acctcttatc ttccttcttt ataaaccgca tcaaatcttc aaactgcata
     4141 tctttatttc ccattgttat tttttatcca ccacttctta aaaaatggca tcgttaaagt
     4201 cgatagcata aacgctacgg cagggataac agcattcaac catggctttg gcacgctaat
     4261 ggcccacata tatgtaccaa tcgccagata agtgaatgcc attaatagaa aacgcctagt
     4321 ccaacttgtc tcccacgcct tatccacttc gactctttta ttcctatttt caattacttg
     4381 tatcttacgc tctatatcct tcatattact ttccatgaca cttcttatat ttcttcccgc
     4441 tgccgcatgg acaaggatca tttctgccga ccttatcctt aacgcttatc ttaatatctt
     4501 taggcgcgct tgtagagact gttccttttt tatcttcaac attttccaat tttaaaacat
     4561 tgttcagaag ccacatctca atattattcc tcaacgcctc gaaaaacata aaactttctc
     4621 ttctatattc aaccaacgga tctttttggg cataagctct cagccgaact gactccatca
     4681 acgcctcaat attttccaaa tgattcatcc acataaaatc taaaagagac aaaaattgaa
     4741 gtccgacaaa cctaatattt ttatgattat ttattctttc ttctatgtct tttgaggcaa
     4801 cactcaaaac atcttcgtct tctttgaatt cagattttaa tcctgcgcct tcaaaaatct
     4861 ttatcaattc ctcgcgagga atatcttggt ttttcatctt ctcgaattga gaatctacat
     4921 accgcctgac aatcgtttta atttcaacct cgttttctaa cgaatctaaa acttctgccc
     4981 taatttcata aaattttaat ctctgcttat ttaaaatatc atcatattca agaaggtgct
     5041 tcctcatatc aaaattgaaa ccctcaacct tactctgcgc ctgctgaatc gagccggaga
     5101 ccattttact cgccggcagg gcatcaggca tattaaattt atccattaga cctttaactc
     5161 tgtctccgcc aaaaattctt gcaatatcat cctctaaaga aacgaaaaac tgagacgatc
     5221 ccggatcgcc ttgcctgcct gcccttccgc ggagctgatt atcaattctt cgagcctcgt
     5281 gcctctcagt gcctatcacg tgcaaaccgc caagatccct aaccttccta gcctcttcgg
     5341 aatcgggcgg attaccgcct aaaatgatat cgactccccg acctgccata ttcgttgcga
     5401 cggtcactga tccggatttg cccgcttggg cgataatcga tccttctctc tcatgatttt
     5461 tagcatttaa aacctcgcaa ttaatgccct ctcttttcag aatatttccg agaagctcat
     5521 tcttctcaat ggaaacagtt cctatcaaaa tcggctggcc ttttttattc ctatccctga
     5581 tctcttcgac tattgcttta tattttgctt cctgcgttct aaaaataaca tccggcagat
     5641 ccgccctgac caaaggctta ttagtaggaa cgcttaccac ctcaagtcca tagactttat
     5701 gaaattcctc ggccgatgtc tgcgcagtac ctgtcattcc ggaaatcttt ttatataatc
     5761 tgaaatagtt ttgaattgtt atttgagcgt aagtcttcga ctcttctttg accagcactc
     5821 cctctttagc ttcgatagcc tgatgcaatc cgctagaata tcttctaccc aacatcaatc
     5881 gaccagtaaa ttgatccact aagataactt ctcctgaacg aacaacataa tccttatctc
     5941 tatcgaaaag cgctctcgct ttcaaactag cctcaagata atgaaccaat cgtaaattct
     6001 ccggcgcata aatatctttt atgccaagca tcccttcaat cttatctatg ccggaattgt
     6061 ttatctcaac tgtttttaat ttctcatcaa ccgcataatc ttcatcttgt ttcaaatttc
     6121 taaccgctct tgcgaatacc ttataaaact ctgaggattc tttatctggc acagatatga
     6181 taagaggagt cctcgcctca tcaatcaaaa tagaatcaac ctcatcaata atcgcaaaat
     6241 tcaattctct ctggactttc atttctttac tctgcaccaa attgtctctt aaataatcaa
     6301 aaccaaactc gtggtttgtg ccataagtta tatccgccga ataagcctcc ttgcgagaaa
     6361 ccggcctcaa atattcttcg tgtactaaaa aaccgccttt ttcatctcta tccttatcaa
     6421 tttctttttt ctcatcgctt gacgccctcc actccggatc ataaatataa gcggaattat
     6481 gcgctatgca tcccgtttta agacccaata aattataaac ttgccccatc cagacagcgt
     6541 cacgtttggc cagataatca ttaacagtaa ttagatgaac ccctttaccg ctaagagcgt
     6601 ttaaataagt cggggcagtt gcagccagag tctttccctc tccagttgac atctcggcaa
     6661 tagcgccttg atgcaaaaca attccgcccc aaaattgcac atcaaaatgc cgttggctca
     6721 aggctctttt cgcggcctcc ctaaccaaag caaaagctct aggcaaaaat tcatttaagt
     6781 cggctccact aaaaatagct tctttgattt tcaagctttc agccctcaaa ccagcatcat
     6841 caagaatctc aatctctttt tctaaattat taataagctc taactcttta ggcggacgcg
     6901 ggactgaact aaaaatcttc tttattaagc tggccatcaa gatctctata ggctactgat
     6961 ttgttggatc agcctttcct ttatactttc taatctcacg ctgtaattta tttttgatat
     7021 catcaatcgc gactctaata tcccaagact cgctggaagc gaccaagacg ctgccaggca
     7081 aagctatgtt gccttcagca taaaacactt ctccctgctt atgatgtttt gtagttcgag
     7141 ccacttcaat tttaatatcc aaatccatct ctttaaattt ctctaagaat ttttccaagc
     7201 ttcctatctt aatttctata tactctctaa gtggcgcagt taattctaaa ttttttgtga
     7261 ttatatctat cttcatctcg ttaaaattat agcagaatta atatgattat tctccagtta
     7321 ttattatcga cttatattat ttaattagtg tttattctaa tttttacccg ttaggatgcg
     7381 tctcactggg cgaagtatcc catcatgagc ggcggcggtg acctcctatt atttgttatt
     7441 ttttcaagtg agtgctcaac cgccgctcac agatattgta tgttcaagcg cgccaatgag
     7501 tcaacgcgta ataaaaccta tttaacctta gcctctttat gagtagtgcg ttttctgcac
     7561 caagaacaaa acttactaag ctcaatcttg cgctcaacaa cctttttatt cttgcgagta
     7621 taataattag cccttttgca cttagagcat tttaagcgaa ttaggtgttc tgaaaatttc
     7681 tttgccatat ttaagtgaaa aaatagtaaa ctatttcctt ataattatca aatttatctg
     7741 agccaatggc cggaatcgaa ccggcgacct actccttacc atggagttgc tctgccgact
     7801 gagctacatt ggcaatgtca aaagaacttt ttgagtttaa agtaagttaa ccgtgttatc
     7861 aagtgagcct cctacaggaa tcgaacccgt atatgtgctt tacaaaagca ctgttctgcc
     7921 attgaactaa ggaggcaaag cttaatctat cataaactag attttctaat ttagaaaagt
     7981 ctaataaaaa agatcgcctc gtgcaaatac atgcacgagg ctcttcgtaa aagaacttcg
     8041 ttctacacct ttcgccggct tgcccgctct cttgtcggtc ggaccgcgag gaaacgccag
     8101 ctagagctcc acttggtttt cggctcgcta gagatgatct tgagcctgcc atcttcgaag
     8161 accaaacatg gagtggtcca cttgccttga ataaaggcgc gtgagccaaa agctacgact
     8221 ctctctcgca aatgaggtct cgcgacaagc accgccacaa gctcacgcaa ggttccctcg
     8281 ctccagccga tttgctccat cccttctacg atctcatccg ttgtcatagg acgatcgata
     8341 gagaggagtc tcgccacctt tttcgcgggt ttcaccgcgt ggttggcaaa actcacg
//
LOCUS       DATDNZ010000070         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3372703, whole genome shotgun sequence.
ACCESSION   DATDNZ010000070 DATDNZ010000000
VERSION     DATDNZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372703"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2254)
                     /locus_tag="VI432_02740"
     CDS             complement(<1..>2254)
                     /locus_tag="VI432_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY4497040.1"
                     /translation="QSKKGLVEQADGGTLFLDEIQNLPPSAQAILLRVLDTNEYLPIG
                     ESTPRYSDFRLITANNRGSLHEQVKEGKFDDSLYYRINGLELWLPPLRKRRKDIPLLV
                     EHFQKLLAVEHQVEIKPLSSATMQVLMQNNWPGNIRELFNVVKRIFILGEALGIPAND
                     QRVEGKHKGGSIDIKVSNDKKEERIVGTSLSLKHAVAVADQVAPSHVPVLISGDTGTG
                     KSILARYIHEISPRKDGPFVIINLTQVPVALFESEVFGHMKGSFTGAVDNKPGLIETA
                     HGGTLVLEEITSIDFLQQAKLLRVLQDKRFRRIGETQERYSDFRLITLVNGTRLEQLM
                     KQSEFREDLLYRIGVIRIELDQLRNRKQDIPLLIDYFLQRFYTKLGIEVRPLSDELRE
                     TLLNYQWPGNIRQLASFIRRIVSFGEMGEKMIIEEIKSDSSSSRKKSSSPVSRDENKT
                     HALYQSVLGFVSHYDVWRKSHPDSKLIFIVRHGESKTNLFEEQQTYETFSPLTLKGRR
                     QRREFKKFIQKHLGNVIDRYIASDLERAYETIRLLARSEKKKPEILRRFREALIVPFF
                     EELNRFIDDPENFEFGEYAGRKVKLYLRKFIQEFINGDDKNIMVASHGITLLLMIMEI
                     LDIPYKTYSQIYRKLGNAPHLGITIFSYEPHKNIWQLLVYGDDSYLSKETRGKTTRTF
                     EIKLNRFRFILLGFWRLMLKRIGFRNSYLRDYYPSRKRPANNRASSPVRQERTERLTT
                     LIHSLGLLRL"
BASE COUNT          615 a          502 c          407 g          730 t
ORIGIN      
        1 attaatctca acagccccaa ggaatgaatg agcgtggtaa gccgctcggt tctttcttgc
       61 ctgaccggcg aggaggcgcg gttatttgct ggacgttttc gggagggata ataatctctt
      121 aagtaactgt ttctgaaacc aattcgcttg agcattagcc tccaaaaacc aagtaaaata
      181 aatctaaaac ggttaagttt aatttcaaac gttcgagtag tttttcctct tgtctctttg
      241 ctcaaatagg agtcatcccc atagactaat aattgccaaa tatttttatg aggctcgtaa
      301 gaaaaaatag taattccaag atgcggtgca ttgcctaatt tcctataaat ttgagaatat
      361 gtcttatagg ggatatccaa aatttccatt atcatcagca gtaaggtgat tccatggctt
      421 gccaccataa tgtttttatc gtcaccatta ataaattctt gaataaattt tctcaagtat
      481 agttttactt ttctccccgc atattctcca aactcaaaat tctctggatc atcaataaac
      541 cgattaagct cttcaaagaa tggcacaatt aacgcttctc gaaatcttct taaaatctca
      601 ggctttttct tttcacttcg cgctaaaagc cgtattgtct cataggctcg ctcaagatcg
      661 cttgcaatat aacgatcaat gacattcccc aggtgttttt ggataaactt tttaaattct
      721 ctgcgctgtc gacgaccttt caatgtcaac ggagaaaatg tctcgtaggt ttgctgctcc
      781 tcaaaaagat tagtttttga ttcgccatgc cgcacaataa agattaattt tgaatcagga
      841 tgagattttc tccatacatc ataatggctg acaaatccca atacactctg gtacaatgcg
      901 tgtgttttgt tttcatctct acttaccggc gaggaagatt ttttcctgga agaagagctg
      961 tctgatttta tctcttcaat gatcattttt tcacccattt caccgaatga aacaatccgc
     1021 cgtataaatg aggcgagctg tcggatattg cccggccatt ggtagttcaa taacgtttct
     1081 ctcaattcat cgctaagtgg ccttacttca attccgagct ttgtataaaa tctttgtaag
     1141 aaataatcaa tgagcagggg aatgtcctgt tttctatttc tgagttgatc gagctcgatc
     1201 ctgataactc cgatacgata aagaaggtct tccctaaatt cactttgttt cattagttgt
     1261 tctaagcgtg taccattgac caatgtgatc agccggaaat cagaatatct ttcttgtgtt
     1321 tcgcctatcc gtctgaatct tttatcttgc aataccctca atagttttgc ctgctggagg
     1381 aaatctatag atgttatctc ttctagaaca agcgtaccgc cgtgagctgt ttcgataagc
     1441 ccgggtttat tgtcaacagc cccggtaaaa gagcctttca tgtggccaaa tacttccgat
     1501 tcaaacagcg ccaccggtac ctgcgtaaga ttaataataa caaacgggcc gtcttttcgc
     1561 gggctgatct catggatata ccgcgctaat atactttttc ctgttcctgt atcgccgctg
     1621 attaaaactg gcacatgtga aggggcaact tgatccgcta ctgccacagc atgctttaat
     1681 gacaaagagg tcccaacaat tctttcttct tttttatcat tactaacctt tatgtctatc
     1741 gatccccctt tatgcttccc ttcaactcgc tgatcatttg ccggaatgcc aagcgcctct
     1801 ccaagaataa atatcctctt taccacgttg aataattcac ggatattccc cggccaatta
     1861 ttctgcatca gtacctgcat agtcgccgag cttaaaggct ttatttcaac ttggtgttcc
     1921 acggccaaga gtttttgaaa atgttcaaca agaagcggaa tatcttttct tctttttctt
     1981 agaggaggaa gccataattc caaaccatta atacgataat aaaggctgtc atcaaatttt
     2041 ccctctttaa cctgctcgtg aagagagcct ctgttattcg ctgttattag ccggaaatcc
     2101 gagtaacggg gcgtcgattc tcctataggc agatattcat tagtatctaa aactcttaat
     2161 agaatggctt gcgcgctagg cggcaagttt tgaatttcat ctaaaaatag cgttcccccg
     2221 tcagcttgct cgacaaggcc ttttttagat tgat
//
LOCUS       DATDNZ010000071         7582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2835254, whole genome shotgun sequence.
ACCESSION   DATDNZ010000071 DATDNZ010000000
VERSION     DATDNZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835254"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VI432_02745"
     CDS             <1..598
                     /locus_tag="VI432_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY4497041.1"
                     /translation="IITIFLSSYMIIHGSRLYNFLKPILKFFEFRRDTIEETGEETLL
                     EDHIILVGAHRMGHNILRALDKSGESFVAVDFDPRIINKLRENNLPCFYGDITDFEIQ
                     KLVGLNKARLVISTVPDIKDNRSILSFIKNKNPGAKIILTAESEHEAELLYSEGADYV
                     LLPHFVGGVELAEIIRVDKNLTDLVRLKERDLALIRAS"
     gene            980..3478
                     /locus_tag="VI432_02750"
     CDS             980..3478
                     /locus_tag="VI432_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY4497042.1"
                     /translation="MPNKSVVYFKNYLLDMRPLTRAFIRMTIGIWMIVLTILTLVLLL
                     SDIEPLYWSGTIVLLYLIDRLIHRNDGDRRISRMPPTGRINIAEYMTPASLKAIIGAH
                     DKTYLENGNILLYLTSILIDDRGVREAFLRLGIKEDEFRVKLDEYLNVHKVEERESEE
                     VVSRNISIISIKALEQAIAGQDEYIDPVDLFGALGFMENKDLSRIFELFNIKPGDVEK
                     SLIFGRVKKRGSWISGLPKTVGGFARRHMRHRVMNRAWTSRPTPNLDRFSADLTDVAR
                     SGGLGFMIGHEDEYNRLLNILTRPVKPNALLVGEPGIGKEVIVGHLAYMITKDKVPEQ
                     LFDKRLVALDVASIVAGAEQSEMQERIKIIFDEIYKAGNIILYIPEVHNLSRTSGAKQ
                     ISAANAIVPLIVSNNFPLIGTTYPREYKVFIEQDSAFSSAFDIIRVKESTLDEAEKVL
                     AYESVLFEKQFRIKITFEAIKTAVHLANKYFHNRPLPGSASDLLKEAISAEARSKGEL
                     VDGEDIVRVAEARVNIPIKKAGREEVEKLLNLEELIHKELINQENAVKLVAESLREYR
                     SGLSRKGGPIGSFLFVGPTGVGKTELSKILTKLQFGSESAMVRFDMSEYQDKQSFFQL
                     IGSPDGTVTGALTERVIEKPYSLILLDEFEKAHPDILNLFLQVFDDGRLTDNLGRVVN
                     FENTIIIATSNAHSDFIKNEIESGRDMVEISGELKKKLVTFFKPELLNRFSAIVVFTS
                     LNRDHIRQITKLQLDSLIKKLKDEQGIYISITDALINKIAEWGYDPVFGARPLRQVIS
                     SKIKTPLSKTILNQDSVRGLSLSMDLDGEEVKIG"
     gene            3489..5258
                     /gene="polX"
                     /locus_tag="VI432_02755"
     CDS             3489..5258
                     /gene="polX"
                     /locus_tag="VI432_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY4497043.1"
                     /translation="MKNGEIAKILLDIGEYLKMQEEPFRPKAYEKAGRVISDLEISVH
                     DLYRDGGVKALGKISGVGVSIAEKIEEFLKTGRIKYYEDLKKKTPVDLETLTRVEGLG
                     PQKIKVLYYKLKIRNIRDLEKAASSGRIAKLKGFGRKSEENILKGIEFIKKSGKRFTL
                     GEVLPTAKDIEGRLSRVSGIEKIVIAGSVRRRKETIGDIDILAVSKKPEAIVSVFISM
                     DEVGMVIAEGKTKASVKLNNGLDVDLRIVDRRSYGAALNYFTGSKEHNIKLRNIAIKK
                     GYKLNEYGLFSAKKTLGSGEKSWRKIAGENEDGLYKKLGLNYIPPEIRENEGEIELSS
                     QDKLPKLIGYEDLKGDLQIQTSWTDGADSIELMARQAIKMGLEYIAVTDHTKRLAMTN
                     GLNERRIREQIREIEKLNLKFKTQNLKFRILKGTECDILKDGSLDLPDGILSKLDIVG
                     ASIHSYFNLSKRDQTDRLKKAMSNKNVDIIFHPTGRLINKRPAYEIDIEEIIEFAEKT
                     KTVLEINSFPDRLDLRDEQIRMCVQKGVKMSIDSDAHSMGHMKYLEYGIAQARRGWAK
                     KGDIINAWPIDKMLAFLKENKSK"
     gene            5260..5715
                     /locus_tag="VI432_02760"
     CDS             5260..5715
                     /locus_tag="VI432_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4497044.1"
                     /translation="MTKEISAGLIVYRRTKGGIKFLLLYHGGKYWNFPKGHIEAEEKS
                     METAIRETTEETGLRRVDLKIKNRFKGYEKFTFFRNKTKIFKIVIFYMAETNNRQIRI
                     SKEHDGFAWFTYKEAYNILKKYRDSINVLNKANEFIVLGDKSIKPTPAS"
     gene            complement(5712..6059)
                     /gene="rplT"
                     /locus_tag="VI432_02765"
     CDS             complement(5712..6059)
                     /gene="rplT"
                     /locus_tag="VI432_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY4497045.1"
                     /translation="MTRVKRGVLNKKKRSKTLKYTKGFGWRRKTHERAAKEALLHAWT
                     NAFRGRKQKKRDYRTLWTSKINSEVRNENLTYSKFINALKKKGIKIDRKILADLAENN
                     PAVFKKILSEATS"
     gene            complement(6062..6241)
                     /locus_tag="VI432_02770"
     CDS             complement(6062..6241)
                     /locus_tag="VI432_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018931299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY4497046.1"
                     /translation="MKKSVTKRIRKTRTGKMMRRTMAQDHFRAGKPQKYLRNKRKSAK
                     ISAQDYKRIIKYQNR"
     gene            complement(6238..6765)
                     /gene="infC"
                     /locus_tag="VI432_02775"
     CDS             complement(6238..6765)
                     /gene="infC"
                     /locus_tag="VI432_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY4497047.1"
                     /translation="MILLIQQEPKINNQITAKELRVIDEGEGNLGVLSLQDALAKAKA
                     KELDLILIVPSATPPIAKIISYDKFRYQRAKELKKQKQAQKVPEMKQIQISAREAKND
                     LSIKSKRIDKFLEGGHRIEVRMRLRGREKQMKDWAKEKINEFMKLITHPHKTTQEIKF
                     FGGVFSTQIDPIKQQ"
     gene            complement(6893..>7582)
                     /locus_tag="VI432_02780"
     CDS             complement(6893..>7582)
                     /locus_tag="VI432_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497048.1"
                     /translation="ILPPVYFSSEAMLLNPDWIGSEALYRHPAVQAALEATEYWAWFI
                     GKNAPEFPHLANLSWETKVLGYDAGYAEFMDADIIIDIAMVSDPHAGSQHAGLASGNY
                     NHVSGKQYCLATNAGIGAMQESDQPTDLRNIVLHEFGHCIGAGHTGSFPPNEQCSPTY
                     GCFDNQPEDVMTGNSHYKRNCLSNINLFSLAEIHDSLPPIQNAGWYDGEAFVLKEDYG
                     QTCMPEAMNRF"
BASE COUNT         2294 a         1248 c         1660 g         2380 t
ORIGIN      
        1 aataataact atattccttt cttcatatat gattattcat ggaagccggt tatataattt
       61 tcttaagcct attctgaaat tttttgaatt caggagagat actatagaag aaaccggaga
      121 agaaacgctt ttggaagatc acattatctt agttggagct catagaatgg gacataatat
      181 tttaagggcg cttgataaat ccggagaatc tttcgtcgct gttgattttg atccaagaat
      241 tataaacaag ctaagagaaa ataatcttcc ttgtttttat ggcgacataa ctgatttcga
      301 aatacaaaag ctagtaggat taaacaaagc aagattagtt atttcaacgg ttccggacat
      361 taaagacaac agatcaattt tatcttttat taaaaataaa aatccaggcg ctaaaatcat
      421 tcttacggct gaatcggaac atgaagcgga gttattatat agtgaagggg cagactacgt
      481 tctgttgcct catttcgtgg gcggcgtaga gcttgctgag attatacgcg tcgacaaaaa
      541 tttgaccgat ctagttcgcc ttaaagagag ggatctcgct cttataagag cgagctgatg
      601 ggcgggcata cgcgcttata agcagttata atataaataa tgatatcttc gctaatttat
      661 cttgctaata gatttgttta cgggattata gattttttaa accactggta tataggtagt
      721 tttttggttg tgagcgattt tatgcttaat ttattcgaac agttagatta tgtttttgct
      781 ctgagggtca cgttaaaaaa ttttttcaaa ccactatatc aagataaaac cattatcggt
      841 tatattttgg gatttttcct gcgtactggg agagttttgg tcgcgctcgt tgtttatata
      901 tcggttttta ttttatccat agttatttat gtgttttggt tatcaataat tccatatata
      961 gtctataaaa ttttttcata tgcctaataa atctgttgta tattttaaaa attatttgct
     1021 tgatatgcgt ccattgacca gagcgtttat cagaatgaca atcggtatat ggatgattgt
     1081 tttaacaata cttactttag ttcttttact ttctgatatt gagcctcttt attggtctgg
     1141 gactatcgtt cttttatatc ttattgatcg attgatccat cgtaatgacg gagatagaag
     1201 aatttcgcga atgcctccaa cggggcggat aaatatagct gagtatatga ccccagcaag
     1261 cctcaaggca ataataggtg ctcatgataa aacttatttg gaaaacggca acatccttct
     1321 ttatttaaca agtattctta tagatgatcg cggggtgaga gaggcctttt taagacttgg
     1381 cataaaagaa gatgagttta gggttaaact cgacgagtat ttgaacgttc ataaagttga
     1441 agagagagag tcggaagagg ttgtgtcccg aaacatctct attataagta ttaaggcttt
     1501 agaacaggct attgccggcc aagatgaata tattgaccca gtggatttat ttggagctct
     1561 tggatttatg gaaaataaag atttatcccg aattttcgag ctgtttaata tcaagcccgg
     1621 agacgttgag aaatctctta ttttcggaag ggttaaaaag agagggtctt ggataagcgg
     1681 tttacctaaa actgttggag gttttgctag gcgtcatatg cggcacagag tgatgaatag
     1741 agcttggaca tcaagaccaa cacctaatct agacagattt agcgccgatt taactgacgt
     1801 tgcaaggtct ggcggattgg gttttatgat cgggcacgaa gatgagtata ataggctgct
     1861 taatatttta acaaggcccg tgaagccaaa cgccctttta gttggggagc cgggcatagg
     1921 caaggaagtt atagttggac atttagctta tatgataact aaagataaag ttccagaaca
     1981 gttgtttgat aaaagattgg ttgcgcttga tgtcgcttct attgtcgccg gagcagagca
     2041 atctgaaatg caggaaagaa taaaaattat ttttgacgaa atttataaag ctggcaacat
     2101 tattttgtat attccggaag tgcataattt atccaggact tcaggagcaa aacagattag
     2161 cgcggcaaat gccatagttc cccttatagt gtctaataat tttcctctaa taggaacaac
     2221 atatccaaga gagtataaag tttttattga gcaggacagc gctttttcaa gtgcatttga
     2281 tatcatcaga gttaaggaat ctactcttga cgaagccgag aaagtgcttg cttatgaaag
     2341 cgttttattt gagaagcaat tcagaatcaa aataactttt gaggctatca aaacagctgt
     2401 ccacctcgca aataaatact ttcataaccg gcctttgccc ggatctgcga gcgatttatt
     2461 aaaggaggcg atatcagcag aggcgagatc taagggcgag ctcgttgatg gcgaggatat
     2521 tgttcgtgtt gccgaagcga gagtgaatat tccgataaaa aaagctggtc gcgaagaagt
     2581 ggaaaaatta ttgaatttgg aagaattaat tcataaagaa ttgattaatc aggaaaacgc
     2641 agttaagctc gtggccgaat cattaagaga gtatagaagc ggactttcgc gtaaaggcgg
     2701 tcctatcgga tcttttcttt tcgtgggtcc tactggggta ggaaagactg agttgtctaa
     2761 aattctcacc aaattacagt ttggatccga gagcgcgatg gttcgtttcg atatgtccga
     2821 atatcaagac aaacaaagct tctttcagct aatcggatct ccggatggaa cagtaacggg
     2881 agcgcttaca gaaagagtga tagaaaagcc ctatagttta attttgcttg atgagtttga
     2941 aaaagctcac ccagatattc taaatctttt tctgcaggtt tttgacgatg gaaggttgac
     3001 tgataatctg ggacgggtag ttaattttga aaacacaata ataattgcca cttctaatgc
     3061 tcattcagat tttattaaaa acgagattga atctggcaga gatatggtgg aaataagcgg
     3121 agagttaaag aaaaaattag taacattttt caagccggaa ttattaaaca gattctccgc
     3181 gattgttgtt tttacaagtt taaacagaga tcatattaga caaattacca aactacaact
     3241 tgatagtctt attaaaaaat taaaagatga gcaaggtata tatatttcta taacagatgc
     3301 gctcataaat aaaatagcgg aatggggtta tgatcctgtt ttcggcgcgc ggccattgag
     3361 acaagttata tcatcaaaaa tcaaaacacc cctttctaaa acaatattaa atcaagactc
     3421 tgtaaggggc ttatcgctct ccatggattt agatggagaa gaagttaaaa taggctgaga
     3481 atagtttcat gaagaacgga gagatagcaa aaattttatt agatattgga gaatacttga
     3541 agatgcagga agaacccttc cgtcctaaag catatgagaa ggcgggaagg gttatctcag
     3601 acttggagat cagcgttcat gatttataca gagatggtgg cgtgaaggcc ttaggtaaga
     3661 tctctggagt tggcgtttca attgcagaga aaattgagga gtttttgaag actggcagga
     3721 taaaatatta tgaagatttg aagaaaaaaa caccggttga tttggaaacg ttgactagag
     3781 ttgagggttt gggtccacaa aaaataaagg ttctttatta caagcttaaa atcagaaata
     3841 tcagagatct ggaaaaagct gctagctcag gcaggattgc caagttgaaa ggtttcggca
     3901 gaaaatcgga agagaatata ttaaagggta tagaattcat aaaaaaatct ggtaagaggt
     3961 ttactcttgg ggaagtttta ccgacagcta aagatattga aggcagatta agtagggtta
     4021 gtggaattga gaaaattgtt atcgcgggat ccgttagaag acgtaaagag accattggtg
     4081 atattgatat tctggctgtt tctaaaaagc cagaagctat tgtgagcgta tttatttcta
     4141 tggatgaggt aggaatggtt atcgccgaag gcaaaacaaa ggcatcggta aagcttaata
     4201 atggtttgga tgttgatcta agaatcgttg atagacgatc ttatggggca gcccttaatt
     4261 attttaccgg ttccaaagag cacaatataa aacttcgcaa tatagcgatt aaaaaaggtt
     4321 ataagttgaa tgaatacgga ttgttttctg ctaaaaaaac actcggctcc ggagagaaaa
     4381 gctggcgaaa aattgccgga gaaaacgaag acgggcttta taagaagttg ggattgaatt
     4441 atatcccgcc cgagattaga gagaatgagg gtgagatcga gttatcaagc caagataagc
     4501 tacccaagct tattggctat gaagatttaa aaggagactt gcagatacaa accagttgga
     4561 ccgatggcgc agattcaatt gagctgatgg ctagacaggc gattaaaatg ggattggagt
     4621 atattgctgt aactgatcac accaagaggc ttgctatgac aaacggccta aatgagaggc
     4681 gcataagaga acaaattaga gagatagaaa aacttaatct aaaatttaaa actcaaaatt
     4741 taaaattcag gatattgaag gggactgagt gtgatatttt aaaagacggt agcttagact
     4801 tgcccgatgg aatattatct aaattggata tagttggcgc atcaatccat tcttatttta
     4861 atctttcgaa acgtgatcag acagacaggc ttaaaaaagc catgtctaat aaaaatgtcg
     4921 atattatttt tcatccgaca gggcgtctta tcaataagag gccggcttat gagatagata
     4981 tagaagaaat tattgagttt gccgaaaaga cgaaaacggt gttggaaata aattcttttc
     5041 cagatcgcct tgatttaaga gatgagcaaa ttaggatgtg cgttcaaaag ggagttaaga
     5101 tgtctattga ttcggatgct cattccatgg gccatatgaa atatttggaa tatggtattg
     5161 cacaagctag gcgcggctgg gctaagaagg gtgatataat caatgcatgg cccatcgata
     5221 agatgttagc cttcctaaaa gagaataaat caaaatagca tgacgaaaga aatctcggca
     5281 ggcctcatag tttatcgccg gaccaaaggc ggtattaaat ttttgttgct ttatcatggg
     5341 ggtaaatatt ggaattttcc aaaaggtcat atagaagcag aggaaaagag catggagact
     5401 gctatcaggg aaacgactga ggagactggt ttgagaagag tagatttaaa aataaaaaat
     5461 agatttaagg gttatgagaa atttactttt tttagaaata aaaccaagat atttaagata
     5521 gttatatttt atatggctga aaccaacaat cgccagataa gaatttctaa agaacacgat
     5581 ggattcgcct ggttcactta taaagaagct tataatatcc taaagaaata tagagacagt
     5641 attaatgttc taaacaaggc caacgaattt atagttttag gagacaaatc aattaaaccg
     5701 acccccgcat cttaagatgt tgcctcagac agtatttttt taaagactgc cggattgttt
     5761 tcggctaaat ccgctaatat ctttcgatcg attttaatcc ctttcttttt cagggcgttt
     5821 ataaatttac tgtaggttaa gttttcgttt ctgacctccg aatttatttt agacgtccaa
     5881 agagttctgt aatctctttt tttctgtttt cttcctcgga aagcgtttgt ccatgcgtgg
     5941 agaagagctt ctttagcagc tctctcatgg gtttttcttc tccaaccaaa acccttcgta
     6001 tatttaagag ttttagatct tttctttttg tttaatacgc ctcgtttaac tctagtcata
     6061 tttatctatt ttgatatttt ataattcttt tatagtcttg agcgctaatc tttgcagatt
     6121 ttctcttatt tcgcaagtat ttctgtggtt tgcctgcgcg aaaatggtcc tgcgccattg
     6181 tgcgtctcat catcttgcca gttctcgttt ttcttattct ttttgttacg ctttttttca
     6241 ttgttgtttt attggatcaa tttgggtgga gaatactcct ccgaaaaatt taatttcttg
     6301 agttgttttg tggggatggg ttatgagctt cataaattca tttatctttt ctttagccca
     6361 gtctttcatt tgtttctctc ttcctcttaa gcgcatgcga acttctattc tgtgccctcc
     6421 ttcaagaaat ttgtctatcc tttttgattt aatcgacaag tcgttttttg cctcccttgc
     6481 gcttatttgg atttgtttca tttctggaac cttttgagct tgtttttgtt ttttcagctc
     6541 tttagctctc tgataacgaa acttatcata acttatgatt ttagctatcg ggggcgttgc
     6601 gcttggaacg attaatatta gatcaagctc tttagctttt gcttttgcga gggcgtcttg
     6661 taaagataga actcccaggt tgccttcccc ttcatctatt actctaagct ccttagctgt
     6721 tatttgattg ttgattttcg gttcttgttg gatgagaaga ataatagctt aagattttta
     6781 agatatcaac cccgcttgtg ggcatgggga ttttattcga tttcgctgtt ttagggctgt
     6841 tttctatggt aaaaaaatag ccccgattaa ttatcggagc cgttaactta gtttagaagc
     6901 gattcatcgc ttccggcatg caggtttgcc cgtaatcctc ttttagtacg aacgcttcgc
     6961 cgtcatacca cccagcgttc tgtatcggcg gcagagagtc gtgaatctct gctaacgaaa
     7021 acagattgat gttagaaagg cagttccgct tgtagtgtga attgccagtc atgacatctt
     7081 ctggctggtt gtcgaagcat ccataggttg gggagcattg ttcgttcggc gggaaagagc
     7141 cagtgtggcc tgcgccaatg cagtggccga attcgtgcag aacgatattt cgcagatcag
     7201 ttggttggtc gctctcctgc atcgctccta ttccggcatt tgttgcgagg caatattgct
     7261 tcccgctaac atggttgtaa ttcccgcttg cgagtcctgc gtgttgagat ccggcatgcg
     7321 gatcacttac catcgcgatg tcgattatta tatcggcatc cataaactcg gcatatccgg
     7381 catcatatcc caacactttt gtttcccagc taaggtttgc gagatgtggg aattcaggag
     7441 cgttttttcc aataaaccac gcccaatact ctgttgcttc aagcgccgcc tggactgccg
     7501 gatggcgata tagcgcctcc gacccgatcc aatcggggtt taagagcatt gcttcagacg
     7561 agaaatatac tggcggcaag at
//
LOCUS       DATDNZ010000072         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1540091, whole genome shotgun sequence.
ACCESSION   DATDNZ010000072 DATDNZ010000000
VERSION     DATDNZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540091"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..455
                     /locus_tag="VI432_02785"
     CDS             183..455
                     /locus_tag="VI432_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497049.1"
                     /translation="MHFWENIFRIPEYYEMVGVGRKSAGKLKSEIKIEMDEQNTGMPG
                     SGDSDTGTPMPDAPGTGMPETDSPAPADAPAEAPEGAPEQNPPAGM"
     gene            561..>2387
                     /locus_tag="VI432_02790"
     CDS             561..>2387
                     /locus_tag="VI432_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY4497050.1"
                     /translation="MKFLRKNHFYYLLKIGGLSILYYFAAKFGLGFDPVSGFATLIWP
                     PTGIALAAVFLLGYKMWPGIFLGAALINFHTGAPLLVALGIGLGNTLEALAGTYLLKK
                     FKFNKEFEKLNDVWEIIFLAAGASTIISATIGVLSLYLGGVVASSSAPTTWLAWWFGD
                     ALGTLIIAPLIFVWSSNFKTRKTRLYETLAFVSFFLIVNFLVFSETIQINPPFIVSLS
                     ALLLIPLIWAGVRFGNRVAITLIFISSTLAILNTANGYGPFVVSTLTNNLAALQTYIG
                     IVTITILILSTVIKETELNQQLFRNLIEHSYDGILMINVQGKIIYKSSSSSRISGYGQ
                     EELINQDLFTLIHPDDIPKTQEEFKNLLNHPGETAEIEVRIKRKNNKYIWIHATATNL
                     LQEPTVKAIVVNYRDIDEHRRIDKLMGEERVKDEAILKNIGDAMITTDENMKIIMINN
                     EAENLLGWKLEETIGKPIHKIATAYDEKGKELNYEKQPVAISMKTGKRFEATIPTHQY
                     YCAKINGEKFPADFIATPISLKNQIIGMILLIRDASREQKIDLAKTEFVSLASHQLRT
                     PLAIIRWYSEMLSGKNLPKAKEGKYLNEIQAANKRMIELVNAL"
BASE COUNT          824 a          489 c          455 g          619 t
ORIGIN      
        1 attcttcggt tttctaaaat ttctctggca gactctggaa taaaaatata atcgcctccg
       61 gagaatttag ccttcaagct attaaaaaca gttgttttgc cggtagacaa acttccaaca
      121 aaagcgattt ttttagtaaa aacttctccc atggctataa ttataaatct tataatcaac
      181 tattgcattt ctgggaaaat atatttagaa ttcccgaata ctatgaaatg gtcggcgttg
      241 gtcggaagtc ggctggcaaa ttaaaaagcg aaataaaaat agaaatggac gaacagaaca
      301 caggtatgcc tggctctgga gattccgaca ccggaacccc gatgccagat gcaccaggaa
      361 cgggaatgcc cgagacagac tctcctgctc cggcggatgc accagctgaa gctccagaag
      421 gagcgccaga gcaaaacccc ccagcaggaa tgtagaatat aaaacctccg caattgcgga
      481 ggttttatat taaaaaatgc tataattaaa gaattagtca atctcatcta cgttttttct
      541 agcaacaaac aatttaaaaa atgaaattcc tgcgcaagaa tcatttttac tatctattaa
      601 aaatcggagg gctttctatc ctatattatt ttgcggcaaa attcggcctt ggatttgatc
      661 ctgtcagcgg attcgcaacg ctaatctggc cgccaactgg cattgcgctc gcagccgttt
      721 ttcttctcgg atataaaatg tggcctggaa tatttttagg agcggcgtta ataaatttcc
      781 acaccggggc tcccctttta gtcgcccttg gcatcggact gggtaataca cttgaagcct
      841 tagcaggcac atacctcctt aaaaaattta aattcaacaa agagtttgaa aaactaaacg
      901 atgtttggga aattatattc ttggccgcag gcgcaagcac aataataagc gctacgatcg
      961 gagtgttaag cttatatctt ggaggagtgg tcgcatcaag ttctgccccg actacttggc
     1021 ttgcctggtg gtttggcgac gccttgggaa cccttattat cgccccactg atcttcgttt
     1081 ggagtagtaa ttttaaaaca agaaagacac gtttatatga aacacttgct ttcgtgtcgt
     1141 ttttcttaat agttaacttc ctcgtcttca gcgaaacaat acaaataaat cctccattta
     1201 tcgtatctct aagcgcactc ttactcatcc cgcttatctg ggccggagtt agatttggaa
     1261 accgcgttgc tatcacacta atatttataa gttcaacact cgctatatta aacacagcaa
     1321 acggatatgg tccatttgtc gtctcaactc ttaccaataa tctagctgcg cttcaaactt
     1381 atatagggat cgtcaccata acaatattaa tactcagcac agttattaag gaaactgaat
     1441 tgaatcaaca attgttcaga aatctgattg agcatagtta cgacggcatc ctaatgataa
     1501 acgtccaggg gaaaatcata tataaaagct catcaagctc tcgtatctct ggatatggcc
     1561 aggaagaatt aataaatcaa gacttattta ctctaatcca tccggatgac ataccgaaga
     1621 ctcaagaaga gtttaaaaac ctattaaatc atccaggaga aaccgccgaa atagaagtga
     1681 gaatcaaacg taaaaacaat aaatatattt ggatccatgc tactgcaacc aatctattac
     1741 aggaaccgac cgttaaagca attgtagtaa attacagaga catagatgag cacagaagaa
     1801 tagacaagct tatgggagaa gaacgggtga aagacgaggc tatcctaaaa aatatcggag
     1861 acgcgatgat aacaacggat gaaaatatga aaattataat gataaacaac gaagcggaaa
     1921 atttactcgg ttggaaactt gaagaaacaa taggcaagcc aattcataaa atagctactg
     1981 cgtacgatga aaaaggcaaa gaattaaact atgaaaaaca gccggtggcc atatccatga
     2041 aaacaggaaa aagatttgaa gcgacaatac cgactcatca atattattgc gccaaaatta
     2101 acggagaaaa attccctgcg gattttatag cgactccaat ctcgcttaaa aatcaaatta
     2161 taggaatgat acttcttata agagacgcaa gcagagaaca aaaaatagat ctggcaaaaa
     2221 cggaattcgt atcgctcgct tctcaccaac taagaacccc tcttgccatc atcagatggt
     2281 attcagaaat gctttccgga aagaatctgc cgaaggctaa agaaggcaaa tatttaaatg
     2341 agatacaagc cgctaacaaa agaatgatag aacttgttaa cgcgctc
//
LOCUS       DATDNZ010000073         5378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2073543, whole genome shotgun sequence.
ACCESSION   DATDNZ010000073 DATDNZ010000000
VERSION     DATDNZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073543"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..340
                     /locus_tag="VI432_02795"
     CDS             119..340
                     /locus_tag="VI432_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497051.1"
                     /translation="MLNKKIFKNLIYPTTLIVFISISVFIFIKTSSFLYTSINRSFNI
                     DQLRVQSELLNLDLSSFNSLKNRLGIEEN"
     gene            complement(337..657)
                     /locus_tag="VI432_02800"
     CDS             complement(337..657)
                     /locus_tag="VI432_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497052.1"
                     /translation="MDRKAIDFFEEIINDAEPIVDLSEQPDKNWIVKVKDYVLSLIGG
                     LNISRDKAKKSLRSEDEGNNDERAESVDSKVASESSMDLNKSDAGKESREEENIDNKE
                     EGGN"
     gene            784..857
                     /locus_tag="VI432_02805"
     tRNA            784..857
                     /locus_tag="VI432_02805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gly,seq:ccc)
     gene            complement(918..1196)
                     /locus_tag="VI432_02810"
     CDS             complement(918..1196)
                     /locus_tag="VI432_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY4497053.1"
                     /translation="MLTRIIIYAVFAIIIVNLGYQIYKLDINRRFLKAEFSEISGKLS
                     ELESENGDIEEQIEYFSHEANLEKEARARFNYKKPGEKLIIVVPEESE"
     gene            complement(1240..3063)
                     /gene="lepA"
                     /locus_tag="VI432_02815"
     CDS             complement(1240..3063)
                     /gene="lepA"
                     /locus_tag="VI432_02815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY4497054.1"
                     /translation="MKNIRNFVIIAHIDHGKSTLADRFLEVTGTVPAHKMQAQYLDSN
                     ELERERGITIKMAPVRMRYAPRIPASSPRASAQVEEYILNLIDTPGHSDFSYEVSRAL
                     KAVEGAILLVDATQGIQAQTLANFNSAKRAGLKIIGAVNKIDLHPAGSDAVISDLAKL
                     LEVDEADIYKVSGKTGEGVESLLQAVVDLIPPPKEANSRSALVFSSLYDNHKGVVAFV
                     RVFGGVFKKEEQVMLAAGEYKFKIKEVGYFLPVLNSSDRISQGEIGYIATGIKDPNVI
                     KIGDTIMPLSGGKALPGFQIPQPAVFVSIYPNESDDYDDLKAAIAKLQLKDSSLVFSP
                     DFSQVLGRGFKCGFLGRLHFEITIQRLEREFNLSVVNSFPSVAYKIKTKEMNVPVGED
                     GYITIENPKDFPDDYIEVLEPITSVEILTPSRYLGSVMELRSIFKMDNLQTITRGADN
                     IEIKADLPLSELILDFDDKLKSVSSGYASFSYEMKGFLPARVKRLDIVVAGEVVHGLS
                     RVVYEDSLDSEGRKMTTKLKDILPKQQFAQAIQAVVGGRIIARETIPALQKRLGDFGK
                     TGGDRTRKMKLWKKQKEGKARLKETGRVNISTEVFKELLKK"
     gene            complement(3072..3851)
                     /locus_tag="VI432_02820"
     CDS             complement(3072..3851)
                     /locus_tag="VI432_02820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4497055.1"
                     /translation="MDASKVFTKDYYDTVSDLTSLETTLKQVDFLKKALHLKRGARIL
                     DIPCGNGRHSIQLAKAGFRVTGSDSSSPLLKIAKEEAEAMGVYLELKKGDMRRVKIGK
                     RFDAVINMFTSFGYFDDEGTELFLQNIKRHLKPGGKFLIDCENPARLMRSVFEKGKYN
                     KKKNVFIHKILRRPRPGEIRKLERNFDPIRMNWDVKVYWSSVDDKETIQRYKFRLYRL
                     PELRSILKSNGMRIRNVWGDFDGSPLNNKSARMIVLAENLE"
     gene            complement(3858..>5378)
                     /locus_tag="VI432_02825"
     CDS             complement(3858..>5378)
                     /locus_tag="VI432_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEY4497056.1"
                     /translation="GRLLLSLKKVDERLVDLKEKGSYLRNLVYKFDALTPDMNSEYLN
                     LSMDLDYAISGLRAIIGFLDDQSPKHIVVIFENPSEIRPGGGFFGSYADLIIQNGAIK
                     EINVDDIYRPDKFSDFKVVPPLHLQGVTTLWGARDANWFFDFRKSASKIMLFLEESDI
                     FKDNGIQFDGVIAINVRLVEDILKIIGDIDLPEYDLVLNSDNFLREVQYEVEAGRDKI
                     PGQNPKKILSVITPRLIEALKNLEDEDKIKFLDDLNYRLSNKDIKFFFEDENLQNFVA
                     KAGADGAVFETSKNFNGDYLAVVSANVAGGKSDYFIKQIINLNSRIDSSGMINNSLSI
                     TRTHSGEKEKEWWYRAQNNNFIKIFTTAGSKLIDVDGNSVKTITPSVDYKKSGYLVDK
                     DLEEVEGSKELLAHLNTGVYRESGKTVFGTWFNVPAGKSKNLQLIYESSRKISLSNKV
                     NYQFVLDKQSGVDSAFEYAIEAPAGFKWLESDSNLFKYSADNTESRLIINLTLIQL"
BASE COUNT         1609 a         1261 c          775 g         1733 t
ORIGIN      
        1 ttaacttcgt atccggcata acatccatat cactatcaaa tgatgcaaca tggaataacc
       61 tgtcttcaat atcaatgaaa ggaattttat tagtaagatc accggagata acagaataat
      121 gctaaataaa aaaatattta aaaatttaat atatccaaca acgcttatcg tctttatctc
      181 aataagcgtt tttatattta taaaaacatc atcattttta tatacatcaa taaaccgatc
      241 tttcaatatt gatcaattaa gggtgcagtc tgaactgttg aatttagact tatcaagttt
      301 taactctcta aaaaaccgtc ttggaataga agaaaattaa ttacctcctt cctctttatt
      361 atctatattt tcttcttctc tactctcttt acccgcatcc gatttattca aatccatgga
      421 agattctgac gcaactttac tatcaacgct ttcagctctt tcgtcattat taccctcatc
      481 ttcactacgc agagactttt tagctttatc tctagaaata ttaagtcctc ctatcaatga
      541 caaaacataa tccttaacct taactatcca gtttttatcc ggttgctcgc tcaaatccac
      601 aatcggctcg gcatcattaa taatttcttc aaaaaaatct atagcttttc tatccatagt
      661 aatcaatatt aagtataaaa tatttagtat ataaattgaa attatcggct tattatataa
      721 tgtaatagcg tgagtgtggt atattggcaa catacggcct tactccatcc tatacagcag
      781 tatgcgggtg tagcacagtg gcagtgcgct tgcttcccaa gcaagatacg cgggttcgat
      841 tcccgtcacc cgctccaagc acaacaaaac cgctgatcta tcctaccctc cagctaacga
      901 gcaatcttgc caacgattta ctcactctct tctggaacta ctataatcaa cttctctcca
      961 ggtttcttat aattaaatct cgctctggcc tctttttcta aattcgcctc gtgcgaaaaa
     1021 tattcaattt gttcttctat gtctccattt tcactctcca gctctgataa ctttccgcta
     1081 atttcactga attcagcctt taaaaaccgt ctatttatat caagtttata aatctgatat
     1141 cccaaattga ctatgattat cgcgaatact gcataaatta taattcttgt aagcacaatc
     1201 tcattctata taatccaccc gctttttcaa taatatatat tatttcttaa gcaactcctt
     1261 aaaaacctct gttgaaatat ttacccgacc cgtttcttta agccgcgctt ttccttcttt
     1321 ctgttttttc caaagtttca tttttctggt tctatcaccg ccggttttcc caaagtcgcc
     1381 taaacgtttc tgtagcgcag gtatagtctc acgcgctatt atacgtccgc caacaaccgc
     1441 ctggattgct tgagcaaact gctgtttagg caaaatatct ttaagttttg ttgtcatttt
     1501 cctgccctct gaatcaagac tatcttcata aacaacgcgg ctaagtccat ggacaacttc
     1561 accggcaaca acgatatcta accttttcac tcttgcgggc aaaaaacctt tcatttcata
     1621 actaaaggat gcatatccgg aagaaacaga ttttaattta tcgtcaaaat ctaaaatcaa
     1681 ctcagataac ggtaaatcag ctttaatttc tatgttgtct gctccgcgcg ttattgtttg
     1741 caaattatcc attttaaaaa tacttcttaa ctccattacg gagccgagat atctagaggg
     1801 ggtcaatatc tctacactgg taatcggttc taaaacttca atataatcat ctgggaagtc
     1861 tttcggattt tcaattgtta tgtatccgtc ttcaccgacc ggaacattca tttcctttgt
     1921 tttaatttta taagcaaccg atgggaaact gtttacaaca cttagattaa attccctttc
     1981 taatctttga atagtaattt caaaatgcaa cctgcccaag aatccgcatt taaaacccct
     2041 gccaagcacc tgactaaaat ccggtgaaaa gacgagagaa gaatctttaa gctgcaactt
     2101 agcaatcgcc gcttttaaat cgtcataatc atccgattcg ttaggataaa tagaaacgaa
     2161 aacggcaggc tgaggaatct gaaatccagg caacgccttc ccgccagaca agggcataat
     2221 agtatcgcct attttaataa cattaggatc ttttatccct gtagctatgt atccaatctc
     2281 tccttgggat attctatctg acgaatttaa gacaggtaaa aaatatccaa cttctttgat
     2341 tttgaattta tactctccag cggcaagcat aacttgttct tcttttttaa aaacgccccc
     2401 gaaaacacgg acaaaagcaa ccacaccttt atggttgtcg tataaggaac taaataccaa
     2461 agccgatctg gaattagcct ctttaggcgg agggattaga tctactactg cctgcaataa
     2521 actctccacg ccttcgccgg ttttgccaga aactttataa atatccgcct cgtccacttc
     2581 tagcaatttt gctaaatcgc taattacagc gtccgatcca gccggatgca gatctatctt
     2641 attaactgct cctattatct tcaaaccggc tcgcttagcc gaattaaaat tagccagagt
     2701 ctgcgcttgt atcccctgcg ttgcatcgac taaaagtatt gcgccctcaa ccgccttcaa
     2761 cgcgcgagaa acctcataag aaaagtcgct gtgtcctgga gtatctatca agttaagaat
     2821 atactcctca acctgcgcag aagctcgcgg actggacgcg ggaatccgcg gtgcatatct
     2881 catacgcaca ggcgccatct taatagtaat tcccctctct ctctccaatt cattcgaatc
     2941 aagatattgc gcctgcattt tatgcgcggg cacggttccg gttacctcta aaaagcgatc
     3001 ggccaaagtc gacttaccat gatcaatatg cgcaattatt acgaaatttc taatattttt
     3061 cacttaatta tttattctag attttccgct agaactatca tcctggcgct tttattatta
     3121 agaggactac cgtcaaaatc tccccataca tttcttattc tcatgccatt cgattttaga
     3181 atgcttctca actccgggag tctgtaaaga cggaatttat atctctgaat cgtttccttg
     3241 tcgtccaccg aactccaata taccttaaca tcccaattca tcctaatggg atcaaaattc
     3301 ctttctagct ttctgatctc tccgggtcgc ggtctgcgca aaattttatg aataaaaaca
     3361 tttttcttct tattatattt tcccttttca aacacgcttc tcatcaagcg cgcgggattc
     3421 tcgcaatcta ttaaaaactt tcctcctggc ttaaggtgcc tttttatatt ttgaagaaat
     3481 agctcagtac cctcgtcgtc aaaataacca aatgaagtaa acatattaat aacagcgtca
     3541 aaacgtttgc ctattttaac gcgcctcata tccccctttt ttaactcaag ataaacgccc
     3601 atagcttccg cttcttcttt agcaattttc aaaagagggc tcgatgaatc agagccagtc
     3661 accctaaacc cagctttcgc gagctgaata gaatgccttc cgtttccgca cggtatgtca
     3721 agaattctgg cgccgcgttt aagatgtaat gcctttttca aaaaatctac ttgctttaac
     3781 gttgtctcaa gactggttaa atcgctaacc gtgtcataat aatcttttgt aaaaacctta
     3841 gaagcatcca taggaagcta aagctggata agcgttaaat ttataatcaa tctactctcc
     3901 gtattatctg ctgaatattt aaataaatta ctatcgctct ctaaccattt aaatcctgcc
     3961 ggagcctcaa tagcatactc gaaggccgaa tctacaccag actgcttatc taaaacaaat
     4021 tgatagttaa ccttatttga taaagatatc tttcttgaag actcatatat taattgtaaa
     4081 ttcttggatt ttccagccgg aacattaaac cacgtgccaa aaaccgtttt acccgattca
     4141 cgataaactc cggtattcaa atgagctaaa agctctttac tgccctcaac ctcctctaga
     4201 tctttatcca ctaaatatcc tgatttttta taatcaacag atggggttat cgtctttact
     4261 gaattaccat caacatcaat caacttagat ccggccgttg tgaatatttt tataaaatta
     4321 ttattttgag ccctatacca ccattccttt tccttttcac cgctatgagt ccttgtaatt
     4381 gaaaggctat tattaatcat tccagaagaa tctattctgg aattcaaatt gataatctgt
     4441 tttataaaat aatctgactt ccctcctgca acatttgcac tcacaacagc taggtaatct
     4501 ccgttaaaat tttttgacgt ttcaaaaacc gcgccatccg cccccgcctt ggcgacaaaa
     4561 ttttgcagat tttcgtcttc aaaaaagaat ttaatatcct tatttgataa ccgataattc
     4621 agatcgtcta aaaacttgat cttgtcctcg tcttctaaat ttttaagcgc ctctataaga
     4681 cgcggtgtta taacgcttaa aatcttttta ggattctgac ccggaatctt atcccttccc
     4741 gcctcaacct catattggac ttctctcaaa aaattatcgc tatttaaaac caaatcatac
     4801 tcgggtaagt ctatatctcc gataattttc agaatatcct caaccaacct aacgtttata
     4861 gctataacgc catcgaactg gataccgttg tctttaaaga tatccgattc ttcaagaaag
     4921 agcataattt tggaagccga ctttctaaaa tcgaaaaacc aatttgcatc acgcgctccc
     4981 cataaagtcg tcacgccctg aagatgcaac ggcggaacaa ctttaaaatc ggaaaactta
     5041 tctggacgat aaatatcatc aacatttatt tcttttattg ctccgttttg gatgatcaaa
     5101 tcagcgtaac tgccgaaaaa gcctccaccc ggtcttatct ctgacggatt ttcaaaaatc
     5161 accacgatat gttttggcga ttgatcatca aggaaaccta ttattgccct tagtcctgat
     5221 atagcataat ctagatccat gcttaaattc agatattcgg aattcatatc aggcgtaagc
     5281 gcatcaaact tataaacaag atttctaagg taggatcctt tttctttcag atcaacaagc
     5341 ctctcatcta ctttcttcaa agataagagc agtctgcc
//
LOCUS       DATDNZ010000074         4565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2075179, whole genome shotgun sequence.
ACCESSION   DATDNZ010000074 DATDNZ010000000
VERSION     DATDNZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075179"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..914)
                     /locus_tag="VI432_02830"
     CDS             complement(384..914)
                     /locus_tag="VI432_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY4497057.1"
                     /translation="MLTDIKSITPIITSIAVNILLFLAIFITAYIIAQISRKITKKIL
                     LEKLEQKAIATFFSDLLFYILIIVGLTVALSTTGVNVNAIIAGLGLGGFALGFALKDI
                     IGNFISGLMILLTKPIKIGDSIKISGFEGTVESINLRHTTIRSVGKIKEKILIPNNTI
                     FSSITVIQKEKTKTKS"
     gene            936..1622
                     /locus_tag="VI432_02835"
     CDS             936..1622
                     /locus_tag="VI432_02835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY4497058.1"
                     /translation="MRIQKYLSEKGIMSRREAERAILAGKISVNGKVVTNLATQIDGV
                     KDKVRVVDVPGKSMTIALNKPRGIVSGRNRNEGKTIFNILPAKFSFLNAVGRLDKESE
                     GLLLLSDDGIVTRAVTGEDHLIEKEYEVRVREPLKPRYIRKMEEGVQLEDGKTLPAEA
                     EMLGEYAFRIVLKEGRKHQIRRMAAKFGLTILELKRVRIGNIRLGNLRPGEYKDLSGK
                     EVDSLKILAK"
     gene            complement(2073..2396)
                     /locus_tag="VI432_02840"
     CDS             complement(2073..2396)
                     /locus_tag="VI432_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY4497059.1"
                     /translation="MKKDKSFHDYIVHDILEGIPNISSKAMFSGWGIYKNGKIFAIIT
                     DGELYFSARGERGDEVKKYLENLGSHPFAYNRKGKLVTLSYWLAPEELFENREALYEF
                     INKYY"
     gene            2515..3390
                     /locus_tag="VI432_02845"
     CDS             2515..3390
                     /locus_tag="VI432_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY4497060.1"
                     /translation="MAEGKTKIIRPGSRLGTVVIESKDDITAGDGARRAILNRKSVYA
                     TTTTANVFKLLNAAGIRTHYVRRLSSRKFEARHVGMIPLEIVIRRIATGSYLRRNPGV
                     VEGTIFDPLVVEFFLKDDARHDPFIRFAWEEGNMWLFDAKSPSDEAIGIESLDDWRIQ
                     GKRKARLRLIDEHLVIDLGAIARHVFLTLETAWKRLEVDLVDLKIECGFDRETGELLV
                     GDVIDNDSWRIWPLGEKDQAQDKQVFRDLKEVTDEAMDDVGDKYRRVAAWTDRFLEQR
                     PRATSSWRLAQAIAS"
     gene            complement(3461..4249)
                     /locus_tag="VI432_02850"
     CDS             complement(3461..4249)
                     /locus_tag="VI432_02850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4497061.1"
                     /translation="MKNILIISVILIVVLAGFYFFGYKTETKDSNNADMLSGNGSLRI
                     KSEEINYFKNINGFLARPESDGPYPGIVMIHEWWGLNENIKDMAQELAKEGYAVLAVD
                     LFGKVATTSDEARTQVSSLNQDLALSNLRAATAYLRNEGVEKIASLGWCFGGGQSLQL
                     SLSGEELSATVIYYGQLTSDEKQLSMIHWPVLGIFGEEDQSIKVETVNDFENTLNGLG
                     KTNDIYIYPAVGHAFANPSGTNYAPEETKDAWAKTLTFLAANLK"
     gene            complement(4270..>4565)
                     /locus_tag="VI432_02855"
     CDS             complement(4270..>4565)
                     /locus_tag="VI432_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4497062.1"
                     /translation="IKLAIENHPDIILLDIIMPKSNGLNLLHSLRQDSWGKNVPVIIW
                     SNVSDPQTRKHAEEHGIKTYLIKTDWSLNEAVGKIKEELESHATDKKTSLIKL"
BASE COUNT         1174 a          849 c         1069 g         1473 t
ORIGIN      
        1 atgttaattt ttataagtct aatatgtttt ataagaaaaa gtttagcccg gactcaaatg
       61 agttcgggct ttattcgtgt tgtgggggag gaaagacgtg gaggtgaaga ttgagcctgg
      121 ctgatttttt agaccagcca ggctgggggg aggacgccgt gttgatggtg aatttggctt
      181 aacctccttt tcgcgcgttt tttgtcgttc gtgtaggtgg aacttcttgc cgccctactt
      241 gacgctcgtc gtgatgttgt tgcgcacgtt tttcatgacc ttggtcagcg ttattatagc
      301 tgcctccttt gtcctttcgc gtccctgacg taaaggagct accctaaata atatcatatc
      361 tatttttata cggttgtgga taactatgat tttgttttgg ttttttcttt ctggattact
      421 gtaattgagg agaagattgt attgtttgga atcagtattt tttcttttat cttgccgaca
      481 cttcttattg ttgtatgccg cagattaata gattctaccg tcccttcaaa accggatatt
      541 ttgattgagt cgccgatttt aataggtttg gttaaaagaa tcatcagccc gcttataaag
      601 ttgccaataa tatcttttag agcgaaccct aaagcgaatc ctcctaatcc tagtcctgcg
      661 atgatagcgt ttacgttcac tcctgtggtt gatagagcta cagtaaggcc cacaataatc
      721 aatatataga aaagaagatc cgaaaagaaa gtagctatcg ctttttgttc caatttttcc
      781 aatagtattt ttttcgtgat tttcctcgat atttgggcta tgatgtaagc cgttataaat
      841 attgccaaaa ataataaaat atttacggct atagatgtta ttatcggagt aatactcttt
      901 atatctgtaa gcatattatt attttatcac tttagatgcg tattcagaaa tatttatcag
      961 aaaaaggaat aatgtctcgt cgtgaagcag agcgcgctat tttggcgggc aaaattagtg
     1021 ttaatgggaa agttgttacc aatctcgcta ctcaaattga tggagtgaaa gataaagttc
     1081 gggtcgtaga tgttcctggc aagagtatga caatagcttt aaataaaccg cgaggaatag
     1141 tctctggacg taatagaaac gaaggcaaaa ctatttttaa tattctgccg gcaaaatttt
     1201 cttttttgaa tgcggttggg aggttagata aagagagtga gggtttattg ctgttatcgg
     1261 acgacggaat tgttactcga gcagttacgg gagaagatca tttaattgaa aaggaatatg
     1321 aggttagagt cagagagcct ttaaagcctc gttatataag aaagatggaa gaaggggtgc
     1381 aattagaaga tggaaagact ttgcccgcag aagcagagat gcttggagag tatgcgttta
     1441 gaattgtttt aaaagaggga agaaagcatc aaataagaag gatggccgct aagttcggct
     1501 tgactatttt agaattaaaa agggttagga tcggtaatat aagattaggt aatttgaggc
     1561 ctggcgaata caaggatcta agcggtaaag aggttgatag tttgaagatt ttagcgaagt
     1621 gattttttaa aaagattgcc cgcatcatgt tcgtgcgggc ttgtttatgg aggaaggagg
     1681 ggattgagat tgggataaga cttggagaac aagcttcttc gtttgcccta ccttgaggtg
     1741 ggcggagcgg gcatacggcc actggtcggt ttgccgaatg tccctctgtt gggccttcgg
     1801 tcacggatcg ttgaccattt gtcgttccgt ctgaatgttt tgctgatttg cgcacctcct
     1861 tctttgttgg attgagaggg ctgagagtgg atgaatcccg agtgtctagc tcttcttgtg
     1921 cgctagcgtc tcgggtagtt gtgcgggcgg ttctggcctt gtcctttgcc gctcgtgttc
     1981 tttgggggcg gggacgactt ttcctttcct atcaaatcac ctcctatacc gtaaaggtac
     2041 agttgtttag aatatcataa taattatcgg agtcaataat atttattgat aaattcatat
     2101 aatgcttcgc ggttttcaaa gagctcctct ggcgcgagcc aataagatag cgtaaccagt
     2161 ttaccttttc tgttataagc gaatggatga ctgcctaaat tttcgagata ttttttaact
     2221 tcgtctcctc tttctcctcg tgcggaaaaa tataattctc catcggtgat gattgcaaat
     2281 attttgccgt ttttatatat gccccatccg ctaaacattg ccttgcttga tatgtttggg
     2341 atgccctcta gaatgtcgtg gactatataa tcatggaatg atttatcttt tttcatcgcg
     2401 attattataa tgcttaattg attttcgtgc tcgacttgcc ttatcaaata tttttattta
     2461 tgatgtggcc actccaataa aggaggtgag aaaatagctc gtttaaaaac aaaaattgct
     2521 gaaggcaaga cgaagatcat ccggcccggt agtcggttgg gaacggttgt tatcgaaagc
     2581 aaggatgaca ttaccgccgg tgacggcgcg cgaagggcta ttcttaatag aaaatcggtt
     2641 tacgccacca cgactacggc taacgttttc aagcttttga atgccgcggg catccggacg
     2701 cactacgttc gacgcctctc ttctaggaag tttgaggcgc gacatgtggg gatgattccc
     2761 cttgagatag tgatccgtag gatcgccacc gggtcttact tgaggaggaa tcctggcgtc
     2821 gttgagggga cgatttttga tcctctcgtt gttgaatttt tcctcaagga cgatgcgcgt
     2881 cacgaccctt ttatcaggtt tgcctgggaa gaaggcaaca tgtggctttt cgatgccaag
     2941 tctccgtcag acgaggcgat cggcattgag tccttagatg actggagaat ccaaggcaaa
     3001 aggaaggcaa gacttcgcct gatcgatgaa catcttgtta tcgatcttgg ggcgattgcg
     3061 cgtcacgttt tcctgacact tgagaccgca tggaaaaggc ttgaagtcga cttagttgat
     3121 ctgaagatcg aatgcggttt tgatcgcgaa accggcgagc ttcttgtggg ggatgtgatt
     3181 gataatgatt cttggcgcat ctggcccctg ggagagaagg atcaggcgca agacaagcag
     3241 gttttcagag acttaaaaga agtcaccgat gaggcgatgg atgacgttgg cgataagtat
     3301 cgccgagtcg ctgcctggac cgatagattt cttgaacaaa ggccaagagc gacttcttcg
     3361 tggagactcg cccaggctat tgcaagttag tggctgaatt tttagccccg ctttaatcca
     3421 agcggggctt aatttattag ttttattttt ttcggctcga ctatttcagg ttagcagcaa
     3481 gaaaggtaag tgtttttgcc caagcgtctt tagtctcttc tggggcataa ttagttcctg
     3541 atggattggc aaaagcatgc cctacagcag gataaatgta tatatcatta gtttttccca
     3601 atccgtttaa tgtattttca aaatcgttta ctgtttcaac cttgattgat tgatcttcct
     3661 cgccgaatat tcccaaaaca ggccagtgga tcatggagag ttgtttctcg tccgaggtta
     3721 attgtccgta ataaataact gttgccgata attcttcgcc ggataaagag agttgtaaag
     3781 actgtccgcc gccaaagcac caacccaatg atgcgatttt ttctacaccc tcatttctta
     3841 agtacgctgt cgcggctcgc aggttggaga gggccaaatc ttgatttaga gagctgactt
     3901 gtgttcttgc ttcatcggat gttgtcgcaa cctttccaaa taaatctact gctaagaccg
     3961 cataaccctc tttggctaat tcttgagcca tatctttaat attttcattc aatccccacc
     4021 attcgtggat cataactatg cccggatacg gaccgtctga ttcaggcctg gctagaaatc
     4081 cgtttatatt cttgaaataa tttatttctt cgcttttgat tcttaggctt ccattgccgg
     4141 agagcatatc ggcattattt gaatctttcg tttcggtttt atatccaaag aagtagaaac
     4201 cggctaatac gactattaat atgactgaga ttataagtat atttttcata tcaaattaaa
     4261 ttatatcaat cagagcttta ttaagctagt ttttttgtct gtggcatgag attccaactc
     4321 ttcttttatt tttcccactg cttcgttaag cgaccagtcg gtttttataa gatatgtttt
     4381 tattccgtgt tcctcggcgt gtttcctagt ttggggatcg cttacattag accatattat
     4441 aaccggcaca tttttccccc aagaatcttg gcggagactg tgtaacaagt ttagaccgtt
     4501 tgatttaggc attattatat caagcaatat tatgtcggga tggttctcga ttgcgagttt
     4561 aattc
//
LOCUS       DATDNZ010000075         5013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_480676, whole genome shotgun sequence.
ACCESSION   DATDNZ010000075 DATDNZ010000000
VERSION     DATDNZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480676"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VI432_02860"
     CDS             <1..1764
                     /locus_tag="VI432_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497063.1"
                     /translation="SLYINGELKGSASAIGANTTSLAPFTIGAWDDRFGVTEIFAGQI
                     KEVTIYKGSRKNIKFPIKELGNCGSEVECRAYCDDDKNVLTCIEYSINNDLLSGEEKE
                     EAKKVLEFLKKGENLPGGCATREECVSYCSEESNGDECVEFTLKAGLVQPDEVEEYKK
                     VRQYIASGETPGGCKTKNACDRYCSDTANIEECFAFAEKAGLIPSGEIEQARKAIEFM
                     KRGEMPGGCRNKEECMTYCEVESHMAECIEFGVKAGFMTEEEAKLAKRFLESGGPGGC
                     KGREECDHFCNDPKNQSACFDFAVEAGFIAEEDAEFFKKQMEQGGGESRQTGGFEQMP
                     AKVRECLDNRLGSGIAAKFASGEIAPTREMMSSMRICIEEFQNSCPAFAGMMDDNRGE
                     GDMGQGAEQFNECLIDIYGSDVPEKMKSGDFCPPADFQQKISECMQTKFGNMGGSSGD
                     GGSGGGFQGGGDPGFQGGSQSNYQGGGDFSGPGGCKTEAECRAYCESNPQACMGFGGP
                     NSSGGQSNYDHGADECSNQGGYWQNNQCVFPQQQQEQNQQQNQQQYQGPDCSSFEQVP
                     QCSYITDPQGYEYCKRCYPDK"
     gene            1825..2679
                     /locus_tag="VI432_02865"
     CDS             1825..2679
                     /locus_tag="VI432_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4497064.1"
                     /translation="MLPIVLALIVLVGWGTADIFVTMNSRRLGGYSASFWIYVFGFLF
                     YLLIAWFWIGDLAGLTPQILLINLGTAFVGVIGYMLFVEALRVGNAAVVGTISASYIA
                     ITVFISILFLGETINLYQTLAIILILLGIIISAEKFSNLKQGVFNKGSLLALGTMLAW
                     GIYFAYVKIPAEAIGPIWSNFINSSPFILIFIYMWFRGIKLKMPDKKAIGHLAAGSIL
                     MNIAYVFYNAAIVIGSVAVVSPIAGSAAVLFAVLAHYVFKDEISRQQIYGVGVTLAGV
                     VALSALSL"
     gene            2877..3359
                     /locus_tag="VI432_02870"
     CDS             2877..3359
                     /locus_tag="VI432_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497065.1"
                     /translation="MRNTKAAFLWIVGLLEDLRIIFEVDGGLAAELYGSGRELADIDI
                     NIYKKDFEKLIPKIKEYIKFGPERYKDDHWDIFMITIKYAGQNIDIGAVDDARYFDEE
                     EKKWVSLPADLSDVRVMEYAGINVPVINEIKLMIYKSKLGRGVDKRDVGAMSDKLFNQ
                     "
     gene            3434..3985
                     /locus_tag="VI432_02875"
     CDS             3434..3985
                     /locus_tag="VI432_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY4497066.1"
                     /translation="MSYNFWLEILKDVLVLLWKIWPIWTILLIVVAFRIFGDSILPKL
                     LRLRKSAKNKKWLSGKDYINKIRKYSPKEFEDYIAWMFREMGFKTESVGGSYDGGIDV
                     IAEKDGIKHYIQCKKFIIQEVGVGAMRDFYGAIAGKVTNGKGYFITTNKFTLEAERFA
                     EDKPIELVDQYKLIKWREEIAKK"
     gene            3996..4415
                     /locus_tag="VI432_02880"
     CDS             3996..4415
                     /locus_tag="VI432_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY4497067.1"
                     /translation="MEVVNRSENFYTLSFQPGEDLISGLTEFARENKISAGHITGLGA
                     CGKLHLAYYDLDKKEYIQKVFEERLEILSLVGNISLDPEGKESIHLHGVFGKSDFSTL
                     GGHVMELIISGVGEIHIQILLGAFRRSHDPETGLNFL"
     gene            4481..4750
                     /locus_tag="VI432_02885"
     CDS             4481..4750
                     /locus_tag="VI432_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4497068.1"
                     /translation="MKTAKQLERYFKGAANHRRISILLAVDKRNGMTVEDIAYLLESN
                     FKTISQHTKSLVIAGLLNKKYNGRSVEHSLSPYGRSFIKFIKSFN"
     gene            4797..>5013
                     /locus_tag="VI432_02890"
     CDS             4797..>5013
                     /locus_tag="VI432_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY4497069.1"
                     /translation="MHKNLKIYGNVQGVFFRQSAKIKADELGIKGFVKNEPDGSVYIE
                     ADGDGEAVERFIDWCRLGPDSANVDKIE"
BASE COUNT         1491 a          747 c         1268 g         1507 t
ORIGIN      
        1 agtttatata taaacggaga gttaaagggt tccgcctctg cgattggcgc aaatacgaca
       61 agtctggccc cgtttacgat aggcgcttgg gatgataggt ttggcgttac cgaaattttt
      121 gccggacaga ttaaagaagt aaccatatat aaaggatcaa gaaaaaatat taaatttcct
      181 ataaaagaac ttggcaattg tggaagtgaa gttgagtgtc gtgcgtattg cgatgatgat
      241 aaaaatgttc ttacctgtat agaatattct attaataatg atttactgtc tggtgaagaa
      301 aaagaagagg ccaagaaggt attagaattt ttaaagaaag gagagaatct tccaggcgga
      361 tgtgccacta gagaagaatg cgtgtcttat tgttccgaag agtctaacgg cgacgagtgc
      421 gttgagttca ctctgaaagc gggccttgtt cagccggatg aggtagagga gtataagaaa
      481 gtaaggcaat atatcgcaag cggggagact cccggtggat gtaagactaa aaatgcttgc
      541 gataggtact gttcggatac ggcaaatatt gaagaatgtt ttgcttttgc cgagaaggct
      601 ggattaattc catctggcga gatagagcaa gcgagaaagg ctattgaatt tatgaaacgc
      661 ggcgaaatgc ctggcggatg tcgtaataaa gaagaatgca tgacttattg tgaagtagaa
      721 agtcatatgg cggagtgcat tgagttcggc gttaaggctg ggtttatgac tgaagaagag
      781 gcgaaacttg ccaagaggtt tttggaatcc ggtggtccag ggggatgtaa ggggcgcgag
      841 gaatgcgatc acttctgtaa tgatcctaaa aatcagtctg catgttttga tttcgcggta
      901 gaggccggtt ttatagctga agaagatgcc gaatttttta aaaaacaaat ggagcaggga
      961 ggaggagaga gtcggcagac tggtggtttt gagcagatgc ccgcgaaagt aagggagtgt
     1021 ttggataata ggcttggctc tggaattgct gctaaatttg caagtggtga gattgctcca
     1081 acgcgggaga tgatgagttc aatgaggatt tgtattgaag agttccaaaa ttcttgcccg
     1141 gcttttgcgg ggatgatgga tgataatcgg ggtgaggggg atatgggtca gggcgcggaa
     1201 cagtttaatg aatgtttgat agatatttat ggcagtgatg ttcctgagaa aatgaagtct
     1261 ggagattttt gtccgcctgc cgattttcag caaaagatca gtgaatgcat gcaaactaaa
     1321 ttcggtaata tgggtggatc ttctggtgat ggcggttcgg gcgggggatt tcagggtgga
     1381 ggagatccag gatttcaagg aggcagtcag agtaactatc agggcggagg tgacttcagt
     1441 gggccaggcg gatgtaagac cgaggctgaa tgtcgcgcat actgtgaatc aaatccacag
     1501 gcttgtatgg gatttggcgg accgaattct agtggaggtc aatcaaatta cgatcacgga
     1561 gccgatgaat gcagcaatca gggtgggtat tggcaaaata atcagtgcgt atttccacaa
     1621 cagcaacagg agcaaaatca gcagcaaaat cagcaacaat accaaggacc tgattgttct
     1681 tcgtttgagc aggtgcctca atgttcatat ataacagatc cccaaggata tgagtattgc
     1741 aagcggtgtt atcctgataa ataataattt ctaaaaccgc tgtggcgata tcgtttttga
     1801 tcgattttat tgtatcctga tattatgctc ccaattgttt tagctttaat tgttttggtc
     1861 ggctggggta cagccgatat tttcgttacc atgaattcgc gtaggctcgg tggatattca
     1921 gcaagttttt ggatttatgt attcggattt ctattttatt tattgatcgc ttggttttgg
     1981 ataggagatt tggcgggact tactccacaa atacttttaa tcaatttagg aacagcgttt
     2041 gtgggtgtta ttggttatat gctttttgta gaagcattaa gagttggaaa tgcggctgtc
     2101 gttggcacta tttcagcttc atatatcgcg atcaccgttt ttatctcgat tttattttta
     2161 ggcgagacga tcaatttata tcagactctc gcgataatat tgattttgct cggtattata
     2221 atttcagcag aaaaattttc taatttaaaa caaggagttt ttaataaagg aagcttgctc
     2281 gcattgggca cgatgcttgc ttggggaatt tattttgctt atgtaaaaat tcccgcagag
     2341 gcgattgggc ctatctggtc taattttata aattcatcgc catttatatt gatattcata
     2401 tatatgtggt ttcgggggat aaaattaaag atgccggata aaaaagcgat tggccatctc
     2461 gcggctggca gcattttgat gaatattgct tatgtttttt ataacgctgc tattgttatt
     2521 gggagtgtgg cggtcgtctc tccaatcgcc ggctctgcgg cagtgctttt cgcagttcta
     2581 gcgcattatg ttttcaagga tgagatttct cgccagcaaa tttatggagt tggcgttact
     2641 cttgccggcg tagtcgctct gtctgctttg agtctttagg tcaatgacga gatcagcgag
     2701 ataagagatt tattagaccg ttcagcatct atttttaaac attctaacat tcttaaaaat
     2761 gttagaatgt tgtttagtca tcctcggctt atataaatta tataaaccta gtaaaccgaa
     2821 gggtttggga gtgaaaaagg tggtagaata ggtcagtgaa tactataaat taatctatgc
     2881 gcaacactaa ggcggctttt ttgtggattg tagggcttct tgaagaccta aggattattt
     2941 ttgaagttga cggcgggctg gctgccgagc tttacggctc cggccgggag ttagccgaca
     3001 ttgatatcaa tatctacaag aaagattttg aaaaacttat tcccaagatt aaggaatata
     3061 ttaagtttgg cccagagcgt tataaagacg accattggga tatttttatg ataaccataa
     3121 aatacgccgg ccaaaacata gacatcggcg ccgtggacga cgcgaggtat tttgatgagg
     3181 aggagaaaaa gtgggtctct ttgcctgccg acttatctga cgtcagagtc atggaatacg
     3241 ccggaatcaa cgtccccgtt ataaacgaaa tcaaattaat gatttacaaa agtaaactag
     3301 gcaggggagt ggacaaacgc gatgtcggcg caatgtctga taagcttttc aaccaataaa
     3361 tacaccgact cggcttatat tatttatata agccgaagat ttttggatcc gtgttcggtg
     3421 gtataataaa tttatgtcat acaatttttg gttggagata ttgaaagatg tattggtgtt
     3481 actatggaag atttggccga tttggacaat tttattgatt gttgtcgcct tcagaatttt
     3541 tggagatagt attttaccca aattattgag attaaggaag tctgcgaaaa ataagaaatg
     3601 gctttcaggt aaggattata taaataagat tcgcaagtat tcacctaagg aatttgaaga
     3661 ttatattgct tggatgttta gagaaatggg atttaaaact gaatcagttg gaggttctta
     3721 tgatggtgga attgatgtta tagcagaaaa agatgggatc aaacattata ttcaatgcaa
     3781 gaagtttatt attcaagagg tcggagtcgg ggccatgaga gatttttatg gcgcgattgc
     3841 tggcaaggtt acgaatggaa agggttattt tattactact aataaattta cgttagaggc
     3901 agaaaggttt gcagaagata aacctatcga actcgttgat caatataagt taattaagtg
     3961 gcgagaggag atagcaaaaa aataggattt ttgaaatgga agttgttaat agaagtgaaa
     4021 atttttatac attgagtttt cagcccggcg aggatctgat ttctggatta actgaatttg
     4081 cgcgagaaaa taaaatcagc gccgggcaca ttactgggct tggcgcgtgc ggtaaacttc
     4141 acctagcata ttatgattta gataaaaaag aatatataca aaaggttttt gaagagagat
     4201 tagaaatttt atctctcgtc gggaatattt ctcttgatcc agaagggaaa gagtctattc
     4261 atctccacgg cgtttttgga aaatccgatt tttcaacttt agggggtcac gtgatggagt
     4321 taatcatttc cggcgtaggg gaaattcaca tccaaattct tttgggggcg tttcgccgtt
     4381 cccacgatcc cgaaacagga ttaaacttcc tctaatcaca acattccaac attctgcaga
     4441 atgttggaat gttgtgtggg agatgctata attgttttgt atgaaaacag caaagcaact
     4501 tgaaagatat tttaagggcg ccgcaaatca tcgtagaata tctattttat tagctgtaga
     4561 caaaagaaat ggaatgaccg tcgaagatat cgcttattta ttggagtcaa attttaaaac
     4621 tatctcgcag cacacaaaat ctttggtgat cgctggattg ttgaataaaa aatacaatgg
     4681 tagatcggtc gaacattcat tatctcccta tggccgatca tttattaaat ttataaaatc
     4741 ttttaattaa tatattctaa cattctgcag aatgttagaa tgttgtagaa aggaagatgc
     4801 ataagaattt gaaaatatat gggaatgtgc agggggtttt ctttaggcag tctgcgaaaa
     4861 taaaagcaga tgaattggga attaaaggtt ttgtgaagaa tgagccggat ggaagcgtgt
     4921 atatagaagc tgatggagat ggcgaggcgg ttgaaagatt tattgattgg tgcagattgg
     4981 ggccggattc ggcgaatgtg gataaaattg agg
//
LOCUS       DATDNZ010000076         3747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_486541, whole genome shotgun sequence.
ACCESSION   DATDNZ010000076 DATDNZ010000000
VERSION     DATDNZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486541"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VI432_02895"
     CDS             <1..412
                     /locus_tag="VI432_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4497070.1"
                     /translation="RRIKGSEITEEIEYEMQDMNFINELKAYEAKPKFILCDRSVIDP
                     VVYALAEGDTDRADRLLARMRHWLQTYHQFILFDKEGIPYKQDEFRDEDYKYRDKVHD
                     SFIDFFSSLNIPYVVAAGDSENRTDFIERLLAEK"
     gene            complement(485..1720)
                     /locus_tag="VI432_02900"
     CDS             complement(485..1720)
                     /locus_tag="VI432_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY4497071.1"
                     /translation="MQKIFIVFFGLLIIVASSILWLSNNTGLGKGVDAGAITATIGSP
                     WIGSLFTDETEKMTNRKIAAGTVFALVAEEIGVSPEETLSILEASKEKYNLAKIVEGR
                     QISFVSDRETGVFNKVIYQINADEILAVQKTTDNTWTAERTAIPYEIEAEEIEGTIES
                     SLFETIINSGGDERIALALSEVFAWQIDFAVDIRVEDSFKLVYEKRMLNGNYMGPGNI
                     LAAKFINDGAEYKGIYFASENSKAGYYDMEGNSLQKIFLKSPLSYKYISSGFSYNRLN
                     PVTRSFHAHRAIDYAAPTGTPIISVGDGTVTLAGWNGAYGNTIKVRHNETYTTLYAHL
                     SKFAVNSGSKVKQGQIIGYVGSTGQSTGPHLHFEMYKNGSHTNPLTIELPPGEPIDKT
                     EKALFENKIQQYQNLFSST"
     gene            complement(1761..2957)
                     /locus_tag="VI432_02905"
     CDS             complement(1761..2957)
                     /locus_tag="VI432_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY4497072.1"
                     /translation="MPQFFSFFIILLTAVLFSSLIRRIRVPWVVALIIGGFIIGPHGL
                     KFFEANATIDFLAAIGLVFLMFMAGLETKFSDTKGIKKKIGVVALLSGGLPALVGVAI
                     GLGLGYAWPTAILLGIIFMSSSIALLIPSFQSAGIISSQFGKMIIGATILIDIASLIL
                     LSLFLQSIAGDYNFLIILLYPLLILFLGALAWILPKIRQAFGEYAEEEDLFERELRFV
                     LLILIGLVVLFEFLGLHAIIAGFFAGFILSGSVQTKIARAKLHAVSYGLFIPLFFVVI
                     GSNINLSVLQDATLIFPIAIAVVIGSIGSKFISGWLSGKILGFTAGESAFLGASTIPS
                     LSTTLAVAFLGFNQGILTQELLTSVVLLSIVTSIVSPFLVGLFSKRIESQKTLLRGLA
                     EKEIQN"
     gene            complement(3000..3296)
                     /locus_tag="VI432_02910"
     CDS             complement(3000..3296)
                     /locus_tag="VI432_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497073.1"
                     /translation="MKLILGIIVMVILAVGIGYYFGYDIGWEKAIDENGTELTECRPT
                     GCSNHICSDEDVITTCEYLPEYACYQNARCERQKNGECGWTPTEELNACIAEYK"
BASE COUNT         1051 a          768 c          763 g         1165 t
ORIGIN      
        1 ccgaagaata aaagggagtg agattacaga ggaaatagaa tatgaaatgc aagatatgaa
       61 ttttatcaat gaacttaagg cttacgaagc caagccgaaa tttattttat gcgacagatc
      121 tgtgattgac cctgttgttt atgcattggc tgaaggagat acagatagag ccgataggct
      181 tttggcgaga atgaggcatt ggttacaaac atatcatcaa ttcatcttgt tcgataaaga
      241 gggcattcct tataagcagg acgaattccg ggatgaggat tataaatata gagataaggt
      301 ccatgattca tttatcgact ttttctcttc tttgaatatt ccttatgttg ttgcggctgg
      361 ggattctgaa aatagaacag attttataga aagattgctt gcggaaaaat gataaaaacc
      421 ccgtgattac atagatttta tgtaattaat ccaattataa agatttagtt tctatgcaga
      481 ctttctatgt agaagaaaat aaattctgat attgttgaat tttattctca aaaagcgcct
      541 tttctgtttt gtctatcggc tctccgggcg ggagctctat ggttagtgga tttgtgtgtg
      601 agccgttttt atacatttcg aagtggagat gcggtccggt tgattgcccg gtcgagccca
      661 catagcctat gatttgcccc tgtttaactt tagatcctga attgacggca aattttgata
      721 gatgggcgta gagagttgta tatgtttcat tgtgtcggac cttaatcgtg tttccatatg
      781 ctccattcca gcctgcgaga gtcacggttc cgtctccgac agatatgatt ggagttcctg
      841 tcggagcagc atagtcaatt gctctgtggg catgaaagct tcttgtaaca ggattaagcc
      901 gattataact gaaaccggag ctgatgtatt tatatgaaag aggggatttt aaaaatattt
      961 tttgcagcga atttccttcc atatcgtaat atcccgcctt gctgttttct gacgcaaaat
     1021 agattccttt atattcggcg ccgtcattta taaatttggc ggctagaatg tttcccggtc
     1081 ccatatagtt tccgtttagc atcctttttt catagacgag tttaaacgag tcttctactc
     1141 gtatgtctac cgcgaaatct atctgccagg cgaaaacttc tgagagagca agggcaattc
     1201 tttcatctcc tcctgaattg attatcgttt caaaaagaga agattcgatt gtgccttcta
     1261 tctcctcggc ttctatttcg tatgggatgg cggttctctc tgcggtccac gtgttgtcag
     1321 ttgttttttg caccgcaagt atttcgtctg cgtttatttg atagattact ttattaaaaa
     1381 ctccagtttc tctatcggac acgaagctta tctgccgtcc ctccacaatt ttcgctaagt
     1441 tatatttttc ttttgaagct tcaagtatag acagggtttc ttctggactt actccaattt
     1501 cttcagccac aagcgcaaaa accgttccgg ccgctatttt tctgttggtc atcttttccg
     1561 tttcgtcggt gaacaagctt ccaatccacg gagaaccgat tgtggcggtt atggctcccg
     1621 catctactcc ctttcccaga ccagtattat tgcttaacca taaaatcgag ctagctacta
     1681 ttataaggag cccgaaaaaa acgataaata tcttttgcat aatcctctat gattttagat
     1741 gaatatcctg tataggcaaa ttaattttgt atttcttttt cagccagtcc tctgagcaat
     1801 gttttttggg attcaattct tttggagaat aagccgacca agaacggcga aactatgctt
     1861 gttacgatac tcaaaagcac gaccgatgtt aatagctctt gagttaaaat tccttggtta
     1921 aatcctaaga aggcgactgc gagtgtggtg gagagtgatg gaattgtaga tgctcccaag
     1981 aacgcgcttt ctccggcggt gaatcctaga atttttccgc ttaaccatcc gcttataaat
     2041 tttgagccga ttgagccgat gaccaccgcg atcgctattg ggaatatgag ggttgcgtct
     2101 tgaagtacgc taagattaat atttgatccg ataaccacaa agaaaagggg gatgaataac
     2161 ccgtaactta ctgcgtggag ttttgctctg gcgatttttg tctgcactga acctgataag
     2221 ataaatccag cgaagaagcc ggcgataatg gcgtgtaatc ctaggaattc gaagagtacg
     2281 acaagtccta tcaaaataag gaggacaaaa cgcaattcac gctcaaataa atcttcttct
     2341 tctgcgtatt ctccaaacgc ctggcgaatt ttaggtaaga tccacgctag tgctccgagg
     2401 aaaagaatta agagagggta gagtaatatg ataaggaaat tataatctcc tgctattgac
     2461 tgaagaaaaa gagataggag tattagactt gcgatatcaa ttagaatggt cgccccgatt
     2521 atcattttgc cgaattgaga gctgatgatc ccggcagact gaaaagaagg gatgagaagc
     2581 gcgattgacg atgacataaa tatgattccg agaagtatcg ccgttggcca agcgtatcct
     2641 aagccgagtc cgattgcgac gccgacgagc gcgggtaacc ctccgctaag aagggctaca
     2701 actccgattt ttttcttgat tccttttgta tcggagaatt tggtttcaag tccggccata
     2761 aacatcagaa ataccaatcc tattgctgcc aaaaaatcta ttgttgcgtt cgcctcaaaa
     2821 aattttagac catgtggccc gattatgaac ccgccgataa taagcgcaac cacccaaggc
     2881 acccgtattc ttcttatcag gctggagaag aggactgcgg ttaaaagtat gataaagaat
     2941 gaaaagaatt gtggcatata tttataatat taaccctgga aggcgaagat aaaacatctt
     3001 tatttgtatt cggcgataca ggcgtttaat tcttccgtcg gtgtccaccc acattcgcca
     3061 tttttctgcc tttcacatct ggcattttga tagcaggcgt actctgggag gtattcgcaa
     3121 gtggtaatta catcttcatc ggaacagatg tgattactgc accctgtcgg gcggcattcg
     3181 gtgagttctg taccgttttc atcaatggct ttctcccagc cgatatcata cccgaaataa
     3241 tagccgatac ctactgccag aatcaccatc acaattattc ccaatataag tttcatatac
     3301 acgattacaa cttggtaatc ccgttaccaa cttctgtatg acaattttta cataatatta
     3361 tacactttcg cgcctcttct agtatagtgc tccatccata acttaaccct ttgacgctaa
     3421 gagaaaaaga tttgttttta ggatcaaggt gatgaaaatc taaggctccg atatattttc
     3481 tataaccgca caatttgcat tttccgccga ataaatcaac tagcctcttt ttcttcttcc
     3541 tgcgactagc gatcacccaa ctcgatgcac actttccgca acggaataaa ccattctccc
     3601 tttcaaaatg attggttctc ccatgcttga aacacttctt aatcactatt ctattatatc
     3661 acggcgtaac accaaaagtt aagctaggca atgataattt agtctcatca tggatacctt
     3721 gtttttttat acgcgttatt ctaaact
//
LOCUS       DATDNZ010000077        17211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3095194, whole genome shotgun sequence.
ACCESSION   DATDNZ010000077 DATDNZ010000000
VERSION     DATDNZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095194"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VI432_02915"
     CDS             complement(<1..505)
                     /locus_tag="VI432_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497074.1"
                     /translation="MNSKTLLWTTLVILNFALAGCVENPMKDYYESDDSSSEVSTTDE
                     TETNSEEQTSSKENNSPEDPPPEEANHGGGTENEGSPEEPTPEGELPVLEIGVEATNL
                     RREVDGYSATTGMFTFSAEVAQDGPSINWLLDSENIGIGSQISHELTEPGNYLIKALL
                     ETATGDTI"
     gene            complement(748..1629)
                     /gene="pyrF"
                     /locus_tag="VI432_02920"
     CDS             complement(748..1629)
                     /gene="pyrF"
                     /locus_tag="VI432_02920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY4497075.1"
                     /translation="MKRNARQKIVVALDTDDPRRALSLVAQLRSEVGAFKFGKEFMSG
                     TLRRIISLPKQEAFECVGALRALFNELDGNVFWDGKFNDIPNTVSKATGEIAALGVKM
                     LNVHCTGGEEMMREAALVAKRMSNANGKKRPLVLGVTLLTSLSYQDLVKMGLAPDVRT
                     PGNQNVEKEKQKIGRERIVQTLALLAKDCGLDGVVASAKEAPIIRRACGEEFKIVTPA
                     IRPEWALPNDQNKDRITTPTQAIRAGADMLVIGRPITNPPPGIGSPIEAARSIQAEIE
                     FALGERRGQEEKITLAD"
     gene            complement(1738..2160)
                     /locus_tag="VI432_02925"
     CDS             complement(1738..2160)
                     /locus_tag="VI432_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497076.1"
                     /translation="MTLSQTLTDLAKMAGVECPSGIPKELDYRFITGVTAVFQFVKFD
                     GLRDSERVLVTLSNFHMKPANVHQLLALFIEHRAAIIRLAVRNGKIDFHAVAMDSIFE
                     GEVPVLSIENGPPELNMEPTTSEWTTSHRFPVMRTRKL"
     gene            complement(2710..2862)
                     /locus_tag="VI432_02930"
     CDS             complement(2710..2862)
                     /locus_tag="VI432_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497077.1"
                     /translation="MGMFGDIARENVREGIMPVIERLLRENESKPEAIKAIEEIKDAI
                     LKATAT"
     gene            complement(2866..3519)
                     /locus_tag="VI432_02935"
     CDS             complement(2866..3519)
                     /locus_tag="VI432_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HEY4497078.1"
                     /translation="MTTTHNELKKRWNELMKSLGVNDARGEIFNDLVARYSEPHRRYH
                     TLEHIVNMLREFAKVKAHASYPKAVEFAIWFHDAIYDTRREDNEQASAKLAVEAVKKL
                     GITNGVFGDIFSPKVYELVLTTEYKRLPGDADEDLLADLDLATLASDPEIFDENMKKI
                     REEYSWLSDEQFKWGRIMFMRNMLSRPRIYSSPRTREKYDARARANLERSIKKLKEE"
     gene            complement(3572..3763)
                     /locus_tag="VI432_02940"
     CDS             complement(3572..3763)
                     /locus_tag="VI432_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497079.1"
                     /translation="MGMFGPREGSWWLRSKKDPRWNTDGRAYVGGFLMPEACRSKIDE
                     LKLRFGEPPEDLEWGYMKD"
     gene            3889..4650
                     /locus_tag="VI432_02945"
     CDS             3889..4650
                     /locus_tag="VI432_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4497080.1"
                     /translation="MKQLHQILGKLDFSDAESKVYIALAQGSASAREVMKVTRQKRPT
                     VYYALNSLEKKGLISRSSPGNKGEFVVESIDRLKSLIKEKYLEIERLSKDVESVTPFL
                     NDKISKDKKPAVMFFEGVDAVKNVVMGSLYCRDKKILSIAPKDNFFWQFSRDFVEAYV
                     SERVRNRIETRNLWEERIEESIYREYYEGVSDVRILPGVMHNKFKTTVFIYDNKTLYI
                     SSIKDSYAVLITSKEHNDMMRAVFDGLWIGSEKHS"
     gene            complement(4700..5266)
                     /locus_tag="VI432_02950"
     CDS             complement(4700..5266)
                     /locus_tag="VI432_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497081.1"
                     /translation="MTNNENCSFIPGRGNRMDGQKSEEYRNHTKLFIIIALVGTLVFS
                     VWATVRIVEYVDYQEEIGGHINRAKIANTVPTAIQEMRIVVRELEEHGYTSGCTCIFF
                     NFPENDIGFWYNNLKESLQELEDLPPNASTLETSNTLLKLRETLEKAPNGITIYPHNT
                     AFFSWGTLSALWGGGFWILLFGRYMKNL"
     gene            complement(5333..5596)
                     /locus_tag="VI432_02955"
     CDS             complement(5333..5596)
                     /locus_tag="VI432_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4497082.1"
                     /translation="MYFVYMARNLRDKLYIGISENLSKRIKYHNTKRGAKFTNGGNFE
                     IVFIEKYQTLEQARRREIQIEKWRREKKELLIGRHSKGSETKI"
     gene            complement(5728..6050)
                     /gene="ssrA"
                     /locus_tag="VI432_02960"
     tmRNA           complement(5728..6050)
                     /gene="ssrA"
                     /locus_tag="VI432_02960"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6106..6549)
                     /gene="smpB"
                     /locus_tag="VI432_02965"
     CDS             complement(6106..6549)
                     /gene="smpB"
                     /locus_tag="VI432_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4497083.1"
                     /translation="MPEIAKNKRAYFDYNIIETYEAGIELLGLEVKSARNGGLNLAGS
                     YVIIRGNEAWLLNADLRPYQPMNTPSDYDSKRTRRLLLNNKEIKELISRSKESNLTIV
                     PLRATIKGRFIKLEIGLAKSKKKTDKREAIKKRESDRDIKKRFKV"
     gene            complement(6557..7207)
                     /locus_tag="VI432_02970"
     CDS             complement(6557..7207)
                     /locus_tag="VI432_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY4497084.1"
                     /translation="MNNIKAVIFDVDGTLTERLSWLFLTEKLGASTDKHAEMLNGLRS
                     GKYSLSQATKQLVNLWKDTGNANKSSFRKTFDSVPLRSESIDIINYLKNRGYLLCLIT
                     GSMDLYAKIIADKLGVQKYFWNTDLIWDNENELSSFNYEPDQDTKKLAQLDEFINSIK
                     ISTEECAVVGDSKNDVKLFQETKHGVYLDSGIENAPELEKIAWKKIKNLKELTAIL"
     gene            7271..7867
                     /locus_tag="VI432_02975"
     CDS             7271..7867
                     /locus_tag="VI432_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4497085.1"
                     /translation="MDKQSLTNITHFLFEAGMLAKTPRSGFFFLGSGEQSVAEHLNRT
                     AYVGFVLASMNKKADVSKVMQMCLFHDFTESRISDLNYVHQKYTERHENKALADLVGS
                     IPFGKYIDALIKEYTARETIESKLAKDADNIEFLLSIKEQIDIGNVRGKKWIPSLLKR
                     LKTEEAQELAKSIINTESDRWWIGDENDEWWVSRNKKN"
     gene            7870..8151
                     /locus_tag="VI432_02980"
     CDS             7870..8151
                     /locus_tag="VI432_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEY4497086.1"
                     /translation="MLEILTTIMGVMMSLAYYPQAYKIYKNKSAKNVSISSFIIFSVG
                     TATWLAYGIYLKDAPLVISFVFGVLGSWLVLVLSIVYRDNKNIDQNILS"
     gene            8228..8569
                     /locus_tag="VI432_02985"
     CDS             8228..8569
                     /locus_tag="VI432_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497087.1"
                     /translation="MASKLVEAIIKTHGVEHASGCVVEQGEIGRSAMMGRFGRLFTQG
                     HVVVILNTRDQIFVLNDPRQILSVDFNGFNIYQAVSIPTDIWEEVTKELGNPPRIVDA
                     RFGRVNALLRG"
     gene            8643..10349
                     /gene="argS"
                     /locus_tag="VI432_02990"
     CDS             8643..10349
                     /gene="argS"
                     /locus_tag="VI432_02990"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY4497088.1"
                     /translation="MQRAFLEEFVKKEFKLEDNFLFGPPEIKSHGHLSTNAAFALAKI
                     KKISPMAAGELVRDLVSKKFTNEFSKIDVIAPGFVNFWFSDESIGRQFKNIYKNREKL
                     GKPESSVLKYNKTKKVIVEYPSPNIGKPMHVGHMRSTIIGDAIANIHDFLGYKVIRWN
                     YIGDWGTQFGKLIYAYKRWGNKVDLRKNPITSLLGLYIKFHNEAKKDESLDKEGQTEF
                     KKLEEGNLENRRLWQLFTKESLKELNKIFKTLDIKFDVTLGESFYEEKLKPLINEFLE
                     KGIAMRSEGSLIINLNKFNLQPALIQKSDGASLYLTREIANLEYRVKKYKPAKILYVV
                     GNEQSLHFEQLFAIAKILGLDESELSHIKHGLVLGEDGKKLSTREGRIVVFEDLIKKS
                     IELAYLAADTKNPKLPQKDKKKISEIVGIGALKYNDLSQNRNSNIGFDWDKMLSFEGN
                     SGPYIQYTYVRMKNILNKARKMKLFNVKQLSREIDQELILKLFNFGDVVERAGEENMP
                     NYIAQYLYDLAQIMNKFYEAEPIIKSEKEIRDARLALVEASAKTLKNGLNLLGIKTLE
                     KM"
     gene            10413..13886
                     /locus_tag="VI432_02995"
     CDS             10413..13886
                     /locus_tag="VI432_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4497089.1"
                     /translation="MLKNTEEFSLPKLEKQILDSWKKNDIFRKTLENRSKRKTFNFYE
                     GPPTANGKPGLHHVLARSFKDIVLRYKTMDGFFVPRKGGWDTHGLPVELQVEKRLGLK
                     SKKDIEKYGIEKFNKKCRESVWEYKTEWENITERMGFWLDLKNPYITYEPSYMETLWW
                     IISQAWKKKLLYKGHKVVPWCVRCGTALSSHEVAQGYKEVNDKSVFIKFKLRKNQKVG
                     RQFITNDKTYILSWTTTPWTLPGNVALAVGKDINYLAVSHGEGGDAEIWILAKDIYMK
                     EEIFKGLGEIKVLYELTGKELLKLSYEQLFSVKTLKNNKSHKIYDADFVTTVDGTGVV
                     HTAVMYGDDDYKLGVKNGLPQHHTVDENGKFSSDVPILRGKYVRSEETNQKIIGYLVK
                     NGYLLKTESYKHDYPFCWRCDTPLLYYAKDSWFIAMSKLRDRLLESNSRVNWVPETVG
                     RGRFGEWIKDVKDWAISRARYWGTPLPIWECKNGHIKVVGQIEDLGLSSGNKFFILRH
                     GEAEHNVRDIIASGAESGGKLSHLTKKGIKEAKLAGEDLNRKKIDLIISSPYKRTMDT
                     AKIISEITGAKVLRDQGLKEIDLGKFNWKKVDQYRNFFKNKFDRFDVAPQGGENLNDV
                     TKRVVKTIIKIDSKYRNKNILIVGHGDPLWMLEKSLTGTSNEGSFEMAYLKPGEWKEI
                     KLLNTPYDNNGNIDLHRPFIDKIILDCDKCKEKMARVKDVVDVWFDSGAMPFASWHYP
                     KEYKDLIDDRKKYPADYIVEGMDQTRGWFYTLLAVATILDNDEPYRNVISLGLIHDKF
                     GQKMSKSKGNVVDPWAIIEKYGVDAVRWYFYTVNPPGEQKNFDEADLGKVSRKIFSIL
                     YNSFVFYNTSEKSGYEEKQEEKHVLDRWVIARLHQTIKSTTSYLDGFEIGLAAREIEG
                     FIDDLSRWYIRRSRKRPEMASTLKVTLLEVSKLMAPFSPFFADALYKSVGGIKESVHM
                     EDWPKSQKDLIDKELIVGMEQVRKISTSILAKRSEAGIKVRQPLGLLKVNLSEDGKGF
                     KVEDKELLDVLREEINVKNVVLDKNIDGEFVLDTEITPALKEEGMAREITRIIQGLRQ
                     EANLKPKDRIDVFIKTESQVVSSIVVRWDKEIKEVTGADRVRLEKSDKFKVETETHLE
                     GESLWVAIKKV"
     gene            13924..14907
                     /gene="trpS"
                     /locus_tag="VI432_03000"
     CDS             13924..14907
                     /gene="trpS"
                     /locus_tag="VI432_03000"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY4497090.1"
                     /translation="MTKPIVISGIKPSGELHIGNYLGMLRNALALQESNKYNCFYFIA
                     DYHAMTQKYDPKDLREQTISVTADLLATGINPKKSAIFIQSHVPEHANLAWIFNTITT
                     VSQLERMVEYKEKLGDGQVPNTGLFDYPVLMAADILVYKSRRVPVGEDQRQHLELARD
                     IARTFNGRFGETFKEPKAVLTRIPRVMSLNNPSKKMSKSIPSGCLYLQDSPKAIREKV
                     MSAETDSMREIGYDPATRLGVSNLVLIYSEFSGDTPDEVVNKFTGKGYREFKESLADL
                     IIEKLADFQEKRKKILKDKRAIIKILESGEKRVSPIAEKTMEEVRRKTGLI"
     gene            14942..15301
                     /locus_tag="VI432_03005"
     CDS             14942..15301
                     /locus_tag="VI432_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4497091.1"
                     /translation="MKSYKELIVWQKSVELVEEIYKLTPFFPKDEIYGISSQMKRAVV
                     SIPSNIAEGFVRKHNKEFQQFIRTAFGSAAELETQLIIAKKLRLAPSNNFNKIEALLN
                     EILKMLNRLISSLSIDH"
     gene            complement(15319..17103)
                     /locus_tag="VI432_03010"
     CDS             complement(15319..17103)
                     /locus_tag="VI432_03010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY4497092.1"
                     /translation="MQTRTHRRTQASSSGRYRTSKIMSEESKYPHKKPKQKHPASDDV
                     VRLVPLGGLEEVGRNMAFVEYKDEIVIIDMGIQFPEEETPGIDFIIPNIEYLIPKKQN
                     IKGIVLTHGHLDHVAAIPYLIGKLGNPTIYTTKLTKAIIERRQEEFVNAPKLIVEVVE
                     NGTKVKLGKYLEIEIFGVDHTIPDTTGVVIKTPVLNIVHFADFRVDYNEAGEPEKFDE
                     FKRIGELGIHTFMVDSTNAEEEGQSLPEKTVEKNLEILFKKAEGRIILSTFSSMLTRI
                     AEIIKIAEKLDKKVVINGRSMKDNIQIAQNLGYMKFKKGTIISVEEAHKHKDEKILVI
                     TTGAQGESTAGLMKIVNGEHKYINIKAGDTVIFSSSVIPGNERSVQNLKDNLSRQGAI
                     VYTSKMIDIHSSGHAPKEDIKKVIQLIKPKFLVPVHGYYFMRGANGQNGIDAGMPKEN
                     IRLMDNGQVGSLTKDSFIITDETVPAHYIMVDGLGVGDVGEVVLRDRRILAQEGMIVV
                     ITTIDRKTGRILKNPDIISRGFIYLKDNQYLLEEVRKKIRGIIGRIPKHQPLDADYLK
                     NLFRDQIGQYLYNKTFRRPMVLPVIIEI"
     gene            complement(17129..17211)
                     /locus_tag="VI432_03015"
     tRNA            complement(17129..17211)
                     /locus_tag="VI432_03015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17176..17178),aa:Leu,seq:cag)
BASE COUNT         4929 a         3188 c         3757 g         5337 t
ORIGIN      
        1 agatagtgtc gccggtcgcg gtttctagga gcgctttgat caaatagttt ccaggttcgg
       61 tgagttcgtg ggagatctgg ctgcctatcc caatattctc cgaatccaaa agccagttga
      121 tcgatggtcc gtcttgcgca acttccgcgc tgaaggtgaa catccccgtt gtagccgagt
      181 aaccatctac ctctcttctg agatttgtgg cctcgacgcc aatttccaat accgggagtt
      241 cgccttcggg cgtgggttct tcgggcgatc cttcgttttc tgtgcctcct ccatgattag
      301 cttcttctgg cggaggatct tccggagagt tgttctcttt cgaggatgtt tgctcttcgg
      361 aattcgtctc tgtttcgtct gttgtgctga cctcggaaga agaatcatcg gattcgtagt
      421 aatccttcat tggattttct acgcatccgg cgagggcgaa gttaaggatg acaagcgttg
      481 tccagagtag tgttttgctg ttcataggag ttccgtactc cgcaaatgcc gtataggagc
      541 ggtctttgcg gaatacggaa ccgaatatta aagagcttat tttgattttg accctcttca
      601 taacactttg tcaaattacc attatatccc gcgtgtttaa ttcatccccc ttttatcccc
      661 atggggataa aagggggatg aagattctga cccaaatttt attgagaata aaaaaaccag
      721 tcctaaattt gggctggttg tttgtttcta atccgcaaga gtaatttttt cttcttgtcc
      781 tcttcgctct ccaagggcga attcaatctc tgcttgaatg ctcctcgcgg cttcgattgg
      841 gctccctatt cccggtggcg ggttggtgat gggtctgccg ataacaagca tgtctgcgcc
      901 ggctcgtatt gcttgtgtgg gcgttgtgat cctgtctttg ttttgatcat tgggcagagc
      961 ccattcaggt cttatggctg gagtcacaat cttaaattct tctccacaag cccgacgaat
     1021 aatcggggct tcttttgctg atgcaacgac gccgtctagt ccgcagtctt ttgcgagtag
     1081 cgcaagagtt tgcacaattc tttctcttcc gattttttgt ttttcttttt caacgttttg
     1141 gttgcccgga gtcctcacgt cgggcgcaag acccatttta accaaatctt gatatgaaag
     1201 actcgtaaga agagttacgc ccaataccaa tgggcgcttt ttcccgttcg cgttagacat
     1261 tcttttcgca acaagagcag cttcccgcat catttcttcg ccgccagtgc agtgcacgtt
     1321 taacatcttt acgccgagcg cggctatttc tccagtcgct ttagagactg tgttgggtat
     1381 gtcgttaaac tttccgtccc aaaaaacgtt gccgtccaat tcattgaata gagctcttaa
     1441 tgcgcctacg cattcaaacg cttcctgctt aggaagtgaa attatacgcc ttagcgtgcc
     1501 agacataaat tctttaccga acttgaatgc ccctacttcg cttctgagtt gggcaactaa
     1561 tgataaggct ctgcgcggat catccgtgtc caacgcgaca acaatctttt gtcttgcgtt
     1621 tcgcttcatt atttcacctc attgttaaag ataactgcac caacagtaca ctttttgaga
     1681 ttggttgtaa ataagattta aagagaataa aaaagcagat tgaggtatgt caatctgcta
     1741 caattttcta gtgcgcatta cgggaaacct gtggctcgta gtccactctg aggttgtcgg
     1801 ttccatgttg agttccggcg gcccgttttc gatcgaaaga acagggactt cgccttcaaa
     1861 aatggaatcc atggccacag cgtgaaagtc tattttgcca tttctcacag ctaagcgtat
     1921 gatagccgct ctgtgttcaa tgaagagagc gagcagttga tgaacgtttg ccggtttcat
     1981 gtgaaaattg gagagggtta ccagcactct ttcagaatct cgcaagccat cgaatttaac
     2041 aaattggaac acggccgtta cacccgtgat aaaccgatag tctaattcct tggggatgcc
     2101 cgatgggcac tctacgccgg ccattttagc cagatcggtt agggtttgtg atagagtcat
     2161 cacaacaggg aattcatttg tggcgcgtgt cattcgatca cccgtcttta atgatctatt
     2221 atagaggttc tcatatgaat cggttctatg tcaataaaat gctttttaaa aattataaaa
     2281 tccatctatt aaagatggac tagtgtttct actgtttgga ttggatttcg agcgttccgg
     2341 ttctcttttc gaggttgtag tgcgccgtgt acggcttttt cgcgccggat tctttcgggt
     2401 cttcggcgag tcctcggagc gtcattagat tgtgatcttc gtcggacggt ctgacggatt
     2461 caatcgttgc tcggatgatt ccgtttcggg taaggaaatc cactccggat ttttggttga
     2521 aggccttgct gatctcttct cttgaggggc cgtttttaat ttttaaatcc tttcgttttt
     2581 gagctatgtt gtgaatgtgc gttgcctcct tttaatggct gctatcagtg tggatttaaa
     2641 ttattaatca gtcaagattt tcgaaataaa aatagaccac ttgttagtgg tcttgttgtt
     2701 ggcagtttgc tatgttgcgg ttgctttgag gatagcatcc ttgatttctt cgattgcttt
     2761 tatcgcctcg ggtttgcttt cattctcgcg gagaagccgt tcgataacgg gcattatgcc
     2821 ttctctcaca ttttctcttg cgatgtctcc gaacattccc atgatctatt cttcctttag
     2881 ctttttgatt gagcgttcta ggttagccct tgcgcgtgcg tcatactttt cccgcgtccg
     2941 cggcgaagag taaatccggg gccggctgag catatttctc ataaacatta ttcggcccca
     3001 tttgaactgc tcatccgaga gccagctgta ttcttcgcga atctttttca tgttttcgtc
     3061 gaatatctct gggtcagatg cgagcgtcgc aagatctaag tcggcaagca gatcttcgtc
     3121 agcatctcca ggcagtcttt tgtattcggt cgtaaggact agctcataga cttttggact
     3181 gaagatgtcg ccgaagaccc cgttcgttat accgagcttc ttcaccgcct ctaccgcgag
     3241 cttcgcgctt gcctgttcgt tgtcttcgcg acgggtgtcg tagatcgcat catggaacca
     3301 aatcgcgaat tcaaccgctt tcgggtaaga agcatgcgct tttaccttgg caaattctcg
     3361 gagcatgttt acgatgtgct ccagcgtatg atatctgcga tgcggttcgc tgtagcgggc
     3421 gaccagatcg ttgaagattt caccgcgggc gtcgtttaca cctaaagatt tcattagttc
     3481 gttccagcgc ttcttgagtt cgttgtgtgt ggttgtcata gatatcccct ttctattatc
     3541 ccagtggtgt tttggggtac gacctttatt atcagtcttt catatatccc cattcaagat
     3601 cctctggcgg ttcaccaaat cttagcttga gttcgtcgat tttgcttctg caggcctccg
     3661 gcattaagaa tccgcctaca taggcacgac cgtcggtgtt ccagcgtgga tcttttttgc
     3721 ttcggagcca ccagcttcct tctcttggtc caaacattcc catgttttca cctcatgttc
     3781 gctaggcctt tctcaattaa acctgattgt taaagagcca gcttcataaa aagattaatc
     3841 gttgatagtt aatatgtata tgctgtataa taacgattag acaacaagat gaaacaactc
     3901 catcaaatac ttggtaaatt agatttttcc gatgccgaat cgaaggttta tatagcgctg
     3961 gcgcaaggtt cggcttctgc gcgagaggtt atgaaagtca ctcgacagaa aaggccaact
     4021 gtttactacg ccctaaactc attggagaag aagggactta ttagtcgctc gtctcccggc
     4081 aataagggcg agtttgtcgt tgaatcaatc gatagattga agagcttaat aaaagagaaa
     4141 tatttagaga ttgaacgtct ttctaaagac gtcgaaagcg taacaccatt tctaaacgat
     4201 aaaatttcta aagataaaaa gccggcggtg atgttttttg agggggtgga tgctgtgaaa
     4261 aatgttgtga tgggatcttt atattgcaga gataaaaaaa tattatccat cgctcctaaa
     4321 gataatttct tctggcaatt cagccgcgat tttgtcgagg cttatgtaag tgaaagagtc
     4381 agaaacagaa ttgagactag aaatttatgg gaggaacgga tagaggaatc catatataga
     4441 gaatattacg agggagtatc ggatgtacgg atcttgcccg gggttatgca taataaattt
     4501 aagaccacgg tttttatata cgataacaag actttatata tttcttcgat caaagacagc
     4561 tatgcggtct taataacctc caaagagcat aatgatatga tgcgcgcggt ttttgacggg
     4621 ttatggatcg gatccgaaaa acactcttaa ataaaaaaga ggccgttgtt ttaggtggcc
     4681 atgacctagt tgtggacact cagaggttct tcatatatct tccgaagagg aggatccaga
     4741 atccgcctcc ccagagtgcc gataaggttc cccatgagaa gaaagcggta ttgtggggat
     4801 agattgtgat gccatttggc gccttttcta gggtctcgcg aagcttcaga agagtgttgc
     4861 ttgtttcgag tgtgcttgcg tttggcggaa gatcctcgag ttcttgaagt gattctttca
     4921 agttgttgta ccagaaacct atatcgtttt ctggaaagtt gaagaagata caggtacatc
     4981 cactagtgta tccatgctcc tcgagttctc ttactacgat tcgcatctct tggattgcgg
     5041 taggtacggt gttggcgatt ttagcgcgat tgatgtgtcc gcctatctct tcttggtagt
     5101 caacatactc tacgatccta acggttgccc agacactgaa gacgagggtg cccacgagcg
     5161 ctatgatgat aaagagcttg gtgtggttcc gatattcttc acttttttga ccatccattc
     5221 ggttacctct gccaggtata aaactacaat tttcattatt tgtcaaacac aaaaatcgcc
     5281 cttaaaggcg gttttggggt gttggtgtag atggcgggag ggtctcacgt cgctagattt
     5341 tagtttcgga tcccttcgag tgtcttccaa taagtaattc cttcttttct cttcgccact
     5401 tttcaatttg gatctctctt cttcttgctt gttcgagagt ttgatatttt tcgataaaaa
     5461 caatttcaaa attaccccca ttagtaaatt tagcgccccg tttagtatta tgatatttaa
     5521 ttctttttga gagattttcg gatataccaa tataaagttt gtctctcaaa ttacgagcca
     5581 tataaacgaa atacatattt aaattatagc ccattcgact cctcacgagt tcgtcgccca
     5641 gggcactcga cttaaacaaa aaacttccga tcggaagttt ttgtattcgc tgtggtttac
     5701 cacgagcgag ccctttatgg gcgagtcgag tggaagtggg aggattgaac ccccgtgcga
     5761 aagtttgatc taaaataatc tacaagctta gttctcttgc gagtacgttt ttgctttgct
     5821 ttatttcact tttgagacgc aaggcaagat cttcaaaagc tatcctaatt tattacacca
     5881 gttgtctact atcaggagtc gtaggctgat ggcttgagtg cttaagcgta tgctaaagct
     5941 ggaactcgag cggtaaaact gtttttagca gttatttttt tgtcggattt acgagttgac
     6001 gctcggcttg catattttag aaaagaacac tcgtcgatgc tgacactccc tcaaaatgca
     6061 ttttatctta aaaaagtaaa ccgcgcttta agtgggtgag gtgaattaca ccttaaatcg
     6121 ttttttaatg tctctatcgc tttcccgttt tttaatcgcc tcgcgcttat cagttttctt
     6181 tttggatttt gcgagcccga tctctaattt gatgaaccta ccttttatag tagcacgcaa
     6241 gggaacaatt gtcaagttag attccttgct cctgcttatc aactctttta tttctttatt
     6301 gtttaaaagg aggcgtcttg ttctctttga atcgtagtcg gatggagtgt tcattggctg
     6361 atagggcctt aagtcggcgt ttaatagcca cgcttcattt ccgcgaataa tgacatagct
     6421 tccggctaga tttagtccgc cgtttcttgc agacttcacc tcaaggccga ggagttcgat
     6481 tccggcctca taagtttcta ttatattgta gtcaaaataa gcgcgcttat ttttagcaat
     6541 ttctggcatg atttatttat agtatagcag taagctcttt caagttttta attttcttcc
     6601 acgcaatttt ttctaattcc ggagcgtttt cgatcccgct atcgagatat acaccgtgtt
     6661 tagtttcttg aaacaacttt acatcgtttt tactgtctcc gactactgcg cattcttctg
     6721 tggaaatttt aatgctattt ataaattcat ccagttgtgc taatttcttc gtatcttggt
     6781 ccggctcata attaaaacta gaaagctcat tttcattatc ccaaattaga tccgtattcc
     6841 agaagtattt ttgaacacca agtttgtcag caattatttt tgcatataaa tccatcgaac
     6901 ccgttattag acagagtagg tagcccctat tctttagata attaattatg tctatagatt
     6961 ctgatctgag cggaactgaa tcaaatgttt ttcgaaatga gctcttattc gcattacccg
     7021 tatccttcca taaattaaca agttgttttg tagcttgcga gagagaatat tttcctgatc
     7081 taagtccgtt tagcatttct gcgtgtttat cggttgatgc gccgagtttt tcggtcaaga
     7141 aaagccaaga taaacgctcg gttagagtcc cgtctacatc gaaaataaca gccttgatat
     7201 tgttcatggt tagatattgt attttctttt tacccgcttt tagagttatt atcaggatat
     7261 atttaagttt atggataagc aaagtttaac caacataacc cattttttat ttgaagctgg
     7321 gatgctcgct aaaactccta gaagcggatt tttctttctt ggaagcggtg agcagagtgt
     7381 ggcagaacat ttaaatagga ccgcttatgt tgggtttgtt ttagcttcga tgaataaaaa
     7441 agcggatgtt tctaaagtta tgcagatgtg tttattccat gactttaccg aatcaagaat
     7501 ttcggatttg aattatgtcc accaaaaata taccgaacgc cacgagaata aagctttagc
     7561 tgatcttgtc ggatcaattc cgttcggtaa atatatagac gccttaataa aagaatatac
     7621 agcgcgagag acgatcgagt ctaagcttgc gaaagacgct gacaacatag aatttttgct
     7681 ttccattaaa gagcagattg atatagggaa tgtaaggggc aagaaatgga taccttctct
     7741 tctcaaaaga ttaaagactg aagaggctca agaattggct aagtctatta taaatacgga
     7801 atcagacaga tggtggatag gagatgaaaa tgacgaatgg tgggtgagca gaaataagaa
     7861 aaattaatta tgttggaaat tttgacaacg attatggggg ttatgatgtc tctagcatat
     7921 tacccccaag cttataaaat ttataaaaat aaatcagcta agaatgtttc tataagctct
     7981 ttcataattt ttagcgttgg tacggcgact tggcttgcat atggtattta tttaaaagac
     8041 gcgccattag ttataagttt tgtattcgga gttttgggat cttggttggt gttagttcta
     8101 tctatagttt atagagacaa taaaaacatt gaccaaaata ttctgtcgtg atattttaaa
     8161 aatataaatc aaatttcttt ctctgatttt ttccgctctt tcaaaataaa aatgaggtga
     8221 gagcaatatg gcatctaaac ttgtcgaagc gatcataaag acgcatggcg tagagcatgc
     8281 gtcgggatgc gtggttgagc aaggggagat cggacgatca gccatgatgg gcagattcgg
     8341 ccggttattt acccaaggcc acgtcgtcgt aatcctaaat accagggacc agatttttgt
     8401 cttaaatgat cccaggcaga ttctaagcgt agatttcaat ggatttaata tttatcaagc
     8461 ggtcagtatc ccgactgata tttgggaaga agtgactaaa gagctaggta atccgccgag
     8521 aatcgttgat gctcgttttg gcagagttaa cgccttgctt cgtgggtgaa atccgaatcc
     8581 gtctgcgctg caggcggctt tcttattaaa tggaaaatat attcacatgc tatactagtc
     8641 acatgcagag agcttttctt gaggagtttg ttaagaaaga atttaaactg gaagataatt
     8701 ttttattcgg tccaccggag attaaatccc atggtcattt aagcaccaac gccgcttttg
     8761 cgcttgcgaa aattaagaaa atttctccaa tggctgcagg cgagcttgtg agagatcttg
     8821 tcagtaaaaa atttacgaac gagttttcta aaattgatgt aattgctccg gggtttgtta
     8881 atttttggtt cagcgatgaa tcaataggaa gacaattcaa gaatatttat aaaaatagag
     8941 aaaaattggg caagccggaa tcgtctgttc tgaaatacaa caaaactaaa aaggttatag
     9001 ttgaataccc gtcgcctaac attggtaaac cgatgcatgt tggtcatatg cgatcgacta
     9061 ttatcggaga tgcgattgcg aacattcacg attttttagg atacaaagta attcgctgga
     9121 attatatcgg agactgggga acgcagttcg gcaaattaat ctatgcctat aaaaggtggg
     9181 gaaataaagt tgatttgaga aaaaatccta tcacttctct tttagggctt tatattaaat
     9241 ttcataatga agcgaagaaa gatgaatcat tagataaaga aggccagaca gaattcaaga
     9301 agcttgaaga aggcaatctg gagaacagaa gattgtggca gttattcacc aaagaatcat
     9361 taaaagaatt aaacaaaata ttcaaaacac ttgatataaa atttgatgta acgcttggcg
     9421 agagttttta tgaggagaaa ttaaaaccat taattaatga atttctggag aagggcatag
     9481 ctatgagaag cgagggctcg ctcattataa atttaaacaa atttaatctc cagccggctc
     9541 ttattcaaaa atcagatgga gcaagtttgt accttactcg tgagattgca aatcttgaat
     9601 atagagtaaa aaaatataaa ccggcaaaaa ttttatacgt tgtgggaaat gagcaatctt
     9661 tgcatttcga gcaacttttc gcgatcgcaa aaattttagg tctggacgaa tcggaactct
     9721 ctcatattaa gcatggtttg gttttgggtg aagacggcaa gaagctctct actagagagg
     9781 ggagaattgt ggtttttgaa gatttgatca agaagagtat tgaacttgct tatttggcag
     9841 ccgacactaa aaaccccaaa ttaccacaaa aagataagaa aaaaatatct gaaatagttg
     9901 gtataggcgc tcttaaatat aatgacttat cgcaaaatag aaatagtaat atcggttttg
     9961 attgggataa gatgttaagt tttgagggga actctggtcc atacatccaa tatacgtacg
    10021 tccgaatgaa aaacattcta aataaagcaa gaaaaatgaa attgtttaac gttaaacaat
    10081 tgtctcgtga gatagatcag gagcttatat taaagttgtt taattttgga gatgtggttg
    10141 aaagagctgg tgaagagaat atgcctaatt atatagccca atatttatac gatttagcgc
    10201 agataatgaa taagttttat gaagcggagc cgattataaa gtctgaaaaa gagattcgcg
    10261 atgcgcgtct tgctcttgtt gaggcttcag caaagacgct taaaaatggg cttaatcttt
    10321 taggaataaa gaccttggag aaaatgtaag ctcaaaatga gcgattttta aatagctgtt
    10381 tttgtgataa tattcaagat accttatata aaatgcttaa aaatactgag gaattttctc
    10441 ttcccaagct tgaaaagcaa atactggatt cctggaaaaa gaacgacatt tttagaaaaa
    10501 cgctagagaa tagatctaaa cgcaaaacgt ttaattttta tgaaggtccg ccgaccgcaa
    10561 atggcaagcc cggcttgcat catgttcttg caaggtcttt taaggatata gttcttcgtt
    10621 acaaaacgat ggacggtttt tttgtgccca gaaaaggtgg atgggatacg catggtctcc
    10681 cggttgaact tcaagttgag aaacggttag gacttaaatc taaaaaagac attgagaagt
    10741 acggcataga aaagttcaat aagaagtgca gagagtctgt ttgggaatat aaaacggagt
    10801 gggaaaatat tactgaaaga atgggttttt ggcttgattt gaaaaacccc tatattactt
    10861 atgagccgtc atatatggaa acgctctggt ggataatctc gcaagcgtgg aagaaaaagc
    10921 ttttatataa aggtcataaa gttgtgccgt ggtgtgtcag atgcggaacc gcgctttcat
    10981 cccatgaagt ggctcagggc tataaagagg taaacgataa gtcggttttc ataaaattta
    11041 aacttagaaa aaatcagaag gttggcaggc agtttattac aaacgataaa acatatattt
    11101 tgtcttggac gacaactcct tggacgcttc caggcaacgt tgctttagcc gtcggcaagg
    11161 acatcaatta tctagctgtt tcacatggag aaggaggcga tgcagaaata tggatactgg
    11221 ctaaagatat ttatatgaaa gaagaaatat ttaaagggct tggcgagatt aaggttctat
    11281 atgaattaac tggaaaagag cttttaaaat tgtcttatga gcaattgttt tcggtcaaaa
    11341 ctcttaaaaa taataaatcg cataagatat atgacgctga ttttgtgaca acggttgatg
    11401 gaacaggtgt ggtccatacg gctgttatgt atggagatga tgattataaa ttgggagtta
    11461 aaaatggatt acctcaacac cacacagttg atgagaatgg aaaatttagt tcggatgttc
    11521 ctattttgag ggggaaatat gtgaggtctg aagaaactaa tcagaagata atcggatatt
    11581 tagtaaaaaa tggatatctt ctaaaaaccg aaagctataa acatgactat ccgttttgct
    11641 ggagatgcga cacccctctt ctctattatg ctaaagactc ttggtttata gctatgagta
    11701 agttaagaga tagactgctt gagtcaaata gccgtgttaa ttgggttcca gagactgtgg
    11761 gccgtggacg cttcggtgaa tggattaaag atgttaagga ctgggcgata tcgcgcgcgc
    11821 gttattgggg cactcctctt cctatttggg aatgtaaaaa tggccatatt aaagttgtgg
    11881 gccagattga agatttgggc ctgtcttcgg gtaataagtt ttttattctt cgtcatggcg
    11941 aagcggaaca taacgtgaga gatattattg cttcgggtgc ggaatccgga ggcaaattat
    12001 cccatctcac aaaaaaagga attaaagaag ctaagcttgc cggggaagat cttaatcgca
    12061 agaaaataga tttgataata agttcacctt ataaaagaac gatggatacc gcgaagataa
    12121 tatctgagat caccggcgca aaagttttaa gagatcaagg attaaaagag atagatttgg
    12181 gtaaatttaa ttggaagaaa gttgatcaat atcgcaattt tttcaagaat aaatttgatc
    12241 gttttgatgt tgctcctcaa ggcggagaga accttaacga tgttacaaag agagttgtaa
    12301 aaacaattat taaaattgat tcaaaatata gaaataaaaa tatattaatt gtcggacacg
    12361 gcgatccatt atggatgctt gaaaaatctt taacagggac ttctaacgag ggcagttttg
    12421 aaatggccta tcttaagccc ggcgagtgga aggaaataaa acttttaaat actccttacg
    12481 ataacaatgg caatattgat ttgcataggc cgtttatcga taagataatt ttagactgcg
    12541 ataaatgcaa agagaaaatg gcgagagtga aggatgtggt tgatgtttgg tttgattcag
    12601 gagcgatgcc ttttgcttcg tggcattacc caaaagaata caaagatttg atagatgaca
    12661 ggaagaaata tccggccgat tatatagttg agggaatgga tcagacgcgc ggatggtttt
    12721 acacgctttt ggctgttgca actattttag acaacgatga gccatataga aatgtgattt
    12781 ctttgggtct tatccatgat aaattcggac agaagatgtc taaatcaaaa ggcaatgtcg
    12841 ttgatccatg ggcaataata gaaaaatatg gcgttgatgc ggtcaggtgg tatttttaca
    12901 ctgttaatcc gcccggagag cagaaaaatt ttgacgaagc cgatcttggg aaagtctcca
    12961 gaaaaatatt ttctatcctt tataattctt ttgttttcta taacacatct gaaaagtctg
    13021 gttatgaaga aaaacaagaa gaaaaacatg ttttagacag gtgggttatt gccagacttc
    13081 atcaaacgat taaatcaaca acctcttatc tagatggttt tgaaataggt cttgcggcaa
    13141 gagagataga aggctttatt gacgatttgt ctcgctggta tataaggcga agcagaaaaa
    13201 ggcccgaaat ggcaagcact cttaaagtaa cgcttcttga agtaagtaag ctaatggccc
    13261 cgttctctcc attttttgca gacgctcttt ataagtcagt cggaggaatt aaggaatcgg
    13321 ttcatatgga ggattggcca aaaagtcaga aggatttaat agataaagag ttgattgtgg
    13381 gcatggagca agtaagaaaa atatccacat caattttagc taaaagatct gaggcaggaa
    13441 taaaagtcag gcaaccgttg ggattgttga aagttaattt atcagaggat ggtaaaggtt
    13501 ttaaagtcga ggataaggag cttttagacg ttttgcggga agagattaat gttaaaaatg
    13561 ttgttttgga taaaaatata gacggagaat ttgttttaga tactgaaatt acccccgccc
    13621 ttaaagagga gggcatggca agagagataa caagaatcat acaaggtttg agacaagagg
    13681 ctaatctgaa accaaaagat aggatagatg tttttataaa aactgaaagc caagttgtct
    13741 ccagcattgt agtcagatgg gataaagaga taaaagaagt aaccggagcg gatagggtaa
    13801 gactggaaaa atcagataaa tttaaagtag aaacagaaac gcacttagaa ggagaatctt
    13861 tatgggtggc gattaagaag gtctaggttg tagttataac cgtgtagttg gtagaatata
    13921 aaaatgacaa aacccatcgt tataagcggg atcaaacctt ccggagaact tcacatagga
    13981 aactatttgg gaatgctgcg gaatgcttta gctctacagg aatcaaataa atataattgt
    14041 ttttatttta tcgccgatta ccatgcgatg acccaaaaat acgaccctaa agatttgagg
    14101 gagcagacga ttagcgttac cgcagacctt ctagccacag gcatcaatcc taaaaaatca
    14161 gcgattttta tccaatcgca tgtgccggag catgcgaatc ttgcatggat ttttaataca
    14221 attacaaccg tatctcaatt agagcgaatg gtggaatata aagaaaaatt aggcgatgga
    14281 caagttccca ataccggact ctttgattat cctgtcttga tggcggctga catccttgtg
    14341 tataaatcca gaagagttcc ggttggagaa gatcaaaggc agcatttaga attagctagg
    14401 gatatagcaa ggacattcaa tggcaggttt ggcgaaacgt ttaaggagcc gaaagcggtg
    14461 ttaaccagga ttccacgcgt gatgagttta aataaccctt ctaaaaaaat gtcaaaatca
    14521 attcccagcg gctgtcttta tctgcaggat tcgccaaaag caataagaga aaaggttatg
    14581 tccgcagaga ccgattctat gcgagagata ggttatgatc cggcgacaag actcggggtt
    14641 tctaatctag tgcttatata ttcagaattt tcaggagata ctcctgatga agtggttaat
    14701 aaatttaccg gcaaaggata cagagagttt aaagaatctt tagcggattt aattattgag
    14761 aaattagcag attttcaaga aaagagaaaa aaaatcttga aagataaaag ggctataata
    14821 aaaattttgg agtctgggga aaagagggtt tctccaatcg cagagaagac aatggaagag
    14881 gttaggagga aaaccggcct gatataataa agccactagc gactagcgtt tagcgactcc
    14941 aatgaaatct tataaggaat taatagtttg gcagaagagc gttgaattag tagaggaaat
    15001 ttataaatta accccttttt tccctaaaga cgagatctat gggatttcca gccagatgaa
    15061 aagagctgtg gtttcaatac cgtctaatat agccgaaggt tttgtcagaa aacacaataa
    15121 agaattccaa caatttataa gaactgcttt tggctcagcg gcagagttgg aaactcaact
    15181 cataatagct aaaaagttga gattggctcc gagtaataat tttaataaaa tagaggctct
    15241 tttgaatgag atcctgaaaa tgctcaatag gcttatttct tcactttcca ttgatcacta
    15301 gtggctaatc actagtcgct aaatttctat tatgacaggt aaaaccattg gcctgcggaa
    15361 agttttatta taaagatatt ggcctatttg atcacggaat aaatttttca agtagtcggc
    15421 atccaacggt tgatgcttag gaatccttcc gataataccc cttatctttt ttcttacttc
    15481 ttcgagtaaa tattgattgt ctttcaaata aataaatccg cgcgatataa tgtccggatt
    15541 cttcaagatt ctgccggttt tcctgtcgat cgttgtgatg acgacgatca ttccctcctg
    15601 agcgagtatt cttctgtcgc gcaacaccac ttctcctacg tcgccgaccc caagaccgtc
    15661 caccataata taatgagccg gcacggtttc gtcagttata ataaaagagt cttttgtaag
    15721 actgcccact tgcccattat ccatcaggcg tatattttct ttaggcattc cggcgtcaat
    15781 tccattctgc ccgttggcgc ctctcataaa ataatacccg tgaacaggca caagaaactt
    15841 tggctttata agttgtatca cttttttaat gtcttctttg ggcgcgtgtc cgctggagtg
    15901 aatatctatc attttagacg tataaacaat tgctccctgg cgcgacaaat tgtctttaag
    15961 gttctgcaca cttctttcgt tgcccggaat gactgaagaa gaaaatataa cggtgtcgcc
    16021 ggcttttata tttatatatt tatgctcgcc gtttacgatt ttcatcaatc cggcggttga
    16081 ttctccttgc gcgccggttg taataaccaa tattttctca tctttgtgct tatgggcttc
    16141 ttctacggag ataatcgtac ccttcttaaa tttcatatat ccaagattct gggctatttg
    16201 tatgttgtcc ttcatagatc tgccgtttat cacgactttt ttatccagct tctcagctat
    16261 cttaataatc tcggctatac gcgtgagcat agatgaaaaa gttgaaagaa ttatccgccc
    16321 ctccgccttt ttaaataata tttcaaggtt tttctctact gttttttcag gcaaagattg
    16381 cccctcttcc tcggcattag tgctatcaac cataaatgta tgaatgccga gctcgccgat
    16441 tcttttaaat tcatcgaact tttcgggttc accggcttcg ttgtaatcaa ctctgaagtc
    16501 ggcaaagtga acgatattta aaaccggcgt ttttatgacg actccggtag tgtccggtat
    16561 ggtatggtcc acgccgaata tttctatctc aagatatttc ccgagtttaa cttttgtgcc
    16621 attctctaca acctcaacta taagctttgg cgcattaacg aattcttcct gccttctttc
    16681 aataatagct ttggttagtt ttgttgtgta tatagttggg ttgccaagtt tgccaataag
    16741 gtagggtatt gccgccacgt ggtcgaggtg cccatgtgta agaactatac ctttaatatt
    16801 ttgcttcttg gggattagat attctatgtt gggaatgata aaatctatgc ctggagtttc
    16861 ttcttccgga aattgtattc ccatatctat aatcacaatc tcgtctttat attcgacgaa
    16921 agccatattt cggccaactt cttccaagcc tccaaggggt actaaacgaa caacatcgtc
    16981 tgacgctgga tgtttttgtt tgggtttttt atgtggatat tttgactcct cagacattat
    17041 tttggaagtt ctatatcttc cacttgaaga tgcctgtgtt cttctgtgcg ttctagtttg
    17101 catattttta ttatttttat ttttattgtg ggcgagagag gacttgaacc tccacgagat
    17161 tgctcccact gactcctgaa gccagcgcgt ctgccatttc gccactcgcc c
//
LOCUS       DATDNZ010000078        11204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2087299, whole genome shotgun sequence.
ACCESSION   DATDNZ010000078 DATDNZ010000000
VERSION     DATDNZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087299"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VI432_03020"
     CDS             <1..513
                     /locus_tag="VI432_03020"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY4497093.1"
                     /translation="LLAMGLYFILDTIMIAILAAVAENGVIGVDNDLPWHLPEDLKKF
                     KELTTGKVVVMGRKTYESIIKRLGKPLPNRKNVVITRQTGYKALPDVDIFYNLEDALN
                     ANKSNDIFIIGGGEIFKQSIDKANMMYITHVHETYEGDSRFPKIDMKNWKLIEEEKRN
                     GFSFAIYKRR"
     gene            complement(603..899)
                     /locus_tag="VI432_03025"
     CDS             complement(603..899)
                     /locus_tag="VI432_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497094.1"
                     /translation="MAKEEWMPIKEKELFALRNVFEELRGARPYFDRLPPVVQDRLNR
                     VLEGVLDPKRRERIRLGIRAECDCPEPGSPDGSTFGRCSFCGGILKLKMVQARL"
     gene            complement(967..3966)
                     /locus_tag="VI432_03030"
     CDS             complement(967..3966)
                     /locus_tag="VI432_03030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY4497095.1"
                     /translation="MTSKKFQELYNKLNSAQRQAVDEIEGSVMVVAGPGTGKTQILTL
                     RIANILKKTDIEPQNILALTFTESGVVSMRRRLSEIISTSAYSVNINTFHGFCNDLIK
                     NYPEKFPRIIGSENITEVEQLKIIEEIVESIQLKILRPFGSPFLYVRDIVSAINDVKR
                     EGVAPEKFNKIIDKEYKLVEGASDLYHDKGAYKGKMKGMHVKTLENIAKNKEFAKVYA
                     KYEKRLEKENLYDYSDMLVEAMRAMESDKDLLLTLQEEYQYILVDEHQDTNNVQNRIM
                     ELLTNFHENPNIFVVGDEKQAIFRFQGASLENFYYFRNIYPKAKIIVLEENYRSTQGI
                     LDSAHSVLAGEKELKSNYREKSKIRLYPFQRREASNFFIAKDINRLISEGIDPAEIAI
                     IYRENKDAHAIADALEREGVPYTIESEENILLDPEVRKILLLLRAVAEFGSPEAFIEA
                     SHIDFIDIEPLDVYKLVRYSADNKIDVLDLTKNITTLEKIGLGNPDKINDFYNNLSRW
                     SRISKNRGLTEFFEIIINESGALQHILAESDPFGKMDKLNAVFDAVKDIAERNKKARL
                     EELMEYFDILERHNRSIGRSIIHRTGGRVRLMTAHRSKGQEFEYVFLINCVYGHWGDK
                     RRPERIKLPDAVYSLTGKAFKDSVEDGDERRLFYVALTRAKKMVSMIYAKDESSKGDA
                     LPSRFIEDIDDDLIESVGTERYEKEFDSKIGAQFTPRRAVGPGLKDKEFVRELFLRKG
                     FSVTELNNYLRCPWRYFYTNLIRVPRAPNKNQMYGIAIHAALNDLFSGGAEPVRQAQG
                     KPTKTRLLKNFEEYLNKQPLSEVDFEEMFQKGKSALSGYFDKYHKDWNAVLKTEFNVR
                     GVELTPEIRLTGKIDRIDVLDDRGNVKVIDYKTSQPKSRAEIEGKTKAIGAGDAKRQL
                     IFYKLLLDNMDNSKLKMVSGEIDFVEPDAKGRYKKEEFVINDEEIKNLEELVKKTANE
                     ILEMSFWDRKCDDNKCEFCALRGILKS"
     gene            4059..4304
                     /locus_tag="VI432_03035"
     CDS             4059..4304
                     /locus_tag="VI432_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497096.1"
                     /translation="MESSTKVTAPKDQAKKLGAVDLVIARAEALKGLALAIKWSLQAD
                     DPDVDQDEVARIIMINLDGSAKDSLTESIDALGEALG"
     gene            complement(4686..5717)
                     /gene="ychF"
                     /locus_tag="VI432_03040"
     CDS             complement(4686..5717)
                     /gene="ychF"
                     /locus_tag="VI432_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY4497097.1"
                     /translation="MKLSIGIVGLPNVGKSTLFKALTNIEINIANYPFATIDPNVGVV
                     AVPDERLQKLAEMSKSEKIIPAVVEFYDIAGLVKGANKGEGLGNQFLTHIRDVQAIVQ
                     VVRVFGGSEIIHVEQIVDPIRDIEIINTELILKDLDTVEKRLQKLEGEARSGDKEKKK
                     DLEIVNQIKKILEEGKLLFTLSAEVLESLIMREMNLLTGKKQIYLLNGDEGDVSDDLK
                     AKIKELGADYIVADLSTQQHSNILQNVGMLALIKKAYEVLGLISFFTTGEKETRAWTI
                     KRGSLAPEAAGAIHSDFEKKFIRAEVIGWDKLLEAGGWAKAKQKGWLRLEGKDYVFQD
                     GDVTEIRHG"
     gene            complement(5955..7736)
                     /locus_tag="VI432_03045"
     CDS             complement(5955..7736)
                     /locus_tag="VI432_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4497098.1"
                     /translation="MAVEQRNKNGAWKILFRHIKEHKASIILVAILGIFSALANGTGP
                     FIMGSFLDSLIDTGSMVNILGITLATWSALLVLWAIVQSSASIIDWIIDRKGRGLRLA
                     MQFGYQSQAFIHLLSLPLSFHKNEKIGEMTDLIGRVGWQISSITDNILIRLTPPVLSV
                     IVGIIVTLLINPIFTLILIIGVILYVLTLVKLLAPATDFIKETHLAWSQSFADAYQAL
                     WNVQSVKHMTAEKHESQKIKNNFMNKIVGSRNRIELLWSNMDFYQRIIVLITQIVIFL
                     TSIYLIQHGDLTIGELVALNAYAGLLFGPFIILGHSWQILQDGLAAIVQTEKKIFDVP
                     IENYTPPNAYTPEKINGEVVFDKVSFSYNDSTEVLKKLNFKVGKGEKVALVGKSGVGK
                     STAIDLISGYYFPTEGKVLVDGHDTREFDLHALRRGIAIVPQEPILFNDTVMMNIRYG
                     KLQATDDEVIEAAKKAYAHDFIKEFPKKYDQLVGERGIKLSVGQKQRIAIARAILRNP
                     SILILDEPTSALDAETESFLAKTFEELMKGRTTFIIAHRLSTVRKADKIIVMDKGRVV
                     ETGTHDELIKKKSGLYRKFYDLQIDLR"
     gene            complement(7847..9085)
                     /locus_tag="VI432_03050"
     CDS             complement(7847..9085)
                     /locus_tag="VI432_03050"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY4497099.1"
                     /translation="MNKEDLYKKLPESPGVYLMKGVKGKLLYVGKAVNLRRRVSSYFI
                     KAHDSRIEKLVSEIKKVDYKETDSALEALILEAELIKKYQPLYNIREKDDKSFLYVEI
                     TREKFPRVLLVRGKNISPLASSGRNASYFGPFTSASQIREALRIIRKIFPFSIHKLDV
                     IGQVSGVERKRPCFDYQIKLCPGTCIGVINRADYMKNIRNIKLLFQGKKERILKNLDK
                     EMISASKSLDFERAAKLRGKIFALKHIQDIALISDSTGQMSGVNGQMLSRIEGYDISN
                     ISGIHAVGSMVVFRGDRPSRSEYKKFKISTIDSPNDVGMIKEVLKRRMSHREWGTPDA
                     ILIDGGKGQVNGAKSVLNEFGIEIPVVGIAKGAKRKKNEFVGDIPKNIDKNILIRVRD
                     EAHRFAVSYHKKLRGRRFMN"
     gene            complement(9142..9462)
                     /locus_tag="VI432_03055"
     CDS             complement(9142..9462)
                     /locus_tag="VI432_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497100.1"
                     /translation="MDRDVFNRWVHIVEKSTTHDLSVDETLFLKDHISISELIKQVLA
                     LAFNVRVKIDLQGKMLRIDNEKYYLCEHIIMAVHAAIRFGLIDVGHRVFQNMKFRIPE
                     GYAS"
     gene            complement(9592..10059)
                     /locus_tag="VI432_03060"
     CDS             complement(9592..10059)
                     /locus_tag="VI432_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497101.1"
                     /translation="MERGARIEVDRRILDRAQYPFVIEERSLASDMHRITLVDIGIEF
                     DPSVDPIERLLKIMADLSKQQGMRKATEGDVLALCAHNPDLARRFCVVASGAILGAKS
                     SGSVGQIYPLDVLCLTDVGEGQFVAGLRQYAKILTRLDQTNLLMPIRVAFACE"
     gene            10167..10946
                     /locus_tag="VI432_03065"
     CDS             10167..10946
                     /locus_tag="VI432_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497102.1"
                     /translation="MRLEQPEFIPPTQEQKSTPIMDFLVKEAEYLRENGFLVMDDCRL
                     ETQVEPGERAQKRQEEWGENRNERDGERLEQLTTVVLNEYFSYSNQKSGDDLFVIRSN
                     EHDDTWNATDNFLIDKKTGKILAAFDEVVDDPKGRKFEDKIDKSRIINEKGGARISFT
                     PNKRDDKPGEATPSENIPHLVIRLSKAQLDNAMSMIAKIGTSLTKKTQFMTGVFDIFA
                     KQIESQIADLDKSLGLDRNVANNLYFLEQTLKKAQSSAGKK"
BASE COUNT         3367 a         2542 c         1854 g         3441 t
ORIGIN      
        1 ttattagcga tgggtttata ctttattcta gatactatta tgatagcaat attagccgct
       61 gttgcggaaa acggagtaat tggagtagat aatgacttgc catggcacct gccggaagat
      121 cttaaaaaat tcaaagagct gacgaccgga aaagtcgttg taatgggtag aaaaacctat
      181 gaatctatta taaaacgcct ggggaaacca ctgcctaacc gcaaaaatgt tgtgataaca
      241 agacagacag gatataaagc tctgccggat gttgatattt tttataattt agaagatgcg
      301 ctaaatgcca acaaaagcaa cgatattttc ataataggcg gcggtgagat attcaaacaa
      361 tcaatcgata aagcgaatat gatgtatatt actcatgtcc atgaaactta tgagggcgac
      421 agccgctttc caaaaattga tatgaagaac tggaaactaa ttgaggaaga aaaacgaaat
      481 ggatttagtt ttgcaattta taaacgaagg tagacagccg caacattcca acattctata
      541 gaatgttgga atgttgctaa aaaaacgagg cgacccgatg tggtcgccca aagagttcag
      601 ctctaaagtc ttgcctgaac cattttcaat ttaagtattc ctccgcaaaa agaacagcgg
      661 ccgaaagtgc ttccgtcagg agagcctggc tcaggacagt cgcactcagc gcgaataccc
      721 agcctgatcc gttcccttct tttaggatca agcactcctt ctaaaaccct gttcagccta
      781 tcttgcacaa ccggcgggag acggtcgaag tacggtcttg ctccacgcaa ttcttcaaaa
      841 acgttacgaa gcgcaaaaag ctccttttct tttatcggca tccattcttc ttttgccata
      901 tatatcccat cagttcttat cagagtgctg atttaaggta actacaataa tatactattg
      961 tcaaagtcaa ctcttcaaaa tgccacgcag agcacagaat tcacacttat tgtcgtcgca
     1021 ttttctgtcc cagaaagaca tctctaaaat ctcgttagcg gtttttttaa ctaattcctc
     1081 aagatttttt atttcttcat cgttaattac gaactcttct tttttatatc tgcccttcgc
     1141 atcgggttcc acaaaatcta tttctccact caccattttt aatttcgagt tatccatatt
     1201 gtctaaaagt aatttataaa aaattagctg tctcttagca tctccagctc caattgcctt
     1261 ggttttccct tcgatctccg cgcgagactt cggttgagag gttttataat caataacttt
     1321 aacatttccc ctatcatcca gaacgtcgat cctgtctatc ttccccgtta atcttatctc
     1381 cggtgtaagc tctactcctc gaacattaaa ttcagttttg agaactgcgt tccaatcttt
     1441 atgatattta tcaaaatatc cgctaagcgc gctctttcct ttttgaaaca tttcctcaaa
     1501 atccacctca ctaagcggtt gtttattaag atactcttca aaattcttca gtaaacgggt
     1561 ctttgtgggc ttgccctgag cttgccgaac gggttctgcc ccgccggaga ataaatcatt
     1621 caatgcggca tgaatcgcta ttccatacat ctgattttta ttgggcgctc taggtactcg
     1681 aattaaattg gtataaaaat atctccacgg acatcttaaa taattattaa gttctgtcac
     1741 agagaatcct ttacgcagaa atagttcgcg tacaaactcc ttatccttta accccgggcc
     1801 cactgctcgt ctaggggtaa attgagcacc tatctttgaa tcaaattctt tctcatatct
     1861 ctcggtgcct accgactcaa tcaaatcatc gtctatatct tcaatgaatc ttgagggcag
     1921 ggcgtcccct tttgaagatt cgtcttttgc ataaatcata gataccatct ttttagcgcg
     1981 ggttaacgcc acatagaata atcttctttc atctccatcc tccaccgaat ccttgaatgc
     2041 cttgccggtc aaagaataaa ccgcgtcagg caacttaatc ctctccggtc ttctcttgtc
     2101 tccccaatga ccataaacgc aatttataag aaatacatat tcaaactcct gccccttaga
     2161 tcgatgcgca gtcatcaagc gcacccttcc gccggttcta tgaattatgc ttcttccgat
     2221 agaacgatta tgcctttcca atatatcaaa atattccatt aattcttcca atcgcgcctt
     2281 cttatttctc tcggcaatat ctttaacggc atcaaaaacg gcatttaatt tatccatttt
     2341 tccgaaaggg tccgattcag caagaatatg ctgcaatgct ccggactcat tgattattat
     2401 ttcaaaaaat tctgtgagtc ccctattttt cgaaatccta gaccatcgac ttaaattatt
     2461 ataaaaatca ttaattttat caggattgcc caatccaatc ttctccaacg ttgtaatatt
     2521 ttttgttagg tcgagaacat caatcttatt gtctgcggaa tatctaacaa gcttataaac
     2581 atctaatggc tctatgtcga taaaatctat atggcttgct tcaataaatg cctctggaga
     2641 cccgaattct gcaaccgccc tcagcaagag caatatcttc ctcacctcgg ggtcaagtaa
     2701 aatattttct tctgattcaa tcgtatatgg gacgccttct ctctcaaggg catcagctat
     2761 tgcatgcgca tctttattct cacgataaat aatcgcaatc tctgcaggat ctatcccctc
     2821 actaattaat ctattaatat cttttgcaat gaaaaaattg ctagcctctc gacgctgaaa
     2881 tggatataat cttatttttg acttctctct ataattagat tttaattctt tctctccggc
     2941 gagcacagaa tgcgctgaat ccaatatccc ctgtgtcgaa cggtaatttt cttctaggac
     3001 gataatcttt gctttgggat atatatttct gaaataataa aaattttcta atgacgctcc
     3061 ctgaaaacgg aatattgcct gcttttcatc tccgaccaca aaaatatttg gattttcatg
     3121 aaaatttgtt agaagttcca taatcctgtt ttggacattg ttcgtatcct gatgctcatc
     3181 aactaaaata tattgatact cttcctgcag tgttaataat aaatccttat cagactccat
     3241 tgctcgcatc gcttcaacca gcatatcgct gtaatcatat agattctctt tttcaaggcg
     3301 tttttcatat tttgcataaa cttttgcgaa ttccttattc tttgcgatat tctcaagcgt
     3361 cttaacatgc attcccttca ttttgccctt atatgcgccc ttatcatggt agagatcaga
     3421 cgcgccttca accaatttat attctttatc aataatctta ttaaattttt cgggagcaac
     3481 tccttcacgc ttaacatcgt ttatagcgct cacaatatcg cgcacataaa gaaacgggct
     3541 gccgaacgga cgcaggattt taagttggat ggactcaact atctcctcga taatttttaa
     3601 ctgctcgact tcggtaatat tctccgaacc tataatacgc gggaatttct ctggataatt
     3661 tttgattaaa tcattgcaga agccgtgaaa ggtattgata ttcactgaat atgcgctggt
     3721 gctaattatt tcggaaagtc gacgcctcat cgaaaccact cccgactcgg taaacgtaag
     3781 agcgagtatg ttctgcggct caatgtctgt tttcttaaga atgtttgcga ttcggagtgt
     3841 caaaatctgt gttttccctg ttcctgggcc ggcaacgacc ataactgatc cttcaatctc
     3901 atccacagcc tgacgctgtg ctgaatttaa cttgttatat aattcttgga atttcttgga
     3961 tgtcatttat tttattctat cagaaaacca tatttactaa ttgacagtag aacgatatta
     4021 agattatatt ggcaaaagta cacaaaggag ggaaataaat ggaatcatca acaaaagtca
     4081 ccgcaccgaa agatcaagca aaaaagctcg gagccgtcga tcttgtcatc gctcgcgctg
     4141 aagcccttaa gggcttggct cttgctataa aatggtcact gcaagcagat gatcctgacg
     4201 tagatcaaga tgaagtcgca agaataatca tgattaatct tgatggatca gcgaaagatt
     4261 ctctcaccga gtcgatcgat gccctcggag aagcgctagg ataaacgtcc cctataattc
     4321 gcgaaccaca catgtgtggt tcttttttca tccccctttt atccccatgg ggacaaaagg
     4381 gggatgaaca tctccgtctt gcccttcaaa aattcaggac tacgactata tattttatta
     4441 attaatgact gattaaatca tcaaacaata actatccatg acgaatctcc ataacatccc
     4501 catcttgccc attcgataga ctcaggactg tgactaccgc atcattaaaa ttagaacaac
     4561 caccttatta aattttatct aaccgtgacg tatttctgtc acatccccgt cttgcccttc
     4621 gaccaccctc aggactgcga ctgcccataa cacgtttcaa tcactaacga actcgtcaaa
     4681 aaaaattaac cgtggcggat ctccgtaaca tcgccgtctt gaaatacata atctttacct
     4741 tcaagacgaa gccagccctt ttgcttagcc ttagcccatc cacctgcctc aagcaacttg
     4801 tcccatccta taacttcagc tctaataaat ttcttttcga aatcagaatg tattgctcct
     4861 gccgcctccg gcgccaaaga ccctctctta atagtccatg cacgagtctc tttctcgcct
     4921 gttgtaaaaa agctaataag ccccaagacc tcatacgcct ttttaatcaa ggctaacatt
     4981 ccaacattct gcagaatgtt ggaatgttgt tgagtggata aatcggcaac tatataatct
     5041 gcgcctaatt ctttaatttt ggctttcaaa tcatcgctta catcaccctc atcaccattc
     5101 aatagatata tctgcttctt tcctgtcaat aaattcatct cacgcataat taagctctct
     5161 aacacctctg ccgacaaggt aaaaagcagc tttccctctt ccaatatttt ttttatctga
     5221 ttaactattt ctaaatcttt tttcttttct ttatccccgc ttctagcctc accttcgagc
     5281 ttctgcagtc ttttctcaac cgtatccaaa tcttttaata taagttcggt gttgatgatt
     5341 tctatgtctc ttatcggatc cacaatttgc tcaacgtgta ttatctcgct accgccgaaa
     5401 actcgcacaa cttgcactat cgcctgcacg tcgcggatat gagttaaaaa ttgattgcct
     5461 aatccctcac ctttattcgc gcccttaacg agtccggcga tgtcataaaa ctccacgacg
     5521 gcaggaatta ttttttccga cttactcatc tcggctaatt tctgtaatct ttcatctgga
     5581 acagcaacca cgccaacatt cggatcaata gtcgcaaacg gataattggc gatatttatt
     5641 tcaatatttg taagcgcctt aaaaagcgtt gatttaccta cattagggag gcctactatg
     5701 ccaatactta atttcataaa tatagataat cactataaat ataatccatt caattgtaaa
     5761 tcataaaatg ctataatact tacaaatgaa gccaataatt cctcaagttt gttttagttg
     5821 cgagaagccg cttatcaaga aagtagatat aaataaacgc ggcattaaat cacaaacttg
     5881 cacttggtgc gggaaaacac aagctccacg ccaatcacaa agaccgttta attccagaaa
     5941 gaaataatct ccacctatct taagtctatc tgcagatcgt aaaacttacg atacaagccg
     6001 cttttctttt tgataagctc atcgtgagta cctgtttcaa ctactcttcc cttatccatc
     6061 acgattattt tatccgcctt tcttactgtg cttaaacggt gggcaatgat aaaagtcgtg
     6121 cgtcccttca ttaattcttc aaatgtcttt gcaagaaaac tttcggtttc cgcatcaagc
     6181 gcgcttgtag gctcgtcgag aatcaaaatt gatggattgc gcaatatagc ccgagcaatc
     6241 gcgatgcgtt gtttctgccc taccgacaat ttaattccac gctcccccac aagctgatcg
     6301 tacttcttcg gaaattcttt aataaaatcg tgtgcgtaag cttttttagc cgcctcaatg
     6361 acttcgtcat ctgttgcctg taatttaccg tatctaatat tcatcatcac tgtatcgtta
     6421 aaaaggatcg gctcttgcgg aacaatagca atgcccctgc gcagcgcatg cagatcaaat
     6481 tctcttgtat catgtccatc aactaaaacc tttccttccg taggaaaata atatccggaa
     6541 atcaaatcta tagcagtgct tttcccaacg ccagacttac caaccaaagc gactttttca
     6601 cccttaccaa ctttaaagtt caatttttta agaacctctg tcgagtcatt ataagaaaaa
     6661 gagactttat caaaaacaac ctctccatta atcttttcag gagtatatgc attaggcggg
     6721 gtataatttt ctatcggaac gtcaaaaatt ttcttttctg tctgaactat agcagctaga
     6781 ccatcttgta atatctgcca gctatgacca agtataataa aaggtccaaa caacaatccc
     6841 gcataagcgt ttaaagccac gagttcaccg attgttagat cgccatgctg gataagataa
     6901 attgatgtca aaaatataac gatctgagta attaaaacaa taattctctg ataaaaatcc
     6961 atattgctcc acaaaagctc aatcctattc ctagacccaa cgattttatt cataaaattg
     7021 tttttaatct tttgactctc gtgcttttca gccgtcatat gcttaaccga ctgaacattc
     7081 catagggctt gataagcgtc agcgaaagat tgactccatg ctaaatgagt ctctttgata
     7141 aaatctgtgg caggtgctaa taattttacc aaagtcagta cgtataaaat tactccaatg
     7201 attaagatca aagtaaatat ggggtttatt aaaagagtaa cgataattcc tacgatgaca
     7261 ctcaagaccg gcggagtaag cctgattaat atattgtctg ttattgaaga tatttgccaa
     7321 cccaccctgc ctataagatc ggtcatttcc cctatctttt cattcttatg gaatgataga
     7381 ggcagagaca ataaatgaat aaaagcctga ctctgataac caaactgcat cgccaatctt
     7441 aagcctctgc ctttcctgtc aataatccaa tcgataatac ttgcgcttga ctgaactatc
     7501 gcccacaaga ctaacagagc cgaccaagta gcaagggtta ttcctaagat atttaccatg
     7561 ctcccagtat ctatcaagga atccaaaaaa ctgcccataa taaaaggacc tgttccatta
     7621 gctaaagctg agaaaatacc taaaatagcg actaaaataa tagaagcttt gtgttcttta
     7681 atatgcctga ataaaatttt ccaggcacca ttcttgtttc tttgctcaac cgccatgagc
     7741 ttaatagtag cctctattct ttttttgaca atattgaata tctatatacc tatgctataa
     7801 ttcattacat aaaaaagact gcgtgaggcg gtctttttta atttgtctaa ttcataaatc
     7861 ttctccctct caatttttta tgataactca ccgcaaatct atgggcttca tcacgcacgc
     7921 gaattaaaat atttttatct atatttttag gaatatcgcc cacgaattca ttcttttttc
     7981 gttttgcgcc tttggcaatg cctacaactg gaatctctat tccgaattca tttagaacag
     8041 acttcgcgcc attcacttgc cccttgcctc catctatcag aatggcatcc ggggttcccc
     8101 attcacgatg agacattctc ctctttaaga cctctttaat cattcctaca tcattgggac
     8161 tatcaatcgt gctgatttta aatttcttat attcacttct actcggccta tctcctctaa
     8221 aaacgaccat tgacccaacc gcgtgaattc cagagatatt agatatgtca taaccttcaa
     8281 tgcgagacaa catttgacca ttaacacctg acatttgacc ggtagagtcg gatataagag
     8341 ctatatcttg aatgtgtttt aatgcgaaaa tctttccgcg aagctttgcg gcacgctcga
     8401 aatctagcga cttgctcgcg gagatcattt ctttgtccaa atttttaagt atcctttcct
     8461 ttttgccctg aaacaacaat ttaatatttc tgatattttt catataatcc gcgcggttaa
     8521 taacgcctat gcatgttccc ggacaaagtt ttatttgata atcaaaacac gggcgcttac
     8581 gctctacacc tgacacttga cctataacat ctaacttatg gatggaaaac ggaaatattt
     8641 tacggattat tcgcagggct tcgcgtattt gagaagctga agtaaaagga ccgaaatacg
     8701 aagcatttcg tcctgagctt gccaaaggac tgatattttt acctcttacg aggagtactc
     8761 gcggaaattt ttctctagta atttcaacat ataaaaagct tttatcgtct ttttcacgga
     8821 tattatatag cggttgatat ttctttatta gttccgcctc taaaatcaac gcctcaagag
     8881 cagagtcggt ttctttataa tcaaccttct taatttctga aaccagcttc tcaatcctag
     8941 aatcatgggc ttttataaaa taactggaaa cgcggcgcct taaattaaca gctttgccaa
     9001 cgtataacaa ttttccttta actcccttca ttaaataaac acctggagat tcgggcagtt
     9061 ttttatataa atcttcttta ttcattacat aagttttaac agatatatgc taaaaagaaa
     9121 aagccacata tatataagtg gctacgatgc gtatccctcc ggaattctga atttcatatt
     9181 ttgaaatacc ctgtgaccca catcaatcaa accaaatctg atagcggcat gaacggccat
     9241 tataatgtgt tcacatagat agtatttttc attatcaatg cggagcatct tgccttgcag
     9301 atcaattttt actcttacat taaaggcaag agctagaact tgttttatca attcacttat
     9361 tgaaatatgg tcttttaaaa acagggtttc gtctacagat aaatcgtgag ttgtgctttt
     9421 ttctactata tgaacccatc ggttgaaaac atctcggtcc atcccatcat gttccagcat
     9481 tctattcgct ccttctaaag tgctgttgca taaaatagcc tatattaaca aatcagtcaa
     9541 aaaaaacgag acctcgttag agatcccgaa caaagaactg tattgacgct cttattcgca
     9601 cgcaaatgcg acacgaatag gcatcaacag attagtctgg tccagcctag tgagaatttt
     9661 tgcgtactgg cgcaaaccag ccacaaactg tccctcacct acatccgtaa gacacaacac
     9721 atccaatgga taaatttgcc ctacacttcc agaactctta gcgcccaata tggcgccaga
     9781 agcaacaacg cagaaacgtc ttgcaaggtc tggattatga gcgcatagag caagcacatc
     9841 tccttctgtc gccttacgca tgccttgttg cttgctcaaa tcggccatta tctttaacaa
     9901 tctttcaatc ggatccacag aaggatcaaa ttcgattccg atatccacca gcgtaatcct
     9961 atgcatatcc gacgcaagag acctttcctc aataacaaat gggtattggg ctcggtctaa
    10021 tatgcgccga tcgacttcga ttctcgcgcc tctttccact aggaatgttt tgtttctatc
    10081 cagaaaacca ccctccgaat tttcagagaa ctcacagatg ataatacaat cttgacaaat
    10141 tgtcaaatta gcatattatt aataatatga gacttgaaca gccagaattt atccctccga
    10201 cacaggaaca aaaatctact ccgataatgg actttctcgt aaaagaagct gaatatctgc
    10261 gtgaaaatgg attcctcgtt atggacgatt gcaggctaga gacacaggtg gagccgggcg
    10321 aaagagctca aaaaagacaa gaagaatggg gtgaaaacag aaacgagaga gatggagaaa
    10381 gattagaaca attaacaacc gttgtactta atgaatattt cagctatagc aatcaaaaaa
    10441 gcggagatga tttattcgta atcagatcta acgagcatga cgacacgtgg aatgcaacag
    10501 ataatttttt aattgataaa aaaactggga aaatcttagc ggcatttgat gaggtggttg
    10561 acgatcccaa aggaagaaaa tttgaagata aaatagataa aagcagaatt ataaatgaga
    10621 agggcggagc tagaatcagt tttactccaa ataaaagaga tgataagcca ggagaagcca
    10681 ctccatccga aaatattccc catcttgtaa taaggttgtc taaagctcaa ctagataacg
    10741 ctatgtctat gatagctaaa ataggaacat ctcttacaaa aaaaacgcaa tttatgactg
    10801 gggtttttga tatattcgct aaacagattg aatctcaaat cgcagattta gataaatcat
    10861 tagggttaga tcgcaacgta gctaataatc tttattttct tgagcagact cttaagaagg
    10921 ctcagtcttc tgcaggcaaa aaataaagat cccatgcggg atcttgattc gggtttagac
    10981 ttgaagaaga tgctagtcgt tcaaactaca gagtagtttg gtccgaacta gctgcccgtc
    11041 gaaggggtcg ccggcgaagg agaactttct ccagcacgct cggtgtactc ccagtcgtcg
    11101 gtcacgatac gcgtccagtt gccattcatc ggttgcgttc ccattttctt catctcctct
    11161 gctataaaaa gcagagtttg ttgtactaaa aaaagattag caca
//
LOCUS       DATDNZ010000079         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_2864863, whole genome shotgun sequence.
ACCESSION   DATDNZ010000079 DATDNZ010000000
VERSION     DATDNZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864863"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VI432_03070"
     CDS             complement(<1..764)
                     /locus_tag="VI432_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4497103.1"
                     /translation="MNRRNAQIFFFLSLLIISIVISFFIVRPFISTLILAIVAAVVFY
                     PIHKMFYKFFKAKWLAATLTILIFFSTILAPMSFFGVRVVEEAQTAYRYFTETIDGIV
                     LISNVESFINKNIFERFLNIEQETEGSFFLDFNSYVQDVFNWLVRNFGAVASGLARVG
                     INLMLFIFAMFFLLRDGEGIKKFIVGLSPLDDKYDEEIFKRLSKSINSVIRGSLMIAV
                     IQGILAGVGFTLFGIPSPILWAGAVMVAALVPALGTA"
     gene            complement(882..965)
                     /locus_tag="VI432_03075"
     tRNA            complement(882..965)
                     /locus_tag="VI432_03075"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(929..931),aa:Tyr,seq:gta)
     gene            complement(1050..1337)
                     /locus_tag="VI432_03080"
     CDS             complement(1050..1337)
                     /locus_tag="VI432_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497104.1"
                     /translation="MYNLILQIIFMGSLGVIVYLFAIAVPRMQDSSDEGRSGLISRFF
                     SKLPLHRVDTALKSYKDKTLRRLKVLILRADNAVSKRINSQDKDKFIKPPQ"
     gene            complement(1462..1908)
                     /gene="tsf"
                     /locus_tag="VI432_03085"
     CDS             complement(1462..1908)
                     /gene="tsf"
                     /locus_tag="VI432_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY4497105.1"
                     /translation="MIKSEDVQRLREATNAGVMDCKRALEDAVGDFEKAVSLIKERGL
                     VKAEKKSSREAKSGIVESYVHNNRVGVLLELNCETDFVAREESFRELAHNIVMQIAAM
                     NPQTNEELLAQSFIKDDKMTIEDLIKSAIAKIGENIQVGKFIRYEI"
     gene            complement(1912..2745)
                     /gene="rpsB"
                     /locus_tag="VI432_03090"
     CDS             complement(1912..2745)
                     /gene="rpsB"
                     /locus_tag="VI432_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY4497106.1"
                     /translation="MEDIKTIIDSELEDSKSQEPEQVLTPEEEVMLKDMMANHVFYGY
                     SKSRTNPKMKPNIAMTKSGVEVIDAVKTMFFLNKAVDAIKQLVHGGDLVLLVGTSTAA
                     KSTVKEIATRLQMPYVDERWLGGTLTNFKTISDRINYFKKLEQEKALGKLAQKYTKKE
                     QLGIDVELEKFDKFFRGIENLNKLPKLVVIADTKYNEIAAKEAKKMKIKTMGIINTNS
                     DPDIVDYPIPANDRNSKSIAFIMSYLEKFIAEAKNNFPKDKVKEGDKLEGKELNKQLK
                     S"
     gene            complement(2818..>3002)
                     /locus_tag="VI432_03095"
     CDS             complement(2818..>3002)
                     /locus_tag="VI432_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY4497107.1"
                     /translation="ISGVLGKGESLEENRLGVGVPSYTRPETIVYKGKKYRVPLVLLS
                     GHHKDIEEWRMKNRRK"
BASE COUNT          932 a          653 c          464 g          953 t
ORIGIN      
        1 gcggtaccca acgctggcac aagagccgca accataaccg ctccagccca taaaatcggg
       61 cttggaattc caaataaagt aaagccaacg ccagctaaaa tcccctgaat aaccgcaatc
      121 attaaagacc ctctaatcac agaattaata gatttgctta agcgtttaaa tatctcttcg
      181 tcgtatttat cgtcaagcgg gcttaatccg acaataaatt tttttatacc ttcgccgtct
      241 ctcaacaaga agaacatggc aaagatgaaa agcatcagat ttataccaac cctagcaagg
      301 ccggaggcca ccgctccaaa atttctaaca agccaattaa aaacatcttg aacataactg
      361 ttaaagtcaa ggaaaaaaga tccttcggtt tcttgttcta tgttcaaaaa tctctcaaaa
      421 atatttttat taataaaact ctcaacgtta gaaatcaaaa ctatgccgtc aatagtctcc
      481 gtaaaatatc tataagccgt ctgagcttcc tcgacaaccc ttactccgaa aaaagacata
      541 ggcgctagaa tagtagagaa aaatattaat atcgttaaag tggcggccag ccacttagcc
      601 ttaaaaaact tataaaacat cttatgaata ggatagaaaa caaccgcggc gacaattgcc
      661 aaaattaatg ttgaaataaa aggtctaacg ataaaaaagc ttattactat ggagatgata
      721 agcagactca agaaaaagaa aatttgcgcg ttacgcctat tcataagtta taaatatatt
      781 gtatcaaaaa ttagtgattg tgtttatatt agacttgtat gggtgcgcgc catgtagata
      841 atcaaataaa atcttgtatt caagtctatt tataaaatag gtgcgccggg gaggattcga
      901 acctccgtag gcataaagcc gcgtgattta cagtcacgtg cgattgacca ctccgccacc
      961 gacgcatttt aagaaaccat agtctcgact taagtcggac catcgacacg cgcattaaag
     1021 tctaaaagaa ttctattctc tattcaagcc tactgcggag gcttgataaa tttatcttta
     1081 tcctgcgaat taattctctt actaaccgcg ttgtcggccc ttaaaatcaa taccttcaat
     1141 cttcttaaag tcttatcttt gtaagattta agagctgtgt ctaccctgtg cagtggaagt
     1201 ttagaaaaaa atcttgatat taatcccgaa cggccttcat cggatgaatc ctgcattctg
     1261 ggcacggcta tggcaaaaag atagacaata accccaagag accccataaa tataatttgt
     1321 aagattaaat tatacattat cgcgatgcga aattacgaat acatgcgaat ctacaaatag
     1381 atttagcgta tggattcgga tcattgttat ctttgtattc atcttcgtag attcgcataa
     1441 atttgtggtt tggaatcggt tttaaatctc gtatctgata aacttaccaa cctgaatatt
     1501 ctccccaatc ttagcaatag cgcttttgat caagtcttca atcgtcattt tatcatcctt
     1561 gataaatgat tgcgctagta attcctcatt ggtttgcgga ttcatagctg caatctgcat
     1621 aactatattg tgagcaagtt ccctaaaact ctcctccctc gctacaaaat cggtttcgca
     1681 attaagctct aagagcactc ccactctatt gttatggaca tatgattcta caatgccaga
     1741 tttagcttca cgagaagatt ttttctctgc tttaaccaaa cccctttcct tgattaaaga
     1801 aactgctttt tcaaaatcgc ctacagcatc ctcaagagct cttttacagt ccatcacgcc
     1861 tgcatttgtg gcttctctca gcctttgaac gtcttctgac ttaatcattt attaactttt
     1921 caattgttta tttaattctt tgccctccaa tttatcacct tccttaactt tatctttagg
     1981 aaaattattc ttggcctcag cgataaactt ttccaaataa gacattatga aagctatgct
     2041 ttttgagttt ctatcattgg cgggaatcgg ataatcaaca atatcaggat cagaattagt
     2101 gttaataata cccattgtct taattttcat cttcttagcc tctttagcgg caatctcatt
     2161 atatttggtg tcagctatta caaccaattt cggcagttta ttcaaatttt ctatgcctcg
     2221 gaaaaattta tcaaattttt caagctcaac gtcaatccct aattgttctt tcttggtata
     2281 tttctgggct aatttgccta gcgctttttc ctgttcaagc tttttaaaat aatttatcct
     2341 atcggagata gttttaaaat tagttaatgt tccgccaagc catctctcat ccacataagg
     2401 catttgaagc cttgttgcaa tttctttaac ggtgcttttc gcagccgtag atgtccccac
     2461 gagcaaaacc aaatctcctc catgaaccaa ttgttttatc gcatcaacgg ctttattcaa
     2521 aaaaaacata gttttcaccg catcaataac ctcaaccccg gacttagtca ttgcaatatt
     2581 aggtttcatc ttaggattag tcctagattt tgaataacca taaaaaacat gattagccat
     2641 catatccttt aacatcacct cttcttccgg agttaaaacc tgttccggct cttgagattt
     2701 agagtcctct agttcagagt ctattattgt ttttatatct tccatgaaaa ttaaacgccc
     2761 tatgcatata agagcgtggt tataaatact acaacatgga ggattgataa tcaagtctta
     2821 ttttcttcga tttttcatcc tccattcctc aatatcttta tgatggccgg agagaagaac
     2881 tagcggaact ctgtatttct tacccttata aacgatagtc tcgggcctgg tataactagg
     2941 tacgccgaca cccaaacgat tttcttccaa tgattctccc ttccctaaaa ctcccgaaat
     3001 ct
//
LOCUS       DATDNZ010000080         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1036273, whole genome shotgun sequence.
ACCESSION   DATDNZ010000080 DATDNZ010000000
VERSION     DATDNZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036273"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VI432_03100"
     CDS             complement(<1..543)
                     /locus_tag="VI432_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497108.1"
                     /translation="MRLGTITEDKLKDILIEGGYVDKADLTESVKVAEKESLPLISVL
                     LRRNLTTQKLIGQVIAEFYNLPYADLAARPPEKEMINIIPEDFAKNNRVVLLGVEGDK
                     VNVATDSPDRENLRQDLKKEIFDIFIKRKQLASLGQEEGFEIIVPQAPEFNIFYALPD
                     NIDSVFSQYRKALETKFVEII"
     gene            complement(548..907)
                     /locus_tag="VI432_03105"
     CDS             complement(548..907)
                     /locus_tag="VI432_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4497109.1"
                     /translation="MKVLVVEDEVPLAKTISAKLKINGFDTTVAFDGLEAIELLKAEK
                     FNFVVLDLIMPRADGFQVLEEMQKMDHKPPVFVMTALSQPEDEKRALNLGAVVYLRKE
                     TVSLKELIDKIKAYIGK"
     gene            992..2689
                     /locus_tag="VI432_03110"
     CDS             992..2689
                     /locus_tag="VI432_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4497110.1"
                     /translation="MLNPLSTPHEHKAKSSEEIRQNAYAAQTRLIYLFIFIGISFGII
                     LIIQRYLLEERVMAYIAIGFVVSLLADLIYLNIYKKNEIASTILLASFASFIVTFFIY
                     GAGNIFLWLPVFGAVAFFLKGTHMGFIISMTLYIIISILVLLDGLGKISIPFIKESIE
                     IAPDFLSSVIVLFLFMYLFHETMERNEKVIKEYSEEIEDKIEKERLILNSMETGLITL
                     NDKNIVDSINAYGANLLKINPDQGLGKNINDIIKFKKDGYDVAPEKNPIEFAKKKEGV
                     TKIRLSDKFELTSAISQKTFPIGMNITYPPENHDVKMIIVFFDLTQDKKIEQLKDAFL
                     SSMQHQITTPIATIRWGLELLREDFKNLETSGLTKEYIVDQVSHAVDRITQTQENIMT
                     VARIEHGFFPRIYKPLDIKKITDKVLEYVEASTQKTWVNINKVYDDNLQNIKSEETIL
                     FVILQNIISNAIKYNKVGGEIFINIVQSENDEILIKVKDQGIGIPKEAQSKIFEKFFR
                     TANAKSSMTEGTGLGLYITKILVSHIGGKIWFESEEGKGTTFFITIPNNPPPEESGE"
     gene            complement(2726..2796)
                     /locus_tag="VI432_03115"
     tRNA            complement(2726..2796)
                     /locus_tag="VI432_03115"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2762..2764),aa:Ala,seq:tgc)
BASE COUNT          982 a          608 c          491 g          814 t
ORIGIN      
        1 aattatttca acgaatttag tctccaacgc ctttctatat tgagagaata cggaatcgat
       61 attatccggc aaggcataaa aaatattgaa ttccggagcc tgcggcacaa taatctcaaa
      121 accctcctct tgaccaagag aagcgagctg ttttcttttt ataaaaatat caaagatttc
      181 ttttttcaaa tcttggcgca aattttctct gtctggactg tcagtcgcta cattgacctt
      241 atcaccctca actccaagca aaacaacacg attattttta gcgaaatcct ctggaataat
      301 attaatcatt tccttctctg ggggacgcgc agccaaatcc gcatatggca aattataaaa
      361 ttcggcgata acctgtccta tcaatttctg agtagtaaga ttccttcgaa gcaaaacaga
      421 gatgagcggc aaggattcct tttcggccac cttaaccgat tctgttaaat cagctttatc
      481 aacgtatccg ccctcaataa gaatatcttt taatttatct tcagtaatag tccctaatct
      541 catatttcta ttttcctata tatgccttta ttttgtctat taactcctta agagacaccg
      601 tctccttcct tagatagact actgcaccca agttcagcgc ccttttctcg tcttcgggct
      661 gagaaagcgc agtcattaca aaaacaggag gcttatgatc cattttttgc atctcttcca
      721 atacctggaa accgtcagcc cgaggcatga tgagatcaag cacaacaaaa ttgaatttct
      781 ctgctttcaa gagctctata gcctctaagc catcaaaggc aaccgttgtg tcaaaaccgt
      841 taattttcaa tttcgcggag atagttttgg ccaaaggaac ttcatcctct acaactaata
      901 ctttcatcaa gtccataatt aatttttaaa cagtatatca cagtttaaac ggcacaaaaa
      961 aatgcggtcc gtgatataat caacaaatac catgcttaat ccactctcta cgccacacga
     1021 acacaaagca aaaagctctg aagaaataag acagaacgcc tacgcggccc agacgcgttt
     1081 aatatatctc tttattttta ttggcatatc ttttggaatc atacttatca tacaacgcta
     1141 tctgctagaa gagcgggtta tggcttatat cgctattgga tttgtagttt cccttctagc
     1201 tgatctgata tatctcaata tctacaagaa aaacgaaata gcctctacca tactccttgc
     1261 ttcattcgcc tcttttattg tcacgttttt tatatatggc gccggaaata tattcttatg
     1321 gcttcccgta ttcggagcgg tcgctttctt cttaaaaggg actcatatgg gctttatcat
     1381 ctccatgacg ctctatataa taatctctat tttggtttta ttagacggac taggcaaaat
     1441 atctatacct tttattaaag aatcgattga gatagcgcct gattttcttt cgagtgtaat
     1501 tgtgcttttc ctttttatgt atctattcca tgagacaatg gaaagaaatg aaaaagttat
     1561 aaaagaatac tccgaagaga ttgaagataa gatagaaaaa gaaaggttaa ttttaaactc
     1621 gatggagact ggactgataa cgcttaatga taaaaatatt gtagattcaa taaacgccta
     1681 cggcgccaat ctactaaaaa taaatccaga ccaagggtta ggtaaaaaca tcaacgacat
     1741 aatcaaattc aaaaaagacg gttatgatgt agcgcctgaa aaaaacccga ttgaattcgc
     1801 caagaaaaaa gaaggagtca ccaaaataag actgagtgat aaattcgagc taacaagcgc
     1861 aatttcccaa aaaacctttc ccatcggaat gaacatcaca tatccgccgg agaaccacga
     1921 tgtcaaaatg ataatcgtat tctttgatct gacgcaggat aaaaagatcg aacagttaaa
     1981 agacgcattc ttatcctcaa tgcaacacca gatcacaact ccaatcgcaa caataagatg
     2041 gggccttgag cttttacgtg aagattttaa aaacctggaa acaagcggtc tcacgaaaga
     2101 atatatagta gaccaggtta gccatgcagt ggatagaatc acccagacac aagaaaatat
     2161 aatgaccgtt gcccgcatag aacacggttt cttccccaga atttataaac ccctggacat
     2221 taaaaaaatc acagacaaag tattggaata tgtggaagca agcacgcaaa aaacatgggt
     2281 caatataaat aaggtctatg atgacaacct acagaacata aaaagcgagg aaacaatatt
     2341 atttgtcatt cttcaaaata taatctcaaa cgctattaaa tataataagg ttggcggaga
     2401 aatttttatt aatatagtac agagtgaaaa cgacgaaata ttaatcaagg taaaagatca
     2461 aggtatcggc attcctaaag aggctcaatc aaaaatattt gaaaaatttt tccgcaccgc
     2521 aaatgccaaa tccagtatga ccgagggcac agggttgggt ctttatataa ctaaaatact
     2581 cgttagccat atcggaggta agatatggtt tgaatctgaa gagggcaagg gaacaacatt
     2641 ttttataacc atcccaaaca accctccgcc cgaagaaagc ggagagtaaa aataaacccg
     2701 ccatggcggg tttattttta tgttgtggac cctggcagaa tcgaactgcc gatccctcat
     2761 tgcaaatgag gagttatacc atttaactaa gggcccaatg tcaatcgagt ttaaactaaa
     2821 aatggatttt tggcaaaacc aaccggctgc acctcggcct ctaccactac tccaaattta
     2881 tttttaaccg cctct
//
LOCUS       DATDNZ010000081         9009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_1041950, whole genome shotgun sequence.
ACCESSION   DATDNZ010000081 DATDNZ010000000
VERSION     DATDNZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041950"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VI432_03120"
     CDS             complement(<1..1023)
                     /locus_tag="VI432_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497111.1"
                     /translation="MINRYFQYKNRSINRNSIKKYRDKLSPYIKAVSRKLDSHKYDSK
                     ESFILAPREGYGELKRFIKKKTKPDLDSIILVGIGGSNLGAMAVYEALSAKRKLIFVD
                     GLYPSKLEEVKKYIKEAYLKNKHCLLLLVSKSGKTTESIVNFNVLLNIFKRIDIRWKE
                     RVVIVSDEGSKLIEYADRNDFDYLTIPPVIEGRYSVFTAVGLLPLGLAGVDIDKLLRG
                     ARYGFEFALRDEFVNPAVIESASIMLSKKNIINMFLFDSRLSAFGLWWRQLIGESLGK
                     NGKGFTPIVSMGSVDLHSVAQLYFDGPKDKYTNFISIKRGDLDFKISNNKMLDGLVPG
                     LSGKSIS"
     gene            complement(992..2527)
                     /gene="zwf"
                     /locus_tag="VI432_03125"
     CDS             complement(992..2527)
                     /gene="zwf"
                     /locus_tag="VI432_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY4497112.1"
                     /translation="MYSYIGDPKNNIPTVFIILGATGDLVSKKVMPALFNLFSDGRLP
                     ELFKIIGFSRTEIGGEGFRDRVKNGLVHHFKSADVDSNKIEAFISHLDYQRSTFADLN
                     GYRSLSIKLEEVDQLWGLCSSKLLYLATPPNSFEEILNNLSKSGLNKVCGGGGGWTRI
                     ILEKPFGEDLKSARKLEKLLGKLFNESQIYRIDHYLAKEMTQNILSFRFSNNIFYPSW
                     NKRFIERIKIRVLEENGVEERGNFYDNVGALKDFGPNHILQMLALVTMDHPVRFDAES
                     IRKNRAKILSRLINPSVDYIKKNTFRAQYKGYKSINGVRENSETETYFKIKTFLNSTK
                     FKGIPIILESGKRMGKIRKDISIIFRHPTPCLCPGANHYKNKIVISLEPEEAITIQFG
                     SKKTGFNFELEERFFGFNLYADAGDKIRSRAGESYEKLLLDCIAGDQTLFVSTQEIIS
                     MWKFIDPIIRAWKDKSVELYYYKPDSSEASDLSKNMIEEETGIVNEDKVEINDQSLFP
                     IQK"
     gene            complement(2531..3238)
                     /locus_tag="VI432_03130"
     CDS             complement(2531..3238)
                     /locus_tag="VI432_03130"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4497113.1"
                     /translation="MEVLRFNSEMAKEKAAEEIGVLLSRTKVPVLLMLSGGSAFKILD
                     HIDMRNINSHVTITVLDERFSKDVKVNNFSQLMETTFGQVAAIRKASFIDTRVAEGED
                     LRMLADRFEKSVRQWEKDNKNGEIVVTQGIGSDGHTAGIMPYPEDKDKFIDLFGGKND
                     WIIGYNAGDKNQYPERITVTPSFLKDEVDHSIVFVGGEEKRNALDRIIFEKGAEYDTP
                     ATIIRGMKDVKIFTDIV"
     gene            complement(3385..3618)
                     /locus_tag="VI432_03135"
     CDS             complement(3385..3618)
                     /locus_tag="VI432_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY4497114.1"
                     /translation="MAKIIIYSTPTCVYCKLAKTFFNENSIAYEERDVTENDGWLDEM
                     VKKSNQMGVPVIDIDGSIVVGFDKNKVKELAGI"
     gene            complement(3669..3998)
                     /locus_tag="VI432_03140"
     CDS             complement(3669..3998)
                     /locus_tag="VI432_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497115.1"
                     /translation="MHKDLNIILPFLIAIILIGISFSISLIYGDIPGGNLIINYDYEE
                     NIPILGSERTINNIFYITLFILLINLLMTWIVYYRERFISYILSYSSVFISSLAFIAS
                     IFVYTIN"
     gene            complement(4004..4075)
                     /locus_tag="VI432_03145"
     tRNA            complement(4004..4075)
                     /locus_tag="VI432_03145"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4040..4042),aa:Glu,seq:ctc)
     gene            complement(4123..5760)
                     /locus_tag="VI432_03150"
     CDS             complement(4123..5760)
                     /locus_tag="VI432_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4497116.1"
                     /translation="MGFRVTLLIFIFAFLYSALTLNIYNLQLEEGDYYKAKAESIYRS
                     SGIFKPKRGNIYFTDKNENTIPASLNREYPTVYAIPEDVEDPFKAAKFIAETLELSEE
                     DLITSLSKKNDPYEPIAPKVADSIALAIKEFGEKGIHVGYEPFRFYPLGSIASHVLGF
                     VSTDVDNKIGLYGIESYYEELLNGSSGSIEEDIFVSPRDGKDLNLAIDRNIQTQSESI
                     LKELISDYSAKSGSVIVQNPKTGEILAMANFPNFDPNKYNEYSVKNFLNPSVQAIYEP
                     GSIFKVITMAIGLDAGKFTPETKYLDSGSITYNGRTIRNWDLKAHGQVTMTNVLEKSL
                     NTGAANAERLIGHDLFYDYLVKFGLKEQTGIDLPGEVAGSLKPLEESKRDINFATASF
                     GQGISVTPIRLINAISAIANGGVMMRPYLNKELKPEIEDRVISASASKEITDMMISAV
                     DKAEVAKINGYTVAGKTGTAQVPDFARGGYKDEVINTYVGFAPAHNPKFTILIKLDEP
                     SGAPLAGLTVVPAFREIAQFILNYYSVPPDRTDKAIR"
     gene            complement(5736..6029)
                     /locus_tag="VI432_03155"
     CDS             complement(5736..6029)
                     /locus_tag="VI432_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497117.1"
                     /translation="MTVIRPNRKNNIIKLILVLSGVLMVVFGSGVHIYSDVVSLKHEG
                     EIGQDRLNAETVKNAELKNTLYAMIDPSNLAEVALKRGLIKDKNPQWAFASRY"
     gene            complement(6043..6975)
                     /gene="rsmH"
                     /locus_tag="VI432_03160"
     CDS             complement(6043..6975)
                     /gene="rsmH"
                     /locus_tag="VI432_03160"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY4497118.1"
                     /translation="MLQDSIHVPVLLNEVLEVLDPKPGEFFIDGTVNGGGHTFSIVQK
                     MMPNGKYLAVDLDGRILQGTRKMIEEKFQIPNSKFQILWEEGNYADLPKILEEKKLGR
                     ADGFLLDLGFSSMQLEARRGFSFRIEKGLSADLEPLDMRYSSESDITAAEIINSFREE
                     ELIDIFRNYGEERSARKIAKAILEGRKKERIVSVGQLVRVIDGVVLPKNRKDVLQKIF
                     QSLRIYVNDELNNLKSILENLAKVVKPGGRIAIISFHSLEDRLVKNKFKELVDERMAE
                     ILTHKPIEASEEEIKANPKARSAKLRAVKIFLKS"
     gene            complement(7076..7507)
                     /gene="mraZ"
                     /locus_tag="VI432_03165"
     CDS             complement(7076..7507)
                     /gene="mraZ"
                     /locus_tag="VI432_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY4497119.1"
                     /translation="MFIGEFKHNIDTKGRVAIPAKFRIKVSGGAIITRGLDHCLFVFT
                     IKDWETLAQKLVALPLAQANSRAFVRLMLSGASDVELDSQGRILIPDYLRKYAGLNKE
                     TKITGVYNRMEIWDSESWQRYKTKTEGSSEEIAEKLGELGI"
     gene            complement(8228..8593)
                     /gene="rplL"
                     /locus_tag="VI432_03170"
     CDS             complement(8228..8593)
                     /gene="rplL"
                     /locus_tag="VI432_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY4497120.1"
                     /translation="MDKDKFISEIEKLTVVELAELVKAIEEKFGISAAMPVAAGAAGG
                     GDAAVAEKTSFNVMLKAAGDQKINAIKAVKEVTGLGLKEAKDLVEGAPKMVKEGLKKE
                     EAEEMKKKLEAAGATVELQ"
     gene            complement(8697..>9009)
                     /locus_tag="VI432_03175"
     CDS             complement(8697..>9009)
                     /locus_tag="VI432_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4497121.1"
                     /translation="IILKDRNIDFDTKLSAGQLGTVFAKGDISEIAQNVYKFSKDKPN
                     FKMVGGIDVEKAEILQAETVNAIGRLPSKEILLGQLLGALTGPLRAFMYVLSERSKKL
                     Q"
BASE COUNT         2664 a         1983 c         1325 g         3037 t
ORIGIN      
        1 gctaatactt ttaccactaa gaccaggaac gagaccgtct aacattttat tatttgatat
       61 tttaaaatct aaatcccctc ttttaatcga tataaaatta gtatatttat ctttcgggcc
      121 gtcaaaataa agttgagcga cagagtgtaa gtcgaccgaa cccatcgaaa ctataggagt
      181 aaaaccctta ccattcttgc caagactttc tccaatcaat tgcctccacc atagaccaaa
      241 agcgctcagc cggctatcaa acaaaaacat attaataata tttttcttac tgagcataat
      301 tgatgcggat tctattaccg cgggattgac aaattcatcc cgcaacgcaa actcaaatcc
      361 ataacgtgcg ccccgtagca atttatctat atcaacccca gccagaccca atggcaataa
      421 accgacagca gtaaataccg agtatctgcc ctcgataact gggggaatag ttaaataatc
      481 aaaatcgttt ctatcggcat attcaattaa ttttgagcct tcatcgctta caataaccac
      541 ccgttctttc caccgaatat caattctctt aaaaatattt aaaagcacgt tgaagttaac
      601 aatgctttct gtggtttttc cggacttact gactaaaagc agcaaacaat gtttattttt
      661 taaataagcc tcttttatat atttcttcac ttcttccagc ttgctgggat ataaaccgtc
      721 tacaaaaatt aattttcttt ttgcggatag cgcctcataa acagccatcg ctcccagatt
      781 agaaccgccg attccaacaa gtataatact atctaaatcc ggctttgttt ttttcttaat
      841 aaatcttttt aattcaccgt agccttctct aggggcaaga ataaaactct cttttgaatc
      901 atatttatga gaatcaagtt tacgagaaac ggctttaata tatggagata gtttatctct
      961 atacttctta atagagtttc tattgataga tctatttttg tattggaaat aacgattgat
     1021 catttatttc tactttatct tcattgacta tgccagtttc ttcttcaatc atatttttag
     1081 acaaatcaga agcctcggag ctgtcaggtt tataataata taattccaca gacttgtcct
     1141 tccatgcgcg tataatcgga tcaataaatt tccacataga aataatttct tgtgtgctta
     1201 caaaaagtgt ttgatctcca gctatgcaat ccagtaaaag tttttcatag ctctcgccag
     1261 ctcttgatct aattttgtca cccgcatcag cataaagatt aaaaccaaaa aatctttctt
     1321 ctaactcaaa attaaaacct gttttcttcg atccgaactg tattgttatc gcttcttccg
     1381 gctctaaaga tataacgatt ttattcttat agtgattagc gccaggacaa agacaaggcg
     1441 taggatgtct aaatataata gaaatatctt ttcttatctt tcccatcctc ttcccgctct
     1501 caagtattat aggaattccc ttaaattttg ttgaatttaa aaaggtttta atcttaaaat
     1561 aagtttccgt ttccgagttt tctcttactc cattgataga cttatatcct ttatattggg
     1621 ctctaaacgt atttttttta atataatcga cgctcgggtt aatgagtctc gataaaattt
     1681 ttgctctgtt tttccttata gattcagcgt caaatctaac aggatggtcc atcgtcacaa
     1741 gagcaagcat ttgtaaaata tgattaggac cgaaatcttt tagagccccg acattatcat
     1801 aaaaattgcc cctctcttcc accccattct cttccagtac ccttatttta atcctctcaa
     1861 tgaatctttt attccagctc ggataaaaaa tattattaga aaatctaaag cttaagatgt
     1921 tttgcgtcat ttctttagcc aaataatggt caatcctata aatctgactt tcattaaaaa
     1981 gtttgcccaa taatttctct aattttcgcg cgctttttag atcttctcca aacggctttt
     2041 caagaataat tctggtccat ccccctcctc caccgcaaac cttattcaag cctgattttg
     2101 ataaattatt taatatttct tcaaaagaat tcggcggagt agcaagatat aataatttac
     2161 ttgaacacag accccataat tgatccacct cttctaattt gattgataaa ctacgataac
     2221 cattcaaatc tgcgaaagtt gacctctgat aatcgagatg gcttataaat gcctcgattt
     2281 tgttcgagtc aacatcagca ctcttaaaat gatggacaag tccatttttt accctgtctc
     2341 taaaaccttc tccgccaatt tctgtgcgtg agaaacctat tattttaaaa agctcgggca
     2401 gcctaccatc gctaaataaa ttaaacaaag cgggcatcac ctttttagaa accaaatcgc
     2461 cagtagcacc caggattata aatacggttg gaatattgtt ttttggatcg ccaatataag
     2521 aatacattgt ttaaactata tcagtaaaaa tttttacatc tttcattcct cttataatag
     2581 tcgctggcgt atcatactca gctccttttt caaaaataat cctgtctaaa gcatttcttt
     2641 tctcttctcc tccaacaaag acgattgagt gatcaacctc atcttttaaa aatgagggtg
     2701 tgacggttat tctttccgga tactgatttt tatctcccgc attataaccg ataatccaat
     2761 cgtttttccc gccgaacaaa tcaataaatt tatctttatc ctcaggataa ggcattatac
     2821 ccgcagtgtg tccatcagaa ccgatccctt gagtcaccac aatttctccg tttttattat
     2881 ctttttccca ttgacgcaca gatttttcaa aacgatcagc taacattctt agatcctctc
     2941 cctccgcaac tcgagtgtct ataaaactag cctttcgtat tgcggctact tgtccaaaag
     3001 tagtttccat caactgactg aaattattta ctttaacatc ctttgagaat ctttcatcta
     3061 aaacggtaat agtcacatga ctatttatat ttctcatatc tatatgatcc aaaattttaa
     3121 acgctgatcc gccagataac attaataaaa ctggtacttt agtgcgacta agcaggacgc
     3181 ctatttcttc agccgctttt tctttcgcca tttccgagtt gaatcttaaa acttccatat
     3241 cttttaagtt aatctatttc ttggattcga tattgttttt ttctggacta ttttcttttg
     3301 gcatatgatc tttaataatg gcacataaaa tatacgccaa atcttggcgt tattctataa
     3361 atcgcggatt tttcacagaa atatctaaat tccagccaat tctttgactt tattcttatc
     3421 aaatccaaca acgatgcttc catcaatatc tataaccggc acacccatct gatttgattt
     3481 cttgaccatt tcatcaagcc aaccatcatt ttctgtaaca tccctttctt cataagcaat
     3541 gctgttctca ttaaaaaacg tcttcgcaag tttacaataa acacaagtcg gagttgaata
     3601 aattataatt ttagccatgt ttatatttta taacataatt attacccttg ctaatgttaa
     3661 tatcttaatc aatttatagt gtaaacaaag atagacgcta taaaagctaa agagctgata
     3721 aaaacgcttg aatacgaaag aatataggag ataaaacgtt ccctataata aactatccaa
     3781 gtcatcaata aattaattaa taaaataaag agggttatat aaaagatatt atttatagtt
     3841 ctttcgctac cgaggatagg gatgttttct tcataatcat aattaataat caagtttcca
     3901 ccaggaatat ctccataaat gagagatatg gaaaaagata ttccaattaa gataattgcg
     3961 atcagaaaag gcaaaattat atttagatct ttgtgcatga tagtagtccc aaggggaatc
     4021 gaacccctgt tccatccttg agaaggatgt gtcctagccg ctagacgatg ggaccttggt
     4081 aaataatata aacaaagata tcttaataga caaatgaatc tcttacctga tcgccttatc
     4141 cgttctgtca ggcggaacac tataataatt taaaatgaat tgggcaattt ctctgaacgc
     4201 aggaacaaca gtaagcccag cgagcggcgc gccggagggc tcatcaagct taatgagaat
     4261 agtaaattta ggattgtggg ccggagcaaa tccgacataa gtgtttatga cttcgtcttt
     4321 atatccaccg cgcgcaaaat cgggcacttg cgccgtgcca gtttttccag cgaccgtata
     4381 tccattaatc tttgcaactt cagccttatc aacggcggaa atcatcatat cggtaatttc
     4441 tttgcttgct gatgcggaaa taaccctatc ttctatttcc ggtttcaatt ctttatttaa
     4501 atatgggcgc atcatcactc ctccatttgc aatagcagat atagcgttaa taagcctgat
     4561 aggagtaaca gatattcctt gaccaaaact tgctgtcgca aaatttatat cgcgcttact
     4621 ctcctcaagc ggttttaaac tgccagcaac ctccccaggc aaatctattc ccgtctgctc
     4681 ttttaatcca aacttaacaa gataatcata aaaaagatca tgtccaatca aacgctctgc
     4741 gttcgccgcg ccggtgttca aagacttttc taaaacattt gtcatcgtca cttgaccatg
     4801 agcttttaaa tcccagtttc ttatagttct gccgttataa gtaattgatc ctgaatcaag
     4861 atatttggtt tctggtgtga atttaccagc atcaagaccg attgccatag taataacctt
     4921 gaaaatagat cctggctcat aaatcgcctg aacggaagga ttcaaaaaat ttttaacact
     4981 atattcatta tatttgttag ggtcaaaatt aggaaaatta gccatagcaa ggatctctcc
     5041 cgttttagga ttttgaacaa tcacagatcc acttttagct gaatagtcac tgattaattc
     5101 ctttaatatg ctttctgatt gagtttgaat atttctatct attgcaagat ttaaatcttt
     5161 tccgtctctt ggcgaaacaa aaatatcttc ttctatagaa ccgctagaac cattcagaag
     5221 ctcttcataa taagactcta tgccatataa accaatttta ttatctacgt ctgtcgatac
     5281 aaaccccaag acatgagacg cgatagatcc aaggggataa aaacgaaacg gctcataacc
     5341 aacgtggatt cccttttctc cgaattcttt aatagccaat gctatagaat ccgccacttt
     5401 cggagcaatc ggctcatatg gatcgttctt tttggaaagt gatgttatta aatcttcttc
     5461 cgataattca agtgtctcag caataaattt agcggcttta aaggggtctt ctacatcttc
     5521 cggaattgca taaacagttg ggtattctct gtttaaagaa gctgggatag tattttcatt
     5581 tttatcggta aaataaatat tgcctctttt gggtttgaat attcctgaag aacgatagat
     5641 agattcggct ttagccttat aataatcgcc ctcttctaat tgaagattat aaatattaag
     5701 agttaatgct gaatacagaa atgcgaatat aaaaattaat aacgtgacgc gaaagcccat
     5761 tgcggatttt tatctttaat caatcctctc ttcagagcaa cttctgcaag attagatgga
     5821 tctatcatag catataatgt atttttgagt tctgcgtttt tcaccgtttc tgcgtttagt
     5881 ctgtcttgtc cgatctcacc ttcatgtttc aacgaaacta catcagaata aatatgcact
     5941 ccgctaccaa atacaaccat caaaaccccg ctcaaaacga gaattaattt tataatatta
     6001 ttttttctgt ttggtcttat aactgtcata gttaattagt tattagcttt ttaaaaatat
     6061 ctttaccgcc ctcaactttg ccgacctggc tttagggttt gccttaatct cttcttcgct
     6121 cgcttcaatt ggcttatgtg ttaaaatctc cgccatccgc tcatctacca gctctttaaa
     6181 cttattctta accaacctat cctctaatga atgaaatgag attatcgcta ttcgacctcc
     6241 tggcttaaca acttttgcca aattctccag gatggatttc aaattattca attcatcgtt
     6301 gacataaatg cggagtgact ggaaaatttt ctgtaaaaca tcttttctat tctttggcag
     6361 cacgacccca tctataactc tcactaattg cccaaccgac acgattctct ctttttttct
     6421 cccctcaagg attgctttag caatttttct cgcagacctc tcttctccat aatttctgaa
     6481 tatgtctatc agctcttctt cgcgaaaaga atttataatt tcagccgcag taatgtcgct
     6541 ctcggatgaa tatctcatat cgagcggttc gagatctgca ctcaaaccct tctcgattct
     6601 gaaactaaac ccgcgccttg cctccagctg catagaagag aatcccagat caagcaaaaa
     6661 cccatctgcc cttcccagtt ttttttcttc caatatcttt ggcaaatcag cataatttcc
     6721 ttcttcccaa agaatctgaa atttagaatt gggaatttga aatttctctt caatcatctt
     6781 ccttgttccc tgcagaatgc gcccatccaa atctactgcc agatatttcc cgttcggcat
     6841 cattttttga acgattgaaa aagtatgtcc tcctccatta acggttccat ctatgaaaaa
     6901 ttctcctggc ttgggatcta atacttccaa cacctcattt aaaagaacgg gaacgtgaat
     6961 tgaatcttga agcatgaatt atgaatcacg aacactttca aattcttgtt tcatgcttct
     7021 tatttggtga tttaaatttt tgttcataat tcttaattcg ttattcatgt ttctattaaa
     7081 tacctagttc tcccaatttc tctgcgatct cttccgatga tccctcggtt ttcgttttat
     7141 atctctgcca gctttctgaa tcccaaattt ccattctgtt ataaactcct gttatctttg
     7201 tctctttatt taatcctgca tatttgcgca aatagtctgg tattaaaatt ctcccttgcg
     7261 aatcaagttc tacatcagat gctccggaga gcatcaatcg cacaaacgct ctggaattcg
     7321 cttgcgccaa aggaagagca actaattttt gggcaagagt ctcccaatct ttaatagtga
     7381 aaacgaacaa acaatgatcc aaacctcttg taataatcgc tccacccgaa actttaatcc
     7441 taaatttagc cggaatagcg actctgccct tcgtgtctat attgtgtttg aattctccta
     7501 taaacatgtt accgattcca agctactaat ttatgcgaat ctacgaaata attcgatttt
     7561 ccccagtatt ttgatttacc ttttatttgc atttatttgt agattcgagt gtatttatcc
     7621 tttcgcatcg aggttatccc cagaaaattg tggttatccc cagagttatc cacaattccc
     7681 cacttttcat atattataac gttcctatcc ccacaaagca acaaccccta tccacaattt
     7741 agggattcgt tttcgcttga caaattattc ataatatgat atatttatat atgggatcat
     7801 ccacatagga tgttctccag ctctttctaa gagaggattc cagtatgaac gcaaggagaa
     7861 ccttaggaac gataatccta gccgcaatca caatcgccct aatcttctta ggaatgatac
     7921 ggggggtttt catcgattcg acgggctggg gtgtcatcct tgatccagca atgatcattt
     7981 tgtctctgat catcgctata atgtgcatca tgtgggcttt ttcaatcacg tgccgaaaga
     8041 tcaaaaaaac actcaagtaa ttagccctga tgatctactc ctgttctttt tcaggccccg
     8101 agtaggacct cttaaaacta ctccccgagt catacgaccc gggctctttc gttttgagat
     8161 caaactaaaa catcccgatt taagtcggaa tgttttctat tctactatta aattctctaa
     8221 ttgttttcta ctgtaattct acagtagctc cggcggcctc caattttttc ttcatttctt
     8281 ccgcctcttc tttcttcaat ccctctttaa ccatcttcgg agcgccttct accaagtctt
     8341 tagcctcttt aagaccgagt ccagtcacct ccttaacagc tttgatggcg ttaatcttct
     8401 gatcgccggc ggccttcagc attacgttaa aagaggtttt ctccgccact gccgcatctc
     8461 cgccacctgc agcgcctgca gcaaccggca ttgcagcgct gataccaaac ttttcctcta
     8521 tcgcctttac gagttcagca agctcaacaa cggttaactt ctctatttcg ctaataaatt
     8581 tatctttatc catggtgtga tactaattta cgaatgcata cgaatgatac gaataaatac
     8641 taatattttt attcgtatac ctattcgttt tattcgtacc catttgtata ttggtattat
     8701 tgtaatttct tcgacctttc acttaataca tacataaatg ctctgagcgg tcccgtaagc
     8761 gcgcccaaaa gctgtccaag cagtatttct tttgatggca atcttccgat agcattaact
     8821 gtttcagctt gtaatatctc agccttttca acgtcaattc caccaaccat tttaaaattc
     8881 ggtttatcct ttgaaaattt ataaacattt tgagcgattt ctgagatatc gccttttgcg
     8941 aagactgtcc cgagctgtcc tgcagaaagc ttagtatcaa aatcaatatt tctatcctta
     9001 agaataatt
//
LOCUS       DATDNZ010000082         3404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3658277, whole genome shotgun sequence.
ACCESSION   DATDNZ010000082 DATDNZ010000000
VERSION     DATDNZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658277"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..648
                     /locus_tag="VI432_03180"
     CDS             106..648
                     /locus_tag="VI432_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HEY4497122.1"
                     /translation="MEDIYTGEFSAKLKTIISSFKEDLLMLRANRPTPKLVEDIKVDY
                     SGQMLAIKQLGSISVEAPRDIVITPWDKNLIGQMHKAIEEANLGVSVSTQAASVRVKL
                     PELTEERKIELSKIIKSSAETNRIKARSLRDDMNKKTKDIKDEDERFRIKESLQKTID
                     KFNAEIESAVKDKLGDIEKS"
     gene            648..1652
                     /locus_tag="VI432_03185"
     CDS             648..1652
                     /locus_tag="VI432_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY4497123.1"
                     /translation="MLIVIFIITLGVLIVGHEFGHFIVAKLFKLRVDEFGFGFPPKIA
                     SKKVGETEYSFNLLPFGGFVKIYGEHQEENGENNENKKISFYHQNAPRRIAVISAGVI
                     MNFIIAWFAFSLVFAIGIPQAVYIESVVEGSPAALAGLKEGDIISEFKTIDEFKGFIN
                     QNLEKKVIVNEKEIIPRENPPEGEGAIGVSLREGGIEKQGAFQSLYSGFTTSVDMVGR
                     IFSAFGGMIKDALQGNGETLASVSGPVGIYNFFSVSQSLGFIYLIQFIGIISINLAVF
                     NLLPVPALDGGRLLFIGIEKIIGRRLNYKYEGVANALGFVFLILLAVIVTISDISKLI
                     "
     gene            1656..2876
                     /locus_tag="VI432_03190"
     CDS             1656..2876
                     /locus_tag="VI432_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY4497124.1"
                     /translation="MKQSELYIKTLRESPADEEAINAKLLIRAGFIHKEMAGVYSFLP
                     LGLRAIKKIEAIIREEMDGIGGNEILMPVLQPKENWEKTKRWESFDVLYKVQGQNGKW
                     MALGPTHEEIIVPLAKKYINSYRDLPSYYYQIQTKFRDEPRSKSGLLRGREFIMKDFY
                     SFHRDENDLNSFYKKVMDAYKIIFERIGINAVLTEASGGAFSKHSHEYQVVSDVGEDI
                     IFTCASCGFAKNKEISEVKNGDKCPNCGNIIEEKRAVECGNIFKLGTRFSEAFDLKYK
                     DEQGNEHFPYIGCYGAGIGRILGVIAELNHDKNGLIWPERIAPFKIHLLEFKNDLGKD
                     LYGRLARLQGGKNNVLYDDRDITPGEKLKDSDLIGLPYRAIISEKTGDKIELKKRNED
                     KTQLVTHEFLEKLL"
     gene            2851..>3404
                     /locus_tag="VI432_03195"
     CDS             2851..>3404
                     /locus_tag="VI432_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4497125.1"
                     /translation="MNFLRNFYKNVGVDLGTANILMFLDNKGIVINEPTLVAVNNRTN
                     QIISVGNDAKKMLDRTPAHISLIKPLMNGVISDFETTEQILKYFLNKANKREFFSRYN
                     IGAISVPTNLTEVERKSVEDAAISAGVSKVYLIEAPIAGAIGAGLPIEEPTSNMIIDI
                     GGGTTEISIISAGGVVVSKSLKIAG"
BASE COUNT         1245 a          580 c          703 g          876 t
ORIGIN      
        1 tataaaggtt ataattaata gattaaaaag catgtcagaa tataatacta acaaacgtct
       61 tcaactggag tcggcattat atcaattcaa taaataataa acactatgga agatatatac
      121 acaggagagt tcagtgccaa actgaaaaca ataatctcaa gctttaaaga ggatctgtta
      181 atgttacgcg ccaaccgccc gacacccaaa ctggtagaag atattaaagt agactactcc
      241 ggccagatgc tcgcgataaa acaattagga tcaattagcg ttgaggcgcc aagagacata
      301 gttataactc catgggataa aaatttaata ggtcagatgc ataaagctat agaagaagct
      361 aatcttggag taagcgtttc aacccaagcc gcatccgtca gggtaaaatt accggaatta
      421 acagaagaga gaaagattga attgagcaaa ataataaaat cgtcagccga gacgaatcga
      481 ataaaagcgc gatcactaag agacgatatg aacaaaaaaa caaaagacat aaaagacgag
      541 gacgaaagat tcagaattaa agaatctctg caaaaaacca ttgataaatt caatgcagaa
      601 atagaatctg ccgtaaaaga caagcttgga gacatagaaa aatcataatg ctaattgtta
      661 tctttataat caccctggga gtacttattg tcggccacga gttcggccat tttatcgttg
      721 cgaaactttt taaattaaga gttgatgaat ttgggtttgg ctttcctcca aaaatagcaa
      781 gcaaaaaagt cggagaaact gaatatagct ttaatctatt gcctttcggc ggctttgtta
      841 aaatttatgg agaacatcaa gaagaaaatg gagaaaacaa tgaaaataaa aaaataagct
      901 tctatcacca gaatgcgccc agaagaatcg ccgttatttc agcaggggta ataatgaatt
      961 ttattatcgc ttggtttgct ttcagccttg tttttgcgat aggaattcca caagctgtat
     1021 atattgaatc tgtagtagaa ggcagtccgg ctgctctcgc tggattaaaa gaaggcgata
     1081 taatatccga atttaaaaca atcgatgaat tcaaaggatt tataaatcag aaccttgaga
     1141 aaaaagtaat agtaaacgaa aaagaaataa tcccaagaga aaatccacca gaaggagaag
     1201 gagcaatcgg cgtttctctt agagagggcg ggatagaaaa gcaaggcgcc tttcaaagct
     1261 tatacagcgg atttacaacg tccgttgata tggttggccg gatcttttct gctttcggtg
     1321 ggatgattaa agatgccctg cagggaaatg gagaaacact tgctagcgtt tccggtccag
     1381 ttgggatata taactttttt agcgtttcgc aatcgcttgg attcatttac cttatccagt
     1441 ttataggaat aatttctata aatctggctg tcttcaacct tttgcccgtg ccggctctag
     1501 atggcggacg actgcttttt ataggaatag aaaaaataat aggcagaaga cttaattata
     1561 aatacgaggg cgtagctaac gcattaggat ttgttttttt gatactgctc gccgtaattg
     1621 taaccataag cgacataagc aaattgattt aaaacatgaa gcaatctgaa ttatatataa
     1681 aaactcttag agaatctccg gctgacgaag aagcaattaa tgcaaaactt ttgataagag
     1741 ccggcttcat tcataaagag atggctggag tttattcttt tctgccttta ggactgcgcg
     1801 ctataaaaaa aattgaggca ataataagag aggaaatgga cggtataggt ggcaacgaaa
     1861 ttctaatgcc ggttttacag cctaaagaaa actgggaaaa aacgaaaaga tgggaaagtt
     1921 ttgatgtctt atacaaagta caaggtcaaa acggcaagtg gatggcttta ggaccaacgc
     1981 acgaagaaat aatcgttcct ttggctaaaa aatatataaa ttcataccgc gatttaccct
     2041 cgtattacta tcaaattcaa actaaattca gagatgagcc aagatctaaa tccggcctat
     2101 tgaggggcag agaattcata atgaaagatt tttacagctt ccatagggac gaaaacgatt
     2161 taaattcttt ctataaaaaa gttatggacg cctacaaaat aatttttgag agaatcggca
     2221 taaacgcggt cttgacagaa gcgtcgggtg gcgcattttc aaaacactct catgaatatc
     2281 aagtcgtgtc cgatgtagga gaagatataa tattcacttg cgcgtcgtgc ggttttgcaa
     2341 agaacaaaga aatatctgaa gtgaaaaacg gcgataaatg cccgaactgc ggaaatataa
     2401 tcgaagaaaa aagagcagtt gaatgcggaa atatatttaa attaggaacc aggttctcag
     2461 aggcttttga tttaaagtat aaagacgaac aaggcaacga acactttcct tatattggct
     2521 gttatggcgc tggcatcgga aggatattag gcgttatagc agagcttaac catgacaaaa
     2581 acggattaat ctggccggaa agaatagctc cttttaaaat ccacctgctc gaatttaaaa
     2641 atgatctagg caaagactta tatggacgtt tagctcgttt acagggtggt aaaaataatg
     2701 tactctatga tgatcgagat atcacccctg gagaaaaatt aaaagattca gacttaatcg
     2761 gactgccata tagagctatt atcagcgaga aaaccggaga taaaatagaa cttaaaaaaa
     2821 gaaacgaaga taaaacacaa ttagtaacac atgaatttct tgagaaactt ttataaaaac
     2881 gttggcgtag acttgggaac agcaaacata ttaatgttcc tagacaacaa aggaattgtt
     2941 attaatgaac ctactctagt cgcggttaac aatcgcacta atcagataat aagcgtaggc
     3001 aatgatgcaa aaaaaatgct cgaccgcacg cctgcgcaca tcagcttgat caagcctctg
     3061 atgaacggag ttatatccga tttcgaaaca acagagcaaa tattaaaata ctttctaaat
     3121 aaagcgaata aaagagagtt tttttcaaga tacaacatag gcgcgattag cgttcccact
     3181 aatttaaccg aggttgaaag aaaatcagtt gaagacgcag ctatctccgc cggcgtttca
     3241 aaggtatatc tgattgaagc tcctatagct ggggcaatcg gcgctgggct acccatcgaa
     3301 gaaccaacct ctaatatgat aattgatata ggcggaggaa cgacagaaat atcaataata
     3361 tccgctggtg gcgttgttgt atctaagagt cttaaaatag cagg
//
LOCUS       DATDNZ010000083         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U7880
            k141_3661155, whole genome shotgun sequence.
ACCESSION   DATDNZ010000083 DATDNZ010000000
VERSION     DATDNZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962650
            Sequence Read Archive: SRR3989295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/14/2024 22:12:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 644
            CDSs (total)                 :: 610
            Genes (coding)               :: 610
            CDSs (with protein)          :: 610
            Genes (RNA)                  :: 34
            tRNAs                        :: 33
            ncRNAs                       :: 1
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661155"
                     /isolate="SMAG_U7880"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.53 N 107.78 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VI432_03200"
     CDS             complement(<1..637)
                     /locus_tag="VI432_03200"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY4497126.1"
                     /translation="MEYAYLILFLTGVAFGSFLNVVSLRFNGEGKLFDNIYGRSECMS
                     CHKKLNWYELIPLFSFIIQLGKCRSCGVKLLWQYPLVEFLAGIIFIAVPCALIPNGVS
                     FIPYFSIVLWILVFLALLLMSVIDFRLQIIPDSINIFIGILGLANFIYIFLGGEFGAV
                     MDGIFGLLSDHPMVVGTYLGTHALMLSFWENVWVSFGLGILFGGGFFGSLYF"
     gene            complement(645..1190)
                     /locus_tag="VI432_03205"
     CDS             complement(645..1190)
                     /locus_tag="VI432_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4497127.1"
                     /translation="MIYSSRSGKNHNSLFMPHTSHKRGFTLIEMLVVIAIISVLAGVV
                     LTGVTGFQANARDTRRIGDLKNTQNFLELYFVKCGHYPGGYSGGACTTTAPANWVALK
                     TMMEAAPFSISNFPKDPVTARNYEYGVENPENLKYVLKASLERDNRVLDDDVDTIPFT
                     ATPSVMCGPDTTTNHFYCITP"
     gene            complement(1365..2573)
                     /locus_tag="VI432_03210"
     CDS             complement(1365..2573)
                     /locus_tag="VI432_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4497128.1"
                     /translation="MKFNYKARTKEGELQVGNIEAASRSAAATMLGGHDLFVLSIEEA
                     EKKHWYDFFLGFLNKVKVKDLMIFTRQFATLLSAKIPISDSLNTLRQQTRNPILKDTV
                     SDIQSDIDAGLSLSQSLEKYGDIFSVFYVNMVRSAELTGRVEESVNFLADYLEKETAI
                     ISKIRNALIYPAIMLVLFLIVAAFMAAVVLPKIEPVFIEAGVELPLFTKILIGGGKFL
                     ASWWWVVLLITAAFIALLVDYLRTPEGKNTFDEIILRTPVFGGILKQVYVARFAESVS
                     VLVKGGISMPQAMEVTGHTIGSTIYREMLHQTAEDLRKGELLSQSLSKNEKYFPPLLI
                     QMTAIGESTGRLEELLDRVSRFYSREVDSLLDNLVELIQPVLMVFIGLSVGALFASIL
                     IPIFQLAQRF"
     gene            complement(2570..2833)
                     /locus_tag="VI432_03215"
     CDS             complement(2570..2833)
                     /locus_tag="VI432_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4497129.1"
                     /translation="MTYYVYVLQSKRYGTWYTGYTNNLRKRFNEHNSKKDGYTKSRGP
                     YELIYYEACYNEDDARSRETYLKSGMGKRYLKNRLKRFLSLTG"
     gene            complement(2856..>3201)
                     /locus_tag="VI432_03220"
     CDS             complement(2856..>3201)
                     /locus_tag="VI432_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEY4497130.1"
                     /translation="DRIIDSFPAEQQGQVTSQLAATLVGIVSQRLVPRIDGGRIPACE
                     IMFMNSAIRNLIREKKIYQIDLVIETSLQEGMMTLNRSLAELVKRKEISLENAELYSL
                     NPSELRILLERI"
BASE COUNT         1036 a          730 c          537 g          898 t
ORIGIN      
        1 aaaaatataa tgagccaaag aacccgcctc cgaacaaaat tcccaagccg aaactaaccc
       61 aaacattctc ccagaacgag agcattaagg cgtgcgtacc gagatatgta cccaccacca
      121 tcgggtgatc gcttaaaagt ccgaaaatcc cgtccatgac cgcaccgaac tctcctccta
      181 gaaatatata aataaaattt gccaatccaa gaattcctat aaatatattg atgctgtcag
      241 ggattatctg cagtctaaag tctatgaccg acatcagaag aagagccaaa aaaaccaaaa
      301 tccacaagac gatgctgaaa tacggaataa atgacacgcc attaggaata agggcacatg
      361 gaaccgcaat gaaaataatt cccgccaaaa actctaccaa cgggtattgc cataaaagct
      421 ttacgccgca cgaacggcat ttacctaatt ggataataaa actaaaaaga ggaataagct
      481 cgtaccagtt aagcttcttg tggcaagaca tgcactctga cctaccgtag atattatcaa
      541 aaagctttcc ctctccatta aatcttaaag aaacaacatt tagaaaactg ccgaaagcaa
      601 ccccggttag aaaaaggatt aagtatgcgt attccattta acgactaagg ggttatacaa
      661 taaaaatgat tcgtagtcgt gtcaggacca cacatcactg atggcgtagc tgtaaacggg
      721 atagtatcta catcatcatc taacactcta ttatctctct ctaatgaagc cttcaaaacg
      781 tatttaaggt tctctgggtt ctcaactcca tactcataat tcctagctgt tacaggatct
      841 ttgggaaaat tactgatact aaaaggggcg gcctccatca tagtttttag agcgacccag
      901 ttagctggcg ctgttgtagt acatgctcct cctgaatatc cgccaggata atgtccacat
      961 ttcacgaagt acaattccaa gaaattctgt gtattcttta gatcgcctat acgcctggta
     1021 tctcttgcgt ttgcctgaaa accggtaaca ccggtcaaga cgactcccgc caaaaccgaa
     1081 atgatggcga taacaaccaa catttcaata agggtaaaac ccctcttatg tgaagtatga
     1141 ggcatgaata atgaattatg atttttacca gatcttgaac tatagatcat gtctaaaaag
     1201 attataaact aattaaaatt tctgcgctaa tggaaatctg tgcataactc caggacttga
     1261 ataacgaagc aagaattatc aattacgctt attatgatta ctgctttcta tagtttcttc
     1321 tcttatttaa aattcttgat tcctgcttct tgattcataa ttaattagaa tctctgcgct
     1381 aactggaata tcggaattag aattgaagca aacagagctc caaccgaaag cccgataaaa
     1441 accatcagaa ctggctgaat aagctcaacc aaattatcaa gcaggctatc aacctcacgc
     1501 gaataaaaac gcgaaactct atctaaaagc tcctctaatc ttccggtaga ttcaccaatc
     1561 gccgtcatct gtattagaag tggaggaaaa tacttctcat tcttggagag agattgagaa
     1621 agcaactctc cctttcttaa atcttccgct gtctgatgaa gcatttctcg ataaatcgtg
     1681 cttccaattg tatgtccggt aacctccatc gcttgaggca tagatattcc ccccttgact
     1741 aagacactca cagattctgc gaatctcgca acataaactt gctttagaat tccgccaaat
     1801 acgggtgtac gcaagattat ctcatcaaac gtatttttcc cctctggtgt tcttaaataa
     1861 tcaacaagaa gagcaataaa agcggctgta ataagcagaa caacccacca ccatgatgcc
     1921 aagaacttgc ctccgccaat caaaatcttt gtgaacagcg gtaattcaac gcctgcctct
     1981 ataaaaaccg gctcaatttt aggcaaaaca accgcggcca taaatgccgc aacgatcagg
     2041 aataaaacaa gcatgatagc aggataaata agcgcattcc taattttaga aataatcgct
     2101 gtttctttct caagataatc cgctaaaaaa ttaaccgact cctctactct gccagtcaat
     2161 tccgcagatc taaccatatt cacatagaaa acagaaaaaa tatctccata tttttcaaga
     2221 gattgggaaa gtgaaagtcc ggcatcaata tcgctttgaa tatcagaaac cgtgtctttt
     2281 aaaatcgggt ttcttgtctg ctgtcttaga gtatttaaag aatcgctgat tggaattttt
     2341 gccgagagaa gtgtggcaaa ttgcctagtg aaaatcataa gatctttgac tttgacctta
     2401 ttcaaaaacc ctaagaaaaa atcataccaa tgctttttct ctgcttcctc gattgatagg
     2461 acaaaaaggt cgtgtccgcc aagcattgta gccgcagcgc tacggcttgc cgcttcaata
     2521 ttaccaacct gcagttctcc ttctttagtt cttgctttgt aattaaactt catcccgtta
     2581 gagataggaa acgttttaat ctatttttaa gatatctctt gcccataccg gatttgagat
     2641 aagtttctct cgacctagca tcatcttcat tataacaagc ttcataataa attaattcgt
     2701 atggtcctcg gcttttagta tagccatcct ttttagaatt gtgttcatta aaacgtttcc
     2761 gtagattgtt agtatagcca gtataccaag ttccatatct tttgctctgt aatacataaa
     2821 cataatatgt catctctaac gggattcatg ctcgcctaga tgcgttccag taatatcctt
     2881 aactctgacg gatttaatga atacagctcg gcgttctcca aagagatctc ttttcttttc
     2941 acaagttcag caagagatct attcaaagtc atcatgccct cttgcaggct cgtttcaata
     3001 accaaatcaa tctgataaat ctttttctct ctaataagat ttctaatggc cgagttcatg
     3061 aacattatct cgcacgcggg aattctccct ccgtctattc tagggaccaa tctttgggaa
     3121 acaatcccaa caagagtagc agccaactgc gaggtcactt gcccctgctg ttcagccggg
     3181 aaagaatcaa taattctatc t
//
